LOCUS       DAVBCG010000001        63812 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBCG010000001 DAVBCG010000000
VERSION     DAVBCG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63812
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.001"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            212..3982
                     /gene="spuA"
                     /locus_tag="VQ495_000001"
     CDS             212..3982
                     /gene="spuA"
                     /locus_tag="VQ495_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2530028.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSNPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWIKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYRTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVRNESV
                     ENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            complement(4082..5131)
                     /locus_tag="VQ495_000002"
     CDS             complement(4082..5131)
                     /locus_tag="VQ495_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2530029.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(5250..7058)
                     /gene="glmS"
                     /locus_tag="VQ495_000003"
     CDS             complement(5250..7058)
                     /gene="glmS"
                     /locus_tag="VQ495_000003"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2530030.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGTDNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVVDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLIHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7250..8629)
                     /gene="bglA"
                     /locus_tag="VQ495_000004"
     CDS             complement(7250..8629)
                     /gene="bglA"
                     /locus_tag="VQ495_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2530031.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWDRDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(8728..10581)
                     /locus_tag="VQ495_000005"
     CDS             complement(8728..10581)
                     /locus_tag="VQ495_000005"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2530032.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALIEKLEASLMGDGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10594..11853)
                     /gene="rseP"
                     /locus_tag="VQ495_000006"
     CDS             complement(10594..11853)
                     /gene="rseP"
                     /locus_tag="VQ495_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2530033.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(11875..12678)
                     /locus_tag="VQ495_000007"
     CDS             complement(11875..12678)
                     /locus_tag="VQ495_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2530034.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12687..13445)
                     /locus_tag="VQ495_000008"
     CDS             complement(12687..13445)
                     /locus_tag="VQ495_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2530035.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13676..14248)
                     /locus_tag="VQ495_000009"
     CDS             complement(13676..14248)
                     /locus_tag="VQ495_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2530036.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(14229..15200)
                     /gene="ruvB"
                     /locus_tag="VQ495_000010"
     CDS             complement(14229..15200)
                     /gene="ruvB"
                     /locus_tag="VQ495_000010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2530037.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15361..15453
                     /locus_tag="VQ495_000011"
     CDS             15361..15453
                     /locus_tag="VQ495_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2530038.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            complement(16080..16496)
                     /locus_tag="VQ495_000012"
     CDS             complement(16080..16496)
                     /locus_tag="VQ495_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530039.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(16508..17203)
                     /locus_tag="VQ495_000013"
     CDS             complement(16508..17203)
                     /locus_tag="VQ495_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530040.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            complement(17397..19898)
                     /locus_tag="VQ495_000014"
     CDS             complement(17397..19898)
                     /locus_tag="VQ495_000014"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2530041.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            complement(20353..21441)
                     /locus_tag="VQ495_000015"
     CDS             complement(20353..21441)
                     /locus_tag="VQ495_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2530042.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(21459..22127)
                     /locus_tag="VQ495_000016"
     CDS             complement(21459..22127)
                     /locus_tag="VQ495_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2530043.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            complement(22146..24584)
                     /locus_tag="VQ495_000017"
     CDS             complement(22146..24584)
                     /locus_tag="VQ495_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2530044.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(24735..26033)
                     /locus_tag="VQ495_000018"
     CDS             complement(24735..26033)
                     /locus_tag="VQ495_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2530045.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQTLGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            complement(26050..26358)
                     /locus_tag="VQ495_000019"
     CDS             complement(26050..26358)
                     /locus_tag="VQ495_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2530046.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(26404..26724)
                     /locus_tag="VQ495_000020"
     CDS             complement(26404..26724)
                     /locus_tag="VQ495_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2530047.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            complement(26919..27899)
                     /locus_tag="VQ495_000021"
     CDS             complement(26919..27899)
                     /locus_tag="VQ495_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2530048.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            complement(27912..28658)
                     /locus_tag="VQ495_000022"
     CDS             complement(27912..28658)
                     /locus_tag="VQ495_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2530049.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYETKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRVIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     CQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAVSDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            28780..29556
                     /locus_tag="VQ495_000023"
     CDS             28780..29556
                     /locus_tag="VQ495_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2530050.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            29631..29828
                     /locus_tag="VQ495_000024"
     CDS             29631..29828
                     /locus_tag="VQ495_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2530051.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            30045..30641
                     /locus_tag="VQ495_000025"
                     /pseudo
     CDS             30045..30641
                     /locus_tag="VQ495_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            30717..31166
                     /locus_tag="VQ495_000026"
     CDS             30717..31166
                     /locus_tag="VQ495_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2530052.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            31179..32270
                     /locus_tag="VQ495_000027"
     CDS             31179..32270
                     /locus_tag="VQ495_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530053.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKESLFTLLGPSGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDIRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEELQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            32260..33912
                     /locus_tag="VQ495_000028"
                     /pseudo
     CDS             32260..33912
                     /locus_tag="VQ495_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(34015..34707)
                     /locus_tag="VQ495_000029"
     CDS             complement(34015..34707)
                     /locus_tag="VQ495_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2530054.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(34951..36288)
                     /locus_tag="VQ495_000030"
     CDS             complement(34951..36288)
                     /locus_tag="VQ495_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2530055.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINCAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(36298..36564)
                     /locus_tag="VQ495_000031"
     CDS             complement(36298..36564)
                     /locus_tag="VQ495_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2530056.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(36829..37215)
                     /gene="rplQ"
                     /locus_tag="VQ495_000032"
     CDS             complement(36829..37215)
                     /gene="rplQ"
                     /locus_tag="VQ495_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2530057.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(37227..38162)
                     /locus_tag="VQ495_000033"
     CDS             complement(37227..38162)
                     /locus_tag="VQ495_000033"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2530058.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(38205..38588)
                     /gene="rpsK"
                     /locus_tag="VQ495_000034"
     CDS             complement(38205..38588)
                     /gene="rpsK"
                     /locus_tag="VQ495_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2530059.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(38606..38971)
                     /gene="rpsM"
                     /locus_tag="VQ495_000035"
     CDS             complement(38606..38971)
                     /gene="rpsM"
                     /locus_tag="VQ495_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2530060.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(38989..39105)
                     /gene="rpmJ"
                     /locus_tag="VQ495_000036"
     CDS             complement(38989..39105)
                     /gene="rpmJ"
                     /locus_tag="VQ495_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2530061.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(39130..39348)
                     /gene="infA"
                     /locus_tag="VQ495_000037"
     CDS             complement(39130..39348)
                     /gene="infA"
                     /locus_tag="VQ495_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2530062.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(39465..40103)
                     /locus_tag="VQ495_000038"
     CDS             complement(39465..40103)
                     /locus_tag="VQ495_000038"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2530063.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(40254..41564)
                     /gene="secY"
                     /locus_tag="VQ495_000039"
     CDS             complement(40254..41564)
                     /gene="secY"
                     /locus_tag="VQ495_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2530064.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(41577..42017)
                     /gene="rplO"
                     /locus_tag="VQ495_000040"
     CDS             complement(41577..42017)
                     /gene="rplO"
                     /locus_tag="VQ495_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2530065.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(42162..42386)
                     /gene="rpmD"
                     /locus_tag="VQ495_000041"
     CDS             complement(42162..42386)
                     /gene="rpmD"
                     /locus_tag="VQ495_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2530066.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(42358..42852)
                     /gene="rpsE"
                     /locus_tag="VQ495_000042"
     CDS             complement(42358..42852)
                     /gene="rpsE"
                     /locus_tag="VQ495_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2530067.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(42870..43226)
                     /gene="rplR"
                     /locus_tag="VQ495_000043"
     CDS             complement(42870..43226)
                     /gene="rplR"
                     /locus_tag="VQ495_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2530068.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(43310..43846)
                     /gene="rplF"
                     /locus_tag="VQ495_000044"
     CDS             complement(43310..43846)
                     /gene="rplF"
                     /locus_tag="VQ495_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2530069.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(44038..44436)
                     /gene="rpsH"
                     /locus_tag="VQ495_000045"
     CDS             complement(44038..44436)
                     /gene="rpsH"
                     /locus_tag="VQ495_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2530070.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(44721..44990)
                     /gene="rpsN"
                     /locus_tag="VQ495_000046"
     CDS             complement(44721..44990)
                     /gene="rpsN"
                     /locus_tag="VQ495_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2530071.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(45008..45550)
                     /gene="rplE"
                     /locus_tag="VQ495_000047"
     CDS             complement(45008..45550)
                     /gene="rplE"
                     /locus_tag="VQ495_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2530072.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(45574..45879)
                     /gene="rplX"
                     /locus_tag="VQ495_000048"
     CDS             complement(45574..45879)
                     /gene="rplX"
                     /locus_tag="VQ495_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2530073.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(45957..46325)
                     /gene="rplN"
                     /locus_tag="VQ495_000049"
     CDS             complement(45957..46325)
                     /gene="rplN"
                     /locus_tag="VQ495_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2530074.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(46351..46611)
                     /gene="rpsQ"
                     /locus_tag="VQ495_000050"
     CDS             complement(46351..46611)
                     /gene="rpsQ"
                     /locus_tag="VQ495_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2530075.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(46636..46842)
                     /locus_tag="VQ495_000051"
     CDS             complement(46636..46842)
                     /locus_tag="VQ495_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2530076.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(46852..47265)
                     /gene="rplP"
                     /locus_tag="VQ495_000052"
     CDS             complement(46852..47265)
                     /gene="rplP"
                     /locus_tag="VQ495_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2530077.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(47269..47922)
                     /gene="rpsC"
                     /locus_tag="VQ495_000053"
     CDS             complement(47269..47922)
                     /gene="rpsC"
                     /locus_tag="VQ495_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2530078.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(47935..48279)
                     /gene="rplV"
                     /locus_tag="VQ495_000054"
     CDS             complement(47935..48279)
                     /gene="rplV"
                     /locus_tag="VQ495_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2530079.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(48291..48572)
                     /gene="rpsS"
                     /locus_tag="VQ495_000055"
     CDS             complement(48291..48572)
                     /gene="rpsS"
                     /locus_tag="VQ495_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2530080.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(48676..49509)
                     /gene="rplB"
                     /locus_tag="VQ495_000056"
     CDS             complement(48676..49509)
                     /gene="rplB"
                     /locus_tag="VQ495_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2530081.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(49527..49823)
                     /locus_tag="VQ495_000057"
     CDS             complement(49527..49823)
                     /locus_tag="VQ495_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2530082.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(49823..50446)
                     /gene="rplD"
                     /locus_tag="VQ495_000058"
     CDS             complement(49823..50446)
                     /gene="rplD"
                     /locus_tag="VQ495_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2530083.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(50471..51097)
                     /gene="rplC"
                     /locus_tag="VQ495_000059"
     CDS             complement(50471..51097)
                     /gene="rplC"
                     /locus_tag="VQ495_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2530084.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(51313..51621)
                     /gene="rpsJ"
                     /locus_tag="VQ495_000060"
     CDS             complement(51313..51621)
                     /gene="rpsJ"
                     /locus_tag="VQ495_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2530085.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(51887..52504)
                     /locus_tag="VQ495_000061"
     CDS             complement(51887..52504)
                     /locus_tag="VQ495_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2530086.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(52501..53091)
                     /gene="nrdG"
                     /locus_tag="VQ495_000062"
     CDS             complement(52501..53091)
                     /gene="nrdG"
                     /locus_tag="VQ495_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2530087.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKEIYEQVKRE"
     gene            complement(53084..53590)
                     /locus_tag="VQ495_000063"
     CDS             complement(53084..53590)
                     /locus_tag="VQ495_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530088.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(53634..53774)
                     /locus_tag="VQ495_000064"
     CDS             complement(53634..53774)
                     /locus_tag="VQ495_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530089.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(53786..55993)
                     /gene="nrdD"
                     /locus_tag="VQ495_000065"
     CDS             complement(53786..55993)
                     /gene="nrdD"
                     /locus_tag="VQ495_000065"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2530090.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNCRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(56113..57651)
                     /locus_tag="VQ495_000066"
     CDS             complement(56113..57651)
                     /locus_tag="VQ495_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2530091.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(57688..57831)
                     /locus_tag="VQ495_000067"
     CDS             complement(57688..57831)
                     /locus_tag="VQ495_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530092.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(57909..59441)
                     /gene="cls"
                     /locus_tag="VQ495_000068"
     CDS             complement(57909..59441)
                     /gene="cls"
                     /locus_tag="VQ495_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2530093.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(59587..60144)
                     /locus_tag="VQ495_000069"
     CDS             complement(59587..60144)
                     /locus_tag="VQ495_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2530094.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(60141..61391)
                     /locus_tag="VQ495_000070"
     CDS             complement(60141..61391)
                     /locus_tag="VQ495_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2530095.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFEMLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNFSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(61599..61904)
                     /locus_tag="VQ495_000071"
     CDS             complement(61599..61904)
                     /locus_tag="VQ495_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2530096.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(61920..62339)
                     /gene="ruvX"
                     /locus_tag="VQ495_000072"
     CDS             complement(61920..62339)
                     /gene="ruvX"
                     /locus_tag="VQ495_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2530097.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPLDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(62343..62609)
                     /locus_tag="VQ495_000073"
     CDS             complement(62343..62609)
                     /locus_tag="VQ495_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2530098.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(62696..63265)
                     /locus_tag="VQ495_000074"
     CDS             complement(62696..63265)
                     /locus_tag="VQ495_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2530099.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(63331..63729)
                     /gene="spx"
                     /locus_tag="VQ495_000075"
     CDS             complement(63331..63729)
                     /gene="spx"
                     /locus_tag="VQ495_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2530100.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT        18882 a        14185 c        11397 g        19348 t
ORIGIN      
        1 ttctgaataa aaatagctgg gaacacatca tcttcctaat aatacggctt ggcttatgtg
       61 acatttttat ctccgtcatt tatactgata tataaaatag tacagaaaac ttctcaaaag
      121 taggaatttt ttcacaaatc aaagaaaacg cttgcatatt gttttactac atgctatact
      181 gatatagaaa tttattgatt ggagaatcat tatgagaaaa acaccatctc acactgagaa
      241 aaaaatggtc tacagcatcc gttccctcaa aaatggaact ggttctgtcc ttatcggcgc
      301 aagccttgtc ttgcttgcca tggctacacc aactatctca tccgacgaaa gtacaccaac
      361 cactaacgaa cccaacaaca gaaatacaac cacccttgcc caacctctta ctgatacagc
      421 agctggctct ggtaagaacg aaagtgatat ttcttcacct agaaatgcaa acgcttccct
      481 agagaaaaca gaagaaaaac ctgcaacaga gccaactact tctactagtc cagtaacaac
      541 tgaaactaag gcagaagagc ccatcgaaga taactacttc cgtatccatg tcaaaaaact
      601 tcctgaagaa aacaaggatg ctcaaggact atggacttgg gacgatgttg aaaaaccatc
      661 tgaaaactgg ccaaacggag ctttgtcctt caaggatgcc aagaaagatg actacggcta
      721 ttacctagat gtcaaattaa agggagaaca agccaagaaa attagcttcc tcatcaacaa
      781 tacagctgga aaaaatctaa ccggcgataa atctgtagaa aaactagttc caaaaatgaa
      841 cgaggcttgg ttagaccaag attacaaggt tttctcttac gagccacagc ctgcaggaac
      901 tgttcgcgtc aactactacc gcacagatgg caactatgac aagaaatctc tctggtactg
      961 gggagatgta aaaaatccaa gtagcgctca atggcctgac ggaacagact ttacggctac
     1021 aggcaaatat ggccgctata tcgacattcc tcttaatgaa gccgcaagag aatttggatt
     1081 tttattacta gatgagagca aacaaggaga cgacgtgaaa atccgtaaag aaaattataa
     1141 gttcacagat ttgaaaaatc atagccaaat tttcctaaaa gacgatgatg aatcgattta
     1201 cacaaatcca tactatgtcc atgatatccg tatgacagga gcccaacacg taggcacttc
     1261 tagcattgaa agtagctttt caacacttgt cggtgctaaa aaagaagata tcctcaaaca
     1321 ctccaacatc actaatcacc taggaaacaa ggtaactatt accgatgttg caatcgatga
     1381 agctggtaag aaagtgacct acagcggaga tttctctgac acaaaacatc cttatactgt
     1441 tagctacaat tccgaccaat tcactaccaa aacaagctgg cgcctgaaag atgagacata
     1501 cagctatgat ggcaaactgg gagctgacct aaaagaagaa ggaaaacaag ttgatttgac
     1561 cctttggtca ccaagtgctg ataaggtttc tgttgttgtc tacgacaaga atgaccctga
     1621 caaagtagtt ggaactgtcg ctctcgaaaa aggggaaaga ggaacttgga aacaaactct
     1681 agacagcaca aacaaactcg gaatcacaga tttcactggc tactattatc aataccaaat
     1741 cgagcgtcaa ggtaaaactg ttcttgcact cgatccttac gctaaatctc ttgctgcttg
     1801 gaatagcgac gatgccaaga ttgacgatgc ccataaagtg gctaaagccg cctttgtaga
     1861 tccagctaaa ctcggacctc aagacttgac ttatggtaag attcacaatt tcaagactcg
     1921 tgaagacgcc gttatctacg aagctcatgt gcgtgacttc acttcagatc ctgccattgc
     1981 aaaagacttg accaaaccat ttgggacttt tgaagccttc attgaaaaac tagactatct
     2041 caaagacttg ggtgtaaccc atatccagct ccttccagtc ttgtcttact actttgtcaa
     2101 tgaattgaaa aaccatgaac gcttgtctga ctatgcttca agcaacagca actacaactg
     2161 gggatatgac cctcaaaact acttctcctt gactggtatg tactcaagca atcctaagaa
     2221 tccagaaaaa cgaatcgcag aatttaaaaa cctcatcaac gaaatccaca aacgtggtat
     2281 gggagctatc ctagatgtcg tttataacca cacagccaaa gtcgatatct ttgaagacct
     2341 agagccaaac tactaccact ttatggatgc cgatggcaca cctcgaacta gctttggtgg
     2401 tggacgcttg gggacaaccc accatatgac caaacgcctc ctagttgact ctatcaaata
     2461 cctagttgat acctacaaag tggatggatt ccgtttcgat atgatgggag accatgacgc
     2521 cgcttctatc gaagaagctt acaaggctgc acgcgccctc aatccaaatc taatcatgtt
     2581 aggcgaaggt tggagaacct atgccggtga tgaaaacatg cctactaaag ctgctgacca
     2641 agattggata aaacataccg atactgtcgc tgtcttttca gatgacatcc gtaacaacct
     2701 caaatctggt tatccaaacg aaggtcaacc tgcctttatc acaggtggca agcgtgatgt
     2761 taacactatc tttaaaaatc tcattgctca gccaactaac tttgaagctg acagccctgg
     2821 agatgtcatc caatacatcg cagcccatga taacttgacc ctctttgaca tcattgccca
     2881 gtctatcaaa aaagacccaa gcaaggctga gaactatgct gaaatccacc gtcgtttgag
     2941 acttggaaat ctcatggtct tgacagctca aggaactccg tttatccact ctggccagga
     3001 atatggacgt accaaacaat tccgtgaccc agcctacaga actcctgtag cagaggataa
     3061 ggttccaaac aaatctcact tgttgcgtga taaggacggc aacccatttg actatcctta
     3121 cttcatccat gactcttacg attctagtga tgcagtcaac aagtttgact ggactaaggc
     3181 tacagatggt aaagcttatc ctgaaaatgt caagagccgt gactatatga aaggtttgat
     3241 tgcccttcgt caatctacag atgccttccg acttaagagt ctccaagata tcaaagaccg
     3301 tgtccacctc atcactgtcc caggccaaaa tggtgtggaa aaagaggatg tagtgattgg
     3361 ctaccaaatc actgctccaa acggcgatat ctacgcagtc tttgtcaatg cggatgaaaa
     3421 agctcgcgaa tttaatttgg gaactgcctt tgcacatcta agaaatgcgg aagttttggc
     3481 agatgaaaac caagcaggac cagtcggaat tgccaacccg aaaggacttg aatggactga
     3541 aaaaggcttg aaattgaatg cccttacagc tactgttctt cgagtctctc aaaatggaac
     3601 tagccatgag tcaactgcag aagagaaacc agactcaacc ccttccaagc ctgaacatca
     3661 agacccagct ccagaagcta gacctgattc tactaaacca gatgccaaag tagctgatgc
     3721 ggaaaataaa cctagccaag ctacagctga ttcacaagct gaacaaccag cacaagaagc
     3781 acaagcatca tctgtaaaag aagcggttcg aaacgaatcg gtagaaaact ctagcaagga
     3841 aaatatacct gcaaccccag ataaacaagc tgaacttcca aatacaggaa tcaaaaacga
     3901 aaacaaactc ctatttgcag gaatcagcct ccttgcgctc cttggtctcg gtttcttact
     3961 aaaaaataaa aaagagaact aaactagccc tcctatagaa aaatccccca agcattatag
     4021 ctcgggggat taatttttgt acaatatttg ttgtcctaat aaacttgatt aggatttttt
     4081 attaagcctc tttcatagca aaataagctc gtactttggg tgcaacttgt gttccgaaga
     4141 gttcaatagc tctcagaacc tggtcatgag gcatagaacc aagcggtaga tgaagcatga
     4201 agcggtccaa tcctaaatcc tctatcatgc gaatcaattt ttcggccacc tgatctggat
     4261 tgccaacaaa catggcgcca tttggcccta cctgctccaa atattgctca taacgcaatt
     4321 cctgccagtg cggacggtct ttggaaatag catccaccac ttgcttagtc ggatggaaat
     4381 aatctttcac cgcctgctca ccatcttccg caatccaccc ccaagaatgg gctcccactt
     4441 tcaagtcttt gtcagcatgg cccgcttcgc ttccaatctc acgataagcc tgaatcaact
     4501 ttttaaaata acgtggatta ccaccaataa tagcatatac aatcggtaga ccagcctgag
     4561 caatcttcac tgttgattcg acatgaccac ctgtagctat ccacaagggc aatttgtcct
     4621 gaactggacg aggataaact tctttaccag caatcgtttg agtcaatcga ccttgccagt
     4681 ctaacttggt cttttcattg actaactgaa gcaagtctaa tttctcatca aaaagagagt
     4741 cgtagtcttt caagtcataa ccaaacagag ggaaagattc cgtgaaagat ccccttccag
     4801 ccataatctc cgctcgtcca tttgacaaag catcgatagt ggcatactgt tggaacaaac
     4861 gaatcgggtc catgcttgac agaatgctga ctgcactggt caaacggatt ttcttggtat
     4921 tgactgcccc agcggccagg acaatctctg gggctgatac tgcaaaatcc gcccgatggt
     4981 gctcgccaat cccatatacg tccaaaccaa ccttgtcagc cagctcaatc tctgccacca
     5041 actggcgaat gcgttcagca tgactgtaag tttgtccagt cccttcaagc tccgttattt
     5101 ccccaaatgt tgaaattccc aattctacca ttgtgattct ccttgtctat ctctgtcctt
     5161 caatttgaaa aattattcta acacgaatct tgagtacaag caaccaattt gctcacaaga
     5221 aaaagcctag ataactagac ttttttagct tattctaccg ttactgactt ggcaaggtta
     5281 cgtggtttgt ccacatcgag gccacggtgg agggttgcaa agtaagcgac taattgcgtt
     5341 ggtacgacca ttgaaattgg tgagaggtat ggatgtacgg tcgtaaggac gatatcgtcg
     5401 gtatctttgg ctacattctc ttctgcgata gtgaggactt tggcaccacg ggctgcgacc
     5461 tcttggatat ttccacgagt atgattggca agaactggat ctgacaagag agccaaaaca
     5521 ggcgttcctt cttcaatcaa ggcaatggtt ccgtgcttga gttctcctgc agcaaagcct
     5581 tcacactgga tataagaaat ctctttgagt ttgagacttg cttccatggc tacgtagtaa
     5641 tcttgaccac gtccgatgta aaaggcgtta cgagttgttt caagaagttc acgaaccttg
     5701 gcttcaatgg tttctttctc tgaaagagtt gattcgatag actgagctac gattgacaat
     5761 tcatgaatca ggtcaaaggc ttgcgcttta gcattaccat ttgcttctcc gactgctttt
     5821 gcaaggaagg caagggctgc gatttgcgct gtataggctt tagttgatgc cacggcaatt
     5881 tcaggacctg cgtgaaggag catggtatag ttggcttcac gtgagagggt tgagcctgga
     5941 acatttgtca ctgttaagct tggaattccc atttcattag ccttgaccaa aacttgacga
     6001 ctatccgctg tttcaccaga ttggctgata aagatgaaga gtggtttctt gctgagaagt
     6061 ggcataccgt agccccactc agatgagatg ccaagttcaa ctggtgtatc tgtcaattct
     6121 tccaacattt tcttagaagc aaatcctgca tggtaagatg ttccagctgc aaggatgtag
     6181 atgcggtctg cgtcttgaac agccttaatg atagcaggat caaccactac ttgaccagca
     6241 tcatccgtgt aggcttgaat gagtttacgc ataacagttg gttgctcatc aatttcctta
     6301 agcatgtagt aaggataagt tcccttaccg atatctgaca agtcaagttc cgcagtatag
     6361 ctagcacgtt cacgactgtt accatcatag tcttgaactt ccacgctatc agccttgacg
     6421 attaccaact cttggtcatg aatttccatg tattggttgg tttcacgaat catagccata
     6481 gcatctgagc agaccatatt gtagccttcc ccaagaccaa tcaaaagtgg agatttgttt
     6541 ttcgctacat agatgacatc tggattttca gagtcaatca aggcaaaggc atatgaacca
     6601 cggataatat gaagagcttt tttaaaggct tcaagaactg agagaccgtc ttcttccgca
     6661 aattttccaa tcaaatgtac ggcgatttcc gtatctgttt gccctttgaa gtggtgccca
     6721 gcaaggtatt cttctttaat ttcaaggtag ttttcaatca ccccattgtg aaccaagaca
     6781 aaacgttctg tctcagagcg gtgtgggtga gcattgtctt ccgttggttt cccatgagtt
     6841 gcccaacgag tatgtccgat accagttgtt ccctcaacac cagctgtctt agcagacaat
     6901 tctgcgatac gaccaacagc cttcaccaaa tggttatcag taccatctag gacaaaaatt
     6961 cccgcagaat catagccacg gtattcaagc ttttcaagcc cttgaatcaa aatatcagtt
     7021 gcatttgtgt ttccaacaac accaacaatt ccacacatag tatatacgac acaggcaagc
     7081 tgtgctttct ccttaaaatt ggtatagtct aattcatctt ttatagaatc agcaaaaata
     7141 gtatatactt gtttctttca cttgtcaaga gtaaaaattg gtatagttca aattaagctc
     7201 ctgtaagcaa aaaactctga ccgattggga taatcagtca gagtcctttt taaaatccat
     7261 tattatcgct taattctttg aaccagtggc ctgatttctt cagacgacgt tcttgcgttt
     7321 ccaagtctaa ttcgaccaaa ccatagcgat ttttatagct gttgagccat gaccagcagt
     7381 caataaaggt ccaaatcaag tagcccttac agttggcacc atcttcaatg gcacggtgaa
     7441 gttcacgaag atgacctttt acaaagtcaa tacggtaatc atcttgaatc attccatctt
     7501 gacggaattt ttcttctcct tcaacaccca taccattctc agtcaacatc cactcaatat
     7561 tgccataatt ttccttgata ttttgggcga tgtcataaat tccttgctca taaatctccc
     7621 aaccacggtg agaattgatt ttacgtccag gcatcacata aggctcgtaa aaatgttctg
     7681 gtaagagtgg actatctgga tgcttagcaa atcgaggagc cataacacgc aaaggttgat
     7741 agtagttcac accaaggaag tccaccgtat tatcacgaat gagttccaac tcttcctctg
     7801 tagcatcagg taaaagaccg tgttcatgca agatttctac caactcctgt ggataagtcc
     7861 ccaagacaga tggatctaag aaagattggg cctgaaaaag ggccgcaata cgagctgcct
     7921 tgacatcagc aggatgctgg ctacgtggat aagccggtgt caagttgagg acaatcccaa
     7981 tcttggaatc aggcaaaagt tcatggcaag ccttaacagc ccggctgctg gccaattgtg
     8041 tatgataggc taccttaaca gctgcctctg catccacctt atgtggataa tgggcatcat
     8101 aaaaataacc aaattctaca ggaacgatgg gctcgttaaa ggtaatccat tgatccacta
     8161 aatctccata agtctcaaaa caaaaacgag catagtcttc ataggctgag actgtcgcct
     8221 tattttccca accatcacca tcctcttgaa gggcaaaagg taaatcaaaa tgatagagat
     8281 tgactaacag acgaattcct ttagccttaa tagcctcaaa gaccttacga taaaaatcca
     8341 caccttgagg gttgactttt ccacagcctt gtggaaaaat ccgtgaccac tgaatagaag
     8401 tccgaaaggc tgtgtgacca gtctctaaca aaagctcaat atcccgatcc caattttcat
     8461 aaaaagtcga tgtcttatct gaaccaatcc cattatagta acgatttggc tccacttgga
     8521 accagtaatc ccagagattg tctcccttgc cgtcaccagc tacacgtcct tctgtctgcg
     8581 gtccagaagt agaggatccc cagacaaaat cctttggaaa tcttagcata catttacctc
     8641 tttatctact catttctccc attatacaga aaaaacaagg taaaaactag ttacattttt
     8701 tccttgtttt tcttctgatt atagttttta tttcttgctt aggatttcaa gcgtttcaag
     8761 cacgttatct gcatgaacct caatggtgtc accagttgcc ttgatcttaa cttctacaat
     8821 gccatcggcc gcttttttac caacagtgat acggattgga agaccaatca agtcactatc
     8881 gctaaattta acaccgacac gttcgttacg gtcatctgtc aagacttcat aaccatctcc
     8941 catcaagctt gcttcaagtt tttctatcaa ggcttgcgct tcttcatcct tgacattgac
     9001 agtaatcaaa tgcacatcaa atggtgccaa ttctttaggg aaattgattc cccaagcgta
     9061 acggtattca ccttttggcg ttttgttaac aaagaggcga gcgtgttgct ccatcactgc
     9121 tgaaagaaga cggctgacac cgataccgta acatcccatg atgattggca cagcacgacc
     9181 attttcatcc aagacatctg ctcccatgct tgctgagtag cgagttccga gtttgaaaat
     9241 atgaccaatc tcaataccac gcgcaaagtt aaggacacct tgtccatctg gggaaatttc
     9301 accctcacga acttcacgga tatccacata ttctgcagta aaatcacggc ctgggttcac
     9361 accagtcaag tggtagccat cttcgttagc accgacaact gcattgcgaa catcttgcac
     9421 cttacgatct gcaataattt taatattctc tggcaaacca actggtccaa gtgaaccaaa
     9481 tcctgcttga acaacattcg ccacttcttc ttcgctagca acgtcaaaga aatctgctcc
     9541 taagtggttt ttcaacttga cttcgttgag ttggtcattt ccaactagaa gggctgcaac
     9601 aagctcacca tctgcaatgt agaagagggt tttaatcgtt tgttcttctg gaacattgag
     9661 gaaagctgca acttcatcaa ttgatttaac atctggcgtt gcaacacgag taacttcttc
     9721 ttcagcgaca acacggttgc ttggtttgta ctcgtttgtt gccatttcta agttagctgc
     9781 atagctagac tcacttgagt aagcaatggt atcttcacca gagactatcc atttgagcaa
     9841 ttctgccttg atttcttctt gcacttctgc aggaatttcg tcaaatgagg caactgactt
     9901 gtccaagaca acccagcggt caaggtctgt acgagcagat gtaatggcca taaattcttg
     9961 gctatcctta ccacccatgg ctccaccgtc accaataata gccttgaagt ctaaaccact
    10021 acgagtgaaa atacgctcat aggctgcttt gtactcatca taaacactat ccaaactatc
    10081 atagttagcg tggaaactat aagcatcctt catgataaat tcacgtgtac gaagaagtcc
    10141 attacgtggg cgtttttcat cacgatactt gggctgaatt tgataaaggt tgagtggcaa
    10201 ttgcttgtaa gatttaacag aatcacggac aatagctgta aaggtttcct cgtgagttgg
    10261 acctaagata aagtctgatt tttcacggtt ttttagtttg taaaggtctt caccataggt
    10321 ttcgtaacga cctgattcac gccacaattc tgcactaaga agggctggag ccaacatctc
    10381 aacagcacca atcttttcga attcttggcg catgatgttt ttagcttttt caatcacacg
    10441 gttggcaagt ggtagataag aataaacacc tgctgaaact tggcgaacat aaccagcacg
    10501 caacataaga gcatggctga taacttgagc atcgcttggc atttcgcgaa gcgttgggat
    10561 aggcatttta ctttgtttca taatattcct cgattatcta aaaaagagtc gcataatgtc
    10621 attccaagtc acagcaatca tcaagacaac catgatgacc actccggcca aggtgacata
    10681 ggtttcaatt tcttgtttca atggtttgcg gcggatggct tctaggatat tgagcacaat
    10741 cttaccacca tccaaggctg gaatcggaat aagattaaaa atcccaatat tgatggaaat
    10801 cattgccaag aagtacaaga tattttcaat tccattttta gcagcatcac tacttgcctt
    10861 aaagatagca acaggtccac ccaacttgtt caaatccggt tggaaaatca gatttttcag
    10921 agctgagaga attcggagag ctgagtcagc agcagttgta aaaccaccta caaacatgga
    10981 tagaaaatct gacttaaccc ccggttgaac acctagaagg taacgacctt gactatcttc
    11041 gggtgtaaca gtgacttgtt tgtcactccc cttttcagaa atagtcacat ccaaagtcgg
    11101 tgccgtctta tctttggttt ctgtttccac agcttggatc aagctttccc agttgctaac
    11161 ctcatgtgag ccaatcttgg taatttgtgc cgtttctggt actcctacct tggccaaggc
    11221 accttggggc atgatatgga actgattggt atcaacatct ctgacaccac cctgcataaa
    11281 gattaaaacc caaaaaacaa cgacacctaa gataaaattg ttcatagggc ctgcaaaatt
    11341 ggtaatcagt ttgccccaga tagtcgcatt ttgatattga acatctaaag gtgcaatccg
    11401 aacctcagta ccatctgctt ccacaaccgt tgcatcgtga tccactgcaa atgttttttc
    11461 ttcttccaga accaatcctt tgataaagag cttgtcttca aaatcaaact gggtcacctg
    11521 catagggagg gctgtttgat ccaatttttt acctgagaga ttgatgcgtt taaccttacc
    11581 atcatcagca agtgtcaaac taacaggcgt tcctgtcttg atttcagttg tatcatcacc
    11641 ccaaccggcc atgcggacat agccacccag aggcaagatt cgaatggtat aggccgttcc
    11701 atccttgcca atgtgagcaa aaattttagg tcccataccg atggcaaatt cacgtactaa
    11761 aatccctgat ttcttggcaa agtagaagtg cccgaactcg tgcaccacta caataatccc
    11821 gaaaaccaga ataaaggtta aaattccgag catagcgttt cctccgtctt ttgattaaaa
    11881 gagtccaaat aagtgcatga ttggaaatac aagcaacata ctatcgaaac gatccaaaac
    11941 accaccatgt ccagggataa atttcccaga atccttaaca ccaaaatgac gtttgatcga
    12001 actttctagt aaatcaccaa attgtccagc aatgctaaag aaaatagcaa agactgacat
    12061 cttgtaaatt ccatatggaa gagcaactgt actgtcaact atcataaaga taatggttac
    12121 taaaattgct cctaaaatac cacccaaggc accctcaagg gttttattag gcgataccct
    12181 tggtgctaac tttcgtttcc catagttcat cccaacaaga taggcaccac tgtctgtcgc
    12241 ccagacgata cacaaggcta agagagcctt gtccaaacct gcaacacgag catctagtaa
    12301 agcattaaat ccaaagccca cgtagaagct catagcaaga gggaaaaccg catcctcaat
    12361 cgtataagac ttgctaaaaa cggtcgttcc taacatgatt gaaatcaaaa cactataggc
    12421 aaccacattc ccatcaactg gcaaaaaagt caggtaattc tccaagggaa tggtcaatgc
    12481 aaaggttgca aagagggtca agaggccctc catcgtcatg gtctctagac ctctcatctt
    12541 caaaagttca tgcatggcta gcatggctat gattccgatt gctatctgaa gcaagaggcc
    12601 cccaatcatt aaaattggta ggaaaatagc cagggcaatc cctgcaaaca aggttctttt
    12661 ctgtaaatcc tgggtcatat ttcctcctaa actcctccaa atcggcgatg acgacgatta
    12721 taggcaagaa tagcttcctg caaggccgct tcgtcaaaat caggccataa ggtgtccgta
    12781 aaataaagct cactataggc tccctgccat ggaaggaaat tgctcaaacg caattctcca
    12841 ctagtacgga taatcaagtc tgggtctcgt aagtccttag gcaaatgctg ggtaaagaga
    12901 tagttaccaa tcaattcctc tgtgatgtca cctggattga ttttggcatc taaaacatcc
    12961 tgggaaatca acttaagcgc ctgtgtaatc tcagcacgtc caccatagtt aagagcaaaa
    13021 ttaagaatca atcctgtgtt gttcttagtc aattcctcag ccttggttaa agcttcaaag
    13081 gtttgcttag gcaggcggtc tgtctcccca atcatttgaa tcttaacatt attcgcatgt
    13141 agttccggga cataattatc ataaaactct actggcaagt tcatgataaa cttgacttcc
    13201 tgatctggac gggtccagtt ttccgtagaa aaagcataga ccgtaataac cttgacgccc
    13261 agtttgttgg ctgccttggt cacggtttgc aatgcttcca tgcccgcctt atgtccaaaa
    13321 actcgcggtt gcatacgttt tttagcccaa cggccattgc catccatgat gatgccgata
    13381 tgagcaggaa cctgtgtcgg aacctctact tccacagcct tatctttctt aaaaaatcca
    13441 aacatgatct tattcctatt caaaaatcta tcgttttatt ataccatatt tccccatttt
    13501 cttctatcac taagctattt attctcaggc accaagccca tttttcaaaa aaataagccg
    13561 cctgattggg cgactttatt tttataggga gattattatg aaaaagtttt aggagtttaa
    13621 gttaaggtct tcttaactta tgaacttagt gtacactccc tagcttaaag tttccttaag
    13681 tatttttaaa aatcaaattt ttccatttct cctgccaatt tttcttggat aaacgtgttt
    13741 gatagagttc cattcggtct tcattttcta agaaatgagg agttggacga acttgaaaat
    13801 tcaaaatatc ctccaaacca taaggtgcat agagttcaaa atctgattct tcattcaagc
    13861 gcagtccaac tgtcgtacac cgttctggat acttactcat agcatcacga gaactggtat
    13921 aggaagcagt gtgaggactg tgctgatgca tatagacctg atttttcaat tcccactggt
    13981 actgaggaaa atcctctcgc agctttttct ccagtaataa ggtttcctca taagaaaaat
    14041 ctggatcaaa gaaaatcaca tctatatctg tttcatgatc aaaaggggat ttgtctgaca
    14101 aaagattcca aatgaaattt ctgacagaac ctgctgccaa ccacgagtct ttcagaccaa
    14161 ggtctcggat gatcgtcaga atggccatca tatctggatt ttctctaaaa gcctctaaga
    14221 tttcttgctt atttttcact gtattcataa cctaagtgct catatgcctt agcagtcgcc
    14281 acccgtccag accgtgtccg catgataaaa cctttttgaa tcaagtaagg ctcatacata
    14341 tcttcaactg tctcacgctc ttcggcgata ttcacagaaa gagttcctag accaacaggt
    14401 cctccactgt acatctcaat catggtacga aggatttttt gatccacata gtccaaacct
    14461 tcatggtcaa catccagcat agtcaaagcc ttatcggtaa taatatcatc aattacccca
    14521 ttccccatta tctgggcaaa atcgcgcacg cgcttgagga gacgattggc aatacgaggg
    14581 gtcccacgac tacgtagggc caactcagat gctgcctcat gagtgatttc catctcaaaa
    14641 atatctgccg tccgctcgac aatttctgtc aagtcagcat gggcataata ctccatatgg
    14701 cctgtaatcc caaaacgtgc ccgtagcgga ttggagagca taccagcccg agtcgtcgca
    14761 ccaatcaagg taaaaggtgg taactccaaa tgaacactac gactaccctc accagcccca
    14821 atcataatat ctatgtagaa gtcctccata gcactataaa gcacctcttc cactgacatt
    14881 ggcaaacgat ggatctcatc aataaaaagt acatccccag gctctaactc attcaaaata
    14941 gctaccagat ctccggcttt ttcaatgact ggacccgacg tctgcttaag attgactccc
    15001 agttcgttgg caataacaaa ggccatggtc gttttcccga gacctggagg cccaaataag
    15061 agcacatgat ccagcgcttc atcccgcatt ttggcagctt caataaagat ttgtagctgg
    15121 tccttgacct tatcctgtcc gatatattca cgtaaatact gaggacggag cgtgcgttct
    15181 actaactcct catcccccat tatctcatta tctaaaattc tactcatggc tctattatat
    15241 caaaaaacca agccacaaac aaaaaagcca cctgattggg tgactcctaa gtttagcact
    15301 tatgtggtat aatattctac gacactgcta caccgcctac gaaaggaggt gagatagccc
    15361 atgatggaat tagtactcaa aactatcatc ggaccaattg tggtcggtgt cgttcttcgt
    15421 atagtcgata aatggctaaa caaggacaaa tagtgtcaaa aaagacctca agcttatttg
    15481 gtcgtgagct tggggtcttt tctagcctat gatatagaac tagtactcaa ttccttttta
    15541 ttatcccata gttcacgaat tttgtcaaag gtttacattt tcttcaaccg ctgtacgaca
    15601 agacggttaa gattaagaga acgttaggga ttctatcaat ttcatagaaa ttttgatttc
    15661 gtaaacgaag agacaatctt acatgtcact tctcatttaa tacgccacta ctagacaagc
    15721 aaaatcatta ttacagtagt tccagttctt caattaacag tcacttacaa tcaaattgag
    15781 tttgaactag ctgaagcgac cacagaccta tttcttagtt atattcgcta aaaaagtccc
    15841 cgccaattcc ccgaccaaaa tccgaaaaat accgaaaaat atcgaaaaat tatttttaga
    15901 atagtcccca aaagcctgaa atagagctaa aaaactccac ctgattgggt ggagttaagg
    15961 gagattatta tgaaaaagaa aagtttagga ttttattaaa taaagttagg aggtctttat
    16021 ttaatgacta tataatacta gaccatcctt aaattttgct taagaaaaaa caagttttgt
    16081 tattttttac gattcatcca agtcatccct atacaattat aggtggataa gatttcaaag
    16141 cccatagaac gatagaagcc caaggttctt tctgtctctt ctgtcaccag ctggacttga
    16201 taggcatctt tgtaatcctc taaagcctct ttcatcaagg cactaccaat tccttgacgc
    16261 tgatagattg gtaaaacgat taaatcctga accaatactg atgagaatcc atctccaacc
    16321 aaacgaatca agcccaccac agcatcacca tcaagtgcca gataaattac taatgaatga
    16381 gataaggcct gctccagcat ctctggttga tgggtataat ttgtccaacc gacagcctga
    16441 tagagatgta aaacatcctc tagcttgaca atttcttgct ttttaatagt aatcatctca
    16501 acacctctta aagttctctc aagctcttgt actgctgtcc atttttatca aaattttcag
    16561 gacgcaacca tgtttccaaa cgatctttga cttgaggcca gtccttatca atcatagaca
    16621 accaatccgt atctctcgta cgtcccttat aaaccactgc ctgacggaag gttccttcat
    16681 aaataaagct caaacgctcc gcagctcgtc tggatggcag atttaaggca tcgcatttcc
    16741 actcgtagcg acgatagttt agctcctcaa aaacatagcg agctaggaga tactgagctt
    16801 cagttcctat ccgtgtaccc cttagctctg gagaaaaagt gactgctccg acttctatta
    16861 ctcggttatt ctggtcaatg cgcatgagag aaaaagatcc caaagcctta ccagtcgcct
    16921 tgtctatgat tgcatagtaa aaacggtcct tacgagccaa catctgatgt aaaaggctaa
    16981 ccagctcccc catatctgct actggctcct gaaaaaggta ggtccacatc tcctgaggcg
    17041 tatcaggccc ataaacagct agcaaatcct ccgcatgctt ttccactgaa agagcctcta
    17101 tcctagcata acgcccttct aagaaatcaa tagaaggcag ttcacctggt gtataacctt
    17161 ccattgactc cccaatcatt tgaccatatt catttactgg catagctttc tccttttttc
    17221 acttttcaaa ctttatagtt gcccatagta taccatactt tcataagaag attcaaaaaa
    17281 tcctccagat tctactctga aggattccta ccttatttca caacgatatt aacgagttta
    17341 gttggctcaa acagtttggg aaactgtttg aggttggaaa tcagcaaata aattggttat
    17401 tttacaacaa tattaaccaa tttatttggt acactaatca ctttcacgat ttccttaccg
    17461 tcaatttctg ctttgacttt ttcatccgct agagcaattt cttgcaattc ttcacgtgat
    17521 aggtctttag cgaccatgag tttggcacgg actttacctt tgatttggac aacgatttcg
    17581 atttcgtctt caaccagttt actttcgtcc catgttggcc aagtcacgta agagattgac
    17641 tcacctgttg cagcaacagt ttgccagagt tcttctgcca agtgaggtgc aaatggggca
    17701 atcaattgga taaatccttt ggcatagtca acatagagct tgtcttcctt gttagccgca
    17761 ttgacaaaga ccataagttg ggcaatggct gtgttgaatt tgagggaatc aatttgctct
    17821 gtgacagctt taacagtttc gttgtaaacc ttgtcaagag cgccattgtt ttccgcaaca
    17881 atttccttac ttctaatcaa acggtaaaca cggtcaagga acttacgact tccttccaga
    17941 ccttcttctg accaagcaat cgaagcatcg agtggtccca tgaacatttc atagacacga
    18001 agggtatcag caccgtattg ttccaccaca tcgtctgggt tgacaacgtt cttgagcgat
    18061 ttagacatct tggctggcgc ttgctccaac tcttcccctg tttccacatg gaagaaagaa
    18121 ccatcacgtt tttcaacctt gtcagtcgcc acaagagccc cacggtggtc acgatagctt
    18181 gttcccaaaa tcattccttg gttaaagagt ttttggaatg gttccttagt tggaacaaca
    18241 ccgaggtcat agaggaattt atgccagaaa cgagcgtaaa gcaagtgaag tacagcatgt
    18301 tccgcaccac ccacgtagat atctactggc aaccattgtt tgagcagatc ctcgtcagcc
    18361 aatttctcag tattgtgtgg gtcaatatag cggaggtagt accagcttga acctgcccat
    18421 tgtggcatag tatttgtttc acgacgacct ttgacaccat cttcacgagt cacttcaagc
    18481 cagtctgtca agttagctag tggactttca ccagtacctg agggacggat atccttggtt
    18541 acaggcaaaa caagtggcaa ttcactttca gggacagctg ttgaagttcc atcttcccaa
    18601 tgaatgattg ggattggctc accccagtaa cgttgacggc taaagagcca gtcgcggaga
    18661 cggtaggtta ccttctcttg tccacagcct ttttcttcca accaagccac aatcttggca
    18721 atagcgtctt ctttgttgag tccatctagg aagtctgaat taacgtgaag gccatcttct
    18781 gtgtaggcag cttcttcaac atttccacct tcaagtactt cgacgattgg aaggtcaaat
    18841 tgtttggcaa attcccagtc acgttgatcg tgggcaggca cagccattac cgcacctgta
    18901 ccatagctag caagaacgta atccgcaatc cagattggaa tttccttacc attgacagga
    18961 ttgatggcat aagcaccagt ccatacacca gttttttctt tggcaaggtc ggtacgagcc
    19021 aagtctgact taaggctggc ttggtgtttg tagtctgcta ctgcttctgc ttgctctgtg
    19081 cttgtgatgg catctactaa ttcatgttca ggagccaaaa cagtgaaagt cgcaccgaaa
    19141 agtgtgtccg gacgagtagt aaagactgta aattccttgt ctgttccttt tactttgaaa
    19201 gttacattgg caccagttga tttaccaatc cagttgcgtt gcatatcctt gatagactct
    19261 gaccaatcta gttcatctaa gtcattgagc aagcgctctg cgtaagccgt gattttgagc
    19321 atccattggc gcattggttt gcggacaact ggatagcctc cacgttcaga agttccgtca
    19381 ggaagcactt cttcattggc aatggcagtt cccaattcct caacccagtt tactggcact
    19441 tcagcttcat aggccaagcc tttttcgtaa agcttggtga aaatccattg agtccacttg
    19501 tagtagtttg gatctgttgt gttgacttca cgatcccagt cataagaaaa tccaagcgca
    19561 ttgatttgac gtttgaagtt ggcaatgttt tccgctgtaa attctgctgg gtcattacca
    19621 gtatccatag cgtattgctc tgcaggcaaa ccaaaagcat cccaacccat tgggtgaagg
    19681 acattgtagc cttgcgcacg tttgtaacga ctgaggatat cggttgcagt ataaccttct
    19741 gggtgtccta cgtgcagacc agctccagac ggataaggga acatatcaag cgcataaaac
    19801 ttaggttttg atgcatctgt tcctgtctta aatgtatgat gttctgccca gtagccctgc
    19861 cacttaggct caatttcttt atgattgtaa aaactcatgg tctctctcca attttgtgat
    19921 gttactatta taccattttt gagggaattt gaaatgtgtt taaaggaaaa actgtatact
    19981 gtatctcatt aattttgatg actatgaatt gctctatctc ccacttttta tcccattgtt
    20041 atgcagtaaa tcaaggagta agtcaagaaa cttatctcgt atagcacaaa atacttattt
    20101 tagaccgaag ataaaactgg aatgctaatt aatgtattca caataaatag ccgacaacct
    20161 atgtcgcttt ttgatttatg aagaacgtat agtggactga atctgaaata gtacgaaaca
    20221 attgctaaaa tacttataga aattaatttt actttcccaa tcgatttgtt ctcatcttat
    20281 ttcaatctgc tatagaattc aatacgagga tcaataaaag actaccattt ggaaaacagt
    20341 agtccttatt gtttattttg aatttacata ggcatctaca gcgataatag cttgagcaac
    20401 atctgaaggc gaaatcttaa acggcatctg atgaattgtc tcaccctcca tagttgcttg
    20461 tttaccaact tttattaaat catcatatcc aacttgatcc aaatgcattt cttttagagt
    20521 tgttggcata ccaatttttt tgtaaaactc aatatactta tcaagttctt ctttaggtct
    20581 attttccaat aatagttgta ctaaagttcc ataagctact ttttcaccat gtgttaaatg
    20641 atgaatgtca cctgtcaatg cagtaaaacc attatgaatt gcatgcgccg cagctaatcc
    20701 tccactttca aaacctaggc cactcaataa agtattagct tcaacaatat tttctaatgc
    20761 tggtgtcacc actttagctt cacaagctgc catagcctgt aaaccatctg caaacagcgt
    20821 ttcttcacat ttcttcgcaa ttgcaactcc agccaatgtt tgctgttgtc ccaacatagt
    20881 ttttccattt gcctgcataa ccgcacgcgc ctcaacccaa gttgctaaac catctgcaat
    20941 accagacgct aataaacgct taggggcttg tgaaataact tttgtatcaa ccaaaactaa
    21001 atctggattt ttagaataaa atagataatg atcaaatgca ccttcatctg tataaataac
    21061 agataaagca gatacaggtg cgtcggtcga tgcaattgtt ggagcaataa taacaggctt
    21121 ttcaatcaaa tctgcaatag cttttgcgct atcaatcgtc tttcccccac caagaccgat
    21181 aatactatca caattttctt tctcagccaa ggcaacaact cgattgattt cattgtcaga
    21241 agcttcacca ttaaatagcg ccagaacaat atggaaacca tacctatgta gataatcttc
    21301 aaatcgtttt ccaacaatat cataaaccaa ctgatcgcat aatagaatag ggtaatttcc
    21361 caaatccaaa attgatttgg cattttcaaa caaggcattt tccccctgaa tatatctaga
    21421 aggactagca aaaattctca tatgtatttc ctctctatct aaatggaacg actattttga
    21481 gtaacaaacc aatcgtcaga aaaatcatca accgcctttt ggatagatgg catggcgaaa
    21541 gctgattcaa aaacatctgc tcctgctgta acagcatgcg cacctgcagc taaagcatta
    21601 tttacttgtg ctacattttt aaaggatgca gctaaaatct tactaggaga gttctgtcta
    21661 tcaatagcaa gagctaattg acgaatgaca gaatttgaat caatgttcag attttccatt
    21721 ctattataat atggagctag gtaatccgct cctacttcga tagctaataa tccctgaata
    21781 actgtataaa tagctgttgc agtgatatgg tagccctctt tttttagcac ctttattgca
    21841 cgtaatccag ctggagtaac aggtactttg ataaatatat catctcctgc ttgtcttcga
    21901 atttcatgag catcctttaa gatgccttca aaatcttgag aaatcacctg aacatgaata
    21961 gagggtgtag agccaatcaa ttctcttaca tctttgattc gttcaaaaaa attaatagaa
    22021 ccctctcttt ttgcaatagt gggatttgaa gttaccccag ctagcggcaa aatttcagac
    22081 cactttttaa tctcatctaa atttaatgtg tcaagcataa attccataaa aagaacctct
    22141 ttattttaca aagtatgctc agtacgtcct ataatgtcat cttgggttgc cttagaaaga
    22201 acattgaaga atgcagagta tcctgcaaca cgaacaatta agtctctgtg tttttcagga
    22261 tgtttctgag cgtcaatcaa cgtctctctg gaaacaacat tgtattgaat atggtaccca
    22321 tgtaaacgat taaagaatgt tcgtaacaaa gcaattagtt ttaatttatc ttcttctttg
    22381 gctaacgttt gaggatttac tttctgattt aagagaaccc cacctacgat ttcatctgtt
    22441 ggtaattttg aaacagattt taaaacagat gtagggccgt gttgatccat attatgtgat
    22501 ggtgaacaac cctctgctaa cggtgtaccc gcgttgcgtc catctggagt tgctaatgtt
    22561 ccacgtccct gccctacgtt ggctgagata gaagatgttc ctgaataacg aattcctcca
    22621 ataggccctc ttccataacg tgtattagga tatttagcaa tttcatcaac ataaatgtca
    22681 taagcagcag taaccaattt gtcagcataa tcatcatcat taccatactt aggtgcatca
    22741 tgaatcaaca tttcttgaat gaccttacct tcttctccgg cataatctgt ttccagtgca
    22801 tgccaaagct gacttgggct tatacgttct tcctcaaaca ccaatttttt aattgcagct
    22861 aatgaatccg acaaatttgc aattccaact tgcaatcctg atatataatc atatactgct
    22921 ccaccttctt taaggtgttt tccacgacca atacaatcat cagtcaatgc tgaacataga
    22981 atatcaggaa cttctcgttc caatgataaa tcaatagaat tttcaacaat aacactcatt
    23041 cgtgtcaaat atcttagtgt tttatcccaa gcattttcta attcagaaaa gttcttcata
    23101 tccttaaaat gaccaaagct tggtgcaaac cgtttacccg aagccggatc aattccatca
    23161 ttcatcgtga taagtagaac cttagggaag ttcatataac tcatacctgt gcaacgatag
    23221 ccccatttcc ctggaactgc cgtttcaaca catccaatgg cactgtaatc ataagcatca
    23281 tcttccaata ctccttttgc aataaaagaa ggaataataa tctcatcatt attaaatgca
    23341 ggcataccaa aaccaagttt catcacttca atacactcat tcatgaaacg agcatctaaa
    23401 cctgcatggt aacgtacagt tagattaggt tgcggtagat gggtttgtgc aactgatttt
    23461 aataccaaat aagataatgg gttaacagca tccttcttat ctcgagtctg tccaccaatt
    23521 gtaacatttt gatataaagg acttcctgct gaagaaaatg tatgtgattg actgcgaacc
    23581 ttattgattg taattgtctt aatccaaaga tttgtcagac gttcaacaat gctatcttct
    23641 gtttctttac cactttctaa atcagccttc atatatggat acatatattg atcaaaacgg
    23701 ccatatgaaa gagagtggcc attagattca atttgtaaaa tacattgaat aaaccaaact
    23761 gattgaatag cttctgcaaa agtagttgcc ggttcatatg ggactctaga gcaaatatct
    23821 gcaatctcaa gtaattcttt cttacgttta ggatttgcat tttcggctaa acttttagca
    23881 agagcaacaa agcgctttgc atatacttta atagcatcga ttacgataaa tatagagtcg
    23941 taaaaatgat atttatcaat acttgctgga tctgttaaat ctagagctac ttttgcttta
    24001 cgagcccgct cttcaaaacc tcttaaacca aattgcaaaa gtttctgata gttaactgct
    24061 aggtgagcat ctccagaatt catcttacct tccataccga agaatcctgt ttccatataa
    24121 acagacactt cttcaggtaa taaggcacca gctctagcac gtaaattatt attttcccaa
    24181 aacggagcaa tacttctaag ttgttctttt gtttcttctg taatatagaa aacatctcca
    24241 tcacgctttt caaaaagatc caactcattg agaacaaatt ctagcgtata ttccggaaaa
    24301 ataggagcat ctttattgga agaagcttga tttcccgcaa tcatagattc ttcttcaata
    24361 tagatagtca tattttccaa aatttctttc agcatatatg cacgttttag gacattaggt
    24421 tgttccttgt atcgttcata ggcgcgtgtt gctagaactg ctctctcagc atcaatataa
    24481 ggttttttat ttaaaacatc ttctcgatat ttgttcatcc tttcagtaag gctgccaaaa
    24541 tattctgtct tgattgttgt tcttgctact tctgtattta ccatcgtaac cacctttctt
    24601 tcgaaaatat ttttatttct ttcgaaaata taataccgat ttttatagaa attttcaagc
    24661 ttttaactag aaaaatttta actctctaat ttttagcaca aatgtgtgag tttaacagta
    24721 acacacaaat acctctaaga ttgtttgatt tcattttggt aagctaaacg atcctgtact
    24781 ttaaagaatg gaaaataaac caatgtagac atcgctaata tcaccagctg agtaataact
    24841 ccttgccatc cacccaccaa aaatcctgat aaaatagctg gtgtactcca aggcaatgtt
    24901 acccctgaga atggctgcat gaaacctgtt gcaatagctc catatactat cacagctgca
    24961 agtacaggaa caagaatgaa aggtacaaac ataactggat tcatgacaat cggaaatcca
    25021 aatacaactg gctcatttac gttaaatatt gctggaaaag ctgcaacttt tcctaaggtt
    25081 tggtattgtt ttgattttgc tgcaaaaagc atggcaacta caagaccaaa cgtaatccct
    25141 gaacctgata gaattaaaaa tgaatctaaa aattgttgag taacaatatg tgcaccattt
    25201 tctaatgata gattagcaga ggctaacata gctttattag cgtcaagatt agataaaagc
    25261 agagctgtca ctactccatt tactaccgat tgcccatgaa caccaaacca ccacaaaaat
    25321 gatataaaga atgcaattcc aatagcacca tacaaagatc cagttaaacc ttgcaacgga
    25381 acttgaatag cagaataaat catttctatg aatgttccgc cattagtcaa tgacttcgct
    25441 aaaatatata caatcataga agataagaaa attacaaatg ctggaatcat tgcttcaaac
    25501 tgtttggcaa tagcttgtgg aacttgttct ggcatcttaa taacaatttt tctctttata
    25561 aagaaggtat aaatacttcc tactaccaaa cctataatga tagcaccgat aattccttgg
    25621 cctccaaacc aaactttact aatagcgtcc ccaatcgcct caccttgttt agggatataa
    25681 gatgatctta gcaaaataaa gaatgcagat acagatagaa ctccagctgg taaagcctct
    25741 actccgctat tcttagcata agaataggca attgaaaaac aagaaattag acccataata
    25801 gcaaaagttc ctgaatatac ttgcataaac ggctctgtcc aattagctcc aaaaacacta
    25861 gcaatgctct tatttaatcc ttcgaacggc aattgtccca taatcaagaa caaactacca
    25921 actactgtca atggcaaaat tgctaacatc ccatctttta gagctataat gccacgcata
    25981 ttcacaaact tcatcatcgg tgcaatgatt ttctgaacat ccatctttga cataataaat
    26041 ctccttttct tacccactaa tcaaagatag ggccaaatct aatacttttt tcccatctaa
    26101 cataccatag tccatcatcg gaataacagc tatcggaaca tcacacttat cacaaatttc
    26161 ttttgattta tctaatgtat aagcaacttg tggacccaat agtgcaacat ctatatttgg
    26221 cgcataatcc gctaatttag actgagaaaa cgcctctatt tctgcctcaa ctccactagc
    26281 ttgcgctgca attttcatat tatttacaag cataccagta gaaaaacctg ctgcacaaaa
    26341 caaaccaatc ttcaccatta tgttttcctc ctctatgtta ataacaatga taatactcta
    26401 gtattatttt ttatgaagtt cttttctcaa actaataatt tctttgatta aattaatctc
    26461 cgtcatacta gtcatgagat gatcttgtga atgaacaaag agagctgtaa tttctgtctt
    26521 tgtaccagac gcttcctgtg ccaagaattt tgtttgtaga ttatgagctt ctaataaggc
    26581 ggcatcagct aactggattt cttcttcaca tcgttcacat gtaccgttct ttatgtaatc
    26641 taatgcttta taaatatgtt gtttcgcatc gccagcatat aaaattaaac ccatgataat
    26701 ttgatctgga acaataattt ccataaattc ctcctcattt cactatgtaa aaagcttttt
    26761 tcatttgaaa ttttaaaatt ttcttttgta actatagaat acaactacat aataaagttg
    26821 tcaatccaaa aacaacagat aaactatttt tgtttatata agcacaaatg tgcaatcgac
    26881 taaaaagaaa aaacgataaa ctgatcagtc tatcattttc actcacctag aatctctcta
    26941 aaagtcaaat caccatcttc ttccaaaact tttaaaattg tatttttatc tgtaatcaaa
    27001 tgatttacta aattagcacg caagacagaa agaatcgaag atactttcgt atcaccgtaa
    27061 gcaacagcta aactttgagg aatatttttt aaatcttcca aagagatggc tatcgttctt
    27121 tcctgtaagt tttcatagac ttctttacct tttgaatcaa aaaatcgaca acaaatttct
    27181 cctacagttt taactttggt caattcttta aagtcatcct ctgtaagcat gtctaaccat
    27241 tgatgtttcc ctttattgct aaaatcacca attccgacta cagctatatc taaatctttc
    27301 caactatttt tcaaattttc aaaatatctt gattgcaaaa taccatctgc taacaattta
    27361 ttttcttgca caatcgttgc attcataaat gtacactctc catgaaattt tctagacatt
    27421 tcataaatca gtgtattcac atggtattta gcgtgtatgt gactaggacc acctgctaga
    27481 ggatagaagt gaacatttcg gacactttta ctgtgaatta aatctactaa attacttaaa
    27541 cttttccccc aagaaaagcc aattttcata ttatcatcaa ttagattcct aaggacgcct
    27601 gctgcaactt gagaaattct ttcagataaa attgttggag tatcatcaaa ttcatttgga
    27661 ataatttcta aactttccaa actgtatttt tcttttacat aattttccaa cttaaacata
    27721 ttggtatcaa aattctctat ttcaatttta acaattccta cattccttgc ttctgttaac
    27781 attctactaa tagaggttct ataaattcct aattttgctg ctatttgtga ctgatttaag
    27841 ttttcaatat aatacagata agcaatttta gaaagcagtt tattcctatc ttgattcata
    27901 cacttaacct cttacgaaac taccttaacc attatcccag cattttctaa tgtagctata
    27961 ttttgtttag aaagtttttc gtctgttatt acttcataga cttgacttaa agcaaatctt
    28021 cttactgtac ctcttttatc aaatttactt gagtcagtta ggacaatgac tttatccgac
    28081 actgctgaaa tatattgaac tacctcactg cgcattaaat cttttccggt aaagcccatc
    28141 tctttatcgt aaccatctgt cccaacaaaa gcttgacaca catgaaaagt ctgtatcatt
    28201 tcttttaata aaggtcctac agtcacctgt gaatctttct gaaactcacc accaagaaca
    28261 ataacacgac atgaatcata agctctcaca aaatttgcta taaaaaacga atttgttaca
    28321 atcgtaacat ttcttttttg cttgcaaatt tcctcagcaa gtaaagcaca ggtcgatcca
    28381 gattctatca ttattgtttc attatctgac accaatttta ctgcttcctg aacaattttt
    28441 ctcttagttt cataattaat tgacaaacgt acatttaagt catctccact atttaataca
    28501 gcatatccat gctctctgtg taataaacct tttgactcta atttatctaa atcttttcta
    28561 atcgttactt tcgatacatt taatttttcc gataatgtat taacgtcgat cttttcatat
    28621 tctgatacta atttaataat ttgttccaat cttttcattt tacacctccg ttttattcta
    28681 ccaaaataaa aagcaaaaaa caacaaatta aactttcgtt cgtaattgtt tttctttcgt
    28741 ttttgtgata ggatagactt atgaagagga ggaactctta tggaaatatc taaaggaatt
    28801 atttttaata ttcaacactt ttcaattcat gacggcccgg gtattcgtac aactgttttt
    28861 ttaaaaggat gtcctctgcg ctgtccatgg tgttctaacc ctgaatctca aagaatgaaa
    28921 cctgaaaaaa tgaaagatgc tcaacgagag aaattcacct tagtcggtga agaaaagact
    28981 gtagaagaaa ttattacaga ggtattaaaa gacaaagaat tttacgaaga atccggtgga
    29041 ggtttaactt tatcaggagg tgaaatattt gctcagtttg aatttgctaa agccatctta
    29101 aaatcagcta aagaacatca catacacact gccattgaaa ctactgcctt tgttgatcat
    29161 gaaaaattta tcgatttaat tcaatatgtg gattttatct acacagacct aaaacattat
    29221 aattctataa aacataaaaa agtgactggg gtttttaatc aaatgattat taaaaacatt
    29281 cattatgctt tttctcaaaa taaaactatc gttttaagaa tcccagttat tcctaatttt
    29341 aacaatagtt tagaggatgc agaaaaattc gctactctat ttaactcatt aaatatcgac
    29401 caagttcaac tactcccttt tcatcaattt ggtgaaaaca aatatcgttt attaaatcgg
    29461 aaatatgaaa tggatggagt caacgcactt catcctgaag atcttattga ttatcaaaag
    29521 gtatttctga accaccatat taattgttat ttctagttca tttccttgaa acgctctagc
    29581 tatttgcaga taacaagcat ctatataagg ttatcaaaga agacttcccc atgactctaa
    29641 atgaatatat cttacaatat cgcctgaaac aagctataga taagatggct gaatctccca
    29701 actcctcttt aagcgatatc tctgaccaag ttggattttc agactataaa tattttgcca
    29761 aagtatttaa aaagtatctc catatttccc caaaaaaatt gaaatcactc ggaagaatag
    29821 taaaataggc tattcttctt tttgtagaga attttactct aatatagtga attgaaataa
    29881 gatgtgaaca tctctatcag gaaagtcaaa ttaatttata gaaatatttt agcagtcaag
    29941 gtgtactatt atagattcaa tgcactatag aatagaattg caggttttaa aaacgcttac
    30001 attgttttaa aataaactta aataacacaa cggaggtatc catcatgaaa aagaaatgga
    30061 tgtattatgc tgcttgttct tctaatgaat ctgccgatga cagttcatct gataaaggag
    30121 acggtggttc gctagtcgtt tattgaccaa actcagaagg cttaattgga gcaactattc
    30181 ctgcctttga agaaaaatat ggtatcaaaa tagaactgat tcaagctggt actggagaac
    30241 ttttcaaaaa actagagtca gaaaaagaag ttcctgtagc tgatgttatc tttggtggtt
    30301 cttatacaca atatgctacc cacggagaac tctttgaaaa ctatatttca aaagaaaatg
    30361 ataatgttat caaagaatat caaaacacaa ctggcttctc tactccttat acactagatg
    30421 gtagtgtttt aatcgtccac cctgatttaa ctaaaggcat gaacatcgaa ggatatagcg
    30481 accttctcaa acctgaacta aaaggaaaaa tcgcaactgc tgacccagca aactcttcta
    30541 gcgcctttgc tcaattaaca aatatgctac aagctcaagg tggttacaaa gatgataagg
    30601 cttggtctta tgtaaaagat cttttcacac ttattgatgg tatagtaaaa tgaaataaga
    30661 acaggacaag ttaatcagga cagtcaaatc gatttctaac aatgttttag aagtagatgt
    30721 gtactattct agtttcaata tactataaaa tcggtttaag ttcatctggt gtctataaag
    30781 cagtcgctga tggagaaatg actgttggtc tctcttatga agatccagca gttaaactct
    30841 taaatgacgg agctaacatt aaggtagtct atccaaaaga aggaaccgtc ttcctacctg
    30901 ctagtgctgc tatcgttaaa aaatctaaaa atatggaaaa tgccaagaaa tttatcgatt
    30961 ttattatctc tcaagaagta caagatacac ttggtacaac cactactaac cgtcctgttc
    31021 gtaaaaatgc taaaacaagc gaaaacatga aaccaattga caaaatcaaa acactcactg
    31081 aagattatga ttatgtcatc aagaataaat cagatatcgt taagaaatac aacgaagtct
    31141 ttacagatat ccaatctaaa cagtaaaaga ggttcactat gagtgagatc aaaattatta
    31201 acgccaaaaa aatctaccac gatgtccctg ttattgagaa tttgaacatt acaattccaa
    31261 aagaaagtct ctttaccctt cttggacctt caggatgtgg gaaaacaacc cttcttcgta
    31321 tgattgcagg tttcaacagt atcgaaggtg gagaatttta cttcgatgat acaaaaatca
    31381 ataatatgga acccagcaaa cgcaatatcg ggatggtttt ccaaaactac gctattttcc
    31441 cacatttgac tgtccgagac aacgttgctt ttggtcttat gcaaaagaag gttccaaaag
    31501 aagaattgat tcaacagacc aacaagtatc ttgaactcat gcaaattgct caatatgcgg
    31561 atcgaaagcc cgataaactc agtggtggac aacaacaacg tgtcaccttg gcatgcgcct
    31621 tagcggttaa tccaagtgtt ctcctcatgg acgagccact tagtaatctg gaggccaaac
    31681 ttcgcttgga tatacgtcaa gccatccgag aaatccaaca cgaagtggga attacaactg
    31741 tttatgtaac ccacgaccaa gaagaagcca tggctatttc agaccaaatt gctgttatga
    31801 aagatggggt gatccaacaa atcggccgac caaaagaact ctatcataaa ccagctaatg
    31861 agtttgtggc aacctttatc ggacgcacaa atattatccc tgccaatctt gaaaaacgga
    31921 gcgacggcgc ttatatcgtc ttttcagatg gctatgccct tcgaatgcta gctcttgatc
    31981 aggttgagga gcaagctatt catgtaagca ttcgtcccga agagtttatc aaagatgaat
    32041 ctggagatat tgaaggaact attagcgata gcgtctatct tggactaaat acggagtatt
    32101 tcattgagac aggttttgcc tcaaaaattc aagttagtga agaatcaact tttgaagaag
    32161 aactacaaaa aggcaatcgt attcgtctac gaatcaatac gcaaaaatta aacatctttt
    32221 ctgcagatgg ttcccaaaac ctgataaaag gagtcaacca tggaacgtaa aaaactaaat
    32281 atttggacag cctcctcttt cttcatcttt cttacctatc ttgtctttct cgtttatcct
    32341 atcgttaccg tgctcaagta agcacttata catgaaggat aattctcact agctaatttt
    32401 gtcactttct ttagtaaaac ctactactct gagacactag tcaacagttt cagggtttcc
    32461 attaccgcta ctgtcacttc cttagttgta ggaaccctat tagcttatct cttctctatg
    32521 tatgacttca aggggaagaa atttctacaa atattgatta tcattgcttc catgtcagct
    32581 cctttcgtag gagcctactc ctggattctc ttgctgagac gaaatgaggt catcactaaa
    32641 tttttgacaa atgcccttca tcttccagct atcgatattt atggattcaa aggaattata
    32701 cttgtcttta cactgcaact attcccactg gtatttctat atgttgctgg aacaatgaac
    32761 agtattgaca attctctact tgaagctgct gaaagcatgg gatccttcgg atttaaacct
    32821 atcgtaacgg ttgttttacc tctcctagtt ccaaccttac tagcagcccc ttgcttgtat
    32881 ttatgagagc attctcagac tttggaacgc ctatgttgat tggcgaagga tatcggactt
    32941 tccctgtcct gatttatacc caatttatta gcgaggttgg aggaaattct gcttttgcaa
    33001 ttatggcgat tatcattgcc ttggcaattt tccttatcca aaaacacatt gcaaaccgct
    33061 acagtttcag catgaatccg ctccatccaa ttgagcctaa aaaaactaca aaaggaaaaa
    33121 tggctgccat ttatgcaaca gtctacggaa ttatctttat ctctgtttta cctcaaatct
    33181 acttaattta tacctctttc ctaaaaacat caggtatggt atttgttaaa ggttattctc
    33241 caaacagtta caaggtagct ttcaatcgta tgggatctgc tattttcaat accattcgta
    33301 tccctttgat tgccttagtt ctagttgttc tatttgcgac atttatctcc tacctagccg
    33361 ttagaaaacg gaatttattt acaaacttaa ttgacagcct cagtatggta ccttatattg
    33421 taccaggaac cgttctaggg attgccttca tttcttcctt caatactggt ctatttggaa
    33481 gtggatttct tatgattaca gggactgctt tcatcttgat tatgtctcta tctgtcagaa
    33541 gattaccgta tactattcgc tcatctgttg ctagcttaca acaaatagca ccaagtattg
    33601 aagaagctgc tgaaagctta ggaagtagtc gtctcaatac ctttgctaag attacaactc
    33661 caatgatgct atcgggtatt atttctggag ccatcctatc ttgggtcaca ctgatttcag
    33721 aactctctac ttctatcctc tatcctcctc tacaatgtca aaacaagaac aatgactgta
    33781 gctatttata cagaggttct cagaggaaat tacggtgtag ccgcagcctt gtcaactatc
    33841 ctgactgttc taacagtagg ttccttgctc ttgtttatga aaatctctaa aagcaatagc
    33901 attacacttt agttttccac atcaaaaaca gccgaaagga gtaccctcgg ctgttttttc
    33961 ttatcttgat attcttatca aatccctagc tcatggcaag caagtctaga ctgattaaat
    34021 agagccttct tccatcttct cacgtcctag ctctcggtaa ctacggtcac cgacaacttc
    34081 aaccctaaac tgaccgtcct catattcaag aatcgtcacg ctaccattat ctagaccatg
    34141 cggatgcatg ccattaatca gataaacaat ggttccaata gtcattccat ggctgacaac
    34201 aagggcgtta cctccacctt gatcttccat ttcttttgca attatttcaa agccttcctt
    34261 gattcggcca ctgagttttt cccaaccttc agcccaacca gctgtatcga cctctaccaa
    34321 gccctcagcc agttcagcat aagacaattg gtgaacgtgg tccacattaa agatacgagg
    34381 aataatgccc atgaaaagat cgccatcata ggctccatca aaactaccga aacaccattc
    34441 tctgatacgc ttgtccatgc gataagggat tttcccctgc aagccaagtt cttcaagtat
    34501 aattcccatg gtctgaatgg tacgaccaga atcactcgaa taagcgcgct caaactgtag
    34561 atcagattct cgcaaaccga ttcctaactc ttgaatccct cgttcacctt cagcagttaa
    34621 gggagtatcg ctccaacctt gcgcgcgacc aatcgtgtta aacatggtct tgccatgacg
    34681 taccaaatac aatcgtactt ttaccatttt ccgtcctccg tttgcttcta ttatatcatg
    34741 aatgataaaa taaaagccac ccatacaggc gacttttgaa ggagattatt atgaaaaagt
    34801 ttaggagttc attaaataaa gatattagat gaaaatcaaa ttcaaactaa ttcagtgtta
    34861 tctgtttcaa ataatattag gaggtcttta tttaatgatt atagtataca catctaatct
    34921 taaatagaac ttaaaatttc tcctattttc ttaattttta aaactatgaa ttggtgctgg
    34981 gatttgtcca ccgcgagaga tgaagtcgac agacgaagcc ccattaacct tcataacggg
    35041 tgcagttcct aatagaccac caaactcaat catatcgcct tctcttcctt tgggaatgat
    35101 acgaacagct gttgttttca tgttgataac accgattgct gcttcatccg caatcatagc
    35161 cgcaatagtt tcagcaggcg tatcttctgg gatggcaatc atatccaatc caacagaaca
    35221 gatagccgtc atagcttcta gtttttctaa attaagagag ccattttgca ctgcagcaat
    35281 cattccttca tcctcagaaa cagggataaa ggcaccagat agaccaccga cctggttaca
    35341 ggccatcact ccaccctttt taacttggtc gttcaagagg gccaaggcag ctgtcgttcc
    35401 atgcgtgcca actgtttcta gccccatttc ctcaaggaca cgtgccacag agtctccaac
    35461 cgcaggggtt ggtgccaaac tcaagtccac aataccaaac tccacaccca gtctctcact
    35521 ggccatttga ccaaccaatt gaccgatacg agtgatttta aaggcagttt tcttaactgt
    35581 ttcggctact acatcaaagc tctgtccacg aactttttcc aaagcacgtt tcacaacacc
    35641 aggaccagaa actccgacat tgatgataac atctgcttcc ccaacaccat gaaaggcacc
    35701 cgccataaat ggattgtcct caacagcatt agcgaataca accaacttgg ccactcccat
    35761 atctgaaaga tttgctgttt ccttgataat tcgtcccata tctgccacag ccgtcatatt
    35821 aataccagac ttggttgagc cgatattgac tgacgagcag accttatccg tctcagccaa
    35881 agcgcgagga atggaattga tgagaatctc atctcccttt tgataacctt tttgtactaa
    35941 ggcagaaaaa ccaccaataa agtccacacc aatctctttc gcagccttat caagcgcttt
    36001 tgccagaacc acgtagtccg tcgcatctgt cgctgcccca atcagagaaa taggtgtcac
    36061 cgatacacgc ttattaacga taggaattcc caactcagcc gcaatttcat caccaacagc
    36121 tactaaatta gccgcctttg tcgtaatttt ttgatagatt ttctccgcag cacaattgat
    36181 atctggatcg atacagtcca aaagagaaat ccccatggta atggttctaa tatcgaagtt
    36241 ttgctcctca atcatggcga tggtttcagt aacttgtcta atatccatga tgacctccta
    36301 gatattatac atagcttcga aaatcgctgc actctgaata ttgattttta cattcaaagt
    36361 ttgcccaaaa gcttcaaatt cattacgaag ataggtaaaa tcttgctttt catcactaga
    36421 tacaacagcc atcatcgtaa aatattcatc taagacagtt tgagagatat cgtcaatatt
    36481 caatcctaat tctgcaattt taccagaaac acctgcaaca attccagatt tatctttacc
    36541 aacaacagtt ataatggctt tcataagtaa actccaattc ttcttaaaat aagaaacctt
    36601 aacattaaac aggattcttt tcaaatagta tagcataatt ctaacgaaaa tactcaaaaa
    36661 cgcctgctta cgaagttgtt cttaagaaaa aaacaatttc gtaaacaagc gtctactatc
    36721 ccaacttatg atgagtgttc ccgctaggac cgaaatccta gcggtagacc agagctagac
    36781 taagagcaca agactccatc atcataacac tcaacaaaat tgatgatttt atactaattc
    36841 gatgatcgcc attggcgctg catcaccacg acgtggttca gttttaagga tacgagtgta
    36901 tccaccgtta cgttcagcat aacgaggtgc gatttctgag aacaattttt gaagtgctgt
    36961 agtagaagtg tacttatcag ttgcttcatc atagttttca gatgcgattt cattacgtac
    37021 gaaagcagct gcttgacgac gtgcatgcaa atcaccacgt ttacctagag taatcatttt
    37081 ttcaacagtt ttacggattt ctttagcacg agcttcagtt gtcacgattg attcgttgat
    37141 caaaaggtca gttgtcaaat cgcgaagcat tgctttacgt tgtgagctag tgcgtcctag
    37201 tttacggtaa gccatgtatt cctcctttat ttatctttta atccaagacc caaatcaatg
    37261 agtttgagtt tcacttcttc caaactcttg cgtccaagat ttcgtacttt catcatctct
    37321 gcttcagatt tttctgtcaa atcatgcaca gtattgatac cggcacgttt taaacagttg
    37381 tatgaacgca cagacaagtc cagttcctca atcgtacgat ctaaaatacg gtcgtcagat
    37441 tcagtatcag cttctttcat cacttcagtt gacttagcaa tctcagtaag atttgtaaac
    37501 aaatcaagat gttctgtcaa aatacgtgct gaaagcccta aagcatcttc tggaataatt
    37561 gttccatttg tcaagatttc aagggttaat ttgtcgaaac catcattgct acctacacga
    37621 gcaggttcca cttgatagtt gacttttgta actggtgtat aaatagaatc tacagcaagt
    37681 gttccaactg gtgcattatc ctttttattt tcatcagcag gtacatatcc acgaccactg
    37741 ttaacagtca tagtcgcttt tagagaagaa ccttcaccga ttgtaaagag ataatgatct
    37801 ggatttacaa tttcaatatc gctatctgtc aaaatgtcac cagctgttac ttcagcagga
    37861 ccttcaacat ccagttcgat gattttttcg tcttcaacgt acgatttcac tgcaattcct
    37921 ttaatgttca gaatgatttg catcacgtct tcacgaacac ctggaactgt gtcaaactca
    37981 tgtaacacac catcaatgtt gatagatgtc acagctgctc ctggtagaga agctagaagt
    38041 acacgacgaa gagagttacc aagagttgta ccgtagccac gttcaagtgg ttcgattaca
    38101 aacttgccat aatctttatt ttcatcaatt tttgttatat ttggtttttc aaactcgatc
    38161 atttagttac tccctcttaa acgaaaagca gtgtaatgcg atgattatac acggcgacgt
    38221 tttggaggac gagcaccatt gtgtggcact ggagtcacat cacgaattgc tgttacttca
    38281 agaccagcgg cagcaagcgc acgaatagct gactcacgac cagaacctgg accttttaca
    38341 gtaacttcaa ctgatttaag accgtgttct tgtgcagatt tagcagcagc ttcagaagcc
    38401 atttgagcag cgaatggtgt agatttacga gaacctttga aaccaagagc accagctgat
    38461 gaccaagcaa ttgcattacc atgcacatca gtaatcataa caatagtgtt attaaatgta
    38521 gcgtgaatat gagcaatacc agattcgata ttctttttca cacgacgttt acgtgttggt
    38581 ttagccaaga cttttacctc ctatattatt ttttcttacc agcaatcgca acagctttac
    38641 ctttacgagt gcgggcgttg tttttagtgt tttgtccacg gacaggaagt ccacgacggt
    38701 gacggatacc acggtatgaa ccgatttcca tcaaacgttt gatgttcaag tttacttcac
    38761 gacgaaggtc accttcaact ttgattgcat ccacttcacg acggatagca tcttcttgat
    38821 ctgatgtaag atcacgtaca cgaacatctt ctgagattcc agcagcagcc aaaattttct
    38881 tagatgttgc aagtccgata ccataaacat aagtcaatga gattactacg cgtttgtcat
    38941 ttggaatatc aactccagca atacgagcca tgttttctcc tttctatctt atccttgacg
    39001 ttgtttgtgt tttggatttg ctgggcaaat taccataaca cgaccattac gacgaataac
    39061 tttacagtat tcgcaaattg gtttgaccga tggtcttact ttcatttctt atccctccaa
    39121 gtttttcgat tatttaaagc ggtaagtgat acgtccacgt gtcaagtcat atggactcat
    39181 ttcgacagta acacgatctc ccgctaaaat acgaatatag tttttacgaa ttttaccaga
    39241 aactgttgct aaaatctgat gtccattttc aagttcaacc gtaaacattg cattcggcat
    39301 tgtatcaact actttgcctt caacttcaat cacatcgtct tttgccacgc aaaagcacct
    39361 ccataaattt cgattcgatg cctctagaca cagagacaac aattataagt cagactatct
    39421 cagtataaca tttgtagcgg atttttgcaa gtgcgaaaaa cgctttattt caaatttgtc
    39481 aatacttttt caatatctga gaagacatca ttgatatctt gattaccttc gatgtcatga
    39541 accaaacctt tggcacggta gtgagcaatg attggttctc cttgagcaat attaacatcc
    39601 aaacgacgtt ttactgtctc aggcttatca tcttcacgtt ggtagtaatc ttcttcttta
    39661 tagtcaactg gtgggttaaa gaccttgtgg aaagtttctc cagttacgcg gtggatgata
    39721 cggccactca aacgttccaa gaggctgtca gggttcactt caatattgat aataccttct
    39781 agttcaatgc caagttcagc caatgttttg tccaaggcat gagcttgttc aattgtacgt
    39841 gggtaaccat ccaataagaa tcctgtttct ttaatatcat cttgtgaaag gcgttctttt
    39901 acgattccat ttgtaacttc gtcaggaacc aattcaccct tgtcaatata tgacttagca
    39961 agaacaccca tttcagtttg atttgccatt gcagcgcgga acatatcacc tgttgagata
    40021 tgtgcaacat ggaattgttc tacgattttt gctgcttgag ttcccttacc tgcaccaggt
    40081 aagcccataa tcaaaagatt catgatttga tctccttatt ttatttttaa atagaagcaa
    40141 aaagtctttt cacccctatt taaaaacaaa atagaagagg ggagatccaa ctcttacaaa
    40201 tgtcagagtt taaacctcca ctccctcagt atttactgat tcagtaaata cttttattct
    40261 gttctgtcca tgaaaccaac atacttacgt ttcaataggt aaccttccaa ttgcttgatt
    40321 ccttcgatac ctgtagagat aatgatcaag agacttgttc caccaaaggc aacaacatca
    40381 gaaagaccaa atacatcttt agctgcaatc ggtaaaatgg aaatcacacc aaggaagagg
    40441 gaaccaacag ttgcaagacg acgaagaagt ttagacatat attcttctgt acctttacca
    40501 ggacgaactc catggatata ggcaccactc ttttgtaggc tctctgctgc tttttcagga
    40561 ttaatctgta caaacgtata gaagaatgta aagagaataa tcaacaaagc atacatggca
    40621 ataccagttg gagaagtagt tgccaacatc tcttgtgcta cccttaccca agcccaatca
    40681 tgacctgtgg cactcaaaaa ctgaagaata gccgcaggcg ctgcagtaat cgaactggca
    40741 aagataacag ggataactcc agcagggttt acctttaacg gaaggtaaga gctagatgga
    40801 gcaccttgtg caaccttagt atattggatt ggaattttgt attctgcttg ttgaacataa
    40861 gttgtaaagt aaataatcaa caatacagta ataatcaaaa tgattacgaa aatgatagat
    40921 gaagtgatac ggctacttgg gacgttcaca aagtagtcca catagatgcc ctgaatcatc
    40981 tctggaattg aggaaacaat cccggcaaag ataatcatgg aaacaccgtt tccgtatccc
    41041 ttatctgtaa tttgctcacc caaccaagtg acaatcatac taccagctgt taagatgata
    41101 ccaatcgtca gaaaaacttg tggagttaaa gcagttttaa tcaattgagc tccagccaag
    41161 gtattaaaac cagctgtaat cccgatagat tgcacaaaag cgagaactag agcaatataa
    41221 cgagtagctt gattcaattt tcttcgacct acttcccctt gtttacccca ctctacaaac
    41281 ttgggtaaaa tatccatttg caagagttgg acaacaatag aagcggtgat atagggacta
    41341 actcctaggg caaaaatcga aaagtttttt agggcattcc ccgacaccaa gctcaacatg
    41401 tttaagaagg ataatccact taaagcattc aagctattgg cattcacacc aggaactgta
    41461 atgctagttc cgatacgaaa gaccaaaacg ataaaaattg taaataaaat ttttgatcga
    41521 acctgcttga ctttaagagc ttctcttaat aatttaaaaa acataggtca cctctcttag
    41581 atgacttcta ctgaaccacc tttagcagtg atagcttctt cagctgattt agagaattta
    41641 gctgctttca cagtcaattt cttagtcaac tcaccgttac caagaatttt aatacctgac
    41701 ttttcagctt taacaattcc tgcttcgata agaacaactg gagttacttc agcaccatct
    41761 tcaaagacgt tcaattggtc aaggttcaca attgcgtatt ctttagcgtt gatgttagtg
    41821 aatccacgtt ttggaagacg acggaacaat ggagtttgtc caccttcaaa accaaggcga
    41881 actccgccac cgctacgagc tttttgacct ttttgaccac gaccagatgt tttaccgtta
    41941 cctgatgaag taccacgacc aacgcggtta cgtactttac gagaaccttc tgcaggtttc
    42001 aattcatgaa gtttcatttt tattttctcc tcttttgtaa aatgctagcg ccgataaggg
    42061 agaaaaggtt gtctccccca tcaactcgcc tatacgatgt catcatagat gactatatct
    42121 agttttaggg gatggtacag tgcacatccc ctaaaacttc attagtttac ttcttcaact
    42181 gttactaagt gagatactgc tgtaatcata ccacggatag cagcgttatc ttctttaata
    42241 acagagctgt tcaatttgcc aagtccaagt gctacaacag ttttacgttg tgatggaatg
    42301 cgtccgattg gagacttagt caaagtaatt ttaatttgag ccattttatc ccctttctta
    42361 tgccaaatca gaaactgaaa taccacgaag ggcagcaatt tcttcagcgc gtttcaattg
    42421 tttcaaacct tcaacagttg cacgaacaat gttgattgga gtgttagaac caagtgattt
    42481 agatgtaata tctgccacac ctgccaattc cacaacggca cgaactgcac caccagcggc
    42541 aactccagaa ccttctacag caggtttcaa caatacttta gctccaccga attctgaaag
    42601 aacttcgtgt gggattgttg ttccaaccat aggaacttcg atcaagtttt tcttagcatc
    42661 atctactgct ttacggattg cttctggaac ttcttgagct ttaccagtac caaatcctac
    42721 gcgaccattg tggtcaccaa caacaacaag agctgcgaaa cgaagacgac gtccaccttt
    42781 aacaactttt gtaacacggt tgacagcaac tacgcgttct tctaattcaa ctgcattgtc
    42841 tttaaatgcc attttctagt gtcctcctat tagaatttca atccgttttc acgagctgca
    42901 tcagccaaag ctttcacacg tccgtgatat agatatccac cgcggtcgaa caccacttct
    42961 gaaatacctt tagcgtttgc acgttctgca acgagtttac cgacagcaac ggcttgttca
    43021 gttttagttc cttttgaaac ttctttatca agagttgaag cacttgcgag cgttacaccc
    43081 gctacgtcat caatcacttg agcgtagatg cctgtattag aacggaatac gttcaaacgt
    43141 gggcgatcag cagttccaga gagttttccg cgaacgcgac ggtggcgttt ttggcggagt
    43201 ttgtttttat ctggttttga aatcacagtt ttcacctctt tagttttaaa tcgtgtgcta
    43261 tgcacaaagt tggaaaatag gttggtggtt gatgatcaac cactcaacat tatttacctg
    43321 ttttaccttc tttacggcga acgaattcac caacgtaacg gataccttta cctttatatg
    43381 gttctggtga acgaaggcta cgtacgtaag cagctgtttg accaactact tcttttgaaa
    43441 ttccgctaac aacgattgtt gttgggtttg gaagttcaaa agtaattcct tctggagctt
    43501 caacttcgtc tggatgagat ttaccaacag ccaaaacaag tttagatcct tgaagctgtg
    43561 cacggtaacc aaccccacgc atttcaagtt ctttcttgaa tccttctgat acaccaacaa
    43621 ccatgttgtt caaaagggca cgagtagttc cgtggatagt tttcatttct tttgaatcgt
    43681 ttggacggtg aagagttatt tcagtacctt ccacacggat ttcaatatct tttgagaact
    43741 cacgagtaag ttctccttta ggtcctttta cagttacaac gttgtcattg ttagcgagtt
    43801 caacaccagc aggcaacacg ataactttat taccaatacg tgacatgttt attttctcct
    43861 gttaaattgt caggccagaa cggccagttt tcacggggtt cagatactta tttagttcaa
    43921 gagctgaact gaacacgctc taagaactgt gcaaaataga taagttggct tgtttgtaaa
    43981 caaacttctc caacttccta attttgcttt gttctttacg agctttgtat cttgatttta
    44041 ccaaacgtaa gcgataacct caccaccaac attcttttgg cgtgcttctt tatcagtaag
    44101 caaaccttca gaagttgaaa ggatggcaat tccaagtccg ttaagaactt ttggaaggtc
    44161 ttcacgtttt ttgtagacac gaagtcctgg tttagaaaca cgtttcaagt tagtgataac
    44221 tttctcacca tttggtccgt atttaagaaa tacacggatg acgccttgtt tgtcatcttc
    44281 aatgatttca acgtttttta caaaaccttc gcgtttaagg atttcagcaa tccctttttt
    44341 gatgtttgat gcaggtactt caagtacttc gtgtttagct tggttagcat tacgaatacg
    44401 agttaggaag tctgcgattg ggtcagtcat aaccattttg tttttctcct cttactagta
    44461 gtttgcaagt tgcacttgct agttaataca tgatacaaag agcgtaacag cacgagcaaa
    44521 aatagacaat ttaatgcaga agcaatgctc caaagcaaat tggtcttttt tgccaaagct
    44581 gtagctcgtg ttcaaattgg caagcccaat tgaacccggg ctaaactctg tgtgaaaaag
    44641 ataaactttc ctagaaactt tagtttcttc gtcaagtttc ctatttttac ttggagtttt
    44701 gacgctcttg atatcttaaa ttaccaagat gcttttgtta caccaggaat ttgaccttta
    44761 tgcgcaagtt cgcgaaaagc gatacgactc agaccaaatt tgcggtaaac tgaatgtggg
    44821 cgccccgtaa ctctacaacg attatgtaaa cgagtcggtg aggcgttgcg aggtaattta
    44881 gataaacctt cgtagtcccc tgccgccttt aatgcagcac gtttttcagc ataacggtca
    44941 acaatttttt ggcgtttagc ctctctagct accattgatt ttttagccat tagatttacc
    45001 tcctatatta ttttgcaaaa ggcattccaa ggcctgtaag caatgcacgt gactcttcgt
    45061 cagtgttagc agttgttacg ataacgatgt caagaccacg agttttgtca acgtcatcga
    45121 agttgatttc tgggaagatt aattgttctt tcacaccaag tgtgtagttc ccgcgtccat
    45181 caaatgattt tgttggaaca ccgtggaagt cacgtacacg tggaagtgaa actgatacca
    45241 atttatccaa gaattcgtac atacgttcac cacgaagggt aacttttgca ccgatcgcaa
    45301 caccttcacg aagacggaag ccggcgattg attttttagc tttagtgata agtggttttt
    45361 gacctgagat aagtgccaat tcttcagcag ctttttcaag gcttttagcg tttgatacag
    45421 cttcaccaac acccatgttc aaaacaatct tatctacttt aggcacagcc atcactgatg
    45481 agtagttgaa ttgttctgtc aaagcaggaa ctacttcatt aagatatttt tcttttaaac
    45541 gatttgccat tatacttctc ctttccttcg tgattaatca agcacttcgc ctgatttttt
    45601 gttgtagcga acttttttac cgtctacaaa tttgtaacca acacgaccag ctacaccatt
    45661 tttgtccaaa acttgaacgt ttgatacgtg gatagctgct tctttctcga tgataccacc
    45721 ttgaggaagc tcgttagttg gacgttggtg tttcttaaca atgttaacac cttcaacgat
    45781 aactttgttt acttttggaa gggcagtaag gacaacagct tctgttccct tatctttacc
    45841 agcgattacg cgaactttgt cgcctttttt tacaaacatt aggtttctcc ttgatttttc
    45901 ttacgcccat aagggcaccc tagcttgaag ctaggggact agtttgttcc taaaaattaa
    45961 agtacttctg gagcaagtga cacgatcttc atgaagccac cttcacgcaa ttcacgtgca
    46021 actgggccaa agatacgtgt tccgcgagga gttttgtctt cacggatgat aactgctgcg
    46081 ttttcgtcaa atttgatgta tgaaccatca gcacgacgag cacctgattt agtacgaacg
    46141 ataactgctt taacaacgtc acctttttta accgcaccac caggagtagc ttgttttaca
    46201 gatgccacga taacatcacc gatgtttgca aatttacgtc ctgaaccacc aagaactttg
    46261 atagtcaaga tttcgcgagc accgctgttg tctgcgactt tcaaacgagt ttctgtttga
    46321 atcatttcag ttttctcctt tcaggtttga ttagatgatg accgcttctt caacaacttc
    46381 tacaagacgg aaacgttttg tagctgaaag cgggcgagtt tccatgatac gtacgatatc
    46441 gccttctttg gcaacattgt tttcatcatg agctttgtat tttttagagt agttaatacg
    46501 tttaccatag actgggtggt tacgttttgt ttcaactaca actgtgattg tcttgtccat
    46561 tttgtcagat acaacacgtc caacaagaac tttacgatta ttgcgttcca ttgaaatttc
    46621 tccttcccta gtctattatt tcgcttcaga ttgaactgtt ttgatacgag cgatttgttt
    46681 tttaacttct ttcaagcgag ctgtttgttc caattgacca gtagcagctt ggaaacgaag
    46741 ttcaaacaat tcttttttca attcgttttc gcgcttcgcg agttcttctt gagaaagacc
    46801 acgaagttct ttaacaaatt cttttacttc attaagtttc atgccttctc cttattctgc
    46861 ttcacgtttt acgaatttac atttaactgg caatttgtgg ctagcaagtc gaagcgcttc
    46921 acgtgcaatc tcttcagata caccagcgat ttcgaacatc actttaccac gtttaactgg
    46981 tgctacccaa ccttcaggtg cccctttacc agatcccata cgcacaccga tagctttagc
    47041 agtgtatgat ttgtgtggga agattttaat ccaaacttta ccaccacgtt tcatgtaacg
    47101 agtcatggcg atacgagcag cttcgatttg gcggttagtg atccagtggc tagttgtagc
    47161 ttgaagaccg tattcaccga atgctacttc ttttccacct tttgcttcac cgcgcatttt
    47221 tccacggaac tcacgacggt gtttaacacg tttaggtact aacattggtt atttacctcc
    47281 tttagtgttt ttacgagctg gaagaacttc accacggtag atccatactt taacaccaag
    47341 tttaccgtat gtagtatctg cttcttccca agcgtaatcg atatctgcac gaagtgtgtg
    47401 aagcggaaca gttccttcag agtatccttc agcacgggcg atatctgcac cgttcaaacg
    47461 acctgatact tgagttttga ttcctttagc tccagcacgc attgcacgtt ggattgcttg
    47521 tttttgtgca cgacggaaag caacacgttg ctccaattga cgagcaattc cttcacctac
    47581 aaggtgagca tccaaatcag gttgtttgat ttcgatgatg ttgatgtgta cttgttttcc
    47641 agtcaatttg ttaagttttg cacggagtgc atcaacgtta gcaccacctt taccgataac
    47701 catacctggt ttagcagtgt gaagtgaaac gttaactttg tttactgcgc gttcgatttc
    47761 aatagttgaa actgctgcgt cagcaagttc tttttgaacg aatttacgga ttgcaagatc
    47821 ttcatgaagg taatccgcgt attctttttc agcataccat ttggcatccc aatcacggat
    47881 gatgccgaca cgcataccaa ttggatgtac tttttgaccc acgattttac ctccttattt
    47941 ttctgcaaca gctacagtga tgtgagctgt acgtttgttg attggtgaag ctgaaccttt
    48001 cgcacgtgga cggaaacgtt tcatagttgg tccttcgttt gcgaatgctt cagatactac
    48061 caaattagct ttatccaaac caaagttgtt ttcagcgtta gctacagctg agttcaaaac
    48121 tttcaagatg atttcagcag ctttgtttgg agtgaatgtc aagattgcga ttgcatcggc
    48181 tacgctttta ccacggatgt tatcaagaac aagacgtgat ttacgaggtg aaacacgtac
    48241 tgtacgagcc attgctttag ctgaagtaat ttctgccatt tatgttctcc ttattttcta
    48301 cgtgttttct tgtcgtctgc agcgtgacct ttgtaagtac gagttggtgc aaattcacca
    48361 agtttgtggc ctaccatgtc ttcttggatg taaacaggta cgtgtttacg tccgtcataa
    48421 actgcaatag tgtaaccaat gaaacttggg aagatcgttg aacgacgtga ccaagtttta
    48481 ataacttttt tcttttcgtc gttagcttga gcttcaactt ttttcatcaa atgctcatcg
    48541 acgaaaggtc cttttttaag actgcgtccc atttttatat tttctccttt aaatgttgta
    48601 ccacagcggc ttgcactcct atggagcgct accgagctgg cggatttact agttgcttaa
    48661 gcgactagtt taatattatt tctcgttgcg acgacgaacg ataagtttgt cagatttcgc
    48721 tttcttgtta cgagttttaa gaccaagagc aggtttgccc catggagtag atggtgcttt
    48781 acgaccaact ggtgctttac cttcaccacc accgtgtggg tgatcgttag ggttcattac
    48841 agaaccacga actgttgggc ggataccttt ccaacggcta cgtcctgctt taccaaggtt
    48901 tacaagtcca tgttgttcgt ttccgacaac accaactgta gcacggcaag ttccaagaat
    48961 catacgaact tcacctgatt gaagacgaac aagaacatat ttaccttcag aacccaatac
    49021 ttgagcagat gcaccagcag cacgtaccaa ttcaccacca cgacctggtt tcaactcgat
    49081 gttgtgaatc aaagtaccaa ctgggatgtt agcaagtgga agagcgtttc cgactttgat
    49141 atctgcttct ggacctgaaa cgatacgttg acctacttca agaccttttg gagcgatgat
    49201 gtatgctttc acaccgtcag tgtagtgtac aagagcgatg tttgcagaac ggtttggatc
    49261 gtactcgatt gttttaacaa ctgcttcaac gttgtcttta ttacgtttga agtcaaccaa
    49321 acggtaaaaa cgtttgtgtc caccaccttg gtgacgaact gtgatacgac cgttgttgtt
    49381 acgaccagcc ttgctcttca atgcaacaag caatgatttt tcaggagtgc ttgttgtgat
    49441 ttcagcgaaa tccaaagaag tcatattacg gcgaccgttt gttgttggtt tataaacacg
    49501 aattcccacg atatttcctc cttagattat tcagcttcag cagcaaacaa ctcgattgct
    49561 ttagaatcag ctgtaagtgt gatgatagct tttttagttt tgttagtaaa accagtgtaa
    49621 cgtccaacac gtttagcttt tggttttacg ttgattgtgt taacattggc aactttaaca
    49681 ccttcgaaag cagcttcaac agcttgcttg atcaaaagtt tgtgtgcacg agtgtcaact
    49741 tcaaatacat attttcctgc ttcaagttga gccattgagc tttcagtgat gacaggtttt
    49801 ttgataacat catacaaatt cattatgcaa gaacctcctc gattttagag atagctgctt
    49861 gtgtgacaag aagtttgtcg ctatttgcga tgtcaagaac acttgcagtt gtagcagttg
    49921 caactttcac gtttggaagg ttacgagctg aaagagctgc gaattcattt ccttcttcaa
    49981 ggataacaag aactttagaa tcgatgctca atgctgcaag aacttttgca aattcagcag
    50041 tttttggagc tgtaaatgaa agagcgtcta cagctacgaa tttgttttca gcaacttttt
    50101 cagagtaaac tgatttaaga gctaggcgac gaactttttg tggaagtttg tagccgtatg
    50161 aacgtggagt tggtccgaag acaacaccac caccacgcca ttgtggtgag cggatagaac
    50221 cttgacgagc acgtccagtt cctttttgac gccatggttt gcgtccacca cctgatactg
    50281 cagagcggtt tttaacagcg tgtgttcctt gacgaaggct tgcgcgttgg ctgatgatta
    50341 catcaaacac aactgattca tttggttcga taccaaatac tgcatcgcta agaacaactt
    50401 ggccagcttc tttaccagtt tggtcaaata atgttacgtt tgccattgtg actgatttcc
    50461 cctttcttta ttatttacca gctttaactg ctgatttgat agtgataaga gatttcttag
    50521 cacctggtac gttacctttg ataaggataa cgttcttttc tggaacaact tgtacaactt
    50581 caaggttttg aattgttacg cggtcgccac ccatacgccc tgcaaggttt ttacctttga
    50641 atacgcggtt aggtgcaaca ggtcccatag aacctggacg acggtggtaa cgagaaccgt
    50701 gagccattgg tccacgtgat tgtccgtggc gtttgataac accttggaaa cctttacctt
    50761 tagaagtacc agttacgtca acaacgtctc cagctgcgaa tgtttcaact gtaatttcag
    50821 caccaacttc caagccttca acgtttttga attcacgaat gaagcgctta ggagccgtgt
    50881 tagctttcgc tacatgtcct ttagcaggtt tgttgctcaa tacttcgcgt ttgtcatcga
    50941 aaccaacttg gatagcgttg tatccgtctg tttcaacagt tttaacttga agaacaacgt
    51001 ttggagttgc ttcaataact gttacaggga tcaattcgcc agcttcagtg aagatttgag
    51061 tcattcccac ttttttccct aagattcctt ttgtcatgag aaaatagttc cttttctata
    51121 tttttattca aaaagttttt aacgagcgtt ttttatgctc aagtctaaga tacaaagggc
    51181 gtcaaactca acgtgaaaat aggaaactga cacagtgtct agcagacgcg aggaagttta
    51241 tctttttcac accgagttta gcccgtgttc aattagattg aaacaccagc ctctgctctt
    51301 ttatatttta gattaaagtt tgatttctac gtttacacca cttggaagat ccaatttcat
    51361 caaggcatca actgtttttt gagttgggtt aacgatatcg atcaaacgtt tgtgtgtacg
    51421 catttcaaat tgttcgcgag agtctttgta tttgtgagtc gcacgaatga ttgtgtagag
    51481 gctacgttca gttggaagtg ggattggacc cgcaacttgt gcacctgtac gagtagctga
    51541 ttctacgatt tttgcagccg ctgtgtcaag cgtacggtgt tcgtaagctt tcaaacggat
    51601 acggattttt ttgtttgcca tctttttctc cttttcgtct atttaagata ataggctagc
    51661 tccacaagaa aaccgacgcg gtgttgcgtg gcaatgcaac cgagcgtgtc gcaacctctt
    51721 gcatcaaaac taaggctgta atttacagca ccataataga ataacacaaa tcccctgcga
    51781 ttgcaaggga tttgttaggt tttttcgttt tttggggatg aaactgtttt cctacttttt
    51841 ctttctaaaa ttagggattt tcttctatat ttcttctata cataagttaa gtcctcttga
    51901 catcaaattt atcttctgat tggtccacta agatatccgc cttagactca gtttctttat
    51961 agtatcgcag atactgctcc cgtctcatct gatggctagc caatataaag gatgcatcgc
    52021 gatttctcac agtcgtatct ctagcaaggc gtcgctttaa ttcggtctcc tcatccgtgt
    52081 agaaacagat ggttttttca aagagttcct tgggtagaaa gccaacagac atcccttcga
    52141 caatcaaaat tggtttggct ccagacaaga cctcactagc cttccaaggt tcttcaattg
    52201 tcaagacatc catacccgcc tgcaaggcaa ggatatctct ctgcaaactc tccagttcat
    52261 gcgccactgg cagactggct gtcacctttt gattcggcgc gtccttgggt actaccaaat
    52321 agcgtccgga ggtgatatag ggatctgttt ctagcaaatt tactgtagta gaatctaaag
    52381 cttggtacaa ttcctgtgca aaggttgatt tacctgaagc tccatgaccg tatattccca
    52441 gtgtcctgac tttccccgtc tcgatctctg agactagttg gtctactaag tcttttttct
    52501 tcattctctc ttcacctgtt catagatttc ttttccgtca ttgagcttgt cccaaatcac
    52561 tacttgccca cttttgagcg atttttgcac atcgataatt cgttggttag acgaacctcg
    52621 aaactggagc ataagatttc tcttagttcg atcatatctt ccatcgacaa gaatgtcaat
    52681 cagtgacaag agttccagtt tatctggagt ttccaacatc atttcttccc aagtgtagcc
    52741 cgtccaggac cagatgtcct tgtctggcaa ttccttccga atccgcttaa caagtggcaa
    52801 gagaatccca gtattaagaa aaggctcccc tcctagtaag gttaacccct gaacataagg
    52861 ctgggcaaga tctgccataa tctgttcttc caactcagct gtatagggaa tgcctgcatt
    52921 gaaggaccag gtcgctacat tataacaccc ctcgcagtga aacatacagc ctgctacata
    52981 gagagagtta cgcacgcctt cgccatcaac aaagttaaag gccttgtagt caatgatacg
    53041 accttgacta agttcctcgc ttttccattc ttgtggtttt ggattattca ttcgctacct
    53101 ctatccaata acgctcgact ccattgcgag catcctcaaa tattccacca tttgctagaa
    53161 tgactgctct gctagcagga ttattcacgc tacaggtcac cagagctttc ttgatgttct
    53221 tttccttagc aacttgcaag ccctgacgga gagtctcttt tgcataaccc ttgcctcttt
    53281 cagatggacg gatggagtag ccaatgtggc cagcataatt cagtaacccc tcattcagtc
    53341 tcaatcgcag attcaaaaat cctagagcac aacctgattc accaaacaat acgaactgta
    53401 tcgatggaac gcgattttca ggtagccata ttcccatttc tttattacgg ttaccttcta
    53461 gccactcctc gtaagaaaaa tcctcaacat cccaaaatcc tccatcatgg gctgattggc
    53521 tcttttcaaa ctctgtcatc atatctaaaa cagctttctt atccgctaat cttggtctgc
    53581 gtaattccat ccttacctcc cactatgaga aaagcgagag ctggctctca cttttatttt
    53641 ttcttgttct tgtttaaaaa ttgttctctg aagctaagca agcgttcttt cttaccagct
    53701 ccacctttag agtgtttctc gtgataacgg gtcacttgtg cgcgcccctt atcgtctagt
    53761 tgatattttc ccatttcatt tctttctaat ttgttacttg atgcccagct attttaatcg
    53821 ttgaaccatt catatgtttg acacgcgcag cgatttcctt gtgacgcccg ttgaccatcg
    53881 gtcttgcttg aggattacct aggtagccac aagttcgctt caccacatct actgttttag
    53941 ggtcgctatt gccacagttt ggacaagcaa accctctctc agttggttca aaatcccctt
    54001 caaagtcaca cttgtagcaa cggtcaatcg gagtattggt gcctagatag cctacacggt
    54061 cataagcata atcccagaca gcttccaagg cctttggatt ttgctgaagg actggatact
    54121 cacaataatg gatgaaacca cctgacgcac ctgcttctgg atagactttc tcaaagtcca
    54181 atttttcaaa cggtgttgga tttttacgaa catcgtagtg gaaagagttg gtgtagtatt
    54241 ccttgtctgt gatatcagga atagagccaa acttgtctat atctagtcgg cagaaacggt
    54301 ctgtcagact ttcggatggt gttgagtaga tagagaaatg gtagccatat tggtctgacc
    54361 actcttctac acggcgtttc atatcgtgaa tgatgtctag cgtgaattcc ttagcatctg
    54421 gattactttc ccagctgtta ccaaagaaaa ctgtcgctac ttcatacaag ccgatatagc
    54481 ccagcgaaac ggtcgcacga caattcttaa agagctggtc aacactttct tctttaccta
    54541 gacgatggcc aaaagcaccg tactgataaa ggataggagc attcgctggt gtcgcctctt
    54601 tagtgcgttc gacacggtaa acaagagcat cttctgcgat attcattcgc tcgttgaaga
    54661 tttcccagaa cttattcata tcaccttcag actcaagagc aatacgaggc agattaaccg
    54721 tcacaacacc cagattcatg cgacctgaat tgacttctac accattttca tccttccatc
    54781 cttggaggaa agaacggcaa cccataggca ccttgaagga tcctgtcaag tcaacaatct
    54841 tatcgtaaga taaaacatct ggatacatcc gtttggttgc acactcaaga gccaactgct
    54901 tgatgtcgta gttgggagtt ccttcctcta aattaaggcc tcttttcaga gtgaaaataa
    54961 gtttaggaaa gatagccgta cggtgttctg aaccaagacc cttgatgcga atgtttaaaa
    55021 tagctttttg aatttctcgt tcaaaacgac tggttcccag accaaaacct agcgaagtaa
    55081 aaggtgtttg tccatttgaa gtgaagagag tattgatttc atactcaaga gattgcatgg
    55141 catcgtagat gtccttttgc gttttcttcc aagcgtaatc ttcccgtttt tcaggcaata
    55201 cccactcttc tgcatctttg agatgttttt gatagttctt ctctgcataa ggcgccaaaa
    55261 tttcatcgat acggtcagct gaacagccac cgtactggct agaagcaacg ttggcaatga
    55321 tttgagaaat ctgtgctgtc gcagtctgga tagacttggg actctctacc tctgcatttc
    55381 caatcttaaa accattttcc aacataccct taaaatcaat caaacagcag ttggtcatag
    55441 gggtataggg actgtagtcc aaatcgtgat agtggatatc ccccttttgg tgggcattgg
    55501 ctacgtgctt aggaagcatt tgcagtccga ttgatttccc aacaatccct gctgtcaaat
    55561 cacgctgagt gttaaagaca tcactgtctt tattagcatt ttcattgaca actgtctggt
    55621 ctttgttgag aagtttatga atactaaagt tgatatccgt cgcttttgag cgctcaaaat
    55681 ccctctgtgt ccgataagta atatactcct cagccagcgc atattctttg gcttcaagga
    55741 gttcatgttc tacgatattt tgaatttcgt aaatcttaat tccctgtgga aagcgactat
    55801 gaatttctgt aataattctc tcagtcagat cattgaggcg tttttcaacc aggggtgtca
    55861 catccataac cttgtcagct gccttgtgga gagccttgtc aatcttgtcc acatcaaata
    55921 caacacgtct cccatctcgt ttctcgacga agagagttgg caaaattgta attttttctt
    55981 ctagtgcaat catatgcatg ctcttttcta aaatgttctt tctaaaaagt attataccac
    56041 tctaaaaaga aaaatcaata tcttgtgtta aaaatcctaa aatttttaaa aatacacaag
    56101 atattgattt ggttatttaa ttttatagac tttaaactct gagtaatcag tctttacttg
    56161 ttggtaattt tcttttaaaa gtgtttccac ttcagaccag actaccacct tgtcatttac
    56221 cacaatcacc ttaggttgtt tttctttcaa gtcattaagt aacttattac gattttcctc
    56281 agttgctgta tagtgcaacg gggacaaaat cgccgatggc gacaagcgct cactcttacg
    56341 ataaagagtc gcagtatcat cccatgcata gatagcatct tccgtatttg tctcctcttt
    56401 taccaaatca gcaagacgat tacgttcttg ataaggtact ggataaagga caaacttcat
    56461 caaaaaagga acagacaaaa gatagacaaa cccaaaaact agcagatata gatttccttt
    56521 gaagaaacga ctccataggc taactggcgt ttctcttcgg cttcttctag cactacgccc
    56581 cctctgttga ctcttgatat tggttaccaa taaaagtagg aaaacaggga agacgaccat
    56641 caaataagaa gcgtgcagag gctcttgtga aaagaaaagt aaaatcaaac ctaaaattac
    56701 cacaagactt gcaggaaccg aaatgacgta tagtttaaag ggtttggatt gaaagagacc
    56761 taaaaagata caaaccacaa atcccaaacc aagggacaac aagccataaa aaaacatatt
    56821 ctctaaaatt ttagaagtaa aatcaaagcg aatactgtca ataggataac gaataccact
    56881 aatcgcatcc ccaaaacttc ctgttgcagc actatagtag gcagttggat aaaagacaag
    56941 tgaaaaacct aaagccactg caagaaactg ataaaaccca tgcgcaaagc gtctatgccc
    57001 aaggttaaag accaacaagc ctaaactcac tacagcaata aacaggagtg atgataaggg
    57061 agcaaagaaa aatccgcctg ccaaagctaa cccaatccgt acaaatccct tatcatggct
    57121 tggattgctt aggtaagccg caactaaact aaaggctgcg aataagaaag gaagcgctaa
    57181 aagagtcgca tagcctccac caaaagcaag acctgtaact agcatgtaaa aaatagtcac
    57241 cagatgtcca gcttggtctc cttgctctgt caaggtgtcc gctgatctaa aaaggaaaaa
    57301 tcctcctgct accaaggcta accactcaaa gatggcaaag aaaaatccgc cctgactcac
    57361 gtaagtcagc aaataataaa gcaaaccttg acttccataa tagtcgctgt aaatcttccc
    57421 tgtctgatga agcgcccaac ctgcataaaa atcctgcact tcttgtgcac tcattaagtc
    57481 gagtaatagc ggtactccta gagttatccc cgttacaagc gtactccata gtaaaatttt
    57541 caccaaagga agacgacttg attcacgatg atgcgattct tgctcgattt ggtattctag
    57601 aggttcacga ttctccttat gaacttcttc tactctacca tacacactca tatcgtttct
    57661 cctgttcaat ttatctgtct tagtatatca aaaagtccta ggattgtcag cttgcgatgg
    57721 ctgtttgagt gattttttgc atagtttcac aaaggtttca atttcccgaa agcggtgtaa
    57781 atgtgtctgt ttgaactcaa tcatatcatt cacttcttcc ttagttagca tgaattcccc
    57841 tcctctttct ttctattcgt aaaagacaag aaaaataggt cgggtttgta tcctaagttc
    57901 tagatttctt ataagatagg ggcgaatagc tgggcgattt cttttacaag cttttgatac
    57961 cagctcgttt tgatagaatg aggatagact tcctgagaat ctgcaaatat atttcgaaaa
    58021 tctcgggcga tttcccttat agaaggagtt ttatagagta agactgcatt ttcataatgg
    58081 tgtaccaagc tccggtagtc gagattgatt gtccccacca ccgcaaaatc ttcgtctacc
    58141 aacatctgct tactatgaat aaaacctgga ctatactcat aaatccgaac accagcagaa
    58201 agaaggtcgg gataagctcc tctcgtgact aactgaatga acttcttatc tggtatgtaa
    58261 ggggtgataa ttcgaacatc gacccctctc atagctgcat ttttgattgt ctctgttaaa
    58321 tcataatcta taatcaaata aggtgtcgta atatagaccg attccgttgc ttgattgatt
    58381 aaactctgat aaactttttt ccctacctgc gctcgaaaaa ttggcttggg tccacttccg
    58441 tatggaatgg ttaaaccgtc actcgggata gaatgatttt ctaaatgata ttgatcaaaa
    58501 tcactaattt ctcctcgatt aatgtaccaa gtggtcaaaa ataagcgtgt cagggctttt
    58561 actgctagtc cgtctaagcg aattccacta tccttccaat aaccaaatct ctcgacgtgg
    58621 ttaatgtact catctgccag attgacccca ccagtatagg ctatctgacc atcaacaatc
    58681 aatatttttc tatgatctct gttattataa gccactgtca aacgaggaat aactttattg
    58741 aatttatggg cctcgatgcc cagctgacga agtcgatgtg cataatctcc tgttaaagta
    58801 gccatacagc cgatatcatc atagagcatc ttaacctcta caccctgagc taccttttgc
    58861 tctagtatat ctagtatgcg attccacatc aaaccttctt ctataatgta atattccaag
    58921 aaaataaatt tctcagcctt tttaagatct tctaccatct ttttccacat agcttctccg
    58981 ttaggaaaaa atgtagaggc agtttgatca tagatgtcag cattggtatc catactcaat
    59041 aaggacttga tgactccata agctgccttg tcactttctt ttaatttctc ttttagtagc
    59101 tggctatttg cttcttggaa atgcatagag cctagcttct tcagttgttt gatttctttt
    59161 ttggacaatc gcctttcacc aaacatcaga tagagtaagg gaccaaatac tggcacaaag
    59221 gctactaaca accaggttac cttattctca ggagtcgtat tacggttgac tatcgaaatg
    59281 attgtgatga tactcagtag aatgaggaca gtaatccata aaatgggagc catgcgtcct
    59341 aaataaagaa agagaccaaa aacaagacaa agttcaagta gcataatcga aagactaaag
    59401 ccatacttgg acatcaataa ttgaaatttc ctatatttca tatcccctcc ttgatagttt
    59461 gaatgctaaa aacaagtaat tgatactagt ataacgcagg tattcggtag aaggcaagta
    59521 tttatgatta ttttctaggt aaaagtaaaa aggctgaaaa atccagccct ttctacttgc
    59581 atagtcttat ttagttaaaa cgatttggtc cgatgggtct ctatccatat catctacgac
    59641 gatttgatta ccgtttacag tgtaaatttt gacatcatcg ccaataatca tgcgttgatt
    59701 tgctgaatca aatgtaacct gtttgacttt ctgatccccg tcagattcga gctcagtcca
    59761 tgtaccagtc gttccagtta ccactaaagt gatacggtct ccgtcatcct gaccagtata
    59821 agtcccatca atatcggtcg gttgagcaac aggttggttt tgtactggag ccgcttgatt
    59881 ttggttacct cctgcatttg cagtattggt attctgttga gcaggttgtt gagcaggctg
    59941 ttggacctgc tgctgagcaa caggctgttg aacctgttga gctctttgtg aacaagctac
    60001 taagagacta aaacttgcta gagaagcaag agaaagtgtg aatcttttta atttcatgat
    60061 gaatttcctt tctgctacca atttagagaa attttctcta accagcaatt cccctagtat
    60121 aacaagttca aacaaggggg tcaatttatc tgctcacggt ccagcaggta gccccgtact
    60181 tctgagataa aatagagaga ccctgtaacg aacagcaagt cttgagcgtc tgccctttct
    60241 tcgaaactgc tgataaattc tcggcaagaa gaaactatat cgtaacctgt cacatccctt
    60301 tcgtccaaag ccccctgata gtcaaagccg gtcaccttga gttccacctg aggcaatttt
    60361 tcagtcaggt aacctaacat cccttgataa tccttccgtt tcaaggctcc aaaaaggatt
    60421 tgaggtcgat agccttcctg ctctttttct ttgataaact caaccaagcg agtcaaggca
    60481 gggaggttat gagcaccatc caagtaaatc tgtgggcgaa tacgctccaa gcgaccagcc
    60541 caatgggtct gttccaaggc ctttcttaca gcctgttcat caacagcttc ctttccttct
    60601 cccataaaaa gaagaaaagt ttgcaacgcc aaggctgcat tctcctgctg atagactcct
    60661 tctaagccta tgttcagctg tgaaaaattt agtaaagagc ttgaaaaatc accattcagc
    60721 attaaaaaat cttgacctgc ctgataaagg ttaacagcta aagattcggc ttttttctga
    60781 cagacaagcc tagcttctgg aggcaatttc gcaatcactg ccttcttacc agccttgaaa
    60841 ataccagctt tctgctctgc aattgcttct agactatcac ccaaggtttc ttgatggtca
    60901 agcccaatgg aggtgatgac agcaagctct ccagttacca cattggtcgt gtcaagtaag
    60961 ccaccaattc ccacttctaa taaaaccaaa tccacctctt gctccctaaa ataaagaaaa
    61021 gcaaccaagg tcagcatttc aaaaaaggac aactggtcag gagtttgcag aagcgttttc
    61081 tccatctcct tgacctgatc agccaaacgg ataaagtctg cgtctgctat aggttgccca
    61141 ttaatgcaga ttcggtcatt gatagagacg atatgagggg aggtaaaggt cgcaactttt
    61201 ttgccatgcc ccataaataa ttccctcata aaagcaatgg tagatccttt cccattagtc
    61261 cctgttacgt ggataatagg gtaagactgc tcaggatttc ctaacaaatc caccgcttgc
    61321 tgtattcggc ccaaacctga tcgaaaattc aaaccaatcc gactatgaag ccattcttct
    61381 acttcaaaca tacacatctc cttgacaaaa gtccaatcaa ttatcgcatt aaagtatggt
    61441 tactaataaa aacaaggcca ggattttcgt cccgacctct tacctggtta gctaataact
    61501 agccactatg aatgtgaata tgggctaaaa acatccacta gatgttccaa ctcttcccca
    61561 tttctgggag ttgggctgat acagtctccc agactgtatc actcctccat aaagctgttg
    61621 aagacttctt caatcatgtt ccattcgtct tctgagtctt ctgggattgg ttgcaattcg
    61681 ccttctgttc catcttcatt ttcaatgaat gagtaagctt ggatttcaac ttgtccgtct
    61741 tcgtcttctt ctgcgttaac tggtactaga agaacatagt ttttaccaaa ttcttctttt
    61801 ccatcaattg tcaaaaggat ttcaaacaag gtttcatttc cttgctcatc tactagtgtg
    61861 attagttcac gttcttcgtg gtcgtggtta tgatcgtgtg acatagcctc tcctttatat
    61921 taaaattttc tatctaaata attttgtaaa atcagctgag ctgctaactt atcaatgact
    61981 ttcttgcgct tattgcgact gatatctgct tgttcaatca acatgcgctc agcagccact
    62041 gttgtcaagc gttcatcctg atagtctaat ggtaaaccaa aaaactcttc tagctttgct
    62101 ccgtaggctt gactagcttc tacgcgcggt ccacttgtat tgttcatgtt tttaggcaag
    62161 cccactacaa atcgttccac cttgtaagta tcaaccaatt ccttaacgcg gtcaaaacca
    62221 aattggcctt gttcttcatt tatctggatg atttcaagcc cttgagctgt aaaaccaagc
    62281 ggatcgctaa tcgccacccc taccgttttt gaaccgacgt ccaatcccat aattctcata
    62341 ggttatagat cgactccttg tcctttgagg tagtagcgaa ccaattcctc aacgatttca
    62401 tcacgctcat acttacggat ttgatttcgt gcattattat aacgaggaac gtaggcaggg
    62461 tctccactca atacgtaacc tacgatttgg ttaattgggt tgtaaccctt atcgttcaac
    62521 gaagcataaa catctgtcaa agtttcgcta atttcttttt tattggaatc gtccaattta
    62581 aaacgtactg tttcttcagt aaatcccatt ctaacaccct ctttccttag aatagtacca
    62641 ttatagcata attccttacc ttctacaatt caggcagtct atttatttgg attttctatt
    62701 gttctgtcgc gccatttgcc aatctatctg aaatatattt gcttggttca tttttcaaaa
    62761 gattttccaa accaatattc ttcagatgtt ccaactggga agccttcttg acatccagaa
    62821 cttgaaaatc aaaactagtc gttgtttgaa gttccgttgc gctcaatagt tttgtttcaa
    62881 gtttgaaacc tgccaattta cgagcttcaa tgatagactt atccttctcc tccgcttcaa
    62941 gaagagcttt ttgagtttcc tccactccat gttggtcaat ataagtcttc aactcatcag
    63001 agactggacg tttttgttgg atagttagac tcaaaaaagt cttgtccttg tatgtaatgg
    63061 tttgtgtctg ctggctacca tcatctgact tgggcaagac caaagtctta gttacaactg
    63121 tattcttctc agcattttca ataactggca atgccgactg aagcgtatct ttttctgttt
    63181 ttgtagctgg tccagtttct tttttctgtc cgcaaccaac caggacaaaa aggaaagcta
    63241 gactaacaag aactattttt ttcatttctt tcttctttct ttttgaaatt aaaatagaat
    63301 aagactggga agtgctccca gccttgatgt ttatagagct gcacgcaaac gtgcttctgc
    63361 attttctaca ttacggacag agcgtggtag gaaggcacga atatcgtctt ccttgtagcc
    63421 aacttgcagg cgtttttcat ctacaaggat tgggctcttt aaaattctcg gtgtttccat
    63481 aatcagattg agaacttcat tgacactcaa atcttcaata tccactccaa gggctttggc
    63541 atagcgattt ttagacgaaa cgatgctggc tattccgtta tctgttttgg ttagaatatc
    63601 cagtaattct tctctcgtaa ttccttcttt accaaggttt tgttctttat aacttaactg
    63661 gtgggcattg agccaggttt ttgctttttt acagctagta caacttgaga ctgtataaat
    63721 tttaatcatg tacctacccc tttcgctaca tgttactatc agtttagtct attataccat
    63781 aaaaaacatc cgacttgcga cctattttta at
//
LOCUS       DAVBCG010000002        48492 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBCG010000002 DAVBCG010000000
VERSION     DAVBCG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.002"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(72..1208)
                     /gene="lctO"
                     /locus_tag="VQ495_000076"
     CDS             complement(72..1208)
                     /gene="lctO"
                     /locus_tag="VQ495_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2530101.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1833..3323)
                     /gene="lysS"
                     /locus_tag="VQ495_000077"
     CDS             complement(1833..3323)
                     /gene="lysS"
                     /locus_tag="VQ495_000077"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2530102.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3451..3597)
                     /locus_tag="VQ495_000078"
     CDS             complement(3451..3597)
                     /locus_tag="VQ495_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2530103.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            3823..4491
                     /locus_tag="VQ495_000079"
     CDS             3823..4491
                     /locus_tag="VQ495_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2530104.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            4451..5128
                     /locus_tag="VQ495_000080"
     CDS             4451..5128
                     /locus_tag="VQ495_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2530105.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            5129..5886
                     /locus_tag="VQ495_000081"
                     /pseudo
     CDS             5129..5886
                     /locus_tag="VQ495_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            5898..6759
                     /locus_tag="VQ495_000082"
                     /pseudo
     CDS             5898..6759
                     /locus_tag="VQ495_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(6839..7486)
                     /locus_tag="VQ495_000083"
     CDS             complement(6839..7486)
                     /locus_tag="VQ495_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530106.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(7483..8181)
                     /locus_tag="VQ495_000084"
     CDS             complement(7483..8181)
                     /locus_tag="VQ495_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530107.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(8191..8943)
                     /locus_tag="VQ495_000085"
     CDS             complement(8191..8943)
                     /locus_tag="VQ495_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530108.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(8943..9707)
                     /locus_tag="VQ495_000086"
     CDS             complement(8943..9707)
                     /locus_tag="VQ495_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530109.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(9803..10206)
                     /locus_tag="VQ495_000087"
                     /pseudo
     CDS             complement(9803..10206)
                     /locus_tag="VQ495_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10386..11018)
                     /locus_tag="VQ495_000088"
     CDS             complement(10386..11018)
                     /locus_tag="VQ495_000088"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2530110.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(11052..11753)
                     /gene="pyrF"
                     /locus_tag="VQ495_000089"
     CDS             complement(11052..11753)
                     /gene="pyrF"
                     /locus_tag="VQ495_000089"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2530111.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            12074..12166
                     /locus_tag="VQ495_000090"
                     /pseudo
     CDS             12074..12166
                     /locus_tag="VQ495_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(12488..13201)
                     /locus_tag="VQ495_000091"
     CDS             complement(12488..13201)
                     /locus_tag="VQ495_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2530112.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(13194..13889)
                     /locus_tag="VQ495_000092"
     CDS             complement(13194..13889)
                     /locus_tag="VQ495_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530113.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(14229..15561)
                     /locus_tag="VQ495_000093"
                     /pseudo
     CDS             complement(14229..15561)
                     /locus_tag="VQ495_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            complement(15545..16343)
                     /locus_tag="VQ495_000094"
                     /pseudo
     CDS             complement(15545..16343)
                     /locus_tag="VQ495_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(17133..18332)
                     /gene="divIB"
                     /locus_tag="VQ495_000095"
     CDS             complement(17133..18332)
                     /gene="divIB"
                     /locus_tag="VQ495_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2530114.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(18342..19400)
                     /locus_tag="VQ495_000096"
     CDS             complement(18342..19400)
                     /locus_tag="VQ495_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2530115.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(19402..20754)
                     /locus_tag="VQ495_000097"
     CDS             complement(19402..20754)
                     /locus_tag="VQ495_000097"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2530116.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(20858..21499)
                     /locus_tag="VQ495_000098"
     CDS             complement(20858..21499)
                     /locus_tag="VQ495_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530117.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(21501..23522)
                     /locus_tag="VQ495_000099"
     CDS             complement(21501..23522)
                     /locus_tag="VQ495_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2530118.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(23593..23892)
                     /locus_tag="VQ495_000100"
     CDS             complement(23593..23892)
                     /locus_tag="VQ495_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2530119.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(24258..24512)
                     /locus_tag="VQ495_000101"
     CDS             complement(24258..24512)
                     /locus_tag="VQ495_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2530120.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(24532..26373)
                     /gene="typA"
                     /locus_tag="VQ495_000102"
     CDS             complement(24532..26373)
                     /gene="typA"
                     /locus_tag="VQ495_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2530121.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            26535..27260
                     /locus_tag="VQ495_000103"
     CDS             26535..27260
                     /locus_tag="VQ495_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2530122.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(27300..27527)
                     /locus_tag="VQ495_000104"
     CDS             complement(27300..27527)
                     /locus_tag="VQ495_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530123.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(27616..27996)
                     /locus_tag="VQ495_000105"
     CDS             complement(27616..27996)
                     /locus_tag="VQ495_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2530124.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(27996..28265)
                     /locus_tag="VQ495_000106"
     CDS             complement(27996..28265)
                     /locus_tag="VQ495_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2530125.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            28329..29237
                     /locus_tag="VQ495_000107"
     CDS             28329..29237
                     /locus_tag="VQ495_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2530126.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(29291..30046)
                     /locus_tag="VQ495_000108"
     CDS             complement(29291..30046)
                     /locus_tag="VQ495_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2530127.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(30067..30996)
                     /locus_tag="VQ495_000109"
     CDS             complement(30067..30996)
                     /locus_tag="VQ495_000109"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2530128.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(31011..31634)
                     /locus_tag="VQ495_000110"
     CDS             complement(31011..31634)
                     /locus_tag="VQ495_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2530129.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(31627..32865)
                     /gene="hflX"
                     /locus_tag="VQ495_000111"
     CDS             complement(31627..32865)
                     /gene="hflX"
                     /locus_tag="VQ495_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2530130.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(32858..33742)
                     /gene="miaA"
                     /locus_tag="VQ495_000112"
     CDS             complement(32858..33742)
                     /gene="miaA"
                     /locus_tag="VQ495_000112"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2530131.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33863..34033
                     /locus_tag="VQ495_000113"
     CDS             33863..34033
                     /locus_tag="VQ495_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2530132.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(34081..34920)
                     /locus_tag="VQ495_000114"
     CDS             complement(34081..34920)
                     /locus_tag="VQ495_000114"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2530133.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(35017..35976)
                     /locus_tag="VQ495_000115"
     CDS             complement(35017..35976)
                     /locus_tag="VQ495_000115"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2530134.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(36103..37101)
                     /locus_tag="VQ495_000116"
     CDS             complement(36103..37101)
                     /locus_tag="VQ495_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2530135.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGTFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLTSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            complement(37151..37768)
                     /locus_tag="VQ495_000117"
     CDS             complement(37151..37768)
                     /locus_tag="VQ495_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2530136.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(37769..39490)
                     /gene="pabB"
                     /locus_tag="VQ495_000118"
     CDS             complement(37769..39490)
                     /gene="pabB"
                     /locus_tag="VQ495_000118"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2530137.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(39559..45246)
                     /locus_tag="VQ495_000119"
     CDS             complement(39559..45246)
                     /locus_tag="VQ495_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2530138.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            complement(45268..45831)
                     /locus_tag="VQ495_000120"
     CDS             complement(45268..45831)
                     /locus_tag="VQ495_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2530139.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(45947..47638)
                     /locus_tag="VQ495_000121"
     CDS             complement(45947..47638)
                     /locus_tag="VQ495_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2530140.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(47635..48372)
                     /locus_tag="VQ495_000122"
     CDS             complement(47635..48372)
                     /locus_tag="VQ495_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2530141.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        14570 a        10466 c         8484 g        14972 t
ORIGIN      
        1 ttttcgatag caatttattc ttccaataca cgaagaaaaa cctccacatt cagtggaggc
       61 aatctgtttt atcaatacaa ttttaagtca cgagggtcaa ctgggaaggt tgggttgtat
      121 ggattgtgac ggagcttaaa gtgtttgaca tcttcaatgg tctgagctcc agacaattgc
      181 ataactgtct tcaattccgc attcaagtgt tcaaagactt gacgcacacc gacactacca
      241 ccgagagcca agccataaat aacagggcga ccaatagcta ccaagtctgc tcctgaagcc
      301 aaggctttaa agacgtgttg accacgacga acaccagagt caaagacaat cggcacacgt
      361 ctatcaactg cttctgccac ttcttgaagc gagtcaaagg cagctggtcc accgtcgatt
      421 tgacgaccac cgtggttggt tacccagata ccagaagctc ctgcagcaag tgaacgttca
      481 acgtcctcac ggcattgtgg tcccttgaca tacacaggaa gaccagagta ttcagcgata
      541 aattctacat cgcgtggaga caagcgttgt ttagctgatt tgtaaacaaa gtccattgat
      601 ttaccagcac cttctggcag gtattcttca acaatcggca tgccaactgg gaagacaaaa
      661 ccattacgct tatccacttc acgattcccc cctacagtag catctgccgt caagacaatc
      721 gctttataac cttcagcctt cacacggtcc atgatgtggc ggttgatacc gtcatcctta
      781 ctaaagtaaa attggaacca atggggtgtc ccttgaaggg cttcagaaat ctctggaagg
      841 tcaacagtag agtaagagct ggttgtataa agagaaccaa actcatgcac accacgcgca
      901 gtcgccactt ccccctgttc atttgccaat ttatgagccg caacaggcgc cataatgatt
      961 ggtgaagata gtttttcacc tgcaaattca atctctgtac ttggattttc aacatcgcaa
     1021 agtgtatgag gaacgatgag tttgtggtta aaggcgcgga tattctctct taaagtgaaa
     1081 gtatcttccg ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc
     1141 attggctcca aatcataggt attgatgaag tctacatgac cttctgcatt gcttgttttg
     1201 tatgacataa aatgccctcc ttgattaagt aagcgtttgc tttttactac acaactataa
     1261 taactctttt ttcaatcatt ttcaagagtt ttgcttaaaa accatcttat tttttcatac
     1321 aatatactag ccctactttc cttaaactct aaactttagt ctatattaag tgaatcgttg
     1381 agattttttg ctgccaggca tttgaactac ttttttattt gcggattcta tctactaata
     1441 ctataaaaaa acaagtgaat aagacaccag aacatcctct aataggacct atggacttat
     1501 atcagtccca tatatactgt ttaagcaaga tactatccta gttgacgcta acttcctatc
     1561 ccacttatta attttttatt tatagtgaat ctgaacaaat caattgggga atccaagtca
     1621 atttctagta ctttttgaac agtttcagcg tactatcgta aaatgaaata aaacatgcac
     1681 aaattgctta gggaatttaa accaatttct aacaatgtct tagaaatcaa agtgtactat
     1741 ttcaacttca atatactaca cttcattttc tccttaaatt cagtctcttt ttgaatcaaa
     1801 aaatcccctg gtaagagcca gaggataaga atttatttca ttgttgggaa gagcaataca
     1861 tcacggatag ttgttgtatc agtgaggagc atgcagagac ggtcgatacc gattcccaaa
     1921 ccacctgttg gtggcatacc gtattcaaga gcttcaatgt agtcatagtc gatgcctgtc
     1981 gcttcatcat caccaagttc tttggcttta gcttgggctt caaaacggct aagttggtcg
     2041 attgggtcgt tcaactcagt aaaggcatta ccgtactcct tagtcatgat aaagagctcg
     2101 aaacggtcag taaagcgttg gtcttcagga tttttcttag cgagtggaga tacagctact
     2161 ggatgtccat agacaaaggt tggttggatt aaagtttctt caacaaactc ttcaaagaag
     2221 gcattgatga tgtgaccaac ctcagtgtag tgtttctcaa ctggaacttt cttctcagca
     2281 gcgatagttt tagcttcttc caaagtcatg tcttgccaga aatcgacacc agtaatttct
     2341 ctgatagcat ccaccatatg aacacgctta aatggttcgt taatcttgat ttcagtacct
     2401 tggtagttga ctgggccatc acctttgact gatttagcag cgtgttggat aatgccttca
     2461 gtcaagtcca tgatgtcttg gaagtctgca taagcttggt aaacttcgat agaagtgaac
     2521 tcagggttat gagtagcgtc cattccttcg ttacggaaga tacggccaat ttcatagaca
     2581 cgttccatac cacccacgat aaggcgtttc aagtgaagct cagtcgcgat acgaagcacc
     2641 atgtcaatgt tttgggcatt gtggtgggtg ataaatggac gggcagcagc accaccggct
     2701 tcattatgaa gaacaggtgt ttccacttca aggaatcctt tttggtcaag gtaacgacgg
     2761 atttcagaga tgatttttga acgagtgaca aagcgttcaa agctttcacg attagaaatc
     2821 aagtcaaggt aacgtttacg gtaaattgtt tcaacgtctg tcaaaccatg gaatttctca
     2881 ggaagaggac gaagagcctt agacaagtgt gtgatgtggg ttgccttgat agagagttct
     2941 cccatatccg tacgcatcac ttcaccttcg acaccaagga agtcaccaag gtctgctttt
     3001 ttgaagattt cgtagttttc ttcaccgaca gcatccttac gaacgtagat ctgaatctgg
     3061 ccttcgcggt cttgaaggtg ggcaaaacca acttttcctt taccacgttt ggttatcaag
     3121 cgtcctgcga tagtagctgt ttcgttttta tcgtgtaatt gttctttatc gaggttggca
     3181 tatttatctt ttaattcttg tgaatttgca gtacgttcaa aacgttttcc gaaaggatcg
     3241 attccttgtt cgcggagcgc agccattttt tcacggcgaa cgatctgctg gtcatttagt
     3301 tcttccatat gttctgtaga catgggatcc tcctagtttt actcaaaatt gaatacgatg
     3361 agtcattttt tgcgatactc ccattttatc ataaataagc aagaaattca cggataatct
     3421 ttaaaaacta aaaagaacct gacgctaaaa tcactggaaa gaagtgaaag tatctcccgc
     3481 cccactagcg atatagccaa atgctgcttt aggaataact tgttgcgcca ttggctccaa
     3541 atcataggta ttgatgaaat ctacatgacc ttctgcattg cttgttttgt atgacataaa
     3601 atgtcctcct taataagtaa gcgtttactt tgtgtattac aaaaatatct taactctttt
     3661 tcaaaacttt taaaatattt tgtttggaaa tttcagaaat tttatgtcta tgataaaaat
     3721 ccttataacg gcaataaaaa atagatatta tccaaagaag attttaagtg ctacaataac
     3781 tgtattattt ctagatggga ggttctattt ttggattgat ccattgttga acaatatcta
     3841 ccactatatc aaaaggcatt ctttctgacc ttgcatattg cagtttgggg aattttggga
     3901 tcctttctgc tcggtttaat cgttagtatc atccgacatt atcgaatcct tgttttggcg
     3961 caagtagcga cagcctacat tgaattgtca cgtaatacgc cccttttgat tcaactcttc
     4021 tttctctact tcggtcttcc ccgaatcggg attgtcctat cttcagaagt ctgtgcaacg
     4081 cttgggcttg tctttttagg aggctcctat atggcagaat ctttccgaag tgggctggaa
     4141 gccatcagtc aaacccagca ggagattggc ctcgctattg gtctgacacc tctacaggtc
     4201 ttttactatg tggttcttcc gcaagcaaca gcggtggcac tcccctcctt tagtgccaat
     4261 gtcattttcc ttatcaagga aacctctgtt ttctcagcag tggctttggc cgacctcatg
     4321 tacgtcgcca aggatttgat tggtctctac tatgagacag acattgcgct agctatgttg
     4381 gtagttgctt atctgatcat gctgctaccc atctcactgg tctttagctg gatagaaagg
     4441 aggctccgcc atgcaggatt cgggaatcca agtactcttt caaggaaata atctcctgag
     4501 aatcttacag ggattgggcg ttacgattga gatatccatc ctgtctgtcc tcttatccat
     4561 gatgttcgga acagtcatgg gaatcatcat gacctcccat tctagaatca tacgattttt
     4621 aacacgattg tatctggact ttacccgtat catgccccag ctggtgctac tcttcatcgt
     4681 ttactttggc ttagctcgaa actttaatat caatatctca ggtgagactt cagctattat
     4741 cgtttttacc ctctggggag cagctgaaat gggagacttg gtacgtggag ctatcacttc
     4801 tctccctaaa catcagtttg aaagtggaca ggcactcggc ttgactaatg ttcaacttta
     4861 ctaccacatc atcatcccac aagtcttaag aagactgcta ccgcaggcta tcaatcttgt
     4921 cactcggatg attaaaacca cttcattagt tgttttgatt ggggttgtgg aagtgaccaa
     4981 agttggacaa caaatcatcg atagcaatcg cctgaccatc ccaactgctt cattttggat
     5041 ttatggaacc attctagtct tatatttcgc agtttgctac cctatttcca aactatccac
     5101 tcacttagaa aaacattgga gaaactaaat gtctgaaact atcttagaaa tcaaggaact
     5161 aaaaaaatcc ttcggagaca atcccatcct ccaaggactt tctctagaaa tcaaaaaagg
     5221 gaagttgttg tcatcctagg gccatctggt tgtgggaaaa gtaccctcct tcgttgcctc
     5281 aacggcttag aaagtattca aggtggagat attcttctgg atggtcagtc tatcgttgaa
     5341 aataaaaaag attttcacct agttcgccaa aagattggca tggtctttca aagttatgaa
     5401 ctctttcccc atctggatgt cttacaaaac ctcatcctag gccctatcaa agctcaagga
     5461 agggacaaga aagaagtaac ggaagaagct ttgcaattac tagagcgtgt cggtttgctg
     5521 gataaacaac atagctttgc ccgtcaatta tctggtggac agaagcaacg tgttgcaatt
     5581 gtccgtgccc tcctaatgca tccagaaatc atcctttttg acgaggtgac tgcttcgctg
     5641 gatccagaaa tggtgcgtga ggtgctggaa cttatcaatg atttggccca agaaggccgt
     5701 accatgattt tagtaaccca cgaaatgcag tttgcccaag ccattgctga ccggattatc
     5761 ttcctcgacc aagggaaaat cgctgaagaa ggaacagctc aagccttctt taccaatccg
     5821 caaaccaaac gagcccagga atttttaaac gtctttgact ttagccaatt cggctcatat
     5881 ctataaagga gattcttatg aaactattca aaccactctt aactgtttta gcacttgcct
     5941 ttgcccttat ctttatcact gcttgtagct caggtggaaa cgctggttca tcctctggaa
     6001 aaacaactgc caaagctcgc actatcgatg aaatcaaaaa aaaagcggtg aactgcgaat
     6061 cgccgtgttt ggagataaaa aaccgtttgg ctacgttgac aatgatggtt cttaccaagg
     6121 ctacgatatt gaactaggga accaactagc tcaagacctt ggtgtcaagg ttaaatacat
     6181 ttcagtcgat gctgccaacc gtgcggaata cttgatttca aacaaggtag atattactct
     6241 tgctaacttt acagtaactg acgaacgtaa gaaacaagtt gattttgccc ttccatatat
     6301 gaaagtttct ctgggtgtcg tatcacctaa gactggtctc attacagacg tcaaacaact
     6361 tgaaggtaaa accttaattg tcacaaaagg aacgactgct gagacttatt ttgaaaagaa
     6421 tcatccagaa atcaaactcc aaaaatacga ccaatacagt gactcttacc aagctcttct
     6481 tgacggacgt ggagatgcct tttcaactga caatacggaa gttctagctt gggcgcttga
     6541 aaataaagga tttgaagtag gaattacttc cctcggtgat cccgatacca ttgcggcagc
     6601 agttcaaaaa ggcaaccaag aattgctaga cttcatcaat aaagatattg aaaaattagc
     6661 aaggaaaact tcttccacaa ggcctatgaa aaaacacttc acccaaccta cggtgacgct
     6721 gctaaagcag atgacctggt tgttgaaggt ggtcattaac tcttaaaagg gactggattt
     6781 taagctccaa tcccttttta agattttacc tataacatcc tgagtctatc taagatgttc
     6841 aatctgaaca cagtgtacat actttatctt ctattgcata tactttatca cataagatac
     6901 gaatatcctc ttcactatga ctagcaatca aaattgttgt ccctttttca ctagagagct
     6961 ttctaaacaa tgttctcata ttttctacac ttgatttatc caaggcattc ataggttcat
     7021 ctagtaaaag aatagaggga ttctccatga ttgcttgagc aatccctagc tttttcctca
     7081 tacctagcga ataagtttta actttctggt ctttttgctc atatagacca actattttca
     7141 gtgtatcatt gatttcctga ttaccaacta ctcctcgtat gcttgccaaa tattgtaaat
     7201 tcttaaagcc actataataa tttataaaac caggttcttc aatcaaagct cccaaattag
     7261 ctggaatttt tctctcagga acaatatttt ccccattgat taacacttct ccataagacg
     7321 gactatataa accagctatt aatttaaaca atacactttt ccctgagcca ttcgcaccag
     7381 taattcctat aatttccccc tgtttacaac taaagttaag gttttgaaaa acacatgtct
     7441 tttttaattt caactcaata ttttttaata taattatttc attcattcta taaacctcct
     7501 cttttgacga gtgaaataga aaatgctttg aaaaagaaag actaaaaata gcaactgaag
     7561 aaacaaatct ccccctacat ccccattccc tcgattcaaa atataaaata gataattagt
     7621 tcgatttcct acaaatagac caccaaacac aatcatgagt aaaaagaaac taacgcaagc
     7681 aaagttcatt tgtacattcg ctcctagtgc gtataacata aagcaaaaca agactaacac
     7741 acaagcattc aaagaatcaa ctataaacaa aactcctata tcccagtttg gtaactgtaa
     7801 cataaattga atcactagaa cttttaccat gattaaaatg agtccataaa gaaaacaggt
     7861 ccaaatctga tacctaatcc agagcttaat agaagaaaat cgaatcactt ccataaagaa
     7921 atcggatgct cttctacgat atacaactgc tagaaaaaat gtatgaataa taaaccaatg
     7981 aagcaaccat atagataaga ctaaaaaaga gttttgactc agattaggcc cttttaaaat
     8041 ttctataaca agtctatcat ttccttctaa ccaataccat aatcctaaac aaatcaatat
     8101 ccaaaaaaga acataacccc aaaagtactt ggtatagatt atcttattca acctccacat
     8161 gtgactactt aactttccca tttccaactt ttacactctc ctccagtttc tatgaacaga
     8221 aaatacagtc aatggaatta tgatagatag taatgtaagc caataaatca atgcatacgg
     8281 attggaattt accatatact gaaaaattgg caataatcca attatcccat tgttcaactg
     8341 ataaatccag aataaggatt ctacaaataa aaaagtaaac agaaatacta agctcatgtt
     8401 tgatttttta aaccaagaaa aactgctaat agtaagtaag gataaaaaga aatagtatgc
     8461 tatataagag aaaaaaaatc ctataaagaa actagcattg tttgcaatac ttataccata
     8521 aaattctctt aaaaaatcaa cctcctttat ctccaaagag aagctaaaac cattactaaa
     8581 tgcaattaga aaaatcaata aaaataaagt cgccgtccaa atccccgtac taagagctgc
     8641 taatttgaaa ctaaaactgg taaagtgctt aattgatttc agacgaatgc gacactccaa
     8701 cctattaaaa tagttattca tcaaataaaa aaagaacaat atatatgtga acggaaagca
     8761 atatactcca gtcgtcatat cttgaagtaa aactaaaatc cattctaata catttggatg
     8821 gattgaatat tggcgacagc gcaataaata tactgtacta gataaaacac aggatagcag
     8881 taatataaaa taaaccaata ctgataaaaa atcttttttg taaaattgaa caaattgttt
     8941 cattatacat agtcctctga atgtagaaaa aatgtaccat aaacaaccaa acaactaaca
     9001 aataaaataa aagcaagatg cccactaact aaggaaagac tgatatcttt ctgatatccc
     9061 aaagctaatg ttgtcacagg ttctaagtaa gatagcccta aaatagccca aaaaatacca
     9121 ccaaccatca tataggcaac tgggatgaaa atagctccta tttttttctt cactagcaaa
     9181 gcactagcta gtccaaaaat agagaacaca gcgccccaaa ctccatacca gagagtcgtc
     9241 acaagactat agagcaactg attagaatca aataattctt ttaaggcacc actataatct
     9301 ccaatataaa tttcctgata aggagtcact aaaagattaa ttcctaataa taataaatag
     9361 gggagaaaaa agactagaaa agaagaaata attgcagtac tacctacaat agccagatac
     9421 ttctttttag aaattcggca caattgtgct gttagaaaat gactctcagc atcctctatt
     9481 atctgactag aatagggcag tgtacagata agtgcagcta ctaggctaat cggtgaaaat
     9541 cctcgaatag aagaagcggc aaaaaaaatc gataaacctt caattttata aataccattg
     9601 aaagcaagga aatttcctaa actcatgcaa agaagggtca aaaataaaga catataaaat
     9661 cgaggtgatt gaacgactcg tacaagatta caaatgaaaa attccatcct tactcctcct
     9721 tataataaaa atagggtgta gcattctttt ttcatgctac acccacaatc aaccatcttt
     9781 aaggcttact ctgacaagta agttaataag aatctggact ccaagaacct gaagtatgaa
     9841 ttcttacatg atttccaaat tgtggcgcca tagctaatct agtaccagaa ccaatataat
     9901 tgtcaccacc tccattatag tacatgacaa tcctagagcc agaccccaat gaatataccg
     9961 gggtaatatc tgacccacta taggcgctac gaatagaggt atttaacctt ttaccgccac
    10021 cagtgctgtc actattatta attccagcag aggcgttttc tttctcaagc tcactagttg
    10081 tatccgaacc ccaacgcggc aacgttaaat cataatttcc aatctgtcta gctaaaactg
    10141 tagggagaaa caaactagat gcaattgtaa ctacgcaaaa aaggaatata tttttcttct
    10201 tttcatgaca tcgtcctctt aagaagtgat aaacaagata gttgattgat caatttgata
    10261 catattacat tttcattata tcaataaaag aaagcgttgt caattatttt atttaaaata
    10321 ttgtcatatt ttaaataaaa tggtaaaaga gactcggtag cctcgtctct ctttatctta
    10381 ctgacctaac cttcttgcca attttcttgg tcttctttaa agcgttttag aagatcaagg
    10441 ccctctggcg tgatgtaacc ttcttcttgg gctagatgga taagctctct atagtttgaa
    10501 agcgtcacaa gtttaacacc agcatctgca aagttcttat ctgcttttgg caattggtag
    10561 ctgaaaatcg ctacaactcc aagtacatct gctccttctc gcttggctgc tgctacagct
    10621 tcaagaactg aaccacccgt tgaaataagg tcttcaacca ctaccatttt ttgaccttga
    10681 gctacgcgac cttcgatttg attaccagct ccgtggtctt ttggtttgct acggatgtag
    10741 gcaaaaggca agtccatctt atcagcaata atggctccgt gtggaatccc tgctgttgca
    10801 gttcctgcaa tcacttctac ttcaggaaag gcttctttaa tagcttccac aaaaccattt
    10861 tcaattaggg tacgagtttc tggataggct agtgtcacac gattatcagt gtaaatcggt
    10921 gacttgatac cagatgccca agtgaagggt tcctctggtt tgaggtaaac ggcttggatt
    10981 ttcaagaggt ggctagcgat atctttagca agtgtcatgg tattctcctt ttatttttct
    11041 aatctagttc tttaattcca gttctgtgtc cattcatcct tgatggcatg ataagctgca
    11101 acaggatcct cagcttgggt aatgggacgt cccactacga tatagtcact gccgatttga
    11161 taggcatcag caggtgtcat gactcgtttt tgatctccaa ctgcaacacc agctggacga
    11221 atccctggtg tcagacagat aaaatctgga ttggtagcct gcttgatgac ttgtacttcc
    11281 tgagccgagc aaacaacacc atccaagcca gcttcagctg tcttcttggc atagtgaatc
    11341 acagactctt gcagactggt ttggatattt tgaaactcct gcatctgagc ttctgacgtt
    11401 gatgtgagct gagttacagc gatcaatttg gcttgactcc caagaccttc acgcgccgcc
    11461 ttcatcatct ctacaccacc agccgcatgg acattagtca tatcgacacc aagctgagac
    11521 aagaccttca tggctgactt gactgtatta ggaatgtcat gaagtttgag atccaaaaag
    11581 acactatgac ccaaaccttt taagtaggac acaatctcag gccccgctgc gtaataaagc
    11641 tccatcccta ccttgagata aaggctttct tctgctggga aaagagctaa aaattccttg
    11701 accgcctcaa aactaggaaa atcaagagca atgattggac gatgttctcg cataggtttc
    11761 tccttttacc tttactagtg agagagtctg gccaacagaa gccacgaaaa aaggaacctg
    11821 ccaacagcaa ggttccacga aaaatgggta gtctccaccc tttcaccgtc taaccttagc
    11881 tgcctctctg gactgcttta aaggttttct attattctat tatttctact agcacttgtc
    11941 aagtaaaaac ttagtcacgt atgtgttgac tgctattgaa cacctcacaa gactctttcc
    12001 aaaagaatag ttaaagactt tcacttagga cagagaaaaa gagtttgctt gttacccttt
    12061 aattgaacaa atgggaattc tgttttattt tgcggatgcc tattcatctt ggcaaagggg
    12121 aagtaatgag aatgcgaatg gacttcttcg tgagtatttt cttaaaccga tttggcaaca
    12181 attaccgaaa aaatccttaa tggtgctttg ctagcttatt aatcaccgtc cccaaaaata
    12241 cttggattac aagacaggtt ttgaagcact tatagaggaa atttaaaaat gttgcatttt
    12301 ttattgcaat ttatcagtta tcttatcacc caaaacttct gcaattagta tgagcactat
    12361 tgttattttt cttttttcgt acactcaaat caaaatatag caataagact attctgtaga
    12421 attgctgtca aaaagatcag ttcatttttc cttcccactt gtgtttataa tcattaaaat
    12481 aactcaatta agaaattttc tttttcctaa tagttatcgt aattaaagtt aatagaagaa
    12541 caatccaaat aagtagtgtt agattaggaa ttagagtttt attccatgaa aatccctccc
    12601 attttgtgaa aaacttagta aacagtccca taaaactata atctgttatc ttcgcaactg
    12661 tcttatccaa accagatagc atcggtaaga aagcaactaa aatcatagca ggaagactga
    12721 taacctgagc tactacttgg ctcttcgcgg ttaaacctat caataaatag aataaaatga
    12781 tggataaaga ggttagaaga acgattgtaa tataattaaa agtatgtaca attgtaactc
    12841 ctaaaataag aggagtagtt cccataatca caaaagttag caaaaaagga agaaacatcg
    12901 ttgataaaat gtattcggat cctttaacac cactcaacag aagagtttgt aaattgtact
    12961 tttctttttc ttcagacaag ataatagtta taggacttcc aacagccaaa gccaaagaaa
    13021 aaggtaaaca catcatcaaa agaactaatg cctgttgatc gttgaccttc ccctgtgttt
    13081 ccataagata tttataaaaa tatgtgaaag caaaaggcac taaaacttgc aataaaatac
    13141 tcttattgga aaggataatt tgacaacgca accataccaa agccagaaac cgtttaagca
    13201 tttagctttc ctcctgttaa tgtgataaaa atatcttcta aagtaggctc acatgaatga
    13261 attgaaaaaa tattttccag atcagcctga gatacctgtt ctagtgatgt aaaatcaaaa
    13321 gttatctgat tcccattata atctgtaacc ttaatctttt tatctttatt atatctttga
    13381 atgagttcag aaggagctcc ttgctcaact aatttccctt tatttaataa ggcaacataa
    13441 tcacataaaa gagttgcttc attcatatca tgagtcgtta gaaaaatcgt tgtccctgct
    13501 gttttcaatt ctaaaattaa ctcatgaatt gttcgagaag ttgtgggatc tagacctgag
    13561 gtcggttcat ccagaaagag tacagcgggt ttgttgataa gagctcgtgc taaaagcatt
    13621 cgttgcctca ttccagtgga taattttcct gctaccatct tgcgactatc atataatcct
    13681 actcgcttta acaaattatc aacacgtgat ttactaatat tataaaattt actataaaaa
    13741 agaagattgt tatacagaga cattttctca taaaatccac ttgtatcgct aaccaatcca
    13801 attctcttta attcaccgct tgttaaattt tgagattttt gtcccaaaat aatagattgt
    13861 cctttatcgg caaggaactg cccagtcaga atattaatcg ttgtggtctt tccagaacca
    13921 gatggtccta aaaatctaac cttctttaac agtgaagctt atattattta aagctatttt
    13981 atcaccaaaa ctttttgata aatggctaac ttcaatcata ctcattgcac ctcctttata
    14041 ttctacattt ttacttaaca tctatttact tatctttatc aaataaacgt cttaaaaaaa
    14101 acaataacat taagcatata accgttagca agattgtttc aatattattg ataatacgat
    14161 acattctcaa gcagtataaa ataattatat atatgcaaat gctcattata cctatttttt
    14221 taaacatact aagcctcctt gctaaataat aaagttatat aataactaag acacactata
    14281 cctaacataa taaatatcca ctggtaactg aataaatgtt ctaaaattaa caagcaaaaa
    14341 taaaatctca aacgatgaaa aaagccatac tatattttcc aatgatgttt gaatattttt
    14401 attgtagtaa gcataatata tagcaaaaat aatacccaag aacaatacta ttaattgtgt
    14461 ttctataaca gtgaactgcc caatagcaga taaaaaagct actcctcctg ataataagca
    14521 aaaacaaact gaacgaataa atgttttcgc tctaatattg ttatctttca ctttattatt
    14581 tttttacaaa ttggacattc tccgttttcc agaacatatt tttcttgact tagagcatga
    14641 tttctcatat tataaacgta agcgttatct attaggattt cattataaca tcccagaaat
    14701 attttaatta ttaaattagt cattatccct cccaaaatag caggcatctc tggtgtagtc
    14761 ggccactttg tcttatcaga taaagtgtac ttatttatat ctttgtttat attccgacaa
    14821 ccaaagcaag aaccgatggt agggatatat attatcagta ataagatagc atccattaaa
    14881 ccctcctgat atgtaggata tcttatgcga tacagcaaat tgattgacaa tttttataat
    14941 atcaatgggc gtatcaattg ctttaacgat aaaatctata ctcccatatt ccgcaacaat
    15001 tttttctaat tcttctactg aagaaacttt aatagaaata ggtactacct gaacatctga
    15061 attatacttg tgtatttttt cagaaagaac attaatcttc tccttaccaa catccgactc
    15121 tgtataaaga atttgcctat ttaaatttga tggttctatt atatcgtaat cagcgacaat
    15181 aagtttcctc actccataca tacttagact tagagatata taacatccta aggctcctgc
    15241 tcctaatact aatactgttt tattctgtat tttattatac tcttcattat aaaataacga
    15301 atagtaacct ttagttctag aatatctact atcaatatct atctcatccg aaattaaaat
    15361 ttgttcattt aatagttcaa atgttagttt actgcctaat acttcttcta tctctaatag
    15421 ttccttacaa ctattaaata gatcgttgat aaattcttgc gaaatttgag gttctatagc
    15481 tatttcccgc atactccgac cacgcaaata cattatacct tcttttttat aagcaaaaac
    15541 ataactattt acgtagaaca tatttcgtcc ttcctctcat atataatata gaaagcagta
    15601 tagaaacaac cacccctcct atactacgaa caataatatt aggtgtcaaa aactgtaata
    15661 gttgactcaa agggaaataa ataataacaa atgctattaa aagttgaact atatattgaa
    15721 aattcaagtt attattgtta gaattcaagt atcttaaaat aataccacta tcatactgaa
    15781 aaaaaggaat tgaattaata aaaatcatac ctaagttaat gataaaaaat aagagtccaa
    15841 gcaatttata cgatgtgaac aataaaacaa gtaaacctgt aagtgataaa atcgaatttg
    15901 aagcaattcc tgctaacata attttcaaaa attgtttttt attttttaag aattgcaccc
    15961 cacttaaatc tacaaaaaaa agccaaatta aacaaaagta gcattattcc acaatttggg
    16021 atctcaattt gataattatt agcaattatt gcatgactaa attcatgtat cgttgcagac
    16081 ataataacaa caagcaacca aaataaaaga atgtttagcg ttattggaaa gttttggaaa
    16141 ttagtcaaaa tatccgccct ataaatatac ataataaaac attgcaaaat aagagccaat
    16201 gggaaaatac caaataggat tttttcaaaa ttcttattaa atcttttata gtttaatttt
    16261 tcagataatg ttatattaaa gtcccaagat attttaaaaa tatttatctt ttttcgtgat
    16321 ttcatagaat ctataagtcc cattttacta agaacaatat aaaatcttaa ataatatttc
    16381 ttccctacta acactaataa gtccgatatg gtaaattctt gacctattag ttgctctaac
    16441 catttttttc ctgtttccca actaatacat gtttcttact ggatggtata tatagatgat
    16501 actgtccctc ataaggagta ggtaatatct taatcttatc aacaattctc ataacaaacc
    16561 taacctttaa taaataaaag aggggctaag taaaacatac tctagtccct cagtccgcaa
    16621 tcaataaaat aatgactaat tctattaatc tcttgctgca agactaatac ggaaaattca
    16681 aatgactgtc taaaatgaaa ttgaaaaagc ttgatgttag taaatgaaca aacttcaaca
    16741 accagcaaga gcaaggattg ctaaaccaaa tccgatacct gttgcaagtc cagctgaaaa
    16801 atcactaact ggtactgaaa tttcttcaat ttcctcaaat aaaaagttat cttgcatgat
    16861 tatcactccc tatcttttta acaacaggct attccaatta agagaactac cccagttcca
    16921 agtaaaactc ccccaaaaat tcaccatctg taggggctac gatttcttca atttcttcaa
    16981 ataagaatgc ttcatctgac atatgtgtta ccttcttcat aaaaaattat tttgtaatcg
    17041 attacattgc aaatgtaaca taaagaaaaa cagatgtcaa atattaaacg taagaacatg
    17101 gtcactaaag aactataaga gaaaaggtaa acctagcgac gcgatgagcg ctgggtcgtt
    17161 tgatttcgat tgctctcttc ctcttgtttt ttctgttctt cttcctgttt tttctcggct
    17221 tccttggcct cttgtttggc tttttcctca gcctccataa ttaatttatc cgccacagtg
    17281 taactgtaaa ttccagcttc catgtcgacc acactcggtt ctgacaattg tggcttaatc
    17341 ttactgtaat atggcagttt cttactcatt tcagatagag gaaccaagac ttcgtccgaa
    17401 tcattcatgg tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt
    17461 tggatagccg ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt
    17521 tgttcactat cattaaagag aactgataaa taagtttctg gtaaactgtt cagactcaca
    17581 gaactagtct caagctgacc actggaaaga ataggataat gattttcacc agaaatatag
    17641 taggccacaa tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca
    17701 agttgagctg attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct
    17761 agcagaaggt taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca
    17821 gctgtagttt gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc
    17881 aagtaggcag agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag
    17941 atatggatac caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagaaagc
    18001 tttttatcct gttcctcctt ctctttagac tctggttctt ctttctcttc tttctctttg
    18061 tcagcctctg aggatgctac tttttcttca gactcttcct tagctgattc tgaatcttcc
    18121 tggtctgttt cactctcctg gtcctgtttg tcctctgact tctcagattc ttctcccatt
    18181 cgagcttgtc tttccttttc cttctcctca gctagagccg cctcttcttc agccttcttt
    18241 tttagatatt cttggtttcg tttctgccat tctgataact ctttcaattc ttcgaggatt
    18301 tctttgtcct catttttctt atcttttgac atttactttc cttatgataa atcttttttc
    18361 aacaattgat aaaaatctgc tagagatttc aattccttag aagccttcat cttagcttgg
    18421 taatcttcct tgtgacttag taagtgagaa agcttctctt ccaaactatc caaggtcaaa
    18481 tcgctttctt gaaggtcttc tgcatagcct ttttttacaa agtaagctgc attttcaagc
    18541 tggtcaccac gactagcttc acgaccaagc ggcacaatga catgcaattt tgctatcgcc
    18601 aagagctcaa aaatcgtatt ggcaccacct cgtgtcacaa caatatcagc caattccatc
    18661 aagggttgat agagatcggt cacatagtca acacgaaaaa gattttggct caactcattc
    18721 aggctagaat ctccagttag attgataata ttgtagcgct ctgttagttc tttcttatgg
    18781 tctgtcacca attggttaaa gacacgagca cctgcagaac cgccaacaaa caatacagtc
    18841 ggcaatttat gattaaagtg gctttgaata tccaccaatt catctggttc tggatttttt
    18901 tgatctgaaa ccttggccac tgctcccaca tgctcaacct tagacaaact cgaagcttgc
    18961 tcaaaggttg aatacatctt agtcgcaaat ttataggcga ttttattggc caagcccata
    19021 gacaggtcag attcgtgaat aaagacaggc actcctgaca cacgcgcagc gataacaggc
    19081 ggtactgaga caaagccccc ctttgaaaaa agggtctgtg gacgaagtcg caacatgata
    19141 aagagcgatt ggacgattcc ccagcaaact ttgaagacgt ccagcatatt ttgccaagag
    19201 aaatagcgac gcaattttcc agtcgcaata gaatggaagg tgacatccaa acctgactta
    19261 aggatttctt ggtgttcgat accacgcttg tccccgatat agtggacttc ccaaccatct
    19321 tcgatgaact tgggcattaa caaaagattg agggtaacgt gtccaaccgt ccccccacct
    19381 gtaaagacaa tttttttcat attattcttt taactccgct actgtgtcga taaagaggtc
    19441 gccacgtact tcaaagttag catacatatc ccagctggca ttggcaggac taagaagaac
    19501 cacatctcct tgagtcgcaa gctcataggc cttgcgggtc gcatctgcaa tatctgtcgc
    19561 ctccacataa gcgacaccag ccttgtctgc tgcccgtttg acacgttctg cagattgacc
    19621 caggatgacc atcttcttga gtccagtaat gtctggcacc aattcgtcaa actcattgcc
    19681 acggtccaaa ccacctgcaa tcaagacgac cttgctgtta tcaaatcctg acaaggcttt
    19741 ttgagtagcc aagatattag ttgatttact gtcgttatag aatttaacac ccttgatgtc
    19801 atccacaaac tggagacggt gtttgacacc accgaaggct gaaagagttt ccttgatggt
    19861 ttgattgtcc acaccacgaa gcttggctac agcaatagtc gcaagggcat tttccacatt
    19921 gtggctacct ggaacaccga tttcattcgc tgccatgact acttcaccac ggaagtagag
    19981 ttgaccatct tccagataag ctccatcaac cttttcaagt gttgaaaatg gtacaacagt
    20041 ggcttctgtc ttggaagtca agtcttttgc caagtcttga ttaaagttca agacaaggaa
    20101 atcagctgct gtcatcttgt tctggatatt ccacttggct gctacatatt ccgaaaatga
    20161 cccatggtag tcgatatgag ttggcatgag gttggtaata accgcaatct ctggatggaa
    20221 ttcttgaaca cccatgagtt ggaaagaaga aagttccata acaagcgtgt ccttatctga
    20281 tgctatttga gcaacctgac tagctggata gccgatattc cctgataaaa gaccatgttg
    20341 cccagcagca gtcaaaactt ccccaatcat agtcgttgtg gttgtcttac cgttcgatcc
    20401 tgtgatacca ataatcggtg cttctgaaat caaataagcc aattccacct cagtcaagac
    20461 tggaattccc ttggccaaag ccttttcaat catgggattg ttgtagggga tacctggatt
    20521 tttcaccata agggcaaact cttcatccaa gagttccaaa ggatggccac ctgtaatgac
    20581 cttgatccct tcttccagca aactttgggc agctggattg tcctcgaaag gtttcccatc
    20641 atttactgtc acaatggcac ctagcttgtc caacaaacga gctgcagatt caccagactt
    20701 ggccaaacct aaaacaagga ctttcttatt tttaaattga tctattactt tcatgtctcg
    20761 aactccattt ctactcctac tattttacca tttttatgga aataaaaaag ccacaaagtg
    20821 tgtttgtgac tctttcttct aactgaatct taccatatca tctatgtgat aaatcggtaa
    20881 ctcgaatgac ctgatccact tgttcccaaa taagaggatt gtgggtcgca ataataatgg
    20941 tccgattcgg attttttaaa gattctagga tggaaagtaa ttcctcagag tttttggggt
    21001 ctaaggaagt ggttggttca tctgcgagga tcaaaggtgg atcctttaaa attatcttcg
    21061 ctaatgcaac acgttgtgct tctcctcctg ataactcaaa tataggttgc ttcaaatcca
    21121 aataagagag gtttacacag tttagagctt gtttcatcaa agagattttc tctttttcct
    21181 tcaacttttt accaactaaa cccagattga gattctcttt gacggtttgg ctttcaatta
    21241 agccaaaatc ttgaaataag tatcccaagt aatctctaaa gaaaacagaa ggcttgatgt
    21301 ccttaagaga agtaccatca tagatgattt gtcctttgtc atatggctct aatcgtccaa
    21361 tcatattcaa gaatgttgtc ttaccacagc cacttgtacc gattaaggca taaattttcc
    21421 caccttcaaa atgaagattt gtatctgaaa atagctgacg gcttccaaat tttttagata
    21481 tattctttag ttcaatcatc ctattttcct ttcataattg tcatagaaac acgagattct
    21541 ttctgcgctt gacggtaaag cgtcagaact gcactagcta gaaagactaa taaagtgagc
    21601 aagccaatca ccaagtctcg actgcttaaa ataaagagac tagcaccaaa tacaaaactg
    21661 gcaaattgac taaccatata ctgagcatgt gtttcaaaaa atcgtaaacc tgaaattcgt
    21721 ttaatcaaaa tatctcggcg gaattgctcg aaatatagaa gattgacaga ataaaatagt
    21781 aataaggaac tagctatccc cacaatagct cctaagatta aagttgctgt ttcagtttga
    21841 acttcattat aacgagttag ataaacactt cttccttctt taagatagga tacttgctca
    21901 taaattccag ctttcttcaa gagttctagc ccactctcat atcctttgat aaagagttgt
    21961 tttccagcat tgatagacca actagataag gatataaaac tatcacctgt agaagtcggc
    22021 gtgaatacca ctaaaatcgg atcagtcaaa tactgagtag atacgggatt ctcaccgtta
    22081 ttataaacaa accgcttttc tcccattgaa agataactaa cgtgcgcttt catctcataa
    22141 tccaaaggag cacttgcctc ctcaccagat tttccataat aactcaatct ttcttcaaaa
    22201 actttcttaa gttctgcttc tcgagagcgc aaatgttctg ggagcaagag gataaactca
    22261 cctttttgga gatgggctaa cttctgtttg gtctcagcat ctaccacgac cttttccttg
    22321 tccaaataac tgggactaac atagagcgta ttagcatctg gactataggt atccagtgtc
    22381 tctccctgtt cattttttcc ttgtggattg gcaaaatgga gcagattatc ctttacataa
    22441 agagcttgtt cttcttcgat tgcttccttg gcaaaggcat accacttgct ctgattttct
    22501 gtatcttttc ctctatcacc taagccaaaa gaaatttgat aatagtctgc tctatcctgc
    22561 catgcttgtt ttgaaatttc aagttctttc aatcgttggt aagacatcaa acctgtctta
    22621 acagcgtagc ctactgtaaa gacagcaacc aactgacaca atagggttaa aatcatcagg
    22681 cgtttaaggg gtaatctccc cttaataacg ggaactaatg ctttgtaact caaactcatt
    22741 agataaagaa gcattagtaa aattgaaatc gccaataaaa acaacagata gaaactaatc
    22801 ccaaaaccat aggtggctaa caagatagga taaaacaaac cttgactaaa aagaacgact
    22861 cccccaccta ggaaggaaag gagggctgat agaaggagcc atttgatatc agtagataaa
    22921 gaatgcccca tgatggataa gagagtctga ccagaaaaga gttttatacc tgctgctctc
    22981 atttccttaa tccgagtgat aatcactaaa gcaaagaaag ataagccaaa tattgctaaa
    23041 ctaattaaaa taaggggatt tagtaatatt cgaaaagcaa gaaaataggg cggtatcttt
    23101 cggtcagcac ttgctttata acccaaatct cctaatttat cggcaagctt ttctttcgtc
    23161 aaggagcctg acaaaaggag ataactattt agcggatcac tacgttcacg actttcttgg
    23221 ctagcttctt ggaattcttt tggtaaagtt ccctgaccat aagttgcata agtaaagtga
    23281 gtcgtcccat ccttactcgg ctctacaatt cttctagcta ttaaactctg ttctgagttt
    23341 gcaaaattct ccaattcctg ttcaaatacc tcacgcgtcg gttcctgagt atcttttttg
    23401 acacgaagta aagaaacgga atcatagctt gcatataaat attgtggcgc acgtaagaca
    23461 ataatccaag caaggaagaa gctgagaaaa aaagttgata ataatatgaa tagtttcttc
    23521 atagtagact ccttgtaaac aaaattcccc ctgtaatttc ttacaagggg aacgatttaa
    23581 atcaatgaac gattagtcat aatcacagta aaatgctact tgttctcccc atttagtcca
    23641 aatccatgca taggaggttt ttccagcaga agcttctcct ttatctgaat gactattcca
    23701 tctacttaca agagaagaat aatgatattt tttaccatga tagtaatttg aaaaagccca
    23761 accacctcct gaaccttctc catacgtcca tactcctcca tctggatatt gtacagcagc
    23821 tgatgcagct cccaataatg taaaacttga aataagagct agagcaagta atctatgttt
    23881 tttcgttttc attttatttt ttctttcaaa aaaagcacac cttgagcaac aatgcaacaa
    23941 aataaaccct cctctctctt ttgttgaaac cgctttctta tgtgataaaa atatcatttt
    24001 tattatttgt tgtcaaggaa aaaatcgaat tttttagata ttttactaca ttgcctctat
    24061 gaataatatt atatagtagt tttattttaa aataatatgc aaccagtact aaccaaatat
    24121 aaaatagatg ccattaacga attttattca agtttttccc attcatacta tccaagcaaa
    24181 agagatgatg ttatagtaca aaacaattca aactattgta aaattcctag caaaaaagag
    24241 agccaaaact ctctttttta tcttctttta ctttttttga ctggcatgag tgtaatgtct
    24301 ctaacactaa agtaagctag gatcaacatg gctattgcta ggaatatttc tgttggtaat
    24361 tgaaaaattt tcagaaaaga tagaaccaat aaaatcaaga gtgccactaa aatacatacc
    24421 atagcgacga tattgactgt ccctttaatg ctttctggtg tcgcaaatac atagagtagg
    24481 agcagtaaaa ttcctaggac taaatagacc atctttctct ctttctagct cttattcagc
    24541 tgattttttc ttcttgttag ctttctcacg ctctgctttg ttaaggattt gtttacgcaa
    24601 acggatagac tcaggcgtta cttccatgta ctcatcgtcg ttcaagaact caagagactc
    24661 ttcaagtgtc aagatacgag gcgtcttgat aacagctgtt tggtccttag tagctgaacg
    24721 aacgttggtc atttgttttg ccttcgtgat gttaactgtc aagtcatttt cacgagagtt
    24781 ttcaccgatg atcattcctt cataaacctc agtacctggg ttgacaaaga tcgtaccacg
    24841 ttcttcgata gacatgattg agtaagttgt agccttacca gcatcgatag aaacaagggc
    24901 accacggtga cgtccaccaa tttcccctgg aatcaatggc aagtattggt cgaaggtatg
    24961 gttcatgata ccgtaaccac gagtcattga caagaactca gttgagtatc caatcaaacc
    25021 acgcgctgga acaaggaaga ccaaacgagt ttgaccatta ccagttgaaa tcatatccaa
    25081 catttcacct ttacgttcag aaaggctttg gataacagac ccttggtatt cttctggagt
    25141 gtcgatttgt acacgttcaa atggttcaca tttaacaccg tcgatttctt ttacgataac
    25201 ttctggacga gatacttgaa gttcatagcc ctcacgacgc attgtttcga taaggattga
    25261 caagtgcaat tctccacgtc ctgaaacagt ccatttatct ggtgaatcag ttgggtcaac
    25321 acgaagggaa acgtctgttt gcaattctgc ctgcaagcgt tcttccacct tacgagaagt
    25381 tacccattta ccttctttac cagcaaatgg tgagttgttg accaagaaag tcatttgaag
    25441 agttggctca tcgatgtgta ggattggaag agcttctact gcatctgtcg gagtgatggt
    25501 ttcaccgaca aagatgtctt ccatacctga aacggcaatc aagtcacccg ctttggcttc
    25561 ttggatttca cgacgttcca aaccaaagaa accgaagagt tttgtaacac ggaagttttt
    25621 agttgtaccg tcaagtttag aaagggtaac ttggtcccca accttaactg taccacggaa
    25681 gacacgaccg ataccgatac gtccaacgaa gtcattgtag tccaaaagtg acacttggaa
    25741 ctgcaaaggc tcatctgagt tatctactgg agctgggata tggtcgataa tcgtgtcaaa
    25801 gattggtgcc atagtcgctt cttggtcagc tggatcatct gacaatgaag aagttccgtt
    25861 gatcgctgaa gcataaacca ctgggaaatc aagctggtcg tcatctgcac caagctcgat
    25921 gaaaagttcc aagacttcat ccactacttc tgctggacga gctgatggct tatcgatttt
    25981 gttaacaacc acgattggga caaggtcttg ttccaaggct tttttcaata cgaaacgagt
    26041 ttgtggcatg gttccttcat aggcatctac gaccaagaca acaccgtcaa ccattttcat
    26101 gatacgctca acttctccac caaagtccgc gtgtcctggt gtgtccataa tgttgatacg
    26161 agttccgttg taagcaacgg cagtattttt agcaaggatg gtaatcccac gctctttttc
    26221 gatatcgttt gagtccatag cacgctctgc caattcagta cgtgcatcaa gcgtttctga
    26281 ttgtttcaat aattcgtcaa ccagggttgt tttaccgtgg tcaacgtggg cgataatcgc
    26341 aatgttacgg atatcttctc ttaattttgt catgatttcc tctataatat tcaaaattta
    26401 ttttctaact gaacgattat accataattt caaataaata acataactca agcaagtgta
    26461 aatgttttca ctccgctttt cttttcacgt caagcctttt caaagcaagc gacttatgat
    26521 aagataggca cagtatgcgt ttagataatt tattagccca agaaaaagtt agccgaaagg
    26581 ccatgaagca agcactcctc agaggggaaa ttctagtcga tggttgccca gcccgatccc
    26641 tagctcaaaa tatcgataca ggactacaag aactcctttt tcaggatcga atcattcaag
    26701 gctatgaaca cacctatctt atgcttcata agcctgctgg tgtcgttaca gccaacaaag
    26761 acaaggaact tccgaccgtc atggacctgc ttccatctga catccagtct gacaagctct
    26821 atgccgttgg ccgactggac cgagatacaa cgggactcct cctcttgacc gataacggtc
    26881 ccttgggctt tcagctcctc catccccaat atcatgtcga taagacttac caagtccagg
    26941 ttaatgaact tctaacacct gaccatatcc aagcctttca aaagggaatt gtctttttag
    27001 atggtactgt ctgtaaaccc gcaaaactag agattctatc tgcaagtccc tccctcagtc
    27061 aagcctctat caccatttca gaaggaaaat ttcatcaaat caagaaaatg ttcctctcgg
    27121 ttggtgttaa ggtgactagc ctcaaaagaa tccaatttgg ggacttcaca ttgaacccag
    27181 atttagcaga aagtaactac cgccctttga accaaaaaga gttacaaatc attaaaaact
    27241 atttagagat gagtcgataa aacaaaaaaa gctttaaaac taaagctttt ttcttttatt
    27301 taccgaaaaa ttaaggcgat tgctacaatc cagttaacta cagaaatcac aattcctaag
    27361 atattaagaa tcttttctat tttatagtct aattgtgact ctttttggta tgaaatagcc
    27421 aagaccaatc ctatgatacc caaaatcaag cctacaattg gaaataacaa accaagaata
    27481 atcgacaaga tacccacaaa aagtggattt ttcttctttt ctttcatgtt ctaagaactc
    27541 tttaaatttt atacaaatta attatactat aaaacaatag cttcatccta tcattcgact
    27601 aatttggaaa taaggttagc tagtcttcac tttccctttc caagaatcca agccataaga
    27661 aaggatataa atctcagaaa aaccttgttt tttcaagtaa agagctgcat ttgtaactcg
    27721 ttgcgcacgt tggttctcgt agagaaggac aggtttatct ttacgaaggg ctgcaagact
    27781 agttttcaac tgacttgaag gaatattgcg tgcaccaagg atatgttttc tgtggaattc
    27841 tgctgggtcg cgcaaatcaa tcaattgacc cgtacgaatc aaggcttcaa actcctcatt
    27901 gtccacaatt ttagccgcac gacgaatacg aagatagtta aagcccatcc acgccaacat
    27961 tgctagtata agtgcccaca aaatccaagt aaccattagt tcttttctcc atttttctca
    28021 atataatcca attctacctt gtgctctctg cgaagaactg cttctgcctc tagatagtct
    28081 aatttatcca tcaaccctgc atcgtaaatc cgagatagtt ccaacttcat cagttcaata
    28141 tcatataagc gttttcccat gtaaacaata ataccaaatc gtttgaggaa ttgctgcaca
    28201 tcatagaatg ttttcataag actcattcta gcaaaatttt gtgttttttt caagaagaga
    28261 ctcacacaat gctccttatt ttcctatctt ctttggcgat tctaaggcaa gtgtggtaca
    28321 ataaaaacat gagaattcaa caattacatt atattatcaa aatcgtcgaa actggctcta
    28381 tgaatgaggc agccaagcaa ctctttatca ctcagccaag tctttccaat gctgtgagag
    28441 atttggaaaa tgaaatgggc attgagatct ttatccgcaa tcccaaggga atcaccttga
    28501 cccgtgatgg gatggagttt ctctcttatg cccgtcaggt tgtcgagcaa actcagcttc
    28561 tggaggaacg ctataaaaat cctgtcgccc accgcgaact ctttagcgtt tcgtctcaac
    28621 actatgcctt tgtggtcaat gcctttgtct ctttgctcaa gaaaagcgat atggagaaat
    28681 acgagctttt ccttcgtgaa actcggactt gggagattat cgacgacgtc aagaacttcc
    28741 gcagtgaggt cggggtcctc ttcttaaaca gttacaaccg tgatgtttta accaagatgc
    28801 tggatgacaa tcacctacta gcccaccatc tcttcacagc gcaaccgcat atctttgtca
    28861 gcaagaccaa ccctctggca aagaaagaca aggtgaaact gtctgatttg gagaatttcc
    28921 cttacctcag ctatgaccaa gggacgcaca actccttcta cttttcagaa gagattcttt
    28981 ctcaagaaca ccacaagaaa tccattgtgg tcagtgaccg tgccaccctc tttaatctct
    29041 tgattggttt ggatggttat accattgcga cagggatttt gaacagcaac ctaaacggag
    29101 acaatatcgt ttctatccca ctggatattg atgacccgat tgagctggtc tatatccagc
    29161 atgagaaaac cagcctatct aagatgggcg aacgctttat cgactatcta ctagaagaag
    29221 ttcagtttga tagttgagaa atgataagaa ccaatatgta ggctagcaac aacctgcaca
    29281 ttggttcttt ttacttataa ttaaaagttt cccctgccaa cttatcagct agcttgggaa
    29341 agagagtata aaacttatgg gctaggttca acaaaatcgg gagattgagt tctcgtttat
    29401 tttttcctat aatcttgaca atctttttag ccactgcatc tgcctctaag aggaagcggt
    29461 caaccgattt aagataagtt ccatctgggt cagcttggtc aaaaaatcct gttcggattg
    29521 gacctggatt gactgttgtc acatagactc catagggcat aagttcgagt cgcagagcat
    29581 ttgaaaaacc aatagccgca aacttggtcg ctgagtaaag actagacttg ccagtagcta
    29641 ttagacctgc catgctgacg atgttgatga tatgcccttt gctgctttcc ttcatacgag
    29701 ccgcaaggtg acgagacaga ttcatcaggg caaaggtatt gacctcaaac atctggtgaa
    29761 tatctttatc agcaatctgg tcaaatccct caaaaatccc gtaaccagcg ttgttaatca
    29821 agacatcaat cttgccatag cggagataaa gatcagttac cagagcttct agggctgaat
    29881 cgtcggtaat atcaatttca atcaattctg catgggaata atttccgtag agttgggcta
    29941 atttttcctt atttctacca agcaagatga gttggtcatt gggcaggagt ttgaccattt
    30001 cttgagctag accaccgcta gctccggtaa tgagaatagt aggcatactt atcctttctg
    30061 tgactgctag atttccactt cttccaagtc tttgaccaca tggacatttt caaaaattgt
    30121 ggcagcgtcc ttcttgagtt tgcttatatc ttttgagagg aaacgggcac tgatatggtt
    30181 gagtaggagg cgtttggcac ctgcttctac cgctacttgt gcagcttgca tattagttga
    30241 gtgaccatgg ttacgagcaa ttttttcatc acccttgcca taagtggact catgaactag
    30301 gacatctgca ttgacagcca gacgcacact ggcatccgtt tttcgagtgt ctcctaaaat
    30361 agtgataatc ttacctggac gtggcgctga gatatagtct gctgccttga tttcagttcc
    30421 gtcttccaaa acaagatcct ggccgttttt gattttacca aaaagcgggc cgaacggaac
    30481 accagcagcc ttgagttttt cagcatccag cgtcccttct agatcctttt gcatgacacg
    30541 atagccaaca cagaaaatag tgtggtccag ctcctctgca tacacagtga atttatcggt
    30601 ttcaagaatt ttacccagag aatcttggtc aaactcatgg aaatgaatgc ggtagggcag
    30661 acgagaacct gacacacgaa ggctggttaa gacaaatgac ttgattcctt gaggtccgta
    30721 gatttccaaa tctgtctgct cttcattggc ctgaaaggca cggctagaaa ggaaacctgg
    30781 caaaccaaaa atgtggtctc catgcagatg agtaataaag attttgctga ccttacgtgg
    30841 tcgaattgtg gtttccagaa tgcgattttg cgtaccttct ccacagtcaa agagccaaac
    30901 ttcgttaatc tcgtccaaaa gtttcagggc gagacttgaa acgttgcggg ctttagaggg
    30961 ctgaccagcc cccgttccta aaaattgaat atccattcga tactttctaa ttaatcaata
    31021 tataacatgg ctgtgcggtt ttccgatcgg aaatagcgtt tgccagaaaa agcagcagct
    31081 tcttgcaata aatcctcttg gctgtagcct ttgagacgtt ttcgaccatc agccaatctt
    31141 tccaaatcag tcaaagctgt gagactttct aggctgacaa cttcctcgtc cttgacaggc
    31201 ttcatgtaaa tcttaccaga ctcttcaaag actaattgat gggagaaaat ttgcgcaatt
    31261 tcaaagagca agtcatccga gattttctcc tcattttcag agaaaatccg accaaagccg
    31321 tcactctcat aacaaaaacc aaaggattta ccagacagat taagccgaat aaaaggctta
    31381 ttttctaggg tgaaacttgg ctcagtattg taaagattca gttcctgact gagttctgca
    31441 aaataatccg tcgcagcctg aggacttttt ttctgataga gttctgcaaa gtaggcatta
    31501 acaacacttg gcggaggtgt aataagtgtt aactgctcct gctctgtttt accagctaga
    31561 agctgatcca gatagacctt gtccagactt gtataacctc catactttag agccaaagtt
    31621 ttaatatcag tcataaaatt cttctaacct ccatttattt ttctcggaaa tgtagcctgt
    31681 aatcacttcg ccgtcttcct gataatcacg ttcttccaga attgcaacac tctctaaatc
    31741 atgaatcttg taggactttg aaaaaggcac tcgcagggta aatgcttcaa aaatttcctt
    31801 aatcttatct agcaataatg cttgcaagtt ttcacgactg tcctcagact tggcagaaat
    31861 gagggtatat ggcgtttggg taggcgtgaa atcctccacc aaatccgctt tattataaag
    31921 cgtcaagtga ggaatatctt ccatgtccag gtctttcatg atggagagaa ccgttttttc
    31981 atgctcctcg tggtaaggat tgctagcatc gataacatga accagaaggt ccacatgctt
    32041 gctttcttcc aaggttgact tgaaactgga caccaactct gtcggcaaat cttggataaa
    32101 gccaacagta tctgtcaaag ttacttggag attgcctccc agatgaatac tcttggttgt
    32161 cgcatccaga gtcgcaaaga gctcatctgc ttcatactgg gtcttactgg tcaagatgtt
    32221 catgatagtt gatttcccag cattagtata accaatcaaa ccaatcttaa aagtgctaga
    32281 ctccaaacgt ttttctctga cagtcgcacg atttttctca accaccttaa gctggcgctc
    32341 gatatccgtg atttgattgc gaacgctacg acggttcagc tccagttggc tttcaccagg
    32401 accacgggaa ccaattcccc ctgcctgacg gctgagcata atcccctgac caaccaagcg
    32461 aggcaagagg tatttgagtt gggctaggtg gacttggagc ttcccttcat ggcttcgagc
    32521 ccgcatggca aagatatcca aaatcaactg catacggtca atgaccttaa caccgagaac
    32581 ttcctctaga ttgacattct gccttggggt cagacggttg ttgacgatga cagtagtgat
    32641 ttcttctgca tccaccataa gcgcaatctc ttccaactta ccagagccga cgaaggtctt
    32701 ggaatcatat ttttcacgtt tttgtctgta gctatctaca acgactgccc ctgccgtttt
    32761 cgctaaacta gccaattctt ccatggagag gtcaaaactg tccataccct gcaattccac
    32821 accaatcagc aggactcgct cctctttttt ctccgtttca atcatctaaa aactcctcta
    32881 tctggcttaa aatgcggtct tgtacaccag attctccaat ctgataaaag gtgacctgca
    32941 tgcgattacg gaaccaggtc agctgacgct tggcaaaacg acgggtcgcc tgtttaagac
    33001 tctcacgagc ttcctccaag gtctgctctc cacggaaata aggaaagagt tccttatagc
    33061 caattccttt agcagcctgt acattagggg aatggtcaaa cagccacttg gcctcatcca
    33121 aaagcccagc ctcaaacatc aaatccactc ggtggttgat acgctcataa agttgactac
    33181 gttcatcatc caagcagata atcagcggtt catacaaaat ctcttgattt tccaaatcct
    33241 gaccaaaatg ggcaatttct aaggcacgca tagcacgacg acgattaaac tggggaatct
    33301 caaggcctgc ttgctccacc aaatgggcta attcctcatc tgaatatggc tccaaactag
    33361 ctcgataagc taaaatctcc tcatgaggag tctccccacc taggtggtaa ccttctagca
    33421 agctctggat ataaagtcca gtcccaccgg cgataatggc tagcttgcca cggctgtgaa
    33481 tatcctcaat agtcatctta gcttctgaaa caaaatcaaa agccgagtaa gactcagtta
    33541 tctctctaac atcgattaaa tgatgaggaa cagctgcctg ctcttctgga ctagccttgg
    33601 ccgtcccaat atcaagtcct cgatagactt gctggctatc tccactaacc acttcgccat
    33661 taaagcactt tgcaacttca atagctagag ccgtctttcc aacagcagtc ggtccaacaa
    33721 tcacaattat ttttgttttc atcttttttc cttgaaaaat tcccattttt tcgttactat
    33781 tattataaca caaaaaggtc agtcagaaaa atgtgacctc ttgaggaggc tggctgattg
    33841 agaatataaa ggaggaagac tcatggctaa aggattcgct aaaggtcttg taacaggtgt
    33901 cgcaggaact gtcgctgccg ttgcaggtgc agtatacgca tttaaaaaga aagtaatcga
    33961 accagaagag caaaaagcag ctttcatcga agaaaaccgt aaaaaagcag ctcgtcgccg
    34021 cgtatcacgt taagaaacaa aagaagttgg gattattctc aacttctttt ttctattctt
    34081 ttaaatagct aggtcaaact tcaactgtgg cttaacaggg tcataatcca ccaactcaaa
    34141 atcttctgct ttgatatcaa agaaattagt cccatcagga acatttaaaa ccaagcgtgg
    34201 ttggcagttt gacggctccc gacggagcaa ttcctgagct tgttcaaatt gattatcata
    34261 gatatggagg ttgttgatga agtagaagaa cttcccaacc ttccagccaa aatgtttagc
    34321 aatcatcatc tgcaaagcca catactgcat agcgttgatg tggtgggcca ccagcatatc
    34381 attggagcgc tgggtcaagg tcgcatccag atagatttcc ccatcaacac gccgaacatc
    34441 aaacatggtc tgaaaggcgc acgggagcag cccatctgtt tcttcgaaag cttggtaatc
    34501 ccagagagaa ataatattgc ggcggttcca aggattggtt tccaactgtt tgagaagctt
    34561 attgataatg tcgtgtttct taacaacggc accatagcgc tcaccaatgg ttcccgtgtc
    34621 tccaacttcc cagtcattcc agtagtgaac attgtacttg tcattaagca cttctaagct
    34681 atttgactgg tcttggtaaa tccagagaac ttctttgatg gcggatttga ttgcaatggg
    34741 acgcaaggtt atgataggaa attctccttt agataagtcg tactcggaaa aggcaccagt
    34801 tacatacttg gaattagcaa cagtcccatc cttgtactta ggacgagcct gctctgagaa
    34861 aacaccttcc tttagaatgc gttcaatatt ctctttaaaa atcgtatctg cttttgtcat
    34921 ttactctcac ctcattcatt gtcctaacta gtatagcata aaaaataaaa gaggaacatt
    34981 actaactcta ggttagcatt ttcctctctc attatcttat tgcaatacaa gtgatgctgc
    35041 tccgataact ccagcgtcat ttcctagagt tgcaagagct aatttagtag atgtgcgtac
    35101 ttgtgggaaa gtattttcgt catagacttt ttgaacacct tgtagaagga attctcccgc
    35161 agctgacaca ccaccaccga tgacgattgt tgatgggttt aggattgaac caatgttagc
    35221 acaagcgatt cccaagtaac gtgagaagtt acggtaaaca atcaaagcaa ggtcgtctcc
    35281 ttcttttgcg agatcaaaga cagtcttagc agttacttct tctccgttat caatcaagcg
    35341 tttcaaggct gcatcgcctt cgtattcatc ggcatagcga cgagtcaagt tgacaattcc
    35401 tgttgcagaa gcgactgtct caagacagcc tttcttaccg caagtacatg agattggctg
    35461 gtcaaagtca acagtgatgt gacccaactc acctgctgca ccagcaacac cgtgaagcaa
    35521 tttgccttct gcgacgatac cgccaccaac accagtaccg agtgtcataa agacaacatc
    35581 tggttggtta tcaccagcac ccatccagcg ctcaccaaga gctgctacgt tggcatcatt
    35641 atcgatgaaa aatggaatgc ccaaagcttt ttcaatcttt tgtttgattg gttgaagggt
    35701 tttccagttc aagttgtagg caccgataac agtccctttg tcacggtcaa ccacacctgg
    35761 tgatcccatt ccaatgcctt ggaagtccgc tgctgccaat ccaagcaagt ccaaacgatg
    35821 ttgaatagac tcaatcatat catcaacgat atgacttccc tcatccaaaa tgttggtctt
    35881 gattgaccat tttccttgga tttctcctgc tgttgttaag attgcaaatt tgatagaagt
    35941 tccaccaagg tcaatcccaa taatcttttg actcattata ttctcctttt tcattgtgta
    36001 gtaattgaaa atgcttacag tactagtata tcaaaatcca ttcacttatg caaaggtttg
    36061 cgtaaatgaa ttataaggaa ttttttcgcc taccagtatc tgttaaccat ctaaatgatc
    36121 aatggcatat tgagctgcat catttgaaaa tttatcaaaa tcagaagtta attgtctata
    36181 catacgtttt tttgacatgt ggaataacga attataactt tttgcctttc ctaaagcagc
    36241 agaatattca ctattgctat cattactaga agaagctgta cttgcttgac cttccttcca
    36301 aactccgtca gcgccaacat aataacgtcc accatcaacc cattcattga cagccatttt
    36361 accatccgat tttagatagt aatttccttg ccatgtacta taagcataag aaccatctga
    36421 tgtcaaataa taccaagctt gataggtggc atcataaacc cattcacctt tagccatttt
    36481 accatctgat ttcaaatagt agtttccttg ccatgcattg cgagcatatg aaccatctga
    36541 tttcaagtaa taccatgctt ggtaagaaga atcataaacc cactcacctt gtgccatttt
    36601 accgtttgat ttaaggtaat aagctccttg ccatgcattt tttgcataag aaccatctga
    36661 tgtcaaataa taccaagctt gataagaaga gtcataaatc cattcactct gtgccatttt
    36721 gccatcagct ttcaggtaat agtttccctg ccatgcattt ttcactagat ttccatttcc
    36781 atcaaaatag taccaagaac cgtcctgctt aacccaacca cttgctgtgg atgaagttgt
    36841 atcagtttga gttgtagccg cttgttttgc tttaaaagta cccttaggag catttttcaa
    36901 ttcgcaagcc acaccatcat gatcgcggtc taacttggca gaataaccag gttctccctc
    36961 atgaagatcc gaatatccat tagcccaagc ttccttacag ctcgaaaaat ggatgttttc
    37021 ttccgccaaa actggttgtg aaaacaaggc taaaattggc aacgtcacca gactcatttt
    37081 taaaaataga cgtttattca tttctttctc ccataattat gatatcgatt tcaatattat
    37141 tttatcaaac ttatttcagc aattcaagga aacttctggt cttttctaca aactcctttg
    37201 gttttgcctt attcaagaca tgagggccat caggaatgat ctgaaactgc gaatctgtta
    37261 acagtctatg gagagctttc atagaactaa gattcggttt atctttgctg ccacaaatta
    37321 gtaaggttgg ataggagcaa gtccctgcta tatccgttaa atcaagtgtc ttcaattcct
    37381 cagaaactcc gaccataaga gtcttgtctg ctccctgttt ttcaaatact cttttgggaa
    37441 gtagtttaaa aatcagcaac tgaagataaa ataggatatt ccctgctaat ttaagcgggc
    37501 atcctgacag aatcaaagct cgaagatttg gtaaatcgta actggaaagt tctagtgtca
    37561 gggcagcacc taaggacaat ccaatcaaaa caaaaggttc tgtctcttga gctaggtgct
    37621 gataaactcg ctctttagct tgttgatagt tactaactcc agaaggaaat aactcgatag
    37681 cctcagaagg ataatctgtc agtagattcc gaacttcttt ccaagactct gctgactgcc
    37741 ctaacccatg caaaaatatc aatttcatct agttctcctc aaggcttagt tcatacaagc
    37801 ctctcactgc attacagccg taaatagctt ctgcttgggt taaatctgcc aaggtcaaga
    37861 ctttctcttc tacctgtcct gtttctagca aatactgacg gtaaattcct ggtaagattc
    37921 caagtcggat aggcggtgtg tagagttttc cagcgatttt cagaaccaaa tttcctatag
    37981 aggtttcaag cagttctcct gacttattgt ggtaaatctt ctcttgttct cctaggctca
    38041 aatgcggtcg gtgagtggtt ttaaagtagg taaaggattg attcaaagca gcttcctgaa
    38101 ggcagacttg ggcctgacaa aagcttgtac tgagaggggt taatacttga cgattgactt
    38161 ctatctctcc agatttgcta aggctgattc gcaagcggta atctcgatta gcttcacaat
    38221 cctgacactc ttcctcaatc ttgtgtccca agtcttctgc atcaaaagga aaagcaaaat
    38281 aacgactagc ttttctcagt ctttccagat gttgatcttc aaacagcagg ttcttctggc
    38341 tgatttcccc tgtcgtaatc agttggaagc gagcttgttt acgataaaga actgctgcct
    38401 tttgatgaac ctctcggtat tcagattccc atgtgctatc ccaagtaatc cctccgccaa
    38461 ctccataaat ggcttgacct ttgtgaagtt gaatggtacg aatggccaca ttaaaaatcc
    38521 gtcgtccatt tggaagcaag agaccaatcg ttccacagta gactccacgc ggttgaggct
    38581 ccaagtcctt gataatctcc attgtcgcaa ttttcggtgc acccgttatg gaaccacaag
    38641 gaaagagtga gcggaagatt tcaacaaggt ccacatcctc tcgcaactga ctcttgatgg
    38701 tcgaagtcat ctgccaaaca gttgaatact gctctacctg acacagacgc tccacgtgct
    38761 cgctcccaac ttcagaaata cggttcatat cattgcgcaa gaggtccaca atcatcatat
    38821 tttcagagcg atttttggga tcctgttcca accaactggc ctgttcaaga tcttcttggt
    38881 cagttacccc acgctgagtc gtccccttca ttggtcgtgt tgtcaactcg cgatcatttt
    38941 gctcaaaaaa gagctctggg ctcatggaaa tcactgccat ctcgtcatgt tccacatagg
    39001 cattgtagcc cgcctcctgc tctaccacca tacgattgta gatggcaaaa ggattggcac
    39061 ttaacttttg cttgagttgg acggtgtagt tgacctgata ggtgtctccc tgcctcaagt
    39121 gatggtgtat ctgggcaatg gccttttcat agtctgctgc agacgtcact tcctgccaat
    39181 ttgagggcaa atcaatatcc tcataagtca gaggaatagg ggaagtttct acgctatcat
    39241 gaacagtaaa gtaaagcagg tactctccca gtaggggatc cttgtgaact gctaattttt
    39301 cctcaaaagc aggtgcagcc tcgtagctga cataccccac cacataataa ccttgctctt
    39361 ggtagctttc cacttgtgcc agcaaatctg ccacttcttc tacatttctc gttttcaact
    39421 ctttaatagg ctgggcaaag gtatatctct cccctaaagt cctaaaatca atcactgttt
    39481 ttctatgcat accttaagta tagcataaaa aggcagatac agacaaagct gatctacctt
    39541 tttacgattt tattcattct atgcttgtgg tgcagtaaat agatcaccag agaaaccgtc
    39601 tgtgttttta aggagagctt taaagacttt cgatttcaag acaactgtat ttcggtattt
    39661 gtctttaata ctactatcgt ttagttccgt taaatcttta gtaacagcct catcaaacat
    39721 agtcttcaac tgttcatagg ttgaaatttg agtgccatta atttcaattg gtgcaaaacc
    39781 ttttttagat tttgtaagaa cttccccata ccattgcttc ttccaatctt caaggtttga
    39841 aaattggtta ttagaaactt tcttaataat gaaatcatca cctaatagac cgttattttc
    39901 tttattggct gcaccttgat gtttgcttga aacatagtca atgaacccat gttcatatcc
    39961 atagtaaccc cacatacgga aggcgttatg tttgaaggat agagaaccag gggcaccttg
    40021 actcgtatta ccgccataaa taccagccat catattaaca ttgacatatg ccgtaccaaa
    40081 gtcctctggg tggaagacct tattacctgg atttccgtga atggtcatga aattattatt
    40141 taccaagtca tcgatggttt caaggttagt tgttttctca gcgtctatca aatcacgcac
    40201 tttgtcccat tggtgaggac cacttagttt atttctgtcc attggtcgac gtgtttcgcg
    40261 atcaattttc ttgaaccact tactattgtc acctttaacc ttaggaagaa cagcctcagc
    40321 ttctgcataa tcgagcatca tcatagcttc attgtagttc ttcatgtaat ggtcgatttg
    40381 gtcacgtgtt tgaagtttgg taggatctgg attataccat tggtttccat catttgaacg
    40441 ttcaaatgcc atatttaatc caagtgcgcc atattcacct tgatgtccat gttctgctgg
    40501 agtttgtaac ataccttgag caaatgcttc aagatctgta ccctgtcgat gtctatgacc
    40561 tcccagatat gccattcggt catttacgtg ggtagtctca tgtgtaaagg cagatgtgcc
    40621 atattcactt attaaatccg acataacaaa atatacagca tccataggct gtggatcagc
    40681 aaagatgtaa gcatacgctc catacccata cttataatca tgatacttat caatacgtcc
    40741 aaagaattca cgaataggtg cataatccgg tttatctttt cgatgaccaa atctatcagg
    40801 ccaaccaccg ggtgcaccat gattatccca tacaggagtt ggaactacat tttgactctt
    40861 aagaagttta tcgcggacat tatcagtggc aaggcgtgac caaaaatcta agtaattaac
    40921 ttgctcttta gcacgtttat tgatttcact cttgaacgat tcacgttttt cttctgtatc
    40981 tttaccatac ttttcaaacg aactaaatga gatagtatta tatgttgaaa tcataaataa
    41041 atgtgcatct ttcaagttca gaagcgataa aatcatgcgt ccatgttgtc cgttattaag
    41101 tccttcatat agacgatatt ttttattagc aaatgcaggg ttagttgacg gttgttcaac
    41161 tacataagca ttattttcaa tagctttctt aaaccaaaca tttatgtctg tttcatttgt
    41221 gaatagtttc atattgtagg tcaagaagtc atgtaagttt ccagaaagag tatttttcgc
    41281 caccacttca cggaaggact cctgtgtacg acttccctta atatagtcct cttttgaacc
    41341 aatttctatt aatcgatcta aaacacttac attcttacca taaaaatctg gtttgaatag
    41401 cattaattct ttaatattga agttatcaaa tttcacaccg taataacgat ttaggtatgt
    41461 taatcctaac aggacagcag ctttgttatc ttcaattttc ttaatcaagg cacgttttgc
    41521 agcttcatct gtattcagtt gatgatcttc attttcaact aattttgtca caaatgtatt
    41581 aagatgatct tttacttctt taaagctttc ttctaagtaa agattcttaa ttgcattaac
    41641 tctgttatcc ccagttcttc ctaatcgttg ataaattgga tcagattgta attcaattgg
    41701 acttaaaata ccttcaatag cactaattaa atctgcacga tctttaacaa ccatatttgg
    41761 tgtatagacg acttcaccaa gctctgcgac actgtattct tttacttgct taacttgaga
    41821 gtctttagga gaaatcgtaa agatatcttt tgtcttatct gcgtaatgaa ccataatatg
    41881 gtcagcatct gtaaggtctg taataaagtc attacctttc attgcagtca cagacaatac
    41941 ttctttattc attaatggac tatcagttgt aagcttgttc ccttgattca caatccattc
    42001 tttgttgtag aacggttgga gtttttcaac attacgataa acaagctcac gatttgcatt
    42061 ataatcttga attgttttgt attcttcatc acgtaatata cggcgattca acttatcaac
    42121 caaatgctca gttacttcaa atgtatcagc agtgataccc atatccttaa ttttcttatc
    42181 agcttctacc tgtgaaattt ctttaatttt agacgattgt ttatgggttt tatctccaga
    42241 actcacaccg ataactgttg cattattagg tttaatccaa ttgcttacaa attcatcatc
    42301 catatcatta gaaccaaaga aaatatcacc acgcttaaca ttcactaagc tgatattgtt
    42361 atcaatggtt ccccaaggcc agtctttacc aattaaaccg cctacatcac ctctagcaac
    42421 atcaatagta cctttggcta cagaatttct tacattagac caagcaccaa catctcttgg
    42481 attaaaatca tgagctaaat gtcctactat agcacctgca cgagtattta aacctttaag
    42541 ttcaacatct gcataagctt tatttaaatt gcctcgataa atctcagatg caatcccagc
    42601 caagttccac cctttatcac caacagattg aacttttccg ataaaagcaa cattagtcat
    42661 aatacctgca tcatctaatt tatccaaaat acccgcaaca ttgttattac caacgatagt
    42721 acccgttact ttaatatttt caacagttgc atttttcact gtttgagcta atgaagctaa
    42781 ttgatttgcc caaggcatat taatattaac gttaattaaa ttaatatttt tgatagaacc
    42841 cccttcaact ctatcaaata atggacgagc agtgttatga atggagaaat gcttaccatc
    42901 aacacttgtt aagtgacctg taaatttacc tgttacatat gatttacttg gtgtaggtac
    42961 atttgttgca tttaaatccg atcctaattt aaactcaccg ttaggatttt tctgcatagc
    43021 atctactaaa tctttgaagt tatagtaaac gttattttgc gatgctttgg gtttctctat
    43081 gtaataagtg tatgtttgac tcaaactatt atttgaatca cgttggatta aatcaggagc
    43141 tgaagcagta actttaaata aggatgtctc ctcctcagtt acttcctcta tattagtaat
    43201 ggcaagcctt gttactttat tatgattagt ggtaattttt agataatatg acgttagatt
    43261 ttctggaaca gtttctagaa ggctactatc tgtttcattt ccattctcat ccactttcat
    43321 caatcttgtt tctttaatat ctttaatctc aactttttta aggtctaact gaacttcttt
    43381 atcttctaat atttcagttt cttcaccgtt acctcgatca tagaccatgg ttgtggatag
    43441 tgtataacct tgataatatt tcaagttgtc taaagtagct tgtaagttat tttcttctac
    43501 cgttacttct ttaacaactg ttccagtatt atcttttaga gctactttaa tcgatttaat
    43561 ttctactcca gctggtttag tcaatgtata actagctgtc gcactcttct tcaagacatc
    43621 tttatctgtt ttagcccatt ccaaggttgg tttctctaac tcttttgtgc cattcttaat
    43681 aattttgtct tgaggttttt caatcactgt ttctgtgata gtaggagcgt cacttgtttt
    43741 ttctccgcga attgttttat aggtttcagt aatttgtttc ttatcgttta caccttcttg
    43801 agttacttgt cttgttccgc gttttaaagt gtcatcttct tgttcttttg tttcaaaagg
    43861 aatgatttct tctcttgtct caaccgttgt cccttcaatt ggttttgtac cacggattac
    43921 ctgttgattt acaggctcct tggtgacttc cgtactggtt gaaagaactt ggtcagtttt
    43981 aacccctcct attgttttat aggttgtttt tgtaacttga cttccttttt caccatttcg
    44041 taggacagtt tcttcgtccg tgtacttggt tgggtcctca gtcgtttcag ttttatagtc
    44101 aagttctgta gttgattctt caacgagtgt tccttcagtc accttatact caggtaattg
    44161 ttctggaaca agagcctcac ccacgtaatc ttcttcttgc gtccccttag ctgttttagt
    44221 ctcctctttt ggttctacag tttcttcagc cttggtatct ggagttgact cttcttgttt
    44281 cggtgtctct tccgctcccc tatcttcttt aggattgtct tcttgtgatg attcttttgg
    44341 tgtacttggt tgctcagctt gtgtaggttg cacgggttgt tctgggactt ttgcttgttc
    44401 tactggtggt tcaacttttg attcctcagc tggtttgtct gatggttgac tttctggctt
    44461 aactgctact ttttcctctg attttgactc aacttctcca cctacttctt caactggagc
    44521 tggttctgct gaatcttctt tcccctcttc tactttagga agggtgtcgt cagtaggttt
    44581 tacctccgat tttggttctt cctttggact ttcttctgtt ctaggtgctt cttcttttgg
    44641 agtttcctct gtctctacta cttggttttc tgtcctagct tgctcctgat ttgttgttga
    44701 ttgaggagtc tcaacttcga ctacagtcac ctctccaggt tttgctgagg tttcttctaa
    44761 aacagtgtcc aagccaagcg ttttgaggat gtcacctgat agataaccaa catagcgata
    44821 gccatccatt tcaacaacac cctctcgact agccagcgct aaggtcgcaa ctgggtctac
    44881 agcccctgca ctaggaagaa ctaccaatcc catagctcca actagaaaga cgctagcaat
    44941 tttctttctc ttgtagatta aaagcaagct cccaacagtc agcaaaccaa aagctgtcaa
    45001 aacagatgct tctgtccctg tttgaggcaa ctgatctttt tgatacacca aaccatatac
    45061 aacttcattc ctgtcaggct ttcctgtctg aattaaatct ttagcttctt gtgaaataat
    45121 ctctttattt acatagtgat aggtagctgc gtccactaca gaaggagcca tcaaaaggct
    45181 tccaagaaat acagagccta caactccctt aatcttacga attgaaaaac ggtctttttt
    45241 aaacactttc atctccttta ttcattctca agactttcta atagcatctt gcggatagtg
    45301 cgcacgcgca cctccgatta attttggacg actagccagt gccgttacat gggcatgacc
    45361 aatctctctc aaaatagggc gaatcggaac ctgaacatgc ttgacatgca tgccaattgc
    45421 agtgtctccg atatccaatc cagcatgagc cttgataaat tcaacctcaa ctggatcctg
    45481 cataaactta aaggctgcca actgccccga acctcctgca tgaagagtag gatggacact
    45541 gacaatttcc agatcaaact gctctgccac ctgacgttca acaacgagag cccgattgac
    45601 atgctcacaa ccttgaactg ctaaatggat acctctacta cctagaatac ccaagatagt
    45661 ctccactatc agctcaccaa tctcttgact ggattctttc ccaatctgac cacctagcac
    45721 ctcactagaa gatagaccta aaacaaaaag gaccccctgc ttcaaattgg tcttttctaa
    45781 aacatcttcc actacctgac gtgtttctct ttgaatctgt gtctcgttca tctctgttac
    45841 ctctgttgtc actcttctat cataccgttt tttcttgttt ttagcaagat agacaaccta
    45901 taaagtctgc ccaattacgc ataaaactcc cagaattgac tgggagttag ctagtttcta
    45961 ttctatttat atatatttca actttcgttc ctttttgggg tctagaatca atcttcatct
    46021 ggtaattggc tccaaaatga agtttgagcc gttgatcgac attttgaaga ccaactcccc
    46081 cacgtttgag ttgactttga ctactatcac cagcatcttg gaagccaacg ccatcatcct
    46141 caatacggat gaccaatccc gaatcctgtt tctggacaga aagtttaata tggccctgac
    46201 cttccttttc cttaatgcca tggtaaagag cattttctac aaggggttgt aggaccagct
    46261 tgggtaagac taaattatca aaggcaacat tttcattaat ttcgtattcc agcttatctc
    46321 catagcgttg tttctggata aagagatact ggcggacatg attgatttcg tcagagagac
    46381 aaatcaagtc cttgccttga ttgagcgcca agcggaaata ggttgccaag gacttggtca
    46441 cctgcaccac tcgctgacta tcatgaaatt cagccatcca gatgatggtg tccaaagtgt
    46501 tatagaggaa atgtggatta atctggctcg aaagggcttg aagttggtac tgacgggtcg
    46561 tttcttcctg gctacgaata gctaccatca actgatcaat ctgatccaac atagcattaa
    46621 attggcgagt tacttctctc agttcatagg caccaacttc cttggcacga agattttgag
    46681 caccagaagc aatttccaac atggtttctc tcaaatcctt caaaggagca atccagcgtt
    46741 taagactgaa ccacactaag cagagacaaa caagaagaga tgtgacactg gccccaagca
    46801 aggtccacaa gagctgactc cgaacctggt ctaacttttc caatgatgac acgccaagta
    46861 ccgtccaatc agttcctgca atcttctctt gactgacgta ggatttgtga ccaggagtat
    46921 aaccctgacc tgtctcgatg tagggtttca tagcctccat tttgctagac gaactataaa
    46981 ctgtgtgttg aggatggtag acaaattcat ggttttcatt gataatgaag gcaaagccct
    47041 gctgccccaa ctggagttga ttgagatagg cttccagagt ttcataagaa atatccaaac
    47101 gaagcacacc aagattggct ccctttgcat caacaagttc ttgagtgaca gaaatgaccc
    47161 actgactatc tgatttacga gctggagtca aaacaggcat agctccctga tgaatagcct
    47221 tttggtacca atcctcagcc atcatatcag aggaagtttt catctgcaca ctgtcatctg
    47281 tagaaatgac ctgaccagat ttggtcacca gcacaacagt tttcaagtcc ttatctgact
    47341 tcaagatggt caaaaacaaa tctcggattc cctcgacctt gtcttgactg ggattctcag
    47401 cataggccag aacatccgtc tgctgggtca aaccagtcga ggtggtttct agttttttga
    47461 tataagactg aataaagtgg ctagtctggc tgatggtcgt ttggctgttg ccctcaatgg
    47521 tggcctcaat ggctgaagaa cttgattgat agtagaaagt tccaaccaga gctaggagaa
    47581 tgagaaagac cagaaagatg gaaataacca ttctaactaa aagagaagaa cgcttcatcg
    47641 gtcttctccc ttcttaaact gacgaggtgt cacacctgca atctgcttaa aacgttgggt
    47701 aaaatagttc atatcttcaa aaccaacctt ctctgcgatc tcataaatct tcagatctgt
    47761 agttaaaagc aagagcttgg cttgtttaac acgttctctc accagataat cctgaaaagg
    47821 caagcccaac tctttcttaa tcaaggaact cagataggtc ggactaaaac ctaagtcact
    47881 ggctaaagac tttaaactaa attggctatc agccagatga gactggattt tctgggccat
    47941 gtttccttca aacctattag tcaataaatc ttgtaactgc tcttctttct cttccttgtc
    48001 tagtttttgt ttgattttcc ccaacatttc ctcaatatcc tgacgagaaa agggtttgag
    48061 caggtagtcg tccacaccta gtttgacagc agacaaggca taatcaaaat catcgtaacc
    48121 tgttaaaaag accaaatgaa cctgaggata ggtttctcgt accagactgg ccaactggat
    48181 gccatttaga tgaggcatgt tgatatcggt taaaatgata tctggcacct gcttttggat
    48241 caattcccaa gcctgccttc cattttcagc ctgaccgatg atttccatat cgtaggctgc
    48301 tacattgacc agtttagtca aaccttgtct taccagatat tcatcttcta cgattaagat
    48361 tgtgtaggtc atgctctgct cctttaccac ttactagtat cagtatagca aaattctcct
    48421 ctaactgctt aggaaagacc tcttatactc aataaaaatc aaaaagtaaa ctaggaagat
    48481 agccacaggt tt
//
LOCUS       DAVBCG010000003        43592 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBCG010000003 DAVBCG010000000
VERSION     DAVBCG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.003"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(38..1312)
                     /gene="psr"
                     /locus_tag="VQ495_000123"
     CDS             complement(38..1312)
                     /gene="psr"
                     /locus_tag="VQ495_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2530142.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(1309..2157)
                     /gene="pheA"
                     /locus_tag="VQ495_000124"
     CDS             complement(1309..2157)
                     /gene="pheA"
                     /locus_tag="VQ495_000124"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2530143.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(2154..2630)
                     /locus_tag="VQ495_000125"
     CDS             complement(2154..2630)
                     /locus_tag="VQ495_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2530144.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQVWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(2623..3906)
                     /gene="aroA"
                     /locus_tag="VQ495_000126"
     CDS             complement(2623..3906)
                     /gene="aroA"
                     /locus_tag="VQ495_000126"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2530145.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(3998..4336)
                     /locus_tag="VQ495_000127"
     CDS             complement(3998..4336)
                     /locus_tag="VQ495_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2530146.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(4347..5450)
                     /locus_tag="VQ495_000128"
     CDS             complement(4347..5450)
                     /locus_tag="VQ495_000128"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2530147.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(5460..6626)
                     /gene="aroC"
                     /locus_tag="VQ495_000129"
     CDS             complement(5460..6626)
                     /gene="aroC"
                     /locus_tag="VQ495_000129"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2530148.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(6636..7703)
                     /locus_tag="VQ495_000130"
     CDS             complement(6636..7703)
                     /locus_tag="VQ495_000130"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2530149.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVENILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(7722..8576)
                     /locus_tag="VQ495_000131"
     CDS             complement(7722..8576)
                     /locus_tag="VQ495_000131"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2530150.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKKMPTEEIW
                     QSLTEKYQ"
     gene            complement(8566..9243)
                     /locus_tag="VQ495_000132"
     CDS             complement(8566..9243)
                     /locus_tag="VQ495_000132"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2530151.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(9240..10403)
                     /locus_tag="VQ495_000133"
     CDS             complement(9240..10403)
                     /locus_tag="VQ495_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2530152.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDERRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(11088..12722)
                     /locus_tag="VQ495_000134"
                     /pseudo
     CDS             complement(11088..12722)
                     /locus_tag="VQ495_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12703..13452)
                     /locus_tag="VQ495_000135"
     CDS             complement(12703..13452)
                     /locus_tag="VQ495_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530153.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            13631..15085
                     /locus_tag="VQ495_000136"
     CDS             13631..15085
                     /locus_tag="VQ495_000136"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2530154.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(15175..17793)
                     /gene="alaS"
                     /locus_tag="VQ495_000137"
     CDS             complement(15175..17793)
                     /gene="alaS"
                     /locus_tag="VQ495_000137"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2530155.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(17815..18300)
                     /locus_tag="VQ495_000138"
     CDS             complement(17815..18300)
                     /locus_tag="VQ495_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2530156.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLLDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(18575..18814)
                     /locus_tag="VQ495_000139"
     CDS             complement(18575..18814)
                     /locus_tag="VQ495_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530157.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILEK"
     gene            complement(19067..20137)
                     /locus_tag="VQ495_000140"
     CDS             complement(19067..20137)
                     /locus_tag="VQ495_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2530158.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNIPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(20134..20907)
                     /locus_tag="VQ495_000141"
     CDS             complement(20134..20907)
                     /locus_tag="VQ495_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2530159.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(20904..21710)
                     /locus_tag="VQ495_000142"
     CDS             complement(20904..21710)
                     /locus_tag="VQ495_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2530160.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(21691..22848)
                     /locus_tag="VQ495_000143"
     CDS             complement(21691..22848)
                     /locus_tag="VQ495_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530161.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(22907..23857)
                     /gene="murB"
                     /locus_tag="VQ495_000144"
     CDS             complement(22907..23857)
                     /gene="murB"
                     /locus_tag="VQ495_000144"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2530162.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(23969..24721)
                     /locus_tag="VQ495_000145"
     CDS             complement(23969..24721)
                     /locus_tag="VQ495_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2530163.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWFEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(24731..25462)
                     /gene="budA"
                     /locus_tag="VQ495_000146"
     CDS             complement(24731..25462)
                     /gene="budA"
                     /locus_tag="VQ495_000146"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2530164.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            25604..26875
                     /locus_tag="VQ495_000147"
     CDS             25604..26875
                     /locus_tag="VQ495_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2530165.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(26895..27710)
                     /locus_tag="VQ495_000148"
     CDS             complement(26895..27710)
                     /locus_tag="VQ495_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2530166.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(27867..28520)
                     /gene="phoU"
                     /locus_tag="VQ495_000149"
     CDS             complement(27867..28520)
                     /gene="phoU"
                     /locus_tag="VQ495_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2530167.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(28532..29290)
                     /gene="pstB"
                     /locus_tag="VQ495_000150"
     CDS             complement(28532..29290)
                     /gene="pstB"
                     /locus_tag="VQ495_000150"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2530168.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(29303..30106)
                     /gene="pstB"
                     /locus_tag="VQ495_000151"
     CDS             complement(29303..30106)
                     /gene="pstB"
                     /locus_tag="VQ495_000151"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2530169.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(30117..31001)
                     /gene="pstA"
                     /locus_tag="VQ495_000152"
     CDS             complement(30117..31001)
                     /gene="pstA"
                     /locus_tag="VQ495_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2530170.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(30991..31908)
                     /gene="pstC"
                     /locus_tag="VQ495_000153"
     CDS             complement(30991..31908)
                     /gene="pstC"
                     /locus_tag="VQ495_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2530171.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(31908..32786)
                     /gene="pstS"
                     /locus_tag="VQ495_000154"
     CDS             complement(31908..32786)
                     /gene="pstS"
                     /locus_tag="VQ495_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2530172.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(33360..34664)
                     /locus_tag="VQ495_000155"
     CDS             complement(33360..34664)
                     /locus_tag="VQ495_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2530173.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(34665..35438)
                     /locus_tag="VQ495_000156"
     CDS             complement(34665..35438)
                     /locus_tag="VQ495_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2530174.1"
                     /translation="MKSKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(35428..35706)
                     /locus_tag="VQ495_000157"
     CDS             complement(35428..35706)
                     /locus_tag="VQ495_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2530175.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGKGKISLEK"
     gene            complement(35706..36107)
                     /locus_tag="VQ495_000158"
     CDS             complement(35706..36107)
                     /locus_tag="VQ495_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2530176.1"
                     /translation="MITLFLSPSCTSCRKAKTWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(36317..36934)
                     /locus_tag="VQ495_000159"
     CDS             complement(36317..36934)
                     /locus_tag="VQ495_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2530177.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(36924..37697)
                     /locus_tag="VQ495_000160"
     CDS             complement(36924..37697)
                     /locus_tag="VQ495_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2530178.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(37708..38445)
                     /locus_tag="VQ495_000161"
     CDS             complement(37708..38445)
                     /locus_tag="VQ495_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2530179.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(38450..39580)
                     /locus_tag="VQ495_000162"
     CDS             complement(38450..39580)
                     /locus_tag="VQ495_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2530180.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(39664..40053)
                     /locus_tag="VQ495_000163"
     CDS             complement(39664..40053)
                     /locus_tag="VQ495_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2530181.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(40069..40452)
                     /locus_tag="VQ495_000164"
     CDS             complement(40069..40452)
                     /locus_tag="VQ495_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2530182.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(40453..41241)
                     /locus_tag="VQ495_000165"
     CDS             complement(40453..41241)
                     /locus_tag="VQ495_000165"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2530183.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(41234..42169)
                     /locus_tag="VQ495_000166"
     CDS             complement(41234..42169)
                     /locus_tag="VQ495_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2530184.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(42299..42475)
                     /locus_tag="VQ495_000167"
     CDS             complement(42299..42475)
                     /locus_tag="VQ495_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2530185.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(42615..43322)
                     /locus_tag="VQ495_000168"
     CDS             complement(42615..43322)
                     /locus_tag="VQ495_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2530186.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            43473..>43592
                     /locus_tag="VQ495_000169"
     CDS             43473..>43592
                     /locus_tag="VQ495_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET2530187.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQD"
BASE COUNT        12892 a        10059 c         8330 g        12311 t
ORIGIN      
        1 ttcctagttt gcactttaat tttcattgag tattatctta tctcaagccc atttgagcga
       61 gcttggtttg atatttgttt tgatcaacca gcaagcccaa gcctccataa acatcatagg
      121 catctaccca gtcacccagt tctggaatcg tcaatttttc aataccattt tttgctccat
      181 ccaaaacaga taaaccgttt gttaggagga aagtataggg tacgttggtt gaggtcatag
      241 caaaaacctt tccaagagct tcagaaccag tgaaaagttt agtgggatct ttaatttgct
      301 ctaaaattgc tgttaaaact tgttgctgtc tttttgtacg gccgtaatcc gcctcatcat
      361 catcacggaa acgagcataa ttgagcaggg tcgagccatt catctgctgt tttccgactt
      421 taatggtttg ggttggagac tcagtctcgg tagcgtataa atcatctccg actgtagctt
      481 ctgttagggg acgcccattc aatgttgaaa attgagcatc aatcgtcacc ccatcaggga
      541 aaagcgtgtc aatcgctgtg gcaaaggcct ggaaatcaac caaggcgtag tacttaatgt
      601 ccaagtcaaa attatctttc aagacttggc gaaccatttc tgcccctttt tgcccctctt
      661 gttctcctaa ctcgtaggct acgtttaact tgttatctgt ctgttttcta ccattaatca
      721 cttgactata accatctata tagaccaaat tatcacgcat gaaactgact agcttcattt
      781 tcttatctga gcccccgaca tttaatacca taatagagtc agttcgtgtc tcaacactgt
      841 tctggccgat tcgaccatca gtacccatga ttaaaatatt aactccatct ctagtgtcct
      901 gaccattaaa gacttctact tgagctgccc gggcatcagc agttttcttt gcgctagcat
      961 cttggtaacc acgcaaaaac atgaatacca tggccaaagc cacacagacc aaaagtgaaa
     1021 aaatcaccat aaaaattcgt ttaagacgga gcttccgtct tttctttttt ggagggaaag
     1081 agagtgcttg tgatttggat tgtgagcgac tccggttcgc atagcttggt aagtcaacct
     1141 gctcttctct ttcttgttcc aagctagagc tactatttcc cctagcaaga gttagctttt
     1201 cttgcaaata ggcaaactca tttttttctc tctcattgag atagtgaata ttttttagca
     1261 aataatcata acgcaactgc tcatgatgac ttaagggatt ttctttactc atcttctctc
     1321 ctttccatgg tctgatattg gataaatagg ataggcaccc agaattttat actggattcc
     1381 aatcgcttct aattcttttt gggcaaagtg gaccaagtcc ttatcgatat aatccacatc
     1441 gataatgaaa aagtattcac ccagtgctgt cttgagtgga cgactttcaa tttttgtcaa
     1501 gtcaattcct cgccaagcaa aggtcgacag ggccttataa agtgcacctg gaaggttgtc
     1561 aggtaatgtc aaggccaaac tcatcttttc agtttgtgct tgcaagggaa tactaggctt
     1621 ttcagctcct agaacccaga aacgtgtgaa attggcttcc atttcctgaa tatcctcggc
     1681 aatcagttcc aatccatatt cttcagcaga acttctaggt gcaactgctg caaagggctg
     1741 gtctggatgt tcggaaataa aacgggccgc ataagctgta ctagctgtta cctcgatttg
     1801 agcctctgga tattgttcat cgatgaattt ctttccttga gccaaggcct gtggatgtga
     1861 aaaaatcttt tcaatcttag tatggcctgg aaccaccatc aactgctgat gaataggctg
     1921 aacgatttct gctactgctt ggatgtgagc ctgatgaaaa agatagtcca aggtttcatg
     1981 aacactaccc tcaatagaat tttcaactgg caccacagaa tagtccacta atccttgctc
     2041 ataagccttg atgacatctg taatgttggc aaaagcctgc aattcctcat gaggaaaagc
     2101 tgtctgcaca acgtggtgtg aaaatgatcc cttgggacct agataagcta ttttcatctt
     2161 agttcctcta taatttcctc tgggcttagc ttggtcacat ccaaaacccg actagccact
     2221 tcctcatacc aaacctgtct ttcttgaaaa atagctacta gttcttcctt gctattattt
     2281 agaaaaagcg gtcgctgatt gtccttatca gctgcgatac gtaggtagag ggtttcaaaa
     2341 tctgctttca ggtagatgtt atctgtatta gtcttgagta agtcacgatt tctctgagaa
     2401 ataaccactc ctcctccagt tgacacgact tggtctgttt gtagtaaatc agctaggact
     2461 tctgattcta catgacgaaa ggctgcttct cccttttcag cgaaaaaatt cgcaatggac
     2521 atacctaggc gcttctcaat cagagcatcc atatcaaggt aattagggtc caagcctctt
     2581 gcaatagtcg attttccagc ccccataaac cctaataaca ccttagccat gaatcaagct
     2641 ctccaaatca tcaaagaaac taggatagct ggtattgatg gcttctgcac ggtcaagctc
     2701 cacctctcca tctgcaacca agagggctgc gatagctgtc atcatgccga tacggtggtc
     2761 accaaacgta ttgactctag caccgtgaag agctgatttc cctttgataa tcatcccatc
     2821 tgccgtagga gtaatatctg ctcccatact atttaaggcg tctgccacaa cctgaatacg
     2881 gtctgtttcc ttgaccttga gctcctcagc atccttgata actgttacac cttgggcttg
     2941 agtcgcaagc agggcaataa tgggcaattc atcaatcaat cgtggaatca aagcgccacc
     3001 aatctctgtt cctttcaagt cagaagactc aacaatcaag gtagcagatt tagcgactgg
     3061 atcgatttca gttatttcca attttccacc catggcacga atgacatcaa taataccggt
     3121 gcgagtttcg ttgatcccca cattctgcag cactagacga gaatttggag caatcaaacc
     3181 tgcgactaac caaaaggctg cactggaaat atctcctggt acgaccacct tctgtcctgt
     3241 caatttttgt ggcccctgga ctgtgatttt cttaccatcc acacttaaat gaccaccaaa
     3301 ttgttgcaac atatcttcag tatgattacg ggtgtactct ttttcgataa taactgactc
     3361 ccccttagct tgtaaggctg caaacatcaa ggctgacttg acttgggcag aggcaattgg
     3421 caactcataa tgaataggtc ttaggttttt cgtccctttt aagcgaaggg gaggcaagtc
     3481 tcgttcagtt tgccctgaaa tgctgacgcc catttttttc agtggaaggg tcacacggtc
     3541 cataggacgt ttggaaagac tatcatctcc aaacatctct acttcgaaat ctgcaccagc
     3601 aaggacacct gaaatcaggc gaatcgaggt gccagaattt cccatattaa gggcattttg
     3661 tggcgctttt aagccagcca tgcctacacc ttgaatggta ataaccccat ctttatcctc
     3721 aatttcaaca ccaaggtcac gaaaaacctg catggtcgaa agaacgtctt cacctcgcag
     3781 aatatcataa accttggtct caccctcagc caaacttcca aagataatgg aacggtggct
     3841 gatagacttg tcacctggga cgcggatact accatgtaaa tggcgaatgt ttgtttttag
     3901 tttcatactg gacctcatac ttgcaatact tttacctatt ttatcataaa aagtcagaaa
     3961 ttccttaaaa attcctgact ttaggatcgt tcttttctta tttcagcaat tctgaaactg
     4021 gttcaaaaac aattttttca atgtcagaaa ggtaaatggc caattgttgt tgcttggtaa
     4081 agaattctga caagaggcta tttccttgaa tctgtttacc aaagccttcc atcttagctt
     4141 ggaaggacgc atctggcatt tgacctgtct gtgctagttt ttgaatttcc tcttgaaagg
     4201 caagatattc tgtaaagatt ttgcttgcct cagcatctgc tgcaatcgca tctttagctg
     4261 ctttaacagc cttgtattct ggtaatccgc gtagaccgcg actgagttcg tttgcactat
     4321 cgtaaatatt tgacatgttc ttctccttat ttgatgacga ctgtatagtc ggtattttct
     4381 gttatgagat gctcagctct ttccaagtct tgagcatttt taaatgaaat ttgtaggatt
     4441 ccgtgaatat cctcacgatt ttcctcgttg atgtggatgt taaccaagga agttccacgt
     4501 agcagttcca aaatccgcag gatgacatct tcttcatcag gaacgtcaac atagaggtcg
     4561 taagagctat ccacaccacc acgcttatgg atttccatgg tctggcgttg ttcacgcgct
     4621 tggttaaaaa agttccaaat ttgctcttca tctcccttac taatggcctg accaatcact
     4681 tccaaacgtt ccttgaaatc ctcaattcta tccagaatgg tctcgctatt ggacaagaga
     4741 atggaggtcc acattcctgg ctcgctttcc gcaattcggg tcatatctcg aaaaccacct
     4801 gccgcaaagc gccttgccat ctcatgctct tgagcataga ccgcagtctg ctccatgaga
     4861 ctagaagcca aaatatgagg aaaatggcta atctgagaag tgacacgatc atgctccttg
     4921 gcatcaatct cgataaaacg agcatgaaga cctgaaagca gatccttcat ttccttaagc
     4981 gtgtcctgac ttgtcaggct tgaaggtgta aagatataat aggcattttc aaaaagattg
     5041 acatctgccg aagcagcccc tgtcttgtga ctaccagcca tgggatgggc cccgacaaag
     5101 cgaacagact tgccagccaa atactgctcc gccgcatcca caatggttga cttggtcgaa
     5161 ccagcatctg aaataataac gccttctcgc aaatccaaat tggccaactc cttaatgaaa
     5221 gcaatagttt gtttgattgg caagctgagg ataatgacat ctgccaaagg agcaaaacta
     5281 gcaaaatcat ccgttgcacg gtcaatcata ccttctttca aggcgatatc tctcgaagct
     5341 tgactacgat tataacctaa aatttcataa tctggatgat cgcgtttgat accaagtgcc
     5401 atagaggctc caatcaaccc aagacctgcg atatagattg tttttgccat aggaactcct
     5461 taatagttct ttgtatagtc tcggtgtttg gctaccgctt cttttagttc ctcaagatta
     5521 tctgatgaga atttttcgag gatttcttgc gccagaaccg ttgctacaac tgcttccatg
     5581 accattcctg cagctggaag agcagtcgga tcacttctct ccacggttgc cttgtaaggt
     5641 tcgtgggttt cgatatccac actcataaga ggtttataaa gagtaggaat gggtttcatg
     5701 accccacgaa caacgatggg ttgcccatta gtcataccac cttcaaaacc acctagatta
     5761 ttggtacggc gagtataacc gtcttcttta gaccagagaa tttcatccat aacttggctg
     5821 cctttacgat aaccagcctc aaagccaaga ccaaattcca cccctttaaa ggcattgata
     5881 gagacaacag cttgagccaa tcttgcatcc aattttctat cccattggac ataggaacca
     5941 agaccaactg gaacgcctcc gacgactgtc tccacaaccc caccgatggt atcaccatca
     6001 cgtttgattt ggtcaatata gtccttgatt tcctgttctc gttcttggtt gacaatagaa
     6061 acttcagact gggcagctct ttgcttaatt tcagcgactg tcagattttc aggaacatct
     6121 atttccttac caccaaagac cacgacatgg ttggcaatct ccatatccag ctcagccaag
     6181 aggcgtttgg ctactgcacc aactgccacc cgcatggtgg tttcacgagc tgatgaacgc
     6241 tccaaagaat ttcgcaaatc atcaaaacgg tacttaatcc ccccaaccaa atcggcatga
     6301 cctgggcgag gatgagtaat tttccgcttg cttttaaggc ggtcttcaat gtcctccgca
     6361 gacatgatgt ccagccattt ctggtggtcc ttattgatga catccatagt aataggcgcc
     6421 cctgtcgtct tcccgtggcg aacacccgaa gtaaagacaa cctggtcact ctcaatcttc
     6481 atacgaccac cacgaccgta gccaccctga cggcgtctaa gatcctcatt gatatcctca
     6541 gctgtcaatg gaagtccagc tggaattccc tcaataatag ctgttagacg ggggccgtgt
     6601 gattctcctg cagttaaata tctcatacac tctccttatt ttaccaagta gtctttcatc
     6661 tcttccagag aaactgggtg aatggtcgct gaaccaagct ctggcaccaa gaccaatttc
     6721 aaggtgttac cacgcgcttt cttgtcatga gtaagagcct gataaagctt gtcaactttc
     6781 caattttcat agtcaacagg caagccgaat ttctgacaca tctctgtgat agattgggta
     6841 atgccagctg gcatgaggcc tttttcctca gcaaccttgg aaatctgtac cattcccatg
     6901 gcaacagcct ctccatgcat gaccttgcca taaccggcag tcgcttcgat ggcatggcca
     6961 atagtgtggc caaaattgag gtaaagacga ataccattgt ccaactcatc ttcaaccacc
     7021 atcttgcgct tcacctgaca agaatgttca atcaaggtct ctgcatgttc caaaatattc
     7081 tcaacagaac cattcagtcc cgtcaagaga gcccacagtt ctggatcctc aatcaagcca
     7141 tacttgataa cttcacccat cccttcaatc aactctcttt ttccgagggt ttcaagaaca
     7201 agtggatcaa tcagaacccc atctggttgg gcaaaggtcc ccaccatatt tttagcaaat
     7261 ggtgtgttaa cgcctgtctt tccaccgata gaagaatcaa cctgagctgt caaactagtc
     7321 ggaatctgaa caaagtgaat accccgcata taggtagagg ctacaaagcc agccaggtcc
     7381 ccaacaacgc caccaccaag agcaacgatt ccatcgctac gagtcagacc ttgcttgact
     7441 agaaattcat agactttctg aacagtagtt aaattctttc tttcttcacc ttctaagaaa
     7501 tcaaaaacag ctacctgaaa accagcatct tctaggctga gcttgacctt ctctgcatag
     7561 agagaggcta catggttatc tgtcacaatg actacctttt gcggttgcca gagttctcgc
     7621 aaccactgac cagcctgggc catacaacct ttttcaatct gaatatcata aggatgatga
     7681 ggaatatcga ttctgatttt cataggagag tctccctttc tttattgata tttttctgtt
     7741 aaagactgcc aaatctcttc tgtcggcatt ttcttgcctg tccacagttg aaaagcttct
     7801 gcagcttgat agagcaacat tcccagacca ttgactgctg gattgccctg acttctagcc
     7861 catttcaaaa acggtgtttc aaagggttgg tatatgatat ctgcaactaa gagagtttct
     7921 ggtaagacta tgttttcagg aacaggagag gattggccat ccatgcccac actggtggca
     7981 ttaactagca aatccgactc ggcaatcctt gcttgcagtt cagaaacata ttctaaagca
     8041 cacaaatcca ctttaaagcc tgtctgctcc tgtaacttgt ctaggtaagg tcttgttttt
     8101 tccatagaaa cggaacgaac aaagaccgaa atctgactga cgccatccaa aatagcctgt
     8161 gccaagattg atttagccgc accacctgca cccagcaggg tcatcttttt acctgaaatt
     8221 gtaaaagaag gcaagcactt aaaaaatccc ttgccatctg tattatatcc aattaaattg
     8281 ccattctcat tgacaaccgt attaaccgca ccaatcaagc gcgcttcatc gctcagctca
     8341 tccaaataag gaatcacctg ctccttatag ggcatggaca gattgatgcc aaacatctgg
     8401 tagcgacgaa tattggccac tgtttctacc aagtcactcg cttcaatctc ccaagccaca
     8461 taagcaccgt tggtagctgt cgcctcaaag gctctattgt ggatgaaggg agaaatagaa
     8521 tgcttaatag gattggcaac aactgcagct aaacgtgtat agccatcaag cttcatccaa
     8581 aatctccctg atttttttca tgctagctag agaaatctgc ccaggggcac taacctcatc
     8641 cagactggca aaagaccaac tcgaaccagt cacatccgca gtgatacgag agaccttgcc
     8701 caccttaccc atagaaatgg tcacatattc ctgttcagga ttgagcgttt taaagcctcg
     8761 tgtatagttc atcaagtcta agacatcctg ctccgtgtga gccatcaccg caaccttaac
     8821 aagttttgga tttaggatcg tcaactctga caagatttcc atcatgttct caggtgtttc
     8881 ttggaaatta tggtaactca aaacaagatt tgggaagtcc agcatttcct caaaaacatc
     8941 cttgtagcta tagtactcaa aatcaatata gtctggttga tagagttgcg caacttcctt
     9001 gattagatgg atatactctt ctggagaaag gtcgatttct ccaccttcgg agcgagttcg
     9061 tagcgtgaaa accaactcac ggcctgcgaa tttttcaaaa atggctggag ctacctgcaa
     9121 aatcgcttct ttaggcagat agtcggcacg ccattcaatg atgtcggcat ccaggtacct
     9181 cgtggcatcc agagcctgag cctcctctaa acttcttggc attactgaaa cgattaattt
     9241 catttactaa ccttcatact aatcaccttg aggtaattac tactttcatc ttttttatta
     9301 taggcaaaat ctgctggaag accatatttg tttaaaatct ggtaacttct tcctgcaaag
     9361 cctttatcaa tttgttctgt aaatttctga cgggaaacat tggcagcatt ggtactggca
     9421 atgataatcc ctcccggatt taaaatctca agactctggg aaatcaactt gtgataatcc
     9481 ttggccacag agaaagtttg ttttttattc cgagcaaagc taggcggatc taggacaatc
     9541 acatcgtagg tcaagttttt gcgtttggca tatttgaaat actcaaagac atccatgact
     9601 ataaaacgac gctcgtctgt gctgagccca tttgcctgaa aatgcgcttg agacaattct
     9661 cgtgaacgtt tggctagatc aacagaagtt gtctggctag ctcctcccat ggccgcagct
     9721 actgaaaaag ccgctgtgta ggaaaacata ttgagcaagg atttacccat agccaagccg
     9781 tcaactaaac taccgcgaac ctcatgctgg tctaggaaaa ttcctgtcat caagccatca
     9841 ttcataaaga cttgatacag gacaccattt tctaaaacag tgaaaaagtc aggtgcttct
     9901 tgaccataaa catgggcaga ttcatagtcc aaacccttaa agcggatttt ctcataagcc
     9961 cctaaaacct cagggaaaac ctgtctaaag gcttctgata tagtctgacg aatctgataa
    10021 acataagagt tataccaaga aaagacggcg tagtcgccat aaaggtccac tgtcagaccc
    10081 ccaaagccat ctccctcttg attaaagaga cgaaaggcag ttgtcaaatc atcttgatag
    10141 taggcgtttc tcttttcttt ggcttttcta aacaacgttt caaagaaagc ttgattgaag
    10201 gccaccttgt ctttgctgac aaaccagccc aagcccttgt tttgctgaga aaggtaggca
    10261 gtcccaagaa agtttccttc ctgaccctgc acctctactt cctgatcctt aagattgaca
    10321 ttctcaagat cactggcttc tagtaaaact agccccttag caagcttctt ttcaaccctt
    10381 ttgctgactc ttattctatt cataactacc attatatcaa acttttagac aattctcaaa
    10441 aaagaaacta cccttgcttt tttactcttc ttttaaaaaa tggtatacta gacttcctgc
    10501 aaaactagga agtaaatgtg taagaatcac aataaaaaat gctcttccgt cttggaggag
    10561 catttctttt tatcaacgaa aatcaaataa caaactatga aactagcctc aggttaactg
    10621 tgagattata ggtagagagg ttgaatcagc aatatgtgtc tgtcaaattt agtgacaaag
    10681 gtagtagaag aaagatgaag aaataaatct gcttcagtag atatctggaa aatttgattt
    10741 tatagaaaag ccttttgtta caaactcaat atactatcaa taaataatat tatagaagca
    10801 acaataatta aaatttcacc tatttgcatc attctatttc gaactctaaa tatatgttct
    10861 atcaaaaata cttggatcac acacattata ggaattaacg tttttgaaat tgaaaaatat
    10921 cccaataaat aaactataaa caacaataat agaactatat tatatttctt attcaaaaca
    10981 ttcctcccta tatttttgat taccaatctt aatcatttac aactacattc taacaaacta
    11041 taaaagcgtt tgtcgaattg aatttatcaa gcaagcaacc aactatctca tcttttttct
    11101 atttctgcca atatgcgtga caggtaataa tgatagccaa aaatagcaag agcaagcaag
    11161 acgataagag ctcctactcc caagttgatg gcaaggatag gggagagaga ctgaaccaag
    11221 aatatgctcc caattacaag ggccatcagg attgcactat aaataaacaa taaaactatg
    11281 gcgactatgc catttgaacg attcaccagg tccgtaatgc tactccaatt ggttgacaga
    11341 tttttaacgt ccttaaagta atggtggcaa gaaaggatga cactggcaat gatccagact
    11401 acaagaaggt aaatcatcga aaggatggac aagcctagat atagagaaag accgagcaaa
    11461 gtcagaactg gtaaaaagga ctgaacagca aagataatcc aaaatttcac tttcacataa
    11521 cgagcaaagt caaagggtaa actcttcaga aaatcaacat tttccctctc caaggacaag
    11581 gcaattgaat gcaggctggt gatattgtta ttgacaactg ctataaacac tcctacaaaa
    11641 aacaagggta accagtatgg aggatgaatg tctggaacta tctgagaatc tcggattttg
    11701 gaaatcagac tgatcatcat gagataagga aggaaagcac ttgtaaaaag cactgtaatc
    11761 acaccagtcc cctatcccaa gagggtgagg tggtagcgta aaaccatacg gaaaaagccc
    11821 tttttagtag ttgaaatcct ctccttgctg cgacgttctt ttttgacctt ctcctcactg
    11881 ttaagcagga tcacgtcata aaaacgagga aggaccttct ttttggtcag ataaagcagg
    11941 aagagagtta gtcctatcca agcgagcaga cccactaagg cttctgtcga aaaaggctcc
    12001 actgctattt tgtaaaagat atgaagagga taaaggagaa atggaatgtc tctaactttg
    12061 tcaacaatac ttccaaaagt cgactgaaga aagaagataa atattaaagg tatgagaact
    12121 cctatcccaa tcatcacatt cgaaaaaata gactgatact ttctgaagac cctagtctga
    12181 gccaagaaat gtactgccac taccgtcact aaagtaacag agacaaataa taaggtcaag
    12241 gacagtagca tcaaaggcaa acccagccaa agagaaggag ctagactaat atagagggct
    12301 agaaaataag ctaggattgg tacaattcca gttagagctg gcaagaggac agacagacct
    12361 ttggccatta taatctctga ttctttaaag gcatagggcc tataggaggc caggtcctta
    12421 ctctcataaa agacattgta aaaggcagtg aaagaagttg aaaaggccag caccagtaaa
    12481 atagcaacca tcgagctaaa ataaataggt atttcctcaa aaggaaaatg aatggctata
    12541 ttactaaaac agatgagcat caagagactg gaaaaaatgt aagaacttag gactctagca
    12601 gaaacattta cttttttccc aggattctta gcctgcttct ttcgtagatt agccagatta
    12661 gcttcttgag atgaatagag gatattgata tcaactaatt ttttaatgac cttgagacgc
    12721 atccgcaacc tcctcttttc taccagcaag actaaggtag atactttcca aaaactggtc
    12781 tgggtggtct ttcctcaagt tctctacact accacaatag atcaaatgcc cctttttcaa
    12841 aatagcaatc cgatcacaga cttgctcggc tacctcaagg acatgagttg aaaacaagac
    12901 cgtcttgcct ttttgtgcat gttccttcat catttgcttc aaatcaaagg cggcctgggg
    12961 atccaaacca gtcaagggtt cgtccaaaac ccaaatatca ggagcagaca agagtgcccc
    13021 aatgacaaag actttctgac gcattccgtg agaaagagtt tcaataacct gatagcggtt
    13081 ttcagcaaaa tcaaaaacgt tcaatagcct agctagacta gtctccaagt cagagctagt
    13141 cagatcatag gatgaagcga tcaattccca aaattcattg gccgttaagc gtaaaaataa
    13201 gtcaggcgag tctgctacgt agccaatctt tcgtttgatc gccaagcgat tttccgataa
    13261 ctcctgaccg tctaccaaaa tacgaccact gctgggtgaa atgatactga ctagggattt
    13321 tatggtggtc gattttccag ccccattatg accaatcaag cccataatct ctccattttc
    13381 aatctgcaaa ttgagattac tcaaggcctc tttatcacca tacaacttac taacattttg
    13441 aaattcaatc atgggacaac tcctatcttt atctgtatta tacatcgcta tactagcact
    13501 ttgatacaaa aaaatcaata ctttatttta agtagttaaa atagtttcct tctatcttac
    13561 gcaaacgttt gcgccgagag atactttatg atagaataaa gaacaagatt gacaagtaag
    13621 aggaaacatc atgcaaaatc aaacactcat gcaatacttt gaatggtatc tgccccacga
    13681 cggtcaacac tggacgcgtc tggctgaaaa tgctccacac ctagctcatc tggggatcag
    13741 tcacgtctgg atgccaccag ccttcaaggc aaccaacgaa aaagatgtcg gctatggggt
    13801 ctatgactta tttgacttag gagagttcaa ccaaaaaggg actgtccgca ccaagtatgg
    13861 tttcaaagaa gactatcttc aagccattca agcccttaaa gaacagggaa ttcaacctat
    13921 ggccgatgta gttctcaacc acaaggctgc tgccgataac agggaagcct ttcaggttat
    13981 cgaagttgat cctgtagacc gtacagttga acttggagaa cccttcacca tcaatggctg
    14041 gactagtttt accttcgatg gtcgccaaga tacctataat ggcttccact ggcattggta
    14101 ccacttcacc ggtacagact acgatgccaa acgcagtaaa tctgggattt atctgatcca
    14161 aggggacaac aagggctggg ctaacgagga attggtcgat aacgaaaacg gaaactacga
    14221 ctacctcatg tatgccgacc tagactttaa acatcctgaa gtcatccaaa acatctatga
    14281 ctgggctgat tggttcatgg aaacgactgg tgtagctggt ttccgtttgg atgccgttaa
    14341 gcatattgac tctttcttta tgcgcaactt catccgcgat atgaaggaaa aatacggtga
    14401 cgatttctat gtttttggtg aattttggaa cccagacaag gaagccaatc tggactatct
    14461 cgaaaaaacg gaagaacact ttgaccttgt cgatgttcgt ctccaccaga atctctttga
    14521 agccagtcaa gctggcgcaa actatgacct tcgtggcatt ttcacagata gcctggttga
    14581 actcaagcct gacaaggctg tgacttttgt cgacaaccac gatacccaac gaggacaagc
    14641 ccttgagtct accgttgaag aatggttcaa gccagcagcc tatgccctca ttttgttacg
    14701 ccaagacggc cttccatgtg tcttttacgg agactactat gggatttcag gccagtatgc
    14761 tcaagaagat ttcaaagaaa tccttgaccg cctcctagcc atccgaaaag acttggccta
    14821 tggagaacaa aatgactact ttgaccatgc taactgtatc ggttgggtac gttcaggtgc
    14881 tgaaaatcaa tccccaatcg cagtccttat ctcaaatgac caagaaaaca gcaagtcaat
    14941 gtttgtcggt caagaatgga ctaatcaaac ctttgtagat ttacttggta accaccaagg
    15001 tcaagttaca attgatgagg aaggttatgg acaattccct gtctcagcta gatccgtaag
    15061 tgtctgggca gtcaatacca tctaatagct cataataacc aagctaggtc caagcggatt
    15121 tggctttttt gtattcacaa aaagacctac ccaaatggat agatctttac ttgattacaa
    15181 tttacctgct actgcatcca acaattcttg gatcttagct tggttgcttc ctcctgccat
    15241 ggccatgtct ggtttaccac caccacgtcc atcgatgatt ggtgctaatt ctttgacaag
    15301 gtttcctgca tgaaggtctt ttgtcttgct tgctacaagg acattgactt tgtcaccgat
    15361 agcggcaact aggacaagaa gatcagagta gtctttttgt ttccagttat ctgcaaaagt
    15421 acgaagggca ccggcatcgg atacagacac ttgactagca atgtaacgat gaccgttgac
    15481 ttccttagca tctttgaaga tatcgcctgc ggctgcagct gcggcttttt ctttcaactc
    15541 agcattttct ttttgaagtt gacgaagttg ttcttgaagt ccttctacct tgtgaggtac
    15601 ttccttgact tgaggtgctt tcaaggttgc tgcgacagct ttaagagcat cctcttgttc
    15661 acgataggct tcaaaggctt ccttaccagt cactgccaag atacggcgag ttcctgaacc
    15721 gattccttct tctttgacaa ttttgaagag accaatctca gaagtgttgc caacatgagt
    15781 accaccacaa agttcaatag agtagtcacc gatagtcacg acacgaactt ccttgccgta
    15841 tttctcacca aagagggcca tagctcccat ttctttagca gtgtcaatat ccgtttcaac
    15901 tgtcttcact tcaagtgctt cccaaatttt ctcgttaact tgctgttcaa tcgcacgaag
    15961 ttcctcagca gttactgctt ggaagtgggt aaagtcaaag cgaaggaatt caacttcgtt
    16021 aagagatcct gcctgtgttg cgtggtttcc aaggatattg tgaagggcag cgtgaagcaa
    16081 atgagtcgca gtgtggtttt tcatgacacg gtgacggcga ttgctatcaa ttgccaaggt
    16141 atattcttgg ttcaaggcaa gcggtgcaag gacttcaact gtatgaaggg cttgaccatt
    16201 tggggctttc tgaacattgg tcacagtagc cacaatctta cctgactcat ccaagatttg
    16261 tccgtagtca gctacctgtc cacccatttc agcataaaat gacgtttccg caaagataag
    16321 agaggcagtt ccttctgaaa cagctcctac ttctgcattg tcagcaacga tagctaccaa
    16381 tttagaagac aattggctag tattgtagtt gaagacactt tctacagtga tgttttgaag
    16441 agtttcattt tgcataccca ttgagccacc cttgacagct gacgcacgcg cgcgttcttg
    16501 ttgttctttc atggctgctt caaaaccttc acggtctaca gtcataccag cctcttcagc
    16561 aatttcttca gtcaattcaa ctgggaaccc ataagtatca tagagtttga agacatctga
    16621 accagcgata acagattgac ctttttcttt caagtctgct acaatgcctt gggcaaagtg
    16681 ttgacctgag tgaagggtac gggcaaatga ttcttcttcg ctcttaacga ttttctcaat
    16741 aaagtcacgt ttctcaagca cttctgggta gtagctttcc atgatttttc caacagttgg
    16801 aaccaatttg taaaggaaag gctcgttgat tcccaatttt tgaccatgca tagaagcacg
    16861 acggagaaga cgacgaagaa cataaccacg accttcattt cctggaaggg caccatcacc
    16921 gatggcaaat gaaagtgaac ggatgtggtc agcgatgacc ttgaagctca tgttgtcgcc
    16981 atcttggtca taaaccttac cagacaattt ctcgacttca cggataatcg gcatgaagag
    17041 gtccgtttca aagttggtct tagccccttg gataacggcc accaaacgct ccaaaccagc
    17101 gcccgtatca atgttcttat gtggcaattc cttgtattcg ctacgaggaa cagcagggtc
    17161 tgcgttaaat tgtgacaaaa cgatgttcca gatttcaata taacggtcgt tttcaatatc
    17221 ttctgcaagc aggcgaagac cgatattttc tgggtcaaag gcttccccac ggtcaaagaa
    17281 gatttctgta tctggtccag aaggtcccgc accgatttcc cagaagttgt cctcaattgg
    17341 aatcaagtga cttggatcca ctcccacttc aatccagcgg ttgtaagaat ctttatcgtc
    17401 tggatagtag gtcatgtaaa gtttttcagc agggaaatca aaccattcag ggcttatcaa
    17461 aagctcataa gcccaagtga tagcttcgtc acggaagtaa tccccgatag agaagttccc
    17521 caacatttca aacatggtat ggtgacgcgc agtcttccct acgttttcga tgtcgttggt
    17581 acggatagcc ttttgggcat tggtaatacg tggattttca gggataatgg tcccgtcaaa
    17641 gtatttctta agggttgcta ccccagagtt gatccacaaa agagttgggt catttacagg
    17701 aaccaaactt actgatggtt ctactgagtg acctttggtc gcccagaaat caagccacat
    17761 ttggcgtact tgtgcactag atagttgttt catattgtct ccttatttac ttgtttaatg
    17821 tgattggctt tccagcattt ccacatagtc aatcgcgaca cagagggaaa tgactaggtc
    17881 tgcataagcg tcttcaagaa ccgttacggt ataggtagag gtcagatgga agagttcctt
    17941 cttaatttcc gcaatcaact gatcgcgatc atccagcaat ttgaaattca aatcccagat
    18001 attgccctcg atacgaagac ctagattatc aaactcatac ttatctcgcc aaaaggtcaa
    18061 cttcttacga atgacaaaac tcgagccatc cagaagctga atctcaaaac gaggaagcaa
    18121 ggtcaagatt tctttactga tctgactgac ttgttcacca gccgcatcat agatggtaaa
    18181 agttttggga atcttaaaaa atgatccctc cacctgatag gcaatttctc ccctgtcatc
    18241 cttgatagcg aagcgttcgc ctccaagacg aaacttttgt ttgacaagaa atgttttcat
    18301 caacacctcc aaaaatcaaa agacaagctc atatcacgaa gggcgaaaaa ccgcggtacc
    18361 accttcattc aatgaacttg tcattctctt gttcttatgc aattgtatga ttgagtagca
    18421 tgacttccta gcttagatgg ctcgcagcac cgccatttct ctggactaag acaagtgaaa
    18481 atcaattctc aactttctta ttatatcgtt tttttaatct tgcgtcaact ggaaatgatc
    18541 tccgttgaat tagaccaatt ccctacatct ctgattactt ttccaggata tattttttct
    18601 tactgccgtt tttcttttta tcccaaattt tcatattact aaacacagct actagaatat
    18661 ttccaaatat aaaggtgcct atcacccaat atatggactc agttgttagg tattgtcgat
    18721 ccaagccatc ctttaaatgg aatagtatag cagtttggtt aacaatcata aaggttggcc
    18781 agaaactttt gttgaaaaaa gtagacattt tcattatttg ttgccgcttt ctgtaaggtt
    18841 aatactcaat aaaaatcaaa aagcaaacta ggaagctagc ctcaagctgt acttgagtac
    18901 ggcaaggcaa cgctgacgtg gtttgaagag tataggctta gtatactact aggcaagcaa
    18961 ataagcaaat aaacaactag aatagaaaaa gatagggctc taaaaactga cttctattcc
    19021 ttaaaaacga accagcttga ctgattcgtc ttcttacgtt tatctcctac ttccgataca
    19081 ttttaaactg taggaagagg tcgctatatt tccctgtcca tttatggtca aatttctcat
    19141 aaatttctag gtgtttcatg gtttcaacat cgggatagaa ggccttatct tcctttgttt
    19201 cctctgggag caattccttc gctggtatgt ttggtgttga atagccgaca tactccgcat
    19261 tttggagagc attttcaggt ttcaacataa agttgataaa ggcataggct gagtcttggt
    19321 ttttaactgt tttgggaatg accatattgt caaaccaaag attgctggcc tctgtcggta
    19381 ccacataacg tagattttca tttttttcta acatttggct ggcttcacca gagaaggtca
    19441 cgccgattgc aacgttattc tgaatcatat agcccttcat ctcgtccgca acgatagcct
    19501 tgatatttgg agtcagtttg tagagcttat ccactgtctc ttccaactgc tgcagatcct
    19561 tggagttgag gctgtagccg agggaattga gtcctagtcc cagcacctca cgcgccccat
    19621 caaagagcat gatagaattc ttatactccg gcttccaaag gtcatcccaa tgctcaggcg
    19681 cttcatctac catggtttcg ttgtagacaa ttcctaaggt tccccagaag taagggatgg
    19741 agaatttatt acctgggtca aaggactggt tgagaaactc tggtccgata ttttcgattc
    19801 cttcaatttt tgaataatca agcggaacca agaggtcttc gtccttcatc ttgttaatca
    19861 tgtattcact tggaatggca atatcgtagg tcgttccacc ctgctttatc ttagtgtaca
    19921 tggcttcgtt ggagtcaaaa gtctcgtact gaacttgaat tcctgtttct tctgtaaact
    19981 gagtcaagag ttcaggatcg atatagtctc cccagttata gataaccaat ttttgactat
    20041 ctcgactatt gattttacta tctaaatgag tcgcaattcc ccacaagaca aggataatcg
    20101 ctgcaattcc tgctaaaaat gaatagattt ttttcatgct tgctcctcct tctcacgaga
    20161 gataaagtaa taacctacaa ctaggataat actaaagaga aagactagag cagacagggc
    20221 attgatttct aaggaaatcc ccttgcgagc acgagagtaa atctcgactg atagggttga
    20281 aaagccattt cctgttacaa agaaggtcac ggcaaagtca tctaacgaat aggtgaaggc
    20341 catgaaataa ccagtaatga tagacggagt caggtaagga agcatgattt ccttgaacat
    20401 ctgaaattga ctagctccca agtcataggc cgcatgaatc atgtcgccat tcatttcctt
    20461 gagtcgaggc aagaccatca agaccacgat aggaatggag aaggccacgt gactagatag
    20521 aacggtcaaa aagccaagtg aaaacttgag ttgggtaaag agaatcaaga agctagcacc
    20581 aatcataacg tcaggcgcaa ccatgaggat attattgagt gatagaaagg cttcttggta
    20641 tttcttacga gactggtaga tgtaaatggc accaaaagtc ccgataatgg tcgctatcaa
    20701 ggctgatagg aaggccaaga aaaatgtctg agccaaaatc agcatgagtc tcccatctcc
    20761 aaacatggtt tcaaagtgag tccagctaaa acctgtaaag ctattcatat catcaccagc
    20821 attaaaggca tagccaatca agtaaaagat aggcaggtag aggaccagaa agaccagtcc
    20881 cagataaagg ttggcaaatt ttttcatcgt tctctccttt ccttagtcac ccacatggtg
    20941 atgaacatgg tcaggatgag aatcacaccg atggttgaac ccataccata gttgtcattg
    21001 gttagaaaat tctgctcaat agccgtcccc aaggtgataa cgcggttccc accaatcaaa
    21061 cgggtcagca tgaagagact caaacttggg ataaagaccg actgaacccc acttcgcaca
    21121 ccgttcatag atagagggaa gatgacatga cggaaggtct cccacttagt tgcaccaagg
    21181 tcataactag cattgatgag attattatcc atgtcgtcta agacattgaa aatcggcaaa
    21241 atcataaagg ggagctcgat gtagcttgcg acaaagataa aggaaaaatc ggtaaaaagc
    21301 aactgttgtg aaccaattcc gataaattcc aagaattggt taatagagcc attttgacca
    21361 aaaatcccga taaaagcata ggctttaagg agcaaattga tccaggtagg aaggataatc
    21421 agcatgagcc agagttgacg gtgcttgaga cgggtcaaaa agagggctgt tggatagctg
    21481 ataagcaagg tcacaaaggt cacaatccct gcataaagca ctgagttgaa actcattttg
    21541 aggtaggtca agttttgtga cgcaaaataa gatttataat tttctaaact aaactggcct
    21601 tcaatgttga aaaaggattg tccgaaaatc aagaccaagg gtgccaatac aaagagggca
    21661 atccaaagca tgtagggcac tacaaagagt ttagagcttg ttttcttcat ctctttcctc
    21721 ctcgatcgca ttgatcagac ctgcttcttg ctcttcgatt tccacgtatt cttcgatacg
    21781 agcatcgaac tcttcttcgg tttcattgag acgcatgatg tggatgtctt ctgattcaaa
    21841 gtccagaccg atttcctcac ccacgatggc cttacgggtt gagtggatca tccattcatt
    21901 tccaagttcg tcataggcga taatttcata atgaactcca cggaagagct gggtgtcgac
    21961 cttaacttgg agtttgcctt cttcaggaag ggtaatgcgc aagtcctctg gacgaataac
    22021 gacctcaaca ggttcatttg gcttcatccc accatcaacc gcttcaaagc gtttgccgtt
    22081 aaattcgacc aagtagtcct caatcatggt acctggcaag atgtttgact ccccgataaa
    22141 ggtggcaaca aagtggttga ttggctcatc gtagatgtcc acaggggttc cagactggac
    22201 aatctcgcca tcattcataa cgaaaatcca gtcactcatg gcaagagctt cttcctgatc
    22261 gtgagtgaca aagacaaagg taatgcccaa tcgttgttgt aattcacgca attcgtactg
    22321 catgtctgtt ctcaatttca agtccagcgc tgataaaggc tcgtccaaca agaccacacg
    22381 gggttggttg atgatagcac gggcgatggc cacacgctga cgttgtcctc cagaaagttt
    22441 gcggatggaa cgtttttcat aaccttccaa ctgaaccatc ttgagaactt ccgctacacg
    22501 ctgctcgatt tctttcttat caattttacg caagcgaagt ggaaaggcaa cattttcaaa
    22561 cacattcata tgtgggaaca aggcatagga ttggaagacg gtatgtacgt cgcgcttgtt
    22621 ggttggaata tcattgatac gaacaccgtc tagcatgata tctcctgtcg tcgcatccag
    22681 taaacctgca ataatgttta ggatagttga tttccccgaa ccagatgcac ctagaagggt
    22741 gtagaatttc ccttcttcca actcaaagtt gatgtctttg agaaccttgg tgttgctgtc
    22801 ttcaaaaact ttagagacgt ttttgaattc gataattggt tttttcaatt ggcataaatt
    22861 ccttcttttt catagattaa ccgatcgggg ctctgtcagg tccccactac ctcttgcagg
    22921 gagtaaaacc acctgcatac atcttcgcta ccgataggct ttcacccaag atccggactt
    22981 ctctttcaag cgtaatacct gagtgttcct tgactttttc gataaccgat tggatcaagt
    23041 cctcgtagtc tttggccgtt ccatctgcga cattgatcat aaatcctgca tgcttttctg
    23101 acacttctac gccaccgata cgatagcctt tcaagccagc ttctgaaatt aactgacctg
    23161 caaaatgccc gactggacgc ttaaagaccg agccacaaga tgggtattcc aaaggttgct
    23221 tgagttcacg taggtgcgtc aagcggtcca tttcctgctt gataacctga tgggttcctg
    23281 gagctagggc aaatttaact gacaagacaa ctgcaccaga ctcctgaata gctgaatggc
    23341 ggtaaccaaa agccaagtct ttagcagaca gggtttcgat ttctccatcc ttggtcaaga
    23401 ccttacaaga ctgcaagatg tgagcaatct cgccaccata ggcacccgca ttcataaaga
    23461 cagcaccgcc aacgcttcct ggaataccac aagcaaactc aaagccagtt aaactatgac
    23521 ggagggcaat gcgagttgtt tcaatcaagt tagccccagc ttctgcttca atggtatagc
    23581 catcaacaga aacgttattg agcttgtcac acaagatgac aaatccacga atcccaccat
    23641 cacgaacgat gatattgctt gcattgccaa gaaccatcca agggatattt tcttggttgg
    23701 caaatttcac aaggcgagcc aactcaaaac gatttcgtgg aaagaccaaa taatcagcct
    23761 ctccacctac ttttgtataa ctatagctat gcaagggttc ctgaaaacgg atatcaattc
    23821 cttctaagat ttcaagcatt ttttctctta cagacatgtc actcttcctt ttccaaaatt
    23881 cattccatta taccattttt agagacattt gacgaccata aaaatacctt gtttggattt
    23941 tgcataagaa aaagaggttc cccccttttt atgatttttt acaaaagatt tccttggttc
    24001 cataggcgac cagaacgagc tccagtgcta gaatcacttc aaccaagact ggatttgtca
    24061 accagcctac ttggaaaaga gatggtgcca gatcaaagaa ggcatgcaag ccataggctg
    24121 ttaggagata aatccatttc ttctggcgaa cagcttggta aacccaaact gtcaaaagta
    24181 attggaaacc aagcgccaag attcgctcaa aaccaagcaa ataaatctgc cagaccgaaa
    24241 gtgactgaat ggtttttaac atattttcag acagtaattg cataacctgt ggattctgag
    24301 tttgaactgc cgaaagaaca atgtaaagat tgagtaaact agtaaggcct agaaaaatca
    24361 actccaagcc accatgcccc agtccatagg ccaaggcgtc tgccttttcc aaacttctct
    24421 ttttctcaaa ccatttgaag aaaacaagac gagccgtttc ctcaaaaaat gctgccatgg
    24481 ctaggccata gataatatag ataagcggat gatcttgcaa gagggcaata ctaccgtctt
    24541 tttgagggtg caaaatcaag atatgcacca gtttttccaa aatctgcgaa gagacaaaga
    24601 aagcgactgc acccaagccc aagacagcta gattaatctg gtatttcttt ttggcaaacc
    24661 aaatgcttcc tatcagaaaa gctagcaaca gcaacatggt aataatgata tgaatggtca
    24721 ttttcttctc ctattctgcc ttttcaatat cttttttcat ctcgtcaaca ttgaacttag
    24781 caaacaagta ttgacggtct tggactggga aacgttggtc caactggtca actgctccca
    24841 cctcgataat gccttccttg atgacaaaat ccatgacatg tccaccgaag gtcaaatcat
    24901 ctgatatgaa gtgcagatgg taacctgcca cactgacccc atggaaaatc tcaggcgtcc
    24961 agaaaccaac aatagtcccc gcaacattgt cacgactata ttccggttga tgggttgcga
    25021 catcagcaaa cttggtatcg ggtgttgact taggaatcat acgcacatgc atatgtaaaa
    25081 attccccccg aatcttgata gagcggaaaa gattttcccc atcataataa gactcaattc
    25141 gttctttcaa ttccttgtct gtcatctcaa agcgctggcg gaaaatgacc tctgcctgat
    25201 gcggtaccac tgcagcgtaa ggaataaggg catctggtga cacttccaca atttctggct
    25261 ggtctcctga ccctttggcc tgataagcct tgccatccaa gacaatcaat tccccatcaa
    25321 tggaatccaa ggttcccaaa ccaaggtcac catgttctag caattctcct actgtcatag
    25381 taccaccata aaggccagcc atcaaggctc caagagtatt gtattgaaat aatttcactg
    25441 gttcctgcac gttcttatcc attcactttc ttgtctgtta ttctataaat cttactccta
    25501 agtataccac atttgcccct agatgtgaac gagagaaacg ttctagacat tgccaagaag
    25561 gaaaaaaaag ggtacaatgt aacaaaatca agggaggtct ggaatgaaga aacaaagcaa
    25621 gtacaaagag gtcgtttcct atctgaaaaa tggtatcgag tctggacgat ttccgacggg
    25681 tagtcgcctg ccttctatcc gtcaactgag ccttgacttt cactgcagca aggacaccat
    25741 tcaacgagcc ctgctggaat tacggcacga acaatacctc tatgccaagc ctcagagtgg
    25801 ctactatgta ttagaacaag ggcaacatca agacctagaa atcgaggtta ccgacgaaca
    25861 tgccagtgcc tatgacgatt tccgactctg tgtcaatgaa accttgattg gccgagaaaa
    25921 ctacctcttc aactactatg acaatcaaga aggattagaa gacctaagac agtccattca
    25981 caaactcctc tttgagcaag ctctctactg caaggctaac caactagtac tgacttctgg
    26041 aacccaacaa gccttattta tcctctctca aatatccttt cctagacaag ccaaggaaat
    26101 cttggtggaa cagccaacct accatcggat gaatcgcctc ttgattgcac aggggctgga
    26161 ctatcaaacg attgaacgag gcattgatgg gattgacttg gaggagctgg aaggccactt
    26221 caaaacagga aaaattaaat ttttctacac cattccccga tttcactatc ctctgggaca
    26281 ttcctattct gagcaagaca aacgatctat tcttaactta gctgccaagt atgatgtcta
    26341 tatcgtagag gacgattatc tgggtgattt ggactccaag aagggccaaa ccttccacta
    26401 tcttgataca gaggagcatg tcatttatat caagtccttc tcgaccagcc tttttcctgc
    26461 ccttcgtatt acagcactca ttcttccaaa tgctatcaaa gaagcatttg tggcctacaa
    26521 aaatatccta gactacgaca gcaacctcat tatgcaaaag gccctgtcac tctatattga
    26581 cagtcaattg tttgaaaaaa atcgtttggc tcgcttgacc aatcatgaat cttaccaaaa
    26641 acaaatcgag gaaaggataa ctaaaacacc ttgtcccctt cctcattatc ccctacacga
    26701 tggtttattg ctagacctga gacagtatcc taaaatcgcc agtctcaaac acagtcaact
    26761 gggcttggac ttctttgaag aggcctattt gagcacctgt ccttatcaat ttgccaaggt
    26821 gtccctagac aatctggaaa atgttttaaa ctatttaaaa gcagaattgg aatgacatcc
    26881 aactctgctt tttcttattc ttcaacttca gttagacttg ctgctttttc aagatagctt
    26941 tggtaagttc cttcatcctt aagtttttgg atgaccttgt ctaccacttc tttcaaatca
    27001 tcactatttt ttctaagagc aacggcattg gcgtcgccgt ccttcatctt caagctgaca
    27061 gttgcgacag ctaagccagc gtttttagca gcataactaa gtgcaacagg ctcatccata
    27121 tgaacagcat ctatttttcc agcctgcaat tcattgactg cttcacccat attagttagg
    27181 gaagttaatt gagcttttgg caattgttcc ttgaccattg attctggaac agtccctttt
    27241 tgggctgcaa tattagcact ttctaggcta gttaaatcct tgtatttttc cacatcagcc
    27301 ttacgaacca agaaactaat cttgttttca tagtatggga ttgaaaaatc aaagacttct
    27361 tttctctcgt cagtagcact aattcctgca actgctaggt cagccttacc agtttgaaga
    27421 ctggtcaaaa cattgtcaaa actcatgctt aagatttcca acttaacccc aagttcatca
    27481 gcgatagcct gagccatgtc gatgtctgca ccgactacct ggttctttcc atcaaccaat
    27541 gattgaaatt caaagggtgc atagtcagga ctggttgcca caactaattt ccccttttgc
    27601 ttaatggcct caacagctga ctgagaacca ttagaacctg actggcaagc tactaagaag
    27661 aaacttgcaa gaaaattaca taaaacaaat atccattttt ttgatttcat aaataaacaa
    27721 cctttctgtt aattttgtat aattataacg atatccaagt tacttgtcaa gtgtttttta
    27781 aatttttatc tcaaaaatat tttttcgttc aaaaaaagga gccatcagtt gatttcaagc
    27841 tcctttttat acagaattaa actattttat agttcgacaa tcttacctgt ttcaaagtag
    27901 acaacccatt cacagatatt tttagcatag tcaccgatac gctccaagta ggaaataact
    27961 tggaaataat cacgacccgt aacaatggct tctggatttt tcttaatctc ttcagtcgca
    28021 aggtcacgga tagtttcaaa atagtggtta atttgctcat ccatggaggc cacccggtat
    28081 gcgtcgtcaa cagaaccatt aagataaaga tcaagtgctg cttccacaac gcttttaact
    28141 tcacgtccca tttttttaat ttcttcctct acagctggga tgcgttcttc ccccttcata
    28201 cggatggttg cctgggcaat ggctacagcg tgatccccca tacgctccac atctgataca
    28261 gccttaagga cagtcaagac tgtacgcaaa tcttgagaga ctggttgttg gagtgcgatc
    28321 atttcaaatg atttcttttc cagtttcact tcgtattcat ttacttctgc atcatcttcg
    28381 atgacctctt ttgccaggtc acggtcatgc gtgacaaaag cacgtaccgt acgattgatt
    28441 tgtgagagca cttcttgtcc catagcgtag aactggttat gtaatttctc taaatcttct
    28501 tcaaattgag atcgtaacat ctttcatctc cttatccaaa ttttcctgta atatagtctt
    28561 ccgtttcctt gtgttgggga tcaaggaaca tctgcttggt atcattaaat tcaatcaaat
    28621 ctccatctag gaaaaatcct gtcttatcag agatacgtga agcttgctgc atggaacggg
    28681 ttaccagaag catggtgtac ttgtctttta gaccatacaa ggtttcctca attttaccag
    28741 ctgaaatcgg atccaaagcc gaagttggct catccaagag gatgatttta ggactagttg
    28801 ccaagacacg ggccacgcag acacgctgct gttgaccacc tgacaatcca atagctgaat
    28861 catatagacg atccttgacc tcatcccaga tagaggcacc ttgcaaggct ttttctacgg
    28921 cttcatccag aacctgctta tccttaattc cattgatacg aagcccgtag acaacattct
    28981 catagatagt catagggaaa ggattaggtt gttggaaaac cattccgatt tccttacgta
    29041 attcaaccgt atctgtacgc ggactgtaga tgttgtgacc attgtacacc acggatccag
    29101 ttgtggtcac ctctggattg agatctccca tgcggttgag agacttgagg agggttgact
    29161 tccctgatcc agatggacca atcaaggctg taatttcctt aggttggaaa gatagggaaa
    29221 cactattcaa agccttcttt ttattataat atacggacag gtctgatacc tgtaaaatcg
    29281 catctgtcat acggtttcct ttctaaccaa agtgaccaga tacatagtca ttggtggact
    29341 gtagcttggc attttggaaa atagttgcag tcttgtcata ctcaatcaaa tcacccaagt
    29401 aaaagaagcc tgtatagtca cttgcacgag cagcctgctg catattatgc gttacaatga
    29461 tgatggtaaa gtttttcttg agctcaaaca tggtctcttc tagttgcatg gtcgcaatcg
    29521 gatccaaggc tgaggctggc tcatccatta agaggatatc tggcttaaca gagatggcac
    29581 gagcgataca gagacgttgt tgctgaccac ctgataaggt caaggctgac ttgtggagat
    29641 cgtctttaac ctgatcccag agggcagcct gacgaaggga ggtttctacg atttcatcta
    29701 ggacttgctt atccttaact ccagcacgtt catgcgcaaa ggtaatatta cggtaaattg
    29761 acttagcaaa tggattgggg cgttgaaaaa ccattccaat gtgtttacgc atttcataaa
    29821 cgttgatttc tggacggttg acatcaattc cacgatagag aatctgccca gttactttag
    29881 caatatcaat agtatcattc atgcgattga gactgcgtaa gtaggtagat ttccccgatc
    29941 ccgacggacc aatcaaagct gtaattttat ttctttcaaa ttgcatatca atccccttaa
    30001 tggattcatt tttaccatag taaacatgga catccttagt agaaagggct actttttctt
    30061 caggaaaggt aaggatatgc ttctcatccc agttatatgt tgacatggct tctcctttag
    30121 gcagcggtta atttcttgtg tagatagctt ccgaacttac gagctccaaa gttaaaaatc
    30181 aggataaaga tcaggagcac agcggcagaa cctgctgata caatggttcc atctggaata
    30241 gtgccttcac tattgacttt ccagatatgg acagccaagg tttctgcttg acggaagata
    30301 gagatggggc tagtcacact gaggatattc cagttagacc agtcaagagc tggcgccgat
    30361 tgccctgctg tatagatcag agctgcagct tcgccaaaga tacgaccaga tgccaagacg
    30421 acacccgtta caatacctgg aagcgcttct ggaataacaa catgaaccac tgtctcccag
    30481 cgagaaatcc caagagccag accagcctca cgttgggtat ggtgaacgtg tttcaaacta
    30541 tcctctacat tacgcgtcat ctgaggcaag ttaaagactg tcaaggccaa ggcacctgaa
    30601 atgattgaaa atccatactc aaactggact acaaagatca agtaaccaaa gagacccacc
    30661 accactgatg gtaaagagga caagatttca atacaagtcc gcacaaagtt ggtaacagga
    30721 ccttttttag catattcagc caagtaaatc ccagctccca tagaaagagg tacagaaata
    30781 atcaaggtaa tgaccaatag gaaaaaggaa ttgtaaagct gaatgccaat cccaccacct
    30841 gcttgaaaag cagaagacct tccagtcaag aaagaccaag agatatgggg caagccccga
    30901 accaagatat agagaatcaa ggaagccaag attgtcacaa tgatgctagc aatcgtatag
    30961 aggacagctg ttgcaagttt atctaatttc ttagcgcgca taatttttct ttcctctttc
    31021 tttcgtaatc aatttaatca cactgttaaa aactaaactc atcaagagca gtaccaaggc
    31081 cagtgaccag agaacattat tatttacagt tcccatgaca gtgttcccaa ttcccatagt
    31141 taatatagaa gttaaagttg cagctggtgt ggtcaaggaa gttgggataa cagctgagtt
    31201 tccgacaacc atctggatag ctaaagcctc accaaaggca cgcgccatcc caaagaccac
    31261 tgcagtgaaa ataccagaac gggccgcctt caagatcaca cgccagatag tctgccagcg
    31321 agtggctccc atagcgaaac ttgcctcacg ataataacga ggaaccgcac gcaagctatc
    31381 cgttgtcata aaggttacgg tcggcaaaat catgacaaag aggacggaaa tccctgacaa
    31441 aatcccaaaa ccagtcccac caaagacact gcgaacaaag ggaacgacga cttgcaagcc
    31501 aataaatccg tacactactg aaggaatccc aaccaggagt tcaatagctg gttgcaaaat
    31561 cttcgcccct tttggtgata cttcggtcat aaaaactgct gcaccaatag caaagggtgt
    31621 tgcgataagg gctgagagaa tggtaacgat aaaggaaccc aaaatcatag gaagggcacc
    31681 aaattcttta ctagaaggat tccaagttcc tcccaaaaga aagtcaaaga tattcacacc
    31741 attgacaaag aaggtcgaca agcctttttg cgctacgaaa accaaaatca tggccacaag
    31801 gatgactatc aaagaaagac aggcaaaggt caaacctttt cctaatttct ccagacgaga
    31861 attctttgat ggaagcaaca ttttcttagc taattcttct tgattcatta ttgtctccct
    31921 tccaacactg tcacagttcc ggcagcatct ttttcaacct tcatttcctt aatcggaata
    31981 tacttcaatc ctttgacaat cccttcttgg gtctcatccg agagaacaaa attgagaaat
    32041 tctgcagcta actcattggg ctgccccaat gtatacatat gctcataaga ccacaagggc
    32101 caattattgc tacttatatt ttctggactt aagtcatagc cattcaactt catgcttttg
    32161 accgaatcat ctatataggt aagagataaa taagagatag ctcctggact ttttgatacg
    32221 attgatttta ccgctccatt tgaatcctgc tcctgacttt gcatggcaga ctgaccttcc
    32281 ataatgacag tatcaaaggt agcacgagag ccagagccgg ctgcccgatt gataacagag
    32341 atgggtaagt ccttaccacc aacctctttc caattggtta cctcacctat gaagatttga
    32401 cgaagttgct ctgtcgttag gttatcaaca tcaacctcct tattgacaat cagagccaag
    32461 ccagctaccg cgaccttgtg gtcaacaaga gcagaagcat caattccgtc tttttcctca
    32521 gcaaatacat ctgagtttcc tatatcaact gccccagact gaacctggga caagcctgta
    32581 ccagaacctc ccccttggac attgaccgtt tttccaacat ggatcgtgcc aaattcatct
    32641 gccgctactt caaccaaggg ttgcaaggca gttgagccaa cagccgttat ggattctcca
    32701 cgatcaatcc agctagcaca gcctactaaa caagccgtca gccaaaaagc gataagagac
    32761 agagcaagct tttttctttt tttcactgtt tttctcctcg aaaataatta tgaatactgt
    32821 gaatttttta agtagttctt tatgagttga cgcatgaatt cttaccaaat ttctgcgcaa
    32881 ttgattattt atataatata ggctatatta ctctttccta acctcctttt ttcatatgtg
    32941 gataaaatct cttgtctatc ccttccccca ttgtcaccca ttatagtcat ttcgtgtctc
    33001 tttttcccct ttttaatgca agggaaatta ctctccttag atgataatcc aaaagctaga
    33061 aaggtatctc aaatctctct actctcccag actagtttac aactaaaagg aaaagattct
    33121 attttatgag aaatctagtt tacaagcggt aagaacgcta ataactaaac ttcttgtact
    33181 ctttgaaaat ctcttcaaag cagtgttttg agctatctat ggctagcttc ctagtttgct
    33241 ctttgatttt catcgagtag taaaactaca tgtgatggca atcaagatat caagaatcat
    33301 cctactaaaa aaatccatac tttcactata acatagaata agatatttga ctagcatttt
    33361 catttgaatc tgaggccttt tggaaaataa tttttcaaaa catttccagt aacctttgca
    33421 aagcccaagc cattgccttt aaccaaaact tggtaccaac catttggcag actttctgcc
    33481 agctgaacgg tttctccagc cgcatacttg acaaactctt cttggccaat ttcaaccgac
    33541 tgttcgacct gactcggttt caaggctaaa ccaagagcga aactgggctc aaagcgtttc
    33601 ttcttaaaag tacccagatg cagtccattg cgagcaatct tgagcttccc taaatctggc
    33661 aaaagttctg gcaagagata aagctggtct ccaaaaatct gcaagatacc cggtagattg
    33721 accttcaaat ggttttgggc aaattcctgc cacaaggcaa cttgttcacg gctgaggtta
    33781 ctcttacttg ccttaaattt aggagctgga ttgttaccct taaactgtag atgggcaaca
    33841 aactgaccct ctcccttaaa ctgatgaggg tacatccgag ccgtttctgg caggtcaata
    33901 ccagctacca ttccattgat atgctctact ggcaacaagt caaaatcata ctcttccagc
    33961 aaccaattga caatctcttc gttttcctcg ggtgcccagg tacaggtcga ataaaccaga
    34021 cgaccacctt cagctaacat ggtcactgca tcctccagaa tttctctttg caagctagca
    34081 cattgactcg gataatctaa gctccaatag tccatagcat caggttgctt acgaaacatt
    34141 ccttcaccag agcaaggggc atcaagaacg attaagtcaa aatagccttt aaagaccttg
    34201 accaagcggt cggcagattc attggtcacc acgacatttg tcgctccaaa acgctccatg
    34261 ttttcaacca aaatcttagc ccgtttgctt gaaatttcat tggaaacaag tagcccctcc
    34321 cctgctagat aggctgccag ttgagttgat ttgccccccg gtgcagcagc caagtccaag
    34381 accttcatac caggactggg ttgggctact tgagccacca tttgagcagc aggttcttgc
    34441 gaataaacta aacctgtagc atgctcaggc gatttccctg aaaccttccc atagtggccc
    34501 caaggggttt gagtaatggc atcagaaaag gaaagttgct cttcttttaa gggattgacc
    34561 cgaaaggccg aaaccgcttc ctcctcaaaa gaggcaagaa aatctcttgc ctcatctcct
    34621 agtatttctt tatatttttc aacaaatcct tctggaaatt tcatttaagt tcttttcctt
    34681 tcgtaaatat aggactgaat ttcctcctgc atctcaagag gcaccatcat gaccggctgt
    34741 ctggtttgaa aatcaggagc ttcaccaaaa agggtcacaa cccgatagcc cagactttcc
    34801 cctaaaatac tagctgcggc ataatcccat ggttgcagat aggtgagata ggtcaacaaa
    34861 cgccctgaca aaatcttggc aaaactaatg gccgcacttc catagacacg aacaccaaga
    34921 accgctcggc tcaaatcagc cagcccccat tcattggttt ccagcatacc actattccct
    34981 gcaatgagaa aatctccaag tggtttagtt ttaaaagaag ctaggggcct atcatttaga
    35041 caaactggaa attccccacc accgtggtaa caatcccctt tgaccacatc ataaatcaga
    35101 ccaaactgtc cctgaccatt ttcaaaataa gccatcataa cagcaaaatc ttcctgctgg
    35161 gctacaaaat tattggtacc atcaatggga tcaatgaccc aaaccttgcc ctcttgaacc
    35221 gaggctcgca gacaaccttc ttcagcacaa atcttatcct caggataacg ggacaaaatc
    35281 tcaccaacca agagttcctg aacttctttg tccagtctgg tcaccaaatc tgttggagag
    35341 gacttggttt caacacgcaa gtcttcctgc atatggtcaa gaatgtactg acctgctttc
    35401 ttaacaagct ctttagcaaa ttcaaattta cttttcaaga gaaatctttc ctttcccttt
    35461 ttctttggca gcctgaactg ctcggtaaag tgaatagcca ctaaccgttt caaattctcg
    35521 tcccaaacgt ttctcttcgc tcttgcttgg cacgaccgct ttgaatccct tataggagtc
    35581 cagtaacttt ttagcctcta ctttgccttc ataggcagct tcaacatcat taaaaaaaga
    35641 aagcactgaa gcaagttctt cagtgctcca cgacaaatct agtgggtaac tatactgttt
    35701 gttcattaac taataccagc tctcattctt gcttctttta gttcttgctt acgataacta
    35761 cgagggagaa aagcacgaat ctcatcttca ttaaaaccga tttgcatacg cttggcatca
    35821 ataataattg gacgacgcaa aagactagga tactgctcaa tcaaatgaag caattccgat
    35881 accgaaatac tctctacatc aatattcaat ttttgaaaaa tttttgaacg agttgaaatg
    35941 atgtcatcag taccattttc ggtcaaggaa aggatgtgtt gcaattcttt tcttgttaaa
    36001 ggactggtca taatattgtg ttccacaaag ggaaccttat gtttttctaa ccaggtctta
    36061 gccttacgac atgatgtaca gctcggtgat agaaatagtg taatcatgct tttctcttct
    36121 tatctatact ttgctacttc tattatacaa aaaaataagg cgcttgacta gggattttta
    36181 gaaaaaaagc ctattttttc aggaaaaata ggctgtttgc gaacgattga cacaattgga
    36241 tttggttaat tcactcttaa cgatggtttt aaacgatata tatatttata tatgtaaatt
    36301 aaaaacatct ttcctttcac ttcctacgac ttttcagaca cagatagcca aagaagcttt
    36361 catagagggc aaaaaagagg aggaaggcat gaagaaagaa ggtctctggc aaaatcataa
    36421 taacaggatc cttggctgga tcaaaaagcc aggtatcatc tcccacaaag agaatttgat
    36481 ggaaaagagt aaagaattgg tcaaaaccaa tcaaaactcc cccaagtcca atcatcacag
    36541 gtaagactac tagagccagg agactttttc gataaagaga caaaaagtcc tttttcacaa
    36601 tcctattgac aaagacatag aaacttggca gtgtcactag agctactagc tgaaccaaat
    36661 gaaagagatt cttgaccact gcgaaatggt gcaaaccagc tgctgacgaa cgaaaatcag
    36721 gcatctgtaa gacctgacta aaaggattgg tcagataatt catcaagata tgaaaattgt
    36781 attgaatggt ttctggtttt agatagactc gattcgttaa gtttagccac tgaatctcca
    36841 taggatagaa aatccaagcc agataaatgg tcagaaggat tgagagggag aggagaaaga
    36901 gcatagagcc ccaaaaggtc aatttagttt tcatcaaaat cccactccgc aaggctagaa
    36961 accacatgtg tcggtgcgat tggcaggcca gctacttctt ctgccttggt aaaacctgtc
    37021 gtcaccaaga gcgttggaat gccattgtca atcccagccc ggatatcagt caaataattg
    37081 tccccaacca tgattaactc ttcacgttcc aaacctaagt gctcaactgc cttgtccata
    37141 atgatggcat tgggttttcc gatataaacc ggcttcactc gtgtcgctac ttcaagcagc
    37201 gtaatcagtg agccagcacc tggcaaaaga ccacgttccg tcgggatgtt gaggtcagga
    37261 ttggttccga taaaatgggc acccttttga atagcaagag ttgctgtggc aaatttttca
    37321 tagtcgactt gccaatccag accaactacc acgtaggcag gtttttcctt gtcttccaca
    37381 taaccagccg ccttgatggc ttccttgagt cctgcttctc cgacaacata gacggtcttt
    37441 tcaagcccca aatcattcat atagtcgatg gttgccaaag tcgctgtgta gacagtcgat
    37501 aggggcgtat cgatattaaa attctgagcc aacatctcct taacactctc tggagtgcgg
    37561 gttgtattgt tggttacaaa gagataggga atgtcccgct tttgcaattc atgaacaaaa
    37621 gtctctccag cagggatttg gtctttcccc ttataaatgg ttccgtctaa atcaattaaa
    37681 tagcctttat atttcatcta tttctcccta agcctttttt atttcttgcc aagtaatgat
    37741 tgcttgggca ttgataaccc catcacttgt aatttcatgc ttgctttcca gtccagtccg
    37801 ttcaacagcc gatgtaatca ccccacctgg tcgaacttcc ttgacatact tgaggttgat
    37861 tttcttggga atatagtggg tcaaaaaatc cgctcccatg acctcaaaaa tccagtctaa
    37921 gtatttactg ttattgacat gaccattcat atccaagtcg taaaaacgaa catggtaatc
    37981 cttgctgatc gactcttcca aggactcata cttcggtcca cggataagtt ttttatcaaa
    38041 atcagactgg taaggagcca caatctcagg ttcaacagca tggacttttc gactgtcgcg
    38101 gtccatgaga acaaaggtcg ccatcatatg gatgagctcc tgccccgctt cattataaat
    38161 agtaaagcga cggtagcaaa aaagtcgatt gtagctcaag gcttccgttt cgatggtaat
    38221 ttcttccgca aaacgaggca aacgaaccac ctcaatatca tattctacga taatccagac
    38281 cagattatag tcttccaaaa tggccttatc actaactccc agttcaatcg actgcatccc
    38341 tgaaacttgc agtgacagca aaattacatc tggaagtttg atatgaccgt tcatatcagc
    38401 catatcaaaa ggaattttca ttttcatttg ataagttaag cccatgatcc tactccaaaa
    38461 taaatcgttc cgctacagta tctcccaaaa agagacctct ctttgtcatg cgaacgtggt
    38521 caccctcaat ctgcatgagg ccttgttgaa ccaaatctct gacaatttct ccataaagtc
    38581 cagcaaaaga ctgtccaaat ttttcctcaa atcgcgccat ggaaaccccg gatttcttgc
    38641 ggagtcccaa gaacatttct tcttccattt gctccttttg actcaggtga tcttctgtaa
    38701 tacaagcatt gccttcctca accgcactga gataatgacg aatgggacca tgatttttat
    38761 agcgtactcc attgacataa ccagatgccc ctgcaccaat accatagtat tcagcattgt
    38821 cccagtacat gagattgtga cgactttcaa agccgggttt ggagaaatta gaaatctcat
    38881 aatgctcaaa acccgctcgc tccagctctg caatgatgta ctcaaacatc tccgcttcta
    38941 gttcctcctt aggcagaggc aatttcccac gtcgcatccg attcataaag accgtatggt
    39001 tttctaaaat caaactatac aaactcatgt ggggaatatc cagtccaatg gctttagcca
    39061 cattttcctt tacttgctcc atggtctgac caggcagagc ataaatcaaa tcaatggaga
    39121 tgttgtcaaa accagccagt ttcaggcgat cgatattttc ataaatatcc ttctccaaat
    39181 gactgcgccc aatctttttc aacatcttat catcaaaggt ctggacacct agcgaaacac
    39241 gattgacagc cgaatttttc aaaacagcta tcttatccgc atccaaatcg cctggattgg
    39301 cttcaatggt caactcttct aagacagaca agtccaagtt tttagtcaag ccattcagta
    39361 aaacctccag ttgcggagcc gacagggctg tcggtgttcc accaccgata taaagggttg
    39421 acaacttttc aatatcataa gaacgaaact cttccagcag atgctctaaa tagctgtcga
    39481 ctggctgatt tttgatgaag acctttgaaa aatcacaata ataacaaatc tgggtacaaa
    39541 atgggatgtg cacataggct gacgttggtt ttttctgcat agtaattatt ataccacaaa
    39601 gactagattc cagataaaaa tcaccatccc cagatacata gtccgtccgg agatggtgat
    39661 ggtttattct tctgttatat caatcacaat ctcttctgag tcatcaagag cttcggcttt
    39721 ttcttgccat tgctccttga gattatttaa ttgatttttt gatgcttctg tcgcttgaaa
    39781 agcataggat ttagcttgag caagtatact gtccacagtg atttcacctg actcaacctg
    39841 ttcttttgtt ttcagaacaa aatctgtagc ctgctcctta acttctgtca gtttttcaca
    39901 gacttgctcc ttggcatact ccggatcttc tctcaaatca tctagaaaat cttgagcctg
    39961 actgcaaact tgtttgccct tatcacttgt taaaaacaag gcaagagctg cacctgaaac
    40021 ggttcctaaa aggattgagg ataatttacc cataaggatt ctcctttttt attttttgaa
    40081 aaatttactt gcaagacgaa gagctgacag acttgcacca gtcttgagtg tttttgaacc
    40141 agctgatgaa gctttcttgc tcaagacacg cgcatggtca ttgaggtctg aaacagatag
    40201 agataaatct gcaacagcac tgaagagtgg atcaatcgta gccaccttga cattgatatc
    40261 atctgccaag acattgacct tagccaacaa ctcattggtg tgatgcaagg tcacatccac
    40321 atctgaagtc aaggttttaa tcgtcttttc tgtttcatcg atgacacgac caagcttttg
    40381 tacagtaatg atcagataga ccaaaaagac aatcaaagct agggcaacaa gaatatatgc
    40441 aacttctaac atttagtttt cctcctctgt aatatagtaa ggggccttct ttcgattttg
    40501 ataaataacg atcattatac cgagaccgat aaggacaact gacagccatt gggacactcg
    40561 aaggccgaag aacatgagac tatctgttcg cataccttcg atgaccatac gaccgaaacc
    40621 ataccaaatc aagtaaaagg ccgtgatatg acctcgtctg agactcttcc atttccgtct
    40681 aaaaatcaga atcaaggcaa agccaagcag attccataga gactcataaa ggaaagtcgg
    40741 ttgacggtag ctcccctcaa tatacatctg gtcacggata aagccaggta gataatccag
    40801 attatccact gttgcaccat aagcttcttg gttaaagaaa ttaccccaac gccccaaact
    40861 ttgagcaatc ataacgctag gcgccgcaat atctagaaaa tcccaagtat tgatgagttt
    40921 acggtcagca aagatataga gcacaagagc cccagttatc aaaccaccgt aaatggccaa
    40981 accaccattc caaatggcaa aaatctctcc taaattctga ctatagtaat caaatcggaa
    41041 aataacatag tagagacgag ctcctaaaat agccaaggga aaggctacta agataaaatc
    41101 taaaatatcg tctggtatga tcttctttct aggtgcttct ttcatggtca aataaaccgc
    41161 aagaatcaag cctgtcacaa tacataaggc ataccaacga atggctaggg gtcctagttg
    41221 aatagcaatt ggatcaagca ttttgcacct catttcgagc gatgagactt gtcagtcgtt
    41281 cgtcgaacaa acgggtcgca tcaaagccca tttccttggc acgataattc atggcagctg
    41341 cctcaatcac aacagagata ttacgacctg ttttaactgg aatacgaata cgaggaatgg
    41401 ctacgccaga aacttcaagt tcctctgcat tgtttccaag acgatcaaag gtcttatgcg
    41461 tatcgtaatt ttccaaatag acagcaagct gaacctgtga agaatccttg acagcactcg
    41521 caccgtagag actcataaca tcgataatac caaccccacg aatttcaatc aagtgtttca
    41581 aaatttcagc tggttcaccc cagagagtaa tctcatcctt ggcaaagata tcgacacggt
    41641 catctgctac caaacggtga ccacgtttga caagctcaag acctgtctcg ctcttaccaa
    41701 ttccactatc tccctgaatc aagacgccca tcccataaat atccatcaag acaccgtgca
    41761 cactggtacg ttctgccaaa cgagaatcca gatagctaga taactctcca gataaacgac
    41821 tggtagctgc acggctggtt aaaatagcaa tcttacattc tctagcagcc tttaacatct
    41881 cctctggaac caccaaacca cgggcaacaa tgactgctgg tgtctcagat agaaacattt
    41941 ttttcaaaac ttcataacgg ctgttggaag gcatgctgat cagataagac cactccttca
    42001 tccccaaaag ttgaatccgc tctggtgtgt agtagtcaaa atagcctgtc atttcaagac
    42061 caggtcgcgt aatatccgct gtattgattt ccttttcaag caactctggt tcaccataga
    42121 caatatctag tctaagcttt tcaatcactt cttttactaa aaccgacatc atttcctcct
    42181 tcaaatgagt tcttctctct attatatcaa attgtaggag gaagtttcag atttttgcag
    42241 gctttggagt atttatttaa aagaaaaagg actagatttc tctagccttt cattccaatt
    42301 agaatttttt acgtttacga gctacttctg atttacgttt acgttttaca gaaggttttt
    42361 catagaattc acgtttgcgt gtttcttgaa gagtaccagc tttagtaacc gcacgtttga
    42421 aacggcgaag tgcgtcgtca agagattcat tcttacgtac tactgtttta gacatttttt
    42481 tcactccctt caagttcgat atctatttca ttctacacgt aaaaggaata attgtcaagc
    42541 aaaaatgctg tttaattttg attttttctt ccatttatac aagagttgaa agcgttcaat
    42601 agaacattgc ttttttattt ttcgagtaag ctaagcgctt cagcatctgc gatgatggtt
    42661 acatcagggt gattttggag gctacttgca ggtaggttct cagtcactgg gccagatact
    42721 gttccggcaa tggcttctgc tttcgactca ccgtaagcaa aaagaataat agacttggca
    42781 tccaaaatgt ttttaatccc cattgaaata gcttgggttg ggacgtcttc aatcttggca
    42841 aagaagcgtg cattggcttc gatagtagac tggtcaagtt ctactagatg cgtttgactg
    42901 tcaaatggag taccaggctc attaaagccg atatgcccat tgcgaccgat tcccaagatt
    42961 tggaggtcaa ctggatggtc agccaaaatt tggttgtagc gttcaacttc agcttcagca
    43021 ttgtccttaa caccacgagg caagaaactt tctttaaatg gtttttggtt gaacaagttt
    43081 tcttgcatga agtaacgata agactgtgga ttgtccccat caaggcccac atactcatca
    43141 aggttgacac tggttagatt tgaaaaatca aggtcactct caacaatttc cttgtaaaat
    43201 tcaagtgggc tacttcctgt cgcaagtcct agagtttgag ctccattagc caatttttcc
    43261 ttcaaaatct caaaagcaac ttttccacct tgaacttggt tttcaacttt aataactttc
    43321 atcttttcat cctccatttc tgtctatatt tattatatgg tatagaccaa tttttgtcaa
    43381 gtgaaaacga tttaatttct aaaaaaagag cgtccaaact tgaaacgtgt tataatggat
    43441 aggattagaa cttagaaaga atgaacagat atatgaatac agctgatttt gatttccact
    43501 tgccagagga attgattgcc caaacgcccc ttgaaaaacg tgatgcctcc aaactcctca
    43561 tcgtcaaccg tgagacagga gaaatgcaag at
//
LOCUS       DAVBCG010000004        41283 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBCG010000004 DAVBCG010000000
VERSION     DAVBCG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.004"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(146..349)
                     /locus_tag="VQ495_000170"
     CDS             complement(146..349)
                     /locus_tag="VQ495_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530188.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(342..1424)
                     /locus_tag="VQ495_000171"
     CDS             complement(342..1424)
                     /locus_tag="VQ495_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2530189.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1550..2332)
                     /locus_tag="VQ495_000172"
     CDS             complement(1550..2332)
                     /locus_tag="VQ495_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2530190.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2332..3675)
                     /locus_tag="VQ495_000173"
     CDS             complement(2332..3675)
                     /locus_tag="VQ495_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2530191.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3774..5090)
                     /locus_tag="VQ495_000174"
     CDS             complement(3774..5090)
                     /locus_tag="VQ495_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2530192.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5283..6504
                     /locus_tag="VQ495_000175"
                     /pseudo
     CDS             5283..6504
                     /locus_tag="VQ495_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
     gene            6703..8277
                     /locus_tag="VQ495_000176"
     CDS             6703..8277
                     /locus_tag="VQ495_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2530193.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8541..9329
                     /gene="codY"
                     /locus_tag="VQ495_000177"
     CDS             8541..9329
                     /gene="codY"
                     /locus_tag="VQ495_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2530194.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9329..9904
                     /locus_tag="VQ495_000178"
     CDS             9329..9904
                     /locus_tag="VQ495_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2530195.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            9986..10071
                     /locus_tag="VQ495_000179"
     tRNA            9986..10071
                     /locus_tag="VQ495_000179"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10020..10022,aa:Leu,seq:aag)
     gene            10354..11484
                     /gene="ugpC"
                     /locus_tag="VQ495_000180"
     CDS             10354..11484
                     /gene="ugpC"
                     /locus_tag="VQ495_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2530196.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            11985..12743
                     /locus_tag="VQ495_000181"
     CDS             11985..12743
                     /locus_tag="VQ495_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2530197.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            12830..13342
                     /locus_tag="VQ495_000182"
     CDS             12830..13342
                     /locus_tag="VQ495_000182"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2530198.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13524..14468
                     /locus_tag="VQ495_000183"
     CDS             13524..14468
                     /locus_tag="VQ495_000183"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2530199.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14477..15154
                     /locus_tag="VQ495_000184"
     CDS             14477..15154
                     /locus_tag="VQ495_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2530200.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15252..16010
                     /locus_tag="VQ495_000185"
     CDS             15252..16010
                     /locus_tag="VQ495_000185"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2530201.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16048..17553
                     /gene="lytC"
                     /locus_tag="VQ495_000186"
     CDS             16048..17553
                     /gene="lytC"
                     /locus_tag="VQ495_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2530202.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18049..18567
                     /locus_tag="VQ495_000187"
     CDS             18049..18567
                     /locus_tag="VQ495_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2530203.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            18697..19203
                     /locus_tag="VQ495_000188"
     CDS             18697..19203
                     /locus_tag="VQ495_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2530204.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNYAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19203..19373
                     /locus_tag="VQ495_000189"
     CDS             19203..19373
                     /locus_tag="VQ495_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530205.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            19405..20637
                     /gene="clpX"
                     /locus_tag="VQ495_000190"
     CDS             19405..20637
                     /gene="clpX"
                     /locus_tag="VQ495_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2530206.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20646..21233
                     /locus_tag="VQ495_000191"
     CDS             20646..21233
                     /locus_tag="VQ495_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2530207.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21244..21624
                     /locus_tag="VQ495_000192"
     CDS             21244..21624
                     /locus_tag="VQ495_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2530208.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21676..22566
                     /gene="rapZ"
                     /locus_tag="VQ495_000193"
     CDS             21676..22566
                     /gene="rapZ"
                     /locus_tag="VQ495_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2530209.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22563..23540
                     /locus_tag="VQ495_000194"
     CDS             22563..23540
                     /locus_tag="VQ495_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2530210.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23537..24448
                     /gene="whiA"
                     /locus_tag="VQ495_000195"
     CDS             23537..24448
                     /gene="whiA"
                     /locus_tag="VQ495_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2530211.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24482..25450)
                     /locus_tag="VQ495_000196"
     CDS             complement(24482..25450)
                     /locus_tag="VQ495_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2530212.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25586..26443
                     /locus_tag="VQ495_000197"
     CDS             25586..26443
                     /locus_tag="VQ495_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2530213.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26430..27209
                     /locus_tag="VQ495_000198"
     CDS             26430..27209
                     /locus_tag="VQ495_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2530214.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27233..28585
                     /locus_tag="VQ495_000199"
     CDS             27233..28585
                     /locus_tag="VQ495_000199"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2530215.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28659..29033
                     /locus_tag="VQ495_000200"
     CDS             28659..29033
                     /locus_tag="VQ495_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2530216.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29035..29883
                     /locus_tag="VQ495_000201"
     CDS             29035..29883
                     /locus_tag="VQ495_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2530217.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30160..30927
                     /locus_tag="VQ495_000202"
     CDS             30160..30927
                     /locus_tag="VQ495_000202"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2530218.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            30939..32123
                     /locus_tag="VQ495_000203"
     CDS             30939..32123
                     /locus_tag="VQ495_000203"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2530219.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32120..33991
                     /locus_tag="VQ495_000204"
     CDS             32120..33991
                     /locus_tag="VQ495_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2530220.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34134..35318)
                     /gene="mntE"
                     /locus_tag="VQ495_000205"
     CDS             complement(34134..35318)
                     /gene="mntE"
                     /locus_tag="VQ495_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2530221.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35622..38318
                     /locus_tag="VQ495_000206"
     CDS             35622..38318
                     /locus_tag="VQ495_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2530222.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38331..39122
                     /gene="yghU"
                     /locus_tag="VQ495_000207"
     CDS             38331..39122
                     /gene="yghU"
                     /locus_tag="VQ495_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2530223.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39238..39648
                     /locus_tag="VQ495_000208"
     CDS             39238..39648
                     /locus_tag="VQ495_000208"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2530224.1"
                     /translation="MEKKIVKDILFLSQVSHPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGMQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39660..41255
                     /locus_tag="VQ495_000209"
     CDS             39660..41255
                     /locus_tag="VQ495_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530225.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        12087 a         7736 c         9451 g        12009 t
ORIGIN      
        1 ctaaatcctt cggataaatt aattgtctaa ctgtttgggg gcagtacata ttcaacacct
       61 gatactatgc gttttattgt gggaagattt tcctcatttt ctagtgaaat tgaacttttt
      121 ctaacatctt ttgatagatg acttcttaat caattatcat ttccattgtt gtttcttggg
      181 gtttcatccc ttgtctttga taaaagcgga gagcaccctc gttatcattc cagacactta
      241 gggttagatt attgcagcca atttctttag cataatagca gaaaaatgac aataaaatag
      301 cccctagata ttttgaaagg aaagcatagg ggctatatag tttaatcaaa ataaagcaaa
      361 tctttgctgg tgcttgtaaa gaggtcgaag ccatccttgg taacaacacc gcagtcttca
      421 atacgaacac cgactttacc agggatatag atacctggtt caacagagaa gcacatgcct
      481 tcttcgatga ccatgtcgtt tccttccatg atagatggga attcatggac atccataccg
      541 ataccatgcc cgagacggtg gttgaagtac tcaccataac cagctttttc gatgacctca
      601 cgggcagcgc ggtccacttc atgagcagtc acacctggct tgataaagtc aagagcagct
      661 tgttgggctt caagagtcaa gttgtaaata tctttcttga attggtctgg tttgccgaca
      721 gcgactgtac gagtcatatc tgacgcatag ccattgacca gaacacccag gtcaaagagg
      781 agaagagcat cattttcaac cttattagct gctggaatgc cgtgtggatt cgcagcatta
      841 tcaccagtca agaccatggt atcaaagctc atttcataac cttcacgttt catggcaaag
      901 tcgatttgtg cgatgatatc tgtctcagtc ttatcaagag aaatattgtc aaaaccaaca
      961 tgaacagcct tgtcagcata aagacctgca accatcattt tttgcacttc atcagctgat
     1021 ttgatgaggc gcatgcgttg gatacgagga gtgaggttgt caaactcagc agtctcaaaa
     1081 actgttttca aaccatggta tttggtcaag atgagattgt caaactcaac agcgacacgt
     1141 ttgaagtcaa gttgtggaag agcatgtttg attttttgcc atggattttc agaatcgaca
     1201 tagcccacta ctgggaagga aacggtgcta cttgcacgtt ctacttcaag agctgggaca
     1261 aagaggagag gttcctgatc cgctaggaca aagaggaaca tttggcgttc atggggatca
     1321 ctgtaaaaac cagtgaggta attgattgtg acggggtcag atacgacagc gacgtctagt
     1381 ttttctgatt caagatatgt taggatttgt tgtaatttag acatatgcta ccttctttct
     1441 acccctctat tttggcaaaa aatgagagaa aatgcaaggg agaagggtaa aagaaaagac
     1501 aaaaaactcc gacaagataa cggagttctt tgatatcatt ttcctttgtt taagaaaagt
     1561 cagccttgct tttgacgtca ctgtactctt cagcgatttc ttggctgaga atgtcctcat
     1621 aggcagggag ttggatgtcc tgaccatatt tcttcttgag ggcagtagta actaggcgat
     1681 aagccagatt ggcattacga gagaggatag gcccgtggaa gtaggaacca aagacattct
     1741 tataatgaac cccttcaccg accttttctt cgttgtttcc atttccatag acaacctgtc
     1801 ccagcggttt ttggtcatca gagaggaagg tacgaccttg gtgattttca aatccatagt
     1861 aggtttcatc gaaatcttca ttgtgaatct tgatgtcacc gataaaacgg ttattggtct
     1921 ggttgagcgt gtagtgtccc atgaccccta gcccttcgat acgttttcct gaagcttcaa
     1981 cataatattg acccaatagt tggaaaccac cgcagatagc cagaactaca ccgtcgtttt
     2041 ggatgtagtt gtcaatgctc tcttttttag caggtaggtc gtctgcaatg atactttgtt
     2101 caaagtcttg accaccaccg aaaaaggcga tgtcgtagtg attttcatca aagtcatcat
     2161 ggagagaaac gatgtcaacg gtcacatggg ctcccagttt ttcagccaca tacttgagca
     2221 tgaggatgtt tccattgtcc ccgtaggtat tcatgagatt tccgtagagg tgggcaatgt
     2281 tgagctgata ggggtaattg ctatcttttg aggaaagtga agtataaacc attagttcat
     2341 ctcctttcta acaatctgac gactagccag cagttcacga aattccagca tggcagtata
     2401 agttgccaga atataggcat gcttgcagtc ttgattctca atggtcttga gaacttgctc
     2461 cagattactc gtttcagtga ttttctcagc tggatagcca gtcactcgga ggcgacgagc
     2521 gatttcagaa tgacgaacac cgccagcgtt gatttcagga atgtccatgt cagtgatttg
     2581 ctcaaagtct gcatcccaga tccagctagt gtcaattcca tctgcatagt tggcattaag
     2641 gaggacagat aggctaaatg gataaggtgc tagtttgatc atttcgatag cttgggttgc
     2701 accgactgga tttttaatca agacaagggt acattccttg tcaccgatat gaaaggtttc
     2761 ttggcgtcca aagacagcac ggctcttgtc aaatccctgt ttgatgagtt gggaatctgc
     2821 gccgaggaaa cgggcgatgg ccacagcagc tagggcgtta tagatattat agagcccgcc
     2881 gatttggata ccgtattctt ggccgtctat gacaaagcga gagcgattgt tggtcaactc
     2941 aaccagtttt gtcaaacgat agtcgagatc aggacgttta catccacaac cttcacagat
     3001 ataggcaccc aagtttgcat aggtattatg ctcatatttg aggatgcctt ggcagtcagg
     3061 acagagaatc ccttcggtat tgtagtgagc cagttgggct ggtccttttt ccaagtcaaa
     3121 accaaaatac tctatagggt ttggaatagt tggcttgtag aaaagtggac tgtctccgtt
     3181 aaggagaaca gtagcagttg gaacttttcg aatggcatcc aatatcatgt tataggtagt
     3241 atagatttca ccgaaacggt ccatctggtc acggaagata ttagtaatga caaaaagact
     3301 aggctggata tagtcacaga tacgagatag actggcttcg tcaatttcga ggacggcaat
     3361 atttttccca gtttttgaag atttggctgt taggaaggtt gttgcaatcc ctgtaatcat
     3421 gttggcaccg cttgggttgg ttagaacttg accataaacc tcttttaaaa tgccgacagt
     3481 gagggcagtt gtcagggttt ttccatttgt tccagtgaca acgacaatct cgtagttctt
     3541 agctaggctt tgtaaaatat ctttatcaaa ttgaagggcg actttccctg ggagcgtact
     3601 tccacgtcca agacggctta aaacgaagtg ggaagaacgc ccagcaagaa ggcccaaagt
     3661 agtttttaag ttcatgtttc tattatatca caaaagaggg aaaagacaga tttggtgttt
     3721 cgtagaagta aaaacagccc aaagagggct gtcagttaag atgagatttc aacttaaatc
     3781 gcaaacaagt cattcaagtt ctcagccaag gttgggtgag tgaagatttg ttttgtgaag
     3841 taagtgtaag gaatcttgtt gtccatagca acagtaatga tgttgatgat ttcttgagaa
     3901 ccttctgaga agatgcttgc accaagaatt tcttttgttt cagtattaac aacagctttg
     3961 aaagctccgc gaaggtctcc atttacgtga ccacgaggca tggctgcaac agggatttct
     4021 ttcactgcgt atggaagttt caaatcagct gcttggcttt cagtcaaacc aacttgtgaa
     4081 agtgcaggtg tgatgaacat agtatttggc acattgagac ggtcctcaag tgtgtagctg
     4141 ccatctccag caaggtagct gtaaacaaca cggaagtcat caagtgaaat gtaagtaaat
     4201 tgaaggccac cgttgacatc tccaactgca aagacaccag gaacgtttgt ttgacagtgt
     4261 ttgtctactt taatagcacc acgttcagtt agttcaatat ctgtattttc aagttgaagt
     4321 ggttctacat ttggtttgcg tccagttgcg tagagaaggg cgtcgaaacg gtaagtttcg
     4381 tcttcagtta cgacaagcac ttggtcacca tcgtttttga tttcagtagt atggatattt
     4441 tgaagcaatt caatgccatc ttcttccatg tattgtttag caagagctgc gatggaaggt
     4501 tctgcacgag gtaggaatgt atccaaggca tctaggactg tgaccttgct tccaagtttg
     4561 ttgtaaaggc cggcaaattc aagaccgata tttccgccac caaggattcc aagtttttca
     4621 ggtaatttgt ccaagctttg gatacctgtt gagtcaaaga tgtttttgct tgtagcaagt
     4681 ccaggaattg gcaagacgtt tgaaacagca ccagtgttga tgacgattgt ttcagcagtc
     4741 agttctttct tttcatcacc agcttggatt tcgatgactt tatttgaaag gaagtgagct
     4801 tccgcatcaa agatatctac gcctgtacca gcaacagtcg catagttttt accgttgagg
     4861 cgaccagtga tcgtgttttt agtagcaatc acttcttcaa aagacaagtc cttttcagca
     4921 gcaactagca aggttttagt tgggatacaa ccaatgttga tacaagttcc accgtacata
     4981 gccttgctgc gttcaacgag ggcaactttt ttgccagctg aagccaattt acctgctagt
     5041 gttttaccag ctttaccaaa tccaataaca attaaatcgt atgttaacat ttatcttcct
     5101 cctattcgaa tttcattcta gcacaagaaa aaaacttttg cacaattctg ctacggaaaa
     5161 gacagaaaaa gataaaaacg ctttccagac aaaaaactag taattttttg acaagcttga
     5221 taaactaaaa tcctatctat ttttatataa gaaagaataa atgttcgaaa ataggaggtt
     5281 ttatgaaatc gaatcgttat attattgcct ttgctggggt cattttacac ttaatgctgg
     5341 gttcgactta tgcttggagt gtttatcgta accctattat tgaaaaaacg ggatgggatc
     5401 aggcttctgt tgccttcgcc tttagtctag caatcttttg tttgggctta tcggctgcat
     5461 ttatggggcg tttggtagaa aaatttggtc cgaaagtcat ggggagtcta tctgcttttc
     5521 tatacgcagg tggaaatatc ttaacaggat ttgcaataga ccgtcaggaa ctgtggttgt
     5581 tgtatctagc ttatggcatt ttaggtgggc ttggtttggg agcaggctat attacccctg
     5641 tgtcaacgat tataaaatgg tttcctgata aacgtggtct cgcaacaggt ttagcgatta
     5701 tggggtttgg ttttgcttct ttattgacta gtcccatagc gcaacacctc atcgcagggg
     5761 tagggattgt agaaactttt tatattttag gagcaagtta ctttattatc atgctcctag
     5821 cttcacaatt cattaagcgt ccaaatgagc aagagcttgc aattttatct tcttcaggga
     5881 aagaaaaaac agcctctttg acgcaaggaa tggctgcaaa tcaggctcta aaaagcaatc
     5941 ggttttatat gctttggatt attttcttta tcaacatagc ttgtggttta ggcttaattt
     6001 cagcggcatc gccaatggca caggagatgg ctggcttgtc tacaagtcat gcagcagtaa
     6061 tggtgggtgt tttggggatt ttcaatggat ttggtcgctt gctctgggcg agtttgtctg
     6121 actatatcgg tcgccctcta acctttagta tattactgct tgtcaatctt ttctctctca
     6181 ctttggctct ttacagattc cgttttattt gtagttgcta tgtctatttt gatgacttgc
     6241 tatggagctg gtttttcttt gattccagct tatctcagtg atatttttgg aaccaaggaa
     6301 ttggccgctc tgcatggtta tattttaaca gcttgggcaa tggctggttt agcgggacct
     6361 attttattag cagagactta taaaatggct cattcgtaca cacaaacctt gttcgttttt
     6421 ctcattttat acagtatcgc cttggctttg tcttattatc taggtcgttc aatcaaaaaa
     6481 gaaagtcaaa aaccgcttac atgattttct gtagaaatgg ggctatcttt tgctctggtc
     6541 ttgtgttata ctagataggt tgcaaagaaa acagtacttt tcttttgtgg aaaagaagca
     6601 acacgatttt atatctagtt atatgaaaat acgtcataaa aagaaaagta taaactaagc
     6661 cctatagggt ggcttcgcac cacctttaga aagaagaata acgtgaaatt taatgaatta
     6721 aacttgtctg ctgatttgct agcagaaatt gaaaaagctg gttttgtaga agctagtcct
     6781 atccaagaac aaactattcc tttggccctt gaaggcaagg atgttatcgg tcaagctcag
     6841 actggtacag gaaaaactgc agcctttggc ttgcctaccc ttgaaaaaat ccgtacagaa
     6901 gaagcgacta tccaagcttt ggtcatcgct ccaactcgtg aactagctgt ccaaagtcaa
     6961 gaagaactct tccgctttgg tcgtagtaag ggagtcaaag tccgttcagt atatggcgga
     7021 tcaagcattg aaaaacaaat taaggctctt aaatctggtg cccatatcgt ggtgggaact
     7081 ccaggtcgcc tcttggactt gattaaacgc aaggccttga aattacaaga cattgaaacc
     7141 cttatccttg acgaagcgga tgaaatgctt aacatgggct tccttgaaga catcgaagcc
     7201 attatttccc gtgtacctga gaaccgtcaa actttgcttt tctcagcaac tatgccagat
     7261 gccatcaaac gtatcggtgt tcagtttatg aaagcccctg aacatgtcaa gattgcggct
     7321 aaggaattga caacagaatt ggttgaccag tactatatcc gtgttaagga acaagaaaaa
     7381 tttgacacca tgactcgtct catggatgtg gcacaaccag aactcgctat tgtatttggt
     7441 cgtaccaaac gccgtgtgga tgaattgact cgtggtttga aaattcgtgg cttccgtgca
     7501 gaaggaattc atggcgacct agaccaaaac aaacgtcttc gtgtccttcg tgactttaaa
     7561 aatggcaatc ttgatgtttt ggttgcgaca gacgttgcag cgcgtggttt ggatatttca
     7621 ggtgtgaccc atgtctacaa ctacgatatt ccacaagatc ctgagagtta tgttcaccgt
     7681 atcggtcgta caggtcgtgc tggtaagtca ggtcaatcta ttacttttgt tgctccaaac
     7741 gaaatgggtt accttcaaat cattgaaaac ttgactaaga aacgcatgaa aggtctcaaa
     7801 cctgcaagtg tagaagaatc cttccaatca aaaaaacagg tagctctcaa gaaaatcgaa
     7861 cgtgattttg cagatgaaac cattcgtgcc aactttgaga aatttggtaa ggatgctcgc
     7921 aaattggctg ctgagtttac tccagaagaa ttggcaatgt atatcttgag tctgacagtc
     7981 caagacccag atagccttcc agaagtggag attgcacgtg aaaaaccact accgtttaaa
     8041 ccatcaggta atggtttcgg tggtaaagct aagggaggtc gtggaggccg tcgtggggac
     8101 gaccgtcgag agcgtgatcg ccgtggcaat ggtcgccgtg atgagttcaa aaaaggaagt
     8161 cgtggcaacg atcgttttga taaggaaaaa cgttaccgta aggataataa aaaaccacgc
     8221 aatactttaa gcgaaaagca aacaggcttt gttattcgta acaaaggcga taaataagaa
     8281 aaagcggttc aaaatgaatc gcttttttat ataaggagac cgaatgtgaa aagacaaata
     8341 aaattactat attatctttt ataatgttat gatacacaaa aataggcaac ttgtcaatag
     8401 aaaaggaaca atttaattaa aaatattctg gtttttaatt aaattgtaag tcaatgcaag
     8461 tcaataagga attttcagat aattattgaa aaagagacgt ttttatgtta taatgatagc
     8521 gattaaattc gaggtgaaaa atggcacatt tattagaaaa aactagaaaa attacatcaa
     8581 ttttgaaacg ctcagaggag cagttgcagg atgagctccc ttacaacgct attacgcgtc
     8641 agttagcgga tattattcat tgcaatgcct gcattatcaa tagtaaggga cgtctgcttg
     8701 gctattttat gcgttataaa acaaatacag atcgcgtaga gcaattcttc caaactaaga
     8761 ttttcccaga tgactacgtt caaggggcta atatgattta cgaaacagaa gcaaacttgc
     8821 ctgttgagca tgatatgagt attttccctg ttgagagtag agatgatttt ccagatggct
     8881 tgacgactat tgcaccgatt catgtatcgg ggattcgcct tggttctttg attatttggc
     8941 gtaatgataa aaaattcgaa gatgaggact tggttcttgt tgagattgcc agtaccgttg
     9001 ttgggattca gcttcttaac ttccaacgtg aagaagatga gaaaaatatt cgtcgtcgta
     9061 ctgctgtcac catggcggtc aatacccttt cttactccga actccgtgct gtttcagcaa
     9121 ttttagggga attaaatgga aatgaaggga agttgactgc gtcagtgatt gcagatcgta
     9181 tcggaatcac tcgctctgtg attgtcaatg ctcttcgtaa acttgagtct gcggggatta
     9241 ttgaaagtcg ctcacttgga atgaagggaa cctatcttaa ggtcttgatt tcagatattt
     9301 ttgaagaagt gaagaaaaga gattactaat gacaaaggct ttaatttcga ttgattatac
     9361 agaagatttt gttgctgata gtgggaagct gacagcaggt gctccagctc aggcgatttc
     9421 ggatgccatc agcaaggtaa ctcgattagc ttttgaacga ggagattata tcttctttac
     9481 tattgatgct catgaagaaa acgatttttt ccatccggaa agtaagttat ttcctcctca
     9541 taatctgatt gggacgagtg gacggaattt atatggagat ttggggatct tttatcaaga
     9601 gcatggttca gacagtcgtg tcttttggat ggataaacgc cattactcag ctttttcagg
     9661 gactgaccta gatattcgtt tgagagagcg tagagtgtct accgttatct taacaggtgt
     9721 cttgacggat atctgtgtcc tacatacagc tatagatgcc tataatctag gatatgacat
     9781 cgagattgtt aaaccagctg ttgcttccat ctggcctgaa aatcatcaat ttgccctagg
     9841 tcatttcaaa aatacactcg gagctaagtt agtagatgaa aatctaaatg aactttctga
     9901 gtaacctggt tggtgaaaat gaaaaaattt gaaaaaaagt gttgacaagc atcccaaaag
     9961 ttgatatact agtaaagtaa tcgatgcggg gatggcggaa ttggcagacg cgcaggacta
    10021 aggatcctgt gaccgcttta ggtcgtgagg gttcaagtcc ctctctccgc ataggaaaag
    10081 agttagctaa agctagctct tttgtatttc tctaaaaaat tagaatccag tcaaatgtag
    10141 tggagaatca gcgaaaatat gggaaaactg ttccttgttt ctgcttttgt catagaagaa
    10201 taataaagat tatcttcttc aaattctccg atattctcta aagttttgtg caagttgcac
    10261 agaacttttt tatttttttg gtcatcttgc catagaaata taaagcgttt tcatctataa
    10321 tataattatc aaaagacaaa aggagttcac ctcatggtag aattgaatct taaaaatatt
    10381 tacaaaaaat atccaaacag cgaacactat tcagttgaag atttcaactt gaacatcaaa
    10441 gataaagaat ttatcgtttt cgtaggacct tcaggatgtg gtaaatcaac tacactccgt
    10501 atgattgctg gtcttgaaga cattacagaa ggtactgcat ctatcgatgg cgtggttgtc
    10561 aacgacgtag ctccaaaaga ccgtgatatc gccatggtat tccaaaacta cgctctttac
    10621 ccacacatga ctgtttatga caacatggct ttcggtttga aattgcgtaa atacagcaaa
    10681 gaagacatta acaaacgtgt tcaagaagca gctgaaatac ttggattgaa agaattcttg
    10741 gaacgtaaac cagctgacct ttcaggtggt caacgtcaac gtgttgccat ggggcgtgcg
    10801 attgtccgtg atgcgaaagt attcttgatg gacgaacctt tgtcaaactt ggatgccaaa
    10861 cttcgtgtat caatgcgtgc tgaaatcgct aaaattcacc gtcgtatcgg agctacaact
    10921 atctatgtaa ctcacgacca aacagaagcg atgacacttg cagaccgtat cgttattatg
    10981 tcagctacta agaaccctgc tggtacaggt actatcggac gtgtagaaca aatcggtact
    11041 cctcaagaag tttacaaaaa tccagttaac aaattcgttg caggattcat cggaagccca
    11101 gctatgaact tcatcaacgt gaaattggtt ggtagcgaaa ttgtttctga cggtttccgt
    11161 ttgaaagtgc cagaaggagc attgaaagtt cttcgtgaaa aaggctacga aggaaaagaa
    11221 ttgatctttg gtatccgtcc agaagacgtg aatgcagaac ctgctttcct tgaaacattc
    11281 ccagactgtg ttgtaaaagc gactatctct gtatcagaac tgcttggttc agaatctcac
    11341 ctttactgcc aagttggtaa agacgagttt gttgcaaaag ttgatgctcg tgactacttg
    11401 caaacaggtg caacagttga gcttggattt gacttgaaca aagcacactt cttcgatgta
    11461 gaaactgaaa aaacaatcta ctaaaataaa taaaattcaa agcactacaa gaaaagatat
    11521 ctctttatca attgtagtgg agagatatca gttaatctag ggagagaaac aaaatgcttc
    11581 tctccttttt gctagggaag tcatattatg catctatatt gtgatgctct ttaatactct
    11641 tcgaaaatct cttcaaacca cgtcaacgtc gccttgccgt acgtatgatt actgatttcg
    11701 tcagttttat ctgcaacctc aaagatgtac tttgagcagc ttacggctag tttcctagtt
    11761 tgctctttga tttccattga gtattatttg tgggtaccat ctacaagtga agctatatgc
    11821 gtaaactacg tgagcaattg aattcgaact agagaggtaa taataaattt atgctatagt
    11881 tatggtgact tgtatgcttt tgattctagt ttatcaaata atagattaga attgtcagat
    11941 aatatcattt tgtgttataa tgaagaaaaa acagaggtgt tcaaatgtca gaagcaggtc
    12001 ataagttttt agcaaaattg gggaaaaaac gcttacgtcc aggtggaaag cgtgctacag
    12061 attggttaat tgcagaagga ggattgtcaa aagaaaagag aatactagag gttgcgtgta
    12121 ataggggaac tacagcaatt gagttggcac agcgttttgg ttgcaagata actgctgttg
    12181 atatggatgc tcaagcttta gaagtggcta aaaaatctgc tggaacggca ggtgttgctc
    12241 atttaatcag ttttgaaaga gcaaatgcaa tgaaacttcc ttatcaagat gctagttttg
    12301 atattgttat aaatgaagct atgctgacta tgcaagccga tcaagctaag aaaaaatgtg
    12361 taatggaata tctaagggta ttaaaacctg gaggtcttct tttgacacat gatgtgcttc
    12421 ttaaggaagc taaagagtct atcagacagg aattatcaca agcaattcat gtaaatgtag
    12481 gtcctttaac tcaagatggt tgggaacagg tgatgataga atcaggttat tgtgatgtga
    12541 aagtattgac tggtgaaatg acattaatga aattatcggg tatgatttat gacgaaggtt
    12601 ggctaggaac tttgaaaatt tgtgtaaatg cttgtaaaaa ggagaataga aagcagtttt
    12661 taactatgta taaaatgttt gctaagaata aacagaaatt gggctttatt gcgatggcta
    12721 gttataaatc gtcaaaacgt tagataatta ttgaagttaa cttttccttt tttctttctt
    12781 aaaaaatatg ctataataga gagtgaaaaa ctttgaaaga aagaaaaaga tgaatttaaa
    12841 agattacatt gcaacaattg aaaattatcc aaaggaaggc attaccttcc gtgatattag
    12901 tcctttgatg gctgatggaa atgcttatag ctacgctgtt cgtgaaatcg ttcagtatgc
    12961 tactgacaag aaagtcgaca tgatcgtggg acctgaagct cgtggattta tcgtgggttg
    13021 tccagttgcc tttgagttgg gaattggttt tgcgcctgtt cgtaagccag gtaaattgcc
    13081 acgcgaagtt atttctgctg actatgaaaa agagtacggt gtcgatacct tgactatgca
    13141 cgcggatgcc attaagccag gtcaacgtgt tcttattgta gatgaccttt tggcgacagg
    13201 tggaactgtt aaggcaacta tcgagatgat tgaaaaactt ggtggtgtta tggcaggttg
    13261 tgccttcctt gttgaattgg atgaattgaa cggccgtgaa aaaatcggtg actacgacta
    13321 caaagttctt atgcattatt aatgaaaaca gtccctaggg ctgttttctc tacactagga
    13381 tataaaaata gactataact agttagagaa aaactataat tgaaaactat atcttcttgc
    13441 agtataataa aaggactaag tgtttgagat ttgtcttcaa acacatgcaa ttattcctga
    13501 gagagtacag ttaggagagg gttatgccga ttcgaattga taaaaaattg ccagctgttg
    13561 agattttacg gacagagaat atctttgtca tggatgatca acgtgctgcc caccaagata
    13621 tccgtccttt gaagatttta attttaaatc tcatgccaca gaaaatggtc acagagaccc
    13681 agttgttgcg tcacttggct aatacacccc tacaactgga tattgatttt ctctatatgg
    13741 agagccaccg ttctaaaaca actcgttcag agcacatgga gaccttctat aaaacttttc
    13801 ctgaagtcaa ggatgagtat tttgatggga tgatcatcac gggtgctcca gttgagcatt
    13861 taccatttga ggaagtggac tattgggagg aatttaggca gatgcttgag tggtctaaga
    13921 cacatgtcta ttcgaccctt catatctgtt ggggggctca ggctgggctt tatctgcgct
    13981 atggtgtaga aaaataccag atggacagta agctatcagg tatttatcct caggacaccc
    14041 taaaagaggg tcaccttcta tttagaggct ttgatgatag ctatgtatcc cctcattcac
    14101 ggcacacgga gatttctaag gaagaggtct taaacaagac caatctcgag attttatcag
    14161 aaggacctca ggttggggtt tctattttgg ccagtcgtga tttacgagaa atttatagtt
    14221 ttggtcattt ggagtatgac cgtgatacct tggcaaaaga gtattttcga gatcgtgatg
    14281 caggtttcga cccacatatt ccagaaaatt actttaaaga tgatgatgtt aatcaggtac
    14341 cttgtctttg ttggtcttca tctgcagccc tctttttcag taattgggta aaccatgcgg
    14401 tctatcagga gacgcctttt gattggagaa agatagaaga tgatgcatct gcatatgggt
    14461 atttataaga ggaattatga catatttaga cgcttttaaa tcaggtacct tggttttacc
    14521 gagtgccctg ctcttgcatt ttaaggaact ctttccttct agcgacgatt ttctggtttg
    14581 gcaatttttc tatttgcaaa atacgacagg cttagaagaa atgtcgccaa gccagattgc
    14641 tgaaaggatt ggcaaggaaa tttcggatgt caaccagtcc atttctaatc tgacggaaag
    14701 gggactgctc cagtatcgta ctatcgaatt aaatggcgaa attgaattgc tctttgatgc
    14761 tagtttggcc ttggaacgtt tggatgacct gtttggagca gttcgttcaa gttcagacca
    14821 gctaacacct caaaaccagc tcaaggattt ggtggaaacc ttccagcagg agttgggacg
    14881 attgttgacg ccttttgaga ttgaggattt gaccaagaca ctaaaggaag atggaaccag
    14941 tgctgacttg attaaggagg ctcttcgtga agctgttttg aatggaaaac caaactggaa
    15001 gtacattcag gcgattttga gaaactggcg ccatgaagga atcaagagtg tggctcaaat
    15061 tgaggccaag agagcagaaa gagaagcaag caatcctcag ttgacacagg tatctgcaga
    15121 tttcagaaat gccatggatc tctggaagga ttaatccatg caagtaggct tgaaatccga
    15181 gtaagatttg caagctgtgt ataattgtga tagaataaat agaaaataaa ttgaaaaaag
    15241 aggtatgtga aatgtcacgt aaaccattta tcgctggtaa ctggaaaatg aacaaaaatc
    15301 cagaagaagc taaagcattc gttgaagcag ttgcatcaaa acttccttca tcagatcttg
    15361 ttgaagcagg tatcgctgct ccagctcttg atttgacaac tgttcttgct gttgcaaaag
    15421 gctcaaacct taaagttgct gctcaaaact gctactttga aaatgcaggt gctttcactg
    15481 gtgaaactag cccacaagtt ttgaaagaaa tcggtactga ttacgttgtt atcggtcact
    15541 cagaacgccg tgactacttc catgaaactg acgaagatat caacaaaaaa gcaaaagcaa
    15601 tctttgcgaa cggtatgctt ccaatcatct gttgtggtga atcacttgaa acttacgaag
    15661 ctggtaaagc tgctgaattc gtaggtgctc aagtatctgc tgcattggct ggattgactg
    15721 ctgaacaagt tgctgcctca gttatcgctt atgagccaat ctgggctatc ggtactggta
    15781 aatcagcttc acaagacgat gcacaaaaaa tgtgtaaagt tgttcgtgac gttgtagctg
    15841 ctgactttgg tcaagaagtc gcagacaaag ttcgtgttca atacggtggt tctgttaaac
    15901 ctgaaaatgt tgcttcatac atggcttgcc cagacgttga cggtgccctt gtaggtggtg
    15961 cgtcacttga agctgaaagc ttcttggctt tgcttgactt tgtaaaataa tcagtaagta
    16021 gcaaaagcta ggtggaacag cattcagatg tctgttacat tttttatagg agaggaaaga
    16081 ttgaaaacaa aaattggatt agcaagtatc tgtttactag gcttggcaac tagtcatgtc
    16141 gctgcaaatg aaactgaagt agcaaaaact tcgcaggata caacgacagc ttcaagtagt
    16201 tcagagcaaa atcagtcttc taataaaacg caaacgagca cagaagtaca gactaatgct
    16261 gctgcccact gggatgggga ttattatgta aaggatgatg gctctaaagc tcaaagtgaa
    16321 tggatttttg acaactacta taaggcctgg ttttatatta attcagatgg tcgttactcg
    16381 cagaatgaat ggcatggaaa ttactacctg aaatcaggtg gatatatggc ccaaaacgag
    16441 tggatctatg acagtaatta caagagttgg ttttatctca agtcagatgg ggcttatgct
    16501 catcaagaat ggcaattgat tggaaataag tggtactact tcaagaagtg gggttacatg
    16561 gctaaaagcc aatggcaagg aagttatttc ttgaatggtc aaggagctat gatgcaaaat
    16621 gaatggctct atgatccagc ctattctgct tatttttatc taaaatccga tggaacttat
    16681 gctaaccaag agtggcaaaa agtgggcgga aaatggtact atttcaagaa gtggggctat
    16741 atggctcgga atgagtggca aggcaactac tatttgactg gaagtggtgc tatggcgact
    16801 gacgaagtga ttatggatgg tgctcgctat atctttgcgg cctctggtga gctcaaagaa
    16861 aagaaagatt tgaatgtcgg ctgggttcac agagatggta aacgctattt ctttaataat
    16921 agagaagaac aagtgggaac cgaacatgct aagaaaatca ttgatattag tgagcacaat
    16981 ggtcgtatca atgattggaa aaaggttatt gatgagaacg aggtggatgg tgtcattgtt
    17041 cgtctaggtt atagcggtaa agaagacaag gaattggcgc ataacattaa ggagttaaac
    17101 cgtctgggaa ttccttatgg tgtctatctc tatacctatg ctgaaaatga gaccgatgct
    17161 gagagtgacg ctaaacagac cattgaactt ataaagaaat acaatatgaa cctgtcttac
    17221 cctatctatt atgatgttga gaattgggaa tatgtaaata agagcaagag agctccaagt
    17281 gatacaggca cttgggttaa aatcatcaac aagtacatgg acacgatgaa gcaggcgggt
    17341 tatcaaaatg tgtatgtcta tagctatcgt agtttattac agacgcgttt aaaacaccca
    17401 gatattttaa aacatgtaaa ctgggtagcg gcctatacga atgctttaga atgggaaaac
    17461 cctcattatt caggaaaaaa aggttggcaa tatacctctt ctgaatacat gaaaggaatc
    17521 caagggcgcg tagatgtcag cgtttggtat taagcgatga tttgaaagag ggatgtgata
    17581 gtagcaccct ctttttcttt gttttatgat agttcatcct cgagtaaatt caagttcttg
    17641 ctcggaaatg aagcttatat agtagattga atatagacaa ataccttgtg attggtaaaa
    17701 cattttagaa attcatttac ctttcctaat cgacttggtt tcatcttatt tcaatctatt
    17761 atagtattgg ggaatttctt caaaccacat cagcttggtc agttctacct gcgacctcaa
    17821 aacttgtgct ttggtcaagc tgggtttagt ttcctagttt gctgatggat ttccattgac
    17881 tataagcatc caaccctctt tttgtcttct aaagaattct taaattatca gtctattgca
    17941 acttttctca tataagttct ttgtcttgct attggttttc cttagtagta tactaaggta
    18001 gtaatcatta agaagtggtt acaaaaaata atgaatgagg taaagaaaat ggtagaattg
    18061 aaaaaagaag cagtaaaaga cgtaacatca ttgacaaaag cagcgccagt agcattggca
    18121 aaaacaaagg aagtcttgaa ccaagctgtt gctgatttgt atgtagctca cgttgctttg
    18181 caccaagtgc actggtatat gcatggtcgt ggtttccttg tatggcatcc aaaaatggat
    18241 gagtacatgg aagctcttga cggtcaattg gatgaaatca gtgaacgctt gattacactc
    18301 ggtggaagcc cattctctac attgacagag ttccttcaaa atagtgaaat cgaagaagaa
    18361 gctggtgaat accgtaatgt tgaagaaagc ttggaacgtg ttcttgttat ctaccgttac
    18421 ttgtcagaac ttttccaaaa aggtttggat gtcactgatg aagaaggtga cgatgtgaca
    18481 aacggtatct ttgcaggcgc taaaactgaa acagataaaa caatttggat gcttgcagcc
    18541 gaacttggac aagcacctgg tttgtaagaa ataaggaaac atagataaat ctgtaggaaa
    18601 tttcttacag attttttcta ttttgtaagc tattccaaac cagtcttttt ttgagttttt
    18661 tgataaaata gtactatcag tgaaaaggat ggaagcatga ctaagaaaat cgtagctatt
    18721 tgggcccagg atgaagaggg tgtgattggt aaggaaaatc gtctgccttg gcatttgcca
    18781 gcagaattgc agcactttaa agaaacaact ctgaattatg ctatcttgat ggggcgtgtg
    18841 acctttgatg ggatggggcg tcgcttgctt ccaaaacggg aaaccttgat tttgacgcgt
    18901 aatccggaag aaaagataga tggggttgct acttttcagg acgtccagtc tgttcttgac
    18961 tggtatcagg ctcaagaaaa gaatctctac attatcggtg ggaagcaaat ttttcaggct
    19021 tttgaacctt accttgatga agtgattgtc actcacattc atgctcgggt ggaaggagat
    19081 acctatttcc ctgaagaact tgacttgtct ctttttgaga cagtttcaag caaattttac
    19141 gccaaagatg agaagaatcc ttatgatttt accatccaat accgcaagag aaaggaagtc
    19201 taatggaacg gagtttattt ggtctattca cggcttttct gtgtttcatc tgctttctgg
    19261 caggagcaca agcttttcgt aaaaagcgtt acgggctttc tatcctcctt tggttaaatg
    19321 cctttaccaa tctggtcaat agtattcatg ctttttacat gaccttattt tagatagaat
    19381 gacagtatag aatggaagga aatcatgtct acaaatagaa aaaatgatat gatggtttat
    19441 tgctcatttt gtggcaaaaa ccaagaagaa gtacaaaaaa taattgctgg caacaatgct
    19501 tttatttgta atgaatgcgt ggagttagct caggaaatca ttcgagaaga attggttgag
    19561 gaagtcttgg cagacttgtc tgaggtgcca aaaccaattg aactcctcca tatcttgaac
    19621 cactatgtaa ttggtcaaga tcgtgccaag cgtgccttgg cagtggcggt ttataaccac
    19681 tacaaacgca tcaatttcca cgatacacgc gaagagtcag aagatgtgga tttgcagaag
    19741 tcaaacattt tgatgattgg cccaactggt tcagggaaaa ctttccttgc ccagaccttg
    19801 gctaagagct tgaatgtacc ttttgctatt gcggatgcga cagctctgac ggaggctggt
    19861 tatgtgggtg aggatgtgga aaatatactc ctcaaactct tgcaggttgc tgactttaac
    19921 atcgaacgtg cagagcgtgg cattatctat gtggatgaaa ttgacaagat tgccaagaag
    19981 agtgagaatg tgtctatcac acgtgatgtt tctggtgaag gggtgcaaca agcccttctc
    20041 aagattattg agggaactgt tgctagcgta ccgcctcaag gtggacgcaa acatccacaa
    20101 caagagatga ttcaagtgga tacaaaaaat atcctcttca tcgtgggtgg tgcttttgat
    20161 ggtattgaag aaattgtcaa acaacgtctg ggtgaaaaag tcatcggatt tggtcaaaac
    20221 aataaggcga ttgacgaaaa cagctcatac atgcaagaaa tcatcgctga agacattcaa
    20281 aaatttggta ttatccctga gttgattgga cgcttgcctg tttttgcggc tcttgagcaa
    20341 ttgaccgttg atgacttggt tcgcatcttg aaagagccaa gaaatgcctt ggtgaaacaa
    20401 taccaaacct tgctttctta tgatgatgtt gagttggaat ttgacgacga agcccttcaa
    20461 gagattgcta ataaagcaat cgaacggaag acaggggcgc gtggacttcg ctccatcatc
    20521 gaagaaacca tgctagatgt tatgtttgag gtgccgagtc aggaaaatgt gaaattggtt
    20581 cgtatcacta aagaaactgt cgatggaacg gataaaccga tcctagaaac agcctagagg
    20641 tgactatgga acttaataca cacaatgctg aaatcttgct cagtgcagct aataagtccc
    20701 actatccgca ggatgaactg ccagagattg ccctagcagg gcgttcaaat gttggtaaat
    20761 ccagctttat caacactatg ttgaaccgta agaatctcgc ccgtacatca ggaaaacctg
    20821 gtaaaaccca gctcctgaac ttttttaaca ttgatgacaa gatgcgcttt gtggatgtgc
    20881 ctggttatgg ctatgctcgt gtttctaaaa aggaacgtga aaagtggggg tgcatgattg
    20941 aggagtactt aacgactcgg gaaaatctcc gtgcggttgt cagtctagtt gaccttcgtc
    21001 atgacccgtc agcagatgat gtgcagatgt acgaatttct caagtattat gagattccag
    21061 tcatcattgt ggcgaccaag gcggacaaga ttcctcgtgg taaatggaac aagcatgaat
    21121 cagcaatcaa aaagaaatta aactttgacc caagtgacga tttcatcctc ttttcatctg
    21181 tcagcaaggc agggatggat gaggcttggg atgcaatctt agaaaaattg tgaggaaaag
    21241 aaaatggcaa aaacaattca tacagataag gccccaaagg ctatcggacc ctatgttcaa
    21301 ggaaaaatcg ttggcaacct tttgtttgct agcggtcaag ttcccctatc ccctgaaact
    21361 ggggaaattg taggagagaa tatccaagaa cagacagagc aagtcttgaa aaacatcggt
    21421 gctattttgg cagaagcagg aacagacttt gaccatgttg tcaaaacaac ttgcttcttg
    21481 agcgatatga acgactttgt tccttttaat gaggtttacc aaacggcctt caaagaggaa
    21541 ttcccagctc gttcagctgt ggaggtagct cgtcttcctc gtgatgtaaa agtcgaaatt
    21601 gaagtcatcg cagagattgg ataagctagt tgaagtttgg tgttgccaaa cttcttttga
    21661 tataaggaga aaaagatgac aaagaaacaa cttcacttgg tgattgtgac agggatgagt
    21721 ggcgcaggga aaactgtagc cattcagtcc ttcgaggatc taggttattt caccattgat
    21781 aatatgccgc cagctctctt gcctaagttt ttgcagctgg ttgaaattaa ggaagataat
    21841 cctaagttgg ctttggtagt ggatatgcgt agccgttctt tcttttcaga gattcaagct
    21901 gttttggatg agttggaaaa tcaagatggt ttggatttca aaatcctctt tttggatgcg
    21961 gctgataagg aattggtcgc tcgttacaag gaaaccagac ggagtcaccc actagcagca
    22021 gacggtcgta ttttagatgg aatcaagttg gaacgtgaac tcttggcacc tttgaaaaat
    22081 atgagccaaa atgtggtgga tacgactgaa ctcactccac gtgaactgcg caaaaccctt
    22141 gcagagcagt tttcagacca agaacaagcc cagtctttcc gtatcgaagt catgtctttc
    22201 ggatttaagt atggaatccc gattgatgcg gacttggtct ttgatgtccg tttcttgcca
    22261 aatccctatt atctaccaga actgagaaac caaacgggtg tggatgaacc tgtttatgat
    22321 tatgtcatga accatcctga gtcagaagac ttttatcaac atttattggc cttgattgag
    22381 ccgattctgc caagttacca aaaggaaggt aagtccgttt tgaccattgc tatgggatgt
    22441 acgggtggac aacaccgtag tgtggcattt gctaaacgct tggcgcagga cttatccaag
    22501 aattggtctg ttaatgaagg gcatcgcgac aaagaccgca gaaaggaaac ggtaaaccgt
    22561 tcatgagaaa accaaagata acggtgattg gtggagggac tggaattccc gtcattctaa
    22621 aaagtctgcg ggaaaaagat gtggaaatcg cagctatcgt gacggtggca gatgatggtg
    22681 gttcttcagg tgaactccga aaaaatatgc aacagttgac accgccaggt gatcttcgta
    22741 atgtccttgt ggccatgtcg gatatgccta agttttatga gaaggtcttt cagtatcggt
    22801 tctctgagga tgccggagcc tttgctggcc atccattggg aaatctcatc attgctggct
    22861 tgtcagaaat gcagggttca acctataatg ccatgcagtt attgagcaaa tttttccata
    22921 caacagggaa aatttatcct tccagtgacc atcctttgac ccttcatgca gtctttcagg
    22981 atgggacaga agtggctgga gagagtcata ttgtagacca tcgaggcata attgacaatg
    23041 tctatgtgac caatgcccta aacgatgata cgcctctggc cagccgtcga gtagtgcaga
    23101 ccatccttga aagtgacatg attgtcctag ggccaggttc cctctttacc tctattttgc
    23161 ccaatatcgt gattaaggaa attgggcggg ctcttttgga aactaaggca gaaattgcct
    23221 atgtctgcaa tatcatgacc caacgtgggg agacggaaca ctttacagat agcgaccacg
    23281 tggaagtctt gcatcgtcac cttggtcgcc cttttatcga cactgtcttg gtgaatattg
    23341 aaaaagtgcc tcaggaatac atgaattcca accgttttga tgaatacttg gtgcaagtgg
    23401 aacacgattt tgtaggtctt tgtaagcaag tttcgcgcgt gatttcatct aacttccttc
    23461 gtctggaaaa tggcggtgcc ttccacgatg gagatttgat tgtggacgag ttgatgcgca
    23521 ttatacaggt gaaaaaatga gtttcacagt agcagtaaaa gaagaaatcc tgggccaaca
    23581 ccatctgagc cggcatgaat tatctgccat tatcaaaatg tctggtagca tcggtctctc
    23641 gacttcgggc ttgactttgt cagttgtgac agaaaatgcc aaactggccc gtcacctcta
    23701 tgagtccttt ctccatttct atgaaatcaa atctgaaatt cgtcaccacc aacggagcaa
    23761 tcttcgcaag aatcgcgtct ataccgtttt tacagatgaa aaggtgcagg atttgttaag
    23821 tgatttgcac ttggcagact ctttctttgg cctggaaaca ggtattgatg aggcgatttt
    23881 atcggatgag gaagcaggtc gtgcctatct ctgtggcgct ttcttggcaa atgggagcat
    23941 tcgtgaccct gagtcaggca agtaccagtt ggaaatcagt tctgtttatc tggaccacgc
    24001 gcaaggaatt gcctcccttc tccaacagtt tttactggat gccaaggtgc ttgagcgcaa
    24061 gaagggggct gtgacctatc tccagcgagc agaagacatt atggacttct tgatagtaat
    24121 cggagccatg caggcacgtg atgattttga gcgggttaag attttgcgag aaacccgtaa
    24181 cgacctcaat cgggctaata atgccgagac agctaatatc gctcggacag tttctgccag
    24241 catgaagacc attaacaata tcagtaaaat aaaagatatc atgggcttag aaaatttgcc
    24301 agtagatttg caggaagtgg cgcaactgag gattcagcac ccagactact ctatccagca
    24361 gttagcagat agtctcagca cccctctgac caaaagtggt gtcaatcaca gactcagaaa
    24421 aatcaataag atagcggatg aattataaaa ccacgaatcc tatgtgactc gtggttcttt
    24481 tttataaact ggtagagtgt tttggttgta ctttttgttc agggtcaatg tagttgatag
    24541 cgttgttgac agcagttgga gcttctccga ggcctgtcgc aatcagatca atttttccgt
    24601 catagtagca gcagtcaccg atagcataga tacctgcttg gctggattcc tgtttgctgt
    24661 tgacgataat cttgtgacgg ttgaggtcga gcccccagtt tttaaggtta ccgacagaag
    24721 atttgaaacc atagttgaca aagaggtggt ctaggtcaat ggtttcagtt tcatcagatt
    24781 tgacttttgt gatttcaagt ttatcaagtg tttttccatt tccaaggagt tggctagggg
    24841 cgaatggtgt cttgatggtt acagatgatt cttgcaaggc ttgaacactg tgttccaagg
    24901 cacggaaatt atctctgcgg tgaacaaggg tagttggtgc gattttttca aaagccaaag
    24961 tccaatccac agccgagtct cccccaccaa gaatcgtcac ttttttacca gcgtattgct
    25021 gaatgttaga aacgtggtag tggatatttt catagccctc aaccccttca agttccagcg
    25081 gacgtggttt gaaggcaccg ccacccatag cgatgataac tgttttagtc aggtgacttc
    25141 ctttagaagt tgtgatggca aattcttctt cttgtttgtc aatctcaaga accgtttcat
    25201 tgagatgaat aggggtatca aatccattta gctgttcaat caagcggtta gtcaactctt
    25261 ctccagtcag gtttgggaag cctggtacgt ctaggatttc cttttcaggg tagagaatag
    25321 caggttgtcc acctagctgg ggaagagagt cgatgatttg aaccttggct tggcgtaggt
    25381 gggcataaaa ggctgcaaaa agcccgacag gaccaccacc cacaatggta atatcataga
    25441 gttgagacat agtttctcct ttgttttttc tagtcagttt attttatcat atttttgttc
    25501 aatgtaaaat ggactaggat agggaaaaaa atgccagaaa atggtataat aggacgaaag
    25561 tgttttgata gggaggatat cagggatgaa ttttcaacaa ttatccaatt tgcaatattg
    25621 gaccagtttg tttgcaagtc catggacgat agctatcaat ctgattgata ttttgattgt
    25681 tgcttatatt ttataccatt ttacaaaagc tattgcagga accaagatta tgattttggt
    25741 acgtggagtt ttggtattta ttttagctca aatccttgca aatatgattg gtttgactac
    25801 gatttcttgg ttaatcaatc aaattattac ttatggggtt attgcggcgg ttgttatctt
    25861 ctctccagag attcggactg gtttggaacg tttgggaaga gcgacagatt tcttttccaa
    25921 tgcccctatt agtgctgagg aacagatgat tcgtgccttt gttaagtctg ttgaatacat
    25981 gagtcctcgt aaaatcgggg ccttggttgc tattcagcgt gtacgtacct tgcaggagta
    26041 tatttcgaca ggaattccct tggatgctaa gatttctgca gaacttctca ttaacatttt
    26101 tattcccaac actcccctac atgacggtgc ggtgattatc aaagaagaac gtatcgctgt
    26161 gacgtctgcc tatctgccct tgacaaaaaa cacagggatt tccaaggaat ttgggaccag
    26221 acaccgggcg gctatcggtt tatcagaagt ctcagatgcc ttgacttttg tcgtatcaga
    26281 ggaaacggga ggaatttcga taacctataa tggaaggttt aagcacaacc taacacttga
    26341 tgaatttgaa acagaattac gtgaaatctt acttccaaaa gaggaagtag gtcttagttt
    26401 taaagaacga ttgctaggag gatggaaaca tgaaaaaaaa tagtttatat atcatatcct
    26461 cactcttttt tgcttgtgtc ttatttgtct atgctacggc gacgaatttt caaaacagta
    26521 ccagtgctag gcaggtaaaa acggaaacct atactaatac agtaacaaat gtccctattg
    26581 acatacgcta taatagtgat aagtatttta ttagcggttt tgcttcagaa gtatcagtgg
    26641 tcttgactgg tgcaaatcgc ctatcgctag ctagtgaaat gcaagaaagt acacgtaaat
    26701 tcaaggttac tgctgaccta acagatgccg gtgttggaac aattgaagtt cctttgagca
    26761 ttgaagattt acccaatggg ctgaccgctg tggcgactcc gcaaaaaatt acagtcaaga
    26821 ttggtaagaa ggctcagaag gataaggtaa agattgtacc agagattgac cctagtcaaa
    26881 ttgatagtcg ggtacaaatt gaaaatgtca tggtgtcaga taaagaagtg tcgattacga
    26941 gtgaccaaga gacattggat agaattgata agattatcgc tgtcttgcca actagcgaac
    27001 gcataacagg taattacagt ggttcagtac ctttgcaggc aatcgaccgc aatggtgttg
    27061 tcttaccggc agttatcact ccgtttgata caataatgaa ggtgactaca aaaccagtag
    27121 caccaagttc aagcacatca aattcaagta caagcagttc atcggagaca tcttcgtcaa
    27181 cgaaagcaac tagttcaaaa acgaattaaa aatagaaaag gattttataa caatgggtaa
    27241 atattttggg actgatggag tccgtggaga agctaaccta gaactaacac cagaattagc
    27301 ctttaaacta ggacgttttg gaggctatgt tcttagtcaa catgaaacgg aagcgccgaa
    27361 agtctttgta ggacgtgaca cacgtatttc aggggaaatg ctagaatcgg ccttggtggc
    27421 aggtctcctt tcagtaggga ttcacgtata caaacttggt gtccttgcaa caccagcagt
    27481 agcttacttg gttgaaactg aaggagcaag tgctggtgtc atgatttctg ctagccacaa
    27541 tccagctctt gataacggaa tcaagttctt tggcggtgat ggcttcaaac tagatgatga
    27601 aaaagaagca gaaattgaag ccttgctaga tgctgaggaa gacactcttc ctcgtccaag
    27661 tgcagaaggc ttaggaattt tggtagatta tccagaaggc ttgcgtaagt atgaaggata
    27721 ccttgtttca actggaactc ctcttgatgg aatgaaggtt gccttggata cagctaatgg
    27781 agcagcttct accagtgccc gtcaaatctt tgcagacctt ggtgcccaat tgacggttat
    27841 cggggaaaca ccagacggtc ttaacatcaa ccttaatgtt ggttcaacac atccagaagc
    27901 ccttcaagaa gtggtcaaag aaagtgggtc agctattggt ttggcctttg atggagacag
    27961 tgaccgcttg attgctgttg atgagaatgg tgacatcgtc gatggtgaca agattatgta
    28021 catcatcgga aaataccttt ctgaaaaagg acaattggct caaaatacaa ttgtgacaac
    28081 tgttatgtct aaccttggtt tccacaaggc cttgaatcgc gaaggtatta acaaggcagt
    28141 tactgcagtt ggtgaccgct acgttgttga agaaatgaga aaatcaggtt acaaccttgg
    28201 tggtgaacag tctggtcacg ttatcttgat ggattacaat accacaggtg atggtcaatt
    28261 atcagcaatt caattgacta aaatcatgaa ggaaactggt aagagcttat cagagttggc
    28321 ggcagaagta acgatttatc cacaaaaatt agttaatatc cgagtggaaa acgtcatgaa
    28381 ggaaaaggcc atggaagtgc cagctatcaa ggccatcatc gagaagatgg aagaagaaat
    28441 ggcggggaac ggccgtatcc ttgttcgtcc aagtggaaca gagcccctct tgcgtgttat
    28501 ggcagaagcg cctacaacag aagaagtgga ctactatgtt gatactatca cagatgtagt
    28561 tcgtgctgaa attgggattg actaaatcct agaaaactag atgaaaataa aaaattatgg
    28621 tacaatagct tataatattg tagccgatgg agaaagacat gaatcttaaa cgtgaacaag
    28681 aatttgttag tcagtatcat tttgatgcac gtaattttga atgggaaaat gaaaatggag
    28741 ctcctgaaac caaggtggat gtgaatttcc aattgctcca acatgaccaa gaaaatcaag
    28801 tgacttcctt gattgtcatt ttgagcttta tgattgtatt tgataagttt gtcattagtg
    28861 gaacaatctc acaagtaaac catatcgatg gccgtattgt caatgaacca agtgaattga
    28921 accaagaaga ggtggaaacc ttggctcgtc catgtttgaa catgctcaat cgtttgactt
    28981 acgaagtgac tgaaattgca ttagacttac caggaattaa tttggagttc taatatgaaa
    29041 ttagctgttt tcacagattc ctctgcttat ctcagtgaag agaccttgca aagagaagac
    29101 ttgtttgtct tggatattcc tgtcaatatt gatggtgagg aatatgtcga aggcatcaat
    29161 ctgtctgctg aggaatttta ccaaaaaatg gctcaggctt ctgaattgcc taagaccagt
    29221 caaccaagta ttgccaagtt agatgagatc ttaacttcgc tcaaagaaca aggctataca
    29281 catgccttgg ggcttttcct atcttctgga atttcaggtt tttaccaaaa tatccagtat
    29341 atggtcgatg actatgaggg cttaaccatt gctttcccag acactttgat tacaagtgct
    29401 cccctaggca tcatggttga aagcgtcttt aattggcgtg atcagggcga tgattttgcc
    29461 agcattcagg ataagctagc cattcaaatc agccgtacgt cagcctttat catggtagat
    29521 gacttggatc atttggtaaa aggtggacgc ctttcaaatg gggctgccat tttgggcaat
    29581 ttgcttagca ttaagccaat cctttatttt aacgatcaag gtgtgattga agtttacgaa
    29641 aaagttcgta ctgaaaagaa ggccaccaag cgcttaattg aaattatcaa ggaaacaacg
    29701 gcttcaggtc aataccgggt cattgtcatt catgggaatg ctcctgaaaa ggctgaagaa
    29761 ttgcgtcagc acttgcttga ttttggcttg ggttcggatg tttcacttgc tacatttggt
    29821 agtgtcattg gaacgcacct aggagcagga agtattgctc tgggttatat tccagtgatt
    29881 tagttggcgc ttctaggcta gttaagatgc cttgtatctt agaaattgaa cacggactac
    29941 ctgcctcttg aaaaaagatg cctgtcttgc ctttcgtgga aagtcagtgc cattccctat
    30001 ttttcagagg cagctaaggt cctttgtatc ttgataattg aacacgcctg gaaccctgtg
    30061 tgaaaaagat agttcttcca agaagtagac acttcttgat tggaattcct attttcactt
    30121 tgtgttctta cggactttgt atcttagaca ggagtagaga tgagtattcg agtaattatt
    30181 gccggtttta agggaaagat gggccaggct gcttgtcaga tggtattgac tgatccagac
    30241 ttggacttgg tggcagtttt ggatcctttt gagtctgagt cagaatggca gggtattcct
    30301 gttttcaagg ataaggctga tttagctggt tttgaagcgg atgtctgggt agattttact
    30361 actccagctg ttgcctacga aaatacacgt tttgctcttg aaaatggctt tgctccagta
    30421 gttggaacga ctggtttcac gagtgaagaa attgcagagc taaaagaatt ttctcgtgcc
    30481 caagacttgg gtggcctgat tgcccctaac tttgccttgg gtgctgtctt actcatgcaa
    30541 tttgcgacgc aggctgccaa atatttccca aatgtggaga ttattgagct ccatcatgac
    30601 aagaaaaagg atgctccgag tggaacagcc attaaaacag ctgagttgat ggcagaggtt
    30661 cgagagtcca ttcagcaagg cgcagcagat gaggaagagc tgattgctgg tgctcgtggt
    30721 gctgactttg atgatatgcg catccactca gttcgtttgc caggcttggt agctcatcag
    30781 gaagtcatct ttggcaatca gggagaaggg ttgaccctcc gtcatgactc ctatgatcgc
    30841 atctccttca tgacaggagt caatttggga attaaagaag ttgtcaagcg tcatgagctt
    30901 gtctatggat tagaacactt attatgagat taacgcaaat gccttctgaa tttcagaagg
    30961 ctttaccagt attagaaaaa attaaagaag caggctttga ggcttatttt gttgggggct
    31021 ctgttcgaga tgcccttctc catagcccta tccacgatgt ggatattgcg acgtcttctt
    31081 atccagaaga gaccaagcag atttttccgc gaacagccga tatcggaatc gagcatggaa
    31141 ccgtcttggt cttagatggg gatgaggagt atgaggtaac aacctttcgg acagaggatg
    31201 tctatgtgga ctatcgcaga cccagtgcgg tttcctttgt acgatcgcta gaagaagacc
    31261 tcaaacgccg tgatttcaca gtcaacgcct ttgccttgga tgagacagga gaaatcgttg
    31321 acttgttcca tggtttagaa gatttggaaa agcaagtctt gcgagcagtt ggagtggcta
    31381 gtgagcgttt caacgaagat gctttacgga ttatgcgtgg tttccgtttt caggctagtc
    31441 ttggttttgc acttgagcca gaaacattta aagctatgaa gaccttgacg ccgcttttgg
    31501 agaaaatttc tgtagagcgt accttcgtcg agtttgataa actcttgctg gctccatttt
    31561 ggagaagggg cttggcttcc atgattgaga gtcaagctta tgactatctc cctgatatgg
    31621 catctagcca ggacaagctc aacagactgt ttgatttgga gactgatttt acttttgaat
    31681 cctctgaaca agcctgggcg gctttactat gggctttgga gattgaaaat gcgcagtcat
    31741 ttttgaaatc ttggaagacc tcacgccagt ttgccaagca agttcaggat ttgctgatta
    31801 ttttggctct gcgtgaaaat ggagaattga gtaagcgaga ttgttatcgc tttgacatag
    31861 atttgctttt acaggctgaa aatcttcgtc aagctcaagg aaaagaagtc aacccacaag
    31921 ccatcacaga aaaataccaa agcttgacta tccatgacaa gaaagagatt cagattaatg
    31981 gcgatatttt gatcaaggaa tatggctatc agccaggccc agacttggga gagattttaa
    32041 cagagattga gtttgccatt gtcgatggag aattggagaa taatcgtgaa gccatccatg
    32101 cttacctgag ggagaaaaaa tgagtgattt tatcgttgaa aaactaagta aatccgttgg
    32161 tgacaagacc gtttttaggg atatttcctt tattatccat gacttagaca gaattggttt
    32221 aatcggtgtc aatgggactg gcaagaccac ccttttggac gtcctttctg gtgtttctgg
    32281 atttgatggg gatgtcagtc ctttttcagc taaaaatgat taccagattg gttacttgac
    32341 tcaggatcct gattttgatg atagaaagac agttttggat acggttctat ctagtgaact
    32401 caaggaaatc cagctcattc gtgagtatga attgattatg ctcgactata gtgaggacaa
    32461 gcaggcgcgt ttggaacgtg tcatggcaga gatggactct ctccaagctt gggaaatcga
    32521 aagtcaggtc aagaccgttc ttagcaaatt gggcattcaa gacttatcta ctcctgttgg
    32581 ggaattgtca ggtggtctga gaagacgggt acagttggca caagtcttac ttggcaacca
    32641 cgacctcttg cttttggatg agccgaccaa ccatctggat attgcgatta ttgagtggct
    32701 gaccctcttt ttgaaaaatt ctaagaagac cgtccttttt atcactcacg atcgttattt
    32761 cttagacgct ttgtcaacac ggattttcga gttggatcgt gcaggcttga ccgagtacca
    32821 gggcaattac caggactatg ttcgcctaaa ggcggaacag gatgagcgcg acgcggctct
    32881 tcttcacaaa aaagaacaac tctacaaaca agaactggcc tggatgcgca gacaaccgca
    32941 ggcgcgtgcg accaagcagc aggcccgtat caatcgattc catgatctga aaaaggaagt
    33001 ttcaggcagt agtgctgaga cagacttgac catgaacttt gaaaccagtc ggattgggaa
    33061 gaaagtcatc gagtttcagg ctgtttcctt tgcctatgaa aacaagccta ttttgcaaga
    33121 ttttaatctc ttggtgcagg ccaaagaccg tatcggaatc gttggggaca atggtgttgg
    33181 aaaatcaacc ctgctcaact tgattgcagg aagtcttgag ccaactaaag gacaagttgt
    33241 gattggggaa actgttcgca tcgcctattt ctctcagcaa attgagggtt tggatgaaag
    33301 caagagagtt atcaattacc tgcaggaagt ggcagaagag gtcaagacca gtggcggttc
    33361 tacgacttcg attgcagagt tgctggagca gttccttttc ccacgttcga cgcatggaac
    33421 cttgattgag aaattgtcag ggggtgagaa aaaacgtctt tatctcctca aactgctttt
    33481 ggaaaaacca aatgttcttc ttttagacga gccaaccaat gacctagata ttgcaacttt
    33541 gacagtctta gagaatttct tgcaaggttt tgcaggtccc gttttaacag tcagtcacga
    33601 ccgctatttc ttggataagg tagcgaccaa gattctcgct tttgaggata gcaagattcg
    33661 tcctttcttt ggtcattaca ccgactatct tgatgaaaaa gcttttgaaa cagatatggc
    33721 caatcaagtg caaaaggtcg aaaaggaaaa agtggtcaag gttcgagaag acaagaaacg
    33781 tatgacctac caagaaaagc aggagtgggc aagtattgaa ggtgatattg aaaccttgga
    33841 aaaacgtatc gctgctattg aagaggaaat gcaggctaac ggctctgact ttggtaagct
    33901 ggctactctc caaaaagaat tggatgagaa aaatgaagca ctccttgaaa aatacgaacg
    33961 ctatgagtat ctcagtgaat ttgatagtta agactattcg aaaatcaaag tgcaaactat
    34021 gtcggcttca cattgccgac tcaagtacag cctgtgaccg gcttcctaat ttgcactttg
    34081 atttttattg agtataaaag aatagaaaaa tcccgtctca tgaaaggatt tctctattct
    34141 tttttggttt cttgatgaaa gatattttgc caggtttcgt ggcgacgcca gatactggta
    34201 tggatgatac catctaactc atagcagatg agtttggttt tttgactgat ggaagtaatt
    34261 tgaatgtcct tgatagcaga atttaactct tttttggtct tataagcctc tttatccatc
    34321 tgctctccat cttggcgaat atagacaaaa tcatcagtca agagttcttc tagttggttt
    34381 ccttggtcaa tcaattgttc acgcataagc aattttttat agacattgtc taaaatttca
    34441 tcctcagaga taggtgctgg ttcgatatgg acatcggtat caaagacgcc aaaacgctcc
    34501 tccagcatag actcgacctg atccgcgatt tcatggcttt caaaaacaga caagtcagga
    34561 ttcatctcta gtgtaatatc caggtagatg ttgctaccgt aggtgcgacc tctttgcgat
    34621 ttgaccttgc tgattttggg aatttccatg atagcctttt ggtagtcctc gagcaggcgg
    34681 tcgtcaaagc catctgaaag actaaaggaa gactcgatga agatatcata ggcagtcttc
    34741 aagataaaga aagtgatgat gatagcaacc agtttatcca caatcggata attgaaacta
    34801 ctagctagga tggcaatggc ggttccaagt gaggtaacag cgtcagaaag attgtcctta
    34861 gcagctgcct tcagcgcctt ggagttggat ttcttactga ggcgagtatt gtagagatag
    34921 accacaaaca taatcgctgc agaaatgatt cctagagttg caccaagagg atcaatgacc
    34981 gtttcttccc gactgagaat cttttgaatg gtatctctta gaacatcgaa accgacatag
    35041 aacatgatga tagaagtgat caagcttgcc aaatcttcaa tcttccaatg accaaaacgg
    35101 tggtcacggt ctgcaggctg gcgcgccatc cgaatcccga ttaagagggc cacatttcca
    35161 atgatgtccg atacgttatt aaaaccatcg gccaccaaac tggatgaatg aaggagatga
    35221 ccagctgcta atttggctgc agacaagatc aaataggtcg aaatactgat aatggctcca
    35281 cgctcagcta acttgagatt tgagatagat tgcttcattc aacttccttt ctagtcacat
    35341 agcattctac cattatactg gttttcaagg aaaaattcaa ataaggcagt ttttactctt
    35401 tgaaaactcc ccaaaatttg gtataatggt actactcaag ggagtagctg gcagaaacct
    35461 gtgatagtgt cgtcattccg aattttatgc tgaaaagtat gctttccggc cctatcttaa
    35521 acagcgagac ttgttatgat taacaggtct ctttttgttt gtcgtgaaaa gatatgacag
    35581 aaagagagtc tgttttgcac acaatgtcaa ggaggagaca catgtcaaaa gaacaaaaac
    35641 gccaagcgtt ttatactcag agccctgaag aggtcttgca ggctgtggat gcgaccgagc
    35701 aaggtttgtc atcaagtgag gcggaaaagc gccttgccga atttgggcac aatgaactcg
    35761 aagaaggcga gaaacgatca atcttggtca aattcatcga gcaatttaag gatttgatga
    35821 ttatcatcct agttgcggca gcaatcttgt cagtcgtgac ttctggtggg gaagatatcg
    35881 cagatgccat tatcatccta gctgtggtta tcatcaacgc tgcctttggt gtttaccaag
    35941 aaggaaaagc tgaagaagct attgaagccc tcaaatccat gtctagtcca gttgcccgcg
    36001 ttcttcgtga tggacacatg gcagagattg actctaaaga attggtacca ggcgatatcg
    36061 ttgcccttga agcaggtgac gtggtaccag cggacctacg tttgatagaa gccaactctc
    36121 ttaaaattga agaagcagcc ttgacaggtg aatctgtacc agtcgaaaaa gacttgtcag
    36181 tcgagcttgc gacagatgct ggtattggtg accgtgtcaa catggccttc caaaactcaa
    36241 acgtaaccta tggtcgtggg atgggtgttg ttgtcaatac aggtatgtac actgaagttg
    36301 gtcatattgc tggtatgctt caagatgcgg atgagactga tacaccactc aaacaaaatt
    36361 tgaacaacct ttctaaggtc ttgacctatg ctatcttggt cattgccctt gttacttttg
    36421 tagtgggtgt cttcattcaa gggaaaaatc cacttggtga gttgttgact tctgttgccc
    36481 ttgccgttgc agccattcca gaaggacttc ctgctatcgt taccatcgtt ctttctcttg
    36541 gtactcaagt tttggccaaa cgacattcca tcgttcgtaa gttgccagca gttgaaacac
    36601 ttggttcaac tgaaatcatc gcttctgata agactggtac gctgactatg aacaagatga
    36661 cagtcgaaaa agtcttctac gatgcggttc tacatgactc agctgatgat attgaactag
    36721 gtcttgaaat gccactactt cgttcagttg tcttggccaa tgatacgaaa atcgatgtgg
    36781 aaggtaactt gattggtgac ccaaccgaaa cagcctttat ccaatatgcc ttggacaagg
    36841 gctatgatgt caaaggtttc ttagagaaat atcctcgtgt agctgaattg ccatttgact
    36901 ctgaccgtaa gctcatgtca acagttcacc cattgccaga tggtcgtttc cttgtagcag
    36961 tcaagggtgc gccagaccaa ctcttaaaac gttgtcttct tcgtgataag gctggggata
    37021 ttgctccgat tgatgagaag gttacaaatc tcattcatac aaacaattct gaaatggctc
    37081 atcaagcctt gcgtgtcctt gcaggtgctt ataagattat cgatagtatt ccagaaaatc
    37141 tcacttctga agagcttgaa aatgatttaa tttttactgg tttgattggg atgattgacc
    37201 ctgaacgtcc tgaagccgct gaggctgttc gtgtggctaa ggaagcggga atccgtccaa
    37261 ttatgattac aggtgaccac caagacacag cggaagccat tgccaaacgt ttgggaatca
    37321 ttgacgcaaa cgatacagaa ggtcacgttt taactggtgc tgaactcaat gaactgtcag
    37381 atgaagaatt tgaaaaagtc gttggtcaat actctgttta tgcccgtgtg tctccagaac
    37441 acaaggttcg tatcgtcaag gcttggcaaa aacaaggtaa agtcgttgcc atgacaggtg
    37501 acggtgtcaa tgacgcgcca gctctgaaaa cagccgatat cggtatcggt atgggaatca
    37561 ctggtacaga ggtttctaag ggggcttctg atatgattct tgcagatgat aactttgcga
    37621 ctattatcgt cgcagtggaa gaaggacgta aggtcttctc aaacattcaa aagactattc
    37681 agtacctact ttctgctaat actgctgaag tattaaccat cttcctatca accttgtttg
    37741 gttgggatgt cttacagccg gttcatcttt tgtggattaa cttggtaacg gataccttcc
    37801 cagctatcgc tcttggtgtt gaacctgcgg aacctggtgt catgaatcat aaaccacgtg
    37861 gacgcaaggc aagcttcttc tcaggtggtg ttttgagttc tatcatttat caaggtgtac
    37921 tccaagcagc tcttgttatg agtgtttatg gccttgcgat tgcttaccca gttcatgtgg
    37981 gtgacaatca tgctattcat gcagatgccc taacgatggc ctttgcaacc cttggtttga
    38041 ttcagctctt ccatgcctac aatgtcaagt ctgtctacca atccatcttg acagttggcc
    38101 cattcaagtc taagaccttt aactggtcca tcttggtatc ctttatcctt ctcatggcaa
    38161 caatcgttgt agaaccgctt gaaggaatct tccacgtaac caaactagac ttgtcacaat
    38221 ggggaattgt tatggctgga agcttctcaa tgattatcat cgtcgaaatc gttaagttta
    38281 ttcaacgcaa actcggtttt gacaagaatg cgatttaagg gaggtttcgg atgtcagcat
    38341 atcaattacc gaccgtatgg caggatgaag ctagtaatca aggagctttt acggggctaa
    38401 acagaccaac agcaggtgcc cgtttcgaac aaaacttgcc aaaaggagaa caagcttttc
    38461 agctttattc actgggaaca ccaaatggtg tgaaggttac tatcttattg gaagaattac
    38521 tagaagctgg ttttaaggaa gcggcttacg acttgtataa gattgctatc atggatgggg
    38581 atcaattcgg ctcagacttt gtgaagctca atccaaattc caagattcca gccttattgg
    38641 accagtcagg tactgaaaac gtaagagtct ttgagtctgc tcatattctt ctttaccttg
    38701 ctgagaaatt tggagccttt ttaccaagta atcctgtgga aaaggtagaa gttttgaatt
    38761 ggctattctg gcaagcaggt gcagcacctt ttctaggtgg gggatttgga catttcttca
    38821 attatgctcc tgaaaaattg gaatatccta ttaaccgttt tacgatggaa gtgaaacgcc
    38881 agttggattt attggataag gaattggctc agaaacctta tattgcaggc aatgactata
    38941 cgattgcaga tattgctatc tggtcttggt atggacagtt agttcaagga aatctttacc
    39001 aaggttctgc aaaattcttg gatgcctcaa gttatcaaaa tctagtaaaa tgggcagaaa
    39061 aaattgccaa tcgtccagct gttaagcgtg gcttggaagt aacttataca gaaattaaat
    39121 agcaaaaaag tcatcttcgg atggcttttt gctacagttg agggaaaggg tttgcagtta
    39181 tcggcttttg tgctataatt gctataatat agtaaaaagc aagaggagtg tgaggaagtg
    39241 gaaaagaaaa ttgtgaagga tatcttattt ttatctcaag tgtctcatcc ggcaagtcag
    39301 gaggaccttt atcttgccag agatttgcag gatacactct tagcaaatcg tgatacctgt
    39361 gttggtctag ctgccaatat gattgggatg cagaagcgcg tgattatctt taatcttggc
    39421 ttggttcccg tggtcatgtt taacccagtg cttctgtcct ttgaaggatc ctatgaggca
    39481 gaagaaggct gtttgtcctt ggtaggtgtg agatcaacta agcgttatga aaccataagg
    39541 cttgcctatc gtgacagcaa gtggcaggaa cagaccatta ccttgacagg cttcccagct
    39601 cagatttgcc agcatgagct ggatcacttg gaaggacgaa ttatttagga ggaaagcaaa
    39661 tgaaacgaat agtctttgaa cttattttta tcgcaacgac ctggtatatc tttttaccgc
    39721 cccttaacct gaccagctgg gaatttctct tcttcctctg tgggcatttg ttagttgtgg
    39781 caatcttatt tggctttggc aaggggataa accttgtcaa aacggttcat gtgcgccacg
    39841 gtaaggcgga agctgcctta aatcttgagg gtttcaaaat caatcggtta gggaaaattc
    39901 tgttagcttc gattggagga attcttctct tggcagcttt ggtttccttg gtaacttcta
    39961 gcatgtttca ggctaaaaat tatgccaatg tagtcacggt tacggaaaaa gactttactg
    40021 aatttcctaa gagtgacacc agtaaggttc ctatcctaga tagaagtact gctgaaaaaa
    40081 ttggagaccg ctacttgggt tccctaaccg ataaggtgtc gcaatacgta gcggcagata
    40141 cctataccca attgacaatt gatgggaaac cttatcgggt cacaccacta gaatatgcag
    40201 accctatcaa atggtttaac aatcaagcca agggaatcgg tgagtatatc aaggtggaca
    40261 tggtaactgg aaatgcggat ttggtggact tgaagacacc aatcaagtat tcagactcgg
    40321 agtattttaa ccgtgatgtc aaacgtcacc tgcgcttgaa gtacccgacc aaaatcttta
    40381 aaactccatc ttttgaggtg gacgatgagg gcaatccttt ctatgtagca acggtttacc
    40441 aaaagcaatt tggacttgct gttcctcgtc ctgcttcagt cattatcttg gatgctacaa
    40501 atggagaaac taaggaatac agcttatcag atgttccaga atgggtggac aggatctatc
    40561 cagcagagga aaccattgag caaatcaact acaacggcaa gtacaaggac ggtttcttga
    40621 atgccatgat ttccaagaaa aacgtgaccc agacgaccaa tggctataat tacttgtcta
    40681 tcggtaatga catctatctc tacacaggtg tgacgtcggc taatgcggat gagagtaatc
    40741 ttggtttcat ccttgaaaat atgcgaacag gagaaattac taagtatagc ttggcttctg
    40801 cgacagaaga atcagcccgt gaatcagcag aaggtgctgt tcaggagaaa tcctacaaag
    40861 caaccttccc aatcctcatc aacctcaatg acaagcctct ctacatcatg ggcttaaagg
    40921 acaatgctgg cttggtcaaa gagtacgccc tggtagacgc agtcgagtac caaaatgtta
    40981 tcgttgctac tacagtggaa gagatgctca gcaagtatgc caataaaaac gaccttgaaa
    41041 ttgacaatgc aacgacagaa agcatcaagg gagtagtagc agacctcaaa tcagctgtta
    41101 tcaagggaga cactgtctac ttctttaaag ttgatggcaa gatatacaag gtaaaggctt
    41161 cagtatccga tgaccttcct taccttgaaa atggtaaaac cttcgaaggt caagtaggaa
    41221 aagacaatta tctcaagacc tttaaagtac aataatactc ttcgaaaatc tctagctcta
    41281 tct
//
LOCUS       DAVBCG010000005        40912 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBCG010000005 DAVBCG010000000
VERSION     DAVBCG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.005"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..1537)
                     /locus_tag="VQ495_000210"
     CDS             complement(<1..1537)
                     /locus_tag="VQ495_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2530226.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLKTIQKHITTIEKELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSSISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIR"
     gene            complement(1665..2279)
                     /gene="lepB"
                     /locus_tag="VQ495_000211"
     CDS             complement(1665..2279)
                     /gene="lepB"
                     /locus_tag="VQ495_000211"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2530227.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            complement(2283..3164)
                     /locus_tag="VQ495_000212"
     CDS             complement(2283..3164)
                     /locus_tag="VQ495_000212"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2530228.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            3251..3553
                     /gene="zapA"
                     /locus_tag="VQ495_000213"
     CDS             3251..3553
                     /gene="zapA"
                     /locus_tag="VQ495_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2530229.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            3550..4098
                     /locus_tag="VQ495_000214"
     CDS             3550..4098
                     /locus_tag="VQ495_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2530230.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIFYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            4202..6538
                     /locus_tag="VQ495_000215"
     CDS             4202..6538
                     /locus_tag="VQ495_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2530231.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     regulatory      6702..6790
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            6881..8203
                     /locus_tag="VQ495_000216"
     CDS             6881..8203
                     /locus_tag="VQ495_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2530232.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     gene            8421..8969
                     /locus_tag="VQ495_000217"
     CDS             8421..8969
                     /locus_tag="VQ495_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2530233.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            9079..9978
                     /locus_tag="VQ495_000218"
     CDS             9079..9978
                     /locus_tag="VQ495_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2530234.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(10003..11277)
                     /gene="serS"
                     /locus_tag="VQ495_000219"
     CDS             complement(10003..11277)
                     /gene="serS"
                     /locus_tag="VQ495_000219"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2530235.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(11491..11862)
                     /locus_tag="VQ495_000220"
     CDS             complement(11491..11862)
                     /locus_tag="VQ495_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2530236.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(11865..13229)
                     /locus_tag="VQ495_000221"
     CDS             complement(11865..13229)
                     /locus_tag="VQ495_000221"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2530237.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13554..14339
                     /locus_tag="VQ495_000222"
     CDS             13554..14339
                     /locus_tag="VQ495_000222"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2530238.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            14433..14867
                     /locus_tag="VQ495_000223"
     CDS             14433..14867
                     /locus_tag="VQ495_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2530239.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            14867..15841
                     /locus_tag="VQ495_000224"
     CDS             14867..15841
                     /locus_tag="VQ495_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2530240.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            15901..16125
                     /locus_tag="VQ495_000225"
     CDS             15901..16125
                     /locus_tag="VQ495_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2530241.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            16244..17218
                     /gene="fabK"
                     /locus_tag="VQ495_000226"
     CDS             16244..17218
                     /gene="fabK"
                     /locus_tag="VQ495_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2530242.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            17211..18131
                     /gene="fabD"
                     /locus_tag="VQ495_000227"
     CDS             17211..18131
                     /gene="fabD"
                     /locus_tag="VQ495_000227"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2530243.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            18165..18896
                     /gene="fabG"
                     /locus_tag="VQ495_000228"
     CDS             18165..18896
                     /gene="fabG"
                     /locus_tag="VQ495_000228"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2530244.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            18918..20153
                     /gene="fabF"
                     /locus_tag="VQ495_000229"
     CDS             18918..20153
                     /gene="fabF"
                     /locus_tag="VQ495_000229"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2530245.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            20156..20641
                     /locus_tag="VQ495_000230"
     CDS             20156..20641
                     /locus_tag="VQ495_000230"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2530246.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            20638..21060
                     /gene="fabZ"
                     /locus_tag="VQ495_000231"
     CDS             20638..21060
                     /gene="fabZ"
                     /locus_tag="VQ495_000231"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2530247.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            21072..22439
                     /gene="accC"
                     /locus_tag="VQ495_000232"
     CDS             21072..22439
                     /gene="accC"
                     /locus_tag="VQ495_000232"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2530248.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            22476..23342
                     /locus_tag="VQ495_000233"
     CDS             22476..23342
                     /locus_tag="VQ495_000233"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2530249.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            23339..24106
                     /locus_tag="VQ495_000234"
     CDS             23339..24106
                     /locus_tag="VQ495_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2530250.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            24265..24450
                     /gene="briC"
                     /locus_tag="VQ495_000235"
     CDS             24265..24450
                     /gene="briC"
                     /locus_tag="VQ495_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2530251.1"
                     /translation="MTGTETFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            24791..25168
                     /locus_tag="VQ495_000236"
     CDS             24791..25168
                     /locus_tag="VQ495_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2530252.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(25217..25639)
                     /gene="nusB"
                     /locus_tag="VQ495_000237"
     CDS             complement(25217..25639)
                     /gene="nusB"
                     /locus_tag="VQ495_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2530253.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(25632..26021)
                     /locus_tag="VQ495_000238"
     CDS             complement(25632..26021)
                     /locus_tag="VQ495_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2530254.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(26043..26603)
                     /gene="efp"
                     /locus_tag="VQ495_000239"
     CDS             complement(26043..26603)
                     /gene="efp"
                     /locus_tag="VQ495_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2530255.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(26732..28174)
                     /gene="gatB"
                     /locus_tag="VQ495_000240"
     CDS             complement(26732..28174)
                     /gene="gatB"
                     /locus_tag="VQ495_000240"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2530256.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(28174..29640)
                     /gene="gatA"
                     /locus_tag="VQ495_000241"
     CDS             complement(28174..29640)
                     /gene="gatA"
                     /locus_tag="VQ495_000241"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2530257.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(29640..29942)
                     /gene="gatC"
                     /locus_tag="VQ495_000242"
     CDS             complement(29640..29942)
                     /gene="gatC"
                     /locus_tag="VQ495_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2530258.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            30155..31699
                     /locus_tag="VQ495_000243"
     CDS             30155..31699
                     /locus_tag="VQ495_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2530259.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            32011..32160
                     /locus_tag="VQ495_000244"
     CDS             32011..32160
                     /locus_tag="VQ495_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2530260.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            32271..32447
                     /locus_tag="VQ495_000245"
     CDS             32271..32447
                     /locus_tag="VQ495_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2530261.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            32563..32751
                     /locus_tag="VQ495_000246"
     CDS             32563..32751
                     /locus_tag="VQ495_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2530262.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            32908..33273
                     /locus_tag="VQ495_000247"
     CDS             32908..33273
                     /locus_tag="VQ495_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2530263.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            33276..34943
                     /locus_tag="VQ495_000248"
     CDS             33276..34943
                     /locus_tag="VQ495_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2530264.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            35143..36843
                     /locus_tag="VQ495_000249"
     CDS             35143..36843
                     /locus_tag="VQ495_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2530265.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDILPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            36836..37312
                     /gene="ilvN"
                     /locus_tag="VQ495_000250"
     CDS             36836..37312
                     /gene="ilvN"
                     /locus_tag="VQ495_000250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2530266.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            37378..38400
                     /gene="ilvC"
                     /locus_tag="VQ495_000251"
     CDS             37378..38400
                     /gene="ilvC"
                     /locus_tag="VQ495_000251"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2530267.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            38507..38743
                     /locus_tag="VQ495_000252"
     CDS             38507..38743
                     /locus_tag="VQ495_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530268.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            38981..39337
                     /locus_tag="VQ495_000253"
     CDS             38981..39337
                     /locus_tag="VQ495_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2530269.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            39387..40637
                     /gene="ilvA"
                     /locus_tag="VQ495_000254"
     CDS             39387..40637
                     /gene="ilvA"
                     /locus_tag="VQ495_000254"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2530270.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT        11798 a         7785 c         9424 g        11905 t
ORIGIN      
        1 gtcgaatctg actagctagg gtgacgatgg ttgattcttc gctttgtcga taaatttttt
       61 ccaagcgagt ctgaggaatc aaaggaatat gaagcagatc cgctagaacc tgtccaggac
      121 tgacagaagg tagctgatca ctgtcaccta cgatgaggat cttactgtta gaagagatac
      181 tggagaagag ttgattggcc agccaagtat ccaccataga gaattcatcc acgatgataa
      241 agtcagcatc taggtaatct tccagatgac tggtatcatc gtcacctgtc attcccaagt
      301 ggcggtgtat ggtcgcgcta ggcaaacctg tcaattcatt catgcgacga gcagctcgac
      361 cagttggagc agcaagaaga atgggcaggt tgcttttctt cctgaggtca agtccctcta
      421 acagggcgta aacagcgatg atcccattga taacagttgt cttaccagta ccaggtccac
      481 ctgtcaggat aaagacctta ttctggatag catcacagat agcctgtttt tgaatgttat
      541 catactcaat tccaagctct ttctcgatag tagtgatatg tttttgaatg gtttttaaat
      601 catgactctt ctgttttcct ttttcaagga tacgaaccaa gtgactgcgg atgccttcct
      661 cagcgaaaaa gaggctgttg tcaaagatct tggtatcaat ctgctgaact ttgtcttctt
      721 cgatcaggta ggagagctct tgggcaactt ggctggggtc tagttccacg ggacgggaag
      781 actcaaggag agtaagggtt tgttccagca aatcccgtgc ttcaacatag gtgtcccctg
      841 tttccataca ggcctgaaaa agactgtgaa ctagaccagc gcggaagcgt tcaggagcct
      901 gactttcgat gcctagttcc tcagctagtt ggtcagcaat ggtaaagccc aaacccttga
      961 tatcctcaac cagctggtag ggataatttt caaccacatc aagggtttct tccttgtaaa
     1021 agtcttgaat ctgaaaggct agtttgttgg gaatgccgta gttggctagt ttggccaaaa
     1081 ccatctccgt tccgtagttg agacggagag tggagacgaa agcctcgcga tttttggcag
     1141 agagtcctgc gatgccttct aacttttctg ggtgttgcaa aatttcgtca atggtatttt
     1201 cgccataggt atccacgatt ttctgagctg tcttgagacc aatccccttg aaatggctac
     1261 ttgaaaagta cttgaccaag cccttactag ttggttttgc gcgatcataa cgactgattt
     1321 gcagttgttc tccatacttg gagtgctgga caatttgccc ccaaaaagta tagtcttcgc
     1381 cctcaattac atcagccatg gttcctgtga caatgatttc aaaatcatca aaatcctctg
     1441 cgtccgtatc gtcgatttct aggaggagga tgcgataaaa attgctggga ttttcaaaaa
     1501 taatccgttc aatggttcct gaaaaataaa cttccataaa attcctttgc atgaataggt
     1561 gagagttgag gtgtttctgt tctggtaagt ttagatagtt accaatcatt ttctcacgat
     1621 agaagaagag gctgagattg gtgattctcg gcctcttagg tttcttaaaa tgttccgata
     1681 cgggtgattg gccataagcg gaatttagct tcccctgtga tatcttttgc tttgaaggta
     1741 cctacgtggc ggctgtcgct cgaaaccaag cggtcatctc cgaggagaag gtattctcct
     1801 tctggaacag taaagctaaa gttggtgttg tagttgacat caactgtgaa ggcttgagct
     1861 ttttgagcga tacttctaaa gaaagttcct ttatttcctt caaagccctt gcctgagtaa
     1921 gtgctttgga gtttgtcatc cttgaagcgt ttgatatagt ctgctagata aggctcgtcc
     1981 gtttctttgt cattgatgta gagtttatca ttttcgtaac gaatggtgtc gccaggcatt
     2041 ccaatcacgc gcttgacgat gtccttattc ccatcttcct catgggccac cacgatatca
     2101 aaacggtcaa taggaaggtg ttttacaacg aagagaattt cgccatccgc tagggtcgga
     2161 tccatggaat gtccttctac gcgaacattg ctccaaaaaa agatacgact taaagctagt
     2221 aatgacagaa ttaggaggaa caatccccac tcttttaaga aatttttaaa tgaattcata
     2281 acttaccttt ctaagcgttt tttcgctttt tcagtgtttt taaagtgcaa tttggcgcag
     2341 aagctgagtc cctgcatacc ataggcttgc aaaatctggc tagccacctt gtcagaagcc
     2401 gttccagctc cacttgggag ctgataaccc agttctcgtc ccaaattttc aagattttcc
     2461 agaaagagat cacgcgcaat gacagaagaa actgcgacag acaagtattt gccctcagcc
     2521 ttttcttcta agctgatagg attgctgaaa cgattggcct cttgtgccaa gtacttgtca
     2581 taatttttag cactggtaaa ggcatcaatc acaattttct caggctgaac acctttttga
     2641 aggaggagat agatagcctg attatggagg gcaaccttaa ccgaaacagc attgtatcgg
     2701 tctccgatga cctcgttgta cttgctgggt gagagaagga gtgcctggtg ctggattttt
     2761 tccttgagaa taggagcaat ctgacggatc ttttggtcgg tcagagtctt agaatccccc
     2821 acaccgagtt ttcgtaaaaa gtcgtgctgg tcagatgtga caaaggcagc cacaactgca
     2881 agcccaccaa agtaggaacc atttcccacc tcatctgtcc caattaaagg aagattttgc
     2941 cccctgtttt cctctacaag ttgatagcca aagaaactag cgtatttctc agccccttca
     3001 ccctgaagca agatttttcc agaagtatag atagaaaccg ttgcttgagg tagtttcaaa
     3061 aagtagcgga tatagggatt cttactggga gccagactgg tttgatagtg ttcaagaaaa
     3121 gcctgaatat ccttttcgct tggtgtgagt gtgatacttg ccatagtttc tattgtacca
     3181 caaaagcagt aaaatttgta aaaactgaca aaattagcga attttggtat aatatcgtga
     3241 ggtgaatttt atggcaaatc taaatcgatt caaatttaca ttcgggaaaa aatcgttaac
     3301 cttgacaagc gaacatgata acctttttat ggaggaaatc gctaaggttg cgacagaaaa
     3361 ataccaagca attaaagaac aaatgcctag cgcagatgat gaaacaatcg ctcttttgtt
     3421 ggcagtcaac tgtttatcaa ctcagctcag ccgtgagatt gaatttgacg ataaggagca
     3481 agagctagaa gaactccgtc acaagcttgt gacttgtaag caagaacaga gcaagattga
     3541 ggattcctta tgatttcatt ccttcttcta ttggtcttgg tttggggatt ttatatcggc
     3601 tatcggagag gcctgctctt acaggtttat tacctgattt cagccatggc atcggctttt
     3661 atggctggcc agttttataa ggggcttgga gagcaattcc atttattgct cccttatgca
     3721 aattcgcagg aaggtcaggg gactttcttt ttcccatcgg atcaactctt tcagctggat
     3781 aaggtctttt atgcaggtat cggctacttg cttgtatttg ggattttcta tagcattggt
     3841 cgtttgcttg gtcttctctt acacttgatt cctagcaaaa aactgggtgg taagttgttc
     3901 caagtttcag caggtatctt gtccatgttg gtgaccttat ttgtcttgca aatggccttg
     3961 acaatcttgg cgaccatccc catggcagtt atacaaaatc ctcttgaaaa gagtatcgtc
     4021 gcaaaacaca tcatccagag cataccgata acaaccagtt ggctcaaaca aatctgggtg
     4081 acaaatttaa tcggataaaa agggcaggag ttttcctagc cctttgttta cagatttgac
     4141 tcgaatctat cagaatgtaa aaagctacca cacctagaca ttcaaagaca aggaaataaa
     4201 gatgaataag aaaatattag aaacattaga gttcgataag gtcaaggcct tgtttgagcc
     4261 tcatttgttg accgagcagg gcttggagca attgagacag ctggctccga ctgccaaagc
     4321 agataaaatc aaacaggctt ttgctgagat gaaagaaatg caggctcttt tcgtcgagca
     4381 accgcatttt actattctct caactaagga aattgcagga gtctgcaaga ggttggagat
     4441 gggagcggat ctcaatatcg aggagttcct actcttgaaa cgcgtgcttc ttgccagccg
     4501 agaacttcaa agtttttatg ccaatctgga aaatgtcagc ttggaagaat tagccctttg
     4561 gtttgagaaa ttacatgatt ttccgcaatt acaaggaaat cttcaggcct ttaatgatgc
     4621 gggtttcatt gaaaattttg ccagtgaaga attggcgcga atccgtcgaa aaatacatga
     4681 tagcgagagt caggtacgcg atgttttaca agacttgctc aagcaaaaag cgcagatgtt
     4741 gacggaagga attgttgcta gcagaaatgg ccgtcaggtt ttaccagtca aaaacaccta
     4801 ccgcaataag attgcaggtg tcgttcatga tatttctgct agtggaaaca ccgtctatat
     4861 cgaaccacgt gaggtagtca aactgagcga agaaattgct agtctgcgag cagatgagcg
     4921 ctatgaaatg cttcgcattc tccaagaaat ttctgagcgt gtccgccctc atgcggctga
     4981 gattgctaat gacgcttgga ttatcggtca tctggacttg attcgtgcca aggttcgatt
     5041 tatccaagaa agacaagcag tcgtgcctca gctgtcagaa aatcaagaga ttcaactgct
     5101 ccatgtctgc catcctttgg tcaaaaatgc cgtcgcaaat gatgtccatt ttggtcaaga
     5161 tttaacagct attgtcatta caggtcccaa tacaggtggg aagaccatca tgctcaaaac
     5221 tctgggcttg acacaggtca tggcccagtc aggattgccg attttagcag acaagggaag
     5281 tcgtgttggt atttttgaag aaatctttgc tgatattgga gatgagcagt ctattgagca
     5341 gagcttgtct accttctcta gccatatgac caatatcgtg gatattcttg gcaaggtcaa
     5401 ccaacattca ctcttacttt tggatgagtt gggggctggt actgatcccc aagagggagc
     5461 agcccttgcc atggctattc tggaggacct tcgcctgcgt caaatcaaga ccatggcgac
     5521 gacccactat ccagaactca aggcctacgg tattgagaca gcctttgtgc aaaatgccag
     5581 tatggagttt gatactgcaa ctcttcgccc gacctatcgc tttatgcagg gtgttcctgg
     5641 ccgaagtaat gcctttgaaa ttgccaaacg tctaggccta tctgaagtta tcgtaggaga
     5701 tgccagtcag cagatcgatc aggacaatga cgtcaatcgt atcattgagc aattagaaga
     5761 gcagacgctg gaaagccgca aacgtttgga caatatccgt gaggtggagc aagaaaatct
     5821 caagatgaac cgtgcgctaa aaaaactcta caacgagctt aatcgtgaaa aggaaaccga
     5881 gcttaacaag gcgcgtgaac aggctgctga gattgtggat atggccctaa gtgaaagtga
     5941 ccagattctc aaaaatctcc acagtaaatc ccaactcaag ccccacgaaa tcattgaagc
     6001 caaggccaag ttgaaaaaat tggctcctga aaaagtggac ttgtctaaaa ataaggtcct
     6061 tcaaaaggcc aagaaaaaac gagctccaaa ggtgggagat gatatcgtgg ttctcagtta
     6121 tggtcagcgt ggtaccttga ccagtcaact caaggacggt cgctgggaag cccaagttgg
     6181 cttgattaag atgaccttgg aagagaaaga gtttgatctt gttcaagccc agcaagaaaa
     6241 accagtcaag aagaaacagg tcaatgttgt gaaacgaact tctgggcgag gacctcaagc
     6301 tagactggat cttcgaggca agcgctatga agaagccatg aatgagctag ataccttcat
     6361 cgaccaagcc ttgcttaaca atatggctca agttgatatc atccatggta tcggaacagg
     6421 agtcatccgt gaaggagtta ccaaatactt gcaaagaaac aaacatgtca agagtttcgg
     6481 ctatgcccca caaaatgctg gaggcagtgg tgcgactatt gtcactttta aaggatagca
     6541 gtattctgga ctttataaag taaaaactgt tgaactaatt tttactaata aacacattga
     6601 caaaagccaa cattttttgt aaaattagaa tcaattaaat accaacaccg aatgaagttt
     6661 aatagaagtg gggaatcgtt tgattttcca tgactgtaaa tggacggaac tctggagaga
     6721 ccgtaaaggc accgaagggg caaggcaggc aactgctcaa actctcaggt aaaaggacag
     6781 agctaggata gaccgctttt tagcatttat ctaagcattc cagagtacat gtatcttgca
     6841 tgtgctcttt cttttggggt tgaaacgata ggagaaggaa atgttagaat tgcttaaatc
     6901 aatcgatgct tttgcttggg gaccgcccct cttgatttta ttggtcggaa cagggattta
     6961 cctaaccatg cggctaggac tcttgcaggt tttgcgtctg cccaaggcct ttcagcttat
     7021 ttttatccag gataagggac atggtgatgt atccagtttt gcagctctgt gtacagcctt
     7081 ggcatcaact gttggaacag gaaatatcat aggagttgcg acggctatca aggttggtgg
     7141 accaggagct ctattttgga tgtggatggc ggctttcttt ggaatggcta ccaagtatgc
     7201 ggaaggactc ttggccatca aataccgcac caaggacgac catggtgcag tagcgggagg
     7261 tcccatgcac tatatccttc tagggatggg agaaaagtgg cgaccacttg ctgttttgtt
     7321 tgcagtagca ggagtattgg ttgctctctt gggaatcgga accttcaccc aagtcaactc
     7381 gattacagaa tctatccaaa atacaacgac gatttcgcca gccatcacag ctctcgtctt
     7441 gtctgtcttt gtagcgattg cagtctttgg tggactcaag tctatttcta aggtttcaac
     7501 tactgttgtt ccttttatgg ccctcattta tatcttagga actcttacag ttattttctt
     7561 taatatcgga aaaatccctg gcacaatcgc tttagtcttt acctcagctt ttagtcccct
     7621 tgctgcggta ggtggatttg ctggtgctag cgttcggatg gctattcaaa atggtgtggc
     7681 gcgtggtgtg ttctcaaacg aatctggtct gggttctgct cctattgcag ctgcagctgc
     7741 caagacaaat gaaccagtag agcaaggttt gatttccatg acaggaacct ttattgatac
     7801 cctcatcatt tgtactctaa ctggtttgac catcttggta actggagttt ggagtggtga
     7861 cttgaatggg gttgccttga ctcagtcagc tttctcaaca gtcttttcac actttgggcc
     7921 tgccctcttg accatcttcc ttgtactttt tgcctttaca acgattctag gttggaacta
     7981 ttacggagaa cgctgttttg agttcctctt tggggctcgc tttatctggc tctaccgtgt
     8041 gttttttgtg ctcatggtct tgttaggagg atttatcgag ttggatatgg tctggattat
     8101 cgcagatatc gttaacgcct tgatggctct gccaaacttg attgccctct tggtcttgtc
     8161 gccagtcgtt attgctgaaa ctaaaaagta ttttgacaaa taatggaatc acacagctcg
     8221 tgtgattttt ttgcctataa ataattttta tcagcctgat atataatata tattatgcaa
     8281 gtgattggaa gtgtgataaa ctaatataac aacgaaaatc ttatgagaga ataaagcttt
     8341 atcttagata aacttaggaa tcaggatttt ataagatttt ccaataatat tagtgtcaat
     8401 aataagaaaa gaggtatctt atgacaacat ttactatcca tacagtagag tcagcaccag
     8461 cagaagtgaa agaaattctt gaaacagtag aaaaagacaa caatggctat attcccaacc
     8521 taatcggtct cttggccaat gccccgactg ttttagaagc ctaccaaatt gtctcatcta
     8581 tccaccgtcg caacagcctg acacccgttg agcgtgaagt ggtgcaaatc acggcagccg
     8641 tgaccaatgg ttgtgccttc tgtgtcgcag gtcacacagc cttttccatc aaacaaatcc
     8701 agatgaatga tgacttgatt caagctcttc gcaatcgtac tccaattgaa acagatccta
     8761 aattggatac cctagctaag tttaccttgg cagttatcaa taccaagggt cgtgtaggag
     8821 atgaagcctt gtctgagttt ttagaagctg gctacactca acaaaatgcc ttggatgtgg
     8881 tttttggtgt cagcctagca atcctctgta actatgccaa caacttagct aatacaccaa
     8941 ttaatccaga attgcaacct tatgcctaat tcatatctga gtaaaatgaa gtctgaaata
     9001 agcggacttc gtttttttaa atcctcattt aagcgctttt atgttatact gaaactaaca
     9061 tgattattgg aggttaggat gaaaaaactc cccttagtat tttcaggttg tttgctaggt
     9121 ttggcaggag ctggaaatct tattttagat acgttgccgg ttctatccca tctttttagt
     9181 ctgactggtt tggttttatg gatttacttt ctaattctgc atctctttaa ttggaaagaa
     9241 accaagcaag aattgaccaa gccccctctt ttgtcaggaa tggcaacctt tcctatggct
     9301 gggatgattt tatcgaccta tgtctttcgc gtattctctt atcttccttt ggtagcacaa
     9361 gggatttggt ggttttcatt tctcttggat ttgaccttga ttgctggttt taccatcaag
     9421 tttgcttgtt cagggcggag ggttcatgcc actccaagtt ggacggttct ctatgtgggg
     9481 atagcagtgg ctgccttgac ctatcctctg gtaggtatta tcgaaattgc ctatgcgacc
     9541 ttgagttttg gttttctctt gaccttctat ctctatcccc ttatttatag cgatttaaag
     9601 aaacatccac tcccactagc cttgcttgga caagaaggaa tctactgtgc tcctttctct
     9661 ctactcttgg cttctctagt tcgagtagga ggaaccagcc tgccgacttg ggtcttgatt
     9721 gtcatgattt tggcttctca atccttcttt ttctttgttt taactcgtct gcccaacatt
     9781 ttaaaacaag gttttcaacc agccttctca gccctcacct tcccaaccat tatcacagcg
     9841 acctcgctca agatggctca gggaattttg aaacttccat ttctggatta cctggtattg
     9901 gctgaaacca ttatatgcct aactatttta ttctttgtac taggtgctta tctgatttgg
     9961 ttacgaaaaa aggtctagct agaaatagct aaaccttatt ttttatggtt tgatgacttc
    10021 agctccaccc atgtatggac gaagtgcttc tgggatggtc acagaaccat cttcattttg
    10081 gtaattttca agaattgcag ccactgtacg tccaactgca agtccagagc cgttcaaggt
    10141 atgaaggagt ttcaccttgc catctgcttc atcacggtaa cggatttggg cacgacgggc
    10201 ttggaaatct tctgtgtttg aacagcttga gatttcacgg taattgtttt gtgctggaat
    10261 ccacacttcc aagtcgtaag tcttcgcagc tgagaagccc atatctccag tagagagagc
    10321 aacgacacgg tatggaaggt tgagtttttg aagaatgttt tcagcgttgg ctgtcatttt
    10381 ttccaattct tcgtaagatt cttctggttt ggcaaatttg accatttcaa ccttgtggaa
    10441 ttggtgcaaa cggatcaagc cacgcgtatc acgaccggca gaaccagcct cagaacggaa
    10501 tgacggactc atggcagtga agtagattgg aagatctttg ccgtctaaga tttcatcacg
    10561 gtagtagttt gtcagaggaa cttcagcagt tggaatcaag acaaagttgg tatcgctgag
    10621 ttcaaaagta tcttccttaa attttggata ctgaccagta ccaaacatag aatcatggtt
    10681 gactatgtaa ggtgtgatga cttcagtata gccttctttt ccatgttcat ccaacataaa
    10741 gttgtagata gcacgttcca aacgagcacc gaggccttta tagaagagga agcgagcgcc
    10801 tgttacctta ccaccgcgtt cccagtcaag gataccaagg tcttcaccga gatcccagtg
    10861 agctttaggt tcgaagtcaa actcgcgtgg agtaccccaa cggcgaactt ccacattgtc
    10921 gtcttcgtca gtcccaacag gaacgctgtc agctgggata tttggaagag tcgttgtaaa
    10981 ttctgtcaat ttagcatcga tttctgccaa ttcagcatcc aaggctttaa cctcagcaga
    11041 tagattttgc atggcagcaa tcttgtcatc tgtattttcc ttgttgcgct tagcttgggc
    11101 aatctcagca gaaactgtgt tacgttctgc tttgagagtt tcaaccttga ccaagatgtt
    11161 acgacgttta gcatcgattt ctttcatttc attcaagaca gcagcatcta caccacgtgt
    11221 agctaatttt tctgcgacag cttcaaaatc tgtacgaata cgtttgatat ctaacataag
    11281 aactccttta tgaaaaaagc acgcctgaca aaacgttgga gtggcagggc cacggttcca
    11341 tccaacttca caggtgtgca cttgattgtg tatgtaattg ttactaacgg tagaatttca
    11401 cctatccccc ctatctgctc gcagtacccg cagactttct gaaagaagaa gataacctac
    11461 ttatccgttg ctatgattat actaaagttt ctactttttt gcaaatagat ttttaaattt
    11521 ttggctaatt gtctgaatca gggtcggaag tttgacgacc ttgtcattgc ctagtttttc
    11581 gcgtgcaatt ttgagaatgg cacctgagtc ttttgaagca aagaggaatt ttcctttgtc
    11641 tgtaaagact tcgaagtggc ggctgatttt gcgtccagtg acattggctc caatctgatt
    11701 gatatggctc caaggaatct ggataaattg ttcgacattg acatctgggt aaaattccaa
    11761 agcctgatct ccgacaagga atttcccaac tttcccagcg atagagaggt aggaagtgcc
    11821 tgtcgtactg aggagtactg ttttgttaag tgattgggcc atgcttagtc ttccttactt
    11881 tctccaaaaa aggcattgta gagggcttta attgctgctt tctcttggtc tttattgaca
    11941 acaaacataa tagaaacttc actagaacct tgagacatca tctggatgtt gattttgttt
    12001 tcagatagag cgcgtgtcgc agtagcagtc actccgatat ggctcttcat tttttcacca
    12061 acaatcataa tgatagaaag gtcgtgttcg atttctgcat gatctacttt agccttttga
    12121 accaactgac gcaggatttc ttcttccttg atgggagtta gttggcgaga acggagaatg
    12181 atagaaagat cgtcgatacc tgttggcata tgttcccaac cgatgttaag ttcttccagg
    12241 atttgcagaa ccttgcggcc aaatccaacc tcacggttca tgaggtattt cgacatgtta
    12301 atgctgacaa agcctgagtc accagcaatt cccacaactg gaaattcatc attactgtgt
    12361 tttagaacga tacgagtacc tggatggtca gggttgttgg tattcttgat aaccagagga
    12421 atttttccac ggtaggcagg aagaagagcc tcgtcatgaa ggactgagaa gcctgcatag
    12481 gccaactcgc gcatttcacg gtaggtcaac tcaggaatcg agtgtggttg gtggataata
    12541 ccagggtggg ctgcaaagat accatcaacg tccgtaaagt tttcatagag gtcagctttg
    12601 acaccagcag caatgataga acctgtaata tcagaacctc cacgtgagaa agtacagatt
    12661 tgattttcct tagtgacacc aaagaaacca ggaatgacaa ggacttcatt ggtgtttgtc
    12721 aattcttcaa tcttgtcata acttgatgga atgatgcgag cgtgaccagg ttcacttgtg
    12781 accacaatcc cagcttctct agggtgcata tagcgtgcat cgataccatt ttggttaaag
    12841 taggcagcaa tcaatttggc attgttattt tcaccggctg ctaggaaagt atcgtagaga
    12901 aattcatttt cttcaatagg aagagtggcc aaggcgtgaa tgcttttaga aattttttct
    12961 agcacagctg gttttagtcc caattcacta accatagcag catagcggtc gataatccag
    13021 ctttggctct tgctaatatc gttacccgca acatagtcgc ggtagtattt aatcagggca
    13081 tccgtaacct tagtatcttc agcattgcgt ttaccaggcg cagaaacgac tacaaaacga
    13141 cgctctgaat cgcttttgac gatgtttaaa actttttcta attgactagc agaggcaaga
    13201 gaactacctc caaatttaac aactttcata agaactccta aagtaagtat tttatacgat
    13261 tatagcagaa agaaaggctt ttttcaatga agaaaataag atgctttcta gcctaaagcg
    13321 agcctttctt atcggctgag acactttcag tcgatttttt cttgctttct gctcctaaaa
    13381 aagatatact ttgacagtga aataatttaa cactcaatga aaatcaaaga gcaaactaag
    13441 aagctagacg aagtcagctc aaaatactgt tttgaggttg cagatggaag ctgacgcggt
    13501 ttaaagagat tttcgaagag tataaactgc ttataaaata aaaaggagcc ctgatggaac
    13561 acattattta tcagcttgaa gaggacttgg caatccttac cttgaaccgt cctgaggtcg
    13621 caaatggttt tcatattccc atgtgtgagg agattttaga agctctgact ttggcagaag
    13681 aaaatccagc tgtgcatttt atcttaatca atgccaatgg aaaggtcttc tcagttgggg
    13741 gagatttggt agagatgaag cgggcagtgg atgaggatga tattccatca ttgacaaaaa
    13801 tcgcagagtt ggtcaatacg atttcttata aaatcaagca aatagccaaa cctgttttaa
    13861 tggaagttga cggtgctgtt gcaggtgccg cagcgaatat ggctgttgcg gcagatttct
    13921 gtttggcgac ggataaggct aagtttatcc aagcctttgt tggtgttggt ttggctccag
    13981 atgcaggggg aattcatctc ttgagccgca gtatcggtgt gacgcgtgct gctcaattag
    14041 ctatgacagg agaggcttta acggcagaaa aagccctaga atgggggttg gtttaccgcg
    14101 tttctgaagc tgaaaaactt gaaaagacga gagaacagct tcttaaaaaa ttaagacgtg
    14161 cttcaagtaa ttcctatgct gccattaaga agttggtttg ggagagccaa tttaaagact
    14221 ggcagggtta cgctacttta gaactgaacc tacagaaatc cttagctcaa acagaggatt
    14281 tcaaagaagg agtgcgagct cattcggaga gaagaagacc taaatttaca ggaaaataaa
    14341 aaatacttgc atcattcttt gaattttgat ataattcttt ctgtcaaatg ttttgattgt
    14401 aaaagttttt tgaagaagga gggatatgaa aaatggacta ccaacgaatc aatgaatatt
    14461 taacatctat atttaacaat gtccttgtaa ttgaggaagt gaacttgaga ggtagtcgtt
    14521 ttaaggatat ctccatcaaa gaaatgcata cgattgatgt catcggtaag gctccagatg
    14581 tgactccaag tcaagtgtca aaagagttga tggtaactct tggaactgtt acgacaagtt
    14641 tgaacaattt agagcgtaag ggctacattg agcgagttcg gtcagaacag gatcgtcgtg
    14701 tggtgcatct gcatttgaca aagaagggtc gcttgattca tagactgcat aaacgcttcc
    14761 acaaggccat ggtagaaaaa attattgatg gcatgagcga ggaagaaatt gctgtcatgg
    14821 gtaaaggttt gactaatctt taccaatttt tggaggattt gaaataatgg cttttgcaaa
    14881 aataagtcag gttgctcatt atgtgccaga gcaagtggtt acaaatcacg acttggctca
    14941 gattatggat accaatgatg agtggatttc aagtcgaacg ggaatacgac aaaggcatat
    15001 ttcaagaaca gaatctacca gtgatttggc tacagaggtt gctaagaaac tgatggcaaa
    15061 agctggaata acaggagaag aactggattt tatcatccta gctaccatta ctccagattc
    15121 gatgatgccc tctacagctg ctcgtgttca agctaatatt ggtgctaata aagcctttgc
    15181 ttttgactta accgcggctt gcagtggatt tgtatttgct ctttcaactg ctgaaaagtt
    15241 tatcgcttct ggtcgctttc aaaaaggctt ggtgattggt agtgaaaccc tctctaaggc
    15301 agtcgattgg tcggatcgat caacagctgt gttgtttgga gatggtgctg gtggtgtctt
    15361 gttagaagct agcgagcaag agcatttctt agctgagagt ctcaatagcg atggaagtcg
    15421 cagcgagtgt ttaacttatg ggcattcagg tttgcattct ccattttcag atcaagaaag
    15481 tgcagattca tttttgaaga tggatggacg cacagtcttt gattttgcca ttcgagatgt
    15541 agccaagtct atcaagcaga ctattgatga atctcctata gaggtgacag acttggatta
    15601 tctgctactt catcaagcca ataaccgtat tttggataag atggctagaa aaattggtgt
    15661 tgaccgagcc aaacttccag ctaatatgat ggaatatggc aataccagtg cagccagtat
    15721 cccgatttta ctttcagagt gtgtagaaca aggtctcatc cctttagatg gtagccaaac
    15781 tgttcttcta tcaggcttcg gtggaggctt gacctggggc acgctcattc ttacaattta
    15841 ggtaatcatg tggtgaacac attgttataa aaaaactatt tattttaaag gagtcctatc
    15901 atggcagtat ttgaaaaagt acaagaaatt atcgttgaag aacttggaaa agacgcatca
    15961 gaagtaacac ttgaatcaac ttttgatgat ttggacgcag attcattgga cttgttccaa
    16021 gtaatctcag aaatcgaaga tgcttttgat atccaaatcg aagcagaaaa tgacttgaaa
    16081 acagttggtg acttggttgc ttacgttgaa gagcaagcaa aataagcaga atattagtag
    16141 aaggagtagg gagaaaccca ctccctcttg tttaggtaat agtttgactg tcaaattatg
    16201 gtgaaatcga actattacgt aagcaaggaa ggttggaggc actatgaaaa cgcgtattac
    16261 agaattattg aagattgact atcctatttt ccaaggaggg atggcctggg ttgctgatgg
    16321 tgatttggca ggggctgttt ccaaggctgg aggattagga attatcggtg ggggaaatgc
    16381 cccgaaagaa gttgtcaagg ccaatattga taaaatcaaa tcattgactg ataaaccctt
    16441 tggggtcaac atcatgctct tatctccctt tgtggaagat atcgtggatc tcgttattga
    16501 agaaggtgtt aaagttgtca caacaggagc aggaaatcca agcaagtata tggaacgttt
    16561 ccatgaagct gggataatcg ttattcctgt tgttcctagt gtcgctttag ctaaacgcat
    16621 ggaaaaaatc ggtgcagacg ctgttattgc agaaggaatg gaagctgggg ggcatatcgg
    16681 taaattaaca accatgacct tggtgcgaca ggtagccaca gctatatcta ttcctgttat
    16741 tgctgcagga ggaattgcgg atggtgaagg tgctgcggct ggctttatgc taggtgcaga
    16801 ggctgtacag gtggggacac ggtttgtagt tgcaaaagag tcgaatgccc atccaaacta
    16861 caaagagaaa attttaaaag caagggatat tgatactacg atttcagctc agcactttgg
    16921 tcatgctgtt cgtgctatta aaaatcagtt gactagagat tttgaactgg ctgaaaaaga
    16981 tgcctttaag caggaagatc ctgatttaga aatctttgaa caaatgggag caggtgccct
    17041 agccaaagca gttgttcacg gtgatgtgga gggtggctct gtcatggcag gtcaaatcgc
    17101 agggcttgtt tctaaagaag aaacagctga agaaatccta aaagatttgt attacggagc
    17161 cgctaagaaa attcaagaag aagcctctcg ctgggcagga gttgtaagaa atgactaaaa
    17221 cagccttttt atttgctggt caaggtgccc agtatctagg gatgggacgg gatttctatg
    17281 atcagtatcc gattgttaaa gaaacgattg atcgagcgag tcaggtgctc ggttatgatt
    17341 tacgttatct catcgatacg gaagaagaca aactcaatca gacccgctat acgcaaccag
    17401 ccattctagc gacttcggtt gctatctacc gtttattgca agaaaagggc tatcagcctg
    17461 atatggttgc tggtttgtct cttggagaat actctgcctt ggtggcaagc ggcgccttgg
    17521 attttgaaga tgcggttgcc ttggtagcta agcgtggagc ctatatggaa gaagcggctc
    17581 ctgctgactc tggcaagatg gtagcagttc tcaatacgcc agtagaggtc attgaagaag
    17641 cctgtcaaaa agcttctgaa cttggagtgg ttactccagc caactataac acacctgcac
    17701 aaatcgtcat tgctggagaa gtggttgcag ttgatcgagc ggttgaactt ttgcaagaag
    17761 caggtgccaa acgcttgatt cctcttaagg tgtcaggtcc ctttcacacc gctctccttg
    17821 aacctgctag ccagaaacta gctgaaactc tagctcaggt aagtttttca gattttactt
    17881 gtcccctagt cggcaataca gaagctgctg tgatgcaaaa agaggacatt gctcagctct
    17941 tgacgcgtca ggtcaaggaa cccgttcgtt tctatgaaag tattggggtc atgcaagaag
    18001 caggcataag caactttatc gagattggac cggggaaagt cttgtcaggt tttgttaaaa
    18061 aaattgatca aactgctcac ttagctcatg tggaagatca agcgagttta gtagcacttt
    18121 tagaaaaata gactaaaata agtagaagtt ttgaaaggaa aaaaatgaaa ctagaacata
    18181 aaaatatctt tattacaggt tcgagtcgtg gaattggtct tgccatcgcc cacaagtttg
    18241 ctcaagcagg agccaacatt gtcttaaaca gtcgtggggc aatctcagaa gaattgctcg
    18301 ctgagttttc aaactatggt atcaaggtgg ttcccatttc aggagatgta tcagattttg
    18361 cagacgctaa gcgtatgatt gatcaagcta ttgcagaact gggttcagta gatgttttgg
    18421 tcaacaatgc agggattacc caagatactc ttatgctcaa gatgacagaa gcagattttg
    18481 aaaaagtgct caaggtcaat ctgactggtg cctttaatat gacacaatca gtcttgaaac
    18541 cgatgatgaa agccagagaa ggtgctatca ttaatatgtc tagtgttgtt ggtttgatgg
    18601 ggaatattgg tcaagctaac tatgctgctt ctaaggctgg cttgattggc tttaccaagt
    18661 ctgtggcacg cgaggtcgct agtcggaata tacgagtcaa tgtgattgct ccaggaatga
    18721 ttgagtctga tatgacagct atcttatcag ataagattaa ggaagctaca ctagctcaga
    18781 ttccgatgaa agaatttggg caggcagagc aggttgcaga tttgacagta tttttagcag
    18841 gccaagatta tctaactggt caagtgattg ccattgatgg tggcttaagt atgtagcgaa
    18901 agctagaggt gaaaagaatg aaactaaatc gagtagtggt aacaggttat ggagtaacat
    18961 ctccaatcgg aaatacacca gaagaatttt ggaatagttt agcaactggg aaaatcggca
    19021 ttggtggcat tacaaaattt gatcatagtg actttgatgt gcataatgcg gcagaaatcc
    19081 aagattttcc gttcgataaa tactttgtaa aaaaagatac caaccgtttt gataactatt
    19141 ctttatatgc cttgtatgca gcccaagagg ctgtaaatca tgccaatctt gatgtagagg
    19201 ctcttaatag ggatcgtttt ggtgttatcg ttgcatctgg tattggtgga atcaaggaaa
    19261 ttgaagatca ggtacttcgc cttcatgaaa aaggacccaa acgtgtcaaa ccaatgactc
    19321 ttccaaaagc tttaccaaat atggcttctg ggaatgtagc catgcgtttt ggtgcaaacg
    19381 gtgtttgtaa atctatcaat actgcctgct cttcatcaaa tgatgcgatt ggggatgcct
    19441 tccgctccat taagtttggt ttccaagatg tgatgttggt gggaggaaca gaagcttcta
    19501 tcacaccttt tgccatcgct ggtttccaag ccttaacagc tctctctact acagaggatc
    19561 caactcgtgc ttcgatccca tttgataagg atcgcaatgg gtttgttatg ggtgaaggtt
    19621 cagggatgtt ggttctagaa agtcttgaac acgctgaaaa acgtggagct actatcctgg
    19681 ctgaagtggt tggttacgga aatacttgtg atgcctacca catgacttct ccacatccag
    19741 aaggtcaggg agctatcaag gccatcaaac tagccttgga agaagctgag atttctccag
    19801 agcaagtagc ctatgtcaat gctcacggaa cgtcaactcc tgccaatgaa aaaggagaaa
    19861 gtggtgctat cgtagctgtt cttggtaagg aagtacctgt atcatcaacc aagtctttta
    19921 caggacattt gctgggggct gcgggtgcag tagaagctat cgtcaccatc gaagctatgc
    19981 gtcataactt tgtaccaatg acagctggga caagtgaagt atcagattat atcgaagcta
    20041 atgtcgttta tggacaaggc ttggagaaag aaattccata cgctatttca aatacttttg
    20101 gttttggagg ccacaatgca gttcttgctt tcaaacgttg ggagaataga taagtatgaa
    20161 tttaaacgat attaaagact tgatgactca atttgaccag tcaagtttga gagaattttc
    20221 ttataaaaat gggacggatg agttgcagtt tagcaagaat gaagcgagac ctgtgcctga
    20281 agttgcaact caagtcgctc cagcacccgt tctagcaaca ccgagtccag tagctcctac
    20341 atctgctcca gcagagactg tagcagaaga agttccagct ccagctgaag caagtgtggc
    20401 tagtgaggga aatcttgtag agagtccact tgttggagtg gtttacttgg ctgctggacc
    20461 agataaacct gccttcgtta cagttggtga tagtgtcaaa aaaggtcaaa cattggtaat
    20521 tatcgaagcc atgaaagtca tgaatgaaat cccagctcct aaggatggtg tggtaacgga
    20581 aattctcgtc tctaacgaag aaatggttga gtttggtaaa ggattggtac gtatcaaatg
    20641 atcgatattc aaggaatcaa agaagccctt ccccaccgtt atcctatgct tctagtggac
    20701 cgtgtcttgg aggtgagcga ggataccatt gttgctatca aaaatgtgac catcaacgag
    20761 cctttcttta acggccactt tcctcaatac ccagttatgc caggtgttct gattatggaa
    20821 gccttggcgc aaactgctgg tgtgttggag ttatcaaaac ctgaaaataa aggaaaactg
    20881 gtcttttacg ctggtatgga taaggttaag ttcaagaagc aagttgtacc aggcgaccaa
    20941 ttggttatga cagcgacttt tgtaaaacgt cgtggcacca tagctgtggt tgaagcaaag
    21001 gctgaagtgg atggcaagct tgcagccagt ggtaccctta cttttgcaat tgggaactaa
    21061 ggaggttctc catgtttcga aaaattttaa ttgccaatcg tggtgaaatt gcggttcgta
    21121 ttatccgtgc ggcacgtgaa ttggggattg cgacggtagc ggtttattca actgctgata
    21181 aggaagctct tcatacgctg ttggcagatg aagcagtttg tattggtcct ggcaaggcaa
    21241 cagagtctta tctcaatatt aatgcagttc tatcagctgc agtcttgact gaggcagaag
    21301 ctattcaccc tggttttgga tttctcagtg aaaattccaa atttgcgacc atgtgtgaag
    21361 aagtaggtat caagtttatc ggtccatctg gtcatgttat ggatatgatg ggggataaga
    21421 tcaatgcgcg tgctcagatg attaaagcag gtgtgcctgt tataccaggt tcagatggag
    21481 aagtgcataa ctctgaagaa gctttgattg ttgctgaaaa aattggctat cctgttatgc
    21541 tcaaggcttc agcaggtgga ggtggtaaag ggattcgtaa ggttgaaaaa ccagatgacc
    21601 tcgtttctgc ctttgaaact gcctctagtg aggccaaggc caattatggc aatggtgcca
    21661 tgtacataga acgggttatc tatccagctc ggcacattga ggttcaaatc ctaggtgatg
    21721 agcatggaca tgtgattcac ttgggtgaac gggattgttc tcttcaaagg aataaccaaa
    21781 aggttttgga agaaagtccc tcgattgcaa tcggaaaaac gctgcgtcat gaaataggtg
    21841 ctgctgctgt tcgagcggca gagtttgttg gctatgagaa tgcaggaacc attgaatttc
    21901 ttcttgatga agcaagtagc aatttctatt tcatggagat gaatactcgt gttcaggtag
    21961 aacatccagt aacagagttt gtttcaggtg ttgatatcgt taaggaacag atttgcattg
    22021 cggcaggtca gcctttgtct gttaagcaag aagatattgt cctacgcggt catgccatcg
    22081 agtgtcgtat caatgcagaa aacccagcct ttaactttgc tccaagtcca ggtaagatta
    22141 ctaatctcta tctgccaagt ggtggagttg gcttgcgcgt ggattcagca gtttatccag
    22201 gttataccat tccgccttat tatgatagta tgattgccaa aatcatagta cacggcgaaa
    22261 atcgttttga cgccttgatg aaaatgcaac gtgccctcta tgaattagag attgaaggag
    22321 tgcagaccaa tgcagatttc cagcttgatc tcatttcaga tcgcaatgtc attgctgggg
    22381 attatgatac ttccttcttg atggaaacct tcttacctaa atatcaagaa aaagaataaa
    22441 atgactttaa gagtttaaac cgaaaagggg aatcaatggc tctatttagt aaaaaagata
    22501 agtatattcg aatcaatccc aatcgttcgg ttagggaaaa acctcaagct aagccagagg
    22561 ttccagatga attattttcc cagtgtccag gctgtaagca taccatctat cagaaggatc
    22621 tgggaagtga acgtatctgt ccgcactgta gctatacctt tcgtatttct gcccaagaac
    22681 gcttggcttt gacgattgat atgggaacct tcaaagaatt gtttacaggg attgaaagca
    22741 aggatccctt gcatttccct ggttaccaaa agaaactggc atctatgcgt gaaaaaacag
    22801 gtctgcatga agccgttgtg acaggaactg ctcttattaa aggtcagact gtggctcttg
    22861 ggattatgga ttctaatttt atcatggctt ctatgggtac ggttgtaggt gaaaaaatca
    22921 ctcgtttgtt tgagtatgcg actgtcgaaa aattaccagt tgtcctattc acagcctctg
    22981 gtggagcccg tatgcaggaa ggaatcatga gtctcatgca gatggctaag atctctgcag
    23041 cggttaaacg ccattcaaat gctggtctct tttacctgac cattttgaca gatccaacga
    23101 ctggtggtgt gacagcttct ttcgctatgg aaggcgatat cattctggct gaaccacaga
    23161 gcttggttgg ttttgctggg cgtcgtgtga ttgaaaatac ggttcgtgaa agcttgcctg
    23221 aggatttcca aaaggcagaa ttcctattag aacatggctt tgtggatgct attgtcaaaa
    23281 gaagagactt accagatacg attgctagcc tagtcagatt gcatggaggg agtcctagat
    23341 gaatattgca aaaatagtca gagaagcgcg tgagcagagt cgcttgacaa ccttggactt
    23401 tgcgacaggc atttttgatg aatttatcca attacatggt gaccgttctt ttcgtgatga
    23461 tggtgcagtt gttggtggta ttggttggct tggagaccaa gctgtaacag tggttggtat
    23521 ccaaaaaggc aagagtttgc aagacaacct caaacggaat tttggccaac cacatccaga
    23581 aggctaccga aaggcactgc ggttgatgaa acaggctgag aaatttggcc gtccagttgt
    23641 gacctttatc aatacagcag gtgcttatcc tggtgtcgga gcggaagaac gtggtcaagg
    23701 ggaagctatc gctcgcaatc tcatggaaat gagtgacctg aaagttccta ttatcgccat
    23761 tattatcggt gaaggtggtt caggcggggc tctggctcta gctgtcgcgg accgtgtctg
    23821 gatgctggaa aattctatct atgccattct cagtccagaa ggctttgctt ccattttatg
    23881 gaaggacggt actcgcgcca tggaagcagc agaactgatg aaaatcactt cgcatgaact
    23941 gttagaaatg gacgtggtgg ataaggtgat ttctgaagta ggactttcta gtaaagaact
    24001 aattaagagt gtcaaaaaag aactccaaac ggagctggct agactttcac aaaaaccgct
    24061 agaagagttg ctggaagaac gctatcaacg atttagaaaa tactaagaat gactaaatga
    24121 gactagaaag gtctcatttt ttatggaaaa atgcaattca ggtcgaaaaa ttgccagttg
    24181 aggagataga ctataaaata gacaaaaaaa gaaatatact ataaatgtga gcctacaaca
    24241 atctatttta agaaggtact tgcaatgaca ggtacagaaa catttacagt tctttcaact
    24301 gaggacttgg agcaaacttc aggtggtctt gctgtttggg aagatggata tagtagatgg
    24361 ttatattata gagaatttgc tccctatatg aggcaagggg cacttaattc ttatatagat
    24421 gcttggaagt acggcttccg aacagggtaa tctttcaaaa aagtacgatg agaaacacaa
    24481 agaaactaag acaatttgga atatttttgc ttattatttt actatctacc tatttaccac
    24541 aaacaattgg attgtatgtg acgatcattt tagggttagg agctgatgtc tattctctta
    24601 ttttaacagt gggattagtg ggaagtttcc ttttctatta atttggaggt taaaaaagaa
    24661 aaagagctgg gactggtcat ttgtcttctc cttatttgca acttatgtgg tttatcagat
    24721 tctcggtaat ttttaggcac gctatgctca tttgattaac cataggaata ttcatgatga
    24781 gtattttacc gtggttcttt caaatggaca accaaccttt ttatcgacta tcttatcttt
    24841 tgtcctgctt gtaatcatcg gtcctgtttt tgaggagact cttgatagag ggtattttat
    24901 gaataccttc tttcctaagt caaagtacta tttagatgtc atcttatcag gccttatctt
    24961 tgggattagc catttgatac tatctcacag agatccaatt agtttattat attatagtct
    25021 gattggtttc ttttttgccc ttgtttatcg ttctaccgac aatctgagat taacgattct
    25081 ctgccatagt ttttttaact ttctcaatca tgcaaaacct atctggattt ttgtttacaa
    25141 ttatatctat tatcattttt ttagatagtg ggaggcaaag aaaaagtgtt tgatatctat
    25201 caaacacttt ttctttttat tgttcttctg ttacaaactg gctgagcagt ccattgataa
    25261 aacgggcaga tttttgatcg gagaagtcct ttgcaagctc gatagcttca ttaacagcaa
    25321 ccagctgagg agtgtcaaat gaagtgattt caaaaactcc caagcgaagg aggtttctct
    25381 ccacgagcgt taagcgttca atggtccaac ctacttttaa atgctgagtg atttgcttat
    25441 ctagttcttc ctttttagct tgaacaccag aaacgaggtc tatcaaaaag gctggaagtt
    25501 gtacatccgt atcttcacga tcatgagtat aggcgaaacg acaagcagtt tcgacattcg
    25561 taccgaactc aaggctcatg agagcttgaa aagcgcattt acggagttgg cgtctagatt
    25621 ctaatagtgg actagtcatt gaggaagtcc tcgtcaaata gatctttcaa ttctggtttt
    25681 ggtgttttat ctgggacgat acctgcaacg tgaatattga tagcagcgag ttctacatca
    25741 gccatattac ggacggcatc tttgacagct ttctggatag caaccgctac cttaggaact
    25801 tttactccgt actcaaggta gagatagata tctgctgtga gttcttcgtc cacgttttta
    25861 agataaacgc cacggccgag tgaaagtttt gaaagggtat cagacactga tctgtttgaa
    25921 aaagagtgaa caccctctac ctttgcagta gcgatagcaa tgattttttc aagtacacgt
    25981 ggggcgataa cgatttcgcc aagttgttct tcaattccca tagaatgacc tctttctaga
    26041 gattaggcac gagaaacgta agttccttct gcagtgttga taacgagttt ttgtcctgct
    26101 tcgatgaagt ctggaacgtt tacgacaagt ccagtttcca tcgttgctgg tttaccagaa
    26161 cctgtaacag tagcaccttt gatagatggt tgagtttcag caactgtcaa ctcaacagta
    26221 gtaggaacgg tgacaccgat cacttcagtt ccgtagaatt ggattttcac atcagagttt
    26281 tcaaggatgt aaagcaattc gttttcaaca ttgactacag ggatttcgta ctggtcgtaa
    26341 gtttctgtat tcatgaagta tgctgtgtca tccattttgt acaagtattg agctgggaca
    26401 gtctcgataa tagcttgttc aaatttttcc tctggacggt agcttgtgtc aaatgtagaa
    26461 ccagtacgga catcacgcaa tttcatacgc atgatcgtgt ttcctttacc tggcttgtgg
    26521 tgactagctt ccaaaacgcg aatcaatttg ccgtcagctg tttcaaaggt cataccagct
    26581 tttaatttac ttgcttcaat catgttttta cctctttaat aaatattatt actcttcatt
    26641 ttaccataaa atactctgga aataaagcca aaatagttat tgggagttgg tagagaagaa
    26701 aaaaccaacc ttagggctgg ttttgttctg tctagttttc tttcaacttc gccaattcct
    26761 gtgcaagtag tttaagggca acttgtgggt tggcttggcc tttggttgcc ttcataagga
    26821 atcctgtaaa ggctttgtcg gcgttacgtt tgcctgactt gaagtcggca acagcagctt
    26881 cgttatcggc aaagacttgg tggatgattg ggatcaagat agctggatct gaaatttgaa
    26941 ccatacctgc tttttccacg tattcacgcg cgccaccgcc atttttagct agatggacaa
    27001 agactttctt ggcaatctta gatgaaatag taccgtcttc gatgatggca atcatttcaa
    27061 ccaagttttc tggtgtcaat tcgatttgtt ccagtgtttt accttcagca ttcaagaact
    27121 gagcgacttc cccttggagc cagttagaga cttgtttggc atcaccacct agggcaacag
    27181 ctttttcaaa gaagtcagaa gtgactttat tagcagtcaa ctgactagca tcgtagtctg
    27241 ataaaccaag gtcagataca taacgcgcac gacgttcttt tggaaactct ggcaactcag
    27301 tccgcatttc ctcaatccac tcgtcagaaa tttcaaagag gggtaggtct ggttctggga
    27361 agtagcggta gtcagcagcc ccttccttga cacgcatgag gatggttgct ttattcgctt
    27421 catcgtaacg gcgtgtttct tggcggattt gaccacctga gcgaagaatt tcagcctggc
    27481 gttggacttc gtattcaaga cctttacgaa cgtttgagaa ggagttgagg ttcttcaatt
    27541 cagtcttggt accgaatttc tcttgaccat aaggacgaag ggagatgttg gcatccacac
    27601 gcatagaacc ttcttccatc ttaacgtcag aaatgccagc gtactggata acttccttga
    27661 gggctgtcag ataagcatag gcctcctctg gcgaacgcat atcggcttca gatacaatct
    27721 caatcaaagg taccccttgg cggttaaggt caacgtaaga gtagccgtct gtaccgtggg
    27781 tgtttttacc agcgtcttcc tctaagtggg cgcgttcgat accaattttc ttggtcgtac
    27841 cgtcttctag ctcgacttca atccagccgt tataaccgat tggctcatca aactgagaaa
    27901 tttggtaggc tttgggatta tcagggtaga agtagttctt gcggtcaaag tgcatttttt
    27961 tgtggaggtc catgttgagg gcaagagcag ccttgatacc ggcatcaaca acccctttat
    28021 tgagaactgg tagaactcct gggaaagacc agtcaatcac gttagtgttg gcattttggt
    28081 catttccaaa gtgggcagaa gtaggtgaga agatttttga attggtgttg agctctacgt
    28141 ggacttcaag tccgatgact gtttcaaagt tcattagttg tcacctccaa aaatcacggg
    28201 ttgttgtttg tggtagtctg ttgttgcttc aaaagcagca gcagcttggt aaatggtttc
    28261 ctcagagtac ttgggaccaa tcaattggag tccgacaggt agaccttgag agaatccagc
    28321 aggaatcgaa attccaggca gtcctgccaa gtttacaggt atggtcaaga ggtcggctaa
    28381 gtacatggca actgggtcat ggttgagaga atccaagtca taggcaacac ttggagcagt
    28441 tggacccaaa atcaaatcgt aatccgcgaa gactttttcg aaatcttgaa tgataagtgt
    28501 acggacctgt ccagccttct tgtagtaggc atcgtagtaa cctgatgaaa gactgaaagt
    28561 acccagcatg atacggcgct tcacttcttc accaaaacct tggctacggc tgtttacata
    28621 gatttcatca agattagtcg catcttctgc gcggtagccg taacggatac cgtcaaaacg
    28681 ttgcaagttt gatgaagctt ctgatgaagc gatgatgtaa taaacggcaa caccgtattt
    28741 agagtgagga aggctgactt cttcgacgat agcacccaat ttttcaaagt gtttggccgc
    28801 gtttaagatt gtttccttaa cctctggatc aattccttcg cctaggtatt ccttaggcaa
    28861 agcaattttc atgcccttaa tgtcttggcc gatttttgaa gtaaagtcgg cgatgcggac
    28921 aggagcagaa gtagagtctt tagtatcttc gctggcaata gcgttgagca agagggcatt
    28981 ttccttaaca gtaggagcaa aaggtccaat ctggtctaat gagctaccaa aggcaatgag
    29041 accgaaacgt gaaactgttc cgtaggttgg tttgagacca acgattccgt tgaaggcagc
    29101 aggttggcgg atggaaccac cagtatcaga accaagtgac aagcgaactt gtcctgaggc
    29161 tacagctgcg gcagaaccac ttgatgaccc accaggaacc ttgctgtggt cccaagcgtt
    29221 tttagttgct ccgtagtgtg aagtttcacc ggaaccaccc atagcaaatt cgtccatgtt
    29281 ggtctttcca acgacaatca tgcctttggt ttttgcattg gcaacagctg tcgcatcaaa
    29341 gattggctca tagttgtaga gcatttttga ggcagcagtt gtgagaatac cgtctgtaga
    29401 gatgttatcc ttaacagcaa gtggaattcc tgaaaggaca ttgttagcat caattccagc
    29461 ttcatcaatg gctttagctt gaacaagagc ttgctcctca gcgatggtga caaatgaatt
    29521 gagggcttcc tcacgagact tgatattttc aagtgttgct tgggtcaatt ctgttgcaga
    29581 aatttcctta gagacaagga gattgtgcaa ctcttcaata gttttattgt taaaagtcat
    29641 taggcatctc ctccatcgtc taggatagct ggcaccttga tatagtagtt gtcttgttca
    29701 ggtacgtttt taaacaagcg atcacggtct gttccttctt cggccacatc agggcggagt
    29761 acagtcttgc ggtcagccat agtcgtagta ggtgcgacac cagttgtgtc aacttcgccc
    29821 agcaattcaa ccatgtcaac aatcttagac aaggtggtcg caaaggcagc agtttcttct
    29881 tcagagaatc ttaattttga aagattggca acgtgtgtta cctcttcttg cgtaattttc
    29941 atcttgcatc ctttcgtgaa atgatgattt ttagtctgtt ctattttacc atattttcct
    30001 ataaataagg gagggggaag tgaaaagaaa tagaaattga aaaaaatgct aaaatccgat
    30061 ataatggaat gttgaaagga atggtaaaat ccaatgtaaa aatcattctc agctattgaa
    30121 acaagaaaaa tagagaatca aagaaagaga acttatgaat attcaagaag aaattaagaa
    30181 acgtcgtacc tttgccatta tctcccaccc ggacgcgggg aaaacaacca tcactgagca
    30241 gttactctac tttgggggtg agattcgtga ggctggtacg gtaaaaggga agaaaacagg
    30301 gacttttgct aaatctgact ggatggatat cgagaagcaa cgtgggattt ctgttacttc
    30361 atctgttatg caatttgact acgacggcaa gcgcgtgaat atcttagaca cgccagggca
    30421 cgaggacttc tcagaagata cctatcgtac cttgatggcg gtggatgctg cggtcatggt
    30481 cgtggactct gccaagggga tcgaggctca aaccaagaaa ttgtttgagg ttgtgaaaca
    30541 tcgtggcatt ccagttttta cctttatgaa caagctggac cgtgacggtc gtgagccttt
    30601 ggatctcttg caagaattgg aagaaatctt gggcattgct agctacccta tgaactggcc
    30661 tatcgggatg gggaaagcct ttgagggctt gtatgacctc tataaccaac gtttagagct
    30721 ttacaaaggg gatgagcgtt ttgccagtct agaagacggg gacaaactct ttggtagcaa
    30781 tcctttctac gagcaagtca aggatgacat tgagctttta aatgaagctg ggaatgagtt
    30841 ttcagaggaa gctattctgg ctggagaatt gacgcctgtc tttttcggtt cagccctgac
    30901 aaactttggt gtgcagacct tccttgaaat cttcctcaag tttgctccag aaccacatgg
    30961 tcacaagaaa acagacggtg aaattgtgga tccttatgac aaggatttct caggctttgt
    31021 ctttaaaatc caagccaaca tggatcctcg tcaccgtgac cgtattgcct ttgtccgtat
    31081 cgtatcaggc gaatttgagc gtggcatgag tgtcaatctc cctcgtactg gtaagggggc
    31141 caaactatct aatgttaccc agtttatggc ggaaagtcgt gagaatgtga tcaatgccgt
    31201 ggcaggtgat attatcgggg tttacgatac cggtacttat caagttgggg ataccttgac
    31261 agttggtaaa aacaagtttg aatttgaacc actgccaacc tttactcctg aaattttcat
    31321 gaaagtttct gctaagaatg ttatgaagca aaaatccttc cacaagggga ttgagcaatt
    31381 ggtgcaagaa ggagccgttc agctttataa gaattaccaa acaggtgagt acatgctagg
    31441 cgctgtaggt caactccagt ttgaagtctt taaacaccgc atggaaggcg agtacaatgc
    31501 agaagtagtt atgaacccaa tgggtaaaaa gaccgttcgt tggatcaagc ctgaggactt
    31561 ggatgaacgg atgtcgtcaa gtcgcaatat cttagccaaa gaccgttttg accaaccagt
    31621 ctttctcttt gaaaatgact ttgccctccg ctggtttgct gacaagtatc cagacgtaga
    31681 gttagaggag aagatgtgac tcagtaccaa caattggaac taaagttcca aattgttgga
    31741 cgctagtcgc tatttggcga actagctaac tgcctcacta actacgtttg gcaaatgaaa
    31801 tcgatttgta aaactccgtg tcgtaatgta aaaattgtaa accgtaatgg gacttcatct
    31861 aaaatctcca ctggtatggt tttacgaacg aataggcgaa aataatggtt acgtagaaac
    31921 atatgatggg aataaaaaga aatgagataa aaagtgaaag aagagaaaaa agagaaaaaa
    31981 gcgattgtct taggtgcaga taatgcttat atggataaag ttgaaacaac aataaaatct
    32041 ctttgtgttc atcattataa tcttaagttt tatgttttta atgatgacct tccgcgagaa
    32101 tggttccaat tgatggaaaa aagactagag actttgaata gtgagattgt gaatgtttag
    32161 attgacagta atattctgaa aggttatcgt ctcccctttg agggactttc atatgcggct
    32221 tttttaggta ttttattcca aagtatgtaa gcgagtctcg tgtactgtat ttggattcag
    32281 atattgttgt aagaaaatcg atagatgagc tgtgggattt ggacttgacg gctataccct
    32341 tagctgcagt tagagatgat ttttatacac ataattttaa ttctggtgtt ctgttaatca
    32401 acgatggcat gtggagagca gaaaatgtca cgcaagacct gatttaagac acaaataaaa
    32461 aatcttccaa ttcccttgca aaaaacaaga aagtgtgata acatagtacg gtatgtggtg
    32521 ctagcacatc cgctatatta gatctaatag gaggaaaaca caatggctaa agtatgttac
    32581 tttacaggtc gtaagactgt atcaggaaac aaccgttcac acgcgatgaa ccaaacaaaa
    32641 cgtgccgtaa aaccaaacct tcaaaaagtt actgttctta tcgatggtaa acctaaaaaa
    32701 gtttgggctt cagctcgtgc tttgaaatca ggtaaagttg aacgcgttta ataataaaag
    32761 caaaggagac cttagggtct tttttctttt attgtgggta taacatcgtt tgaaaaatag
    32821 agtaaatatc cgctgataca tcattctgct tttacacttg ggctgaaata tgataaaata
    32881 gagtatcaac tagttgaggt aaaaaggatg actgtaaaaa ttaatacaaa agatggtcaa
    32941 atcgaactaa cagatgaagt gattgcaacc gtcgtaggtg gagcagcaac tgagattttt
    33001 ggtgtggtcg gtatggctag taaaaatgcc ctcaaagata atttccaagc cctgctaggt
    33061 aaggaaaatt attctaaagg tgtcgtcgta aaggcagccg aagatggcag tattgcagtt
    33121 gatgtatata ccgtgttaag ctacggaaca aagattagtg aagtgtcaaa aaacattcaa
    33181 gagcgtgttg cctttagctt ggaaaaccag cttggaatta ctgctcagac tgtaaatgtc
    33241 tacattcaaa atatcaaagt tgtaggagaa taaccgtgtc aaaaattact actagcttat
    33301 ttcaagaaat ggtgcaggct gcatcaactc gcttgaataa gcaagctgaa tatgtcaatt
    33361 cattaaacgt ctttccagtt ccagatggag atactgggac aaatatggga atgaccattg
    33421 aaaatggtgc taaagaagtt gcagacaagc cagcttctac agttggagaa gtagcgagca
    33481 ttcttgccaa agggcttttg atgggtgcgc gtgggaactc aggagtgatt acgtctcagc
    33541 ttttccgtgg attttcccaa gctatcaagg ataaagacga gttaacaggt caagacttgg
    33601 ctctggcctt ccaatcaggt gtggaagttg cctataaggc agtgatgaaa cctgttgaag
    33661 gaacgatttt gacagtttct cgtggagctg ctatcggtgc taagaaaaaa gctgagcaaa
    33721 cagatgacac tgttgaagtc atgcgcgcag ccttggaagg tgctaaaaca gctctagcta
    33781 aaacaccaga catgcttcca gtattgaaag aagttggcgt tgtggactca ggtggtcaag
    33841 gattggtctt catctacgaa ggtttccttt cagcccttac tggcgaatat attgcatctg
    33901 aggactttgt agcgactcct gccaacatga gtgagatgat caatgtagag catcataagt
    33961 ctgtagctgg tcacgtagca actgaggaca tcacgtttgg ttactgtact gaaatcatgg
    34021 tagctcttaa gcaaggtcca acctatgcta aagattttga ctacgatgaa ttccgtaact
    34081 acttggatga gcttggggat tctctcctcg ttgttaacga tgatgaaatt gtcaaagtcc
    34141 atgttcatac agaagatcca ggacttgtta tgcaagaagg tctcaaatat ggtagcttgg
    34201 tcaaggtaaa agttgacaat atgcgtaacc aacacgaagc acaggttgag aaagaagcta
    34261 ctcaagttat caagtcggct gaagaaaaag agtatgcttt gattgctgtg gtggctggta
    34321 aaggtctagc agatatcttc tgttctcaag gcgtggatta tgttatcgaa ggcggtcaaa
    34381 ccatgaaccc ttcaacagaa gactttatca aggctgttga acaggtcaat gcccgtaaca
    34441 tcatcttctt gccaaacaac aagaacatct tcatggcagc tcaatctgcg gcagaagttt
    34501 tggagcaacc agcagtagtg gtagaggctc gcactcttcc tcaaggtatg acaagtcttc
    34561 ttgcctttga tccaagcaag tccattgaag aaaaccaaga gcgtatgaca gctgctctta
    34621 gcgatgtcgt tagcggaagc gtcacaacag ccgtgcgtga tacaacgatc gatggcttag
    34681 aaatccatga aaacgataat ctaggtatgg tggatggaaa aattcttgtg tcaaaccctg
    34741 atatgcacca aacattgact gaaaccttga aacatatgtt ggatgaagat agtgaaatcg
    34801 taaccttcta tgtcggtgaa gacggaagcg aagaacttgc caatgaaatc gctcaagaaa
    34861 tcgtagaaga attcgaagac gttgaagtcg agattcacca aggtcaacaa cctgtttacc
    34921 catacctatt tagtgtggaa taagattaat gaaggactga gaaatcagtt cttttttctt
    34981 tttagtaaat gaaatcggta tctttttaat aaaaacaaaa ataacattca taaataaacg
    35041 ttaaaataga aaattcagaa aatttcttct tttatcttga aaaattttga aaaaatggta
    35101 tgatagtaac aagttatttt taagaggaaa gaaaggggaa taatggagaa aatcagttta
    35161 gaatctccta agacggggtc ggacctagtt ttggaaacac ttcgtgattt aggagttgat
    35221 accatctttg gttatcctgg tggtgcggtt ttgccttttt atgatgcgat atataatttt
    35281 aaaggcattc gccacattct agggcgccat gagcaaggtt gtttgcatga agctgaaggt
    35341 tatgccaaat caactggaaa gttgggtgtt gccgtcgtca ctagtggacc aggagcaaca
    35401 aatgccatta cagggattgc ggatgccatg agcgatagcg ttcccctttt ggtctttaca
    35461 ggtcaggtgg cgcgagcagg gattgggaag gatgcctttc aggaggcaga catcgtggga
    35521 attaccatgc caatcactaa gtacaattac caagttcgtg agacagctga tattccgcgt
    35581 atcattacgg aagctgtcca tatcgcaact acaggccgtc cagggccagt tgtaattgac
    35641 ctaccaaaag acatatctgc tttagaaaca gacttcattt attcaccaga agtgaattta
    35701 ccaagttatc agccgactct tgagccgaat gatatgcaaa tcaagaaaat cttgaagcaa
    35761 ttgtccaagg ctaaaaagcc agtcttgtta gctggtggtg gaattagtta tgctgaggct
    35821 gctacggaac taaatgaatt tgcagaacgc tatcaaattc cagtggtaac cagtcttttg
    35881 ggacaaggaa cgattgcaac gagtcaccca ctctttcttg gaatgggagg catgcacggg
    35941 tcattcgcag caaatattgc catgacggaa gcggacttta tgattagtat tggttctcgt
    36001 ttcgatgacc gtttgacggg gaatcctaag actttcgcta agaatgctaa ggttgcccac
    36061 attgatattg acccagctga gattggcaag attatcagtg cagacattcc tgtagttgga
    36121 gatgctaaga aggccttgca aatgttgcta gcagaaccaa cagttcacaa caatactgaa
    36181 aagtggattg agaaagtcac taaagacaag aatcgtgttc gttcttatga caagaaagag
    36241 cgtgtggttc aaccgcaagc agttattgaa cgaattggtg aattgacgaa tggagatgcc
    36301 attgtggtaa cagacgttgg tcaacaccaa atgtggacag ctcagtatta tccctaccaa
    36361 aatgaacgtc agttagtgac ttcaggtggt ttgggaacaa tgggctttgg aattccagca
    36421 gcaatcggtg ctaaaattgc taacccagat aaggaagtag tcttgtttgt tggggatggt
    36481 ggtttccaaa tgactaacca ggagttggct attttgaata tttacaaggt gccaatcaag
    36541 gtggttatgc tgaacaatca ttcactcgga atggttcgcc agtggcagga atccttctat
    36601 gaaggcagaa catcagagtc ggtctttgat atccttcctg atttccaatt gatggcgcag
    36661 gcttatggta ttaaaaacta taagtttgac aatcctgaga ccttggctca agaccttgaa
    36721 gtcatcactg aggatgttcc tatgctaatt gaggtagata tttctcgtaa ggaacaggtg
    36781 ttaccaatgg taccggctgg taagagtaat catgagatgt tgggggtgca gttccatgcg
    36841 tagaatgtta acagcaaaac tacaaaatcg ttcaggagtc ctcaatcgct ttacaggtgt
    36901 cctatctcgt cgtcaggtta atattgaaag catctctgtt ggagcaacag aagatccgaa
    36961 tgtatcgcgt atcactatta ttattgatgt tgcttctcat gatgaagtgg agcaaatcat
    37021 caaacagctc aatcgtcaga ttgatgtgat tcgcattcga gatattacag acaagcctca
    37081 tttggagcgc gaggtgattt tggttaagat gtcagcgcca gctgagaaga gagctgagat
    37141 tttagcgatt attcaacctt tccgtgcaac agtggtagac gtagcgccaa gctcgattac
    37201 cattcagatg acgggaaatg cagaaaagag cgaagcccta ttgagagtca ttcgcccata
    37261 cggtattcgc aatattgctc gaacgggtgc aactggattt acccgcgatt aaaaatccaa
    37321 cttaaatttg ttaaaccagc ctaaaaggca ataaataata gaaaagagag aaaagctatg
    37381 acagttcaaa tggaatatga aaaagatgtt aaagtagcag cacttgacgg taaaaaaatc
    37441 gccgttatcg gttatggttc acaagggcat gcgcatgctc aaaacttgcg tgattcaggt
    37501 cgtgacgtta ttatcggtgt acgtccaggt aaatcttttg ataaagcaaa agaagatgga
    37561 tttgatactt acacagtagc agaagctact aagttggctg atgttatcat gatcttggcg
    37621 ccagacgaaa ttcaacaaga attgtacgaa gcagaaatcg ctccaaactt ggaagctgga
    37681 aacgcagttg gatttgccca tggtttcaac atccactttg aatttatcaa agttcctgcg
    37741 gatgtagatg tcttcatgtg tgctcctaaa ggaccaggac acttggtacg tcgtacttac
    37801 gaagaaggat ttggtgttcc agctctttat gcagtatacc aagatgcaac aggaaatgct
    37861 aaaaacattg ctatggactg gtgtaaaggt gttggagcgg ctcgtgtagg tcttcttgaa
    37921 acaacttaca aagaagaaac tgaagaagat ttgtttggtg aacaagctgt actttgtggt
    37981 ggtttgactg cccttatcga agcaggtttc gaagtcttga cagaagcagg ttacgctcca
    38041 gaattggctt actttgaagt tcttcacgaa atgaaattga tcgttgactt gatctacgaa
    38101 ggtggattca agaaaatgcg tcaatctatt tcaaacactg ctgaatacgg tgactatgta
    38161 tcaggtccac gtgtaatcac tgaacaagtt aaagaaaata tgaaggctgt cttggcagac
    38221 atccaaaatg gtaaatttgc aaatgacttt gtaaatgact ataaagctgg acgtccaaaa
    38281 ttgactgctt accgtgaaca agcagctaac cttgaaattg aaaaagttgg tgcagaattg
    38341 cgtaaagcaa tgccattcgt tggtaaaaac gacgatgatg cattcaaaat ctataactaa
    38401 ttagaaatat atagcgctgg agatgatttt atgaaaaaga ttatgagaaa aattgcatcg
    38461 ttattattgg ttctagttgt ataatgtaat tacaccgtcg gtaatagtgc tagcagacca
    38521 aaataaagca gattggtcgt atgatgaaaa tgctgtaatt aacatttatg atgatgctaa
    38581 ttttgaagat ggtaggttgc atatgacctt tgaacaattc ttcaaattgg cacaaatagc
    38641 tagagaagaa ggtcttgaaa ttcattctcc gtttgagaga gctggtgcga ctaaatctgc
    38701 tcgttatata gcgaaatgga ttttgagaaa taaaaaatat taacaaatat agttggtaaa
    38761 tcattaggac ctaaatcagc tgttagattc ggagaagctt tatcctatat tgaaggtcct
    38821 cttcgcagaa taaatgagac gatagatggc ggtttatatc aaatagagca aattattgca
    38881 tctggattga aagaatcggg tttaaatgac tggactgcga aaactttagc ttcagctatt
    38941 cgtgggatat tagatgtact tatttagggg ttgaaatcat atgaatatta ccaatttgtt
    39001 ttctatcaag acaggatgtg atgaaactga taggcaactg caaaaactat tttttcagtt
    39061 ggatttacaa ttgggagaat tgacagatca actaagaaaa ttagattcta attttgttcc
    39121 tcgtagtcaa tttgtagaca cgttggattt gaatgatgta gaatataaag aaattttaaa
    39181 ctattttatc ttccatcgta atgatagtga agaaagtttg gtagaatggt tatatgattg
    39241 gatttccaca aatcgttatg aacttcctaa agagttttcg attcgtatgg ctcataaata
    39301 ccatgaaagt gttactgaaa ttttcggaga tgaataacta aaaaacagtc attagtgact
    39361 gttttttata gaaaaagagg ttttatatgt taagttcaaa agatataatc aaggctcaca
    39421 aggtcttgaa cggtgtggtt gtgaatactc cactggatta cgatcattat ttatcggaga
    39481 agtatggtgc taagatttat ttgaaaaaag aaaatgccca gcgtgttcgc tcctttaaaa
    39541 ttcgtggtgc ctattatgcc atttcccagc tcagcaagga agaacgtgaa cgtggggtag
    39601 tttgcgcttc tgcgggaaat catgcgcagg gagtagccta tacttgtaat gaaatgaaaa
    39661 ttcctgctac tatctttatg cctattacta cgccacaaca aaagattggt caggttcgct
    39721 tttttggtgg ggattttgta actattaaac tagttggaga tacctttgat gcctcagcca
    39781 aagcagctca agaatttaca gtctctgaaa atcgtacctt tattgatcct tttgatgatg
    39841 ctcatgttca agcaggtcaa ggaacagttg cttatgagat tttagaagaa gctcgaaaag
    39901 aatcgattga ttttgatgct gtcttggttc ctgttggtgg tggcggtctc attgccgggg
    39961 tttctaccta tatcaaggaa acaagtccag agattgaggt tatcggagta gaggcgaatg
    40021 gagcgcgttc catgaaagct gcctttgagg ctggaggtcc agtcaaactc aaggaaattg
    40081 ataaatttgc ggatgggatt gctgtgcaaa aggtaggtca gttgacctat gaagcaactc
    40141 gtcaacatat tcaaactttg gtaggtgtcg atgagggatt gatttctgaa accttgattg
    40201 acctttactc taagcaaggg atagtcgcag aacctgctgg agcagctagt atcgcctctt
    40261 tagaggtttt agctgaatat attaagggga aaaccatttg ttgtatcatt tctggaggaa
    40321 ataatgatat caaccgtatg ccagaaatgg aagagcgtgc cttgatttat gatggtatca
    40381 aacattattt tgtggtcaat ttcccacaac gtccaggagc tttgcgtgag tttgtaaatg
    40441 atatcctggg gccaaatgat gatatcacac gttttgagta tatcaaacga gctagcaagg
    40501 gaacaggccc agtattgatt gggatcgctt tagcagataa gcatgattat gcaggtttga
    40561 ttcgtagaat ggaaggtttt gatccagctt atattaactt aaatggtaat gaaacgcttt
    40621 ataatatgct tgtctgagga ctaataaaaa aatatcatat tatttgtaca actgatgtat
    40681 atatgctata atgaagatga agaaagggga cgattcctat ggttttagaa aacttatcgt
    40741 gcaattctta cttctgttca ttatagcact tatgatgttt ggctcagatt tccatccttc
    40801 attttgattt cctatctatt gacaagcata gtcacactgt ctttaatact cttcgaaaat
    40861 ctcttcaaac cacgttagct ctatctgcaa cctcaaaaca gtgttttgag ca
//
LOCUS       DAVBCG010000006        35442 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBCG010000006 DAVBCG010000000
VERSION     DAVBCG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.006"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(247..1110)
                     /locus_tag="VQ495_000255"
                     /pseudo
     CDS             complement(247..1110)
                     /locus_tag="VQ495_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1275..1469
                     /locus_tag="VQ495_000256"
     CDS             1275..1469
                     /locus_tag="VQ495_000256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530271.1"
                     /translation="MKKKRVSRMKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQ
                     LEIRVQSQKKSNGLDVGKAD"
     gene            1784..2080
                     /locus_tag="VQ495_000257"
     CDS             1784..2080
                     /locus_tag="VQ495_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2530272.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            2183..4198
                     /locus_tag="VQ495_000258"
     CDS             2183..4198
                     /locus_tag="VQ495_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2530273.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            4200..4832
                     /locus_tag="VQ495_000259"
     CDS             4200..4832
                     /locus_tag="VQ495_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530274.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            4973..5251
                     /locus_tag="VQ495_000260"
     CDS             4973..5251
                     /locus_tag="VQ495_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2530275.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(5760..6173)
                     /gene="ndk"
                     /locus_tag="VQ495_000261"
     CDS             complement(5760..6173)
                     /gene="ndk"
                     /locus_tag="VQ495_000261"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2530276.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(6284..9961)
                     /gene="rpoC"
                     /locus_tag="VQ495_000262"
     CDS             complement(6284..9961)
                     /gene="rpoC"
                     /locus_tag="VQ495_000262"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2530277.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(9994..13605)
                     /gene="rpoB"
                     /locus_tag="VQ495_000263"
     CDS             complement(9994..13605)
                     /gene="rpoB"
                     /locus_tag="VQ495_000263"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2530278.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLKEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            14021..14091
                     /locus_tag="VQ495_000264"
     tRNA            14021..14091
                     /locus_tag="VQ495_000264"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14053..14055,aa:Cys,seq:gca)
     gene            complement(14289..15620)
                     /locus_tag="VQ495_000265"
     CDS             complement(14289..15620)
                     /locus_tag="VQ495_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2530279.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(15911..16735)
                     /gene="endA"
                     /locus_tag="VQ495_000266"
     CDS             complement(15911..16735)
                     /gene="endA"
                     /locus_tag="VQ495_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2530280.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(16774..16962)
                     /locus_tag="VQ495_000267"
     CDS             complement(16774..16962)
                     /locus_tag="VQ495_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2530281.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(16955..18238)
                     /gene="murA"
                     /locus_tag="VQ495_000268"
     CDS             complement(16955..18238)
                     /gene="murA"
                     /locus_tag="VQ495_000268"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2530282.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(18262..18492)
                     /locus_tag="VQ495_000269"
     CDS             complement(18262..18492)
                     /locus_tag="VQ495_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2530283.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(18550..19587)
                     /locus_tag="VQ495_000270"
     CDS             complement(18550..19587)
                     /locus_tag="VQ495_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2530284.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(19571..20059)
                     /gene="coaD"
                     /locus_tag="VQ495_000271"
     CDS             complement(19571..20059)
                     /gene="coaD"
                     /locus_tag="VQ495_000271"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2530285.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(20049..20588)
                     /gene="rsmD"
                     /locus_tag="VQ495_000272"
     CDS             complement(20049..20588)
                     /gene="rsmD"
                     /locus_tag="VQ495_000272"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2530286.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(20653..21645)
                     /locus_tag="VQ495_000273"
     CDS             complement(20653..21645)
                     /locus_tag="VQ495_000273"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2530287.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(21757..22440)
                     /locus_tag="VQ495_000274"
     CDS             complement(21757..22440)
                     /locus_tag="VQ495_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2530288.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(22496..23236)
                     /locus_tag="VQ495_000275"
     CDS             complement(22496..23236)
                     /locus_tag="VQ495_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2530289.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            23270..23548
                     /locus_tag="VQ495_000276"
     CDS             23270..23548
                     /locus_tag="VQ495_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2530290.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            23626..24552
                     /gene="yidC"
                     /locus_tag="VQ495_000277"
     CDS             23626..24552
                     /gene="yidC"
                     /locus_tag="VQ495_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2530291.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(24627..25421)
                     /gene="pflA"
                     /locus_tag="VQ495_000278"
     CDS             complement(24627..25421)
                     /gene="pflA"
                     /locus_tag="VQ495_000278"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2530292.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(25547..26797)
                     /locus_tag="VQ495_000279"
     CDS             complement(25547..26797)
                     /locus_tag="VQ495_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2530293.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(26864..27691)
                     /gene="purR"
                     /locus_tag="VQ495_000280"
     CDS             complement(26864..27691)
                     /gene="purR"
                     /locus_tag="VQ495_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2530294.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(27972..28913)
                     /locus_tag="VQ495_000281"
     CDS             complement(27972..28913)
                     /locus_tag="VQ495_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2530295.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(28903..30159)
                     /locus_tag="VQ495_000282"
     CDS             complement(28903..30159)
                     /locus_tag="VQ495_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2530296.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(30161..30823)
                     /locus_tag="VQ495_000283"
     CDS             complement(30161..30823)
                     /locus_tag="VQ495_000283"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2530297.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(30786..31442)
                     /locus_tag="VQ495_000284"
     CDS             complement(30786..31442)
                     /locus_tag="VQ495_000284"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2530298.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(31453..32331)
                     /gene="rsgA"
                     /locus_tag="VQ495_000285"
     CDS             complement(31453..32331)
                     /gene="rsgA"
                     /locus_tag="VQ495_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2530299.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(32924..33697)
                     /locus_tag="VQ495_000286"
     CDS             complement(32924..33697)
                     /locus_tag="VQ495_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2530300.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(34387..35259)
                     /gene="rsmA"
                     /locus_tag="VQ495_000287"
     CDS             complement(34387..35259)
                     /gene="rsmA"
                     /locus_tag="VQ495_000287"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2530301.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
BASE COUNT        10263 a         7843 c         6854 g        10482 t
ORIGIN      
        1 agctagccac aggttgctca aaacactgtt ttgagattgc agatagagct gacgtgattt
       61 gaagagattt tcgaagaata taaatttgaa atcatgaaaa tccgtcaaac gggtggttgt
      121 tttgtctcgc acctcacgga gcgagacgga ctcagagtca cataattata aggctgatag
      181 tattaatcta actatcagcc ttacaggtta tttaacgttt cagaaaaact ataatgtcaa
      241 gattaactaa acagtatcta attccttcaa ataattttct atcttcatca gcattaaagg
      301 attgttataa atcttgcata gctctcttgc ttctatataa taatttttga cttgttctct
      361 gtctagaaat ttggctccag catttcctac aagaataagt agaggagcca attggtagct
      421 tgtctgtctt tgtttacaga gttcgatagt ctcaagagct tcttggatgg cttcattata
      481 tttttccttt gatactaggt agtgagcgta attgtaacga actctgatgt agccaaataa
      541 aaactcttga tgctcaaaat tttttgtctg atataactcc atcaaatgag agtagtttac
      601 ctcatattct tgttcacgac ccactaagga atagaaatta gatagagtat tcaacgcctt
      661 taaataaatc aaagtatttg aggagacttt taataatata ttttccaatg aagaaattgc
      721 ttcacactta ctgtcatatt gacagaagtc aataatagct ttaatccatt caaggtaagt
      781 tcggtcttct agtgttagaa aagtacttcg ttcaatctct attctataaa tatattccaa
      841 attttcataa tttctatcat ctaataggcg agcagataat tgcttgaaat tagagaggtt
      901 agatttaatt tcaatttgtt cattaaaaaa ataatctaat ggcacttcga gtcgttgtga
      961 gagtttgaac aaaaggtctg cggagggaat gaaataccct ctctcaattt tactaatctg
     1021 gctctattca caaattcctt ctgcaagagt ttgttgggag agtctcaact cttttctttt
     1081 caatctgaat ttctctgcga gtgtattcat taaagataat aaaccttcct atttgcttaa
     1141 tttcattata aaatttttag ttcaaaatag caagttaaag gaataaataa ttctttgcgg
     1201 taataaacaa aaaaaagtca aaaattacat aaaaatgctt ggctaaataa aatatatttg
     1261 ttaaaataag gataatgaaa aagaaaaggg tttcacgaat gaagaaaaat cgtggaattc
     1321 aaaaattagc tatattagta ttactaggtg tttttatgtt tagtaacaca attccttacc
     1381 aacagtttat tcagaagaat agacaattgg agattcgagt ccaatcgcaa aagaagtcca
     1441 atggtcttga tgttgggaag gctgattgat tttatattac tttaaaattc aactatttag
     1501 agaaaggtat tctgatgaga gcttgtaaca ataccagata gatataagaa tggagatgtt
     1561 tgataaaatg ctttcatagc taaccaactg ttgaaaatta tgagttacaa aatctaaagt
     1621 atttaagatt ggtttataaa attaggtctc tgcctgtaga aaggaacgtg cagttagaaa
     1681 ggaggaaaaa ataggatact ataattcaga ctgagatttc taattaaaat atatagttgc
     1741 atttcgctca ttttcataat atttcattca gaggagtaat aatatgttaa aatttagaaa
     1801 agtagttgca tcatcccttg ccgttttggc atttagtttg gctcttcctg ttttggcagt
     1861 ttctcaccgt ggtggcgagt ggacctatgg aggccatcat gatccaaata attggggagc
     1921 attttcgaat tattatcatg gttcacagta tcactgggcc tatgttggaa gtaatggacg
     1981 taacaatcaa aaaacagttt atgcaggagc acgtagtgcg gcctatgcgt ttgttaatac
     2041 aaattttggg gagcaagtta catttgatgc gggatggtaa agaataattg aagaagattc
     2101 ttttatcaat agttcagtaa gcttgttagt ttatagaggg caggggtatt tctgccctct
     2161 gttttagaat aaggaatgca ctatgaaaaa attatttata ctcatatcca acctgcttgc
     2221 tagtttgttc tttgtttggg tatttaccat ttggactgat acatatgtct cctattatta
     2281 ccccaacgtt gttgtacatg attcttcccc tgaaacaact tttcagcatg ttgctacacg
     2341 cttggaaaaa ctagcagaag aaacagatag ctttattgcg attcaacacc aagatcctaa
     2401 ctcagaaggt actacagttt tttcttatac gacctttggg gacggaaagt tgcctgatgg
     2461 gcttcaggaa aaaaaactag aagatgctca aagtagtagt gttgaaacaa actattttgt
     2521 attcgatgga cacctagata ttcacttgct aagggaggag ctaagtcaac ttggcttgac
     2581 taacatgaac ctgacaattc catctaaact atctacgtta atggctatct ttagtaacgg
     2641 atttcagtta atcagtctat tgattttcat cttaaccttt atagcactga ctttaattag
     2701 tcaaattagc caattacggt cgtcgggcat tcgcctcatt tcaggagaga aacggtggtc
     2761 cattttcctt agaccagttg gtgaggattt gaaagggata gctgttggtt tcagtttagc
     2821 tggcgtgctc gccattctta tgcaaaaaat tctgtcgctt ccaacgcaat ccctaatgac
     2881 cataggagca ggcttgctca gttataatct catcttgttg tcgatctccc tgtttttcgc
     2941 tcaactcttc gcagttggga taaaaaagat acacctgatg cagattataa aagggcaagt
     3001 gcctgtcaga ggaatcatta gtttgatttt aatcggtcaa ctattagcga ttattattgt
     3061 aacgctggga atagggagta gcttaaagta ttcccaagcc tggcaacagc atcggattgg
     3121 acaagaggct tggagtcagg aaaggcaact gattacccta tcaatcagcc gtgaaggaac
     3181 gagtcctggt tttgatgaac aagctcaaag gaaactcaga acttggtatc aattgatgga
     3241 tctggctgtt tcagaacaaa aggctttctt atctagacac cagttaattg accgtacttt
     3301 gcaaaatggc atggcttcct ccaaaaactt gataacctct acagaatggc acgattacaa
     3361 cccgaatggc aatgtcctta tcgtcacacc gcaatacttg gagcgtcaaa acattcctgt
     3421 agatacaact attgagcaaa agatgaatca ccttgatgta ggggagtttg tcttattgct
     3481 gcctgaacac ctccgttcag aggaagaaca ttataaatct gtttttgaag acgacttaac
     3541 cagtcgcatg tctagtcaag atgaacgaca gcaaatgact gctacggtag gttatttaga
     3601 atcaggtcag gatcgttttg tgtataatac gacccctatt tcttaccagc agtttttgaa
     3661 agatccaatc atcattgtta taacacccca atcaactggt ccacagtcca ttttgttttg
     3721 gatagacgca gtacagaact acgttctctt taatcaattg tctgatgccc aggagcttat
     3781 ccagagacaa ggcattgaaa attgggtctc agaaatgcaa acaggttacc acaactacat
     3841 cacattattg gataatattc agagggaacg ttgggtaatg ctagcaggag ctgggcttgg
     3901 gattgcaact tcaatcttgt tgtttaacac tatgaatagg ctctactttg aagaatttag
     3961 acgtgccatt tttatcaaac gcattgcagg tctcaggttc ttagaaatcc atcgcactta
     4021 tctctttgct caactgggtg tgtttttact gggatttgtt gcgagtgtat ttcttcaggt
     4081 agagatagga gttgctttct tagtcttgtt actctttact ggtctatctc ttttacagtt
     4141 acatgtccaa atgcagaaag aaaacaagat gtccatgctt gttttgaagg gaggttaata
     4201 tgattgaact taaacaggtg agtaaatctt ttggagaacg agagttattt tcgaatcttt
     4261 caatgacatt tgaggctgga aaagtctatg ccttaattgg ttcaagtggt agcggaaaaa
     4321 caaccttgat gaacatgatt gggaaattag aaccttatga tgggacgatt ttttaccgag
     4381 gtaaagactt ggccaattat aaatcgagtg attttttccg tcacgaattg ggctacctct
     4441 tccagaactt tggcttaatt gaaaaccaaa gtattgaaga aaaccttaag ctaggtctca
     4501 ttggtcaaaa gttgagtcgg tcggaacagc ggttgaggca gaagcaggct ttagaacagg
     4561 tcggcctgac ttatcttgac ctagataagc gcatctttga gttatcgggc ggagaatcgc
     4621 aacgggttgc cttggcaaaa gttatcttaa agaatccacc ctttattctg gcagatgagc
     4681 caacagcttc aatagaccca gcaacttctc agttgattat ggagattctg ctatctcttc
     4741 gagatgataa taggctaatc attatcgcaa cacataatcc agcaatttgg gagatggctg
     4801 atgaagtgtt cacgatggat cgtctgaaat aaaaatcctt gtttttaatt gcacgatgag
     4861 ttactgaaat attatcataa atcaataatt ggagttaatt tagaattgta ctttattaat
     4921 attgaggtaa ctttttcttg ataaaggaag aaatagtgga gaggaagtta gaatgaaaaa
     4981 attcgacaat tatattgttg agaagccttg cgattctaat tcagataaac tgcaaaaaat
     5041 cttaataatt gaaaatttgg tagatgatat tttgcaattt cctctcagaa tcaataatag
     5101 tgtaggagag attttcctcc tacaaccgtt tcaaaagaaa actatcttta ttccatgtta
     5161 ttttgaggaa gatattgtga aagtcaaaga tgatgataaa gttgagtgga atttgttaga
     5221 atttcaaaaa tttagagcat ttttggctta gtaatctgtg ttgaaggctc aaaacctatg
     5281 gtaaaaaagt cgctttgaaa acgtattgcc tccaaagatt tagttaaata atgatttaac
     5341 acaaaaagaa attattgaag ttctggaaag atgttgtttc agtattgaga aaaggtggga
     5401 aaaacttgcg attttcacag agaaaggaag aaaaagtata gaaatatagt caattgaaac
     5461 aagaacagga caaaagaacc ttttgtgcca tatttttctc ctttcgcttt acaattggct
     5521 tgaacacctt tattgtatcg cgtttggagt ttttttggta taaccttcga cgcgcacccg
     5581 catagcgggt gtttttttgt ctcgcaccta acggagcgag acgaactaat agtcacttaa
     5641 tcaaaaaacg caccatatca aaaactaaaa agtttgatat catgcgtcat gtcttaaact
     5701 aattgactat actttctatt caaatgagct tttaaccaat tgattgagcc aatccactct
     5761 taaaaccaaa gagcaatttc tcgcttagct gactcttctg aatctgaacc atgtacaaca
     5821 ttttggataa cctcattttc tccagcagct tttgcaaaat cacctcgaat agtgcctggt
     5881 aaagcttctt ctggacgagt tgcacccatc atggtccgcc aagtttcgat tactttggga
     5941 ccagaaatga cacccacaag aactggacct gaagtcatga attcacgaat cggtgggtaa
     6001 aaactctgac caaccaagtc ctgatagtgc tggtcaatca actcttctga aacttgtgaa
     6061 cgaaactcca atttttcgat tgtaaatcca cgttgttcga tgcgctttaa cacttcaccc
     6121 actagccctc tttttacacc atctggtttg atgataaaga atgtttgttc catacccgtc
     6181 tcctttgtca gcttctttct tttattttac cacatttcgt ggaaaaatgg agaaagtttt
     6241 cagaatagag aatgagagaa cccgagggtt ctctcattct ctattattct actgtttctt
     6301 ccacagtttc aacggcagta tccacaacta cttctgttgt ttcttcattt ccttcttcct
     6361 ctactggagg attaaggtat tcttcttcgt tgacagcata tggttcaagg ttacggtaac
     6421 gggccatacc agtaccagct gggatgatct taccgatgat aacattttct ttaagtccaa
     6481 ggagatggtc tttcttacca cggatagctg cgtcagtaag gacacgagtt gtttcctgga
     6541 aggaagccgc tgacaagaaa ctgtttgttt caagtgaggc tttggtaatt cccataagga
     6601 ctgggcgacc tgtcgctgga actccacctg cgataaggac atctttgttg gcatctgtaa
     6661 agtcattgat atccatgagg gtacccatga gaagatctgt atcacctgga tccatgacac
     6721 ggactttacg gatcatttga cgaaccatta cctcgatgtg tttgtcaccg atttctaccc
     6781 cttggctacg gtaaactttt tgtacttcac cgagaaggta cgtttcaact gacaagacat
     6841 cacgaactgc aaggagacgt tttggttgga tagaaccttc tgtcagagca gcaccacgcg
     6901 ctacttggcc cccaacttcg acacgcatac gagctgtaaa tggaacgaca tattcacctt
     6961 cgccagtttc acccttaaca aagactttct tggtacgagt tgatgcatct tcttcgatag
     7021 cagtaacttc tcctttaacc tctgtgataa ccgcttcccc tttaggattg cgggcttcaa
     7081 agatttcttg gacacgagga agaccctgag tgatatcggt atttgaggca accccacctg
     7141 tgtggaaggt acgcattgta agctgtgtac caggttcccc gatagattgg gcagcaatgg
     7201 taccaactgc ttcaccaact tcaaccgcat caccagtcgc caagttgata ccgtaacagt
     7261 gacggcagac accgtgacga gtgttacatg taaatacaga acggatagtc acttcttcca
     7321 caccagcatt gacaatttca cgcgccttgt cttctgtaat caattcattt ggaccaataa
     7381 tcactgcacc agtttctgga tgtttaacag ttttcttagt gtaacgaccg ttgagacgct
     7441 cttcgagaga ctcgatcatc tcttttcctt ctgcgataga acggatcaag agaccacggt
     7501 cagttccaca gtcgtcctca cggatgataa cgtcttgggc aacgtcgacc aaacgacgag
     7561 tcaagtaacc tgagtcggct gtcttaaggg ccgtatcggt cataccttta cgagcaccgt
     7621 gagttgagaa gaacatttcc aataccgaca aaccttcgcg gaagtttgaa aggattggca
     7681 attccatgat acgtccattc ggagcagcca tcagaccacg cataccggca agctgtgaga
     7741 agtttgagat gttaccacgg gctccagagt ccatcatcat aacgattggg ttcttaggat
     7801 cttggttagc aatcaagcgt ttctcaagtt tttcacgggc agcacgccat tcagctgtaa
     7861 cagcattgta acgctcgtcg tctgtgatca taccacgacg gaattgtttg gtgatttgtt
     7921 cgacacgttt gtgtgattct tcaatgattt cagtcttgtc atcaacgact gggatatcgg
     7981 caatacccac tgtcaatcct gcaagagttg agtggtggta accgaggttc ttcatgcggt
     8041 caagtagggc agaagtttct gtcgtacgga aacgtttgaa gatttcagcg atgatatttc
     8101 caaggttttt cttcttgaat ggagggttga gctcaagatt gctgatagct tccttgatat
     8161 ctccaccaag tggcaagaag tatttagctg gaacaccttc tgtcaagttg gcattgtttg
     8221 gttcttgcaa gtatggtagc ccctctggca tgatatcgtt gaagagaatt ttaccaactg
     8281 ttgtaagcaa gaccttatgt ctttgctctt ctgtccaagg cttgttgagg ctgtctgttg
     8341 cgataccaac acgtgagtgg aggtgaacat aaccattgcg gtaagccata accgcttcgt
     8401 cacggtcttt gaagaccatt ccttcacctt cgcgaccagc ttcttccatg gtcaagtagt
     8461 agttacccaa aaccatgtcc tgagatggag taactaccgg tttcccatct ttcgggttca
     8521 agatgtgctc agcagctagc atgaggatac gagcttctgc ttgtgcttct tctgaaagtg
     8581 gtacgtggat ggccatttgg tccccgtcaa agtcggcatt gtaggcttca cagacaagtg
     8641 ggtgcaagcg aagagcctta ccatcaatta agactggctc gaaggcttgg atacccaaac
     8701 ggtgaagggt cggtgcgcgg ttcaaaagca ctgggtgttc tttaatcact tcttcaagga
     8761 tatcccagat acgctcatct ccgcgttcca ccaagcgttt agctgctttg acgttttgca
     8821 cgatatcacg ggcaacgatt tcacgcatga caaatggttt aaagagttca atcgccattt
     8881 cacgtggcac accacattgg tacatcttaa gagttggacc aacggcgata acggaacgtc
     8941 ctgagaagtc aacacgttta ccgagcaagt tttgacggaa gcgtccttgt ttacctttaa
     9001 gcatgtggct caatgatttc aatggacggc tacctggtcc tgtgattgga cgaccacgac
     9061 gaccattgtc aatcaaagcg tcaactgctt cttgaagcat acgtttctca ttttgaacga
     9121 tgatacctgg tgcatttaac tcaagcaaac gagccaaacg gttgttacgg ttgataacac
     9181 ggcggtaaag gtcattcaag tcagatgagg caaaacggcc accatccaac tgcaacattg
     9241 gacgaagatc tggtgggata accggaagga tgttaagaat catccattca ggtttgtttc
     9301 cagacttgta aaaggcatcc aaaacatcca aacgacggat ggctttgaca cgtttttgtc
     9361 cagtagctgt tttcaattct tctttgagtt cagcaatttc tttttcaaga tctacttgct
     9421 tcaaaaggtc ttggatggct tccgcaccca tcttggcaac aaatgaacca taaccatatt
     9481 cgcgcaagcg ctctcggtat tcgcgctctg tcatgataga cttgtgctca agtggtgtat
     9541 ccttaggatc aatcaccaca taagccgcaa agtagataac ttcctcgagg gcacgagggc
     9601 tcatatcaag ggtcaagccc atacggcttg gaatcccctt gaagtaccag atgtgagata
     9661 caggagcttt caattcgata tgtcccatac gctcacgacg aactttcgta cgcgttactt
     9721 caaccccaca gcggtcacaa acaattcctc tgtaacgaat gcgtttgtac ttaccacaag
     9781 cacattccca gtcttttgta ggaccaaaga tcacttcatc aaagagtcct tcacgttctg
     9841 gtttcaaggt acgataattg attgtttcag gttttttgac ttctccataa gaccatgaac
     9901 ggactttact tggagaagct agggtgattt gcatactttt aaaacgattt acatcaacca
     9961 ctatttcttc cctttctatt ctaagtgaac tgcttattcc tgttcagcag cttcttctgt
    10021 tgcttccgct tttgttgctt tctcagcttc ttcagcttca aaggctgctt tagcctcttg
    10081 ggctgctttt tcgcgggctt tttcaaggtc atctacgtgg atgacatctt cgtccattcc
    10141 ttcatccaag tcgcgaagtt ccacttcttg gtcatcttcg tctaggacac gcatgtcaag
    10201 accaagagat tgcaattctt tgacaagaac tcggaaggat tctggaacac ctggttttgg
    10261 aattggtttg ccttttgtaa tagcttcata ggctttcaaa cgtccgttga tatcgtcaga
    10321 cttgtaagtc aagatttctt gaaggacatt tgacgcaccg taggcttcaa gagcccaaac
    10381 ctccatctca ccgaaacgtt gtccaccaaa ctgagcttta cctccgagtg gttgttgggt
    10441 aacagttgag taaggtccga ctgaacgcgc atgcaattta tcgtcaacca tgtggtggag
    10501 tttgatcatg tacatgactc caacagaaac acggttatca aatggttcac ctgtacgtcc
    10561 atcgtaaagg attgtcttgg catcgctatc catacctgct tctttaacag ttgaccaaag
    10621 atcttcagaa cttgctccat caaagactgg tgtcgcaatg tgaataccaa gagtacgagc
    10681 tgccatacca aggtgaagct ccataacctg accgatattc atacgtgatg gcaccccaag
    10741 tgggttcaac atgatgtcga ctggagttcc gtctggaagg taaggcatgt cttctacagg
    10801 aacgatacga gagacaaccc ctttgtttcc gtgacgtccg gccattttat ctccgacctt
    10861 aatcttacgt ttttgagcga tgtaaacacg aaccaacatg ttaacacctg attgcaactc
    10921 atctccattt acacgtgtaa agatcttaac atcacgaacg acaccatcgg caccgtgtgg
    10981 tacacgaaga gaagtatcac gcacttcacg agacttgtct ccaaagatag cgtgcaagag
    11041 acgttcttca gctgaaagat ctttctcacc cttaggtgtt actttaccta caagaatatc
    11101 accttcttta acctcagcac caatacggat aatccccatt tcgtcaaggt ctttgagggc
    11161 atcttcacca acgtttggaa tttcgcgagt gatttcttca ggcccaagct ttgtatcgcg
    11221 cgtttctgat tcgtattctt caaggtgaac agatgtgtag acatcgtcct tcaccaagcg
    11281 ttcgctcatg ataacggcat cctcgaagtt gtagccttcc caagtcatgt aggcaacgat
    11341 tgggttttgt ccaagcgcca tttctccatt ttccatagaa ggtccgtcag cgatgaaatc
    11401 gcctttttca acgacatcac caacttttac aagagtgcgt tggttgtaag cagtacctga
    11461 gtttgaacga cggaattttt ggatgtggta aacatccaat gaaccatctt cacgacgaac
    11521 ttctaccttg tcagcatctg cgtaagtaac tttaccatca tactgagcaa tcacagccgc
    11581 accagaatcg tgggctgctt ggtattccat accagtacca acgtaaggtg cctgaggatt
    11641 aatcaatggc acagcctgac gttgcatatt ggctcccatg agggcacggt tggagtcatc
    11701 gttttccaag aaaggaatac atgctgtcgc aacggcaact acctgttttg gtgaaacgtc
    11761 catgtagtca acaatattag ctggatactc ttggttgacc ccttggtgac gtcccatgac
    11821 aatcttctca gcaaaggttc catcttcttt cagacgagag ttagcctgag ctacagtata
    11881 ttcatcttct tcatcagctg tcaaccaaac aatttcgttc gtgacaacac ctgtttcacg
    11941 gtcaacctta cggtatggtg tttgaacaaa accatatttg ttcaagtgtc cgtaagatga
    12001 caagttattg atcaaaccga tgttaggtcc ttcaggtgtc tcgattggac acatacgacc
    12061 atagtgagtg tagtgcacgt cacgtacttc atatccggca cggtcacgag tcaaaccacc
    12121 aggtcctaag gctgacaaac ggcgtttgtg agacaactca gaaagcgggt tgtgttggtc
    12181 catgaactgt gacaactgtg atgaaccaaa gaattcttta actgcagctg ttacaggacg
    12241 gatattgata atttgttgtg gtgtcaagac ttcattgtcc tgaacagaca tacgttcacg
    12301 gacattacgt tccatacgag aaagtcccaa acgtacttgg ttggcaagca attcaccaac
    12361 cgcacggata cgacgatttc caaggtggtc gatatcatct acacggccaa gtccttcagc
    12421 caagttgagg aagtagctca tctcagcaag gatatctgca ggagtcaccg tacgaacctt
    12481 gtcatctggg ttagcattac caatgatcgt tacgacgcga tctggatcag ttggagcaac
    12541 aaccttgaat ttttgaagaa caacaggctc agtcacaacg gctgcatcgt ttgggatgta
    12601 gacaatcttg ttcaagtcgc catccaaatg gctttcaatg ctttcaatca cgctacgagt
    12661 cataatcgta ccagcttcta ccaagatttc tccagtttca gggtctacca atggctctgc
    12721 aatggtttgg ttgagcaaac gtgttttaac attgagtttt ttattgattt tgtaacgacc
    12781 aactgctgcc aagtcatagc gacgtgggtc aaagaagcga gccacaagca agctacgtga
    12841 gctttcagct gtcttaggct cacctggacg aaggcgttcg taaatttctt tcaaggcttc
    12901 gtctgtacga gagtccattg gattcttgtg gatatctttt tcaacagtgt tgcgaaccaa
    12961 ttcgctgtca ccaaagatat caaagatttc atcatcacct gagaaaccaa gagcacgaac
    13021 caaggttgta aatggaatct tacgagtacg gtcgatacga gtgtaggtga tatcttttga
    13081 gtcgctttca agttccaacc aagctccacg gttagggata acagttgaac catagcccac
    13141 cttaccattt ttgtctactt tgtcgttaaa gtaaacacct ggtgagcgga ccaactgaga
    13201 aacgataata cgttcaccac cattgatgat gaaagtaccc atttctgtca tgattgggaa
    13261 atcaccaaag aaaacttctt gggtcttgat ttcgcctgtt tctttattga tcaagcggaa
    13321 ggttacaaaa attggtgctg agtagctagc atcgtggata cgagcttctt ctagcgtgta
    13381 ttttggttcc ttgatttcat atccaacaaa ttccaactcc attgtgtctg tgaagtttga
    13441 aattggcaat acatcttcaa acacttcctt aagaccgtgg tctaggaaag ctttgaatga
    13501 gtcagtttga atttcaatca aatttggtaa gtcaagaact tctttgattc ttgaaaaact
    13561 acgacgggta cgatgtttcc cgtattgaac gtcatgtcct gccaagatga ttctcctttg
    13621 taaataagtt ccaagccttg tcaatcaggc ttttctaatc gccatatggt tgtaaacccc
    13681 ttatcaccgt gtcctcttga cgaattttca gaatctttaa gcctctgtta caaatgctca
    13741 aaatcttgaa aaaaagcaca aaaagagcag ctaaatctga ctttttcaga agatttaact
    13801 gctgtgagcc ttgtctggac aatatttcag acaaaaccta cgacaaatga ttacccatat
    13861 tataccctat ttagctagat ttttcaaggg gattcagtag gtttttggta atttttttcc
    13921 catagaaaac ttggcatcac attcgaatca cgctatggta caaaaaactg aaaaaactat
    13981 tgactgaaaa tcattttcaa ggtataataa taaacgttaa ggcggtatag ccaagtggta
    14041 aggcacggct ctgcaaaagc ttgatcgtcg gttcaaatcc gtctaccgcc ttctataact
    14101 tgatttatca ggtttcaaat gaacagaaag cccaatttga agggcttttt ttattttccc
    14161 tcgaataaat acgtataact ttaaaaactt ttggagcgag tttgtggcag agttctttcc
    14221 atggcataat tcccttttga aatcaattga aaaaagcttc gtcacgcctg gtgacaaaag
    14281 cctctagttt attctgtttc ttcttctatt tcgacttctg tgatttggac ttttaccttg
    14341 gtaacacgtc cattcttcac tttatcattg gttaagataa actgtttgtt ttggctgacc
    14401 aattcatagc tgagtttctc agtcgttgga atggttccca ctcctgtcaa ataataacca
    14461 gcgatagtat caacgtcatc actttccagt tcaacattaa agtaattatt gaaatcattg
    14521 agggtcatag ttccttgaac aatgtaggta tcctcgccaa tctgatgaac atctattgct
    14581 gctttgtccg tttcatcatc aatttcacca acaatttcct ctaagaggtc ttccaaagtc
    14641 accaaaccag ccataccacc gtattcatcc agcaagattg ccatttgtct ttgggtattt
    14701 cgcagttctt ttagcaagtc atccacaaaa atagtttcag gtacaaaaag tggatcttgt
    14761 aaaattctct tccaaacaat attgtcaaaa ccgtccacaa agcctgcctt aaggagactc
    14821 ttggtgtgaa tgattccaat tacattgtcc ttatccccat cataaaccgg gatacgagaa
    14881 taattttgtt ttaaaatact ttggataatg gcttgactat catcctgaat atccaccata
    14941 aaggcatccg ttcgaggaac cataacctct cgtgccatca gttcatcgag cgaaaagaca
    15001 ccttgtaaca tctcaatctc atcagcatcc aatgtttctt cactatttgt cagcatatag
    15061 gcaatttcat cacgggtcat cttttcatcc gcatcatcga atgacatagg agtcaaatgg
    15121 ctcaagaaat tggtcgaagc agctaaaagc caaacaaaag gactgactag ttttccgatc
    15181 ccaatgataa ccggcgctgt acgaattgcc aaggcatcct ttagattaag agcgattctc
    15241 ttaggatata attccccaaa aacgatggaa atataggtca aaaatgccaa ggatagaaaa
    15301 gttgccacgg cttgtgctgt ttcgccattc ccaagccaag aggcaatcac acgtcctaga
    15361 gtatcagtta aactcgcccc tgataagatt gtaatcaggg tgattcctac ctgaatggtt
    15421 gataaaaagt ggttaggatt ttctagtacc ttcagcaggc ggatgtagcg tctgtctcct
    15481 tcttccgcct tttgttcaac tcgggcacga ttaagagaaa ccatggccat ttctgtggct
    15541 gagaaaaagg catttaatac cgtcaagata aacaataaaa caaattgtag caacaaattc
    15601 tgactacttg ggtcttccat agttcttctc ctaaaatagt atcttatcct ttattatatc
    15661 acaaaattta atccaataca tattattttt ttcttagcgt aacttccatc tctctcccaa
    15721 aactggaagt tagtctaaga cggatttcca ctgatttcac gcgtttttct catactaaaa
    15781 gctaggttgt gggaaactgg aagttagttt tagaagttac tattttttct tagtacctgt
    15841 tctagccctg cttaaactag taaaaagaac tcctacatcc ataagtgaca taggagttta
    15901 ggcttatctt ttactgagtt acagttactt ctccagttcg gtaatccagt tgaagtccct
    15961 tttgaacatt gggaactaga acattgaatt ccaattctcc atccgaagac ttggcttcaa
    16021 tctgtgaagc tgagggaact aaatcctcgt ttgaagcgta gtaaagggtt acacggtaac
    16081 ggacacgctt gttttggtcc aaggctttac gcaccttgct ttcatagtag ttttgaccag
    16141 tcgaatactc ggcttgtgcc tgatttgccc aggctgtctg aacagcaatg tttttaggat
    16201 tgcttgttga ggcatcaaaa ccatccaaac caccgattaa ggcatagcct aacaaatgac
    16261 ctctatcgac tgcatgggta taagagccct ttagattctt gacctgatgc caacctggag
    16321 gagtccaaga agttgaacca ttcccagttt ctttacgatt cttgtactga cgagtggcct
    16381 tagacaagag ggcattagct acggttggaa cagtttcctt acccactgtc tttgttttat
    16441 tgtcagcgta gggcttactt gaaaccttgg catctagatt tgttttatta ccattgacga
    16501 taaaagcacc tgagccattc cactccagac tcccctttat ttgactcttg actgcgtctg
    16561 ttaagacact ctctgccaat gcttgactag gagcttcagg cgcttgtttt ttctgactaa
    16621 gcttggtttt aggactatta gctgtcgact gcatctgctt gatataatag ctccctgtag
    16681 acaaaagcaa taacactagc agtccgatta gtgtctgtct tgtttttttg ttcatatttc
    16741 tccttatctt tagaaaaagg ctggttctcc agcctaattt cctgtaaatt tatgaatcaa
    16801 ttcctgccat tttgctggag acaggatagc ccatgaatct tgacccttgc ccaagattcc
    16861 ataacctacc attaaaccga ttcctagagc cagagtacct aaaatcagta ctatgatgac
    16921 taaaagtaaa cgcttgacta cgtagcttga tttcttattc atcttcatca cttgcctcaa
    16981 tccgctgaat cttagcacct agctgcgcca acttctcatg gaaaccgtag taacctctat
    17041 ccaagtgaac caatttaccg accacagttt ctccctgtgc taccaaacct gtcaaaatca
    17101 aagctgcact ggcacgaagg tcagttgaaa gaacttctgc tccctgcaaa ggctgtccac
    17161 caacaatacg agctgtatca cggataatct cagaatgcaa gcccatgcgg cgcatctctt
    17221 ctaggtgttg gaaacgattt tcaaaaactg tctccaccat ggttgattcg ccttttgcaa
    17281 ctgtcatcaa ggctgtaaat tgagcctgca tatctgttgg aaatcctggg tggggcaagg
    17341 ttttcacatg aacagctttt agattttcca gttgagaacg aacacgaatt ccttcgtctt
    17401 cttcaattac ttcaacaccc atttcaagta acttggcaat caagggacgg ttgtgctccc
    17461 agacagcgtc tcgaatcaag acatcaccac cagtcatggc agcagctacc ataaaggttc
    17521 ctgcttcgat acggtcctgg actacattgt gagtcgtacc atgaagtttc tcaacaccag
    17581 taatggttat agtctctgta ccagcacctt tgaccttggc tcccatttca ttaaggagaa
    17641 tggctaagtc aacaatctca ggctcacgcg cagcattctc aatcactgtc accccatcag
    17701 ccagagtcgc tgccatcatc aagttctgcg ttgcaccaac acttggaaag tccatataga
    17761 tatgagcacc atgcaagcgt tctgcctttg cttcgatgta accagctgtc tgactaatct
    17821 taacccccat agcttccaga cctttcaaat gaagatcaat aggacggcta ccaatcgtac
    17881 aaccacctgg catggatacc ttggcatgac ccacacgggc aaggattggc cctaatacaa
    17941 cgatggaggc gcgcatctta ctgacatact tgtaaggggc ttcctcagtg atgtcgccag
    18001 tagcatccac cttgacaaga tgagcttcct catcaaagtc aaccttggca ttcaaaccac
    18061 caactacctg attcataata aagacatccg acaaaatcgg aacattctgc aagacggtct
    18121 ttccttcact tgctagaata gtcgctgcca acaagggtaa gactgcattt tttgctccct
    18181 cgatcgtcac gcttcctacc agacgattat cgccaccttg aaccacaatt ttatccatac
    18241 tcgtttcctt tactcttgat tttataataa tcctctaagc gcttcttgcc acaaatgata
    18301 caggctaata aagaaagaac tcaggatgta gcctatgaca atgctaaaaa aggctactaa
    18361 tacacgaact tttcttgtat tctcagctgt cacttttaaa accttttccc atctaacaag
    18421 atcctttaaa aggtaaaaac tcacataaat aaagagcata tgactactta gagtgaataa
    18481 taattgaacc atttgactat tataccatct tctctgtttg tgcgctagtt tggaaacttc
    18541 actcaaaaat tagggattgt ttttcaagta atcaatcgca tcttgtaggg ttttaacggg
    18601 cacgattttc atatccgtct tgattgtttt agccgcttct agggctgttt ggtagttgtt
    18661 tttcgcgtcc ggatgcgcct tttgttcttc ttcgctaaca gggttatcag gagcaaagaa
    18721 aatagcagca cctgccctag ccgaagctac aaccttctta tcaatacctc caatgtctcc
    18781 cacattacca tcgcggtcaa tggtacctgt accggcaaca atacgaccat tacgaagatc
    18841 tgggtgagct atttgagtat agatagctag actaaacatg agaccagcac ttgggccgcc
    18901 aataccagct gttgaaaagc taattgggac attgctgatt acctctgtac gatcaatcaa
    18961 gccgattcca atcccatttt ttccattttc caaggtgata atcttccctt ctgcagactt
    19021 ggtttgccca tcctcttcat aggtgacctt gacggaatcc cctaattttt gagaactgac
    19081 gtaatcaatc aagtctttgg aactatcaaa ggtctgatca ttgactgctg tgactgtatc
    19141 agagatattg agaatccctt taaaggttga attatccgtc acattcaaaa cataaactcc
    19201 aaagtacttg agttcgatat ccttaccagc tgtttttagt ccttgatact tggccatatt
    19261 ttgcgatgtt tgcatgtaga attgattgat tcgcataaat tcaacatcgg aagaaccacc
    19321 tgtagtctcc tgagcactac gaatatctgt aaaaggtgtc aaccaagcat aaatcatatg
    19381 agctaaagta gcatgttgaa caccaaccgt aacgaattga taggcaccag cttccttatc
    19441 ttctgtgtca tttactttaa ggacttggcg aatatcttcc gaaccacctg gaacctctat
    19501 ataataaggc aagggcacta caaatgccaa gaaagtcaca atcaaggccg caatgacgta
    19561 taagggccat ctaatctttt ttttcatttc ttatttcctc caaaatactc tcgggaacat
    19621 agcaggcaat atcctgacca aacttcaaaa gctctctaac gcctgatgaa ctgatataga
    19681 gatgttcagg tcgactatgt aaataaatag tctctatatc agaagacagc tgatgattgt
    19741 agtaatcaaa actggcttca tattgcaaat ccgacgcatt cctcaaacca cgcactagac
    19801 aagtagcacc caatcttttt gcaacatcga ccaccaattc atcatgagaa gccacgactt
    19861 caacattttc caaatgtccc aaagcctttt ctagcccccg tttacgattt tcaagaggga
    19921 gaaatccttg tttgtgggga ttaaaaaaaa tacccacaca aagcttatca aaaagtctgc
    19981 tcgcccgttc aatgatatcc agatgcccat ttgtcatcgg atcaaatgag cctgtgaata
    20041 agccaatctt atctgacata gactgtcacc ttactaattc cataaatctt ttccttccag
    20101 atacccagac aggcaatttc ttctggaagt tcaacggtct tatccgtctc gcacacaacc
    20161 ataacatctt cagaaaaaag ctctctctca gccatttttt caatatctgc tacgatttgt
    20221 tccttggcat agggagggtc taagaaaacg aggtcaaatt ccccagatac ctgttccaat
    20281 gccctttctg catccatttt gaggagttga aattttccaa cttccttggt catctggata
    20341 ttttcagcca cgatggtctg agccttacgg tctcgctcca ccaaaacagc actggacatg
    20401 ccacgcgata ctgcttcgat agataaacca ccactacctg cataaaggtc caagactcgt
    20461 cccacttcaa agtagggacc aatcatgtta aaaatggctc ccctaacctt atccgaagta
    20521 ggtcttgttg tcttgccttc tagtgtcttg aggggacgtc ccccatagat tcctgatacg
    20581 attttcatac tgtttattat accaaattat agacaaaaag agaaagaaaa ccgaaccttg
    20641 cggttcgatt ctctacaaaa tattttcgta agtatcgcgg acttcttgag gccaaacact
    20701 tgtttgcact tctccgatgt gtctcttgcg aagtaggaac atggccatac gagattgtcc
    20761 aattcctcca ccgattgtca atgggaatag gccattcaac aaagacttgt gccattccaa
    20821 ttctaagcgg tcttcatcac ctgtaatttc cacctgacgt ctaagagttt cttcatctac
    20881 acgaattccc atagaagaca actcaaaggc tccacctaaa gactcattcc agacaagaat
    20941 atcaccattt agacccttgt agccattctc agactcgctt gtccagtcat catagtctgg
    21001 tgcacgtcca tcgtgcggtt taccatctgg caactcgcca ccgataccaa tcaaaaagac
    21061 ggctccaaat tctttacaaa tcgcattttc acgttcttta ggtgtcaagt ctgggtagcg
    21121 ttctaccaat tcttctgtat ggataaaggt gatttgtttt ggcaagatag actcgatgtc
    21181 atagcgggct tcaacagcta gctcagtcag gcgaatagcc ttataaatct tctcaactgt
    21241 ttcttttaga taaacgatgt tacgcttacc atttgggata accttctccc agtcccactg
    21301 gtcaacataa acagagtggg ttgcatccaa ggaatcctca tctggacgaa gggctttcat
    21361 gtggacaaag agaccctctc cttcaccaaa gccaaaacga gccaaggtgt ggcgtttcca
    21421 tttagcaagt gagtgcacca cttcataagt agcatcaggg atttggagaa ccttgaccga
    21481 tactggattt tccacaccag acaggttgtc ctgcattccg tcaccgacct tactcaagat
    21541 aggaccttga acttcgacaa cttctagctt atctttcaaa tactgggtaa aagtgttttt
    21601 gacaaaggaa atttcttctt gttgatggat aaaacttttc ttcatagaca actcctcaaa
    21661 aatttaataa acgtattata cacctatttc caagaaaaga aaagagaaaa ttttaaaaaa
    21721 atcagtgtca ctcaaaacac tgatttcata gaccttttag tcattgcgac caaagatacg
    21781 tagaatactt aggaaaagat tgataaaatc aagatagatg ctgagagcca ttgacaccac
    21841 ccagcctgtc gctacacgac cttgtgattg ttcataggcg aggcgaatct tttggttatc
    21901 ccaagcaatc aaccctgaaa aaaccaaaac catggctacg ctaatcatat aatcaaagaa
    21961 gccactagcc aagaaaatat tgactaccat agcaatcagt aaaccaatga gagctgccat
    22021 cattgctcga ccaatcccac tcaagtcttt cttggtaaaa ataccaactg ccgccatgac
    22081 aaagaagaga agggcgctcg atacaaaggc agataaaact gtacccggag tatagaaagc
    22141 caccacaaaa ctaagggtaa agccgtttaa aacggagtaa agtaaaaata ctggaagagc
    22201 agctggacta tttcttgaag ccatgctact ggcaacgaaa accaaagcca actccgcaaa
    22261 agtagcaatg gtcaaccaga gacgcccctg catcaacaag taaaccaact gagactgaaa
    22321 gaccgtcaac ataaggcctg ataccaaagc agatagtccg attcccagac caacaaaggc
    22381 ataaacctta gcgtaaaatt gattgagacc tgcacggtca tgtataatag tgtgattcat
    22441 ccttttctcc ttttctgtga acatctttcc ttgattataa caaaaaaagt taaaattagc
    22501 ttaaatggaa gattaaaata ccggctgcaa ctgcaacatt caaactctcg gcctgcccct
    22561 tcatgctaat atggaccaac tggtctgcac tttcagccat aaggggacta attccttgac
    22621 cctcatttcc cataactagt acaaaatttt ctatagaagg cagttctctg taatcaacag
    22681 aatctttaga tagggttgtt gctagcactg ggatagctac ctttttagtt tcgtcaagaa
    22741 gcgcttgact agtcatccgg taaatgggca gatggaaatg actaccttgc atggaacgta
    22801 gggttttgag actgtagatg tctgccgact tatctgaaac aatcactcct gtaaaacctg
    22861 ctgcatccgc agttcgaatg atagttccta cattaccagg atcttgcaca tcttccaaaa
    22921 acaagaactt gccctgactc aaatcagctt gtcctacttc ttctttttga accacggcaa
    22981 caattccctg tggagtctga gaatccgcca aatctagcaa aatatcctct gaaatccaga
    23041 cagtttgcgg aaaagcagct aactgatttc gataattttc tagggcaaag attttctcaa
    23101 tcgtcactcc agcttggaca gcttcttcaa acaagtgcca gccttcaatc aaataggcag
    23161 acttgcggta ttttttttgg tgtaatttct tggcattttt taccacagaa ttggcttttg
    23221 aggttataat agtcatagaa atattataac acaatcaaag gggtttggta tgcaaaaggt
    23281 tagaatgatt gcccaaggta gggtgcaggg agtcggcttt cgttggggtg tttatagcct
    23341 ggcccttgaa atcggtggca tcacaggtcg ggtatggaat aacgacgatg gcacagtgga
    23401 aatcttagcc caagcagact catctgctat catggcaaaa tttatccaag aaatccgaaa
    23461 aggaccgaca cctttttcaa aagtaagcta cttagatgtc aaactaagca actttcctcc
    23521 ctactctgac tttaaaatcg caaattaggt ctccagaact attgtatatt ttgtaaaaaa
    23581 acagtagaat agaaaggtat aatttttaaa gaaggaataa aaacagtgaa atctattaaa
    23641 cgttttgcat tctcggctat gggagtggct atgttgcttg tcttgactgg ctgtgtcaat
    23701 gtcgataaaa ccacaggtca gccaacagga tttatttgga atacgatcgg agcgcctatg
    23761 gctgaagcca tcaagtactt cgctactgat aaaggtctag gctttggtgt cgctatcatt
    23821 atcgtaacca ttatcgtacg cttgattatc ttaccacttg gtatctacca atcatggaag
    23881 gcaacgcttc actctgaaaa gatgaacgct ctcaagcatg tccttgagcc acaccaaaca
    23941 cgtctcaaag aagcaactac tcaagaagaa aaactcgaag cccaacaagc cctctttgcc
    24001 gctcaaaaag agcacggcat cagcatgttt ggtggtgtag gatgtttccc tatcctcctt
    24061 caaatgcctt tcttctctgc tatctacttt gctgcccaac atactgaagg ggttgctcaa
    24121 gcaagctacc taggcattcc tctaggttct ccaagtatga ttttggttgc ctgtgctggt
    24181 gtcctttact atcttcaatc gctcctttca cttcacggag tagaagatga aatgcaaaga
    24241 gaacaaatca agaaaatgat ttacatgagc ccactcatga tcgtcgtctt ctccctcttc
    24301 tcaccagcta gtgtcacact ttactgggtt gtcggtggtt tcatgatgat tctccaacag
    24361 tttatcgtca actatatcgt tcgtccaaaa cttcgcaaaa aagtccgtga agaactagcc
    24421 aagaacccac caaaagcaag tgctttctct aaaccaagtg gacgaaaaga cgttacccct
    24481 gaacaaccaa ctgctatcac aagcaagaaa aaacacaaaa atcgcaacgc tggaaaacaa
    24541 cgttcgagat aagaagaaaa aggcttagct gatttgctaa gtctttttgc aaatcaaaag
    24601 cccgatgttt ccatcaggct tcttttctat ccatgtacac gtttcatata atcttgataa
    24661 ctttcggtat ccatgagttt tttagcgttc ttgacgcgat ctgctgttgg tggtttgact
    24721 ccttcgaggg aatatggaat tccaagttca cgccacttga actcacccat ggtgtgataa
    24781 ggtagaattt caaacttatc aacatttttg agggtcttga cgaacttacc aagttcaatc
    24841 aagtcatcat ctctgtctgt caatcctgga actagcacgt ggcgaatcca gacaggtttt
    24901 ccaatatctg atagatactg ggcacaagcc aagatatttt tattagtttg gctagtgaca
    24961 atcttgtgct gttcttcgtt gatttccttg atatccaaaa gaaccaagtc agtgacagcc
    25021 atgagtttgt caaacttctc aaggtaacgt ggtttattac ggaaaggaag agcacaggta
    25081 tccaaggtac agtggattcc ttgttcctta gccttggtga agagagcaat caggaaatca
    25141 atctgcaaga gagcttctcc tccactgact gtaatcccac ccttatttcc ccagaaacca
    25201 cggtagcgca aggcctctgt caagacatca tctaccgtcc gttcacgtga cttattggac
    25261 tccatagccc aagtgtctgg gttgtggcaa tactggcaac gcatgtgaca gccctgcaaa
    25321 aagacaataa agcgaatacc aggcccatct actgacccaa agctttctgt cgaatgcacc
    25381 attcctgtca cttgtccata atcaattgtt tcttcagaca tatcgttacc ttccttgaaa
    25441 acgttttata gttttattat atcacgactt gaaggaaaaa caagattttt gcctattttt
    25501 tagaaagcaa tctgtttttc aatttcattt tcaatctatt atcagattat tcttcaaaat
    25561 cgaagccata taaggttgca aaatagtcct cagggcgctc tgcacggcgg atttgacggg
    25621 ctttaccttc ttcggtatag aggatttccg cagaacgtaa tttggcatta tactggtagc
    25681 ccattgaaaa tccgtgggca cctgtatcat gaattaccag caaatcaccg atttctgtat
    25741 gaggcagttc gcgattgact gcaaatttat cattgttttc acagagtgaa ccaaccacat
    25801 ctaccgcttc agctggtcca tctggatggg tcacgttgct aatatgatgg taagctccgt
    25861 acatagctgg acgcatgagg ttgactgctg aggcatccac acctagatag gtacggtagg
    25921 tttccttctt atgagtcact cttgtgacta gagcaccgtg aggtgccagc ataaaacgac
    25981 ccaattcggt gaaaatcttg acctgaccaa gacctgctga cgtaagaact tcttcataca
    26041 ccttacgaac tccctcacca atcaaggcga tatcgttcgg ctcctggtct ggacgataat
    26101 taacaccaat accgccagaa agattgataa agtctagcga aatgcccaac ttttccttga
    26161 tttcaacagc cagttcaaag agctgacgag ccaactctgg ataatagaga tgggtcacgg
    26221 tattggacgc taggaaggag tgaatcccaa aagtcttggc tcctttttcc ttcaagatag
    26281 caaaggcttc aaagagctgg tccttggtca tgccaaactt agcctcccca ggattgtcca
    26341 taatgtctgt ccccagttca aaaacgcctc caggattata acgacaagag atgatttctg
    26401 gaatgcctgc tactctctcc agatgttcaa tatcttcaaa ggcatccaag ttaatggtcg
    26461 cacccaattc acgtgcatag gcgtattcct tgtctggcgt gttgttggaa gagaacataa
    26521 tctcagaacc cagaaagtcc agtttatggc tcatcaaaag ctctacataa ctagagcagt
    26581 ccacaccaca accttcttct tggagaattt tcaaaatagc tggagttgga gtagccttaa
    26641 ctgcaaaata ttccttaaag cccttgttcc acgaaaaagc ttggttgacg gctcttgcct
    26701 tctcacgaat ccccttctca tcatacaagt gaaagggagt cgggaactcg gcaacaatcg
    26761 cttctaactc ttctctattg ataaatggtg ttttcataag cttccttcta ttctgtttgc
    26821 aaagaaaggc tgggacaatc gttccaacct ttagttctat ctcttattta tcttcgtcaa
    26881 agatattgcc aacctcaaca tcgatggctt ggttcttgac atcaatattg gttaccttca
    26941 agagtgactt gtagtcaaac tgcttttcac gttcttcttg ggcattgtcc gcaaagaccg
    27001 ctacacctgc cagttctgag tcgaactcgt gcaagagact aatcatacca ttgaccgttc
    27061 cgccaccttt caagaagtca tccacaatca agacacggct gcctgcctta agactacgtt
    27121 ttgaaaggaa cattttctcg atacggtcac cacttgaacc tgaaacatag ttgacgctaa
    27181 cagttgaacc ttcggtaatt ttcaggtcac ggcgcacaat gacaaaagag acattgagga
    27241 cattggcaac tgcatttgca agtggcacac ccttagttgc tacggtcata accgcgtcaa
    27301 ttttttggtc cataaagctt ttggcaataa tacgaccaat atttttcaag atggctggtg
    27361 tgctaagcaa atcagacaga tagatataac cacctggcaa gatacggtca ctttctgaca
    27421 acttggtacg caagtcttca accatttcct tagcatcctg actcgaaata gacggtgtga
    27481 agatgacacc tccgccagcc ccagtcactg tctggatatg accgatttca atttcctcaa
    27541 aggcgcgttt gataatgacg atatcttctg agatggatga tttagcagac tcatactttt
    27601 cagcaaaagt attgagacta gttagtttat aaggattatt aatcaaatag ttggaaatga
    27661 caaccatccg atcacttctt cttaatttca tttctatttc ctattccatt tttattcaaa
    27721 aaatcaaaaa gcaaactaga aagctggtcg ctggtggttc aaaacactgt tttgagattg
    27781 tcaatagaac tgacaaaccc tgtaatatac ctgcatatat acatacgaca aggcgatact
    27841 accctagttt gaagagattt tcgaagagta ttcatttttg tcttttactt attatctttt
    27901 acttattata ccatattcac ataaaaaaac gaacattctt atcctaaaaa atgctcattt
    27961 ttcttaaatt atcaatctaa atctggttta tagaaggaac gattatccat agcgaagatt
    28021 ttattggtca tctctccttt atccaccaaa gccagagctg ttgacatcat catcatgctt
    28081 gcatccagat tgtcaatcat atggataatc tctgcctcca taatacgtgg acggactgga
    28141 cttccatatt caagcaagcc gtggtgactg aggatgacat gacgaagcaa aacgacttct
    28201 tccttggtat catcgatgcc gagttccata actgtcttgg taatttcgct atcaatgaga
    28261 gcgatatgtc caagaagatt acctcgcact gtgtactctg tctggtctgg ccccgtcaac
    28321 tcgataacct tagctaagtc atgcagcata atccccgcat agagcaggct cttattgagc
    28381 tgaggataaa cttcgctaat agcgtctgcc aaacgtacca tggtcgccgt atgataagcc
    28441 aaccccgttt caaaggcatg gtggttggtc ttggcagctg gataggagta gaattcctta
    28501 tcatacttgg tgtagagatt tcggacaatc cgttgccaga caggattttc aattttgaaa
    28561 atcatttgcg acatgtagtc acgaatttcc ttgacatcaa ctggtgactt gaccttgaaa
    28621 tcggctgggt cattgggttc accagcttga ggcaggcgga gagtaatttg attgacttga
    28681 ggggtattgt tataaacttc tcggcgtcct ttcatgtgga caaccttacc tgcggtaaag
    28741 gcctcaatgt tatgaggttg ggcatcccag agcttcccat caatctcgcc actatcatct
    28801 tggaaggtaa aggctaggta gtttttccca gctcgagttt gcctcaggtc agctgatttg
    28861 attaggtaaa agccttcaaa tagctcatct tttttcatgt gactaatctt catattcttc
    28921 ctcattttct tgaaaatgga gtagatcaag cgcaggctca ccttctgaca actcaatgtg
    28981 acggagcgtc cgctcgatag ctatggtacg acggtttaat aattcatcaa tattgccaga
    29041 ggcatgttgg agatgttttt gtgccttgac cagaatacca ccaaacttgc caaactcggt
    29101 cttgacactg gcaagagtct tgctgatatg gtcggcactc ttttggatat taagggtctt
    29161 gaaaccaact gatagagagt taagaagggc tgatagggta cttggtcctg caacaataat
    29221 ctgttcttcc cgtctcaaat catcaaagaa gaccggattg cgcacaatct ctgaataaag
    29281 accttctgtc ggaacaaaca agattccaaa gttggttgtc cgaggtggtg ctatgtactt
    29341 gttcctaata tccctagcaa agcgcttgac gcttgctagg agtgacttac gacagcgttc
    29401 aatctcatcc ttgtcacctg tctcataggc ttcttccaag cggtaataat ctgccagtgg
    29461 aaacttagag tcaattggca gatagacgta ttcttggtcg ccttgtccgg gtaacttgat
    29521 ggcatactcc actcgttcac tagagttttc aaccgttgcg tattctcgtt cgtactgggc
    29581 aggtgtcatg atgtcttcaa taatttgccc cagttgcaat tctcccagaa tccctcgcgt
    29641 cttggttcca gagagaacct tgttaagagc tccgacatca cgggcaactg tctgcatttc
    29701 tccaaggcca cgattgacag actccagttg tttagaaact gtctcaaagg aagcctgtaa
    29761 gcgtgtctgc aaggtctttt ctagtttttc ctcgaccgtc tggcgcattt gttccaaacg
    29821 ttgctcattt gattcctgca aggcttggag acgttggtct gtcttgtctc tagtttggag
    29881 gagattatct gtcatttctt gacggacttg ggtcagacct tggtgcaatt caatccgcac
    29941 ttcttgcaaa cggtcgctga caaccacttc caaatctttt tggtctaact ggctggcttg
    30001 tctggcttgg tcaaagcggt aatccaactg gtctgacaaa tgatctgcct gatcctccaa
    30061 gctcttactt aagtgtttct cctgcctatc ctgcctttgc caaatcagaa agagaccagc
    30121 tagattggca attaataata gaataagtaa actttccatc ctacctcctg tccttgctat
    30181 gcagtacgac cacataacca tctgggcaag ttaccgacac ttccctatct atatattcgt
    30241 tagaagcgta cactttttta aagaaaaaat tttcctctgt caactcatac ttggctccaa
    30301 gaatagtcag ctggctatcc cgaactggca taaaggctag atagtcgtag tctgaacgag
    30361 gttctagctg actgattcct tctggacaat aagtaatcaa gttttgccca tcctcaattt
    30421 ctatttgatg catataggat gccaacttag gattgctagg cagaaagaca ttggccaaca
    30481 tatggtcaat acggccaccc aaggcaccga aaatagtaac ctgagcctga ggattttgct
    30541 taaagatggt caagagagcc aattccaaat cagtatcatc tttttctggc cgggcttgga
    30601 caaaatactg ggcacgtttt tgaatcacct gtcgctcttc ttccgtcaca gaatcaaaat
    30661 ctccgactgc tagagcaaga ggcaaatctt cttccaagat ccagagcgag cctcgatcca
    30721 caccaacaaa accatcaaag tccgtccgat aatgaccgcg gtctccgcct gcaaaaacgg
    30781 caaccctagt ccagttgttt tctgagagtt tgtactcgct cattgacatc tcccttaaag
    30841 acataggaac ctgctacaaa aacggtcgca ccagcttctt tggcttgagc aatagtttgg
    30901 tcatcaatcc caccatccac ttcaatctca aagttcaaac ctttttcatc acgaagggca
    30961 accagctcac gaaccttatc catggtttct ggcagaaagg cttgaccacc aaatccaggg
    31021 ttaactgtca tgactaggac ttggtcaact agatgaagca catgcttaat cgcttcaact
    31081 ggtgtaccag gattgataac aactgatggc ttgacaccga gtgaacgaat cttttggagg
    31141 gcaccatgaa tatgaggcgt tgcttccaca tggatactaa taatatccgc acctgcacgc
    31201 acaaagtcct ctaaatgatg ctcagggttt gacaccatca agtggcagtc gaaaaccatc
    31261 ttactatgag gacgaagagc ttcgaccaca cccgcaccaa aactgatttg cggcacaaaa
    31321 tgaccatcca taatatcgat atgggcatac tctgccccag ttgcttctag gcgtttaatt
    31381 tcacgttcaa agttggcata atctgctgcc agaattgacg gagcaatctt gtattgagac
    31441 ataggtttct ccttattttg gaattttttt gctgactttt ttataggttt ctctgcgatt
    31501 ttcaatttcg ctgaggaatt gcaggtagtt gtcaaaacgg aagggcgcaa tggtaccttc
    31561 ttcaactgct ggcttgacag cacaggacgg ctcatgggta tgggtacaag tacggaattt
    31621 gcagtctcga ctgacagtgg caatctctgg aaaggcctga ttgaggtctt cagccgttga
    31681 tacttcataa tccagtgatg aaaagcctgg tgtgtctgcg attttacccc cattgaggtt
    31741 gtaaaaacta acagctcgag tggtatgacg cccgcgaccc agactgtctg aaatttctcc
    31801 cgtttcaaga ttaagatctg gtgcgatttt attgagaaga gttgatttcc caacacccgt
    31861 ctgccccata aagaccgtaa ccttgcctgt taacaaaggc aggagttcct ctttactggt
    31921 cacaaagtca tatccgatgt caccataggt ctgctggtaa aaatccagtt ctcccccgtc
    31981 ttccaacaaa tccattttag aaatatagac gatgggatga atacccttat gctccaaaag
    32041 aaccaagaaa cgatccagca aattgctgtt aaaatcaggt tccttgacag acatgattac
    32101 tacagcttgg tcgatattga caataggcgg acggaccaga ctgtttttcc gttcgtgaat
    32161 cttgagaata tagccttctg aattttcctc ggcagagaaa tctacccagt ccccaacgta
    32221 aggggtatgc ccttttttac ggaaattccc acgcgcgcgt gtttgataga cttggccatc
    32281 actctctaca tagtagaacc cagccaaggc tttaatgatt tgtccctgca aaataactcc
    32341 tttaaaattg ttagttccat tataacaaaa aaaagctatt ttagcttagc ttctctttac
    32401 aatttcgata agtctattct aaaagaaaaa gatacactca cgctttagat accaagtttt
    32461 atacaaaaat aacgaatcta cctccttata taaaaacagg cgaacagaga gcctaaaact
    32521 ccctgttcgc tttacttttt ctgtgtgtaa tagaaaaaac ctaataaagc aagacaaaaa
    32581 atatgaatat aaactccata catcatgtgg ggaaagacag actctataaa atagcctggt
    32641 agatagatga ttggaaattg atagaaatca taggtcgtta gagatagtaa acaaagaaca
    32701 tacataaata ctgctacaat tagaacccat ccttgctttc gccatagaaa aatcatcaat
    32761 aagaacagac aaagtagata gattgaccaa tttcgcaaca taaattgaaa accataaatc
    32821 atactttcag tcgtttctcc atcaatcaca tcaactactt tatagaaagg taaacaatag
    32881 ctcactaaaa aaagaagggg actaattatt tttttcatat tctctaccat ggattatttt
    32941 gcaagtaagc tgaccatcta tctccatatc gaatcaaatt aacagtataa ttagttacta
    33001 cccgattttc acctggtcca accttagaaa aacctgtgct atactcccaa ctacttcttg
    33061 cacctggatg aaaccatttt tgctgggatg aataccaacc accttgtcta acttccacac
    33121 ttgaagattc tatttcgata tccctttcat ggaagtcaac tcctccactt attttagtaa
    33181 tatttttttg tacacctcca tcataactca cccagtgaac aacagtatac aattccacac
    33241 tataagtact atcaaactga ctttgatgag tagtaatgtt ttgagtataa gcgtgcactt
    33301 tctgtatacc aaaaatatgg tctaatatat tcgttggttt agcaagctta ttgtaaccct
    33361 cttcaacaaa agtagccttc tctaaatcag cggtataaac agtttttcct tgtactactt
    33421 gattgtttat agaagtaata cggctatcca caacaatggg ggccaccata tctccatcgc
    33481 tagtttctat tttagctgcc tctttcacct catatacaac tcgattttct tcagactgaa
    33541 tcacttctac cccatttttt tcaggtatag ataatctagt ttgcccatta gaaaccgtta
    33601 tatttggacc aatattcttc tcatttgcat aagtcgcaat aggaaatcct gatagaacca
    33661 ctgcacacat aaggctattg actaattttt ttctcatgaa tatatccttc ccattttcaa
    33721 gaggagtaaa aatagctaat ataagatgaa cgtttcctta tatttcagaa taggatatct
    33781 atctgaaagc aattcacact cctatagata actgttccaa atcttggcta catatatttc
    33841 aatagtttgc tacaaaaaac tcctttctaa gtctattgaa catgccctta ttcacctact
    33901 aatatatcac aaaaataaat aaatacaaga ctgttttttc tactagtttt atgatagaat
    33961 agtattgtta gggtaaattt catatataaa tgtcgagtga aaaatcgatg gagcagaaat
    34021 cggtagacaa agactcgatt tcgttggtcc agccccacaa ggctaatcag gtatttcacg
    34081 agtttaaaaa tctaatgaag tgcccaatca actactgatt cattgattta atacgaacta
    34141 tagtggattg aacctagaaa tagtacacta aggattctaa aacgtttcta gaaattaatt
    34201 tgactttcct aatctctttg ttcatatctt atttcaatct actatatata ccaaaatgct
    34261 aggacaaaag tctttaaaat cagagaaacg catattatca ggtgttggaa aaccttgata
    34321 atatgcgttt tatcatagaa agatctactt cattttttac taaaattaag tttttgtccc
    34381 tgcatcttag agtccttgcc ctttaagtgc atctgctagg ctggcaaact cttccaagct
    34441 gagagcttcc ccacgcacac ttggtgacaa gcctgcttgg tccaaagcct tggtcagctt
    34501 gtccttgacc tcttcagtct tgccaaagta acctgtcaag ttattccaca aggtcttgcg
    34561 gcgatgggta aaactagcct tggaaacctt aaagaaaaag ttctcgtctt ccactgctac
    34621 agctggcact ggacggcgca ccattttcaa gatggctgag tctacatttg gtgctggcac
    34681 aaagactgta cgcggcacga taaaggcaac cttggctgtc atgtaatact gcacagcgat
    34741 agacaagcta ccgtaagcct tggtatttgg ttgagccgag atacggtccg ccacttcttt
    34801 ttgcatcatg accacaaact cactaaaagg aatgccactc tcgattaagt gcatgagaat
    34861 aggcgttgtg atgtagtaag gcaaattagc cactaccttg attggcaggt caggattttt
    34921 gaaattctgg atatgttgcg ccaaatcgac cttgagaatg tcctcgttga ctacagtcac
    34981 attgtcaaaa tcacgcaagg tatctgccaa aatcggtacc aaacgatggt cgatctcaaa
    35041 ggccataact tctgctgcac gctcagccaa aaactcggtc aaggcaccaa tacctggccc
    35101 gatttcaatg acattgacct ggtcatcaat ctcagccgta tccacgattt tttgtaggat
    35161 attggtatcc gtcaagaaat tttgcccaaa ggacttttta aaggtaaaac cgtgacgctc
    35221 cagcactgcc ttggtcacgc tataatctgc aattctcatc tattctcttt tctattatct
    35281 gttactacta ttgtaacaca ttccccttgt attttggggg tgaaaatcat accaaaaaac
    35341 tatctttcct catgaagaaa gatagtcttg gaaatcttta aaagtggcct tagcgcttct
    35401 ctcgaaacat acgttcatac tcttcgaaaa tctcttcaaa cc
//
LOCUS       DAVBCG010000007        34373 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBCG010000007 DAVBCG010000000
VERSION     DAVBCG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34373
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.007"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            25..264
                     /locus_tag="VQ495_000288"
     CDS             25..264
                     /locus_tag="VQ495_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2530302.1"
                     /translation="MPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHEDSQFK
                     GDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            608..1942
                     /gene="trmFO"
                     /locus_tag="VQ495_000289"
     CDS             608..1942
                     /gene="trmFO"
                     /locus_tag="VQ495_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2530303.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2026..2763
                     /locus_tag="VQ495_000290"
     CDS             2026..2763
                     /locus_tag="VQ495_000290"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2530304.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2772..3329
                     /gene="frr"
                     /locus_tag="VQ495_000291"
     CDS             2772..3329
                     /gene="frr"
                     /locus_tag="VQ495_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2530305.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3389..4243
                     /gene="cvfB"
                     /locus_tag="VQ495_000292"
     CDS             3389..4243
                     /gene="cvfB"
                     /locus_tag="VQ495_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2530306.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4252..4467
                     /locus_tag="VQ495_000293"
     CDS             4252..4467
                     /locus_tag="VQ495_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2530307.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4553..5521
                     /locus_tag="VQ495_000294"
     CDS             4553..5521
                     /locus_tag="VQ495_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2530308.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5701..6201
                     /locus_tag="VQ495_000295"
     CDS             5701..6201
                     /locus_tag="VQ495_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530309.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6204..6464
                     /locus_tag="VQ495_000296"
                     /pseudo
     CDS             6204..6464
                     /locus_tag="VQ495_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6831..7942)
                     /gene="ald"
                     /locus_tag="VQ495_000297"
                     /pseudo
     CDS             complement(6831..7942)
                     /gene="ald"
                     /locus_tag="VQ495_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8119..8688
                     /locus_tag="VQ495_000298"
     CDS             8119..8688
                     /locus_tag="VQ495_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530310.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8756..9406
                     /locus_tag="VQ495_000299"
     CDS             8756..9406
                     /locus_tag="VQ495_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2530311.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9390..11630
                     /locus_tag="VQ495_000300"
     CDS             9390..11630
                     /locus_tag="VQ495_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2530312.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11810..11998
                     /locus_tag="VQ495_000301"
     CDS             11810..11998
                     /locus_tag="VQ495_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530313.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12030..12617
                     /locus_tag="VQ495_000302"
     CDS             12030..12617
                     /locus_tag="VQ495_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530314.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12621..13796
                     /locus_tag="VQ495_000303"
     CDS             12621..13796
                     /locus_tag="VQ495_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530315.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    13970..14101
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14117..14647
                     /gene="infC"
                     /locus_tag="VQ495_000304"
     CDS             14117..14647
                     /gene="infC"
                     /locus_tag="VQ495_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2530316.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14680..14880
                     /gene="rpmI"
                     /locus_tag="VQ495_000305"
     CDS             14680..14880
                     /gene="rpmI"
                     /locus_tag="VQ495_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2530317.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14932..15291
                     /gene="rplT"
                     /locus_tag="VQ495_000306"
     CDS             14932..15291
                     /gene="rplT"
                     /locus_tag="VQ495_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2530318.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15349..15729
                     /locus_tag="VQ495_000307"
     CDS             15349..15729
                     /locus_tag="VQ495_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2530319.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            15979..16779
                     /locus_tag="VQ495_000308"
     CDS             15979..16779
                     /locus_tag="VQ495_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2530320.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            16742..17728
                     /locus_tag="VQ495_000309"
     CDS             16742..17728
                     /locus_tag="VQ495_000309"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2530321.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17770..19242
                     /locus_tag="VQ495_000310"
     CDS             17770..19242
                     /locus_tag="VQ495_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET2530322.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAANEWIWDKESWFYLKFDGKIAEKEWVYDSHSQAWYYFKSGGYMTANEWIWDKESWF
                     YLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENA
                     AYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDL
                     QALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKL
                     ENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAH
                     SALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRG
                     RTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(19303..20958)
                     /gene="pavA"
                     /locus_tag="VQ495_000311"
     CDS             complement(19303..20958)
                     /gene="pavA"
                     /locus_tag="VQ495_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2530323.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21063..21560
                     /gene="ybeY"
                     /locus_tag="VQ495_000312"
     CDS             21063..21560
                     /gene="ybeY"
                     /locus_tag="VQ495_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2530324.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            21541..21936
                     /locus_tag="VQ495_000313"
     CDS             21541..21936
                     /locus_tag="VQ495_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2530325.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            21953..22852
                     /gene="era"
                     /locus_tag="VQ495_000314"
     CDS             21953..22852
                     /gene="era"
                     /locus_tag="VQ495_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2530326.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            22901..23725
                     /gene="mutM"
                     /locus_tag="VQ495_000315"
     CDS             22901..23725
                     /gene="mutM"
                     /locus_tag="VQ495_000315"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2530327.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            23725..24330
                     /locus_tag="VQ495_000316"
     CDS             23725..24330
                     /locus_tag="VQ495_000316"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2530328.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24317..25516
                     /locus_tag="VQ495_000317"
     CDS             24317..25516
                     /locus_tag="VQ495_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2530329.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            25516..25665
                     /gene="rpmG"
                     /locus_tag="VQ495_000318"
     CDS             25516..25665
                     /gene="rpmG"
                     /locus_tag="VQ495_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2530330.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            25705..25938
                     /gene="secG"
                     /locus_tag="VQ495_000319"
     CDS             25705..25938
                     /gene="secG"
                     /locus_tag="VQ495_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2530331.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26040..28394
                     /gene="rnr"
                     /locus_tag="VQ495_000320"
     CDS             26040..28394
                     /gene="rnr"
                     /locus_tag="VQ495_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2530332.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28357..28824
                     /gene="smpB"
                     /locus_tag="VQ495_000321"
     CDS             28357..28824
                     /gene="smpB"
                     /locus_tag="VQ495_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2530333.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NIKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            28840..29700
                     /gene="tehB"
                     /locus_tag="VQ495_000322"
     CDS             28840..29700
                     /gene="tehB"
                     /locus_tag="VQ495_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2530334.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            29773..30726
                     /gene="coiA"
                     /locus_tag="VQ495_000323"
     CDS             29773..30726
                     /gene="coiA"
                     /locus_tag="VQ495_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2530335.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            30745..32547
                     /gene="pepF"
                     /locus_tag="VQ495_000324"
     CDS             30745..32547
                     /gene="pepF"
                     /locus_tag="VQ495_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2530336.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            32549..33262
                     /locus_tag="VQ495_000325"
     CDS             32549..33262
                     /locus_tag="VQ495_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2530337.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33329..34270
                     /gene="prsA"
                     /locus_tag="VQ495_000326"
     CDS             33329..34270
                     /gene="prsA"
                     /locus_tag="VQ495_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2530338.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT        10336 a         6404 c         7774 g         9859 t
ORIGIN      
        1 tgcttcataa ttcatagaac tactatacca tattttgttt cgcaggaagt ctattggaaa
       61 gtaagaaata ttggagctga ggcgattaga agaaattgtg agcgtggtgc tattttttca
      121 ggtaaaataa aacatcacga agattctcag tttaaaggag accactatgt tgaatgttat
      181 gctgttttag ataatacggt tatagcaaga gatagaataa cagtccctat cgatccgtta
      241 tgtggaaaag attttataga gtagcatata attgattctt aactggaata ctctctatct
      301 ctttacatca agaaaatgac taaacaggga agtttgcctt cttccctttt tttgttatac
      361 tagtagaaga aaaaaataga aagatttgtg ggagtgaaaa agtccggggg actttttcag
      421 cttgagccaa gaaattcgag agcgaggaaa gtccggggga ctttttcagc ctgagccaag
      481 aaattcgaga gcgaagtaag tccaatggac tttttcagcc tgagccaaga aactcgaaag
      541 ctcgtaagtc tgattggctt tttcaatgtg aatcttaact tcacactccc aaagaggtat
      601 tagtgtcgtg tctcaatctt atatcaatgt tatcggtgct ggtttggcag gttctgaagc
      661 agcttaccaa atcgcagagc gtggtattcc agttaaacta tatgaaatgc gtggtgtcaa
      721 gtcgactccc cagcataaaa cagacaattt tgctgagttg gtttgttcca attctttgcg
      781 tggggatgct ttgacaaatg cagttggtct tctcaaggaa gaaatgcgtc gcttgggttc
      841 tgttatcttg gaatctgctg aggctacacg tgttcctgca ggtggtgccc ttgcagtgga
      901 ccgtgatggt ttctctcaaa tggtgaccga aaaagttgcc aaccacccct tgattgaagt
      961 ggttcgtgat gaaattacag aattgccgac agatgttatt acggttatcg ctactggtcc
     1021 tttgacaagt gatgccttgg ctgaaaagat tcatgccctt aatgacggtg atggttttta
     1081 tttctacgat gcggcagcgc ctattatcga tgtcaacact atcgatatga gcaaggtcta
     1141 cctcaaatca cgttatgata agggagaagc ggcctacctc aatgccccta tgaccaagca
     1201 agaatttatg gatttccatg aagctttggt caatgcagaa gaagcaccgc ttaattcttt
     1261 tgaaaaagaa aagtactttg aaggctgtat gcctatcgaa gtcatggcca aacgtggcat
     1321 taaaactatg ctttatggcc ctatgaagcc agtcggtctt gagtacccag acgactatac
     1381 aggacctcgt gatggagaat ttaaaacacc ttatgcggtt gtgcaacttc gtcaggacaa
     1441 tgcagctggt agcctctaca atattgttgg tttccagacc cacctcaaat ggggagaaca
     1501 aaagcgtatc ttccaaatga ttccgggtct tgaaaatgca gagtttgtcc gttatggtgt
     1561 catgcatcgc aattcttaca tggattcacc aaatcttctt gagcagactt accgttctaa
     1621 gaaacaacca aatctcttct ttgctggtca gatgacgggg gtggaaggct atgttgagtc
     1681 ggcggcttca ggcttagttg cgggaattaa cgcagctcgt ctcttcaagg aagaaagcga
     1741 ggctattttc cccgagacga cagcgattgg aagcttagct cattacatta cccatgccga
     1801 cagcaaacat ttccaaccaa tgaatgtcaa ttttgggatc atcaaggagt tggaaggcga
     1861 gcgtatccgt gataagaagg ctcgttatga aaaaattgca gagcgtgccc ttgccgactt
     1921 agaggaattt ttgactgtct aatttttttg aaagaattgc tcatgatact ataaaaatct
     1981 tagaaattgt gataaaatag gtaggatgaa agaaggagag tgaaaatggc gaatcccaag
     2041 tataaacgta ttttaatcaa gttatcaggt gaagcccttg ccggtgaacg tggcgtaggg
     2101 attgatatcc aaacagttca aacaatcgca aaagagattc aagaagttca tagcttaggt
     2161 atcgaaattg cccttgttat tggtggagga aatctctggc gtggagaacc tgcagcagaa
     2221 gcaggtatgg accgtgttca ggcagattac actggaatgc ttgggactgt tatgaatgct
     2281 cttgtgatgg cagattcatt gcaacaagtt ggggttgata cgcgtgtaca aacagctatt
     2341 gctatgcaac aagtggcaga gccttatgtc cgtggacgtg cccttcgtca ccttgaaaaa
     2401 ggccgtatcg ttatctttgg tgctggaatt ggttcaccat acttctcgac agatacaaca
     2461 gcggcccttc gtgcagctga aatcgaagcg gatgccatcc tcatggctaa aaatggtgtc
     2521 gatggtgttt acaatgccga tcctaagaaa gataagacag ctgttaagtt tgaagaattg
     2581 acccaccgtg acgttatcaa taaaggtctt cgtatcatgg actcaacagc ttcaaccctc
     2641 tcaatggaca acgacattga cttggttgtc ttcaacatga accaatcagg caacatcaaa
     2701 cgtgtcgtat ttggtgaaaa tatcggaaca acagtttcaa ataatatcga agaaaaggaa
     2761 taagaaagaa tatggctaac gcaattattg aaaaagctaa agagagaatg acccagtctc
     2821 accaatcact tgctcgtgaa tttggtggta tccgtgctgg tcgtgccaat gcaagcttgc
     2881 ttgaccgtat acatgtagaa tactatggag ttgaaactcc tcttaaccaa atcgcttcaa
     2941 ttacgattcc agaagcgcgt gttttgttgg taacaccatt tgacaagtct tcattgaaag
     3001 acatcgaacg tgccttgaac gcttctgatc ttggtatcac accagctaat gacggttctg
     3061 tgattcgctt ggttgtccca gctcttacag aagagactcg tcgtgacctt gctaaagaag
     3121 tgaagaaggt cggcgaaaat gctaaagtgg ctgtccgcaa tatccgtcgc gatgctatgg
     3181 acgaagctaa gaaacaagaa aaagcaaaag aaatcactga agacgaattg aagactcttg
     3241 aaaaagacat tcaaaaagta acagacgatg ctgttaaaca catcgacgat atgactgcca
     3301 acaaagagaa agaacttttg gaagtctaaa aataaacaga aaaactcagt tggcattgct
     3361 ggctgagttt tattcgaaag aaggaaatat gaatacaaat cttgcaagtt ttatcgttgg
     3421 actgatcatc gatgaaaacg accgttttta ctttgtgcaa aaggatggtc aaacctatgc
     3481 tcttgctaag gaagaaggcc aacatacagt aggggatacg gtcaaaggtt ttgcatacac
     3541 ggatatgaag caaaaactcc gcctgacaag cttagaagtg actgccactc aggaccaatt
     3601 tggttgggga cgtgtcacag aggttcgtaa ggacttgggt gtctttgtgg atacaggcct
     3661 tcctgacaag gaaatcgttg tgtcactcga tattctccct gagctcaagg aactctggcc
     3721 taagaagggc gaccaactct acatccgtct tgaagtggat aagaaagacc gtatctgggg
     3781 tctcttggct tatcaagaag acttccaacg tcttgctcgt cctgcctaca acaacatgca
     3841 gaaccaaaac tggccagcca ttgtttaccg tctcaagcta tcaggaactt ttgtttacct
     3901 accagaaaat aatatgcttg gttttattca tcctagcgag cgttacgcag agccacgttt
     3961 ggggcaagta ttagatgcgc gcgttattgg tttccgtgaa gtggaccgca ctttgaacct
     4021 ctccctcaaa ccacgctcct ttgaaatgtt ggaaaacgat gctcagatga ttttgactta
     4081 tttggaaagc aatggcggtt tcatgacctt aaatgacaag tcatctccag acgacatcaa
     4141 ggcaaccttt ggcatttcta aaggtcagtt caagaaagct ttaggtggtc ttatgaaggc
     4201 tggtaaaatc aagcaggacc agtttggtac agagttgatt tagggaggct tatgagaaaa
     4261 tcattttaca cttggctcat gaccgagcgc aatcctaaaa gtaacaatcc caaagcaatt
     4321 ttggcagacc tcgcttttga agaggcagct tttccaaaac atacagatga ttttgatgag
     4381 gtcagtcgct ttttggagga gcatgccagt ttctctttta acctaggaga ttttgacagc
     4441 atttggcagg aatatctaga acactagcat ttattcattg ggtttgggct agtaatttct
     4501 ccatccctct gctataataa aaagaaataa aaggattaga gaggttcttt atttgaagga
     4561 acattcaata gacattcaac tgagtcatcc agatgacctg tttcatcttt ttggttccaa
     4621 tgaacgccat cttcgtttga tggaagaaga gcttgatgtt gtgattcatg ctcgtacgga
     4681 gattgtccag gttttgggag aagagtctgc ttgcgaggaa gcccgtcagg ttattcaagc
     4741 tctcatggcc ttggtaaatc gtgggatgac cgttggtacg ccagatgtag tgacggctat
     4801 tagtatggtc aaaaatgatg aaattgacaa gtttgtcgcc ctttacgaag aagaaatcat
     4861 caaggataat actgggaagc ctatccgcgt caaaacctta ggtcaaaaac tttatgtgga
     4921 cagtgtcaaa cagcatgatg tgacctttgg aattgggcca gcaggtacag ggaagacctt
     4981 ccttgcagtg accttggcag tgactgccct taaacgtggg caagtcaagc gaattatcct
     5041 aactcgtcca gcggtggaag cgggagagag tcttggattt cttccgggtg atcttaagga
     5101 gaaggtggat ccttaccttc gtcctgttta cgatgccttg tatcaaattc ttgggaaaga
     5161 ccaaacgact cgtctcatgg agcgtgaaat tatcgaaatt gcgccccttg cctatatgcg
     5221 tggccggacc ttggatgatg cctttgtcat tctcgatgag gcgcaaaaca cgaccatcat
     5281 gcagatgaag atgttcttga cgcgtttagg ctttcattct aagatgattg tcaatggaga
     5341 tatcagtcag attgacctgc cacgtaatgt caagtccggt ttgattgatg ctcaagagaa
     5401 actcaagaac atccatcaga ttgactttgt tcatttttca gccaaggatg tggttcgcca
     5461 tccagttgtc gctcagatta tccgagccta tgaatattct actgaagttg cacacgactg
     5521 attttgagga agttcgcctg caaaagaata tacttttgag agaggaaaat ccagttgtat
     5581 aggctaaagg tttgagacaa cgattaggca cgatggaaag aacttttatg tggctgatga
     5641 cgatcagtgc atcttcctgt gtcataatca cagggcacaa gaaagtagga atttgaaaag
     5701 atgattgacc aactatctaa gtattacagt tgtaggatac taactgaaaa ggatattcca
     5761 agtattttat ctttatatga aagtaatcct ctgtattttc agcattgtcc accagagcca
     5821 aattttgcaa ctgtaaaaga ggacatgctt tgtctacctg aaggtaaagc taaggctgat
     5881 aagttttttg ttggattttg gaatggctct gaccttgtgg ctgttatgga ttttgtctat
     5941 gcatatcctg atgaggagac tgtttttatt ggtttgttta tggttgatca agcctatcag
     6001 aggaaaggga ttggtagtca tattgtgaca gaagcactag cttattttgc taagaacttt
     6061 cgaaaggcac gtttggctta tgttaaggga aatccgcaat ctcagcattt ttgggaaaag
     6121 cagggcttta aatcaattgg atgcgaggtt aagcaagaac tctatacggt tgttatcgct
     6181 gaacagagcc tagaagatta gaaatggcat caagtaagaa ctatttggaa tttgttttgg
     6241 aacaattatc aggattagat gatgtgactt accgttccat gattggggag tatattcttt
     6301 acttccgcgg caagattatt ggcggcattt atgacgatcg ctttttagtt aaacccgtgc
     6361 aagcagtctt agataagatt gaccaatctt cttttgagtt tccatacaaa ggtgccaaag
     6421 aaatgatttg agtggaagaa cttgataata agatgtttct ataagacctg attttagcta
     6481 tgtataacca actgccaacg cccaaaccta aaaagaaaaa gcaagggtga acgaagtaaa
     6541 aaagaagtct gctaaggccc tgtctttgca cgggtaaaat tttatatata aaaagaagct
     6601 gggactaaag agctcagctt cctttggttt atataattgt cattacaaga cgaagtggtt
     6661 gggcgaaact ctgttgactt tattcaattt agagtttctt atgcacaatt gagtctggaa
     6721 cgaaagtctc cagttgcaaa gtatacagta caataaacca acgatgtaat agctgatgac
     6781 acaaagcaca gtgggtagga cttgcgaagt cacccttttc ttttcaaaat ttatactaaa
     6841 tcattgatat cagtgtagtc acgattaagt ccttgagcaa ctggtaggct agtcaagtaa
     6901 ccttgataag tggtcacacc ttgacgcaag ccttcatctt cagagattgc ttgtgcgaat
     6961 cctttgccag ccaaagcttc gatataagga agagtgacat tggttagggc gatggttgaa
     7021 gtgcgggcaa ccgcaccagg gatattggca acggcatagt ggagaacacc gtgtttttca
     7081 tagacgggtt catcgtgcgt tgtcacacgg tcagctgttt cgataacgcc accttggtca
     7141 acagcaacgt caacgataca gagcctggac gcatttgttt gaccatctca tctgtcacca
     7201 attccggtgc ttttgcacca gggatgagaa tggctccaat caccacatca gcatctctca
     7261 cacttgcttc aatgttgaat gaattagata taatagtttg aatttgactt ccaaagactt
     7321 cttctagaac tgagagacgc ttggcactaa tatctaaaat agtcacttga gcaccaagac
     7381 caagggcgat gcgggcagca tgtgtaccga cgacaccatc accgatgata gttacttttc
     7441 cttttggaac acctggtaca ccaccaagta gaacaccaga gccaccagct tgcttagtaa
     7501 ggaagtgagc tccgatttga acagccatac gacctgcaac ctcactcata ggaacgagga
     7561 gcggtagttg tccttgattg tcacgaacag tttcataggc aattcctgtt gtttttgctg
     7621 ctaacatagc atctgctaat tctggagcag cggccatgtg caagtaggtg aagagaagaa
     7681 gatcgtcgcg caagtaaccg tattcagaac ttaaaggttc ttttactttc acaaccaact
     7741 ctgctgccca agcttcacca gcagtagcga caatctcagc tccttgcttt tgatagtcag
     7801 catcagtaaa gccagaaccg agaccagcat ttgtttcgat aaggacacga tgaccacgac
     7861 taactaagct atgaacacct gcaggtgtga gggcgacacg gttttcgtta tttttaattt
     7921 cttttgggat tccgattaac attgagataa cctacctttc aattgacggt cttgttttgg
     7981 ttgtcacatt ccagttcata aatcaaaaat gtgacggttt cattgtatat gaaaccgctt
     8041 caaaaatcaa gaaaaacttg tcatccaaat ttttttatgc tagactagtg aaaatcaagc
     8101 tctaatggag ggaaaagtat ggaatcaata tttgtgaaat ttgcccagta tccgtctata
     8161 gaaacggagc gtttattgct tagacctgta actttggatg atgcggaagc aatgtttgac
     8221 tatgcctcgg acaagggtaa tacacgttac acttttccaa ccaatcaaag cttggaagaa
     8281 accaagaata acattgctca gttctacttg gctaatccct tgggacgttg ggggattgaa
     8341 ttaaaagata acggcaagtt tatcggaact attgacttac acaagattga tcctgttctt
     8401 aagaaggcag ctattggcta cattatcaat aaaaagtatt ggaatcaagg attaacgaca
     8461 gaagccaatc gtgctgtgat tgagctagct tttgagaaga tagggatgaa taagttgact
     8521 gcccttcacg ataaggctaa tcccgcgtca ggaaaggtca tggagaaatc aggcatgcgt
     8581 ttttcccatg cagaaccata tgcttgtatg gaccagcatg aaaaaggccg aatcgtgaca
     8641 agagttcatt atgtcttgac caaggaagac tattttgcaa ataaataagc agttgaaaag
     8701 aaatttttcg actgtttttt cttcctctta cgaataatct aagagaggag aaaatatgga
     8761 agcaattatc gagaaaatca aagagtataa aatcatcgtc atctgtactg gtctgggctt
     8821 gcttgtagga ggatttttcc tgctaaagcc agctccacaa acatctgtca aagagacgaa
     8881 tttgcaggct gaagttgcag ctgtttccaa ggactcatcg accggaaagg aagtgaacaa
     8941 ggaagagaag gaagaaccag ttgaacaaga tctaatcaca gtagatgtca aaggtgctgt
     9001 caaatcgcca gggatttatg acttgcctgt aggtagtcga gtcaatgatg ctgttcagaa
     9061 ggctggtggc ttgacagagc aagcagacag caagtcgctc aatctagctc agaaagttag
     9121 tgatgaggct ctggtttacg ttcctactaa gggagaagaa gcagttagtc aacagactgg
     9181 ttcggggaca gcttcttcaa caagcaagga aaagaaggtc aatctcaaca aggccagtct
     9241 ggaagaactc aagcaggtca agggactggg aggaaaacga gctcaggaca ttattgacca
     9301 tcgtgaggca aatggcaagt tcaagtcagt agacgagctc aagaaggtct ctggcattgg
     9361 tgccaagacc atagaaaaac ttaaagacta tgttacagtg gattaagaat ttctctattc
     9421 ccctaattta cctgagtttt ctattactat ggctttacta cgccattttc tcagtatcct
     9481 attttgcttt gttgggtttt gtttttctgc tagtctgcct ctttatccaa tttccttgga
     9541 aatcagcagg taaagttcta gtgatttgtg gagtctttgg cttctggttt ctgtttcaaa
     9601 cttggcaaca gagtcaagtg agtcaaaatt tggtggattc tgttgaaagg gtacggattt
     9661 taccagacac tattaaggtt aacggtgaca gtctgtcctt tcgtggtaag tctgatggtc
     9721 gtgctttcca agtctattat aaactccagt ccgaggagga gaaagaagcc tttcaagctt
     9781 taactgacct tcatgagata ggactagaag ggaagctttc ggagccagaa gggcagagaa
     9841 attttggtgg ctttgactac caaggctatc tgaagactca gggaatttac cagacactca
     9901 atattaaaag aatccagtca ctccaaaagg ttggcagttg ggatataggt gaaaacctgt
     9961 ccagtttacg tcgaaaggct gtggtttgga ttaagatgca cttcccagac cctatgcgca
    10021 attacatgac aggactcttg ctaggacatc tggacaccga ttttgaggag atgaatgagc
    10081 tttattccag tttaggaatt attcaccttt ttgccttgtc aggtatgcag gtagggtttt
    10141 tcatggacgg atttaagaaa ctacttttac gattgggctt gacacaagaa aagttgaagt
    10201 ggctgactta tcccttttcc cttatctatg cgggactaac tggattttca gcatcggtta
    10261 ttcgcagtct cttgcaaaag ctactggctc aacatggggt taagggcttg gataattttg
    10321 ccttgacggt gcttgtcctc tttattgtca tgccaaactt tttcttgaca gcaggaggag
    10381 tcttgtcctg tgcttatgct tttatcctga ccatgaccag caaagaaggg gaggggctca
    10441 aggctgttac tagtgaaagt ctagtcatct ccttgggcat attgcccatt ctatccttct
    10501 attttgcgga atttcaacct tggtctatcc ttttgacctt tgtcttttcc tttctttttg
    10561 acttggtctt cttaccgctc ttgtctatct tatttgtcct ttcctttctc tatccagtca
    10621 ttcagctgaa ctttatcttt gaatggttag agggcattat tcgcttggtc tcgcaggtgg
    10681 caagtagacc gcttgtcttt ggacaaccca atgaatggct tttaatccta ttgttaattt
    10741 ctttggcttt ggtctatgat ttgaggaaaa acattaaaaa gctaacggta ttgtgcttat
    10801 tgattacagg gctctttctc ctgaccaagc atccactgga aaatgaaatc accatgctgg
    10861 atgtggggca aggagaaagt attttcctac gggatgtaac tgggaaaacc attctcatag
    10921 atgtaggtgg taaggcagaa tcttataaga aaatcaaaaa atggcaagaa aagatgacga
    10981 ccagcaatgc ccagcgaagc ttgataccct atctcaaaag tcgaggagta gctaagattg
    11041 accagctaat tttgactaac acggacaagg agcatgttgg agatttgtca gagatgacca
    11101 aggctttcca tgtaggggag attctagtat caaaagacag tctgaaacag aaggaatttg
    11161 tggcagaact acaggcgact caaacaaagg tgcgtagtat gatagtaggg gagaacttac
    11221 ccatttttgg aagtcagtta gaagttctat ctccaaggaa aatgggagat ggaggacacg
    11281 atgataccct agttctgtat gggaaattct tggataagca atttctcttc acgggaaatt
    11341 tggaggagaa aggagagaag gacttgctga agcactatcc agacttgaaa gtaaatgttt
    11401 tgaaagctag ccaacatggc aataaaaaat catcaagtcc agcctttcta gaaaaactca
    11461 aaccagagct tactcttatc tcagttggaa agagcaatcg aatgaaactc ccccatcagg
    11521 aaacattgac acgactggaa ggtatcaata gcaaagttta tcgaactgac cagcaaggag
    11581 ctatacgttt taaggggttg gatagttgga aaatcgaaag tgttcgatag gaaggataaa
    11641 tgttgtagat tagtgaaata aactaaaaat ttgttgcata ataatgataa aaacggcata
    11701 atgaaaacgt attcaatatt gaggatataa aatcattaaa aatcagcaaa agttgtttta
    11761 gttagttagt ttataatcta ttggtcttct tcagtccagt gtatctgctg tgacagtcac
    11821 taaaagttac aagtatgatt ggaatacggt tttggaatat agtaccaact atcacgacca
    11881 tcagtatgct tggattccgg aatggtctcg ttattacagc tattctgagt ataaagttgg
    11941 cggaggctgg aactacgctc gttatgaggt cataaactac tatactggag gttattaatc
    12001 cttaaagagt gagaaaagga gggctggata tgttaaatct tactcatgtt accttaaaaa
    12061 cgcgacaagt catcttgcaa gatgcggatt ttacctttaa aaagggtagg atttatggcc
    12121 ttcttgctat caatggctcg ggaaagacga cactattccg agctatgagc aagttgcttc
    12181 cccttagtag tggacacatc gcagttcctc cttctttgtt ttattatgag agcgttgaat
    12241 ggctggatgg aaacttaagt gggatggact accttcgtct cataaaaaac atctggaagt
    12301 cagacctaaa cttgagagat gaaatcgcct actgggaaat ggctgactat atcagtcttc
    12361 ccatccgcaa gtattcctta gggatgaagc aacgcttggt gattgctatg tattttctca
    12421 gtcaggccaa atgctggctc atggatgaga ttacaaatgg cttagacgag tattatcgac
    12481 agaagttttt tgataggcta gcacaaatcg atagacaaga acagctggtt cttttaagtt
    12541 cccactataa ggaagagttg gttgatatct gcgatagagt agtaaccatt catcaggggc
    12601 agatagaaga ggtttagttt atgaaagatg ttagtctatt tttattgaaa aaagttttca
    12661 aaagtcgttt aaactggatt atcttagctt tatttgtatc tgtactcggt gttacctttt
    12721 atttaaatag tcagactgca aactcacaca gcttggagag caggttggaa agtcgcattg
    12781 cagccaacga gagggctatc aatgaaaatg aagagaaact ctcccaaatg tctgatacca
    12841 gctcggagga ataccagttt gctaaaaata atttagacgt gcaaaaaaat cttttgacgc
    12901 gaaagacaga aattctaact ttactaaaag aagggcgctg gaaagaagcc tactatctgc
    12961 agtggaaaga tgaagagaag aattatgaat ttgtatcaaa tgacccgact gctagctctg
    13021 gcttaaaaat gggggttgac cgcgaacgga agatttacca agccctgtat cccttgaaca
    13081 taaaagcaca tactttggag tttccgaccc acgggattga tcagattgtc tggattttag
    13141 aggttatcat cccaagcttg tttgtggttg cgattatttt tatgctaaca caactatttg
    13201 cagaaagata tcaaaatcat ctggacacag ctcacttata tcctgtttca aaagtgacat
    13261 ttgcaatatc ctctcttgga gttggagtgg gatatgtaac tgtgctgttt atcggaatct
    13321 gtggcttttc ttttctagtg ggaagtctga taagtggttt tggacagtta gattatccct
    13381 acccaattta tagcttagtg aatcaagaag taactattgg gaaaatacaa gatgtattat
    13441 ttcctggctt gctcttagct ttcttagcct ttatcgtcat tgtggaagtt gtgtacttga
    13501 ttgcttactt tttcaagcaa aaaatgcctg tcctctttct ttcactcatt gggattgttg
    13561 gcttattgtt tggcatccaa acaattcagc ctcttcaaag gattgcacat ctgattccct
    13621 ttacttactt gcgttcagtg gagattttat ctggaagatt acctaagcag attgataatg
    13681 tcgatctaaa ttggagcatg ggaatggtct tacttccttg cctgattatc tttttgctat
    13741 tgggaattct atttattgaa agatggggaa gttcacagaa aaaaagaatt ttttaataga
    13801 ttctagcttt cctataggta gggaaaataa gtaaaaacta acatagagag ggaatcaact
    13861 tgattctctc tttttgattc gaaaaccaaa ccaaaataca aacacaaact tttcaaaaaa
    13921 taacttttta tcttgacaag agctagaaaa cttggtatca tataaaagtt gagaaaagca
    13981 gaagtgagag cttctcgcct tgtgacatta agttgcctgg ccctacggat gaaaagtttc
    14041 gaagaaacgc tatcataacg tgcgggcttg tatatttacg agtccgctat tgtttttctc
    14101 taataaaaca aaagaggtga aaaccatagc aaagcaagac ttattcatca atgatgagat
    14161 tcgtgtacgt gaagttcgct tgattggtct tgaaggagaa cagctaggca tcaagccact
    14221 cagtgaagcg caagctttgg ctgataacgc taatgttgac ctagtattga ttcaacccca
    14281 agccaaaccg cctgttgcaa aaattatgga ctacggtaag ttcaaatttg agtaccagaa
    14341 gaagcaaaaa gaacaacgta aaaaacaaag cgttgttact gtgaaagaag ttcgtctaag
    14401 tccaactatt gacaagggtg actttgatac aaaacttcgt aatgcacgta agttccttga
    14461 aaaaggaaat aaagttaagg tatctattcg ctttaagggt cgtatgatta cccataaaga
    14521 gattggtgca aaagttttag ccgagtttgc tgaagcaact caagatattg ccatcatcga
    14581 acaacgtgct aaaatggatg gacgtcaaat gttcatgcaa ttggcgccag cgactgacaa
    14641 aaaataattg tcagaaagtt aaataaagga gaaaaaatta tgccaaaaca aaaaacacac
    14701 cgcgcatcag ctaaacgttt caaacgtaca ggttctggtg gacttaaacg tttccgtgct
    14761 tacacttctc accgtttcca cggaaaaact aagaaacaac gtcgtcatct tcgtaaagca
    14821 tctatggtgc attcaggaga ttacaaacgt atcaaagcaa tgcttactcg cttgaaataa
    14881 tagcttgact gtaagtaaac aattctagga aatatttgga ggaaataaaa catggcacgt
    14941 gttaaaggtg gcgttgtatc acgcaaacgt cgtaaacgta ttcttaaatt agcaaaaggt
    15001 tactatggag ctaaacacat cttgttccgt actgcaaaag aacaagtaat gaactcttac
    15061 tactatgcat accgtgaccg tcgtcagaaa aaacgtgact tccgcaaatt gtggatcact
    15121 cgtatcaatg cggcagctcg tatgaacgga ctttcatact cacaattgat gcatggtttg
    15181 aaattggctg agatcgaagt taaccgtaaa atgcttgctg acttggctgt taacgatgca
    15241 gtagctttca cagctcttgc agatgcagct aaagcaaaac ttggtaaata acaactgatg
    15301 agactggaac aggattgtcc agtcttttta aaaataaagg agaaaaatat ggcttcaaaa
    15361 atgctacaca cttgcttacg agtagaaaat cttgaaaaat caatcgcatt ttatcaagat
    15421 gcttttggtt ttaaagaatt gcgtcgcaga gattttccag accatgcctt cacgattgtc
    15481 tatctaggtc ttgagggtga tgactatgag ttggagttga cttataacta cgatcacggt
    15541 ccgtatgtgg ttggtgatgg gtttgcccat atcgccctca gtacacctga tcttgaggca
    15601 cttcatcaag aacacagtgc aaaaggctat gaagtgactg agccaaatgg tctaccagga
    15661 actacaccaa actattactt tgtcaaagac cctgatggat ataaggtcga agtcattcgt
    15721 gaaaaataat ttcgtgcaag ttaagtagaa tattcgtttt ttacttgact ttttttagct
    15781 gaaagagtat actaataaaa attcaacctt taaggggagt ccagagagac tcacaaggtg
    15841 tcagataaaa gaatagtgca attttctaga ggagactttt tgagtgtgct ctcttgtgtt
    15901 gtacgatttt aactgaggcc ttgcactagc aaggtctttt ctttatctgg tccccttaaa
    15961 atttaaggag gaaaagttat gaatcccaca tgtaagaagc gtttgggtgt cattcggttg
    16021 gaaaccatga aggtggttgc acaagaggaa atcgcgccag caatctttga attagtccta
    16081 gaaggagaaa tggttgaagc catgcgagca ggccaatttc ttcatctgcg tgtaccagac
    16141 gatgcccatc ttttacgtcg tcctatttca atttcgtcta ttgacaaggc aaacaagcag
    16201 tgtcacctca tttatcggat tgagggagct gggactgcaa ttttttcaac cttaagtcag
    16261 ggagacactc ttgatgtgat ggggcctcag ggaaatggtt ttgacttgtc tgaccttgat
    16321 gagcagaatc aggttctcct tgttggtggt gggattggtg ttccaccctt gcttgaggtg
    16381 gccaaggaat tgcatgaacg tggagtgaaa gtagtgacag tcctcggttt tgctaataag
    16441 gatgctgtta ttttgaaaac ggaattggct cagtatggtc aggtctttgt aacgacagat
    16501 gatggttctt atggcatcaa gggaaatgtt tccgttgtta tcaatgattt agatagtcag
    16561 tttgatgctg tttactcgtg tggggctcca ggaatgatga agtatatcaa tcaaaccttt
    16621 gatgatcacc caagagccta tttatctctg gaatctcgta tggcttgtgg gatgggagct
    16681 tgctatgcct gtgttctaaa agtaccagaa agcgagacgg tcaaccaacg cgtctgtgaa
    16741 gatggtcctg ttttccgcac aggaacagtt gtattataag gagaaaatta tgactacaaa
    16801 tcgattacaa gtgtctctac ctggtttgga tttgaaaaat ccgattattc cagcatcagg
    16861 ttgttttggc tttggacaag agtatgccaa gtactatgat ttaaaccttt taggttctat
    16921 tatgatcaag gcgacaaccc ttgaaccacg ttttgggaac ccaactccaa gagtagcaga
    16981 gacgcctgct ggtatgctca atgcaattgg cttgcaaaat cctggtttag aggttgtttt
    17041 ggctgaaaag ctaccttggc tggaaagaga atatccaaat cttcctatta ttgccaatgt
    17101 agctggtttt tcaaaacaag agtatgcagc tgtttctcat gggatttcca aggcaactaa
    17161 tgtaaaagct atcgagctca atatttcttg tcccaatgtt gaccactgta atcatggact
    17221 tttgattggt caagatccag atttggctta tgatgtggtg aaagcagctg tggaagcctc
    17281 agaagtgcca gtttatgtca aattaacccc gagtgtgacc gatatcgtta ctgtcgcaaa
    17341 agctgcagaa gatgcgggag caagtggctt gaccatgatc aatactctgg ttggaatgcg
    17401 ctttgacctc aaaaccagaa aaccaatctt ggccaatgga acaggtggaa tgtcaggtcc
    17461 agcagtcttt ccagtagccc tcaaactcat ccgccaagta gcccaaacaa cagacctgcc
    17521 tatcattgga atggggggag tggattcggc tgaagctgcc ctagaaatgt atctggctgg
    17581 ggcatctgct atcggagttg gaacagctaa ctttaccaat ccttatgcct gccctgacat
    17641 catcgaaaat ttaccaaaag tcatggataa atacggtatt agcagtctgg aagaactccg
    17701 tcaggaagta aaagagtctc tgaggtaaac tgcaatcaat ctgttcttga ttttttatta
    17761 gtttgtaata tgaatttagg agaattttgg tacaataaaa taaataagaa cagaggaaga
    17821 aggttaatga agaaagtaag atttattttt ttagctctgc tatttttctt agctagtcca
    17881 gagggtgcaa tggctagtga tggtacttgg caaggaaaac agtatctgaa agaagatggc
    17941 agtcaagcag cgaatgagtg ggtttttgat actcattatc aatcttggtt ctatataaaa
    18001 gcagatgcta actatgctga aaatgaatgg ctaaagcaag gtgacgacta tttttacctc
    18061 aaatctggtg gctacatggc agccaatgaa tggatttggg ataaggaatc ttggttttat
    18121 ctcaaatttg atgggaaaat agctgaaaaa gaatgggtct acgattctca tagtcaagcc
    18181 tggtactact tcaaatccgg tggttacatg acagccaatg aatggatttg ggataaggaa
    18241 tcttggtttt acctcaagtc tgatggcaaa atggctgaaa aagaatgggt ctacgattct
    18301 catagtcaag cctggtacta cttcaaatct ggtggttaca tggcgaaaaa tgagacagta
    18361 gatggttatc agcttggaag cgatggtaaa tggcttggag gaaaagctac aaatgaaaat
    18421 gctgcttact atcaagtagt gcctgttaca gccaatgttt atgattcaga tggtgaaaag
    18481 ctttcctata tatcgcaagg tagtgtcgta tggctagata aggatagaaa aagtgatgac
    18541 aagcgcttgg ctattactat ttctggtttg tcaggctata tgaaaacaga agatttacaa
    18601 gcgctagatg ctagtaagga ctttatccct tattatgaga gtgatggcca ccgtttttat
    18661 cactatgtgg ctcagaatgc tagtatccca gtagcttctc atctttctga tatggaagta
    18721 ggcaagaaat attattcggc agatggcctg cattttgatg gttttaagct tgagaatccc
    18781 ttccttttca aagatttaac agaggctaca aactacagtg ctgaagaatt ggataaggta
    18841 tttagtttgc taaacattaa caatagcctt ttggagaaca agggcgctac ttttaaggaa
    18901 gccgaagaac attaccatat caatgctctt tatctccttg cccatagtgc cctagaaagt
    18961 aactggggaa gaagtaaaat tgccaaagat aagaataatt tctttggcat tacagcctat
    19021 gatacgaccc cttacctttc tgctaagaca tttgatgatg tggataaggg aattttaggt
    19081 gcaaccaagt ggattaagga aaattatatc gataggggaa gaactttcct tggaaacaag
    19141 gcttctggta tgaatgtgga atatgcttca gacccttatt ggggcgaaaa aattgctagt
    19201 gtgatgatga aaatcaatga gaagctaggt ggcaaagatt agtactataa gtgaatatga
    19261 tttgagtgaa tagtaagtta aaaatcctga tttcaagtaa aatcaggatt ttttcatgga
    19321 tgcaattttt ttggagtctg gtgtgacgcg gagggtcttt tgtcctgtgt aagtgacaaa
    19381 gccgggtttt ccaccagttg gtttattgag ttttttgact tcaatcatat ctacctgcac
    19441 cagattcgac aggcgccctt gagagaagta ggcagctaac tctgctgcgt ctgtcttgac
    19501 tgcatcagat gggtcaagat ttcctgagat gacaacatgg cttccaggaa tgtccttagc
    19561 atggaaccaa agttcctcct tgcgggccat tttaaaggtc aattcctcat tttgaagatt
    19621 gtttcgtccg acatagatga tggttttgcc atcgcttgct agatattgtt ctagtttttt
    19681 gcgtttctgg attttctccc gttgtcttct gcggataaaa cctgtttgaa tcaattcttc
    19741 acggatttca gcgatttctt ccagtccagc ttggttgagg acggtttcta cactttccag
    19801 atagagaata gtggctttgg tttcttcaat caaatcagtc aagtatttga cagcttcttt
    19861 gagtttctga taccgtttaa aatagcgttg ggcattctgg ttgggagtca gagccttatc
    19921 aagcgcaatc atgataggtt ggttggtata gtagttgtct aggataacct ggtcttggtc
    19981 gttaggcact tggtggagga aggttgtcag caattctcct ttttgacgaa attcttcagc
    20041 gttgtctgtc gccagtaact ctttttcctg ttttttgagt ttgtgtcggt ttttctgaag
    20101 ttcattttca acacgacgaa tcagttcact ggcctgctgt ttgacgcggt cgcgctcagc
    20161 cttatcctta tagtaggtgt ccaacaaatc agaaagattt gcaagaggct ctcccacctg
    20221 atttgcaaaa ggaactggac tgaaggaagt ctcagtcaag catggcttgg tttcttgatt
    20281 gaaaaaattt cggaaagcgg aaagtttttc actaaccagt atcctttcca attcatttgc
    20341 cgtatcgcgt cccagacctt gaaagaggct ttgaagattt tttgctgtta gttcttgggt
    20401 ttgcaggatt tcaaagagct tttcatcctt gatagtaaaa ggattgagag attttgtact
    20461 tggcggagcg atataggtcg atcctggaag taaggtgcgg tagctatttt gtgaaaagcc
    20521 gacgtgtttg ataacttcga ggattttatg actgctttta tcgaccagta gaatattact
    20581 gtgtttcccc ataatttcga taatcaaggt agcctggata tggtctccaa tctcgttttt
    20641 attggaaact gtaatttcca caatacggtc attttccact tgctcaatcg actcaatcag
    20701 ggccccctgc aaatactttc tcaaaaccat gataaaggta gaaggttggg ctggattttc
    20761 aaaagtcgtt tgggtcagct gaatgcgtcc aaaaactgga tgggcagaaa ggagcaggcg
    20821 atggctttgg cgattgctgc ggatttgcaa gaccaactct tgttcaaaag gctgattgat
    20881 tttctggatg cgaccattca ctaattcgct tcgcaattcc tcaactatgt ggtgtaaaaa
    20941 aaatccgtca aatgacatcg ttctctcctt gtgattgtat tccatagtat tatatcaaaa
    21001 aggtagaata aaatcatgga aatgtggtat aataaagcca agtaaagaga aacgagaagc
    21061 acatgtatat tgaaatggta gatgaaactg gtcaagtttc aaaagaaatg ttgcaacaaa
    21121 cccaagaaat tttggaattt gcagcccaaa aattaggaaa agaagacaag gagatggcag
    21181 tcacttttgt gaccaatgag cgtagtcatg aacttaatct ggggtaccgt gacaccgacc
    21241 gtccgacaga tgtcatcagc cttgagtata aaccagaatt ggaaattgcc tttgacgaag
    21301 aggatttgct tgaaaatcca gaattggcag agatgatgtc tgagtttgat gcctatattg
    21361 gggaattgtt catctctatc gataaggctc atgagcaggc cgaagaatat ggtcacagct
    21421 ttgagcgtga gatgggcttc ttggcagtac acggcttttt acatattaac ggctatgatc
    21481 actatactcc ggaagaagaa gcggagatgt tcggtttaca agaagaaatt ttgacagcct
    21541 atggactcac aagacaataa acgaaaatgg aaaaatcgtg acctgatatc cagtttagaa
    21601 tttgccatca cagggatttt tactgctatc aaggaagaac gcaatatgcg aaaacatgca
    21661 gtgacggctc tagtggtcat ccttgcaggt tttggttttc aggtgtcacg aatcgaatgg
    21721 ctctttctcc tattgagcat tttcttggtg gtagcctttg aaattatcaa ttctgctatc
    21781 gaaaatgtgg tggatttggc cagtcactat cacttttcca tgctggctaa aaatgccaag
    21841 gatatggcgg ctggcgcggt attagtggtt tctctttttg cagccttaac aggcgcattg
    21901 atttttctcc cacgaatctg ggatttatta ttttaaacag taagaggaaa ttatgacttt
    21961 taaatcaggc tttgtagcca ttttaggacg tcccaatgtt gggaagtcaa cctttttaaa
    22021 tcacgttatg gggcaaaaga ttgccatcat gagtgacaag gcgcagacaa cgcgcaataa
    22081 aatcatggga atttacacga ctgataagga gcaaattgtc tttatcgaca caccagggat
    22141 tcacaaacct aaaacagctc tcggagattt catggttgag tctgcctaca gtacccttcg
    22201 cgaagtggac actgttcttt tcatggtgcc tgctgatgaa gcgcgtggta agggggacga
    22261 tatgattatc gagcgtctca aggctgccaa ggttcctgtg attttggtgg tgaataaaat
    22321 cgataaggtc catccagacc agctcttgtc tcagattgat gacttccgta atcaaatgga
    22381 ctttaaggaa attgttccaa tctcagccct tcagggaaat aacgtgtctc gtctagtgga
    22441 tattttgagt gaaaatctgg atgaaggttt ccaatatttc ccgtctgatc aaatcacaga
    22501 ccatccagac cgtttcttag tttcagaaat ggttcgcgag aaagtcttgc acctaactcg
    22561 tgaagagatt ccgcattctg tagcagtagt tgttgactct atgaaacgag acgaagagac
    22621 agacaaggtt cacatccgtg caaccatcat ggtcgagcgc gatagccaaa aagggattat
    22681 catcggtaaa ggtggcgcta tgcttaagaa aatcggtagc atggcccgtc gtgatatcga
    22741 actcatgcta ggagacaagg tcttcctaga aacctgggtc aaggtcaaga aaaactggcg
    22801 cgataaaaag ctagatttgg ctgactttgg ctataatgaa agagaatact aagtagaggt
    22861 gggctcatgc ctgcttcttg tttttacaga aggaggactt atgcctgaat tacctgaggt
    22921 tgaaaccgtt tgtcgttgct tagaaaaatt gattatagga aagaagattt cgagtataga
    22981 aattcgctac cccaagatga ttaagacgga tttggaagag tttcaaaggg aattgcctag
    23041 tcagattatc gagtcaatgg gacgtcgtgg aaaatatttg cttttttatc tgacagacaa
    23101 ggttttgatt tcccatttgc ggatggaggg caagtatttt tactatccag accaaggacc
    23161 tgaacgcaag catgcccatg ttttctttca ttttgaagat ggtggcacgc ttgtttatga
    23221 ggatgttcgc aagtttggaa ccatggaact cttggtgcct gaccttttag acgcctactt
    23281 tatttctaaa aaattaggtc ctgaaccaag cgaacaagac tttgatttac aggtctttca
    23341 atctgccctt gccaagtcca aaaagcctat caaatcccat ctcctagacc agaccttggt
    23401 agctggactt ggcaatatct atgtggatga ggttctctgg cgagctcagg ttcatccagc
    23461 tagatcttcc cagactttga cagcagaaga agcgacagct attcatgacc aaaccattgc
    23521 tgttttgagc caggctgttg aaaaaggcgg ttccaccatt cggacctata ccaatgcctt
    23581 tggggaagat ggaagcatgc aggattttca tcaggtctat gataagactg gtcaaggatg
    23641 tgtacgctgt ggtaccatca ttgagaaaat tcaactaggt ggacgtggaa cccacttttg
    23701 tccaaactgt caaaggaggg actgatgggg aaaatcatcg gaatcactgg gggaattgcc
    23761 tctggtaagt caactgtgac aaattttcta agacagcaag gctttcaagt agtggatgcc
    23821 gacgcagtcg tccaccaact acagaaacct ggtggtcgtc tgtttgaggc tctagtacag
    23881 cacttttggc aagaaatcat tcttgaaaac ggagaactca atcgccctct cctagctagt
    23941 ctcatctttt caaatcctga tgaacgagaa tggtctaagc aaattcaagg ggagattatc
    24001 cgtgaggaac tggctacttt gagagaacag ttggctcaga cagaagagat tttcttcatg
    24061 gatattcccc tactttttga gcaggactac agcgattggt ttgctgagac ttggttggtc
    24121 tatgtggacc gagatgccca agtagaacgc ttaatgaaaa gggaccagtt gtccaaagat
    24181 gaagctgagt ctcgtctggc agcccagtgg cctttagaaa aaaagaaaga tttggccagc
    24241 caggttcttg ataataatgg caatcagaac cagcttctta atcaagtgca tatccttctt
    24301 gagggaggta ggcaagatga cagagattaa ctggaaggat aatctgcgca ttgcctggtt
    24361 tggtaatttt ctgacaggag ccagtatttc tttggttgta ccttttatgc ctatcttcgt
    24421 ggaaaatcta ggtgtaggga gtgagcaagt cgctttttat gcaggcttag caatttctgt
    24481 ctctgctatt tccgcggcgc tcttttctcc tatttggggt attcttgctg acaaatacgg
    24541 ccgaaaaccc atgatgattc gggcaggtct tgctatgact atcactatgg gaggcttggc
    24601 ctttgtccca aatatctatt ggttaatctt tcttcgttta ctaaacggtg tatttgcagg
    24661 ttttgttcct aatgcaacgg cactgatagc cagtcaggtt ccaaaggaga aatcaggctc
    24721 tgccttaggt actttgtcta caggcgtagt tgcaggtact ctaactggtc cctttattgg
    24781 tggctttatc gcagaattat ttggcattcg tacagttttc ttactggttg gtagttttct
    24841 atttttagct gctattttga ctatttgctt tatcaaggaa gattttcaac cagtagccaa
    24901 ggaaaaggct attccaacaa aggaattatt tacctcggtt aaatatccct atcttttgct
    24961 caatctcttt ttaaccagtt ttgtcatcca attttcagct caatcgattg gccctatttt
    25021 ggctctttat gtacgcgatt tagggcagac agagaatctt ctttttgtct ctggtttgat
    25081 tgtgtccagt atgggctttt ccagcatgat gagtgcagga gtcatgggca agctaggtga
    25141 caaggtgggc aatcatcgtc tcttggttgt cgcccagttt tattcagtca tcatctatct
    25201 cctctgtgcc aatgcctcta gcccccttca actaggactc tatcgtttcc tctttggatt
    25261 gggaaccggt gccttgattc ccggggttaa tgccctactc agcaaaatga ctcccaaagc
    25321 cggcatttcg agggtctttg ccttcaatca ggtattcttt tatctgggag gtgttgttgg
    25381 tcccatggca ggttctgcag tagcaggtca atttggctac catgctgtct tttatgcgac
    25441 gagcctttgt gttgccttta gttgtctctt taacctgatt caatttcgaa cattattaaa
    25501 agtaaaggaa atctagtgcg agtaaaaatt aatctcaaat gctcctcttg tggcagtatc
    25561 aattacctaa ccagtaaaaa ttcaaaaacc catccagaca agattgaggt tttaaagtat
    25621 tgtcccaagg aaagaaaagt aaccctacat cttgaatcta agtagatttt atggtaaaat
    25681 aatagggatt ttaaggagtt tgatatgtat aacctattat taaccatttt attagtatta
    25741 tctgttgtga ttgtgattgc aattttcatg caaccaacca aaaaccaatc cagcaatgta
    25801 tttgatgcca gttcaggtga tttgtttgaa cgtagtaaag ctcgcggttt tgaagctgta
    25861 atgcagcgtt tgacagggat tttagtcttt ttctggctag ccattgcctt agcattgacg
    25921 gtattatcaa gtagataaga aaataatggg caggactagg tctttgcctc tttttatttt
    25981 taaaggatgt ttgagaaggt tttacagtaa aagaaaatta aaaaatctag aaagaaaata
    26041 tgaaagatag aataaaagaa tatttacaag acaagggaaa ggtgactgtt aatgatttgg
    26101 ctcaggcttt gggaaaagac agttccaagg attttcgtga gttgattaaa accttgtcct
    26161 taatggaaag aaagcaccaa attcgttttg aagaagatgg tagtctgaca ttagaaatta
    26221 agaaaaaaca tgagattacc ctcaagggga tttttcatgc ccataaaaat ggctttggct
    26281 ttgttagtct ggaaggcgag gaggacgacc tttttgtagg gaaaaatgat gtcaactatg
    26341 ctattgatgg tgataccgtc gaggtagtga ttaagaaagt cgctgaccgc aataagggaa
    26401 cagcagcaga agccaaaatt attgatatcc tagaacacag tttgacaaca gttgtcgggc
    26461 aaatcgttct ggatcaggaa aaacctaagt atgctggcta tattcgttca aaaaatcaga
    26521 aaatcagtca accgatttat gttaagaaac cagccctaaa attagaagga acagaagttc
    26581 tcaaagtctt tatcgataaa tacccaagca agaaacatga tttctttgtc gcgagtgttc
    26641 tcgatgtagt gggacactca acggatgtcg gaattgatgt tcttgaggtc ttggaatcaa
    26701 tggacattgt atccgagttt ccagaagctg ttgttaagga agcagaaagt gtgcctgatg
    26761 ctccgtctca aaaggatatg gaaggtcgtc tggatctaag agatgaaatt acctttacca
    26821 ttgacggtgc ggatgccaag gacttggacg atgcagtgca tatcaaggct ctgaaaaatg
    26881 gcaatctgga gcttggggtt cacatcgcag atgtttctta ttatgtgacc gaggggtctg
    26941 cccttgacaa ggaagccctt aaccgtgcga cttctgttta cgtgacagac cgagtggtgc
    27001 caatgcttcc agaacgacta tcaaatggca tctgctctct caatccccaa gttgaccgcc
    27061 tgacccagtc tgctattatg gagattgata aacatggtcg tgtggtcaac tataccatta
    27121 cacaaacagt tatcaagacc agttttcgta tgacctatag cgatgtcaat gatatcctag
    27181 ctggcgatga agaaaagaga aaagaatatc ataaaattgt atcaagtatc gaactcatgg
    27241 ccaagcttca tgaaacttta gaaaacatgc gtgtgaaacg tggagctctc aattttgata
    27301 ccaatgaagc gaagatttta gtggataaac aaggtaagcc tgttgatatc gttcttcggc
    27361 agcgtggtat tgccgagcgg atgattgagt cttttatgtt gatggctaat gaaacagttg
    27421 ccgaacattt cagcaagttg gatttgcctt ttatctatcg aattcacgag gagcctaagg
    27481 ctgaaaaggt tcagaagttt attgattatg cttcgagttt tggcttgcgc atttatggaa
    27541 ctgccagtga gattagtcag gaggcacttc aagacatcat gcgtgctgtt gagggagaac
    27601 cttatgcaga tgtattgtcc atgatgcttc ttcgctctat gcagcaggct cgttattcgg
    27661 agcacaatca cggccactat ggactagctg ctgactatta tactcacttt accagtccaa
    27721 ttcgtcgtta tccagacctt cttgttcacc gtatgattcg ggattacggc cgttctaagg
    27781 aaatagcaga gcattttgaa caagtgattc cagagattgc gacccagtct tccaaccgtg
    27841 aacgtcgtgc catagaagct gagcgtgaag tcgaagccat gaaaaaggct gagtatatgg
    27901 aagaatacgt gggtgaagag tatgatgcag ttgtatcaag tattgtcaaa ttcggtctct
    27961 ttgtcgaatt gccaaacaca gttgaaggct tgattcacat cactaatcta cctgaatttt
    28021 atcatttcaa tgagcgtgat ttgactcttc gtggagaaaa atcaggtatc actttccgag
    28081 tgggtcagca gatccgtatc cgtgttgaaa gagcggataa aatgactgga gagattgatt
    28141 tttcattcgt acctagtgag tttgatgtga ttgaaaaagg cttgaaacag tctagtcata
    28201 gtggcagagg gcgtggttca aatcgtcgtt cggataagaa ggaagacaag agaaaatcag
    28261 gacgctcaaa tgataagcgt aagcattcac aaaaagacaa gaagaaaaaa ggaaagaaac
    28321 ctttttacaa ggaagtagct aagaaaggag ccaagcatgg caaagggcga gggaaaggtc
    28381 gtcgcacaaa ataaaaaggc acgccacgac tatacaatcg tagatacgct agaggcaggg
    28441 atggtcctga ctggaactga aatcaagagt gtacgagctg ctcgaattaa tatcaaggat
    28501 ggctttgctc aagtgaaaaa tggagaagtt tggctgagca atgttcatat cgcgccttac
    28561 gaagagggca atatctggaa ccaggaacca gaacgtcgtc gtaaactcct gctccataaa
    28621 aagcaaattc aaaaattgga acaagagacc aaagggacag gaatgacctt agttcccctt
    28681 aaggtctata taaaagatgg ctacgctaag cttcttttag gacttgccaa agggaagcat
    28741 gactatgaca aacgggagtc tatcaaacgt cgtgagcaaa atcgagatat cgcgcgtgtg
    28801 atgaaagctg ttaatcagcg ataaaaagag gaattgaaaa tggaaaaatt agttgcctat
    28861 aaacgcatgc ctttgtggaa taaacaaaca atgcctgaag atgttcagca aaagcacaat
    28921 acaaaagttg ggacttgggg gaaaattact gtcttgaagg gagctctcaa gtttattgaa
    28981 ttgacagaag aaggggaagt tctagctgaa cacctctttg aagcaggggc agacaatcca
    29041 atggcccaac ctcaagcctg gcaccgagtg gaagctgcca cagatgatgt ggaatggtac
    29101 ttggaatttt attgtaaacc tgaggattat tttgctaaaa aatacaatac caatcctgtt
    29161 cattcagagg tcctagaggc catgcagaca gtgaaacaag ggaaagcttt ggatttgggt
    29221 tgtggtcagg ggcgtaattc tctttttcta gcccagcaag attttgatgt gacggctgta
    29281 gatcaaaatg gactagctct tgaaatcttg caaagcattg tggagcagga agatttggac
    29341 atgcctgttg gcctttacga tatcaattca gctagcattg aacaagaata tgattttatc
    29401 gtttcaacag ttgttctcat gtttctacaa gcggaccgca ttccagctat tattcaaaat
    29461 atgcaggaga aaaccagtgt tggtggttac aaccttatcg tttgtgccat ggacacggag
    29521 gattatcctt gctcggttaa tttcccattc acctttaaag aaggagaact ggcagactat
    29581 tacaaggatt gggaattggt taagtacaat gaaaatccag gccatttgca ccgtcgcgat
    29641 gagaatggca atcgtattca actacgcttt gcgaccttac tagctaagaa aatcaagtaa
    29701 acacacatga agattaggaa ttttcctgat cttttttctt ttttacgaat gatatagaaa
    29761 aggagggaat tcatgtttgt tgcgagagat gctaggggag aattggtaaa tgtgttagag
    29821 gataaacttg agaagcaagc atacacctgc ccagcttgtg gaggccagct ccatttgcgt
    29881 caaggaccaa gtgtacggac gcattttgcc cataaatcct taaaagactg tgattttttc
    29941 tttgaaaatg aaagtccaga acacctggcc aataaggaat ccctctatca ctggttgaaa
    30001 aaagagacaa aggttcaatt agagtacccg ctttcagaac ttaaacagat tgcggatgta
    30061 tttgtaaatg gcaatctagc tctagaagtt cagtgtagtc ccttgcctca gaaagtcctt
    30121 aaagagcgaa gtgagggata tcgtagtcag ggttaccaag tactgtggtt gctgggtcaa
    30181 aaactgtggc tcaaggagcg tttgactcgt ctacagcaag gttttcttta tttcagtcaa
    30241 aacatgggct tttatgtttg ggaattagac aaggaaaaac aagttttaag actcaaatac
    30301 ctgatttacc aggatctccg cggtaaactc cattatcaaa tcaaggaatt ttcctatggt
    30361 caaggtagtt tattggaaat attgcgtctt ccctataaga aacaaaaaat atctcatttt
    30421 acagtttctg aggacaagga catctgtcgc tatatccggc aacaacttta ttatcaaaat
    30481 ctcttttgga tgaaagaaca agcagaagcc tatcaaaagg gagaaaatat cctgacttat
    30541 ggactgaaag aatggtatcc acaaattcga ccaatagtgg gcaaattttt ccagattgaa
    30601 caagacttga ctagctatta tcagcacttt tatacctatt accaaaaaaa tcctcaaaat
    30661 gattggcaaa agctttatcc accagccttt tatcagcaat atttcttgaa aaatatggta
    30721 gaatagaaag gatggaggaa tctaatggta ttacaaagaa atgaaataaa tgaaaaagat
    30781 acatgggatc tatcaacgat ctacccaact gaccaggctt gggaagaagt cttaaaagat
    30841 ttaacagaac aattggagac agtagcccag tatgaaggcc atctcttgga tagtgcggat
    30901 aacctactag aaatcactga attttctctt gaaatggaac gccagatgga gaagctttac
    30961 gtttatgctc atatgaagaa tgaccaggat acacgtgaag ctaagtatca agagtactat
    31021 gccaaggcca tgacattcta cagccagtta gaccaagcct tttcattcta tgagcctgaa
    31081 tttatggaga ttagcgaaaa gcagtatgct gactttttag aagctcaacc aaagctgcag
    31141 gtttatcaac actattttga caagctcttg caaggcaagg atcacgttct ctcacaacgt
    31201 gaagaagaat tattggctgg agctggagaa atctttggtt cagcaagtga aaccttcgct
    31261 atcttggaca atgcggatat tgtgttccct tatgtcctag acgatgatgg taaagaagtt
    31321 cagctatctc atgggattta cacacgtttg atggagtcta aaaaacgtga ggttcgccgt
    31381 ggtgcctatc aagctcttta tgcgacttac gaacaattcc aacacaccta tgccaaaacc
    31441 ttgcaaacca atgttaaggt gcaaaattac cgtgctaaag ttcgtaacta caagagtgct
    31501 cgtcatgcag ccctcgcagc gaattttgtt ccagaaagtg tttatgacaa tttggtagca
    31561 gcagttcgca agcatttgcc actcttacat cgctatcttg agcttcgttc aaaaatcttg
    31621 gggatttcag atctcaagat gtacgatgtc tacacaccgc tttcatctgt tgaatacagt
    31681 tttacctacc aagaagcctt gaaaaaagca gaagatgctt tggcagtctt gggtgaggat
    31741 tacttgagcc gtgttaaacg tgccttcagc gagcgttgga ttgatgttta cgaaaatcaa
    31801 ggcaagcgtt caggtgccta ctctggtggt tcttacgata ccaatgcctt tatgcttctc
    31861 aactggcagg acaatctgga caatctcttt actcttgttc atgaaacagg tcacagtatg
    31921 cattcaagct atactcgtga aactcagcct tatgtttacg gagattactc tatctttttg
    31981 gctgagattg cctcaactac caatgaaaat atcttgacgg agaaattatt ggaagaagtg
    32041 gaagacgacg caacacgctt tgctattctc aataacttcc tagatggttt ccgtggaaca
    32101 gttttccgcc aaactcaatt tgctgagttt gaacacgcca ttcaccaagc agatcaaaat
    32161 ggggaggtct tgacaagcga tttcctaaat aaactctacg cagacttgaa ccaagagtat
    32221 tatggtttga gtaaggaaga caatcctgaa atccaatacg agtgggctcg cattccacac
    32281 ttctactata actactatgt atatcaatat tcaactggct ttgcggccgc ctcagccttg
    32341 gctgaaaaaa ttgtccatgg tagtcaagaa gaccgtgacc gctatatcga ctacctcaag
    32401 gcaggtaagt cggactatcc acttaatgtc atgagaaaag ctggtgttga tatggagaag
    32461 gaagactacc tcaacgatgc ctttgcagtc tttgaacgcc gtttaaatga gtttgaagcc
    32521 cttgttgaaa aattaggatt ggcataaaat ggttgaatcg tatagtaaga atgctaacca
    32581 taacatgcgt cgtcctgtcg tcaaagaaga aattgtagac ttgatgcgtc agcgtcaaaa
    32641 gcaggtcaca ggtttcttga aagaattgga aaactttgcc cgcaaggaaa atattcctat
    32701 tattccccat gaaacggttg cttatttccg ttttcttatg gaaaccatgc agcctaaaaa
    32761 tattctggaa attgggacgg ctatcggttt ttcagctctc ttgatggctg aacatgcgcc
    32821 aaatgctaag attacaacta ttgatcgtaa tccagaaatg attggttttg ccaaggaaaa
    32881 ttttgcccag tttgacagtc gcaagcaaat cactctccta gagggagatg cggtggatgt
    32941 cttatctaca ctgacagagt cttatgattt cgtctttatg gattctgcca agtctaaata
    33001 catcgtcttt ctgccagaaa tcctcaaaca tttggaagtt ggtggtgtgg ttgtcttgga
    33061 tgatattttt caaggtggtg atgttgccaa ggatattatg gaagtccgtc gtggtcagcg
    33121 aaccatttat cgaggccttc aaaaattatt tgatgcaacc ttagacaatc cagaactcac
    33181 cgcaacatta gtgcctttag gagatggtat tctcatgctt cgtaaaaatg tagcagatgt
    33241 tcaactgtct gaaagcgaat gattttcaga aaaatttaag aaaaaatagt aaaatagata
    33301 gagtaacact tatctcaaag gagtagacat gaagaaaaaa ttattggcag gtgccatcac
    33361 actattatca gtagcaactt tagcagcttg ttcgaaaggg tcagaagggg cagaccttat
    33421 cagcatgaaa ggggatgtca tcacagaaca tcaattttat gagcaagtga aaaacaaccc
    33481 ttcagcccaa caagtcttgt taaatatgac catccaaaaa gtttttgaaa aacaatatgg
    33541 ctcagagctt gatgataaag aggttgatga tactattgcc gaagaaaaaa aacaatatgg
    33601 cgaaaactac caacgtgtct tgtcacaagc aggtatgact cttgaaacac gtaaagctca
    33661 aattcgtaca agtaaattag ttgagttggc agttaagaag gtagcagaag ctgaattgac
    33721 agatgaagcc tataagaaag cctttgatga gtacactcca gatgtaacgg ctcaaatcat
    33781 ccgtcttaat aatgaagata aggccaaaga agttctcgaa aaagccaagg cagaaggtgc
    33841 tgattttgct caattagcca aagataattc aactgatgaa aaaacaaaag aaaatggtgg
    33901 agaaattacc tttgattctg cttcaacaga agtacctgag caagtcaaaa aagccgcttt
    33961 cgctttagat gtggatggtg tttctgatgt gattacagca actggcacac aagcctacag
    34021 tagccaatat tacattgtaa aactcactaa gaaaacagaa aaatcatcta atattgatga
    34081 ctacaaagaa aaattaaaaa ctgttatctt gactcaaaaa caaaatgatt caacatttgt
    34141 tcaaagcatt atcggaaaag aattgcaagc agccaatatc aaggttaagg accaagcctt
    34201 ccaaaatatc tttacccaat atatcggtgg tggagattca agctcaagca gtagtacatc
    34261 aaacgaatag tccaaatcaa tgagtcaggg aaaaaaactc gacttcagga aaaaatgaag
    34321 caaacattcc cacaataaaa cgcatagtac aaggtttgta ctgaccccaa aag
//
LOCUS       DAVBCG010000008        34204 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBCG010000008 DAVBCG010000000
VERSION     DAVBCG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.008"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(154..1140)
                     /locus_tag="VQ495_000327"
     CDS             complement(154..1140)
                     /locus_tag="VQ495_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2530339.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1150..1950)
                     /locus_tag="VQ495_000328"
     CDS             complement(1150..1950)
                     /locus_tag="VQ495_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2530340.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2203..3045)
                     /locus_tag="VQ495_000329"
     CDS             complement(2203..3045)
                     /locus_tag="VQ495_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2530341.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(3047..4339)
                     /locus_tag="VQ495_000330"
     CDS             complement(3047..4339)
                     /locus_tag="VQ495_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2530342.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4450..5721)
                     /locus_tag="VQ495_000331"
     CDS             complement(4450..5721)
                     /locus_tag="VQ495_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2530343.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6271..7788
                     /gene="malQ"
                     /locus_tag="VQ495_000332"
     CDS             6271..7788
                     /gene="malQ"
                     /locus_tag="VQ495_000332"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2530344.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7814..10072
                     /gene="glgP"
                     /locus_tag="VQ495_000333"
     CDS             7814..10072
                     /gene="glgP"
                     /locus_tag="VQ495_000333"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HET2530345.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLKSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11117..11689)
                     /locus_tag="VQ495_000334"
     CDS             complement(11117..11689)
                     /locus_tag="VQ495_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530346.1"
                     /translation="MDKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHVAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(12311..13159)
                     /locus_tag="VQ495_000335"
     CDS             complement(12311..13159)
                     /locus_tag="VQ495_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2530347.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13311..13589
                     /locus_tag="VQ495_000336"
     CDS             13311..13589
                     /locus_tag="VQ495_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530348.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13591..15654
                     /locus_tag="VQ495_000337"
     CDS             13591..15654
                     /locus_tag="VQ495_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2530349.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIEFIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIKLMKKSEENKKTKQRYFIKMADKVVKYNFFGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15732..16988)
                     /locus_tag="VQ495_000338"
     CDS             complement(15732..16988)
                     /locus_tag="VQ495_000338"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2530350.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKVFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17130..19595
                     /locus_tag="VQ495_000339"
     CDS             17130..19595
                     /locus_tag="VQ495_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2530351.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVAGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNLTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGALATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19660..20571
                     /locus_tag="VQ495_000340"
     CDS             19660..20571
                     /locus_tag="VQ495_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2530352.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20801..21499
                     /gene="dapD"
                     /locus_tag="VQ495_000341"
     CDS             20801..21499
                     /gene="dapD"
                     /locus_tag="VQ495_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2530353.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21567..22697
                     /locus_tag="VQ495_000342"
     CDS             21567..22697
                     /locus_tag="VQ495_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2530354.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22709..23248
                     /locus_tag="VQ495_000343"
     CDS             22709..23248
                     /locus_tag="VQ495_000343"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2530355.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23232..23909
                     /locus_tag="VQ495_000344"
     CDS             23232..23909
                     /locus_tag="VQ495_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2530356.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(23981..24880)
                     /gene="galU"
                     /locus_tag="VQ495_000345"
     CDS             complement(23981..24880)
                     /gene="galU"
                     /locus_tag="VQ495_000345"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2530357.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(24902..25918)
                     /locus_tag="VQ495_000346"
     CDS             complement(24902..25918)
                     /locus_tag="VQ495_000346"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2530358.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            26130..26509
                     /locus_tag="VQ495_000347"
                     /pseudo
     CDS             26130..26509
                     /locus_tag="VQ495_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(26689..27114)
                     /locus_tag="VQ495_000348"
     CDS             complement(26689..27114)
                     /locus_tag="VQ495_000348"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2530359.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(27182..27832)
                     /gene="phoU"
                     /locus_tag="VQ495_000349"
     CDS             complement(27182..27832)
                     /gene="phoU"
                     /locus_tag="VQ495_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2530360.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(27847..28599)
                     /gene="pstB"
                     /locus_tag="VQ495_000350"
     CDS             complement(27847..28599)
                     /gene="pstB"
                     /locus_tag="VQ495_000350"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2530361.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(28601..29416)
                     /gene="pstA"
                     /locus_tag="VQ495_000351"
     CDS             complement(28601..29416)
                     /gene="pstA"
                     /locus_tag="VQ495_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2530362.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(29409..30272)
                     /gene="pstC"
                     /locus_tag="VQ495_000352"
     CDS             complement(29409..30272)
                     /gene="pstC"
                     /locus_tag="VQ495_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2530363.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(30390..31265)
                     /locus_tag="VQ495_000353"
     CDS             complement(30390..31265)
                     /locus_tag="VQ495_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2530364.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(31432..32763)
                     /locus_tag="VQ495_000354"
     CDS             complement(31432..32763)
                     /locus_tag="VQ495_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2530365.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(32764..33471)
                     /locus_tag="VQ495_000355"
     CDS             complement(32764..33471)
                     /locus_tag="VQ495_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2530366.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(33555..34040)
                     /locus_tag="VQ495_000356"
     CDS             complement(33555..34040)
                     /locus_tag="VQ495_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2530367.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            34026..34204
                     /locus_tag="VQ495_000357"
                     /pseudo
     CDS             34026..34204
                     /locus_tag="VQ495_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        10078 a         6445 c         7338 g        10343 t
ORIGIN      
        1 tttgattttc attgagtata aaaagatctt gggaaaagac tcaatttcag tagaaaagga
       61 agtaaatctt cccacattaa aacgcatagt atcaagtttt ttcaatacct gatagtatgc
      121 gtttttgctt ttaacgactt tttgcccagt ctcttatttt tcgataattt tgtgggcgat
      181 caattgacgg taacaaattt gtttattgtt tttattatca ttaataatct ggagaatagt
      241 ttcaagggaa acacgaccaa gctctaaact attgatatcg acataggctg ccaagttgag
      301 cttgggatta accgagtcaa agctgagaac agggacatcc agctggtgtt tggcaatata
      361 gttacaaacg ccttcagcta ggaggctatc ggttgtgatg atagcatcaa tttgtggatc
      421 gtgcttgaat aatcgcttgc taaatttata gcccttttct tccagaaact cgtcggcaaa
      481 gtagatgcga ttgttgtcag tggtaagttt gtaatgttta agcgcctgtt catagcctgt
      541 taaacggtct ttggtcacga agagcttttt acttcctccg ataaaggcaa tgcgtttgca
      601 gccttttttg atgaaatatt cagtcgcatc aaaaccagct tgaacattgt cgttgtcgac
      661 aagtgggatg aaaggagata gagatttacc taagataagg aaggggaact gttcttctgc
      721 gacgagtttt acgagagggt cttcttcttg ggcatagaga aagattagcc catctacacg
      781 cttgccgtag accatttgtg aaatagcgtt gagacgctcc ttctcatctt tccctgttgc
      841 tatctgaatg gcatagtggt tttcagatgc gacttgagag atgccacgta gaaccgatgg
      901 aaagaaagga ttctggtaga aggcgtctga gtcatcagga agaactaatc cgataacctg
      961 agtatagctg cttaccaagc tacgagcgtt gaggtttggg tggtagttga gttccttcat
     1021 agctttgcga acgcgttttt ttgtttcgtc gctaatggtt gatttatttt gaataacacg
     1081 ggttacggtt gaaggcgaaa caccagcagc cttggccacg tctttaatcg taacgggcat
     1141 aaaaatctcc tatttatggt aatttggatc acggaaatgt gttttataaa agatagtgac
     1201 cagatacaga acaaaaagaa tattttgtac agtaatcagg gttgtcatat tttggccaaa
     1261 taatcccaaa ataagtgtaa tcagagtcgg caatcctaaa cagttcaaga taaagtgata
     1321 gcactcttta aaggttctaa atgaaaagag gcgtgatttc ttagtgatat aaaggagaag
     1381 actagctcct agagagacga taaagaaatt caaaccaaag aggaagctcg caccgagaac
     1441 taggaagagg ctgatataga tacgattttg ttggtaccag tctttagaaa ttgcttgggt
     1501 caagctgtct ttgcttttga aactctcagt ctgaatggct cggtaagaga tgcgggtcag
     1561 ttccttgctt tccttgctga tgactagttc atttgtatcg aaatgcagtt gcaagtcctt
     1621 aggtaattcc ttgatttgac ttggaccaat cacaacagaa ggggcttgac tagctgttcc
     1681 agtataagtt aatgtgccat cgacaattgt agcatgttca gagagatcct ggacaacctc
     1741 atttgtcaga ggttcataga cattatcgat aaaagtttct agcggatagg tctcctggga
     1801 gctgttttgg atagcaatgg gtaccataga caagctgata aggaagatac tggtaaagag
     1861 aagttgaaac cagttgagct cgaaacgttt ggacaggggc ttacgaaacc cccaaatact
     1921 tgaaaaatag gaaaatggat atggaagcat aggtatcttt ctaaaaggtg ttttctatat
     1981 gagtattatt atatagagaa atgcgcttta tttcaagtat agggggtaaa ttgcttgcta
     2041 caaggatatt atttacagtg acaattgcta ataatcaatg aaaatcaaag tgcaaactag
     2101 ccacaggttg ctcaaagcac aactttgagg ttggagataa agctgacgtg gtttgaagag
     2161 attttcgaag agtataaaat gaaaaagggt ggcggggata aattatccct tgtcgccacc
     2221 acttgtaagt cctgaaacaa agttcttttg taggaagaag aagagaatac agattggaag
     2281 ggcgatgagg atagcacctg ctgagaagta ggcaatcttc aagtttttcg cattgttaac
     2341 gaaggtttgg agacctacgg caacagtaaa gtattctttc tcacgaagca agaaactaga
     2401 gaggatgtag tccccgaaag gtcccatgaa ggcccagaga gcttgtacgg caaccattgg
     2461 gcgaacaagt ggtagaacaa tttgccagaa gcggcggaag tgtcctgcac cgtctagttt
     2521 tgcagattcg tctaaagaca ttggcactgt atcgaagtag cctttcatga gccaagcatt
     2581 catcgggata ccaccaccaa cgtagaggaa gatgaggaac cagttgtggt taagggcgtt
     2641 caacataagc gccataacga agaaggctgt caaagcggcc atagttggca ccatttggat
     2701 gatcaagaag aagaccaaac tttgtttacg agccaagaag ttgtaacggc tgtaggcata
     2761 accagcaagt acgatgatac ttgtttgaac agccatggta attaaggcga taatcaaagt
     2821 gttgaggtac caagtaccgt acaaggtttc agtgaagagg cctttaaagt tatcaaaatt
     2881 gaggtcgata ttagtatcta gtttaaaggc tgagacgtta cctgctttaa aggctgacat
     2941 aatggtaatc aacagtggat agataattac aattgataga ccaatcaggt aaaggtaagt
     3001 aaggctttga gtcagtctac gtttgagttt aattgagtta ttcatcttag acgtcctcca
     3061 tatcaaatgc gtgtagtttc ttgaatgcga tcatagagat tgagatgaca atgatagaga
     3121 taatcaaggt aacagctgcc gccattgagt attgaggaga tgtacctgtt gtcaaacggt
     3181 agatccatga gatcaagata tcggttgaac cagctccacc tccgacacta ccaggtcctc
     3241 caccattgaa gaggtacatg atagagaagt tgttaaagtt gaaggtgtat tggctaatca
     3301 aagtaggtgc cgcaacagcc aaaatcattg ggaaagtgat gttgcggaat ttttgccaag
     3361 cgttggcacc gtcaatataa gctgcttcgt aaaggtcgtt aggaatagat tgcaagatac
     3421 ccaaagtcaa aacgtagatg tatgggaatc cgagccaacc ttgcatcata atcaaggcaa
     3481 tcttagtcca agttgggtct gttttccaag gaataagagc tccatcaagg aaaggaagga
     3541 atttagccaa gattggcaat acttgagtgt tgatagcacc gacactatcg ttaaacatgt
     3601 ttgagaatgt caagatagtg atgaaggctg ggacagccca aggaagaagg aaaataacac
     3661 caaagatacg ttttcctttg ataaatggtt ggttggcaat gatagctgtg aagataccaa
     3721 ttacgatttg taaagtagaa gctgccaaag cccaaatgat agtccaagaa agaacagaac
     3781 caaaggcaga acggaaggta ctcaagctcc aaatgtttgt aaagttggtc aaaccaaccc
     3841 agtccaacaa cttgtttggt ggcaagtgtt ggaagtcgta gttggtaaag gcgatcatca
     3901 aggttacgat aactgggaag ataatcgcga aggtcatggc aacataagat ggaatgatca
     3961 agaggtaagg gaagccattt tcataaatcc ctttgatcat gtctttgagt gtgcgtggaa
     4021 ctggaattcc attgttaatg cgttttgcaa tcgtatgtgc atctttgata tttgagaaat
     4081 aaaagagtac ataaacgatt acaaagatta gatggaaggc accacgaatc agcataaaga
     4141 gggaattatc acgacctggt ttgtcaccaa gagtgatgag gttgctcaat tcaggggttg
     4201 caagtgctag gaagtaaagg acaaatacga tggttacacc aaggaagata aaacctttgg
     4261 ctttttgttt attgtaaatc tgtcctaacc caggaatgat agacagcagg gctgctttac
     4321 taggttgttg cttttccata ttaactcctt tcataggata ctactttttt ataagaaagc
     4381 taaattattt gtgtctctat tgataaattc aaagggggat ttgatttcca ccccccttga
     4441 acaaattttt tattcaccaa atttttgttt gattgtttct ttgatcaatg ttacagcatc
     4501 gttagcagct gttttagcat ctttttgacc acttacagca tcaaagagca tatttttcgc
     4561 tggatcccaa actgcagaca tttgagagat gtttggcagt ggttgagtgt tcttgaactg
     4621 tttgataaca gctgttgtca actcatcgtt tttaccttca gcgtatgaac gagcctcagt
     4681 attagctggg atttcgttag tcttatcata taatactttt tgttgttcag ttgcaacaag
     4741 gaagtctaca aatttttgag aagcttcaag gttcttaacg gcttgaggaa tgacccaagc
     4801 tttaccacca ccgaatgcag catattcttt tccatttgga agagttggga tagttgcaac
     4861 tccgtagttt actttagcat ctttaaaggc ttgagctttc caaggtccgt cgatgatagc
     4921 agctgtttta ccttcttgga attgagtttg gattaagttt ccagcacctt ctgtatcttg
     4981 cataccttta ggccattttt cgtaccaaga tttagcgtag ttgatacctg cgatagaacc
     5041 gtcgtttgca agaccgatgt ctttagcgtc tttaccgttt tggccaaaga cgtaagcacc
     5101 gttaccggca agaagtccat atgtatagta gaagtttgtc cagtcagcta ggaaggcagt
     5161 agttttacca tcttcaccag cgaatgcgta tttgctatct ttagcaaggt tttccaagtc
     5221 agcaaatgtt tttggagcat ctttcaccaa gtctttgttg tagtacataa caagtgactc
     5281 gataacggca ggagcaccgt aaactttacc attagcagct gttacaagag atttagttgt
     5341 gtcgtctgtt ttagcaccat cgctcaattt cacttctgaa agttgtccgt cagaaccaag
     5401 gctacctaca cggtcgtatg gagccatcat aacatcaggg acattaccag attggttgtc
     5461 aagagaaagt ttatcaagac ctcctagagc atcaccagtt ttaagagtga cttttactcc
     5521 agcttctttt tcataagctt tagcaacctc ttcaatatag ctcttatatc cctcgtctac
     5581 atatacagtg agttctttga cttcagatga accagaatca gcaggcttat cagcagtttt
     5641 gcttccgcaa gctaccaaaa gcaagctagc aagtgtagca gttccaagca ccgcagtgct
     5701 cttcataaat ttagatgaca tagtgtattc ctcccaaaga atagcaagtt ttattgataa
     5761 ggaaacgcaa acgttttcct ttatgagctt agtatagcac aaatagaaaa cggttgcaag
     5821 tattttttgt aaaaatttta aaaaattttt aagcttgatt tgatagcata attttgcatt
     5881 ttaatagaag aaaagtatga aatagataag aatacagtat ttttaataaa aatatatgga
     5941 atctgtctaa agagggaaat caggaggggc tacctccctg tttctaatcc ctatctattt
     6001 tctatagacg tttgtgcttt gttgcatata aattcaagct ccctaaggag gttttgtact
     6061 ttgtagactg tctcgtgaga gtcaaacttt aggattagat agttttaagt tatctttgct
     6121 aaaatgagtt agtctcctta aaaataatca aaaaagtttt ttaaaaacgc ttgcaattat
     6181 gcttgaaaag gagtatactt ataagtaacg caaacgtttg cgtctgcaaa atacgcaacg
     6241 ttccattatt ttaacacacg aggtgctatt atgaaaaaac gtcaaagtgg tgtgttgatg
     6301 cacatctctt ctcttccagg agcttacgga atcggatcat ttggtcaaag tgcttacgac
     6361 ttcgttgatt tcttggtccg tacaaaacaa cgttactggc aaatccttcc attaggagca
     6421 actagttacg gggattctcc ttaccaatct ttctcagcct tcgcaggaaa cactcatttt
     6481 atcgatttag atatcttggt ggagcaaggt ttgttgggag cgagtgacct tgaaggagtt
     6541 gactttggta gcgatgcgtc tgaagttgac tatgctaaaa tctactatgc acgtcgtcct
     6601 cttttagaaa aagcggtgaa acgtttcttt gaagtcggag atgttaaaga ttttgagaaa
     6661 tttgctcaag acaaccaatc atggcttgag ctctttgctg agtatatggc tatcaaagag
     6721 cattttgaca atcttgcttg gactgaatgg ccagatgcag atgctcgtgc tcgtaaagct
     6781 tcagcacttg aaagctatcg tgagcaattg gcagacaagt tggtttacca ccgtgtgact
     6841 caatacttct tcttccaaca atggttgaaa ttgaaagctt acgctaacga caaccacatt
     6901 gaaatcgttg gggacatgcc aatctacgta gcggaagatt caagtgatat gtgggcaaat
     6961 ccacatctct tcaaaacaga tgtcaatggt aaggctactt gtatcgcagg atgcccacca
     7021 gatgagtttt ctgtaactgg tcagctttgg ggtaacccaa tctatgactg ggaagcaatg
     7081 gacaaagacg gctacaaatg gtggattgaa cgcttgcgtg aaagcttcaa aatctacgat
     7141 atcgttcgta tcgaccactt ccgtggcttc gaatcttatt gggaaatccc tgctggttcc
     7201 gatacagcag cacctggtga gtgggtgaaa ggtccaggct acaagctttt tgcagccgtt
     7261 aaggaagaac ttggtgagct aaacatcatc gcagaagacc ttggcttcat gacagatgaa
     7321 gtgatcgaat tacgtgaacg tactggcttc ccaggaatga agattcttca atttgccttc
     7381 aacccagaag acgaaagcat tgatagccca cacttggcac ctgctaactc agttatgtac
     7441 acaggaacac acgataacaa tacggttctt ggttggtacc gtaatgagat tgatgatgcg
     7501 actcgtgagt acatggctcg ttacacgaac cgtaaagaat acgaaacagt ggtacacgct
     7561 atgcttcgta cagtattttc atcagttagc tttatggcaa ttgcaactat gcaagattta
     7621 ctagaattgg atgaggcagc tcgtatgaac ttcccatcta cccttggtgg aaactggtct
     7681 tggcgtatga ctgaagatca attgacacca gctgtcgagg aaggtttgct tgacttgaca
     7741 acaatttatc gccgaattaa tgaaaatttg gtagatttaa agaaataaga caataatcag
     7801 gagacaacta aacatgttat cactacaaga atttgtacaa aatcgttaca ataaaaccat
     7861 tgcagaatgt agcaatgaag agctttacct tgctcttctt aactacagca agcttgcaag
     7921 cagccaaaaa ccagtcaaca ctggtaagaa aaaagtttac tacatctcag ctgagttctt
     7981 gattggtaaa ctcttgtcaa acaacttgat taaccttggt ctttacgacg atgttaaaaa
     8041 agaacttgca gctgcaggta aagacttgat cgaagttgaa gaagttgaat tggaaccatc
     8101 tcttggtaat ggtggtttgg gacgtttggc tgcctgcttt atcgactcaa ttgctactct
     8161 tggtttgaat ggtgacggtg ttggtcttaa ctaccacttt ggtcttttcc aacaagttct
     8221 taaaaacaac caacaagaaa caattccaaa tgcatggttg acagagcaaa actggttggt
     8281 tcgctcaagc cgtagctacc aagtaccatt tgcagacttt actttgaaat caactcttta
     8341 cgatattgat gttactggtt atgaaacagc gactaaaaac cgcttgcgtt tgtttgactt
     8401 ggattcagtt gattcttcta ttattaaaga tggtatcaac tttgacaaga cagatatcgc
     8461 tcgcaacttg actctcttcc tttacccaga tgatagtgac cgtcaaggtg aattgctccg
     8521 tatcttccaa caatacttca tggtttcaaa cggtgcgcaa ttgatcatcg acgaagcaat
     8581 cgaaaaagga agcaacttgc atgaccttgc tgactacgca gttgtccaaa tcaacgatac
     8641 tcacccatca atggtgattc ctgaattgat tcgtcttttg actgcacgtg gtatcgagct
     8701 tgacgaagca atctcaattg ttcgtagcat gactgcctac actaaccaca caatccttgc
     8761 tgaggcgctt gaaaaatggc ctcttgaatt cttgcaagaa gtggttcctc acttggtacc
     8821 aatcatcgaa gaattggacc gtcgtgtgaa ggcagagtac aaagatccag ctgttcaaat
     8881 catcgatgag agcggacgtg ttcacatggc tcacatggat atccactacg gatacagtgt
     8941 taacggggtt gcagcacttc atactgaaat cttgaaaaat tctgagttga aagccttcta
     9001 cgacctttac ccagaaaagt tcaacaacaa aacaaacggt atcactttcc gtcgttggct
     9061 tatgcatgct aacccaagat tgtctcacta cttggatgag attcttggag atggttggca
     9121 ccatgaagca gatgagcttg aaaaactgtt gtcttatgaa gacaaagcag ctgtcaaaga
     9181 aaaattggaa agcatcaagg ctcacaacaa acgtaaattg gctcgtcact tgaaagaaca
     9241 ccaaggtgtg gaaatcaatc caaattctat ctttgatatc caaatcaaac gtcttcacga
     9301 gtacaaacgc caacaaatga acgctttgta cgtgatccac aaataccttg acatcaaagc
     9361 tggtaacatc cctgctcgtc caatcacaat cttctttggt ggtaaagcag ctccagccta
     9421 cacaatcgct caagacatta tccatttaat cctttgcatg tcagaagtta ttgctaacga
     9481 tccagcagta gctccacact tgcaagtagt tatggttgaa aactacaacg ttactgcagc
     9541 aagtttcctt atcccagcat gtgatatctc agaacaaatc tcacttgctt ctaaagaagc
     9601 ttcaggtact ggtaacatga aattcatgtt gaacggagct ttgacacttg gtactatgga
     9661 cggtgctaac gtggaaatcg ctgagttggt tggagaagaa aacatctaca tcttcggtga
     9721 agattcagaa actgttatcg acctttacgc aaaagcagct tacaaatcaa gcgaattcta
     9781 cgctcgtgaa gctatcaaac cattggttga cttcatcgtt agtgatgcag ttcttgcagc
     9841 tggaaacaaa gagcgcttgg aacgtcttta caatgaattg atcaacaaag actggttcat
     9901 gactcttctt gatttggaag actacatcaa agtcaaagag caaatgcttg ctgactacga
     9961 agaccgtgac gcatggttgg ataaagtcat cgttaacatt tctaaagcag gattcttctc
    10021 atctgaccgt acaatcgctc agtataacga agacatctgg cacttgaact aatactcttc
    10081 gaaaatctct tcaaaccacg tcagctttat ctgcaacctc aaagcggtgc tttgagcaac
    10141 ctgcggctag cttcctagtt tgctctttga ttttcattga gtataagata caaatttata
    10201 ctaatacatc ttgtaaaaaa gcgagtttcg attgaaattc gcttttttaa atgctgtgga
    10261 tttgggtcaa tcttgtctaa aaatagggaa atcctagata cagtgaaggc tttaaatgct
    10321 ggtttttact gtcttcagcc ttatattttt tcgtagttgg ttacctcata tctcttgtat
    10381 tcgcttacat aaagtattat aatataattg taggaaagaa ggtgttttta tgatatacac
    10441 acttaaattg gtgttgttta ttacctttct tgtaataagc ttgttacctg ataagatttt
    10501 tggaaaaaat aaaaaaattt ggaaaatagt ttttgcaata ttgacggcag tggcagcatt
    10561 gtcatttatg tactaagtta ttttaagaat gtagggaaat aaaccctaca ttctttttag
    10621 ttttttctgt tttctaaatt ctatttatcc aagcgcttca acatttcttg cttcttcgct
    10681 tcaagttctg cacgcttttc ttcgatttcg gcatgttttt tctcgagttc agaacaactt
    10741 gcatcattgc taaattcttt tcgccatcag gagatagggt gagtcgacat gtctattact
    10801 cacccaaagc agtcctacaa agcaggaatt ttctgttact tttttggaaa tagtaacgtt
    10861 tatacagctt tgacacttcg tatcaaagcg ccaaacacac tccgagggat ttacagaaag
    10921 cagaaaagga atgatctggt ataagatcat tccttttctc tctttttctt taagtaatta
    10981 tatacaatgt acgacgaagt cgtcattgca atgctgatcc accacctaaa gggaacttta
    11041 aacaacattg ataagataaa gaatataaac aacgaaaata cgttataccc aattaatttt
    11101 attgtatatc tcatgattaa aagttaatcc ttccgttgtt aggaatggca tcatttttat
    11161 cccataattg tgctaaataa gtccccggtg ataataaatt catagcgaat tctaaagcaa
    11221 catcatttac aaaccaacta cctagatatc tagaaattgc tgaacgaata gcactttttg
    11281 ctacatgttt tccttttact ttaattagat ttgcaaggcc tgcagtagtt cctcctaatg
    11341 ctaaagctat tgcagtatct aatagagcac ccatttgatt aactgtaata ccttgccaag
    11401 ctgctctaaa tggagagtat gtaggtggga ttgtataatc gccttgtaat tgtcggttaa
    11461 ttacttcttt gatccattgt tgtgagacgt ctggatgaaa agattggatt tcgtttgcaa
    11521 gtgtattgat ttgttcttct gttagagaag tgacaggttg aagttccata tttgtttcaa
    11581 tttgtgatac ttgttcagaa gcgtatacag ctgaaacact tggaatcgct gatacaatta
    11641 acacaattga cgtcaaaaaa accgaaataa atttcattaa tttgtccatg agcttttctc
    11701 ctttttattt gtatctgctt acattttatc atatactgtt attatagtca aaaaaatatg
    11761 ctattatgtt aaaaaaatat ttttcaaaat ataaatggac ggatttattt tggattttat
    11821 ttgttatttt gacctgcctc tatattggta accatgattt gtttactctc aatcatcaag
    11881 aattctcttt tcgtggtagc gtttggggtc tggtactggc cttatatcac ttattgttca
    11941 ttgataagtt tgtaatttcg aatcgaaaat aaagattaga actatgattg actggctact
    12001 tttaatattg attttggagg aagattttgt ctctattatt tattatttta aatttattta
    12061 ttttgtataa gatctattct ttgaggcggg aaaaatcgaa atacttgatt tatacggcct
    12121 atatcatatt tggggtaaat gtagtatatg gtattcaatg gttattaaga gaactgattt
    12181 caactatttc cccttaatgt gtatggaaac tataacaaaa cgcataagat taaggtttgt
    12241 gtacctgttc ttatgcgttt ttgtcatatt gaagattttc ggtaagtttt atacctttgg
    12301 gaagtctagt ttagaatgct ttcccaacca aaatctctgc ttcgatggta atctctgtca
    12361 gttggctcca gtcaatcttg ctctggtcaa cgtgaaagag gagaggagtc atactgagta
    12421 gggcttggag ttgttctgct gtgatagtct tagtcagaga ggcagtttga ctagatagga
    12481 tggtaaagtg ttcctggaaa tgctccttga tatcttggtt agaatactcc ttgtttgtca
    12541 gctggtcctg tactctttga cggatttctt tgaggtgatt ttcagttggg ataaccttta
    12601 tcaagatacc gtctttggat aaaacgcgac gaaattctcc atagttggca ggtgagaaga
    12661 tatcaagcag aatatccatg ttagcgtctt ttataggaag tcgtgccaag tcgccaacaa
    12721 accaattgac tgcccagttg ggttcacttt tagcagcgat ttggactgaa tctttggaga
    12781 tgtcaaaggc atagaaagtt ttttcagagt gactttcttg tagtttgcga gaatagaatc
    12841 cttcaccaca accgatatcc aaaattgttg tggtagtttt tgagcttgca agcaagtcag
    12901 atacagcatc taagatagct tggtaaaagc cggcttctag gatttgttga cggttttgaa
    12961 aattttcctt gttatagtta gcagattgct tgatttgagg tgctagattg acatagccaa
    13021 atttcgccaa gtcaaaagaa tgacggttgc agcacttgaa attagtctct aacagagtca
    13081 gattttcttg acagatagga caggcaaagg cagtcgcaga agcaaaacgc tgaagtttgg
    13141 gcttgagatt tgtattcata gtttaagtgt atcaaaagag gcaaatgaaa gcaactttag
    13201 gaataagtag atgggagatt cagtagaaat aaaactaatt ctccaattta tagaatgaaa
    13261 ttgcttttat atctaaattg ctatataata atgataagga ggaaataagt atgttaaaag
    13321 aagtattaac cgttgcaaaa gttgcgaaaa aatcttcatt atttttgggt ggtgtcgcat
    13381 ttggtaccct tggtttgaaa atcttagcaa gtaaggaagc taaaaaaggt tattctaaag
    13441 ctttggctaa ggcttacaag ttgaaagacg agctagatgc atctgtttct gttgtgaagc
    13501 aacatggaga cgatgtcttg caagatgcca aatatttgta cgagcaagag aaaaaagaag
    13561 agcaattaga tagccttata ggtgaataat atgtctttta aagtgctaca tagaggatac
    13621 caacatatcc gactatcatc ttctttttca cttaccttgg atattcaaga ctatcttcgt
    13681 tccttggcga gagatgaaaa ggggattgag tttatccagt tttacatgga tcaacagcac
    13741 tttactctac gcataaaaga aggcttttct gtattagata atgcagaagc ctttttaaaa
    13801 agaattgata aagggaaagt ttctgagttg atgactcttc ccattcgtag agaagagagt
    13861 gcttattcta ttgtttcagg tgcagcggtt aagcgtgtac tttttcgtag ttttgtgccg
    13921 tatcctattc gctatatatg gacttgttat caggctttgg gttatattag agaagcctat
    13981 caaacactag cgcgtaagga actaacgatg gaagtcttgg actgttcggc tattttattg
    14041 tctttgttta tgaaccaatc caagacagct agcaatatca tgtttatgct tgatttgggg
    14101 aatcatttag atcagtggtc cttgaaaaaa actgcaacag atttagaaca gagtcttctt
    14161 gcaaaagaga gcgatgtatt cctagtacag ggcgatacgg ttgttagtat caagagttcc
    14221 gatgttcaaa taggagatgt cttgatctta tctcaaggaa atgaaattct gtttgatgga
    14281 caagtagttt caggtttagg tatggtcaac gaaagttcct tgacaggaga gagttttcca
    14341 gttgaaaaaa gagagtctga tttggtttgt gcaaatacag tattagaaac tggagagtta
    14401 cgcattcgtg taacagataa tcagatgaac agccgtattt tacagctgat taagttgatg
    14461 aagaaatctg aagaaaacaa gaaaacgaaa caacgctatt tcatcaagat ggcggataaa
    14521 gtcgtcaaat ataatttctt tggggctggg ctgacttacc tattgacagg ttctttttct
    14581 aaagctattt ctttcctatt ggtcgatttc tcctgcgctt tgaaaatctc tactcctgta
    14641 gcttatttga cagttatcaa ggaagggttg aaccgtgaaa tggtgattaa ggatggagat
    14701 gttctggaga aatatctgga agttgatact ttcttgtttg ataagacagg aacaatcaca
    14761 actagttatc ctatagttga aaaggtgtta ccttttggag actatagtga ggaagatatt
    14821 ctcagaatca gtgcctgtct tgaggaacac atttatcatc ctattgctaa tgccatcgtc
    14881 aagcaagctg agatagaggg aattgaacat gaggaaatgc atgggaaact ccaatatatc
    14941 gcaagcaagg ggatcaaatc tcatatagat ggccaaccag ttcttattgg gaattatgtc
    15001 ttgatgcagg atgagcagat tcatatcagt tcagaacaaa atgctttaat tgaagagtac
    15061 aagagtcact acaatctctt attcttggct tatcaaaatg aattgattgg aatgttctgc
    15121 attcatactc ctttgagaaa agaagcaaaa acagccttgg ataaacttaa ggcacagggg
    15181 aaaaaattga ttctggcaac aggagacacc ttgattagga cagaggaatt agtcaaagat
    15241 ttgccctttg atcaggtcta tacagacttg aaacctgatg ggaaatttga gttagtagag
    15301 aaactgcaga aagcaggtca cactattttg atggttggag atggattgaa tgactcagcg
    15361 gctttaactc tatcagatat cggtgtggtg atgaatgaga gtgcagatat ttctaagcag
    15421 atgagtgata tcttattgtt agataatcgt ttggatttct tccaagagtt ggattcgtta
    15481 tcatcatctt tgcaaacact catcaagaaa aatattcagg ataccgttgt cgtaaatagt
    15541 agtttgattg gctttggctt atttaattgg ttcagtcctt caaatctctc tatcttacat
    15601 aatctaacaa ccttgcgcat tgtcctgcgt agcctgtcta ttaaaaatag atagatgaga
    15661 gttattaaca gcaaaaagga gttacctttc tcgaggttaa ctcctttgtt tatagataaa
    15721 tgttgaatag tttagtaagt caatacaaag tattttttct tcccacgacg gataacagtc
    15781 agttcatttt ctaacttatc agcgtcactc aagacatagt caaggtcttg gatgcggtcg
    15841 ccgtttacgt agatggctcc gttttggacg tcttcacggg cttggcgttt tgagttaact
    15901 ataccagatg agacgagcag ttccacgata ttgttgtttt cgtctgcctg tacttggtag
    15961 tttggcacac cacgaagtcc ttgtttgagc tctttgacag aaaggttttt gatgtttcct
    16021 gcaaagagtt gctcagtgat gttaagtgct tctttgtagg cttcttctcc gtgaacaagt
    16081 gtaacaactt cacgagccaa gactttttga gccaagcgtt cgtgtggcgc tgcttcaaat
    16141 tgtttacgaa tatcttcaat ctcatcaagt gacaagaaag taaagacttt caagaagcga
    16201 acagcgtcag cgtccatcac gttcatccag aattggtaca tttcgtatgg agaagtcttt
    16261 tcgggattga gccagacggc atttccttct gatttaccaa atttcttacc agttgcatct
    16321 gtgattagtg gaacagtgat aacgtgacca gtcttgtccg ccttacgacg aagcaattcg
    16381 gtaccagctg tcatatttcc ccactggtca gaaccaccga tttgaagagt gacattatgg
    16441 tcttggttaa ggacgaagaa gtcgtaccct tgcatgattt ggtaagcgaa ctcagtgtaa
    16501 gaaattcctg tttcgatccg ttttttaaca gattccttac tcatcatgta gttgaccgtg
    16561 aagtattttc caatatcacg gaggaagtca atgaagctga tgctgccaaa ccagtcgtag
    16621 ttgttgacca tgacagcctt gttttcgcca ttttcaaagt caagaaaacg agaaagttgt
    16681 ccttggatag acttgaccca gccatctact gtgtcttttg tttggagact acgttcagca
    16741 tctttgaagg acggatctcc gatgagacct gtagcaccgc caacgagcgc ataaggtttg
    16801 tgacctgcta gttgcaagcg acgacttgtc aagattgcga caaggtggcc taggtgaagg
    16861 ctgtcagcag ttggatcgta gccagtataa taagaaactt gaccttcttc tagggcttta
    16921 cgcaaagctt cttcatcagt cgtttgaaat atcaaaccac gctcttttag ctcatcaaaa
    16981 atgtgcatgt gtcttttctc ctttttaaaa ttattgtttc tacctattgt atcacaaact
    17041 tgggcaatag cctagcagaa tagggaagaa agtggctatt ttattgaaaa tttccctttt
    17101 gtggtataat agatagagtg aggaaatcca tgcaaaatca attaaatgaa ttaaaacgaa
    17161 aaatgctgga atttttccag caaaaacaaa aaaataaaaa atcagctaga cctggcaaga
    17221 aaggttcaag taccaaaaaa tctaaaacct tagataagtc agccattttc ccagctattt
    17281 tactgagtat aaaagcctta tttaacttac tctttgtact cggttttcta ggaggaatgt
    17341 tgggagctgg gattgctttg ggatacggag tggccttatt tgacaaggtt cgggtgcctc
    17401 agacagaaga attggtgaat caggtcaagg acatctcttc tatttcagag attacctatt
    17461 cggacgggac ggtgattgct tccatagaga gtgatttgtt gcgcacttct atctcatctg
    17521 agcaaatttc ggaaaatctg aagaaggcta tcattgcgac agaagatgaa cactttaaag
    17581 aacataaggg tgtagtaccc aaggcggtga ttcgtgcgac cttggggaaa tttgtaggtt
    17641 tgggttcctc tagtgggggt tcaaccttga cccagcaact aattaaacag caggtggttg
    17701 gggatgcgcc gaccttggct cgtaaggcgg cagagattgt ggatgctctt gccttggaac
    17761 gcgccatgaa taaagatgag attttaacga cctatctcaa tgtggctccc tttggccgaa
    17821 ataataaggg acagaatatt gcaggggctc ggcaagcagc tgagggaatt ttcggtgtag
    17881 atgccagtca gttgactgtt cctcaagcag catttttagc aggacttcca cagagtccca
    17941 ttacttactc tccttatgaa aatactgggg agttgaagag tgatgaagac ctagaaattg
    18001 gcttaagacg ggctaaggca gttctttaca gtatgtatcg tacaggtgca ttaagcaaag
    18061 acgagtattc tcagtacaag gattatgacc ttaaacagga ctttttacca tcgggcacgg
    18121 ttgcaggaat ttcacgagac tatttatact ttacaacttt ggcagaagct caagaacgta
    18181 tgtatgatta tctagctcag agagacaatg tctccgctaa ggagttgaaa aatgaggcaa
    18241 ctcagaagtt ttatcgagat ttggcagcca aggaaattga aaatggtggt tataagatta
    18301 ctactaccat agatcagaaa attcattctg ccatgcaaag tgcggttgct gattatggct
    18361 atcttttaga cgatggaaca ggtcgtgtag aagtagggaa tgtcttgatg gacaaccaaa
    18421 caggtgctat tctaggcttt gtaggtggtc gtaattatca agaaaatcaa aataatcatg
    18481 cctttgatac caaacgttcg ccagcttcta ctaccaagcc cttgctggcc tacggtattg
    18541 ctattgacca gggcttgatg ggaagtgaaa cgattctatc taactatcca acaaactttg
    18601 ctaatggcaa tccgattatg tatgctaata gcaagggaac aggaatgatg accttgggag
    18661 aagctctgaa ctattcatgg aatatccctg cttactggac ctatcgtatg ctccgtgaaa
    18721 agggtgttga tgtcaagggt tatatggaaa agatgggtta cgagattcct gagtacggta
    18781 ttgagagctt gccaatgggt ggtggtattg aagtcacagt tgcccagcat accaatggct
    18841 atcagacctt agctaataat ggagtttatc atcagaagca tgtgatttca aagattgaag
    18901 cagcagatgg tagagtggtg tatgagtatc aggataaacc ggttcaagtc tattcaaaag
    18961 ctactgcgac gattatgcag ggattgctac gagaagttct atcctctcgt gtgacaacaa
    19021 ccttcaagtc taacctgact tctttaaatc ttactctggc taatgcagat tggattggga
    19081 agactggtac aaccaaccaa gacgaaaata tgtggctcat gctttcgaca cctagattaa
    19141 ccctaggtgg ctggattggg catgatgata atcattcatt gtcacgtaga gcaggttatt
    19201 ctaataactc taattacatg gctcatctgg taaatgcgat tcagcaagct tccccaagca
    19261 tttgggggaa cgagcgcttt gctttagatc ctagtgtagt gaaatcggaa gtcttgaaat
    19321 caacaggtca aaaaccaggg aaggtttctg ttgaaggaaa agaagtagag gtcacaggtt
    19381 cgactgttac cagctattgg gctaataagt caggagcgct agcgacaagt tatcgctttg
    19441 ctattggcgg aagtgatgcg gattatcaga atgcttggtc tagtattgtg gggagtctac
    19501 caactccatc cagctccagc agttcaagta gtagttctag cgatagcagt aactcaagta
    19561 ctacacgacc ttcttcttca agggcgagac gataattctc taaatgaagt ggccaatcaa
    19621 tggattgcca cttttttcct ttcgtgttac aataagggta tgaatcagta tcagaaaaag
    19681 attgttaacg gaaaaattta ttcgctccta tccggcttaa tatggggaat ctgtggaatt
    19741 ttaggagagt acttctttac tcattatcag gtgtcttcgg gctggattac ctctatgcgt
    19801 ttgacactgg cagggagtct tgtactcatt tggtctgcaa tacaattaaa atcgcaagtg
    19861 ctagatattt ggcgagacaa gaaaaattac ctgccctttt tagcttatgc tattttgggg
    19921 attttttcag tccagtattt tttctatctc tgtgtagaat actcaaatgc tacgacagca
    19981 actattttac agtttattag ccctgtcttt atcctctttt acaatcgctt ggtttatcaa
    20041 aaacgagcgt caaaaagcgc tgttttctat gttttggttg ccatgctggg tgtttgcttg
    20101 atggcgacaa agggagatct ctctcagtta tccatgacgc cgctagctct tataacaggt
    20161 ttgctgagtg ccatgggtgt tatgtttaat gttatcttgc cccaaccttt tgctaagcgt
    20221 tatggttttg ttcctacggt tgggtggggg atgattttgg caggtttgtt tagcaatgtc
    20281 ctctcgccgg tttatcagct ttcctttact cttgatattt ggagtatctt gatttgcctc
    20341 attatcgctt tctttggaac ggcttttgct tttttcattt ccatgaaggc tgtgtccttg
    20401 gtttctcctt tggtggttgc cgttatcagt gccagtgaac ctctctcttc tgctctcttg
    20461 agtgttttgt tcttaggatt agtagtggat tggtccctcc ttctagctat agccttgatt
    20521 attttaccca tgatattttt gtctatagaa gaagcgaaag aaagtagata aaaagtcttt
    20581 tcttaattct aaaagtgtgc tatactagaa tagtcaataa aacacgggga gatagttgtg
    20641 aagagtgttt ttaggttgta tcggtagggg cttgctttta ccgacagcac gttttatctc
    20701 acatccctaa aaaatcatac ctaaaaacag acaaaaaggt ttcaaatttg aggccttttt
    20761 tggtagaata ggtatcatta taacgaacca ggaggcacct atgactgcta caaaaatgaa
    20821 cgctcaagaa attatccaat ttatcgccaa tgctgaaaag aaaaccagtg tcaaagtaac
    20881 ctttgagggg caactcgcaa ctgctgtgcc tagctctgtt gtcaaactag gaaatgtcct
    20941 attcggagac tggaaggacg tggctccgct tcttgaaggt ttggtagaaa atcaagatta
    21001 tgttgtcgag caagatgctc gtaattctgc agttcctttg ctagataagc gtgctatcaa
    21061 cgctcgtatc gagccaggtg ctattatccg tgaccaggtg gaaattggtg acaatgctgt
    21121 tatcatgatg ggagctgtta tcaatatcgg tgctgaaatc ggtgctggaa ccatgattga
    21181 catgggtgcc atccttggtg gccgtgctat cgttggaaaa aatagccacg ttggtgcagg
    21241 tgcagttttg gcaggtgtga ttgagccagc tagtgctgaa ccagtctgtg tcggagacaa
    21301 tgttcttatc ggggctaatg cagtggttat cgaaggagtc caaatcggta gtggttcagt
    21361 tgtcgctgca ggagctatcg ttacccaaga tgtcccagaa aacgtggtag tagcaggtgt
    21421 tccagctcgt attatcaaag aaattgatgc ccaaactcaa caaaaaacag cgctagagga
    21481 tgcgcttcgt accttgtaat tgtaaaagta aaaaagaggc ggaacccttt ttccagcctc
    21541 tttctgctat atcggaggac agatagatgt tagatttgat tcagactaga cgagctttac
    21601 accagattcc agagattggc ttggaggagt tcaagactca ggcttatttg ctggatgtga
    21661 ttgagaaatt gactacgggc aaggattttg ttcaaattcg tacttggcgg acagggattt
    21721 tggtctacct gcagggaagt cagccggagc gaaccattgg ttggcggaca gatattgatg
    21781 gcctgcctat cgtcgaacaa acaggtctac cttttacttc tcaacaccaa ggtcgcatgc
    21841 atgcctgtgg tcatgatttt catatgacca ttgccttagg ctgtcttgag cgcgcccttg
    21901 aggagcaacc caagaataat ctgctctttc tatttcagcc tgctgaagaa aatgaagctg
    21961 gtggtatgct catgtatgag gacggtgctt ttggagattg gttgccaaac caattctatg
    22021 gtctccatgt tcgtccagat ttgaaagtcg gccagattgc gactaatact catacactct
    22081 ttgcaggaac ttgcgaggtg aagatccgtt tcaaaggtaa aggtggccac gcagcttttc
    22141 ctcatgaagc caatgacgcc ttggtggcgg ctagttactt tgtgacccaa gtgcagtcag
    22201 ttgttagccg caatgtcaac ccaatcgagg gagcagtggt gaccttcggc gtttttcaag
    22261 ctggaaccac caacaatgtc atcacagaca cagctttttt acatggaact attcgtgcct
    22321 tgacacagga catgagtctc ttggtgcaaa agagggtaaa aacagttgca gaaggggttg
    22381 cagctgcctt tgatatggaa gtcgaagtgg aactcaaaca aggtggatac ctacctgttg
    22441 agaacaatcc agccttggcg cgtgaactga tggacttctt tgacgagaaa gacggaatcg
    22501 agttgattga tatcgaacct gctatgacag gcgaggactt tggttatctc ctttcaaagg
    22561 ttgatggcgt gatgttttgg ctaggtatcg atagtcccta cgctcttcat caccctcaaa
    22621 tgagtcctaa ggaagaagtc ttagctattg gggtggctgc ggtgtctagt ttcctgaaaa
    22681 agaaggcagc agaatagagg gcttgtctat gaaatcggaa ttacgcaagc aagtcttgca
    22741 tgaaatgaag gctatatctc aagagcaaaa acaggctata gaccaagctt taaccgagcg
    22801 aattttacaa cacccctttt atcaagaagc caaggtcatc gcaacctacc tctctttttc
    22861 tcatgagttt caaacgcggg aactgattga gcaggcgctg aaggacggca agaaggtttt
    22921 gacacctaag acctatccca aggggcgcat ggattttgtg gtctatgatc cgcaacagtt
    22981 ggtaaaaact tcctttgcct tactggagcc acagggagat ttggaagtag tggatgcatc
    23041 tcagattgat ttgattcatg ttcctggcct ggcttttacg acggatggat atcggattgg
    23101 atatggtgga ggttattatg accgctatct ggaacatttt tctggtcgtg ctttgagtac
    23161 gatatatcct tgtcaaattc aggactttat acctgaaaac catgatattc ctgttcagga
    23221 ggtattaatt gatgaaggaa atctttgata gacgttaccc tgtgacgagt ttcttcctct
    23281 tagtgacggc cttggtattt ttactaatgt tggtcactgc aggcggaaac tttgacaggg
    23341 cagatacatt atttcgattt ggagccatgt atgggccagc tattcgcctc tttcccgagc
    23401 aggtttggcg tctcttgtct gccatttttg ttcatattgg gtgggaacat ttcattgtta
    23461 atatgctttc actttattat cttggaaggc aggtagagga gattttcggt tctaagcagt
    23521 ttttctttct ctatctttta tcaggaatga tgggcaatct ctttgttttt gtatttagtc
    23581 ctaaatcctt agcagcaggc gcctctacct ctctttatgg gctatttgcc gcgattattg
    23641 ttcttcgcta tgcaactcgc aatccttata ttcaacagct agggcaatcc tatctgacac
    23701 tttttgtagt taacattatt ggaagtgttc tgattccagg aatcagccta gcaggccata
    23761 tcggtggtgc agttggtggc gcatttctag cagttatctt tccagttaga ggagaaaaac
    23821 ggatgtataa taccagccag agattaggag cggtagtctt gttcgtagga ctcgccattt
    23881 tgcttttcta caagggaatg ggaatgtgac gaagttgaaa gaaaatttgt gtaatgaaaa
    23941 aagataaggc attttttgaa ccttatcttt ttattttatt ttattccttc tcagtcaatt
    24001 cttttccaag ttggatgagg taattcttca aatcatcttt gacttgtggg tgtttgaggg
    24061 cgtagtcgat ggatgttttc atgaagccaa acttgtctcc gacatcgtaa cgagccccat
    24121 tgaactcacg agcaaataca cgttgtgttt tattgagggt gtcgattgca tctgtcagct
    24181 gaatttcatt tcctgcacct ggagcttggt tttcgaggat ttggaaaatt tcaggcgtga
    24241 ggaggtagcg tccgataata gcaaggtcgc taggagcgtc ctctggagct ggtttttcaa
    24301 caaaggtttc aacactgtaa agaccatctt ttccttcgcc ttgcggagca ataaccccgt
    24361 aagcagatac ttcgtcatga gggactggca tgacagcgat agtagacgcg tgggtacgct
    24421 cgtagtcatc catgagttgt ttggtaagtg gaacagcctt ttcgtctgtg atatccatca
    24481 agtcatcacc aagcataacg acaaaaggtt catttccgac gaaagcctta gcttgcaaaa
    24541 cagcatctcc gagaccgcgt ggatgagttt ggcggataaa atgcagacgc atgtcagttg
    24601 ttttatcaac tagcttcaaa agatctgttt tccctttttc tttgaggtta tattccaatt
    24661 cgaagtttga atcaaagtgg tcctcaatag aacgttttga tttaccagtg acaactagaa
    24721 tatcttcaat acctgatttg agagcctctt ccacgataaa ctggatagtt ggtttgtcta
    24781 cgattggcaa catttctttg gcaagggcct tggttgctgg taaaaatcga gttcctagtc
    24841 cagcagcagg gatgactgcc tttctaactt ttgatgtcat aaaaatcctt tctatagagg
    24901 gttaagacca ctcattttct gctttaaatt cattgttcat gatgtcataa atggcatctt
    24961 tgatattggt tccgtgataa ataacttggt aaatagcctg tgtaatgggc atatagactc
    25021 caagttcttg ggctagttca taggctgctc gagtcgttga aattccttcg attaccatgc
    25081 ccatattagc ttctatatca gctagggatt ctcctcgtcc gagagcatct ccagctctcc
    25141 agttacgaga gtggatggaa gttcccgtta cgatcaaatc tcccacacca gataagccgc
    25201 tataggtcaa tggactggcc ccgagtgcta cccctaggcg ggtgatttct gctaaacctc
    25261 gagcgatgat ggctgcctta gcattatcac caaatccaag accatgtaaa gctccagcac
    25321 cgacagcaat aatattttta agagcaccag cagtttcaac cccgataaca tccgtattgg
    25381 tataaagtcg gaagtagtga ttactaaata gctcctgaac gtattgagct gtttgtaaat
    25441 ctttagaagc agcagtgatt aaagttaggt cacgcacaat ggtttcttcc gcatgactag
    25501 gccctgaaac aacgacaata tcactgcgga gctgttcagg aatttcttct tcaaggatag
    25561 ttgataatcg tttatggcta tcaggttcta atccctttga agcgtgcatg atgatagcct
    25621 tatggtccaa ggtttgtgca acttgttggg caacaagtcg tgtcactttt gttgggacaa
    25681 caaacaaaat cgcatccaca tctttcaatg tttctgctaa gtcggtgtag gcaatgatat
    25741 tttcatctag aacgacatct ttaaagtagt gcttgttagt atgataggta ttaatttcat
    25801 tgatttgctc gggaagattt ccccaaatac gtacctcgtg tccattgtca tttaagactt
    25861 gtgaaagggc agttccccaa gaaccaggcc ccaagacggc gacggtttgt ttttccatga
    25921 tttcctcctt gatagagttc tttccattat tctatcatat tctagaggat tttgcacttg
    25981 cattactggg gtatggtgga aaaatacaca aagaccgaga tgaagggata aactcttagt
    26041 ctttagttaa agtgagaaga aatctgatag atagtttgat tcagtatatg ctataaatag
    26101 aggtaaacaa ctcaggaggt tcttgtaggt tgcgagagtt tggcgaaaaa attaaaagat
    26161 tacgtttggc taaaaaaatc agtcgttcag aattttgtgg tgatgagtct gaattaagta
    26221 tccgtcaatt aattagaatt gaaaatggag aatccagacc aacactaaca aagttaaaat
    26281 atatcgctga acgtttgggg ggggggggaa gattacaagt tgatgctaag ttatatagag
    26341 ttggataagg aatacctaga attgaagtat ttcttgatga ggactcctac atacgaagat
    26401 gaaactatcg cccaaaagaa agagagtgtt tttgctaaga tttttgaaga gtattatgat
    26461 aggctacctg aggaagaaag atttatcatc ccaaattatt catatctagc actagcgaac
    26521 tacacagttc aaaaattacc agaaaagcta gttgaaatac tgtccttctg gtgattttgc
    26581 ttaatttctg atgttcgaga catgattgag gagatttgtc ttgatttttt cctaaaaaag
    26641 ggcagactcc tcccttggtt tgatgctcga tttttaaaat aaggtgactt attctgttag
    26701 gcatgttttc ccctgccctc tagctctata tggaacaggg agtatcagat ttatctggca
    26761 gattgtatca aataaggctt gaaactgttt tgaataggcg tatagacttg agaatttgag
    26821 aatatgtcgt ctagcagtag agacatattt tccggcaata tgaaggaaga gacgtcgaaa
    26881 acgcttgata gtcaaggact ttacttcatc accagctagc tgctttaaaa agaggtagag
    26941 attataagcc agacagccca tcatcatacg tacttcattc ttaatcatgg tcgaactatc
    27001 tgtcttatcc ccaaagaatc ctgctttcct ttctttgata aagttttcag cattccctcg
    27061 ttcacgatag aattgaaaca gctcctcact atctacactg cttaaattgt tcaaatgaac
    27121 ttttttacaa ccataaaaaa gagcacttaa tcgtactctt ctcttttaag gattagttga
    27181 attaattcaa atccactagt tctcctgttt ctaggtagac taggcgttca caaatgttag
    27241 caatgtaatc agcgaagcgt tccagatgtc ctatgatata aaggtattga gttccattgg
    27301 gaattgaggt ttcttggtct ttcataagtc caatgatttc ctttgataag gcataataat
    27361 attggtcaat ctgttcatct ttttgagcaa tactaatagc ttttgaggct tggtgcaaag
    27421 gaaaggcaac caataaatcg gctagcatgc tgagggataa tttacccatt tggtgtaact
    27481 gttcttcgtc aggggctagt tgattttctt ttagttgcaa aacagctttg gcaatgcctg
    27541 ccatatggtc tcccatacgt tcaaggtctg aacaagaaga catgatgcta atcacaaatc
    27601 gaaggtcaga cacttgtggc tgttgcaagg ccaacaaacg ggcacaggtc aattcgatag
    27661 cgctttgacc ttggttgata gcatgatcct tattgataat tagctctgcc atctccttgt
    27721 ctttggaggc taaggccagt aaggcttttg aagctgtttc aaggacaagt tgccctagtc
    27781 ctaaaaagga ttgttctaat tcatgcaatt ctaagtcaaa ttgatttctc ataggttttt
    27841 ccttccttat ccgaaccgtc ctgaaatata gtcttctgtg cgctgatctt ttggattggt
    27901 aaacacgtca acggtatctc caaattcgca aatttctcct gttaagaaaa aagcagtttt
    27961 atctgaaata cgtgaagctt gttgcatgtt atgggtaaca atgataatcg tataatcctt
    28021 ttttagttgc tgaatgaggt cttcaatttt taaagtggag atagggtcta aggctgaagt
    28081 cggctcatcc attaacagaa catcaggttc tactgctaaa gctcgcgcaa tgcaaaggcg
    28141 ttgctgctga ccgccagata aggacatggc actcttttta agatcatctt tgacttcttc
    28201 ccaaatggct gcccctttta aagatttctc cactaaggca tctaattgtt ttttgtctcg
    28261 aataccatgt gtccttggac cataagccac gttatcatag atagacatgg caaagggatt
    28321 aggctgttga aaaaccatcc ctacacgctt gcgtagctga ttaaggttga atttgctact
    28381 ataaatatct tgctcatcta agaggacttg gccttcaata tggcaagaag gaaccaaatc
    28441 gttcatccgg ttaagggttt ttagaaaagt tgatttgcca caaccagatg ggcctatcaa
    28501 ggcagtaatc tgtctttctg gtaattgaat cgaaatattt tttaaggctt gaaaatcccc
    28561 gtaaaataag tctaggtgtc tgactgaaaa tgttcccata ctaggaagct cctttcacaa
    28621 gttttcgaga taataagctt gatagagtat ttatcattaa aacagtaata atcaaaatca
    28681 caccggtagc ataggcttca ttgacatgta gcccctcact tgacagcata tacatatgca
    28741 gggctagaga acgacctgaa gacatgagac tacttggcgt attggtagag gtacctaatg
    28801 tatacatgag ggcagctgtc tcaccaacga tacggccaat agctagtatc actccagcta
    28861 aaatacctgg catggcaact ggtagaacaa ttctaaaaac agtccgtaac ttacctgccc
    28921 caagtccata acttgcttga cgcatgctat cactaacaga taaaagggct tcttctgttg
    28981 agcgaataat gactggcaac accatgatga ctgaggttaa gattcctgat aacagagagt
    29041 attgaaaacc taagaagact acaaagaaga gcatgccaaa cagaccaaaa acaatggaag
    29101 gaatcccaga caaggtatct gaggccaatc gcatgatttt aacacaaagg gaatcttttt
    29161 ttgtatattc cacaagataa aaaccagcaa aaatccctat gggcaaggct aaaagaagag
    29221 caccaaagac cagaataacg gtggaaataa tcgctggcat aagggaaatg ttctcagaag
    29281 tataagtcca agaaaagagg gatagactta gatgaggtaa gcctttgatg aggataaaac
    29341 caatgattaa aaagagagag ccaaaggtta aagctgaaaa acaataaacg agaagtttta
    29401 gcaggtattt actcataaga tgattttcct ttcaagtagg caaagtaggc attaatcaag
    29461 agaataagga aaaagagaac tgctgaggtt gcaataaggg cttccctatg ctgacctgat
    29521 gcgtaagcca tttccagaac aatattggtt gttaaggttc tggttcctga aaagagtcca
    29581 cttggaataa tcggctggtt gcctgccacc aaaataactg ccatggtttc acctactgcg
    29641 cgaccgattc ctaaaataac tgctgataaa ataccagatc tcgcagctgg caagatgaca
    29701 ctaaaaatac tccgttcatg actagctcct agagccaagc taccagaata atacgttttg
    29761 ggaactgttc ggatagcaga ttctgacaaa ctgataatgg ttggcaaaat cattattcct
    29821 aatagtaacg aagcggttag gacactcatg ccatttccta aaaaggttct aatccaaggc
    29881 accaataatt gtaggccgaa aaaaccataa acaatagatg gaatggccgc catcaagttg
    29941 atagctgatt ttaagaagcc atagacgggc tttggacaat aataaaccat aaacaccgat
    30001 gtcaagatgc ctgttggcac cccaatcaca atcgctccta aggtaattaa taaggaacca
    30061 acgatcattg gtaaaatacc atagcttgcc ggaatgttcg ttggcgacca atcactgcct
    30121 aataaaaaac gggcaaagcc gtagttagcc atgaaaggta agccattact aaaaataaag
    30181 aaacagatta gcaaaatagc tacaacagct actgttgcac tcatgaaaaa aattgcccta
    30241 aaaactgctt ctttgaaggc ttgttttgtc acatcttgtc ctttctagtg aagaaagtaa
    30301 gggagatacg acacctccct acttgccttc tttatcttat tgtacgatga aacgtctgca
    30361 tctctttaga gatttatgga gcaaacattt tatttaatct tgtcccaggt ggttaatttg
    30421 ccactaaaaa cgtctgcaag ttcagccata ctgacttggc ttgccttatt gtcattattg
    30481 accacaacag caataccgtc taaagcaata gcatcatggg tgagactctt accttcttca
    30541 ggagttaatt ccctagaaac cataccaata tcagcggttt tctccttaac agcggtaata
    30601 cctgctgaag acccattaga ggtaatatca atcgtaactt ctggattttc ttttttataa
    30661 gcttctgcta atttttccat taaagaagat actgaagtgg aacctacaac agacaacttg
    30721 cctgataagt gttggcttgt atattctgtg gtttcggttt tagcttcaat aaatttatta
    30781 tctgtgacca cttgttgacc ttgtttggag tggataaagc tgataaaatc ttgacctagc
    30841 ttggaaagat tagaagacca aacaatgttg aagggacgtt gaagagggta ttcaccatct
    30901 aaaactgtgt ctcgactagc cttgacacca tcaatctcta aagccttgac agatttcgtt
    30961 aaagatccca aggagatgta gccgatagca ttagcattcc cttgaactgc tgagagaaca
    31021 ccttctgtac tattttgaat cacagctgtt ttggcagtgt tgtcaatttt tttatcaccg
    31081 tcttttttga gaatccctgt gatttctgtg aaggcacccc gtgttccaga gccattttct
    31141 cgtgaaatca cctcaatcgt tcctgaagct gactgtttgg aagcagctga ctgattgcca
    31201 caggcaacaa gcccaaatcc tgataagcca atggctgcaa gagtaagcat ttttttgaat
    31261 ttcataataa tcacctttat ctctatgtat ttttcttgtg taggcttact acatttatag
    31321 tctaacaagt ctttgtaaag gtttatccct gattcatgta aagattgtgt aaagaatcaa
    31381 aaaaagccac ttttgaaaaa tggctgctcc taaaaatagc tttaaaaatt attagtcctg
    31441 tgcgaaagat tggttaggaa gaaaaatcgt gaagcaactg cctctgccaa gctgactcgt
    31501 caccgtgact tggccaccta ataattgact gagttctttg acaatggcaa ggccaagacc
    31561 agtgccacca gtttgtctgc ttcgaccttt attaactcgg taaaaacgtt caaaaatacg
    31621 atcctgctct aattgactaa taccaatccc tgtatctgat acagaaatct taatgccttc
    31681 gttcaccttt tgggtcttga cctcaatttt tcccccttgt tcagtgtaac ggatggcatt
    31741 ggataaaaga ttgagtaaga tttgggaaag taattgacta tctgatacga gggtgacatc
    31801 atctggcacc tgcaccttta gctgtaaatc cttcttcttg agctgaggtt gcaagctttg
    31861 agtcaaatcc tgtacaaatt ctgccaaaga aagggtcgtc cattgtatag gcatttgttg
    31921 agccttagat aaggtaagaa gatgctcaac aatatgctca agacgcaagc tttctttgta
    31981 aataatgtct agaaagtcat ccttgagcgc ttcttcttca gctgacatcc ccttaatggt
    32041 ttcagcaaag cccttaatcg aagtaactgg tgtcctcaat tcatgggagg catttgagac
    32101 aaaggctaaa tttaactttt cataagttct aatcgttgtt aaatcatata gcaagacgag
    32161 cacagcttcc acagattggg tggggctaaa aacgggaact gctgtcactt ctaaaatcaa
    32221 gtcaccctca tgaaacccac ttacttcttg ttttaacctt gttttttgat caaaggcttg
    32281 gtgaactaaa tttcgaatat ccatccgttt gaggtcatca agtgaactta tgtcgccgtc
    32341 cacatcggga aaataatgag gcagagagcg actggataat aacatctgac cttgagcgga
    32401 aactaaaaac gtccccatgg ttaggtgcga cagaagaacc tccattgttt cggctaggtc
    32461 cttgtattgc tgatcctgtt gggagacttt ggtttttagg gcagacacat actgagccaa
    32521 agactttaag tcttcttgcc ctttttctaa aaagtattca ctactggtca agagaggttg
    32581 gtgcaaggtc tcaaaagcaa cttcccattt ccaaaggcaa aagagccagt agccaccttg
    32641 tcccaaagaa agggctagaa gaaagagacc gatgccttta ctgatccaag ttaatgccat
    32701 ccctgcaatc agaatgaggc taacacttag attgactagc caaaattgaa ggtagcgttt
    32761 catctataac tccttgaact tataaccata accccgaatg gttcgaataa attgaggggc
    32821 tttaggattg tcttcaattt tttccctcaa cttaccaata tgaacgtcca ccaaacgtgt
    32881 ttcctgccca aagtcatacc cccagatacg ttccaaaaga cgctctctag tcagtgtcat
    32941 gttgggatgt ttcataagat agagcaagag ttcaaattct tttggggtca aactcagtaa
    33001 cttattcgcc ttgtagactt catgacgctc agggtatact ttcaaggtcc caaatagcca
    33061 agaatcgtca gcgatattat ctgaatcatc tccttcttgt tctcctttag ttcgcctgag
    33121 gacagccttg acacgcgcca gcaattctct agggctaaaa ggcttggtca ggtagtcatc
    33181 agcccctaat tccaaggcca aaaccttatc aaattcatca cttttcgcag aaaccatcat
    33241 aattggagtt ttgacgcctt tggctctcag ccgcttacaa acttccatgc catctaattg
    33301 tggtaacatg atatcaagca agataaaatc aaagggttct gtttctgcca aagctaaggc
    33361 cttccgtcca tttgttacca attgagtaga aaagccttcc ttacttaaat ggtagtcaag
    33421 caatttcaga atgtgttctt catcatccac taataagact tgttttgtca tctattatct
    33481 cctattggta acattataac acaattatca gaaatcctaa cattgctaaa tcagattaaa
    33541 tttgcttatc aagactagta tctggtcaaa cgctcaatca tctccttgtg ctctggatag
    33601 gtcgccagta gatctaccct ttcaaataat tcaaaatcct caaattcaaa accaggagca
    33661 acaagacaag aaaccagagc ataatcctta tcaactgttg atccccaaat agtgccctta
    33721 ggaacacagt agtgaagttg ttgccctttg gatatgtcca ggcctaaagt gactgcttcg
    33781 tagtgaccat ctgctgtaat catgtgaaca gtaagtgggg atcctgcatg aaaataccag
    33841 atttcatctg ctgtcaatcg gtgaaaatgt gaaggattcg tttcttctaa taagaaataa
    33901 atactggtat aaagcgccct tcccttacca gcaaggttta tagtgtctga agcttttttt
    33961 gtttgtctaa aatagccacc ttcaacatgg ggagctaact ctagagttct tatcaagtct
    34021 tctttatccg tcggagccaa tgggttgaag taactcttgt tcaaaggtgg tgttacgatt
    34081 tcaagaactc ctctcagctc tgaggacacg ataatgattg atgcgacgga agtacaaatc
    34141 aatcgcccta aaaaaagaat tagcgaatta ttctggtaaa aaaatgccac gctatgaagg
    34201 ctca
//
LOCUS       DAVBCG010000009        31978 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBCG010000009 DAVBCG010000000
VERSION     DAVBCG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.009"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            487..1530
                     /locus_tag="VQ495_000358"
     CDS             487..1530
                     /locus_tag="VQ495_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2530368.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1697..3784
                     /gene="topA"
                     /locus_tag="VQ495_000359"
     CDS             1697..3784
                     /gene="topA"
                     /locus_tag="VQ495_000359"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2530369.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            4005..4364
                     /locus_tag="VQ495_000360"
     CDS             4005..4364
                     /locus_tag="VQ495_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2530370.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4457..5089
                     /locus_tag="VQ495_000361"
     CDS             4457..5089
                     /locus_tag="VQ495_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2530371.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQLGVTQLHGTKVVF"
     gene            5133..5789
                     /locus_tag="VQ495_000362"
     CDS             5133..5789
                     /locus_tag="VQ495_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2530372.1"
                     /translation="MFEIFKSYQFNQEKAHAFGFVVNSGVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETDDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5808..6440
                     /locus_tag="VQ495_000363"
     CDS             5808..6440
                     /locus_tag="VQ495_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2530373.1"
                     /translation="MNFQLAKYSLLKKFSENIEFTTPGEYGAIFKYLIENVKTDRQII
                     CSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6468..6902
                     /locus_tag="VQ495_000364"
     CDS             6468..6902
                     /locus_tag="VQ495_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET2530374.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENACEMGRSVSYHDMPSV"
     gene            6914..7952
                     /gene="leuB"
                     /locus_tag="VQ495_000365"
                     /pseudo
     CDS             6914..7952
                     /gene="leuB"
                     /locus_tag="VQ495_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7949..8218
                     /locus_tag="VQ495_000366"
     CDS             7949..8218
                     /locus_tag="VQ495_000366"
                     /inference="COORDINATES: protein motif:HMM:NF018644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2530375.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRTWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8222..8581
                     /locus_tag="VQ495_000367"
     CDS             8222..8581
                     /locus_tag="VQ495_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2530376.1"
                     /translation="MAGSSREHAAWALANYGFKVVIAGTFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDHVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8743..9311
                     /locus_tag="VQ495_000368"
                     /pseudo
     CDS             8743..9311
                     /locus_tag="VQ495_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007122522.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9405..9470
                     /locus_tag="VQ495_000369"
                     /pseudo
     CDS             9405..9470
                     /locus_tag="VQ495_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9633..11564
                     /locus_tag="VQ495_000370"
     CDS             9633..11564
                     /locus_tag="VQ495_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2530377.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNESDENDDADY"
     gene            11551..12867
                     /locus_tag="VQ495_000371"
     CDS             11551..12867
                     /locus_tag="VQ495_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2530378.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            13042..14028
                     /locus_tag="VQ495_000372"
     CDS             13042..14028
                     /locus_tag="VQ495_000372"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2530379.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            14092..14163
                     /locus_tag="VQ495_000373"
     tRNA            14092..14163
                     /locus_tag="VQ495_000373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14125..14127,aa:Arg,seq:ccg)
     gene            14346..15044
                     /locus_tag="VQ495_000374"
     CDS             14346..15044
                     /locus_tag="VQ495_000374"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2530380.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            15035..18574
                     /gene="smc"
                     /locus_tag="VQ495_000375"
     CDS             15035..18574
                     /gene="smc"
                     /locus_tag="VQ495_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2530381.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18571..19365
                     /locus_tag="VQ495_000376"
     CDS             18571..19365
                     /locus_tag="VQ495_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2530382.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPAAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            19365..20183
                     /locus_tag="VQ495_000377"
     CDS             19365..20183
                     /locus_tag="VQ495_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2530383.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDDHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFADLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            20187..21464
                     /gene="ftsY"
                     /locus_tag="VQ495_000378"
     CDS             20187..21464
                     /gene="ftsY"
                     /locus_tag="VQ495_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2530384.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLATVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21510..22997)
                     /locus_tag="VQ495_000379"
     CDS             complement(21510..22997)
                     /locus_tag="VQ495_000379"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2530385.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(23146..23886)
                     /locus_tag="VQ495_000380"
     CDS             complement(23146..23886)
                     /locus_tag="VQ495_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2530386.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23886..26051)
                     /locus_tag="VQ495_000381"
     CDS             complement(23886..26051)
                     /locus_tag="VQ495_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2530387.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            26224..27177
                     /locus_tag="VQ495_000382"
     CDS             26224..27177
                     /locus_tag="VQ495_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2530388.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            27254..29242
                     /gene="uvrB"
                     /locus_tag="VQ495_000383"
     CDS             27254..29242
                     /gene="uvrB"
                     /locus_tag="VQ495_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2530389.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARIEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMTYNEEHDIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKVLD"
     gene            29252..29815
                     /locus_tag="VQ495_000384"
     CDS             29252..29815
                     /locus_tag="VQ495_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530390.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            29781..30269
                     /locus_tag="VQ495_000385"
     CDS             29781..30269
                     /locus_tag="VQ495_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530391.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            30343..30810
                     /locus_tag="VQ495_000386"
     CDS             30343..30810
                     /locus_tag="VQ495_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2530392.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(30807..31322)
                     /locus_tag="VQ495_000387"
     CDS             complement(30807..31322)
                     /locus_tag="VQ495_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2530393.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(31334..31861)
                     /locus_tag="VQ495_000388"
     CDS             complement(31334..31861)
                     /locus_tag="VQ495_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2530394.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT         9940 a         5646 c         7081 g         9311 t
ORIGIN      
        1 aaggtgcact gttatagatt caatacacta taatttagtg taattgagaa aggagaaatg
       61 attgtgattg atgttggcta ggttatgttc aatgattcct accgtctcaa atcttgtcag
      121 taaggaaaaa taaattcttc aaaagtagag attacaaggc ttgtttaaga aagaattcaa
      181 agaccttgac aaataaaaat aaaatggtta ttataaaaaa tggtctgaaa tagatgatga
      241 tacttttcga aaatcttttt acgtcagctc agctttgcct tgctgtgttt tgagcaagct
      301 acggttagct tccgagtttg attttcattt actagaaatg aaactgatga gagatatcag
      361 tagacatttg agtcaggata ttatggaaaa tgataaaaag agctcgtgag attggcatat
      421 cagactacta aagtattgag tttgttagga ttttagcgac tagttagctg ggaaaggaag
      481 atatttgtga caaataataa actgtattcg ttgatagaat ttagaaataa aatatatgaa
      541 gaattagaac tttccagaag tgatttagcg attttactat gtgccatgct tatcgcctct
      601 atcggattaa atatggattc gactcccgtg attattggag ccatgttaat ctctcctttg
      661 atgacaccta ttctgggagt ggggctctct ctagctatat ttgattttaa attgttaaga
      721 aaatctttta aaatattagc tattcaaatt cttgccagtt taatagcttc aacactttat
      781 ttttatcttt ctcccatttc gtatgctagt tcggaaattg ttgctagaac ctctccgact
      841 atttgggatg ttctcattgc ttttgtagga gggatagcag gtattattgg tgctaggaaa
      901 aaagagacca ataatattgt tcctggtgtt gctattgcaa ccgccttgat gcctcctctt
      961 tgtacagtag gttatgctat tgcttctgct aatctaaaat ttatcatagg ctcctcttac
     1021 ctattcctca tcaattgtag ctttattgtc attgcgactt atataggtgt taggttgatg
     1081 atggttaaga aacattattt taaagataat gaagaagact ctaaaatgcg taggattttg
     1141 cttctagttg ctgttttgct gatgattccg agtttcatct ctgcaacgac tttagtgaga
     1201 gaaacgttga aaaaagagtc ccttaagaaa tttatatcag agcagtttca ggggcataat
     1261 attttgaaaa aaacctattc taaaaagact cataccctaa agctaaccat ttcaggaaat
     1321 tatttgacag aagaagaact cgatatgatt tcaagtaaga gaggtgacta tggtttaagt
     1381 gatgtttctg ttcaagtttc acaattgtct gattcagaac aacttagtaa ggaagaactg
     1441 gtggagtatt tcttccagta tatcaaggat aaggaagcaa aagaaaagga aaaagctaat
     1501 aagttttata cagagtctga ggagcaataa tttcttgaga atagctggtt tctctcgtga
     1561 gtcttctatg tatatcaaag gaagactgag gttttaagta taaaactttt cttctattat
     1621 agtagctaag tcttgacagg gttcaaaaaa tggtttacac tttataaagt ttattacttt
     1681 gaaaaggtgt gatactgtgg ctacggcaac aaaaaagaaa aaatcaacag ttaaaaaaaa
     1741 tctagtcatc gtggagtcgc ctgctaaggc caagacgatt gaaaaatatc taggcagaaa
     1801 ctacaaggtt ttagccagtg tcgggcatat ccgtgatttg aagaaatcca gtatgtccgt
     1861 cgatattgaa aataattatg aaccgcaata tatcaatatc cgaggaaaag gccctcttat
     1921 caatgacttg aaaaaagaag ctaaaaaagc taataaagtt tttctcgcga gtgacccgga
     1981 ccgtgaagga gaagcgattt cttggcattt ggcccatatt ctcaacttgg atgaaaatga
     2041 tgccaaccgt gtggtcttca atgaaatcac caaggatgca gtcaaaaatg cttttaaaga
     2101 acctcgtaag atcgatatgg acttggtcga tgcccaacaa gctcgtcgga tcttggatcg
     2161 cttggtaggg tattcgattt cgcctatttt gtggaagaag gtcaagaagg gcttgtcagc
     2221 aggtcgcgtt cagtccattg cccttaaact catcattgac cgtgaaaatg aaatcaatgc
     2281 cttccagcca gaagaatact ggacagttga tgctgtcttt aaaaagggaa ccaaacaatt
     2341 tcatgcttcc ttctatggag tagatggtaa aaagatgaaa ctgaccagca atgacgaagt
     2401 caaggaagtc ttgtctcgtc tgactagtaa agacttttca gtagatcagg tggataagaa
     2461 agagcgcaag cgcaatgctc ctttacccta taccacttca tctatgcaga tggatgctgc
     2521 caataaaatc aatttccgta ctcgaaaaac catgatggtt gcccaacagc tctatgaagg
     2581 aattaatatt ggttctggtg ttcaaggttt gattacctat atgcgtaccg attcgactcg
     2641 tatcagtcct gtagcgcaaa atgaggcggc aagcttcatt acggatcgtt ttggtagcaa
     2701 gtattctaag cacggtagca aggtcaaaaa cgcatcaggt gctcaggatg cccatgaggc
     2761 tattcgtccg tcaagtgtct ttaatacacc agaaagcatc gctaagtatc tggacaagga
     2821 tcagctcaag ctatataccc ttatctggaa tcgttttgtg gctagccaga tgacagcggc
     2881 cgtttttgat accatggctg ttaaattgtc tcaaaatgga gttcaatttg ctgccaatgg
     2941 tagtcaggtt aagtttgatg gttatcttgc catttataat gattctgaca agaataagat
     3001 gttaccggac atggttgttg gagatgtggt caaacaggtc aatagcaaac cagagcaaca
     3061 tttcacccaa ccgcctgccc gttattctga agcaacactg attaaaacct tagaggaaaa
     3121 tggggttgga cgtccatcaa cctatgcgcc aaccattgaa accattcaga aacgttatta
     3181 tgttcgcctg gcagccaaac gttttgaacc gacagagttg ggagaaattg tcaataagct
     3241 catcgttgaa tatttcccag atatcgtaaa cgtgaccttc acagctgaaa tggaaggtaa
     3301 actggatgat gtcgaagttg gaaaagagca gtggcgacgg gtcattgatg ctttttacaa
     3361 accattctct aaagaagttg ccaaggctga agaagaaatg gaaaaaatcc agattaagga
     3421 tgaaccagct ggatttgact gtgaagtgtg tggcagtcca atggtcatta aacttggtcg
     3481 ttttggtaaa ttctacgctt gtagcaattt cccagattgc cgtcataccc aagcaatcgt
     3541 gaaagagatt ggtgttgagt gtccaagctg tcatcaggga caaattattg agcgaaaaac
     3601 caagcgtaat cgcctattct atggttgcaa tcgctatcca gaatgtgaat ttacctcttg
     3661 ggacaagcct gttggtcgtg actgtccaaa atgtggcaac ttcctcatgg agaaaaaagt
     3721 ccgtggtggt ggcaagcagg ttgtttgtag caaaggcgac tacgaggaag aaaagattaa
     3781 ataagaggag gggcccgaaa gtaaatttca ggctcctttt ggttagaact tgacaaaata
     3841 catcatttta tgcgaaacta gaagaagatt attttatact caatgagaat caaagagcaa
     3901 attagaaagc tagtcgcagg ttgctcaaag cactactttg aggttgaaga tggaagctga
     3961 cctggtttga agagattttc gaagagtatt aactaggaga agttatgcgt cttatctatc
     4021 tgataattgg ttttttatca ctggccttgg ctattgttgg ggttgtttta cccttgttgc
     4081 cgacaacgcc tttccttttg ttgtctattg cttgtttctc cagaagttcc aagcgtttcg
     4141 aagactggct ttatcatacc aagctctatc aaacatatgt agctgatttt cgcgagacta
     4201 agtctattac gcgtgaacga aagaaaaaaa tcatcgtctc tatctacgtc ttgatgggaa
     4261 tttctattta ttttgcacct cttttaccag tcaaaatcgg tctgggtgct ttgaccatct
     4321 ttattactta ttatctcttc aaggtcattc cagacaaaga atagttaaaa cagtagttat
     4381 ttgccttgat aaaattgaaa gcatattcat aacaatatga tataataaat ttgaagtaat
     4441 attcaaggag aatcaaatga tttacgaatt ttgtgctgaa aatgtgactt tacttgaaaa
     4501 agcgatgcag gctggagctc gtcggattga actctgtgat aatctagcag ttggtgggac
     4561 aacacccagc tatggagtga ctaaggcagc ggttgaactg gcagctaact acgatacaac
     4621 catcatgacc atgattcggc cacgtggtgg tgactttgtc tataatgacc tagaaattgc
     4681 tatcatgcta gaagacattc gtttgactgc tcaggctgga agtcaagggg ttgtatttgg
     4741 aactttaact gctgataaaa agttggataa gcctaatctg gaaaagttaa ttgctgcatc
     4801 aaaaggaatg gaaattgtct ttcacatggc ctttgatgaa ctaagtgatg aagatcaacc
     4861 ggaagctatt gactggctca gtcaagccgg tgtcactcgt atcctaactc gtgctggtgt
     4921 gtctggcgac tccttagaaa aacgttttgt tcactatcac agaattttgg agtacgctaa
     4981 aggtaaaatt gaaattctac caggtggggg gattgacctt gaaaaccgtc aaacctttat
     5041 cgaccagttg ggggtaacac aattgcatgg tactaaggtt gttttttaaa aaatagaaag
     5101 gaactgctag ctttgggtag cagttttcac ttatgtttga aatttttaaa tcctatcaat
     5161 ttaatcaaga aaaggctcat gcttttggtt ttgtagtaaa tagtggagtc tggacctata
     5221 gttgccagat tttgcaaggt gactttgtca tgactgtgtc catcactgct gataatgtga
     5281 actttcaagt ctttgaccaa gagactgatg acctctatcc tcacgttcat atggaaagca
     5341 tgaggggaag ttttgtcgga aatgtccgtg aggcttgtct ggagattctt taccagattc
     5401 ggaaggcttg ttttgatgtg caagatttta tctgtcatca gactaagcgt attatgactc
     5461 aagttcagga aaagtatgga aaccagttgg agtatctgtg ggaaaaatcg cctgatacag
     5521 ctgtattgcg ccatgaaggc aatcaaaagt ggtatgccgt cttgatgaaa atctcttgga
     5581 ataagctgga aaagggcaga gaaggacaag tggaagcagt caacctcaag catgaccaag
     5641 tagctaattt actttcacaa aaggggattt atccagcctt tcatatgagc aagcgctact
     5701 ggattagtgt gtcccttgat gatactttat cagatgaaga agtactggaa ttgatagaaa
     5761 aaagttggaa cttaacctct aaaaaatgaa atattttaat aattttcatg aactttcaat
     5821 tagctaaata ttctttactg aagaaatttt cagaaaatat agaatttacc acaccagggg
     5881 aatatggtgc catcttcaaa tacctgattg agaatgtcaa gacggatcgt cagatcatct
     5941 gttcgcctca ctgtcatgat gacctcggaa tggcagtggc aaatagcctt gctgctgtca
     6001 agaatggtgc aggacgtgtt gaagggacta tcaatggtat tagggagcga gctgaaaatg
     6061 ctgctttgga agaaattgca gtggctctca atattcgcca agattactac caagtagaaa
     6121 ccagtattgt cctaaatgag accatcaata cgtcagaaat ggtttctcgc ttctctggta
     6181 ttccagttcc taaaaacaaa gccgtcgttg gtggcaatac cttctcccat gaatctggta
     6241 ttcaccaaga tggagtcctt aaaaatcctc tcacttatga gatcatcaca cctgaattgg
     6301 ttggtgttaa gattccgctt ggaaaattat ctggtcgcca tgcttttgtt gagaaactga
     6361 gagaattggc cctagatttt acagaagagg atatcaaacc actctttgct aagttcaagg
     6421 cactggccga taagaagtaa gaaatcacag atgcagatcc tcgagctttg gtagctggaa
     6481 ccatggttga aaatccagaa ggcttccact ttgatgattt acaacttcaa actcatgcag
     6541 ataatgacat tgaagcgctc gttagcctag ccaatatgga tggtgagaaa gtcgaattta
     6601 atgcgacagg gcaaggttcc gttgaagcaa tctttaacgc tatcgataag ttctttaacc
     6661 aatctgttcg tttggtgtcc tacactatca atgcggtaac agatggaatc gatgcccagg
     6721 atcgggtttt ggtcactgtt gaaaacagag atacagaaac catctttaat gcagcagggc
     6781 ttgattttga tgtgttgaag gcttctgcta ttgcctatat aaacgctaat acctttgttc
     6841 aaaaagagaa tgcatgtgag atgggacgca gtgtttctta ccacgatatg cctagtgtgt
     6901 aaaggagaag gctatggcaa agaaaatagt agctctagta ggagacggaa ttggcccaga
     6961 aatcatggag gctggtttag aagttctgga ggctctagct gaaaaaacag gttttgacta
     7021 tgagattgac agacgaccgt tcggaggtgc agatattgat gcagcaggac ctcccttacc
     7081 tgatgaaacc cttaaggcaa gtagggaagc agatgttatc ctactagcag ctatcggtag
     7141 tcctcagtat gatggagcag cggttcgccc tgaacaaggc ctgatggctc tccgtaagga
     7201 actcaatctt tacgctaata ttcgtcctgt aaaaatcttt gacagtctca agtatttgtc
     7261 accacttaaa ccggaacgaa tttctggtgt agactttgtc gtggtgcgtg aattgacagg
     7321 cgagatttac tttggagatc atattcttga agagcgcaaa gcgcgtgata tcaacgacta
     7381 tagctatgag gaagtggagc ggattattcg caaagccttt gaaattgcaa gaaatcgcag
     7441 aaaaatcgtt actagtatcg ataagcaaaa tgttctagcg acctcaaaac tctggcggaa
     7501 agtagctgag gaagtcgcac aggatttccc agatgtaacc ttggaacacc agctggtaga
     7561 ctcagctgct atgcttatga ttagcaatcc tgctaagttt gatgttattg taacggagaa
     7621 tctttttgga gatattttat ctgatgaatc aagcgtctta tctggtacac ttggggttat
     7681 gccatcagcc agtcattctg aaaatggacc aagtctctat gaacctattt acggttcagc
     7741 acctgatatt gcaggtcaag gaattgccaa tcctacttcc atgattttat cagttgccat
     7801 gatgttgaga gatagtttcg gacgttatga ggatgaagag cgtatcaaac gtgctgttga
     7861 gacaagtctg gcggcaggaa ttttaacgag agatataggg ggggcaggct tcaacaaagg
     7921 aaatgacgga agctattatt gcaaggttat gaagttagac gaaaaaatta ctctagtcct
     7981 tttgatttgg aatgtcatca ttttcttgat ttatggcatt gacaaattca aggcaagaag
     8041 aagaacttgg cgcatccaag agaaaatctt actcatttta gcccttactt gtggtggttt
     8101 tggggcctgg ttagctggaa tcacttttca ccacaagact cgaaaatggt actttaaaac
     8161 agtttggttt cttgggatgg tgaccacact agtagcctta tattttattt ggaggtaatg
     8221 gatggcaggg tcttcgaggg aacacgctgc ttgggctcta gcgaactatg gttttaaggt
     8281 cgtgattgca ggaactttcg gtgacattca ttacaataat gaactcaata atggcctgtt
     8341 gccaatcgtt cagcctagag aggttagaga gaaactagcc cagctaaaac caaccgacca
     8401 tgtaactgtg gacttggaac aacaaaaaat catctcacca gttgaagaat tcaccttcga
     8461 gatagatagc gagtggaaac ataaactcct aaatagtttg gatgatatcg gtattacctt
     8521 gcagtatgaa gagttgattg ctgcttatga aaaacaacga ccagcctact ggcaggatta
     8581 gaaaaaatag aaaaggaaat atagtaaact gaaataagat gtaaacaaat gaattagagc
     8641 ttaacatcca tttccagcaa ttttttagaa actacagtgg actattctgg attcaacaca
     8701 ttataaaatt atgacaagac acattcacaa gaaggctacg acattttaaa aggtgagggc
     8761 ggatgtatcg tttgccctac taaagttggt tacattatca tgaccagtga caaggcagga
     8821 cttgagcgta agttcgcagc caaagaacgt aagcgtaaca aaccaggtgt tgttctctgc
     8881 ggtagcattg atgaactttg cgctttagcg caactcaacc cagaaattga agcattctac
     8941 taaaaacatt gggatgaaga tattcttctt ggttgtatcc ttccttggaa accagaagcc
     9001 tttgaaaaac tcaaagcata cggggatggc cgtgaagaac ttatgactga tgtacgtggt
     9061 actagctgtt ttgttatcaa gtttggaaaa gcaggtgaac aattggctgc caagctttgg
     9121 gaagaaggta aaatggtcta cgcctcatct gcttcaatga caaaacgatt gaaactcgct
     9181 atgagcaagg tgtaatggtg tctatggtcg ataaggacgg caaactcatc ccagaacaag
     9241 gaggagcacg ttcaacttca ccagctccag ttgtgatccg taaagggctt gacattgata
     9301 tagtaaaatg aagtaagagc aggacaaatt aatcaggaca gtcaaatcga tttctaacaa
     9361 tgttttagaa gtagaggtgt actattctag tttcaatcca ctataaaatc atgatgcacc
     9421 tgtcagatac ttttaactca tgggactacc gtcaggttga gtattattag gatagagaag
     9481 aagtctagtg ttaaggacat tacagctcat aatactaggc ttttttatag aaattctttt
     9541 ctttttctat aggatatggt attctatgta gaaaatatat gttaataagt aatgccaata
     9601 tttaaacatc attagtaaaa ggagttagat tgatgaataa aagaaaagtt agtttagaag
     9661 atttttataa atggtatagt ctaaataaag aagagttatt aaataaggca actgttggtg
     9721 aaaagtttaa tgataaatta aaagaagagt ttctccagga atggcctttg gataggattt
     9781 taacaatgtc aatcgatgaa tatgtaatag gaaagggaca gcaaaataag tctttatgct
     9841 acgctcttga gaagggaaaa tacaaaaatc tatttcttgg aatttctggt ggctcagctt
     9901 caaaatttgg tatttattgg aataaaaaaa caaacaaata taaagatcaa gctaataatg
     9961 agatttcaga gttggatcag cgattttcaa aattaaaatc agatttgtat gaaattatca
    10021 aagaaggtat tcgttttaac tttgaaaatc ctatttttga tatgaaaaga tcaacaaatg
    10081 aatttattgg tcgttctgct atggtgacaa aattactttg tatctatact gagggagatc
    10141 ctttctttgg tgtaaatatt aatagtcaga aagaattttg gaaccacttt gtttctcaga
    10201 caaatcaagg tggaccttat ctgcaaaatc ataaaataat tgaactgatg tccaaaactt
    10261 atcctgagtt ggagccatcg aaattaggaa ctatgctttt tgagtattct aagcttttta
    10321 tggaaaataa ggaagacaat agtacaatgg attcatcaaa caattttcgt catcaattaa
    10381 ctcaatctct attaaagtct ccaaacctta tccttcgtgg tgctcctggc acaggaaaga
    10441 cttatcttgc taaagaaatt gccaaagaat taacggatgg caacgaagat caaatcggat
    10501 ttgtacaatt tcacccatca tatgattata cggattttgt agaaggttta agaccagtat
    10561 caaatgggga tggagctatt gagtttaggc tacaggacgg tatttttaaa gatttttgtc
    10621 agaaagcaaa agaaacccaa ttgattggag gacaagataa ttttgatgag gcttgggatt
    10681 cttacttaga atatataaat gttgctgaag aaaaagaata tataacaaaa acatcttact
    10741 tatctgttaa tagtagacaa aatttgtcag taaattatga tagtggtgtt ccaggatggt
    10801 cactacctag caaatatgtt tacgagttgt ataaagataa aaattataat aagcaagaat
    10861 actacaaaag tggtggaaaa actgtcctag aaacattgag aaagagattt ggtttgaaag
    10921 actatgtttc cccaacagaa attgatactg ataagaaatt cgttttcatc atcgatgaaa
    10981 tcaatcgtgg ggagatttct aagatttttg gcgaactctt tttctctatc gaccctggct
    11041 atcgtggtga aaaaggaagt gtttctaccc aatatgcaaa tctacacgaa actgatgaaa
    11101 agttctatat ccccgaaaat gtttacatca tcggaactat gaatgatatt gatcgttcag
    11161 tggatacctt tgattttgct atgcgtcgtc gttttcgttt tgttgaagtt actgccgagg
    11221 gtcaagttgg catgttggat aaagagctga atatccatgc agaagaagca aaaattcgtc
    11281 taagaaactt gaacgctgct atcgaaaatg ttcaggaatt aaacagtcat tatcatattg
    11341 gaccaagtta ttttcttaag ttgaaggatg tagattttga ctatgaatta ctctggtccg
    11401 attatattaa gcctctccta gaagactact tgcgaggttc ttatgatgag gttgaaactt
    11461 tggaaacttt gaaaaaagca tttgaactga caaataatga gcaaaaagat caggcagtag
    11521 ctgatgacaa tgaaagcgat gaaaacgatg atgcggatta ctgataatca acacaagatt
    11581 attaaagaaa aatttgttga agaatatcct aaactaagca atcttctttt agacagaacc
    11641 ttggaaagtc tatcccaaga tgaacgtatt ttcatttttc caaatgattt gactcatact
    11701 ccagatttgg ataaaaaaca aaagattttg gaaacgatca atcaggaaat caagacagga
    11761 aatgtgattg gttttcttgg atatggtcag gaaagattaa cgatttcctc acgattttct
    11821 gatgagagta atgaccattt tttgcattat ctcttaaaca aggttcttca tatcaatctc
    11881 actagtttag atgttgcttt gtctcgtgaa gagaggcttt atcaactttt gatgtatctc
    11941 ttccccaagt atctacaagc tgctattcga aaaggtcttt ataaggaata tcatcgattt
    12001 tctcataacg acagtcatgt taagggagtg attgatgtaa gaaaccatct caagaaaaat
    12061 cttcctttca cgggaaatat tgcctacaca acgagagagt tcacctatga taatcccctc
    12121 atgcagttgg tccgtcacac tattgaatac attaagaatc agaaaagcat tggtcaaggg
    12181 gtactagata atctctcaac tagtcgtgaa aacgtatctg aaatcgtgcg tgtaacgccc
    12241 tcttataaac tagctgatcg tgctaagatt attcggggaa atcaatctaa acctatacgt
    12301 catgcatact ttcacgagta cagaaactta caagaacttt gtctgatgat cctaaaccaa
    12361 gaaaagcacg gtttagggta tcaagatcaa aaaatctatg gtattctctt tgatgttgcc
    12421 tggctttggg aagagtatgt ttacaccttg ttgccaaaag gttttgtaca tcccagaaat
    12481 aaggataaga cggatggaat ttcagtattt tctgttggga aacgaaaagt atatccagat
    12541 ttttatgaca gagaacgaaa gattgttcta gatgcaaaat ataaaaaact ggaattgact
    12601 gaaaaaggaa tcaatcgtga ggacttattc cagctgattt cctattctta tattttaaaa
    12661 gctgagaagg ctggacttgt ttttccgagc aaggacaagg ttattgataa tgagatggga
    12721 aacctagcag gttatggagc tttgttgaag aaatggtcta tccaaattcc tcagaagtct
    12781 tcatcttata gtgcattttg taaaatgatg gaaaattcag aagaaaattt taaagcgatt
    12841 attgatgaag aagtggggag aaaataattt cccctctttc ttttattttt aacacaagta
    12901 aaacccgaac aatatatttc aattcataaa ataatttgac aaaaactgta ctttggttta
    12961 taataaatta aggaaacgtc ggaaaagacg tagtgatgcg agagcatagg taggtcatta
    13021 caaaagaaac gagacatcga tatgttaaat gaatttccaa tttttgatta cgaagatatt
    13081 caattgattc caaataaatg tgtcattaaa agccgtgcag aagcggatac aagtgtcact
    13141 ttaggaaatc acacctttaa actacctgtt gtgccagcga atatgcagac gattttggat
    13201 gaaaatgtag cagagcaact ggctaaaggt ggttacttct acattatgca ccgttttgat
    13261 gaggcaggac gcattccttt tattaaacaa atgcacgatc aagggctcat tgcttccatc
    13321 tctgtcggtg ttaaggacta tgagtatgat tttgttagcc agctcaagac tgatgctcct
    13381 gagtatatca ctattgatat tgctcatggt catgcggata gcgtgatttc tatgattcaa
    13441 catatcaaga aagaattgcc agataccttt gtcattgctg gaaatgtggg aacaccagaa
    13501 gctgtgcgtg aattggaaaa tgctggtgcg gatgctacta aggtcggaat cggtcccggt
    13561 aaggtttgta tcaccaaggt taagactggt tttggtacag gtggttggca gttggctgct
    13621 ctacgctggt gtgccaaggc tgcgcgtaaa ccgattatcg ctgatggagg aattcgtact
    13681 cacggtgata ttgctaagtc tatccgcttc ggtgctagca tgatcatgat tggttccctc
    13741 tttgcaggac acattgaaag tccagggaaa acgattgaag tcgatggtga acagttcaaa
    13801 gaatattatg gttcagcctc acaatatcaa aaaggtgctt acaaaaatgt ggaaggcaaa
    13861 cgtatcttgc ttcctgctaa aggtcatctg caagacactt taactgagat ggaacaagac
    13921 cttcaaagtg ctatttctta tgcaggtgga cgtcaggttg ctgaccttaa acacgttgat
    13981 tatgtgatcg tgaaaaactc catctggaat ggggatgctt cccactaaga cggggtcttt
    14041 gaccaaaaaa cttgttatta gagcaaattt ctgttataat aaaacaagtt tccaccctta
    14101 gtgtaatgga tatcacgtaa gattccggtt cttgagatgg gggttcgatt ccctcagggt
    14161 ggatgtaaac atcctaaaaa gcctttaata gggctttttt ctttatcctg acccaaattt
    14221 gccgctaaaa ctgagaatct ctgtctaaag aattcccaaa tgctttccag tcccgtttta
    14281 gagtgactca gggggctttt tttgatataa taaaaaggac tgttatcagt tagaaagagg
    14341 ttggtatgaa agaattacaa actgtactaa agaatcattt tgcaatcgaa tttgcagaca
    14401 aaaatttact ggaaactgcc tttactcata cgagttatgc caatgagcac cgcctcttaa
    14461 aaatttcaca caatgaacgc ttggaatttt taggagacgc tgttctacag ttattgattt
    14521 cagaatatct atataaaaaa tatcctaaaa agcctgaagg tgacctatca aaactccgtg
    14581 ctatgattgt ccgtgaggag agtttagctg gttttgcgcg tgattgccag tttgaccagt
    14641 ttatcaagtt gggtaaaggg gaagaaaaat caggtggtcg caatcgtgac accattcttg
    14701 gtgatgcctt tgaagccttt cttggtgccc ttcttttgga taaggatgtg gccaaggtca
    14761 aggaatttat ctatcaagtc atgattccta aggttgaagc aggcgaattt gagatgatta
    14821 cagactataa aacccatctc caagagttac ttcaggtcaa tggtgatgtg gctattcgtt
    14881 atcaggtgat ttctgaaaca gggcctgctc acgataaggt ctttgatgta gaagttcttg
    14941 ttgaaggtaa gagcatcggt caaggccaag gtcgttctaa gaaattagca gagcaggaag
    15001 ctgccaaaaa tgccgttgag aaggggctgg attcatgtat ttaaaggaaa tcgaaattca
    15061 ggggttcaag tcttttgctg ataagaccaa ggtcgttttt gaccaaggtg tgacggcagt
    15121 tgttggaccc aatggatctg gaaagtccaa tattacagaa agtctgcgtt gggctttggg
    15181 ggagtctagt gtcaagagtc tccgtggggg caagatgccg gatgtcatct ttgctggaac
    15241 agaaagtcgc aaaccgctca attatgcttc tgtagttgtg actctggata atcatgacgg
    15301 atttatcaag gatgcaggtc aagaaatcag ggtggaacgc catatctatc gtagtggaga
    15361 tagcgaatac aagattgacg gcaagaaagt ccgtctgcgt gatattcatg accttttctt
    15421 ggatacaggt ttgggacgag attccttctc tattatttcc caagggaagg ttgaggagat
    15481 ttttaattcc aagtctgagg aacgacgagc tatttttgaa gaagctgctg gagttttaaa
    15541 atacaagact cgcagaaaag aaaccgagag taaactgcag caaactcagg ataatctgga
    15601 ccgcttagag gacattatct acgagttgga taatcaaatc aagcctcttg agaagcaagc
    15661 tgagaatgcc cgtaaatttc tagacttaga aggtcaacgc aaggctattt acttggatgt
    15721 attggttgct caaatcaagg aaaataaggc agaactagag tcgacagaag aagagttggc
    15781 tcaggttcaa gaactcttga tgagttatta ccaaaagcgt gaaaaattag aagaagaaaa
    15841 tcaaactctt aaaaagcaac gccaagattt acaggctgaa atggccaaag accaaggcag
    15901 tttgatggac ttgactagtc tgattagtga tttagaaaga aaattagccc tatcgaaact
    15961 ggagtctgag caagtggcct tgaatcaaca ggaggcacaa gctcgtttgg ctgctttgga
    16021 ggataagaga aattcactca gcaaagaaaa gtctgataaa gaaagctctt tagctctgtt
    16081 agagggaaat ctagtccaaa ataatcaaaa actcaatcgt ttagaagctg aattgctggc
    16141 tttctcagac gatcctgatc agatgattga gctcttacgt gaacgctttg tagctctttt
    16201 acaagaagaa gcggatgtct caaaccagtt gacccgtatt gagaatgagt tggaaaatag
    16261 tcgtcagctt tctcaaaaac aagcagatca actagaaaag ctgaaagagc aattagctat
    16321 agctaaagag aaggctagtc agcaaaagga agagcttgaa actgccaagg agcaggttca
    16381 gaaattattg gctgactacc aagctattgc caaggagcaa gaggagcaga aaacttccta
    16441 tcaagctcaa caaagccaac tctttgaccg tctggataat ctcaaaaaca agcaggccag
    16501 agctcaaagt ttggaaaata tcctgagaaa tcatagtaac ttttatgcag gtgttaagag
    16561 tgttctccaa gaaaaagatc gcctaggtgg gattattggt gcagtcagtg agcatctgac
    16621 ctttgatatg cattatcaaa ctgccctaga aattgcgctt ggagcgagta gccaacatat
    16681 catcgtagaa gatgaagagt cggcaaccaa ggctattgac ttccttaaac ggaacagagc
    16741 cggtcgtgca acctttcttc ctttgaccac tatcaaggcg cgtacgattt ctagtcagaa
    16801 ccaagatgct atcactgtaa gcccaggttt ccttgggatg gcagatgagt tggtgacttt
    16861 tgatactaga ctggaagcca ttttcaagaa cttgctagct acgacggcta tctttgatgc
    16921 cgtagaacat gcgcgtgcag cagctcgcca agttcgttat caggttcgta tggtgacatt
    16981 ggatgggaca gaattacgca cgggtggttc ctatgcgggt ggtgccaatc gccaaaataa
    17041 cagtattttc atcaagccag aactggagca attacaaaaa gaaattgctg cagatgaagc
    17101 aagcttgcgt tcagaagaag cggctttgaa gaccttacaa gaccagatgg ctacattgac
    17161 agaaagatta gaagtcatca aatctcaagg agaacaggca cgtattcagg agcaaggctt
    17221 gtccctcgct tatcagcaaa ctagtcagca agttgaagaa ctggaaactc tttggaaact
    17281 ccaagaagag gaaatagatc gtctttccga gggagattgg caagcggata aggaaaaatg
    17341 ccaagatcgt cttgctgcaa tcgccagtga caagcaaaat ctggaagctg agattgaaga
    17401 gattaagtct aacaaaaatg ccatccaaga acgctatcaa aacttgcagg aagagctagc
    17461 gcaagctcgt ttgcttaaga cagaactgca agggcaaaaa cgttatgaag ttgctgatat
    17521 tgaacgctta ggcaaggaat tggacaatct tgattttgaa caagaggaaa tccagcgcct
    17581 tcttcaagaa aaggttgata atcttgagaa ggttgataca gaattgctca gtcaacaggc
    17641 ggaagaagcc aaaactcaga aaacaaatct ccaacaaggt ttgattcgca agcagtttga
    17701 gttggatgat attgaaggtc aactggatga tattgctagt catttggatc aggctcgcca
    17761 acagaatgaa gagtggattc gcaagcaaac acgtgctgaa gccaagaaag aaaaggtcag
    17821 cgagcgcttg cgctatttac aaagtcaatt aacagaccaa tatcagatta gctatactga
    17881 agctctagaa aaggcgcatg aattggaaaa cctcaatctg gcagagcaag aggtgcagga
    17941 tttagagaaa gctattcgct cactggggcc tgtcaatata gaagctattg accagtacga
    18001 agaagttcac aatcgtctgg atttcctaaa tagccaacga gatgatattt tgtcagcgaa
    18061 aaatctgctc cttgagacca ttacagagat gaatgatgag gtcaaagatc gctttaaatc
    18121 aacctttgag gccattcgtg aatcctttaa agtgaccttc aaacagatgt ttggaggagg
    18181 tcaggcagac ttaatcttga ctgagggaga cttgttgaca gctggggttg agatttctgt
    18241 tcaacctcca ggtaagaaaa tccagtcgct taacctcatg agtggtggtg aaaaagcctt
    18301 atcagctctt gctttgcttt tttctatcat tcgagttaag actatcccat ttgtcatctt
    18361 ggatgaggta gaggccgcgc tggacgaagc caatgttaaa cgttttgggg attacctcaa
    18421 tcgatttgac aaggacagcc agtttatcgt cgtaacccac cgtaagggaa ccatggcagc
    18481 ggctgattct atctatggag tgaccatgca agaatcgggg gtctcaaaaa ttgtttcggt
    18541 taagttaaaa gatttagaaa gtattgaagg atgacaatta aactagtagc aacggatatg
    18601 gacggaacct tcctagatgg gaatggacgc tttgatatgg atcgtctcaa gtctctcttg
    18661 gcttcctaca aggaaaaagg gatttacttt gcggtggctt cgggtcgggg atttctgtct
    18721 ctggaaaaat tatttgctgg tgttcgtgat gacattattt tcatcgcgga aaatggcagt
    18781 ttggtagagt atcaaggtca ggacttgtat gaagcgacta tgtctcgtga cttttatctg
    18841 gcaacttttg aaaagctgaa aacgtcacct tatatagata tcaataaact gctcttgacg
    18901 ggtaagaagg gttcatatgt tctagatacg gttgatgaga cctatttgaa agtgagtcag
    18961 cattataatg aaaatatcca aaaagtagcg agtttggaag atatcacaga tgacattttc
    19021 aaatttacaa ccaacttcac agaagaaacg ctagaagctg gtgaagcttg ggtcaatgat
    19081 aatgtccctg gtgtcaaggc tatgacaact ggctttgaat ctattgatat tgttctggac
    19141 tatgtcgata agggtgtagc tattgttgaa ttagctaaaa aacttggcat cacaatggat
    19201 caggtcatgg cttttggaga caatcttaat gacttacata tgatgcaggt tgtgggacat
    19261 cctgcagctc ctgaaaatgc acgaccagag attttagaat tagcaaagac tgtgattggt
    19321 caccataagg accagtcggt tatagcttat atggagggct tataatggca gatataaaat
    19381 taatcgcatt ggacttggac ggtaccttgc tgactactga taaaaggctg acggatcgca
    19441 ccaaggcaac tttgaaagct gcgcgtgatc gtggtatcaa ggtcgtattg acaactggtc
    19501 gtcccttaaa agccatggac ttctttctcc atgagttagg gactgacgac cacgaagatg
    19561 agtataccat tacttttaat ggtggattag ttcagaaaaa tacaggagaa atccttgata
    19621 aaacagtctt ttcatatgat gatgtggcac gtttgtatga agaaacagag aaattatcac
    19681 tgcctcttga cgctatctca gaaggaactg tttatcaaat tcaatcggac caagaaagtc
    19741 tttatgccaa attcaatcca gctttgacct ttgttccagt ggactttgca gatctgtcta
    19801 gtcaaatgac ctacaacaaa tgcgtgactg cctttgctca agaacccttg gatgcagcca
    19861 ttcagaagat ttctccagaa ttgtttgacc agtatgaaat ctttaaatca cgtgaaatgt
    19921 tgctagagtg gtcaccaaag aatgttcata aagcaacagg cttggcaaaa ctgatcagcc
    19981 atcttggaat tgatcaaagt caagtgatgg cctgtggtga tgaagccaat gacctctcta
    20041 tgattgaatg ggcagggctc ggtgttgcta tgcaaaacgc tgttcctgaa gtcaaggctg
    20101 tcgcaaatgt agtgacgcca atgaccaacg atgaggaagc tgtcgcctgg gctatcgaag
    20161 aatacgtgct aaaggagaac taagatatgg gattgtttga ccgtctattc ggaaaaaaag
    20221 aagaacctaa aatcgaagaa gttgtaaaag aagctctgga aaatcttgat ttgtctgaag
    20281 atgttgatcc taccttcaca gaagttgagg aagtttctca ggaagaagca gaggttgaaa
    20341 ttgttgaaca agctgtgttc caagaagagg aaatccaaga cacagttgaa gaaagtctgg
    20401 atttagagcc agttgtagaa gtttctcaaa aagaagtcga agaatttcca cactcagaag
    20461 aagggaatac tgagtttcta gcgactgtag aagaaaataa ttctgaaatt cttgaagcag
    20521 aaaggcctca aacagaagaa actgttcagg aaaaatatga ccgcagtctt aagaaaactc
    20581 gcacaggttt cggtgcccgc ttgaatgcct tctttgctaa cttccgctct gttgacgaag
    20641 aatttttcga ggaactggaa gaactgttga ttatgagtga tgttggtgtc caagtcgctt
    20701 ctaacttaac ggaggaacta cgttacgaag ccaagcttga aaatgccaag aaacctgatg
    20761 cacttcgtcg tgtcatcatt gagaaattgg ttgagcttta tgaaaaggat ggtagctacg
    20821 atgaaagcat ccacttccaa gataacttga cagttatgct ctttgttggt gtgaatggtg
    20881 ttgggaaaac aacttctatc ggaaaactag cccaccgcta caaacgagct ggtaagaagg
    20941 tcatgctggt tgcagcagat accttccgtg cgggtgcagt agctcagcta gctgaatggg
    21001 gccgacgagt agatgttcca gtagtaactg gacctgaaaa agctgatcca gccagcgtgg
    21061 tctttgatgg tatggaacgt gccgtggctg aaggtatcga tattctcatg attgatactg
    21121 ctggtcgtct gcaaaataag gataacctta tggctgagtt ggaaaagatt ggtcgtatta
    21181 tcaaacgtgt tgtgccagaa gcaccacatg aaaccttctt ggcacttgat gcatcaacag
    21241 gtcaaaatgc cctagtacag gccaaagaat tttcgaaaat cacaccttta acgggaattg
    21301 ttttgactaa gattgatgga actgctcgag gaggtgtggt tctagccatt cgtgaagaac
    21361 tcaatattcc tgtaaaattg attggttttg gtgaaaaaat cgatgatatt ggagagttta
    21421 actcagaaaa ctttatgaaa ggtctcttgg aaggtttaat ctaatcagaa gcaaaaatcc
    21481 tgcaaggcat aaacttgcag gaaatttttt tattctaagc gaccatcttg acgataggtg
    21541 atatctggtt gccaagtcca tttggcaccg aatttttcaa gtaggtcaaa gctggcttga
    21601 ggtcccatgc ttccagcttt atagtcatga agtggggcac cattttcagc ccagagcttt
    21661 tcaatacggt caatcaactt ccatgacgca caaacttcat cccagtggct aaagttagtt
    21721 gagttgttat ttaggacatc ataaatcaat ttttcgtatg gttctggaga agcaccagtt
    21781 gcagtcgcat ctgtacggta atcaagtgag ttaggagcca agttaaattc ttctcctact
    21841 tgcttcccat ttaggctaag agagaagcct tctgttggtt gaatatagat ggtcaaaata
    21901 tttggagcaa gtggttctcc aaagatagaa tccatttgtt taaagacgat gttgacatga
    21961 gttccttttt cagtcagtcg tttacctgta cggaaaaaga aaggaacacc acggaatcga
    22021 tcgctgtcta caaagaaggc accagatgta aaggtttcag ttgttgattc tggattcaca
    22081 tttggctcgc tacgataaga gatgtatttc atgccatcaa tcttaccaga gcggtattgc
    22141 ccacggataa agtgttcttt gagttcttca tcagttggat gatagaggtt tttaaagacc
    22201 ttaatctttt cagcacgaat ctcgtctttt gtgaagcttg ctggtttgtc catggcgagg
    22261 agcgaaagaa gttgtagagt gtggttttgg accatgtcac ggagggcacc ggattggtca
    22321 tagtagccac cacgttcttc tacacccaag cgctccgcaa aggtaatttg aacattgtcg
    22381 ataaaatcct tgttccaaac gttttcaaaa atcaagtttg caaagcgaac tgcaaagatg
    22441 ctttggatca tttccttacc aagataatgg tcgatacgga aaatttgttc ttcgtcaaat
    22501 gttgctagga gttcgtcatt caacttgttt gcagttgcgt aatctgtacc aaatggtttt
    22561 tcaacgatca agcgctcaaa acctttgcca tcgacaatgt tttcagattt gaggtgtttg
    22621 gcaatggttc caaagaactg aggtgccata gacaagaaga agagcttatt gtgttcagct
    22681 tggtattttt cattaagctc agcttgtaat tgacgcaaag caatataatg ttccgaatca
    22741 ttgacatcat ggctttgata gtagaagtgg ctagcaaatt cttgggcttg ctcggtacta
    22801 tctgccaaat caaggatgga ctcgacaact acagattcaa aatattcctt actccaaggt
    22861 ctacgggcag ttccaataac ggcaaagtgc ttggaaagat tgccggattg atatagtcta
    22921 aaaagggaag ggtagagctt gcgtttagcc aggtctccac tcgcaccgaa aattgtaaca
    22981 ataaccttag atgacatcta gctacctaat ttctattttt attcctagtc ttgctaggta
    23041 tgaggaaacc tcatttcata aacttaattc taacataatt ttccgaaaaa tcaaaaattc
    23101 caatacgagg tagaaaaact gcaaggaaat ccttacagtt tgagtttaga cgtttaagac
    23161 cttatctaag aactctttca gacgagggtg ttgtgggtta tcaaagattt ggtcaggtgt
    23221 tccgtcttca aggaactcgc catctgcagt aaagataacg cggttggcaa cctgacgagc
    23281 aaatcccatc tcatgggtta cgataatcat ggtcatacct tgctcagcca attccttcat
    23341 aacgttaagt acgtctccaa ccatctcagg gtcaagggca gaagttggtt catcgaagag
    23401 catgatgtct ggattcattg ctaggccacg agcgatggcc acacgttgtt tttgaccacc
    23461 tgataggcta tctggattgg cattagcttt atctgctagt ccaacctttt caagcaactc
    23521 cattcccaat tcctcagctt cttccttagt catcaacttg tgctcaatag gagcaaaggt
    23581 gatgttgtcc aatacagaca tatgagggaa gaggttgaag tgttggaata ccatgccgat
    23641 attttcacgg acgtggtcaa cattggttgt tttttcagtt aaatcatagc cgttcacagt
    23701 gatgtgaccg ctagtgactt cttctaaaag attgaggcta cggaggaaag ttgacttacc
    23761 agaacctgaa ggaccgatga tacaaacaac atctccttca tagaacttag tcgtaattcc
    23821 ttttaggact tcattttttc catagtgctt gtgtaaatca tttacatcaa tttttaattt
    23881 tgccattaac gaatcctctt ttctaagcgt ttcgctagtc tagtcaaaag tgtgataatt
    23941 acaagataga agatagcaag gattgcatac atcttgaaac tttggtagtt acgagcaatg
    24001 ataatcttac cagtttggaa gagttcaacc aaaccgatag cagatacgat agttgtatct
    24061 ttaagagcga taacgaattg gttgacaaag tttggcaaca tcaatttagt tgcttgtggc
    24121 aagataatct tacgcatggt ttttccataa gagataccca agcttcggct ggcttccatt
    24181 tggccaactg gaacggcctg aataccacca cgaacgattt cagcgatata agcagccgca
    24241 ttgagtgaga gggcaattgt tccagctaca aagtcgttaa ttgggctttg ttggcctgtg
    24301 atagactcga tgaagtttgg aattccccag aagatgaagg ctgcaagaat catcaatgga
    24361 ataccacgaa taacgtcaac gaaaatctca gagatgacgc gaagagattt gtatgggcta
    24421 acgctaaaca taccgaagat aattccgatg acaatggcaa tagcaaatga gataagagct
    24481 agagcaagag tgataccaag accgctaagg agttgtttgt agttgttttg aagcaagccc
    24541 cagagcgttg tttcgtcaac agtacttgtt gaagcagttg aagattcgct agctaggtat
    24601 ttgtcaagaa tcttttggaa ttcaccgttt gctttaaggt ttgcaagtcc gttgttgaac
    24661 atttcaatca gttctggatt tgctcctttt ttaacggcaa aggctgtttc accgattgga
    24721 gttccagaga ttggagtttt caatttttga ccttggctga tagaatattt gagaacaggt
    24781 tcatcatcca taacggcatc aatggcacca gtgtttaaac tgtcatacat tgaagaacca
    24841 tcagcaaagg ttttgatttt gtaaccgtat ttgctttgat tttctgttag gaaggtttga
    24901 gaagcagttc cgtttttaac accgactgtc tttcctttta gatcttcata agaagcaata
    24961 ttgcttgatt ctttgacacc aagaatggta ttagcagtgt agtatgattc tgagaagtca
    25021 aaagttgcct tacgagcatc tgtgacagac ataccagcaa tgataccatc ggcttgacca
    25081 gcttggacag cactgatagc agcatcaaaa ccagggttgg tgatttcaat ttcaaaacct
    25141 tggtctttag cgattgcctt aatcaattcc atatcaatac cagtgtattg gttgcttgaa
    25201 ttttggaaaa caaaaggtgc aaaagaagaa tcgctggcaa tgatatattt agccttaaca
    25261 ggaatagctt ttaatcctgc gagagtagtt gtagttggca ctgctgaaga tgatgaagca
    25321 gtccatttct tgataatttt atcaagacta ccatcttttt tcatttcagc caaggcttgg
    25381 ttaaattcag taaccaggtg ctcgtattta cttccttttt tcacaccgaa agcaaaactt
    25441 cctacagctt caccatccat ttcaatatgg aggtcttgac cttggttaat ggcatattcg
    25501 ataacaggtt tgtcatccat catggcatcg atggcaccag cactcaagct gttgttcatt
    25561 aaatccccag tgtcaaatgt tttaatagta aagccgtatt tatctttgat tgtttcaagg
    25621 aaacgttgag cggcagttcc gtttttaaca ccaacggttt tgccagttaa ttggtcgtac
    25681 ttgctaattt tgtgtgactt tgtagtagca atgacaactt ttgtatcata gtaagtatca
    25741 gacatggtga agactttttc acgttcttta gtctttgtca tccctgccat gatagcgtcg
    25801 gcttgcccag cttgaaccgc attgactgct gcgtcaaatc caggatagga catctgaatg
    25861 ttccagcctt taatctcagc gactttgtta ataatgtcaa catcaattcc tttataagtt
    25921 tgatctgaat ctttaaactc aaaaggtgca taggcggtat cagaaacaat cttaatcgtt
    25981 tctgctttgg caatacctaa tgagaaaatt gggaataaaa ttagcaaaaa tgctagaaat
    26041 tttttcttca tttttagtct ccttttccga atattctctc attatatcag aaaaagtaaa
    26101 aaaaggcaaa tttttatatt tttcgtgtca gaaaatataa cattggtttt tccttttctt
    26161 tcttgaattg ctattaaaaa gtgctataat aatagtatat atagaagaaa agaggacggg
    26221 gatatgaaga acaaaagaat atttaaagac ttccaagctt caaaaatgag tttaaacatt
    26281 tacacaagcc ccttgttagc ctttgttttt gtcttcatag gagagtttgt ggcttatact
    26341 ttatatggta ttagtttgtt agctctcatc ggacttgcta gaaattttgg agaggctggt
    26401 caaaatcttg caagctactt gcagaccttg catcagagct tgacggataa aacaagtgac
    26461 tttcgtttaa ttttaggatt actggccttt ggttttattc ttaacactgt gttcagatgg
    26521 acaagaaaag ttgagaaaag acctattcga accttgggat tttatagaga gaatttcctc
    26581 agcaatcttc tgaaaggatt tagtctaggc ctggcacttt ttcttctgac cttgttaggt
    26641 ttggtggtct taggtcaata tcgtttggaa tccattcact tgaatcctta ttctcttgcc
    26701 tttgtcgtct ttactatccc attttggatt ttacagggga cagcagaaga agtggtggcc
    26761 cgtgcttggc tacttcctca attggcctca agaaccaatc taaaactagc tattcttata
    26821 tctagcctgt tctttaccct gcttcatatg ggcaattctg gcctcacccc tctatctcta
    26881 gtaaatctct ttttattcgg agttgccatg gctctttacc ttctcaaaac tgatacagtt
    26941 tggggtgttg caggtattca tggtgcttgg aattttgctc agggtaatct ctttgggatt
    27001 ttagttagtg gtcaaccgtc aggaacgtct ctgatgacct ttttaccaca aggcaatcaa
    27061 gattggctat caggtggttc ttttggcata gaaggttcca ttatgacaag tctggtatta
    27121 ctactgctga ttgtctatct tgctaataaa ttaaagaaag aaaatgaaag gatgtgactt
    27181 cggtccgtcc ttttcttcgt gaaaatacta taagtatgct aaaataggaa tagcacatgg
    27241 agagaggatt cttatgatca atcacattac agataatcaa tttaaactag tatcaaaata
    27301 tcaaccatca ggagatcaac cccaagctat cgagcagttg gtggataaca ttgagggggg
    27361 agaaaaagct cagattctga tgggggcgac tggaacaggg aagacctata ctatgagtca
    27421 ggtcatttct aaagtcaata aaccaactct ggttattgcc cacaataaaa ctctggctgg
    27481 tcagctctat ggggagttta aggaattttt ccctgaaaat gcagttgagt atttcgtatc
    27541 ctactatgat tattaccagc cagaggccta tgtcccttct agcgatacct atattgagaa
    27601 ggatagttct gtcaatgacg agattgacaa gcttcgccac tcagctacct cagcactttt
    27661 ggagcgtaat gatgttattg tcgtggcctc agtctcttgt atctatggtt tgggttcgcc
    27721 caaggaatac gctgatagtg tcgttagtct ccgtcctggt ctagagattt ctcgtgataa
    27781 actcttgaat gacttggtcg atattcagtt tgaacgcaat gatattgatt tccaacgcgg
    27841 aagatttcgc gttcgtgggg atgtggtaga gattttccca gcttcccgag atgaacatgc
    27901 ctttcgagta gaattttttg gagacgaaat tgaccgtatt cgtgaagttg aggctctgac
    27961 aggtcaggtg ttgggagaag tggatcattt agcgattttc ccagcgacac actttgtgac
    28021 caatgacgac catatggaag ttgccattgc aaagattcag gccgagttgg aagaacaatt
    28081 agctgtcttt gaaaaggaag gtaaactgct tgaagcccag cgtttgaaac agcggacaga
    28141 gtatgatatc gaaatgttgc gtgagatggg ctataccaat ggggttgaga actattcacg
    28201 acacatggat ggacggagcg aaggagagcc tccttatacg ctcttagatt tcttcccaga
    28261 tgatttctta attatgattg acgagagtca catgaccatg gggcaaatca agggcatgta
    28321 caatggagac cgttcgcgta aagaaatgct ggttaattat ggcttccgat tgccgtctgc
    28381 tttggacaat cgtcctctcc gtcgggagga gtttgagagt cacgttcatc agattgttta
    28441 cgtttcagcg acacctggtg actatgaaaa tgaacagact gagacagtga ttgagcaaat
    28501 cattcgtcca acgggactct tggatcctga agtggaagtc cgtccgacta tgggacagat
    28561 tgatgacctc ttgggtgaaa tcaatgcccg cattgaaaaa aatgagcgta cctttatcac
    28621 aactttgacc aagaaaatgg cagaggactt gaccgactac ttcaaggaaa tgggtatcaa
    28681 ggtcaagtac atgcactcgg atatcaagac cttggaacgg acggagatta tccgtgacct
    28741 gcgcttgggt gtctttgatg tcttggtcgg aattaacctg ctccgtgaag gaattgacgt
    28801 tcctgaagtg agcctcgtag ctattctcga tgctgataag gaaggtttcc ttcgcaacga
    28861 acgtggactc atccagacta ttggacgtgc tgctcgtaac agtgaaggcc atgtcatcat
    28921 gtatgcagac acggttaccc agtctatgca acgtgctatc gatgaaactg cccgccgtcg
    28981 gaaaattcag atgacctata atgaagagca tgatatcgta ccacagacaa tcaagaaaga
    29041 aatccgtgac ctgattgccg tgaccaaggc agttgctaag gaagaggaca aggaagtcga
    29101 catcaatagc ctcaacaaac aagagcgcaa ggaactcgtc aagaaactag aaaaacaaat
    29161 gcaagaagcc gtcgaagtgc ttgactttga actggcggct cagattcgtg atatgatgtt
    29221 ggaagtcaag gtcttggatt aggggaatag tatgatttat ttaagaaagt taaagagaga
    29281 agatttgatg tctttatggg aaatggctta ttcacaactt aatccagttt ggaaacagta
    29341 tgatgctccc tattatgatg attatcagta tttttcaaat tttaaagaat tcgaactaca
    29401 aaaatcagaa tccattttaa gcaactcaaa tcgccttggt atttttgttg atgataaact
    29461 agttgggact gtttcgcgtt attgggtatg taaacaaaca agatggatgg aattgggaat
    29521 tggtatttat gataaaaaat tctggaacac tggtattggg aaagttgcta tgttgcagtg
    29581 gatagatagg acgtttcagg attacttgga gttggagcat ctgggtttga caacttggtc
    29641 agggaatctt ggtatgatga aacttgctga aaaattaaga atgaaaaaag aagctcatat
    29701 tccaaaagtt cgttattatc aaggtaaata ttttgatagt attaaatatg gtattttgag
    29761 agaagactgg gagaaaataa atgacggtta ttatcaaatc aatggaaact cctgaagaga
    29821 tagaaggtaa atcccttgtt cactggcaaa cgtggagaga ggcttatgat gaccttttgc
    29881 ctgcagaatt tcaggagaca atgacattag aaagatgtcg attctttagt caaaagtatc
    29941 cagaaaatac attgattgcg ataggtgatc tgaaggtagt tggttttatc agttatggta
    30001 actttcgtga tgagactatt caagctggtg aaattattgc tttatatgtt ttaaaagact
    30061 attacggaaa aggcatagca caaaagttaa tgaaagcagc tttgactgct ctgaatcatt
    30121 tttctgaaat tttcttatgg gtattgaaag ataacaagcg agccattgct ttctatcaaa
    30181 aaatgggttt tacttttgat ggacaagaaa aaatacttga acttggaaag cctataaagg
    30241 aaaaacgaat ggtgttctat tctaaataat tttaaaaaag taaaagataa tatctctggt
    30301 agtaagtctg aaaatttaat aaattagaaa gcgagtaaat ttatgtcccg ttcccaatta
    30361 acaattttaa caaatatctg tctgattgaa gacctcgaaa ctcagcgcgt ggtgatgcag
    30421 tatcgcgccc ctgaaaacaa tcgctggtct ggttatgcct ttcctggagg tcatgtagaa
    30481 aatgatgagg cttttgcgga gtctgtcatt cgtgaaatct acgaagaaac agggttgact
    30541 atccaaaatc ctcaacttgt cggcattaaa aattggccac tagatacagg tgggcgctat
    30601 attgtcattt gttataaggc gactgagttc tctggtaccc ttcaatcttc agaagaggga
    30661 gaagtttctt gggtgcaaaa agaccagatt ccaaacttaa atctggccta tgatatgcta
    30721 ccattgatgg aaatgatgga agctcccgac aagtcagagt ttttctatcc tcgccgtaca
    30781 gaagacgatt gggaaaagaa aatcttctag tcttttacta aataacctag ctgatccaag
    30841 gcctcctcga tatagtggag gtcttgttgt gtttcggctt cgactaggtg gtaatggatg
    30901 ccatctgtca attcagatag aggtctaaag tcagaatgct cgacttgttc tagaaaatgt
    30961 tgcacatctc tgcgacaaga cagtttaagc aaggtttcaa tttctccata aacgggatgg
    31021 tcaatcaagg tattctggac atgcccacca ttatcgacaa tagcaaggag ttcctgaccg
    31081 atttcttcaa cttcatgttt cactttgaaa agtttgtgaa cataaggatt ggtatcaatt
    31141 tgcttataga tgtagccacg attggtggat aggataggag cgccatcggc tctcaaaatt
    31201 gcaatgtcct gcacaatgac ctgacgtgtg acatgaaaat gttccgccaa actttggcca
    31261 ttaagggctt taggagcttc tttcaagagt tggagcaggg cttgtttgcg atcttttgtc
    31321 atagcttttc cttttaacgg cgttttcgaa gcactttata gacagctagt gctaatgtat
    31381 agtctaccat actatggata attgtgccaa atccaactag tacaaataga acatagaaca
    31441 tattttctac attggtaccc gaagttgcgt aaaaaaggac acaggctaat acttcagcaa
    31501 gggcatgaac aacagccaaa acaaaattga aaacccagga agattttggt ttatctaggg
    31561 tatcggggaa tttttgtagg taaagagctc ctaaagcacc aaaagatata tgggaaaaag
    31621 cccgaaaaac gataaccatg ggatagccag ccatcaaaaa tccaaaacta gaggctagga
    31681 tgacaaaaac tgccatcaag ggcgacaaga acatggctat aaaaatagcg atgtggctcc
    31741 ccaaagtata ggaagcaggt ggaatgacaa tcttgaaagg cataacaatt ggaatcaaaa
    31801 tcgcaatagc cgttaaaagg gctgtcattg tcataaattg tgtctttttc cgtgtattca
    31861 caagaatctc ctttttaact gcatatacac tagtatggta caataaacca gacaataaag
    31921 caagaattta cttgggttta tagatcattt tttagttaaa agttatagta gatggaaa
//
LOCUS       DAVBCG010000010        31049 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBCG010000010 DAVBCG010000000
VERSION     DAVBCG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.010"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..504
                     /locus_tag="VQ495_000389"
     CDS             <1..504
                     /locus_tag="VQ495_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2530395.1"
                     /translation="VTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIEVIALDCT
                     KRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTLSGYTSYS
                     PKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAITRPKEITE
                     RFVASLK"
     gene            523..2040
                     /locus_tag="VQ495_000390"
     CDS             523..2040
                     /locus_tag="VQ495_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2530396.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            2165..3493
                     /locus_tag="VQ495_000391"
     CDS             2165..3493
                     /locus_tag="VQ495_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2530397.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            3745..4611
                     /locus_tag="VQ495_000392"
     CDS             3745..4611
                     /locus_tag="VQ495_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2530398.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            4627..5466
                     /locus_tag="VQ495_000393"
     CDS             4627..5466
                     /locus_tag="VQ495_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2530399.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            5738..6190
                     /locus_tag="VQ495_000394"
     CDS             5738..6190
                     /locus_tag="VQ495_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2530400.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            6287..6631
                     /locus_tag="VQ495_000395"
     CDS             6287..6631
                     /locus_tag="VQ495_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530401.1"
                     /translation="MVSSKHAARASFLDGQGITVDETSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            6915..7559
                     /locus_tag="VQ495_000396"
     CDS             6915..7559
                     /locus_tag="VQ495_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2530402.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVISCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            7728..8645
                     /locus_tag="VQ495_000397"
     CDS             7728..8645
                     /locus_tag="VQ495_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2530403.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLITDKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            8663..9547
                     /locus_tag="VQ495_000398"
     CDS             8663..9547
                     /locus_tag="VQ495_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2530404.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(9563..10414)
                     /locus_tag="VQ495_000399"
     CDS             complement(9563..10414)
                     /locus_tag="VQ495_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2530405.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            10614..12671
                     /gene="pbp2b"
                     /locus_tag="VQ495_000400"
     CDS             10614..12671
                     /gene="pbp2b"
                     /locus_tag="VQ495_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2530406.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            12682..13278
                     /gene="recR"
                     /locus_tag="VQ495_000401"
     CDS             12682..13278
                     /gene="recR"
                     /locus_tag="VQ495_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2530407.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVATMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            13454..14497
                     /locus_tag="VQ495_000402"
     CDS             13454..14497
                     /locus_tag="VQ495_000402"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2530408.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            14581..15954
                     /locus_tag="VQ495_000403"
     CDS             14581..15954
                     /locus_tag="VQ495_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2530409.1"
                     /translation="MKLTIHEVAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGVRDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            15941..16552
                     /locus_tag="VQ495_000404"
     CDS             15941..16552
                     /locus_tag="VQ495_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2530410.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDPLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            16643..17347
                     /locus_tag="VQ495_000405"
     CDS             16643..17347
                     /locus_tag="VQ495_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2530411.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            17566..18939
                     /gene="ftsA"
                     /locus_tag="VQ495_000406"
     CDS             17566..18939
                     /gene="ftsA"
                     /locus_tag="VQ495_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2530412.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENIIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            18956..20215
                     /gene="ftsZ"
                     /locus_tag="VQ495_000407"
     CDS             18956..20215
                     /gene="ftsZ"
                     /locus_tag="VQ495_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2530413.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            20220..20891
                     /locus_tag="VQ495_000408"
     CDS             20220..20891
                     /locus_tag="VQ495_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2530414.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            20901..21440
                     /locus_tag="VQ495_000409"
     CDS             20901..21440
                     /locus_tag="VQ495_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2530415.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            21440..21703
                     /locus_tag="VQ495_000410"
     CDS             21440..21703
                     /locus_tag="VQ495_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2530416.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            21700..22485
                     /locus_tag="VQ495_000411"
     CDS             21700..22485
                     /locus_tag="VQ495_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET2530417.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            22494..23282
                     /gene="divIVA"
                     /locus_tag="VQ495_000412"
     CDS             22494..23282
                     /gene="divIVA"
                     /locus_tag="VQ495_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2530418.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            23630..26422
                     /locus_tag="VQ495_000413"
     CDS             23630..26422
                     /locus_tag="VQ495_000413"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2530419.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            26832..27194
                     /locus_tag="VQ495_000414"
     CDS             26832..27194
                     /locus_tag="VQ495_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530420.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            27729..28421
                     /locus_tag="VQ495_000415"
     CDS             27729..28421
                     /locus_tag="VQ495_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2530421.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            28474..30885
                     /locus_tag="VQ495_000416"
     CDS             28474..30885
                     /locus_tag="VQ495_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2530422.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
BASE COUNT         9350 a         5640 c         6879 g         9180 t
ORIGIN      
        1 gtcactaaac ttccaatcat tgggattatc aaacgtgatt atccacctca ggaacccttc
       61 atcacggcta ctatgaaaga agttgatgaa ttggcagaac tggacatcga ggtgattgct
      121 ctggattgta ccaagcgtga acgctacgat ggtttggaaa ttcaagagtt cattcgtcag
      181 gttaaggaga aatatcctaa tcagcttttg atggctgata ctagtatctt cgaagaaggg
      241 ctagcagctg tagaagcagg aattgacttt gtcggaacaa ccttatcagg ctacacatcc
      301 tacagtccaa aagtagacgg tccagatttt gaattgatta agaaactctg tgatgccggt
      361 gtagatgtca ttgcagaagg aaaaattcat acaccagaac aagccaaaca aatccttgaa
      421 tatggagtgc gaggcatcgt tgttggtggc gccattacta gaccaaaaga gattacagaa
      481 cgcttcgttg ctagtcttaa ataagatgtg agggggagtt ttatgtttaa agttttacaa
      541 aaagttggaa aagcttttat gttacctata gctatacttc ctgcagcagg tctacttttg
      601 gggattggtg gtgcactttc aaacccaacc acgatagcaa cttatccaat actagacaat
      661 agtatttttc aatcaatatt ccaagtaatg agctctgcag gagaggttgt attcagtaat
      721 ttgtcactac ttctctgtgt gggattatgt attggcttag cgaaacgaga taaaggaacc
      781 gctgcgttag caggagtaac tggttactta gttatgactg caacgatcaa agctttggta
      841 aaacttttta tggcagaagg atctgcaatt gatactggag ttattggagc attagttgtc
      901 ggaatagttg ccgtatattt gcacaaccga tataacaata ttcaattacc ttccgcttta
      961 ggattctttg gaggttcacg cttcgttcct attgttacat cgttctcttc tatcttgatt
     1021 ggctttgtct tctttgttat ttggccacct ttccaacaac ttcttgtttc tacaggtgga
     1081 tatatttctc aggcgggtcc aattggaact tttctatatg gatttttaat gagactttct
     1141 ggagcagtag gcttacatca tataatttac cctatgtttt ggtatactga acttggtggt
     1201 gttgaaactg ttgcaggaca aacagtggtt ggagctcaaa aaatattttt tgctcaatta
     1261 gccgatttgg cccattctgg attatttaca gaaggaacaa ggttttttgc aggtcgtttc
     1321 tcaacaatga tgttcggttt accggctgcc tgtttagcga tgtaccatag tgttcctaaa
     1381 aatcgtcgta aaaaatacgc gggtttgttt tttggagttg ctttaacatc ttttattacc
     1441 ggtattacag aaccaattga atttatgttt ctattcgtca gtccggttct atatgttgtt
     1501 cacgcattcc ttgatggtgt tagcttcttt attgcagacg tcttaaatat ttcaatagga
     1561 aacacatttt caggaggtgt aatcgatttc actttatttg gaattttgca ggggaacgct
     1621 aagacgaatt gggttcttca gattccattt ggacttattt ggagtgtttt gtattatatt
     1681 atttttagat ggttcattac tcaattcaac gttctaacgc cagggcgagg agaagaagta
     1741 gattctaaag aaatttctga atccgcagat tcaacttcaa atactgcaga ttatttaaaa
     1801 caggatagcc tacaaattat cagagccttg ggtggatcaa ataatataga agatgtagat
     1861 gcttgtgtga cacgtttacg tgtagctgta aaagaagtta atcaagttga taaagcactt
     1921 ttaaaacaaa ttggtgcagt tgatgtctta gaagtgaagg gtggcattca agcaatctat
     1981 ggagcaaaag caatcttata taaaaatagt attaatgaaa ttttaggtgt agatgattaa
     2041 gtacttactg acttaataaa aaacagagga gagtgatgga tgagtaggat gaaatgaaat
     2101 cgcatacaag aaataaagaa ctcattatcc aagttggata cgcttattac ataggagaat
     2161 acaaatgaaa tttagaaaat tagcttgtac agtacttgcg ggtgctgcgg ttcttggtct
     2221 tgctgcttgt ggcaattctg gcggaagtaa agatgctgcc aaatcaggtg gtgacggtgc
     2281 caaaacagaa atcacttggt gggcattccc agtatttacc caagaaaaaa ctggtgacgg
     2341 tgttggaact tatgaaaaat caatcatcga agcgtttgaa aaagcaaacc cagatataaa
     2401 agtgaaattg gaaaccatcg acttcaagtc aggtcctgaa aaaatcacaa cagccatcga
     2461 agcaggaaca gctccagacg tactctttga tgcaccagga cgtatcatcc aatacggtaa
     2521 aaacggtaaa ttggctgagt tgaatgacct cttcacagat gaatttgtta aagatgtcaa
     2581 caatgaaaac atcgtacaag caagtaaagc tggagacaag gcttatatgt atccgattag
     2641 ttctgcccca ttctacatgg caatgaacaa gaaaatgtta gaagatgctg gagtagcaaa
     2701 ccttgtaaaa gaaggttgga caactgatga ttttgaaaaa gtattgaaag cacttaaaga
     2761 caagggttac acaccaggtt cattgttcag ttctggtcaa gggggagacc aaggaacacg
     2821 tgcctttatc tctaaccttt atagcggttc tgtaacagat gaaaaagtta gcaaatatac
     2881 aactgatgat cctaaattcg tcaaaggtct tgaaaaagca actagctgga ttaaagacaa
     2941 tttgatcaat aatggttcac aatttgacgg tggggcagat atccaaaact ttgccaacgg
     3001 tcaaacatct tacacaatcc tttgggcacc agctcaaaat ggtatccaag ctaaactttt
     3061 agaagcaagt aaggtagaag tggtagaagt accattccca tcagacgaag gtaagccagc
     3121 tcttgagtac cttgtaaacg ggtttgcagt attcaacaat aaagacgaca agaaagtcgc
     3181 tgcatctaag aaattcatcc agtttatcgc agatgacaag gagtggggac ctaaagacgt
     3241 agttcgtaca ggtgctttcc cagtccgtac ttcatttgga aaactttatg aagacaaacg
     3301 catggaaaca atcagcggct ggactcaata ctactcacca tactacaaca ctattgatgg
     3361 atttgctgaa atgagaacac tttggttccc aatgttgcaa tctgtatcaa atggtgacga
     3421 aaaaccagca gatgctttga aagccttcac tgaaaaagcg aacgaaacaa tcaaaaaagc
     3481 tatgaaacaa tagtccttag ttattctata aaaagtagtt ttttaaagaa cctaagagtg
     3541 tataccccct tttccctcta cacagatagt gtaagaaaag ggggcttttg tttaaaatgt
     3601 aagaaactgt cacgaaatta aaatgaagtt cttacataag cgaatcataa aaaatttcat
     3661 tttgatttta aaacagttca agaaagtcaa aaaattattc tatttgaaag agaggtgccg
     3721 actgtgaaag tcaataaaat ccgtatgcgg gaaacagtga tttcctacgc tttcctagca
     3781 ccagtattat tcttctttgt catctttgtg ttggctccga tggtgatggg cttcattaca
     3841 agtttcttta actactcaat gactaaattt gagtttgtag gcttggataa ctatatccgt
     3901 atgtttaaag atcctgtctt tacaaaatct ctgattaaca cagttatttt ggttattgga
     3961 tctgtaccag ttgttgttct attctcactc tttgtagcat ctcagaccta tcatcaaaat
     4021 gtcattgcca gatccttcta ccgtttcgtc ttcttccttc ctgttgtaac gggtagtgtt
     4081 gccgtgacag ttgtttggaa atggatttat gacccactat cagggattct aaactttgtc
     4141 cttaagtcca gccacatcat cagccaaaac atttcttggt tgggagataa aaactgggca
     4201 ttgatggcga ttatgattat tctcttgacc acttcagttg gtcagcccat catcctttat
     4261 atcgctgcca tggggaatat tgacaattca ctggttgaag cggcgcgtgt tgatggtgca
     4321 actgagtttc aagttttttg gaagattaaa tggccaagcc ttcttccaac aactctttat
     4381 attgcaatca tcacaacaat taactcattc cagtgtttcg ccttgattca gcttttgaca
     4441 tctggtggtc caaactactc aacaagtacc ttgatgtact acctttacga aaaagccttc
     4501 caattgacag aatacggcta tgccaacaca attggtgtct tcttggcagt catgattgct
     4561 atcgtaagct ttgttcaatt taaagtactt ggaaacgacg tagaatacta aagaaaggag
     4621 acagctatgc aatctacaga aaaaaaacca ttaacagcct ttactgttat ttcaacaatc
     4681 attttgctct tgttgactgt gctgttcatc tttccattct actggatttt gacaggggca
     4741 ttcaaatcac aacctgatac aattgttatt cctcctcagt ggttccctaa aatgccaacc
     4801 atggaaaact tccaacaact catggtgcag aaccctgcct tgcaatggat gtggaactca
     4861 gtatttatct cattggtaac catgttctta gtttgtgcaa cctcatctct agcaggttat
     4921 gtattggcta aaaaacgttt ctatggtcaa cgcattctat ttgctatctt tatcgctgct
     4981 atggcgcttc caaaacaagt tgtccttgta ccattggtac gtatcgtcaa cttcatggga
     5041 atccatgata ctctctgggc agttatcttg cctttgattg gatggccatt cggtgtcttc
     5101 ctcatgaaac agttcagtga aaatatccct acagagttgc ttgaatcagc taaaatcgac
     5161 ggttgtggtg agattcgtac cttctggagt gtagccttcc cgattgtgaa accagggttt
     5221 gcagcccttg caatctttac cttcatcaat acttggaatg actacttcat gcaattggta
     5281 atgttgactt cacgtaacaa tttgaccatc tcacttgggg ttgcgaccat gcaggctgaa
     5341 atggcaacca actatggttt gattatggca ggagctgccc ttgctgctgt tccaatcgtc
     5401 acagtcttcc tagtcttcca aaaatccttc acacagggta ttactatggg agcggtcaaa
     5461 ggataatact ctgcgaaaat ctcttcaaac tacgtcagcg tcaccttgcc atacttaagt
     5521 attgcctgcg gttagcttcc tagtttgttc ttcaattttc attgagtata ggaaaatcaa
     5581 tctatcaaga tacagaagta tattttatag atttagagaa tatagaggtt ataagtgtct
     5641 acaaaatgga gggtatgcag ttactttatg aagttttgtc agacacttat aaacttaaga
     5701 atggttttag ttaactatca gaaacgaagg aaagagtatg atttttgacg atttgaaaaa
     5761 catcaccttt tacaaaggga ttcatcctaa tttagacaag gctatcgact atctctacca
     5821 acatcgtaag gattctttcg aattaggaaa gtatgagatt gatggagata aagtctttct
     5881 agttgttcag gaaaatgtcc tcaatcaagc tgaaaatgat caatttgagt atcataagaa
     5941 ctatgcagat ttgcatttgc tggtagaagg acatgaatat tcgagctacg gttcacgtat
     6001 caaagacgag gcagtagcat tcgacgaagc gagtgacatt ggctttgttc attgtcatga
     6061 acactaccca ctcttgttgg gttatcacaa ttttgcgatt ttcttcccag gtgagccaca
     6121 tcagccaaat ggttatgcag gcatggaaga aaaggttcga aaatatctct ttaaaatttt
     6181 gattgattaa aaataggatg aattgttttt ttgtaaagct ttgacaatac tctaccatga
     6241 aattgatctt tgtgaggtag agaaatgaga ataaaatatt taaaaattgg tatcttctaa
     6301 gcatgctgca agagctagtt tcttagatgg acagggaatt acagttgatg agacgtcctg
     6361 gataattaat ggcattgtga atgcattgat tggtagatac ataaaattag gtacttatgc
     6421 agctaagtat ggtattagta tagcacgctc gatcttaagt agggcagctg caactgcagc
     6481 agcaagagta ggattactga ccaagatttc tggatggatt ttacgagtag ctgtgaatgt
     6541 agctgatgta tatggtaatt ttgccaacaa tattgctgca gcttgggatg catatgataa
     6601 aattcctaac aatggtcgta taaactttta aaatgcgaga atgaaagcac tttgtatttt
     6661 tttattgaat atgttagctt ggacagtgct tgcaatgata attcgtggag ggctagatgg
     6721 atttgatgag catacttgga gtactatttt aattgcttcg ctgttcgggg tatatgatta
     6781 taagcccata gataaaaata gaaaaaagtc caaaagaaaa aagaaaaaat agatttgttc
     6841 atggtaggga cttatgaaag ctttactgac aaaaaagaaa acagtttaca aagaaaaatg
     6901 atggaggagc aaacatggca caaaaaggag taagccttat caaggcagca tttgatacag
     6961 ataactttct catgcgtttt agtgagaaag tcttggacat cgtgacagcc aatcttcttt
     7021 ttgtcatctc ttgtttgccc atcgtgacga ttggagtggc taaaatcagc ctctacgaga
     7081 ccatgttcga agttaagaag agcagacggg tgcctgtttt taaaatctat ctaagatctt
     7141 tcaagcaaaa tctgaaacta ggtcttcagc tgggtttaat ggagttagga attgtgtttc
     7201 ttaccctttc agacctctat cttttctggg gtcaaacagc tctgcccttc caattgctga
     7261 aagccatttg tttaggtatt ctgatttttc ttactatcgt gatgctggct agttacccta
     7321 tcgcggcacg ttatgaccta tcttggaaag aaattcttca aaaaggattg atgttggcta
     7381 gttttaactt tccttggttc ttcctcatgt tagccattct tgtcctcatt gtgatggttc
     7441 tttatctgtc cgccttcagt ctactcttag gtggctcagt cttcctactt tttgggtttg
     7501 gactattggt ctttatccag actggattga tggagaaaat tttcgcaaaa taccaatagg
     7561 agccttattt ctgaaactac tttcaaaggt tccaaacgct attctataag cgagaaacta
     7621 aaatctaagt gtctataaga tattgaaagc cttttcggta aagtgtatac taaagaagta
     7681 aagaaaaatc tgctttagca gaaaataaaa ataataggag aaaatctatg tcagatttga
     7741 aaaaatacga aggtgtcatt ccagccttct acgcatgtta tgatgatcaa ggagaagtaa
     7801 gcccagaacg tacgcgtgcc ttggttcaat acttcattga taaaggtgtt caaggtcttt
     7861 atgtcaatgg ttcttctggt gaatgtatct accaaagcgt tgaagatcgc aagttgattt
     7921 tggaagaagt tatggcggtt gccaaaggta aattgaccat tattgcccat gttgcctgca
     7981 acaataccaa ggatagtatg gaacttgctc gccatgctga aagcttggga gtagatgcta
     8041 ttgcaacgat tccaccaatt tatttccgct tgccagaata ctcagttgcc aaatactgga
     8101 acgatatcag ttctgcagct ccaaatacag actacgtgat ttataacatt cctcaattgg
     8161 caggtgttgc tttgactcca agcctttaca ctgaaatgtt gaaaaatcct cgtgttatcg
     8221 gtgtgaagaa ctcttctatg ccagttcaag atattcaaac ctttgtgagc cttggtgggg
     8281 aagaccatat cgtctttaac ggtccagatg aacagttcct aggtggtcgc ctcatgggtg
     8341 ccaaagctgg tatcggtggt acttatggtg ctatgccaga actcttcttg aaactcaatc
     8401 agttgattac ggataaggac ctagaaacag cgcgtgaatt gcagtatgct atcaacgcaa
     8461 tcattggtaa actcacttct gctcatggaa atatgtacgg tgtcatcaaa gaagtcttga
     8521 aaatcaatga aggcttgaat attggatctg ttcgttcacc attgacacca gtgactgaag
     8581 aagatcgtcc agttgtagaa gcggctgctg ccttgattcg tgaaaccaag gagcgcttcc
     8641 tctaatctaa aaggaggtat ttatgacata ttacgttgca attgatatcg gtggaaccaa
     8701 catcaagtat ggtttggttg atcaagaggg gcaacttctt gaatcgcatg aaatgccaac
     8761 tgaggcgcat aagggtggac ctcatatctt acaaaagacc aaagatatcg tagctagtta
     8821 tttagaaaaa ggcccagtag caggtgttgc catatcttct gctgggatgg tggatccgga
     8881 taagggtgag attttctatg ctgggccgca aatccctaac tacgcaggca cccagttcaa
     8941 aaaggaaatc gaagaaagct ttactattcc ttgtgagatt gaaaatgatg tcaactgtgc
     9001 aggtcttgct gaggcagtat ctggttcagg caagggagca agtgtgacac tttgcttgac
     9061 cattggaaca ggtatcggtg gttgcttgat tatggatagg aaagtcttcc atggttttag
     9121 caattcagcc tgtgaagtcg ggtatatgca tatgcaggat ggagcttttc aagacttggc
     9181 ttctacaaca gctttagtga aatatgtagc tgaagcccat ggagaagatg ttgatcagtg
     9241 gaatggccgt agaattttca aagaagccac tgaaggaaac aaaatctgca tggaaggtat
     9301 tgaccgtatg gttgactatc taggaaaagg tctggcaaat atttgctacg ttgccaatcc
     9361 agaagtggtc attcttggtg gtggtatcat ggggcaagag gctatcctca aacctaagat
     9421 ccgcacagcc ttgaaagagg ctttggtacc aagtttagca gaaaaaacac gattagaatt
     9481 tgcccatcac caaaatacag cagggatgtt gggtgcatat tatcatttta agacaaaaca
     9541 atcctagttt ggctcagcca aactaggatt ttcttacacg tttttgtcta cgatagccgt
     9601 tgagtttttt attttcccag tagctattaa agattttttc cttgctttcg cgattgattt
     9661 ccaaaaagta ggcataaatc aaatcgataa agaagagcat aggaagttga gcggatattc
     9721 gttggatgta ggagggttgg ctgtgggtgg ctacaagaac agtctctgta taggtctggc
     9781 tatttttatt gggaacactt gtaaagagta cagtctttgc ccccatctcc ttggcatcta
     9841 atagactatc taaaatagaa ggagttgagc ctgaaagtga gaagcccagt actagacaat
     9901 tttcatccat gatgctggtt gtccaggcaa agccgtcttg gtctgtcaaa gcttcgcaga
     9961 ccacacctaa tcgcataaaa cgtaatttca tttcacgggc gacgaggcca gaactccctg
    10021 ttccaaagaa gtagatacgc tcagcatctt cgattagctg ggcaattcgc tctagttgga
    10081 tttcgtcaat caagtcttgt gtttgttccc tcatattgct ataacttctg aggactcgtt
    10141 tggtcagtgg actgtgcttg gagacttggt tggcttgatt ttctgcctga tgttggtatt
    10201 ggaaaataaa ttctcggtag ccagtaaagc cacacttttt agcaaagcgg gtcaaagcag
    10261 cttgagaaat atgtaatttt tgggtgactt gttgagaaga taaatcatct gtaatcgttt
    10321 cagcttgcaa aaaatagcga gcgatttctt gctctaggtc tgtcatttct tcaaaatgtg
    10381 aatcaatgat agttgcgata tctggtttgt ccatagggaa agctccttta catgagtcat
    10441 actggaagac tagatcaggg aatagtcaca cttcattata acacataata taaggataga
    10501 taaataaaaa cgcatctctg ttttaaaaac gaaaaaatcg aaaaagcttc tctcttttcc
    10561 ataattttct actcaaattg tggtacaatt aagagtaaga ttttaagtta gaaatgagac
    10621 tgatttgtat gagaaaattt aacagccatt cgattccgat tcggcttaat ttattgtttt
    10681 caatcgtcat tttactcttt atgaccatta ttggtcgttt gttgtatatg caggttttga
    10741 acaaggattt ttacgaaaaa aagctagcct cagctagtca gaccaagatt acaagcagtt
    10801 cagcccgtgg ggaaatttat gatgctagtg gaaaaccttt ggtagaaaat acgttaaagc
    10861 aggttgtttc ctttacgcgt agcaataaaa tgacggctac agacttaaaa gaaacagcta
    10921 aaaagttact gacttatgtg agcatcagtt ctccaaattt gacagaacgc cagctggcgg
    10981 attactattt ggctgatcct gaaatctata aaaaaatagt ggaagctctc ccaagtgaga
    11041 aacgcttgga ttcagatggc aatcgtctat ccgaatcaga actgtataac aatgcggtcg
    11101 atagtgtaca aacgagtcaa ctaaactata cagaggatga aaagaaagaa atctatcttt
    11161 ttagtcagtt aaatgctgtt ggaaactttg cgacaggaac cattgcgaca gatcctctaa
    11221 atgattctca ggtggctgtt attgcctcta tttcaaagga gatgcctggc attagtattt
    11281 ctacttcttg ggatagaaag gttttggaaa cttccctttc ttctatagtt gggagtgtat
    11341 ccagtgaaaa agctggtctc ccagcggaag aagcagaagc ctatcttaaa aaaggctatt
    11401 ctctaaatga tcgtgtagga acctcctatt tggaaaagca atatgaagag accttacaag
    11461 gaaaacgctc ggtaaaagaa atccatctgg ataaatatgg caatatggaa agcgtggata
    11521 caattgagga aggtagtaag ggaaacaata tcaaactgac cattgatttg gccttccaag
    11581 atagcgtgga tgctttactg aaaagttatt tcaattccga gctagaaaat ggtggagcca
    11641 agtattctga aggtgtctat gcagtcgccc ttaacccaaa aacaggtgcg gttttgtcta
    11701 tgtcagggat taaacatgac ttgaaaacgg gagagttgac gcctgattcc ttgggaacgg
    11761 taaccaatgt ctttgttcca ggttcggttg tcaaggcggc gaccatcagc tcaggttggg
    11821 aaaatggagt cttgtcagga aaccagacct tgacagacca gtccattgtc ttccaaggtt
    11881 cagctcccat caattcttgg tatactcagg cttacggttc attccctatc acagcggtcc
    11941 aagctctgga gtattcatct aatacctata tggtccaaac agccttaggt cttatggggc
    12001 aaacctatca acccaatatg tttgtcggca ccagcaatct agagtctgct atggagaaac
    12061 tgcgttcaac ctttggcgaa tatggcttgg gtactgcgac aggaattgac ctaccagatg
    12121 aatctactgg atttgttccc aaagagtata gctttgctaa ttacattact aatgcctttg
    12181 ggcagtttga taactatacg ccgatgcagt tggctcagta tgtagcaact attgcaaata
    12241 atggtgttcg tgtggctcct cgtattgttg aaggcattta tggtaataat gataagggag
    12301 gactgggtga cttgattcag caactgcaac cgacagagat gaataaggtc aatatatccg
    12361 actccgatat gagcatcttg caccaaggtt tttatcaggt tgcccatggt actagtggat
    12421 tgacaactgg acgtgccttt tcaaatggtg ccttggtatc cattagcgga aaaacaggta
    12481 cagccgaaag ctatgtggca gatggtcagc aagcaaccaa taccaatgcg gtggcctatg
    12541 ccccatctga taatccccaa atcgctgtcg cagtggtctt tcctcataat accaatctaa
    12601 caaatggtgt aggaccttcc attgcgcgtg acattatcaa tctgtatcaa aaataccatc
    12661 caatgaacta gaaaggaaat tatgctttat ccaacaccta ttgccaagtt gattgacagt
    12721 tattctaagt taccaggtat cgggattaag acggctacgc gtctggcctt ttatacgatt
    12781 gggatgtctg ctgatgatgt caatgaattt gcaaaaaatc tcctttctgc taagagagaa
    12841 ttgacatatt gttctatttg tggacgtttg acagacgacg atccttgttc tatctgtacc
    12901 gatccgactc gtgaccagac aacaatttta gttcttgagg atagtagaga tgtggcaacc
    12961 atggaaaata tccaagaata ccatggcctc tatcatgtcc tgcatggcct catttctcct
    13021 atgaatggta tcagtccgga cgatatcaat ctcaagagcc ttatgactcg tcttatggat
    13081 agtgaagttt cagaagtgat tgtggcgact aatgctacag cggatggtga agcgacttcc
    13141 atgtatcttt cacgtttgct caagccggct ggtatcaagg ttacgcgtct agcacgaggt
    13201 ctcgctgtgg gagcggacat tgagtatgcg gacgaagtga cactcttacg agccattgaa
    13261 aatcggacag agttgtaagt gtaggcaaat ttacgaactc cattcattta taaaaaatca
    13321 aagaggctga aaatcgttcc tatcggcctc tttttgtata gtgtgatgag taggatcggg
    13381 ttcaagtttt aaaaaaccaa gcaaatatga tatactaaag agcgagtatt ctagtagaat
    13441 taggacaaat aatatgaaac aaacgattat tcttttatat ggtggacgga gtgcggaacg
    13501 cgaagtctct gtcctttcag ctgagagtgt catgcgtgcg gtcaattacg accgtttcac
    13561 agtcaagact ttctttatca gtcagtcagg tgactttatc aaaacacagg aatttagtca
    13621 tgctccgggg caagaagacc gtctcatgac caatgaaacc attgattggg ataagaaagt
    13681 tgcaccaagt gctatctacg aagaaggtgc agtggtcttt ccagtccttc acgggccaat
    13741 gggagaagat ggctctgttc aaggattctt ggaagttttg aaaatgcctt acgttggttg
    13801 caacattttg tcatcaagtc ttgccatgga taaaatcacg actaagcgtg ttctggaatc
    13861 tgctggtatt gcccaagttc cttatgtggc tatcgttgaa ggcgatgatg tgactgctaa
    13921 aatcgctgaa gtggaagaaa aattggctta tccagtcttc actaagccgt caaacatggg
    13981 gtctagtgtc ggtatttcta agtctgaaaa ccaagaagaa ctccgtcaag ccttaaaact
    14041 tgccttccga tatgacagcc gtgtcttggt tgagcaagga gtgaatgccc gtgaaattga
    14101 ggttggcctc ttgggtaact acgatgtcaa gagcacgcta cctggagaag ttgtcaagga
    14161 cgttgccttt tatgactacg atgccaagta tattgataac aagattacta tggatattcc
    14221 tgccaaaatc agtgatgatg tggtagctgt catgcgtcaa aatgcagaaa cagccttccg
    14281 tgccattggt ggccttggtc tatctcgttg cgatttcttc tatacagata agggagagat
    14341 ttttctcaac gagctcaata ccatgccagg tttcacccag tggtctatgt acccactact
    14401 ttgggacaat atggggatca gctacccaga actaatcgag cgtttggttg accttgccaa
    14461 ggaaagtttt gacaagcgcg aagcgcattt gatataaaat gaaagagagg gtagaagcca
    14521 gaaccatcac tgcaaggtga ctagagttct cggacttcag ccctttttaa aggagtagaa
    14581 atgaaattaa caatccatga agttgcccaa gttgttggag ccaaaaatga tatcagtatc
    14641 tttgaggaca cccagttaga aaaagctgag tttgatagtc gtttgattgg aactggagat
    14701 ttatttgtgc ccctaaaagg tgtgcgtgac ggtcatgact ttatcgaaag agcttttgaa
    14761 aatggtgcgg cagcaaccct gtctgagaaa gaggttgcaa atcatcccta cattctagta
    14821 gatgatgttt tgactgcctt tcaacaacta gctgcctact atcttgaaaa aacagctgtt
    14881 gatgtctttg cggttacggg ttcaaatggc aagacaacga ctaaggatat gttggcgcat
    14941 ttactgtcaa caagatacaa gacctacaaa acacaaggca attacaataa tgagattggc
    15001 cttccttaca cagttctcca catgcctgaa ggaacagaaa agttggtctt ggagatgggg
    15061 caggaccact tgggagatat tcatctcttg tctgaattgg ctcgtccaaa aacagccatc
    15121 gtgaccttgg ttggagaagc tcatttggcc tttttcaaag atcgttctga gattgctaaa
    15181 ggaaaaatgc aaattgcaga cggaatggct tcaggttcct tgcttttagc gccggctgac
    15241 cctatcgtag aggactattt gccaactgat aaaaaggtgg ttcgttttgg tcaaggagca
    15301 gagctggaaa ttactgactt ggttgagcgc aaagatagtc tgaccttcaa ggccaatttc
    15361 ttagagcaag tccttgattt gccagtaact ggcaagtaca atgcgacaaa tgctatgatt
    15421 gcatcctatg ttgccttgca agaaggagtt tcagaggagc aaattcgttt ggccttccaa
    15481 catcttgaat tgacgcgtaa ccgtaccgag tggaagaaag cagccaatgg agcagacatc
    15541 ttgtcagatg tttacaatgc caatccaact gctatgaagc tgattttaga gaccttctct
    15601 gccattccag ccaatgaagg tggcaagaaa attgcagtgt tggcggatat gaaggaactt
    15661 ggtgtccagt ctgttcaact tcataatcag atgattttga gcctttcgcc agatgtgctt
    15721 gatactgtta ttttctacgg agaagatatt gctgaattag cccaacttgc tagtcaaatg
    15781 ttcccaatcg gacacgttta ttacttcaag aaaacagaag accaagacca atttgaagac
    15841 ctagttaagc aggtcaaaga aagccttggg gctcatgacc aaatcctgct caaaggctct
    15901 aactctatga atctagccaa gttggtagaa agtttagaaa atgaagacaa gtgattttgt
    15961 caagtatttg caaagaatga ttgccattac agatactgga ttaaccttta caaaagatcc
    16021 gtttgaccgt gagcgctacg aagacttgcg agaccttcta tctgaaatgt tgaatcaagc
    16081 atcagacctt gattccgaag aagtggcaga agtcttgaag ccaacttctg cttatgcgac
    16141 tcccttaatg gacgtccgtg cttggattgt tgaggacgaa aagatttgtc tggttagggg
    16201 acaaggagag gatagttggg ctttgccagg tggctttggt gaagtcggct attctccaac
    16261 tgaaaatatt ctcaaggaaa ttgaagaaga aaccggtttt aaagccaaag ttgaaagact
    16321 gctagctgtt tttgatacca atcgtttcca actacagagc aaacaatatg caaagtttgt
    16381 ttttgaatgt aagcttctcg atggacaatt ccaagaaaat caagaaatcg ctgaccttca
    16441 attttttgca attgacccac tgccgaattt atctgaaaaa cgcattacca aggagcaaat
    16501 cgagattctt tggcaggttt atcaaggtca gagggagcaa tatcttgact aagaagatga
    16561 ttatcgtatt tctaaattca tttttcttaa gtagcatggt ataataaaat gcaggaaaat
    16621 tttgaatcat gaggaagact agatgaattt atgggatatt ttctttacga ctcaggcaac
    16681 cgagccgccc aaatttgacc ttttttggta tgttagccta tttacgctct tagccttaac
    16741 cttttataca gcccatcgct atcgtgaaaa gaaggtttac caacgatttt tccaaatctt
    16801 gcagactgtt cagttaatcc ttctttatgg ttggtactgg gtcaatcata tgccactgtc
    16861 agaaagccta cccttttacc attgccgtat ggctatgttt gtggtactct tgcttcctgg
    16921 tcagtccaaa tataaacaat actttgcatt attgggaaca tttgggacat tagcagcctt
    16981 tgtttatcca gtgccagatg cttacccttt tccacatatc accattctat cctttatctt
    17041 tggtcattta gcactcttgg ggaactctct agtttatcta ttgagacagt ataatgcgcg
    17101 attgctggat gtgaagggaa tttttctcat gacctttgcc ctaaatgcct tgatttttgt
    17161 ggtcaatttg gtgacaggtg gcgattacgg atttttgaca aaaccgccat tggttgggga
    17221 tcacggtcta gtagctaatt atttacttgt ttcaattgtg ctggtagcta ctatcagttt
    17281 gactaagaaa atcttagaat tctttttagc tcaagaagca gaaaaaatga ttgcaaagga
    17341 agcttaacac agagctttct tttttgctct tagagagttt ttacaagcag cttataaaat
    17401 aagaatttct gaatagacaa actcaaaaaa tggctgggaa atttaggaaa aaagcaagca
    17461 cgattaaatt ttttgtgtta taatattttg tgaatagcta tgcctatgtt tagctatgga
    17521 ataatacgaa gtgcgaaact tggaagatag agaggaagcg atgtaatggc tagagaaggc
    17581 ttttttacag gtctagatat tggaacaagc tctgtcaagg tgcttgtggc cgagcagaga
    17641 aatggtgaat taaatgtaat tggcgtgagt aatgccaaaa gtaaaggtgt aaaggatgga
    17701 attattgttg atattgatgc agcagcaact gctatcaagt cagccatttc ccaagcggaa
    17761 gaaaaggcag gcatttcgat taaatcagta aatgtcggct tgcctggtaa tcttttgcag
    17821 gtagaaccaa ctcaggggat gattccagta acatctgata ctaaggaaat tacggatcaa
    17881 gatgttgaaa atgttgtcaa atcagctttg acaaagagta tgacacctga ccgtgaagtc
    17941 attaccttta ttcctgaaga atttattgtg gatggtttcc aagggattcg tgacccacgt
    18001 ggcatgatgg gggttcgcct tgaaatgcgt ggtttgcttt atacaggacc tcgtactatc
    18061 ttgcacaatt tgcgtaagac ggttgagcgt gcaggtgttc aggttgaaaa tattatcatt
    18121 tcaccactag caatggttca gtctgttttg aatgaagggg aacgtgaatt tggtgctaca
    18181 gtgattgata tgggggcagg tcaaacgact gtcgctacaa tccgtaatca agaactccag
    18241 ttcacacata ttctccaaga aggtggagat tatgtaacta aagatatctc caaggttttg
    18301 aaaacctctc gcaaattagc ggaaggcttg aaactgaatt acggggaagc ctatccgcct
    18361 cttgcaagca aagaaacctt ccaagtagag gttattggag aagtggaagc agtcgaagtg
    18421 acggaagcct acttgtcaga aattatttct gcacgaatca agcacatcct tgaacaaatc
    18481 aagcaagaat tagatagaag gcgtctattg gacctccctg gtggtattgt cttaatcggt
    18541 gggaatgcca ttttaccagg tatggttgag cttgctcagg aagtctttgg cgtccgtgtc
    18601 aagctttatg ttccaaatca agttggtatc cgtaatccag cctttgcgca tgtgattagt
    18661 ttatcagaat ttgcgggtca attaacagaa gttaatcttt tggctcaggg agcgataaaa
    18721 ggtgagaatg acttaagtca tcagccaatt agttttggtg ggatgctgca aaaaacagct
    18781 cagtttgtac aatcaacgcc tgttcaacca gctcctgctc cagaagtaga gtcggtggcg
    18841 cctacagaac caatggcgga tttccaacaa gcttcacaaa ataaaccgaa attagcagat
    18901 cgtttccgtg gcttgatcgg aagcatgttt gacgaataaa gaggaaaaat aaattatgac
    18961 attttcattt gatacagctg ctgctcaagg ggcagtgatt aaagtaattg gtgtcggtgg
    19021 aggtggtggc aatgccatca accgtatggt cgacgaaggt gttacaggcg tagaatttat
    19081 cgcagcaaac acagatgtac aagcattgag tagtacaaaa gctgagactg ttattcagtt
    19141 gggacctaaa ttgactcgtg gtttgggtgc aggaggtcaa cctgaggttg gtcgtaaagc
    19201 cgctgaagaa agcgaagaaa cactgacgga agctattagt ggtgccgata tggtcttcat
    19261 cactgctggt atgggaggag gctctggaac tggagctgct cctgttattg ctcgtatcgc
    19321 caaagattta ggtgcgctta cagttggtgt tgtaacacgt ccctttggtt ttgaaggaag
    19381 taagcgtgga caatttgctg tagaaggaat caatcaactt cgtgagcatg tagacactct
    19441 attgattatc tcaaacaaca atttgcttga aattgttgat aagaaaacac cgcttttgga
    19501 ggctcttagc gaagcggata acgttcttcg tcaaggtgtt caagggatta ccgatttgat
    19561 taccaatcca ggattgatta accttgactt tgccgatgtg aaaacggtaa tggcaaacaa
    19621 agggaatgct cttatgggta ttggtatcgg tagtggagaa gaacgtgtgg tagaagcggc
    19681 acgtaaggca atctattcac cacttcttga aacaactatt gacggtgctg aggatgttat
    19741 cgtcaacgtt actggtggtc ttgacttaac cttgattgag gcagaagagg cttcacaaat
    19801 tgtgaaccag gcagcaggtc aaggagtgaa catctggctc ggtacttcaa ttgatgaaag
    19861 tatgcgtgat gaaattcgtg taacagttgt tgcaacgggt gttcgtcaag accgcgtaga
    19921 aaaggttgtg gctccacaag ctagatctgc tactaactac cgtgagacag tgaaaccagc
    19981 tcattcacat ggctttgatc gtcattttga tatggcagaa acagttgaat tgccaaaaca
    20041 aaatccacgt cgtttggaac caactcaggc atctgctttt ggtgattggg atcttcgccg
    20101 tgaatcgatt gttcgtacaa cagattcagt cgtttctcca gtcgagcgct ttgaagcccc
    20161 aatttcacaa gatgaagatg aattggatac acctccattt ttcaaaaatc gttaagtaaa
    20221 tgaatgtaaa agaaaataca gaacttgttt ttcgagaagt tgcagaggct agtctgagtg
    20281 ctcatcgaga gagtggttcg gtctctgtca ttgcagttac caagtatgta gatgtaccga
    20341 cagcggaagc cttgcttccg ctaggtgttc atcatatcgg tgaaaatcgt gtagataagt
    20401 ttctggaaaa atatgaagct ttaaaagatc gagatgtgac ttggcatttg attggtacct
    20461 tgcaaagacg taaggtgaaa gatgtcattc aatacgttga ttatttccat gcattggact
    20521 cagtaaagct agcaggggaa attcaaaaaa gaagtgaccg agtcatcaag tgtttccttc
    20581 aagtaaatat ttctaaagaa gaaagcaaac acggtttttc gagagaggaa ctgctggaaa
    20641 tcttgccaga gttagccaga ctagataaga ttgaatatgt tggtttaatg acgatggcac
    20701 cttttgaggc tagcagtgag cagttgaaag agattttcaa ggcgacccaa gatttacaaa
    20761 gagaaattca agagaaacaa attccaaata tgcctatgac cgagttaagt atgggaatga
    20821 gtcgtgatta taaagaagcg attcaattcg gttccacttt tgttcgtata ggtacatcat
    20881 tttttaagta ggagagaacc atgtctttaa aagatagatt cgatagattt atagattatt
    20941 ttacggagga tgaggattca agtctccctt atgaaaaaag agatgagcct gtgtttactc
    21001 cagtaaattc ttcacaggaa ccggctctcc caatgaatca accttcacag tcggctggta
    21061 caaaagagaa caatatcacc agacttcatg caagacaaca ggaattggca aatcagagtc
    21121 agcgtgcaac ggataaggtc attatagatg ttcgttatcc tagaaaatat gaggatgcaa
    21181 cagaaattgt tgatttattg gcaggaaacg aaagtatctt gattgatttt cagtatatga
    21241 cagaggtgca ggctcgtcgt tgtttggact atttggatgg agcttgtcat gttttagctg
    21301 gaaatttgaa aaaggtagct tctaccatgt atttgttgac accagtgaat gttattgtaa
    21361 atgttgaaga tatccgttta ccagatgaag atcaacaggg tgagttcggt tttgatatga
    21421 agcgaaatag agtacgataa tgattttttt aattcgtatg atttataatg cagtggatat
    21481 ttactccctg attttggtag ccttcgctgt catgtcttgg tttccaggtg cctacgaatc
    21541 cagtttaggt cgttggattg tagcgttggt gaaaccagtg cttgctccct tgcaacgcct
    21601 gcctttacag atagcgggtc ttgatttatc tgtttgggtc gcgattgttt tggttcgatt
    21661 tttaggagaa aacctagtgc gttttctggc gatgatagga tgaataaagg gatttatcag
    21721 catttctcca tagaagatcg tccatttctt gacaagggaa tggaatggat aaagaaggta
    21781 gaagatagct atgctccttt tttaactcct tttatcaatc ctcatcagga gaagctatta
    21841 aagattttgg ccaaaaccta tggtcttgct tgtagcagta gtggggaatt cgtctcgagt
    21901 gagtatgttc gagttttatt atacccagat tatttccaac cagagttttc agattttgaa
    21961 atatctctcc aggaaattgt gtattccaat aaatttgaac atttaacgca tgctaagatt
    22021 ttagggacag tcatcaatca attagggatt gaacggaaac tttttggaga tatcctagta
    22081 gatgaagaac gggcgcagat tatgattaat cagcagtttc ttcttctctt tcaagatgga
    22141 ctaaagaaaa ttggtcgtat acctgtttcg ctggaggaac gtcctttcac cgagaaaata
    22201 gataagctag aacagtatcg agaactggat ttatctgtgt ctagttttcg attagatgtt
    22261 cttttatcaa atgttttgaa actatctagg aatcaagcaa accagttgat tgaaaagaaa
    22321 cttgtccaag taaattatca tgtggtagac aaatcagatt acactgttca agttggagac
    22381 ttgattagtg tgagaaaatt tggtcgcttg agattacttc aagataaggg acaaacgaaa
    22441 aaagagaaga aaaaaataac cgtccagtta ttattaagta agtgaggaat agaatgccaa
    22501 ttacatcatt agaaataaag gacaagactt ttggaactcg attcagaggt tttgatccag
    22561 aagaagtcga tgaattttta gatattgtgg ttcgtgatta cgaagatctt gtgcgtgcga
    22621 atcatgataa aaatttgcgt attaagagtt tagaagagcg tttgtcttac tttgatgaaa
    22681 taaaagattc attgagccag tctgtattga ttgctcagga tacagctgag agagtgaaac
    22741 aggcggcgca tgaacgttca aacaatatca ttcatcaagc agagcaagat gcgcaacgct
    22801 tgttggaaga agctaaatat aaggcaaacg agattcttcg tcaagcaact gataatgcta
    22861 agaaagtcgc tgttgaaaca gaagaattga agaacaagag ccgtgtcttc caccaacgtc
    22921 tcaaatctac aattgagagt cagttggcta ttgttgaatc ttcagattgg gaagatattc
    22981 tccgtccaac agctacttat cttcaaacca gtgatgaagc ctttaaagaa gtggttagcg
    23041 aagtacttgg agaaccgatt ccagctccaa ttgaagaaga accaattgat atgacacgtc
    23101 agttctctca agcagaaatg gaagaattac aagctcgtat tgaggtagcc gataaagaat
    23161 tgtctgaatt tgaagctcag attaaacagg aagtggaaac tccaactcct gtagtgagtc
    23221 ctcaagttga agaagagcct ctgctcatcc agttggccca atgtatgaag aaccagaagt
    23281 agctccagtg catccgacag gtccaacacc agctacagaa actgttgatt cagcaccggg
    23341 atttgaagca ccgcaagaat ctgttacaat tttataagaa atattctgag aacaatatct
    23401 tatccttata ttcccagcga gcaggagatg gtgtgagtcc tgcattccct atcgataaga
    23461 ttatcctctc aaactatcaa gtctgaatct agtaagattt gacgttcccc acgttacggg
    23521 ataagaggga gaaagactaa aactttttcc gaataaaggt ggtaccacga ttttcgtcct
    23581 ttttggaagt cgtggttttt aatttgttat tatttataaa ggagatacca tgaaactcaa
    23641 agacaccctt aatcttggga aaactgaatt cccaatgcgt gcaggccttc ctaccaaaga
    23701 gccagtttgg caaaaggaat gggaagatgc aaaactttat caacgtcgtc aagaattgaa
    23761 ccaaggaaaa cctcatttca ccttgcatga tggccctcca tacgctaacg gaaatatcca
    23821 cgttggacat gctatgaaca agatttcaaa agatatcatt gttcgttcta agtctatgtc
    23881 aggattttac gcaccattta ttcctggttg ggatactcat ggtctgccaa tcgagcaagt
    23941 cttgtcaaaa caaggtgtca aacgtaaaga aatggacttg gttgagtact tgaaactttg
    24001 ccgtgagtac gctctttctc aagtagataa acaacgtgaa gattttaaac gtttgggtgt
    24061 ttctggtgac tgggaaaatc catatgtgac cttgactcct gactatgaag cagctcaaat
    24121 tcgtgtattt ggtgagatgg ctaataaggg ttatatctac cgtggtgcta agccagttta
    24181 ctggtcatgg tcatctgagt cagcccttgc tgaagcagag attgaatacc atgacttggt
    24241 ttcaacttcc ctttactatg ccaacaaggt aaaagatggc aaaggagttc tagatacaga
    24301 tacttatatc gttgtctgga caacgactcc atttactatc acagcctctc gtggtttgac
    24361 ggttggtgca gatattgatt acgttttggt tcaacctgct ggtgaagctc gtaagtttgt
    24421 cgttgctgct gaattattga ctagcttgtc tgagaaattt ggctgggctg atgttcaagt
    24481 tttggaaact taccgtggcc aagaactcaa ccacatcgta acagaacacc catgggatac
    24541 agctgtagaa gagttggtaa ttcttggtga ccacgttacg actgactctg gtacaggtat
    24601 tgtccataca gcccctggtt ttggtgagga cgattacaat gttggtattg ctaataatct
    24661 tgaagtcgca gtgactgttg atgaacgtgg tatcatgatg aagaatgctg gtcctgaatt
    24721 tgaaggtcaa ttctatgaaa aggtagttcc aactgttatt gaaaaacttg gtaacctcct
    24781 tcttgcccaa gaagaaatct ctcactcata tccatttgac tggcgtacta agaaaccaat
    24841 catctggcgt gcagttccac aatggtttgc ctcagtttct aaattccgtc aagaaatctt
    24901 ggacgaaatt gaaaaagtga aattccactc agaatggggt aaagtccgtc tttacaatat
    24961 gatccgtgac cgtggtgact gggttatctc tcgtcaacgt gcttggggtg ttccacttcc
    25021 tatcttctac gctgaagatg gtacagctat catggtagct gaaactattg aacacgtagc
    25081 tcaacttttt gaagaacatg gttcaagcat ttggtgggaa cgtgatgcca aagacctctt
    25141 gccagaagga tttactcatc caggttcacc aaacggcgag ttcaaaaaag aaactgatat
    25201 catggacgtt tggtttgact caggttcatc atggaatgga gtggtggtaa accgtcctga
    25261 attgacttac ccagccgacc tttacctaga aggttctgac caataccgtg gttggtttaa
    25321 ctcatcactt atcacatctg ttgccaacca tggcgtagca ccttacaaac aaatcttgtc
    25381 acaaggtttt gcccttgatg gtaaaggtga gaagatgtct aaatctcttg gaaataccat
    25441 tgctccaagc gatgttgaaa aacaattcgg tgctgaaatc ttgcgtctct gggtaacaag
    25501 tgttgactca agcaatgacg tgcgtatctc tatggatatc ttgagccaag tttctgaaac
    25561 ttaccgtaag attcgtaaca ctcttcgttt cttgattgcc aatacatctg actttaaccc
    25621 agctcaagat acagtcgctt acgatgagct tcgttcagtt gataagtaca tgacgattcg
    25681 ctttaaccag cttgtcaaga ccattcgtga tgcttacgca aactttgaat tcttgacaat
    25741 ctacaaggcc ttggtgaact ttatcaacgt tgacttgtca gccttctacc ttgattttgc
    25801 caaagatgtt gtttacattg aaggtgccaa atcactggaa cgccgtcaaa tgcagactgt
    25861 cttctatgac attcttgtca aaatcaccaa actcttgact ccaatccttc ctcacactgc
    25921 ggaagaaatc tggtcatatc ttgagtttga agccgaagac ttcgtccaat tgtcagaatt
    25981 accagaagtt caaacttttg ctaaccaaga agaaatcttg gatacatggg cagccttcat
    26041 ggacttccgt ggacaagcac aaaaagcctt ggaagaagct cgtaatgcaa aagttatcgg
    26101 taaatcactt gaagcacact tgacagttta tccaaatgaa gttgtgaaaa ctctactcga
    26161 agcagtaaac agcaatgtag ctcaactttt gatcgtgtct gagttgacca tcgcagaaga
    26221 accagctccg gaagctgccc ttagcttcga agatgtagtc ttcacagttg aacgcgctgc
    26281 aggtgaagta tgtgaccgtt gccgtcgtat tgacccaaca acagcagaac gtagctacca
    26341 cgcagtcatc tgtgaccact gtgcaagtat cgtagaagaa aactttgcag atgcagtcgc
    26401 agaaggattt gaagagaaat aagattgaaa agtctaggta aaactcaatt tgagaagaaa
    26461 agacaactaa ttttatagtc tattaaacgc attgtatcac gtttttgaac acctgatatg
    26521 atgcgttttt tatttatttt aaaaatttgc gaggtatgac tttttatact caacaagaat
    26581 caaagagaaa cttagcaagc taacagtagt aatataaaat aggaatttga tattagggat
    26641 aagattggta aatagtgtaa tatttttaca acaataaatt tatatagtta tttctggttt
    26701 ctgaaaagta ttatattata ttttatttca tattatacaa atttttattt tataatatca
    26761 gaaaatactt tttttaaaaa gcaaatatga tacaatttta tttgaaaaaa aataaaaaag
    26821 gagattttat tatgaaatca agtggtatcg tcggtcttgt gggaggaatt ttacttctta
    26881 tttgtccttt tgtcttgttg ggaacagcgg taaatacagc tgctacaact cttaatggag
    26941 gagctactgc aggggctttt tcaggtgttg ccttactctt gaatgccttg aagattgcaa
    27001 atcttgttct tggtatcatt gctattgttt actataaagg agataagcat gtaggtgcag
    27061 ctccgtctgt actaatgatt gtttctggtg aagttagtct cattccattc ttaggatggg
    27121 ttggggggat tcttgctatt atcggaggat ctctattcct tgcaacattg aagaaattca
    27181 aatcagaaga ataaaaggta ttttagcatg aaaagaacaa aaaagtttat cggtatagga
    27241 gtagctctat tatctctttc tcttctagtt gcatgtggaa cataaagttc aaagaatact
    27301 tcaacaagta atgatgagaa gacagtagca acatccaata gttcaaaaga aacaatcact
    27361 ttcgatacac cggttgtaac agacgatgcg attgaatcaa tacgcactta tgcagattat
    27421 atagatcttt ataaaaatat ttttgatgat tattttacta aagctgagga aggtttcaaa
    27481 ggcacaacta tggaaaataa tgactcgttt actaaactaa aagagtcaac tcaaaaatta
    27541 ttcgatgcgc agaaaaaaag gttaaataat gaagatagaa tagaaacaac caaaaacaat
    27601 gtgattgcca aacattgtca aacagtcctt tcctttttgg ttttgactag cttttttgtg
    27661 aaaaattgtg taaaatagaa tagataaacg aggggaaacc tcggaaaatt taaaggagaa
    27721 tccatctaat ggtaaaattg gtttttgctc gccacggtga gtctgaatgg aacaaagcta
    27781 accttttcac tggttgggct gatgttgatt tgtctgaaaa aggtacacaa caagcgattg
    27841 acgctggtaa attgatcaaa gaagctggta tcgaatttga ccaagcttac acttcagtat
    27901 tgaaacgtgc tatcaaaaca actaacttgg ctcttgaagc ttctgaccaa ttgtgggttc
    27961 cagttgaaaa atcatggcgc ttgaacgaac gtcactacgg tggtttgact ggtaaaaaca
    28021 aagctgaagc tgctgaacaa tttggtgatg agcaagttca catctggcgt cgttcatacg
    28081 atgtattgcc tccaaatatg gaccgtgatg atgagcactc agctcacaca gaccgtcgtt
    28141 atgcttcact tgacgactca gtcattccag atgctgaaaa cttgaaagtg actttggaac
    28201 gtgctcttcc attctgggaa gataaaatcg ctccagctct taaagatggt aaaaacgtat
    28261 tcgtaggagc tcacggtaac tcaatccgtg cccttgtaaa acacatcaaa ggtttgtcag
    28321 atgatgagat catggacgtg gaaatcccta acttcccacc attggtattc gaattcgacg
    28381 aaaaattgaa cgttgtttct gaatactacc ttggaaaata aaaaattgta agcctaggat
    28441 tgatttctag gctttttatg ttagtatgga agtatgataa ggaataaaaa acaagattat
    28501 gtactggcct acaagcaacc agcttcaacc acttacatgg gttgggaaga agaagcttta
    28561 ccgataggca atggttcttt aggagcaaaa gtatttggcc ttataggggc tgaacggatt
    28621 caatttaatg aaaaaagtct ctggtctgga ggtccacttc ctgatagttc agattatcag
    28681 ggtggaaatc ttcaggatca gtatgttttt ttagctgaga ttcggcaggc tttggagaag
    28741 agagattaca atctggctaa ggaactggct gagcagcacc taattgggcc aaaaacgagt
    28801 caatatggga cctatctgtc ttttggggat attcacattg agttcagcca gcaaggtacg
    28861 actttgtctc aggtgacgga ctatcagaga cagctgaata ttagtaaggc acttgcgacg
    28921 acttcttatg tctataaggg aacgcgattt gaacgtaaag cttttgcgag ttttccagat
    28981 gatctcttga ttcaatgttt tactaaggaa gggttggaaa ctctagattt tactatagaa
    29041 ctatccttga cctgtgattt ggcttctgat ggaaagtatg agcaggaaaa atctgattac
    29101 aaggagtgta agttggatat tactgattct catatcttga tgaagggaag agttaaggat
    29161 aatgatctgc ggtttgctag ttatctagct tgggaaacgg atggagatat tagagtttgg
    29221 tcagataggg ttcagatatc aggagccagt tatgccaatc tcttcttggc cgctaagacg
    29281 gattttgccc aaaatcctgc tagcaattat cgcaagaaac tagatttaga gcaacaggtg
    29341 atagacttgg tggacacagc taaagaaaag ggctataccc aattgaaatc aaggcatatc
    29401 gaggactacc aagccttatt ccagcgtgtt caattggatt tggaagctga tgttgacgca
    29461 tccactacag atgatttgtt aaaaaattat aagccacaag aagggcaggc tttggaggag
    29521 ctgttcttcc agtatggacg gtatttattg attagttcgt ccagagactg cccagatgct
    29581 ctaccagcta acctacaggg agtctggaat gcggtcgaca atcctccttg gaattcggac
    29641 tatcacttaa atgtcaatct gcagatgaat tattggccag cctatgttac caatctccta
    29701 gagacggtct ttccagtcat caactatgta gatgatttgc gtgtctatgg tcgtctagcg
    29761 gctgtaaagt atgcaggaat cgtctctcaa aaaggtgagg aaaatggttg gttggttcat
    29821 actcaagcga ctccctttgg ttggacggca cctggttggg attactattg gggttggtca
    29881 ccagctgcca atgcgtggat gatgcaaacc gtttatgaag cctattcatt ttatagggac
    29941 caagactatc tcagggagaa aatttatccc atgttgaggg aaacggttcg tttttggaat
    30001 gcctttttac ataaggatca gcaggcgcag cgttgggtct cttctccgtc ttattcccca
    30061 gaacatgggc cgatttcgat tggcaatacc tatgaccaat ctctgatttg gcagttattt
    30121 catgatttta ttcaggctgc tcaggaattg ggactggatg aggacttgtt gactgaggtt
    30181 aaggagaagt ctgatttact aaatcctttg caaatcactc aatctggtcg aatcagggag
    30241 tggtatgagg aggaagagca gtattttcaa aatgagaaag tggaggccca gcatcggcac
    30301 gcttcccatc tagtgggact ctattctggc aatctcttta gctacaaggg acaagagtat
    30361 attgaagcgg cgcgtgctag cctcaatgat cgtggagatg gcggcacagg ctggtccaag
    30421 gctaataaga tcaatctctg ggcgcgtttg ggagatggca atcgagccca taaattattg
    30481 gcagagcagt taaagacatc caccttgcca aatctttggt gtagccatcc tccttttcag
    30541 atagatggta attttggtgc tactagtggc atggcagaaa tgttactcca gtctcatgca
    30601 gcttatctgg tacctctagc tgccctacct gatgcttggt caacaggttc tgtttcaggc
    30661 ttaatggcac gtggacattt tgaagtgagc atgagctggg aagataaaaa actcttacag
    30721 ttgaccattt tatcaaggag tggaggagat ttgcgagttt cttatccaga tattgagaag
    30781 agtgtgatta aaatgaatca agaaaaaata aaagcgaaat gcatggggaa agattgtatt
    30841 tcggtggcaa cagcagaagg tgatcttgtt caattttatt tttaagaaga tgttataagg
    30901 cagtaatttg aaacttcctt ttaataagga tttgagaata taggcagttt ccaactagtt
    30961 gaaaaagcgt tataatgata ataggaagta atactcaatg aaaatcaaag agcacaaact
    31021 aggaagctag ccgcaggttg ctcaaaaca
//
LOCUS       DAVBCG010000011        31003 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBCG010000011 DAVBCG010000000
VERSION     DAVBCG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.011"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(297..515)
                     /locus_tag="VQ495_000417"
     CDS             complement(297..515)
                     /locus_tag="VQ495_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2530423.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(761..1039)
                     /locus_tag="VQ495_000418"
     CDS             complement(761..1039)
                     /locus_tag="VQ495_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530424.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1253..2884)
                     /gene="rlmD"
                     /locus_tag="VQ495_000419"
     CDS             complement(1253..2884)
                     /gene="rlmD"
                     /locus_tag="VQ495_000419"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2530425.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2958..3932)
                     /locus_tag="VQ495_000420"
     CDS             complement(2958..3932)
                     /locus_tag="VQ495_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2530426.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4006..4620)
                     /gene="pmp23"
                     /locus_tag="VQ495_000421"
     CDS             complement(4006..4620)
                     /gene="pmp23"
                     /locus_tag="VQ495_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2530427.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4620..5594)
                     /locus_tag="VQ495_000422"
     CDS             complement(4620..5594)
                     /locus_tag="VQ495_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2530428.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5602..6858)
                     /locus_tag="VQ495_000423"
     CDS             complement(5602..6858)
                     /locus_tag="VQ495_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2530429.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6920..7351)
                     /locus_tag="VQ495_000424"
     CDS             complement(6920..7351)
                     /locus_tag="VQ495_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530430.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7353..7955)
                     /locus_tag="VQ495_000425"
     CDS             complement(7353..7955)
                     /locus_tag="VQ495_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2530431.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7939..8778)
                     /gene="prmC"
                     /locus_tag="VQ495_000426"
     CDS             complement(7939..8778)
                     /gene="prmC"
                     /locus_tag="VQ495_000426"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2530432.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8778..9857)
                     /gene="prfA"
                     /locus_tag="VQ495_000427"
     CDS             complement(8778..9857)
                     /gene="prfA"
                     /locus_tag="VQ495_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2530433.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9854..10444)
                     /locus_tag="VQ495_000428"
     CDS             complement(9854..10444)
                     /locus_tag="VQ495_000428"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2530434.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10567..10749
                     /locus_tag="VQ495_000429"
     CDS             10567..10749
                     /locus_tag="VQ495_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2530435.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10886..12259
                     /gene="mnmE"
                     /locus_tag="VQ495_000430"
     CDS             10886..12259
                     /gene="mnmE"
                     /locus_tag="VQ495_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2530436.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12306..13241)
                     /gene="dapA"
                     /locus_tag="VQ495_000431"
     CDS             complement(12306..13241)
                     /gene="dapA"
                     /locus_tag="VQ495_000431"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2530437.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13297..14373)
                     /locus_tag="VQ495_000432"
     CDS             complement(13297..14373)
                     /locus_tag="VQ495_000432"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2530438.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14715..14787)
                     /locus_tag="VQ495_000433"
     tRNA            complement(14715..14787)
                     /locus_tag="VQ495_000433"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14752..14754),aa:Thr,seq:ggt)
     gene            complement(14887..15396)
                     /locus_tag="VQ495_000434"
     CDS             complement(14887..15396)
                     /locus_tag="VQ495_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2530439.1"
                     /translation="MKKLTIRDIADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15394..15490)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15519..15950
                     /locus_tag="VQ495_000435"
     CDS             15519..15950
                     /locus_tag="VQ495_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2530440.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16032..16409
                     /gene="mscL"
                     /locus_tag="VQ495_000436"
     CDS             16032..16409
                     /gene="mscL"
                     /locus_tag="VQ495_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2530441.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16451..17545)
                     /gene="hemH"
                     /locus_tag="VQ495_000437"
     CDS             complement(16451..17545)
                     /gene="hemH"
                     /locus_tag="VQ495_000437"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2530442.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17623..18455)
                     /gene="srtG2"
                     /locus_tag="VQ495_000438"
                     /pseudo
     CDS             complement(17623..18455)
                     /gene="srtG2"
                     /locus_tag="VQ495_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18533..19531)
                     /gene="srtG1"
                     /locus_tag="VQ495_000439"
     CDS             complement(18533..19531)
                     /gene="srtG1"
                     /locus_tag="VQ495_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2530443.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19549..20745)
                     /gene="pitB"
                     /locus_tag="VQ495_000440"
     CDS             complement(19549..20745)
                     /gene="pitB"
                     /locus_tag="VQ495_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2530444.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20790..21341)
                     /gene="sipA"
                     /locus_tag="VQ495_000441"
     CDS             complement(20790..21341)
                     /gene="sipA"
                     /locus_tag="VQ495_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2530445.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21341..23293)
                     /locus_tag="VQ495_000442"
     CDS             complement(21341..23293)
                     /locus_tag="VQ495_000442"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET2530446.1"
                     /translation="MDAGIGTGTNINAGLTEAQRLLQSARAGAKKVVILLSDGEANMY
                     YESNSGRTIYNYYSNPNVGRMIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYI
                     GSNDSITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDVL
                     SKYVQLLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLED
                     DAKYVLKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSRT
                     KIKDYSEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDSY
                     RKVTSPVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYRQ
                     LPSLTVKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQET
                     ALSYLPRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVTG
                     AFANLLQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKIL
                     DLPLNARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSSTL
                     VLGVVLPLGGIFFIILLGHLVVNRRK"
     gene            complement(23423..23926)
                     /locus_tag="VQ495_000443"
     CDS             complement(23423..23926)
                     /locus_tag="VQ495_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET2530447.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            24285..25508
                     /gene="pepT"
                     /locus_tag="VQ495_000444"
     CDS             24285..25508
                     /gene="pepT"
                     /locus_tag="VQ495_000444"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2530448.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25544..26173)
                     /locus_tag="VQ495_000445"
     CDS             complement(25544..26173)
                     /locus_tag="VQ495_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530449.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26138..26359
                     /locus_tag="VQ495_000446"
     CDS             26138..26359
                     /locus_tag="VQ495_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530450.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26630..26978)
                     /locus_tag="VQ495_000447"
                     /pseudo
     CDS             complement(26630..26978)
                     /locus_tag="VQ495_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27083..27643)
                     /locus_tag="VQ495_000448"
                     /pseudo
     CDS             complement(27083..27643)
                     /locus_tag="VQ495_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27869..30988)
                     /gene="phtE"
                     /locus_tag="VQ495_000449"
     CDS             complement(27869..30988)
                     /gene="phtE"
                     /locus_tag="VQ495_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2530451.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         8833 a         6361 c         5686 g        10123 t
ORIGIN      
        1 aaaggatcta gtttttattt ttagttaaat tatgtctata tttttcatta actcatcaat
       61 aaaaatttgt ttgctaatat ctctttcttc tgattttgtt tcaaggatta ttgctacatc
      121 attcatattt ttaggtaaat accaaccatc tggcttatct gaatatccct tgaagcctaa
      181 ttgattaaaa gtagttattt gaccagtacc ttgattgatg ttttcttcaa cttcattaaa
      241 gcctatactt ctcttgcata atccctaact tgatcttctg tccttaaagt cattttctat
      301 tcctcgtcac taaaattatc ttcaaaacta ataatatcat taggagtgca agataaagcc
      361 ttacatattc tttctaaatt cttaaatgta attggtttat cctttcccat ttgtgccata
      421 acatttgttg taagtcctgc catagctatt acatctgttt tctttagtcc tttttttgct
      481 aactgtatcc ataatggttt atagttaagt accataatat ccctcttttt ttcttcatta
      541 aatttattta attatagcat aaatattgat ttcattcaat cttacattga tttagtgatt
      601 tgttttagag aatttgttgt gttattccgt ccttattttg ccataaattg ttttaagtgg
      661 gacctcttat ccttaccatt tttacctcct aattttgagt ataaaaaaga cgatagagtt
      721 tttaatttct atcgtctgag atttacatat taaattttca tcacaaaata agactcttta
      781 aagaatcttt aaattcattg gtatgctgct tacaatatcc attattaaaa tggatactat
      841 aagcagcaca ccaactattt attttttgag ttttaaactt tggtagaagt cttgaactcc
      901 ttatatttac ataactattt gcctctataa ctattatgac acgaaaccaa caagcaagtt
      961 caagcatctc catatcttta ttcaaatgag gactaagata ttcacaaact ttatcataaa
     1021 cctctttatc ttttttcata aaaggggcat acaacttagc aaatttatcc taaaattttt
     1081 tgttgccttt atcaatcatt tcaacgctcc tttaaatata aaaataatcc tatcaatctt
     1141 tatattgagc ctattttcta attgatacct tgcaagaaag gaataatagc tgttcctata
     1201 ccaagtagga atgtaataaa tgcagaaagt catatactgt cattcttttc tattaaatca
     1261 ttttgaagtg tctcaaagca tccatgatgg cttcttcttt ttcaggtgta cactgtggaa
     1321 taatttgttt atccttttta gacttgttgt aatgttctcg taattctatt ccacatttct
     1381 tttttatctg tgcaatatat aatgtcgaaa cttttaattc aaatttattc caaacatatt
     1441 ctttgatttg agcatatgtt gctttgctct ccgcacttgt caaatccatc tcatccagct
     1501 caatttcaac acttatgtgc ttatcgacat cgagtttgga caaaagtgct accgtctcga
     1561 cgtgatgcgt ttgaggaaat agatccaccg gctggacttt cttcaattca tatcccaatt
     1621 cttggtagag tttgatatca cgcgccatgg ttgcgacatt gcaggagata taggcgatgc
     1681 gatcggctcc tgtttgggcg cttgctttga taaagctttc tgtcaagccc ttgcgtggag
     1741 gatcaaccaa gataacggtt ggttgaatac cttccttgag ccatttcttc atggcatttt
     1801 cagccgtgtc acagacatag tgggcattag taatcttgtt caaagaagca ttcttctggc
     1861 tattctcaac cgcttctgga atcagttcaa caccgtagac ttctttgaca tgcttggcga
     1921 ctgataaacc aatggttcca ataccagaat aagcatcaat aaccacgtca tcttttttta
     1981 actctgcaaa gtcaatggct gtttgataga gtttctccgc catttcagta ttgacttggt
     2041 aaaaggctgg gccagcgatt tggaagtcat ttcccaacat ctggtccgta atatagtctt
     2101 gaccataaag agtgcaccac tccttaccaa aaatcgcatt ggtattctgg tcgttgatat
     2161 tttgcatgac agacacaatc tctgggaact gcttgataac ttgttcaatc aattggtcaa
     2221 cacgaaaaac ttttggacga gttgtcacca aaacgaccat gatttgtcct gaatagtgac
     2281 cacgacgcac cacaagattc cgaatcaatc cagactgttc cttttcgtca taaggtttta
     2341 aatcaaaacg acggagcagg tctcgaagag ctactacgac ttggtcaatg acaggatcct
     2401 ggataaagaa atcttcaagg ggcatgaggt tgtgcgaatt cttacggaaa aatcctgttt
     2461 ccaagacacc attcactcga cgaacgggca cctgcgcctt attgcgatac ttgactggat
     2521 gttccatacc aagcgtttca gcaacttcta catctgcaat tccagcaatc ttgtagagac
     2581 tgtccttgac ttgcttggtt ttaaacttga gctgttctgg ataagaaagg tgtcctaaat
     2641 ccgcgattcc tgaacgcagg taagccaaat ctagatcttg attacggtgt agtgactgga
     2701 caaggtattt ttcaactttt ccaaagccaa tctttttatt gaccttgagg acacgcatga
     2761 gaattttttc actcggtaaa gcattctcta caaaaaagac caaaccatct accttggcaa
     2821 ctcctgcccc ttcatgggtc aaatcaacaa tttcaacttc tacaatatca tttttcttta
     2881 acattctttt ctctttctat tggccgacaa aaaagacggc aacgttacga ttaccatcca
     2941 ttataccatg aaatttctta agaaccaaaa ctcttgaaga agttgacaag ggcttgccag
     3001 agggagctta agaagttacc accgtcctct agcgccttat ccgcatcaaa gttaaggttg
     3061 atatttttaa aactgtcgcc agcttgtgat acgatgcttt gtttaaggtc atttagggtt
     3121 ttagtgaaat ctgcattgct gaggatatca ctctttgaga gattcaaggc aaaattgatg
     3181 atgatattga tctggtttcc tgttatgacc tgatcaagtt tgtaattttt taaggtatct
     3241 tcaacaatct tgcggacatc ttcttctgtc agatttccct tgctttcttt agctttggcg
     3301 agtcctgact tgatatcagc tagggcaacg tttaatttat tagcatcata gcctgatttg
     3361 tccttgtttt cagcattgat atctgacaaa gcctttagct cttcttgagc caaatcttta
     3421 ttggcttgcg gcaccttggc tccattagct tctagcgaat agtagatccc agccaaagca
     3481 ctctcacctg taactggaat aggggctgct acagtgattt tggcatgttc catacccagc
     3541 gttactgctg cgtttcggta catatcctga gtcaccttag tgatattttc tggtgtttca
     3601 atcttgacct caagtggcga tttgtcacct agcttttgaa tcttggctga tgaatacaac
     3661 tgtaagctag agtcattggc cacattcatg attttagaat aaacatcagg tgtcatggtc
     3721 ttgagttctt tggtatctgt tgaggcattg tagcccagtt ttttaagagt ttgatttttt
     3781 tggtcttcag atagggagga acctaggaca tattcaggtt ggacataggt ttcatcgata
     3841 actttttgaa catctgttgc tgcatggacg ctattcatag ctgttactgc ccacaagacc
     3901 gcagcactgg tcagaaagag tttctttctc atagggaatt tcctccttta cttttctaga
     3961 gtaatatatc tatcttaaag aaaacttata acaaaaacac ctggtctagc cagatgttga
     4021 aaagagagtg aaacatttga tgatgtaaag gttaagtcgt acctgtctag aataataata
     4081 gtttcctcca tttacataga gttcagcacc gtgaaaaatg gaaatggggt gaatataact
     4141 ataagtcttt ccagtcgtat taccaagcaa gggggcaaca gtctcacgag agtactgttt
     4201 ggctagagcc aaggtatttt ccttgccatt ttgggcgata aaatcgatat aggcaggtcc
     4261 aaaattatag gcttgaacag ctgtccagat atctaccccc ttcttctgcg ccagatagag
     4321 attgcctgtc agagtttgaa tgccttgccg aatgctagag gcattatcat tgatggtgtt
     4381 ggttgaacca cttgcagact cactagactg cataacatcg ccttcttttc cttttgtttc
     4441 agtataaatc atagcaagca caagctcttc gtttgctggg gtgtcttttt cactcaagat
     4501 ttctcgcacc atgggttgat aggtcatgac ttgtttgaca tcttgatgaa cgcggtaagc
     4561 tttatagcca gcaaaaagga agactgctag tacaagcact cttcgaattc gtttaaacat
     4621 tatttacttt ggatatcctc gatatttttg attaagatag agtaggttcc attttccttt
     4681 tggataaact caacagactc ggcgtcttga tagacgttat tgggaacgat gagctcaatt
     4741 ccatttgata aggagagttt ttggttttca aatttcttta attggcgact ggcatcaatt
     4801 tcatcaaatt gaacaggttc tggtacggct tctttgactt ggtcaataaa gctcaaacga
     4861 gccgtcagat tgttgtcaaa aaggtcatta gccaatttct caggtgacaa ttcattgctt
     4921 tcttctaggt tgttgaaaat cgctgatttg accttggatt gaaattgaaa atcatctgtg
     4981 ttaaaagatt cagcaattct ctgggctgtt ttttccagtt ccttgataga ttttttagga
     5041 gaaatcttag gagcgacagc aagaagatta tctgaaaaat agttcaaaaa agtcccgttg
     5101 tacttgattc gtttttcaat caggtgatac ttgcgactct gaagattgac caccaaggcc
     5161 tcgtcagcac ccgttccaaa tccaggcagg ttattctgag tcagcttgat tggattatca
     5221 acttctcctc cgaggtgggt caaggtctcc cgcagggcaa ttcgcaagaa agcgaaatgt
     5281 tctacacctt ctttagaaaa ttgaacaaaa atcaagtcat tggtcttgag attttcagaa
     5341 atgctaaact cctctttcca gagattagcc agcgttactg atgtctccaa caaatcgtct
     5401 gtaatatgat tgaagaaggg attttcttct tcgaaaatcc cagtcttggc ttcatctgaa
     5461 tacacatgtt caattttttt acgtaggtat tcttcgattt ttggagtaat attgagaaac
     5521 ttatctgcta agaacagctc ggtatcatcc ggactgaact ggtgaataat ggctttctta
     5581 atataaatgt ccataaaagt tttattcctc gtataatggg aaggcatctg tcaattcttt
     5641 gactgcactt ctcacttctt ctaatacagc ctcattttct gaattcttaa gggttttaat
     5701 gatgagttca gccactttgc gactttcttc ttcaccaaat ccacgtgcag taatagctgc
     5761 tgctccgata cgaatcccac ttgtcttgaa tggtgacaag gtttcgtaag ggattgaatt
     5821 tttatttagg gtaatattga cttcatccag caagttttga gcaacttttc cgttttctac
     5881 aaccttagtc acatcaacaa ggaagagatg gttttcagtt ccacctgaaa taatacggaa
     5941 atcagggtct tgcaagaaga catctgccat agccttgctg ttcttaatta cattggcagc
     6001 atattccttg aaggctggat ccaaaacttc tttgaaggaa actgccttag ccgccacaac
     6061 atgctctaaa ggaccgccct gaatacctgg gaaaatagct gaattgattt ttttagcaag
     6121 ttcttcgtca ttggtcaaaa tcaaaccacc acgaggtcca cgaagggttt tgtgggtcgt
     6181 tgttgtagtg atatgagcgt atggcactgg gcttgggtga agaccagctg caaccaagcc
     6241 agcgatgtgg gccatatcaa ccataagctt cgcaccgaca gcatctgcga tttcacggaa
     6301 ttttgaaaag tcaataattt gagaataggc tgaagcacca gctacaatca gttttggttt
     6361 tacttcttgg gcttgtttca agatagcatc aaagtctaag agttccgttt caggatcaac
     6421 actataagaa acaaagttgt aggtttgacc agagaagcta acaggagccc catgagtcaa
     6481 atgaccacct gatgccaaat ccattcccat aaccgtatca cctggctcaa tcaaggacat
     6541 gtaagccgca cagttggcct gacttcctga gtgaggttgg acattggcaa atttagcacc
     6601 gaaaatttct tttgcgcgtt caatagcaag agtctctaca acgtctacta catcagttcc
     6661 accataataa cggcgtcctg ggtaaccctc ggcatattta tttgtcaaga tagacccttg
     6721 agctgccata acagccttgg aaactacgtt ttccgaagca attaactcga tattattttg
     6781 ttggcgttct tcttctttgg caatagcatt ccagagatca gcatcatatg ctttaaaatc
     6841 atctttgtca aaaatcatag gtcttctcct ttattgtgtg actagtccat tagtttgatt
     6901 ttacaataag aaaatcaaac taaaagatgc gaataaaccg tttctgcatt ttatcacaag
     6961 tatagccaac tttttcataa aatgcatgag cacccagacg atgattggca gaatttaagc
     7021 ggataaaccc ataaccacat cttttggctt cttgttccaa cccttgtagt aaacttttac
     7081 cgataccttg accttgcgct tgaggtgaaa ctgctaaagc taagatatta aatcctgctt
     7141 tggaatagag tgattcgtaa acttcagcgt ggacatatcc aagtaagaca tgattagctg
     7201 catcctcata gccaagtagg aaatgatggg aatcctgaga cagtctagct agttggctag
     7261 ccgtttcctc tggactaaaa gtataaccca aagcctcttg gttgatgtca catatcgctt
     7321 tcacatctgt ttcttgcaaa tctcttagca tttcaagcct cctcaaaaga aatctctggc
     7381 aaccgagcaa gaatatcttc tcgtttaatt gcgccttggc gtaagatttt caccttgtct
     7441 ccagacaaat ccacaatagt tgaatcctgt ccagttagaa aagcatcgtc ttccagaccc
     7501 agaacctctt ggtcaaaatc cttcagaatt tgttcaaagg ttacaccact tgcctgacct
     7561 gagatattgg cagacggccc aatcaaggga cctgtctctc gaattaaatc cagtgtgata
     7621 gggtgactgg gcatccgaaa tccaatagtt gcaaggtcag aatttaccca atagggaact
     7681 cggtcattgg cttcgagaat aatggtcaag ggacctggca aaaaggtctc tacaagtttt
     7741 tgtagataag ttggctgatt cttagaaaag tgcaagatat cctcgaaaga ggcgatattg
     7801 agattgagtg ccttatctct aggacgacgt ttgagttggt aaacatggtc aactgctttt
     7861 tcatctaagg ccttggcaaa aagaccataa acagtctctg taggtagaac gacagctcca
     7921 ccattttcca actcttgtct aatcctgtcc atcatcaacc acaaccatcc tattttgacc
     7981 aaattggtct ttgagtgttc gaactttttt ttcaggaaga tgttttctaa aaagttcggg
     8041 aacactttga ccttgcttgt atccaatttc aaggtaaatc ttaccaccat ccttgagata
     8101 gtcttttgca tcttccgcaa ttctacagta aatagctagg ccatcctcgt ctgcaaagag
     8161 agctagatga ggctccgaat gcaaaacatt caagcctacc tctgactcat cttcacgaga
     8221 gatatagggt ggattggata caattatatc atatttttca gaaatttctg caaaacaatc
     8281 agatttttta aaaaatatat taagattttg atcgctagca ttctctgatg caagctctaa
     8341 ggcctcttga gaaacatctg ctgctgtcac tgaccaatct ggtctatttt ttgctagagc
     8401 gagagcaata gctccactac ctgttccaat atccagaact gagagattcg tctcaagatt
     8461 ttcagttaga ataagctcca ctaactcctc tgtttctgga cgaggaatca aaacccgttc
     8521 atccaccttt aaatgcattc cataaaaatc tgcctgtcca atgatgtact gagctggctt
     8581 gtgagctgct agttgctggt aaatatcttc tacaaattgt ttttcttcct ctgttgtcac
     8641 ctcctgctgg agggcaaaaa taaagtctgt aaaagataga tttttcagac tacgatagac
     8701 aaaagagagg ctttccgctt cctctccttg tcttatcaac tcttcttcaa aatttgaaaa
     8761 taattgagct aatttcatta tttgtttaat tcttctagtt tttgtgtttg gtcataaagc
     8821 accaaggcat ccacaacttc gtccaattta ccagacaaaa tcgtatctag tttttggagg
     8881 gtcaagccga tacggtggtc tgtgacacgg ttttgaggga agttataagt tcggatccgt
     8941 tctgaacggt caccagtacc gattgtcgac ttacgctcag cgtcttgttc atcctgagca
     9001 atctgagcaa agtggtcagc gacgcgtgca cggataatct tcatagcctt ctcacggttc
     9061 ttctgctggg tacgttcttc ctgcatctca accttgatat tggttggcaa gtgaacgata
     9121 cgaacggcag tcgcaacctt attgacgttc tgtccaccag cacctgatgc gtggtaaata
     9181 tcaacacgaa gatcttttgg atcgatatcg tattctactt cttcgacttc aggcatgaca
     9241 agaactgtgg cagttgaggt atgaacacga ccttggcttt ctgtcacggg gacacgctgc
     9301 acacggtggg cacctgattc gtacttaagc ttagagtata cagactgacc tgaaaccata
     9361 gcaaccactt ctttaaaccc accgacacca ttcatagagg cttccatgac ttcaaagcgc
     9421 cagccttggg cttccgcata cttttggtac atagttagca aatctccagc gaaaagtgcc
     9481 gcttcgtctc caccagctgc tccacggatt tcaaggatga tattcttgtc atcgtttgga
     9541 tcctttggaa ggagcaaaat tttcagtttt tcttcatatt cttctttttc agccttggca
     9601 tctttgagtt cttgcttggc catttcttcc aagtccgcat ctccgcctga ttccttaatc
     9661 atctcttcgg catcgacgat attttgaagg acttgtttat actcacggta ggctattacg
     9721 gtatcacgat tggaagcttc ttcttttgaa agctccataa aacgcttggt gtctgaaacg
     9781 acatcagggt cactcagcaa ttctcctagt tcttcataac ggtcttctac agcttgtagt
     9841 tgatcataga tgttcatttt tcttcattct ccttattgat ttcaggggca aaataatgtt
     9901 tacggcaaac cgagatatag gtttcattac caccaatttg gatctgttct ccatcgtaaa
     9961 cgggcagtcc atcctgtgtt cgcaacacca tggtcgcctt tttcttgcaa tactgacaga
    10021 tggtcttgat ttcgtcaatc ttgtctgcta aaagcaagag atatttggaa ccttcgaaca
    10081 attcattgcg aaagtcattt ttcaagccaa aagccatgac gggtatgtct aactcgtcca
    10141 caacacgagc taggtcataa acatggtggc gtttgagaaa ctgggcttca tcgaccaaca
    10201 cacagtaagg tttttctggt aggcctcgga tatagccaaa gatatccgtt gtttcctcaa
    10261 tcgcaagggc agggcgtttc atgccaattc gactcgacac atagccaaca ccgtcacgcg
    10321 tatccagagc tgaagtcata atcacaacac cttttccttg ctcctcatag ttataggcca
    10381 ccttgagaat ctcgattgtc tttccagagt tcatggtccc ataacgataa tacaactgtg
    10441 ccatgtttct tgcttcacgt ccatttctaa atttttgcta cattctagta tatcataatt
    10501 ttcttaagct ttaaacggca aaatgtggta aaatggaaga aatcaaaact agtggaggaa
    10561 gctattatgc catttgtacg catcgattta tttgaaggac gcacgctcga gcaaaagaaa
    10621 gctcttgcta aggaagtaac ggaagcagtt gtccgcaaca ctggagcccc tcaatctgct
    10681 gtccatgtca tcatcaacga catgccagaa ggaacttact tcccacaagg ggaaatgcgt
    10741 actaaataag ctagcttaag cagaattgct taggcttttt caatctccaa gtagcattca
    10801 ttgaagaaat atcctaaatt tgttacaatt tgaaaagaaa cttggagaat ttccaagaaa
    10861 agagctatta attaaaggaa acattatgat tacacgtgaa tttgatacca tcgctgctat
    10921 ctctactcca ctaggtgaag gggctattgg tattgtccgc ctgagcggaa cagacagttt
    10981 tgctattgcg caaaagattt ttaaaggaaa agacttgaac aaggttgcca gccacactct
    11041 caactacggt cacattattg atcctctgac tggtaaagtc atggacgagg ttatggttgg
    11101 ggctatgaag tctccaaaga ccttcactcg tgaggatatt atcgagatta acacccacgg
    11161 tggtattgcg gtgaccaatg agattctcca gctagctatc cgtgaaggag ctcggttggc
    11221 agaacctggt gaatttacca aacgtgcctt tttaaacggt cgcgtagatt tgacacaggc
    11281 cgaggcggtg atggatatca tccgcgccaa gacagacaag gccatgaaca tcgcggtcaa
    11341 acaacttgat ggttcccttt ctgatcttat caataatatc cgtcaagaaa tccttaacac
    11401 acttgcccaa gttgaggtca atatcgacta tcctgagtat gacgatgttg aggaagccac
    11461 tactgctgtt gtccgagaga agacaatgga gtttgagcaa ttactaacca agctccttag
    11521 gacagcacgt cgtggtaaaa tccttcgtga aggaatttca acggctatca ttggacgtcc
    11581 caacgttggg aaatcaagcc ttctcaacaa cctcttgcgt gaggacaagg ctatcgtaac
    11641 cgatatcgct gggacaacac gagatgtcat cgaagagtac gtcaacatca atggtgtacc
    11701 tctcaaattg attgatacag ctggtattcg tgaaacggat gatatagtgg aacaaatcgg
    11761 tgttgagcgt tcgaaaaaag ccctcaagga agccaacttg gttctactag tgctaaatgc
    11821 cagtgaacca ctgactgcgc aagacagaca acttcttgaa attagccaag ataccaatcg
    11881 cattattcta cttaataaaa ccgacctgcc agaaacgatt gaaacttcga aactacctga
    11941 agacgttatc cgtatttcag tccttaaaaa ccaaaacatc gacaagattg aagagcgaat
    12001 caacaacctc ttctttgaaa atgctggctt gattgagcaa gatgctactt acttgtcaaa
    12061 cgcccgtcac atttccctga ttgaaaaagc agttgaaagc ctacaagccg ttaatcaagg
    12121 tcttgagctg gggatgccag ttgatttgct tcaagttgac ttgactcgta cttgggaaat
    12181 cctcggagaa atcactgggg atgctgctcc agatgaactc atcacccaac tctttagcca
    12241 attctgttta ggaaaataag aaaaatccat gatccttcat tcggtcatgg attttattgt
    12301 ctttattagt aatctggtct taagacccct gttacagttg ccttagttgc ttcgtagtcg
    12361 ccatctacga caaccttgat aatgcgtttg gcatcttctt ctggtgctgg aacaagaggt
    12421 agacgagtgg gtccagcttc aaatcccata tagttaagaa ttgccttaac tggagcagga
    12481 cttggataag agaagagagc attaacctta ggaatgaatt tacgctgaat tgctgcggct
    12541 ttcttcatat cgctttctgc aatggcagta aacatctcgt gcatttcatc cccatttgta
    12601 tgagaggcaa cagaaataac cccatccgcc ccaaggttca tggcatggaa agcatctcca
    12661 tcctcacctg tataaatcaa gaactcttca ggcttgtgct caatcaagta agccatatta
    12721 gccaagctag tacattcttt gacaccgata atatttggat ggtcagccaa gcgaagcatg
    12781 gtttctggag tcaattcgac aactacacgc cctggaatgt tatagataat aattggtagg
    12841 tcagaagcat ctgcaatagt cttaaagtgc tgatacatcc cttcttgaga aggtttgttg
    12901 tagtaaggaa caatagcaag cccagctgcg aaaccaccaa attccgctac ttctttgaca
    12961 aactcaatag agtcacgcgt atcattagta cctacacccg caatcaaagg aacgcgtcca
    13021 ttgacaacct tttgtacagc cgcaaacaac tccaactcct catcgtgggt caaagttgga
    13081 ctctcagcag tcgttcctgc gagaagaatt ccatccgtat gatgggccaa taaatgctca
    13141 atcaaggctg gaatagcatc aaagttaata gaaccatcct catgaaaggg ggtaataaag
    13201 gctgtaatga ttttacattc ttttaaatct tgataagaca tgaaaacacc tctctatttc
    13261 aaagaaggag ttcatctgaa ctcctaaacg atatgactat tttaattcaa atttcaattc
    13321 ggctgttgga cgaaccaatc cacgttcatg aagagtttca gcaatctgaa ctgagttcca
    13381 agcagcacct ttgagaaggt tatctgaaac aacccacatg tgaattcctt tttctgcatc
    13441 caagtcttta cggatacgac caacaaaggt atcacgcgaa ccaactgcat tgatagcttg
    13501 aggatagatt tgatgagcta catcatcttc aagaacagca cctgggaagg ctgcgatagc
    13561 tgcttttact tcttcgattg gagccacttc ttttgtttcg atataaacag actcagagtg
    13621 agctgacaag actggaatac gcacacatgt tgcagatact gcaatgctat catcttccat
    13681 aattttctta gtttccttgg tcatcttcat ctcttcgtac gtgtaatcat tatcagtgaa
    13741 aacatcaatt tgtggaagag cgttaaaggc gataggataa tgtttcttgt caccacctga
    13801 aggcaaaatt tccgcatgca aatcacatgg tttcacacca tcattcaaga cttcacgaag
    13861 ttcacgttgt gtctcaagaa ttgctcccat accagcacct gaaacggctt gataagttga
    13921 aacaatgata cggtccaagc cccatttttg gcgaaccggc tcaagagcca ccatcatttg
    13981 gattgttgaa caattagggc aggcaatgat tccgttgtgg gcatcaagtg catgagcatt
    14041 gacctctgga acaaccaaag gaacatctgg attttgacgg aaataagatg tattatccac
    14101 tactaccacg ccagctttta ctgcgtatgg tgcatactta gctgatgtag aactacctgc
    14161 tgaaaagaga gcaatatcaa ctccttcaaa agctgtttca gtcgtttctt caattgtaat
    14221 atcttgatct ttaaatttca atgacttacc tgctgaacgt gcagaagcaa ggtaacggat
    14281 tttatcgatt ggaagtgttg attcttccaa catttttatc atctgagcac cgacagcacc
    14341 tgtcgcgccg actacagcaa ctgtatatcc cataaataac ctctttcgga attttctaaa
    14401 aatttctata atagaagtat tatactactt tttccatgaa ttgcaaagct ttttcatcat
    14461 tttttggaaa ataaaaatcc cagtcgctag actagggact aagaggcatc ttcatgtgat
    14521 gagcaggttc acacaactca tcaaggtccg ctcctgcgtt atgacctcct tatgctcaat
    14581 agtagtccga agactaccta tcgactcatc gcatattcta tattactaga gatacttgat
    14641 tttgtcaata agaaattttt tcgatcgagt ataacaaaaa actctcgtat tcaagagttt
    14701 ccctatacat atcatgctaa ttgccgggat cgaaccggcg acctcatcct taccatggat
    14761 gcgctctgcc aactgagcta aatcagctta cttaagaagt atactatatt cagaaaaact
    14821 tgtcaagttt ccttagaaat tttccatagg aaaaagccag gtaagaacta cctagcttat
    14881 cttcttctat ttacttaaat caattcgacg accaatttta ccaataataa tcgccccaat
    14941 aatcaatgaa gcaaattcac cgattcctgt tgtaaaccaa gtaaggaaga atggtaattc
    15001 tgctacaata tttaactctg cagcgatagt aaacatggaa attgaaaaga ggattgaaaa
    15061 gaagaaatga tcttttcgaa tcaatccgtt aaagaggtaa tccttgctat attttgcaaa
    15121 aagccaaaca cctaaactga ggaatactaa ggttgaacca ccaccaacaa agacatctag
    15181 cagtccgaag ctaaagaaat tagcaatcat acaaccgatg gtaactccga tgatgtactt
    15241 aggattgtaa aaagccataa agttcatcat ttctgaaata cgaaactgat aagcaccata
    15301 gctgatggca tttaggggcg gtgtgacagt caaaacgacg tagatagcag cgacgattgc
    15361 aatatctgca atatcacgaa tagttaattt tttcatcttt tctcctttgg cggttgcccg
    15421 cgtgtaatat gcttggtgaa agaagctaag caccaagggt tgctttattc aaccctacta
    15481 gtatagcaca atagcctgct ttatgctata cttaacacat gaaaattcct attcaaaaat
    15541 tttttaacaa tgaaatctta gcctatctct tttttggcct tgcgacaact ctagtttcga
    15601 ttctctcacg actagtcatt tatcaactaa gtcatcagga actcctggct actgccctag
    15661 caaatattat cggtatccta tttgccttta tcacaaatga tacgattgta tttaagcaaa
    15721 aaaagaaaaa tagacttatc cgtttagtga aattttctct tgctcgcctt tctacttttc
    15781 tgttagactt gtttttaact tttctctttg taacccaatt tcctcacatt atagggcaat
    15841 tcgtaactga aaacattgat aagataaatg ctattgaaac aataattgca caaatattca
    15901 ttattatcat caattatata ttgagcaagg cctatatttt tagaaaataa tgctccattt
    15961 atgagccttt taattgcctt ctataatgat ttaggataca atgaaacacg aaacattgaa
    16021 aggaaaacag tatgttaaaa aatctaaaat cgttcttgct tcgaggaaat gttattgacc
    16081 ttgctgtcgg tgttgtaatt gcctctgctt ttggtgctat cgttacttca cttgtaaacg
    16141 acattatcac tcctcttatt ttaaatcccg ctttgaaagc tgctaaagtt gaacgtatcg
    16201 ctcaactttc ttggcatgga gtcggctatg gtaacttctt aagtgctatt atcaatttta
    16261 tctttgtggg taccgccctc ttctttatta tcaagggcat tgaaaaagca cagaaactga
    16321 ctggcataaa gaaagaaaaa actgccgaaa aaaaaccaac cgaattggaa gtccttcaag
    16381 aaataaaagc tctccttgag aaaaaataat cgattaaaag gatctagcgc aaagctaaat
    16441 ccttttttct ttactctttg ggtaacttgc ctgctttttc aagcattttt ttgatcagat
    16501 aaggcatctt aacattctcc cgcccttttt tctcaatcag ttttttcaca aattctggca
    16561 tttgtaaatc ttcagattca gctaaaatgt ttttcgtctc gtctgaagga actaactcat
    16621 caaaggtctc ttcttctggg agaaattcct taaactcttg attttcattg actctaactg
    16681 tattgaccaa agcatcaatc aaacgagaat ctgtatttgg cattggtgga cgatggtagt
    16741 ttaccccaaa ttcctggcat aagtcataac attctacatc attgtcaaac aagacctcaa
    16801 tatgctcact gatgaaacta ataggtacaa aaatataatg gtctggatgt tctgtctgtt
    16861 ctctgagata ctccaaaaca tctggtttaa tccatggaat accaatatca ctctcactct
    16921 gccaagtgtt ggtatattgt tctgaactca agcccagttt ttcagcgact agcttgctat
    16981 tttcgaaaat ttggtctata taaggatcac caaagtccaa ggcaaaaatg ggcacactat
    17041 gggctgaaaa gatgacttta aagctgtctt gctttacttc ttcttttaaa atcttagcaa
    17101 tttcatctgc ccaatagttt aagagcactt cttcctgata ccagtcctta atgaccaaaa
    17161 attgaatttg tttgctttct aaaaattttt catagcccat gacagagtag aaagaataat
    17221 ggggctccaa aatcaagcag atacattctt caacaccatc agtttccatc tgcttgatca
    17281 cgtctggaat aaagggacga gaaaatttgt tggcaaagta gataccgtac tcctccccta
    17341 aacgttcttt gactaaattg acctcctcac gtgtaatccg ctgcaaaggc gtcccaccga
    17401 tacgaatata gttgtcatag agagtctgga tttcgtggtc ttggggtcta actccacgac
    17461 gaatgtttgt aaagaattct gctactcctt caaaactaat ctcctctgga gatccaaagg
    17521 tcatcattaa tatggctttt ttcatgttat catccttatg atattatttt ctctatccat
    17581 tctatcatga aagaaaaaaa tagtaaacgt ttcctacgtc tactatttct tttgctcttt
    17641 tgttttttta tctcgtctta taattaggat gataaggaca agaagtaaga ggacgataat
    17701 aaggagtaga aaggctagaa tgtaaatcca atgactggga attccgtgat cgaaataccg
    17761 tgttcctact gcttccccac tttcatcctg tgctactttc accggtgcat ccgttatttt
    17821 agctaaaagg atatgtcgtc cattagttat gttaaggaaa catctcgctt gtacttggct
    17881 ttacttagca gatattgata gtaagcctgg tgttcctcat ccttggaact caaggttcga
    17941 taaatctccg tgtcataggc atccacttct aaaatcccaa aaatttcaag accacgttct
    18001 cgaccattgt agtagataga accgtagcga tgctggtcga agaattcctt atcagcaaat
    18061 ttcttaatct caccgaacat agcccctgaa gccatagagt gtccgtagat gatcgtatta
    18121 aaatcagtaa agttgggatt aaagcgatag tcgagaaaaa ttgatcccat tatcgtataa
    18181 tcgccaaagg cattgcggtc aaggtatttg acattatcct gactttgtag aagcggatag
    18241 tctaccttgg tcccatcaag gctaatccaa ccgcaaacat ctttgttcaa gtgctgtagg
    18301 gtttcaaaac ctgtgctcga agcatccttt tcaataggat tatagtgatc atagtcactt
    18361 gcttctacta ctctctgcca actaaaaaga gctacaaaaa cacagaagaa taccagaaat
    18421 acgtttttaa atcgcgctct tttttgaacc gtcatcagca ctcctttcta tgcgagattt
    18481 cttaaaagga aaggaggcgg gacaaaaccg cctcttttct attcaccctt agtcagtaaa
    18541 agggtctgct tcttgcattt tttttactct ctcttctacg acgtaggatc aagtagagga
    18601 caaggagtaa gaaaatcaag agcaagaaca atacaaacaa gataaggtac caaatccata
    18661 gtgggattga tgagaggaaa cgtccaagag acgagtcatc aaagacactg tatggaaatg
    18721 gttttgattt ttttgtatgg aaagtatttt tagggacggt gtctgtaatc tttgcgacta
    18781 cgatatgacg accattggtc acatcgagaa aacaggtact gagtaggatg atacgatcac
    18841 tcggtgagag tgagatatcc cgcttgtgca tggcgactga gagcaggtgg tctagatagg
    18901 cctgctggcg gtcctcaccc tgtattcctg gatcatagat gttaaagtca taggcatcaa
    18961 cctccaacat ctcaaagatc tcgagccctt tttccacacc attgtagtat atactaccgt
    19021 aacgatgctg gtcaaaaaat tcctgatcag caaacttagc cacatcacca aacatgaccc
    19081 cattttctac gtggtgcccg tagataatgg tattaaagtc ttcgaactta ggattatttc
    19141 gtgcatcgag aaagatagcg cctgtcgctg caaactcacc tttggagtcc ttgttgagat
    19201 acttttcatt gtccttggct tggactaagg gatagtcgat attggtgcca tagacattga
    19261 tccaaccgag gacttcggga ttgagttctt ggagtttgct gaagcctgaa aaactggcca
    19321 gctcatcctg ttgcgtcgtg acaggtcgat aagcttcgta ctcacttgac gaagctaagg
    19381 agtagacttg gttggaatcc caaagggcgt agaaaccaaa gagaaagatc aaagctgcaa
    19441 aaatgagcag caagtgattg atcaccccat ccacagcaag aacgagacgc ctagacagac
    19501 tcttttttgg tttctcacgc ttggttttca tcatctgccc ccagccgttt aggcttctct
    19561 acgtttttta gacaagacaa caaatccagc caaaccaaga ccaatcatca aaatgaatgg
    19621 aaggttatca atcaagagac cagtaggagt aacgtcgtcg attttgttag taactttgtt
    19681 gtcgtttttc ttttcaccta tgaggatatc tgttaatgta aaatctacat ttttttgtcc
    19741 ttgagatgtt tgttctgcac catttgtagt ataaattgat gaatctgtat aacctgctac
    19801 tccagtttca gttactgtat atttagaacc tgctggtagt tgttcaactg taaatgtttc
    19861 accatcttta agagtaaaat tgtactcaac tgtatctgct ggggtcacta ctgccaacac
    19921 ctctgactta gatccatgtt ttactataat attcgtaaca ggggtttctg ctgttttatt
    19981 tgtacttgga agttgaatct tgatatggaa ttggaaatct cttgttttat cccctgaagc
    20041 tccatcaact tttttagtaa ttgttaatgc tttcgcattg gggtctactt gactcggatc
    20101 atcaggatta ggctccttac ttgcgtcttt ggtatatgtg ttatcaaata aattataaac
    20161 gccttgttca cttggttcta ctttaggggc gttagattta ttattttcct taaagtatac
    20221 tgatgaaata tagacaccac catttacttt attcttaaca ataacgtgca tttcatatgt
    20281 acgtttgtcg tatctcatgg catcaattgt tttaccattt ttggtaatcg cctgccatcc
    20341 agtttttgct tccgctacat cataaacgta ttcaccagca tggccataag caactcccga
    20401 aaaaatgtct tctgttgctt tttgaatctt atcagcagtt gggtgatcat ttttattgta
    20461 ttctacattt ctatctggaa ttgcaactcc ggtttgatat ggcgcaccgt tagaagattg
    20521 tcctgttttt tctgtaaact taaatgtaaa tgtcgctgtt ggtgttgtta ttccctctgc
    20581 aatattcaac gttttattaa tcttcacaac agcattattt tcaccatttg ctttggttat
    20641 tgctgaatta tcatccgcaa aagtcgggac acctctgaat acggacaaag cggttatagc
    20701 tgcaatgctt gcagtgaata gctttttaaa gaatgttttt ttcatgatta tttcttttgt
    20761 ttttttattt tcttttttca ttatgatcat caaaagcctc gtcttctaat gactgttaca
    20821 actttgcctt gagtatcctg tatagggatg gttccaaaag cacgactgtc tacagccgtt
    20881 gttcgattgt ccccgaggac aaaaagttgt cctgctggga ctttcatcgg gaaggttgcc
    20941 ccttccttat agagcagcgt ttctttgtag atatcttgct cttgttgagg agaaccgttg
    21001 atgatgagac cattttcgtt tatatcgatt gtacttcctt cggttgctat gacacgcgcc
    21061 actctttcct tacctttata actatagact agcaaatcac caatcgaata gcgtttatcc
    21121 aacctataat agacgaccaa gtcgccatct tttaaggcgg gcttcatgcc atcgtcattg
    21181 tatcttaata atccaaagac gaaaaagtaa aggatggcta gtacaaatcc aaccatcaga
    21241 agtttactga ctaagtaaag gatatctccc cagacactcg gcggagactt atcccgattg
    21301 acttgtgtta ctggtttctt atgtttcttt ttaagcagca tcacttcctc ctattcacca
    21361 caaggtgacc aagtaagatg ataaagaaga tccctcctag aggaagaacg actccaagca
    21421 cgagagtgct actcaagaag tcaattcccg tttcaggtac gctgttttta ttattcgtga
    21481 ctgtcgcaga cttatttgca tcaagagttc cttctttttt atcataggac acctgatacc
    21541 cacgagacga acttgcttct tcttcgatac tataacgagc atttagagga aggtcgagaa
    21601 tcttgatggt tttatctttc tttagatcaa ctgtcgcctt accattggtg aattgtagcg
    21661 ttgttttttg attatttact attgcactat acgatccatt caatggttta ttttgcgcat
    21721 cctttacgtt aatggtaatc ttaaaggatt gcagaagatt agcaaaagca cctgtaactt
    21781 tttttgtgac tgaaagtgtc ggaagtctgt ggttggtgac gattgtttga acatcctctg
    21841 acaaagtccc ctcttgtttt tcataggtga catgatatcc cgttctagag ttctctactt
    21901 cttccacctt atagtgggta tcacgaggca gataactgag ggcagtttcc tgactatttt
    21961 tgagagaaat ctgtgctttc ccgttagtca ctgttgtatt cccaaacttg ccgttaatcg
    22021 gcttgccatc cttatctgaa aaggtaacat taattgtgaa agatttatta ggtgattgtt
    22081 caccttctag gattttcttt acggtgagac ttggaagctg tcggtaaata actttgaagg
    22141 atggtttctc tttcgtacct gtcttttgaa cttcgactgt gtatcccgga gcgtctggtg
    22201 ctttcagttc gtagtcatat cccttagcta cctttgcaaa actagtattt tcagtaaaac
    22261 tattcgtttg aactggacta gtgacctttc gataggaatc ctttatactt ccatctttct
    22321 tttggtttaa gtttaattcg acactaggtg gacgatttgc tgatggattt gattttccat
    22381 ccacaccttt ccactcaatc tctacaggaa ccgtcaatgg atctgacggc ttgaaagtgg
    22441 gcttctcaga atagtctttt attttggtac gtgaggtccc aataccatag gaataggcaa
    22501 cttttgcccc cttgttggag tagagtttag tagcatcggc ttcttcggca tcatcactag
    22561 taagtgtctt atcacccgca atcgcatcaa atgcctcttg gctagaggtg acagtaaact
    22621 tgagaacgta cttggcgtca tcctccaaag tgtaactcgg attaaacttg gctgttactt
    22681 ccactaatcc ctgttcgttc ttcttagttt caatcgtaac ttgattgtca ttcagttctt
    22741 gctcgttacc atccttgatt ttgacgacac gaaggtgtga agcatcacca ggtaacagct
    22801 gcacgtactt ggacaagaca tctgatatag ttacatggtg tacgcctaga ggtagaattt
    22861 tatctgtgat ttctttgaat ttttgttgca agtcattttc attattggca gaaaatattt
    22921 cgttggggat tccagaatta taaccactga tatatccttt aagagatgtg atactatcgt
    22981 ttgaacctat atatctgaat ttgattgaat aaaagccatc tatttttgga gctattaaac
    23041 tagatatatt cagcattcct ctctctaaac cagaggtaaa ccaatatgga gtatcaatca
    23101 tacgtcccac atttggatta gaataatagt tatatattgt tctcccacta ttagactcgt
    23161 aatacatatt agcttcgcca tctgacagca gaataactac tttttttgcc ccagcccttg
    23221 cactttgcaa caatctttga gcttcagtta acccagcatt tatatttgtc ccagtgccaa
    23281 tgcccgcgtc cataccataa gaacgactag agtcagcaat tgttaacgaa ctaacaatat
    23341 tttttgagct attagaatcc gtgctccaag atactaaagt tttagcatca tcatatgaag
    23401 agatatttga ataccaacca gctcaatttg gccaagttcc atattgataa ctcagataat
    23461 attcattata ataacggtca ttatactgat tatcaatctt tccggcaaaa ccaactatag
    23521 acagacggtt ttgggaatta gaaagaattg tatcaatgag cccctgacga ccattcacac
    23581 ctttcagtgt atgttttagt gcttcaatcc tactaatgta tcgtctacta gagtaagacc
    23641 acacatctcg ctcttccata ctccctgaaa gatcggcaac caagactaca tccaagggtt
    23701 ctgactgggt ctccgttccc agtttagatg tgatatccag tgacaactca tactggtctt
    23761 tttgccctga tatcggagta atcgttttgt gcagagttgt ctgaggttca gttattgaat
    23821 ctgcacgtaa agaattaatt ggtgcacaca aaaaaagaat agcgagaaag ataactaaac
    23881 tactggtaaa atattgaatc ttcttaaaat catattgaac gttcatgcta tctcccttgc
    23941 tagaatatgt tttaatctga tactatataa atctgagact tccataagat aattgtataa
    24001 taatattaac ttcgtgtcaa ttaatgtgtt tgaaaacatg ttattataaa ttacaaaaaa
    24061 gtatgattct tctttgtttt tcaacttttt ctgtggtaaa atggaaaaaa tagtaaaaaa
    24121 tttcataaaa taaagatgca tatatttatt ctaatcgctc gaaaatttct tttgaaagtc
    24181 ttctgaattt gaattattat attttttcaa aattcataaa atttctcaaa tcatattttt
    24241 ccgaagtttt gtattctatt ttatgataaa aaaggagttt ttttatgact tatccaaatc
    24301 tcttggaccg cttcttaacc tacgttaagg tcaacacgcg ctctgatgaa cactctacta
    24361 ctactccaag tacacagagt caggttgact tcgcaacaaa tgtcctaatt cctgaaatga
    24421 aacgtgttgg actgcaaaat gtttactatc taccgaatgg ttttgctatt ggaaccttgc
    24481 cagccaacga tccgtcttta acacgtaaga ttggttttat atcgcacatg gatactgctg
    24541 attttaatgc tgaaggagtc aatccacagg taattgaaaa ctacgatggt ggtgtgattg
    24601 aactagggaa ttctggtttc aaactcgatc cagccgactt caagagtctt gaaaaatatc
    24661 caagacaaac actcatcaca acagatggaa caaccttgct aggtgctgat gacaagtcag
    24721 gaattgctga aattatgaca gccattgaat atctaactgc tcatcctgaa attaagcact
    24781 gtgagattcg tgttggtttt ggtccagatg aagaaatcgg tgttggtgcc aataaatttg
    24841 atgcagaaga ttttgatgtg gattttgcct acactgttga tggtggtcca ctaggtgaac
    24901 ttcagtacga gactttctca gccgctggtg ctgaattgca tttccaaggc cgtaatgtcc
    24961 accctggtac tgccaaaggg cagatggtca atgcccttca gctagcaatt gattttcata
    25021 atcaacttcc agaaaatgac cgacctgagt taactgaagg ttaccaaggt ttttaccatc
    25081 taatggatgt gacaggtagt gttgaggagg tgcgtgcaag ctacatcatt cgtgattttg
    25141 aaaaagatgc ctttgaagcg cgtaaagcat ccatgcaatc tatcgctgat aagatgaatg
    25201 aagaacttgg gagcgaccgt gtcactctca acttgacaga ccagtactac aatatgaaag
    25261 aagtcattga aaaagatatg actccaatta ccattgctaa agccgttatg gaagatctag
    25321 gtatcacgcc tattatcgaa ccaatccggg gtggaacaga cggctctaag atttccttta
    25381 tgggaatccc aactccgaat atctttgcag gtggcgaaaa tatgcacgga cgttttgaat
    25441 acgttagcct tcagactatg gaacgtgcag ttgataccat cattggcatc gtagcttata
    25501 aaggctaaaa agacgaggtg gctcagctac ttcgcctttc tttttattct actggttttt
    25561 cttgatttcc agtagttgta gaagattctg ttgtttcatt ttctgaagtt gattcagcag
    25621 gtttagaatc tcttgtattg cttggtttgt tttcgtcgct agcagtttca atgttagatt
    25681 ctgcagttgc gtttggttgg ttctcagcac tggtgttatc actatttgct tcagcatttc
    25741 ttgctggact tgtttcttca cttgcgctaa cttttgactg gatttgatga ttcaaaacta
    25801 gaatagcttt tgtcgattca agtaaagctg ttttgtcttt actattagca gaaagttgat
    25861 ctaataatgc atccacctta tcaaagtccg catcagatcc attattactt tctaaataag
    25921 agtgaagcga catgagaata tcgtagagtt tttgatagag tacaagtgtc tgagaatctt
    25981 gctcagcatt ttccttttct tgttgaaggg cgctagcgat acgagtcaaa acatctttta
    26041 cctgactgtt tacttcatcc aagtctgcat cagccttgtt tgtggcagct tttagatttt
    26101 ctacttcttc tgccaaggat tgtctgattc cttcttcatg gatttgttcc aagagttgat
    26161 ttgccttgtt caaaagactt tctacttctt ccttgctatc tgtcgcagat tattggttgc
    26221 tatctaccat gtactcctaa aacaggagag ttataatcca agattacaag gccttacaga
    26281 aataagaaat ccagataaga caatgttcgt ccaagacgct attcgcttcg cacagcagca
    26341 cggattcaat atgctttaat tttaaagttt aggtgtcaag acctcttttt agtgtgccca
    26401 aaatttagag aagtaatcaa tcaactaact tttatttttt tcaaactttc agtaaactga
    26461 cctaaagcta actcaatctg tctttgtaga tgcttctgct atcagctaga agttgatcta
    26521 cttttgccaa gactgccttc tcatcaaaag ttccaggttg atagttggat tgcagggatg
    26581 gaatcttgtt tttcaaagcc gcttcatatc ccttagtttg aaccttgatg tagtgattgt
    26641 ggtcgccatg aggaatcaca aaaccttctg aatcttcact tataattcga ttggcatcaa
    26701 aaaccataac catcttcttc ctcatggtgg acatgtagtg acggattact taatacagaa
    26761 ctagaagaac ttcctacctc ttccgtgtta gagtgtgatg ggggattgtt aagagatgac
    26821 ttaggaatat agtgatagtg ttccccatgt cttactatat aagcatcacc tatatctctg
    26881 acaatatcat tagggttaaa gacatatgtg gctgctaatt cacctgccga caagtcactc
    26941 tcaggaatga aatgatagtg accaccatgt ggtactatta gtagattgaa atagaatatg
    27001 agcaaattga taaggggatt ttaaagtaat ttctaacaat gatttagaaa ctatgatgtg
    27061 ctattctaaa ttcaactcac tatatataac catcatcggt agtataacgt ccctgtaatt
    27121 ttgctacaga tacttctgca ctagctcctt tatcgtcttt accatgttct tgtttttggc
    27181 gattgatttc atcttttgtt cgtacatttt ctgcatgagc ttgatcttta aggtaaacat
    27241 aatactttcc atctacctta ataatatatc ctcccttaac ctaactgaca atatcttgat
    27301 ctttcggctg atagttgggg gctttcatta atggctcttc actaaagagc gcatcaaaag
    27361 gaactttacc attatagtag tgataatgat cgccatgaga agttacataa ccttgatctg
    27421 taatcttaat aacaatttgt tttgcttgaa ttccttcttt ttgactaacc tagtctggag
    27481 tcaaattttc agtcttctta gtgtctttat tactgtttac atatgaaaca cgatttttat
    27541 ctgtattggc ctgttagcta tgttggttca gagcataaac acacagactt aaggaaagga
    27601 taacaacaga tccagctgct atatatttct ttttaaattt cataattacc tcatttctat
    27661 aattatttat atgatgtctt cattattaaa tgattataga aattaattaa ccaattaatt
    27721 aactagtaaa tattccacct ctttttaagt tgtatgtcaa gaaattttat atattaataa
    27781 taaaatgaaa ttctcccaaa gtcagagttt tatttctaac ttttgagaga acttcatttt
    27841 tgattcagac tttttctact gctattcctt acgctatgag atcagataaa ttctttttta
    27901 tcacttctcc acttggcaat cttaattcaa tcgttccatc catattgaat ataacactat
    27961 ctaagcctaa tccgtaacta gctgtaaatt tttctaattt ttcttgtaca ggatctactg
    28021 ctggagcttc ctctaatgct ggatctaaca tagggtcact ccccacattc ccttctggat
    28081 tcaacattcc attatccgtt gagttttctg gttttacagg tttttcgttt ggtgcctctg
    28141 gtaaagaatc tgctggttta ttttctgttg gttggttctc aactgttcca gtagatactt
    28201 ttccattttc agatggttta ttttcaccat ttccttgagg tgcttctcct gtaaaatctg
    28261 ccatattctt tttaatgact tctcccgatg gtaaatataa ttcaattgtt ccgtccatat
    28321 taaacaagac attttctagc ttcatcccat aactttcagc aaattttgct actttttctt
    28381 gtacaggatc cactgtagga acttcttcta acgttgaatt actagtacta ttcccagttt
    28441 cagaaagttt ttctttttct accttctcac tagtctttgg ttcttctacc ttttcatcaa
    28501 gttttgagtt ttcttgtgct ttattccttt taaattgtgg tagaatactt ggtttatcag
    28561 tttgattttc tttttccaag ataggtactt ccacaatata agtcgattga ttgtccaaat
    28621 aagcatttgc catgaaggtt acaggaattt tatttccggc cgttctggtt gttccttggt
    28681 ttaatttcgg aatcggtaat ttgatttcac caactttata gttattttct aaataagcat
    28741 ttccatgaaa ttcatcaaac actctgacta aagcatcagt tcctttaggc actgcaaatt
    28801 gagggttcac tcttaaataa gtatcccctg catggaaagg atagaaaatc gtttgactgg
    28861 ccattttgta agctaaagag gttggaactg taaatgtacc atcataactt acttctggat
    28921 aatcttttga agcgatagta tacttaaatg tttgtcctgg taaataaggt tgatctaatt
    28981 caaagtttgc aatattccct actccttctc caaatacttt accagatact ttctccaata
    29041 cttttccatc tggtgttatt aattttacta gcatattgat acctaatttt ttctccaatt
    29101 caggcggaaa actaaaagaa acgcgttttt gaccattggc tagagtaaag ttttgattat
    29161 taaacgtact attttttaac aaattaacaa cattcgttaa ttcttctcca gtataaactt
    29221 tattcccttc ttttttagca actccttctt cgggtttaaa cagttcatag ttactgtgag
    29281 aatgaccaat tccaaccggt ttatgttcat caatcggatc tgcatgatgg tgatctccat
    29341 gcggataaat aatcgcattt ttttctttat tcacgacaat actttcacgt ttgacaccat
    29401 attgtttcat aatgccagca attttttctt cgattttttt atctaaatct ttcatttctt
    29461 tggcattact tggataatcc tgttcatgag atgacaaaga atctaatcca ttatgactag
    29521 ttttaacttc ctctaaatgt ttttgcgcag ccttaatttg ctcttctgtc aagtccttct
    29581 tgaagaaata atgattgtgg tctccgtgac tcatgacaaa acctgattca tcttcagcga
    29641 taatacgatt agcatcaaat ccgtatccat cttcttcatg tttctcatgt gaagttcctg
    29701 gattgattgg aagagatgga gaaggtgttg ctagactatt gtttggaaga gtcggttgcc
    29761 caatttgatt tgattttgga atgtaatgga aatgatcacc atgtcttaca atataagctg
    29821 tagccgtttc ttcaacgata tcttttggat taaaaatata accatcagat gctgaagaga
    29881 gctccttact tgtcgttaaa gaagaaggat tgcttgaaag actgcctaga ctagacacta
    29941 cttcattagg ttttgcattt gtagaaactg tagaaccagt tccactgata ggcaccattc
    30001 tggcaatctt ttcttctaag gcagaaagct tgctgtaagg aataaagtgg taatggtcgc
    30061 catgcggaat cgcaactcca tttggtgtac gactgataat cttagcaggg tcaaagacca
    30121 ggccatctga ttcactgtaa cgttgggcgc taggtgaatc atagagttcc ttcaaaagac
    30181 tctggagatt ttcagattta tttgctggct tgctagttga tccttttgct acagattgcg
    30241 tgttattgtc actagctgtt gaagaatagc ttaactgact cggttgcata ttttttccag
    30301 ccagatgtgc tttagctgct gctaattcac tagcagataa atcgcttttg ggaatgtagt
    30361 gatagtgacc tccatgagga acgatataag cattacccgt atcttcgata atatcagctg
    30421 gattaaagac ataaccatca tttgtcgtat atcgtccctg agaccttgct acagcaacat
    30481 tagagttaac cttctcatta tctttgacat gttcttgttt ttgacgattg atttcatctt
    30541 tagttcgaac attatcagca tgagctgcat ctttcaggta gacataatat tttccatcga
    30601 ccttgatgat ataaccaccc ttgacttcat tgacaatatc agcgtcttta agttgatagt
    30661 ttggatcctt catcaagagt tcttcactaa agagggcatc ataaggaact ttcccattat
    30721 agtaatgata gtggtcaccg tgtgacgtta catagccctg atctgtaatt ttgattacaa
    30781 tttgctcggc ctgaattcct tctttctggc taatctggtc tggtgtcaag ttttcacttt
    30841 tctgacttga ctggctgcca tctacataag agacacgatt attgtcctta ttttcctgcg
    30901 aacgatgctg gtttagtgca taggcacata gactcaagga tacgataaca gctgatccag
    30961 ctgctatata ttttttactg aatttcataa atccctcatt tca
//
LOCUS       DAVBCG010000012        30917 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBCG010000012 DAVBCG010000000
VERSION     DAVBCG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.012"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..174
                     /locus_tag="VQ495_000450"
     CDS             <1..174
                     /locus_tag="VQ495_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET2530452.1"
                     /translation="FFGILKSEMFYGYEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLK
                     GLSPVQYRTKSFG"
     gene            complement(302..1324)
                     /gene="cbpF"
                     /locus_tag="VQ495_000451"
                     /pseudo
     CDS             complement(302..1324)
                     /gene="cbpF"
                     /locus_tag="VQ495_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
     gene            complement(1343..2164)
                     /gene="cbpG"
                     /locus_tag="VQ495_000452"
     CDS             complement(1343..2164)
                     /gene="cbpG"
                     /locus_tag="VQ495_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HET2530453.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNRKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2245..2484)
                     /locus_tag="VQ495_000453"
     CDS             complement(2245..2484)
                     /locus_tag="VQ495_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530454.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2634..2900)
                     /locus_tag="VQ495_000454"
                     /pseudo
     CDS             complement(2634..2900)
                     /locus_tag="VQ495_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2925..3236)
                     /locus_tag="VQ495_000455"
     CDS             complement(2925..3236)
                     /locus_tag="VQ495_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2530455.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(3161..3403)
                     /locus_tag="VQ495_000456"
                     /pseudo
     CDS             complement(3161..3403)
                     /locus_tag="VQ495_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3404..4036)
                     /locus_tag="VQ495_000457"
     CDS             complement(3404..4036)
                     /locus_tag="VQ495_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2530456.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(4050..5045)
                     /locus_tag="VQ495_000458"
     CDS             complement(4050..5045)
                     /locus_tag="VQ495_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2530457.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5042..5740)
                     /locus_tag="VQ495_000459"
     CDS             complement(5042..5740)
                     /locus_tag="VQ495_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2530458.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5818..6828)
                     /locus_tag="VQ495_000460"
     CDS             complement(5818..6828)
                     /locus_tag="VQ495_000460"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2530459.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6812..7819)
                     /locus_tag="VQ495_000461"
     CDS             complement(6812..7819)
                     /locus_tag="VQ495_000461"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2530460.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRSFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7806..8759)
                     /gene="mvaD"
                     /locus_tag="VQ495_000462"
     CDS             complement(7806..8759)
                     /gene="mvaD"
                     /locus_tag="VQ495_000462"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2530461.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVIADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YSVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8741..9619)
                     /gene="mvk"
                     /locus_tag="VQ495_000463"
     CDS             complement(8741..9619)
                     /gene="mvk"
                     /locus_tag="VQ495_000463"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2530462.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9741..10070)
                     /locus_tag="VQ495_000464"
     CDS             complement(9741..10070)
                     /locus_tag="VQ495_000464"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2530463.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
     gene            10727..11063
                     /locus_tag="VQ495_000465"
                     /pseudo
     CDS             10727..11063
                     /locus_tag="VQ495_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
     gene            10963..11550
                     /locus_tag="VQ495_000466"
     CDS             10963..11550
                     /locus_tag="VQ495_000466"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530464.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            11593..12825
                     /locus_tag="VQ495_000467"
     CDS             11593..12825
                     /locus_tag="VQ495_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2530465.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(12943..13899)
                     /gene="cbpC"
                     /locus_tag="VQ495_000468"
     CDS             complement(12943..13899)
                     /gene="cbpC"
                     /locus_tag="VQ495_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2530466.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(13997..14686)
                     /locus_tag="VQ495_000469"
     CDS             complement(13997..14686)
                     /locus_tag="VQ495_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2530467.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(14698..16122)
                     /gene="gndA"
                     /locus_tag="VQ495_000470"
     CDS             complement(14698..16122)
                     /gene="gndA"
                     /locus_tag="VQ495_000470"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2530468.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(16198..17592)
                     /gene="mapZ"
                     /locus_tag="VQ495_000471"
     CDS             complement(16198..17592)
                     /gene="mapZ"
                     /locus_tag="VQ495_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2530469.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(17605..18762)
                     /locus_tag="VQ495_000472"
     CDS             complement(17605..18762)
                     /locus_tag="VQ495_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2530470.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(18847..19227)
                     /gene="rnpB"
                     /locus_tag="VQ495_000473"
     ncRNA           complement(18847..19227)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ495_000473"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(19248..19577)
                     /gene="gpsB"
                     /locus_tag="VQ495_000474"
     CDS             complement(19248..19577)
                     /gene="gpsB"
                     /locus_tag="VQ495_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2530471.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(19647..20174)
                     /locus_tag="VQ495_000475"
     CDS             complement(19647..20174)
                     /locus_tag="VQ495_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2530472.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            20240..20836
                     /gene="recU"
                     /locus_tag="VQ495_000476"
     CDS             20240..20836
                     /gene="recU"
                     /locus_tag="VQ495_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2530473.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLTVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            20833..22992
                     /locus_tag="VQ495_000477"
     CDS             20833..22992
                     /locus_tag="VQ495_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2530474.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(23159..28462)
                     /locus_tag="VQ495_000478"
     CDS             complement(23159..28462)
                     /locus_tag="VQ495_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2530475.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(28763..30745)
                     /locus_tag="VQ495_000479"
     CDS             complement(28763..30745)
                     /locus_tag="VQ495_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2530476.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         8874 a         7016 c         5531 g         9496 t
ORIGIN      
        1 ttctttggga ttctgaaatc ggaaatgttt tatggctatg agaaaacatt taaatcactt
       61 aatcaattgg aacaagctat tatagactat attgattatt acaacaacaa gagaattaag
      121 gtaaaactaa aaggacttag tcctgtgcag tacagaacta aatcctttgg ataaattatt
      181 tgtctaactt ttggggggca gtacagaaaa accttgatac tatgcgtttt ttgattgtga
      241 agacttttat ccacctatta tacaaagtta agtatccatc acagtttaca attagccttc
      301 attacttaac ccattcacca ttatagttta agtagtaacc acctactgtg gtattaacag
      361 ctaaagcacc tgaatcatag gcatagtacc agttaccatt gacttggaac cagcctgttt
      421 tcatatctcc attacttgca tttagatagt accaagtcga accttcctga taccaaccag
      481 ttgccatagc tcctgatgaa cggagatagt accacttgtt accaaggtat tgccagccag
      541 ttctcatatc accattttca gcatctagat agtaccaagt tgagccttcc ttataccagc
      601 cagttgccat agctcctgac gaatggaggt agtaccatct attacctaga aattgccaac
      661 cagtttgcat aatgccagtt gttggatcta gatagtacca tggagctgct tttagcttct
      721 cttcatcata cgtaagaggg gaatccttaa cccaaccaca tgctagctct ccaaccgcca
      781 atctgttgat gcgcgaatca aagccaccat ccttctgtaa ataataccaa tgaccctctt
      841 cgtaaatcca accagttttt aaagtatgat aactacgctg atcttcaaaa taatagactc
      901 atttctctgc tgggctatca tattcttccc catgatgttt gttggtattc gtagcagttt
      961 ttgcttctaa aacttgcttg ccaacaaatt cttgtaatac accatcttga ccaaaataaa
     1021 accaatctgg tctaagagaa atctctattc ttggagaagg accaatcgta acccccttgt
     1081 gtggagcagg tatatattgc cagccgacaa ccatctctcc ggataaagga tcaaaataat
     1141 agtacttccc atctatcact ctccagtagg tttcttttag atcccccttt ttgtagtagg
     1201 ttctaccatt ttcttggaca aactgccatc cttcagaatc atctgcaaat actgtactgg
     1261 tccctagcaa accgaagaaa aatgtggcta gtgcgacttg catcattttt ttcaaaagct
     1321 tcattttata tatcctccaa cattaaatcc actcaccaga tgaagcgaag ttataaactt
     1381 taccatcgat agtttggcta cctgtaacca ttgctccatt tgaattgaga taataccatt
     1441 ttctatttac tttttgccaa tctgtaagca tcttaccgga actgtctaaa taataccaag
     1501 tatcatttat ctcctgccaa cccgtttgtt tatcatgttg tctataatag taccaactac
     1561 catttatttt cttccatcct tcaagagagt aacctttaag aaccgaataa ataaatggca
     1621 aatttcgttc atttaattta actgcactgt taatttgatt agctccatct cctaaagtat
     1681 gcactcctac tacacggtga ctagcatcat aaactgtaga tccactagac ccctctaaag
     1741 tatcaacttg gtaatccaag aacatgccat catcacttaa aacttgtttt ttatctgtat
     1801 acatttgatg aattttaaaa ttatatgatg gatagcctgt gatagtcaca gttattcctg
     1861 tcaaattttt ttgactagta ggaagaccca aagtccctaa ttttgcacca atgggctctt
     1921 ctagaattaa taaagccaag tcatattccc ttgcatcctt agaatttaaa tttctaaatt
     1981 ccttcaaata acgaacttcc tttactttga tctttccaaa tagttcttga cttggactaa
     2041 cagccggaag aacataaata tcatccgctt ctttcccata gtcatgtctg tagtagttgt
     2101 gagccgctgt aattaacaca ttatctttaa taaaattcgc acttcccctc ccttctacat
     2161 tcattccatc ggctactcca taatacttag ataaaaccat agctgaagtc gaatacggat
     2221 actgtaaagt attatcaatt ttaatcaaat catcattacc gataatactt ctgattgctt
     2281 ttggtagtat gaaccatacg ttggtgaaat ctcagataat gaagaatcat tagactctgg
     2341 acctttttct agtgtctcac ttacctcata ttcttcacct gtactagaaa taacactcaa
     2401 agcagatact gtcgataact ggctagccaa taaagtactc gcaataattg aaatacccaa
     2461 ttttttataa acagttttct tcattattgt atcctcctaa tgtaattata gcgtactatt
     2521 ctaaatttct taatctacta tagaatcaag aaatctacca ccttctttaa ataccctcca
     2581 ttatcacata aacaggtaaa cttttcaatt aatgactgcg cttttcaatc acgctagagg
     2641 tacttacttg cttctttgat actaagttca gccattcttt ccttgttttc atcaataaag
     2701 catgttaccc aagttggatt cgttttggag tagtctcgca gaatccagcc aatggcttta
     2761 ttgataaaaa attctgtttg gttcaagtta tgaagtagaa tcttttccat taattgagta
     2821 ttggtcttct cttttcttaa caactggtgg tcaatagcga cacgtctcag ccagatatta
     2881 tctgataggc tccattttat actcaatgaa aatcaaagag caaactagga agctagccgc
     2941 agattgctca aaacactgtt ttgaggttgc agatagagct gacgtggttt gaagagattt
     3001 tcgaagagta ttaagattat ttcttctagt tcagggtgtt catacaccaa actccctact
     3061 actcgatcta ggatatctac cgtgtcccac caggattttg tcacgactaa ctgctctagc
     3121 ttaggcaaat cggtttcctt tagataagac tgcattgctt tcaaatagtt agcagccaca
     3181 tattggtatt ttctaggctc cttttcccag caagtgtctg caaaatccca atcgataatc
     3241 tttgtttttt tcgcttctag taaatatttt tatagagttt atttctttca ggcaccgcaa
     3301 tacctagaaa agaaaattga tggcgcatat aggcttccat gggccttgct tttttagagt
     3361 cttttgctgc ttctagctcc tcaagtaaat ctgctaaact catctaaaac tcctcttgcc
     3421 ccaccaaatg gtgctgaaag gcatagacag ccgcctgggt acgatcgctg acttcaagtt
     3481 tggcaagaat attggacacg tgggtcttga ccgtcttgag agagataaag aggtcatctg
     3541 cgatgcgctg attttcgtag cccttggcga tgagttggag aacatctcgc tcacgcgcag
     3601 tcaattcttc atgaagttcc atatgattgc ggtggtattc aaccttcttg ctaacctctt
     3661 gctcaatggc cagctcgcca gcagctacct tactgacggc atgaagcaat tcatctgcac
     3721 tagaagtctt gagcatatag cctttggcac cagcatctaa gactggcatg attttttcat
     3781 tgtccaaata agaggtcaca atcaaaatct tggcttcagg ccattcttta aggattgcta
     3841 aggtcgcgtc aattccattc atctcaggca tgacaatatc catgacaatg acatctggac
     3901 gcagttccaa ggccaagtca atcccttgag acccgttgga cgcctcaccc acaacttcta
     3961 catcgtcttg gaggtcaaag tagcttttca agcccaatcg gaccatttca tggtcatcta
     4021 ctagtaaaat tttcatcttt actcctttat cattccttat ctaacagggg aatacggata
     4081 tcaaccgcca gcccttgctt gggagctgtc aagagttgaa ctgttccagc catatcttca
     4141 acccgctcct tgatatttcg cagtccataa ctcaagtcgt ctaagctccc taactggaaa
     4201 ccaatcccat tgtccaccac cttcagttgc aattcaacat ctgtctgata gaggtagaca
     4261 tctaggcaag atgcctgggc atggcggagg gtattgctaa tcaactcttg caggatacgg
     4321 aagatatgct cctcgatttt cttaggcaat ttcgtcatat tctgcttgag actaacccta
     4381 agatcactct tgtcctcaag ctcttttaaa agaatttgaa tcccttctat caagctcttc
     4441 tgctccagtt caactggtcg caaatgcaag agcaaaaccc gcaaatcctt ctgggctgtt
     4501 tctaaaatag ctgtgacact ctgcaactgg gtctgcatct tttctctatc caatttcaaa
     4561 gcctgctgac tgatacccga taaaatcatg tgggccgcaa acaactcctg actgactgta
     4621 tcgtgcaaat cccgagcaat tcgcttccgt tccttctcga tgatttcctc ttcctgagca
     4681 aggctatgat tttcagcttt ttgaagagcc tctgtcaaaa ggttaagttt acctgataag
     4741 gacttgaaac tggcatccaa atctggatct acaacctgaa ccacttcttg ccctgccaat
     4801 aaacgcttga gattagcctg catttttctt agagaaagct cttcgatccc tcgccaaaac
     4861 agggctaaga gacaggtcat ggacatgctg aaaaccaaca ataaaaagaa aaatttttct
     4921 gttttttcga catcgtgcaa aaagatagac cagtcaaaat caagtatttc cagcaagcta
     4981 tgggagaaaa aaaagacaaa caggaaggag gtgagagcga taatgacata ggcttgtttt
     5041 ttcatcctct aaccacctca acatcaccaa tcatagtggt caagaaaatc ttgacactct
     5101 tgttactctt gagatagtct tttgtttctt gatgatagtg ttcattgcgg agggctcgct
     5161 tgggctggtt gaaaaaagtc aaatccccat agagacagtt aacgctgaga ctgacttcca
     5221 catctacagg tacgatgatt ttggtcgttc ctaccatctt tctgaggata atgacattgt
     5281 catgattggt taagatgact ctctccagat gaatagtgtc cttgcccatg aagcgaaaga
     5341 gattgatatc atcaaattgg caagtctggt agcttgaaaa atgatgaaga tttccaaacc
     5401 aacgattttt ctccttctta accgtcacaa cctcttcaaa aaccaaattg gtctgctctt
     5461 tttcctggtt catcatcggg taaagaagaa agagactata gataaccgca acaaaaatag
     5521 ctagaatcac aaaaggattg agcataacga tgaaaaagaa gagaatggtt gccgctacta
     5581 aaagaagatt attcccctct ttaccagtat agtagcgaat caaaagcaaa aagaggaata
     5641 gtatcagcag aaaacgcgaa aaatgctctg ataccatcaa aatcagagct cctgtcagaa
     5701 gacaggcttc gataaataaa aagattttaa attttctcat aggttcatcc tctcccttct
     5761 attttatcac aattcaaaaa agtcacctca gtctgaggat ggaaaaaaag gcgctggtta
     5821 cgcctttttc atctgatcct ttgcttcttt taattttcca taaagaagat agtctacttt
     5881 ttgtagatct gctatggtgg cacagttaag ggaacacata atcaagcgta gatctgcttt
     5941 ccagccttgg acaatgccaa tcacttcttc aactgtgtag gtttcaacca attccagaac
     6001 ggttcgtgac aatcccacag ccttagcacc aaaaaccaag cacttaatca tatccagcgg
     6061 attccgaacc cctccactaa ccaagagttc gaccttatct ttccattctt gggcattgag
     6121 aagggcctgc atggtagact gaccccattg attgaggtaa tcacgctggc cactacgacg
     6181 gttttcgata taggcaaagc tggtgccacc acgacccgat aggtccactg tacgaacacc
     6241 gaattcatag gctctttcga ttgtcttggc atccattcca aagcccactt ccttgaggac
     6301 aataggaacg ggaatttgct tgctataatc tgctagatgc gattgccagc ttctaaactt
     6361 cctttctccc tcgggcatga gtaattcctg catgacattg acatgcactt gcaatagaac
     6421 aggattcatc tcttctacag tctgaagtcc taactcgaca ggtttgtcca atccaatatt
     6481 ggttccaagg aggagattgg gatgactaga cttgacagaa aaagaatcat ccgttggatt
     6541 tttgagggct gcgctataag aacccgttac aaataaaata ccacaggatt ccgccacctg
     6601 agccagcttt tgattgattt ctcttccctt attacttcca ccagtcatgg cattgatata
     6661 aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa agatcgattt catccagatt
     6721 gtaaagaggc aaggaagaat gaatcagctc cacctcatca aagctattat aggaactttt
     6781 ctgctcaagg gcatagagga tatgctcgtc cttacgattt gtcgtcatgt cctatccttt
     6841 cttgatataa gagctcaatc cccagatcgg cccaacgatt ttttaaggtt ttggttgatt
     6901 gcgcatcaaa actcagggcg atgccacagt caccaccacc agcaccacta ctcttggcaa
     6961 cggcctgcaa atcttgactg gcttctttca actgtctaag caaaggcgtg taaatatctg
     7021 tactcaagcc ttctaaaagc ttgctggcta cttctacttg ctcgataatc ttttctgatt
     7081 tcccctgttc caaggcttcg accaaagaaa ccaccgtttc ttttgaggaa cttaaaaaat
     7141 tttgattgat attttgcttg atttgctgga ccatgtgact cgatacagcc acttccttgg
     7201 tccatcccac taagaaatca cattctaaag ttggtttcac ttgtgaaatt gaaaagcccc
     7261 aatcacgctc cagaactgtc gccaagtttt cttcttctaa ccaagcagcc accttctggc
     7321 gatcaaatga ctggtagaga accaaatcct ctgccacaat acaggcaagg tcgcccatgg
     7381 aaccattgtc tcctcgcttg agcaagacag cgctagtcag cttgaacaag agctcctgat
     7441 caacagaaac atcatacaga gccagtaaag ccttgacaac caagacaacg acgctgccac
     7501 tagaacctag accaaacttt ttcccttctc gttccatttt gccacagatt tctagggaaa
     7561 aagatcttaa attctgaccg cgaacagcga ggaagtctcc catcaaagca atcgtttctt
     7621 gaatcaagct gtagtcagga ttgggcgtta agtccactgc gaaatcaaac atatctgaat
     7681 agatacggta gctgtcagaa aaagcaatct cagccctcat atagatggga atatccttta
     7741 tcaaagctaa ctgccctggc tctaaaatag catattcacc tgcccaatag agttttccgc
     7801 aagttttaac agcaatcatc ttgactcaaa tcctttgttt ttgacacaat caagcgataa
     7861 cgctgaccga aaatttctga caaatgctcc aagtctttct cctgacaaaa gaccttaaca
     7921 ttgggaccag catccatggt aaagtagcag gcctctcctt tctcacgaag ctggcgaaca
     7981 aagtccatag cctcataaga ggcatccgtc agataagaaa aggctggact agcagtcttt
     8041 gtcgtagcat gcatagccag ggcatttttc tccgttaatt ctccaatctt ggcaaaatca
     8101 ttttccttga gataaatcag catatcctga tagtccttct cagactgacg aacccagtcg
     8161 tcaaaagtcg tcgaggtttc cacacaaagt ttcatcccgt cacggctaga gattggtttt
     8221 ttcttgtcct ctagcaccaa cataatcata gctagtttca agtctgtctc tacagagtaa
     8281 atttctccac tatccttatc ccaggctcct agcggtccat aaaaactccg agaagaagag
     8341 cctgaggcaa acttggcttc ctgcgctaac tgacttctat ccaatccaag cttgaaataa
     8401 gcattacaag ccttgaccag ggcggacaaa ccactagaac ttgaggacag gcccgctgca
     8461 gtaggcatat tgttttgagt atcgatacgg acaaagccct caccagctgg acgataacgg
     8521 tcaataatct tactcatctt ggcatgctcg acctcatttt gtagctgacc attgatgtaa
     8581 aattcgtcag ctattacatt ggctggtaaa ggcgacaagg tcgtctctgt atacatattt
     8641 tccaaagtta gagaaatact gctagtagca ggcaccatct ctttttcttt tttctttccc
     8701 caatatttga taatagcaat atttgcgtag gaacgtactg tcacaggctc tctatccatg
     8761 tctgaacagc tcctttctct tctaatcttt ctgctagttc ttgtgcgtgt gtcaaattgg
     8821 ttaccaaggc tatgatacaa cctcctagcc caccaccgct catcttggca cccagagcac
     8881 catggctaag agtcgtttca accaaaaagt ctgcctcagg gctactgact ccaatttctt
     8941 ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc ttcagcatct ttttgtgaaa
     9001 tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg caaaaacggt agggcatcct
     9061 tgcccttatt ttgaaccact tggatggctt cacgagtatg accataaaca cccgtatcgg
     9121 caatcaccaa ataggcggat aaatccatct caagttctgt aaatcctacg ttcttgataa
     9181 agcgaatagg ttggtcactg agacaggtct tagcatccaa accactagga ttcatatggg
     9241 caatcatctc agctcgattg accaagattt ctagtacatc atgaggcaga tcagcctgat
     9301 agtagtcaaa taccgcacga atggccgcta tgctgatagc cgctgacgaa cccatccccc
     9361 gtttctcagg gatagccgag tcaatctcac aacgaatgca ggcttctgtg atatccaaat
     9421 actccagtga ggcataaacc gccatggaca aggtatcctc ctcataaagg cgccaaggac
     9481 tctctgcagg aactacctta caggtcacct ccacctccaa aagaggcagg gaaatggcag
     9541 gataaccgta aacgaccgca tgttccccta ttaaaattat cttactatgt gcctgaccga
     9601 caccaacttt ttttgtcatt ttttcctttt actagacgaa aaaacgtctt atttttcata
     9661 caagtattaa ttctttccta tctattttat tatattttca caaaaaaagc gattgtttcc
     9721 attcacaatc gcttctttca ttattgaacc cattcgccat tatagttgac agaatagcca
     9781 tctacggtcg tattcactgc caaggcacct gagctataag cgtagtacca tctgccattg
     9841 acctggaacc aacctgtcgt catagaacga cgaaagaaac tccataccgt taagtaaaga
     9901 ggaaagtcgt gagggatcat gcgccattga caacctgttt tagtgacgta caaagtctca
     9961 ttaacaagta ctcgtttcgg ccatttatag gtgcggtgtt tggagaaata gggttcaatc
    10021 ttagcccatt cttgatcgtt taaatcagta tcatatgctt tgcgtgtcat aactctagtt
    10081 taacattttt tgtgaataca agttctaaat aatcgaccac gaaaatttct taagtggaaa
    10141 acgccttatg aagtatgcta cgggaaactt atgcacttaa tttgacaatt caagatgtaa
    10201 aaatatatac tatagtagat tgaaactaga atagtacacc tctacttcta aaacattttt
    10261 agaaatcgat ttgactgtcc tgatcgattt gtcctgttat tattttacta taatatttga
    10321 caagttatcc taaaagtatt attatgttgt tgtgttatag attgattgaa tctaactaaa
    10381 ggatcctatt caattactag aactatcaca tactcaaggt cagctcacag atgagcaact
    10441 attttggtta caatgtctac taaatttaag tcaaacaaat aatttagtca aaattaaaaa
    10501 aatagaggaa cataaatatg attacaaaac agaatgtaat agtgttctac gatttttact
    10561 agataaaact gtaaattctg aaggaaggat cacttcttca acagaatttg gaaatttcgt
    10621 aagtaattta tcattccaac acggaatagc tggactactg tttcctctaa ataaattgta
    10681 ccccccagaa ctggattcta aaatactctc tatcatcaag aaggcagtga caattagaac
    10741 gacacacata tgaatatcaa tactcactgc tatttggtga tgcaggctat ctatggttac
    10801 tcctacattt attttctatc agtaaaaatc aatactatct acaattagca aacgtcaccg
    10861 ctaagaaatt aatagagaat tatgatactc tagaagaaat agactttgca ttgggaaaat
    10921 ctggtgtcct attatcatta ataaaatact atcaatttac caatgacaat actcttaaaa
    10981 ctttcatcca caatagtata tggggaaatt tatcattatt tcctacaaag agatacagcc
    11041 aaagaaagca ttttagacta tagctttgct catggatatt gtgaaattgc atatgcttta
    11101 tttgcctatt ctaaagtctt agaaccttct atgttctata atgatctcca tacattccat
    11161 gctgaattaa aaaaattatt agaaaaagtt acttctaata ctgaaaattt aggaaattta
    11221 caactttctt ggtgcgaagg aatttccgga ataatcttat atctttgtat gtacgattgt
    11281 gacggaaaca aagatattat tagtaaatat caagaatttg tttttaacca tcatctaaaa
    11341 atgatgacag gatattgcca cggaataact agcttactac aaaccactgt ctacaatcaa
    11401 aacaaattac tgatgaaaaa aatccaacag gtaattttag catgttctga acgagatgat
    11461 cacggtttac tgatgtttca aggagatagt ggtaaagcag atttgtttga cttcggaata
    11521 ggaagcaggg ggtatattgg tgtctattaa ataataaatt cccattcgat gtgcaaacat
    11581 aaggagaaat gtatgaaatt attttggaca aacaacatat atagacagtt gctgctaaac
    11641 agctgttttt catcattcgg cgacagtatt ttctacctcg ccattatcaa ttatgtggct
    11701 cagtacaatt tcgctccgct agcgatttta ctgatttcca tttcagagat ggttccccta
    11761 ctatcgcaac tctttctcgg gattctagga gattttcaag aaaatagagt caaacacgca
    11821 ctctgtattg ccaaaatcaa aatcctgctc tacgctattt tgacagtatt tctcgtcttg
    11881 tcgccctttt cattagtttc agtcattatg attgtcatca tcaacctcat ctctgacacc
    11941 ttgagctacc tgtctgccta catgatgaac gccctctaca tcagtgtaat taaggatgac
    12001 ctgcatgatg ccatggggtt caggcagtct ctgatgaggg ttgtccacat tgtcgccaat
    12061 ctggctggcg cattccttat caatgttata agtattcaaa ctatttccct tatcaacact
    12121 ctgacttttg tcattgcctt tttgggcctg tatgttattc gacatacctt gtatgaggtt
    12181 gaaaaaagga ttgaaatgtc acatacagca ctgagtttta agaaatattt tcaacatctt
    12241 aaacagtcgc tggctgtgct cctgaggtta aaagataccg tcatactact gtttctgacg
    12301 accagtatga ttgccatctt ggatgtgtcc cctcggctga ttgccctccg cttcatccaa
    12361 cagacactag cacaactgag cattgggcaa ctcttcgccc tgctctccat catcatgtct
    12421 tgtggagcta tccttggcaa tatgaccagc agtaatctat ttaaaaatat ccgtttcacg
    12481 cacctcttgg ttttctgtga gatttcccta ttggctctaa taactagtat cctttgtcaa
    12541 gcctatatcg taattttcgt gaccagtttc atcagttcta cgattatcgg cattctcagc
    12601 cctcgcctac aagcagctgt ctttgcccat atccccagtg acaagatggg gacggtgggc
    12661 tctgctctga gcacagtaga cattctcgcc ccgtccctgc tctccctatt agccctatcc
    12721 atagcatcgg gcgtttcggt gcagttagca ttgatatttt tgtatcttat tttgattgct
    12781 cttatctttt gtcaatggtt agtcaagttc aacactcata actaacgaaa aagcatgtgt
    12841 agatttcaca tgcttttaat ctccccaatc gtcaggtcaa gtacaacaaa gtcacttctt
    12901 tgattaagcg agtgttctaa tataattata agcgccctgt cattaccgaa cccattcgcc
    12961 attatagttg acagaatagc catctacggt cgtattcact gccaaagcac ctgagctata
    13021 agcatagtac cagttgccat tgacctggaa ccaacctgtc ttcatgtctc cattacctgc
    13081 atttaggtag taccaagttg aaccatcttg ataccaacca gttgccatag ctcctgatga
    13141 acggagatag taccatttgt tcccaaggtt ttgccaacct gttttcatat cgccatttgg
    13201 ctggtctaaa taataccaag tggtaccttc ctgataccag ccagtggcca ttgctcctga
    13261 ggaacggagg tagtaccatg gagcaggttt gcttctatca atgtcaatag taacatgaaa
    13321 atcttgagtc caacccttag caacttcacc aattggtagt ggattgtaag aatcatcacc
    13381 aaaatttcct agcttattta aataatacca attgccgtct tcataaatcc agcctgtctt
    13441 tacagcataa ttattatcaa aataataagt tctcttttca gcagatagag gatattgttc
    13501 accgtatact tttcctgtat tttcagacgt ttttgcctct agtacttgtt tatctgtttg
    13561 ttctagcaaa gcaccatctt gtccaaaata gtaccacttc tcctgaaggc caatttcgaa
    13621 gactggacga ttatcaaata aatcatcacg ataacctgtt ccaggaattt ctaagtattg
    13681 ccaaccgaca accatctctc caatatcacc aaaatagtaa gttttaccgt ctattttatg
    13741 ccaatagatt gctttatgat catcttttac ataatatttt ctattatcct tatcaacaaa
    13801 tcggccacct gtggtattcg caaataccgt atttgtagct agcaaaccaa agaaaaagac
    13861 tgctagtaga acttgcatca tttttttcaa aagtttcatt tatatatcct cctaaaacga
    13921 ttgtagcaaa gacacgactg catgtcaata tataaaaact cctcaaaaaa gcagttacac
    13981 aactgcaact gcttttctat tcttgcatgg tgtaaccaac accacgcacg gttttaatgt
    14041 agcttttttg tcctttaaca tcaagcttgc tccgtagata gcggatatag acatctacga
    14101 tatttgtctc ggtcgcactt tcatacttcc aaacactttc caacaattgc tcacgagtca
    14161 atactttctt gcttcccata agtgtcgcca aaaggtcata ctcacggcgg gtcagagcaa
    14221 tcatctcttc accacgataa accgtgtgat gttcaacatc tatcctaaga ttgcggtagg
    14281 tccttggaac tttcatcaga ctgcagtgtt gatcaatgaa gtcccgacct cggaagatcg
    14341 ccgaaatacg cgctaccaga ttttcgataa agactggctt atagatgtat gaaactgcaa
    14401 aacgctgaac aacttccagc tcttcttgca agtcttccca atgatctaaa atcatgatga
    14461 ctgaggcagg tttagttcga ctcaattttt ctgcaaaatc ctgagccatc atatctccca
    14521 gattaacgtt caataaaatc aaatcatagt ctgtctgaag agccatggaa agggcttttt
    14581 gcccctcctc taccagatca acccgatact gctctttctg gagttccaaa cttaaaaaat
    14641 gagctagatt tcgttctttc tcaagtaata aaatccgttt ccccatggct gacctactta
    14701 tttttcgtca taccaagagt agtggaaggt tccttctttg tctttacgtt ggtaagtgtg
    14761 agcaccaaag tagtcacgtt gtgcttggat caagttagcc ggaaggtcag ctgaacggta
    14821 gctatcaaag taagtaatag ctgctgagaa agttggcact ggcacaccag cttgaaccgc
    14881 aagagctacg atatcacgca ctgcttgttg atacttagca gtaacatcca agaagtactc
    14941 gtccaaaaga aggttggcaa gatctgcatc gcggttgtaa gcatctgtaa tcttttgcaa
    15001 gaaacgagaa cggatgatac agccatcacg ccagatagat gcgatatctg caaatggcaa
    15061 gttccagttg ttttctttag aggctacacg caattgagca aatccttgtg cgtatgaaat
    15121 gatttttgag aagtaaaggg cttgacggat tttttcaatc aattcagcct tgtctccttc
    15181 aaagttgaag gcagctggtt ttggaagcac cttgctagca tgtacacgtt cttctttgta
    15241 agttgaaatg tagcgtgcaa acactgactc agtaatcagt gacaatggta caccaaggtc
    15301 aagagatgat tggctcgtcc atttaccagt tcccttgtta cctgcagcat caaggatgta
    15361 gtctacgatt ggtccatctt ggtcttcatc gtctttacgg ctcaagatat cagctgtgat
    15421 ttcgatcaag tagctatcca attcaccctt gttccactca gtaaagattt cagccatatc
    15481 ctctgcagaa aggcctagca agtgttgcat caagtcatag ctttctgcga tcaattgcat
    15541 atcaccgtac tcgataccgt tgtgaaccat tttcacatag tgaccagctc catcaggacc
    15601 gatataagtc acacatggtt tgccatcttc tggtgcttta gctgagattt cttcaagaac
    15661 atccgaaacc aattcgtagg cctctttttg tccaccaggc atgatagaag gaccttcaag
    15721 ggcacctttt tcaccaccag aaactccagt accgataaag ttgatacctg agtttgccaa
    15781 ttcttcatta cgacggatgg tatctttgta gaaagtgttt cctccgtcaa tcaagatatc
    15841 acctttgtca aggtgtggaa gaagggcttg gatagtagca tctgtaccag gtccagcttg
    15901 aaccatcagc atgatacgac gaggtttttc gattgagttt acaaaacttt caacgtcata
    15961 gcttggtaca aaattctttt caggatggca agcaatcaca tcttccgttt tttctttact
    16021 acggttgtag atagcaactg tgtaaccacg tgattcaata ttaagggcaa ggttacgacc
    16081 cattacggcc atacctacga caccaaagtt agcttttgtc atttgacact cctcttgttt
    16141 catttgtttt attttatcat ttttactttt gaaaagaaag aattttgtaa cgactgctta
    16201 gtagtccaag tcatccgcat gaccagcgcc atttccgaca aagtagcctg tcttacagtt
    16261 aagggtaaag agataggttc catctggctt atagaggttg taataaccat tgccgttaac
    16321 gatattgaca cgttcaagga tatattggtc tccagtgata tagccacgtg aacgtgaagt
    16381 tgctagaatt tgttccaaaa caccatccgc aaaatcccag gcagagttat tactatcatc
    16441 aatagcagac tgattatagg caacacgact ggcacttctt tgaacagcaa cccctgcact
    16501 cgagagaccc atattgactt cactgcgact acttctagtc tcatttgaac tacttggttt
    16561 tggctcacta gtcgtatttg aacttgcttg acttgaactt gagctgctag tttgacttga
    16621 acttgagcta gaagtacttg tttgctggct cttaccaaga ctgatagcct tatctagcac
    16681 tttatcaagc tccgtatttc cagttttaat atccgtaaat ttagcatccg atttggcttt
    16741 ggcattggta tccaacacac catccacaat agctggtttt tcaaattgtg cattgacatc
    16801 ttgaatggcc ttgatttgcg ttactagact atcatattta gatttggcaa gcgtatgttc
    16861 acgactacct tccagcttat caagtaaagt cttgagttga ctcagtttat caaactggct
    16921 atttttcaaa gccgttttat tgctatttgt gtaaaaggcg tcataaagtg tattaaaatc
    16981 atccacatcc gattgattgg ctgtagttga ttgagaagtt tcaatttcct tggtcgaacg
    17041 agccacttga cgatagacat aataagcact gacacaaata attactgata caagcgccaa
    17101 aacggtcaaa ataaagcctt ttttactttt cttttctgag tcctcttgat ccagacgaga
    17161 aaggttttgc tcctcttcca cttcttcagt catttctgtt ggattttcat catctagcaa
    17221 tagaggatcc aagccttgct cctcatcgtc cagaggcaag ggtagtaaga tgtcttcaga
    17281 agatggaact tcctccgaag aagcttctga cttctctaca gcctcacgca tctcttgaat
    17341 taaatcatcc agactctgag tttcaacgaa ctcctctttt ttgtattgac gagtcgcaaa
    17401 cttatccgcc tcaatttcat ctctgtgttg cttaacatac ttgtccaaaa tggaatcctc
    17461 aggcaagact cctgcttcca cttcttcatt tttacgaata gcttgaccaa ctgttagctc
    17521 ttttgcttca tcaaaatcaa attgcggttc ttgaccttct tttttatgac gatttcgtct
    17581 ttttttactc atgagtatcc ctttctattt tacctcttgc cgtttgacac gctgaccaaa
    17641 atattgatat agatccactt tcaaggttcc gttgtataac ttacgcttct tatctgcttg
    17701 gctaccatac ttgctttcaa aagcttcatc actagtcagg ataaatttgc tccaagtttt
    17761 cagcggtgca aatacttgcc ccatctcagc atagagcttg gtcactcctg catcatctga
    17821 caaacgttca ccataaggcg gattggaaat gattactcca ttgattttat cggaacgtaa
    17881 atcctgcacg cgcatctgct taaaagtaat atctcctgca acaccagctg cctgagcatt
    17941 ggccttagca atttccacca tgcgagcatc aatatcacag cccatgatat ccaactcaag
    18001 cttacgttct acttttttag ccgcttctgt gcgcacttct tgaatcaagc gatcgctgat
    18061 ccagttccat tcctcaaatg caaaagagcg acgaagacct ggcgccatct ttctagcaat
    18121 cataactgcc tcaatacaga aagtccccga accacaggtc ggatcaatca aaggcttgtc
    18181 tggataccag ttagaaagtt gtaaaatggc tgctgccata ttttccttga taggagcgcc
    18241 acctttttcg gtacgataac cacgtttaaa gaggctagac ccggtcgtat caatcatgac
    18301 agttgccaca tctttgagaa tagagacctc aatcttaaac tctgggccat tctccatcag
    18361 aggaacccct tctgggcgag catagtgttt ctgcaatttc ttgacaacag ctttcttaga
    18421 aatagcctga acactgggct cattgtgaag tttggactta acacatttag cttttgaaat
    18481 cggtaaccga gctccaagtg gtaaataatt ttcccaatct aaagcgaaaa ctccctgaaa
    18541 tagctcttca aaagtcttag ctgggaacgt tcctacgata attttgatac gatctgctgc
    18601 ccgaagccaa aggttggttt cgataatagc tctcacgtct ccttgaaaac gaacacgtcc
    18661 attttcaacc tgacaatcgt agcccaactc tcgcacttca cgaccaacga cagcctcaag
    18721 ccctgctgcc acagttgcaa ttaaattaaa ttcttttttc atgttacagt cttgcaagac
    18781 ctttctatat gtccacttta aaaaatgagg ttgagaaaat ttctcagccc caacctatat
    18841 gcaattttct ataagccatg ttttgttcca gaagtgacta ggaccacttc cttcgataat
    18901 catctgtcta ccactaacag ctctagctga tagcagtcag aatactacgt tcgtttccgc
    18961 atactccatg ccccgaccaa aatttgggtt gctagcttga ggggtttacc gcgttccact
    19021 ctctctgttt ccagaaagac tccgtcactg tggcactttc aagcctactc tggcctatcc
    19081 aaggacttag ccatttcaac tgccgtaacg atttctcgtc cctaggctta tggtttcgcc
    19141 tagcacaaac actacaggca tcacagcctg tgctagcatg gactttcctc atgagaagca
    19201 attctcctca cgcgattatc caaaaattgc acatctcaaa taactactta aaaatctgag
    19261 ttatctaaaa tttgtttacc aaaaacttct ttttccaatc tattcaggcg tttcaaaata
    19321 tcaaaattcg tcatagaact tgtaattgcc gcttcaaggg gttctgcttg aactggtgaa
    19381 ggtttcggtt tacgagttaa ttcttccttc aaatccgcaa tttcctgacg aagtgacttg
    19441 accaaggcag cataggtttc atagtccttg atgacatcgt ctaaaaactc gtcaacttct
    19501 actttattat agccacggac ttcacgtcca aactcttgtt caaaaatatc tttcgctgaa
    19561 aaaataatac ttgccatgtc tctctccatt ctcgcttgct agtattatta tataaaaaaa
    19621 ggcaaacaaa aatcaaggtc aaagcttcaa ttttcggaaa aattttcagc aagttcattt
    19681 aattgatcaa atgttaatct ctttataaaa tagtcctctt gatttttcat cttttggtaa
    19741 aaataagcta gttttgtttc attttcttca tcataaaaaa gataggaact agtcgtgttt
    19801 tccagcaaaa actgctggta atctcttaac tgccccttgt gttcatagcg aggataagca
    19861 tatttgacaa agtctacctg cttaaagcga ctcagtttca tctgattgcc ttcattccaa
    19921 ttttccccat gggtttcaaa agcaaaaatg gtggccaact ggaaaccgta ttcggttttc
    19981 atctcctgag caacctctaa aacccaatat tcaaaaccca aactccctgt aaacacaagc
    20041 caagacaccc catctgctgc catagcctct aaatctttac gtatcgcttt tttaatcaat
    20101 ttaagacgag gatccttatc agaaaataaa cccaaatcaa aagcagaata gcctaaaacc
    20161 aaagctgtag ccatttttaa ccctttctgt gcaaattatg gtataataaa gtgatgttat
    20221 tgtaccaata aggagaatta tgatcaacta tccacataaa gtttcatcac aaaaaagaca
    20281 aacatctctt tctcaaccca aaaatttcgc aaatcgagga atgtcttttg aaaagatgat
    20341 caatgctacc aacgactact atttgtctca gggcttgact gttatacata agaaaccaac
    20401 tcctattcaa atcgtacaag tggactatcc acaacgaagt cgtgccaaga ttgttgaagc
    20461 ctattttcga caagcttcaa cgacggacta ttctggcgtt tataatggat attacatcga
    20521 ctttgaagtc aaggaaacaa aacaaaaacg tgcgattccg atgaaaaatt ttcatccaca
    20581 tcagattcag catatggaac aagtccttgc ccaacaagga atctgctttg tccttcttca
    20641 cttttcttct cagcaagaaa cctacttatt gccggcattc gatttgattc gcttctatca
    20701 tcaagataag ggacaaaaat caatgccact tgaatatatt cgagaatatg gatatgaaat
    20761 caaggctggt gccttccctc aaattcctta tctcaatgtt atcaaagaac atttattagg
    20821 tggtaaaaca agatgaacaa accaacgatt ctgcgcctaa tcaagtatct gagcattagc
    20881 ttcttaagct tggttatcgc agccattgtc ttaggcggag gagttttttt ctactacgtt
    20941 agcaaggctc ctagcctatc cgagagtaaa ctagttgcaa caacttctag taaaatctac
    21001 gacaataaaa atcaactcat tgctgacttg ggttctgaac gccgcgtcaa tgcccaagct
    21061 aatgatattc ccacagattt ggttaaagca atcgtttcta tcgaagacca tcgcttcttc
    21121 gaccacaggg ggattgatac catccgtatc ctgggagctt tcttgcgcaa tctgcaaagc
    21181 aattccctcc aaggtggatc aactctcact caacagttga ttaagttgac ttacttttca
    21241 acttcgactt ccgaccagac tatttctcgt aaggctcagg aagcttggtt agcgattcag
    21301 ttagaacaaa aagcaaccaa gcaagaaatc ttgacctact atataaataa ggtctacatg
    21361 tctaatggga actatggaat gcagacagca gctcaaaact actatggtaa agacctcaat
    21421 aatttaagtt tacctcagtt agccttgctg gctggaatgc ctcaggcacc aaaccaatat
    21481 gacccctatt cacatccaga agcagcccaa gaccgccgaa acttggtctt atctgaaatg
    21541 aaaaatcaag gctacatctc tgctgaacag tatgagaaag cagtcaatac accaattact
    21601 gatggactac aaagtctcaa atcagcaagt aattaccctg cttacatgga taattacctc
    21661 aaggaagtca tcaatcaagt tgaagaagaa acaggctata acctgctcac aactgggatg
    21721 gatgtctaca caaatgtaga ccaagaagct caaaaacatc tgtgggatat ttacaataca
    21781 gacgaatacg ttgcctatcc agacgatgaa ttgcaagtcg cttctaccat tgttgatgtt
    21841 tctaacggta aagtcattgc ccagctagga gcacgccatc agtcaagtaa tgtttccttc
    21901 ggaattaacc aagcagtaga aacaaaccgc gactggggat caactatgaa accgatcaca
    21961 gactatgctc ctgccttgga gtacggtatc tacgattcaa ctgctactat cgttcacgat
    22021 gagccctata actaccctgg gacaaatacc cctgtttata actgggatag gggctacttt
    22081 ggcaacatca ccttgcaata cgccctgcaa caatcgcgaa acgtcccagc cgtggaaact
    22141 ctaaacaagg tcggactcaa ccgcgccaag actttcctaa atggtctcgg aatcgactac
    22201 ccaagtattc actactcaaa tgccatttca agtaacacaa ccgaatcaga caaaaaatat
    22261 ggagcaagta gtgaaaagat ggctgctgct tacgctgcct ttgcaaatgg tggaacttac
    22321 tataaaccaa tgtatatcca taaagtcgtc tttagtgatg ggagtgaaaa agagttctct
    22381 aatgtcggaa ctcgtgccat gaaggaaacg acagcctata tgatgaccga catgatgaaa
    22441 acagtcttga cttatggaac tggacgaaat gcctatcttg cttggctccc tcaggctggt
    22501 aaaacaggaa cctctaacta tacagacgag gaaattgaaa accacatcaa gacctctcaa
    22561 tttgtagcac ctgatgaact atttgctggc tatacgcgta aatattcaat ggctgtatgg
    22621 acaggctatt ctaaccgtct gacaccactt gtaggcaatg gccttacggt cgctgccaaa
    22681 gtttaccgct ctatgatgac ctacctgtct gaaggaagca atccagagga ttggaatata
    22741 ccagaggggc tctacagaaa tggagaattc gtatttaaaa atggtgctcg ttctacgtgg
    22801 agctcacctg ctccacaaca acccccatca actgaaagtt caagctcatc atcagatagt
    22861 tcaacttcac agtctagctc aaccactcca agcacaaata atagtacgac taccaatcct
    22921 aacaataata cgcaacaatc aaatacaacc cctgatcaac aaaatcagaa tcctcaacca
    22981 gcacaaccat aacatttatc acccagattt ttctgggtga ttttgtcata gcactattgt
    23041 atttatgtgc ttaacaaaag cctgagaaga tgtcttctca ggctttttct gtactataaa
    23101 cctcattgga aaagatagag actgcttccg taatcttgtt aagaggtttt actaaatact
    23161 agtctttctt cgtttttact acaaagagag cagatagggc tagactaaca cttgctagga
    23221 agagggctgt gtcagattga ctctcacctg ttgctggcaa gatttgttca ggtgtttctg
    23281 gtctagctat ttcttgctta taatctggcg tcttaccttg atcggaaact aaagagctaa
    23341 gtttaaactc aggtttctct acagttggag catctggttt cgtagcagaa tcttttaatt
    23401 ggttgagata atctgcaaag tattccacca ttctttccgt caagagatgg ttatccgtcg
    23461 catatttcat gcttgcctga acgctagcta cttcctcttg attgtctttg tctgttaatt
    23521 tctgagcttt agccaaagct gcttcatagc ctgacaagtc aagaggtgtt tctgctacct
    23581 gtggacgagt aaagatgagt tccgctgcag attggtattt atcgccacca tctccgtaag
    23641 tcttagtacc tgtaaggaca attttcttag ccttaattgt cttaccaaaa tcaatgtctt
    23701 ttggcttatt gttatctggc caatcagttg cagtaaaggt atgctccttg ccagactcat
    23761 ctgtcacaac aagtttcaca tcacgcaagt taccatttga acctgatcca cgtggaacgt
    23821 aacgaagtcc agtgatttca gttggttctt tcaagaccat ggttgcaggc ttgcctacat
    23881 ctccaccatt ccaagatgta tgccataaac tagataagtt tccatcaaag gcatttgcaa
    23941 gaccttcttg agcctgagca ggagctgtaa gacttgcaaa gtcatctgct accaaagccg
    24001 ttttcttctg aaccaaagca ttcttcaagg cttcaatctt ggcaatctct gcacgcgctt
    24061 cttccacact gatatcatca tcggcctgac taaggttaaa gaccgcctct ttcaaagcat
    24121 ccatagactc tttggtgtag ttagtcatgg caaccgttgg caagtagttc ttcagagcat
    24181 tttctgtcaa catcttacct gttagggtaa tttcttcgat ttgaagatta tccatcatga
    24241 agtcgttata accacggaag ttggcatttc caccagaatc accacgagta ttacttgcat
    24301 tgccagttga gtagatacct acccaagtat cccctgtttc tgcacctgtc acaaggaagg
    24361 ttgccttctt ggctttctta gaatctgtcc aagtatttgg caattcatgc atttccaagt
    24421 tgcttgcttg agtgccacga cgacctgact ggaattctcc cttaccgact acaaaagcat
    24481 aggtattgtc tgaacctgct tcgtattcaa aggttacacg gtaggtctta cctgcttcaa
    24541 aacggaagtt ttgtgggatg gtttggtaaa ccaagttacg acggctcact agtccatttg
    24601 tcttgagtga ccaatttcct tcgataacat catcgacttt cttaccgttc caaccacgtt
    24661 gtgtatatgg atcgtgtttt tcagacaagt gagtgcggtt gtcttcgaca ccttcgacac
    24721 cacccactac aaatgggaag attccttgag ctacattttc aaagtcttgc ttgaaggttc
    24781 cttgacctgt atcgtgcttg tctccataca tgcttgaatt gttttcaaag gtacgaattt
    24841 catcaaagta agttgcttga tcaccagctt cacgactcaa tgtcagagta acatttgaga
    24901 cgtccgatcc tgttgtaaag aaggcgtaca tgttttggaa gtaacttgta tcgttaactg
    24961 tagcattgtt acgacgtgta ttgtgggcgt aggcttttac atagttgagg gcgagagact
    25021 tattggtata agtagtcact tctttttcac cagtattcac agtgatactt gccttggcat
    25081 tactacggtt atcgacaccg acataaacgg catacttggt atttggtttc aagccagtta
    25141 atttctgagt gagactaact ttttctttgt ttccttgaat acgaagcata tcgtttgccc
    25201 cttgagactt gacaatttct gccttagaag catcgcctga aatggtccaa tgtttcaagg
    25261 taccactgtt aaatccttgg tcatagatgt gcatgccttc actccatgac atttcaggat
    25321 tggtttgttt tgaacgatag agaacgtatg gttgatttgc tagaagatct agggtaattt
    25381 taccatcttt tacagttagt tcttgctctt ctgtcttacc ttggtcagtt agcttgtaaa
    25441 ggtaaacctt gctctttgcc caatcgcttg gaagggtcca agttgttgca ccggcctgcg
    25501 tattgaagta gtacatcttt tccttatcag tagaaagttt cttaccattt gcatcccagt
    25561 tccaaggagt caagtaagct gaaccatctt ggatgacacg tccgttgaga gttactgtac
    25621 gttcgcgata ttgtggacta ttgacatcat ttgacttacg agttacaact actttattat
    25681 tgtcagcatc taccaattcc actcgcattt ctggagtcca tttataggta ctaccgttat
    25741 cggtcatagt caccggtgta ccattttccc atttacttac agtgaagtgt tggaagtact
    25801 tagtcatgac gtcatgggca aataagttgg ttacatagcc attgtagtca cttcttccct
    25861 gccagccttc aaagtctttc atgctgtagc cacctagcag tggatagttg gctgcaccac
    25921 cataacttct gtagtcccca acccaagcat ctttttggtg gttacggata aagcgggtga
    25981 tggcactgtt gataccttta ttggtgtagc caccgtaggt caagtcagct gcccagtgat
    26041 ggaaggtaga gtcgtactca ccaccatggc cccactcgat cgcaaagcgc cagccttgtt
    26101 tgttaatttc tttagcaaga acgtgggtag cccaggcacc gttatcacct gattgaccat
    26161 taccccaaac gtccacatag ataaagtcga gaccgtcacc aagttttttc ttcaaatctt
    26221 cccaacgtgc caaacgacca tgagctaggt cataggcagc atcaatgttg ataccttgat
    26281 ctagccagtt ccaaccatag ctatagcttc catctggatt cttacggaga attttttcat
    26341 tgaagtattt agactcagga taagtttctg aagcgttaac gtggatacct agatgagctc
    26401 catatttctt agccttctca attagggtct tgaagtcttc gacaccaccg atacgcttac
    26461 caatatcagc atagttcaag tgaccagagt catggccttc gctaccatat cctttaagga
    26521 gaacaccttg cccaagacca tctgtgtgga gattgatttt cttgatacca tccaaggtca
    26581 taaggaatgg attttgtgct tgagaaccaa agttcatcgc gatacggtaa gctgtgatat
    26641 ccttaacttt ttcccaacct tgaggattgt tcataatgct acgataagca atggcaccat
    26701 cttgccaatc gactttcttg tctgcattgg catcttcagt gataacaacc ttagcacttg
    26761 gaagttcctt cgtgtattct gggaaaacaa tgcccttata agctttttcc cattgccatt
    26821 cagagctgtg gattcctaca tagttggcat ttccgactgt ttctttataa gctgtcaaac
    26881 gagtccagtc attcgaacca ccaccatagc tattttgaga gttactccaa acaccagcag
    26941 caagcttatc tgtagaaaca aatccataca tgtaaccctt ggctaggtct ttcattggat
    27001 tggttacatc gatatgatca tctccgctga catgcgtatt gtttgacatg gttgccccat
    27061 caaacttagc accagtttga tcactagaaa cagagactaa agcattgccg aggaaactaa
    27121 tagaagaaag tagttttctt tcgtcatcaa tcttttgacc tggagtgact tgattgtggt
    27181 tgacaatctt ggtcacatca aagtgcaatt gattgtccac aacttgcaag cgtactgtca
    27241 tttccgcatt gattaagtga gcatcatcgc gaagcttcat caagtactct gctgttgtct
    27301 cattgatttt cttataagtg acttcagggg tgattcggtg gttattgata aagacttggt
    27361 tgaactgttg cacctgtcct ggcaaagtat gtccattcaa gctgtattcc ttgacacgag
    27421 ggaaggcttg gtcaatcact gctttgagga ccttagactg gatcgtatca taagtcacct
    27481 tgctatcgtc aacaaccgga cctgtttctt tctgggcagg agtatcatcc gcttttacgc
    27541 cttcttgatt gtctgtttta acgctgacaa ctgtacggtc attgccataa gttcctgcct
    27601 tgagaaggat cttcttctca tttttaagat tatcattgac cgccccaggc agtgtcactg
    27661 tgtcaaagag attgacatcg ttattgctgg cattgagttg tccatctgac ttgagagtga
    27721 tagaaagacg gtttacagaa cccggttctg gtgcaactac acggttgcct ttataccatg
    27781 tgctattacc tggagttttg tattcccaga accagccatc cttgtcataa ccgacaaaaa
    27841 cattattatt ggtatcttta aatttcagga agacaccaaa gcgtgatttg cccttttcag
    27901 aatcttcttt gaaggttaaa tcaacagttg catttccatt ggcatcaacg gtcaagccct
    27961 tcttttcaaa cagggctggt ttgcctgcgt tatcattttg agcagttgag aatagttggt
    28021 tgtagcggac acctttttct tctcggatag tgactgttcc ctgttgttct tttttctcta
    28081 ccgtttgcca ttcaggagtt accgtcttag gtgtttcagg tttagcagct gcaggtttat
    28141 cctcttttgg tttttcttca gccggttttt ctttttcaag tgctaatagt tcttcttctg
    28201 tcttaggtcc atctgtaact tctggagaac cttggtcttc tcccacctta ggcgcttcaa
    28261 aatcacgccc atcattctcg tttgctgttg caagagcagt agctaaatca gctggtaagt
    28321 tcgccgtgga accagactgc acgctatctg caagaaccgg acttgtccca aagaatgcag
    28381 ctccaatcat aacagaagca acacctaatg aaaatttccg aatactatat ttacaacgtt
    28441 tttcaaataa tcctttattc ataaacttct aaatcctttc attttttagc aagcgtttac
    28501 atttcctttt taaaaaatac tccttcctct gaaagtgcaa tgcttaaccc tgataaataa
    28561 aaaataattg tacttttaat ataacaaata atagctgaga aagcaatagg aaacaagtaa
    28621 aattaacaaa aattttctct attctgacaa aaagaggctc caacttttgg gggtcagtac
    28681 atttagaatc tttctttaca aatgtaaaaa aggattcagc attcaagaga aatactaaat
    28741 cctttcttat attttgcaac agttatttca catgttttgc gagatcttct tgagcctttt
    28801 tattagactc ttctttttct ttcatccatt tttcctgagc tttttggtat tcatctacag
    28861 tgactgcctt gtcttgaagt tccaagtatt tatacaagac tggttcactt gtacctttat
    28921 ttcctgacaa tgcaaatggt attgtaaatg gtaccatctt agacaagatt gggcgccctg
    28981 tacgagatgt agttggaata atcaaagcac tatctgtcaa ccaagcttgg gctgcagcgt
    29041 atttatcata gcgtttagca acatctgtag cctcatcacc agcctcagtg accaattttt
    29101 cgtagtcata tagacctact tttttagcag ctacattatc ttcccctgag tcaaacccta
    29161 aatatgtttt agtactttct cctacagaag gtttgataat atcaaggtag gttgatggat
    29221 cggcaaagtc tggaccccaa ccgacattat ctgataaatc ccagtcttcg ccagcagcat
    29281 tttcagcaaa atatgtaata ttgtttactt cgtctttttg tagttgttgg atatcaataa
    29341 tgacattatc agctcctaaa gttgcttcca aggattgttt catagattgg acgcgctgaa
    29401 cttttgtagt tgctgtctgg tcaactggca tatccaaatg aattgggaaa gtcacacctt
    29461 ctgcttgtaa ggctgattta gctttagcaa attcagcctt ggctttttct ggattgtaaa
    29521 gaccatcctg agaatctgca agattaacat ccttccattc atccccataa gtgaccaatt
    29581 tctctttgac catatcgcca aagtttttac catctgcttg aacaaatgtt ggtggaacaa
    29641 agatattacg taagattttg cttgctccag tttgtccatt caactgagag gcataggctg
    29701 tacgatcaaa accaaaggca atagcctgac ggaaatcctt gtttaagaga gcctttttag
    29761 tcgatgcctt ttgttcgtcg ctggtcttag atgtatattt ataggactga cggtcaatat
    29821 ttgtaccaac tagatacgta atagagcctt gttgagtata gacaatattg tccttcatac
    29881 tcttctcaag ctctgcgaaa cttgcacttg ttggatagag acgagctgct gtaaggctac
    29941 catctttaaa gttttctgca ggtttgctgg tatcttgacc atcccagaat gacaatttaa
    30001 ctttgtcaac atgcacattg tccttatccc agtagttcgg atttttcgca aattcaacag
    30061 aggattttgt cacaatggat ttcaacaaat aaggaccgtt atacaagaga ctacttggat
    30121 ccgtcgcttt ggcaaaatca tccccttttg agttcaaaaa ctcttcatta actggcgcaa
    30181 gcacacccat ggttgtctta gaattccaga agctttctgg tttgttcaaa gtgtactgaa
    30241 ctgtctgatc atccaaagcc ttaattccta cttgtgagaa atctttgatt tcccctttta
    30301 cataggcatc caaccctttg attgattctt gaacaaggta aagagcatct gattttttat
    30361 cagcagcata ttttagtcct gttacaaagt cttgagcttt gactgccgcg tattcttcac
    30421 cttcagaagt ataccatttt gcatccttac ggatagtata agtgtaagtc aatccatcct
    30481 tggatacaga ccaatcctca gccatagacg gcacaaagtt cccgtagcga tcattttcta
    30541 gcaaaccatc aaccacgtta ctggtaatat ttgctgtcgc agccttagca gttgtcaaat
    30601 agttgaggtt atcagggtct gtctcataaa tgtatgagaa tgtcttctca cctttagtgc
    30661 ttgaacctga tccagagcat gcagctaaag tagtcgccgc caataatgtc acgcccgcaa
    30721 gggcaagtag ttttgaactt ttcatccttt tctcctttaa aactttctct ccattataga
    30781 ctcttttcag aaagttgtca acagaatttt cagaattttt gaaaattatt tttcaaataa
    30841 catctttgca aaaaatatga atatcgtaac cgcccaataa cgaagtctat tgaaaaatct
    30901 ccagactaga gaactca
//
LOCUS       DAVBCG010000013        28492 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBCG010000013 DAVBCG010000000
VERSION     DAVBCG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.013"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            49..537
                     /locus_tag="VQ495_000480"
     CDS             49..537
                     /locus_tag="VQ495_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2530477.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            534..1721
                     /locus_tag="VQ495_000481"
     CDS             534..1721
                     /locus_tag="VQ495_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2530478.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            1714..2079
                     /locus_tag="VQ495_000482"
     CDS             1714..2079
                     /locus_tag="VQ495_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2530479.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            2095..3441
                     /gene="asnS"
                     /locus_tag="VQ495_000483"
     CDS             2095..3441
                     /gene="asnS"
                     /locus_tag="VQ495_000483"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2530480.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            3594..3884
                     /locus_tag="VQ495_000484"
     CDS             3594..3884
                     /locus_tag="VQ495_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2530481.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            3896..4366
                     /gene="ssbA"
                     /locus_tag="VQ495_000485"
     CDS             3896..4366
                     /gene="ssbA"
                     /locus_tag="VQ495_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2530482.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            4398..4637
                     /locus_tag="VQ495_000486"
     CDS             4398..4637
                     /locus_tag="VQ495_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2530483.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            4932..6332
                     /locus_tag="VQ495_000487"
     CDS             4932..6332
                     /locus_tag="VQ495_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2530484.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            6334..6693
                     /locus_tag="VQ495_000488"
     CDS             6334..6693
                     /locus_tag="VQ495_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2530485.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            6747..7496
                     /locus_tag="VQ495_000489"
     CDS             6747..7496
                     /locus_tag="VQ495_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2530486.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            7486..7785
                     /locus_tag="VQ495_000490"
     CDS             7486..7785
                     /locus_tag="VQ495_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2530487.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            7856..8791
                     /locus_tag="VQ495_000491"
     CDS             7856..8791
                     /locus_tag="VQ495_000491"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2530488.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            8878..9390
                     /locus_tag="VQ495_000492"
     CDS             8878..9390
                     /locus_tag="VQ495_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2530489.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGAVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            9427..10022
                     /locus_tag="VQ495_000493"
                     /pseudo
     CDS             9427..10022
                     /locus_tag="VQ495_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            10123..10317
                     /locus_tag="VQ495_000494"
     CDS             10123..10317
                     /locus_tag="VQ495_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2530490.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(10363..11808)
                     /locus_tag="VQ495_000495"
     CDS             complement(10363..11808)
                     /locus_tag="VQ495_000495"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2530491.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            11914..13536
                     /locus_tag="VQ495_000496"
     CDS             11914..13536
                     /locus_tag="VQ495_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2530492.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            13740..15698
                     /locus_tag="VQ495_000497"
     CDS             13740..15698
                     /locus_tag="VQ495_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2530493.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFATAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            15870..16805
                     /locus_tag="VQ495_000498"
     CDS             15870..16805
                     /locus_tag="VQ495_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530494.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFE"
     gene            16830..17482
                     /locus_tag="VQ495_000499"
                     /pseudo
     CDS             16830..17482
                     /locus_tag="VQ495_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            17640..18734
                     /locus_tag="VQ495_000500"
     CDS             17640..18734
                     /locus_tag="VQ495_000500"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2530495.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            18742..19908
                     /locus_tag="VQ495_000501"
     CDS             18742..19908
                     /locus_tag="VQ495_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2530496.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            20001..23099
                     /locus_tag="VQ495_000502"
     CDS             20001..23099
                     /locus_tag="VQ495_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2530497.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            23147..23764
                     /locus_tag="VQ495_000503"
     CDS             23147..23764
                     /locus_tag="VQ495_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2530498.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            23774..25108
                     /locus_tag="VQ495_000504"
     CDS             23774..25108
                     /locus_tag="VQ495_000504"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2530499.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            25118..25582
                     /locus_tag="VQ495_000505"
     CDS             25118..25582
                     /locus_tag="VQ495_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530500.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            25579..26073
                     /locus_tag="VQ495_000506"
     CDS             25579..26073
                     /locus_tag="VQ495_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530501.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            26154..27803
                     /gene="mltG"
                     /locus_tag="VQ495_000507"
     CDS             26154..27803
                     /gene="mltG"
                     /locus_tag="VQ495_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2530502.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            27874..28356
                     /gene="greA"
                     /locus_tag="VQ495_000508"
     CDS             27874..28356
                     /gene="greA"
                     /locus_tag="VQ495_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2530503.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT         8539 a         5314 c         6418 g         8221 t
ORIGIN      
        1 ttcttatatt cacttgtaat aaagcttaat agatggtaaa atagacgagt gaaaaaaaga
       61 caaaacaaag caaaaaataa tctactgtgg cagtatggtc tagggatgac gattttgttt
      121 gtggttatca gtgcttcctt tctgtatatg gtttctctta gcatgaaacc ctatcaaaca
      181 gctaaaagtg aaggagaaaa attagctcag cagtatgcag gattagagca ggccgatcag
      241 gttgatttat acaatggctt ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa
      301 gaagcacttg ctgttctgat tggaaaagat gatcataaga tttacgttta tcagctaaat
      361 cagggtgttt cacaagaaaa agcagaaacg gtttctaagg aaaagggagc tggcgaaatt
      421 gacaagatta tctttggtcg ttatcaagat aagccaatct gggaagtcaa gtcaggatct
      481 gatttttatc tagtagattt tgaaacagga gcattggtca acaaggaggg cctatgaaat
      541 tatccaaccg tgttttagaa atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca
      601 aagcactgaa ggctgagggt agagatattt tgtctctaac cttgggtgag ccagatttca
      661 ctactcccaa aaatatccaa gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt
      721 tttatacagt aacctcaggt ctgccagagc ttaaggcggc ggtcaatagc tactttgagc
      781 gcttttacgg ctattctgta gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt
      841 ctctctatac cttctttatg gctgtggtca atccaggtga tgaagtcatc atcccaaccc
      901 catactgggt tagctatgga gatcaggtca agatggcaga aggcgttccc gtctttgttt
      961 ctgctaagga agacaatcac tttaaggtga ccgtagagca gttagaagca gctcgcactg
     1021 acaagaccaa ggttttggtg ctgaattcgc catctaatcc cacaggtatg atttacaccc
     1081 gtgaggaact cttggcaatt ggaaactggg ctgtagaaaa tgatattctc atcctagcag
     1141 atgatatcta tggccgcttg gtttataatg gtcatgagtt cacaccgatt tctagcctat
     1201 cggaagcgat tcgcaagcaa acagtggtca tcaatggtgt gtctaaaacc tatgccatga
     1261 ctggttggcg gattggttat gccgttggag aagcagacat tatcgctgcc atgtccaaga
     1321 ttgcaggtca aacaacttcg aatccgtcag cagtagccca atatgcagca gttgaggctc
     1381 tatcaggtga gcaagatact gtagaaagca tgcgtcaggc ctttgaagaa cgtcttaata
     1441 ccatctatcc cctccttgca gaagtgccag gatttgaagt ggtcaaaccg caaggggcct
     1501 tctatctctt cccaaatgtc aaaaaggcca tggagatgaa aggctacacg gatgtgacag
     1561 actttacaac tgttatctta gaagaagccg aagtggcctt ggtaacagga gctggatttg
     1621 gagcaccaga aaatgtccgc ctcagctatg cgacagacct agacacgctt aaagaagcag
     1681 tcgaacgctt gaaagcattt atgggtagtg agaatgattg atcattttga gattaaggta
     1741 aaggatttac aaatttcaga aggattttat aggagttttc tcgctccttt ggactataaa
     1801 ttggctttta agactagttc tctaattagt tttctatccc cgaacagccc tcatcctggt
     1861 ggtgattttt ggctgactca gggaacacaa gatcctgttc attttgcttt tttagcagaa
     1921 aataaggaag aagttcaggc ttgttatgag gctggcttag aggcaggtgg gcgagacaat
     1981 ggggctcctg gttatcgaag tgagcatccg atttactatg ctgcttttat gattgacctg
     2041 gatgggaaca atatagaggt ggtttgccat aaagaataaa aaagaaaagg aaaaataatg
     2101 acaaaacgtg taacgattat tgacgtaaaa gactatgttg gtcaggaagt gacgattggc
     2161 gcttgggttg ccaacaaatc aggaaaaggg aaaatcgctt tcttacaatt gcgtgatgga
     2221 acagccttct ttcaaggtgt ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa
     2281 gtgggacttg agaagtttga tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg
     2341 acaggtattg tcaaagagga cgaacgttct aaatttggct atgagttgga catcacagac
     2401 atcgaagtga tcggtgaatc tcaagactac ccaatcacac caaaagaaca cggaacagac
     2461 tttttgatgg ataaccgtca cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa
     2521 atccgtaacg ctattatcta tgcaacttat gagttctttg acaagaacgg ttttatgaag
     2581 tttgacagcc caattctttc aggaaatgcg gcagaagatt ctacagaact ctttgaaact
     2641 gactacttcg gaacgccagc ctacttgagc caatcaggtc aactttacct agaagcaggg
     2701 gctatggctc ttggtcgtgt ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa
     2761 acacgccgtc acttgactga gttctggatg atggatgctg agtactcata cttgacacat
     2821 gatgagtcgc ttgacttgca agaagcttat gtgaaagctc ttctacaagg tgttcttgac
     2881 cgcgcgcctc aagccttgga aaccttggaa cgtgatacag aactcttgaa acgctacatt
     2941 gcagagccat tcaaacgtat cacttacgat caagccattg acctcttgca agagcatgaa
     3001 aatgatgaag atgctgacta cgagcatctt gagcatggtg atgactttgg gtcaccacac
     3061 gaaacttgga tttcaaacca ctttggtgtg ccaacatttg tcatgaacta tccagcagcc
     3121 atcaaggcct tctacatgaa accagttcct ggaaatccag agcgcgtgct ttgtgcagac
     3181 ttgcttgctc cagaaggcta tggagaaatt atcggtgggt ctatgcgtga ggaagattac
     3241 gatgcccttg tcgctaagat ggatgaactt ggcatggatc gtacagaata tgaattctac
     3301 cttgaccttc gtaaatacgg tacagttcca cacggaggat ttggtatcgg tatcgaacgt
     3361 atggtaacct tcgcagcagg aacaaaacat atccgtgaag ctattccatt cccacgtatg
     3421 ttgcaccgta tcaaaccata agaacaggaa aacgcccatg tggcgttttt tcgaattcta
     3481 gtgtttttac ttgcgtaaaa aaaatttttc tagtataata gtttattgtg agcgaacctc
     3541 acttacccct tgcaaagtct tggggtcatt agaccaaaag gaggaacata tcaatggcta
     3601 aatacgaaat tctttatatc attcgtccaa acattgaaga agaagctaaa aacgctttgg
     3661 tagcacgttt tgactctatt ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt
     3721 gggaaaaacg tcgtcttgca tacgaaatcc aagatttccg tgaaggactt taccacatcg
     3781 ttaacgttga agcaaatgac gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca
     3841 acgctgacat tcttcgtcac atgatcgtca aaattgacgc gtaagaaggt aaactatgat
     3901 taacaatgtt gtacttgtag ggcgtatgac acgtgacgct gaattgcgtt ataccccatc
     3961 aaatgtagca gttgcgactt ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg
     4021 tgaacgtgag gctgatttta tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc
     4081 taactgggct aaaaaaggct cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta
     4141 cgataaccag caaggacaac gtgtctacgt gacagaggtc gtggctgaga atttccaaat
     4201 gttggaaagc cgtagtgtgc gtgagggtca cacaggtgga gcttactctg caccaactgc
     4261 aaactattca gcacctacaa attcagtacc agacttttca cgtaatgaaa atccatttgg
     4321 agcaacaaac ccattggata tttcagatga tgatttacca ttctaatgga caactaaact
     4381 tataaaggag aaaaaacatg gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag
     4441 ttgattacat cgcagcaaac aaaattgaat atgttgatta caaagatact gagcttctta
     4501 gccgtttcgt ttcagaacgt gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta
     4561 aaaaccaacg taaagtaaca acagctatca aacgcgctcg cgtaatggct ttgatgcctt
     4621 tcgtaaacga agattaaaga aaagacccgg ttgggtctct ttttcatgaa cttgaaaaca
     4681 agaaagcgaa ttaagacccc aacgggtctt ttttcacaag ctttgaaatg agagagcgag
     4741 ttaggctcag tggctccctt tttacgagtt tgaaaacaag agaacacatt aaaagcacga
     4801 tggagctttt tcataaatat gtcttttttt tcatccaaga aattaaaata aaaatgataa
     4861 actttttgaa aataggggat ttgcctactt tatgatataa tggaaagagt tagattgaaa
     4921 gaggtagtga gatggacgcg aaattaagat acaaggcaaa gaagatcaag attgtctttt
     4981 ttgatattga tgatacattg cggaattcaa agacagggtt tattccaact acaattccca
     5041 ctgtttttaa acagttgcgt gaaaaaggaa ttttaacagg aatcgcctct ggacgtggca
     5101 tttttggtgt tgttccagag attcgtgatc tcaaacctga cttttttgta actttgaatg
     5161 gggcttatat cgaagataaa aaaggtcagg tcatttatca gcatcagatt gaaaagtcat
     5221 atgttgagga gtatatctct tgggctaagc aagaaggaat tgagtatggc ttggttggga
     5281 gtcatgatgc caagttgtcg actcgcaccg atatgatgag tgaagctatc aatccaattt
     5341 atcccgactt agatgtagat cccgatttcc atgaaaaaga agatatctat cagatgtgga
     5401 cttttgaaga taagggagat gacttgcact tgcctgacag tctctcagac aaacttcgca
     5461 tggttcgttg gcatcaacat tcgtctgata ttgtgccgat ttcaggctcc aaagcgacgg
     5521 gggtggaaaa ggttgtggaa caccttggct tgaaaccaga gaaggtcatg gtttttggag
     5581 atggtctcaa tgacttggaa ctctttgatt atgctggaat cagcgttgcc atgggaattt
     5641 ctcatgataa aatcaaagaa aaagcagatt atattacaaa aacattagaa gaagatggca
     5701 tttttgatgc cttagaagta tttggtatgg tagaaaaaga attgcatttt ccacaagtag
     5761 acattgaaac agtagaaggt cctcttgcga ctattaagac caatcacgga gacttacgta
     5821 tcaagctttt ccctgaacat gctcctaaaa cagtggctaa ctttgtatct ctttcaaaag
     5881 atggctacta tgatggtgtc attttccacc gtattatcaa ggactttatg atccaaggtg
     5941 gagacccaac tggaactggt atgggtggcg agtcaatcta cggcgaatct tttgaggatg
     6001 aattctcaga agagctttac aatatccgtg gtgctctttc catggcaaat gctggtccaa
     6061 ataccaacgg cagccagttc tttatcgtgc aaaaccaaca cctaccttat tctaagaaag
     6121 aaattactcg tggtggttgg ccagaaccga ttgcagaaat ctatgccaat caaggtggga
     6181 cacctcacct agaccgccgt cacacggttt ttggtcagtt agctgatgaa gcatcttacg
     6241 ctgtcttgga tgtcattgct gctgttgaga caggagctat ggacaagcca gttgaagatg
     6301 ttgtaattga aactattgaa atcgaggact aagatgaaaa tcggtgataa gctaaagggg
     6361 cgtattacag ggattcagcc ctacggtgcc tttgttgagt tagagacggg tgatacgggg
     6421 ctgattcaca tttcagaaat tcggacagga tttattgaaa atattcatga aaccttgaaa
     6481 gtcgatgaag aggttcaagt tcaggtagtg gatttagatg aatttacggg gaaagccagt
     6541 ctttctatcc gcactttgga ggaagaaaag taccagtttc ctagacggag acgcttttca
     6601 agcgaccgct ttaactacgg ttttgctccc tttcgacgaa tgctaccaat ctggactgga
     6661 gaagccctgc accatttaaa aaagaagaag tagtttagag ttttatattt tttgtaatgt
     6721 taatataaat ttgctataat aggaccatgg aagaagaaca attattaaaa tcaggagagc
     6781 gcattaacca gctcttttcg acagatatca aaatcattca aaatagagag gtttttagct
     6841 attcggtgga tagtgttctc ttatcacgat ttccacgttt tcctaagaag gggttgattg
     6901 tggatttctg tgctgggaat ggagcagtgg ggctttttgc tagcactcgt actcaagcac
     6961 agatattgtc tgttgagatt caggagcgtt tggcggatat ggctgaacgc tctgtccgtt
     7021 tgaatggatt agaggagcaa atgcaggtca tctgcgatga tttgaaaaat atgcctgctc
     7081 acattcaggg gagtaaggtg gatatgattt tgtgtaatcc accctatttc aaggtggatc
     7141 cttattctaa tctgaacgag agtgaacatt atctcttggc tcgacacgaa atcacgacta
     7201 atttggaaga aatctgtcat agtgcccaga gtattctcaa gtctaatggg cgtttggcca
     7261 tggttcatcg tcctgatcga cttctggata ttttggatac gttaaaacga cataatctag
     7321 cccctaagcg cctgcagttt gtttatccaa aaagagaaaa ggaagccaat atgcttttga
     7381 ttgaggcgat caaggatggc tcaacaagtg gctttaaggt cttacctcct ttgattgtcc
     7441 acaatgatga tggctcttat acgcccgaaa tcgaagagat ttattatgga tcataaggct
     7501 tatatgtacg ttctggagtg tcgtgatgga tcctactata taggctatac gactgatatg
     7561 agaagacgcc tcgctatcca caatagtggg aagggagcca aatatacacg agcacgcttg
     7621 ccagtcaaac ttatctatgc tcaaggtttt gccagtaagg aagaagccat gtcggctgaa
     7681 gctcttttca agcgtaagaa gaggccacag aaggaagaat ttttatctga aaatcaagat
     7741 agaaatttac tcagttattt tgaagaatcg tggggagtcc tttgactcct cttacctttg
     7801 tcaaaaagcg aaaaaaatgc tacaataaag aaaataaaca aaatgaggta ttatcatgtc
     7861 caagattcta gtatttggtc accaaaatcc agactcagat gccatcgggt catctgtagc
     7921 ttttgcctac cttgcaaaag aagcttacgg tttggatacg gaagctgttg cccttggaac
     7981 tccaaatgaa gaaacagcct ttgtcttgaa ctattttggt gtggaagcac cacgtgttat
     8041 cacttctgcc aaagcagagg gggcagagca agttatcttg actgaccaca atgaattcca
     8101 acaatctgta tcagatatcg ctgaagtaga agtttacggt gttgtagacc accaccgtgt
     8161 ggctaacttt gaaactgcaa gcccacttta catgcgtttg gagccagttg gatcagcgtc
     8221 ttcaatcgtt taccgtatgt tcaaagaaca tggtgtagct gtgcctaaag agattgcagg
     8281 tttgatgctt tcaggtttga tttcagatac ccttcttttg aaatcaccaa caacacaccc
     8341 aacagataaa atcattgctc ctgaattggc tgaattggct ggtgtaaact tggaagaata
     8401 tggtttggca atgttgaaag ctggtaccaa cttggctagc aaatctgctg aagaattgat
     8461 tgacatcgat gctaagactt ttgaactcaa cggaaataat gtccgtgttg cccaagtgaa
     8521 cacagttgac atcgctgaag ttttggaacg ccaagcagaa attgaagctg caatgcaagc
     8581 tgccaacgaa tcaaacggct actctgactt tgtcttgatg attacagata tcgtcaactc
     8641 aaactcagaa atattggctc ttggtgccaa tatggacaag gtcgaagcgg ctttcaattt
     8701 caaacttgaa aacaatcatg ccttccttgc tggtgccgtt tcacgtaaga aacaagtggt
     8761 acctcaatta actgaaagct ttaatgcgta agattttggg tgtcagctca aaatcggaaa
     8821 gtctagtttg ccttatatcg aaaggagttt cggctccttt ttttctagga gtgaagtatg
     8881 ttagaaaatg gcgatttgat ttttgtgaga gatgggtcag acatgggaca ggccatccag
     8941 acttccacag gtaactatag ccatgttgcc atttatttgg atgggatgat ttatcatgct
     9001 agtggacagg ctggtgctgt ctgtcaagaa ccggcagact tctttgagtc caatcatttg
     9061 tacgacctct atgtttaccc agaaatggat atccagtcgg tgaaggaaag agcttgcaga
     9121 catcttggag caccctacaa tgcttctttc tatccagatg cagctggttt ctactgctcc
     9181 cagtatatag cagaaatcct acctattttt gaaactattc ctatgaaatt tggagatggg
     9241 gaacaggaga ttagtgattt ttggagggag tattacatag aactaggtct gcctgttcct
     9301 ctgaaccaag ctgggaccaa tcctagtcag ttggcagcat cgcctctgtt acaatgtaaa
     9361 gaaaggaatc ttcatgattc agatttttaa ttcatctcgt ttgacgagac agccattttt
     9421 ggagaattga tccgctatct ggaccagtat gaggatgtga ttctacggga aattaaggct
     9481 caatttccag atgttgcagt tgataaactc atggaagagt atataaaggc aagcttgatt
     9541 ctacgtgaaa ataagcgcta ttacctcaat tttcctacgc ttgaatcact tgatagtctt
     9601 gaactggatc aagagatttt tgtcagagaa gctagtccgg tctatcaagc cttgttggag
     9661 cagagttttg agacggaatt gcgcaatcaa atcaatgcag ctattttagt tgaaagacgg
     9721 actttgcgcg cattaaaatg accctgtcca attattttta caaggtcaaa cagcagtatc
     9781 ctttgacaga aaaacagcag gagctctatg acattttagg agatgttaat cctgagtatg
     9841 cactcaagta tatgacggct tttttgttga aatttctcaa aaaagaccag cttatgcaga
     9901 aatgccgtga tatctttgtg gacagtttgg ttgtcttagg ctatattgtg caaaatgaag
     9961 atagaaagta tgagttggct atcgattttg ataaggagag attaactttc tacttagcgt
    10021 gatttcttgt ttctgagcac attgtttgac tttccttagt attcggtata aactatatgt
    10081 aaccggtaac acatatcgga ataaactaaa ggagacaatc atatgtcact tgaaaacaaa
    10141 ttggaacaag caacaggcgc tgtcaaagaa ggttttggta aagttactgg agacagcaag
    10201 acagaacttg aaggagctgt tgaaaaaaca gttgctaagg caaaagacgt tgtagaagac
    10261 gcaaaaggtg ctgtagaagg tgccgttgaa ggtttgaaaa acgtttttac taaataatag
    10321 gaaaaaatca agggtttcat tttcccttga ttttttctat tcttataaat aattttctgc
    10381 gacggctgta tctcctgggt aggattcttt cttgccctgg atgatttggt aacaatcggc
    10441 tcccttaccc gcaataataa ctgcatctaa ttcgtgattt gtgatagcca ttgccgcctt
    10501 gatggcttct tggcgatccg caatcttttc aacaggatga ttgatgtagc tactaatttc
    10561 atctgcaatg gccattgggt cttcatagtt agggtcatca gcagtcagaa agacttgaat
    10621 ctcagggtgt tgattgagga ggaggccaaa gtccttacga cgactttctc ccttgtttcc
    10681 tgttgatccc agaaccagag caatctttcc ggtttgatga gtttcaacca cattgatgag
    10741 ttttttcaga ctatccccat tgtgggcata gtcgatgaag accttggctc catttttctg
    10801 agtgaggact tccatacgac caggaacgcg ggttgcagcg atgccttttt tgatgtcctc
    10861 aagacttgct ccgagacgga gacaagcaag tccagcagca actgcatttt cttggttgaa
    10921 gttgccaatg agttggatat cataatctcc agcgagttta cccgtagctg aaaagctaaa
    10981 ggctttggaa ttctcgattt ggttatcaaa ttggctacca tagaaatcat ggtcttgatc
    11041 ttcaacctgt tctttcaaga ctgagaagtg gtccatgtca ctgttaatga tgactgctcg
    11101 gctattttcc atcaagagac gcttgtggta gaaatagtct tcaaagctag ggtgttcaat
    11161 cgggccgata tggtcaggag tgatgttaag aaagactcct acatcaaagg tcagtccata
    11221 gactcgatgg actagatagg cttgactgga gacttccatg atgaggtggg tacggtcatt
    11281 ttgcacagcc tgattcatca tgtcaaagag gtcaatactc tcaggggttg tcaacgctga
    11341 cttaaagaaa gtctcgccat caagagttgt gttcatggtc gacaacatag caggtctatg
    11401 cccttgagat aagatgttat aggcgaaata ggttgctgtt gtcttaccct tagtaccagt
    11461 aaaggcaagg agtttgagtt tctcttgtgg attaccatag aactccatgg caatcaaact
    11521 catggctttc tttatatcgt tcacaatgat gacagggata tcgacttcgt agtccttttc
    11581 agctacatac caagctaaac cttgtgttat agcagaaaga aggtattctt ttttaaaggc
    11641 agcgcctttt gcaaaaaaaa gagtgtcttc tgttactttt cggctgtcgt agctgatgct
    11701 atcaaaaata actttgctgt agttgtagtg gtaatgacct tggtcaataa tttcgcgaaa
    11761 aaggccatct ttctttaaaa tatctaatac ggtttcaatc ttaatcatac tttctattgt
    11821 aaaccgaaag tcgtaaattt acaagtaaca aggaaaagtt tataatggaa gataaggagt
    11881 ttttcctagt tatcaaaatt gaatgaggaa tctatgtcgc acgaaaacaa tcaccagcag
    11941 gcccagatgt tacgggggac tgcttggcta acggctagta actttatcag tcgcctactc
    12001 ggggctgttt acattatccc ttggtacatc tggatggggg cttatgcagc taaggcaaat
    12061 ggtctcttta ccatgggtta caatatctat gcttggttct tgttggtttc aacagcgggg
    12121 attccagttg cggtggccaa gcaagttgcc aagtataata ccatgcgaga agaagagcat
    12181 agctttgccc tgattcggag cttcttaggc tttatgacag gactaggcct ggtttttgct
    12241 ttagtcttgt atgtctttgc tccttggcta gcagacttgt ctggcgtggg caaagacttg
    12301 atcccaatca tgcaaagctt ggcttgggga gtcttgattt tcccgtctat gagtgttatc
    12361 cgaggatttt tccaagggat gaataacctc aaaccctatg ccatgagcca aattgctgag
    12421 caggtcattc gtgttatctg gatgctccta gcaaccttta tcattatgaa gctcggttca
    12481 ggagattatc tagcagccgt tacccaatca acctttgctg cctttgtcgg tatggtagcc
    12541 agttttgcag tcttgattta tttccttgcc caagaaggtt cactcaaaag agtctttgaa
    12601 acaggagata agattaacag taagcgtctc ttggttgata ccattaagga agccattcct
    12661 tttatcctga cagggtctgc catccagctc ttccagattt tggatcagct gacctttatc
    12721 aatagtatga gctggtttac caactacagc aatgaggact tggttgtcat gttttcttat
    12781 ttctcagcca atcctaataa aatcacgatg attttgattt ctgtaggggt ttcgattggg
    12841 agtgttggtt tgccactttt gacggaaaac tatgtcaagg gggacttgaa agcagcttct
    12901 cgtctcgttc aggacagtct caccctactc tttatgttct tgctaccagc aacggttgga
    12961 gtggttatgg taggagaacc tctttatacg gtcttctatg gtaagccaga tagtttggct
    13021 ctgggcttat ttgtctttgc agttttgcag tctattattt taggcttgta catggtcttg
    13081 tctccaatgc ttcaggccat gttccgcaac cgcaaggccg ttctctattt tatctatggt
    13141 tctattgcca agctagtctt gcaactacct accatcgccc tcttccacag ttatggtcct
    13201 ttgatttcaa caaccattgc tctcatcatt cctaacgtct tgatgtatcg ggatatttgt
    13261 aaagtaactg gtgtcaagcg caaggtgatt ttgaagcgaa ccattttaat cagtttgctg
    13321 accctagtca tgtttctgtt aataggaacc atccagtggc tgttaggatt tttcttccaa
    13381 ccaagtggac gtttgtggag cttcttttat gtagctcttg tcggtgccat ggggggtgga
    13441 ctttatatgg ttatgagtct gcgtacctat ttattagata aggtaatagg aaaagcccaa
    13501 gcagatcgcc tgcgagcaaa atttaagctt tcgtaaggta gcatttataa ataaaaggaa
    13561 taagttgaac aatcttttag gagaacttga agcttattcc ttttttagcg taaaaatagc
    13621 taaaattata tttcaaaaat agaaaatgag gcttttttcg attttttgga aaaaatcatt
    13681 gactaaacaa actaaacgtt gtataataaa acaaatattt aaagcaggag gttccggaaa
    13741 tgaaaaagtc taagagcaaa tatctaacct tggcaggtct tgtcctgggt acaggagttt
    13801 tattgagcgc gtgtggaaat tctagcacgg cgtcaaaaac atacaactat gtttattcaa
    13861 gtgatccatc tagcttgaac tatctagcag aaaaccgcgc agcaacatcc gatattgttg
    13921 caaatttggt agacgggtta ttagaaaatg accaatatgg gaatattatt ccatcattag
    13981 cagaggattg gactgtttct caggacggtt tgacctatac ctacaaactt cgtaaggatg
    14041 ccaagtggtt tacttctgag ggagaagaat atgcgcctgt aactgcccag gattttgtga
    14101 caggtttgca atatgcagct gataaaaaat cagaagcctt gtatctagtg caggactctg
    14161 ttgctggttt ggatgactat atcactggta aaacaagcga cttttcaact gtcggtgtca
    14221 aggcacttga tgaccaaacg gttcaatata ctttggttaa accagaactt tactggaatt
    14281 caaaaacact tgcaacgata ctttttcctg ttaatgcaga tttcctgaaa tcaaaagggg
    14341 atgattttgg gaaggcggat ccatctagta ttttgtacaa tggacctttc ttgatgaaag
    14401 cacttgtctc aaaatctgct attgaatata agaaaaaccc taattactgg gatgctaaga
    14461 atgtctttgt agacgatgtg aaattgacct actatgatgg tagcgaccaa gaatcactgg
    14521 aacgtaattt tacagctggt gcttatacta cggctcgtct ttttcctaac agctccagct
    14581 atgaagggat taaagaaaaa tacaaaaaca atatcatcta tagtatgcaa aattcaactt
    14641 catatttctt taattttaac ctagatagga agtcttacaa ttatacttct aaaacaagtg
    14701 acattgaaaa gaaatcgact caggaagcag ttctcaataa aaacttccgt caggctatca
    14761 attttgcttt tgacagaaca tcttatgggg ctcagtctga agggaaagaa ggtgcaacaa
    14821 agattttgcg taacctagtg gttcctccaa actttgtcag tatcaaggga aaagactttg
    14881 gtgaagttgt agcctctaag atggtcaact atggtaagga atggcaaggt atcaactttg
    14941 cggatggtca agacccttac tacaatcctg agaaagccaa ggctaagttt gcggaagcta
    15001 agaaagaact cgaagcaaag ggtgttcaat tcccaatcca cttggataag actgtggaag
    15061 taacagataa agtaggcata caaggagtta gttctatcaa acaatcaatt gaatctgttt
    15121 taggttctga taatgtagtg attgacattc agcaattaac atcagatgag tttgacagtt
    15181 caggctactt tgctcaaaca gctgctcaga aagactatga tttatatcat ggcggttggg
    15241 gacctgatta tcaagacccg tcaacctatc tcgatatttt taatactaat agtggaggat
    15301 ttctgcaaaa tcttggacta gagcctggtg aggccaatga caaggctaag gcagttggac
    15361 tggatgtcta tactcaaatg ttggaagaag ctaataaaga gcaagatcca gccaaacgtt
    15421 atgagaaata tgctgatatt caagcttggt tgattgatag ttctttagtt cttccaagtg
    15481 tttcgcgtgg gggaacacca tcattgagaa gaaccgtacc atttgctact gcctatggtt
    15541 taaccggtac aaaaggggtt gaatcatata aatacctcaa agtacaagat aagattgtca
    15601 caacagacga atatgcaaaa gccagagaaa aatggttgaa agaaaaagaa gaatccaata
    15661 aaaaagccca agaagaattg gcaaaacatg tcaaataaaa tctaattgta gataagtttg
    15721 tgtaagaagt ctttgtgctt aaaaacgcat aatatcaggt atataaaatc ctagatgtta
    15781 tgcgttttgt tataggaaga tttattagat tttatcctca aattgagtta ttcccgactt
    15841 tatccgttga aatattataa ggagtattta tgaaattaaa attattaaga gtagatacta
    15901 aggtgattat ggggagtttc ttacttgttc tgtctagtct acttgctttg ttgcttcccc
    15961 ttatcttaaa gggtttaata gatgggagtt ctattgaaaa tataggttcc aaagtatttc
    16021 aatcgttttt gatttttatt ggtcaagcct tgttttcttc tattggttac tatctgttta
    16081 gtcaatcggg tgaaaaaaag atagcaaaaa tcaggaaaaa agtgatagag gggttgattt
    16141 atgcagagaa atccttcttt gataagagcc aaagtgggga gttgacttct gccattgtca
    16201 atgacacgag tgtcattcgt gagtttttaa ttacgacttt cccaaatatt attctgagtt
    16261 tagttatggt acttggttcc attgtagtct tatttagtct tgattggaat ctttctctac
    16321 ttttattcat cactcttcct tgtatgatgt ttattatctt gctcctttcc aatatcagtg
    16381 aaaagtatag tcgtcgttta caggaggaaa tcggattttt aacagggcaa ttgactgaaa
    16441 agattcaaga acatgaatta attaagacta accaagcaga gaaaagtgta caggatgttt
    16501 tggataattg tattgaaagg gtacaaaaca attcactgaa atcagatagg gttacttctt
    16561 ttgagacccc gtttgctctc ttatttatct ttgcgactat agctgtgatg ctaacctatg
    16621 ggggttatcg ggtcagcgca ggatatatat ctgtgggaac cttggtttcg tttttgattt
    16681 acctctttca attacttaat cctattagta atatagctaa ttttgtaact gtttattcta
    16741 ggagcaaggg atcttcagtt gcactggaga acttgcttgc agttcctaaa gaaaaatttg
    16801 agtgaggaaa atcggtatca ggacaagggt tgaattttaa ccatgtctat tttggttatg
    16861 atgaaaatcg acctgtctta aaggatatta cttgttcaat tttcaagggg caaaaaattg
    16921 cttttgttgg accatctgga tcaggaaaat caacgattgt gcgtttgtta gagcggtttt
    16981 ataaaccgct ttcaggagat attctaatgg agcaatcaag tatatatgat tttaacttaa
    17041 aagaatggag aagtaaaatc gcttagtaac tgatgatgaa ttgatgaaag tgctagactt
    17101 agtatcacta ggtgatgaga ttcgctccat gaaagaggga ctagatactg aagttggtga
    17161 acgcggacga ctcttgtcag gggggcaaag ccaaagactt caaatagcta gagcctactt
    17221 aaaagatgct gaaattctta tatttgatga agccactgct aatcttgatg cggattctga
    17281 gtatgcgatt atcagtagcc tctattctgt attaaaggag aagacggttg tgattatagc
    17341 gcatcgtttg tcaacggtaa aagatgtgga ttgtattttc ttcttagagg aggggaaaat
    17401 cactggctca ggaactcata aggaactact ggaaaatcat gagcgttatg ctcgttttgt
    17461 gcaggagcaa atgatagagt gaaggtgtct tttgagattc accattttat agtctattaa
    17521 agggagcagg aaaaactccc tttttatata gtttgaaact ataactagct cttgaaaaga
    17581 agaaaatgag ttgatgaaaa taagtggtac aatagttact atagatttgg aggtattgta
    17641 tgagcaagga attacacatt aacacaattt tggcccaggc gggtattaag tcagatgaag
    17701 cgacaggtgc attggtgaca ccgcttcatt tttcaacgac ctatcagcat ccagagtttg
    17761 gtcgatctac tgggtttgac tatacgcgca ctaaaaatcc aactcgtagt aaggctgagg
    17821 aagtcttggc ggctattgag tcagcagact atgccctagc gactagctca gggatgtcag
    17881 ctattgtact ggcctttagc gtctttccag taggaagtaa ggtcttggca gtgcgtgatc
    17941 tttacggtgg ttcttttcgc tggtttaacc aagtggagca ggaaggtcgt ttccatttta
    18001 cctatgccaa tacagaagaa gagttgattg ccgagttaga aaaggatgtg gatgttctct
    18061 atatcgaaac cccaaccaat cccttgatgt tggaatttga tatcgaaaaa ctagcaaaat
    18121 tggctcatgc taagggtgcc aaagtggtgg tggacaatac cttctatagc cctatctacc
    18181 aacgtccgat tgaagatgga gcagatatcg ttctccattc agcaaccaag tatctagcag
    18241 gccacaatga tgtcttggct ggagtggttg tgaccaatag tttagaacta tacgagaagc
    18301 ttttttacaa tctcaataca acaggggcag tcttgtctcc atttgacagc taccagttgc
    18361 ttcgtggtct caagacctta tctcttcgta tggagcgctc aacagctaac gcccaagaag
    18421 tggttgcctt tttgaaggat tctccagcag ttaaggaagt tctctacact ggtcgtggag
    18481 gcatgatttc ctttaaagta gccgatgaaa cacgcattcc tcatattttg aacagtctca
    18541 aggtcttctc ttttgcggaa agtttgggcg gagtggaaag tcttattact tatccaacga
    18601 ctcaaactca tgctgatatt ccagcagaag tacgccattc ttatggtttg acagatgacc
    18661 tcttgcgttt gtctattggg attgaggatg ctagagattt gattgcagat ttgcgccaag
    18721 ccttagaagg ataagacaaa gatgggaaaa tatgatttta caagcctgcc caaccgttta
    18781 gggcaccata cctataaatg gaaagaaaca gaaacggata gtgaagttct accagcttgg
    18841 atagcggata tggactttgt ggtcttgcct gaaatccgcc aagccgtgca aacttacgca
    18901 gaccaactgg tttatggtta tacctatgcc agtgaagact taattaagga aattcaaaag
    18961 tgggaagcta cacaatacgg ttacaacttt gacaaagagg ctcttgtctt tatcgagggt
    19021 gtggtaccag ccatctcaac agctattcaa acctttacaa aagaaggcga ggcggtttta
    19081 attaacacgc ctgtctaccc accctttgct cgcagtgtca agttgaataa tcgtagattg
    19141 attactaatt ccttagtgga aaaggatggt ctgtttgaga ttgactttga ccaacttgaa
    19201 aaggatttgg tggaagagga ggttaaactc tatattcttt gcaaccctca caatcctggt
    19261 ggacgtgttt gggaaaaaga agtgttggag aagattggcc aactttgcca aaaacacggt
    19321 gttttgttag tttcggatga gattcaccaa gatttgaccc tctttggtca caaacaccag
    19381 tctttcaata ccatcaatcc tgccttcaaa aattttgcta tcgtcttgag cagtgccact
    19441 aaaacattta atattgctgg aacaaaaaat tcctatgcag tcattgaaaa tcctaagttg
    19501 agactggctt tccagaaacg cctgttggcc aataatcagc atgaaatttc aggcttgggt
    19561 tatttggcga cagaagctgc ctatcgatac ggtaaagatt ggctagagga actcaagcaa
    19621 gtctttgaag accacatcaa ttatgtggtg gatctatttg gaaaagagac taaaatcaag
    19681 gtcatgaaac cgcaaggtac ctacttgatt tggcttgact tttcagccta tgacctgact
    19741 gatgaaacat tgcaagagtt gttgagaaat gaagccaagg ttatcctcaa ccgtggtttg
    19801 gattttggag aggaaggaag tctccatgcc cgcatcaata tagctatgcc caaatctctg
    19861 ttgcaagaag tctgtcagcg gattgtggct acttttgcca aacgttaaaa atccagcctt
    19921 ctaggagaaa agtcttccta gaaggctatt ttcataggcg aaaatatggt ataataaaaa
    19981 gataaggtaa aggtgaaaat atggctaaat tgattccggg gaaaattcgt atcgaaggtg
    20041 ttgcccttta tgaaactggt aaggttgata tcatcaagga aaagaacaat cggctctacg
    20101 ctcgcgttgc agaagaagaa ctgcgctata gtttagagga tgatttggtt ttttgtgcct
    20161 gtgattcttt tcaaaagagg ggctactgtg tgcatttggc agcgctagag cattttctga
    20221 aaaatgatga gcgtggtcag gaaatcttgt ggagtctgga agaaggtcat gaagaaaaag
    20281 aggccgttga aaccaaggtg accttgggtg gcaagttttt gaatcgaatt ttatctccaa
    20341 aatcagaatg cgcctatgag ttatcagctg tgggtcaggt ggaagcagga accaatcata
    20401 tcttgtggac tctgagaatc ggccaaatca atagccaaaa atactatgtc attcgggata
    20461 ttccactttt tttaaggatt gtcgagcaga gaaagtcgta tatgattggc aagatttatg
    20521 aggagtctct ttcttgggaa gcctttgatg atgccagcca agaacttttg actttcttgc
    20581 acggactaac ggaggaaggg ctggttccag acctcttttt ccaaaaccaa ggtcggcacc
    20641 tctttttccc tctgaccttt tttgagcagg gtgtgaattt actgatggcc ttgccccatt
    20701 ttcaatttga ccatcaagtg gatagctacc aaaccctgct ttttcaggat atgcatgctg
    20761 atgccaatct atttgccttt acagtaacag aatactcgga ctattttgaa atggaaatca
    20821 gtgagagtcc tagggtcaat gtcttttatc agggagcagt acttttccat aaaggtcagg
    20881 tttatttttt aacagaccag cagatgcgtc ttctcaagga aattaaagcg ttacctgtgg
    20941 gtcaacaagg aaagaaatac ctgcaatttg atagcagtga tcgagacaaa ttggcctcct
    21001 gtctgactct ttttatccag atgggcaccg tttcagctcc agaacgtcta caaatcaaaa
    21061 cttttgcgcc ctctttttac tttgacaggg aagaagataa tcggattcgt ttagaaattc
    21121 agtttgatta tggaaataga caggtatcta gccgacaaga gctagaagaa ttaccatttt
    21181 caagtgacgc ggacttagaa gaaagaattt tccaagtctg tttggctgcg ggctttgaag
    21241 ccgattttca atcttggcgt caggccttaa aagcggagtc tgtctatcat ttttttcatg
    21301 atatcattcc agtctttgaa aaactcgggc atgttgacct atcagataaa ctagaagagc
    21361 tttatagcct agcgagtcct caggtgcaga ttgcctccaa gggaggtctc ttggaaatcc
    21421 agtttgattt tcaaaatatt gcccaggagg aaatcgacca agccatgcag gccttggttg
    21481 ccaatcagga tttttatatt gattcgtcta atcaagttta ctttttcgat gaagaaacca
    21541 agaaaattcg ccaaaatcta caggaactgg ggcaatttga attaaaagat gggaccttgc
    21601 aggctcggaa atccttggcc tacagtttag ctcatctctt tgaagggcga gaccgtgttt
    21661 ctttttcaca agaattccag aatctggccc aggatttgac gcatccagag gactttcctt
    21721 tgcaagcaac tcaggtcaag gctgacttgc gagattatca ggaaaaggga attggctggt
    21781 tgcagatgct ccatcattat ggttttggtg ggattttggc tgatgatatg ggacttggga
    21841 aaacccttca gaccattgct tttttgacta gtcaagtgac aaaagaaagt cgagtcttga
    21901 ttctcgctcc gtcgggtttg atttacaact gggcggatga gtttcagaaa tttgccccac
    21961 agttggatgt ggctgttgtt catggtttga aagcaagtcg tgaggagatt cttgccgaga
    22021 gccatcaaat ctatgtgact agctatgcca ccttccgtca ggacagtgag ttttatcagg
    22081 ggatggcctt tgacttcctt ttcttagatg aggctcaggt catgaaaaat gcccagacca
    22141 agattgccca gaccttgaga caatttgtgg tgccgtcggt ctttgccttg tcaggaactc
    22201 cgattgaaaa ccatctgggt gagttgtggt ctattttcca aatcgtcatg cccggacttt
    22261 tgccaagcaa gaaagaattt atgaaattgc cagcagagcg agtggctcag tttatcaagc
    22321 ctttcgtgat gcgacgcaag aaagaagaag ttctgactga attgccagac ttgattgagg
    22381 tggtttataa aaatgaactg gaagaccagc aaaaggctat ttaccttgcc cagttacaac
    22441 agatgcgaga ccatctggct caagtgtcag aacaggaatt tcagcgaagt cgtgtggaaa
    22501 ttttatctgg tttgatgcgc ttgcgtcaaa tatgtgacac tcctgccctg tttatggaag
    22561 attatcaggg agccagcggc aaactagata gtctccgaga cctactggta caggtggcag
    22621 acggcggaca ccgtgtcttg attttctctc agttcaaggg aatgttggaa aaaattgaac
    22681 aagaactgcc agacttgggc ctgacttcct ttaaaattac gggttcaacc ccagccaagg
    22741 aaagacaaga catgaccaag gcctttaatc aaggagaaag agatgccttt ctgatttccc
    22801 tcaaggctgg tggggtcggt ctgaacctga caggtgcaga tacagtgatc ttggttgacc
    22861 tttggtggaa tcctgcggtg gaagcgcaag ccattggccg tgcccatcgg atgggtcagg
    22921 aagaaacggt tgaggtctat cgcttggtga ccaagggaac cattgaagaa aaaattcagg
    22981 aactccaaga acaaaagaaa catctggtgt cacaagtatt ggacggcaca gagtcacgtg
    23041 gtagtctgac cctagcagaa attagagaaa ttttgggaat ttctgaagcc agcacttgaa
    23101 aaatcaagac aatacgctat aatgaagtgt tgaaaggaaa tagaaaatga aacaagaacg
    23161 atttccattg gtgtcagatg acgaggtcat gttgactgaa atgccagtca tgaatctcta
    23221 tgatgagtct gatctgatca gtaatatcaa gggtgagtat cgagataaaa attatttaga
    23281 atgggctcct attgctgaag aaaaaccagt aaaaccgatt gaaaagcaag tagggaaacc
    23341 taaaaaagct cctttagggg ttaaaaaaga aggaaagagc tatgcggagg tggcgcgtga
    23401 agaagcgcgt gcagacttga aaaagaaacg ctctgctaac tatctaactc aggatttcag
    23461 ccttgcgaga cgtcattctc agcccagtct agttagacag ggcaatcaac cgacaactcc
    23521 tttccaaaag gaaaatcctg gtgaatttgt caaatatagc caaaaattga cccagtctca
    23581 ttatatcttg gcggaagaag ttcattctat ccctaccaag aatgaagaag tgtcagcacc
    23641 tgctccaaag aaaaacaatt atgattttct aaagaagagc caaatctaca ataaaaaaag
    23701 taaacaaaca gaacaagaac gtcgggttgc ccaagagttg aatctgacca gaatgacaga
    23761 atagggagaa aacatgtcaa agacatatca ttttatcgga attaagggat cagggatgag
    23821 tgccttggcc ttgatgttgc accagatggg gcacaaggtt cagggatcag atgttgaaaa
    23881 gtactacttt acccaacgcg gtcttgagca ggcaggaatt accattcttc cttttgatga
    23941 aaagaatcta gacggtgata tggaaattat cgctggaaat gcctttcgtc cagataacaa
    24001 cgtcgaaatt gcctatgcgg accaaaatgg tatcagctac aaacgttacc atgagtttct
    24061 aggtagcttt atgcgtgact ttgttagcat gggagtagca ggagcacatg gaaaaacttc
    24121 aacgacaggt atgttgtctc atgttttgtc tcacattaca gataccagct tcttgattgg
    24181 agatgggaca ggtcgtggtt cggccaatgc caaatatttt gtctttgaat ctgacgaata
    24241 tgagcgtcac ttcatgcctt accacccaga atactctatt atcaccaaca ttgactttga
    24301 ccatccagat tatttcacaa gtctcgagga tgttttcaat gcctttaacg actatgccaa
    24361 acaaatcacc aagggtcttt ttgtctatgg tgaagatgct gaattgcgta agattacgtc
    24421 tgatgcacca atttattatt atggttttga agctgaaggc aatgactttg tagctagtga
    24481 tcttcttcgt tcaacaactg gttcaacctt caccgttcat ttccgtggac aaaacttggg
    24541 gcaattccac attccaacct ttggtcgtca caatatcatg aatgcgacag ccgttattgg
    24601 tcttctttac acagcaggat ttgatttgaa cttggtgcgt gagcacttga aaacattttc
    24661 cggtgttaaa cgtcgtttca ctgagaaaat tgtcaatgat acagtgatta tcgatgactt
    24721 tgcccatcat ccaacagaaa ttattgcgac cttggatgcg gctcgtcaga aatacccaag
    24781 caaggaaatt gtagcagtct ttcaaccgca tacctttaca agaaccattg ccttgttgga
    24841 cgactttgcc catgctttaa accaagcaga tgctgtttat ctagcgcaaa tttatggctc
    24901 ggctcgtgaa gtagatcatg gtgacgttaa ggtagaagac ctagccaaca aaatcaacaa
    24961 aaaacaccaa gtgattactg ttgaaaatgt ttctccgctc ctagaccatg acaatgctgt
    25021 ttacgtcttt atgggagcag gagacatcca aacctatgaa tactcatttg agcgtctctt
    25081 gtctaacttg acaagcaatg ttcaatagga tgttcccatg gaaattccaa ttaagatcat
    25141 tcaggcaagc aagtctgatt tgcctgagat aggggcactt cagacctcgt cttttccagc
    25201 tgaaaagcag caactttccc atattttaga agaaagtatc cgtaagtgtg cggatacctt
    25261 tcttctagct agggatgaaa atcaactttt aggctatatt ttatcaagtc cccagtcaga
    25321 caatccgcaa tatctaaaag tacattcttt agtcatcgag tctgaccatc agagacaggg
    25381 cttgggaaca cttcttcttg cagccttgaa agaggtggca gttgatctgg attacaaagg
    25441 gattcgtttg gagagtcctg atgagctgct ttcctatttt gaaatgaacg gttttgttga
    25501 tgaagaagca actttgctct atgcaactag ccagggctat agtatgattt ggtttaatcc
    25561 cttttatctg gaggaacaat gaaaatcaga caagcaagat tagaagattt ggatcggatt
    25621 gttgaacttg aatttgaaaa tttttcggtc gaagaagcca ttcctccttc tgtctttgaa
    25681 gcacatttga gagaaattca gacctctttt ctggttgctg aaaaagaagg aagaatcatg
    25741 ggttatatcg aaggaccagt tggcctgcac cgccatctgc aagaccagtc ttttacagaa
    25801 gaaataaaag actatagtca tgagcctggt ggttatatct ctgtgacctg tctgtctatt
    25861 gccaaggagg cacagggact cggactgggt cagaaattgc tgacagcttt gaaagaagtt
    25921 gctcttgaag atgagagaga tggcattaat ctaacctgtc atgactatct catcgcctat
    25981 tatgaaaaac atggatttgt caacgaaggc cagtcccagt caacctttgc aggggaaaca
    26041 tggtatgata tggtctggga aatgaaaaaa taagttagga aaagtatcat agattgcttt
    26101 ttttggactt ttaagatata atattatgga ttatcaacaa ggaggaaaaa cttttgagtg
    26161 aaaagtcaag agaagaagag aaattaagct ttaaagagca gattctgaga gatttagaaa
    26221 aagtaaaagg ctatgatgaa gttctgaaag aagatgaggc agtagttcgc actcctgcaa
    26281 atgaaccttc agctgaagaa ctcatgactg attccttgtc aacggtagag gagattatga
    26341 gaaaagctcc taccgtgcct actcacccaa gtcaaggtgt accagcttct ccagcagatg
    26401 agattcaaag agaaactcct ggtgttccaa gtcatccaag tcaagatgta ccttcttctc
    26461 cagcggaaga aagtggatca agaccaggtc ctgttagacc taagaaactt gaaagagaat
    26521 acaatgaaac cccaacaagg gtagctgttt cctatacgac ggcagagaaa aaagcagaac
    26581 aagcaggtcc agaaacacct acgcctgcta cagaaacagt ggatatcatc agcgatacat
    26641 cacgtcgtag ccgtagagaa ggagcaaaac ccgttaatcc taagaaagag aagaagtcac
    26701 atgtgaaagc ttttgtgatt tcattccttg tattccttgc cttgctctca gcaggtggtt
    26761 actttggtta ccagtacgtg ctagattctt tgttacctat cgatgctaat tctaagaaat
    26821 atgtgacggt tggaattcca gaaggttcaa acgttcaaga aatcggtacg acgcttgaaa
    26881 aagctggttt ggtaaagcat ggtctgattt ttagttttta tgccaagtat aaaaattata
    26941 ccgacttgaa agcaggttac tacaatttgc aaaagagtat gagtacagaa gacttactca
    27001 aagagttgca aaaaggtgga acagatgaac cgcaagaacc tgtacttgcg actttgacaa
    27061 ttccagaagg ttataccttg gatcagattg ctcaagctgt gggtcaattg caaggtgact
    27121 tcaaagagtc tttgacagcg gaggctttct tggctaaagt tcaagatgag acgtttatca
    27181 gtcaagcagt agcgaaatat cctactttac tggaaagttt gcctgtaaaa gacagcggtg
    27241 cgcgttatcg tttggaagga taccttttcc cagctacata ctctatcaag gaaagcacaa
    27301 ctattgagag cttgattgat gagatgttag ctgctatgga taagaaccta tctccttact
    27361 atagtactat caaatctaaa aatttgactg tcaatgagtt gttgaccatt gcttccttgg
    27421 tcgaaaaaga aggtgccaag acagaagatc gtaagctcat tgcaggtgta ttctacaatc
    27481 gtttaaatcg tgatatgcca cttcaaagta atattgcaat cttgtatgcc caaggaaaac
    27541 tggggcaaaa tatcagtcta gctgaggatg ttgcgattga taccaacatt gattcacctt
    27601 ataatgttta taaaaatgta ggtctcatgc ctggtccagt cgatagtcca agtctggatg
    27661 cgattgagtc aagcatcaat caaactaaga gcgataacct ctactttgta gcagatgtca
    27721 cagaaggcaa ggtctactat gctaacaatc aagaagacca cgaccgcaat gtcgctgaac
    27781 atgtcaacag caaattaaac taaacaaact aaaattatgt gatacgtcac ataattttct
    27841 ttagaaaata ttatcagaag aaagttgaga aaaatggcag aaaaaacata tcctatgacc
    27901 cttgaggaaa aggaaaaact tgaaaaagaa ttagaagaat tgaaattggt tcgtcgacca
    27961 gaagtggtag aacgcattaa gattgcccgt tcatacggtg acctttcaga aaacagtgag
    28021 tacgaagcag ctaaggatga acaagccttt gtcgaaggac aaatctctag cttagaaaca
    28081 aaaatccgct atgctgaaat cgtcaatagc gacgcagttg cccaggacga agtagcgatt
    28141 ggtaaaacag tcaccatcca agaaattggt gaggacgaag aagaagttta tattatcgta
    28201 ggttcagctg gtgcggatgc ctttgcaggt aaggtttcaa atgaaagccc aattgggcag
    28261 gccttgattg gcaagaaaac aggtgacaca gcaaccattg aaacgcctgt tggtagctat
    28321 gatgtaaaaa tcttgaaggt tgaaaaaaca gcctaaaaac agaaaaagga gtggggaggc
    28381 gatgtgcttc actcactcct ttttccattt tgctattctt cgaaaatctc ttcaaaccac
    28441 gtcagcgtcg ccttgccgta tgtatggtta ctgactttgt cagtttcatc ta
//
LOCUS       DAVBCG010000014        28401 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBCG010000014 DAVBCG010000000
VERSION     DAVBCG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.014"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            16..2169
                     /locus_tag="VQ495_000509"
     CDS             16..2169
                     /locus_tag="VQ495_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2530504.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2182..3531
                     /locus_tag="VQ495_000510"
     CDS             2182..3531
                     /locus_tag="VQ495_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2530505.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            3701..4408
                     /locus_tag="VQ495_000511"
     CDS             3701..4408
                     /locus_tag="VQ495_000511"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2530506.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            4610..8335
                     /locus_tag="VQ495_000512"
     CDS             4610..8335
                     /locus_tag="VQ495_000512"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2530507.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVTKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8428..9870
                     /locus_tag="VQ495_000513"
     CDS             8428..9870
                     /locus_tag="VQ495_000513"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2530508.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            9907..10929
                     /locus_tag="VQ495_000514"
     CDS             9907..10929
                     /locus_tag="VQ495_000514"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2530509.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            10926..11471
                     /gene="purN"
                     /locus_tag="VQ495_000515"
     CDS             10926..11471
                     /gene="purN"
                     /locus_tag="VQ495_000515"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2530510.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIALVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11555..12064
                     /locus_tag="VQ495_000516"
     CDS             11555..12064
                     /locus_tag="VQ495_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2530511.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12089..13636
                     /gene="purH"
                     /locus_tag="VQ495_000517"
     CDS             12089..13636
                     /gene="purH"
                     /locus_tag="VQ495_000517"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2530512.1"
                     /translation="MTKRVLISVSDKADIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYDDAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGENKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            13758..15020
                     /gene="purD"
                     /locus_tag="VQ495_000518"
     CDS             13758..15020
                     /gene="purD"
                     /locus_tag="VQ495_000518"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2530513.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFFEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLNYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15423..15911
                     /gene="purE"
                     /locus_tag="VQ495_000519"
     CDS             15423..15911
                     /gene="purE"
                     /locus_tag="VQ495_000519"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2530514.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            15898..16989
                     /gene="purK"
                     /locus_tag="VQ495_000520"
     CDS             15898..16989
                     /gene="purK"
                     /locus_tag="VQ495_000520"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2530515.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            16999..17226
                     /locus_tag="VQ495_000521"
     CDS             16999..17226
                     /locus_tag="VQ495_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2530516.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17289..18587
                     /gene="purB"
                     /locus_tag="VQ495_000522"
     CDS             17289..18587
                     /gene="purB"
                     /locus_tag="VQ495_000522"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2530517.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18642..22580)
                     /gene="strH"
                     /locus_tag="VQ495_000523"
     CDS             complement(18642..22580)
                     /gene="strH"
                     /locus_tag="VQ495_000523"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2530518.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEDDKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGSMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(22895..23611)
                     /locus_tag="VQ495_000524"
     CDS             complement(22895..23611)
                     /locus_tag="VQ495_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2530519.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            23959..25746
                     /locus_tag="VQ495_000525"
     CDS             23959..25746
                     /locus_tag="VQ495_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2530520.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            25743..26219
                     /locus_tag="VQ495_000526"
     CDS             25743..26219
                     /locus_tag="VQ495_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2530521.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26247..27152
                     /locus_tag="VQ495_000527"
     CDS             26247..27152
                     /locus_tag="VQ495_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2530522.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27139..27954
                     /locus_tag="VQ495_000528"
     CDS             27139..27954
                     /locus_tag="VQ495_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2530523.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            27961..28365
                     /locus_tag="VQ495_000529"
     CDS             27961..28365
                     /locus_tag="VQ495_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2530524.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
BASE COUNT         7743 a         5621 c         6708 g         8329 t
ORIGIN      
        1 gaaaaggaga tgaatatgaa atttgggaaa cgtcactatc gtccgcaagt ggatcagatg
       61 gactgcggtg tagcttcatt agccatggtt tttggctact atggtagtta ttattttttg
      121 gctcacttgc gagaattggc taagacgacc atggatggga cgacggcttt gggcttggtc
      181 aaggtggcag aggagattgg ttttgagacg cgagccatta aggcggatat gacgcttttt
      241 gacttgccgg atttgacttt tccttttgtt gcccatgtgc ttaaggaagg gaaattgctc
      301 cactactatg tggtgactgg gcaggataag gatagcattc atattgccga tccagatccc
      361 ggggtgaagt tgactaaact gccacgtgag cgttttgagg aagaatggac aggagtgact
      421 ctttttatgg cacctagtcc agactataag ccttataagg aacaaaaaaa tggtctgctc
      481 tcttttatcc ctatattagt gaagcagcgt ggcttgattg ccaatatcgt tttggcaaca
      541 ctcttggtaa ccgggattaa cattgtgggt tcttattatc tgcagtctat cattgatacc
      601 tatgtgccag atcagatgcg ttcgacacta gggattattt ctattgggct agtcatcgtc
      661 tacatcctcc agcaaatctt gtcttacgct caggagtatc tcttgcttgt tttggggcaa
      721 cgcttgtcga ttgacgtgat tttgtcctat atcaagcatg tttttcacct ccctatgtcc
      781 ttctttgcga cacgcaggac aggggagatc gtgtctcgtt ttacagatgc taacagtatc
      841 atcgatgcgc tggcttcgac catcctttcg attttcctag atgtgtcaac ggttgtcatt
      901 atttcccttg ttctattttc acaaaatacc aatctctttt tcatgacttt attggcgctt
      961 cctatctaca cagtgattat ctttgccttt atgaagccgt ttgaaaagat gaatcgggat
     1021 accatggaag ccaatgcggt tctgtcttct tctatcattg aggacatcaa cggtattgag
     1081 actatcaagt ccttgaccag tgaaagtcag cgttaccaaa aaattgacaa ggaatttgtg
     1141 gattatctga agaaatcctt tacctatagt cgagcagaga gtcagcaaaa ggctctgaaa
     1201 aaggttgccc atctcttgct taatgtcggc attctctgga tgggggctgt tctggtcatg
     1261 gatggcaaga tgagtttggg gcagttgatt acctataata ccttgctggt ttactttacc
     1321 aatcctttgg aaaatatcat caatctgcaa accaagcttc agacagcgca ggttgccaat
     1381 aaccgtctaa atgaagtgta tctagtagct tctgagtttg aggagaagaa aacagttgag
     1441 gatttgagct tgatgaaggg agatatgacc ttcaagcagg ttcattacaa gtatggctat
     1501 ggtcgagacg tcttgtcgga tatcaattta accgttcccc aagggtctaa ggtggctttt
     1561 gtggggattt cagggtcagg taagacgact ttggccaaga tgatggttaa tttttacgac
     1621 ccaagtcaag gggagattag tctgggtggt gtcaatctca atcagattga taaaaaagcc
     1681 ctgcgccagt acatcaacta tctgcctcaa cagccctatg tctttaacgg aacgattttg
     1741 gagaatcttc ttttgggagc caaggagggg acgacacagg aagatatctt acgggcggtc
     1801 gaattggcag agattcgaga ggatatcgag cgcatgccac tgaattatca gacagaattg
     1861 acttcggatg gggcagggat ttcaggtggt caacgtcaga gaatcgcttt ggcgcgtgct
     1921 ctcttgacag atgcgccggt cttgattttg gatgaggcga ctagcagttt ggatattttg
     1981 acagagaagc ggattgtcga taatctcatg gctttggaca agaccttgat tttcattgct
     2041 caccgcttga ctattgctga gcggacagag aaggtggttg tcttggatca gggcaagatt
     2101 gtcgaagaag gaaagcatgc tgatttgctt gcacagggag gcttttacgc ccatttggtc
     2161 aatagctaga aagaggagag gatgaaacca gaatttttag aaagtgcgga gttttataat
     2221 cgtcgttacc ataatttttc cagtagtgtg attgtaccca tggcccttct gctcgtgttt
     2281 ttacttggct ttgcaactgt tgcagagaag gagatgagtt tgtccactag agctactgtc
     2341 gaacccagtc gtatccttgc aaatatccag tcaactagca acaatcgtat tcttgtcaat
     2401 catttggaag aaaataagct ggttaagaag ggggatctct tggttcaata ccaagaaggg
     2461 gcagagggtg tccaagcgga gtcctatgcc agtcagttgg acatgctaaa ggatcaaaaa
     2521 aagcaattgg agtatctgca aaagagcctg caagaagggg agaaccactt tccagaggag
     2581 gataagtttg gctaccaagc cacctttcgc gactacatca gtcaagcagg cagtcttagg
     2641 gctagtacat cgcaacaaaa tgagaccatc gcgtcccaga atgcagcagc tagccaaacc
     2701 caagccgaaa tcggcaacct catcagtcaa acagaggcta aaattcgcga ttaccagaca
     2761 gctaagtcag ctatggaaac aggtgcttcc ttggccagtc agaatctagc ctactctctt
     2821 taccagtcct acaagtctca gggcgaggaa aatccccaaa ctaaggttca ggcagttgca
     2881 caggttgaag cacagatttc tcagttagaa tctagtcttg ctacttaccg tgtccagtat
     2941 gcaggttcag gtacccagca agcctatgcg tcagggttaa gcagtcaatt ggaatccctt
     3001 aaatcccaac atttggcaaa ggttggtcag gaattgaccc ttctagccca gaaaatcttg
     3061 gaggcagagt caggtaagaa ggtacaggga aatcttttag acaaggggaa aattacggcg
     3121 agtgaggatg gggtgcttca tcttaatcct gagaccagtg attctagcat ggttgcagaa
     3181 ggtgccctac tagcccaact ttatccatct ttggaaagag aagggaaagc caaactcaca
     3241 gcctatctaa gttcaaaaga tgtagcaaga atcaaggtcg gtgattctgt tcgctatact
     3301 acgactcatg atgccgggaa tcaacttttc ctagattcta ctattacaag tattgatgcg
     3361 acagctacta agactgagaa agggaatttc tttaaaatcg aggcggagac taatctaact
     3421 tcggagcagg ctgaaaaact taggtacggg gtggaaggcc gcttgcagat gattacgggc
     3481 aagaaaagtt atctacgtta ttatttggat caatttttga acaaagagta atgttcgtgt
     3541 ttttagagtt aaataatttt taaactgtga gaaagattct tcttgcagtt ttttctttac
     3601 aatttttaaa aaacatctgc tatttattcg gttaaattct tgtgtttttt ggttttttgt
     3661 ggtaaaatgt gctcaagtaa tacgaaaggc gaactttaaa atgtcaaaac aattgatcta
     3721 ttcgggaaaa gctaaagata tctatacaac tgaggatgaa aatcttatta tttcaactta
     3781 caaggaccag gcgactgctt tcaacggtgt caagaaggag cagattgcag gtaagggagt
     3841 cttgaataat cagatctcat cttttatttt tgagaaatta aatgcggctg gtgtggcgac
     3901 tcactttgtg gagaaacttt cagacacgga acaactcaat aaaaaggtta agattattcc
     3961 tttggaagtc gtgctccgca actatactgc tggttccttt tcaaaacgtt ttggtgtgga
     4021 tgagggaatc gccttggaga ctccgattgt cgaattttac tacaaaaatg atgatttgga
     4081 tgatccattt atcaatgatg agcatgtgaa attcctacag attgcgggtg accagcagat
     4141 tgcctacttg aaggaagaaa cgcgtcgtat caatgaacta ttgaaagtct ggtttgctga
     4201 gattgggctt aaattgattg actttaagct agagttcggt tttgacaagg atggcaagat
     4261 tatcttggca gacgaatttt caccagataa ctgccgcttg tgggacgctg atggcaacca
     4321 catggataag gatgttttcc gtagaggatt gggagaacta accgacgttt atgagattgt
     4381 ttgggaaaag ttgcaggaat tgaaataatc tgtttgcaac ggaaaacctt cgtctctcaa
     4441 ctaaaaggac tcaggctgaa aaggtccccc agaccttttc actctgtaga gaactaggtg
     4501 aactaacaga tgtttacgaa attgtctggg aaaagttgca gggtttaaaa taacaacctc
     4561 aaggctgttt gggaatattg caagagctga aataaaggaa taagaattga tggataaacg
     4621 tatttttgtt gaaaaaaagg ctgattttca ggtcaagtca gagagtttgg ttagagagct
     4681 ccagcacaac ttgggactgt caagcttgaa aagtattcgt attgtgcaag tatatgatgt
     4741 atttgacttg gctgaggact tgtttgcacc tgcagagaag cacattttct ctgagcaggg
     4801 aaccgaccat gttttagatg aagtatctgt gcaggcggat cttgctaact atgctttctt
     4861 tgccattgaa agtctgccag ggcagtttga ccagcgtgca gcttcgtcac aggaagcctt
     4921 gcttttgttg ggaagttcga gtgacgtgac agtcaacaca gcccaacttt acttggtgaa
     4981 taaagatatt gatgcgactg agttggaagc tgtcaaaaac tacctgctca atccagttga
     5041 ttctcgtttc aaggatatca cgacagggat tgccaagcag gagttttcag agtcagacaa
     5101 gaccattcca aaattgactt tctttgaaag ctatacagca gaagactttg ctcgctacaa
     5161 ggccgagcaa gggatggcca tggaagtgga tgatttgctc tttatccaag actactttaa
     5221 gtcaatcggg cgcgtgccaa ctgagactga actcaaggtt ttggacactt actggtctga
     5281 ccactgccgt catacgactt ttgagacaga gttgaaacac atcgactttt cagcttctaa
     5341 atttcaaaag caattgcagt caacctatga caagtatatt gccatgcgcg aggagttagg
     5401 tcggtctgaa aaaccacaaa ccttgatgga tatggcgact attttcggtc gttatgagcg
     5461 tgctaacgga cgattggatg atatggaagt ctctgacgaa atcaatgcct gctcagttga
     5521 aattgaagtg gatgttgatg gtgtcaagga accttggctc ctcatgttta aaaatgaaac
     5581 ccacaaccat ccaacagaaa ttgagccatt tggtggagcg gctacctgta tcggtggagc
     5641 tattcgtgat ccgttgtcag gccgttccta tgtttaccaa gccatgcgta tttcaggtgc
     5701 tggtgatatt acagcaccga tttcggaaac tcgtgctggg aaattgccac aacaagtcat
     5761 ttctaaaaca gcagctcatg gttattcttc atatggtaac cagattgggc ttgccacaac
     5821 ctacgttcgt gaatacttcc acccaggctt tgtagctaaa cgtatggaac ttggtgccgt
     5881 tgttggtgcg gctcccaagg gcaatgttgt ccgtgaaaaa cctgaagcag gtgatgtgat
     5941 catccttctc ggaggcaaaa caggtcgtga tggtgtcggt ggtgcgacgg gctcttctaa
     6001 ggttcaaaca gttgagtctg tagagactgc tggtgctgag gttcaaaaag gaaatgccat
     6061 cgaagaacgc aagattcagc gcctcttccg taatggcaat gtcactcgtc tgatcaagaa
     6121 gtccaatgac tttggggcag gcggcgtctg tgtggctatc ggtgaattgg cagacggtct
     6181 tgaaatcgac ctcaacaagg tgcctcttaa ataccagggc ttgaatggta cagaaattgc
     6241 catctctgaa tcacaagaac ggatggcggt cgtggttcgt cctgaagatg tggatacctt
     6301 cgttgccgaa tgtaacaaag aaaatattga tgctgttgtg gtggcgacag taactgaaaa
     6361 accaaatctt gtcatgcact ggaatggtga gacaatcgtt gacttggagc gtcgtttcct
     6421 tgacaccaat ggtgtgcgcg tggttgtcga tgccaaagtt gtggacaagg atgtcaaact
     6481 cccagaagag cgtcaaacat ctgctgaaac actggaatca gataccctta cggttctatc
     6541 tgacctcaac catgcaagtc aaaaaggatt acagactatc tttgactgct ctgttggacg
     6601 ctcaacggtt aatcacccac ttggtggtcg ttaccaactc acaccaactg aggcatctgt
     6661 gcagaaattg ccagttcaac acggtgtgac tcatactgcg tcggtcattg ctcaaggttt
     6721 caacccatat gtagctgaat ggtctccata ccacggtgct gcttatgcgg ttatcgaagc
     6781 aactgctcgt ttggtggctg ctggtgccaa ctggtctaag gctcgtttct cttaccaaga
     6841 atatttcgag cggatggaca agcaagcaga gcgtttcggt cagccagtag ctgctcttct
     6901 aggttctatt gaagcacaaa ttcagcttgg cttgccatct atcggtggta aggactccat
     6961 gtctggtacc tttgaagaat tgaccgttcc gccaaccttg gttgcctttg gggtgacgac
     7021 ggcagatagc cgtaaggtgc tctctccaga atttaaagct gttggggaaa atatctacta
     7081 catcccaggt caagccctct ctgcagagat tgattttgac ttgattaaga aaaattttgc
     7141 tcagtttgaa gcaagccaag ctgaccataa agtgacgtct gcatcagctg tcaaatacgg
     7201 tggtgtagtt gaaagtttgg ctcttgctac ctttggaaac tatattggtg cagaggtgac
     7261 cttgcctgaa cttaaaacag ctttgacagc tcaattaggc ggctttgtct tcacatctcc
     7321 tgaagaaatt gctggagtag agaagattgg acaaacgaaa gcagacttta cactgactgt
     7381 caacggtgtg aagctagatg gacacaagct tgacagtgca tttcaaggga cattggaaga
     7441 agtttaccca acagaattta cccaagcgaa agaactagaa gaagtaccag ctgtggcatc
     7501 agatgttgtg attaaagcca aagaaaaggt tgaaaaacct gtggtttaca tcccagtctt
     7561 tccaggaacc aactcagaat atgattcagc taaggccttc gaaaaagaag gtgcagaggt
     7621 caatttggtg ccattcgtga ccttgaatga agaagctatt gtcaagtcag ttgaaactat
     7681 ggttgacaat atcgacaaga ctaatattct cttctttgcc ggtggattct cggctgcgga
     7741 tgaaccagat ggttcagcta agtttatcgt caatatcctg cttaatgaaa aagtgcgtgt
     7801 ggctattgat agctttatcg cccgtggtgg cttgattatc ggtatttgta atggattcca
     7861 agccttagtc aaatcgggtc tcctaccata cggaaacttt gaagatgcta acagtactag
     7921 tccaaccctc ttctacaatg atgccaacca acacgtgacc aagatggtgg aaactcgcat
     7981 tgccaatacc aactcaccat ggttggctgg tgtgcaagtg ggcgatatcc acgctattcc
     8041 tgtttcgcac ggtgaaggga agtttgtcgt gacggctgag gaatttgcag agctccgtga
     8101 caatggacaa attttcagcc aatacgttga ctttaacggt aaaccaagta tggattctaa
     8161 gtacaatccg aatggttctg tccatgccat cgaaggaatt accagcaaga atggtcaaat
     8221 catcggtaag atgggccact cagaacgtta tgaggatggt cttttccaaa atatcccagg
     8281 caataaagac caacacctgt tcgcatcagc ggttaaacat ttcactggaa aataagactt
     8341 acagattttc taatagatag tatcagtaat gtaaaagtca tgtaaatcta gctcttgatg
     8401 attacaaatg aaaattaggt ataaaaaatg acatacgaag taaaatctct taatgaagaa
     8461 tgtggtgttt tcggtatttg gggacatcca gatgctgcta agttgaccta ttttggactc
     8521 cacagtcttc aacaccgtgg tcaggagggg gcaggaatcc tctccaatga tcaaggacaa
     8581 ctgaagcgcc atcgtgacat ggggctttta tcagaagttt tcagaaatcc agccaatttg
     8641 gataaattga caggagctgg tgcgattggg catgtgcgtt atgcgactgc tggcgaagct
     8701 tctgttgata acatccagcc cttcctcttc cgttttcacg atatgcagtt tggcttggct
     8761 cataatggaa atctgaccaa tgcagcctct ctcaagaaag aactggaaca aagaggagca
     8821 attttcagcg cgacttcgga ctctgaaatc ttggctcacc tcattcgtcg tagtcataat
     8881 cctagcctga tgggcaaaat caaggaagcg ctcagccttg tcaaaggtgg ttttgcctat
     8941 atcttgctgt ttgaggacaa gttgattgcg gctcttgacc ctaatggctt tcgtccgctt
     9001 tctatcggga aaatggccaa cggagcggtg gttgtttcct ctgaaacctg tgcttttgag
     9061 gtcattggtg ccgagtggat tcgtgatttg aagccaggtg agattgtgat cattgatgac
     9121 gagggcattc agtatgacag ctatacagat gatactcagt tggcgatttg ttctatggag
     9181 tatatctact ttgctcgccc tgattctaat atccacggtg tcaatgtcca tacggcacgt
     9241 aagagaatgg gagcgcaatt ggcgcgagaa tttaagcatg aggcagatat tgtagttggt
     9301 gtgcccaatt cttccctaag cgcggctatg ggatttgcgg aagaatcagg cttaccaaat
     9361 gaaatgggtc tgatcaaaaa ccaatacacc cagcgaactt ttatccaacc gactcaagaa
     9421 ttgcgggagc aaggagtgcg gatgaaactg tctgctgttt cgggtgttgt caaaggcaaa
     9481 cgtgtggtca tggtggatga ttccattgta cgtggaacaa cctctcgtcg tatcgttcag
     9541 ctcttgaaag aagcgggtgc gactgaggtt cacgttgcca ttggaagtcc tgcactagcg
     9601 tatccatgtt tctacgggat tgatatccag acccgtcagg agctgattgc agccaatcat
     9661 acggtcgaag aaactcgcca aatcattggt gcggacagtc tgacttatct ttcaattgat
     9721 ggcttgattg agtcgattgg tatcgaaaca gatgcgccga acggtggtct ctgtgtcgct
     9781 tactttgacg gtgactaccc aacgcctctc tatgactacg aagaagacta tcgtagaagt
     9841 ttggaagaaa agaccagttt ttacaagtag gcgacagatt ctccattaaa gaaaaggaaa
     9901 aaacaaatga caaataaaaa tgcatatgcc cagtcgggtg tggatgttga agcgggttat
     9961 gaagttgttg aacggattaa aaagcacgtg gcccgtacgg agcgtgcagg tgtcatggga
    10021 gctcttggtg gctttggtgg tatgtttgac ctttccaaga ctggggttaa agaacccgtc
    10081 ttgatttcag ggactgacgg tgtcggaacc aagctcatgt tggctatcaa gtacgacaag
    10141 cacgatacca tcgggcagga ctgtgtggcc atgtgtgtca acgacatcat tgctgcaggt
    10201 gcggaacccc tctattttct cgactacgta gcgacaggga agaatgaacc agctaagcta
    10261 gaacaagtgg ttgctggtgt ggcagaaggt tgtgtgcagg ctggtgctgc cctcatcggt
    10321 ggggaaacgg ctgaaatgcc gggcatgtac ggcgaagacg actatgactt ggctggtttt
    10381 gcggtcggtg tggctgaaaa atctcaaatc attgacggtt caaaggtggt agagggagat
    10441 gttcttctcg gacttgcttc aagtgggatt cactcaaatg gttactcttt ggttcgtcgt
    10501 gtctttgcgg attacacagg tgaggaagtc ctaccagaat tggaaggcaa gaaacttaag
    10561 gaagttctac ttgagccgac tcgtatctat gtcaaggctg tcttgccgct catcaaagaa
    10621 gagttggtca acggcattgc ccacatcaca ggtggtggct ttatcgaaaa tgtccctcgt
    10681 atgtttgcag atgacctagc tgctgaaatt gatgaaagca aagttccagt gcttccaatt
    10741 ttcaaagccc ttgaaaaata tggtcagatt aaacacgaag aaatgtttga aatcttcaat
    10801 atgggtgtgg gacttatgtt ggcggtcagc cctgaaaatg tagagcgtgt aaaagaattg
    10861 ttggatgaag cagtctatga aattggtcgc atcgtcaaga aagaaaacga aagtgtcatt
    10921 atcaaatgaa aaaaatagcg gtttttgcct ctggtaatgg ctcaaatttt caggtgattg
    10981 ccgaagaatt tccagtggag tttgtctttt cagaccatcg tgatgcctat gtgcttgagc
    11041 gtgcaaagca gctcggcgtt ctgtcctatg cttttgaact caaggagttt gagagcaagg
    11101 cagactacga agcagccctt gtcgaactct tggaagaaca ccagattgcc ttggtttgcc
    11161 tagcaggcta catgaaaatc gttggaccaa ccttattgtc ggcttatgaa ggtcggattg
    11221 tcaacattca tccagcctac ttgccagaat ttccaggagc tcatgggatt gaggatgctt
    11281 ggaatgctgg cgtgggtcag tctggtgtga ccattcactg ggtggattcg ggtgtggata
    11341 caggccaggt catcaaacag gttcgtgtgc cacgactagc tgatgatacc attgacagat
    11401 ttgaagctcg catccatgaa gcagagtaca ggttgtatcc ggaagtagtg aaggctctat
    11461 ttacagattg actttttgat gattcatatg atatctttga ttttaaattg gagtcagtgt
    11521 ttgttgaaga cggcttcaaa cggaggtatt tgtaatgtta gaatctaaaa aaacaactcg
    11581 atatgtattt tatgtctatc tgatgttatt aacttgggga atcttattta agtttgaaac
    11641 aaatcctgaa tttatagcat ttttcttagc tccaaggtat atcaattgga ttccattttc
    11701 agaaccacta atagtcgatg gaaaaattgt ttttgctgaa atgttattta atctgatttt
    11761 ctttattcca ttaggtgttt gtttcccttt gataaaaact aatttatcta gtttaagaat
    11821 agtcgggaca ggtttcttga ttagtttatt gtttgagtgc ttacagtata ttttagcaat
    11881 aggtataaca gatataacgg atttgacttt aaatacgcta ggtgtctgtg taggcttact
    11941 gatttatcaa atttttataa gagtgttcaa atcacagact agaaaatgga tcaatatctt
    12001 aggtatgctt agccttggtt ttgcttatct tgttttactg ttactgcatt taattggtgt
    12061 ttaactaatg attaaaaagg agaatataat gactaaacgc gtcttaatca gcgtctcaga
    12121 caaagcggac attgttgaat ttgcccaaga actcaaaaaa cttggttggg agattatctc
    12181 gacaggtgga actaaggttg cccttgataa tgctggggtg gataccattg ctatcgatga
    12241 tgtgactggt ttcccagaaa tgatggacgg tcgtgtgaag accctccacc caaatatcca
    12301 cggagggctt ctcgctcgtc gtgacttgga tagccacttg gaagcggcta aggacaacaa
    12361 gattgagctc attgaccttg tggtggtcaa cctttaccca tttaaggaaa ctatccttaa
    12421 accagatgtg acttatgatg atgcagttga aaatatcgat attggtgggc catctatgct
    12481 tcgttcagca gcgaaaaatc atgccagtgt tacagttgtg gtagatcctg ctgactacgc
    12541 tgtggttttg gacgaattgg cagcaaacgg cgaaacctct tatgaaactc gccaacgttt
    12601 agcagccaaa gtatttcgtc acacagcggc ttatgacgcc ttgattgcag aatacttcac
    12661 agctcaagtg ggtgaaaaca agcctgaaaa actcactttg acttatgacc tcaagcaacc
    12721 aatgcgttac ggtgagaatc ctcaacaaga cgcggacttt taccagaaag ctttgcctac
    12781 agactactcc attgcttcag ccaaacagct caacgggaaa gaattgtcat ttaataatat
    12841 ccgtgatgca gatgctgcta tccgtatcat ccgtgacttc aaagatagtc caaccgttgt
    12901 ggctctcaaa cacatgaatc catgtggaat tggtcaagct gatgacatcg agactgcttg
    12961 ggactacgct tatgagtctg acccagtgtc tatctttggt gggattgtcg tcctcaaccg
    13021 tgaggtggat gctgcgacag ctgagaagat gcacggcgtt ttcctcgaaa tcatcattgc
    13081 accaagctat acggatgaag cgctagccat tttgatcaat aaaaagaaaa acttgcgtat
    13141 ccttgccttg ccatttaatg ctcaagaggc tagcgaagtg gaagcagaat acacaggtgt
    13201 agtcggtgga cttctcgtgc aaaatcaaga cgtggtcaag gaaagcccag ctgactggca
    13261 agtggtgact aaacgtcagc caactgagac agaagcgact gctcttgagt tcgcttggaa
    13321 ggctatcaag tacgtcaaat caaatggtat tatcgtgacc aacgaccaca tgacacttgg
    13381 tgttggtcca ggtcaaacca accgtgtggc ttctgttcgc cttgccattg accaagccaa
    13441 agatcgtctg gacggggcgg tccttgcttc agatgccttc ttcccatttg cggataacgt
    13501 ggaagaaatc gccaaagcag gaattaaggc catcatccag cctggtggct ctgtccgtga
    13561 ccaagaatcc atcgaagcag cggataaata cggcttgact atggtcttta caggtgtgag
    13621 acattttaga cattaagaag ataaaaggga agaaaacagt ttctttcctt ttttggctta
    13681 aaatgctaac tgaaacaaga ttaaaacgaa cttttttgat ataatgttgg taaataattc
    13741 gcaaaagagg ttgaggaatg aaactgcttg ttgtcggttc tggtggtcgt gagcatgcga
    13801 ttgctaaaaa gttacttgaa tcaaaagacg tggaaaaagt ctttgtagct cctgggaatg
    13861 atgggatgac tctggatggt ttggaattgg taaatatctc tatttccgaa cattctaaat
    13921 tgattgactt cgcaaagacc aatgatgttg cttggacctt tatcggtcca gatgatgccc
    13981 ttgctgctgg tatcgtggat gattttaacc aagctgggct taaggccttt ggtccgacta
    14041 gggctgcagc ggagctggag tggtccaagg attttgccaa ggaaatcatg gtcaaatatg
    14101 gtgtttcgac agcagcctat ggcacatttt cagatttcga ggaagcaaag gcctatatcg
    14161 aaaagcatgg tgcgcctatc gtagtcaagg cggatggctt ggcgcttggg aagggtgtcg
    14221 tcgtttctga gacggttgag caagcggtcg aagccgctca tgagatgctt ttggacaata
    14281 aatttggtga ctcaggtgcg cgcgtggtta ttgaggaatt ctttgaagga gaggaatttt
    14341 cactctttgc ctttgtcaat ggtgataagt tctacatcat gccaacggct caggaccaca
    14401 aacgtgccta tgatggcgac aaagggccta acacgggtgg tatgggtgcc tatgcgccag
    14461 ttccacactt accacagagt gtagttgata cagcggttga caccattgtc aagccagttc
    14521 tagaagggat gattaaagaa ggtcgccctt atctgggagt tctttacgca gggcttatcc
    14581 tgacagctga tggaccgaaa gtcattgagt tcaacgctcg gttcggagat ccagaaactc
    14641 agattatctt gcctcgctta acctctgact ttgctcaaaa tatcacagat atcctggata
    14701 gcaaggagcc aaatatcacg tggacggaca agggtgtgac tctgggtgtg gttgtcgcat
    14761 ccaagggcta cccgctaaat tattcaaagg gtgttgagtt gccagtcaaa accgatggcg
    14821 acatcattac ctactatgca ggggctaagt ttgcggaaaa tagcagagca ctgctctcaa
    14881 acggcggacg agtttatatg ctcgttacca cagcagatac cgtcaaagaa gcccaagcca
    14941 gcatctacca agaactatcc caacaaaaaa tagaaggact cttctaccga acagatatcg
    15001 gaagcaaggc aattaagtaa agatataaga ataacgcgcc gtagtcgcca aacacgataa
    15061 tggtcgtcgt ggtgaaaaga ccagaacagt gaatgttctg gtcaggggga aacttggaga
    15121 ccgtaggctc aaagtttagg aatgaaaccg aaggtttgct tccgcctcca tcacctaaga
    15181 ccattatcaa aaagaaaaat aaaaattcac aaaatacgtt aatgatcgta tggtttgcga
    15241 gcgttagcga gctaatatag aacaatcacc gccgttgtga aagaacgatt ggatgataat
    15301 ccaatcgttc agggaaattg gaagaccttg ggtttccaat ttaggcatga gacacctttg
    15361 gtggctgctg ccgtccctca caagctaagg tgattgttga aaaagaggaa aaaggagaag
    15421 aaatgaaacc agtaatttcc atcatcatgg gctcaaaatc cgactgggca accatgcaaa
    15481 aaacagcaga agtcctagac cgcttcggtg tagcctacga aaagaaagtt gtttccgcac
    15541 accgcacacc agacctcatg ttcaaacatg cagaagaagc ccgtagtcgt ggcatcaaga
    15601 tcatcatcgc aggtgctggt ggcgcagcgc atttgccagg catggtagct gccaaaacaa
    15661 cccttccagt cattggtgtg ccagtcaagt ctcgtgctct tagtggagtg gattcactct
    15721 attctatcgt tcagatgccg ggtggggtgc ctgttgcgac catggctatc ggtgaagctg
    15781 gagcgactaa cgcagctctc tttgccctcc gtctcctctc tgtagaagat aagtccattg
    15841 cggatgcact tgccaacttt gctgaagaac aaggaaaaat cgcagaggag tcgtcaaatg
    15901 agctcatcta aaacaatcgg aattatcggt ggcggtcaac tgggtcagat gatggccatt
    15961 tctgctatct acatgggcca caaggttatc gcgctggatc ctgcggcgga ttgcccggcc
    16021 tctcgcgtgg cggaaatcat tgtggcacct tataacgatg tggacgccct ccgtcagttg
    16081 gcagaccgtt gcgatgtcct cacttatgag tttgaaaatg tcgacgctga cggtttggat
    16141 gccgttatca aggatggaca actccctcaa ggaacagatc tgctccgcat ttcgcaaaat
    16201 cgtatttttg aaaaggactt tttgtcaaac aaggctcaag tcactgtggc accctacaag
    16261 gtcgtgactt ctagcctaga cttggcagat atcgacttgt cgaaaaacta tgtcctcaag
    16321 actgcgactg gtggctacga tggtcatgga caaaaggtta ttcgttcaga agcagacttg
    16381 gaagcagcct atgcgctagc agactcagca gactgcgtct tggaagaatt tgtcaacttt
    16441 gaccttgaga tttctgtcat cgtgtcagga aatggcaagg aggtgacgtt tttcccagtt
    16501 caggaaaata tccaccgcaa caatatcctg tctaagacca tcgtaccagc ccgcatttct
    16561 gaaagtctag cagacaaggc taaagctatg gcagtgcgaa tcgcagaaca actcaacttg
    16621 tctggaactc tctgtgtgga aatgtttgcg actgatgatg acgttattgt caatgaaatc
    16681 gccccacgac cacataactc tgggcactat tctattgaag cctgtgattt ctctcagttt
    16741 gacacccata ttctgggtgt tctcggagca ccattaccag tcatcaaact ccatgcgcca
    16801 gccgttatgc ttaatgtcct cggtcagcat gtcgaggctg ctgagcaata tgtgacagaa
    16861 aattcaagcg cccacctcca catgtatggt aaaatagaag cgaagcacaa ccgcaagatg
    16921 ggacatgtga ccttgtttag tgatgtaccg gatagtgtgg atgagcttgg ggaagggatt
    16981 gatttttagg acaagtctat gatacaaatt atcgttaata catttattga aaaggataag
    17041 actggagcag ttgttgaagt gttgtatgcc agtgctgacc aagataaggt acaagctaaa
    17101 tatgaagaac tagctgcaca ataccccgaa aattatttag ctatctatga tgtaccgctg
    17161 gatacggatt tgaatacact agatcattac ccgtctgtgt ttattgaaaa agaggagttt
    17221 gagtagaaat cttggtttac ctagatagct tattcccaac agcttaagaa gaaaggaaaa
    17281 attaacacat gatcaaccgt tactctcgcc ctgagatggc gaatatttgg agtgaagaaa
    17341 ataaataccg tgcttggctt gaggtggaaa tcctctctga cgaggcatgg gctgagttgg
    17401 gggaaatccc taaggaagat gtggctttga ttcgcaagaa ggcggacttt gacatcgacc
    17461 gtattttgga aattgagcag gagacgcgcc acgatgtggt ggctttcacg cgtgcggttt
    17521 ctgagactct tggtgaagag cgcaagtggg ttcactatgg gttaacttct accgacgtgg
    17581 tggatactgc ttatggttac ctctacaagc aggccaacga catcatccgt cgtgaccttg
    17641 aaaacttcac taatatcatc gctgacaagg ccaaggagca caagttcacc atcatgatgg
    17701 ggcgtactca tggtgtgcac gctgagccga caacctttgg tcttaaattg gcaacttggt
    17761 acagcgaaat gaaacgcaat atcgagcgtt tcgagcatgc agctgctggt gtagaagctg
    17821 gtaagatttc tggtgcggtt gggaactttg ccaatatccc accatttgta gaggagtatg
    17881 tctgcgataa acttggcatc cgtgcccaag aaatctctac acaagtcctt cctcgtgacc
    17941 ttcacgctga gtactttgcg gttcttgcca gcattgcgac ttcaatcgaa cgtatggcga
    18001 ctgagattcg tggtctacaa aaatctgagc aacgcgaagt agaagagttc tttgctaaag
    18061 ggcaaaaagg gtcttcagca atgcctcaca aacgcaaccc aatcggttct gaaaatatga
    18121 ctggtctggc gcgtgtcatt cgtggtcaca tgattacggc ttatgaaaac gtcgctctct
    18181 ggcacgaacg cgatatttct cactcatcag ctgagcgtat catcacacca gatacgacca
    18241 ttttgattga ctacatgctc aaccgttttg gaaatatcgt caagaacttg acagtcttcc
    18301 cagaaaatat gatccgaaac atgaactcga cttttggtct tatctttagc caacgggcta
    18361 tgttgacatt gattgaaaaa ggcatgaccc gtgagcaagc ctatgacttg gtgcaaccaa
    18421 aaacagccta ctcttgggac aaccaagtag actttaaacc acttcttgag gcagattcag
    18481 aagtaacatc acgtctcact caagaagaaa tcgatgaaat cttcaaccca gtttattaca
    18541 ccaaacgagt ggatgatatc tttgaacgtc ttggactagg tgattaatta aaaaataaac
    18601 agcgagcttc aatctcgctg tttatttttt atcgaaaaga cttagtcttc ttttctttta
    18661 gtgagtccat aggctgctag tgtggccatg agtcctgcga ctactagtcc tgcagaatcg
    18721 tgagttcctg tttcaggaag ttttttctct attaccacag gagctggatc ttgaggaaga
    18781 gctttgcttt cctcagcagg agcagttgat ggagctggtt ggcttgggat ttctagtttt
    18841 ggtttttctt cagcaatagc ggcttgtccg ttttcatcgc ctacatgtgt taccatagtt
    18901 ccgacttcga ctatttgagt aacggcttcc tgtgctacga cactatttac aagtgttttc
    18961 acttccttac catcggcaga agtgctcaca gagtagaagt tgctacgatg tccattgacg
    19021 cccttagtaa tgacttgtgt ttttcctttg agtaagagtg gattttcacg agtcactgtg
    19081 gtaaatggaa tttcttcttt ttggatatcc agtctaggtt ttacctcagt agttggtgca
    19141 agaccacttt catcaccctt gtgagttaca ggagcgccaa cttcaaccac ttggtttata
    19201 acttctttgg ttacctggct atcaaggact gtttctgttg tttttccatt ttcagtgagt
    19261 acagagatgt aatgagttcg ctcacctttg actcctgctg tgataatatt ttcctgacca
    19321 gctgggaggt taggattttc tttcttgata acttcaaatg gaatttcttc agttcttgtg
    19381 atgagttctg gtctggtttc aacattggca gccacttcat tttcatccag gcttcctgaa
    19441 tgagttgcag ctggtttgag gccttgaaga gcggctttta ggttggctac aagtgtgtca
    19501 agctcagctt gtttattacg gttgaggttg taatttagag ctgttttagc tgcgtcaagg
    19561 gcctcaagac tttctttact atatccttct aagtttgtag gaattttagc taattcttcg
    19621 cggagagcat tataattagc acggaagtag tctttgttgt ggtctgcaaa ggcagtcatg
    19681 agttcaaaga tttcctcttc cttgtattca gcgcttggtc tatctgccca gattgaaagc
    19741 atacttccga ctgttggaag atctacttca ggatatttgg tagaagctag ttgattgaat
    19801 ggtgtttttc cagtattctc aatagctttc ttgaggaaac caccaccatc ttctggtttt
    19861 tgaccaagaa tgtagtacca gtcaccgttg gtattcaaga atttatagcc tttgcttgct
    19921 aggtattgag gtgatgcgag gttatatccc caccagcctt tagaccagta agaaatcaag
    19981 acatctttgt caaactgaac atcgtccttg tcttcatagt agaagccatc gttgaaggcc
    20041 attggttgaa gccctctttc tttggccata gctgcgaggg tgttggcata ttcggcaaat
    20101 ttgccataga gttgatacca cttgaggtag taccagcctt gggcactagt cgcatcgttg
    20161 gcgtattcgt cagtaccaaa gttgaaaatc tttgttttac ctgcaaagaa gtccatgtat
    20221 ttaccgatga gggcttttac aaagttcatc gcttcttcgt ttttcaagtc catagttgtt
    20281 tttgaaactt tatcaaagtg ggcttgagga tttttaatac ctaatttttc catggcaacc
    20341 agcatagcat ccatgtgacc tggactgtta atagctggga tgagaccgat gtccttagat
    20401 ttagcgtatt caattagctc tgttacttct gcctgtgtta gtgcagtacc gtttggatcg
    20461 tcgtagtaag ctttagttcc ttcgataata gcttttttaa cgtcatcact agcataggtt
    20521 tttccgttgg cagtaatggt catatcatcg agtagaaagc gaagtccgtc atttcctaga
    20581 aggagatgga catcagaata tccgagctca ctggccttgt ctacgatgcg tttgagctgg
    20641 ttcagagtaa agtatttgcg tccagcatcg attgagatta ccttgttttt ggcaagtttt
    20701 tcaacctcac gtttagcttc ttcttctttt tgagcttcag gcgtgagggt caagttgttg
    20761 acagtttctt gaagtttagc aatggcttga tcaatcgtat cttgttgggc acggctaagg
    20821 ttgctatcga gagagcgaat agctttttca gcttctttta cggccgcgac gctttctgca
    20881 gtataacggt tcaggtcttt tggtacctcg ttaagtgttt gctctgcaga ttcataatca
    20941 gctgcgaagt attcagcgtt ggcatttgca aaatgacgca tgagtttgaa gaggcgtgat
    21001 ggtgaataac gtgcagatgg agtgtcagcc caagcagcta ccatactacc gatgattggg
    21061 atatcagctc cttctgtttt tggtacagaa gtgattggtg tgtttttaat accattgagc
    21121 ccctgatcga gattgtacca gccttggcca tcagcgtttc gtccaagaac gtagtaccaa
    21181 gcatcattgg tattaaggat ttggtgacct ttttcagcta gtagtttaga agaagcgaca
    21241 tcgtagcctc cccaaccacc agtccacata gaaacgatga tgtctttgtc aaaactacca
    21301 aagcttgtgt cgctattgta gtagataccg tcgttaaagg ccattggttt gagaccgtgc
    21361 gattttacaa tacgagcgag gtcattggcg taggcaataa atttttcata gccttttaca
    21421 gggtagcctt cgtttggata gtatttatca gcttgaagca cactccaacc tttagcatct
    21481 gtcgcatcat tggcatattc atcaagtccg atgttgaaga tttcagtctt tttcgcgaaa
    21541 taagcagcat acttgtcgat aagggctttt gtaaaagcga cagcttgttc gttgtcaaga
    21601 ttgacagtac gggctgattc cttcccaaaa tagctaaagt tagggttttg gattcccaat
    21661 tctttcatgg cattgagaat cgcatccatg tgaccaggac tatttactgt cggaatgaga
    21721 ccgatacctt tatctttggc atagttaatc agatctgtca tttgactttc tgttaagtga
    21781 ttgccgtttg gatcgttgta ataatcattt gtaccttttt caatggcgcg tttgacatcg
    21841 tcactggcat aggtcttgcc gttagctgtg atgctcatat cgtccaacat gaaacggagt
    21901 ccatcatttc cgactaatag gtgtaaatca gtgtagccat aatgtttcgc tttatcgatg
    21961 atttccttga gctgttctgg tgagaaatat ttacgtccag catcaataga aacaattttc
    22021 tttttcgcta gtttttcatt tacagttgca gcacgttcct ttcctgcctc tgtttccggt
    22081 ttgtcatcct ctgctttcgc ttcatctttt ttagctggtt tatccttgtc agtcttgtct
    22141 gtatttgact ctttagaatc aacctctttc gcttcttcct ttttagggct agcttcttct
    22201 gcctttttat tagcagtttc tttttcagca gaagttggag ttaccacttc tgctttatca
    22261 ctaggagttg aactaacttc ctcttgtggt ttttcttctg tttttggaag actagctacc
    22321 ttatcagtag ctggagtttc tgtttctaca gtttttggag cttctggttg aagctctgct
    22381 ttaggtgttt cctcagtccg attttcggat gattgagggg aatcagaaac cgtatggatg
    22441 gtcggttggt tttctgtagt agtaggagta actccatcgg ctgcaacagt ctgtgcttgg
    22501 aaggcaaatc caattagaac agaagctgct cctacagcgt atttacgaat agaaaaacgc
    22561 tgttgttttt catgtttcat tgcaaaacct cctgattgca ttgttatatt gatagcgatt
    22621 atataaatca acgcctttat tttatttctt atattaacga gagtcaagag gagatgacaa
    22681 aaaactataa taagtataaa aaaatataaa atttaaactt aagatttcag attggtcgga
    22741 aaaaatacgt atatatatct agtataattt ttggttctat ttctataaaa tattccacaa
    22801 attatagaat tttccaaaaa taggtaagcg ctaccttttt ggtgtggtat aataagcata
    22861 gaaaaagccc aagcgattag ctcaggcttt cttcttagtg atcacggtca catgagataa
    22921 atttaatctt gtagtaatca gatcgtttgt aagtttcact gtattctaaa acttggccag
    22981 ttgattcgag tttggtgatt ttagtttgta ggacagtagg gaattgttca tcgactccga
    23041 ggactgaagc cgcatgttct ggagttggaa agactatttc gttgatttct tcaaagtgtt
    23101 catcattcat gtgaatgtgg tagtctaact tgaaacgatt atagatagaa ctatagtatt
    23161 caaggtttgg ataatttgcg ttgatatatt gttctgggat gtaggatgta tggtagatat
    23221 aaacgacacc gtttgattcg cggatacgtt caatcttgta gtagaattga tcgccgcgta
    23281 gacccaattt ttccaagtaa acaagcttgt ttccgcgttc aattgaaaga acagttacct
    23341 tatcatcttt agcattgaag agttcaatat ctgaaaactc tacaagcttg tgtttgcgtg
    23401 cacgtgaaac gaaggttcct tttccttgtt ggcggacaat atagccatct ttggcaaggt
    23461 cgtttaaggc gcgaacaact gtgatagagc tgacatcgta cattgaaatg agttctgctt
    23521 cagtgtaaaa tttatctcca ctgctaaact gcccagagat gattttattt tttaattcgt
    23581 cttttatgta ttgatatttg ggaattgcca tatttttacc tctattttcc ttctccatag
    23641 gaaattttac cataaaagcg aaaaaaatag tagtcttttg aataaaaaat tagaataata
    23701 gtttgaaaaa ttaagcgata tagggattgc tcatatttta tcaatgtatt ctatacaaaa
    23761 ttattttagg tcccatattc cgaatttaaa gagcgtttga aatgggtcta aaaatgtttg
    23821 aaactaattg aatgtgtgta taatagtaaa aatgcagtaa atatcccgac aatttctatt
    23881 gacaattcaa acagattggt ttataattaa tataacaaca aatgaaagcg caaactttcg
    23941 cggtcggaag gtagttttat gacacgattt gagatacgag atgatttcta tctcgatgga
    24001 aaatcattta agattttatc tggtgccatt cattatttta gggttcctcc agaggattgg
    24061 tatcattcgc tctataactt gaaggctctt ggttttaata cggtagagac ttatgttgct
    24121 tggaatttac acgagcctcg tgaaggtgag tttcattttg aaggtgatct ggatttagag
    24181 aaatttctcc aaatagcgca ggatttgggt ctctacgcaa ttgtgcgtcc gtctccattt
    24241 atctgtgcgg aatgggaatt cggtggctta ccagcttggc tcttgaccaa gaacatgcga
    24301 attcgctcat ccgacccagt atatatcgag gcagttggtc gctactatga tcagttattg
    24361 ccaagactgg tgcctcgttt gttggacaat ggtggcaata ttctcatgat gcaggttgaa
    24421 aatgagtatg gttcttacgg agaagataag gcttacctga gagcgattcg acagctaatg
    24481 gaagagtgtg gcgtaacctg tcccctcttt acatcagatg gtccatggcg agctactctg
    24541 aaagctggaa ccttaattga agaggacctc tttgtaacag gaaactttgg ttctaaggca
    24601 ccttacaact tttcgcagat gcaggaattc tttgatgaac atggtaagaa atggccactc
    24661 atgtgtatgg agttctggga tggttggttc aatcgctgga aagaaccgat tatcacacgg
    24721 gatcctaagg aattggcaga tgcagttcga gaggttttgg aacaaggctc tatcaatctt
    24781 tacatgttcc acggtggtac aaactttggt ttcatgaatg gttgctcagc tcgaggaact
    24841 ttggacctgc cacaagttac gtcttatgat tacgatgccc ttctggatga agaaggaaat
    24901 ccaactgcta aatatcttgc agtcaagaag atgatggcaa cacatttttc agagtatccg
    24961 cagttggaac cactctacaa agagagtatg gagttggatg ctattccact agttgaaaaa
    25021 gtttctttgt ttgaaacctt agatagcttg tcaagtcctg tagaaagtct ctatcctcaa
    25081 aagatggagg agctgggaca aagttatggc tacctacttt atcgaacaga aacaaactgg
    25141 gatgcagaag aagaaagact tcgtatcatt gatggtcgag atagggccca gctgtatgtc
    25201 gatggtcagt gggttaaaac tcaatatcag acagagattg gggaagatat tttttatcaa
    25261 ggtaaaaaga aagggctatc taggttagat atcttgatag aaaatatggg gcgtgtcaac
    25321 tatgggcata agttcttagc ggatacgcaa cgtaagggaa ttcggacagg ggtctgtaag
    25381 gatctgcatt tcttactaaa ctggaaacac tatccactcc cactagacaa tcctgagaaa
    25441 attgattttt caaaaggatg gactcaagga caaccagcct tttacgctta tgactttaca
    25501 gtcgaagagc caaaagatac ttacctagac ttgtctgagt ttggtaaggg agttgccttt
    25561 gtcaatgggc agaatctagg acgtttttgg aacgttggcc caactctctc actttatatc
    25621 cctcatagct atctcaagga aggtgccaac cgtatcatta tctttgaaac agaaggtcaa
    25681 tataaagaag agattcattt aactcgtaaa cctacactaa aacatataaa gggggaaaac
    25741 ttatgacaat tgtaggatgc cgtattgatg gacgtttgat ccacggacaa gtagccaatc
    25801 tttgggctgg aaaactaaat gtttcacgca ttatggttgt agacgacgaa gttgtcaaca
    25861 acgatattga aaagagtggt ttgaaacttg cgacaccacc aggtgtgaaa ttgagtattt
    25921 tgccagttga gaaagctgca gccaatattc ttgctggtaa atacgatagc caacgtctct
    25981 ttatcgtggc tcgtaaacca gaccgcttcc ttggtttggt agaagcaggt gtaccacttg
    26041 aaacccttaa tgttgggaat atgtctcaaa caccagaaac tcgttctatt acacgttcta
    26101 tcaacgtagt agacaaggat gtggaagact tccacaaact ggcagaaaaa ggtgttaaac
    26161 ttactgctca gatggttcca aatgatccaa tttcagactt tttgagctta ttaaaatagg
    26221 aaaaaaattt ttaggaggtc attgttatga tacaatggtg gcaaatttta cttctcactt
    26281 tgtactcagc ttatcaaatc tgtgatgagt tgacgatcgt ttcatctgca ggttcccctg
    26341 tatttgctgg tttcattact ggtttaatca tgggagatgt gactactggt ttacttatcg
    26401 gtggtaactt gcaactgttc gttcttgggg ttggtacctt cggtggtgct tctcgtatcg
    26461 acgcaacttc tggtgcggtt cttgcgacag ccttctctgt ttcacaagga attgatgcac
    26521 cgcttgcaat tactacaatc gctgtaccag tagcagctct cttgacttac ttcgacgttc
    26581 ttggtcgtat gactactacc ttcttcgctc accgtgtgga tgctgcaatc gaacgctttg
    26641 actataaagg tattgaacgc aactacttgc ttggtgcgat tccgtgggct ctatctcgtg
    26701 cccttccagt cttctttgcc cttgcttttg gtggtgcctt tgtacaatca gtagtagact
    26761 tcgttgaagc ctacaaatgg gttgcagatg gcttgacact tgcaggacgt atgcttccag
    26821 gtcttggatt tgcaatcttg cttcgttacc ttccagttaa acgtaacctt cactaccttg
    26881 ctatgggatt tggtttgaca gctatgttga ctgttcttta ctcatatgta acaggtcttg
    26941 gtggcgctgt tgctggtatc gtaggtactc ttcctgctga agttgctgaa aaaattggtt
    27001 tcgtgaacaa cttcaaaggt ttgtctatga ttggtatttc tatcgtaggt attttccttg
    27061 cagtgcttca cttcaaaaat agccaaaaag tagctgtagc agcaccttct acaccatcag
    27121 aaagtgggga aatcgaagat gacgaattct aattacaaac ttacaaaaga agattttaat
    27181 caaatcaaca aacgtagctt gtttactttc caattaggtt ggaactacga acgtatgcaa
    27241 gcttctggtt acctttacat gatcttgcct cagttgcgta aaatgtatgg tgatggaact
    27301 cctgaattga aagaaatgat gaaagttcat actcaattct tcaatacttc accattcttc
    27361 cataccatta tcgctggttt tgaccttgcc atggaagaaa aagatggtgt aggttcaaaa
    27421 gacgctgtta acggtatcaa gacaggtttg atgggaccat tcgctcctct tggggataca
    27481 atctttggtt cacttgtacc tgctatcatg gggtcagtcg cagcaactat ggctatcgct
    27541 ggccaacctt gggggatctt cctttggatt gcagttgcag tagcgtatga catcttccgt
    27601 tggaaacagt tggaatttgc ttacaaagaa ggggttaacc ttatcaacaa catgcaaagt
    27661 accttgacag ctttgattga cgctgcatct gtacttggtg tcttcatgat gggtgctctt
    27721 gtagcaacag tgattaactt tgaaatttct tacaagttgc caatcggtga aaagatgatt
    27781 gatttccaag acatcttgaa ccaaatcttc ccacgtttgc ttccagcaat ctttactgcc
    27841 tttatcttct ggttgcttgg taaaaaaggt atgaactcta ctaaagctat cggtattatt
    27901 atcgtacttg ctttggctct ttctgccctt ggtcactttg cacttggaat gtaattcctt
    27961 atgactaaat cattaatttt ggtgagccat ggtcgcttct gtgaggagct tagaggtagc
    28021 acagaaatga ttatgggccc acaagacaac atttacacag tagctcttct tccagaagat
    28081 ggcccagaag aatttactgc taaatttgaa gctgttattg aaggattgga tgatttccta
    28141 gtctttgcgg atcttctcgg tgggacacct tgtaatgtgg tgagtcgctt gatcatggaa
    28201 ggtcgtgata ttgaccttta cgcagggatg aatcttccaa tggtgattga atttatcaat
    28261 gcgagcctta caggcgcaga tgcggactac aagagccgtg ctgcagaaag cattgtgaaa
    28321 gttaatgacc tgttagcggg cttcgatgat gacgaagatg aataatactc ttcgaaaatc
    28381 tcttcaaact acgtcaacgt c
//
LOCUS       DAVBCG010000015        27493 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBCG010000015 DAVBCG010000000
VERSION     DAVBCG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27493
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.015"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(96..392)
                     /locus_tag="VQ495_000530"
     CDS             complement(96..392)
                     /locus_tag="VQ495_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2530525.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(545..1000)
                     /locus_tag="VQ495_000531"
                     /pseudo
     CDS             complement(545..1000)
                     /locus_tag="VQ495_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            1134..1961
                     /gene="xth"
                     /locus_tag="VQ495_000532"
     CDS             1134..1961
                     /gene="xth"
                     /locus_tag="VQ495_000532"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2530526.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            1973..2116
                     /locus_tag="VQ495_000533"
     CDS             1973..2116
                     /locus_tag="VQ495_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2530527.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            2116..2355
                     /locus_tag="VQ495_000534"
     CDS             2116..2355
                     /locus_tag="VQ495_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2530528.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      2739..2834
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            3234..3815
                     /locus_tag="VQ495_000535"
     CDS             3234..3815
                     /locus_tag="VQ495_000535"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2530529.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            3815..5077
                     /locus_tag="VQ495_000536"
     CDS             3815..5077
                     /locus_tag="VQ495_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2530530.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(5164..6030)
                     /locus_tag="VQ495_000537"
     CDS             complement(5164..6030)
                     /locus_tag="VQ495_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2530531.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(6017..6787)
                     /locus_tag="VQ495_000538"
     CDS             complement(6017..6787)
                     /locus_tag="VQ495_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2530532.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(6813..7667)
                     /locus_tag="VQ495_000539"
     CDS             complement(6813..7667)
                     /locus_tag="VQ495_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2530533.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(7824..8228)
                     /locus_tag="VQ495_000540"
     CDS             complement(7824..8228)
                     /locus_tag="VQ495_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2530534.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(8298..9779)
                     /locus_tag="VQ495_000541"
     CDS             complement(8298..9779)
                     /locus_tag="VQ495_000541"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2530535.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(9798..10976)
                     /locus_tag="VQ495_000542"
     CDS             complement(9798..10976)
                     /locus_tag="VQ495_000542"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2530536.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            11141..12148
                     /gene="galR"
                     /locus_tag="VQ495_000543"
     CDS             11141..12148
                     /gene="galR"
                     /locus_tag="VQ495_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2530537.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGFEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(12194..13231)
                     /locus_tag="VQ495_000544"
     CDS             complement(12194..13231)
                     /locus_tag="VQ495_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2530538.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEVDDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATVVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(13232..13585)
                     /locus_tag="VQ495_000545"
     CDS             complement(13232..13585)
                     /locus_tag="VQ495_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2530539.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(13829..14719)
                     /locus_tag="VQ495_000546"
     CDS             complement(13829..14719)
                     /locus_tag="VQ495_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2530540.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            14852..15388
                     /locus_tag="VQ495_000547"
     CDS             14852..15388
                     /locus_tag="VQ495_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2530541.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(15555..16361)
                     /locus_tag="VQ495_000548"
     CDS             complement(15555..16361)
                     /locus_tag="VQ495_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2530542.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(16706..18226)
                     /locus_tag="VQ495_000549"
     CDS             complement(16706..18226)
                     /locus_tag="VQ495_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2530543.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(18219..18947)
                     /locus_tag="VQ495_000550"
     CDS             complement(18219..18947)
                     /locus_tag="VQ495_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530544.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(18962..19726)
                     /locus_tag="VQ495_000551"
     CDS             complement(18962..19726)
                     /locus_tag="VQ495_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2530545.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFREDENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(19730..20173)
                     /locus_tag="VQ495_000552"
     CDS             complement(19730..20173)
                     /locus_tag="VQ495_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2530546.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(20855..21151)
                     /locus_tag="VQ495_000553"
     CDS             complement(20855..21151)
                     /locus_tag="VQ495_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2530547.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(21173..22237)
                     /gene="pepA"
                     /locus_tag="VQ495_000554"
     CDS             complement(21173..22237)
                     /gene="pepA"
                     /locus_tag="VQ495_000554"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2530548.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            22638..23339
                     /gene="galE"
                     /locus_tag="VQ495_000555"
     CDS             22638..23339
                     /gene="galE"
                     /locus_tag="VQ495_000555"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2530549.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            23762..24721
                     /locus_tag="VQ495_000556"
     CDS             23762..24721
                     /locus_tag="VQ495_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2530550.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLGFILLLWRGGRKDAD"
     gene            24711..25667
                     /locus_tag="VQ495_000557"
     CDS             24711..25667
                     /locus_tag="VQ495_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2530551.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            25664..26416
                     /locus_tag="VQ495_000558"
     CDS             25664..26416
                     /locus_tag="VQ495_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530552.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            26512..27477
                     /locus_tag="VQ495_000559"
     CDS             26512..27477
                     /locus_tag="VQ495_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2530553.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
BASE COUNT         8114 a         6337 c         4897 g         8145 t
ORIGIN      
        1 agatagaact gacgaagtca gtaacctaca tacggcaagg cgacgttgac gcggtttgaa
       61 gagattttcg aagagtacaa gaccccaggt ttttcttatt cataagttac gactgtaaca
      121 gcacccttgt catattcagc aataaagata ttggctacat tgtcatgccc ttgtttgctg
      181 aggttatcaa gcaaccactc ttcgctacga ccaatcgatt ccaagacgtc tacttgaatc
      241 acaccgtcag tcacaactgg atacttagga ttttcatctc ccatttgcac aacgatgagt
      301 tgcccatttt gctcttgcac agctcgtttg acttgcttca tctggaaaat cccttggcta
      361 cgaagtttga gggctacatc cgatgcagac aaaccaactg aacgacaggc ttatgggtca
      421 atattcccat ttttgataag gagagttggc ttaccatcaa tcaaacgttt cacaaagtga
      481 acattgttgt tgagctattt gagagtcaag atcagtttga ttaaaatttc gatataatta
      541 agtgtcattt gttcacatcc accagttcaa ttgcgtctgt cttatacaat tcgactttct
      601 ctaacaggta cttgtctggc tcacttccgt ttagggcacg ataatagcga tctcccacct
      661 tgataagtgc gccatctgtg tttgtggaag tattaacatg gacttctgac ttgtctactc
      721 ccaaatcttt ggaaacaacc tcgatgaaat gaagtgaagt ttgaaattta ttgttagaaa
      781 cttgattgtt ttgataagtc gtgatgctga ccaaaagcat agctaaataa ggccagagct
      841 gaaatcatga ccaactcacg aaatttggtt ccttttttat catggtatgc cttgaaagca
      901 aaaaaactgt cacagctaat agaacaatcc caaagccaac catgatacca ttttgctgac
      961 cgatttggct gagtacatag tcataagagt agaatttcat gtattttcac tccctttcat
     1021 aaaatcattc ttaattataa acgaaagaga gtaatttttc aaaccatacg ttggggaaat
     1081 agatcttttt ttggtataat aaaatctata atctgaatga aaaaggtaac tttatgaaac
     1141 ttatctcatg gaatattgat tccctaaacg ctgccctaac tagtgactca gctcgtgcca
     1201 aattgtccca agaagtccta caaaccttgg tcgctgaaaa tgctgatatc attgctatcc
     1261 aagaaaccaa gctttctgcc aaaggaccta caaagaaaca cgtggaaatt ttagaagaac
     1321 tcttcccagg ctacgaaaac acgtggcgct cttcccaaga gcctgcccgt aaaggctatg
     1381 ctggaaccat gttcctttat aagaaagaac ttacacccac tatcagcttc ccagaaatcg
     1441 gtgccccttc taccatggac ttggaaggcc gtatcatcac tctagaattt gatgcatttt
     1501 tcgtaaccca agtttacact ccaaacgctg gtgacggtct caaacgcttg gaagaacgcc
     1561 aagtctggga tgtcaaatat gcggagtatt tggctgaact agacaaagaa aaaccagtcc
     1621 ttgcgaccgg tgactacaac gtagcccaca atgaaatcga ccttgcaaat cctgctagca
     1681 accgccgttc acctggattt actgacgagg aacgtgctgg atttaccaac ctattggcaa
     1741 ctggatttac tgataccttc cgccatgttc atggcgatgt tcctgaacgc tacacttggt
     1801 gggcacaacg cagcaaaact tctaaaatca acaatacagg ctggagaatc gactactggc
     1861 tcacaagtaa ccgtatcgct gacaaggtca ctaaatctga tatgattgac tcaggtgctc
     1921 gccaagacca tacaccgatt gtcttggaaa ttgacttgta aggattttct atatggacta
     1981 caatgcggtc attcccgagt ttcttgtatc gaatatcgaa cagtcccggt ccttctactg
     2041 tggtttactg ggttttagga ttgaatacca acgttcagaa gaaaactttc tctttctctt
     2101 gaagagtgtc aactaatgtt agaggaggga acaaaggatc aactagctga actgacctac
     2161 ccctttggtc gtggagtcaa tctctccttt ggcataaagg atgttccaaa actctatcaa
     2221 aaagtgatgg aggcaaacta tcctatttat cgtcccttga ccaaaagaaa gtttcgtgtc
     2281 agtgatccct atatctatcc tcataaattt gcagttttgg atccagatgg ctatttttta
     2341 agatttagcg agtagaataa taaaaattgg gaaaagtctt taaaagttag aaaaaggcat
     2401 attgtcaggt gtctaaaaac ttgatagtat gccttttatg atggaaaaat cttctaatac
     2461 tcttcgaaaa tctcttcaag ccacgtcaac gtcgccttac cgtaggtata taatactgac
     2521 tctgtcaatt ctatccacaa cctcaaaaca gtgttttgag gttgtggata gcttcctagt
     2581 ttgctctttg attttcattt agtataaatg ttagcttaca atggagtttt tgttcaccct
     2641 ctcttctgtt tgttcgtttt ttaaaccaaa acactaacaa aatagttgat ttctaaagtt
     2701 atttagtgta aaataaaaac attggctcaa gcctatttaa aattgaatat cgttttactt
     2761 gtttatgtcg tgaattggca cgacgtttct acaaggtgcc ggaacaccta acaataagta
     2821 agtcagcagt gagatatggc cgtttttgcc atgcctattt gggggactta cttagagatt
     2881 aagtaggtct tttttgaata tttttaatat ttcccttaat tagtgcggac gggaggtgaa
     2941 attatcctgt gaataatttc aggaatccag ggaatttcat ccctaatttt tgagccccta
     3001 gctctttcat ttctaggctt aggctaaaat agttccccga actattttac tctcaaaaat
     3061 cccgtcatag acaataacaa gattattgtc tatgatacca ctacaacggg aaatatttga
     3121 taagactcgc ttcgctcgta atagttttat caaatatata gcattagcaa aagggatcac
     3181 tatagaaaca tttaaacaac aagtaaatta acttttagac tttaggaggt cttatgaaat
     3241 tattagaaga gcgcatcctc aaggatgggc atatcttggg tgataacatc ctcaaggtag
     3301 attccttttt aacccaccaa gttgacttta gcttgatgcg agagattggt aaggtttttg
     3361 cggaaaaatt tgctgctgcc ggcattacca aggtcgtaac cattgaagcg tcgggtattg
     3421 ccccagccgt ttttacagct gaagccttaa acgttcccat gattttcgcc aaaaaagcta
     3481 agaacatcac catgaacgaa ggcatcttaa ctgctcaagt ctactccttt accaagcagg
     3541 tgaccagcac cgtttctatc gctggaaaat tcctctcacc agaggacaag gttttgatta
     3601 tcgacgattt ccttgctaat ggccaagctg ctaaaggctt gattcaaatc atcgaacagg
     3661 ccggtgccac agtccaagct atcggtatcg tgattgagaa atccttccaa gatggccgtg
     3721 atttgcttga aaaagcaggc taccctgtcc tatcacttgc tcgtttggat cgttttgaaa
     3781 atggtcaggt cgtatttaag gaggcagatc tctaatgcaa actcaagaaa aacactcgca
     3841 agcagccgtt cttggcttgc agcacttact agccatgtac tcaggatcta tcctggttcc
     3901 catcatgatt gcgacagccc ttggctattc agctgagcag ttgacctacc tgatttctac
     3961 agatatcttc atgtgtgggg tggcaacctt cctccaactc caactcaaca aatactttgg
     4021 gattggactc ccagtcgttc ttggagttgc attccagtcg gtcgctccct tgattatgat
     4081 tgggcaaagc catggtagtg gcgctatgtt tggtgccctt atcgcatctg ggatttacgt
     4141 ggttcttgtt tcaggcatct tctcaaaagt ggccaatctc ttcccctcta tcgtaacagg
     4201 atctgttatt accacgattg gtttaacctt gatccctgtc gctattggaa atatgggaaa
     4261 taacgttcca gagccaactg gtcaaagtct cttgctttca gctattactg ttctgattat
     4321 cctcttgatc aacatcttta ccaaaggatt tatcaagtct atctctattt tgattggtct
     4381 ggttgttgga actgccattg ctgctactat gggcttggtg gacttctctc ctgttgcggt
     4441 agctccactt gtccatgtcc caactccact ctactttggg atgccaacct ttgaaatctc
     4501 atctattgtc atgatgtgta tcatcgcaac ggtgtctatg gttgagtcaa ctggtgttta
     4561 tctagccttg tctgatatca caaaagatcc aatcgacagc acgcgccttc gcaacggtta
     4621 ccgcgcagaa ggtttggccg tacttctcgg aggaatcttt aacaccttcc cttacaccgg
     4681 attttcacaa aacgttggtt tggttaaatt gtcaggcatc aaaaaacgcc tgccaatcta
     4741 ctacgcagct ggtttcctgg ttctccttgg actgcttcct aagtttggtg cccttgccca
     4801 aatcattcca agctccgtcc tcggcggtgc catgctggtg atgtttggtt ttgtatctat
     4861 tcaagggatg caaatcctag cccgagttga ctttgctaac aatgaacaca acttccttat
     4921 cgcagctgtt tcaatcgctg caggtgtcgg tctcaacaac agtaatctct ttgtcagcat
     4981 gccgacagcc ttccaaatgt tcttctcaaa cggaatcgtc gtagccagcc tactcgccat
     5041 tgtcctcaat gccgtattaa atcataaaaa gaaataagaa aaagaggtgc gagcctcttt
     5101 tttgtttatc acaaattgat tataaataca atcaatccca attcccatct aattgaattg
     5161 gacctatttc aaattcttta aaatattatg ttccaaatcg ttaatattat ataagaatgg
     5221 taaaatatca aatgtttctc gtaaattatt cttagcctta taccaacctt gtccatcagt
     5281 aatccacata aactcaacat taggaatagc tttaatttct tcagcaatca ttttgtaaga
     5341 tctagccgtc tcatttaatt ttgaaccact tcctgaataa aaattaacct caatcagata
     5401 aagcacttgt tcatttttta tgacaaaatc aaaacgtttc acagtattcc catcattcgt
     5461 aatagcagat aaatcgacag aaaaaatttc ctctatttca ttttgttcaa tctctttaaa
     5521 taaattctct cctaaaatat acccttcagc ttcaagataa ctttgaacaa tattttccat
     5581 cgcatctcca gtccgatttt tgcgcccatt agtatccata ccaacctcaa cacctgtaac
     5641 gtagtcaact aaatttgata ctaaatgatt ttgcaataaa tcaaatatac ctgatttttc
     5701 caaaaacata gtatattctt caatactata gtttcgttta ctaaaatcaa aatagaaatc
     5761 ttttatcgga tccttaacaa taatcgtatc tctcaaacgt ttagcaataa gtagaggcac
     5821 aactttcaaa atttctggat aattttgata taagtctaaa aaatcctctt gtatattttt
     5881 actaccaata agactattca taatatttag agcaactttt atactagata catttttata
     5941 aaccttggga aaatctgtat aataagtcca gtcagctact gtgtcggtca tcgttgacaa
     6001 ccactcatca aaatttctag tttgtttcat tttctgcctc caacctcttc cttgcaattt
     6061 ttaaatattc tttttcagca tcgataccta taaatcttct acctaaccgc ttcgcaacaa
     6121 cacccgtagt gccactacca acaaatgggt ctagaatata gtcaccctct ttagtagagg
     6181 ctaaaataat gcgttctaac aaatactctg gtttttgagt tggatgtttc ccagcccatt
     6241 tttcaacttt ctttgttaaa gaacccgtcc agacatcttt catttgtttt ccatcattca
     6301 attctttcat taaatcataa ttgtagtaat gacgagcttt tttatcattt tttctggccc
     6361 ataaaatggt ttcagtagaa tgggtaaaat aacgacaaga taaattgggg gcagggtttg
     6421 ttttctgcca agtaatatta ttcagaattt taaaaccttc ttgttctaat gccattccaa
     6481 ctgagtatat gttgtgcaaa ctacctgaaa tccataccgt cccattaggc ttcagaactt
     6541 cttttgctag gcgaatccat ttacgattaa actcatgttt ttcttcgaat gaagaaattt
     6601 tatcccaatc tcctttatca acagaaacaa cttgtccccc agaattagat attccaccat
     6661 tacttaaaaa ataaggtgga tcagcaaaaa tcatatccat actttctggt ttcatttttg
     6721 ataagaactt gaacgtatct gaatgaacaa gaatcatctt atttttattg tagtatggtt
     6781 ttgtcataag aacacctcca tacttatatt cgttattttt cataatttgt gactataatt
     6841 tcagaaattt ttcctcgact tgaagatttt gctccattag ttcgggtagc ttcaacataa
     6901 tgtatattaa aatccttata caactcctct actaaagcac tagaagaatt tgataacata
     6961 acataagctc ctgtatcact caatctctta aaggcatctc ttaatcttac ttgatctgca
     7021 aaagagaatc cctcatgagt ataagacgta aatgcactcg tctcagacaa tggaatatat
     7081 ggagggtcaa aatacacaaa atctcctgtt cgaacatcta caattgcctt ttcaaaatct
     7141 cccactttaa tttctagttg attgttattt agataaactg aaatagcaga tatcaattcc
     7201 tcatcaacaa ttttaggatt cttataacgt ccatatggaa cattaaattg gttcttagaa
     7261 ttcacacgat atagaccatt aaagttcact ctcaacatat atagaatacg tgcagctctt
     7321 tgtacttcgg acatcatatc tattctttca tcacgatctg cagaacgtaa atctaaataa
     7381 tattcttttg aattatattc ctgatgaact ttcaaaattt caatcaattc ttgaggattg
     7441 tccttaattt gttgatagca atttattagt tcagcgttaa aatcattaat aactgcatct
     7501 ttaggagcca aatcaaaaaa taaagctcca cctccaacaa aaggttcgaa atacctgtta
     7561 taggttttag gcattaattc tctaataaca ggcaataatt gtcttttacc acctgtccat
     7621 ttcgtgaacg gttgtaaagt aactttcttt atttctttta tcttcatgct tttaattata
     7681 ccatagaaat taaaacacaa ctcttgtatt ttctaccaca ttactaaatt ttctatttta
     7741 gaattaaatc atatcaatat aaaaaaatga gtaaggcaaa tcttataaat tccaaaattc
     7801 acctcactct attaaattac tctttatatc cttacccgtc tttcctcaga cctttggcct
     7861 gtgacaaaca tagtaaaggt tgctttgcag acatttctgc cctcttgatt ggtgatatcc
     7921 acatccatca cacaggttgt acgaccttga tggacacatt ctcctttaat ggtcaataca
     7981 tcgtcgagtt ttcctgcctt aaggtagttg atagaggatt ggagtgtcac cccatcaagt
     8041 cccagcgaga taaccaccaa accactgatt tggtcacata gtgtgaatag atagccacca
     8101 tgggcattgc catagtagtt gagcgacgag ttcactactt tggtcgtcac cacaacgtga
     8161 ccatctctca ttttttcaat ttcgtaattt tcaaaggcag atatagcgtc aaaatgaaag
     8221 tctttcatcg tttcctcctg atatttcaaa tgacactatg atactacttt ttataggact
     8281 gtgcaaggag aaagctccta gtctagtaaa attccgacat gttccacaaa gcgcataaag
     8341 gctgtctgcc cttgttctgt ctgcttgtag actcctgcat cctcaagtac acgcgcaaag
     8401 atagcaccca cagagtcctt gacgattgca agggcttttt ctttatccgt taggtctgga
     8461 tgttgggatt tgagttggtc tgcccactcc tgatgataat cggcaactgt aacagcttct
     8521 cctacaagat agctagcgac ttgctctact tcttctttca gacgtggtgg caagattgcc
     8581 aagcccatga cctcaatcaa gccgatattt tccttcttga tatgttggac atccttgtgg
     8641 ggatgataga tgccatcagg atgctctgct gaagtctgat tgtctcgcaa gaccaagtcc
     8701 aactcaaact gtccatcacg cttacgcgct attggtgtga tggtgtgatg cggtgtcccg
     8761 tctgtctctg ctaaaatctg cacactagaa tctgaatact gacgccattc ctggaaaatc
     8821 ttatcagcta agttaatcaa atcctctttg gaatccgaag tcaagcgtag gacagacatt
     8881 ggccacttga taattccagc cttgacctgc tcaaaaccag caaatcggaa ggtcttttgc
     8941 aagggagcca attccatagg aaatacgtga cggcctccct gataatgatc atgagttaga
     9001 atagagcccc ccacaatcgg caggtcggca ttagatccag caaaatatcc tggaaactgc
     9061 tctacgatag ccaacagacg ttcaaaactc tgacgactaa tggccatggg acgatgctgg
     9121 ccatctaaaa agatacaatg ctcattaaag taagcatagg gcgaatactg gaaaccccat
     9181 tcctgaccaa ccatttcaaa acggataatc cggtggttgc tgcgagctgg gtggttgacc
     9241 cgaccatggt aaccctcatt ctctaaacaa agttgacact gaggataatt actagcttgc
     9301 accaacttgg ctaccgcaat ctctttcgga tccttttcag gcttagagag attgatggta
     9361 atttcaagtt ctccataatc agatggaaca cgataagcga tatttttagc aatggccttg
     9421 agtttgatgt agtcattttt ctgactgagt tgataaaaat cctctatcgc ttgctcagga
     9481 gagtaggcat aggcttccca gaagtcacga ttgacctgac tcggacaagg agtcaccaag
     9541 tccatcaatt cagtaccaag gatttcacgc gcagtctgac tatcctcaat cgtctctaat
     9601 cgaacggctt cctcaactag ctggtccttg aggtcaatca atttatccag attggtctca
     9661 acttccaaaa caccttctcc cactcgtgcc aaaacacgat tggtcaggta gattcgatcc
     9721 atttcctcaa atgaactttc agaaatgaca tgtgttacaa atttatctac taaggtcact
     9781 atagagcctc cttttgacta gtcaaggacg cgagtgccac ctgcaacttc agcgatatag
     9841 aagcttggag cgtatccaac tacttcctcg tagtgtttgc ctacagcttc cttaaaggcc
     9901 tcaacagtat ctttttgaac caaggcaatg gcacagccac caaaaccagc ccctgtcata
     9961 cgagcaccga gaactccttc ttgtgcccaa gctgtgtgaa caagagtatc caattccaaa
    10021 ccagttactt cataatcatg ctccagagaa acgtgtgacg cattcatcaa acgaccaaat
    10081 gtttccaaat cacctgcttg aagggctgct tgagctttaa gggtacgttg gttttcaaac
    10141 acagcatggc gagcacgttt caaacgattt tcatctttaa tcagatagct atattggtca
    10201 acggcccact cgtccaattc acccagggtc tgaatatcca aggcaacttg caattcttcc
    10261 actgcttttt cacactcagc acgacgttca ttgtatttag agtccgccaa ttcacggcgt
    10321 ttgttggtgt tcatgataac aacgacattg tccttcaaat caagtggcac caagtcgtat
    10381 tctaaagtat tggtatctaa gtaaatagca cgttggtcag cccccatacc aatagcaaat
    10441 tggtccatga taccagagtt gactccgata aagttatttt ctgtttgttt tccgatttta
    10501 accaaatcga gacgctctag ttttaaatca aagagatgct ctgccacgac tcctgtcaag
    10561 agctccaagg atgctgaaga agacaaacca gcaccatttg ggatattccc ataaacataa
    10621 aaatcaaaac ctttgtcaat cacgtgccca gcttcttgca agaaatgaag gacacctttt
    10681 ggataattgg tccagttgtg ctctttttca aacttgaggt cagcgagagg cacttcgata
    10741 atgcccttgt cctcaaagtt agctgagtag aaacgcaaga cttggtcgtc acgcttacga
    10801 gctgcaccgt aagttcccaa ggaaatagca gcaggaaaaa cgtgcccacc gttgtagtct
    10861 gtgtgttcac caatcaaatt aatgcggcct ggtgaaaaga aggtttggtc tgcttcttga
    10921 ccaaaaacag caagaaagtc tttgcgaagg gcttcagtag taagatgttg tgccatatga
    10981 attctccttt gactgtccgt taagccacct taacgtataa tcgttttctt tctattgtat
    11041 ccaaggaatt ttccaagatc aatccttttt actaaaattt tagtaaaata tcagtaaaaa
    11101 gaagaaaaac atgatatact aacctaatca aggaggattt atggctacct taaaagacat
    11161 tgcacagcta gcctctgtct ctatcgcgac cgtatcccgc gtcctcaatc gcgaccagag
    11221 cctatctgtt acagaagaaa ccagacaccg tattttaacc gttgctgaag agctgggcta
    11281 caccaagcac ctcaagacag gcgagtccca caaacccaag caaaagattg ccattatcca
    11341 atgggtcagc gaacaagggg agctggacga cctctactac taccagattc gcctaggaat
    11401 agaaaaaaga gcccaagagt tggactatga tatcttgcgc tattttaatg accatccttt
    11461 taccttaagc gaggaagtga ttgggattct ctgcattgga aaatttagcc gagctcagat
    11521 ttctgccttt gaagaatacc aaaaacccct ggtctttcta gacagtgata cactttctct
    11581 ggggcatact tgtattatta cggattttta cactgccatg aaacaggttg tcgattattt
    11641 cctcagccaa ggaatggacc gtatcgggat tctaacaggc tttgaagaaa caactgacca
    11701 agaagaaatc attcaggata agcggctgga aaatttcaga aactacagtc aagcaaaagg
    11761 aatctatcat gatgaactgg tctttcaagg aaactttact gcccagtctg gctatgactt
    11821 gatgaaggaa gccattcaga acttgggaga ccaactccca ccagcatttt tcgcagccag
    11881 cgatagttta gctatcggtg cccttcgtgc cctccaagaa gctggaatca gcctaccaga
    11941 ccgtgtcagc ctcatttcct ttaacgacac cagcctgacc aagcaggttt atcctcccct
    12001 ctccagcatc acagtttata ctgaagaaat ggggcgagca ggtatggata ttcttaacaa
    12061 ggaagtcctc cacgatcgca aaattcctag cctgaccatg ctgggaacca gactgacatt
    12121 aagagaaagt accctaaatc aagaatagga taacataaaa aacgaataga gttctaaaac
    12181 tcctattcgt tttttattcg attacaatca tagacttaat ggtcttacgt tcatccatat
    12241 ctttgtaggc ttggtcgata tcttccagtt tataacttga agtaaagacg cgacctggat
    12301 tgatatcacc atcaaggacg gcttttagta aaaattgctt atcgtatgtt gtagcagaag
    12361 ctgccccacc tgctacagag atattttgca taaatgtcga accaagagca cgattattat
    12421 agtgtgggac tcctacaaag cccatacgcc ctccattatg aagaacacct agcgcctgtt
    12481 ctatagcagc ctccgtacca acacattcaa gtgctgcgtc tgctcctcca ccgaggattt
    12541 cacgcacctt ggtaattcct tcttgaccac gttctgcaac aacaactgtc gcacctgact
    12601 ccatagccat cttttgacgg tcttcatgac ggctcataag gataatttgt gatgctccac
    12661 gcatcttagc cgcgatgaca gcacattgac caacagcccc atcaccgata acaacaacct
    12721 tgtccccttt ttgaacattt gcaacacgcg ccgcatgata gcctgtcggc atgacatctg
    12781 caagagtcaa aagggacttg agcatccctt ctgtatagtc agaaggttga ccagggattt
    12841 taaccagcgc ccagtttgca tagtggaagc gaatatattc tgcctgaaaa tcacccccca
    12901 aattattgcc aatatgattg tcgcaagaac cgtcaaatcc agcaagacag gcatcacact
    12961 caccacatcc atgtgtaaaa gggacaatca caaaatcacc tggtttcacc gtcgtaatgg
    13021 cttccccagc ttcttcaaca atcccaatcg cttcgtgtcc actatttttg tgtccagctt
    13081 tcgtttctgg attacggtac ctccataaat ctgaaccaca aacgcacgca cgaaccacac
    13141 gaataatcac atcatccact tctattattt gcggacgttc aatgctagca agtccaacct
    13201 gacctgcctt tgtatatact gctgatttca tttaaaattt tccttcctta taaagtttaa
    13261 ttttgagatt taaacgattt aaagctgtct gtagctgaga caagcgctcc tctaattttt
    13321 gcttttcctc ttctaaaata ccaagcctct cctctctcgt ttcatctccc ttttggtaga
    13381 gcgacatata gtcaactaaa ctatctacag atacacccgc cgaacgaaaa cacttaataa
    13441 attccagcgc ttcgatatcc tgatcttgaa aatcacgaat cccagtagca gtacgagtaa
    13501 tcggtggcac aagaccaacc cgttcataat accgaatcgt atccgctgaa attcccaaca
    13561 aatcactggc agatttaata ttcacacgct aacctcctct catttcattc actgaattca
    13621 cgctattcta actcacttat cttattatat aactttgagt tgactccaag tcaagcattt
    13681 tgtatctaat cgaaaaattt ttatcattaa caatcatata tccggagttt atacaatgct
    13741 cttctcttac caatttttga aatatttaaa taataaagaa attactctat tccaaataag
    13801 atagtaccca agtatttttc acttttttct aatgttgatg ctcataactc cgttccaagt
    13861 cacacacctt tcgcttatgg gtttggtgag tttctaggtc agcatcaatt tcaatggtaa
    13921 tattttgaaa accacaatct tttaggaaaa ttcgaataga ctctttacaa gtttccatat
    13981 gttccatttc ttttagacaa acatggacaa tggcattttt ttccaaagca tccatagtcc
    14041 agagattaag ctgattaagg ctggccacat tgtccaatcg ctccaggcca ctctttactt
    14101 gcttgatatc aagaccttct ggcacagcat ccaagaaaat cttgagtgta gaccaaaaac
    14161 gtggaagggc ttttgaaaga ataaagaaag aaatgacaag ggacaaaaga ggatctagga
    14221 tataccagtc cgtaaatcga agaacaatcg ccatcaggat aacagctacc caccctagcg
    14281 tatcttccag aaaatgcaga ctcagaatag actcattctt tgtctttccc ttaccaacca
    14341 ccagactcgc taacagattg atagtaatcg caataattcc taaccagaga atcccctcat
    14401 cattgactgg ttgcggatgc aaaatcttcg tgacattttc caaaatgact agaacagagc
    14461 ccgttacgag aatcacagct gttaccaagg ctcctagcag gctaaaccgc ttatagccca
    14521 aggtgtactg attgtcttct tcacgattgg agattgtttc tagaaaagct gatattccaa
    14581 ttgcaatcgc atctcccaag tcatgcacag agtcagcaag aacagcgcta gaaccaaata
    14641 ctccacctgc aataaactca acaatggcat aagtcaaatt taagaaaaaa gccacccaaa
    14701 cagcatattt tgccttcata tttctcattc ctttgttata atagatttat gaacaccttg
    14761 ttcattatca ttatccacta aaactcaaag aaaggataga agcaaaacgt cagctttatt
    14821 cagttctgaa caatttgcct caagtgtcca tatgactaac attgaccgcc gtatcagcaa
    14881 aaccaaaaaa gccatctatc aagcttttat acaacttttg aatgctaagg gctacgaggc
    14941 cactactgtt caggatatca ttgatctcgc agatgtggga cgatccacct tttactgtca
    15001 ctatgagagt aaggagcttc ttctggacca gctctgccgc tacctcttcc atcatctttt
    15061 tgaaagagag caagccattt caaccgaaga ttacctagcc cacctctttc tgcattttca
    15121 gaaaaaccaa gaccatatca ccagtctact attctccaaa aatgactatt tcctccgtca
    15181 actacagaga gagcttgagc accacgtcta tcccatgctg gctgctgatt tgaagcaaac
    15241 tcacccaagt ctacctactt cttacctcca acacttggtc gtgtccaact ttatcgagac
    15301 attgacctgg tggctcaaaa aaggtcaaga tttcacagac caggaagttg tccaatttta
    15361 tctagacctt ctcattccta aaaattgaat atagagtaaa gcttcagttg tcttatttct
    15421 aggttactga gttttttatc ttttcaacaa caaaagagga cccgccgatc ctctttttca
    15481 tactataaat ccttgattat caattatatc tgttttaatc gaaatctcaa aacagcactt
    15541 tcaaacatct tttcctagtt aagtaaatca gtattctgct tagctgcctt gctccattga
    15601 taccaaccaa ctagactgtt aatgagataa attagatatt tcccttgaat ttgcaggctt
    15661 tctccccacc agagatagat tgaaaagaca ttggtagccg cccagaatat ccactgttca
    15721 cggtaaacag ctgtcatgag gatttgccct accccattgg ttgcatctgt gattgaatca
    15781 cgatagggac gattggcacc aatagactga taaatgaagc caaaggccaa ccaccaaagc
    15841 acactaatgg aaagatactt tgtccagccc ttgccgtcca gtttacgcgc gacaaactcc
    15901 tgcttttcct tcttaaactg tgcctgataa atccaaacta gaagtccaat tggctgcatg
    15961 actgtgaagt aaagtgtcgt cagcacctca ccataaaagc ctttctgtag ggccaaaata
    16021 aggtaaataa cagagttaat caagccaaaa agataattac ttgctcgacc ttccgataca
    16081 aagattacac agataatccc tgtcaagcta caaatcatcc caatccagtc aacaatacga
    16141 tgttcgtaaa ccaactccag ccagagagga aaacttccta aaaccagcaa ataaatccac
    16201 tgggcaaaac tacgatgggc aaagaggtca tcccagatag ccttcatagt tcctgaaaat
    16261 cctaaatcag ccatagccgc aaccatacga cggtaaccac ctgacatttc acctagggtt
    16321 gttttgatat tttcaatttt cttttgcaaa taagtatgca tcatttctcc ttttgttttt
    16381 aaagagccgt gtctggatag actttcggac gcaacgctct attagataat gaactgccta
    16441 tacacaagat ttctaacctt agtcgacatg agctgaaacc tcttatttgt taagtagttc
    16501 acaaaatatt atacacctat tttatgaata gtcaactgtc tttacagtaa aattttagaa
    16561 aatcatgaaa attttctctt tctttccatt ttaagtgaca ttcagtcatt ctcacatcaa
    16621 aaaagcccag acgaaattgt ctgagcattc ttttatctag tcgtttaagg aagttgagtt
    16681 cagtatgttt aaagtctctg tcccatcatt tcttcaacaa accttgttct tggagaaact
    16741 ccttggctac ttgctttgct gacttgcctt caacaccgac ttggtagttg agctggctca
    16801 tctggctttc tgtaatctta ccagccaatg tattaagaac tctttccaac tctgggtgtt
    16861 tcttgagaag agcttctttc atgagtggag ccccttgata aggtgggaag agttgcttgt
    16921 catcttccaa gacctgtaaa tcataacgct ccaattccgc atcagtcgaa taggcatccg
    16981 tgatttgaat atcccctgac tgaattgcct gataacgaag agctggctgc atagtcgcca
    17041 cattgagatt gagaccatac attgattgca agcccttatt tccatcttca cggtcgttaa
    17101 actcaagcgt aaagcctgcc ttcagctgcc cttccacttt tttcaaatcc gaaatggtct
    17161 tcaagccata ttcttgagca atctttttcg gaacagctac agcataggta ttttgataag
    17221 acataggttt gagataggct agatgatcct gcttggcaat gccatcacgc gccacttgat
    17281 aaacctgctc tggctcatga ctcaccttgg gtgatggttg aagcaaactt tcagtcaccg
    17341 taccggtaaa ttcaggataa atgtcaatat cacctttttt cagagcttca taaaggaaac
    17401 ttgtcgtccc aaaattcggt ttaacagtcg cagtcatgct ggtattttct tcaatcagca
    17461 atttatacat attagccaaa atttctggtt ctgggcctat tttcccagca ataatcaagt
    17521 tttccttctc tttttgagcc aaaagggctg gactataaga cagacccagt aataaagcca
    17581 ccaaggcaaa accagagaaa atcgtccgca atttcgcttt ttccattact tttagtagga
    17641 agttaaaggc aatagctagc actgcagaag aaagggcccc aatcaaaatc aaactggcat
    17701 tattacggtc aattcccaac aggataaagg aacccagtcc ccctgcccca atcaaggtcg
    17761 ccaaggtagc cgtaccgata atcaagaccg ctgccgtccg aattccagac atgataacag
    17821 gcatggcaag tggaatttca aacttcttga gacgctccca tctggtcatc ccaaaggcaa
    17881 tcccagcctc ttgaagactc ggatcaattc ccttcagccc agtaatagta ttttgtaaaa
    17941 tcggaaaaat cgcataaatc actagagctg tcaaagccgg caaggtccca attcccatca
    18001 aagggataaa gagccccaac aaggccagag acgggatggt ctggaaaatc cctgcaatct
    18061 gtaagaccca atccgccagc ttctcatgat agcgaagaaa aacagccaag ggaatcgcaa
    18121 gcaaaatagc tagtaacaag gtcaaaagtg acaactgcaa atgttgagat agagctgtca
    18181 accaatcact aaaacgatcc tgaaaagttg caattaaatt agtcatgaac actacctcca
    18241 aacaagtctg ctacaaagtc tgttgcaggc acttttaaaa ttgtctcggg attcgctacc
    18301 tggcgaattt ctccatcctg caagacagca atacggtccg ccaacttcaa ggcttcatcc
    18361 gtatcatggg ttacaaaaat cgttgtcatc ccaaactctt tatgcaattc ttttgtcaga
    18421 acctgcaact gttttctcga aatagcatcc aaggccgaaa agggttcatc catgaggaaa
    18481 atcttgggct gaccaatcat agctcggaca ataccgaccc gttgctgttc tccaccagat
    18541 aattcactag gtaagcgatg cccatactcg gctactggta aaccaacctt agccaaaagc
    18601 tcttctgttt tcttcgtaat ttcttccttg ctccacccct tcatttcagg aatgagagca
    18661 atattttccg caactgttag atttggaaaa agagcaatag cctgtaaaac ataaccagta
    18721 gaaagacgaa gttcacgctc atcatagtct ttgatgcgct tcccatccat ataaatattt
    18781 ccatcagttg gttccaaaag acggttaatc atcttgagca tggtcgtctt acctgaccca
    18841 gaaggaccta ctaaaaccat aaattcccca tcctcaatct gtaagttgac atctctcaag
    18901 acatcctttt ctgtgtagcg cagtgctaca tttttgtatt caatcattct ttgtcctcaa
    18961 tttaaaactt ccctcgatta gtcaagtctt ctaccttagg catgacttcc ttattatccc
    19021 aatgctccac aattttcccg ttctctaaac ggaagatatc gtactgggca taagcaacgc
    19081 catcaatctg agtctgacca tagctaacca catagtttcc ttgtcctaag agttggaaaa
    19141 caaagtcaaa agtgacacta tattcagcca catagttttt ataagcagca cttccttgtc
    19201 caatctcatg attatgctga atcaaatcgt ctgccacata atcactccac tgctctagct
    19261 ccccattttg gaaaatttct gtcaagaaac ggcgaaccag ctttttattt tctgctttct
    19321 tatccaaatc cttgatttca aaatctccaa aaatttgatc tagttggtca ttttcaggtg
    19381 ttcgatagta gtcaatgaca tcccaatgct caacaataca accattctca tcctcacgga
    19441 aagtatccgt cgtcacccat tgagcttctc caccattcag atattgatga acatgaacaa
    19501 agaccagatt gccatcctca atggtgcgga caatcttaat ctgacgctct ggatgacgct
    19561 caaagaaatc tgcaaagaag gctgcaaatc cttctttccc gtcaggaaca cctgtcgaat
    19621 gttggatata ggtatcccct acagactggg cttgagcctc agcaactcgc ccgtcttgaa
    19681 tggcatggat gtataggttg tgagcatttt tcacttgttg tgacatattc taaacctcat
    19741 ttcccttctc tttcagattc gccaaaattc tttcttgaaa accttcaaat tggtgaattt
    19801 cttcctctga aaatcctttg taaaagatag tatccaattt ctgactgaca cgatgcccca
    19861 cttctttctg gaacttgcct aactccgtta aaactaaata cttcttacgc ttgtcttttc
    19921 cacacggact aacaattaca agcttttgtt cctctagctt ttttatcata gtcgtcagcg
    19981 tattattcgc aagcccagtc gcaagcgcga tatctgtcgc agttgcgcag ccagtttcac
    20041 tattccataa aaccgctaaa atcttgccct gttcacccct ataaagagcc tcaggatctt
    20101 gactcagtaa cttttgaaaa atccgcccat tcaacaaacg aatatgatgg gctagcaaat
    20161 gactatcttt cataacacct ccaatttatt tcgatatcga aatgaataaa ataattgtaa
    20221 cactcatcgt tctaactgtc aactatttcg atttggaaat aatttttgat aattatccac
    20281 accaccatac tccggctcaa ctaactttta acgagagttt ctaaactcct tcgtcctcca
    20341 gtctacaaaa gccttccatt cgtactatcc tatattttat gaggggacac atttttccta
    20401 tcagaccatt tattttaaag atagaagtaa atcataattg cttccatctg ttcttttata
    20461 gtatattgaa gttagactag agcactgtat cttctaaaac attgatagaa agcgatttga
    20521 atttcccaat caatttgttc gtatttatag cattttgaaa ctagaatagg acaccatgac
    20581 tgctaaaaga tttctataaa ttcatttgat tttcctaatc aatttgttca tatcttattt
    20641 cattccgcta taatttcacc ttaccctatc tttttcgtag cacccttcaa acagcctatc
    20701 ccctaccgtt tgacgattcc tcacttcgct ccacttccat tacagaagtt tcttcactac
    20761 tatgggctcg gctgacttct catgattcct tgttactact atttgaacgc tcacgagata
    20821 gatcttacaa aaaatgcttt gatccacaat ggaatcaaag cattttaaag agttcctcat
    20881 acataagcgc agaagtcgca gttcctctgt acttggcttc ttctcttttg ataaagcgag
    20941 ccaagttgag caactcaggt gctggatgtt tgggatttag gagcaattca cgattgacca
    21001 ggcctgagag acgaactgcc tgcaattgct catttgtagt aggcagtttt ttagcagtct
    21061 ctaggagagc agcaactaaa tcttcactca aatcatgtcg agcatgattg taaagatctt
    21121 ttataaggct ttctaggttt ggttctgcca tccctaccac ctcccttatg gtttaataat
    21181 gtttaatcaa atcaaccgtt gaacgatcca atttcttcac caaggcttgt aagaaagctt
    21241 gcgcttctag gaagtcatcc attgcataga gggtttggtg agaatggata taacgagcgc
    21301 agacaccgat agttgttgat gggacaccac catttttcag atgagctgca cctgcatctg
    21361 ttccgccttt accacagtag tattggtact tgataccagc ttcttcagcc gttgtcaaaa
    21421 gaaaatcctt catccctggg agaagcaagt gacctggatc atagaaacga atcaaggttc
    21481 catctccaat cttgccttga ccaccgtaga catcacctgc tggtgagcaa tcaactgcga
    21541 ggaagacttc tgggtcaaac ttggttgtag aggtatgagc gccacgcaga ccaacttctt
    21601 cttggacgtt agaacccaga tagagttcat tgccgagttt ttgacctgat aaagcttcag
    21661 ctagctcgct taccatgagg acaccgtagc ggttatccca agcttttgag atgatatttt
    21721 tttcattggc tgtcaaaatt gcagaactat ctggtacgat ggtatcacca ggacggatgc
    21781 caaaactttc tgcctcagcc ttgtccgcaa aaccaccatc aaaaacgata tcggcaatgg
    21841 ctggcatggt tggtcccccc tttccacgag tcaaatgcgg aggaacagaa cctgaaatca
    21901 caggaatttc atgaccatca cgagtcaaga gtttgaaacg ttggctgcta accaccatgg
    21961 ggttccagcc accgatttct acgacacgga aggtaccatc tggcttgatt tcgctgacca
    22021 taaaaccaac ttcgtccata tgagaagcga ccaagacgcg cggtgcatcc acagcttctg
    22081 aatgtttgat accaaaaata ccacccaagc catctgtcac cacttcatcc acatgcggtg
    22141 tcaacttttc acgaagataa gcacggacag gcgcttcatg acctgagact gcagcaagtt
    22201 ctgttacttc tttaattttt gaaaataatg ttgtcatttc agttccttct ttctttcatc
    22261 cattttacca ctttttatag gagaaggata gtgggaaggt ggatttctaa gttagtatct
    22321 tagtcctgct ctatcttaga aaaggatagt attctcttgc atgtagtgca aaatctagta
    22381 aacattccaa aattaactcg aatatttatt tccaaacaaa aaaacaatac accatcaaag
    22441 ttgtttgtat ttttcatgaa atttacagaa aatagttgac ttccctttct tctttcttta
    22501 aatatatagt tggttgagtt tggaatagta cgctgtagct gctgaaacat ttctagaaat
    22561 taatttgact ttcctaatag agttgttcat atcttatttc aatttactat agtacaaaac
    22621 tagaaaagga aaaaatcatg accaaaacaa ttcttgttac aggtgggact agctacattg
    22681 gctcccatac cgttaaagct cttttaaatg ctggctataa ggtgcatgtc ctcgataatc
    22741 tctccacagg aaatcgagcg gctgtggata gtcgtgctag ctttaaagaa ctggatgttt
    22801 atgatgctag cgccttaaag gcttacttag aagaaaatca gattgatgct gtcctccatt
    22861 gtgcaggtga aattgttgtg agcgaaagta ttgaaaatcc aagtaaatac ttcactgcca
    22921 acgttgctgg tatgaaccaa gttctcaaag tcttatctga agttggcatt caaaaaatca
    22981 tgttctcttc gactgcttct ctctatggaa ataactgtat tgacaagcca gtgactgaag
    23041 ataccctgct cgaccctgtc aacccttatg cagagacaaa actgatgggc gaacgaatga
    23101 tttactggat ggccaatcgc tacgactgga aatatgttat tttccgttac tttaatgttg
    23161 ctggggctga aatggatgct tcaaacggtc tgcgtgtgaa aaatccaact cacatcattc
    23221 caaatatcaa caagaccgca ttgggacaaa atgatagctt aaaaatattt ggagatgact
    23281 acgatacacg tgacggttca tgtattcgag attacattta tgtcttggac cttgcatagg
    23341 ctcatgttaa aggaatgaac tacctatttc aagaagacag ttcttctcaa atctttaact
    23401 taggaactaa aaaaggctat actatcaaag aaatctttaa aactgctgaa gaattactaa
    23461 attaaaaaat cccacacgag attgttgctc gacgtgctag tgacccagcc agtgtcctag
    23521 cagatgcttc aaaagctaaa caatatcttg attggacggc tagctactcc ctcgaagata
    23581 ttattttatc agattaaggt aaaaacattt tagaatagga taacaaagga gaaagtcttg
    23641 ttgctctctc cttgttgtat ttattaaatt gtcagaaatt ttattgacag attagaaaaa
    23701 taatgttaca tttatatccg cagatatctt tcgataccaa atctacatga aaggacgggg
    23761 tatgaaactt tctcattatt taattggctt acttctactc ctagtctttc tctctattag
    23821 cattgggacc agtgattttt catggggaaa gctatttgat ttcgaccagc agacatggct
    23881 cctctttcaa gagtcccgtc tcccaagaac tatcagtatt ctcctgactg cctctagtat
    23941 gagtatggca ggccttctca tgcagactat tacccaaaat cagtttgctg caccgagtac
    24001 agttggaacg actgaagccg ccaaactggg aatggtgctg agcctttttg tctttccatc
    24061 ggctagtctg acccaaaaga tgctcttcgc ttttgtttca tccatcgtat tcaccctctt
    24121 cttcctagcc tttatgacca tttttactgt aaaggaaagg tggatgttgc ctctgattgg
    24181 gatcatctat agcgggatta tcggctcagt cacagaagtt atcgcctatc gtttcaatct
    24241 ggttcagagt atgactgcct ggacccaggg ctccttctcc atgattcaga cccatcagta
    24301 tgagtggctc ttcttaggcc tcatcatcct gataaccgtt tggaaattat cccaaacctt
    24361 caccatcatg aatctgggga aagaaatcag cgagagtttg gggatttcct actccctact
    24421 tgaaaaactg gccctctttc tggtggcgct aacgacaagc gtcaccatga ttaccgtggg
    24481 tggcctacca tttctcggag ttatcgttcc caatcttgtt cgcaagcgct atggagataa
    24541 tctaagtcaa accaaactca tggtcgcact ggttggtgcc aatctagttc tggcttgcga
    24601 tatcctatcc cgagttctga ttaggcccta tgagttgtct gtcagtctct tgctaggaat
    24661 catcggtagt ctcggcttta tcctacttct ctggagaggg ggacgaaaag atgcagacta
    24721 aaagcaaaca taccaagctc ttctggattc tcattattct tgccatcgga gcttgtcttc
    24781 tctacttttg gcccatcact cacttgtcag cctttgcttg gaagttgcgt tcccaaaaga
    24841 tcatcgttta tctcttggta gccatcgcga ctgggatttc gaccattagt tttcaaaccc
    24901 tgacggaaaa tcgcttcctg acgcctagta ttttgggaat tgaatccttc tacgtcctac
    24961 tacaaaccct actactggtt tttgaaagca agtttcttca acttggcaaa tcccctatct
    25021 tagaattcct agtcttactt cttgtccagt ccctcttctt tctcacctta caaggttact
    25081 tgaagacact gatgaagcaa gacctggtct tcatcctgct gatctgtcta gcgctcagaa
    25141 gtctctttcg aaatatcagc accttccttc aagtcctaat ggatccaaac gaatacgata
    25201 aactgcaaaa tagtcttttt gcctcctttc aacatctcaa cacttccatc ctagccatcg
    25261 gttctctgat catcctcgct ttgacaatct ttttctttcg aaaagcagtc gttctagatg
    25321 tcttgcacct gcaaagagaa acggctcaga tattgggact cgatgttgaa aaagaacaga
    25381 aagagctcct ctggggaatc gtgcttttga cctcaacggc cactgccttg gtaggaccta
    25441 tggccttctt cggctttatg ctggccaacc tcacctacct gattgtcaaa gactatcagc
    25501 acaagttact ctttatagtg gccattctgg ttggatttat tagcttaacc ttggggcaag
    25561 ccttgattga acgagtcttt gcactggaaa ttcgtatcag tatgatcatt gagagtgtgg
    25621 gtggcttctt attctttatc ttactatata ggaggtctcg tcagtgaaac tggaaaacat
    25681 tgacaaatcc attcaaaaac aggatatttt gcaaggcatt tcgcttgaag tcagtcctca
    25741 aaaactgaca gcctttattg gtccaaatgg tgctggaaaa tcgactctcc tctccatcat
    25801 gagcagacta accaagaaag atcagggagt tctcagtatc aaaggacgtg aaatcgagag
    25861 ctggaattcg caagaactgg cccaagaact aaccatccta aaacagaaaa tcaattacca
    25921 agccaaattg actgttgaag aactggtcag ttttggacgt tttccctaca gccgaggtcg
    25981 acttagatca gaagactggg aaaaaatccg agaaactctg aactatttgg aactgaccaa
    26041 cttaaaagac cgctacatca atagcctgtc aggggggcaa ctccagcgcg tctttatcgc
    26101 tatggtactg gcccaggata cggactttat cttgctggac gaaccactca acaatctcga
    26161 tatcaagcaa agcgtcagca tgatgcagat tcttcgacga ctggtggagg aactcggcaa
    26221 gaccattatc atcgtcctcc acgatatcaa catggccagt cagtatgcag atgaaattgt
    26281 cgccttcaag gacggccagg tctttagcaa gggaagcacc gatcaaatca tgcaggctga
    26341 cctgctcagt caactttatg agattcccat cacgctagct gatatcaatg acaaaaagat
    26401 ctgtatctat agctagtaac ataaaagctc aagttagaga accttcagtc tcttagtcaa
    26461 taagattaag agactcccta aatcgttatc acattttaaa aaggagaaat tatgaaaaca
    26521 tcccttaaac tttatttcac tgccctagtg gccagcttct tgctcctact tggtgcatgt
    26581 agtacaaact caagcactag tcagacagag accagtagct ctgctccaac agaggtaacc
    26641 attaaaagtt cactggacga ggtcaaactt tccaaagttc ctgaaaagat tgtgaccttt
    26701 gacctcggcg ctgcggatac tattcgcgct ttaggatttg aaaaaaatat cgtcggaatg
    26761 cctacaaaaa ctgttccgac ttatctaaaa gacctagtgg gaactgtcaa aaatgttggt
    26821 tctatgaaag aacctgattt agaagctatc gccgcccttg agcctgattt gattatcgct
    26881 tcaccacgta cacaaaaatt cgtagacaaa ttcaaagaaa tcgccccaac cgttctcttc
    26941 caagcaagca aggacgacta ctggacttct accaaggcta atatcgaatc cttagcaagt
    27001 gccttcggcg aaactggtac acagaaagcc aaggaagaat tggccaagct agacaagagc
    27061 atccaagaag tcgctactaa aaatgaaagc tctgacaaaa aagcccttgc gatcctcctt
    27121 aatgaaggaa aaatggcagc ctttggtgcc aaatctcgtt tctctttctt gtaccaaacc
    27181 ttgaaattca aaccaactga tacaaaattt gaagactcac gccacggaca agaagtcagc
    27241 tttgaaagtg tcaaagaaat caaccctgcc atcctctttg tcatcaaccg tacccttgcc
    27301 atcggtgggg acaactctag caacgacggt gtcctagaaa atgcccttat cgctgaaaca
    27361 cctgctgcta aaaatggtaa gattatccaa ctaacaccag acctctggta tctaagcgga
    27421 ggcggacttg aatcaacaaa actcatgatt gaagacatac aaaaagcttt gaaataagcc
    27481 acttcaaact caa
//
LOCUS       DAVBCG010000016        25312 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBCG010000016 DAVBCG010000000
VERSION     DAVBCG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.016"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            218..448
                     /locus_tag="VQ495_000560"
     CDS             218..448
                     /locus_tag="VQ495_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2530554.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            852..1565
                     /locus_tag="VQ495_000561"
     CDS             852..1565
                     /locus_tag="VQ495_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2530555.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            1623..2225
                     /locus_tag="VQ495_000562"
     CDS             1623..2225
                     /locus_tag="VQ495_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530556.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2361..3014
                     /locus_tag="VQ495_000563"
     CDS             2361..3014
                     /locus_tag="VQ495_000563"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2530557.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3024..3488
                     /locus_tag="VQ495_000564"
     CDS             3024..3488
                     /locus_tag="VQ495_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2530558.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            3501..4769
                     /locus_tag="VQ495_000565"
     CDS             3501..4769
                     /locus_tag="VQ495_000565"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2530559.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            4783..5043
                     /locus_tag="VQ495_000566"
     CDS             4783..5043
                     /locus_tag="VQ495_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530560.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5054..6415
                     /gene="pdrM"
                     /locus_tag="VQ495_000567"
     CDS             5054..6415
                     /gene="pdrM"
                     /locus_tag="VQ495_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2530561.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            6569..7537
                     /locus_tag="VQ495_000568"
     CDS             6569..7537
                     /locus_tag="VQ495_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2530562.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            7553..8545
                     /locus_tag="VQ495_000569"
     CDS             7553..8545
                     /locus_tag="VQ495_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2530563.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            8763..9806
                     /locus_tag="VQ495_000570"
     CDS             8763..9806
                     /locus_tag="VQ495_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2530564.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            9870..11555
                     /gene="lpdA"
                     /locus_tag="VQ495_000571"
     CDS             9870..11555
                     /gene="lpdA"
                     /locus_tag="VQ495_000571"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2530565.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            11619..12608
                     /locus_tag="VQ495_000572"
     CDS             11619..12608
                     /locus_tag="VQ495_000572"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2530566.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(12675..13745)
                     /gene="xerS"
                     /locus_tag="VQ495_000573"
     CDS             complement(12675..13745)
                     /gene="xerS"
                     /locus_tag="VQ495_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2530567.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14329..15879)
                     /locus_tag="VQ495_000574"
     CDS             complement(14329..15879)
                     /locus_tag="VQ495_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2530568.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(15895..16674)
                     /locus_tag="VQ495_000575"
     CDS             complement(15895..16674)
                     /locus_tag="VQ495_000575"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2530569.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(16661..17512)
                     /gene="ylqF"
                     /locus_tag="VQ495_000576"
     CDS             complement(16661..17512)
                     /gene="ylqF"
                     /locus_tag="VQ495_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2530570.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(17719..17895)
                     /locus_tag="VQ495_000577"
     CDS             complement(17719..17895)
                     /locus_tag="VQ495_000577"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2530571.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(17947..23838)
                     /gene="iga"
                     /locus_tag="VQ495_000578"
     CDS             complement(17947..23838)
                     /gene="iga"
                     /locus_tag="VQ495_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2530572.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24047..25225)
                     /locus_tag="VQ495_000579"
     CDS             complement(24047..25225)
                     /locus_tag="VQ495_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530573.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         7267 a         4857 c         5176 g         8012 t
ORIGIN      
        1 cttaacagat agtgtaaata aagataaact atttactggc taattaatca gttaaacact
       61 agttaaggag taatgatgaa aaaaagaaca atactattat tgatggccag tctgttagct
      121 cttgtcttag gagcatgtgg tttcttggac atattgatcc tggatcattc tcatcaggat
      181 tactctttac tgctatttta gaaactgggg tggtttgatg gaaagtattg gtattggtct
      241 tgttatcgtt tcacattcca aacacattgc agaaggtgtt gttgaactga ttagtaaagt
      301 agctaaagat gttccgatta cttatgtagg aggaaccgag ggcggaggaa ttggaacgag
      361 ttttgatcaa gtagataggg ttgtttccga aaatccagca gatactttac ttgccttttt
      421 tgacctaggt tctgctataa aatgttaagt aaatgatttc aaaatcagaa agggattgct
      481 ttgggcagtt cctttttaat gtaaatgtaa aaggagtaga atcatagtgt ttctaaattg
      541 tgaatcaatc aaaactgatt gtgatggggc tattctagct ttagaatcct tcaaaaatta
      601 aaatttaagg caatcaatta cttggaagag tatgaaagca tttagtttat aggaattcta
      661 ggcctagaat tacatatgta tttatgaaga taggggtgtt cgatagttag tattgttctg
      721 ttctgaaaga tttgagctgt cagttgtata gaaagtgttc gaattttttt taagtgatta
      781 aattagttaa ttgtatgagg ttctttatga tataatgttc ttaatgaatt ttcagaaagg
      841 aaaacctcaa attgttctac aaatttctac tcttcgacct cgaccacact cttcttgatt
      901 ttgatgctgc tgaggatgtg gctttgaccc aacttctaaa agaagaagga gttgcggata
      961 ttcaggctta taaagattat tacgttccta tgaacaaggc tctctggaaa gacttggagc
     1021 tgaagaaaat cagtaaacaa gagctggtta acacgcgctt ttctcgttta tttgctcatt
     1081 ttggacagga aaaagacggt agttttcttg cccagcgtta ccaattttac ctcgcccagc
     1141 agggacaaac actatcgggc gctcatgatc tcttggacag cctcattgag cgtgattata
     1201 acttgtatgc tgcgacaaat ggcattactg ccattcagac aggacgtttg gctcaatctg
     1261 gtctagcacc ttatttcaat caagtcttta tctcagaaca gttgcaaact caaaagccgg
     1321 atgctctctt ttatgaaaag attggccagc aaattgctgg atttagtaaa gaaaagacgc
     1381 tgatgattgg agattctcta accgccgaca ttcaaggtgg caataatgcg gggattgata
     1441 ctatctggta taatcctcat cacctcgaaa atcacacaca agcccagccg acttacgaag
     1501 tctattctta ccaagacttg ctggattgtt tagataaaaa tattcttgaa aagatcacat
     1561 tttaaaggag acgagctaat gactacaaaa aagctaatat tactattgaa gagtacattg
     1621 aaatgtctga agttgatttt aatgaagctg ttaattatga atttacatct gacacttgtc
     1681 aattagcaaa tagtatttat caatctcttt ttaagttttt tgataagaaa aatttctctg
     1741 gcgatttaat ttttacttgg aaatctccat cattagtcaa agaaggggat tatattggga
     1801 gaagggattc acaagtagat aatcttagag taataggaaa tatatttccg aattatctta
     1861 ctaatcgaaa atatagcctc aatatgaatc gtaatggctg tatgggagat tttcctcatg
     1921 acttttttga tatataccta gatcatgtag caaaatatgc ctacgaacaa aaagttaata
     1981 atattaaaga gtattatcct ttaaaaagag cgattttaca ccaagagaat gcattgtatt
     2041 ttcgattttt ttctaatttt gacgactttt tagaaaaaaa ttatttaaag actatatggc
     2101 aagtttctaa agaaactcct ttttctgaaa tggattttaa tatgtttaaa aatatttcag
     2161 aaaagataat cttcgaaaga ggtagtaaaa tgcttaatga tttaaaaagt aattataaaa
     2221 aatagccagt ctttataaac tgtattcacg tattcaaatc gtatatagtc gatttattaa
     2281 atctttagat aaactgtaaa aagtggtttc catagccact ttttgctata atagaggcag
     2341 taaaaatgaa ggagtcggct atggaacact cgtcttggca tgctttgatt aaggcgcaat
     2401 tacctgaggg ttatttcggg aaaatcaatc agtttatgga gcaggtctat tctcagggga
     2461 ttatttatcc acccaaggaa aaggtttttc aggctctctt gacaacactg cttgaagaag
     2521 ttaaggtggt aattctaggg caagacccct atcacggacc aggtcaagcg cagggcttga
     2581 gtttttctgt acctgactct atcccagctc caccatcctt gcaaaatatc ttgaaagaat
     2641 tgtcagatga tatcggagtt aagaaatctc atgatttgac agcttgggct gagcaaggag
     2701 tcttgcttct taatgcttgt ttgactgttc ctgctggaca ggccaatggt catgctggtc
     2761 aaatctggga gccttttact gatgctgtga ttcaggtggt caatcatcta gatagaccag
     2821 tcgtttttgt actctgggga gcttatgcac gtaagaagaa ggccttagtt accaatcctc
     2881 atcacttgat tatcgaatca gcccatccaa gtcctttgtc ggtttataga ggattttggg
     2941 gttccaagcc cttttccaag gccaatgcat tcttaaaaga gacaggacaa gagccaatcg
     3001 attggcttag ataaggagaa aatatgcctc agttagcgac gatttgctac attgataatg
     3061 ggaaagaact gctcatgctc catcgtaata agaaacccaa tgatgtccat gaagggaaat
     3121 ggattggtgt gggtggtaag ctagagagag gagagacgcc ccaggaatgc gcggcgcgtg
     3181 aaatccttga agaaacaggg ctcaaagcca agccagttct aaaaggtgtc atcacttttc
     3241 ctgaatttac accagattta gactggtaca cctatgtttt taaggtgacg gagtttgagg
     3301 gcgacttgat tgactgcaat gaggggacgc tagaatgggt tccctatgac gaggttttga
     3361 gcaagccgac ttgggaaggt gaccacacct ttgttgagtg gcttttagag gataaaccct
     3421 tcttttcagc caagtttgtt tatgatgggg ataaattgtt ggatacccaa gttgatttct
     3481 atgaataaag gagaaagcag atgctactaa tcaaaaatgg tcgtgtaatg gatcctaagt
     3541 ctggtttgga tcaagtttgt gatgtcttag ttcaagatgg gaaaattatc aaaattgcgc
     3601 ctgagatcac ggaagaagga gcagaaacga ttgatgctac tggtcttgtg gttgctcctg
     3661 gtttggtcga tattcatgtg catttccgtg aacctggtca aacacataaa gaagacattc
     3721 atactggtgc cctggcagcc gctgcaggtg gttttactac tgtcgtcatg atggctaata
     3781 ccagtccaac catttcagac gtggagactt tgcaagcagt tctccagtca gctgccaaag
     3841 agaagattaa tgtcaagaca gttgcgacca ttactaaaaa ctttaatggt aaaaacttga
     3901 ctgactttaa ggcgctctta gaagctggtg cggttggttt ctctgatgac ggtattccgc
     3961 ttgagagcag taagattgtc aaggaagcca tggaggaagc caaaaaactc aataccttta
     4021 tcagccttca tgaggaagat ccaggtttga acggtgttct tggctttaat gaaaatattg
     4081 ctagagaaca tttccatatc tgcggtgcta ctggggtggc tgagtacgct atgatggcgc
     4141 gtgatgtcat gattgcctat gcaactaaag cccatgttca catccagcat ttgtctaagg
     4201 aagaaagtgt taaagtagtg gagtttgctc aggggttagg tgcagaagtc acagcagaag
     4261 tagcgccaca gcatttctct aagacagaag cacttctttt aacacaaggt agcaatgcta
     4321 agatgaatcc accgcttcgt ttggaatcag accgtcgtgc cgttatcgaa ggtctcaaat
     4381 caggtgtcat cacagttatt gcgactgacc acgcgcctca tcatgtagat gaaaaaaatg
     4441 ttgaggatat taccaaagcg ccatctggta tgactggctt agaaacatcc ctgtctctcg
     4501 gcttgaccta tttagtagaa gctggtgagt tgagcttgat ggaattactt gaaaaaatga
     4561 catacaaccc agccaagctt tacaactttg aagcaggtta cttggctgag aatggtccag
     4621 cagatatcac tatttttgat gccaaggctg accgccttgt ggactcccat tttgcttcca
     4681 aagcagctaa ttcaccattc atcggtgaaa ccttaaaagg gcaggttaaa tataccatct
     4741 gtaagggaca aatcgtctat caagcttgat aggagttaga atatgaatta ttttcgaaaa
     4801 cgtagggaga gacaagcgaa atctaatagt ggaatttact gtcccgcagc taatcgttcc
     4861 aaaattcaat atgaaactaa agaaaaagct gaccatgcgg ttcaatatga taacggaggt
     4921 ttagttagaa gctattattg tcgtacatgt gcgtgttggc atactacttc aaaatctaat
     4981 aaacctccgc taagtttgaa aaactatggt ctgaaactct ttggtttaga taaatcagaa
     5041 tagaaagaga agtatgtata agacaaagtg tttacgagag aagttagtat tatttttaaa
     5101 aattttcttc ccaatcctga tctaccaatt tgccaattat tctgcctctt ttgttgatac
     5161 tgcaatgaca ggtcaataca acactatgga cttggctggt gtatctatgg caaccagtat
     5221 ctggaatcct ttctttacat ttctaacagg gattgtgtca gccttggtgc ctatcattgg
     5281 tcaccatctt ggtcgaggca aaaaggaaga agttgcgtct gatttttacc aatttattta
     5341 tttggccttg ggcctatctg tggtcttgct ggggatggta cttttcttgg caccaacaat
     5401 cttgaatcat attgggttag aagcagcagt agcggcagta gcggttcgct atctttggtt
     5461 tttatctatc gggattatcc ccttgttgct ctttagcgtc attcgttcct tgctggattc
     5521 gctgggcttg accaaactgt ccatgtacct catgcttttg ttactccctc tcaatagcgg
     5581 atttaactat ctcttgattt acggtgcctt tggtgttcca gaactgggag gggctggtgc
     5641 tggtttagga acatccttgg cctactgggt cttgcttggg atttctgttc tggttttatt
     5701 taaacaggag aagctcaaag ccttacacct tgagaaacga attccactta atatggataa
     5761 aattaaggaa ggagttcgtt taggtctgcc tattggggga actgtcttcg cggaagtggc
     5821 tatcttttca gtggttggct tgattatggc taagttttcg tccttgatta tagctagtca
     5881 ccagtcagct atgaactttt caagtcttat gtacgccttt cctatgagta tctcatcggc
     5941 tatggctatt gtcgtttcct atgaagtggg agccaagcga tttgatgatg cgaaaaccta
     6001 tattggtcta ggaagatgga ctgccctcat ttttgcggcc ttcaccttaa ccttccttta
     6061 catttttagg ggaaatgtgg ccagtcttta tggtaacgac ccaaaattta tcgatttgac
     6121 agcgcgtttt ttaacttata gtcttttctt ccagttagca gatacctttg cggcgccgct
     6181 tcagggaatt ttgcgggggt ataaggatac agttattcct ttttaccttg gtttgcttgg
     6241 ttattggggc gtagcaatcc ctgtggctac gctatttgat tccctaacag attttggagc
     6301 ttattcttac tggatcggct tgattattag tttgattgtg agtggggcgc tctaccgttg
     6361 gcgtttaact gtgattatga agagatttga atctttagca aaatccaaac gctaaaaagt
     6421 ttgtgaaaaa atattcttga taagaaaatc ccttgttttc acggggtttt cttttttatt
     6481 ttgtgaaatt tcttttagaa aaactttgac agtatttgcc aaaaacggta aaatagtaag
     6541 gtaagaagaa agttagaaag gcaagaagat gtcaacttta gataaaaatc ttttgctaga
     6601 aatgttccgt aagatggaag aaatccgtcg catggactta aaaattgcac aattagtaaa
     6661 gaaagggaaa gtgccaggaa tgacgcactt ttctgttggt gaagaggcag ctaacgtggg
     6721 ggctatgtta gctctcaatc cagatgatct gattacctca aaccaccgtg gacacgggca
     6781 agctattgct aaagggatcg acctcaacgg aatgatggct gaaatccttg gtaaatacac
     6841 tggaacctgt aaagggaaag gtggatctat gcatatcgct gaccttgatg ctgggaacct
     6901 tggtgccaat ggtatcgtag gtggtggtat ggggatcgct gtcggtgcag ccctcagtca
     6961 gcaaatgcaa aataccggta aaatcgttgt ctgcttcttt ggagatggtg cgaccaatga
     7021 aggtgttttc cacgaagcag tgaacatggc ttctatctgg aacctgccag tcattttcta
     7081 ttgcattaac aacggttacg gtatctctgc ggatatcaag aaaatgacca atatagaaca
     7141 tatccatcaa cgtagcgccg cttatggaat tcctggaatg ttcatcgaag acggtaacaa
     7201 tgtcatcgat gtctatgaag gatttcagaa agctgtagac catgttcgca gtggcaatgg
     7261 tccagtcttg attgaaagtg taacttatcg ctggctcggt cactcatcat ctgaccctgg
     7321 taaatatcgt acgcgtgaag aagtggaatt gtggaaacaa aaagatccaa tcgaaaacct
     7381 ccgcaattac cttattgaaa ataacattgc aagtgccgaa gaattggaag aaatccaagc
     7441 gcaagtaaag gaagcagtag aagcttctgt taaatttgca gaggaaagcc cattcccacc
     7501 gcttgaatca gcctttgaag atatttacgc agactaagga gaaagagata aaatggaaac
     7561 aaaaacaatg tccttccgtg acaccattat ccttgctatg tctgaggaaa tgcgtcgcga
     7621 tgaaaatgtg ttcttgatgg gagaagacgt cggtgtcttc ggaggagact tcggtacttc
     7681 tgttggaatg cttgaagaat ttggtccaga acgcgtccgt gactgtccga tttctgaagc
     7741 tgccatctct ggagcagcag caggagcagc catgacagga cttcgtccaa tcgtcgatat
     7801 gaccttcatg gacttctctg ttattgccat ggacaatatc gtcaaccaag ctgctaaaac
     7861 acgttacatg tttggtggta aaggtcaggt tccaatgact gttcgttgtg cagctggtaa
     7921 cggagttggc tctgccgccc agcactcaca atctctagag tcttggttta ctcacattcc
     7981 aggacttaag gttgtggcac ctggaacacc tgcggacatg aaaggcctgc ttaagtcttc
     8041 tatccgtgat aaaaacccag ttattatact tgagtacaag tcagaattta accaaaaagg
     8101 ggaagtgcca gttgatccag actacacaat tccacttggg gttggtgaaa tcaaacgcca
     8161 aggaacggat gtaacagttg ttacttatgg aaaaatgctt cgccgtgtgg ttcaagctgc
     8221 tgaagaatta gcagaagaag gaatttcagt tgaaattgtt gacccacgta cccttgttcc
     8281 gcttgataag gatatcatca taaactcagt gaagaagact ggtaaggttg ttctggtcaa
     8341 cgatgcccac aaaacaagtg gctatatcag tgaaatttca gctattattt cagaatcaga
     8401 agcatttgac tatctagatg caccaatccg ccgttgtgca ggagaagatg tgccgatgcc
     8461 ttatgcacaa aacctagaaa atgcaatgat tccaacagtt gaaagtatca aagatgcaat
     8521 ccgaaaaact tataacaaag aatagagtta cataagataa gttatgtaaa taaaataaag
     8581 ctgacgagaa actttgtatc ttttaggtgc agagctcttc gtcattaata tcttatattc
     8641 gagcacgggc taaaagctcg gaaaaaagat aaatctcctt ggcttcatcg aagccatcgt
     8701 cgatttccta tttttcagtc gcttttgacg ccctttgtat cataaataga attggagagg
     8761 tcatggctga tgacaagcta agagcgactc ctgcagctag aaagttagcg gatgatttag
     8821 ggataaacct ctacgacgtt tctggctcag gtgcaaacgg tcgtgtccac aaagaagacg
     8881 tggaaactta taaagacaca aacgtggttc gcatttcgcc acttgcaaaa cgaattgccc
     8941 tcgaacataa cattgcttgg caggaaatcc aaggaaccgg tcatcgtggt aaaatcatga
     9001 agaaggatgt tttggccctg cttcctgaaa atatcgaaaa cgatagcatc aaatctcctg
     9061 ctcagattga aaaagtggaa gaagtccctg ataacgtaac accatatggt gaaatcgagc
     9121 gtattccaat gacaccaatg cgtaaggtta ttgcccaacg catggttgaa tcttacttga
     9181 ctgcgccaac cttcaccctc aactatgaag ttgatatgac tgaaatgttg gctcttcgta
     9241 agaaggttct tgagccaatc atggaagcaa ctgggaagaa gactactgta acagaccttc
     9301 tttcgcttgc agttgttaag actcttatga aacacccata catcaacgct tcattgacag
     9361 aagatggcaa gactattatc actcacaact atgtcaatct tgccatggca gttgggatgg
     9421 ataatggatt gatgacacct gttgtttaca atgctgagaa gatgagtctt tcagaactag
     9481 ttgtagcctt taaggatgtt attggccgta ccttggatgg taaattggct ccaagtgagt
     9541 tacaaaattc aacattcaca atcagtaatt tgggaatgtt tggtgttcag tcctttggcc
     9601 cgattattaa ccaacccaac tcagctatcc ttggtgtcag ttcgacaatc gagaagccag
     9661 ttgtcgtcaa tggtgaaatt gtgattcgcc caatcatgag tttaggatta accattgacc
     9721 accgtgtcgt agatggtatg gctggtgcta agtttatgaa ggacttgaaa gagttgattg
     9781 aaaccccaat ctcaatgttg atttaagagc aagagtattc atttttaaga tatagataaa
     9841 ggaaggaaga catggcctta gaaataatta tgccaaaagc cggcgtggat atgacagaag
     9901 gacaaatcgt ccaatggaat aaaaaagtcg gagaatttgt aaaagaagga gaaatccttt
     9961 tggaaatcat gactgataaa gtcagcatgg aattggaagc cgaagaagat gggtacttga
    10021 ttgccattct caaaggagat ggtgaaactg tccctgtaac ggaaattatc ggttaccttg
    10081 gtgaagaaag ggaaaacatc ccaacagctg gagcagcatc gccagaagct agccctgtac
    10141 ctgtagcaag tacctcaaac gatgatggta agagcgatga tgcttttgat atcgttgtga
    10201 ttggtggagg tcctgctggt tatgttgcag ccattaaagc tgcccaactc ggcggtaagg
    10261 ttgcccttgt tgagaaatct gaacttggtg gaacctgctt aaaccgtgga tgtattccaa
    10321 ccaagaccta ccttcataac gctgaaatta ttgaaaatat cggtcatgct gcaaatcgtg
    10381 gtatcgtgat tgaaaatcct aacttcactg ttgatatgga aaaactttta gaaactaaat
    10441 ctaaagttgt taatactctt gttggtggtg ttgcaggact tcttcgtagc tacggagtta
    10501 ctgttcataa aggaattggt acaatcacta aagacaagaa cgtcttggta aatggttctg
    10561 aattgcttga aaccaagaaa atcattcttg ctggtggttc aaaagtcagc aagatcaacg
    10621 tccctggtat gcaatctcca cttgtcatga ctagtgatga cattcttgaa atgaacgaag
    10681 tgccagaaag ccttgttatc atcggtggtg gagttgtcgg tatcgaactc ggtcaggcct
    10741 tcatgacatt tggttcaaaa gtgactgtta tcgaaatgat ggaccgtata gttccagcta
    10801 tggatgcgga agtttctaag aaccttcgct tgatccttga gcgtaaagga atgaccatct
    10861 tgactggtac taaactgcaa gaaatcattg aggaaaatgg tcaacttcgt atcaaggttg
    10921 aaggaaaaga cgatatcatc gcaagcaaag ctcttctttc aattggtcgt atgccagacc
    10981 ttgaaggtat tggagaggtt gagtttgaat tggatcgtgg ttgtatcaag gtcaacgaat
    11041 acatggaaac ttcagttcca ggcatttatg caccaggtga catcaacggt actaagatgt
    11101 tggctcacgc agctttccgc atgggtgaag tttccgctga aaatgccctt aaaggaaatc
    11161 atgcagttgc caaattgaat ttgactcctg cagccatcta cactctccct gaagtagcag
    11221 cagtaggttt gacagaagaa caagcccgtg agaaatacga tgttgccatc ggtaagttta
    11281 actttgctgc taacggtcgt gctattgcat ctgacgcagc tcaaggtttc gtaaaagtta
    11341 tcgctgataa gaaatacgga gaaatcattg gtgtacacat tattggtcct gcagccgcag
    11401 aattaataaa cgaagcatca agcatcatcg aaatggaaat cactgttgag gaaatgctga
    11461 agaccatcca cggacaccca acttactctg aagtgatgta tgaagcgttt gcagatgttc
    11521 taggaatggc catccattca cctaagaaaa aataaaacga agatagatta gactggaagg
    11581 ttatttccag tctctatcat ataaagtaaa gggatatcat gaaatacatt atcaatcatt
    11641 caaatgacac tgcttttaat attgccttgg aagaatacgc ctttaaacac cttttggatg
    11701 aggatcaaat cttcctactt tggattaaca agccatctat cattgttggt cgtcaccaga
    11761 acactatcga agaaatcaac cgtgattatg ttcgcgaaaa tggtattgag gtagttcgcc
    11821 gtattagtgg tggtggagct gtttaccacg atttaaataa cctcaactac acgatcatct
    11881 ccaaagaaga tgaaaataag gcatttgact tcaagagctt ctcaactccg gttatcaata
    11941 ccctggctca actcggggtt aaagctgagt tcacaggtcg taatgacctt gagattgatg
    12001 gtaagaagtt ctgtggcaat gcccaagcct atatcaacgg ccgtatcatg caccacggtt
    12061 gcttgctctt tgacgttgat ttgtcagtcc tcgcaaatgc ccttaaggtt tcaaaagata
    12121 aatttgaatc aaaaggtgtg aaatccgtcc gtgcccgtgt aaccaatatt atcaatgaat
    12181 taccaaaaaa aatcacagtc gaaaaattcc gtgatttact cttggaatac atgaaaaaag
    12241 agtacccaga gatgactgaa tacgtctttt cagaagaaga attggccgaa atcaatcaca
    12301 tcaaggatac taagtttgga acttgggact ggaactatgg taaatcacct gaatttaacg
    12361 tccgtcgtgg aaccaaattc actagtggta aggttgaagt ctttgctaac gttactgaat
    12421 caaaaatcca agacatcaag atttatggtg acttctttgg tattgaagac gttgctgcag
    12481 tagaagatgt ccttcgtggg gtgaaatacg aacgcgaaga tgttcttaag gcgctaaaaa
    12541 ccattgatat cacacgctac ttcgctggta ttagccgtga agaaatcgct gaagcggtag
    12601 ttggataaat caaaaaaaga agttggccaa cttctttttt tgatatttat ttacataatt
    12661 taaaatacgt gaaatcataa actatctaga gcattctttt gttcatcatt aacaatatgg
    12721 gtatagaggt cagtgacttg tgtgctggca tgtcctagtt ggtgactgac taaaacttgt
    12781 gatttagtcg catcatagag cctagttgct agcgtatggc gcagtttatg gggtgttaca
    12841 cgtactttaa aatcctctga gtatttagca accattttct caacgctaga agcatcgata
    12901 cgattaggaa cacctctgta gagagttaaa aaaagggctg tatctgtttt ttccgtttta
    12961 tagcgttgat tccgaatggc cagataattc tctaaataag gtttagcaaa agcagcgaca
    13021 ttgactgagt cacgtttgcc accttttcga gtaacatcaa taaccatcat ttttagattg
    13081 agatctctta gatctagatt aacagcttca gataagcgaa caccagatgc caagagaagg
    13141 gcaataatgg ctaaatctcg ttctttattt ttgttgaatg atgagagagc tcgatttgaa
    13201 agctgttgtg ggtgctcttg atcgatataa gttagaaaac cttctgtttc atcacctaga
    13261 aagagttttt gcttgatatt ttcagctctg gcagcaaggg tttctttctt tttcttggtt
    13321 gaaacttttt tcattacatt acgatagaaa taaggttccc cctgatcgtt ttcaacctcc
    13381 tcggttagat acttgtaaag actagaaagt gctgataagg ttcgattgat agttgtctgt
    13441 gaaacacctt gttttgttgt attagcattc agcaagggac gttcacgtag ataaaggata
    13501 aaggattcca tgtctttctt agacatattt tccaaaactg ataaaggaat atcggatatt
    13561 ttatcagcgt ttgaaatacc agactccaaa acccagctga aaaatcggtc atattcctta
    13621 aggtattcgt acagggttgt aaaactgtag ggcacagcca gcttagattg gtagtattcc
    13681 agaacatacc agggcatgag ttgttttagt ttgtcgattc gttccagtaa aatctcacgt
    13741 ttcatgtatt ttctccataa gtaagtatac tagtagtata gcatagactt gtatattttt
    13801 caagaaaatt atagtttttc agaaagatgt tataggagtt tttcaaagtg ataaaaagac
    13861 ttagacctac aaacaaaaca gaataaggaa aataataaaa atctcaccca ctaaacttct
    13921 gttaatacta cataactgaa gagctatata aggagctatg agatacaaaa cacattgtat
    13981 aatagattga aactagaata gtacatctgg acttctaaaa cattgctaga aattgatttg
    14041 attgttctga ttgttttatc ctatttctat ttcatttcac tatataattg ctattgtttt
    14101 ctttttcatg attttaatct tactgtacag atttgataca gtaaactcct ttctagcttt
    14161 atactttatc atttctaact taattatagt cttattttta ataaagttca attatttata
    14221 tgtaaaattc ctcatcagta aaaaataacc gatctcattt ctgagaatcg gttttctaaa
    14281 taaatcaagc tgagtattta cataacataa attatgtaaa tactcatact acaacaacaa
    14341 atctttgact tttccaattt cactttttgg aataaccagg tgaatcatat cacccagata
    14401 cattctggtt gagccgttaa ctgtttggct cttgccatta tggacttgag ttgtgatgag
    14461 gacgttgtgt ggtaagttga gttcatgaac ttgtttccca gcaattttat cagaaactgg
    14521 tatttcgata agtgtaactt ctccttcgct agatacttct tctggaagca ttttttccag
    14581 catggcttca tagactggcg tacctttgag caaatccatg ataatataag aaacaagagt
    14641 gacaagacca agtggcatga ggttgcgaat atctcctacc atctcagtta cgaggatcat
    14701 agcggttaag ggagcttttg atatggctcc aaaatagcca ctcattccta gaatgacaaa
    14761 tatagggaat tgctcttgac tgacaagtcc aagattgaca cagataacac caactaagga
    14821 accaagcaaa gaaccaagag ctaaaatggg gaggaaaatt cctcctggca gtccacttcc
    14881 atagctaatc atactccaaa taaagcggat caaaaagtaa gctaataaaa cttggaaact
    14941 aaaattttgt tcagttaaag aaaggacaag ctgatttccg ccaccaatga tttgaggtaa
    15001 gaagattccg actggtatga taatgataaa agccaagatg ggataataag ccctatccaa
    15061 atggattttt tgaccaatca agtcataaac tcttccaacg tttaatacag ctttctcata
    15121 gagaaaacct gaaaatccaa ggaaaattcc catgacgaga tatatccaat actggtctag
    15181 ggtcatggga ggaatgttat ctggcatatc caatactggt gtcaaaccga acatgagtag
    15241 agacacaaag tttgctacga tgctggctgc tagagttgag acccagaaaa agcgcgaaaa
    15301 atggtgatag acttcttcta caacaaagag aagtgctgca ataggagcat taaaggctgc
    15361 ggctaaacct gctgcagctc cactggcaat caaggaacgt tcctctactg gactggattt
    15421 gagccacttg gcaattcctt taccaccaac tgctccaagt tgaatgctgg gtccctctcg
    15481 acccagcatg agtccactgg caatagcaag aatacctagc acatattttt tccaaagaat
    15541 gccccaccag ttgagggaca tgaggccttt cagttcggct tcgacttgag gaattcctga
    15601 gcctttaata tctttttctg accgtgttag tttggcactg agccaacaga tgagtatata
    15661 aaacaaaacc agtacaaaaa gattgcgcac taagtaccct tgatcttgat aaactccttg
    15721 tatcaggtgg aagccctttt caattaagaa acgaaaggat ccgacgataa ttccaacgat
    15781 gaggccgaca atgattcctc gaccaacttg ggatagtata gtgctggatg caaaggcgaa
    15841 ttctttctta gaacggacta tttccgactg ttcctccatg gtatttcctt tcaattaact
    15901 ttctttttta cctaaaacca gtgatttaac gggttcaaag ctggttcggt gaattggggt
    15961 aactcctagt tttgtgaggc cttccagatg tttagctgtg ccatatcctg cattagtagc
    16021 gaaatcatag ccagggaact gctgatcgta ttccttcatc aattcatcac gtgttacctt
    16081 ggctactata gatgctgctg cgatagagag ggagttggca tctcctttga taatggaggt
    16141 ttgtgaaatg ggcaagtcca gtttcatggc atcaatcaaa aggtgctctg gttgagggct
    16201 gagctgggag attgcttctt gcatggctag tttggttgct tcatagatat tgacttggtc
    16261 gatgacctga ttatctatga taccaattcc aatcgacaag gcttggtctt gaacgacttg
    16321 gaaaatctcc agatgtttct ttttaggaat tttcttgctg tcgttgagac ctttaatctt
    16381 acaattttta ggtaaaataa cggccgcagc gactacagga ccagcaagag gaccacgacc
    16441 aacctcatca atacctgcaa ttaaggtcaa tccttgctta taaagttctt tttcataaga
    16501 aagcatggat tccaagcgca aattttcatc taattcagct tgaatggctc ttttatgctt
    16561 gctgatttcc ttttgaactc ctgagcgatt atccttttca agatctaaaa aaatagggct
    16621 ttctaactcc ttgactgtca caaggagttc tttgatttct ttaatcgttg ccatcgaggt
    16681 cttccaatgt atctaaggta tagttaccga gtttgccatc acggacttcc ttcacgaaga
    16741 gactgtaaaa acggtcatag tcatcacgga aaccgagggc gcgggtcata tccataataa
    16801 tcacaggcgc ttcttcttca attttcattt gtttgaagcg ttcagccagc ttttctggat
    16861 aatgttcttt gaaataattg ataccaaaaa tggtaacctc atccatagga agcaactgat
    16921 ccttgatagc tccagtcaat gccaacttaa gtgcaacagt ttcatcctca aacttaggcc
    16981 agagaatccc cggtgtatcc aagatttcca gatctttatt ggttttaagc cattgttgac
    17041 cttttgtgac ccctggcttg tttccaacaa cagcaatctt tttaccagcc aaacggttca
    17101 tcagagttga tttaccagcg tttggaatcc caataatcat ggtacgcaag gtttcaatct
    17161 gaatcccacg ttctttctgg cgagcaatct tatcagccat gagcttcttg gccgcatctg
    17221 ttacaacttt tacagtcact tgctctttgg agttgatagc tagcgtctgg attccttgtg
    17281 attcaaaata ctgacgccat tccttggtca ttgctggatc agccaagtcg gccttgttta
    17341 aaatcaagag ttttggttta tcaccaacaa tcttggttaa cataggattt tgactagata
    17401 gaggtaagcg tgcatcaact aaaatcgtca caaaatcaac aaattttaaa ttctcctgca
    17461 cctgtcgacg agctttagac atgtgaccag gaaaccattg aatagtagcc attgagtttt
    17521 ttcctttcat agcaaggatt tagatcccct attttatttt actattgttt gaacatagag
    17581 agttttcaaa tgaatgatgt ccaaacagtt cttgttttat gacatagaaa tctactttat
    17641 attatatcat gccttcttta ttttgtccga tgatagcctt ttaccctata tagaaaaaag
    17701 ccatcaaatg atgacttttc agagggagca aaaaactgtt cccgtcatat tatgaaccaa
    17761 aagaactaaa ctaccaacag ctaataggcc taaagctgta agtgcagaag ttgatgaacc
    17821 tgttgcagga agagtttcct gctgataaat aagagcataa cttacttcat tatcagtagg
    17881 aattcctgct tgaatcttgg tttgttcttc atcacctttt taaactttat ttcctaatag
    17941 taaacactat tttttattct caaaaattga acttctaaaa tcatttgttt ggtcaagata
    18001 ggctttaaag attgctctct tcaacttatg aacggcactg tcgatttctg gatcataatt
    18061 actccaacgt ggaccttcag catccttgag aacagcttgg tccatgaggg cttgcaattc
    18121 atccacatta ttgatagtct ttgtgccata gcttggccat ggttttgtcg gatctttaaa
    18181 ggtcacctgc ttcaagtttc caaactgatc cactcgttct tggtacatag ctgtcttaaa
    18241 ttctgcccaa gttttatact taccgtcaaa tacctttttc aaaacaagct catcggtcac
    18301 caagccccgt tctttaccat aaagattgat agtttgccct tgttgtttag catcttcttc
    18361 gtattggttt gagatataag gtaccattcc atccttaaag cctttggcag ccaaaagttc
    18421 gtacgcaatc ctacgtccca taaggtcccc tggtgtgcct ttctcactgc tgagagctga
    18481 atagattggg gcaaagagtt tgatggtata gtagccattt cgttcatagt cgccagactg
    18541 gtactcacga gctgataaga tgttgtggtc aatcaaactt tcaaaactat tcaggtttcg
    18601 ggcctctgct tctgtcaatt ctttgactac gttagtggca taaacctcat ttccatctgc
    18661 tggatctttc acatacttgt tctcaatttt tctgagagcc gccattttct ggtaaacact
    18721 tagtttatta acgattgact gcccttcgag atattccatc atgtagataa ggtcaaacat
    18781 gttatggaca tagttctgaa gatctgctgc gttttggaat ctctctgtcg gatccaagac
    18841 ttgaagacgg gatccctctg tactatctga ttttgagtgt ttcaagatag aattaatggt
    18901 aatggttgcg tcacttggtt ggtcaggggc ttgcaataag ccttttgcga agaactctgg
    18961 tcccaagcca tttcttcgac catagccacc aaggtaaata tcctgatccg aatcatgggt
    19021 catttcatgg gtataagtaa tggctccatc cttgtccagc atgcggtaag acatatagta
    19081 gacgccgtcc cctgtagcgt aggctccatg ctggttgtgt actaccttat tgccaactgg
    19141 tccaaagaag ttcttcatag ctggattgga gctatcaaac ttagcctccg ctgtagcttt
    19201 tcctgatgtt gtgtcatccc caaacttata ggcatcataa aggagaatcg tacgatagag
    19261 tttttcgcgt gactgttcat ctaaaatacg ataccaataa tcgtagtgat ctcgctgacg
    19321 tttggctgtt tcacgcgcat tttcttcaac aaaatcattg agagccttgc ccgctttatg
    19381 gtcactactg cggtagcgat cataagctcc aaatcctaga ctagacatgg tcgagatgac
    19441 aaatacggat ctctctggca aggtcaggag aggcaagacc atattgcggt atttccatgt
    19501 ggcactcgtg atacgatcat aaacaccgat agaatacttg gtgccagcta acccttgctt
    19561 cgttttcacc tcttcgatag tggatttttc ttcgacaatg taagccttag tctctgattt
    19621 aaaccagtca ttattgcttg tatttggtaa aaagactttt cggtaatgtt ccagcgtgct
    19681 aaacaaatct gtcgttccat gttgactggc aagactgata ccataagtat cgacattatt
    19741 cttagctaga agattgttaa agccagattt acctaactca atcagagtat ctagtgggga
    19801 agtatttcct ttaccaaaga agtctggatg atacataact aggtctttga cattcacttg
    19861 accatagtta aagttatacc aacgttctaa ataagttaaa ccgagtagca aggcttcctt
    19921 attattcttg attttatcta caagatagcc tctggtgact gagttatcgc tagctagacc
    19981 ggcatctgct gacaaaagtt ttttcaagct gtctcccaga ttttgttttg ttttggagaa
    20041 ctggtcttct aaatagagct cggttaactt aacttctgga gaaataccaa gtgtctttct
    20101 gatagcatct gactggtaat caagcttctg caactcaggc aagacttcct taacaataga
    20161 gtctcgatca tataagaatt ggttaggagt gtagagaagt cccgtattac ctagattata
    20221 ttctgctaga ttggcaaaat cagtctggta cttgagatca aatttctcag atgaatggtc
    20281 attatagtga agtaagagtt tgtttgcagt ctgtttgttg gaaacaatat ctgtgattac
    20341 ttggtcatcc ttcatcataa ctgcagataa gagttctttt tggtataaga gactgttctc
    20401 cttaaccagg tttccgtatt tgacgatggt tgacttatta taaaaaggta acaatttttc
    20461 aatgttctta taaaccaaag cacggttagc ttgataatgg gctatcttag aaaaatcact
    20521 gtctttcttg ccacttgttg agaggggttc caccgttggt agagtgagag gatttatttc
    20581 tgcttttttg cttacaattt gagaagcatc tagcattgtt cctctttctt caaaggattc
    20641 cttgctgacg acctcatcct tctctaaagt gacattgaaa actctattag ccttactact
    20701 aaatgtatta gctaccttca ttcctgtata gtggtatcca gtgatggcat ttccgtttgt
    20761 tacattgaca tcgctaagaa cattggtcaa ttcaccagcg tgtttttcct caccagaagt
    20821 tcgatcccac aaatatcctg ccacaccagc gactttacca aagtgcttga cattattaat
    20881 atcaccttcc gcataactgt tctgtatatg cgcatcttgg tctactagac ctgcaagacc
    20941 accgacagtc tgatctgacc tattggtgtt tgacgaaatg gttactgtcg ctttggattt
    21001 agcaatagac gcattttttc ctgttaaatg accgaccaga ccgccaatat tgtaggcatc
    21061 agtcgtttca taggtattga caattcttcc cttgaaactg ctctctgcga tgcttgattg
    21121 gtctgcctta gccaacaaac caccgacacc acgttctcca gccagaacac catcaacatg
    21181 aacttgttta atctttgtgc cattcgtagc ttcatttgct agtgaaccaa tatcattttt
    21241 ccctgaaata gcaacatttt ttagactcag tttttctact gtagcaccac tcaagttttc
    21301 aaacagaggt tttttcaaat tatagatagc ataattcttg ccatcttttt caccgattaa
    21361 acgaccagta aaggtgtcct tgatatagga tctttcatca ggaccaagct ccacttcgtt
    21421 agcattcagg ctggccgcta aatgataggt tccagaggga ttttggttta tagctttgac
    21481 caggttgcta aaggaagtaa agtttgtatt ttcctcttta gccttcttat cgaggtagaa
    21541 ggtgaaattg tcgacatatt tattttctag ttcttgctgg agtttctcag ctttggctgt
    21601 gattttataa actgactgcc catttctttt ctcttcagtt attgaagcta ctggtatata
    21661 gacatcttta aatgctgaag attttacctt gacaaagtaa gtatccgtat tctctggaat
    21721 tccatccaat gaaacatgtt gtctgtaggt tccattcgtc tgactataca actcaatatc
    21781 agaaacattt cttaattcga gttttttctc tggttctgtt tgtccattct ctgatgttgt
    21841 attacctgaa ttttctggtt ttgttgcagt tccgtttttt tctggttgat ttgattcttc
    21901 aactggtggt tttgaatcac taggtttatt ggatacttct ccagtatttt cgttagctat
    21961 tttcccagag tttgtttgtg tttcttctgc aggttgaact ggtttttctg tttcttgatt
    22021 tgaggtacct tctactgtgc cttcatttgg atttactgga acttcttcta cagttttttc
    22081 tgaattttca tttttagagt cattatgttc tggtttattt gattctccaa ctgaggttgt
    22141 cgaatcacta ggattactgg acacttcccc agtattttcg ctagatgtat ctggtgatac
    22201 tttctctgaa ttcgttgttg attcttctgc aggttgaact ggattttctg cttcttgaat
    22261 tgaggttcct tctgtagtac cttcatttgg atttactggt gtttcttctg ttggttttac
    22321 tggaacttct tcagtttttt ctggaccttg ttctttggtc ttctcaaccg gagtttcagg
    22381 ttttacttgc tcaatattac ccttatattc tggcaatggg gctacctgct ctggttcacc
    22441 tttatcactt accacagtat ctggcgactc tggttgaacc tcagtctcac ctttgtcggt
    22501 cacaactgct tcgggtaatg tcggttgaac ttctggttcg cctttatcac ttactacagc
    22561 ttcgggcaac tcaggctgaa ttgcgggttc aacaatagat ccagactgta cgtccttatg
    22621 ttctacacca gtctcaggtt gttcctttat aacttgagtt tttttagtac ctttttcgac
    22681 tattcttgga ctaggcgcag tcgttgaagt tgaaacaatt tctcgcgaaa cttcttcctt
    22741 gtttacagag aatattctga cgatttcaac tttcttacct aatttacctt cttgttttac
    22801 tcttacagtt ccttcagcta aatcaggatt ttcttgaatt tcttcttgaa aatctatttt
    22861 tgtctctata gtttcctcac gatataagag ttcaggtttg ttcaattgac ctgataaaac
    22921 ttcatcctgt ggatttaatg tatttacccc agtcttttct tttggagaaa tcttctcctc
    22981 tttcttcgtt tctagattct tatgttcggc taattgttct tgagaatctg aagattgttt
    23041 ctcttctttt cttggattga ttaattcagt agagaaaggt ttttcaacta cttgaacttc
    23101 tgtcggctta gttgaagaaa caggtgtttg ttcctgaata gcttgtactg ttgatggatg
    23161 gtctacaaaa tttggtgtaa cattataatc caccttttgt tgttttgtag gagtggctgc
    23221 tgatttttct tgattactta cttcaaaatc agaagtcgtt tttccctctt tgatatatcc
    23281 aatataagtg taacctgaaa tctctttagg aagaggtaat ttttctccag aggtcaattc
    23341 atagtccgta ttgtaattta gcaaaagatg attttctaaa gcatggactg aaactaagac
    23401 accatttccc attcccgcaa ccaatactaa atgtaatacc gttttattct taaccttttt
    23461 cttggaaaca gcaaaaatta aaattcccag agcagctaag ctagcaccag caactagggc
    23521 ttgcctctca ttcttgcttc cagtatttgg taattccgcc agttgatttt gagaatttaa
    23581 cttataaaca agataataag tttcatcatc attctccacg tatgtcggaa tatcatagac
    23641 aagctgcttc ttttcttctg atgatagctc tgaatctgcc acatatttat agtgaactcc
    23701 cgcagtttct tgagcatcca cagatgaact agctaataca gacataaaaa ataaacttga
    23761 aatcgttgca gatacaagtc ctactgataa ttttctaaat gaaaaacgct cttgtttttc
    23821 accaaaatac ttttccatta ttcctccttg aaataaaatt tatatatgtt acaaagacct
    23881 ttattatatt agtgtattat ctattatcta tagaaaaggc agtatacctt aattatactc
    23941 ttaatttaca aaaaagtctt aaaattgaga tgcgctttca tactttgttt tatattattt
    24001 gggggtacaa taacacctac catgaaattt acacggtagg tgttactcat atcactaatc
    24061 gttctaaaaa tggtttgagg cagttgagga gaattccttc tatccagctt ccttgtgctg
    24121 atgagcgatg gtcttcctgc aggctttttt ttagaaaatc tcggacttgt tctggtgcga
    24181 tttcaaattc aaaggctttc attttataga aaaagtcgat gagatgatct gacaggtatt
    24241 cagttgaaaa gggtacttca ccactttttc gatattctaa taagagtcta gaaaatcgag
    24301 ctttttcttc aggaagctca cgaaaatagg aattgaggat ccaagtctgc ttctgttttc
    24361 tttcaattgg atcctgactg gcaattcgtt ggtctttttc cagctctttt tggtattgtt
    24421 tggccttgat agctcgttct gctctatttt taccaaaaag aattttttcc cacttgcgtt
    24481 cttcttgagt cagggtctct gtaaagccaa agtaatcttg ataagcacgc tctgcgggtc
    24541 ccatggctag aaccagattg tctgcatatt gcttggcgat tttatccctc ttcttgcgtt
    24601 ctttctctgc ctggatacgg agttcttgtt cgtagtcaat tttctccttg cctagcttga
    24661 caaggtagag ttggtcatcc gatttcccaa gtaaaaaggg tttgatacac ttttcaagga
    24721 cttcttccat ccgagccttt ttctttggtt ccgccttggt ccaacttcct ccctgaaaga
    24781 cttctaggaa aagctggtag tctctctcag gcgcaaattg attgccacga ttgggtttga
    24841 aaacaccttt ttcccagagc cattttagaa gtcgctcgtc aaagtcactt ttattgacct
    24901 tgattttttc ctttttctga gcttttctgg ttagattttc aacctttctg agcagttttt
    24961 cttcctcttc caattgctgg tcaaggggca atcgatgaaa atgacgaaca cagtcgctac
    25021 caattggaaa gaggcgttgg cctgtgacac cgttaaagag ttcataagcg tatttgatgg
    25081 catttcgaca gacacaattg ctacggccaa taccgttaaa aatcaaggaa acttcattcc
    25141 attccttggt agcttgttcc caagtatccg ctttcgaagc ctgtaaaact gcatcgtgca
    25201 gggattttct aactggaagt gtcatgaggt ctcctttcta atactcaata aaaatcaaag
    25261 agcaaactag aaagctagcc gcagtcagct caaaacactg ttttgaggtt gt
//
LOCUS       DAVBCG010000017        22375 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBCG010000017 DAVBCG010000000
VERSION     DAVBCG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22375
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.017"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(83..1063)
                     /locus_tag="VQ495_000580"
     CDS             complement(83..1063)
                     /locus_tag="VQ495_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2530574.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1379..3394)
                     /gene="recG"
                     /locus_tag="VQ495_000581"
     CDS             complement(1379..3394)
                     /gene="recG"
                     /locus_tag="VQ495_000581"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2530575.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     IKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3413..4516)
                     /locus_tag="VQ495_000582"
     CDS             complement(3413..4516)
                     /locus_tag="VQ495_000582"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2530576.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4506..4874)
                     /locus_tag="VQ495_000583"
     CDS             complement(4506..4874)
                     /locus_tag="VQ495_000583"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2530577.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(4913..5944)
                     /locus_tag="VQ495_000584"
     CDS             complement(4913..5944)
                     /locus_tag="VQ495_000584"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2530578.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(5946..6977)
                     /locus_tag="VQ495_000585"
     CDS             complement(5946..6977)
                     /locus_tag="VQ495_000585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2530579.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7058..9571)
                     /gene="secA"
                     /locus_tag="VQ495_000586"
     CDS             complement(7058..9571)
                     /gene="secA"
                     /locus_tag="VQ495_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2530580.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9717..10004)
                     /locus_tag="VQ495_000587"
     CDS             complement(9717..10004)
                     /locus_tag="VQ495_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530581.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10015..10665)
                     /locus_tag="VQ495_000588"
     CDS             complement(10015..10665)
                     /locus_tag="VQ495_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2530582.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(10669..11310)
                     /locus_tag="VQ495_000589"
     CDS             complement(10669..11310)
                     /locus_tag="VQ495_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530583.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            complement(11303..12094)
                     /locus_tag="VQ495_000590"
     CDS             complement(11303..12094)
                     /locus_tag="VQ495_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530584.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(12279..12614)
                     /locus_tag="VQ495_000591"
     CDS             complement(12279..12614)
                     /locus_tag="VQ495_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530585.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(12754..13089)
                     /locus_tag="VQ495_000592"
     CDS             complement(12754..13089)
                     /locus_tag="VQ495_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530586.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13180..14490)
                     /gene="der"
                     /locus_tag="VQ495_000593"
     CDS             complement(13180..14490)
                     /gene="der"
                     /locus_tag="VQ495_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2530587.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14504..15217)
                     /locus_tag="VQ495_000594"
     CDS             complement(14504..15217)
                     /locus_tag="VQ495_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2530588.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15214..16110)
                     /gene="dnaI"
                     /locus_tag="VQ495_000595"
     CDS             complement(15214..16110)
                     /gene="dnaI"
                     /locus_tag="VQ495_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2530589.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16111..17280)
                     /locus_tag="VQ495_000596"
     CDS             complement(16111..17280)
                     /locus_tag="VQ495_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2530590.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17281..17754)
                     /gene="nrdR"
                     /locus_tag="VQ495_000597"
     CDS             complement(17281..17754)
                     /gene="nrdR"
                     /locus_tag="VQ495_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2530591.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            17894..18259
                     /locus_tag="VQ495_000598"
     CDS             17894..18259
                     /locus_tag="VQ495_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2530592.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            18264..18959
                     /locus_tag="VQ495_000599"
     CDS             18264..18959
                     /locus_tag="VQ495_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530593.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            18976..19743
                     /locus_tag="VQ495_000600"
     CDS             18976..19743
                     /locus_tag="VQ495_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530594.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYTGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20096..21295)
                     /locus_tag="VQ495_000601"
     CDS             complement(20096..21295)
                     /locus_tag="VQ495_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2530595.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21292..22185)
                     /locus_tag="VQ495_000602"
     CDS             complement(21292..22185)
                     /locus_tag="VQ495_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530596.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
BASE COUNT         6543 a         5057 c         4171 g         6604 t
ORIGIN      
        1 aacgcatagt atcaagtttt tttaatcccc tgatactatg cgtgtttgta atttttaaga
       61 ttttgtgctt agagtcgact ccttatttta gatatttaaa aggaatctca cttccacaga
      121 gccagttgta gacttggtca ttgacaaata cattcatggc ttcgtgagca tactcaggca
      181 tgatgcgata ggttttatcg caggtcagac gattataaat cgcaaactgg gtaatgggat
      241 agcaaacatc gtcgtccaag cccgtaatca tcttaacctc accttggata cgatgggcaa
      301 gatttttgac atcgatatag gcaagggtcg ccatgatttc ctcctctgtt tcatggaagg
      361 ggtcgtgaaa cttgaaataa cggaaaagtt cgtcgtaagc ctcgctagta ttaccaatct
      421 caatcacccg tctgaagtct gacaagaagg gataaatggc aactgttttc tgaattcgag
      481 gattgagcgc tgctgcaact agagctagag cccctccttg tgaggcacca tagctagaaa
      541 gacgcttctc atcaacctga gacagactag caacaatttc gaccaactgg taaatatcca
      601 gataaacatc cttataaaag aggtggtccc gaccttccat agcaccacgg ataatatgcc
      661 ccttcacggt atttcctaaa ggagaacgca agccgtcttg tgagtaacct gactggcccc
      721 gcacatccat ggaaacaaca ccgtaaccag ctacggtgaa gcccagcatg tcggcccagt
      781 cccagccacg tcccatataa ccatgaaaat ggaagattag tggaaccttc tcctcactct
      841 ttggaagaac aatgcgtgca tagacctttc cttccttgct tccttcaaat gttaactcat
      901 agcacttgac ttgaggaatg tggaaatctc tttcctccaa gtggtaggat ggaagcgtgg
      961 aaacattttt cacttcccca tcccagaaag catcaaagtc ttccggaacc tcatcccttc
     1021 ctctataggt cttaatttct tctagcaaag ctggattttt catagcatgc tccttttatt
     1081 ttgtaatcgt taccacaact gtagcatatc tatgtaatca atgcaagaaa gaaggataaa
     1141 tagaaagtga atttagattc tttactttta ggatgatggg ctgaaagtag ttttagaaac
     1201 gaaaaagagc atttcgctct ctcttctgtt tatactagtt ttttgcttcc ttcttttgga
     1261 aagtggtttg gtaatttact ttaatcgtta ctgtcatatg agagtcctcg tttctttttg
     1321 atgactttag tataagagcc aaacctaaaa actagcttaa gttttcctta gagaaagctt
     1381 aatccagatg ttctttcttt tccagatgaa gggcaatcat gcgccactct ggatcttctt
     1441 gccaagcttc tatagaacta atgtagctag caacctttct tgcttcttct aaaatcggaa
     1501 aatcttcgat aatatcagcc acttggaact ctggaagtcc tgactgtctg gttccaaaaa
     1561 tctcaccaga accacgcatt ttcaaatctt cctccgcaag gacaaatcca ttggtcgttt
     1621 ctgtcatgat acgcatgcgg tctttcccag aatccgtctt gggattggca acgagaacag
     1681 cgtaggactg cttgtccccc cgaccgacac gacctctaag ctggtgaagt tgactgagac
     1741 cgaagcgatc ggcatccatg ataatcatga cagtcgcatt gggaacgttg accccaacct
     1801 caataaccgt cgtcgaaacc agaatatccg tctttctctc cttgaaatcc tgcatgatct
     1861 ggtctttttc gtcactcttc atcctaccat gtagaagagc cacctctgcc ttgcctgcaa
     1921 aatgagtcgt caactcctct gataaggcaa tggcattttt caaatctaga gcttctgatt
     1981 cttcaatcaa aggagagatg acataggctt gggaaccttt ttgaatttcc ccctctaacc
     2041 aagtcaagac ctgaggtagt tgctcatgtt tgatccagcg cgtcacaata ggcttccgac
     2101 ctgctggcat ctggtcgata atggaaacat ccatatctcc aaaggctgtg atggcaagcg
     2161 tccgtggaat gggagtcgcc gtcatcatga ggacatctgg attgtcacct ttttcccgta
     2221 aaatacgcct ttgccctaca ccaaaacggt gctgctcatc gataataatc aaaccaagac
     2281 gagcatactc caccccatct tgtatcagag cgtgagttcc gataatcaaa tcagcctcac
     2341 ccttggcaat ggtctccaag acctctctct tttctgcagc tttcaaggaa cctgtcaaga
     2401 gagccagttt caaattggga aaaaggttct gtaaactctc aaagtgttgc tctgcgagga
     2461 tttctgttgg taccattagg gcagcctgat aacctgctgt cactgccgca aacatggcca
     2521 agccagcgac taccgttttt ccactcccca catccccttg taggagacga ttcatgtggt
     2581 ggtcggactt catatcagtt aaaatttcct gcaaactctt ttcctgagct tgggtcaggg
     2641 caaaaggaag acttacttta actgctgtca ctttttcctg agaccaattc agaaccagac
     2701 cacttccctg aactctattt tcagacttga gcatctgcag ctgcatttgg aaataaaaga
     2761 gttcctcaaa cttgatacgg cgaagagcct gcttgtattc tgccaaatcc tttggaaaat
     2821 gcatagctcg gactgcctga caacgggaca tgagtttgta tttgtctagt aaagactggg
     2881 gcagattttc ttctatcaag aggtccagtc cctgatcaaa agccgtcttg atgaccttaa
     2941 ccagactggc ctgactgatt ccctgagcca gacgatagac aggctggagg tcatcttcta
     3001 cctgagccag aaccttcatc ccagtcagac tagccttagc gcggtcccat tttccaaaga
     3061 cagcaagggt tgctcccaac tctattttat cagccagata gggctggtta aagaaattca
     3121 ccgcaaaaac gacctctccc tgcttgagac taaaacgcag gcgattgcgc ttgaaaccat
     3181 aatactggac actagcagga gtcactacct gaccagaaag aactgccttc tcaccgtctt
     3241 ccagctccag cacctgcttg attttgaagt cttcataacg gaaaggaaag tagagcaaga
     3301 gatcttgcaa gttttcaatt cctagtttgg cgtatttttc tgctgacttt ggtcccacac
     3361 caggcaagac atgcaagggt tgatgtagat tcatgctcca ctcctttctt ttctagtaat
     3421 attctctcgg aatacggtcg ctgaggaggc aaaccacctc atagttaatg gttacgcggt
     3481 aggtcgctac ctgagttgca gtgatttcct tatccccatt ggagccaatc aaggttacct
     3541 tggttccgag tgggtaaggc ttaggcaatc gaatagtgat ttggtccatc gaaaccctgc
     3601 cgacaattgg gcaagcttgg ccatctacca agacagagaa attttgcatg tctcttgtcc
     3661 atccatctgc atacccgatt ggcacggtcg cgatgacttg ctcgctatcc gtttgataag
     3721 ttgctccata gcccatgcaa gcaccagctg gaactgtctt gatatgaacc agagcagact
     3781 ccaaggtcaa ggccggtatc aaatcataag gcaaagccaa gaccgctcca cttggattga
     3841 gaccatacat ggcatctccc atacgaaccg cattgaaaat agtctctaca tgccaaagag
     3901 tcgttgcaga attgctagca tgaaccagct ctggaacttc cttcatacta gctaaaatag
     3961 ttttaaaccg ttctaactgg gcattaaaat agtcatctga ttcctcatca gcagtagcaa
     4021 agtgggtaaa gattccttca acacgaacac cgtgttgttg gagcaaatct tgagcctgct
     4081 caacctcact tgcctctcta aaaccaatcc gtcccatccc tgaatcaatc ttgaggtgga
     4141 ctgtcaatcc agttaggtcc acttccttat ctaagagtgc ttgaatccac tccagtccag
     4201 ccactgtcaa ggtgaagtca tattctttag ctagagcaac agcttcgatt tcagaaactc
     4261 ctaaaatgag gattggcttg ctgagtccag cttgtctgag ttcaatggct tcatcgatat
     4321 tggaaacgca aaagccatca acatcatctt gaattgcctt ggcaacggca acagctccat
     4381 gaccataagc attggcctta accacagcca acttgagcgt tccttgaggg atatgagccc
     4441 ccatttgctg aatattttgt cgaatagctc ccagatgaat cagagccttg gttggtctat
     4501 gtggactagc tttcatgatt ttcctccaaa atgacactgg ctgtcacaaa ctgatcggtg
     4561 tggctgatag acagccaaat ctttcctgaa aatggtgcct gactaaaata aggcgcccca
     4621 cgttcattgt tcaatacttc caaatcctga aaaccgagct tgctaatgcc cgttcccata
     4681 gccttggaaa aggcctcctt agccgaccag cgaccagcta aatattctat ttgcctgcgt
     4741 cctttgagac tggtgaagcg ctccatttcc tgagcggtca gtacacgctt agcaaatcct
     4801 tcatgtcgtg taactgcgct ttctatcgaa gccaattctt cgatgtcaat tccgtgtcca
     4861 actatcattc tcatcaaaag gagttgagat tccccaactc catccttttc acttattttg
     4921 tcaaggtaac ataaatctct tctaccaagg cctctgtatt ttcccaacct aggcaagggt
     4981 cagtaataga gcaaccaaag atctctggtt ggttttgacg accatctgct aggtaagatt
     5041 caatcataaa tcctcgaacc gtctttttaa ttttctcatt ccaatcacga ttctgcaagg
     5101 tctggcgaac aattcgaatc tgctccatat attgcttgcc tgagttatca tggttggtgt
     5161 caatgaggat aaaaggattt tcaagtccca tggtttcata gcgttcaatg gcttggagta
     5221 ggttttcata gtagtaattc ggcatataat tgccatactc gttgactgct ccacggagga
     5281 taacatgggc caaaggatta cctgatgtct caacttcctg cccatgataa aggaaggttt
     5341 gcttgttttg agcagcatag atggcgttaa acataacacc caaatttcct gaggttggat
     5401 ttttcatccc tactggtgca tcaatcccag aagccacaaa gcggtgctct tggtcttcca
     5461 cagaacgagc tccaacggca tggtagctga ccaagtcatc caccaagatc agatttgacg
     5521 gataaagcat ctcatctgcc gttgtcaaac cagtctctgt aatcacgcgg taatgcaact
     5581 ggcgcacagc ctgcaaaccg ttaatcaggc ttggagcctt agaagtatct ggctggtgaa
     5641 ccaatccttt atagccgtct ccattggtac gaggcttagc agtatacacg cgcatgacca
     5701 tgaaaatctt gtccgctacc ttcttttgca aggcggataa acggcgagca tattccaaga
     5761 ccgcctcttc attatcagaa gagcaaggac caatcaccaa gagaatacgg tcatcttccc
     5821 ctgaaataat atctgccaat tccctgtcac ggctttcctt gagtctcaag gcttccgcag
     5881 acaattgggt ttcagccttg atgacttcca tatcgatttc ttgacctttt tcaataaatg
     5941 ccatcttatt ctcctaacgt tttgtaaatt tctctgacaa gagcttctgt gttatcccaa
     6001 cccaggcaag ggtctgtgat agacttgcca aatacttctg gctcattttg tcgaccatct
     6061 tccagataag actcaatcat aaaaccacga acgaactgct taattttttc attccaagca
     6121 cggttaatca aggtctggcg gacaattcgg atctgttcaa tatactgctt accagaattg
     6181 tcatgattgg tatcaatgat gataaaagga ttttccaagc ccattttctc atattgggca
     6241 atggtatcaa ttaaattgtc ataatagtag ttgggaatat tttttccata ctcattaaga
     6301 gcaccacgaa gaatagcgtg tgaaagcggg ttcccagttg tttctacttc ttttcctaag
     6361 aaaaggaaac tttgtttgtt ttgagcagca taaatcccat taaacatgac attgagattt
     6421 ccagaggttg gatttttaaa accagtagaa aatcctgccc cacttgccac aaagcggtgt
     6481 tgctggtctt caactgaacg ggcaccaact gccatgtaag aaatcaaatc atctacaagc
     6541 ggaaggtttt caggataaag catttcatca gcagttgtca tccctgtttc tgtgatgaca
     6601 cgatagtgaa gatggcgaac ggctttgatt ccattgataa gactaggcgc ttctgtcgcg
     6661 ttaggctggt gaatcaagcc cttatagcca tctccgttgg tacggggttt ggcagtataa
     6721 acacgcataa ccataaagat acgatctgcc acttcttctt gtaggactgc caaacgctta
     6781 gcgtattcaa ggacagcttc ttcgttgtca gatgagcatg gcccgattac caagagaatt
     6841 cgctgatctt ctccacgtat aatggcttct agctcttgat cgcgctgtga tttcctctcc
     6901 aaagcctgac cttctaattt tgacaaggca cgaacttctt ctatatttat tttagagctt
     6961 tttgctgtaa ataccataac tcatctcctt ataaagcaaa cggataccca gtaaaaattt
     7021 acttttcaca agatatccgc ctctaaacta tctcatttta ttgtctttta ccgtgacagt
     7081 ttttaaattt cttaccagaa ccacatgggc aaagttcatt gcgtccaatc tggctcaaat
     7141 ccaaatcttc tggcatactt gcttggtgag cagcgatatt gcgagtcgct gttgtactga
     7201 tatgacgttc tgcctgtggt ctttcttgtt catgaatttg tgctttcatc atcaagcgtg
     7261 tcacatcaaa ctcaatcgaa ccaatcatat cattaaacat acggaaacct tctgcctgat
     7321 actcaacaac agggttgttc tgagcatagc cacgaagtcc aaccgcgtta cgcaattgat
     7381 caagggcatc gatatgatct gtccacttgt tatccaccac tcgtagaatc aaaacttttt
     7441 ggaattcttt aactgcttct tcatcgcgta gttttgaaac ctgactatcg taaaccttca
     7501 aggcacgttg gaaaagctct tccttgatgg ccttatcaga caagcctgac aagtcttcca
     7561 tcgtaataga atcttcagga agcaagttgt acttagcaaa gttcaaaatt gcctctagtt
     7621 tttcatcttg tttggcacgc gcatgaccat caacgacacg ttcaatcgtg cgtttgatca
     7681 tagcctgaat ttcaggtgcc aagtcacgat ctgcagtgat gacatcgtaa cgttgagcat
     7741 agataatctc acgttgttca cgcatgacat catcgtattg aaggacttgt ttacgggtat
     7801 cgtagttatt tccttcgaca cgtttctgag ctgcttcaac ctgacgcgtc aacatgcgag
     7861 actcaatcgc ctcttcagac atgttcaaac gttcaaagat tcccttcaag cgttcagaac
     7921 caaaacgttt catcaaatca tcttcaagag ataggtagaa ttgtgactca cctggatctc
     7981 cttgacgacc tgaacgtcca cgaagctggt tatcgatacg acgactttca tgacgttctg
     8041 taccaataac acaaagtcct ccaagttcac gaacaccttc accaagcttg atgtcggtac
     8101 cacgacccgc catgttggtt gcgatggtaa cggcaccacg ttgaccagca ttcatgatga
     8161 tttgggcttc tctatagtgg tttttggcat tcaagacttc gtgaggaaca ccagctgcaa
     8221 ccaatttctt agaaatgtag tcactagttt caaccgctac tgtaccaacc aagacaggtt
     8281 gacccttttg gtaacgagcc ttaacgtctt cgacaaccgc tttaaactta gattcgatac
     8341 ttgcataaag aaggtctgag tggtcaatac gttgaacagg acggtttgtt gggattggaa
     8401 taacacgaat gttgtagatt tcacggaatt cttcttcctc agtcttacct gtacccgtca
     8461 taccagacaa tttcttgtac atacggaaaa ggttttggta cgtgattgag gcagatgtct
     8521 tggtttcatc ctggattggc acaccttctt tggcttcaat agcttggtgc aatccatcag
     8581 aataacgacg accttccatg gtacgacctg taaattggtc gacaatcaag atttcttgct
     8641 cttcgctcac cacatagtca atatcgagaa gcatgatgta gttggcacga agggcgttat
     8701 cgataaagtg agtcaaagcc acgttttcga tgtcatagag gttttcaagt ttgaagtagc
     8761 tttcagccct gtcaatccct gaatcagaca aaccaatagt cttagactgc acatcgatga
     8821 tgtagtcatc tttgttcaaa gattttacat agtggtctgc catgtgatac aactgactgg
     8881 tttcaaccgc attggcacct gatacaatca aaggtgtacg agcctcgtca atcaagatag
     8941 agtcaacctc atcgaccaag gcatagttaa gcggacgttg taccatgttt tcggcgcgaa
     9001 cgaccatgtt gtcacgaagg tagtcaaatc cgatttctga gttagttgag taagtaatat
     9061 cacactcata ggcttctttt ttctccattg gagatttggt agccaagtta atccctactg
     9121 acaaaccaag ccaagagtac aattcaccca tctcagtcgc gtcacgttct gacaggtatt
     9181 cattaaccgt aactacgtga acccctttac ctgaaagggc attgaggtat accggcatgg
     9241 tcgcagtcaa ggttttccct tcccctgtac gcatctctgg cacgtcacca tggtgaagaa
     9301 caatcccccc catgacctga accttataag ggaagagacc taggacacgt ttggcacctt
     9361 cacggacaac cgcaaatgct tcgtaaagca atgaatccag tgattctcca ttttgataac
     9421 gttccttaaa ttcaactgtt tttgctttta gttggtcgtc agtcaaagta gccatttggt
     9481 cttcgtattt gaaaaccttg tcagccatct tttccagacg acggatttct cctttatcat
     9541 tttcgataat tgtttttaaa atattagcca tgtttttcct tactttaaat tccgaatatt
     9601 ttagaatgtt cctttaattt tagcacaatt actgattatt ttcaaggaag aatccccatt
     9661 ttgaaaagga aaaagaggtt agttttcaag ctaacctctc cgacttttat gactgtttaa
     9721 ttgccaaaaa tcggcaaaat ggcaaatgtg ataaataagt taatccaaag agaaagacag
     9781 caaatcagtc caaaaacaaa gagactgact ttcttggaca gcaaggccat gagaatggac
     9841 agaattccaa aagctagcaa gattttctgc atgatgaaga tgtcaatcct tacccccaga
     9901 atcgctctat cccaagggat aaagaagaaa gacagccaga tcagcagaac taaaccaata
     9961 taaaccttgg aatagcgtgt aataaatgat tttagatgtg ccataagcca tctcctagga
    10021 ttgtatatcc tttacatatt gtatatgtcc attttcgaca actaatacat ctgtacaaag
    10081 gtctgaaata tcttccgata tgtgcgacgt caggataacc agaccatttt cttttttcag
    10141 gtaagataaa atgacctgtt tggttaacct cacactcttc tcatccaaag cattcatagg
    10201 ttcatcgaga aagagtatag aaggagagga aataaaggct tgaatcaagg ccattttttg
    10261 ctttgttccc aaggataaat gacggtattc aacgtcttca aattcctgta aatcatagta
    10321 ttgaatccaa taggcaattc ttttttccgt aactttagat gaaaaatacc ttaacagttc
    10381 caaattttct ctcagggata aatgagataa aaactcgact ttttcaatta aaattcctgc
    10441 atcctgaata taatgatttt ttaccccgta aactttacca tcttgaagaa cttttccttt
    10501 gtcaagctta atataaccag caagtatctt taaaagaacc gtcttgccag atccattatc
    10561 acctttaagt ccataaattt ttcctgattg aaacaccaaa ttcaactgat ttaaaatagg
    10621 tcttttccca taaattttct gtccatttat aatttcaagt ctcatcgttt atcctttctt
    10681 aaatttctct tccaagcgac taacaatcaa cataactaat aagcttaaga gtactaaata
    10741 cactgttaca atatgatcca tcatccattc ttgtcccaca aatgccaaaa aatagagaaa
    10801 aaaagaaaaa ccagcgcccg cactaatcag tgctatcaga taagacagta gtaagtacaa
    10861 catcattaac tgagcaagta aaaagaaaag tgcgactgtt ttgaaatgaa ttcctagcgg
    10921 aagtaaacca agaaaccaga caaacaaatc ttgagtaaaa aatttagaaa tgacaaccaa
    10981 tcgtttcctt gtataatcga aaaaggagct tgattgatag cgaatcaagt gatggtaatg
    11041 gtctgccaaa tcactttcaa gtaaaatgac agagagccat cctaccatga aaagcgaaag
    11101 gtttaacaag agtttgggga tttctaaatt cgcttcagaa tagtcattat aggaaaaaat
    11161 tgaaatcaat tctctcttga aaaagataac agcatcctgc ttttgaaccg ctgttgacaa
    11221 tagatagttt atcaaaatca tcatcagaac tatacgaagt agtaaaacga tctttctctt
    11281 tagccaaata ttgttaaaat tcttaaacat tatcttcata ttttttaagt accatataag
    11341 ggacaacata aaggatataa ggagtaaaga gtttcatcaa acttacatcc ccatagctag
    11401 cttgcatcaa actcgtcata ggaaccatat agtaaggcaa ggcactatag agcagcatag
    11461 aaccaagcca aagaaacatc aaataggtga ttgccgaacg agtcacattc cccacataat
    11521 caactatttg taaaaaatag attgcattga tgaaaatacc gattagtagg acacaagtaa
    11581 agaacaaata gctttttatc tctccatcta ggagtctttg taaaccactc ccatcagaaa
    11641 atagagaatt ccattcaaga ggagaaaaag tcccaaagaa ataggttata attgcaatta
    11701 tcagcacagt cactaaatat atcaaacaag ggatacttgc aacttgcaaa gtcaactttc
    11761 tttttaaccc ttgatagctc acttctcttc caatactcaa tcgtaaaact ttatttttca
    11821 gctcaatata ttgaaaacct agcaaaacaa tgatgattgg aataataaat gattgataaa
    11881 aacctagatt gaactggaga atacgaaaat ctaatgctga ttttaataag tcatttttaa
    11941 ttgcttgtcc atcgaattta gctagatcgg gcgcatgaat atcaaaaaag aaataagtca
    12001 tcaaaacatc acgataaacc aaagcaagaa aaagcaaagt aagcaagaca taaacaaatt
    12061 tatgccttgc caaagttaag agaggaatct tcataagcca cctcctacaa atagaaagta
    12121 atataatact caatgaaaat caaagagcaa actaggaaac tagccgcaag ttgctcaaag
    12181 cactgctttg aggttgtaga taagactgac gaagtcagtt acatatataa tccaaggcga
    12241 cgttgacgtg gtttgaagag attttcgaag agtataaatc aatgccttcc gcctttagac
    12301 ttccctagtt cccgtctcaa gtcaagcatt tttttgaaac agatatagtc taaccagtca
    12361 ctagcaaaag cgataagtag taataatagc caaatacccc ataacttgat atctgtcaaa
    12421 tttctcaaga cgatattgaa aaacagaact gaaaaaactg tccaagcaag gctaaaaaga
    12481 gcatagaggg tgatgtaaat ccagtaaaaa tagtaaaaaa ttgggaaaat cttactatct
    12541 ctgtttgcct tttcaatcca gctgaaaaag taaaaacagg gtaataagat tatcatttta
    12601 aacaagtatt ccatcacaaa cctcctcttt taaattgttt tctttagttt cgttctatta
    12661 aatgttatta ccaatataaa ccttggaata gcgtgtaata aatgatttta gaggtaccat
    12721 aagccacctc ctacaaatag aaaccgatat aaatcaatgc cttccaccct tagacttccc
    12781 tagttcctgt ctcaagcgaa acatttcttt gaaacaggaa taagttaacc aattcatacc
    12841 aatagctagc agaataaaaa gaaaccaaat gccccataac ttgatatctg tcacatttct
    12901 caagacggta ttgaaaaaca gaactgaaac aactgtccaa gcaaggctaa aaagagaata
    12961 gaaggggatg taaaaccagt aaaaataata aaaaattgga aaaaacttac tatttctgtt
    13021 ggccttctca atccagttat caaaataaaa gtacggtgct aaaagtaaga atttaaacaa
    13081 atgttccatc accgacatca ccctccttct tttgatagcg ttttctatta ttttattata
    13141 tcaaaaaaat ccggaactgt cattccagat tctacttttt tatttgcgtt ttcttgcgat
    13201 gagatgaatc ggtgttccct caaaaacaaa ggccttgcgg atttgatttt ccaagaaacg
    13261 caggtaagaa aagtgcatga gttcttcttc attgacaaag atgacaaagg ttggtggttt
    13321 ggttgccact tgggtcgcat agaaaatctt gagacgtttt cctttgtctg tcggtgttgg
    13381 gttgatggca atggcatcca tgatgacatc gttcaagaca gctgatggaa tacgtgtatt
    13441 ttgactttcg ctgatttgct taatcatctc aggaagtttg tggagacgtt gcttggttaa
    13501 agctgataca aagataatcg gtgcgtaagg caggtattgg aactgctcac ggatatcttc
    13561 ttcccagttt ttcatagtgt ggttatcttt ttcaagcgta tcccacttgt tgaccacgat
    13621 aatcatccct ttaccagctt catgggcaaa tcctgcgata cgcttgtcgt actcacgaat
    13681 gccttcttcc gcattgatga ccatcaagac cacatctgaa cggtcaatag cacgcatggc
    13741 acgcataaca gagtatttct cagtattttc ataaacctta ccagacttac gcataccagc
    13801 cgtatcaatc atggtaaact cttgaccatc tgtatctgta aagtgggtat caatggcatc
    13861 acgagttgtt ccagcaacag gactagcaat aacacggtct tctcccaaga tagcattgat
    13921 caagcttgat tttccaacgt taggacgacc aatcaagcta aacttaatga catctggatt
    13981 ttcttcctca tattcatttg gaagattttc tacgatcgca tctagcacat cccctgtacc
    14041 gattccatgg acagatgaga taggcaatgg ttcacccaaa ccgagagcat agaaatcata
    14101 gatatcattt ctcatctcag ggttgtccac cttgttgact gcgaggataa ctggtttgtg
    14161 ggtcttataa agcttacggg ctacgtattc gtctgcatcg gtaattcctt ccttaccgga
    14221 cacgacaaag acgataacat ctgcttcttc catggcaatt tctgcctggt gcttgatttg
    14281 ttccatgaaa ggagcatcga catcatcaat tcctcctgta tcaatcatgc taaaagaacg
    14341 attgagccac tcacccgttg cataaatacg gtcacgtgtc actccttcga catcttctac
    14401 aatggagatt cgctcaccag cgatccgatt aaatagggtt gatttcccaa cattgggacg
    14461 tcctacaatg gcaatagttg gtagggccat aatttctcac tttctacaat aatttcttct
    14521 gttcaagatt ttttctagtt gagcttggtt cagcttgacc aaactgttct gctaggcgct
    14581 gactccagct tgtggtcgca cgcgccccag catagtcagc ctgaacacgg tcataagctt
    14641 ggattgcctc agttgactgt tcttggtatt cttcctcaaa gacaacattc tctagtggca
    14701 gtctcggttt catatcatga tgttgatttg gcacacccag tgccatccca aagacagaat
    14761 aggtgtagtc aggtaggtta aagagctctg ccacttcttc agacttgtat cgaaccaaac
    14821 cgataatcac accaccatag cccaagcttt cagctgccaa caaggcgttt tgtccagcaa
    14881 gagctgcatc gaccgaacta atcaagagac cttccacacc ttggggttgg aaggtgtcgg
    14941 tatgaagtcg ggctcccttt tctgctcggt tcaaatctcc gacaaagaga aggaaaacag
    15001 cagactggcg aatggcttct tgaggtacca attcatacaa ggcatctttc ttctcttgac
    15061 ttcgtaccac aatcacagag taggattgga aattcttcca agatgatgcc atctgggctg
    15121 ctgtcaaaat ctcatttaag tctacttggg gaatttcttg ctctttaaac ctgcgcactg
    15181 aagtatgagc cttcatcaat ttaatggttt ctgtcatcga cggtttactc cttctaaacg
    15241 agtctcctca gccaaataac ggatgcgttc catgacccgt ctggcttccc aggtttcgtc
    15301 atttccatgt ttcactttcg caaaatgctt ctccaaatct tcaaagttga agttggatgt
    15361 gaaaaaggtc ggtaaatttt cctgcatccg atattggaga atgacctgca ggatttcgtc
    15421 acgcacccaa gcggttgatt gctcggcgcc aatatcatct aaaatcagga cctcagacag
    15481 cttaatctca tccaccaagg tcttaacatt gccatcactg atagcatttt tgacatcaat
    15541 gacaaagcta ggatagtgga ggagagttga tgaaacacca cgtttttctg ataaatcatg
    15601 agctaaggcc gccaccatga aacttttacc cacaccaaag tctccatata agtaaagacc
    15661 ttttcgaata gctggatatt gctccacaaa ggctaatagt ttttcaaaaa ctggcaagcg
    15721 tcccaaatca tccaaatcaa cttgagccaa actagctttt ttgagactgg ctggtagatt
    15781 gattagcttg agacgattct taatagccgc ttctttttca gccgcgatta gctcaggagt
    15841 ttcttcatat gaaacatctg catagccatg attcttaacc aaaatcggct tgtagccttt
    15901 ggcaatataa tccgtatccc cacggagaaa cttgtcacgc tcggtgatgt actgattaaa
    15961 cttggagata ctgcgattta attcctctgg agtcaaggat tcttgctgga taaaagccgc
    16021 aacatcaggg tccttcatga ttttctggac caaatcttga taataaaaac ggctgggttg
    16081 acgtttgagt acgtctccga cactttccat ctaatctcct cctttttcta atcgagctaa
    16141 tagttcttgt ttcttacgtt ctagttccag acgagtttcc tcgctggttt cattcttata
    16201 atctgggtta ctccatttag gaatattggt ttttgctggg gcagtctgat tctgtttttg
    16261 tgtttttgtt ttctgccctc gctcacgaat tcgtaaaacg gcctcttctg ctgaatgtat
    16321 cttttgatag gcatagtcat tagccacctt catggcatat ttctcattga tgtttgccga
    16381 atccacctta ttaaaggtca acaagagaat gatattgatg acttcgtcca gcaagcccaa
    16441 gccagccatc tgttgcaaaa gttctctttc tgtttgggta atggtcccct tgcgtgtttg
    16501 cttgatttct gctaagaact gcagggcagt cttactttta gcttccttga taatggttgc
    16561 ttccttagga ttaaagtcag aggaaactgg tttttgagca attttttcac gcatgcgttt
    16621 ggttgaaata acctgagaaa cagctgttga cttggccaat tgataggttt caaaccaagt
    16681 ccatttcttc tcctcggcaa tagcaaagag gtttaagaca tcggactgct catccgcaaa
    16741 acgaagtcca tctcgagcca tcagctggcg aaaatgttcc aagtcaaaat cattggccac
    16801 tttcttcttg agaccaaggt cttcttgact gcctagttct gccaattctg gaaagacttg
    16861 attgagtgag acaggtattt cttcaccatc agcactttca actttcaaat cctccacagc
    16921 agagtcgcca atctttttct ctaagagtct gcgataaaca ggatgctcca agaagtcttg
    16981 actagataga ggagcatgga gggctagctg ataaacatcc cccttttgat agagggtcaa
    17041 gagattaaaa gcagataaga ttttcaatga ttttatcagt ctatccatcc caaagttgag
    17101 atgattgagg atacttgaaa aaagatattc ctttctacca ttatcccaaa aactgattgt
    17161 ataaagataa aggctcagtg cctcctgacc gataatcggg aggtagcact gtaccagaga
    17221 tgaggtatct tgcgacaccc gattattctt tagataagaa aaacggtcaa ttggcttcat
    17281 ttatctttcc tttttctttt tagaggactg ggtgatttgt tggagcaagc tctctaactc
    17341 actgacatcc ttaaaactac gatagacact agcaaaacgt acataggtaa tctcgtccaa
    17401 ttcagccaac tcctccatga cgagtgaacc aatgtcctca ctttgaattt cattttcatt
    17461 tcgaccacgg agtttctgtt cgatacgatt gactaccatg ttgatttcat cacttgacac
    17521 aggacgtttc tgggctgagc ggataatccc attaaagatt ttatctctgg agaattgttc
    17581 ccgtgtacca tcttttttaa caaccactaa ggttctttct tctactcgtt cgtaggttgt
    17641 aaaacggtgt tggcattcgt cgcactcacg tcttctacga atggtgttcc cttcttctgc
    17701 ttggcgacta tcgataacac ttgacttggt agccccacat tttggacaac gcatcctttc
    17761 cctccttatc gttttctttt cattatacca ttttttaagc gattcccaaa acaattcttc
    17821 tttttgcttg acaagttttt tgttttgttg tattatttag ttaagacaaa aggataagag
    17881 aaaggagact aagatgtcct ggacatttga caacaaaaaa cccatctatt tacagattat
    17941 ggagaaaatc aagcttcaga ttgtttccca tacactggaa cccaatcaac aacttccaac
    18001 cgtgagggag ctagctagcg aggctggtgt caatcccaat accatccaaa gagccttatc
    18061 agaccttgaa cgagaaggat ttgtctacag caagcgaaca actggacgat ttgtgactaa
    18121 ggataaggag ctaatcgctc agtcacgcaa acaattatca gaagaagaat tggaacactt
    18181 cgtttcctcc atgatccatt ttggctatga aaaagaagaa ctaccaggcg tagtcagtga
    18241 ttatattaaa ggagtttaag cctatgtcat tactagtatt tgaaaatgta tccaaatcat
    18301 atggagcaac accagccctt gaaaatgttt ctcttgacat tccagctgga aaaattgtcg
    18361 gccttcttgg gccaaacggc tcaggaaaaa caaccctgat taaactaatt aatggcctct
    18421 tacaaccaga tcaaggacgt gtcctcatca acgacatgga cccaagccca gcaaccaagg
    18481 ccgttgtagc ttatttgcct gatacgacct atctcaatga gcaaatgaag gtcaaagaag
    18541 ccctaaccta cttcaagacc ttctataaag atttcaatct tgaacgcgct catcatctac
    18601 ttgcagacct gggcattgat gaaaatagtc gtctcaagaa actatcaaaa ggaaacaaag
    18661 aaaaggttca actgattttg gttatgagcc gtgatgctcg tctctatgtt ttggatgaac
    18721 ccattggtgg ggtggatcca gcagcccgtg cttatatcct caataccatt atcaacaact
    18781 actcaccaac ttctaccgtt ttgatttcta cccacttgat ttctgatatc gagccaatct
    18841 tggatgaaat tgtcttccta aaagacggaa aagtcgtccg tcaaggaaat gtagatgata
    18901 ttcgctacga gtcaggtgaa tccattgacc aactcttccg tcaggaattt aaggcctaag
    18961 caaaggagat tatttatgtt ttggaattta gttcactacg aatttaaaaa tgttaacaag
    19021 tggtatttag ccctctacgc agccgtgcta gtcctttctg ccctcatcgg aatacagaca
    19081 caaggcttta aaaatctacc ttaccaagaa agtcaggcta ctatgctact ttttctagct
    19141 acagtctttg gtggcttgat gcttacactt gcgatttcaa ccattttctt gattattaaa
    19201 cgcttcaaag gtagtgtcta cgaccgacaa ggctatctga ctttgacctt gccagtttct
    19261 gaacaccata tcatcacagc caaactaatc ggtgccttta tctggtcatt gattagcacc
    19321 gctgtattgg ctctaagtgc tgttattatt ctggctttaa cagctccaga atggattcct
    19381 ctttcttatg tgattacatt tgtagaaaca catctccctc agatctttct tacaggtata
    19441 tccttcctac taaataccat ttcaggaatc ctctgcatct acctggctat ttccattgga
    19501 cagcttttca atgaataccg tacagcactc gctgttgcag cctacattgg tatccaaatc
    19561 gtcattggat ttattgaact tttcttcaat cttagttcta atttctatgt caattcactg
    19621 gtaggactca atgaccattt ctatacggga gcaggtatag ccattgttaa agaactcata
    19681 ttcatagcta tcttttatct cggaacctac tacatcttga gaaataaggt taatttgctt
    19741 taaataattt ttacctagat atgtaacata ctcatagaac aaaagagacc aggcaaaaag
    19801 tctttaaaat tagaaaacgc atagtatcaa gtgttaaaaa tccttgatat tatgcgtttt
    19861 ttgtgagctg acttatttcc tttcactata tcgcaaaatg aaataagaac ggaacgatgg
    19921 gattttggaa ttcaaatcaa tttataagaa tgttttagaa gtaatattat cctattccag
    19981 attcagttca ctatacaatt gagttttcaa gcaacctgtt tacataatgt gtacatcatt
    20041 aggttcgtga ttccaccctt ttcaccttta aaaacctcgc tttcgcaagg ctcttctatt
    20101 tataagataa ggcacgttta aaggttttcc aaatccctaa atcatccgtt tgaagaacga
    20161 gactagcata catgcgtccg ataaatcctg ttgctaccac cgcaaaaatc actgtaatag
    20221 caagtgaaat ccatgcttct gctccccccg catagccatt aatcgttcga aacggcataa
    20281 agaaggtcga aataaaggga atataagaac caatcttcaa gaggagattg tcaccagctg
    20341 cacctagagc tgtcactcca aaaaaaccac ccataatcaa aatcatcaaa ggcgacaagg
    20401 ctttccctga gtcctcagga cgagaaacca tagatcctag gaatgctgcc aagactacgt
    20461 acatgaaaag actgatcaaa ataaagagca aggtattcag tgagatagca tctcccaagt
    20521 gatccaaaat accagactga gccaagaatg gcaaatcttt aaagagcaaa acggcagcca
    20581 gaccacctac aacatagatc ccaatatgcg ttaaaatcac tagaaacaga gccatcatcc
    20641 gcgcatagaa atagtgactt gcccttatgc tagaaaaaac gacttccata attttggtgc
    20701 ctttttcact ggcaacttcc tgagctgtta cacccgcata ggtaatcaga atcatataaa
    20761 gaaagaatcc taaggcacct gctgcaattg tttgaataaa ctttttattt tccttggctt
    20821 catcaatctt ttctgtgaat tgaattgtct gctctaagcg tttttcctgc tcttgagaca
    20881 aggaagcagt tgaacgatta agctgatttt gcagttcatt gagtgtacct gtaacctcaa
    20941 atttaattcc attttcaagc gatgtttcgc catgataaac tgcctttaga acactatctt
    21001 cttgatcaat ggtcaaataa ccttttaatt tttcttcttt aattgcttct ttggcacttg
    21061 cttcgtcttt atagtcgaag ttaacaccat ttacattctt cagtccttct gctacagatg
    21121 gcactgttgt cactactgcc actttattat ttttagccat agaagaacct tggagatgcc
    21181 caattcctac agagattcct aaaaagagga acggcgaaat caccataaag aagaaactcc
    21241 atgactcgac atgtcgaaga taggtttcct tgattacaac ccacatattt ctcatacttc
    21301 cactcctgat tctagtttaa agatttcatc gatagttggc gcttgttggt caaatgttgc
    21361 gatatattga ccttgagtca agattgggaa gagttccctt ccagcgctct catcctccaa
    21421 aatcaatttc caactgcctt gtttggtcaa gctcacctgt ttgacatgag gaagattttc
    21481 caattcttcc ttgcttcgtt cacttgaaac aaagagacgc gttttcccgt attgattgcg
    21541 gacatcctga actggtccgt gcaagaccac acggccatct cggatcatca gaatatcgtc
    21601 acaaagttcc tcaacattgg tcatgacatg gtcagaaaag ataatggttg ctccgcgctc
    21661 tttttcctga aaaatgactt gtttgagcaa ttctgtatta actgggtcca atccactaaa
    21721 aggctcatcc aagataatca ggtctggttc atgaatcaga gtaataatga gctgaatctt
    21781 ctgctgattt ccttttgaca gactcttgat tttatctgtc agctttcctt tcacttccaa
    21841 cctcttcatc cattgaggga gtttttcttt gacttctttg gcatccatgc cttttagagt
    21901 cgccaagtag cgaacttgtt caagaactgt caatttaggc atgagactgc gttcttcagg
    21961 cagataacca atccgagcat aggtctcctg acgaatatcc tgaccatcca gaccgatttc
    22021 tccctgatat tctaggaatt tcaaaatact atggaaaatc gttgtttttc cagcaccatt
    22081 ttttccgact agtcccaaaa tacgacctgg tcgcgcttga aagtcaatac caaacaaaac
    22141 ttgcttggag ccaaaacttt tctctagact tcttacttct agcatctttc acctccgaaa
    22201 tttcttgcac tcattatact cctttttgat agcctttaca atgtttttgt ccatttttag
    22261 aagactattg ctgtgtaaaa tatggcctgg agcactttta tactcaatga aaagcaaaga
    22321 gcaaactagg aagctagccg taggctgctc aaagtacagc tttgaggttg cagat
//
LOCUS       DAVBCG010000018        22195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBCG010000018 DAVBCG010000000
VERSION     DAVBCG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.018"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(70..2028)
                     /gene="ftsH"
                     /locus_tag="VQ495_000603"
     CDS             complement(70..2028)
                     /gene="ftsH"
                     /locus_tag="VQ495_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2530597.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2044..2586)
                     /gene="hpt"
                     /locus_tag="VQ495_000604"
     CDS             complement(2044..2586)
                     /gene="hpt"
                     /locus_tag="VQ495_000604"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2530598.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2590..3867)
                     /gene="tilS"
                     /locus_tag="VQ495_000605"
     CDS             complement(2590..3867)
                     /gene="tilS"
                     /locus_tag="VQ495_000605"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2530599.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETIFMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIKNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3864..5132)
                     /locus_tag="VQ495_000606"
     CDS             complement(3864..5132)
                     /locus_tag="VQ495_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2530600.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5252..5620)
                     /locus_tag="VQ495_000607"
     CDS             complement(5252..5620)
                     /locus_tag="VQ495_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2530601.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5613..5879)
                     /locus_tag="VQ495_000608"
     CDS             complement(5613..5879)
                     /locus_tag="VQ495_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2530602.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5937..9446)
                     /gene="mfd"
                     /locus_tag="VQ495_000609"
     CDS             complement(5937..9446)
                     /gene="mfd"
                     /locus_tag="VQ495_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2530603.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEKNSI"
     gene            complement(9447..10016)
                     /locus_tag="VQ495_000610"
     CDS             complement(9447..10016)
                     /locus_tag="VQ495_000610"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2530604.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10087..11202)
                     /gene="ychF"
                     /locus_tag="VQ495_000611"
     CDS             complement(10087..11202)
                     /gene="ychF"
                     /locus_tag="VQ495_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2530605.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIIEFRFNV"
     gene            complement(11286..11480)
                     /locus_tag="VQ495_000612"
     CDS             complement(11286..11480)
                     /locus_tag="VQ495_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2530606.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11545..12681)
                     /gene="dnaN"
                     /locus_tag="VQ495_000613"
     CDS             complement(11545..12681)
                     /gene="dnaN"
                     /locus_tag="VQ495_000613"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2530607.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12840..14201)
                     /gene="dnaA"
                     /locus_tag="VQ495_000614"
     CDS             complement(12840..14201)
                     /gene="dnaA"
                     /locus_tag="VQ495_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2530608.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVSMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14414..15172)
                     /locus_tag="VQ495_000615"
     CDS             complement(14414..15172)
                     /locus_tag="VQ495_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2530609.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15230..16411)
                     /locus_tag="VQ495_000616"
     CDS             complement(15230..16411)
                     /locus_tag="VQ495_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET2530610.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16594..17073
                     /gene="rlmH"
                     /locus_tag="VQ495_000617"
     CDS             16594..17073
                     /gene="rlmH"
                     /locus_tag="VQ495_000617"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2530611.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17117..17190
                     /locus_tag="VQ495_000618"
     tRNA            17117..17190
                     /locus_tag="VQ495_000618"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17151..17153,aa:Arg,seq:tct)
     gene            17355..17480
                     /gene="comC"
                     /locus_tag="VQ495_000619"
     CDS             17355..17480
                     /gene="comC"
                     /locus_tag="VQ495_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2530612.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            17501..18815
                     /locus_tag="VQ495_000620"
                     /pseudo
     CDS             17501..18815
                     /locus_tag="VQ495_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            18812..19564
                     /locus_tag="VQ495_000621"
     CDS             18812..19564
                     /locus_tag="VQ495_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2530613.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19616..19687
                     /locus_tag="VQ495_000622"
     tRNA            19616..19687
                     /locus_tag="VQ495_000622"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19649..19651,aa:Glu,seq:ttc)
     gene            19693..19766
                     /locus_tag="VQ495_000623"
     tRNA            19693..19766
                     /locus_tag="VQ495_000623"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19727..19729,aa:Asn,seq:gtt)
     gene            complement(19807..20349)
                     /locus_tag="VQ495_000624"
     CDS             complement(19807..20349)
                     /locus_tag="VQ495_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2530614.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20528..21295
                     /locus_tag="VQ495_000625"
     CDS             20528..21295
                     /locus_tag="VQ495_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2530615.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21380..22060
                     /locus_tag="VQ495_000626"
     CDS             21380..22060
                     /locus_tag="VQ495_000626"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2530616.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         6868 a         4086 c         3621 g         7620 t
ORIGIN      
        1 cattttctcc attctgttcc ctttagctcc taaactgcac aaaaagagag gctccagcct
       61 ctctcagggt tatttttcgt cattcatttt tgactttact tcatcatagg atagtgcatg
      121 agattcctct tctactgctt caggcatctt tcctgtttcg taaagagctt taatttgtgt
      181 actatccaat gtttcgtatt tcaataatgc ttctgcaatt aacttgtgag tttcacgatt
      241 tgactgaata atttcagcag ctttatttcg tgcctcattt aataatgaac gaacctcttc
      301 atcaatttca taagctgttt gttctgaaat tgatttttga ggactctgtg caccaagcat
      361 agcatggttt ccttcatatt gtactgggcc aagtttttca ctcataccgt actctgtaac
      421 cattgcacgt gccatttgtg tcgcttgttc aaagtcgttt gaagctcctg tagtttggac
      481 attaaagata atttcttcag ctacacgtcc acccattaag ccagccaatt gctctttcat
      541 atcttcttta gatagaagca tttgatcctc tttaggaagt gcaatcatgt atccgcctgc
      601 acggccgcgt ggtacaattg taaccttatg gacaacgcga gcattcgaca agactagacc
      661 aacaatggta tgtcctgcct catggtaagc aaccaattct cgttcttttt gtgaaactgt
      721 cttatctttc ttagaaggtc cagcaataac tctatcttct gcttcatcaa tatctgaagc
      781 atcaattatc gatttattgc gacgagcagc aactaaagct gcttcattca agacattctc
      841 taaatcagca ccaacaaagc ctggagtttg ttgagccact aatttcaaat caacatcttc
      901 tgctaaaggc ttgttcttag cgtgaacttt caagattgct tcacgacctt taacatcagg
      961 acggccaacc aatacttttc tatcaaaacg tcctggacgc aaaagggcag gatcaagtac
     1021 atctgaacgg tttgtcgcag cgatgacgat aatcccttca tttccctcaa aaccatccat
     1081 ctcaatcaaa agttggttca aggtttgttc acgttcgtca ttacctccgc cgagaccgac
     1141 tccacgttga cgtccaacag catcaatttc atcgataaag atgatagctg gtgctgcttt
     1201 tttggcatcc tcaaaaagag agcgaacacg actagctcca actccgacaa acatttctac
     1261 aaagtcagaa cctgagatac taaagaatgg aacacctgct tctccagcga ctgccttagc
     1321 aagcaaagtt ttacctgtcc ccggaggtcc ctccaaaaga acacctgctg gaatacgggc
     1381 tccaagtttt gtgaatcgtt ttggatcttt taagaactca acaacttcaa ctagttcttg
     1441 tttttcttcc tcagctccag caacatctga aaatcttact ttaatatctt ctttatttgc
     1501 tgctttagcc ttactacgtc caaaactcat tggattacgg ccattgcctc ctcccatatt
     1561 tcccatcata gagaatagga agaagaatag aattccaaat ggcacaatgg atacgagtag
     1621 attaatccat ataccacttg aactttcatg cttaacagtt acttctgctt tatggtcagt
     1681 agcaagtttt tgcaattctg atacggtagt atctgcagga agaatagtgc tggtaaattt
     1741 ctctacctta gtaacagatg gcgtgaaaaa ctgaatacct gtttcttctt tacttgtttt
     1801 aggattttta tagacaccag aaacttcgat aacactacca tttggttggt aagttaattc
     1861 ttttacatta ccatcggtaa tttcttgtac caactcagta tagttgattt gctgacttcc
     1921 tcctgagtta ttcccagaat agaaatactg gaatcctgtc acaaggaaaa agataaataa
     1981 taaccataga aaaggatttt taattaaacc attattttgt tttttcatta aagattattc
     2041 tttctaattt gaatacactt cctctttcaa tactccaata taaggaagat tacgataatt
     2101 ttctttgtag tctaaaccat aacctactac aaactcattt gggatagtaa agcaggtata
     2161 gtctgcctca atttctacaa cacgtccttc tggtttatcc aacaaggttg caattttaac
     2221 agaagctgct tctctttctt taaacatatc tcgcaaattc ttcaaagttt gacctgtatc
     2281 aatgatatct tctacaaata gaacatgtct tcctttgata tcttgagtca catcttgttt
     2341 aatattgata acaccactac ttgctgttcc accatggtag ctagaaacca tcatgaagtc
     2401 catttcaata tgtgtatcaa tatgtttgac caattcagcc ataaaaggaa tagatccttt
     2461 taaaatccca actaagattg gattttttcc tgcataatct ttagttaatt gagcacctag
     2521 ttttttagct gcttctgtaa tttcatcgtg tgaaacgagg acttttttaa tatcgttttc
     2581 taacattttt tacctatcta ttttttctat ataaagtaca gtgttcatta tatcattttt
     2641 cgttttttta ctcaaattat tggtcgcaat tcccaaaatt gagacaattt caccaaattg
     2701 ctcaataata agagcagagt ttctcttttc catagggatt ttcaaatcaa taaataaacg
     2761 tctgagtttt tttctatgcc catttttaat caaaacatct cctgtttttc gatgacgaat
     2821 gtgtatggat gtttcacgtg aaacaggtat ttgttgaatt aattcacctt ctaatggaag
     2881 tccaaaagaa aataaatatc cttgataagc tacctgattt tgatagtgta acacaagttc
     2941 atcttccttt tcatcagcct gcggactgat tttacaaatc tgaaactgtt ggtactcttt
     3001 tatcaattca tagccatttt taatcggatg acgatactgg cttttagatt ttaaaatctg
     3061 ctgaacttca gcaaactgag cttttgtaag attcaaatct ggaaaacgat tcagataagt
     3121 ttgaagtaaa actctttgtg tagactcaga gtaagaaaat aactgctgta aatcttccac
     3181 attaatattg ttagataatt cagctattgc caaatcataa tctaaaattt cattgccaat
     3241 gcctaagatt gcatccctaa atcgaggatt ttctttttcc aattctggta agtaagaatt
     3301 tcgaatacga tttcgaaaat aatgattctc ctgatttgat gtatcttcaa agtgaaaaat
     3361 tgatggaaag tcttttttct gaaaatgcaa gaagggacga atgatttcta tctctccgac
     3421 tacttgcttc tccttaattc ctgatagata gcgcaagcga gttcctcgaa tcaagcgcat
     3481 aaaaatcgtt tccacctgat catcagcatg gtgggcagtg actaaagctg tcgcacctgt
     3541 ctttttcatg acctcttgaa aaaaatcata acgaaaattt cgtgcacgcg cttctgaaaa
     3601 ttctcctgaa aaattgctga tataaatagg aagctctgct tcagcagcca acttccttaa
     3661 ttccttttct tcccaatctg attcaattct ctgcttatga ttcacatgag ctagaatcaa
     3721 ttcaatctct aactcttttt gataagtaga caatacctta aatagaaaca tggaatctaa
     3781 tccaccagaa agagctagaa ccgccttagc atgctttttg aaatatccct tcttgagaaa
     3841 atgatttaaa aaatctggtt ccctcatttt agaacctcat aaacatcctt ggctatctta
     3901 gaaatcgtat cataatcaga attcttagtg aaaatagaaa gaataaatgg agaatctgca
     3961 tagacaacac ccgtatcatg cttaaattca tccgcatctc caattttatg agctacttta
     4021 acagaaacac ctttggcaat tcgctgacta tcaaaatctg ttttagtcaa agactctagc
     4081 acaaatccat tttgattata aatagcttcc ataaacttcc cggccatctt agaagaaatc
     4141 aatttttctt ttggatccca atcatctccc ataatggcag acatcttgga tttgaatgtg
     4201 gcatcagatt ggtttgaaat gtaatatccc aatagattat gagctacatt atcagattct
     4261 tttgatactt tcgtaattaa atcctttaaa gaatattctt tattatcttc ttttttagga
     4321 agactaccac ttccctctgg tttataagaa cctggaaaat cattgactgc agatacgtat
     4381 tttacagtcg tatctaactg ataaagaccc tcatttattt tttcttgcgt ataatagaga
     4441 taagagagtt tcaaaacgct ggctgcatac atcttttcat cttgattgat accagcttct
     4501 tttccagtag tcagttgctt aacataaata gagaaagaat ctttctgata tttttcagat
     4561 aacatttctt gaactttact catccgatta tcttcttcag aagttgattc tttagctacc
     4621 catccagcct gatcaatatg tagaaattct cttccttcta caaacatggt cttgtcgatt
     4681 gatacttgcg aataagctga taaggatgat ttcacttctt ttaaatcata aggactattg
     4741 tacagtttaa agtcagattc taaccatact ttttttattg ttggagttac ctctgattga
     4801 tcatataaaa atcgtttgtc cgcagctata aattgatgat ttgatagctt aaatactgga
     4861 attccttgtt tatttaagcg ccactcggtt atttgaaaac ttgttttggg agtcaatttc
     4921 ccagattcca ctactaaatc ttcattcgca taaacaggaa cctctccata aaccatggga
     4981 gaacttaatt tttctctaaa ataaatacca aagtcagatt gtgaaaggta ataaatttct
     5041 ttcgaagtat agacgacttc tttttctgtg ctaacgactt ttgaaatggt caaaaaactt
     5101 ggtagcaaca aaataattaa gaatttacgc attcttcctt tccttttctt cttgtaaacg
     5161 caataattga tttttatcag ccaattcttc cagcttttca tctgacaaac tcaaaaattg
     5221 ctctattacg tctaataaat tttccatttt atcacctttg aagcaagtca ggaatcgtat
     5281 aaactttttc cctcgactta gaataatagt acttcgctcg tgtatatttg gcagcataat
     5341 cttcatcttt caacttggta gcaaatgctg tctccttatc cttttcatca ctcaaagttt
     5401 gatactgagt ttgcaagtct gctaattgct gacgtctttg gagtaattgc tgataactct
     5461 gcgctaaatt aaaagttggc aagataaata atagcataat caaaatcaat acccctccca
     5521 taaaacgatt ccgtttttgt cgttctttca tcaggtagcg acgacgttgg tattcatttt
     5581 gaataaaaga attattcaat tgtacaatat ttttagacat tttcttctac ccgtgtttca
     5641 ctgataattt catacattcc tgctgcatct tctttttttg tactatcttt catctctagt
     5701 acttttacaa gcagcaactt attgccaaag cgaatttcaa cttggtcatt aactttcaag
     5761 tccgttgaac ttttggccaa gattccatta accttgattc tacctttatc tgctacttcc
     5821 tttgcgactg tacgacgctt gataattcgc gatactttta aatatttatc taatctcatc
     5881 cttattacct caatttatta ttgtaccatt tttatcttct ttatagaaga aaaatatcaa
     5941 atggaatttt tttccttaga ctcttttatc tctaataaac tttctccaaa aatcagcaga
     6001 ccttctaaaa tttcataatc tttcttattt tggacatcaa atacaagctc cattaatccc
     6061 ttattctcag cgatgcctgc ttttaagttc gttacggata aagctttaaa ataatcttga
     6121 gctaaaaaca gtcgttgagt gactttttca aattgaattg taattttatt atcttttctt
     6181 tccacacgtt gaacaaagac cttgtccaag tatgatttga ccaaaccaat ctctaaaaga
     6241 taggctacta catctgggta ttctccaaaa cggtctatca actcctcttg taactcttca
     6301 taattgacac ggttgtcaat ttgacgaatt ttcttgtaaa tttcaatctt atgtcgttga
     6361 tcagaaatat aagtatcagg aagataggca tcaatttgca aaatcaactc agcattccct
     6421 ttggttcttg tgttagcgtt agcattaccg tttcgtttag caatagcttc ctctaataac
     6481 tgcgaataca attcaaaacc aacagaatca atgaaaccag actgggattt tcctaaaaga
     6541 tttcctgctc cacgaatcga aagatctcgc attgcaatct taaagccaga gcccaattct
     6601 gtaaatcctt taatcgcttc taatctcttt tcagagactt cactgattga tttttctgga
     6661 cgatacatga gataagcata agcaatacga ttactacgac cgactcttcc tcttaactga
     6721 tataaggttg acaagcccat atggtccgca ttttcaataa ataaagtatt agcatttgga
     6781 atgtccaccc ctgtctcaat aatagtagtc gtcaccaaga tatcgtattg tccctcaata
     6841 aagtctaata gagtattttc caactggact tcactcattc gaccatgaac atatccaatc
     6901 gaagcctccg gaatcaactc ctgtaattct gaaaccttct gaacaattgt gtcaactttg
     6961 ttgtaaagat aataaacttg acctccacgc tccatttcac gcaagacagc atcacgaatg
     7021 acactatcat tcttttccaa aacataggtc tgaacaggat agcgattagt cggcggagtt
     7081 tcaataacag ataaatctct gattcccagc atagacatat ggagggtacg agggattggc
     7141 gtagcggtca aggttaggac atccacttgt ttcttcagtt ctttcaaagt ttccttatgc
     7201 ttgacaccaa atcgctgttc ctcatcaata atcatcaagc ccaaatcagc aaacacaaca
     7261 tcttttgaca aaacacgatg tgttccaatc aaaatatcga cttgaccgtt tttcaatttt
     7321 tcaagtgttg cagtctgctc ttttttactt ctaaagcgac tcaacacatc aatattaact
     7381 gcaaaatttt ggaatcgttc cttaaaattc gtatagtgct gttgcgctaa aaccgtcgtc
     7441 ggaactagaa tgacaacctg tttgtgatca ttgactgctt taaaggctgc acgcatagca
     7501 acttcagtct ttccaaaacc aacatcccca actaaaagtc gatccattgg ctgagaagcc
     7561 tgcatatccc tcttgatttc ctcaatacta cgaagttgat catccgtttc aacataaggg
     7621 aaagcatcat caaaggcatc ttgatcatca tcatcagctg agaaagcaaa acccttcaac
     7681 tgactacgtt cagagtagag tttgattaaa tcatcagcta tatcctctac ctggttctta
     7741 accttttgct tggccttttt aaaatgaccg tcatttaatt tattgagttt tggagcttta
     7801 ccatcacttg aaatatattt ggacagtaga tgaatctgtt ccacggggat agaaatttga
     7861 tcaccatttt ggtattggac actgacataa tcgcgatgaa ttcccttgat ttcaatggtt
     7921 tcaattccta gatattgacc aatcccatgg atatgatgga caacatagtc ccctttttca
     7981 agttcattgt aatcttttaa tctctctgca tttgaaacat gttgtcttcg aaaacgacgc
     8041 tttaatttct tttgaaaaat ctcatgttca gttatcaata aaatcttttc atctacaaaa
     8101 tgaaaaccat gtctgagatt accctctatt aaatttacag attctttaca gatacttgtc
     8161 ttatctctag aatccaattt aatctgatat tcctctaaca tatcctccaa tgttttactt
     8221 cccattgaat tgctagactg cagaataatg gtgtaatcca tttttttata tcgttcaatt
     8281 tcttctttta gaaaagaaaa ctgattgaaa aattcctgta taggatattg attgaattga
     8341 taaattttgt caaatttgag atttcctaaa cccttttgaa gattagagaa aaaggtcact
     8401 ggactttgtt ttttatagat ttgttcaata tcagaaaaat actgcatatc agaaaatgct
     8461 ttactattct gtaattcttc tgtaaagtac tgcgctaatt ctctttcaaa gacttcatac
     8521 tgattcatca atttttgata atcatcaaag aatattggag tatctttttc aatataatca
     8581 aagacagtcc atgtcttatc atagcacaaa gataaaaact tccgagaatc tgcatgactt
     8641 tgtttttggt gaaaacttga aagaatttct tctaggtatg atttcaaaat aggtgataaa
     8701 gtttttgaaa tttgtttttc taaagctgac tgtcctcgtt gataatcctt ttctctcaaa
     8761 agcatatcac tagctggaaa gatagtgagt tctgtcttat tttctttcga taattgtgtt
     8821 tctacttcaa atgacctgat accatcaatt tcatcaccaa aaaactcaat tcgacaaggt
     8881 tctaactggg atatttcaaa aatatctaaa atatctcctc gaagactaaa ttcgccctga
     8941 gtttgtactt gagtaacttt ttgatagcca ttttccttta actgatggat aaacgcgtgt
     9001 tgagcatatt cttcaccaac tgagattttt acaatactat ctttgaatgc attgggagac
     9061 ggtaaaatca atcgacttgc tgcgatatta caaactaaaa tccctttctt agatgaatca
     9121 gtcaaaaaac gcaaggcttc aacccgtgaa ataatttttt cctgtgaaga catcaaaaac
     9181 tccaccatag gagcatcatc taccaaaaat ggatagacga gttcctcacc caagatagaa
     9241 ataagatcac taacaagtcc ttctgcttct ccataagttg acgtcaataa cacaatccta
     9301 tcttcttttt ctaaactgct tgcaattgca agagccttag tagatgttga taaaccaagt
     9361 attagttgtc ttttcttatc tgttaaattt tgatgccatt ttttaatctg atcattttct
     9421 gagaataaat ctaataaggt caccatttat ccgttatacc tctgcattgt tttctcaaaa
     9481 tttttctctt gtaaatagta ttttacagaa tcgtcaactt tgtcaacaga ctgtaaaata
     9541 ccgatataat catccctgtc aaacttactc aaaacatgat gaacaactga cataccattt
     9601 ttaggtcttc caattccaat cttaacacgg ttaaagacct gagttcctat atgttgaata
     9661 atagacttga taccattatg accacctgct gagccttttg ctcttaaacg aattttccca
     9721 acttccatgt caagatcatc gtaaatgata agtaaatcgt caatatccaa accatagtaa
     9781 gttaataaag catgaactgc ttttccactt tcattcataa aggtcgttgg tttaaccaga
     9841 taaatttttt ctccatttag gaaaaaggat gctaggtcag cttgaaatat cttatcgtgt
     9901 gtaaaagtga cattctgttt cttcgctagt tgatcaatca acataaaacc aacattgtgt
     9961 tttgtttcaa aatatttatc ccctggattt cccaaaccta caagtaattt ggtcatttat
    10021 ttttcctttc aaaagccaaa agggttggaa tttttttcca acctaattga caccatttat
    10081 taatttttag acattaaagc ggaattctat gatgtcgcca tcttgaacga tatattcttt
    10141 tccttcttca cgcaagcgtc cagcttcttt tacggccttt tcagatccgt atttcactag
    10201 atcttcatat gacatggtta ctgcacgaat aaagcctttt tcaaagtctg agtggataat
    10261 accagctgct tgaggagcct tcataccacg tttgaaagtc caagcgcgaa cttctttttc
    10321 accagctgtg aagtaagttc ccaatccaag caagtggtaa gctgcacgcg tcaacttatc
    10381 tacacctgat tctgtcaaac caatggcttc aagaaactct tttttatctt catcatccaa
    10441 ttcagaaatt tcttcctcag cacgcgcaga aataacgact acttcagcat tttctgtcgc
    10501 tgcaaattca cgaatttgtt tgacatagtc gatagagtca ggttctgaaa ccacatcctc
    10561 gtccacatta gctacataaa gaactggttt agtcgtcaaa aggaaaagac ctttgacaac
    10621 cttttgttcc tcatctgtaa attcaatggt acgagctgat ttcccgtctt ctaggactgg
    10681 tttaatcttt tgaagaacat tgaattctgc tactgattct ttatcttttt gcgtacgtgc
    10741 catcttttct acacgcgcat atcgtttgtt cactgattct aagtcagcaa gaattaattc
    10801 cagattaatt gtatcaatat ctgcaagtgg atctacaaag gcgtcttcac gtccttgctc
    10861 gcgcatcaca ttttcatcat caaaagcacg aactacgtga acaatcgcat ctacttcacg
    10921 aatattggcc aagaatttat tccctagccc ttctcctttt gaagctcctt ttacaatccc
    10981 tgcaatatcc gtaaattcaa atgttgtggg aactgtcttt ttaggagtta tcatttcagt
    11041 tagtttttgt aggcgttcat ctggaacttc caccattcca acatttggat caatcgtcgc
    11101 aaatgggtag tttgctgcct ctgctcctgc ttttgtaatt gcattaaata gtgttgattt
    11161 accaacgttt ggcaaaccaa cgatacctgc tgtcaaagcc atatttctca ttctccgttt
    11221 tcatttcaat ccctaatatt ataacacaaa aagggataaa gtgctaaccc tctaactaag
    11281 ggttctcagt caataatttt attcattttt cgctcaaaat catatcgccc catcatgaca
    11341 acatgctcac aattactgca ttttattttg atatctgctc ctacacgtgt aatttcccaa
    11401 cgattagcct ttttaccagt tgacttgatt gtacaagcgt gtgatttttt catctcaaca
    11461 aaatttccaa cttgatacat actactctcc ttttcttttt cgattatatc ataaaagagg
    11521 cgagccaggc tcaacctctt tcacttaatt tgtacgaact ggtgtaatga gctgcatgaa
    11581 gtcttcgtca gtatctgctg gcacaagagt aaatggacga acagctgaga taaagctaat
    11641 agtcaccttt tcgctattta aagctttaag agaatcaatc aagtaagttg ggttgaaact
    11701 aatggtcaaa tcttcaccag taacctgatc agtatcgatt tcttcgttta ctttaccaac
    11761 ttctggagag tgaacatggg cgctaacaac cccatcctta atttcaagtt tcacagtacc
    11821 attttgagtc gcacttgata aaagacgggc acgctccatt gactggcgta agtttaccac
    11881 attaaaagta atagtagtgt taaagtctgt tggaatcaag cgatctgtat caggatagtt
    11941 tccttctagg agacgagtat agaagctaat attttcgctt ctaaagagga tttggttatt
    12001 ggcaaagaaa atctctacag tttcgatatc atctgtaaat accgctgaaa attcgcgtag
    12061 agaacggcta ggaattacga catcaaaatc atcactattt ttttcaagag tcaatttttt
    12121 ctggcttagg cgatgagagt ctgttgcaac tgtttttaac tctttgtgtt gactcaatac
    12181 gaagtggaca cctgttaaaa tcggacgact ctcttgtgta cttgcagcaa aggctgtttc
    12241 attaataatt ttcttgagta attttgtttc aagtattaaa ggagtgcttg ctgaaatttc
    12301 ttggattcgt ggatattgtt cgctatcttt tccttttagg gtaatttctg atttgccact
    12361 ggttaaaaca atttgatttt gttcaatttc tttaaaatca agagttacat caggtaaact
    12421 agatactaca ttgataaaga aagaagcttc aagaaggatc gaacctaaag aagtaattaa
    12481 caaaccagca tcttcatttt tttgagaaat aaaattttca attgaaattt gaccatttga
    12541 accaattaaa gtaatacctt cattggtcac gtcaattttt actgttgata aaataggaat
    12601 ggcattttta gaactaatag ctctcttagt agtatttaat gcttgtagaa ataaattttt
    12661 attaattgaa aaatgaatca tggattctcc tttatttatt tttagtatta gaaagataat
    12721 agtaataata gagtctgtga aatctgtgga aaacagcctt taaacaagtc atatcaagtt
    12781 ttttagcttg tttaaaaaat gtggataaaa aagataaaaa actaaaagtt atccacaaat
    12841 tatttgattt tctttttgat tgattcaatt tctaaacgta aattatcgtc ttgatcaatc
    12901 aaagatttta ttttggcatg agcatgaatg actgtggtat gatcttttcc cccaaattcc
    12961 ttcccaattt ttggaagact attatctgtt agttctctag ataaatacat ggatacttga
    13021 cgggccaaaa caatattttg aaggcgtcta cttcccttca tttctttgat actaacacca
    13081 taaaagttac caacttcagt ttggatttta tcaattggga tgacgagcat ttggctaaca
    13141 tcttgtttgc gggctctaat ggcttctgca gcaatatcaa tagtgatatc cttgattttt
    13201 tttactctgg caattaaagt gatgtcgttg atggctccct caagatctcg aacatttgaa
    13261 tcaaattgcc cagctaggta ttctagagta tcactttgga aattgtagcc taaatgttcc
    13321 gttttacttt gtaaaatggc aatacgtgtt tcaaagtcag ggggtgtgat agtttgtgtc
    13381 aatccccaac taaaacgcgt gacaagcctc tcctcgagcc cttctagatg ttttggacta
    13441 cgatcactcg ttaggacaat ctgtttttgc ttgtcatgaa gggcgttaaa ggtattgaaa
    13501 aattcttcct gagttgcgac tttttttccg ctgagtgact ggatatcatc gattaacaaa
    13561 agatcaagac tacgataggt ctttttaaac ttttccattt ccccaagtct taggtgatca
    13621 agaaagtcat taataaagct ttcggcaggg atatatttaa cacgcgcatt aggaatattt
    13681 tttagaattt catttccaat agcgtttaat aagtgagtct taccaaggcc tggtcctcca
    13741 tagataaaaa gagggttata ggtcagagcc aaatcttcag agacagctaa agcggctgat
    13801 acagcccaaa catttccatc cccttgaata aagttatcaa aggtatactt ttcttttaat
    13861 cccgtatctg aataaggaat agatactaac tttggactat agtcataaag agttaaattt
    13921 gtagcttctt caacttgtga gctagtcgta tcttgaggtt tggtgaaaat atagtgggga
    13981 gttatttcag cgtcataaat ttcaaaacca gctactacaa taatatcttt tagttgtttt
    14041 tcccagacca tttccatttc agagcgaggt agaaatatag tggcaacatt ttcctctacc
    14101 ttgataagtt cagcttgaat agcatagaaa tcatacatgg atcgagtcag tctttcttgt
    14161 gcaaattcta atatacgatt ccaaaattgt ttttctttca atcctttctc ctcctttact
    14221 atttaaaagt ttaatactag agatatttta ccatagatag caagaatttt ccacaagctg
    14281 tgaaaaataa tccacaatgt taccaaactt tatccacagg ttggggataa aagaagaaat
    14341 tattgattta ttaagctttt tatcgaaaaa catagtggat aaccttgtga gataaaaaaa
    14401 taaaagaaca gccttatttc aggctgttga taattctact atattcttct tgatttgaaa
    14461 aagaaataat gatttttcca ctgtcttttt tagatagttt aatttctaca tctaatccga
    14521 gtagttttct taactgtttt tcttcatttt gtatgaaatg attagttttt tgctgtttct
    14581 tttgtttttt ctctgtcaga agagcttcta atttccttac agaaatatct tcttctataa
    14641 tccgttgaaa gaaatagtct tgttgttcct tatttaaccc aactagggaa cgcgcatggg
    14701 cttgtgatag tttgccattt tctacttctg aaagaatctg ttctggcaag gaaagtaaac
    14761 gaatggagtt gctgatatat ggacgagact tgcccatctt atctgcaatt tcagcatggg
    14821 tgaatccttt ctctacgaga gattcatagg cgcgtgcttc ttctattggg tttaaatttt
    14881 ctctctgtaa attttcaata atggactgga ccatcatctc ttggtctgaa atctgtttaa
    14941 caacagctgg gatagaccgt agaccagcta aaagtgaagc ccgatagcgt ctctctcctg
    15001 caaggatttc ataaccaata acaggagatt gacgaacaat aatcggttga atgaccccat
    15061 tttctttgat agactgtgct agttcatcta gtttttctct atcaaattct tttcggggtt
    15121 gatagggatt tttttgtata tctgtgatag aaatcatttc aaatttttcc atgattctac
    15181 actaacacat cttttctctt atgtaaagct ttctttacat agatgtcaat taagattcta
    15241 aatcacctga actcttgtta agtttgatag aggtagtttc ttctttcccg ttacgatagt
    15301 aggttatctt aatggtgtct ccgatagaat ggttgtaaag agcactttgt aagtctgttg
    15361 atgaagcaat ctctttgtca tctacttttg taattacatc gtatttttca aggtgaccat
    15421 tggcaggcat attactttgt accgaacgaa caattacacc agatgtaaca ttacttggaa
    15481 tattgagtct tctgatgtcg cttgtactca cattagataa attaaccatc tggattccca
    15541 aagctggacg cgtcactttt ccgttttttt ctaactgttc aataatattg atagcatcat
    15601 ttgcaggaat tgcgaaacca agaccttcta cagatgttcc tccatttgta gcaattttac
    15661 ttgaggtaat tccgataacc tgcccttgaa tattgatcag tgggccgcca gagttacctg
    15721 ggttaatagc agtatcagtt tggatggctt ttgtagaaat agcttgtcca tcttccgatt
    15781 ttaaggatac atttctattg agactggata cgataccttg agtgacagta tttgcatatt
    15841 cagaacctaa cgggctaccg atggcaatag cagtttctcc tacagttaac ttactagaat
    15901 caccaaactc agctactgtt gtcacttttt ctgaagagat tttgacgaca gcaatatcag
    15961 agaaagtgtc agctccgaca atttctccag gtactttagt cccatctgac aatcgaatat
    16021 ctactttgct ggcgccattt ataacgtgat tgttggtgac gatgtaagct tctttatcat
    16081 tctttttata aataactcca gatccttcac tagagattcg ctgagaatct gtgtcagtat
    16141 catcattgcc aaatacgcta ttttgtctgt ttgccgaata agtaataaca gaaacaacag
    16201 catcttttac tttgttaaca gcctgtgttg ttgaattttc gttcttatag gcagtttgtg
    16261 taatagtact attgttgtta gagttgttta cactactttt ttgagttagt tgagttattg
    16321 aaaaactacc caaggctcca ctaaaaaagc taatgacgat aacgactaat aattgaaacc
    16381 attttttgta aaatgttttt agatgtttca tatttgcctc catatgtttg aattactgaa
    16441 agtataaact gactagctta attataactt aaacacaaaa gttttacaca aactgtggat
    16501 aactcttttg aaattgtgat tttcttaatt gaaatctatt ttttattttg tgaataagat
    16561 gtgaaaaaat agagaatatg ttagaataga gtcatgaaaa ttaaagttgt aacagttggg
    16621 aaactgaaag aaaagtattt aaaagatggt atcgcagagt attcaaaacg aatttctaga
    16681 tttgctaagt ttgaaatgat tgagttatca gatgaaaaaa caccagataa ggccagtgaa
    16741 tcagaaaatc aaaagatttt agaaatagaa ggtcagagaa ttttatcaaa aattgctgac
    16801 cgtgatttcg ttattgtgtt agccattgaa gggaaaactt tcttctcaga agaatttagt
    16861 aagcagttag aagaaacttc tataaaagga ttttctactc ttacttttat tattggggga
    16921 agtttaggat tatcatcatc tgtaaaaaat agagccaatc tttctgtcag ttttggtcgc
    16981 ctaaccttac cccatcagtt aatgagacta gttcttgttg aacaaatcta tcgcgctttt
    17041 acgattcagc agggattccc ctaccataaa tagaaaattg acttttaatt gaatttttgg
    17101 tagaataatt gtattaggtc tcatagctca gctggataga gcattcgcct tctaagcgaa
    17161 cggtcgcagg ttcgaatcct gctgggatca atataatagc aaagctggga attttcccgg
    17221 cttttttctt aaaaaagtac actttgggag aaaaaaatga cagttgagag aattttatct
    17281 aaaacgaaat tccattttgt ataatggttt ttgtaagtta gcttacaaga aaaaacattt
    17341 taggagattt tattatgaaa aacacagtta aattggaaca gtttgtagct ttgaaggaaa
    17401 aagacttaca aaagattaaa ggtggggaga tgaggatttc aagaatcatc cttgattttc
    17461 ttttcctacg aaaaaagtaa tgaaataagg agaaagagta atggatttac ttggatttgg
    17521 gacagttatt gttcattttt taattattag tcacagttac cgtttaattt gtaaaggtcg
    17581 aataaataga aaagaattat acgtttttgg tgcttataca ttactaactg aaatagtact
    17641 tgaattttcc ttttatcttc tatatttaga taaaataggg attgaaagat ttttatttcc
    17701 tttgggctta tattcctatt ttcgatggat gaaacagtat gagagggata gaggactatt
    17761 cctaagttta ctactatctc ttttatatga gagcactcat aactttctgt ccgtaatttt
    17821 ctcctctata acaggagata attttgtttt acaatatcat ttcccattct ttttcgttgt
    17881 gacggtgtta acctattttg ttacattaaa aatcatttac tatttccatt tggaactagc
    17941 ctattttgac aaggactacc tttatccttt cttgaaaaaa gtattttttg ctttactatt
    18001 gctacatatt gtatctttcg tttcagatat ggtaagtacg attaaacatt tgaatagttt
    18061 tggaagtatt ttgtcatcta ttgtctttat ctctctcctt ttgaccttct ttgcaatgaa
    18121 ttctcataaa gttcaaatgg agaaagagat tgctttgaag cagaagaaat ttgaacagaa
    18181 acatttacag aattacacag atgaaattgt tggtctgtat aatgaaatcc gtggttttcg
    18241 acatgattat gctggaatgc ttgtcagcat gcagatggca attgacagtg gtaatttaca
    18301 gaatttacaa tgaagtttta gtcaaagcaa atcataaatt gcgttcagat aagtacactt
    18361 actttgattt gaacaacata gaagactcag ctttacgaag tttggttgct cagtcaattg
    18421 tctatgctcg aaataatggt gtagagttta cactggaagt aaaagatacg attaccaagc
    18481 ttccaattga actattggat ttggttcgta tcatgagcgt tttattgaat aatgctgtcg
    18541 aaggatcggc tgatagctat aaaaagcaga tggaagtagc agttattaag atggaaactg
    18601 aaacagttat tgtgattcag aattcatgta aaatgacgat gactccttca ggagatctat
    18661 ttgccttagg attctccact aagggaagaa atcgcggagt cggattaaat aatgtgaaag
    18721 aactactaga taagtacaac aatattattt tagaaacaga gatggaaggc agtacattta
    18781 gacaaatcat tagatttaag agggaatttg aatgaaagtt ttaattttag aagatgttat
    18841 tgaacatcaa gtgagactag agagaatatt ggatgaaatt tcgaaagaat cgaatattcc
    18901 aatatcatac aagacaacgg gaaaagtccg tgaatttgaa gaatacattg aaaatgatga
    18961 agtaaatcag ctttatttcc tagatatcga tattcatgga attgagaaaa agggatttga
    19021 agtggctcag ctcattcgtc attacaatcc ttacgctatt atcgtcttta tcactagtcg
    19081 atcagagttt gcgactctaa cctataaata ccaggtatca gccctagatt ttgttgataa
    19141 ggatatcaat gatgagatgt ttaagaagag aattgagcaa aatatcttct acacgaagag
    19201 tatgttactt gaaaatgaag atgttgtaga ttatttcgac tacaattaca agggaaatga
    19261 tttaaaaatt ccttaccatg atattttgta tattgaaaca acaggggtat ctcataaatt
    19321 gcgcattatt ggtaagaatt ttgcaaaaga gttttatggt accatgacag atattcagga
    19381 aaaggacaaa catactcagc gattttattc tcctcacaag tcatttttgg taaatatagg
    19441 taatatcaga gaaattgatc gaaaaaactt agaaattgtt ttctatgaag accatcgttg
    19501 tcctatttca agattaaaaa ttagaaaatt aaaagatatt ttagagaaaa aatctcaaaa
    19561 gtgattgaca attagcaaga aattgatata atggttatat ctgctacaga tagaaggtcc
    19621 gttggtcaag gggttaagac accgcctttt cacggcggta acacgggttc gaatcccgta
    19681 cggactattt tatccgccat agctcagttg gtagtagcgc atgactgtta atcatgatgt
    19741 cgtaggttcg agtcctactg gcggagtata gataaaaggg aacacagctg tgttcctctt
    19801 tttgtatcaa tttgtatcac caagcatttt cataaggaag tctgttattt cttgagaact
    19861 ttcttttttt ccatgtgcaa tccaagtttg gcagacacca aaaagtgcat gagttagata
    19921 gatgctacta tattctaatt cagtggtatt tagattcagt tgcataaatc gcttttgtaa
    19981 atctgtacta agcatgatat gaagtttatt tcgtaagaaa ttttggattt ctttagtccc
    20041 attttcagaa agaagggcag ccagaagtgg ttctgactct agatattcaa aaacttctaa
    20101 aatagcgtct cttttgtgat gagcatgttt ttgaaaaata tattcaaatg tatggaatag
    20161 cttgctttga tagtgctcaa tcatatcata cttatcctta tagtgagtat agaagctgga
    20221 acgactaatt ccggcttttt ctactaattt gacagtagaa attttatcaa atggctgttc
    20281 catcagtaat tgtaccatag cattttcaat agttcgcttt gtttttaagc gtttgttact
    20341 ttcttgcata tttcctcctt gtaaacaaat tagactatat gtctaaaaat agatttttta
    20401 tcttgtaatt tagatttttt aatgtataat ctattatatc aaaattttag acaatatgtt
    20461 taaaaaagga gaaactaagt ttaaagaatg gaaagcaatt taaaaaaaac caacctttat
    20521 tattgtcatg atcgggattt ctcttattcc agatctgtac aatatcatat ttttgtcatc
    20581 aatgtgggat ccatatgggc aattgtctga cttacctgtg gcagttgtaa ataatgataa
    20641 agaggcttcc tataatggta atactatggc aataggaaaa gacatggtgt ccaatttaaa
    20701 agaaaataaa accttggatt ttcattttgt agatgaagag gaaggaaaga agggattgga
    20761 agatggcgat tactatatgg tagtgacttt accaagtgat ttatctgaaa aaacaactac
    20821 attatccaat attcaatcga cagcagctta tcaatcattg acaagtgagc aacaaactga
    20881 gataagtgat tctgtatctc aaaattcaac tgatagtatt caatcggctc agtcaattgt
    20941 agctttagta caagatttac agggaagttt agaaaactta caaaatcaat cttctaatct
    21001 ttcgacttta aaaaatcaat ctaatcaagt atcacctatt acttctactt ctttgatagg
    21061 attgtcaagt ggattaacag agatacaagg agatgttact agcaaattag ttcctgccag
    21121 tcagtcgatt gcatcaggtg taaacgcata tactacaggt gttgataaag tttctcaggg
    21181 cgcaagtcaa ctaagtgaaa aaaatgccac cttgacaggt agtttggata aactagtttc
    21241 aggctcaaac accttgacac aaaaatcttc tagattgaca gcaggagttg gttaattaca
    21301 atcaggatct gggcaattag cagacaaatc cagtcagtta ctttcaggtg cttctccatt
    21361 agagaataga gctaataaat tggcagatgg atctgggaaa ctagcagaag gtggaacaaa
    21421 gttaacttct ggattggaag atttacagac aggacttgct tctttaggac aaggactagg
    21481 taatgctagt gatcaactca aatcagtatc aacagaatct aaaaatgcag agattttgtc
    21541 aaatccactc aatctttcaa aaacagacaa tgatcaagtt cctgtaaatg gaatcgcaat
    21601 agctccttat atgatatcag ttgctctttt tgttgcagca atatcaacaa atatgatatt
    21661 tgcgaaattg ccttcaggac gtcatccaga gagccgttgg gcttggttga aatctcgagc
    21721 tgaaataaat ggtattatag ctgttttggc aggaattttg gtatatggag gagttcagct
    21781 tattggttta actgctaatc atgagatgag aatatttatt ctcatcatcc taacaagttt
    21841 agtattcatg tctatggtga ccactttaac aacgtggaat agccgtatag gagctttttt
    21901 cttacttatt ttgcttttac tacagttagc atcaagtgca ggtacttatc cacttgcttt
    21961 gacaaatgat ttctttagat ctattaatcc ctggttacca atgagctatt cagtttcggg
    22021 attacgacaa acaatctcta tcaacaagtc attttcctag ctgtcatact agctctattt
    22081 actggtttag gtatgctagc ctatcaacat aagaaaatgg aagaagatta aaaaaatcga
    22141 ccgattaatg aactgcaccc caaaagttag acagaaaaaa tctaactttt gaggt
//
LOCUS       DAVBCG010000019        21477 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBCG010000019 DAVBCG010000000
VERSION     DAVBCG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.019"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(46..270)
                     /locus_tag="VQ495_000627"
     CDS             complement(46..270)
                     /locus_tag="VQ495_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HET2530617.1"
                     /translation="MIEMLIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(275..454)
                     /gene="asp4"
                     /locus_tag="VQ495_000628"
     CDS             complement(275..454)
                     /gene="asp4"
                     /locus_tag="VQ495_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HET2530618.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(456..1793)
                     /gene="gtfB"
                     /locus_tag="VQ495_000629"
     CDS             complement(456..1793)
                     /gene="gtfB"
                     /locus_tag="VQ495_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HET2530619.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPEH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQEIMQTVLGG"
     gene            complement(1786..3297)
                     /gene="gtfA"
                     /locus_tag="VQ495_000630"
     CDS             complement(1786..3297)
                     /gene="gtfA"
                     /locus_tag="VQ495_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HET2530620.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3309..5681)
                     /gene="secA2"
                     /locus_tag="VQ495_000631"
     CDS             complement(3309..5681)
                     /gene="secA2"
                     /locus_tag="VQ495_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET2530621.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSSLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5674..6114)
                     /gene="asp3"
                     /locus_tag="VQ495_000632"
     CDS             complement(5674..6114)
                     /gene="asp3"
                     /locus_tag="VQ495_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HET2530622.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6111..7646)
                     /gene="asp2"
                     /locus_tag="VQ495_000633"
     CDS             complement(6111..7646)
                     /gene="asp2"
                     /locus_tag="VQ495_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HET2530623.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7630..9210)
                     /gene="asp1"
                     /locus_tag="VQ495_000634"
     CDS             complement(7630..9210)
                     /gene="asp1"
                     /locus_tag="VQ495_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HET2530624.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9220..10437)
                     /gene="secY2"
                     /locus_tag="VQ495_000635"
     CDS             complement(9220..10437)
                     /gene="secY2"
                     /locus_tag="VQ495_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HET2530625.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYFYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            complement(10494..11171)
                     /locus_tag="VQ495_000636"
     CDS             complement(10494..11171)
                     /locus_tag="VQ495_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2530626.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11156..11401)
                     /locus_tag="VQ495_000637"
     CDS             complement(11156..11401)
                     /locus_tag="VQ495_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2530627.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGGQHKLKIWGLV"
     gene            complement(11401..12597)
                     /locus_tag="VQ495_000638"
     CDS             complement(11401..12597)
                     /locus_tag="VQ495_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2530628.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(12606..13826)
                     /locus_tag="VQ495_000639"
     CDS             complement(12606..13826)
                     /locus_tag="VQ495_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET2530629.1"
                     /translation="MRNTKRAVAFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13857..16301)
                     /locus_tag="VQ495_000640"
     CDS             complement(13857..16301)
                     /locus_tag="VQ495_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HET2530630.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELDKEFNLQVGHDVTAFYNKWENYFNE
                     LVPPSIIHFVSYRKPWTTLIANRYRDLWWEFHDLEWTKILQHHIGEFELTSSLDKEFS
                     CLTLTNSQDLEGIEELVTALPDVVFHIAAWTDMGDKLIKLAVYDNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHEIEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(16311..17321)
                     /locus_tag="VQ495_000641"
     CDS             complement(16311..17321)
                     /locus_tag="VQ495_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2530631.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTYQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(17350..18225)
                     /locus_tag="VQ495_000642"
     CDS             complement(17350..18225)
                     /locus_tag="VQ495_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2530632.1"
                     /translation="MENELISIVVPIYNVENYLRQCLDSIQKQTYPNFECIMVNDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(18265..19143)
                     /locus_tag="VQ495_000643"
     CDS             complement(18265..19143)
                     /locus_tag="VQ495_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HET2530633.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(19140..21225)
                     /locus_tag="VQ495_000644"
                     /pseudo
     CDS             complement(19140..21225)
                     /locus_tag="VQ495_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(21286..>21477)
                     /locus_tag="VQ495_000645"
     CDS             complement(21286..>21477)
                     /locus_tag="VQ495_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2530634.1"
                     /translation="SVSNSANHSNSQVGNASGSTGKSQKELPNTGTESSIGSVLLGVL
                     AAVTGIGLVAKRRKRDEEE"
BASE COUNT         6908 a         4578 c         3273 g         6718 t
ORIGIN      
        1 aaacttcaga ccaatccaca aaaggtgaat atctgaagtt ttcttttatt tataagtaat
       61 caccataaag gttaatagta gaacaaataa agccaaactc accaataaag tgagcacctt
      121 gaccaaggtg ttgctctgcc agtagcttgg tttaccaata ttactagtgg catccatgga
      181 aaatagttga ttttgacggg gttgtatagt taccaataca atcaaagcaa gagataggat
      241 aatagctaaa acaattagca tttcaatcat tgatctacct caaaattccc aacaaagtaa
      301 agagcaaacc aatcagaatc attaagccca gtaaaagtga aaaagtttta ctaatcttct
      361 cccctcgggt ttctttttcc ttcttgatgg gtttttgttt caactcttcc atgcgacctt
      421 caacgtcttt gtaaaataaa tcttcctctg acatgttagc ctcctaaaac agtttgcatt
      481 atttcttgat atcgcaccaa gtcaacataa tttgcatgtt ggccttgttt gccaagtgcc
      541 tgacgcattt gatcaacatc tgaaagggcc aatttaatgg tctcaaccaa ggcagcaact
      601 tcactacttt caaatagatg ttctggagcg atataaagtc tattgtgaac cgtttggtta
      661 aatccaagaa tcaagagatt gtgctcaaag gcctgacgca ctgcctgcaa caactcattg
      721 ctgtgattga tatccaagta aatatccgac agttgataca gctcctgaat cttctgtgga
      781 ctagcattct ggtaaaggac cacattagga tagcgaagca tgtctaagag cttagaagac
      841 atctctgtca ctgctgcgat acggaaggta acatcaggca aggcttctac gattgcttct
      901 acttgctcaa tctgatctga attggtcaag attaaggcat ctcgtcttag gaaattatca
      961 cgtttgaact ggtaatgata acccaagtgc acaaactgat catggtattt ctcgtcagtt
     1021 aactctaaag cgcgctcata agtcgcctta tttggaatga tgatcttctt agtacgcaca
     1081 ttatcacttt ccaaaatcaa ctgcatatta cctggaatgg catcatagag aggttcctgc
     1141 cataccaaga catccgagcc agatttatct ggatgatgga aggaaaccaa gaaaggagtc
     1201 gctagagtat taaagataag ctgactggta tctatttcca aatcttgcaa aaagaaggtg
     1261 ataaattcaa ctttatttgc aaagtaacgc atggactgac caggcaaagt caataagata
     1321 tcacccgtca catggttttc cagtaaaact tgttggccat tgacatcttg gtaaactgtc
     1381 ataatcggct cgctatctgc gctataagtg gttttagcaa agcaagcacc aaagcgattg
     1441 tagtggtcaa cctgacgtac tcgaccttct aggtctttcc agtctacctg tttaaccaag
     1501 cgagcctgca ttccatcaac ataatgaatg acagccctct cctgcgtcac atcttcaata
     1561 caagcagact gattatctcc taaaatttcc caaaaatctg aaacgggaac ttggttaaaa
     1621 tagagaggtt ttccatcctc gtaacctaga taataggtaa aaggagaaag cagaccatca
     1681 ggcagaaaac catctgcatc gatgaccact ccaagttgag aaagaccagt agcgactaga
     1741 ctttcatgta aatctctact ttcttgactg taactatcat aaagttcaat catggagcac
     1801 ctcctctact gttttcttcc acttttctaa aatttctttg gtcaagaagc cttctgcaat
     1861 ttgataagaa taggcacgca tagcttccaa acgattttct tgatacaatt gacaaatctt
     1921 agcggcataa gcttgcttga tttggtcttc tacatggtca gatgaacttg gaatcaaata
     1981 accattttgc ccatcctcta taaaggtctg attaccataa ggcacatcaa aaccaattag
     2041 aggtagacct gaaccaatag cttccatcaa ggtcagacca aatccttcgc tggtagaagc
     2101 cgttaagtag acctcatact ggctataaat ctgcgaaagt tccgcatgcc ccttgagttg
     2161 gatatagtcc tctgcctgat gatttgcaat aatttctcta agcagagaat cttctccacc
     2221 actaccatag atatcaaagg ttagttccgg taactcctta tgagcttcaa tcacagcttt
     2281 cacaagccaa tcaatgtgct tttctttggc aagacgtgaa gccgtaatca atgaaaatgg
     2341 tttgcgccct tgacttgaat ctgtcaagga atcaatactg cctacaggaa tggtaacaat
     2401 ctttggctga tgctgagtat atttggcaaa ttgctcttgt agaacttcat tttgtctatc
     2461 agtagacacg ataaagaagt caaccttatc tgcattggta aactgatagt cataatagtt
     2521 attccaaagg atatagtcct catttgtagc attttcacta taatgctccg catgaacaac
     2581 taccgctaga tgtgctgtct gtgcttcctc aaacacaacc tgtccaatac ctgtctccct
     2641 atccagaata accaaatcag acttgttcaa attcaaagat ttcataaagg cacgcacaaa
     2701 agcttgcttt ccatagaaaa tcttatcctt gaaatgataa acttcttcct tcccttgatt
     2761 catcaaaata tcatagactg gagtcccgtc ttcattgtaa aaagttcgtt ggtataagac
     2821 tgcaacattg tccttgggag caaaatactc gctacaataa cgcgtataag aaaagtaatc
     2881 cttccgaatc aggtttccct taaaaacata ctcggcatgt tgaaccaagt ccttgttctc
     2941 atcaaccaaa taacaggtta caaacttatc ttggtcaaaa aagaatacac gtaaaacctt
     3001 gccatttttt tctctgtgac tttcttcacc accaaagtaa gccaagacat catccactgt
     3061 cacgctagta ggtgcaattt tgatatctgt gaaatgatta taaagccaga taacctgatt
     3121 atcatcaaaa ccaatattgg ctgttaagtg ctgaatatta tcggctaaaa tcatatctgt
     3181 aaagataaac ttagaggaca gatttaattt ccgaaaaaca ccagcacgat aggcttgagc
     3241 gtattcaaca ccgctactag cccaaccaat tcctaaattt atattgtaaa ttgtcatatt
     3301 ttctcctctt atggaaaatg agtcacgatt tcaccttttg gagtgacctc aaccagcccc
     3361 atcaggagat tacgcaccat atccgcacgg atttgttctt tcatagcttc aaagcccgcg
     3421 taggcttctt gatagtactc tacgattgga tttttctgac tagcagattg accaccgata
     3481 gccatggata gctgttgtag atagtctacc tgctctaccc agttgtcatc aatggcttta
     3541 agcagtgaaa gtcgtaaaaa ctgttcatat aagtcatgtt gattaagtaa ttctttcttt
     3601 tcagaaagtt ctttatcaat cacctgcttc ataaagctac gaactgcagt tttgtcagtt
     3661 acatctatat aatctggaac ctctttaaca tgaaaactaa tattggtcac aataaagtga
     3721 aacaataatt cacgactagc atagtgatca gccgctacct cttctgtata tctctcaatg
     3781 atatccacaa caacatcctc taagtcacga gaaccatcta ttagacgatt tctctcttta
     3841 tagactatat cccgttgtat attcatactt tcagcatact ccagagtctg acgacgtgct
     3901 gaacgtccag cactatcact ggcatgctga gccttttcga ctagtttccg gtatttacga
     3961 cctttcaata cttccggttg agtcatatct tgaacctgat agtctttgta ctttttatgc
     4021 acccaagatg gaccaaattt cttgataaca tcatcctcta aggatacaaa aaatttactc
     4081 ataccaggat ctccctgacg accagaacgg ccacgaattt gtaggtcgat ccgctgactt
     4141 tccatccgct cagtcccaat aacaatcaag cccccaagct ctgcgactcc tttaccaagc
     4201 ttgatatccg taccacgtcc tgccatagag gtagccactg tcacagcccc catctgacct
     4261 gactcggaga taatctgagc ctcacgcgcc gcattattag catttaggac attatgggca
     4321 atcccttcac gaaacaagag agacgaatag agttgagaca tttcaactga gcctacaaaa
     4381 acgagtaaag gatttccctt agcatggtat tgcttgatgt actccaagga tgcatacact
     4441 ttttcaggta aagtgatata tagattatct ggatagtcaa tccgttgtct cggacgattg
     4501 gttggaatgc gtactacaga catattgtaa gtttcaataa actctttttc cgcgacctta
     4561 cctgtccctg tcataccaga tatcttatta aacatcttaa aaagactctg ataggtgatc
     4621 gaggccatag cccgcgtctc aggagataat ttgacatgtt ccttggcttc aatagcctga
     4681 tggagacctc cttgaagttt agtcatttcc attagacgcc ctgttccctt atcaaccagt
     4741 accatctcat ttccacgaat gatatagtcc ttatctttag taaagagctt atgagctcga
     4801 atcgcataaa ccaaatgacg agcaaaagac gcatgctctt ccttgtataa attatcaatc
     4861 cctaggaaat tctcagcaga cttggccccc ttagtagtga gccaaacctc ctctttctcc
     4921 tctttaaaga tataatcctc tccttcgacc aaggttgtta caagtgtatc aatgatcgca
     4981 tagtaattag actgaacacg aggagaaccc gcaataatca gaggagtttg tgcactatca
     5041 agcaagatat catcaatttc atcaataata acatagttaa acggtcgtaa aaacttacct
     5101 tcttcattcg aggctaggtt atcatttaga taatcaaaac ctaaactact attggttgtg
     5161 tagatgatat ccgaagcata gataagcttc ttttcttcag ctttcatttc cttctttgga
     5221 tcttccgtaa atggtacacc aatggtcaat cctagaaaac gataaacttg gcccatttcc
     5281 tcggcatcac gctttgataa atactcatta ggagtcacaa ccatcactcc ttctcctgaa
     5341 aaagcgttca aatagacagg catggtagct gtcaaggtct taccttcccc cgtattcatc
     5401 tcagcaacat ttccatagtg catgacaata gctcccatga cttgaacatc ataggggaac
     5461 atccctaaaa tccgcttatc tgcttcacgc accacagcaa aagcttcaac caaaatatcg
     5521 tctagacttt ctccctcgga aagacgctga cgaaactcta ctgttttagc tactaattct
     5581 tgatccgaaa gagaggacat cttgcctttc agggcattaa tctgctttaa aatctttttc
     5641 actttcctaa gctggaaatc ttgacctaaa cgtctaaaca cttctgatct ccttgataga
     5701 aaaatgataa aaagttaagg actcaaatcc agcacttaaa agagaaactt gataagtata
     5761 actatcttct ggataagtaa atatgaaatc tttcgctttt tctacctttt tttctaaaat
     5821 atcaccatag cggtcaaaaa aagttatctt ggtataaact ccattttcag gctgacaatc
     5881 aaaaaccagt gatagttgat actcctgctt tctttttaat aagggaagct gaggttgggt
     5941 tcgtgcagcc tggtaattga cactagaaaa ccaagtcttt agaatctcac cagatggcaa
     6001 tagaggatta tacagacgaa cacttttgtc aaggtaataa gatacagttg ttccatacat
     6061 ataagtccca ccaacttctc cccaataaat agactgtctc tgtgtaataa tcatcttttc
     6121 tcccttccaa aaccagtctc taagaccatt ctataaaagt gcaaaaacca agaaatattg
     6181 gtatctgtat catcattgtg tcgtccagca gttcctttag ataaaatctt cgcacctgta
     6241 ttacacagat aagacactaa ttgttcatag gcttgtggat ccatttcttc atctttcata
     6301 taggataaac caaaggttgt ctttgaaaaa tttgcttgtt taaaaatttt ccaaaaacgc
     6361 tgatccaact ccgtcatatc ttgagaactt gtcccacctg tatgatgaat taaacaatca
     6421 aaagctaagt tagagacacc cggtgcgtcc aaacgtccac gactagctat agttcctaaa
     6481 ttagccaagg gcttaccaac tatgatggca tggggttcaa aagaagcacc ataatagaga
     6541 gccggaaacg ttcccataga aagacctgat aaaatcaaat ccttatgatc aaagcctaaa
     6601 taatcaagat agtgagtgat cgtatccttt acttttccct ctagctcatc cgttccgaga
     6661 taaaaagctc ccccctctaa acgtggatca gaaaataaaa tgaagggaca tccaagagtt
     6721 ttcatcataa agtaaccctc gaatccttct gcaggtcgat aacctgcaaa atagacagtc
     6781 aaaggcggtt taaaatcacc tggatggaag aaatagttta tttcatcacg cttggaatca
     6841 tgtaggatat ttccaccaag tacaaactta ccaaattgtt ttcgactcca tcgttggtga
     6901 agattaccta gtttgatagt tccacgacct cttgcttcaa cagatatcga gagaaagtag
     6961 tctccgtctc gactatccat aaaaagtgct tgctccaagt ctttttcaga tactattact
     7021 tcttcaaaga aatcatcaac agacccactc cacattttac ggattactaa acgaaagtca
     7081 caattgcctt ccttctcata ttcaagccac aactcaatag ggagtgtttt ttgcaccata
     7141 atattataag cccaataggc aagctgggca aaatcttgcc caaactcacc ttccaaactg
     7201 acataatcca atccttgata agaaatagct cctgtaaaat ttggattgac ttgtatcgtc
     7261 ggaggaaaga gtttatcacc atacccaccg ccaaaaagag atttacttaa atcattgatt
     7321 agttgttggg gacttaaaaa atcaattgct tgtgcacatc gtttttttag aaaatccaca
     7381 acctctctat catttatttg tagattctgg ttatagaaca aagtataagg ttcaaaatat
     7441 tttacatagg aaaataagtc tctggaatac tgaccatctt ctattaaaac tgcatgaaaa
     7501 cggttaatat cctctttttc gattatttca cgaagggttt ttgatgagtt tgggtaaaaa
     7561 taataccaac tcatattttc aggtatgtca tagtgatttt cccaattggc aagtcctatc
     7621 tgtaaaatat taagttcctt tgacattttt aacctcctct atccatttct ctaattttcc
     7681 aagaaattgt tgtcctgtgt gctccttaat cttatcaatt gaatatatca aggactcatt
     7741 ccactccttc aacctatctg tgtaataatg tgcagcctta gaaaattctg tcacatctgt
     7801 cagcaaataa ccatttttta aatgttccac atagacggtt tcaactaggt tgatttgagg
     7861 aattcctgca ctaatcccag caatctgtgt gtagagatga ggatgtctat ttaaatccac
     7921 aatcaaacgg acaaattcta gtgtttttat caaatctaac tcatcattca agttaacaaa
     7981 ctgtaagcgt aagtcctggt gttgattttc ttccagggga ttttctgcat caccataatc
     8041 aatcgccttt cccagatttt cggattgaat gttttcttgg atgaaagact caacaatccc
     8101 ttcaacctca ttcatttgct cctgactagc agcaaaggct ccaaaaatca cctcagtatc
     8161 cttattttca gcgacaaagg acaagacttg aagcagagct tgattatcaa tcccctgctc
     8221 aaaatccagt tgaaaataaa tgatggattc tttcttagtt tggcttcgtc ccaatcgtaa
     8281 tcgggtgtca aaagatgata aatgataaca ttgatgcatt cgttcaggat acaactcctg
     8341 caacaatcgt aaactatcct ctctatccac caaaatcaaa tccgattttt caaaagttac
     8401 atctaaatcc ctaaaggtat cttgaggatt acgtccaatg aacagactca acagtttagg
     8461 attagtacta ggtaaacgat ctagtaccaa ctggtcatga tgagaatgag aaggaagcat
     8521 aaaaatatcc tgatccttca catacgtttg cagataattc tcaaaaaatt ctgccaccag
     8581 atctcccata ttttgatagg taagcatttt aaagcgataa cccaaaattg gattgacttc
     8641 tacctgtcct ccttctttta aacgctctct aaattgccaa actcccttag gattcaaata
     8701 ttcttgatag gctgcttgcc catcttcaaa aaaaataata ctcgatataa accctcgatc
     8761 atccatgata tggtgcatat gtatttgacc atttggttca aaatactgta tatcactgat
     8821 gaagccttca actccatgtt caaccttagc aaatttcttc ccattttttt gaacgataat
     8881 cgtaaaggga ctataaataa attcacagtc atcatcccac tcaatatctc taatgcttaa
     8941 aacttgggta tggagattat gaaaatcttg cataacatca aaaacggaat aagtatccgt
     9001 ttctaacaca ccatgtctat ataaaaaata acgtagatga ggctggtaag ctaatactaa
     9061 tagacgagaa tctatatctt gctcttgaaa gagccggatc tggtgaaagg tatcatcaaa
     9121 ctctagacga aaatgagaaa aataccatgg agtgatatct gcatgccatg ttctttctga
     9181 cccataccaa gctggaataa aataatacat aaaagtctcc taaatcaaag gtttataggt
     9241 ctcatttagc cttaaagcct tgacctcgtc tctaatcgta aaaatcatgc ctccaaacat
     9301 cataaacaaa cctggaatca ttgccaatcg taataacttt tcatcaaaca aaacaaaaag
     9361 catgggacca cctgccataa ccacattaaa gagaccacct atgactgaga aacgaaggac
     9421 caatcgatta ataaatcgac tagtatccgc acctggataa ataccataaa tgtattctcc
     9481 agattttttc atacggtctg caatctcttc tccattcatc gtaacaaaag caaaaatgat
     9541 actaaataaa aataagaccg aaatataaac atagacccac aaaggctttc caatcataaa
     9601 ttcctttgat aaagccgcta accctgaatg attagggaaa ataaatccca acaagatgaa
     9661 caaataagct ggtacactaa gaaaactcat cacatacata taaggcatcc cacctgcagg
     9721 attcaacatg atttcgagat aaaaatagcg tttaaatcga ttgtgtaagc caattttatt
     9781 aacaggaacc aaatagcgag ctcgatacat aagggcaagt aaataagaaa agacaagggt
     9841 taataaagca agtaacacaa taatccctgt tggaatgtgt actgtctgaa atgtttccaa
     9901 aacatcctga ggaatattta aaaccatact ggataggaga atgactatag aacctccaat
     9961 ccccatactc gcatttaaat ctgacaacca aacaagaaaa aatgttcctg ctatcagcaa
    10021 tattgtattc attagaacaa ccaatattgc agaataggag gattgtactg gcagtctcaa
    10081 gctaactgcc aatgattgaa tgacagctat catcaaggtc aggtacattt tacggcgatc
    10141 ttgtatttct atagacgtag atgttaaacc caaccgttta gaaaaagaaa acatctgcca
    10201 taaaatcata gctgacatcc aaggggacaa tccaacagaa aaaattgaca aacttcttag
    10261 atttccacct gttagggcgg ctgagaaggc tagataggct gttgaacctc ctaaaaaatc
    10321 tttagtattt aggtcaacaa aagggagagt aatacgactt cccaatacat agataaacag
    10381 tagaaataag gtaaatagtc ctttttttac tgctattgac gaataaatct tagtcataaa
    10441 acactcccct caccttcatt gtttgtgata tggtaaataa caattctttt atttcaactt
    10501 ttaccatttc tttgttctgc atcaaaaaac aactttaatc tgaaacaaat atctttatat
    10561 tcctcagtat ctgttaattg atggtattct aaagtttgct tataatcttt taaaagattg
    10621 acaaatcgaa tacgcagaac ggagacatcc aaaccagcca agacacaatc cgatattttc
    10681 ttagaaaaca cttcaacaat atctcttgcc caggaaagat caaattccgt tctcatgatg
    10741 cttcctgaac gtcttctata cagatagaga ttatcgttta taaagacgat tttagaggct
    10801 aaaagataaa agcgatgcat agttgcttca tcatcaaagc ggcgaccatt tgaaaagtga
    10861 acttcattga ataattcttt tttaatcaac ttaaatgtcg gcaatataaa ggccgagcta
    10921 ttgaatttcc aatctcctgc ttgacggttc ataatttctt gtatagctaa ttcttcgacg
    10981 caaaaatctt gccctgttac ataaaaataa aaattcccgt cactttcgtc ataattatag
    11041 taattcccta taacaacatc tgcatccttt tcttggactt gctgataaag agtttcaatc
    11101 atattttctt ttacgatatc atctgaatca acaatcgtga tgtactctcc cttagctaca
    11161 ctaatcccca tatttttagc ttgtgctgcc cccccccctt attttcttga tgcaatactc
    11221 gcacacgatt atcttgcatc ataaaagcat cacaaatttc cccagaatta tccgtagaac
    11281 cgtcatcaac taaaataatt tctatatttt gataggtttg cttcagaata tgctcgacac
    11341 actccactaa atattttccc gtattgtata tcggtaccac aacactaatt aattcactca
    11401 ttactctatc tctatattct tcattctatc cactaaatca tctggtctct ccacagaaaa
    11461 aataccgtca tagagtccat catccatact tttacgtgtg atatcaaaag cgaagatttt
    11521 cttgccactt tcttgtgccc ttgtaactac attaaaaacc tctccacctg tattaatatc
    11581 taaataaact atagaatcgt ccaatagcca atcaattcta ctatgtaata cattttgata
    11641 tactgttaca ttcgtatact gtgatagaga ggtcagctcc tcagaacaat cacacggtgc
    11701 agccaaatga aaatgccaat caggtaaccg ttgtactaaa tactctaaat gttttatatc
    11761 tgcactccat gtcacaagca tcacttgttt tttagactga ttgcttcttt caaaataatt
    11821 taaatcggaa cgttgaaaag caatctctga ccaatccaaa tctctataaa cccaccataa
    11881 ttcacgtagt ctagatatac tatatgtatt ccaaggttta tcatgactag catagtgaac
    11941 aattgtaggt ggattgtcat ctagacgttc acattcttga gcaaggtgat aaatatcaac
    12001 acccaccata taattatatg ttttatctaa ggctagccaa ttatcctcaa aataaatatt
    12061 taaaatactc tgatccccaa gatgaacaac ttgattttgc tcggccgcta attccaataa
    12121 actattgaca atagagtgtt ctttccattt tgcaacatcc attagtaaca taccagcatt
    12181 aaatccagat tttcgtcctt cataggcata gacatcatca acagcaccaa ttgaatatcc
    12241 tttgagatct atctcaaaca aagtagctag ttccccagtt acaatgatat cggaatccag
    12301 atacaatacc ctatcagatt caaccacttc tgtcgcaaaa aatctaaagt aagaagcata
    12361 atttatatga ggtcctgttt tatagctttc aaaaagttct ttatcaatgt gtacactgcg
    12421 aattgtagaa ttcacaactt ccatttttct ccccaataat ttaaaccatt caggagctat
    12481 atcactgttg agaatataaa aatcaacatc tctattgtga tacaatacag actttatagt
    12541 cgtctctaaa ggaataagat aggcattatc tgccgctaat actattgatt ttctcatact
    12601 gttctctacc tcatgtaaga tctgattgct gcaaccattt ttttcggaat ggagcttgga
    12661 tagatacctt catatgaaat ctcagaaaga ttttcagagc aagtattgtc gaaagctatc
    12721 attggttttt tgtacttaag cacaaactca tatgcatctt ctaattttcg atcatgattc
    12781 aaatccaaat acaaatctga agctattact atttgttcca ataacattgg taaggaattt
    12841 ggatatacgg taacattagg aaaacgcgtt aaagctagta gttcatttgc catatttgta
    12901 taagcaacaa tatgaaaatg aacttctggc aattgctccg ccaaataatc gattttctcg
    12961 acacaccaag aattcgttaa attaacacat tgtaacttaa atatttgtga tttactagga
    13021 tacttcacac tctttgaaaa ccattcattt aaaataacag accaatccat caaagagtat
    13081 tcccaccaaa cttctcttag acgtccaaca gaaaattgat tccaaggctt atcctgagaa
    13141 atatagtgta aaattagtgg cagtggttcg agcggaatat caaaaatgaa ttgatgttta
    13201 aaggctgccg ccccataatc atatcctatt tgaaaattat attgatcttc aagggaacta
    13261 tattgatctt taaacaacat attcaaaatt gactggtctc cttcttccac attctcatgt
    13321 tctttttctg ttaagtcaat caatttttgt cgaatagttt cagatcccca ttttttgttg
    13381 ttaatcaaga gaacaccagc attgaagccg actcctgctc caaagcaaga acgagctgct
    13441 gccaaataat tttcacctaa gtctaattca aacaaatcgg tcaaatcacc agtcacaatc
    13501 aaatcactat ctagatagag aactttatct tctgttacaa aatctggaat aaaatagcgt
    13561 gcaaatgtca tatgattgat atgaggtaat ttattagacc aattcatttg aaactgtgag
    13621 ccaattaact tgcagtcaat caagtcgccc cccatctctt gtaaatatat tcttatttga
    13681 ctaaaccatt cctgaggaat gtcctgattt agcagataaa ttttcaaatg actattgtgt
    13741 ctacagagtg acttcatcgc cgtttcgatt tgtcgaatat aagcgtaatc acctgcaaat
    13801 gctacagcgc gtttggtatt tctcatttta aatccttatc atttatttta aatagtctag
    13861 tttaatattc gtgcaacaat ctgactgtta taggtttcat catcaataaa ttcaatatct
    13921 tgatcaaaat tatactctgc agtatgttta tgagaaaatt taaccttagt tttagctccc
    13981 attttcatcc attcatactc tgagtcaata tggcctacat ccaaaacttg atagcccatc
    14041 tggcatagat tataactcag aacttttgct gtaggaccca gcatacataa aatcaagcga
    14101 ccaccagcat acttttcaat ttcttgttca atttcatgaa ctctagaaaa ggcactatga
    14161 gaaggacaga taattcgctt aatagaattt gcctcatcga ataaatcatt tccgacacct
    14221 gaacgagaag tcgcaccttc gactatcagt aagtcacggt tttcccaaat gcttttcaat
    14281 ttttcaaatt gagctttagc ttgactcttg tcttcaaaat cgatataagg gcgagataca
    14341 aaggttgagc cataccatga atcactgcat aactctctgt aaaaatccat ataatgatcc
    14401 atgtgatctt tccaaaatgg aatcgcccac gatgtaaacc taaaacgatc tgtaaaagca
    14461 tcaggaaggc acactactaa atcttctcga ctttcttggc cgataatgtc cctcatgatt
    14521 gaaaccaact cttcatcata atcctggtag ggaattgaat gccctgcaag catattgatt
    14581 tccccatctc caaaacgaac taaagaagag ttgtgttcaa taatataatc caaggtttca
    14641 tctatccctt tcacctggat tatatcaagc ggtttttcta gcatatcctt agttatcaat
    14701 ttttgcaaat cagccaacat tcgttcagga tgttcacttg aatacaacct ttggccatta
    14761 ttcccatttt gagatttata aaaggctagt ataggtttag atagatgagc catttctgca
    14821 acgatatcgg atgttgaagt cagtaggttg atatctaaat aagcatccat cttctccttc
    14881 aactcgtcta gcttatctct agaaattgcc ggatagagct gaatattagg ataacgatta
    14941 gacaaatcat ataattttgg ccccatagct gtccaagcag caacttgaaa aacaacattt
    15001 ggcaactgtc cagccaagta atccaattgt tcgatatcct gagaagccgt aaacgttaaa
    15061 caagttaaac ttggaagttg tcgaaaacag gtaagatgtc cgtttttctt aaaatcttta
    15121 atcgtatcaa tcataaagga aactttccca ttttcgaaaa cgatttgtcc taactctccg
    15181 tgctgagtcg attgaaaagc tagtagcgtt ttttcttgtt cttgcaaaga ttttaaaaag
    15241 ttctcatctg cactaccatg attgatatcc aaatatagat ttgttgactt tttcagctta
    15301 tctaatacag gaggaacaat ttgtggatgc aatctcacat tatcatacac agctaatttt
    15361 atcagcttat cacccatatc cgtccaagca gcaatatgaa ataccacatc tggtaaagct
    15421 gtgactaact cttctattcc ttctaaatct tgggaatttg tcaaagttaa acaagaaaat
    15481 tccttatcta aagacgatgt cagttcaaac tcccctatat gatgttgtaa aattttagtc
    15541 cattccaaat catgaaattc ccaccataaa tcacgataac gattggctat caaggtggtc
    15601 cagggttttc tgtaagacac aaaatgaata atactaggag gtactaactc attaaaataa
    15661 ttttcccatt tattataaaa agcagttaca tcatgaccca cttgaaggtt aaattcttta
    15721 tccagctcta accaatcatc ttgtaagacc tgattaaaaa tcgtttgatc accattaaaa
    15781 tgctcgaaac ggccttcttc aacttccttc attgtaacaa tgctctgttc aattagtcgt
    15841 tcttttaatt tctcttgacg ccatttttta ttgttgatta ataaaacacc tgcattaaat
    15901 gtaattccat ctgtatcttt aactgctgcg agtgattttt cttctaaaca aatactaaat
    15961 aatttctcta aagaagtatt tacaatcaaa tcactgtcta aatataaaac cttatcttct
    16021 tggatataat ctgcaataaa atatctagca taggtaatgc tactaatgtg atcttgcttt
    16081 tcccaatctt gaaacacagt ttgttcaggt agcttcacat cgataatctc actacctaac
    16141 atgcgagcta tttttcgtgg tttgcgaaac caatctggca taatatcttg atttaaaata
    16201 taaatcttaa catctcgatt gtggtataaa atagatttta tggttgtttc taactgcctg
    16261 atgtaattac gatcaccagc taatacaatt gttttaatca tcttgttctc ttattcaact
    16321 cccaaatggt aaattgcatc taccaataat tttttagtaa aataaccttc ttttagcaaa
    16381 taactaaagg tttttatccg attcgtcatt tcttgatatt cttgtagatt cattttatca
    16441 actatcgcat gaacttcttc tagactattc gccacaaagc ctaagccttg atctactata
    16501 aatttagcag ttgacaagct acttggtaca atgactggga tgcccgctgt cagataggta
    16561 ctcaccttat gagatatatt taaggtataa tattggttgc tttctccatc gttttgataa
    16621 gttccccaga caaggccaaa tccacccttt gataattcta gcaacaattc ctcatctttt
    16681 ttccatcctt cgatgctgag atttctagca ctagaactag cttccccttt atttgaaaat
    16741 actctcaaag gcgtatcttg agaccaattt tgtaagtctg gaaaacgctc taaacttcca
    16801 gcaaaaaaaa gttctttttt aaaagcaggg gtgtataagg ataaatcatg aggatgatcc
    16861 cacatccctt gaacaagaat cttcttagtc gtcaatcctt cttccatcag gcgtgttttc
    16921 attctctctg acggcactat caaaacatct gatagattat acatgtacat atagtctttc
    16981 atgagataat agttactatc aaacatgagg ggaacaacat catggataaa gcaaataatt
    17041 ttcacctgca tatccttcaa cttatcaaac aagagacgat caaattcaaa gccattccag
    17101 gttggagact gaaagactaa aatatcccca atagaaatac tggccataat accatccaga
    17161 cgcttattca tttcagaagg actatctgaa gcaatgttgt aaaaataaat accaacctct
    17221 ctaaatccca gttgactagc tatcttttga acagcatttt gagctaagat aaccgtacta
    17281 tcaccagcca tgccgtataa atttgttaaa tgtagtttca tattacgcta tctcattttt
    17341 tattactatc tacggaagta aagacaattt ttccatcatt cttctataaa tctctgtgaa
    17401 ctgcatattt tgttcttcta attttgctat cactctttgt aatctatttt tataaatcaa
    17461 aatatgattg gtcaagtcat agtctgaact tgctaaaata gctatctttt cttccctagc
    17521 cccgatttca tcatacatac gcttttcact ccatgtattc gataaagtat cagataaacc
    17581 aacacggtac cagtaaacac aacgattcaa atagactatc ctcgaagctc ttagaagtac
    17641 cttccatgtc cagtaagtat cctctgcaat ttttcctata ggaaatggta aatcttgtag
    17701 taactctctc ttgaataact tcaagacagc tacagtccag ttcccatttc tgacttcttc
    17761 gacaccttcc ctatccatga ttgctttacc ttctatcact tccagagaat catctggatc
    17821 cgtaacataa gtcatataca catagcgtgt ttcatcataa gaattataac gcccgatact
    17881 aatatctgcg ttttcctttt tcaaagcacc atataatcgg tctaaagcat catgttccaa
    17941 ccaatcatca gagtctacaa aagtaatgta cgcccccccc gaacattcaa tacctaggtt
    18001 acgagctgat gaaagaccgc cgtttgcttt ctcaaaatat ttgaaacgag aatctttctc
    18061 tacaaattct tcacatattt tggatgaatg atctggagag ccatcattaa ccatgataca
    18121 ttcaaaattc ggataagttt gtttttgaat actatccaaa cactgtcgca aataattctc
    18181 aacgttgtag attgggacta caatactaat tagttcattt tccatatact aactttccta
    18241 gacttgattt attctatttc cctcttaatc tcttctcttt acaacaagat ttccattatt
    18301 ataaactaca ctgtttgatg gaatatcatt gcgtatgaga caacctgctc caataatggt
    18361 attatcccca atcgtcactc cttttaaaat cgtgacatta cttcctatcc agcagtctct
    18421 gccaactcta atcggcgctg tagtccactg ccacttttca atcttttcag cagtataaat
    18481 atggtcgtga tcataaaaac gaacaccctc tcccatcatg gtaccagctc cgatttcaat
    18541 tctttccata cagttaaaac tacaagagtt gttgataaaa acaccatcat gcagtattaa
    18601 cttaccagaa gagacatgaa aattttcaaa atttctgcag atgacatttg ctcctacaat
    18661 gaagtgagcc ttatcatcga ttttaaagac attccaagtc ccatcttgga tgattttttc
    18721 ttgatgattg gtgcctactc tgacttcatt cagatagtct ctcactctag aaaccaattt
    18781 gtctggtgta cttgatagaa aaacttcctg cccctgattt ccatggactg tattgtcaaa
    18841 agcaaagaca gattttccag cctgtacaaa cttggagaca atagcatcta cttcttgaaa
    18901 gtgattgata tctagtaaaa agtcacaggt atcgactaga aagtttagct gttcttcatt
    18961 tgtaatcgtt cggagacgga cattatagtt cgtttctaag ctacggattt tctcggaaaa
    19021 ttgaacaggt gctgcaatat aaaaacagat atttggcaag gattcaagga gataatccaa
    19081 ctcttcaatc tgatcactaa cagtcaaaat cagagcatct cctactaaat gatttttctt
    19141 catttgctta tttctctcaa tttctcaatc attgcttgaa cttggtcaac agcatatacc
    19201 tcctgacctg cttcataggt tttggtattt tcaaaggaca aaatagtttt tccaagtctt
    19261 gcaaattgat tgataatttc ctctgttttt tcaccatgat taatatctaa aagtacttga
    19321 ctggtttcta ccaattcatt atcgacatct accaaatagt gaattccgtt aaatatagtc
    19381 acgtttggat aaattgtcat ctgagccaat cgatcactaa ctattactct agctgctatc
    19441 ttaaactgaa tatcaggcaa ggattgaacc aatgtctcaa tttgttcaat atggtctgag
    19501 gcagtataga ttagacaagt gaaaggttcc tttattggat agatgtgaga tctttgtaat
    19561 ggatgtaaat gatggttttg tcccaattct gtccattcaa gcccatgata gtaccaccaa
    19621 acttcacgat aggtttgggc cgccaaatct ttccacggtt tccgatgaga aagatagtga
    19681 ataatagcag gataatcctg accctcaggc aattgataat cagcaaactg tttatgaatg
    19741 acaatatgat tataatcaaa gtccaattcc aaccatttat gttcaaaaag catattcaag
    19801 atgctctgat ctgcctgatc caccttatca tgccattcat tggttacatc aattaatttt
    19861 tgggtcatat tctctttttt ccaaaaagca ttgtttacca agagaacacc ggcattaaag
    19921 atttcttgac caaaataagc tctgccccca aaatctctaa cagcagccaa aggataatct
    19981 tgtaagtctg tagcaaacaa atcatcaaga tttttcgtta caagtaaatc acagtccaag
    20041 tagagggcct tatcttcttg cacgaaatca gctatgaaat agcgtaaaaa aactgtgtaa
    20101 ctaatatccg ttttataaca tgaaatttgc tcagaagtta cccgacaatt aataatttct
    20161 gagtcaaact tctctaagcg cttatttaat tgcttaatcc attcatttgg aaaatcgcta
    20221 tgaatcagat aaaaacgaat cgaacgatta tgataacaaa tagacttgat tgtcgttaaa
    20281 acttggtcta catagccata gtttgctgct aggacaatgg ctttcttttc acttgattct
    20341 tgtaccgtta actgatttaa aagtcttttt ttcatttcaa actctttata tattgctgtg
    20401 tcagataaac cactagcttg accgttggcg agactgactt ccaacatctg acgataaact
    20461 gccaagtgtc tctctagagg atatcccata ttagctagta gcgtaatacg ttcagacata
    20521 gcatcaacta aggcgtgcat ccacttttct gtccaaactc ttgatagact accttttcta
    20581 atccgataag cataaagacc tttatttaaa taaattacct tttccgataa taaatatacc
    20641 ttttgattga ggtaaccatc ttctcctaat ttacccatgt caaagcgcaa ctgctcaaac
    20701 aatcttgcct tatagagttt accacaagca gatatcaaag caaaactctt catttcttga
    20761 gtttcataac aagttctcaa agatggaaac attgtcatat accttatcat aataggagtc
    20821 tcctgatata tgaaagtaga acattccttc actttcgttg aaagaatagt aattcccaac
    20881 tgcaatatca gcttgatatt ctgttatttt tttatatagg gtttctacat aatctaactc
    20941 aatccaatca tctgaatcca caaaggtcac ataatttccg gacatattat tcagaccggt
    21001 gtttcgtgcg gcagaaatac cagcattttc ttgttctata tagagaattc ggtgatccat
    21061 ttctgaaaat tctttacaaa tttcacctga agcatccgta gaaccatcat taacgacaac
    21121 aatctcaata tttttatatg tttgagaaat aatactatct agacatttcc tcagatagtt
    21181 ttctacattg tatactggta caatgaccgt aattttatca tccactagca attttctcct
    21241 tgatttatgt gcgcgagtta gtttagccta actttacagg ttgtcttact cttcttcatc
    21301 acgtttacgg cgtttcgcaa ccaatccaat acctgtaaca gctgctagaa ctccaagtaa
    21361 cacagatcca attgacgact cagtacctgt attaggcaat tctttttggg atttacctgt
    21421 cgatccagaa gcatttccaa cttgtgagtt cgaatggttt gctgaatttg aaactga
//
LOCUS       DAVBCG010000020        21218 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBCG010000020 DAVBCG010000000
VERSION     DAVBCG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.020"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQ495_000646"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQ495_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2530635.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQ495_000647"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQ495_000647"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2530636.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VQ495_000648"
     CDS             complement(4065..4628)
                     /locus_tag="VQ495_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2530637.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VQ495_000649"
     CDS             complement(4713..5267)
                     /locus_tag="VQ495_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2530638.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VQ495_000650"
     CDS             complement(5278..6501)
                     /locus_tag="VQ495_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2530639.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VQ495_000651"
     CDS             complement(6538..8988)
                     /locus_tag="VQ495_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2530640.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQAMGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9187..10116)
                     /locus_tag="VQ495_000652"
     CDS             complement(9187..10116)
                     /locus_tag="VQ495_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2530641.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10256..10543
                     /locus_tag="VQ495_000653"
     CDS             10256..10543
                     /locus_tag="VQ495_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2530642.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQ495_000654"
     CDS             complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQ495_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2530643.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQ495_000655"
     CDS             complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQ495_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2530644.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12684..15230)
                     /locus_tag="VQ495_000656"
     CDS             complement(12684..15230)
                     /locus_tag="VQ495_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2530645.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VQ495_000657"
     CDS             complement(15354..15794)
                     /locus_tag="VQ495_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2530646.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VQ495_000658"
     CDS             complement(15769..16638)
                     /locus_tag="VQ495_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2530647.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16680..17408)
                     /locus_tag="VQ495_000659"
     CDS             complement(16680..17408)
                     /locus_tag="VQ495_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530648.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17416..17895)
                     /locus_tag="VQ495_000660"
     CDS             complement(17416..17895)
                     /locus_tag="VQ495_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2530649.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17906..18604)
                     /locus_tag="VQ495_000661"
     CDS             complement(17906..18604)
                     /locus_tag="VQ495_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2530650.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18597..18863)
                     /locus_tag="VQ495_000662"
     CDS             complement(18597..18863)
                     /locus_tag="VQ495_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2530651.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18929..19855)
                     /locus_tag="VQ495_000663"
     CDS             complement(18929..19855)
                     /locus_tag="VQ495_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2530652.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19930..20745)
                     /locus_tag="VQ495_000664"
     CDS             complement(19930..20745)
                     /locus_tag="VQ495_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530653.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20749..21069)
                     /locus_tag="VQ495_000665"
     CDS             complement(20749..21069)
                     /locus_tag="VQ495_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2530654.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6415 a         4659 c         3715 g         6429 t
ORIGIN      
        1 aagttacttc aaagtacaga tttgaagctg cagataagac tgacgaaatc actttaatac
       61 taaatgaaaa tcaaaaagca aactaagaag ctagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaagtgg
      181 tggataaatc ggctgggtaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat aaccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gcgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattgataac acgagtcagt gccgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtgatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat cagtgataga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaaggtt gttgtttcag taaagatttt tgggtccggt atgaagtgaa ccgttgttcc
     3541 agttctatct gtatccccaa ccacctcaag gtctgcaaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccgt ttttatggac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcatgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttaaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatctcga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaacata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttgatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcgaaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tgaaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactat aatcgactct
     6481 agagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagttcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgatgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca caaaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccata accacttctt gttcctgttt tccccgagat tcaatcttga caaatttggc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccatg gcttggcaac tctcaggaag taaagaggac agaagagtac ggcctgattt
     7321 tgtagaagaa atctgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagccttct acttctaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttaccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggtcacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 atagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gctgccgttt tcttaaaata
     8341 cagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctgag tcatcttttc ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt tttaaaatag
     9121 tgaagaaata cgaaaaattg agagaagaaa tgttttcatc ttccttctct caacgaatca
     9181 tgtattttat ttaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9241 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttggtcat ggctaaagtt
     9301 gccctcttga atcaagctac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9361 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9421 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9481 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
     9541 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
     9601 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
     9661 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
     9721 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
     9781 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
     9841 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
     9901 atcttggtgc atgagagcat tgaccgcaac gagcaattcc ttaccagcat catgaaccaa
     9961 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10021 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10081 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10141 caaaatcatc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10201 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10261 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10321 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10381 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10441 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10501 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    10561 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    10621 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    10681 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccga gttctagcct
    10741 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    10801 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    10861 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    10921 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    10981 tattttccga agcaatcatc tcgtagtttg tgaaagttgt attaaaaaag ctagttggaa
    11041 cttctgcaag ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11101 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca
    11161 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11221 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11281 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11341 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgt tccaactgac
    11401 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11461 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    11521 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    11581 cagaaagaag ttgagtaaag tctttatcaa agagaatgac ttccgtattg gaactgacat
    11641 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    11701 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    11761 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    11821 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    11881 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaaa
    11941 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12001 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12061 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12121 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12181 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12241 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12301 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12361 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12421 aaatccagca aaatcaagtc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12481 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    12541 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    12601 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    12661 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    12721 gcagcttttt cacggctaat caagtcaaca cgcgctgcaa tttccttgat tcccataccg
    12781 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    12841 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    12901 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    12961 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13021 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13081 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13141 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13201 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13261 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13321 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13381 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13441 tcaataaagc gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13501 tcttccttag caagtttatc aagaactgga agcaagtctg cataagcaat gtgccctgcc
    13561 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    13621 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    13681 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg
    13741 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    13801 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    13861 ttcaagacat cattttcaac tttaacagta agaactgggt aaccaggctg ttccaaccaa
    13921 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    13981 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg cgtgcaaacc tttagcaaaa
    14041 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14101 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14161 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14221 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagcg
    14281 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14341 tggtgagcca attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14401 ttctcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14461 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    14521 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    14581 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    14641 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    14701 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    14761 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    14821 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    14881 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    14941 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15001 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15061 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15121 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15181 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15241 tattctaagt ttaagagcta ctgctcttct tcaaacaaac ttattatatc atattttgct
    15301 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcagccct
    15421 acttcaccca aacagtctgt gaaaaagtcc tcatgaacct ggcgccaata ggcaagtgat
    15481 ttgtctccct caccttcctt aaaggcatgt tgcgcagaaa cttgattgaa gagttcaaca
    15541 gaaacctttg taatttcgac aatgcagaca gactgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagtacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaagcat cgatttcatc tccgatcaaa ggattgattt gcttgtattt attccacatt
    15781 tcttgcggta tcatgggtac tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatta atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccagatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaagc catactgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa agataacaga
    16261 tagattgggg ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tggttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactcttta gtccccacta aacaattggc ttctgtattc gttaattcag taaaacgata
    16441 ccaaacttct tcatctgagt acagggagca gatagtctct agataatctt ttacaaattg
    16501 ctgacaactt gctaatatga tgttttgatt aagctctctc aacaaatatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatag ccattcacct cgtcttaaaa gatacaatat catatataat
    16681 tatatcaaaa tttcctctag ttcaaaagct tcataaggtt cagataactc atatcctaaa
    16741 aattctgcta cttttttgga agcctcatta tgagcatccc aaagtggaaa catatctcta
    16801 tttaaactct ctagaatcat tgcagcacca agctttttag caaatccatt tccttgttcg
    16861 tttggtctag ttgcaacttc cacttcaact gctttacggt aaactaaccc tgaggaaatc
    16921 ccagcaatca gttcattatt tttaagaatc acaaagccaa atccaccttt taaaacaaaa
    16981 tcctgatagg actcaaaatc cccctgtaaa tcctgtgacc attcttccgt agaaaagcag
    17041 ttataaatat gattatcaat aggcacaata ttgtaacctt cctctaaatg agtaactaga
    17101 ttatttaaaa attcaacttg aaaatttgcc ttatctttaa aagaatagcg agtaaaagaa
    17161 tttagcccca cttttcgatg cagaaaatct tgccaattag tattttctga aataataatc
    17221 ttataatcta aaccatattt tctaacaaaa tcttcccaaa atctggaatc caactcccct
    17281 gctaggtata gaaaattccc aacatcataa aagcttgttg tcctatcatt gtttcggtag
    17341 acagttccaa tttgagattt aagaccatag agaaccatat ttttcttcca tgttttaaat
    17401 agttccatta tccctctact tcaccttcca attcatgtaa gaagtcttgt ccagttctgg
    17461 aaatcctaat aattcagact taaccttcaa gactaatggc gatgcatttt cttctgtaat
    17521 ctcttgaata tccatccaaa tatatccaag tgaatcattc gcaccatcag acacagcttc
    17581 cgaagtcgta acttgaggtg cactctcatt catttcaaca tcatacaagg ccatgacatg
    17641 gtcaaccata aaatttgtta actcttctct gacgaaaaca tcgtagattc gaggattaga
    17701 gtagcttcta acagtaaatc ccgtctcttc cataacttct ctagtcagcg tttccgtcag
    17761 tccttcacca agttgctgac tgcctccagg tagatcatac cgatgttgat aagggcctct
    17821 cgttttttca atgcagagta acttcccatt ttctaagcaa acagcgtaga ctccaaagtg
    17881 ttttttgatt tccatccaac tcctcctact tcaaagacca gccaccatct attgtcaaga
    17941 tttgtccttg catggcgctc gcttttccac ttgctaaaaa aagactaagc tctgctattt
    18001 cctctggctc aatccagcgc ttgattggag tttcactagc cacccagtca gccaaaccac
    18061 ctggttcaaa atccgcagcg gtcatagctg tcttgactgc tcctggagcg ataccaaaga
    18121 cctgaatccc agcttcagca tagtctagag ccaactgctt ggtgaagcca gccaaggcat
    18181 gcttggatga agtataggcg tgaccacctc cacctgctag gctagaagca atggaacaca
    18241 tattgatgat gattcccttt ttattttcca gcatttgtgt caaataatac cgagtcaact
    18301 ctactggaat aatgtagttg atttcaaaaa tctcttgaat gtcctgcgcc gtttgttcca
    18361 acagtggttt gtaatcatcc aaaactccag cagtattaca caaaacatcc acctgagggc
    18421 accagtcaaa aataggttcc aagtccaagg tcaaatctct ctgtaaaaag tgaaaatcac
    18481 cctctaagag tggcttttca ccttggtcaa ctccataaac ttgatagccc ttctctaaaa
    18541 agaggcgagc ttgagccaat ccgatccctg aactcactcc tgtaatgagt acacgtttag
    18601 tcatgcactt ctacccaatc cgttgccaaa acatcacaaa ctgtcgggct ccacatggaa
    18661 aaaccttctc cttcgccaga aacgttgatt aggaaataag gtgtcatttc aagtgcaagc
    18721 ccattttgct cgatggtatc aaagagttgg acatagtttt ccgcacctcc ccaaccagtt
    18781 cgtacatatt ttctcttagc ctttaaccca ggcaggatct cttcaaatgt catgtttttc
    18841 tcctttaatt ctacattctt catttaatta tagcaaaaaa ccgctttata cggctttttg
    18901 aatgtgagtt attcaaacct gctactactt acggcaaatt attccctgca gcaagataaa
    18961 tttcatacca ttcttttctt gttaagctaa agtttgccgc tcggctaact tctctcaagt
    19021 gcttaggatt tgttgtacct acgactgcct gcatttttgc tggataacgc aatatccaag
    19081 aaatggcaat agttgaagag gttactccat atttaatagc taaacgatca agtacttgat
    19141 ttaaagcttg aaatttctca tttccaacaa aattcccttt aaaatacccg aattgtaaga
    19201 cagaccatgc ttgaatgacc acatcgtgta attggcaata ttcaaaaatg ctgccatctc
    19261 gcatagctgc ttgactatct tccatattaa catgaaaagc tgattcaaat cctggagtaa
    19321 aagccgcact caattgtagc tgattaacag ctaacggctg cttgacatct tttttaagca
    19381 actccatcat cataggattt tgattagaaa ctccaaaatc tcgaacttta ccttgtttat
    19441 aaaggagatt aaaggcttct gctacttggt cagattccat caaagcatct ggtcgatgaa
    19501 ggagcaagct atctagatga tcaatcttca atctttgcaa aataccgtct actgatttta
    19561 taatatagtc cttagaaaaa tcaaaatagg taaattcttc aatgcgaatg ccacatttgg
    19621 actgaatcca catcttttct cttaaatctg gacgattttt taggacaaga cctaacagtt
    19681 cttcacaacg accacgacca taaatatcag ccaagtcgaa ggcattgatt ccaacagaaa
    19741 gtgctgtttc tacaagctct tcaacttctt ttacagactt atcttttatt ctcatcattc
    19801 cgagaacaat ttctgataat tctttgtcat cttgaccaag agttatgtat ctcatcaaat
    19861 ttttctcctt taatttctaa cattcttccc ttcattataa caaaaaaccg ctttgcaacg
    19921 gctttttgac tatacttcac tccattttat cttcttaaac ccacggaaca agacaaagat
    19981 tccaataaag aggacagcta aaggaataac ttttgtaagg aaaacatttg aaattcccat
    20041 ccactcataa taacggagca gagaacccac cacaagatgg gcaataatca tactgacaaa
    20101 tggacgaaag accgcttctt tccaattcca aataccgata actagcgaaa tcgtaaagac
    20161 agacaaacta tcccagggag ccggaatata aaaggctcct tcttgtatga agcttgccat
    20221 tcctacatat cctaaaacaa ctagaagaac tatagtccca acaacaatgt aagtgccaat
    20281 tttcatttta ggagaatctt ggactaaact tcttcgtaaa attgtggcca caagtccaaa
    20341 tccaatcaga aaaataagaa gttgccctaa aaatgtgagc aaattgactg ttaagagagg
    20401 acctttagaa aaatcactta gtagttgata ataacgtaat acagccagga caagaattgg
    20461 cgtcaaaagg gactctttga tagaactgcg aggtgctccc ttgagaatct ctttcattat
    20521 ttttttagga ttcttaccta gataatcctc tgcactcatg ccatctcgtt ctgcttctga
    20581 gaaatctagc atcatcaaat agatctgctc tctgagatag tcttcatcat agagaaatcc
    20641 agcaagatta aaactttccc acaactcctc aaaatacttt tgattctcct cagaaaactc
    20701 atgtagcaaa gcgcttgttt cttcgtaata cttcattttc ttcatggttt aacccccatt
    20761 cttaatccct tctacttttt gactcaaatc gtcccattgc tgccaaaaga ctgagacacg
    20821 ctcttctcct tctttcgtta atgaaaaata cttccgatct ggaccatctg gcgacgggcg
    20881 catgtcgcct cttatccatt gatttttttc taacttttgc aacaaaggat aaatagttcc
    20941 tggaacgata gtatcaaatc cagcctctcg caaagtctga accaactcat aaccataccg
    21001 ctctttttga ccaatcatat ccaagacaca accttcaaga acacctttta atagctgagt
    21061 ttctttcatc acttctccct tctaatctat tttgtaatac ctactagtaa cttcacctat
    21121 agtatattac ttctacacta gtttgtaaag cataatagtt aatactcttc gaaaatctct
    21181 tcaaaccacg tcagcgtcgc cctaccgtat gtatggtt
//
LOCUS       DAVBCG010000021        20623 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBCG010000021 DAVBCG010000000
VERSION     DAVBCG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.021"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..176
                     /locus_tag="VQ495_000666"
     CDS             <1..176
                     /locus_tag="VQ495_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET2530655.1"
                     /translation="FFGILKSEMFYGYEKSFRSLENLEQAIVDYIDYYNNKRIKVKLK
                     ELSPVQYRTKSFG"
     gene            complement(224..2767)
                     /locus_tag="VQ495_000667"
     CDS             complement(224..2767)
                     /locus_tag="VQ495_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2530656.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2829..4451)
                     /locus_tag="VQ495_000668"
     CDS             complement(2829..4451)
                     /locus_tag="VQ495_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2530657.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4657..5682
                     /gene="trpS"
                     /locus_tag="VQ495_000669"
     CDS             4657..5682
                     /gene="trpS"
                     /locus_tag="VQ495_000669"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2530658.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5835..7313
                     /gene="guaB"
                     /locus_tag="VQ495_000670"
     CDS             5835..7313
                     /gene="guaB"
                     /locus_tag="VQ495_000670"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2530659.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7364..8461)
                     /gene="recF"
                     /locus_tag="VQ495_000671"
     CDS             complement(7364..8461)
                     /gene="recF"
                     /locus_tag="VQ495_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2530660.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8464..8832)
                     /locus_tag="VQ495_000672"
     CDS             complement(8464..8832)
                     /locus_tag="VQ495_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2530661.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8991..10241
                     /locus_tag="VQ495_000673"
     CDS             8991..10241
                     /locus_tag="VQ495_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2530662.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLFDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10238..11521
                     /locus_tag="VQ495_000674"
     CDS             10238..11521
                     /locus_tag="VQ495_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2530663.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11563..12384
                     /locus_tag="VQ495_000675"
     CDS             11563..12384
                     /locus_tag="VQ495_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2530664.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12395..12940
                     /gene="pgsA"
                     /locus_tag="VQ495_000676"
     CDS             12395..12940
                     /gene="pgsA"
                     /locus_tag="VQ495_000676"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2530665.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIILIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            12937..13764
                     /locus_tag="VQ495_000677"
     CDS             12937..13764
                     /locus_tag="VQ495_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2530666.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13749..14588
                     /locus_tag="VQ495_000678"
     CDS             13749..14588
                     /locus_tag="VQ495_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2530667.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14581..15375
                     /locus_tag="VQ495_000679"
     CDS             14581..15375
                     /locus_tag="VQ495_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2530668.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15434..16252
                     /gene="mreC"
                     /locus_tag="VQ495_000680"
     CDS             15434..16252
                     /gene="mreC"
                     /locus_tag="VQ495_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2530669.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTVVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16252..16746
                     /gene="mreD"
                     /locus_tag="VQ495_000681"
     CDS             16252..16746
                     /gene="mreD"
                     /locus_tag="VQ495_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2530670.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16840..18018
                     /locus_tag="VQ495_000682"
     CDS             16840..18018
                     /locus_tag="VQ495_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2530671.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18242..19021
                     /gene="rpsB"
                     /locus_tag="VQ495_000683"
     CDS             18242..19021
                     /gene="rpsB"
                     /locus_tag="VQ495_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2530672.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            19099..20139
                     /locus_tag="VQ495_000684"
     CDS             19099..20139
                     /locus_tag="VQ495_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2530673.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            20385..20623
                     /gene="tnpB"
                     /locus_tag="VQ495_000685"
                     /pseudo
     CDS             20385..20623
                     /gene="tnpB"
                     /locus_tag="VQ495_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         6560 a         3293 c         4269 g         6501 t
ORIGIN      
        1 ctttctttgg gattctgaaa tcggaaatgt tttacggtta tgagaagagc tttagatctt
       61 tagaaaacct tgaacaagct attgtggact acattgatta ctacaacaac aaacgaatta
      121 aggtaaaact aaaagaactt agccctgtgc aatacagaac taaatccttc ggataaatta
      181 attgtctaac tttttggggt cagtacatta actggtcgat tttttatgcc ttagatgact
      241 ttcgtctgtg attatagatt ccaaatagta agagagaagt aaaggaacag attgctccag
      301 taataaaacc attgggaatg aaggaaagtg taatagttcc tttccccttg ggaatgtcaa
      361 ctttcataaa tccagtttga gcttgtttaa tttctatttt cttaccatct tggtaggcag
      421 accaaccttt gtcataagga atggtgaaga aaatagatgt atcttgttgg acatcatatg
      481 tagcaaaaac cttgttttta gaagttgata ctgtgacagg ttgttcttta attttttgaa
      541 ttgcctcggt gaaagttttg gtatctaaac gatagaaggt aggagattca aatgatactt
      601 gtgaatttcc agggaaacta acattgatat tgaaagtttt tttctcttta gtatatccta
      661 gattaaagaa ggagaagaca ttatcagttg taaaagtctt tttttcacca tttacaagga
      721 tgtcaacctt cttttgttta tcgttagaaa agtgaaggtt tatgaaagag agataaactt
      781 ggctgttttc tggaacttca atttgatact ggattgctgc atcttcattt gaagaacttg
      841 tgacactaat caaatcatta gtattttctg ttttttcata aggtattgga gaaaaataat
      901 caaaattgac gttagcaagt tgatttaaaa atgaggcctg attatccaag gtatgttcat
      961 tgaacttgac atcattgtaa acagattgac tagcaactgc aatcggaaga gagtattgat
     1021 tttcatatag ggtaagatta tctttttgat agatatcttt aaagccatac ttatcaatag
     1081 gactgtctga gatattgtac tggataccaa ataaactatc agccaaaata ctattatttg
     1141 catatcggag attgagatta gtcccagagg atttaaaacc aagtttatct aaagtagagc
     1201 ttgatgaacg atttcgaaca gatgaaaatt gagagattcc attgtagttg aatttcatac
     1261 tgtcatttcc tgtctgagtt tgtagttttt cagtacgagt aaattgattt ccaatatatg
     1321 ttgagaaaga ttccatagct gggatatctc gactataagc acttcgagaa gcaaatcccc
     1381 attccttagc aattccgtcc atttgagatg aagcatttaa actcatttca accagtataa
     1441 ataaagagat tagaatggca aatagattca cagatataaa ctttttgata actgcaagga
     1501 gtaaaagaga atagacaacc aaaaattcaa gagtaagcag aatattcaaa tctgttaaaa
     1561 aagaataatg cgattttaga tagatggtag ctaaaaatcc tgctactaca agaaaaagcg
     1621 aaactaaaaa attccagact ttaagttctt tcagacgctt taagacttct gctgctgtgt
     1681 aaattaacaa ggtagagaaa atccaagcat agcgatgtaa aaacatgttt ggagtatgca
     1741 tgccttgcca aaataagtca agagcttcta tgtaaaagct tgcaattaga aatgcaaaga
     1801 atattacata tatgagtttc acgtgaaact taatagattt cagcgtaaaa aataaaatgg
     1861 tcaaaataaa gggaaatagt ccaacaaaaa tcattgggat ggccccatac tttgttgtgt
     1921 caaaggaacc aatgaattgc ttagcaaaga gatcaagata ccagctactt tcagtttgaa
     1981 actttgtaac ttcagtcaat ttttccccat gtgtctgtaa atcaaataga gtgggaagag
     2041 tcataatcaa actagccata ccagctaaaa aggagataac tatgaaatca agaacagatg
     2101 attttcgagt cttaaagtcc cacgaaattt gacagagata ccagaaaata agaaacaata
     2161 ctgtcatata tccaaaataa taattttgaa taaataagat tgacagactt gtaaagtaca
     2221 ataggagttt cttttcagtt atcagtagat gtaaaccagt tataattaaa ggaatcaaga
     2281 taaaaacatc tagccaggtt tttatctcta attgactgac agtgaaactc atcagagcat
     2341 aggaagtaga taaggctagt tttaaagtct gagggataga ttgaaacaat ttattcaaac
     2401 taaaaaaggt tgacagacca atcaatccaa attttaagag agtggtcaga tagacagcat
     2461 ctggcatatt cgttagatca aaaaagtaaa ctagaggcgc gagaaaacta cccaagtaat
     2521 aactagatag ggcatagaag tttagcccta gaccacttgt aaaggtgtaa aacagactac
     2581 tatttccatg taggatattt cgtaaggcta catcaaaaat aacgtattga tgaaagccat
     2641 ctcctaatag aggagagttg tcgctattcc agtagatacc ttgagataga tatactccag
     2701 acataatcac tacaggaatg atgaaagaaa taaaataggt tcgatatgtt tttaaaaatg
     2761 atttcatgtt acctcgtaga atgatagaaa actcagttgg ttaacccaac tgagttttga
     2821 agttttattt agtctttcca aagttcttta acttttgctt gtacttctgc attttctagg
     2881 aattcatcgt aggtttcatc gatacggtca atgacgccat ttttagataa gacaatgata
     2941 tggttagcca aagtttgaat aaactcgtgg tcatggctgg caaagatgat tgattcttta
     3001 aagtttttca gtccatcatt caagcttgag atagattcca agtccaagtg atttgttgga
     3061 tcatcaagta caaggacatt tgattttaag agcatgagtt ttgaaagcat gacacgaact
     3121 ttttctcccc ctgacaagac atttacaggt ttgttaactt catctccaga gaagagcata
     3181 cggccgagga aaccacgtag gaaagtattg tcatcttctt ctttacttgc gaattgacgc
     3241 aaccagtcaa gaattgattc tcctcctgca aaatcagctg agttatcttt tggtaggtaa
     3301 gattgactag ttgtaactcc ccacttgaca gttccttcat agtcaatatc tcccatgatt
     3361 gcacgaatta atgcagtcgt ttgaatatca ttttgtccaa taagtgctgt cttatcatct
     3421 ggacgcaaga tgaaactaat attatccaag atagtttcac catcaatctt tacagttaga
     3481 ttttctactg tcaagagatc attaccaatc tcacgttccg ctttaaagtt gataaatgga
     3541 tatttacgac tagatggtac aatctcttct agctcaatct tatcaagcat tttcttacgt
     3601 gatgttgcct gccttgactt agaagcattg gcagagaaac gagcaacgaa ttcttgcaat
     3661 tgtttaattt tttcttctgc tttagcatta cggtctgcta gcaatttagc agcaagctca
     3721 gaagattcct tccagaagtc gtagtttccg acatagagtt tgatttttcc aaagtcaagg
     3781 tcggccatgt gagtacaaac tttgtttaag aagtgacggt cgtgggatac tacgataact
     3841 gtgttatcaa agtcaatcaa gaagtcttct aaccaagtaa tcgattggat atccaaaccg
     3901 ttagtaggct cgtccaagag aagaacatct ggtttaccaa aaagtgcttt ggcgaggaga
     3961 acctttactt tttcaccgtt ggccaattcg ctcatgtttt ggtagtgtaa ttcttctgga
     4021 atgtttaggt tttgaagtag ttgagaggct tcactctctg cttcccaacc tccaagttcg
     4081 gcaaactctc cttcgagttc ggcagcacga accccgtcct cgtctgagaa atcttccttc
     4141 atgtagatag catctttctc tttcatgatg ctataaagtt tttcatttcc catgataacg
     4201 acatcaatgg cacgttcatc ttcgtagtca aagtgatttt gacgaagaac agagagacgt
     4261 tcatctggac caagagagat gtgaccagta gtaggttcga tatctccagc taaaattttt
     4321 aaaaaggttg attttccggc accattagca ccgattaatc cgtaagtatt tccttctgta
     4381 aatttgatat tgacatcatc aaaaagtttg cgatcactaa aacgtagtga aacatcagat
     4441 actgtaagca atgtttttct cctatatgtg taatatattt attctactag aaaatacaga
     4501 aatattcaaa tttttatttg tcaattttgt gtaaattata tttacagtat cctttacaca
     4561 aatctgtaaa aagcaaggct gatttatttt gataaattac ggttatttca ttaaaaaaat
     4621 gctataattg aaaggactat atcgaaggag aacaaaatga ctaaacccat tattttaaca
     4681 ggagaccgtc caacaggaaa attgcatatt ggacattatg ttggaagtct caaaaatcga
     4741 gtattattac aggaagagga taagtatgat atgtttgtgt tcttggctga ccaacaagcc
     4801 ttgacagatc atgccaaaga tcctcaaacc attgtagagt ctatcggaaa tgtggctttg
     4861 gattatcttg cagttggatt ggatccaaat aagtcaacta tttttattca aagccagatt
     4921 ccagagttgg ctgagttgtc tatgtattat atgaatctag tttcgttagc acgtttggag
     4981 cgaaatccaa cagtcaagac agagatttct cagaaaggat ttggagaaag cattccgaca
     5041 ggattcttgg tctatccaat cgctcaagca gctgatatca cagctttcaa ggctaattat
     5101 gttcctgttg ggacagatca gaaaccaatg attgagcaaa ctcgtgaaat tgttcgttct
     5161 tttaacaatg catataactg tgatgtcttg gtagagccgg aaggtattta tccagaaaat
     5221 gagagagcag ggcgtttgcc tggtttagat ggaaatgcta aaatgtctaa atcactaaat
     5281 aatggtattt atttagctga tgatgcggat actttgcgta aaaaagtaat gagtatgtat
     5341 acagatccag atcatatccg cgttgaggat ccaggtaaga ttgagggaaa tatggttttc
     5401 cattatctag atgtttttgg tcgtccagaa gatgctcaag aaattgctga tatgaaagaa
     5461 cgttatcaac gaggtggtct tggtgatgtg aagaccaagc gttatctact tgaaatatta
     5521 gaacgtgaac tgggtcctat tcgtgagcgc cgtattgaat ttgctaagga tatgggagaa
     5581 gtttataata tgattcaaaa aggtagtgaa agagcgcgtg aagttgcggg tcaaacccta
     5641 tctgaggtaa aaggggcaat gggacttcat tactttaact aagtttattt tacaagaaac
     5701 tatcatttct atctcgaaga aaagatagtt tttttattta cccctgtaac atttgacaaa
     5761 tttttataga atggtatgat agatacaata taaaaagagt caagctcaaa aagaaagaaa
     5821 agaggaaact tcgaatgtct aattgggaca ctaaattttt gaaaaaaggt tttacctttg
     5881 atgatgtatt gcttattcca gctgaaagtc atgtgttgcc taacgatgca gatttaacaa
     5941 ctaaattggc agataatttg actttaaata tcccaattat taccgctgcc atggacacag
     6001 ttacagagag tcaaatggcc attgctattg ctcgtgcagg cggtctcgga gttatccata
     6061 aaaacatgtc aattgctcaa caagcagacg aggttcgtaa ggtaaaacgt tctgaaaatg
     6121 gagttattat tgatccgttc ttcttgacgc ctgaacatac aattgctgaa gcagatgagc
     6181 ttatgggtcg ttaccgcatc agtggtgttc cagttgttga aacacttgaa aatcgtaaat
     6241 tggttggtat tttgacaaac cgagatcttc gttttatttc agattataat caaccaattt
     6301 caaaccatat gactagtgaa aatcttgtta ctgctcctgt gggtacggat cttgcaacgg
     6361 ctgagagtat tcttcaagag catcgtattg aaaaacttcc gttggtcgat gaagaaggca
     6421 gtctttctgg tttgatcact atcaaagata ttgaaaaagt tattgagttt ccaaatgcgg
     6481 ctaaagatga gtttggtcgt cttctagttg caggtgcagt aggtgttact tcagatacat
     6541 ttgaacgtgc agaggctctt tttgaggcag gagcggatgc gattgttatt gatactgcac
     6601 atggtcattc tgcaggtgtc ttgcgtaaaa ttgccgagat tcgtgctcat ttcccagatc
     6661 ggactttgat tgctggaaat attgctactg ctgaaggtgc acgtgccctt tatgaagcgg
     6721 gtgtagacgt tgttaaggtt ggtattggac caggttctat ctgtactact cgtgtgattg
     6781 ctggtgttgg tgttccgcaa gtaacagcta tctacgatgc tgcagctgtt gcgcgcgaat
     6841 atggtaaaac gattattgct gacggtggga tcaagtattc tggagatatt gtaaaagcac
     6901 ttgctgcagg tggaaatgct gttatgcttg gatctatgtt tgctggaact gatgaagctc
     6961 caggcgaaac tgaaatcttc caaggacgta aattcaagac ttaccgtggt atgggatcaa
     7021 ttgctgctat gaagaaaggt tcaagcgacc gttatttcca aggttctgtc aatgaagcaa
     7081 acaagcttgt tccagaagga attgaaggtc gtgttgctta taaaggagcg gcagctgata
     7141 ttgttttcca aatgattggt ggtattcgct ctggtatggg ttactgtggt gcagctaacc
     7201 ttaaagaact acacgataat gctcaattta ttgaaatgtc tggtgctggt ttgaaagaaa
     7261 gccatcctca tgatgtgcaa attactaatg aggcaccaaa ttattctatg taaaaaacaa
     7321 tgaaaagaac tccagtgaaa acaggagttc ttttacaatg ttgtcaattt ccatttacag
     7381 cagctttacc atcctgaata gtgaagatac ttagattttc tggcagattt tgaagatggt
     7441 ctaagcttgt tgttgtgata aaggtttgga ttgattgaga aatcgtttct aataatttta
     7501 actgtctagt gttgtcaagt tcactcatca catcgtcaag cagtaatata ggagattctg
     7561 tggtaatgct ttccattaat tcgatttctg ctaattttat cgagaggacg agactacgat
     7621 gttgaccttg gcttccgaaa ctagcatcca tcccatttat ataaaaagaa atgtcatctc
     7681 gatgaggacc gacaccagta ttctttttaa ataaatctct ggatctactt ttttctaaag
     7741 caattttgaa agattcggat aagttttgtt tgtcagttat attgacagaa gattgatagg
     7801 atattgacaa ctcttcgatc tgattagaga gttcaaaatg tttcttacgc ccaaatgatt
     7861 ctagtttttt tatgaaatct aagcggtgat tcattacacg acatccataa tcaactagct
     7921 gatcatctaa cacagaaagg aatgtttcat ctattttttg agctgatttt aggtaagtgt
     7981 ttctttgctt taggatgtgg ttataattgg ttaagtcaga taaatagatt ggcttaattt
     8041 gcccaagttc catatcaatg aattttcgtc gaatcgaagg tgctccttta attagttgta
     8101 aatcttcagg agcaaataag acaacattca tgtgtcctac ataatctgaa aggcgtgcct
     8161 gttttaagtg attaactttt gttacacggc ctttttgtgt tagttcgatt tctaggggaa
     8221 tggatccagt ttttttctga acgagacctg aaagatgaag ttgttcctca tcaaaatgaa
     8281 tgagattttt atctgttcga gttcgatgac tacgcgttaa ggctaaaaaa tagatagcct
     8341 ctaacatgtt tgtttttcct tgtgcattac gtcccaaaaa gacattcaat ttaggattaa
     8401 agtctatttt cgtttctttg taattacgaa aagtcttgag agatagatgt tgtagccaca
     8461 tgattatcta ccagggaatc gtggagcttg ttggctttta ggtgatgaag caggtttcga
     8521 tttgtctttt ttaactccct tattcatctt tttgacaagt ttagcgatcc gttctttttc
     8581 aactttatca gcttggtatt catcttgttc ttcagaagta ggttgtgtca acaagatgtc
     8641 aatgttcatg tcagggatgt caactttatc gccaatacga agtttttttc cacgacaact
     8701 ctctaattcc ctattaaagt aaacagaatg ttcagagaga aatgatttaa tagctcctcc
     8761 gctatgtgta attccaagtt ctttgagtag tgcttggagg gtaataaatt cttcaaataa
     8821 tttgtattcc ataattcacc tcaatctttg gtattatacc atattttcct aaaaatagtg
     8881 agagagacag ctagaaatgt aagcgatttt tatttcaaaa gtgttacagg gaacaagaaa
     8941 atttcaggtt ttcgtgatat aatagaagtc tgtatataag gaggtaaatc atggagttag
     9001 tgcatggaat ttcaacacat tttatccaat caaaaaagtt taaaacaaac aaaattaccg
     9061 tgcgttttac cgctccatta tcccttgata cgattgcagg tcacatgttg agtgcaagta
     9121 tgctagagac tgctaatcag atgtacccca cttctcaaga tttgaggaga cacttggcca
     9181 gtctatacgg tacagatatg tcaaccaatt gtttcagaag agggcaaagc cacattatag
     9241 aattgacatt tacctatgtt cgtgatgagt ttttaagtag gaaaaacgtg ctaacctctc
     9301 agattttgga acttgtaaaa gaaactcttt tttcacccgc agtagttgat aatgggtttg
     9361 atccggcctt atttgaaatt gagaaaaaac aattgctagc aagtttagca gctgatatgg
     9421 atgattcttt ttattttgca cataaagaat tggataaatt gttttttcat gatgaacgtc
     9481 ttcaattgga atatagtgat ttacgaaatc gtattttagc tgaaactcca caaagttctt
     9541 attcttgttt ccaagaattt ttagccaatg atcgaataga tttctttttc ctaggtgatt
     9601 ttaatgaggt tgaaattcaa aatgtattag aatcatttgg ctttaaaggt cgaaaaggag
     9661 atgtgaaggt tcagtattgt caaccttatt ctaatatcct tcaggaaggt atggttcgga
     9721 aaaatgtggg acaatccatt ttggaattag gttatcatta ccgttctaaa tatggtgatg
     9781 aacaacattt acccatgatt gtaatgaatg gtttacttgg tggatttgct cactctaagc
     9841 tctttacaaa tgtccgtgaa aatgctggat tagcttatac catttcaagt gagcttgatt
     9901 tatttagtgg attcttgagg atgtatgctg gtatcaatcg agaaaatcgt aaccaggctc
     9961 gtaaaatgat gaataatcaa ctgtttgatt taaaaaaagg ttattttaca gagtttgagt
    10021 taaatcagac caaggaaatg attcgttggt cgttgttact ttctcaagat aatcaatctt
    10081 cattgattga acgtgcttat caaaatgcct tatttggaaa atcttcagca gactttaaag
    10141 gttggattgc aaaactcgag caagttgaca aagatgctat ttgtagagca gctaataatg
    10201 tgaaactaca ggcgatttac tttatggaag gaatagaatg acaaaggttg tttttgaaaa
    10261 aaaatactat ccagctgtaa aagaaatgat ttatcgaact cgtttggcca acggattgac
    10321 agttgctctt ttgcctaaaa aggaatttaa agaggtttac gggagtgtca ctgtacagtt
    10381 tggttcggta gatacgtttg tcacagaagt tgacggagat gtaaaacaat atcctggagg
    10441 aattgctcat tttcttgaac ataaattatt tgagagagaa gattctagtg atttgatgtc
    10501 ggcttttacg agtctaggtg cagatagtaa tgcctttaca agctttacaa aaacaaacta
    10561 tcttttttca gcaacggatt attttttaga aaatttagat ttacttgatg aattggtaac
    10621 atcagcacac tttactgaag cttccattct gacagagcag gatattattc agcaagaacg
    10681 agaaatgtac caagatgatc cagattcgtg tttattcttt tcaactttag cgaatttgta
    10741 tcctggtaca cctttagcaa ctgatatagt tggaagtgag gagtccattt cccaaataaa
    10801 tctaactaat ttgcaagaaa attttacaaa gttttacaaa cctgtaaaca tgtctctgtt
    10861 tttagttggt aattttgatg tggagcgagt acaggactat tttgaaagca aagaactgaa
    10921 agattcagat tttcaggaag tagcaagaga aaagttgttt ttacagcctg taaagccaac
    10981 agatagtatg agaatggaag tatcttctcc caaactagcg attggagtta gaggtaagcg
    11041 agaagtttct gaagcggatt gctatcgaca tcatatttta ttaaaattat tgtttgcaat
    11101 gatgtttggt tggacttcgg atcgttttca aaaatgttat gaatcaggta aaattgatgc
    11161 gtccttatct ctggaagttg aaataacaag tcgctttcat tttgtcatgt tgacaatgga
    11221 tacgaaagag ccagttgctt tgtctcatca atttaggaag gctattcgta attttacaaa
    11281 ggatttagat attacagagg aacatttaga tattatcaaa agagagatgt ttggcgaatt
    11341 tttcagtagc atgaactctc ttgaatttat tgcaacgcaa tatgatgctt ttgaaaatgg
    11401 tgagacaatt tttgatttgc cgaaaatttt acaggaaatt actttagagg atgtccttga
    11461 tgctggacat catttaatag atgatggtga catagttgat tttacaatat ttccatcgta
    11521 gtaacctatc ataatagaca ctagaaagaa gggatgacaa gtatgagaaa aaaaacaatt
    11581 ggagaggttt tacgattagc tagaatcaat cagggattga gtttagatga attgcagaaa
    11641 aagacagaaa tccagttaga tatgttggaa gcaatggaag cagacgattt cgatcaactt
    11701 ccaagtcctt tttacacgcg ttctttcttg aaaaaatatg catgggctgt tgagttagat
    11761 gaccaaattg ttttggatgc ttatgattct gggagtatga ttacttatga ggaagtagat
    11821 gttgatgaag atgagttgac aggtcgtaga cgttcaagta agaaaaagaa gaaaaaaaca
    11881 tcatttttac ctttatttta ttttatcctt tttgctttat cgattttaat ttttgtgact
    11941 tattatgttt ggaactatat tcaaactcaa ccagaggagc cttctctttc taattacagt
    12001 gtggttcaat caacaagttc aactagctct gttccccact cctcaagtag tagttcttct
    12061 agtatagaat cagctataag tgtatcaggc gaaggaaatc atgtagaaat cgcttataag
    12121 acaagtaagg aaacagttaa attgcaattg gcagtttcag atgttacaag ttgggtcagt
    12181 gtttcagaaa gcgaacttga gggcggtgta accttatcgc caaagaagaa aagtgcagaa
    12241 gcaacagttg caactaaaag tcctgtaaca attacgttag gtgttgtaaa aggtgttgat
    12301 ttgacagtag ataatcagac tgttgattta tcgaaattaa cagctcagac tggacaaatc
    12361 actgtaacct ttactaaaaa ttaaggaaaa acgaatgaaa aaagaacaaa ttcccaatct
    12421 cttaacaata ggtcgaattc tctttatacc tatttttatc tttattttaa cgataggaaa
    12481 ttcgatagag agtcatatag ttgcagctat tatctttgct gttgccagta ttaccgacta
    12541 tttagatgga tatttagctc gtaaatggaa tgtggtcagt aattttggta aatttgcaga
    12601 tcctatggcg gataagttac tagttatgtc ggcttttatt atcttgattg agttaggtat
    12661 ggctccggct tggattgttg cagtgattat ctgtcgtgag ttagctgtga caggtttaag
    12721 gcttttattg gttgaaactg gtggaacaat tttagcagca gcaatgcctg gaaaaattaa
    12781 aacttttagt cagatgtttg ccattatttt cttgctatta cattggactt tgcttggtca
    12841 agttctactt tatgtagcct tatttttcac tatctactct ggctatgact atttcaaggg
    12901 tagtgcctat gtatttaaag ggacatttgg ttcgaaatga aatcaataat tgatgtaaaa
    12961 aatctttctt ttcgttataa agaaaatcag aactactacg atgtgaagga tattacgttt
    13021 cacgtgaaac gtggagaatg gctttcgatt gtagggcata atggtagtgg taaatcaacg
    13081 acggttcgat taattgatgg cttactggaa gcagaatctg gagagattgt aattgatggc
    13141 caacgtttga ctgaggaaaa tgtttggaat atacgtcgtc aaatcggtat ggtttttcaa
    13201 aatccagaca atcaatttgt tggagcgact gttgaagatg atgttgcttt tggtttggaa
    13261 aatcagggac tttctcgtca agaaatgaaa aagagagtgg aagaagctct ggctttagtt
    13321 ggcatgttgg actttaaaaa gagagagcca gcgcgtctat caggtggcca aaagcaacgt
    13381 gtggccattg caggtgttgt agccctaaga ccagctattt taatcttaga tgaagcaacg
    13441 agtatgttgg atcctgaggg gcgtagagaa cttattggga cagtaaaagg aattcgaaaa
    13501 gactatgata tgacggtcat ttctattacc catgatttgg aagaagtcgc catgagtgat
    13561 cgcgtattgg tcatgaaaaa aggggaaatt gaatcaacta gtagtccaag ggagcttttc
    13621 tctcgaaatg atttagatca aattggatta gacgatcctt ttgctaatca attaaaaaaa
    13681 tctttgagcc agaatggcta tgatttacct gaaaattatt tgacagaaag tgagctagag
    13741 gataagctat gggaattgct ctagaaaatg tgaattttac atatcaagaa ggtactccct
    13801 tagcttcagc agctttgtcg gatgtttctt tgacgattga agatggctct tatacagctt
    13861 taattgggca cacaggtagt ggtaaatcaa ctattttaca actcttaaat ggtttattgg
    13921 tgccaagtca agggagtgtg agggtttttg ataccttaat cacctcgact tctaaaaata
    13981 aagatattcg tcaaattaga aaacaggttg gcttggtatt tcagtttgct gaaaatcaga
    14041 tttttgaaga aacggttttg aaggacgttg cttttggacc gcaaaatttt ggagtttctg
    14101 aagaagatgc tgtgaagact gcgcgtgaga aactggctct ggttggaatt gatgaatcac
    14161 tttttgatcg tagtccgttt gagctgtcag ggggacaaat gagacgtgtt gccattgcag
    14221 gcatacttgc catggagcca gctatattag tcttagatga gccaacagct ggtctagatc
    14281 ctctagggag aaaagagttg atgaccctgt tcaaaaaact ccaccagtca gggatgacca
    14341 tcgtcttggt aacgcatttg atggatgatg ttgctgaata tgcaaatcaa gtctatgtaa
    14401 tggaaaaggg acgtttagta aaggggggca aaccaagtga tgtctttcaa gacgttgttt
    14461 ttatggaaga agttcagttg ggagtaccta aaattacggc cttttgtaaa cgattggctg
    14521 atagaggcgt gtcatttaaa cgattaccga ttaagataga ggagttcaag gagtcgctaa
    14581 atggatagta tgattttggg gcgttatatc ccaggggatt cgattgttca ccgattggat
    14641 ccacgtagca aattgcttgc tatgatgcta ctgattttga ttgtgttttg ggctaataac
    14701 cccttgacga atctgattct ttttatagcg acagggatat ttattgcctt gtcaggagta
    14761 tctctttcat tttttattca gggcttgaag tctatgtttt tcttgattgc cttcacaact
    14821 atttttcaac tatttttcat ttctaatggg aatgttttat ttgagttttc gtttgtgaga
    14881 atcacggatt atgctttgca acaagctggg attatttttt gtcgctttgt attgattatt
    14941 ttcttttcaa ctttgttaac cttaacaacc atgcctttaa gtttggcatc agctgtcgaa
    15001 gctttattag cacctttaaa gcgtgtgaaa gttccagttc atgaaattgg attgatgctg
    15061 tctatgagtt tgcgttttgt cccaaccttg atggatgata cgactcggat tatgaatgca
    15121 cagaaagctc gtggagtgga ttttggagaa ggaagcatcg ttcaaaaagt aaaggcgatg
    15181 attcccattt tgattcctct ttttgcgaca agtttaaaac gtgcagattc cttggctatc
    15241 gctatggaag cgcgtggcta tcagggtgga aaaggcagaa gtcaataccg acaattgaaa
    15301 tggactctaa aggatacgct gaccattctt gttattctcg tacttggttg ttgtttattt
    15361 ttcttaaaat cttagtagct ttcaggaatt gataaaaagt tactgtaaca gtttttgata
    15421 taaaggtagg aatatgaacc gttttaaaaa atcaaaatat gtcattattg tttttgtcac
    15481 tgttctgctt gtgtcagctc tcttagcgac gacttattca agtacagttg tgacaaaatt
    15541 aggagatgga atctcattgg ttgatagagt tgtacaaaaa ccttttcagt ggtttgattc
    15601 tgtcaaatca gatttggctc atttgacacg aacatataat gaaaatgaaa gtttgaagaa
    15661 acagctttac caattagaag ttaaatcaaa tgaggtggaa agtttaaaga cagaaaatga
    15721 acaactgcgc caattgcttg atatgaagtc taaattgcaa gccacaaaga ctttagcagc
    15781 agatgttatt atgcgttctc cggtatcttg gaagcaggag ttgaccttag atgcaggtag
    15841 atcaaaaggt gcttctgaga acatgttagc tattgcaaat ggtggcttga ttgggagtgt
    15901 ttcaaaagta gaggagaact ctactatagt caaccttctg acaaatacgg aaaatgctga
    15961 taagatttct gttaaaattc aacatggctc tactacaatt tatggaatta ttattggcta
    16021 tgacaaggaa aatgacgttc ttaaaattag ccaattaaat agtaatagcg atattagtgc
    16081 gggagataag gtgactactg gtggattagg aaactttaac gttgctgata ttcctgttgg
    16141 tgaagtggtt gccacaacgc atagtacaga ctatttgaca cgagaagtaa ctgttaaatt
    16201 gagtgcagat actcataatg tagatgtgat agaattagtg gggaattcat aatgagacag
    16261 ttgaagcgag ttggagtatt tttattgctt cctttctttg ttctaattga cgcccatatt
    16321 agccagcttc tgggctcatt tttcccccat gtacatttgg ctagtcattt tctttttcta
    16381 tttctcttat ttgagacgat agaagtatca gagtatctct acctagtcta ttgttttgtt
    16441 ataggcttgg tttatgatgt ttactttttc catctaatag ggattacaac tctcttattt
    16501 atcttattgg gagccttcct tcataaattg aatagtgtta ttttgttgaa tcgttggaca
    16561 agaatgctag ctatgattgt gctgacattc ctgtttgaaa tgggtagtta tcttttggct
    16621 tttatggtag ggttgacagt agatagcatg tcgattttta tagtctatag cttggtaccg
    16681 acgatgattt taaatttttt atggattact gtttttcaat ttatttttga aaaatattat
    16741 ctataagaac gacatataaa tgtaacaaag gcgtaatatt tattaggcct ttttttggta
    16801 tactagtatt gtctttaaaa gaaggagtat ctacgtaata tgaagaaaaa aatcttagcg
    16861 tcacttttat taagtacagt aatggtttct caagtagctg ttttaacaac tgcgcatgca
    16921 gaaacgactg atgacaaaat tgctgctcaa gataataaaa ttagtaactt aacagcacaa
    16981 caacaagaag cccaaaaaca agttgaccaa attcaggagc aagtatcagc tattcaagct
    17041 gagcagtcta acttgcaagc tgaaaatgat agattacaag cagaatctaa gaaactcgag
    17101 ggtgagatta cagaactttc taaaaacatt gtttctcgta accaatcgtt ggaaaaacaa
    17161 gctcgtagtg ctcaaacaaa tggagccgta actagctata tcaataccat tgtaaactca
    17221 aaatcaatta cagaagctat ttcacgtgtt gctgcaatga gtgaaatcgt atctgcaaac
    17281 aacaaaatgt tagaacaaca aaaggcagat aaaaaagcta tttctgaaaa acaagtagca
    17341 aataatgatg ctatcaatac tgtaattgct aatcaacaaa aattggctga tgatgctcaa
    17401 gcattgacta cgaaacaggc agaactaaaa gctgctgaat taagtcttgc tgctgagaaa
    17461 gcgacagctg aaggggaaaa agcaagtcta ttagagcaaa aagcagcagc tgaggcagag
    17521 gctcgtgcag ctgcggtagc agaagcagct tataaagaaa aacgagctag ccaacaacaa
    17581 tcagtacttg cttcagcaaa cactaactta acagctcaag tgcaagcagt atctgaatct
    17641 gcagcagcac ctgtccgtgc aaaagttcgt ccaacatata gtacaaacgc ttcaagttat
    17701 ccaattggag aatgtacatg gggagtaaaa acattggcac cttgggctgg agactactgg
    17761 ggtaatggag cacagtgggc tacaagtgca gcagcagcag gtttccgtac aggttcaaca
    17821 cctcaagttg gagcaattgc atgttggaat gatggtggat atggtcacgt agcggttgtt
    17881 acagctgttg aatcaacaac acgtatccaa gtatcagaat caaattatgc aggtaatcgt
    17941 acaattggaa atcaccgtgg atggttcaat ccaacaacaa cttctgaagg ttttgttaca
    18001 tatatttatg cagattaatt tacagaggga ctcgaataga gccctctttt caggttttac
    18061 cgtgacaatc cctattaaaa attatatcaa aatagcttga aaatattgga aaagtatggt
    18121 agaatgaaaa ttgtcgtgtg aacgataata ctcattcttg atgaattgtg aagcagttgc
    18181 ccttgggtcg ttttgcgagt tgaagtcaag aagaggaaaa aaacaaaaag gagaaatact
    18241 catggcagta atttcaatga aacaacttct tgaggctggt gtacactttg gtcaccaaac
    18301 tcgtcgctgg aatcctaaga tggctaagta catctttact gaacgtaacg gaatccacgt
    18361 tatcgacttg caacaaactg taaaatacgc tgaccaagca tacgacttca tgcgtgatgc
    18421 agcagctaac gatgcagttg tattgttcgt tggtactaag aaacaagcag ctgatgcagt
    18481 tgctgaagaa gcagtacgtt caggtcaata cttcatcaac caccgttggt tgggtggaac
    18541 tcttacaaac tggggaacaa tccaaaaacg tatcgctcgt ttgaaagaaa ttaaacgtat
    18601 ggaagaagat ggaactttcg aagttcttcc taagaaagaa gttgcacttc ttaacaaaca
    18661 acgtgcgcgt cttgaaaaat tcttgggcgg tatcgaagat atgcctcgta tcccagatgt
    18721 gatgtacgta gttgacccac ataaagagca aatcgctgtt aaagaagcta aaaaattggg
    18781 aatcccagtt gtagcgatgg ttgacaccaa tactgatcca gatgatatcg atgtaatcat
    18841 cccagctaac gatgacgcta tccgtgctgt taaattgatc acagctaaat tggctgacgc
    18901 tattatcgaa ggacgtcaag gtgaggatgc agtagcagtt gaagcagaat ttgcagcttc
    18961 agaaactcaa gcagattcaa ttgaagaaat cgttgaagtt gtagaaggtg acaacgctta
    19021 atttatacaa atagtaatta cctaggaggg cggggcttag cccggctctc ctattttcaa
    19081 aaatatagga gaattaaaat ggcagaaatt acagctaaac ttgtaaaaga gttgcgtgaa
    19141 aaatctggtg ccggtgttat ggacgctaaa aaagcgcttg tagaaacaga cggtgacatc
    19201 gaaaaagcga ttgaattgct tcgtgaaaaa ggtatggcta aggcagctaa gaaagctgac
    19261 cgtgttgctg cagaaggttt gactggtgtt tatgttaacg gtaatgttgc agcagttatt
    19321 gaagtaaatg ctgaaactga cttcgttgca aaaaacgctc aattcgttga attggtaaat
    19381 actacagcta aagtcattgc tgaaggaaaa cctgctaaca atgaagaagc tcttgctttg
    19441 ataatgcctt caggtgaaac tcttgaagct gcatacgtat ctgcaacagc aactatcgga
    19501 gagaaaatct cattccgtcg ctttgcattg attgaaaaaa cagacgcaca acactttgga
    19561 gcataccaac ataacggtgg acgtatcggt gttatttcag ttgttgaagg tggagacgaa
    19621 gcacttgcta aacaattgtc aatgcacatc gcagcgatga aaccaacagt tctttcttac
    19681 aaagaattgg atgagcaatt cgttaaagat gagttggcac aattgaatca cgttatcgac
    19741 caagacaacg aaagccgtgc aatggttaat aaaccagctc ttccacactt gaagtatgga
    19801 tcaaaagctc aattaactga tgatgttatt gctcaagctg aagctgacat caaagctgaa
    19861 ttggctgcag aaggcaaacc agaaaaaatc tgggacaaaa ttatcccagg taaaatggat
    19921 cgcttcatgc ttgataatac taaagttgac caagcttaca cacttcttgc acaagtttac
    19981 atcatggatg acagcaagac agttgaagca taccttgaat cagttaacgc ttcggtagtt
    20041 gagtttgctc gctttgaagt tggtgaaggt atcgagaaag ctgcaaacga cttcgaagct
    20101 gaagttgcag ctacaatggc agcagccttg aataactaat aatagaaagg aagcaaattg
    20161 cttccttttt tgtatagggc ttagattttt cctgaaattg ttttttcccc agtctcactc
    20221 gattagaata attaaaacaa aaagccctat aataagggct aagtgcattt aggagactat
    20281 acttcaaatt catagtaagc gcgtcataac aaggtatcta tcctaattcc ccaacttcca
    20341 gtatactaga taaaaaataa ttatcggagg aaaggtattg tactatgata attcaactaa
    20401 gtgatttagg tcaagttcac cttgtttgtg gcaagacaga tatgaggtag ggaatagact
    20461 cattagccta tgtacttaaa acccactttg aattggattc tttctccggt caagtctttc
    20521 tcttttgtgg tggacgtaaa gaccgcttta aagtccttta ctgggatggt caaggatttt
    20581 ggctactata taaacgcttt gagaacggaa aactgacttg gct
//
LOCUS       DAVBCG010000022        19874 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBCG010000022 DAVBCG010000000
VERSION     DAVBCG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.022"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            380..1333
                     /locus_tag="VQ495_000686"
     CDS             380..1333
                     /locus_tag="VQ495_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2530674.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1384..2574
                     /locus_tag="VQ495_000687"
     CDS             1384..2574
                     /locus_tag="VQ495_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2530675.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            2723..3094
                     /gene="rnpA"
                     /locus_tag="VQ495_000688"
     CDS             2723..3094
                     /gene="rnpA"
                     /locus_tag="VQ495_000688"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2530676.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3069..3893
                     /locus_tag="VQ495_000689"
     CDS             3069..3893
                     /locus_tag="VQ495_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2530677.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            3912..4898
                     /locus_tag="VQ495_000690"
     CDS             3912..4898
                     /locus_tag="VQ495_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2530678.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            4949..5572
                     /locus_tag="VQ495_000691"
     CDS             4949..5572
                     /locus_tag="VQ495_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2530679.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPGWQLQQTSQWMNQAQRLRPHFWVYLQRDEQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            5703..7160
                     /locus_tag="VQ495_000692"
     CDS             5703..7160
                     /locus_tag="VQ495_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2530680.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7183..7464
                     /locus_tag="VQ495_000693"
     CDS             7183..7464
                     /locus_tag="VQ495_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2530681.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7541..8026
                     /locus_tag="VQ495_000694"
     CDS             7541..8026
                     /locus_tag="VQ495_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2530682.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8043..8708
                     /locus_tag="VQ495_000695"
     CDS             8043..8708
                     /locus_tag="VQ495_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2530683.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            8712..9575
                     /locus_tag="VQ495_000696"
     CDS             8712..9575
                     /locus_tag="VQ495_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2530684.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9577..10281
                     /locus_tag="VQ495_000697"
     CDS             9577..10281
                     /locus_tag="VQ495_000697"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2530685.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10460..12133
                     /locus_tag="VQ495_000698"
     CDS             10460..12133
                     /locus_tag="VQ495_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2530686.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12245..13336
                     /gene="ulaG"
                     /locus_tag="VQ495_000699"
     CDS             12245..13336
                     /gene="ulaG"
                     /locus_tag="VQ495_000699"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2530687.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13450..15426
                     /gene="tkt"
                     /locus_tag="VQ495_000700"
     CDS             13450..15426
                     /gene="tkt"
                     /locus_tag="VQ495_000700"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2530688.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15543..15842
                     /gene="yajC"
                     /locus_tag="VQ495_000701"
     CDS             15543..15842
                     /gene="yajC"
                     /locus_tag="VQ495_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2530689.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            15889..16317
                     /locus_tag="VQ495_000702"
     CDS             15889..16317
                     /locus_tag="VQ495_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2530690.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16319..16729
                     /locus_tag="VQ495_000703"
     CDS             16319..16729
                     /locus_tag="VQ495_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530691.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17029..19680
                     /gene="adhE"
                     /locus_tag="VQ495_000704"
                     /gene_synonym="adhC"
     CDS             17029..19680
                     /gene="adhE"
                     /locus_tag="VQ495_000704"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2530692.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         6239 a         3558 c         4317 g         5760 t
ORIGIN      
        1 gtgcattggt tagttgttta ctttcttcat aaattttatt gtatcatatt tttgtttcac
       61 aggaagtcta ataaataaaa attctttaaa attttgttgg gaaaatggga aaagatataa
      121 atggaaactt agtataacac ttggaaaact ctgtaaaaat agtaagtcaa ttcatgtccc
      181 gttttttctc tagggaagtt gggcggctat atttatatga ccatttgtcg taataaacat
      241 ccctaccaag ggtcaataga aataaattca acgaaacttt tgctgtcaga tttagatgcc
      301 caatttgtca tttatactct ttcatatagg actttctata aggatggaaa aaagaaggga
      361 aatttgataa gataggaata tggattttga aaaaattgaa caagcttata cctatttact
      421 agagaatgtc caagtcatcc aaagtgattt ggcgaccaac ttttatgacg ccttggtgga
      481 gcaaaacagc atctatctgg atggtgaaac tgagctaaac caggtcaaag acaacaatca
      541 ggcccttaag cgtttagcac tacgcaaaga agaatggctc aagacctacc agtttctctt
      601 gatgaaggct gggcaaacag aacccttgca ggccaatcac cagtttacac cggatgctat
      661 tgctttgctt ttggtgttta ttgtggaaga gttgtttaaa gaggaggaaa ttactatcct
      721 cgaaatgggt tctgggatgg gaattctagg cgctactttc ttgatctcgc ttgataaaaa
      781 ggtggattac ttgggaatgg aagtggatga tttgctgatt gatctggcag ctagcatggc
      841 agatgtaatt ggtttgcagg ctggctttgt ccaaggagat gccgttcgtc cacaaatgct
      901 caaagaaagc gatgtggtca tcagtgactt gcctgttggc tattatcctg atgatgccgt
      961 tgcgtcgcgc catcaagttg cttctagcca agaacatact tacgcccatc acttgctcat
     1021 ggaacaaggg cttaagtacc tcaagtcaga cggatacgct atttttctag ctccgagtga
     1081 tttgttgacc agtcctcaaa gtgatttgtt aaaagaatgg ctgaaagaag aggcgagtct
     1141 ggttgctatg attagtctgc ctgaaaatct ctttgctaat gctaaacaat ctaagactat
     1201 ttttatccta cagaagaaaa gtgaaatagc agtggagcct tttgtttatc cacttgctag
     1261 cttgcaagat gcaagtgttt taatgaaatt taaagaaaat tttcaaaaat ggactcaagg
     1321 tactgaaata taaaatagat tttgttataa tagttgaaaa cgcttaaaaa ggggtatcat
     1381 gttatgacaa aaacaattgc aatcaatgca ggaagttcaa gtttgaaatg gcaattatac
     1441 ttaatgccag aggaaaaagt gttagccaaa ggtttgattg aacgtatcgg tttgaaagat
     1501 tcaatttcaa ctgtaaaatt tgacggccgt tctgaacaac aaattttgga tatcgaaaat
     1561 catacacaag ctgttaaaat tttattggat gacttgattc gtttcgatat tatcaaggct
     1621 tatgacgaga ttacaggtgt tggccaccgt gttgtagcag gtggagaata tttcaaagaa
     1681 tcaacagttg ttgagggaga tgttttagaa aaagttgaag agttgagttt gttggctcct
     1741 ctacacaacc cggccaatgc agcaggtgtt cgtgctttca aggaattgtt gccagacatt
     1801 accagtgtag ttgtttttga tacttccttc cacacaagta tgccagagaa agcttatcgc
     1861 taccctctac caacaaaata ttacacagaa aacaaggttc gtaaatacgg tgctcatggt
     1921 acaagtcacc agtttgtagc aggagaagct gcaaaactct tgggacgtcc attagaagac
     1981 ttgaagttaa ttacctgtca tattggtaac ggaggctcaa ttacagctgt gaaagccggc
     2041 aaatctgtag acacttctat ggggttcact cctcttggtg gtattatgat gggaacgcgt
     2101 acaggggata ttgatccagc tatcattcct tatttaatgc aatatacaga ggattttaac
     2161 acaccagaag atatcagtcg tgttcttaac cgtgaatcag gtcttttggg agtttctgct
     2221 aattctagcg atatgcgcga tatagaagca gctgtagcag aagggaatca cgaggctagc
     2281 ttggcttatg aaatgtatgt tgaccgtatc caaaaacata tcggtcagta ccttgcagtg
     2341 ctaaatggaa cagatgccat tgttttcaca gcaggtgtcg gtgaaaatgc agagaatttc
     2401 cgtcgtgatg taatctcagg gatttcgtgg tttggttgtg atgttgatga tgaaaagaat
     2461 gtctttggcg ttacaggaga catctcaaca gaggcagcta aaatccgtgt cttggttatt
     2521 ccaacagatg aagaattagt cattgcccgt gacgttgaac gcttgaaaaa ataagtgaaa
     2581 ctaaaaaaat attcaataca aggagttggg aaagttattt ttccagcttc tttttctgat
     2641 gaaattgtcc aaaaccttgc tatgattggc ttttttgaaa aatatggtat aatagtagta
     2701 atttaataga tggagttgag ttttgaagaa aaactttcgt gtaaaaagag agaaagattt
     2761 taaggcgatt ttcaaggagg ggacaagttt tgctaatcgc aaatttgtgg tctaccaatt
     2821 agaaaaccag aaaaaccatt ttcgagtagg tctatcagtt agcaaaaaac tggggaatgc
     2881 cgtcactaga aatcaaatta agcgacggat tcggcatatt atccagaatg caaaagggag
     2941 tctggtagaa gatgtcgact ttgttgtcat tgctcgaaaa ggagtcgaaa ccttgggata
     3001 cgcagagatg gagaaaaatc tactccatgt attaaaatta tcaaagattt accgggaagg
     3061 aaatgggagt gaaaaagaaa ctaaagttga ctagtttgct aggactgtct ctgttaatca
     3121 tgacagcctg tgcgactaat ggggtaacta gcgatattac agccgaatcg gctgattttt
     3181 ggagtaaatt ggtttacttc tttgcgaaaa tcattcgctt tttatcgttt gatattagta
     3241 tcggagtggg gattattctc tttacggtct tgattcgtac agtcctcttg ccagtctttc
     3301 aggtgcaaat ggtggcttct aggaaaatgc aggaagctca gccacgcatt aaggcgcttc
     3361 gagaacaata tccaggtcga gatatggaaa gcagaaccaa actagagcag gaaatgcgta
     3421 aagtatttaa agaaatgggt gtcagacagt cagattctct ttggccgatt ttgattcaga
     3481 tgccggttat tttggccttg ttccaagccc tatcaagagt tgacttttta aagacaggtc
     3541 atttcttatg gattaacctt ggtagtgtgg atacaaccct tgttcttccg attttagcag
     3601 cagtattcac ctttttaagt acttggttgt ccaacaaagc tttgtctgag cgaaatggcg
     3661 ctacgactgc gatgatgtat gggattccag tcttgatttt tatctttgca gtttatgcgc
     3721 caggtggagt cgccctatac tggacagtgt ctaatgctta tcaagtcttg caaacctatt
     3781 tcttgaataa tccattcaag attatcgcag agcgcgaggc cgtagtacag gcacaaaaag
     3841 atttggaaaa tagaaaaaga aaagccaaga aaaaggctca gaaaacgaaa taaataagga
     3901 ggaatctggt aatggtagta tttacaggtt caactgttga agaagcaatc cagaaaggat
     3961 tgaaagaatt agatattcca agaatgaagg ctcatatcaa agtcatttct agggagaaaa
     4021 aaggctttct tggtctattt ggtaaaaaac cagcccaagt ggatattgaa gcgattagtg
     4081 aaacgactgt tgtcaaagca aatcaacagg tagtaaaagg cgttccgaaa aaaatcaatg
     4141 atttgaacga gcctgtgaag acggttagtg aagaaaccgt tgaccttggt catgtggttg
     4201 atgctattaa aaaaatagag gaagaaggtc aaggtatttc tgatgaagtc aaggctgaaa
     4261 tcttaaaaca tgaaagacat gccagcacta tcttagaaga aactggtcac attgagattt
     4321 taaatgaact tcaaatcgag gaagcgatga gggaagaagc aggcgctgat gaccttgaaa
     4381 ctgagcaaga tcaaactgaa aatcaagact tgaaagagat gggcttgaag gtcgagcaaa
     4441 gttatgatat tgcccaggtg gctacggatg tgactgccta tgttcaagcg attgtggatg
     4501 acatggatgt tgaagctaca ctttcaaatg attataaccg tcgtagcatc aatctacaaa
     4561 ttgacaccaa cgaaccaggt cgtattatcg gctaccatgg taaagtcttg aaggccttgc
     4621 aactgttggc tcaaaattat ctttacaacc gctattccaa aaccttctac gttacaatca
     4681 atgtcaatga ttatgtcgaa caccgtgcag aagtcttgca gacctatgcg caaaaattgg
     4741 cgattcgtgt tttagaagaa ggacgtagtc atcaaacaga tccaatgtcg aatagtgaac
     4801 gcaagattat ccaccgtatt atttcacgta tggatggcgt gaccagttac tctgaaggtg
     4861 atgagccaaa tcgctatgtc gttgtagata cagaataagt aaaatcaggg ttgacctgat
     4921 tttttgctag ctagaggagg ttaaactaat gttgaataag ataagagact atttagactt
     4981 tgctggtttg cagtaccgta atcctgataa agcgggagca gagcgagaga agatgctggc
     5041 attccgccac aaaggacaag aggcccgaaa ggcttttaca gaactggcca aagcctttca
     5101 agcaagccat ccaggatggc aactccaaca gactagtcag tggatgaatc aggcccagcg
     5161 tttgagacca catttttggg tttatctaca gagagacgaa caagtgacag aacctatgat
     5221 ggccttacgt ttgtatggga catctactga ctttggaatt tctttggaag tcagtttcat
     5281 cgaacgtaag aaggatgagc aaacactggg caagcaggcc aaagttttag acattccaac
     5341 cgttaaaggg atttattatc taacctactc taatggtcaa agtcaacggt gggaggcgaa
     5401 tgaagaaaag cgtcgtactt tacgcgagaa ggtgagaagt caagaagttc gaaaagtttt
     5461 agtgaaggta gatgttccta tgacagaaaa ttcgtctgaa gaagaaatcg tagaaggctt
     5521 attgaagtct tattctaaaa ttcttcccta ttatctagct acgagaaaat aagataattt
     5581 gtaaaacatc ataaatcata cagtccaaga gtgaacagtc cgctgtgtaa ttcttggtct
     5641 ttttgtttgc gctttcgcat tatataataa acttacaaaa acaattcaaa aggagaacaa
     5701 ttatggaagt cgtttcaagt gttctaaatt ggttttctag caatattttg cagaatcccg
     5761 catttttcgt aggtttattg gtgttgatag gatatgcact tttgaaaaaa cctgcccatg
     5821 acgttttttc agggtttgtt aaagcaacag tagggtatat gttgcttaac gtgggtgctg
     5881 gtggtttggt tacaaccttt cgtccaatct tagcagctct taactacaaa ttccaaattg
     5941 gtgcagcggt tatcgaccct tactttggac ttgctgcagc aaacaacaaa attgcagcag
     6001 agtttccaga ttttgttgga actgcaacta cagctctatt gattggtttt ggaataaata
     6061 tcttgctcgt agctcttcga aagattacga aggtaagaac cctctttatt actggtcaca
     6121 tcatggtaca acaagctgca acagtatctc ttatggttct attcttagta ccacaattgc
     6181 gcaatgctta cggtacagca gcgattggta tcatctgtgg actttactgg gcagttagtt
     6241 caaatatgac tgttgaggca actcaacgct tgactggtgg tggcggattt gcgattggtc
     6301 accaacagca atttgcaatc tggtttgtag ataaagtagc aggacgcttt ggtaagaaag
     6361 aagaaagttt agacaatctt aaattaccta agttcctctc aatcttccac gatacagttg
     6421 ttgcatctgc taccttgatg ctcgtattct tcggagccat tcttttaatc ttgggtccag
     6481 acattatgtc taataaagaa gtcatcactt caggaactct attcaatcct gctaaacaag
     6541 atttctttat gtacattatc caaacagcct ttaccttctc agtttacttg ttcgttttga
     6601 tgcaaggtgt ccgaatgttc gtatctgagt tgacaaacgc cttccaaggt atttcaaaca
     6661 aattgttgcc aggttcattc ccagcggttg acgttgcagc ttcttatgga tttggttctc
     6721 caaatgctgt cttgtcagga tttacctttg gtttgattgg tcaattgatt acaattgttt
     6781 tgctcatcgt ctttaaaaat ccgattctta ttattacagg atttgtacca gtgttctttg
     6841 acaatgcagc cattgcggtc tacgctgata aacgcggcgg atggaaagcg gctgttatcc
     6901 tttcctttat atcaggtgtc cttcaagttg ctctaggagc tctttgtgtg gcccttctcg
     6961 atttggcatc ttatggtggc taccatggaa atatcgactt tgaattccca tggcttggat
     7021 ttggatatat cttcaaatac cttggtattg ttggttatgt acttgtgtgt ctcttcttgc
     7081 ttgttattcc tcaacttcaa tttgccaaag caaaagataa agagaaatat tacaacggtg
     7141 aagttcaaga agaagcttag tatctagaaa aggagaaata aaatggttaa agtattagca
     7201 gcgtgcggaa atggaatggg ttcatcaatg gttatcaaga tgaaggttga aaatgctctc
     7261 cgtaagctta atcaaacaga ttttacagtc aattcatgca gtgtcggtga agctaaaggt
     7321 ttagcagtag gatatgacat tgtaatcgct tctcttcatt tgattcaaga attggaaggg
     7381 cgaactaatg ggaagttaat tgggcttgat aacttgatgg atgataaaga aatcaccgaa
     7441 aaactcagtc aagcactaca gtaaaaggtt ggagggggct ggacagaaac tgagagttat
     7501 cgtttctgtc cagccccctc tttaaataaa ggaggcagat atgaatttaa aacaagcttt
     7561 aattgacaat gactcgatcc gactaggttt agaggctaac aattggaaag aagcagtcaa
     7621 ggtagcagta gatcccttga ttgaaagtgg ggcaattttg ccagagtatt acgatgctat
     7681 cattgaatcg actgaagagt atgggcctta ctatatcttg atgccaggta tggctatgcc
     7741 ccacgctaga cctgaagctg gtgtgcaaag tgatgccttt tcattgatta ccttacaaaa
     7801 tcctgttgta ttttcagatg ggaaagaggt atctgttttg ttggcactag cagcaacaag
     7861 ttcaaaaatt cacacaagtg tagccattcc acaaattatt gccctgtttg aattagaaga
     7921 ttctattgca cgtttacagg cttgccagac taaagaagat gtcttggcta tgattgaaga
     7981 atctaaggat agcccttatc tcgaaggatt ggatttggaa agttagaaag aggaataaag
     8041 aaatgacaaa aagaatacct aatttacaag ttgcattaga ccattcagac ttgcaaggag
     8101 cgattaaagc agctgtttct gttggtcagg aagtagatat tatcgaagct ggaactgttt
     8161 gcttgcttca agttggaagt gaactggctg aagtcttgcg tagccttttc ccagataaga
     8221 ttattgtggc agacacaaaa tgtgctgatg ctggtggaac agttgctaaa aataatgcgg
     8281 ttcgtggagc agactggatg acttgtatct gttgtgcaac catccctact atggaagcag
     8341 ctctaaaggc tatcaagact gaacgaggag aacgaggcga aatccagatc gagctttatg
     8401 gcgattggac ttttgaacaa gctcagcttt ggctagatgc aggtatctca caagctattt
     8461 atcaccaatc tcgtgatgct cttcttgctg gtgaaacttg gggtgaaaaa gaccttaata
     8521 aggttaaaaa actcattgac atgggcttcc gtgtatctgt aacaggtggt ctagatgtag
     8581 atactctcaa actctttgaa ggtgttgatg tctttacctt tatcgcaggt cgtggaatta
     8641 cagaggctgc ggatccagca ggagcagcgc gtgccttcaa ggatgaaatc aaacgaattt
     8701 gggggtaaat catggtacgt ccaattggaa tttatgaaaa ggcaacccca acacacttta
     8761 cttggctaga acgtttaaat tttgccaagg agttaggctt tgattttgtc gagatgtcta
     8821 ttgacgaacg tgacgagcgt ttagcaagac ttgactggag taaggaagaa cgcttggaag
     8881 ttgtcaaaga aatctatgaa actggtgttc gtattccttc tatctgtttt tcaggccatc
     8941 gtcgctaccc attgggttca aaagatccag ttctagagga aaaatctcta gaactcatga
     9001 aaaaatgtat cgaattagct caagacttgg gagttcgtac gattcaatta gctggttacg
     9061 atgtttacta tgaggaaaag tcaccccaga cacgccaacg ttttatcaaa aatttgagaa
     9121 aagcctgtga ctgggctgaa gaagctcagg tggtacttgc tattgaaatt atggatgatc
     9181 ctttcatcaa tagcatcgaa aaatatttgg ctatagaaaa agagattgac tctcccttcc
     9241 tctttgtata tccagatatt ggtaatgtgt ctgcatggca taatgatatc tatagtgagt
     9301 tttatcttgg tcatcatgcc atcgcagctc tccatctcaa ggatacttat gcagtgacag
     9361 aaagttcaag gggccagttc cgagatgtac cttttgggca aggttgtgtc aaatgggaag
     9421 aagctttcga tattttaaag gaaaccaatt ataatggacc tttcctaatc gaaatgtggt
     9481 ctgaaaattg tgaaacagta gaagaaacac gcgcagccat tcaagaggcg caagcttttc
     9541 tctatccact cattaagaaa gcaggtttga tgtaagatga atcaagtaat caatgctatg
     9601 cgtaaacgag tctgtgatgc caatcaatca ttgccaaaac atggacttgt caaatttacc
     9661 tgggggaatg tatctgaagt caatcgcgaa ctcggtgtca ttgttatcaa accatcaggc
     9721 gtggattatg acgaattgac acctgaaaac atggtagtga ctgatctaga tggtaagatc
     9781 ctagaagggg atttaagacc atcttccgac ctcccaactc atgtgcaatt atataaggct
     9841 tggtcagaaa ttggtagtgt ggttcacacc cattcgacag aagctgttgc ttgggctcag
     9901 gcaggtcgtg atattccttt ctacggaaca acccatgcag attatttcta cggttcaatc
     9961 ccttgcgccc gtagtttgac caaggacgaa gtagaagtgg cctatgaaaa agatactggc
    10021 ctggttatcg tagaagagtt tgaacatcgc ggacttaacc cggttgaagt accaggaatt
    10081 gttgtacgca atcacggtcc attcacctgg ggcaaaaatc cagagaatgc tgtttatcac
    10141 tctgtcgtac tagaggaagt atcaaagatg aatcgcttta cagaacaaat caatccaaga
    10201 gttggacctg ctccccagta catactagaa aaacactacc aacgtaaaca tggaccaaat
    10261 gcttattatg gtcaaaagta agaacgatga aggaggagaa aaagataaat ttagctcctc
    10321 tttttacatt tgatttttat tgagagtaaa gttggagttg aagtaatttt aaaagatttt
    10381 ttagaaatag cgcttgatat atatatggta aaataaaaag aattgctgtg atatcaatag
    10441 atttggggga ttttttaata tggtactgga taaggcaagt tgtgatttgc ttcaatattt
    10501 gatggatcaa gaaacgtcca aaacgattat ggcgatttcg aaagatttga aagagtcaag
    10561 aaggaaaatt tattatcaca ttgacaaaat caatgctgct ctgggtgacg aggcgcttca
    10621 catcattagt attccacgaa ttggtattca cttaacggaa gagcagagag atgcttgttg
    10681 taaactatta tcggaagtag attcgtacga ttatatcatg agtgcgcatg aacgtatgat
    10741 gataatgtta ctatggatag gtatttctaa agaacgtatt acgattgaaa aattgataga
    10801 gttaacagag gtatctagga atactgttct caatgatttg aatagtattc gttatcaact
    10861 aactttggaa caatatcagg tgatcttgca agtgagcaag tcacagggat accaccttca
    10921 tgcccaccct cttaataaaa ttcagtatct tcaatcgctt ctatatcata tttttatgga
    10981 agaaaatgcc acttttgtat ctattttaga agataagatg aaagagaggt tagatgatga
    11041 gtgtttgctt tctgttgaaa tgaaccaatt ttttaaggaa caggttcctt tagttgaaca
    11101 agatttaggg aagaaaataa accatcatga aataactttt atgttgcagg ttctacctta
    11161 tttgctgtta agctgtcata atgttgaaca gtatcaagaa agacatcagg atatagagaa
    11221 agaattttct ttgataagaa aaagaataga gtatcaggtg tctaagaaat taggagaacg
    11281 gttgtttcaa aagtttgaaa tttctttgtc aggacttgaa gtttctcttg tagctgttct
    11341 cctcctctcc tatcgtaaag atttggatat tcatgcagaa agtgatgatt ttcggcaatt
    11401 aaaacttgct ttagaagaat ttatctggta ttttgaatca caaatccgaa tggagattga
    11461 gaacaaggat gatttgttac gaaatttgat gatccactgt aaagccttgt tatttagaaa
    11521 gacttacggt attttttcta aaaatcctct aacaaaacaa attcgatcca agtatggaga
    11581 attattttta gtcactagaa aatctgcgga aattttagaa ggagcatggt ttattcggct
    11641 aacagacgat gatattgcct atttgacgat tcatattgga ggatttttaa aatatacacc
    11701 atcatctcaa aaaaatatga aaaaagttta tctcgtttgt gatgaaggtg ttgcggtttc
    11761 gagacttttg ctgaaacaat gcaaacttta ttttccaaat gagcaaattg acactgtatt
    11821 tacaacagaa caatttaaga gtgtggaaga tattgcacaa gttgatgtag tgattactac
    11881 taatgatgat ttggatagca gatttccgat tttaagggtt aatcctatcc ttgaagcaga
    11941 agatattttg aaaatgctag actatcttaa acacaatata tttcgtaata agagcaaaag
    12001 tttcagtgaa aatctttcta gtcttatttc gtcttatatt gtagacagca agttggctag
    12061 taagttccaa gaagaggttc aaacacttat aaatcaagaa atagtagttc aagctttttt
    12121 ggaagatatt tgaaggacag tccaatgatg aacacaaacc tgtgtttttc ttggtctttt
    12181 ttagtgtttt gaagggtggt atactaatct caaagataac aattatatcc aaaggaggca
    12241 acatatgcca aacgtcaaag aaattacaag agagtcatgg attttagcca ctttcccaga
    12301 gtggggaaca tggttgaacg aagaaatcga agaagaagtc gtacctgaag gcaactttgc
    12361 catgtggtgg ctaggcaact gtggtacttg gattaagaca ccagctggtg ctaacgttgt
    12421 catggacctt tggtcaaacc gtggaaaatc aaccaaaaaa gtgaaagata tggttcgtgg
    12481 gcaccaaatg gcaaatatgg caggtgttcg taagctgcaa ccaaacttgc gtgttcagcc
    12541 aatggttatc gatccatttg ctatcaacga actagactat tacttagttt cacacttcca
    12601 cagtgatcat atcgacccat acacagctgc agcaattctc aataatccta agttagagca
    12661 tgttaagttt atcggtcctt accactgtgg acgaatctgg gaaggatggg gtgttccaaa
    12721 agaacgtatc atcgttgtta aaccaggtga cactatcgaa ttaaaagata tgaagattca
    12781 tgcagtagaa tcatttgacc gtacttgctt ggtaactctc ccagtgaacg gtgctgatga
    12841 gacaggcggt gaacttgctg gcttggctgt tacagatgaa gaaatggctc aaaaggctgt
    12901 taactatatc tttgaaacac caggtggaac catctatcat ggtgcagatt ctcacttctc
    12961 aaactatttt gcaaaacatg gtaaagactt taaaattgat gttgctttga ataactatgg
    13021 tgaaaatccg gtaggtatcc aagacaaaat gacatctatc gaccttcttc gtatggcaga
    13081 aaatctgcgt accaaagtca ttatcccagt tcactatgat atctggtcta acttcatggc
    13141 ttctactaat gagattctag aactttggaa aatgcgaaaa gatcgcttgc aatacgattt
    13201 ccatccattt atctgggaag ttggcggtaa gtacacttat cctcaagatc aacacttagt
    13261 agaataccat catccacgtg gttttgatga ttgttttgaa caagactcta acattcaatt
    13321 taaagctttg ctataatata aaaatattct ttggaggtta tccgtatgtt gcatgatacg
    13381 gataattttc atataatagt aaaatagaat gtgtgattca ataatcacct caaatagaaa
    13441 ggaaattcta tgtcaaatct atctgttaat gcaattcgtt ttctaggtat tgacgccatt
    13501 aataaagcca actcaggtca tccaggtgtg gttatgggag cggctccgat ggcttacagc
    13561 ctctttacaa aacaacttca tatcaatcca gctcaaccaa actggattaa ccgcgaccgc
    13621 tttattcttt cagcaggtca tggttcaatg ctcctttatg ctcttcttca cctttctggt
    13681 tttgaagatg tcagcatgga tgagattaag agtttccgtc aatggggttc aaaaacacca
    13741 ggtcacccag aatttggtca tacggcaggg attgatgcta cgacaggtcc tctagggcaa
    13801 gggatttcaa ctgctactgg ttttgcccaa gcagaacgtt tcttggcagc caaatataac
    13861 cgtgaaggtt acaatatctt tgaccactat acttacgtta tctgtggaga cggagacttg
    13921 atggaaggtg tctcaagcga ggcagcttca tacgcaggct tgcaaaaact tgataagttg
    13981 gttgttcttt atgattcaaa tgatatcaac ttggatggtg agacaaagga ttcctttaca
    14041 gaaagtgttc gtgaccgtta caatgcctac ggttggcata ctgccttggt tgaaaatgga
    14101 acagacttgg aagccatcca tgctgctatc gaaacagcaa aagcttcagg caagccatct
    14161 ttgattgaag tgaagacggt tattggatac ggttctccaa acaaacaagg aactaatgct
    14221 gtacacggcg cccctcttgg agcagatgaa actgcatcaa ctcgtcaagc cctcggttgg
    14281 gactacgaac catttgaaat tccagaacaa gtatatgctg atttcaaaga acatgttgca
    14341 gaccgtggcg catcagctta tcaagcttgg actaaattag ttacagatta taaagaagct
    14401 catccagaac tggctgcaga agtagaagcc atcatcgacg gacgtgatcc agtcgaagtg
    14461 actccagcag acttcccagc tttagaaaat ggtttttctc aagcaactcg taattcaagt
    14521 caagatgcct tgaatgttgt ggcagctaaa ttaccaacct ttctaggtgg atcagctgac
    14581 cttgctcact caaacatgac ttatatcaag acggatggac ttcaagacga tgccaatcgc
    14641 ttgaatcgta acattcagtt tggtgttcgt gaatttgcaa tgggaacaat cttgaacggg
    14701 atggcccttc atggtggact tcgtgtatac ggtggaactt tcttcgtctt ctctgactat
    14761 gtgaaggcag ctgtccgctt gtcagcctta caaggacttc ctgtgactta tgtctttacc
    14821 catgattcaa tcgcagttgg ggaagatggt ccgactcatg aaccagttga gcatttagca
    14881 ggtcttcgtg ctatgccaaa tctaaatgtt ttccgtccag cagatgcgcg tgaaacgcaa
    14941 gcagcttggt accttgcagt gacaagtgag aaaacaccaa ctgcccttgt cttgacacgt
    15001 caaaatttga ctgttgaaga tggaacagac ttcgacaagg ttgctaaagg tgcttatgtt
    15061 gtatatgaaa atgcagccga ctttgatacc atcttgattg cgacaggttc agaggttaat
    15121 cttgctgtct cagctgctaa agaattggct agtcaaggcg aaaaaatccg cgtagtcagc
    15181 atgccatcta cagatgtctt tgataaacaa gatgcagctt acaaggaaga aattcttcca
    15241 aatgcagtcc gccgtcgtgt tgcagtcgaa atgggtgcaa gtcaaaactg gtacaaatat
    15301 gttggtctcg atggtgccgt tctaggtatt gataccttcg gagcctctgc cccagcacca
    15361 aaagtattgg cagaatatgg ctttactgta gaaaatcttg taaaagttgt tcgaaacttg
    15421 aaataatcct aaaaatcagg gcgtaagctc tggtttttct taccagaaaa gtaaggtaca
    15481 atcttgtaaa agtagctgaa atttgatata gtagtcctat gtaaaagaca aaggagaaaa
    15541 caatgaatcc aaatattact tttttaatca tgcttgtagg tatgatggcc ttgatgttct
    15601 ttatgcaacg ttctcaaaag aaacaagctc aaaagcgtat ggaaagctta aataaactac
    15661 aaaaaggcta tgaagtgatt acaatcggtg gactttatgg aacagtcgat gaagtagata
    15721 cggagaaggg aacaatagtt cttgacgtag agggagttta cttgactttt gaactagctg
    15781 ctatcaagac agtattaccg ctgaaagaaa cagcttcact agaaggcgca attgaaaaat
    15841 aagacgggat cacgaactcc cgtttttcta taaaagaaag gaaatgggat gaaaaaatta
    15901 gtctttgtct gtctgggaaa tatttgccgt agccctatgg ccgagtttgt tatgaaatca
    15961 atgacagata actacgaaat ccaaagtcga gcaacttcct cttgggaaca tggcaatccg
    16021 attcataagg ggactcaggg aatttttcaa gagtatgaga ttccttatga caagaacaag
    16081 acatcgcttc agattagtaa ggaagatttt gaagcctttg attatattat cggaatggac
    16141 gcttcaaata tttctgactt acgtcagatg tgtccagtag actgtcaaga taagatttac
    16201 tcattttcat ctgaaagtgt tccagaccct tggtatacag gagattttga agaaacctat
    16261 cgacgtgttc aagagggctg tcaagcttgg ttagaacgtt tagaaaagga gagttaaggt
    16321 ggaaaatctt acgaattttt acgaaaagta tcgtgtctat ctgactcgtc cacgtttaga
    16381 gcttttggca gtagttacca ttgttttctg tgctgtactc gtcttttttc taaatattcc
    16441 aggaaaaggt gtcttaaaac tcgataatgg aacgattgtt tatgatggca gtcttgtccg
    16501 cggtaaaatg aatggccaag gtaccattac cttccaaaat ggagaccaat atacaggtgg
    16561 cttcaacaat ggagccttca acggaaaagg tacctttcaa tctaaagaag gctggaccta
    16621 cgaaggtgat tttgtaaatg gtcaggctga aggaaaaggg aaactaacaa cagaacaaga
    16681 agtcgtttat gaaggaactt ttaaacaagg cgtttttcaa caaaaataaa gcctccttat
    16741 caaaggaggt attattagaa tcgcaaggta agcgcttacc tgcaaatccc tttctttcca
    16801 aatccctctt ccaagcaagt ttgtgaaata aaaaatattt gaaataaatt tcacaaactt
    16861 caaagataaa acctgataag aaaagaaaat gagaaaagtt tcacaagagt ttaaaaattc
    16921 tttgtgaaat gtttatgaaa ctgtttacaa agttgaaata atgcgttata ataaacttgt
    16981 gaaaaaatta acaaaggata tcgttccttg aaagctatgg aggaaaatat ggctgataaa
    17041 aaaactgtga caccagagga aaagaaactc gttgctgaaa aacacgtaga tgagttggtt
    17101 caaaaagctc tagttgccct tgaagaaatg cgtaaattgg atcaagaaca agttgactac
    17161 atcgttgcca aagcatcagt agcagctttg gatgcccacg gagaattggc tttacatgcc
    17221 tttgaagaaa caggacgtgg tgtatttgaa gataaagcaa ctaagaactt gtttgcttgt
    17281 gaacacgtgg taaacaacat gcgtcacact aaaacagttg gcgttatcga agaagatgat
    17341 gtaacaggat tgaccctgat tgctgaacca gttggtgttg tttgtggtat cactccaaca
    17401 acaaacccaa catcaacagc gatcttcaaa tcattgattt cattgaagac acgtaatcca
    17461 atcgtctttg ccttccatcc atcagctcaa gaatcatcag ctcatgcagc tcgtatcgtc
    17521 cgcgatgcag ctatcgcagc tggtgctcct gaaaactgtg tacagtggat cactgaacca
    17581 tctatggaag caactagtgc ccttatgaac cacgaaggta ttgcaacaat ccttgcaaca
    17641 ggtggtaatg ccatggttaa agccgcatac tcatgtggta aaccagctct tggggtaggt
    17701 gccggaaacg ttccagctta tgttgaaaaa tcagcaaaca ttcgtcaagc agcacacgat
    17761 atcgtcatgt ctaaatcatt tgataacggt atggtctgtg catctgaaca agcagttatc
    17821 attgataaag aaatttacga tgaatttgta gcagagttca aatcttacca cacttacttt
    17881 gtaaacaaaa aagaaaaagc tcttcttgaa gagttctgct tcggcgtcaa agcaaacagc
    17941 aaaaactgtg ctggtgcaaa attgaacgct gacatcgttg gtaaaccagc aacttggatt
    18001 gcagaacaag caggatttac agttccagaa ggaacaaaca ttcttgctgc agaatgtaaa
    18061 gaagttggcg aaaatgagcc attgactcgt gaaaaattgt caccagttat tgcagttttg
    18121 aaatctgaaa gccgtgaaga tggtattact aaggctcgtc aaatggttga atttaacggt
    18181 cttggacact cagcagctat ccacacagct gacgaagaat tgactaaaga atttggtaaa
    18241 gctgttaaag ctattcgtgt tatctgtaac tcaccttcta cttttggtgg tatcggggac
    18301 gtttacaatg ccttcttgcc atcattgaca cttggatgtg gttcttacgg acgcaactca
    18361 gttggggata acgttagtgc cattaacctc ttgaatatca aaaaagtcgg aagacggaga
    18421 aataacatgc aatggatgaa acttccttca aaaacatact ttgaacgtga ttcaattcaa
    18481 taccttcaaa aatgtcgtga cgttgaacgt gtcatgatcg ttactgacca tgccatggta
    18541 gagcttggtt tccttgatcg tatcatcgaa caactggacc ttcgtcgcaa taaggttgtt
    18601 taccaaatct ttgcggatgt agaaccggat ccagatatca caactgtaaa ccgtggtact
    18661 gagattatgc gtgccttcaa accagatacc atcatcgcac tcggtggtgg gtctccaatg
    18721 gatgctgcca aagtaatgtg gctcttctac gagcaaccag aagtggactt ccgtgacctt
    18781 gtccaaaaat tcatggatat ccgtaaacgt gccttcaagt tcccattgct tggtaagaag
    18841 actaaattca tcgcgattcc aactacatct ggtacaggat ctgaagtaac accatttgcc
    18901 gttatctctg ataaagcaaa caaccgtaaa tacccaatcg ctgactactc attgacacca
    18961 actgtggcaa tcgtagatcc tgctttggta ttgacagttc caggatttgt tgctgctgat
    19021 actggtatgg acgtattgac tcacgcgaca gaagcatacg tatcacaaat ggctagtgac
    19081 tacactgatg gtttagcact tcaagccatt aaattggtct ttgaaaatct cgaaagctca
    19141 gttaagaatg cagacttcca ctcacgtgag aaaatgcata acgcttcaac aatcgctggt
    19201 atggcctttg ccaatgcctt cctaggtatt tctcactcaa tggcccataa gattggtgcg
    19261 caattccaca caatccacgg tcgtacaaat gctatcttgc ttccatacgt tatccgttac
    19321 aacggtacac gtccagctaa gacagcaaca tggcctaagt acaactacta ccgtgcagat
    19381 gaaaaatacc aagatatcgc acgcatgctt ggacttccag cttctactcc agaagaaggg
    19441 gttgaatctt acgcaaaagc tgtctacgaa ctcggtgaac gtattgggat ccaaatgaat
    19501 tttagagacc aaggaattga cgaaaaagaa tggaaagaac attctcgtga attagccttc
    19561 ctggcttatg aagaccaatg ttcaccagct aacccacgtc ttccaatggt agaccatatg
    19621 caagaaatca tcgaagatgc atactatggc tacaaagaaa gaccaggacg ccgtaaataa
    19681 ttgtttatca gtctagaagc aagacaaaaa ctcaatttga gggaaagatc cagtaatttt
    19741 tctatgataa aaggcatcct atcaaggttt ttgaacacct gataggatgc ctttttatga
    19801 tattgaggcc tttttgccct ttttgaaaaa ctagaataga aacaaaatat ataatagatt
    19861 gaaactggaa tagt
//
LOCUS       DAVBCG010000023        19760 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBCG010000023 DAVBCG010000000
VERSION     DAVBCG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.023"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(46..1458)
                     /gene="sufB"
                     /locus_tag="VQ495_000705"
     CDS             complement(46..1458)
                     /gene="sufB"
                     /locus_tag="VQ495_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2530693.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(1508..1948)
                     /locus_tag="VQ495_000706"
     CDS             complement(1508..1948)
                     /locus_tag="VQ495_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2530694.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(1935..3161)
                     /locus_tag="VQ495_000707"
     CDS             complement(1935..3161)
                     /locus_tag="VQ495_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2530695.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(3172..4434)
                     /gene="sufD"
                     /locus_tag="VQ495_000708"
     CDS             complement(3172..4434)
                     /gene="sufD"
                     /locus_tag="VQ495_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2530696.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(4462..5232)
                     /gene="sufC"
                     /locus_tag="VQ495_000709"
     CDS             complement(4462..5232)
                     /gene="sufC"
                     /locus_tag="VQ495_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2530697.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(5382..5576)
                     /locus_tag="VQ495_000710"
     CDS             complement(5382..5576)
                     /locus_tag="VQ495_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2530698.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(5604..7259)
                     /gene="dnaX"
                     /locus_tag="VQ495_000711"
     CDS             complement(5604..7259)
                     /gene="dnaX"
                     /locus_tag="VQ495_000711"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2530699.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(7259..7756)
                     /locus_tag="VQ495_000712"
     CDS             complement(7259..7756)
                     /locus_tag="VQ495_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2530700.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            complement(7842..9044)
                     /gene="rpsA"
                     /locus_tag="VQ495_000713"
     CDS             complement(7842..9044)
                     /gene="rpsA"
                     /locus_tag="VQ495_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2530701.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(9206..9277)
                     /locus_tag="VQ495_000714"
     tRNA            complement(9206..9277)
                     /locus_tag="VQ495_000714"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9243..9245),aa:Gln,seq:ttg)
     gene            complement(9287..9367)
                     /locus_tag="VQ495_000715"
     tRNA            complement(9287..9367)
                     /locus_tag="VQ495_000715"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9331..9333),aa:Tyr,seq:gta)
     gene            complement(9417..9647)
                     /locus_tag="VQ495_000716"
     CDS             complement(9417..9647)
                     /locus_tag="VQ495_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2530702.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(9815..10459)
                     /gene="pcp"
                     /locus_tag="VQ495_000717"
     CDS             complement(9815..10459)
                     /gene="pcp"
                     /locus_tag="VQ495_000717"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2530703.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(10474..11397)
                     /locus_tag="VQ495_000718"
     CDS             complement(10474..11397)
                     /locus_tag="VQ495_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2530704.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(11394..12083)
                     /locus_tag="VQ495_000719"
     CDS             complement(11394..12083)
                     /locus_tag="VQ495_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2530705.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(12148..12852)
                     /locus_tag="VQ495_000720"
     CDS             complement(12148..12852)
                     /locus_tag="VQ495_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2530706.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSDM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            complement(12908..13930)
                     /locus_tag="VQ495_000721"
     CDS             complement(12908..13930)
                     /locus_tag="VQ495_000721"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2530707.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(14062..16533)
                     /gene="parC"
                     /locus_tag="VQ495_000722"
     CDS             complement(14062..16533)
                     /gene="parC"
                     /locus_tag="VQ495_000722"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2530708.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(16953..18896)
                     /gene="parE"
                     /locus_tag="VQ495_000723"
     CDS             complement(16953..18896)
                     /gene="parE"
                     /locus_tag="VQ495_000723"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2530709.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            19032..19673
                     /gene="plsY"
                     /locus_tag="VQ495_000724"
     CDS             19032..19673
                     /gene="plsY"
                     /locus_tag="VQ495_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2530710.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         5678 a         4496 c         3565 g         6021 t
ORIGIN      
        1 gctgacgtgg tttgaagaga ttttcgaaga gtataaaatc aaattttatc caactgatcc
       61 ctccatttca tagctaatca agcggttcag ctcaactgcg tattccattg gaagttcttt
      121 tgtaaagggt tctacaaatc ccatgacaat catttcagtt gcctcagatt ctgacaatcc
      181 acggctcatg agataataca attgctcttc tgaaatctta gatactttgg cttcgtgttc
      241 caaagccact tgcgagttgt gaatttcatt aaatggaata gtatctgatg ctgacaagtc
      301 atccatgata atggtatcac attcaatgtg ggaaacagat ttcttagagt tcttgttaaa
      361 ggtgacttgt ccacggtagt caacctttcc tccaccttta gcgatggatt tagacacaat
      421 agacgagctg gtatgtggag cattgtgaat catcttagca cccgtgtctt ggtgttgccc
      481 tgcattagca aaggcgatag agagcatggt accacgcgct ccttctccat caagataaac
      541 agatggatat ttcatagtcg ttttggcacc caagtttcca tcaatccact caacagtggc
      601 atccttttga gccttagcac gctttgttac caagttatag acgttatcag accagttttg
      661 gatagttgta taacgcatat aagctccgtc caaagcaaaa atttctacaa tggcagcgtg
      721 caagctattg cttgaatatg ttggtgctgt acatccttct acgtagtgga cgcttgctcc
      781 ctcatcaacg ataatcaagg tacgttcgaa ctgacctata ttttcgttat tgatacggaa
      841 ataagtttga agtggaatat ctaccttgac accttttggc acgtagataa aagttccacc
      901 cgaccatact gctgagttga gggctgccaa cttgttatct gtcggcggta ccaacttcgc
      961 aaagtattgt ttaaataagt ctgggtattc cttgagtgcg gaatctgtat ctgtaaagat
     1021 aatacctaat ttttggaact cttccttcat gttgtggtaa accacttctg actcgtactg
     1081 ggcagaagcc cctgctaaat aagcacgttc agcttctgga atcccgatac gttcaaaggt
     1141 ttctttaatc ttttcaggta catcatccca agaacgggct ggtttgtcag atggtttttg
     1201 gtagtagatt aagtcatcaa agtcaatctc tgacaagtct gctccccaag tttgcatggg
     1261 catttttttg aaggtttcat aagacttcaa acggaactcc aacatccact caggctcacc
     1321 cttagcagca gataattcac gaataacacc ttcgttgagt ccttttcctg tcgataagac
     1381 aggctctaca tcgtcatgga aaccaaattt atattcacca aggtcaattg gttttggttc
     1441 tactctttct tcagccataa tatcctttct tcatttctaa taattcataa gacaaaagaa
     1501 acttgtctta ctgtttttct tgattttcaa ttgttttctt aagggcattc caagctaggg
     1561 ttgcacactt gattctttga gggaatttgg caacacctga caagaatgcc gcgtctccaa
     1621 gttggtcttg acgctcatct ttttgccctt gaaccatttc agaaaaaata gtcgccagtt
     1681 ctaaaatttc ttgtttggtt tttcctaaaa cggcatctgt catcatacta gcagaagcag
     1741 ttgaaatcgt gcatcctgaa tttagaaaag caatatcttc caaacggtcc tctgcatcaa
     1801 acttgacaga gaggttgatg acatccccac aagtcggatt gttgagactg atttgctcag
     1861 cgtcttctaa cttcccttgg tgatgtggat ttttcgaatg gtctgctacc actgccatat
     1921 aaaggctatc tagtttagaa agtgccattg aaaaactcct ttgtcttttg tagggcatcg
     1981 actagtttgt cgcaatctgc cttggtatta tagatataaa aacttgcacg agctgatgct
     2041 gggacttcca aatactgaag caagggttgc gcacaatggt gaccagcacg aacagccact
     2101 ccttcataat ccagagccgt cgcaagatcg tgaggatgga gatcacctag gttaaaggca
     2161 ataacacccg aacgttgagc caaatcctga gaaccgtaaa tggtcaatcc ctcaattgcc
     2221 tgcagttttg gatagacgta cgcaatcaat tcctgttcat gagcttcaac ggcatccata
     2281 ccaatctttt ccagataatc aactgcagtc gcaagtccaa tagctcctgc catatttggc
     2341 gttccagcct caaatttcca aggcaattcc ttccaactag cggattgctc gtagacaaaa
     2401 tcaatcatct cgccgccaaa ttctactgga gacatttgct caagatactt ttctttgccg
     2461 taaaggacac cgataccagt cggaccagcc atcttgtgac ccgaaaaggc gaaaaagtcc
     2521 agatccaagt cctggacatc aatcttcata tgaggtgtag attgagcacc atccactacc
     2581 atgattgccc caacttggtg ggctaattga gtgatttcct tgatcggatt gaccacacca
     2641 agaacattgg aggcatgagc tagggaaaca aatttaacct tatcagtcaa tttagctcgc
     2701 aaatcctcca tatccaaggc accgtcttta agatagacat agacaagctc tgctccagtc
     2761 tttcgacaag cttcctgcca tggaatgata ttagaatggt gttccattac tgaaatcaag
     2821 acctggtctc cctcagtgag aatttcctca gcaaagcgtg ccacccagtt aaggctggtt
     2881 gtcgttcctc tggtaaagag aacttccttt gtagagcctg cattaataaa cttacgaatg
     2941 gtttcacgag cagcttcata agaagctgtc gctcgttccg ctaaggtatg gacaccacgg
     3001 tgaacattgg cattgtcctg ctcatagtaa ctgttaatag ctttcagaac tactagtggt
     3061 ttttgtgtcg tcgcagcatt gtccagatag accagaggtt catcattgac aatctgatct
     3121 aaaattggaa aatccttgcg aatcgcttct acatctaaca taggcttccc cttagcgttt
     3181 tgacaatttc tcttcgatag ttgcaatcat ttcatcacga acttccttga ctggaatctc
     3241 cacgataaca gatccaagga aaccacgaac aaccaaacgc tctgcagttg ccttatccaa
     3301 tccacgactc atgaggtaat acatgtcttc tggatcaact tgtccgatag acgctgcgtg
     3361 tcctgcagtg acatcatttt catcaatcaa aagaattggg ttagcatctg aacgcgcttg
     3421 gtctgaaagc atgagaacac ggctctcttg ttgggcatct gctcccttag cacccttgat
     3481 gatgtggccg ataccattga aagtcaaagt tgctttttca aggataaccc catgttgtag
     3541 gatatttccg atagagttgc agccatagtt agttacacga gtatcaatcc cttgtacctg
     3601 acgaccactt gaaagagcta caaccttgag gtcagcatag ctaccattac caatcaagtc
     3661 actatcaaaa tcagcaacga catttccttc gttcatgaca ccgatagccc agtcaatact
     3721 tgcatcgttg cctaatttac cacgacggct aatgtaggca gtgacgtttt cacctagacg
     3781 gtcgatagca gcaaacttga cttgcgcacc tgaacgtgca atcacttcta ctgtgatatt
     3841 agcagttgct ttggcacttc cttcaccgcg tgactctaaa cgctccagat aattaatctt
     3901 agaattttta ccagcgataa tcataatatg cttgttaaac ggcacattgc tatcgctatc
     3961 ttggtagaaa attccttcaa ttggctctgt gatttctacg ttatctggaa tatagagtac
     4021 agcaccactg ttaaagtaag ctgtgtggta agccgccaac ttgtcatcat catacttaac
     4081 agatgacatg aagaattctt cgatcagctc tggaatttct tctaaagctg agtgaaagtc
     4141 tgtgaagaca acaccctgtt cagctaactc aactggagtt tgttcgaaaa cagtttgagt
     4201 tcctacttgc accaacttca agtgatgatc taaagctgtg aaatctggaa catttgctga
     4261 tggctcattt tctgtaatcg ttccatcacc cagattccaa cggtggaatt tgacacgctc
     4321 aataactggt aattccaaag tctcaatctt gtcaaaagct ttttgacgga gatcagccaa
     4381 ccagcttggt tcagcgtgca tttctgaaaa aagtttaata ttttctctag tcatttactt
     4441 ctcctaaaag atacgaggaa attacaattc ttccttgtag tcgtagccaa gttcttcagc
     4501 taattttgcg tatccttcac gttccaaacg cgcagccaat tctggaccac cagaaaggac
     4561 aacacgacct tccatcatca cgtgtaccac atcaggtgtg atatagttca aaagacgttg
     4621 gtagtgagtg atgatcatag caccaaaacc ttcaccacgc atggcattga cacctttaga
     4681 cacaacttta agagcgtcaa tatcaagacc tgagtcaatc tcgtccaaaa gagcaaatgt
     4741 tggctccaac atcaaaagtt gaagaatttc attgcgtttt ttctcaccac cagagaagcc
     4801 ttcgttgagg taacgctctg ccatttcttc tttcatgttg agcaattcca ttttttcatc
     4861 tagcctagta ataaactcac gaactgaaat cttctcatca tcttctttac ccgcattcat
     4921 agcggcacga agaaactcag cattggtaat tccagggatt tctgatgggt attgcatagc
     4981 aaggaaaagt cccatacgcg cacgctcatc cacttccaac tcaaggatgt ttacgccatc
     5041 aaacaaaact tcacctttag ttacttcata gtttggattt cccatgatag cggcagaaag
     5101 agtcgattta cctgtaccat ttggtcccat gatagcggca atttctcctg ttttcagggt
     5161 caggttaacc ccttttaaaa tttcttttcc ttcaatctca acgtgaagat ctttgatctc
     5221 taatactgac atgatagttc ctttcttttt caaaactttt acagtactta ctagaaaaac
     5281 gtctgatttc cccagaaata gagcaaaaat tctataaata tacttttcta gtataacaaa
     5341 aaaaagccta aaaggctttg attttgtctg gaagctgagg gctagtcttt cctattaaaa
     5401 tgctgatcga tgacatcaac gattttgccc atcacataac tgactcggaa atttctaaag
     5461 agtaaaattg cccaaaaggc tgccataata tagcggccag tcatccaggc ttcattgaaa
     5521 aaataggtct cagcagctgt aaacagcgcc ataataataa attgttgttt attcataaac
     5581 ttattgtatc acgaaatctt tctttagtct tcctctacct tcactttatc agccaaaaat
     5641 tcaaatcctt ctggaatcag gctttcttct acttcttttt cagtttgaga agatttggct
     5701 ttggctgaaa aggctgcgcg aacttctttc cattcctcca tggaaatagc taaaatctca
     5761 ggtgaaaaac ctgccgcctg actgaggata ttgccaaaca tggtattgag attgtctcgt
     5821 ttcatagttt gaccagcatt gaagttagac tcaaaagcaa gaatagcatg gtgttcattg
     5881 gcagcaaccg gttgagaacc aactagcaga gccttgtccg gcccacctag actttcaatt
     5941 acctctcccc aggcattctg caaacgaatt agattttgac gtgctaaatc aggattttcg
     6001 acggcctctt gtaagataga ttgcacttta ttgcgatcga cacgatagac tgttttgccc
     6061 gtagctggtc gactaggagc tggtgcaact tgtttaggaa ccgcacctac attagaaagc
     6121 tcttgtttga gacgggcaac ttcctgtctc agcgtagcaa tttcattttc aaccgctcct
     6181 gatagagctg gttcgggctt gatttccgcc aaacggacgg tcatcatttc agcataaatc
     6241 ttgggctgca aactagactt aatatctgct aaactcactg ttgctaagcg aatcatttca
     6301 aacagatttt tttgaggaag tgccaaattt tctacaaaga ctgaactatg atgagtattt
     6361 tctccccctg tttgaacaat taacaagtct cttaaatagt gcaaaagatc ggtcacaaaa
     6421 cgagtcatgc tcttaccatt gtcaaaaaga agattcaagc aagacaaagc tttgggaaca
     6481 tcctgttgag acaaggccgc cacataatca tccaaggctg ataggctaat agtgccagta
     6541 atttcttcag agatagcagt cgtcagctca tttccctgtg tcaaactcag ggcttgatcc
     6601 aaaatagaca aggcgtcccg cattccacct tccgcccgtc tggcaatgat ttccacagcc
     6661 tctggttcag aactgatatt ttctttttct aagatatagt gaatatgttc cttaatatcc
     6721 tgtgtcttaa ttgatttaaa ctcaaaacgt tgcacacggg atagaatagt agcaggaatc
     6781 ttgtgtaatt cagtagtggc caaaataaag actacattct gtgttggttc ttccagcgtc
     6841 tttaggaggg cattaaaagc ccctgtagac agcatgtgaa cctcatctat gatataaacc
     6901 ttataacgag caaggctagg cgcataggta gatttatcac gaatttcgcg aatttcatct
     6961 accccattat tagaagctgc atccatttca atgacatctt ctaaactacc gtccgtcact
     7021 gcttgacaaa tatagcagtt attgcaaggt tcgccaccca cttgattggg acagttcata
     7081 gccttggcaa agattttagc aacactggtt tttcccgttc cacgaggacc agaaaaaaga
     7141 taagcgtgac ttattttctc ttgctccacc gcttgtttaa gagtcttagc cacaacttct
     7201 tgaccaacta actgggagaa gttttgactt ctatattttc gataaagtgc ttgatacatt
     7261 aagatttttc ctcaaacatc gtaaagtccc atgttgtctt ttcaagcaaa atagcgacaa
     7321 attgttccaa ataatctcga tccatagcat cgtaatcctc aatctctgaa gaatccagat
     7381 ccagaactcc aagtaactga ccattcttca tcatcggcac cacaatttca cttttagcta
     7441 gactatcaca agaaatatag ttgagatagg tcgtcacatc tccaacaata acagtttcct
     7501 gaaagtgagc tgcctcacca caaacaccct tgcctagtgc aatacggatg caggaaacac
     7561 ctccttggaa gggacctaaa accaattcct ttccatcgaa caaataaaag cctgcaaata
     7621 cggtattagg aaaacgtgat tttataagag cactggcgtt ggaaagatta gccaaaacat
     7681 tggtttcgcc ttccaatagg aaggacaatt cttcatttaa catttgataa cgtgattgtt
     7741 tttctgattt taacatagaa ctattatatc aaaaaaggag cagagacaaa agaaaaatcc
     7801 cttgtttagt aaacaaagga ttttgtgaat tttcaatttg attaaagttc gatatcacca
     7861 aacaaatcag ccattgaaaa tcctgtttgt gtttctggaa gttcgaaatc acgcttttct
     7921 tgacgtcttg gacgacgtgg acgagcagca cgtttttctt ctttttgtcc ttcttcttgg
     7981 gctggacgtt cttcaagagc tttaatagaa agtgacacgc gttctgcatc tgcgttaact
     8041 tcaagaactt taacttgaac ttcttgacca actttaagag cttcttttgg attttcaatc
     8101 cgtttgtgtg aaatttgtga tacgtgaaca agtccatcga tacctggcaa tacttcaaca
     8161 aatgcaccga agtcagtcaa acgtttaact gttccttcta ctacatcacc tttagccaat
     8221 ttttgctcaa cgccatccca tggtcctggt actgttgctt taagtgaaag tgatacacgt
     8281 ccttcttctt cgttaagatc aaggattttc acttcaattt cttcaccaac agttacaact
     8341 gattttggtg atacgttacg ttcatgtgac aattcagtca agtgaaccaa tccgtcaaca
     8401 ccaccaaggt cgatgaaagc gccgaagctt gtgatacgag caactttacc agttacaaca
     8461 tcaccaacag ccaatttacc gaatacttca gcgcgagctg ctgcagtagc tgcttcaaca
     8521 acttcacgac gtgaaaggat gaagcggttt tctttagcgt taacttcttt gattttagta
     8581 tcaaattctt gacctacaaa acgctcagcg ttacgtacga aacgagtatc caacattgaa
     8641 gctgggataa atccacgaac accttcaaat tctactgaaa gtccaccttt aacggcacgc
     8701 gttcctttaa cagtaacaac ttcttcttcg cgaccaacaa gtttgtccca tgctttgcga
     8761 gcttcaaggc gttttttaga tacaaggtat gtaactgtat cagtatcttt accaactact
     8821 tgacgaagta caagaacatc caatacttct cctactttaa caaagtcatt gatatctgca
     8881 tcacgatcgt ttgtcaattc gcgaagagtc aagacacctt caacaccagt tccagagatt
     8941 gcaacgttag cttgagtcgc atcaactgtc aatacttcag cactaacaac atcaccagtc
     9001 tcaacttgac taacgctatt tagcaaatct tcaaattcgt tcatctaaaa aatcctccaa
     9061 caatcaagtt tttcttaaac ttgacatact tataattttt ttcctaagca cccgcaagga
     9121 catgttcata tcattcacgt ctttcaatta taaaaataaa ataccctaag atattctgct
     9181 ttttatcttg attattacct aagaactggg gtagctggat tcgaaccaac gcatgaggga
     9241 gtcaaagtcc cttgccttac cgcttggcta taccccatta atgaaatgga gagagaggga
     9301 ttcgaacccc cgaacccgaa ggagcggatt tacagtccgc cgcgtttagc ctcttcgcta
     9361 tctctcctac aatcaacatg gactattata tcatgaaaat ttcaaaaaaa caagacttat
     9421 tttgctaaat tataattttc aatcaaaatt tccacagctt tttccaattt tttataaaaa
     9481 ctatcgacat tcccattctt tataatggca aattgtccgt ctaattcggc aatttctccg
     9541 ataacttttt taccgatagt caatgtataa ccttcaaaac tgtctttacc aacattaact
     9601 ttggcatccg ctacttgaat ttcgattttt ttatctttct tgctcatttc atacctctct
     9661 tcactttttc tattttacaa gaaaatccta aaactagcaa gaaaaaactc tatatcaggc
     9721 ttctctgata tagagtttta tgtaaacaac ctcaattgat ttagcatagt taatagttag
     9781 taaacaagca taattgacta tcctttttag gttatcagtg agtctcaccg cctactaact
     9841 gcaaatcctt atctttgtag tctacaatag cttcaatagc tacctctatg cctcttacaa
     9901 tatcacatag actcattcct gcagtattcg gcttatttac tacttgttcc atcatatatg
     9961 gaatatgcat gaaacctgct ctcatattag ggaatttttt atccactaaa taaagagctt
    10021 ggtacatcaa atgattgcaa acaaaggttc ctgcactatt ggatacaact gccggaagtc
    10081 cctgtttttt gatagcttgt accatcgctt tgataggtaa actactaaaa taggccgatg
    10141 ctccatcaat acgaatcggt gtatcaattg gttgattgcc ttcgttatca ggtatgcgag
    10201 catcatcttg attaatagcc actcgttcag gtgttaagcc ggtcctgccg cctgcttgtc
    10261 caatacaaag tacagcatct ggttgatatc gtaatatttc tgcctctaaa acttctgacg
    10321 acttataaaa aaccgttgga atttctaccc agcgaacttc agccccatta atctcagatg
    10381 gtaataattt tacagcctcc aaagctggat taatcttttc acctccaaaa ggattaaaac
    10441 ctgtaaccaa tattttcatt ttattttcct ttactaaaat gcgagaaagt acattaagaa
    10501 tatgtgaata acaatcatta ctagagcaac acctgcttga gcctttataa cgccattctg
    10561 atctttcata tccatcaatg ctgctggtag agcgttaaaa ttagcagcca ttggggtcaa
    10621 taaggtccca caataacctg ctgtcatggc aagagcacca gccacaattg gattagctcc
    10681 cagagcaaat acaaagggaa ctccaacacc tgctgtaata acggtgaatg ctgcaaaagc
    10741 atttcccata atcattgtga atagaaccat tccaagaaca taagccaaaa ctcctataaa
    10801 gcgactatct gaaggaacaa taccgctaat cagatgagag ataacatcac caacacctgc
    10861 tacagtaaaa atagccccca aagcccctaa taattgagga acaatcccac ttgttgaaac
    10921 ttgctgagtc attcgattat tttctgataa cagactctta gggtgactat tggtaatcac
    10981 aagaacagaa attgtagcaa acaaggcggc aaggctaatc gaaatcttgc taaattctgg
    11041 aatcatttgc gctaagacca acgcaagtat tgccatcagc ataactggaa taaaaatttt
    11101 atttttcaac ctgttagatt caatattggc tttcatttca tctaaggatg gcaaggttcc
    11161 gatacggact tgcttaaaca atgttaacag cgataatagg attacaataa taccaatact
    11221 catatttggc atataggaac cacctataaa cgtaatagac aatagagtcc aaaatgcaga
    11281 tgtcccaagt cgaactgggt ttgttttatc tttataacta caataggctg tatggagaaa
    11341 ttgacaacca atcacaatat aggtcaactc taatagttgc tttgccaact ctgtcatttt
    11401 tgttctcctc ccctagtctt ttttgatatc aattttttat caaataaata attgtaaatc
    11461 cccactacaa taagtgttat aacagcaaca ataatagatg tagaagcaat ccctgcataa
    11521 ttgctttcat agcctaactg atctaatgtt ccccctatca agaggactcc cccagcacct
    11581 acaaacgtat tttgagcaaa gaaatttcca aaattttcat tcgcagccgc acgcgctttt
    11641 attgtctcat cttcaacctc tgttaacttt ctacctaatt gagactctgc agctgcttct
    11701 cccataggtt gaaccaaagg tctgacaaac tgagggtgtc ctcctagacg aattgaaaag
    11761 aaaccagcta actctcgaat aaagaaataa actgtataga agtttccaac tgtcagacct
    11821 ttaatctttc gaatcaaatc gattgatcgt tgcttgagtc caaaggtttc tgacagcccc
    11881 acaagaggca aggtaaccat aaaaatcgtg agcactcgct gattgctaaa ttcttttccc
    11941 aaaatctcca aaaattcaac gagagaaaca cctgaaacta aagctgtaac caaaccagct
    12001 aagactactg ttgcaattgt atcaaatttt aaaataaaac ccacaacaat gattgctatt
    12061 cctattaatc taatccactc catatcaaac tcctttatat tcaaaatgac agtattttaa
    12121 aaattttatc aagatcaata ccattcctta tttaatgtat ttttctagtt ctttttggta
    12181 tttgctattg gattccaatt tttctttttg ccatttttta aaaacctcgt tatattcttt
    12241 tgttgtaaca atatcttttt gcaatttcat tcctttaaag atatatggtt cccccttaat
    12301 accaacttgt gagtatggtt ttgagaatgg tactacgtta cttacaactg gaaaaccacc
    12361 agatgaagct gttggcatca ataatgaact atctgtcgac caagcttgag ctttggcata
    12421 tttttcatat ctcttctcta ggtcagtggt ctcagaaaca gcatcttcta acaatttctt
    12481 atatttatcc aaaccaggtt tagctacaac atccttatct tttcctttcg taatgccaag
    12541 gtgtttcatg tcagaaccag attttggatc cataatattc aagtaagacg ctggatcttg
    12601 atagcttgga gcccatcctg tactgttcaa atcatagtct ttttgagaag gagcaacatt
    12661 gccgtattta tcattttcca tcaaaccatc aataacattt ccaataacgt ctgtcctcga
    12721 tgttcgagtc gctatactgt agcccaatga tgctggatct actgcataga cataagaaaa
    12781 tgttgtcggt gcatctgctt ctttatcagt ttttccacaa gccactaaaa tagctgacgt
    12841 gctcaggacc actcctgctg ttaagagcca ctttttctat ttcataaaaa atctcctttg
    12901 gtttatttta atctactttt acaatccaac cttctggcgc ttcaatatcg ccaaactgaa
    12961 tacccgtcaa ttcattatat aatttacgcg tcacaggacc tacttctgtt tcactataga
    13021 atacatggaa atcatcacca tgttgaatac ctccaattgg agaaataacc gctgctgtac
    13081 cacaggcacc tgcctctaca aaacggtcaa gattatcaat tggaacatca ccctcaatag
    13141 gagttaatcc caagcgatgt tctgccaaat aaagcaagga atacttggta atagatggca
    13201 agatagatgg actcaatggt gttacaaatt cattatcagc tgtaattcca aagaagttag
    13261 ctgatccgac ttcttcaatc tttgtatgag ttgatgggtc cagatagata acatctgaga
    13321 aatgacgtga cttggccatt tttcctggta agagacttgc agcatagttt ccaccaacct
    13381 tagccgcacc tgtaccattt ggtgctgcac ggtcgtactc atcctgaatc aagaagttgg
    13441 ttgggaccaa accaccttta aagtaatttc caactggcat agcaaagatg gtgaaaatgt
    13501 actcttctgc cggttttacc ccgataatat ctccgacacc aatcaaaaga gggcgaagat
    13561 ataaggttcc acctgttccg tatggtggta cgtattcttc attcgcacgg acaactgctt
    13621 tacaagcttc tacaaacatg tctgtcggaa cttgtggcat caagagacgg tcacatgtac
    13681 gttgcagacg tttagcattt tcatcaggac ggaacagttg aacactgcca tccttagtac
    13741 gataagcttt caaaccttca aatgcttgtt gtccatagtg aagacttgga gaagactctg
    13801 aaatatgcaa agttgcatcc tctgtaagct ctccttgatc ccattgtcca tttttgaaat
    13861 gagcaagata gcgataaggt aatttcatat aggaaaaacc gaggttttcc caatcaatcg
    13921 ttactgtcat attccactcc ttattctaaa aacctatttc ttatattcta cactattttt
    13981 ctaaaatagc aagtatattt tgtaattttc agaaaatttc tccaataaaa accagctctt
    14041 agaactgatt cttcatttca cttatttatc ttcagtaact acttcctgaa gataagcgtc
    14101 aaaaacttct tcatctgaaa tcgtgtcaga aatgaagctt ccattgctag tgcgttctga
    14161 caagttcaag tcttgcaatc ggctttcata gattgttcct ttattggatt ggacaagcag
    14221 agtttggtcg ttcacatcca cttccgtact gaagaaatcg ccaacaaatc cttgctctgc
    14281 aactgctcct gccaagaaga cacgatgcgg tttgtttttc aactcacgca agacttgtaa
    14341 tcctcgtttg gcacggctgg ttgctagaat ttcctcaata gaaacacgtt tcaagcttcc
    14401 acgctgggtc aagaggtaga aggacgaagt attacagata aagccagatt ggaggacatc
    14461 atcttctttc aaattcatag ccttgacacc tgctgcctta gcaccgacaa ccggaacctc
    14521 ttcgatattg aaacgcaggg cataaccatt ttgactaacc aagacaacat catctagttt
    14581 aatcggagcc actgctacaa tctgatctgt atcgtctttg agcttagcat acttgacaga
    14641 cttagatcta taggtccgcc atggagtgaa ttcttttcgc tctacccgtt tgatttgacc
    14701 aaggcgagtc actgcaaagt aggttgtcgc atcgtcaaac tgatccagta cttccacata
    14761 aaggatttct tcattcgttt caaagtttgt gatggtttgg ctcagatgct ctccgatgtc
    14821 cttccaacga atatctgcca actcatggat tggtctgtag atgacatttc caagacttgt
    14881 gaacatcaag aggtgctggg ttgtcttggc agattgaaca aaaatcaaac ggtcatcatc
    14941 acgcttgcca atttcttcca aggtggaagc cgcaaaggaa cgtggactgg tacgcttgat
    15001 gtaacctgcc ttggtcacgc tgacgtaggt atcttcctca gcgataagac tagctgtatc
    15061 aatctcaatt gctttcgcag tgtcttctaa agaactcaaa cgaggagttg caaatttctt
    15121 cttgacctca cgaagttctt tcttcatgag attgtacata gtcctttcat caccgataat
    15181 agccgccagc atagcaatct tctcacgaag ctctgcttct tcttcctgca agacaaccac
    15241 atcggtattg gtcaaacggt acagttgcaa agttacgata gcctcagcct gttcttccgt
    15301 aaaatcatag ctaactttga ggttttcctt ggcgtccgcc ttattctcag aagcacggat
    15361 aagagcaatg acttcatcca aaatcgaaat cacacgaatc aaaccttcga cgatatggag
    15421 acgtttctca gccttttctt tgtcaaagcg tgaacgcgcc aaaatcactt ctcgacggtg
    15481 agcgatgtag ctagacagga ttggaacaat cccaacctga cgaggtgtga aattgtcaat
    15541 cgccaccata ttaaagttgt agttgatttg taggtcggtg tacttaaata agtagttgag
    15601 aacaagctca gtattagcgt ctttcttaag ttcgatagcg atacgaagac catcacggtc
    15661 agactcatca cgaacctcag caatcccagc taccttgtta ttaacacgaa catcatcgat
    15721 tttcttgact agattggcct tattgatttc ataaggaatc tcagtaataa cgatttgttc
    15781 cttaccacct tttagctttt caatttcagt cttggaacga acaaccacgc gccctttccc
    15841 agtctcataa gctttcttga tttcatcacg accctgaata atagcccctg tagggaagtc
    15901 tggtccaggc aagaattcca tgagtttatc aatctttgca gttgggtggt caatcatgta
    15961 aactgcagca tctatgacct cagctaaatt atggggagga atgtctgtgg cataaccagc
    16021 cgaaatccca gtcgaaccat tgaccaagag gtttggaaag gctgctggca agaccgttgg
    16081 ttctttctcc gtatcgtcaa agttccatgc aaaaggaact gtatttttct cgatatcctg
    16141 aagaaggtaa cctgcaattt cagacaaacg tgcctcagta taacgcatag ccgcaggagg
    16201 atctccgtcc atagaaccgt tattaccgtg catttcaact agaatctcac gatttttcca
    16261 gttctgtgac atacgaacca tggcatcata gatagaagaa tccccgtgtg ggtggaaatt
    16321 ccccatgatg ttcccgactg acttggccga cttacggtag ctcttgtcaa aagtattgct
    16381 atccttattc atagaataaa gaatacggcg ctgaaccggc ttcaacccat cacgaatatc
    16441 tggcaaagcc cggtcttgaa taatgtactt ggagtagcga ccaaagcgct ctcccatgat
    16501 gtcctccagg gacatgtttt gaatgttaga cataagatac aaagcccata aaataccaag
    16561 tgaaaataga aaattcttga agtaagcaaa ctcacaagag aatttatctt tttcacacag
    16621 tatctagggc gtgttcaact cctttcaaag aatgtagagt aggtttttat gcagtaaaag
    16681 atattttacg ggaattcctc ccgtgttcag ttacgataag taaccaaact atcctgtttg
    16741 tatttttcaa tatgaaaatc tggttttcca aaattagtct tagtttgtgt cttagccgct
    16801 cccttaagcg cctctttgag ataagcactc atagcagatt cttcattaat aatcctgcaa
    16861 ttttttcaaa ccaagatttt caaactgctt tttcacatag tcattcacat ccgactctaa
    16921 tttccagttt actaacatat tattttcttt cattaaaaca ctgtcgcttc ttctagcgta
    16981 aacttgacat tatcttcaat ccatttacgg cgtggttcta ccttatctcc catgagaaca
    17041 ttgacgcggc gttcggcgcg cgctaaatct tcaattgtga cacggatgag ggtacgtgtt
    17101 tctgggttca tggttgtttc ccagagctgg tccgcattca tctcaccaag tcctttgtat
    17161 cgttggaggg tagcgccttt accgaactgt ttacggagtt cttctagttc tccgtccgtc
    17221 caagcgtagg ccacttcttc tttctttcct ttacctttgg acatcttgta aagaggtggg
    17281 agggcaatat agacatgacc tgcctcgact agcggacgca tgtaacggta gaaaaatgtc
    17341 aagagcaagg tctggatatg ggcaccgtcg gtatccgcat cggtcatgat aatgatctta
    17401 tcatagttgg catcttcaat agagaagtct gctccaacac ccgcaccaat ggtataaatc
    17461 atggtattga tctcttcatt tttgaggata tccgccatct tggccttggc tgtattgata
    17521 accttaccac gaagaggtag aatagcctgg aacttgcggt cacgaccttg tttggcagaa
    17581 ccaccggcag agtccccctc aactagatag agttcattct tagcaggatt cttagattgg
    17641 gctggggtca atttcccaga caacaagccc ttatctttct tgtttttctt cccatttcgg
    17701 ctctcatcac gcgccttacg tgctgcttca cgagcatcac gggccttgat agccttgcgg
    17761 atgaggttag aagctaattc cccattttcc ataaggaaaa aggtcaactt atcagccact
    17821 attccatcca caactgggcg agctaggggg cttcctagtt tatccttggt ctgtccttca
    17881 aactgcaagt gttcttcagg aactaagata gaaagaacgg ccgctagtcc ctcacgatag
    17941 tctgaacctt caaggttttt atctttttcc ttgagaagac ctgttttacg tgcatagtca
    18001 ttcatgacct tggtaatggc agacttgagt cctgtctcgt gcgttccacc gtccttggtg
    18061 cgaacgttat tgacaaagga tagaatgtta tctgagaatc cgtcattgta ctggagggct
    18121 acttccactt gaaaaccatt gtcttcccct tcaaagtaaa gaactggcgt caagatttcc
    18181 ttatcttcgt tgagataaga aacaaaatct tgtactccat tctcatagtg gaactcaatc
    18241 gcttcatctg ttcgcttgtc cgttaaagac aaggtcacat ttttcaagag aaaggctgat
    18301 tcattaaggc gctctgaaat ggtattgtac ttgaaatctg tcgtagaaaa tatagtcgcg
    18361 tcaggcataa aagtaacttt ggtgcctgtt ttagacttgg gtgctgtacc gattttcttc
    18421 aaagtcgtga caggttttcc accattttcg aaacgttgct tgtaaactgc gccatcacgg
    18481 gtaatttcaa cttctaacca gctagaaagg gcgttaacaa cggaagaacc cactccgtga
    18541 agtccacctg atgtcttata gccaccttga ccgaatttcc ctccggcatg aagaatggta
    18601 aagataacct caacagttgg aattcccata gcgtgcatac ctgtcggcat cccacgtcca
    18661 tggtcttgaa ccgttagact accgtcttta ttgatggtta catcgatacg atcaccaaac
    18721 ccagacaagg cttcatcgac tgcattatca acgatttccc aaactaggtg atgaagacca
    18781 gcgccatcgg tcgatccaat atacatccct ggacgttttc ggaccgcatc caacccttct
    18841 agcacctgaa tagcatcatc attataattg ttaatattga tttccttttt tgacacaagg
    18901 aacctcctat tcgttcatct ttactattct acaggttttc caaggatttt gcaaaatttt
    18961 tctttctccg atgtgacaat tttagcagag attctctgct tttctttccc aattcatgat
    19021 ataataggag tatgattaca atagttttat taatcctagc ctatctgctg ggttcgattc
    19081 catctggtct ctggattgga caagtattct ttcaaatcaa tctacgcgag catggttctg
    19141 gtaacactgg aacgaccaat accttccgca ttttaggtaa gaaagctggt atggcaacct
    19201 ttgtgattga ctttttcaaa ggaaccctag caacgctgct tccgattatt tttcatctac
    19261 aaggcgtttc tcctctcatc tttggacttt tggctgttat cggccatacc ttccctatct
    19321 ttgcaggatt taaaggtggt aaggctgtcg caaccagtgc tggagtaatt ttcggatttg
    19381 cgcctatctt ctgtctctac cttgcgatta tcttctttgg agctctctat cttggcagta
    19441 tgatttcact gtctagtgtc acagcatcga tcgcggctgt tatcggggtt ctgctctttc
    19501 cactttttgg ttttatcctg agtaactatg actctctctt catcgctatt atcttagcac
    19561 ttgctagttt gattatcatt cgtcataagg acaatatagc tcgtatcaaa aataaaactg
    19621 aaaatttggt cccttgggga ttgaacctaa cccatcaaga tcctaaaaaa taaaatgcca
    19681 gttctgtgaa ctgcacccca aaagttagac aattaattta tccgaaggat ttagttctgt
    19741 attgcacagg gctaagtcct
//
LOCUS       DAVBCG010000024        19548 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBCG010000024 DAVBCG010000000
VERSION     DAVBCG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19548
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.024"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..777)
                     /locus_tag="VQ495_000725"
     CDS             complement(<1..777)
                     /locus_tag="VQ495_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2530711.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVY"
     gene            complement(873..2183)
                     /locus_tag="VQ495_000726"
     CDS             complement(873..2183)
                     /locus_tag="VQ495_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2530712.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(2310..3305)
                     /locus_tag="VQ495_000727"
     CDS             complement(2310..3305)
                     /locus_tag="VQ495_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2530713.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            complement(3667..5148)
                     /locus_tag="VQ495_000728"
     CDS             complement(3667..5148)
                     /locus_tag="VQ495_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2530714.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            5591..5755
                     /locus_tag="VQ495_000729"
     CDS             5591..5755
                     /locus_tag="VQ495_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2530715.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            5839..6405
                     /gene="amaP"
                     /locus_tag="VQ495_000730"
     CDS             5839..6405
                     /gene="amaP"
                     /locus_tag="VQ495_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2530716.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            6417..6587
                     /locus_tag="VQ495_000731"
     CDS             6417..6587
                     /locus_tag="VQ495_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2530717.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            6618..7226
                     /locus_tag="VQ495_000732"
     CDS             6618..7226
                     /locus_tag="VQ495_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2530718.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            7257..7460
                     /locus_tag="VQ495_000733"
     CDS             7257..7460
                     /locus_tag="VQ495_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2530719.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7570..7848)
                     /locus_tag="VQ495_000734"
                     /pseudo
     CDS             complement(7570..7848)
                     /locus_tag="VQ495_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8046..8333
                     /locus_tag="VQ495_000735"
     CDS             8046..8333
                     /locus_tag="VQ495_000735"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2530720.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(8611..9381)
                     /locus_tag="VQ495_000736"
     CDS             complement(8611..9381)
                     /locus_tag="VQ495_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2530721.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(9396..10244)
                     /locus_tag="VQ495_000737"
     CDS             complement(9396..10244)
                     /locus_tag="VQ495_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2530722.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(10241..11716)
                     /locus_tag="VQ495_000738"
     CDS             complement(10241..11716)
                     /locus_tag="VQ495_000738"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2530723.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(12039..13394)
                     /locus_tag="VQ495_000739"
     CDS             complement(12039..13394)
                     /locus_tag="VQ495_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2530724.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(13404..13721)
                     /locus_tag="VQ495_000740"
     CDS             complement(13404..13721)
                     /locus_tag="VQ495_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2530725.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(13738..14055)
                     /locus_tag="VQ495_000741"
     CDS             complement(13738..14055)
                     /locus_tag="VQ495_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2530726.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(14068..15753)
                     /locus_tag="VQ495_000742"
     CDS             complement(14068..15753)
                     /locus_tag="VQ495_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2530727.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(15758..16654)
                     /locus_tag="VQ495_000743"
     CDS             complement(15758..16654)
                     /locus_tag="VQ495_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2530728.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(16657..17919)
                     /locus_tag="VQ495_000744"
     CDS             complement(16657..17919)
                     /locus_tag="VQ495_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2530729.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDILLYLPFVIAIEKRFKLLED"
     gene            18199..19377
                     /locus_tag="VQ495_000745"
     CDS             18199..19377
                     /locus_tag="VQ495_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2530730.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALFYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT         6655 a         3777 c         2826 g         6290 t
ORIGIN      
        1 gtacaccata gtaattgttg agtttaatgg gccaccttgc gtcataacca ttggttgtac
       61 aatcagctta aaagcagaaa ttaaagttgt taacaataca aacagggctg ttggttttag
      121 taggggcatc gtgatataac gaaactgagc ccattttgag aatccatcta attcagcagc
      181 ttcatagaca tcttgtggaa tattctgcat tcctcctaaa aataaaagca tctgataacc
      241 tgcaccctgc caagcagaaa caaaaacaat tgcatacata gcttgtttag gactagtcaa
      301 gaaaggttga gaagcaatac ctaccttatt taaaatagca tttaataagc cactatttgg
      361 atttaaaaga taaagccaaa gaatagaaat aactaccaag gacatgacaa ctggagcaaa
      421 aaaagccact ttaaaaaaca tatttccttt tcgcttttta ttgacaatca aagccattcc
      481 taaggccgct cccaattgaa ctggaataat ccaaacaaca aatttcaaag tattcaagaa
      541 gctctttaag aaaatcggat cttttgttaa tcgaataaaa ttttcaagtc caacaaattt
      601 tctttcatct ggtgtcaaca aataataatc tgtaaaagcg taataaatga ccattccaac
      661 tggaattact aaaaagatac ctaataaaat taaagctgga gctaaaaaac tataacctaa
      721 tatgttatcc tttatttttt cacgtctttt tgtatacaca acagtttctt tactcatatc
      781 catctccttt aatcatatat atcaaactag gctattaaca agaaagggga aggtagttcc
      841 aaacattttc cttccacctt cttttttaca gattatttga gtgactgatc aatagcagtt
      901 tgcatttctt ttgtacgagt atccaagact ttttgcacat ttggattttc ttcataatag
      961 ctaatatctt gcattgcttg ttgaaaagct cgagaaacct gaggatatgc tacgacaact
     1021 gggcgagcat gagctgtttt tgaattttgt tccatcaaga aacgcattgg ttcggatact
     1081 ttatctttga tattttcaat tgtagattta cgaattggaa ggaccgaatt ccccaagcta
     1141 ataatttcac ttgattctgt atttgtagca aatttaacaa attcagatgc ggcctctttt
     1201 ttatcagatt ttgtcgtcac agctaattgc caactacctg aaggggatac taattttttc
     1261 gtcttactag atactgggta aggaaggata ccaaaatcaa tatctttgta gtttgtattc
     1321 atgtcagcaa tcgtccaaga accactcaaa agcattggat actctcctgt ttcaaaacct
     1381 ttttcgactg gactaacagt tgtatagcct tcttttacaa gattttgaat gaattgaact
     1441 gcttctacac tctctttgga gttgaagtat ccttctgcct ttgttccatc ttcgtttacg
     1501 acagaaccat tatttgacca aatcaacggc atataagcat atggcaacat ttcatcgttt
     1561 gaattaatac gaaaatcaat tgctggtttg ttataatgat ctttcaattt tttagcaatc
     1621 gtattaaatt cgtcccatgt ccatggcttt tctagcgttg gaagctctga ttccgcaatt
     1681 ccagcctctt tgaacatttt tttattgtaa taaacaccaa cattcgattc agaaaatcca
     1741 aatgcataga atttcccatt ataagttccc tgttgtttaa tactgtccaa aacatcatcc
     1801 atattattat cttttagata atcatctagc ggagtaatta ctttagattt tgcataagct
     1861 gccgtatttg gtccatcaag ggtaataaca tctggaagac tattagtcgt aatagcagca
     1921 ttaaccttgt cttcatatcc tccaccacta ccactacgag gaatatactc agttacaacc
     1981 ttgtacttac tttttcctga ttgattaaag ttatcaacaa ctttttgcca agcttttcct
     2041 tcatttgttt cgtcagagaa ttgtacccag actttaatat catttgaaga actcgcttgt
     2101 tttgagttag ttcctccact acaacctgca agtatagcta acgatagccc cgcaacaaca
     2161 gataacattg tacctttacg catcattttt ctcctttttg aaacgtttca ttttttattt
     2221 attttttaaa caagcttcta gcctattttt gaaacgtttc atttgttgat tatattataa
     2281 cagcgctttc attaattgtc aagcgctttt taaatttttt ttgttgtttt atctccccca
     2341 aaatatgtgg gaattacgat tcgtttttct agaatctctt ctcgcttaat cattttcagt
     2401 atcattgcaa cagattcttt tgccatcata ccaatatctt gaactattgt agacactaga
     2461 taagattgat cgacagaagt ttttaaacca tcaaacccaa ttatctggta atcatcaggt
     2521 gctattttcc ccagttcttt cataacacta ataactctca gagccatcat atcatttatt
     2581 gtaaatatac catcaatttc tttatgagat tgcaaaaaaa ttcttatctg atcttcagga
     2641 tttattaaag gttctttcat ttcaaaatta tagacatcta ctcctaattc cttagctttt
     2701 tctctgaatc ctttacttct gagtatagtc tcattctcat acatattaat tgaaccaata
     2761 aaagcaaggt gcttattatc ccttctaacc agttcctctg ctgcagtttg tcctcctttg
     2821 taatttgctg aagtaacata gcatacattt tccgaaaaat gtctatctat acttacaaac
     2881 ggtaaatcag ataaaatata tttgtcaata tcagaatatg taataccaat aattccatcc
     2941 actttatttt gtttcaacat ttcgatatat tcgatttcat tttgtgaatc gctatcagcg
     3001 ttacaaataa ataatttata attatatttt aataactctt tctcaacatg ataggcaaac
     3061 tccgaaaaaa atggatgcca tatgctcgga ataatcaaag cgatagtttc tgttctattc
     3121 tttttcatac ctcgagcata attatcaggg atatagtcca atgtttttat agccctttga
     3181 actttcctta aagttacttc tttaatgcct ttttctttat taattacacg tgaaacagtt
     3241 ccaacactaa ctcctgcttc taaagcaaca tctttcatgg taattgattt tctttgttct
     3301 accatattat cacctccttt caatatatag tatcatgcaa atgcttttta agcaactatt
     3361 tctcaatcat ttttggccag atcatttatc ccatcatgaa taaaatcact ccaattagct
     3421 tttgaaaata cttcaatttt catgtgtaaa catctacata aaacaggaaa agccttggtt
     3481 ttatggcttt tttcgtatct tctacaaaaa aagcaagagt tttagagggc tataaatcta
     3541 gatgtacatt ttgcttaaat gattgaaggt cttttcttaa caaaaacacc ccaaaaatta
     3601 gattttttct gtctaacttt tgaggtacag ttcaaacgcg aaatagcgtt tttttgttat
     3661 ttttggttac tcatctaatc gaataaacat catggcattt aacaaatata tgagtgagac
     3721 cgtgtttata ttatttgaat agatgagtct cttattttca ataggaggaa taataaaatt
     3781 agaaataatg atatcataag atgaatcttc taaagattcc tttgataatt ctaattcagt
     3841 ccaaacttcc agttcaaaat tattgctaca gtaataagaa agtgtctctg caacgaattt
     3901 tgcatgatac tgatcaaaat tactcataac caaaaccttt agtttaggct gattttgtag
     3961 caaattaatc accaaatgtt tggtatgagt gatgaaggta taagatagat gatttaccat
     4021 cattgaacta gaacaaacct caagagtctc taaatagtga gaaagctctt tttttatatc
     4081 tgaaacaaat tttggaaaaa tattttgaaa gttcctgatt gtattccctt tttgatcaaa
     4141 taaaataaac tcagtaaaca actcttgacg atacagatgt gcggtattat gcagatgcca
     4201 aatcagatta tccttattct ccatttcaat ctgatacttg actgaaatct gatcaataaa
     4261 atcactcaat agatggtaag atttttcaac ataactatcc ttttttacgc atttcataaa
     4321 gagactttca tctatgaaaa acattttttg aaagtaagac acaaataatt ggcaaacaac
     4381 ttcttcatct aaagagatat tgtattctga ttcaaaactc tgagcaacac cttctattcc
     4441 ttctgcctgc attaaaaaat ccaaactttg gtcgttaaaa gaatctttat ctacttccat
     4501 aaaatgacca aactttattc tatataggtt cgtaactagg agcaacttta gcattctatg
     4561 cgttgacaaa ttcattggaa agcttgtttc cttataaacc aattctaaca attgagatag
     4621 tggctctgat gaaaaatttt caaatggcca ttctaggaaa taatattttt ctgaaaaata
     4681 ttgtgcaaaa aagtaacgaa tgtctctctc atttccaatg atttgaacag gggtcagact
     4741 gacttcaaat tgaaattgcc ttttaatcac tttattgatt tggctaataa tacgatagag
     4801 cgaagatgaa ctgatataaa attctttaca aatactctca gcttgacaac cttcattaaa
     4861 gaagatgaat tctaaaatcg aaaaatgagt tgaatgttta aagaaatgat ggtaaaccat
     4921 ttcaatatca ctatcatcgg tattaataat gcgtatacca ttagtagaag aatgaaaaat
     4981 caagtcagga aaagcagatt taacatggga tagatcatct ttgactgcac gttctgtaca
     5041 atttaataac tctgctagtt cagaacgatg aaaccaacgt ttatgttcaa ataataattc
     5101 taataattct aattgcctat gacttttttt agataataaa tctctcatga atatctttct
     5161 ctctttataa attatcggat taaacctctt gcaattatac cacaaagaat aggtatagca
     5221 tgatataacg acttttccta aaatctttta tttcgtataa taacactacg gagacaatat
     5281 ataaacaatt ttcttatttt accgtctatt gagggcgtga atacagaatc aaattcaagt
     5341 ctaaagatta tatttttaat tttaaaaatt atataatagc aacaattaaa gaatttgatt
     5401 ttttaaaatt atataataat aacaatcgaa ataattgact tttctatatt aaagttatat
     5461 aatagtatta atcaaagaaa ttgatttttt gatattaaaa taaaaaagga gggtaggcag
     5521 tgttgtgatc aattattgct ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa
     5581 aagtagttct atgggaatca tcgcaaatgt attcgctggt ttagtcgggg catatgcagg
     5641 acaatctctt ttaggtagtt ggggtccatc aatcgctgga atggctttgc tcccatctat
     5701 tgtaggtgca gcgattgtga ttactgtagt gtcattcttt acaggtagaa agtaaacttt
     5761 tcgccagtaa agttagcaaa ctatttttaa atcaatgacg ggaaaaatag tttaaatgtt
     5821 aaatcgaaag gattgtatat gtcaaaagca aagaaaatat gtttcattat tttctgtatt
     5881 ttaatcttga caattttcct tcctgttttg atagattatc atcaagttag tgatctaggt
     5941 attcatctac ttagctggag acagaactcc gtagttgaat tctatcttgc tagatatgtc
     6001 ttttggggga cagtggttct atcaacttta gttttattat ccattttagt tgtgatgttt
     6061 tatcctaaac gttacttgga aatccaactt gaaactaaaa acgatacatt aaaattaaag
     6121 aattcggcaa tcgaaggttt tgttagaagt ttggtgagtg atcatagatt gatcaagaac
     6181 ccaactgttc atgtaaattt acgaaaaaat aaatgtttcg ttcatgtaga aggtaaaatt
     6241 cttccttcag acaacatcgc tgacagatgc caaataattc aaaatgaaat aactaatgga
     6301 ttgaagcagt tttttggtat tgagcgtcaa gtaaaacttg aagttgcagt aaaaaattac
     6361 caaccaaaac ctcaaaacaa aaagactgtt agtcgtgtga agtaaggaag taaaaaatgg
     6421 aatggcttaa acaatatcga tatccaatta tcgctggtct cataggcgta tttctggctt
     6481 gtttgattgt ctcctttggc ttcttcaaaa caatatttgt attgatttta ggagcactgg
     6541 gagttgcagc tggattatat atcgaaaaaa actatataga taaataaaaa ttactaattt
     6601 aattaaagga gtttcatatg tcaaacgaaa aaaacacaaa tactaacgta gaaaagaaag
     6661 atgctactgt tgtagctcac gaaatcaaag gggaacttac ttacgaagat aaagttatcc
     6721 aaaaaatcat tggtctttca ctagaaaacg tttcaggtct tttgggaatc gatggtggtt
     6781 tcttctcaaa tcttaaagaa aaaatcgtta acagcgatga cgtaacaagt ggtgttaacg
     6841 tagaagttgg taaaacacaa gttgcagttg acttaaacgt tattgttgag taccaaaaaa
     6901 atgttccagc tttatattca gaaatcagag aaatcgtatc ttcagaagtt gctaaaatga
     6961 ctgacttgga aattgttgaa atcaacgtaa acgttgtcga catcaaaact aaagaacagc
     7021 atgaagcaga ctcagtaagc cttcaagatc gcgtatctga cgttgctgaa tcaacaggag
     7081 aattcacttc agaacaattc gaaaaagcta aatctggtct tggatctggt ttctcaactg
     7141 ttcaagaaaa agttagcgaa ggtgtagaag ctgttaaagg tgcagcaaat ggtgtagtat
     7201 ctcacgaaaa cactcgtgta aactaagata aaataaatat aacaggagaa attatcatgt
     7261 cagtagaaga aaaattaaat caagctaaag gttctattaa agaaggtgtt gggaaagcca
     7321 tcggtgatga aaaaatggaa aaagaaggag cagctgaaaa agttgtttct aaagtaaaag
     7381 aagttgccga agacgctaaa gacgctgtag aaggtgctgt agaaggtgtt aaaaacatgt
     7441 tgagtggcga cgataaataa ggttaaaagt tactttatct ttttggtaac attagtcaaa
     7501 agagtctgag tcaagatgat tctcagaaaa caaaaagcta gagattccca attgcggaac
     7561 tctagctttt taattttgcc tctttctctt attatatttc agcaggttgt tggccatgag
     7621 tatgaatccc atgtcaattc tcacttgacg cttacctctc agatgacatc tcttataacc
     7681 caaacaaacc tttatctgcc caaagacaga tttcatatca atcttacgtt tagcgaaaat
     7741 ttgtctaccc ttggaagata aaagtgcctg atattcttta gtttttaaac actggtaacg
     7801 ttcattcata tacagtccct tttgaggggc tgattcaggt tcataatcgc agtcaacatt
     7861 gatttcaagg ctgtttgctt tctatctccc ctttttcatg atatattttc gtacaggttc
     7921 ttcaatagtt tctatagtaa aatgaaccaa aaatagtaca cgttgcggta taatgttctt
     7981 gtggcatatt cattagattt tcgtaaacag ttctctccta tttgtgagcg aacaagtata
     8041 gtcaattgat tgtcagtttg aagaccaaga gtcgtaaggc aaaggatatg gcagaatcca
     8101 tccaagggtg gctggcacaa tttctagtga acctctttaa atctattacg tttgactgtg
     8161 gaaaagagtt ttcaaaatgg aaagatattt ccaatcatca cgatagtgag agcttttttg
     8221 cgaatctagg atgttcaaga cagcggcgtc tcaacgaaca ttcaaataga cttttaaggt
     8281 gtcatgactt gccaaagcag acagatttca acgaagtcag tcaagaattt taggctggac
     8341 aaaaactcaa tttcaggaga aaatgaaata aatattccca caataaaacg catagtatca
     8401 aagtttttcc aacacctgat attatgcatt ttttaatttt aaagactttt tcccagcctc
     8461 aagaaatcac atcccaccta aatcctttgg ataccaaata cctcatcagg tattttctga
     8521 gttggtaaga atgtctagct taatttgaca aatggagggc gttaaatcca gtttttacag
     8581 gaagtctatt taaatatttt cacactgacc ttacaaaata tgaaataaat tatataaagt
     8641 cattcccatg acaaagaata acatcccttt aaaacagtat cttacccaat tttcaggtaa
     8701 gacttttgat acaaccgtcc ccaatacccc tccgcatata gaagcaagaa aaataactgg
     8761 aacaggtgct aaatgatact gatttaaacc tgtaaccaca acaattgtag ccaaatgaga
     8821 cagctgggaa aagaaaataa ttgcaagaga atacatcgtt gcttctttta tcgaaataga
     8881 aaaaaacacc atcaataaag atacattgag cggaccacca ccaatcccta aaaaactaga
     8941 aattaagccc aatagaagac cacaaatcat atagaatcct atcttatcaa attgataagt
     9001 ttttttaaaa tctgttaaaa caaatgatac aagtagcatc acaaatagta aaatcatttg
     9061 aacaatggat actagccgta ctgataactg agataacaaa acttgaaaaa taagatgacc
     9121 tagaaatcct cctaaaacag aagaaaagga taccgtaaaa atcaatctcc attttatctc
     9181 tttactttgt gaaaatcttt tgattgttga aataatcgcc attataaaaa cagagaaact
     9241 agaataaaaa gcaatatcac tgaccgaatg gtatccaaaa ctatccataa gtggcttaat
     9301 tataattcca ccacccatgc ctgatataga acccaatcca ctagctatta taatgattag
     9361 aaaataaata atctcaacca ctttttaagt ccatcctatt ctttcaaaca tctaaaacct
     9421 agatggctac tagtggaagt agagatacat ccatttctag cagctactcg gtatcgatta
     9481 caataagaac aatgacataa aaaggaacct cctctgatag catactcctc atcttgtatc
     9541 ccatattttg gcaactcata gtcatcttca ttaaaactag ctaataaggt atatctcgga
     9601 ttgcgacacc attcccaaac attcccaatc atttgatata gaccaaaatc attaggtaaa
     9661 aattcataga caggagccgt tcctataaat ctatctaagg cagtattttc ataaggaaat
     9721 cttccttgcc aagtattggc atgataatat ccaccttcta aaagagtatc tccccaagga
     9781 tattcagatt gtcttcctcc tctagcagca tattcccact gtgcttcagt aggtaaagac
     9841 attccgctcc aattacagaa agccaatgca tcttctaaag caacatgaac tactggatgg
     9901 tcttctaaac ccactagatt actgttctca ccgtagggat gtttccagca agcgttcgga
     9961 acttgtaacc accacggagc cccagcagga tgaggatatc cctccctttc tgcctctgga
    10021 acgaacaata taaaaacaaa agaccactct tgtcgctcag ctagtgtttt atagcctgtt
    10081 tctttcacaa attgagcaaa ttcttgattc gtaactggag tatccgcaat ggaaaaatcc
    10141 tttacactaa cagcgacttg aggaccttca aaatcatcta aaaagccctc ttcagaattt
    10201 gttcccatca taaatgtccc acccttgatt tttttcattt tcatgattga ctatcccccg
    10261 ttttcgttag agttggcgtt attttagaaa gttcaacagg aactaactct tcatccacaa
    10321 caaaaccttc ttctctatat ctaagaaagt ctactaattt tttcttcatc tgacgaacaa
    10381 taggttggta tttttcgctt ggatacaaat cattcatttc atgcggatct tttttcatat
    10441 caaataactg ataatgattt aaaacaggaa accagataaa tttccactgg tctgtcaaga
    10501 tatattgact agaatctttt cctaaagcat gttcaccatg aaattctgtt cgccacccct
    10561 cgtactgtcc aaataataaa ttcttaacac tccgtccatc tagttcatct gtagtagttc
    10621 ctcctgccaa atccacgagt gaaggaaaaa tatcttgaat tttcactaat tgcttgattg
    10681 ttcccctatt accagctatc aatccagccg gatcgtaaat aaacgaagga atgtggatac
    10741 ttccctgata aggatatccc tttctaaaca gataatgttc accaagctga tctccatgat
    10801 ccgaaacaaa ccagataatc gtatctttat catgacgaaa ttcttttagg gaagtcaaaa
    10861 agcgactaat ttgatggtct atatgagtaa tcaatccaaa ataagcagca accattcttc
    10921 tttgatcatc ttcctttaat ttccctctta aagcataaat agacggaatt tgcttttcta
    10981 atacttccca attcccgata tgcaaatcga gaaattgagg caagcgttcc atatacatat
    11041 caaaatagta ttttggggga tttaatggag catgtggttt ttcaaaagac attttgagaa
    11101 aaaaaggaac cgttggatct cttctttgta aaaatgaaat ggactcactc acaacccagt
    11161 ttgttggatg taacttctca tctttatccc aaggtctagc ttcccaagaa ttacaatcca
    11221 ttccgtcatc aatcaaatca acatcataac caactttacc ctttaaaaag gctagatagt
    11281 cactaacata atcaaattga cttccataag ccttatcata ctttctatca acatgtagat
    11341 acccatcgtg aagtaagaca tgatcaaacc caagtctctg tctggaagga aaaacatgca
    11401 tcttaccaat acactctgtt tgataaccca tatctttaaa gacttttgga agagtattag
    11461 taaaattcca aggcacctca tcttgataac caacacgtcc acttttatct tggtctaagc
    11521 cagtcaataa agctgcccta gcaggaacgc aagaaggaac tggagaatag gcattttcaa
    11581 aattataacc tacactagcc atcatatcta atgttggagt gctaactagc ttatccttac
    11641 tattcaagga taaggcgtct gctctcattt gatctacaac aatcaaaata atatttggct
    11701 gttttgtctg aaccataaaa tctcctttct aatatggcaa aagaggcaca agaagatatc
    11761 tacctttact gcaccccttt ctatatcaat ctctctatat aaagcaataa cattcttgtt
    11821 atgttttata gaacaatgaa ctaaaatatg actaaatcga ttaggaaatt caaatcattt
    11881 tctagtactg ttttagaagt tacagtgtac tattccaact tcaacaaact ataaaccttg
    11941 gtctaataac aattttagtg gagataagaa atcctacacc taactcatct tacacgtaat
    12001 ctatttctat tttatcacaa aaaacgcaag taagaccatt aactcaattc agttttatct
    12061 gccattttca caaatgggaa ataagtcaag acactaataa tcaaacaaac aactgataag
    12121 atgatggcac gccaatcaaa tgctgtagag aagaaaccat ataaaattgg aggcattacc
    12181 caagtaacat tttgtgtaac aggtgaaaca agaccccagc ttgttgccca gtaagctacc
    12241 gttgccatga aaaccgggct aagtacaaat ggtataaata gcaaaggatt caagacaact
    12301 ggtaaaccat aattgaccgg ctcaccaata ttaaacagaa ctggtgctag accaagttta
    12361 gcaacttttc gataatgact gtttcttgaa aaaattaaaa tagcaagtac caatcctaat
    12421 cctccaaacc agacaaacgc cccaaaagac ccacttgtcc atatataagg aatcggttca
    12481 cctttttgga aagcatccag attcgctaac atagcaactc caaacagccc ttccatgatg
    12541 ggagccaata catttcctcc atggagacca aaaaaccaga ataacttatt taaaaagatc
    12601 atcagaataa ctgcaaagaa actttgagac aaacctagta atggcgtttg taacaccttg
    12661 taaacccaat caatcaataa gtcattgcta agtaaatgga aaacataagt caagatggct
    12721 actatataca tcgccataaa tcctggaatg atagaagtga acggcttagc aatcgcaggg
    12781 ggaactgaat ctggtaactt gattacccag ttctttttca ttactttaca gaaaataata
    12841 gaggctaaaa atccaatcat catggctgta aagtagcctc tggcattaat atggtttcct
    12901 ggaatcacat tcccaatagt taccatcaga tttttaccat caaatgctag attatcaatt
    12961 ccttgtaaga tctgatctaa tttcacatct cctacatttg ccaaagggaa actctttgta
    13021 actgtacttc caatcgaaat gacaaacgaa gcaagtgata ccaaaccagc agaaactgta
    13081 tcaaccttgt aaatcttagc gatattcact cccaagcaat agatgaacaa caaagaaaca
    13141 attggtatac ttcccttgaa taccaaatta ttgatgtcaa caagccactg aaaggttttc
    13201 gtaatacttt ctaggtgaaa ttgttgtggt aaatccacta gaaaagcatt taataacaaa
    13261 gcaatggaac ctgtcataat aacaggcata gtccccacaa atgaatcacg taaagcaact
    13321 aaaaacttct gattcccaac tttcattgca attggagaaa gtttttcttg cattttatcc
    13381 aacattgtat tcatactaaa ctcctaattt ttttcaaatc gttcgtatac ttctaccact
    13441 tctttcgcca agtcaacaaa agtcagactc gtcattaaat gatcttgcgc gtgtaccatc
    13501 aagaggctaa ttgatgtttc ttccccctgt gcctctcttg tcaacatttc tgtttgagcc
    13561 ttatgcgcct gtaataaggc agcattcgca tcagctaatc ttcgattggc tttagagaaa
    13621 tccccttttt ttgctgcctg aatagcctcc atagcattac ttttggcttc cccaccatac
    13681 ataattagcc ccattacaga ttctaaatta ctttcatcca taacattctc ctcaatctta
    13741 gtctaataaa ctttcagcta aatctaaaac tttagaacca ttcatcattc cgtaatctgt
    13801 catcggaata accgccacag gaatctgtct atcttttagt ttttcttgaa agtcccctag
    13861 taaatagcga acttgggggc ctaaaagcaa cacatttact tcttttgttg ctacaatttc
    13921 ttctgcttca ggagctggaa ctgcaaaaat ttctgcatcc aaccccttat cttctgctgc
    13981 cttttgcatc tttgtcacta gcagacttgt actcatacct gctgcacacg ctaacataat
    14041 tgttacttta gccatagctt actcctttta ttttattgtc aatgttactg tttttcgatc
    14101 aactttagac tcataatcta aatgtttggg acgactagta taatgcaaat tataaactcc
    14161 tggaggcatt tgtgaaatct tttctttttc agttttattg cgaattaata aatctaccga
    14221 agtattccct ccaaagttat taaacatagg ttcattttca atagaataat ctaactcata
    14281 tccactatta gttgtaattc cttttaataa atcaggaaca tctcctctgt ttattactat
    14341 atccttagcg ccatttattt tgggagattg actataatac tcttcagctt gatgttgttg
    14401 taaatcattt ctagagtaag aaacataatc aactacaaat tcagtccctg ataaattatt
    14461 gtccactacc ccaggccaac ttcctcccat cgctaaattt aactgaacat actgaggtct
    14521 attaaaaatc tttttcgcta tattatctga tccataatat acttttctaa caatatgatt
    14581 gtcaaccatc catacaatat aatctttata ccaatcaatt gaaaaagtat gatacgaatc
    14641 attaaagttt ccactcgata atttaaaggc tgtaccacgt cctgcaaatt ttccatcatc
    14701 attatccgac tgtccataat gaagcgtttg ataaactgtc ttattcccac ttccatttga
    14761 atttcgttca ccaactaatt ccattatatc tatctcacct gttgacggcc atcctcgtcc
    14821 ctgaacagga ttaatcttcc catcaagtgt aaaatccgat ccaagagtcc agaaagcagg
    14881 aaaaactcct tgacctttcg gtaattttgc acgcatctct aatttaccat aaagaaattc
    14941 tactttccca tgtgttctaa cacttcctga attatataca acctttctgt gattgtttct
    15001 tgggttaaaa tactgatctt ccttagagcg attcgtagct ctaagaacta aatttcccga
    15061 acgtagaaaa acattctcat cacttcttac ataatgttgc tgttcccaac cacgaattga
    15121 tcctaattca tatccccaat attttttatt taattgttcg ccatcaaaat catctctcca
    15181 aattttctta tatgaatttc ctttagacag ttgatacatc tctacattat caataaaaat
    15241 attagatttt tttgtagtat cgtcttctga ccatttcact aattgaatca aaactttatc
    15301 gtgtttaccg gaattgaaag aaaacatgta ttttttccat tcggttgtat taactgtttg
    15361 atcaacagct tctccaatgt tattttgaac aatctctcct tgcttattat tatcaaacca
    15421 tcttgcggta aaatatccct ctgctccact aatatctact ttggcaaaag cagatactaa
    15481 ataatcttca ttaggttcta tatcaataac ttgtccaata tatccatctg ctctattatc
    15541 attaattgca gtagaaggaa tcatacctga gtaatgtccg ttactataac tctcatttga
    15601 attaattatt cccctatttc tagagttcca aaacagctct ccttgctcaa aatttgaatt
    15661 ttttattaaa ttttcattag caaaaacttt cacatgaata aatattaata aaaataatat
    15721 aaataaactt gaaattaatt tcttattctt cattttatca cctatttact gacctagcca
    15781 agcgagatac tgtatgtctc actcctctaa ttgccttgct caatgcataa atattatcga
    15841 ctgttgccag tcccccatat cctgcgtcac ctatatgttg aatatcaact ccacaaattt
    15901 tatttctaag tgcaatttcc ttgatagtat ctgtatcaga tgtttcttgg ctagtaccaa
    15961 tagcactcag tactagccca cccttactat gaacgagatc aacgacttca cgcaactctt
    16021 ggtcatgaaa ggctggaacg gttccaactg ctggaacaag tatcacatcc gcacctgctt
    16081 ccaacaattg ctctgcgaca gaaagctctg ccacaggctc attcactcct gccccgtgca
    16141 tcttacctgc aataatcaaa ccagaaaagt tttccttagc agtttgaaca gccttaatga
    16201 tttctcgatt gctaactcct gttccaggat ttccagtcaa acagacaaag tcaaaaccta
    16261 actcctctat tcgattcaat gtttcaacac tagcaacacg acctgcaaca atttcctgtg
    16321 tttcctctag catctttgca gatgggtcaa tcggttccaa atttacacca attggacaag
    16381 caacaagctg gtgtaagcga tgaataacat catcacctga actatccaaa gcataaattt
    16441 taggctcaaa aacatcaaca caattcaata aaatcatatc agctccaaaa gctctagcaa
    16501 tttcagaatt agttatatcc ccaacaaatg tctcacgagt aactacattt tcagatagaa
    16561 caactctacc ttcacttgct aaaatacttt gttttaattc ttcagcattc atctgtaata
    16621 tctcagatgg atttgcacta attaaacgtt tcattctcaa tcctccaata acttaaatcg
    16681 tttttcaata gctattacaa atggtaaata aagtaagata tccaaaacaa tcaatacaat
    16741 ttgtagtaat gaaccagaaa tacttcctgt agcaagtaaa ccacttataa caggtggcat
    16801 agtccaacct ggatctgtat aagttagcgg aaccaaacct gatgccattg ctccccacgc
    16861 tactagtaag ttaaacattg gagctaagat aaatggaact agtaacaaaa tgttcaaaac
    16921 aataggaacg ccaaacattg ctggttcatt tatattaaat aatccaggta taacagttat
    16981 aggtgccaaa gtttttagtt gtttactagc tttcttctta tgtgctaaat atcctaacgc
    17041 aagaactaaa cctatggttg caccaccgcc acctatgaat acaaaattat ccataaatac
    17101 gctagtaaaa atatttggaa gagtttctcc agtttgataa gctactttat ttgcatctag
    17161 attagctaac cataacggtt tcataactgc attaacaaca tttcctccgt gtaatcctac
    17221 aaaccaaaaa ctactattaa ggaaaattaa gataagaaga ccaataacat tatttcctaa
    17281 aagtccaagt ggacctccca aagctacttg agcaatttca tgaagattag gtaaacttaa
    17341 tttatctaaa gttgcataaa caatcaacca accaacaata gtcactgcac caggaataat
    17401 ggcactaaag cttttagata ctgctggtgg aacaccatcc ggcattttta tctgtatatt
    17461 atgatttata aaccattggt agatatagcc attaatcaat cctaaaatca tagcaacaaa
    17521 taaacctttt gatgccatga aagcggtagg cattcctgct ccagcctctc ctctaatgaa
    17581 aggcgtaact gtaataaaag atgataatga cacaatacca gcaggaatac catcaacttt
    17641 gtattgtcta gccataaagt aagcgatacc aaaactagct acaagaccaa gtaatccaaa
    17701 actactatca acacccttcc ataaatagtc agcaacacca atatcaccca gatatttttc
    17761 ccatcctgga ataggaaaac ttgcaataac cattagtaat gaacctatca acaataaagg
    17821 tatagttaga gtaataccgt ctcgaataga aaccaacgct ttattattac ccaatctagc
    17881 cgctactgga agtaactttt cttgtaataa tttttccata accttttcct cttaaatata
    17941 ttttattatc aaaagttatc aattatcttt attttttgac gtcaagttgt tatttgatta
    18001 tcttttgtta tttatatttt acaactataa gaaagccttg tcaaggattt gtgtaaacat
    18061 ttgattattt tttattatca atatataata aaaagagacc agtaagtaat gaaaagctta
    18121 aaaagattaa catctacatt tatccatagc aaaaaatcat aggattttaa gaatctgaca
    18181 actaggaaga aaactaatat gaccttatct aagaaacaat tacaactaag agctaaaata
    18241 ctggaaacag tttacacatt aggtcccatt tcacgaattg aaatcgctac taaaacagga
    18301 ataacacctg caacaactag cagtatcaca aatgatctga ttaaagaaaa tattctctta
    18361 gaacttggtg aagacgaaca cgatacaagc gtagggagaa aaaaaatcct gctagacatt
    18421 caagctaaac gattttacta cattggttgt gaactatctg aaaaacattt cacttttgct
    18481 ttaggagata atctaggaaa tatcttaaag gaagagaaag aaattgttac taaacagtta
    18541 atccaagaaa agggaaatca actcattaac cagacactaa agcaattctt aaataactgc
    18601 tctgattatg aaatagaagc aattggaatc gcgctacctg gtcgctattt agatgattat
    18661 aaaataacaa caaacaatcc tttatggcaa catatcgact tagaaatgat tcaatcccac
    18721 tttgataagc ctttgttttt ctctaacaat gttaactgta tggctatagg taaacgccta
    18781 tttagtcgcc aacagaatga ccctaatttt gcttattttc attttgctag aggtatgcac
    18841 tgttcttata tctacgacgg aaacatttat gggaaaggaa atctaatgat tggagaaata
    18901 gggcatacag ttgtttcatc tgagggagaa gaatgtagct gcgggcgcaa gggatgctta
    18961 caaacttttg ctggagaatc ttggttaatc aaaaaatcaa aaattctata tcatcaatct
    19021 ccctattctc tacttcctag tttagtcaaa aatgccgatg atattgatat ccaagttatt
    19081 ttaaccgctt atcaattagg tgacactgga ataatcacac ttatccatca agccttattc
    19141 tatcttagtc aaaccatttt aaatatcagt atgatgattg actctcaaaa aatctaccta
    19201 catagtcctt tactaacaaa tcaacacatc atccaaaaac tatatagtga aatgaactac
    19261 aaacccaaac tgctctataa tcgcctacct gaagtcatta ttgaacctta caatgatttt
    19321 acagccgcac actcagctat tgcactttgt ttgtatcata caattctaca ttcttaattt
    19381 tctattacat ttaataaata ggctaaaaag agggagtata tcataaaaag cgaataaacc
    19441 tgttttctga aaatcagaat tctgttttgt tcgctttctt tatatttgga attatactcc
    19501 tatctcccct ttttcataat gtatagtaaa atgaaataat aacaggac
//
LOCUS       DAVBCG010000025        19533 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBCG010000025 DAVBCG010000000
VERSION     DAVBCG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.025"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            70..798
                     /locus_tag="VQ495_000746"
     CDS             70..798
                     /locus_tag="VQ495_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2530731.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            791..1528
                     /locus_tag="VQ495_000747"
     CDS             791..1528
                     /locus_tag="VQ495_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2530732.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            1541..2251
                     /locus_tag="VQ495_000748"
     CDS             1541..2251
                     /locus_tag="VQ495_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530733.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIMSFLLFLPIAIFMQICCI
                     RFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVCIWIMG
                     FEFSLLPKLIKLKEQNLV"
     gene            2794..3015
                     /locus_tag="VQ495_000749"
     CDS             2794..3015
                     /locus_tag="VQ495_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2530734.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            3532..3771
                     /locus_tag="VQ495_000750"
                     /pseudo
     CDS             3532..3771
                     /locus_tag="VQ495_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3915..5144
                     /locus_tag="VQ495_000751"
     CDS             3915..5144
                     /locus_tag="VQ495_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2530735.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5164..7860
                     /gene="ppc"
                     /locus_tag="VQ495_000752"
     CDS             5164..7860
                     /gene="ppc"
                     /locus_tag="VQ495_000752"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2530736.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMIAQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    7943..8160
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8273..9277
                     /locus_tag="VQ495_000753"
     CDS             8273..9277
                     /locus_tag="VQ495_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2530737.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            9346..10212
                     /locus_tag="VQ495_000754"
     CDS             9346..10212
                     /locus_tag="VQ495_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2530738.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            10209..10967
                     /locus_tag="VQ495_000755"
     CDS             10209..10967
                     /locus_tag="VQ495_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530739.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            11057..12817
                     /gene="dnaG"
                     /locus_tag="VQ495_000756"
     CDS             11057..12817
                     /gene="dnaG"
                     /locus_tag="VQ495_000756"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2530740.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            12820..13929
                     /gene="rpoD"
                     /locus_tag="VQ495_000757"
     CDS             12820..13929
                     /gene="rpoD"
                     /locus_tag="VQ495_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2530741.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            13943..14272
                     /locus_tag="VQ495_000758"
     CDS             13943..14272
                     /locus_tag="VQ495_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2530742.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            14387..15430
                     /gene="cpoA"
                     /locus_tag="VQ495_000759"
     CDS             14387..15430
                     /gene="cpoA"
                     /locus_tag="VQ495_000759"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2530743.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            15446..16771
                     /locus_tag="VQ495_000760"
     CDS             15446..16771
                     /locus_tag="VQ495_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2530744.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            16802..16978
                     /locus_tag="VQ495_000761"
     CDS             16802..16978
                     /locus_tag="VQ495_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2530745.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            17037..17171
                     /locus_tag="VQ495_000762"
     CDS             17037..17171
                     /locus_tag="VQ495_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2530746.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            17235..18545
                     /gene="obgE"
                     /locus_tag="VQ495_000763"
     CDS             17235..18545
                     /gene="obgE"
                     /locus_tag="VQ495_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2530747.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            18555..18713
                     /locus_tag="VQ495_000764"
     CDS             18555..18713
                     /locus_tag="VQ495_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530748.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         6122 a         3257 c         4162 g         5992 t
ORIGIN      
        1 ggttcgctga ttgtttacaa aaacaataag aattaagcat aattttttgc tgtaaactaa
       61 ggagtagaga tggctatagt tgaaattata aatctaacaa aaagctttaa agatattgaa
      121 gttattcata acacttcatt ttatttaaat aagggaaaag tatatggttt tgttggacca
      181 aatggggctg gaaaaactac aattatcaaa atgattctag ggattttaaa accagattct
      241 gggaaaataa ctatttttaa tcaaaccgta gaacagaatt ccaaaaatat attatctagg
      301 ataggattag ttttgggacc ttcattttat ggtcatcttg atgcttataa aaatttgaag
      361 ttaatagcaa atatgaaagg gcttagttta gatactgaaa gacttaatga atatctatca
      421 atggtaggat taaaagatgt taaaaagaaa aaagtgaaaa atttttcaat gggcatgaaa
      481 caacgtctaa gtatagctgc atcattgtta ggtagtccag aaatattaat ttgggatgaa
      541 ccaatcaatg gacttgatcc acagggtgta attgaaatta gaagtttaat ccgttttcta
      601 caggagaaaa aaggtattac atttttaata tcaagtcata ttcttagtga actggataaa
      661 gtgatatctg atattataat tataaattat gggaaagttg aattttttgg ttcttgccac
      721 taccttttac aaaagtataa ttgtcgaaat ctggaagaag cctacttagc ttgtttagct
      781 ggaggtgaat atgattaaat tagagtttct aaagcagaaa aaaagcatct tatggtttgt
      841 tcttattttt ccaataattc tgaatgcttt gttatatata gacttaactt ttaggtatcg
      901 aggttattta ttggtgcatc aaaatgaact tgctttatct aattggcagt taatttttaa
      961 agaacaaaca atattttatt tttctgaact attttattta gttctatctt taataattta
     1021 cgaagtgttt gcagtagaat ttaaaaatga tgcatggtta accgtaattt cattgccatt
     1081 tagaaacaag tacacaataa attctaaatt actcattaca gtggtttaca catttacttt
     1141 ttggttgtct gattatatta gcttatacgt tattgggaag gctattgata acagtttaga
     1201 gattggtttg attttctttt taaaaacatt taccattcaa ttaatttcct cgttgatgat
     1261 aatgttatta tattttctaa ctttagtact tataaggaaa atttctggaa taataccaat
     1321 tggtattata atgatgattt taacaattag catatactac aacgattata attttaagat
     1381 ttacttacca tttacttatc tttctcatgc ttttagagtg actgaaagtc aattttatat
     1441 gattttacta tctaatataa taatcatcgt acttttttat atattaattc gtaaattgaa
     1501 cgaaagaagt tttgagatga aactttaata ggtaataaaa atgaagttac ttaaaaatga
     1561 attgattaag agtaaaatat ttttatttat tattgttgac atctgtattc agattctagt
     1621 cattctagct ataaaaacat atattttaga tatttcggca ccatactcag agcttgatta
     1681 taataagtat tggtatgttt tacacgcttt aatatatatg ccaatgattt ttcctataca
     1741 aattttgtat caaaatctaa gagaggcatt gattgaagat aataataatg gttggaatat
     1801 catggtgatt aacactaata atctcgtgaa aatcatctat ataaaagtaa cgataaatat
     1861 cgtgagatgt tttatttgtt attttgttta tacaattttt tctttaattc aattaggtgg
     1921 tatgggaact gatatgttat taaccaatat aatatttccc aatataatgt catttctgtt
     1981 atttttacct atagctatct ttatgcaaat ttgctgtata agatttgatt ccattttagc
     2041 aaaggcctta cctaatattt tgctgatact tattgtttta atcacgtttc agagtgattg
     2101 gaatatattt atacctgcaa cttattatta tacagaaatt cagtcaacta caaacttagg
     2161 tattaaatta ttagtttgta tctggattat gggatttgaa ttttcccttc ttccaaaact
     2221 aataaaattg aaagaacaaa atctagttta aatgttgctc gaaaattaaa taattgaata
     2281 cgattaacaa tgaaacaata caacgtgagt catgacttca aagtaattga ttttatgatt
     2341 ctggtttctt acgcttaatt aagcaagttg tattcacttt atcaaattgt tattactcta
     2401 aaaagcgtct aacagctata gagtgtctgt ttgcttctct ttacttttat tttaaataat
     2461 tcattaacct tccgtaagtg cccaataata aggtgtgttg aagaagtctg agataaaata
     2521 gttctcagtc acaaaaaaag ctatagattc ccattggtga acagctccct gtcaagtaga
     2581 cagtaaaata atataaaatc ttaagcgacc tgattcctga attccagtgg agtcaggttg
     2641 ttttttaacc gtctttctta atattgtctc acctataata gatatgctta tgagatatgt
     2701 ttaataaatt aattttagtt caaaatgtcc agttaggaca ctttgaatga tttcttagtt
     2761 ttatatatta tacttttctc aggagaaagc tagatgtaca gacgtttgag agatttgagg
     2821 gaagatcacg atttgaccca aaaacaaata gctaaaatac tttcgtttac aaactcagct
     2881 tatgctaaaa ttgaacgggg tgagcatacg ttaacagcag atgtattggt aactctctca
     2941 aacttttacg atgtcagtac agactaccta ttggaattga cagattttcc tgataaaatt
     3001 cgctttagaa aataatctcc tcaatttcat agagtgtgaa aatgaggaga ttttttattt
     3061 gttctttgaa aattaaatag tccaaaatgg tactttcctc atttgtggag caaatttgaa
     3121 tggctcgcca tgataagagc gattttaaag ccatcaataa aatagagcga tactttatat
     3181 gccatgatac aaatgatata caatgatact tctgaccgtt caggctgcca tcgtaaaaga
     3241 gcagcaagtg aaattcttat gatgacttca tcagtcatgc cacgttgaat gtgtgagttt
     3301 gttagagaaa cgcaattaat cctcaaaagg ttccccgaac cttttgagtt ccacagacgc
     3361 atcacgtgga gtgtgtaagc ttgttgctaa aagcgtaaaa accttggaac gaaaggaata
     3421 atagacttcc tgcgaaacaa aaatacaata caataaaact atggatgatg aagcaagtaa
     3481 acaattaagc gatagccgtt tcaagagcct tgtaggtgtt cagcgcacga gatgttagct
     3541 gtgttaaaaa cagcttatca acgcaacgta aacgcgcaaa aggtggacga aaaaccaaat
     3601 taagcctaga cgatctcctc atggtaacta ttcaatacat gcgagaatag cgcacttatg
     3661 aacaaattgc ggctgatttt ggcattcacg aaagcaacat aattagtcgg aggtcaatgg
     3721 attgaagcaa ctcttattca aaatggtttt acgatttcaa attctgcctt aattctgtaa
     3781 aaacagtaaa attcgaagga ttgtaaggta agagtttttt tctttctgaa aaaatggtat
     3841 aatagcaatc aaaactagaa aataaaacgg aatttggaac agatttgtct gtatcctagt
     3901 agagtggtga tactatgaag attagtaaga ggcacttatt aaattattcc atcttgattc
     3961 cctacttgct tttatctatt ttgggcttga ttgtggtcta ttcgaccacc agtgctattt
     4021 taattgaaga aggcaagagc gccttgcagt tggttcgaaa ccaaggaatc ttttggattg
     4081 ttagtttgat actgattgcc ttaatttata aattgagact agattttttg agaaatgagc
     4141 gactaatcat tttagttata ttaatagaaa tgcttttatt gttcttggct cgttttattg
     4201 gtatttcagt aaacggggca tacggttgga tttcggttgc aggagtaact attcagccag
     4261 ctgagtactt aaaaatcatt attatttggt atttagctca ccgattctcc aaacagcaag
     4321 aagaaatagc tacttatgat tttcaagttt tgactcaaaa tcaatggctt ccccgtgctt
     4381 ttaatgattg gcgattcgtt ctcctagttc tgattggaag tttgggaatt ttccctgatt
     4441 taggaaatgc gactatttta gtcttggttt ccttgattat gtatacagtt agtggaatcg
     4501 cttatcgctg gttttcaacc attctggcgc tcgtatctgc cgcttctgtc tttgtcttga
     4561 ccactatcag cctaatcggt gttgagacct tttcaaaaat tccagtattt ggctatgtag
     4621 ccaaacgctt tagtgccttt tttaatcctt ttgccgatcg tgctgatgca ggtcaccagt
     4681 tagctaattc ttattttgcc atggtcaatg gtggttggtt tggtctaggt cttggaaact
     4741 cgattgaaaa acgaggttat ttgccagaag ctcatacaga ctttgtcttt tctatcgtga
     4801 ttgaagaatt tggctttgtt ggtgccagtc ttattttagc tctcttgttt ttcatgattt
     4861 tgcggattat cttggtcggt attcgagcgg agaatccttt caatgccatg gttgcactcg
     4921 gtgtcggagg gatgatgttg gttcaggtat ttgtcaatat cggagggatt tcgggcttga
     4981 ttccatctac aggagtaacc ttccccttct tatcccaggg tggaaatagt cttctagtct
     5041 tatcagtggc agtagccttt gtcttaaata ttgatgccag tgaaaaacgc gctaagttgt
     5101 accgagaatt ggaaaatcaa ccaatgaacc ttctgttgaa gtaggataaa gaaaggatag
     5161 tttatgtctc ttcaaaaatt agaaaattct agtaataaaa gtgttgtgca agaagaagtc
     5221 ttgattctaa cagaattact ggaagatatt actaaaaata tgcttgcccc agagaccttt
     5281 gaaaaaataa tacagttgaa agaattatca acgcaggaag attatcaagg tctaaaccgt
     5341 ctagtgacta gcttatcaaa tgatgaaatg gtctatattt cacgctattt ctctatcttg
     5401 cctcttttga ttaatatttc agaggatgtg gatttagctt atgaaatcaa tcatcaaaat
     5461 aatattgatc aggactattt aggtaaatta tctacaacga ttaaattggt agcagaaaag
     5521 gaaaatgccg ttgagatcct agaacacttg aatgttgttc cggttttgac agcccatcca
     5581 acacaagtgc aacgcaaaag tatgttggat ttaacaaatc atattcatag tcttttgcgt
     5641 aaataccgtg atgttaagtt ggggttgatc aataaagata aatggtacaa tgatttgcgt
     5701 cgttacatcg aaattatcat gcagacagac atgattcgtg agaaaaaatt aaaagtgact
     5761 aacgaaatca cgaatgctat ggaatattat aacagctcct ttttgaaagc tgtacctcat
     5821 ttgacgacgg agtataagcg cttagcgcaa gcgcatggtc tgaatttaaa acaggctaaa
     5881 ccaatcacca tgggtatgtg gataggtggt gaccgtgatg gaaatccatt tgttacagca
     5941 aagaccttga agcagtctgc actcactcaa tgtgaagtca tcatgaacta ctatgataaa
     6001 aagatttacc aactttatcg tgaattttct ctttcaacta gcattgtcaa cgtcagcaag
     6061 caagtcagag aaatggctcg tcaatccaag gataactcga tttaccgcga aaaagagctt
     6121 taccgtcgtg ccttgtttga tattcaatca aaaattcagg caactaaaac ctatctgatt
     6181 gaggatgaag aagttgggac tcgttatgaa accgccaatg atttctacaa ggatttgatt
     6241 gccattcgag attctctact agagaataag ggcgagtcct tgatttcagg tgattttgtg
     6301 gaattattgc aggcagtaga gatatttggt ttttacctag catcaattga tatgcgacaa
     6361 gactctagcg tctatgaagc ctgtgtggca gaactcttga aatcagcagg aattcattct
     6421 cgttatagcg agttgagcga agaagaaaag tgtgaccttc ttttgaaaga attagaagaa
     6481 gatccccgaa ttctttctgc gactcacgca gaaaaatcag aattattagc aaaagaacta
     6541 gctattttta agacggctcg tgttttgaaa gataagttgg gagatgatgt catccgtcag
     6601 accatcattt cacatgcaac caacctttct gatatgctag aattagctat tctgttaaaa
     6661 gaagtaggac tggtggatac ggaaagggcg cgtgttcaga ttgttcccct ttttgaaaca
     6721 attgaagact tggatcattc agaggaaaca atgagaaaat atctttctct tagccttgcc
     6781 aaaaaatgga ttgactcacg aaataactac caagaaatca tgcttggcta ctctgacagt
     6841 aataaagatg gcggttactt gtcatcatgt tggaccctct acaaggctca acaacaattg
     6901 actgctattg gagatgaatt tggcgttaag gttaccttct tccatggtcg tggtggtact
     6961 gtcggtcgtg gtggtgggcc aacctatgaa gccattacat ctcaaccgct caagtctatc
     7021 aaggatcgta tccgtttgac ggagcagggt gaagtaattg ggaataaata cggtaacaaa
     7081 gacgccgctt actataacct tgaaatgcta gtatcggcag ctattaaccg tatgattgct
     7141 cagaagaaga gcgataccaa taccccaaat cgttatgaag ccattatgga tcaagtagtg
     7201 gaccgtagtt acgatatcta ccgtgatttg gtctttggta atgagcattt ctatgattat
     7261 ttcttcgagt caagtccaat caaggctatt tcaagtttta atattggttc tcgtccagcc
     7321 gctcgtaaga ctattactga aatcggtggt ttgcgtgcca tcccttgggt attctcatgg
     7381 tcacagagtc gtgttatgtt ccctggatgg tacggggttg gttcaagctt caaggaattt
     7441 atcaataaaa atccagagaa tattgctatc ttacgagata tgtaccaaaa ttggcctttc
     7501 ttccaatcgc ttctttcaaa tgttgatatg gttttgtcaa aatcaaatat gaatattgct
     7561 tttgaatatg ctaaactttg tgaagacgag caagttaagg ccatctatga gattatttta
     7621 aatgaatggc aagttactaa gaacgttatc ttggctattg aaggacatga cgaactctta
     7681 gctgacaatc catatctaaa agctagtctg gattaccgta tgccttactt taatattctc
     7741 aactatattc agttagagtt gattaaacgc caacgtcgtg gagaattgtc cagtgatcaa
     7801 gaacgattga ttcatatcac catcaacgga attgcgacag gattgcgtaa ttcaggttga
     7861 taattttcaa gagtgaatgc taaaagtgaa tatcaaaaaa attctaatag actattgaca
     7921 agtagtttaa aaatgatata atttaaccat tcagaaaagt aatcatacaa actttttaga
     7981 gagtctgtgg tagctgaaaa cagataagtg gcgatgatga aaattgggct gaatgctatt
     8041 tagaatttga aattataaaa attcggtaag cacaccttac agtgcatctc gttattgcga
     8101 gactgagcga tagggaaatt ccctataatt gaggtggtac cgcgcatcga cgtcctcaca
     8161 caagtttttt gtgtgaggat ttttttgatg gaggttagta tggaaagaaa acgatggcgt
     8221 cgcttgttta gataagtgaa atatgttaaa ggaaataaaa aggagaaaca gaatgaaaaa
     8281 taaacgttta attggaatta ttgctgcatt agcagtctta gtagcaggaa gcttgattta
     8341 ttcttcaatg aataaatcag aagctcagaa taataaggat gagaagaaaa taaccaagat
     8401 tggtgtgctt caatttgtga gccatccatc ccttgatttg atttataaag ggatccaaga
     8461 tggacttgca gaagaaggat ataaagatga tcaagttaaa attgatttta tgaactcaga
     8521 aggtgatcaa agtaaggttg cgacaatgag taaacaattg gttgcaaatg ggaatgacct
     8581 tgtggttggt attgcaacac cagcagccca agggttggct agtgcaacaa aagacctacc
     8641 agttatcatg gccgctatta cagacccaat tggtgctaac ttggttaaag atttgaaaaa
     8701 accaggtggt aacgttacag gggtatctga ccacaatcca gctcaacaac aaattgaact
     8761 catcaaggct ctgacaccga atgtgaaaac aatcggagct ctttactcaa gtagcgaaga
     8821 caattcaaaa acacaggttg aagaatttaa ggcttatgct gaaaaagcag gtctgacagt
     8881 ggaaacattt gcagttcctt caacaaatga aattgcctca actgtcactg ttatgactat
     8941 caaggtagat gctatttggg ttccaattga taacaccatt gcatcaggat ttccaacggt
     9001 tgtctctagc aatcaaagtt ctaagaaacc aatttatccc agtgcgacag ctatggtaga
     9061 agtaggtggt ttggcatcag ttgtaattga ccaacatgac cttggtgtgg caacaggtaa
     9121 aatgattgtg caagtcttga aaggtgcaaa accagccgat accccagtca atgtcttttc
     9181 aactggtaag tcagtcatca ataaaaaaat agcacaagaa ctaggtatta ctattcctga
     9241 gtctgttctc aaagaagcag gacaagtcat cgaataaata aggtagggag gaggagactc
     9301 ttcccatttt ttaaaaagat gaatatagaa aggattaaat aaaatatgat tgtttccatt
     9361 atttctcaag gatttgtctg ggctattcta ggtctgggaa tctttatgac atttaggatt
     9421 ttaaactttc cagatatgac gacagaaggt tccttccctc ttgggggagc tgttgctgtc
     9481 actttgataa ccaaaggcgt gaacccattt ttagcgacac ttgttgctgt aggagcaggt
     9541 tgtttggctg gaatggcagc aggccttctt tatacaaaag ggaagatccc aaccttgctc
     9601 tcagggattt tggtgatgac ttcttgtcac tcaatcatgc tcttgattat gggacgtgcg
     9661 aatttaggcc tgcttggaac caagcaaatt caggatgttt tgccttttga ttcggatttg
     9721 aatcaactct tgacaggtct catctttgtg agcattgtta ttgctctcat gctctttttc
     9781 ttggacacta aactcggaca agcctatatt gctacagggg ataatcctga tatggctaga
     9841 agtttcggga ttcatactgg acgcatggag ctcatgggct tggtcttatc aaatggtgtg
     9901 attgcccttg caggtgccct cattgctcag caagaaggct atgccgatgt gtctcgaggg
     9961 atcggggtta tcgttgtggg gcttgcaagt ttgattattg gagaagttat tttcaagagt
    10021 ttgagcttgg cagagcgttt ggttactatc gttgtaggtt ctatcgctta tcaattttta
    10081 gtgtgggcag ttatcgcact tggctttaat acaagttacc ttcgtttata cagtgccttg
    10141 attttagcag tctgcctcat gattccaaca tttaagcaaa caatcttgaa aggagccaag
    10201 ttaagcaaat gacagcaatt gtagaattaa aaaatgcaac caaaattgtt aaaaatggct
    10261 ttgatgaaga aaagattatt ttaaatgatg tttccttaga aatttttgaa cgggacttta
    10321 tcacgatttt gggcggaaat ggtgctggaa aatcaaccct ctttaacact atagcaggga
    10381 ccttatcact aactagtgga actatccgta ttttaggtga agatctcact aagttttcac
    10441 ccgagaagcg tgccaagtac ctgtctcgtg tcttccaaga tccaaagatg gggacagctc
    10501 cccgtatgac ggtcgctgaa aatcttttaa tcgccaagtt tcgtggtgag aagcgtggat
    10561 tgttaccacg acgcttgact agctataagg atgaatttca ggcaaccatt gaaaaagtag
    10621 gaaatggtct tgagaaacac ttgaatacac cgattgagtt cttatcaggt ggacaaagac
    10681 aggctttgag tctcttgatg gcaaccttga agcgacctga attactcctg ttagatgagc
    10741 atactgctgc cctggatcca aagactagtg ttgctttgat ggaattgaca gatgaatttg
    10801 ttaagaaaga tcagctaaca gcccttatga ttactcatca tatggaagat gctctcaaat
    10861 acggcaatcg cttgattgtc atgaaagaag gacggattat ccaagattta aaccaagaag
    10921 aaaaagcaaa aatgaaaatc tctgattatt atcaactctt tgaataaagt agggaaaatg
    10981 agtgaaatgg tttacttttt ttctgaaata aagtatacta tataaagtaa actatgataa
    11041 catggaggta ttgtgtatgg ttgacaaaca agtcattgaa gaaatcaaaa acaatgccaa
    11101 cattgtggaa gtcataggag atgtgatttc tttacaaaag gcaggacgga actatctagg
    11161 gctctgtcct tttcatggtg aaaaaacacc ttctttcaac gttgtagagg acaagcagtt
    11221 ttaccactgt tttggttgtg gtcgctcagg tgatgtcttt aaattcatcg aggagtacca
    11281 aggggttacc tttatggagg ctgtccaaat cttaggtcag cgtgtcggga ttgaggttga
    11341 aaaaccgctt tatagtgaac agaagccagc ctcgcctcac caagctcttt atgatatgca
    11401 cgaagatgcg gctaaatttt accatgctat tctcatgaca acgactatgg gcgaagaggc
    11461 cagaaattac ctttatcagc ggggtttgac agatgaagtg cttaaacatt tttggattgg
    11521 tttagcacct ccagaacgaa actatctcta tcaacgtttg tctgatcagt atcgtgaaga
    11581 ggatttactg gattcaggcc tgttttatct ctcggatgcc aatcaatttg tagacacctt
    11641 tcacaatcgc attatgtttc ccctgacaaa tgaccaagga aaggtcattg ccttctcagg
    11701 tcgtatctgg caaaaaacgg attcacaaac ttctaagtat aaaaacagcc gatcgactgc
    11761 aatttttaac aaaagttacg aattatatca tatggatagg gcaaaaaaat cttctggaaa
    11821 aactagtgag atttacctga tggaaggatt catggatgtt attgcagcct atcgggctgg
    11881 aatcgaaaat gctgtggcgt cgatgggaac ggccttgagt cgagagcatg ttgagcatct
    11941 gaaaaggtta accaagaaat tggttcttgt ttacgatgga gataaggctg ggcaagccgc
    12001 gatattgaaa gcattggatg aaattggtga tatgcctgtg caaatcgtta gcatgcctga
    12061 taacttggat cctgatgagt atctacaaaa aaatggtcca gaagacttgg cctatctatt
    12121 aacaaaaact cgtattagtc cgattgagtt ctacattcat cagtacaaac ctgaaaacag
    12181 tgaaaatctg caggctcaga ttgagtttat tgaaaaaata gctcccttga ttgttcaaga
    12241 aaagtccatc gctgctcaaa acagctatat tcatatttta gctgacagtc tggcgtcctt
    12301 tgattatacc cagattgagc agattgttaa tgagagtcgt caggtgcaaa ggcagaatcg
    12361 catggaaaga atttccagac cgacgccaat caccatgcct gtcaccaagc agttatcggc
    12421 tattatgagg gcagaagccc atctactcta tcggatgatg gaatcccctc ttgttttgaa
    12481 cgattaccgt ttgcgagaag actttgcatt tgctacacct gaatttcagg tcttatatga
    12541 cttgcttggc cagtatggaa atcttcctcc agaagtttta gcagagcaga cagaggaagt
    12601 tgaaagagct tggtaccaag ttttagctca ggatttgcct gctgagatat cgccgcagga
    12661 acttagtgaa gtagagatga ctcgaaacaa ggctctcttg aatcaggaca atatgagaat
    12721 caaaaagaag gtgcaggaag ctagccatgt aggagataca gatacagccc tagaagaatt
    12781 ggaacgttta atttcccaaa agagaagaat ggagtaataa tggcaacaaa acaaaaagaa
    12841 gtaacaacat ttgacgtaca ggtagcagaa tttatccgta atcataagca aaaagggaca
    12901 gcaacagatg atgaaatcaa tgcaagtctg gttattcctt ttaccttgga cgctgatggg
    12961 attgaagatc tcttgcaacg gattcaggat gcagggattt ctatcacaga taacgaagga
    13021 aatccaagtg cgcgtgttct tagcaatgaa gaagaaccag aactcagcga tgaggacttg
    13081 attggatcaa cttctgctaa ggtcaatgac cctgtccgta tgtacttgaa agaaataggg
    13141 gtcgttcctc tcttgaccaa tgaagaggag aaagagttgg cactggctgt tgaagctggc
    13201 gatatcgaag ccaaacaacg tcttgcggaa gccaatcttc gtttggttgt ttccattgcc
    13261 aaacgctatg tcggtcgtgg catgcagttc cttgacttga ttcaagaagg aaatatgggc
    13321 ttgatgaagg cggttgacaa gtttgactat tctaaagggt tcaagttttc aacttatgca
    13381 acttggtgga ttcgtcaggc tatcactcgt gctattgcgg accaagctcg taccatccgt
    13441 atcccagttc acatggttga aactatcaat aaattggttc gtgaacagcg gaatctcctt
    13501 caagaattgg ggcaagatcc gacaccagaa cagattgctg aacgaatgga tatgacacct
    13561 gataaggttc gtgaaatctt gaagattgcc caagaaccag tatctcttga aactcctatc
    13621 ggtgaagagg acgatagcca ccttggagac tttatcgaag atgaagtgat tgaaaatcca
    13681 gtggattata cgactcgtat cgtcttgcgt gagcaattgg atgaaatctt agatactctt
    13741 acagaccgtg aagaaaatgt tctgcgtcta cgttttggac tagatgatgg aaaaatgcgc
    13801 acacttgaag atgtggggaa agtctttaac gtaactcgtg agcgtatccg tcagattgaa
    13861 gcaaaggctt tgagaaaact acgccaacca agtcgcagca aaccgcttcg tgattttatt
    13921 gaagactaag agtgaggaaa atatggctta tacagaagag caaattgaaa acatcaaaac
    13981 acggatttta acagccttgg aagaagtcat cgaccctgag ttgggaatcg atattgtcaa
    14041 tcttggtttg atctatgaga ttcgttttga cggtgacaca gggcaaacag agattgacat
    14101 gactttgaca actatgggtt gtcccttagc agaccttttg acagaccaga tttatgatgc
    14161 tatgatagag gttccagaag taacggatac tgaggttaaa ttagtctggt atccagcctg
    14221 gacagttgaa aaaatgagtc gctatgcccg cattgcccta ggcattaagt aaacatataa
    14281 aagcatgtga gagactagag gaaaagcgag gaaatttccc ctcttttcct ctagtctctc
    14341 ctttcttttg ctgattttat tcaaagaaaa tgatataata gtagttatgg agaaaaagaa
    14401 attacgcatc aatatgttga gttcaagtga gaaagtagca ggacagggag tttcaggtgc
    14461 ttaccgtgaa ttagttcgtc ttcttcaccg tgctgccaag gaccaattga ttgttacaga
    14521 aaatcttcca atcgaggcag atgtgactca ctttcatacg attgattttc cctattattt
    14581 atcaaccttc caaaagaaac gctcagggag aaagattggc tatgtgcatt tcttgccagc
    14641 tacacttgaa ggaagtttga aaattccatt tttcttaaag ggaattgtga aacgctatgt
    14701 attttctttt tacaaccgga tggagcactt ggttgtggtc aatcctatgt ttattgagga
    14761 tttggtagca gctggtattc cacgtgaaaa agtgacctat attcctaact ttgtcaacaa
    14821 ggaaaaatgg catcctctac cacaagaaga ggtagtcaga ctgcgcacag atcttggtct
    14881 tagtgacaat cagtttatcg tagtaggtgc tgggcaagtt cagaaacgta aagggattga
    14941 tgactttatc cgtctggctg aggaattgcc tcagattacc tttatctggg ctggtggctt
    15001 ctcttttggt ggtatgacag atggttatga acactataag aaaattatgg aaaatccccc
    15061 taaaaatttg atttttccag gcattgtatc gccagagcgg atgcgcgaat tgtatgctct
    15121 agcggatctt ttcttgttgc ctagttacaa tgagctcttt cctatgacta ttttagaagc
    15181 tgcgagttgt gaggctccta ttatgttgcg tgatttagat ctctataagg tgattttgga
    15241 gggaaattat cgggcgacag cgggtagaga agagatgaaa gaggctattt tggaatatca
    15301 agcaaatcct gctgtcttaa aagatctcaa agaaaaggct aagaatattt ccagagagta
    15361 ttctgaagag catctgttac aaatctggtt ggacttttat gagaaacaag ccgctttagg
    15421 gagaaagtaa aaagtgaggt aatctatgcg aattggttta tttacagata cctattttcc
    15481 tcaggtttct ggtgttgcga ccagtattcg aaccttgaaa acagaacttg aaaagcaggg
    15541 acatgctgtt tttatcttta cgacgacaga taaggatgtc aatcgctacg aagattggca
    15601 aattatccgc attccaagtg ttcctttctt tgcttttaag gatcgtcgct ttgcctaccg
    15661 aggttttagc aaggcacttg aaattgctaa acagtatcag ctagatatta tccatactca
    15721 gacagaattt tctcttggcc tgttggggat ttggattgcg cgtgaattga aaattccagt
    15781 catccatacc tatcacaccc agtatgaaga ctatgtccat tatattgcta aggggatatt
    15841 gatccggccg agtatggtca agtatctggt tagaggtttc ctgcatgatg tggatggggt
    15901 tatttgccct agtgagattg tccgtgactt gctatctgat tataaggtca aggttgaaaa
    15961 acgggtcatt cctactggga ttgaattagc caagtttgag cgtccggaaa tcaagcagga
    16021 aaatttgaaa gaactgcgta gtaaactagg gattcaagat ggtgaaaaga cgttgcttag
    16081 tctttcgaga atctcctatg aaaaaaatat tcaagcagtt ttagcagcct ttgctgatgt
    16141 tctgaaagag gaagacaagg ttaaactggt agtagctggg gatggccctt atctgaatga
    16201 cctcaaagag caagcccaga acctagagat tcaagactca gtcatcttta cagggatgat
    16261 tgctcctagt gagacggctc tttactataa agcggcggat ttcttcattt cggcatcgac
    16321 aagcgaaacg caaggtttga cctacttgga aagcttagcc agtggaacac ctgtcattgc
    16381 tcacggaaat ccttatttga acaacctcat cagtgataaa atgtttggag ccttgtacta
    16441 tggagaacat gatttggctg gtgctatttt ggaagccctg attgcaacac cagacatgaa
    16501 tgagcatacc ttatcagaga aattgtatga gatttcagct gagaactttg ggaaacgagt
    16561 gcatgagttt tatctggatg ccattatttc aaataacttc cagaaagatt tggctaaaga
    16621 tgatacggtc agtcagcgta tctttaggac agttttgtat cttccgcaac aggtggttgc
    16681 tgtacctgta aaaggatcta gacgcatgtt gaaggcttca aaaacacagt tgatcagtat
    16741 gagagactat tggaaagacc atgaagaata gaaagaggaa cagctatgaa aaaaacaatt
    16801 aatgagaagc ggtcgtgata aaaagattgc gggtgtttgt gctggggtgg cccattatct
    16861 ggatatggat ccgactatcg ttcaagtcat ttggggtgtt cttacttgct gttacggagc
    16921 tggaattgta gcttacatta ttttatggat tatcgcacca gtagcaactg actattgaaa
    16981 actagctcaa ttcatgctaa gataatagaa aatagaatgt aacgaaggag agaaaaatgg
    17041 catttggaga taatggaaat cgtaaaaaaa ctatgtttga gaaaataacc ttgtttatcg
    17101 tgattatcat gctagtagca agtttattgg gaatttttgc aactgcaatt ggtgccctca
    17161 gtaatctata aaatagattc aagaaaattt agtgactggg atttcccagc ccttttttaa
    17221 agtgagaaga aataatgagt atgtttttag atacagctaa gattaaggtc aaggctggta
    17281 atggtggcga tggtatggtt gcctttcgtc gtgaaaaata tgtccctaat ggaggccctt
    17341 ggggtggtga tggtggtcgt ggaggcaatg tggtcttcgt tgtagacgaa ggactacgta
    17401 ccttgatgga tttccgctac aatcgtcatt tcaaggctga ttctggtgaa aaagggatga
    17461 ccaaagggat gcatggtcgt ggtgctgagg accttagagt tcgagtacca caaggtacga
    17521 ctgttcgtga tgcggagact ggcaaggttt taacagattt gattgaacat gggcaagaat
    17581 ttatcgttgc ccacggtggt cgtggtggac gtggaaatat tcgtttcgcg acaccaaaaa
    17641 atcctgcacc ggaaatctct gaaaatggag aaccaggtca ggaacgtgag ttacaattgg
    17701 aactaaaaat cttggcagat gtcggtttag taggattccc atctgtaggg aagtcaacac
    17761 ttttaagtgt tattacctca gctaagccta aaattggtgc ctaccacttt accactattg
    17821 taccaaattt aggtatggtt cgcacccaat caggtgaatc ctttgcagta gccgacttgc
    17881 caggtttgat tgaaggggct agtcaaggtg ttggtttggg aactcagttt ctccgtcaca
    17941 tcgagcgtac acgtgttatc cttcacatca ttgatatgtc agctagcgaa ggccgtgatc
    18001 catatgagga ttacctagct atcaataaag agctggagtc ttacaatctt cgcctcatgg
    18061 agcgtccaca gattattgta gctaataaga tggacatgcc tgagagtcag gaaaatcttg
    18121 aagaatttaa gaaaaaattg tctgaaaatt atgatgaatt tgaagagtta ccagctatct
    18181 tcccaatttc tggattgacc aagcaaggtc tggcaacact tttagatgct acagctgaat
    18241 tgttagacaa gacaccagaa tttttgctct acgacgagtc cgatatggaa gaagaagctt
    18301 actatggatt tgacgaagaa gaaaaagcct ttgaaattag tcgtgatgac gatgcgacat
    18361 gggtactttc tggtgaaaaa ctcatgaaac tctttaatat gaccaacttt gatcgtgatg
    18421 aatctgtcat gaaatttgcc cgtcagcttc gtggtatggg ggttgatgaa gcccttcgtg
    18481 cgcgtggagc taaagatggg gatttggtcc gcattggtaa atttgagttt gaatttgtag
    18541 actaggagac tggtatggga gataaaccga tatctttccg agatgcggat ggtaattttg
    18601 tttccgccgc agacgtttgg aatgaaaaga aattggaaga actatttaat cgtctcaatc
    18661 caaatcgtgc cttgagattg gcacgaacta aaaaggaaaa tccatctcag taaagaagct
    18721 aaaaaatccc gtgcctcatc agacacggga ttttgtggta cgacaggcat gtatagcaaa
    18781 ctgaatctgg aatagcacag catatcttct aaaatattgt tataaattga tttgaatttc
    18841 aaaattaaat tgtttgattc ttatctcaat ttgttatagt atatctgatg tcaaagttct
    18901 cggcgagtca aatagcgatt cccaagcctg actatcgtga ggtagcggat taaaatggtc
    18961 tggggataga ccgttttaag tctgacgctg gaaataagaa ttgtcagaag aagggatagc
    19021 gaaatcgtgg ctctacgaac aggaacgtga taataaggcg tatatagcgg ataagagggc
    19081 ctcaaactct aaagtccaaa aaggtagtcg taacctatat gcgtaaatca cgagagtaat
    19141 tgaattcgta ctaagatttt ctattttcac tgtaaccttt taacgtcctt atatcttgta
    19201 tacacgagga aagatgtacg acttatcccg tgaggtctat cactataaag agaaaacgac
    19261 agatagaagt gatcctgagt cacggttatc tttaaaatag attgtctgat aggacggtat
    19321 gtataaaacg cttctgtgaa ctgagagaag ggggagaagt tcttgctaaa atttagttga
    19381 acagccgtat tccgatactt agataagaga tctagtctta gctcctactc agttttaggg
    19441 gataaaaaag gggaaatagc gattcgagaa agattatact cttcgaaaat ctcttcaaat
    19501 cacgtcaata tcgccttgcc gtatgtgtag gat
//
LOCUS       DAVBCG010000026        18874 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBCG010000026 DAVBCG010000000
VERSION     DAVBCG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.026"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(146..397)
                     /locus_tag="VQ495_000765"
     CDS             complement(146..397)
                     /locus_tag="VQ495_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530749.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYIENVYLDSLSVKQKFKFIFDFGDEWRFEC"
     gene            complement(687..2567)
                     /locus_tag="VQ495_000766"
     CDS             complement(687..2567)
                     /locus_tag="VQ495_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2530750.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2561..3430)
                     /locus_tag="VQ495_000767"
     CDS             complement(2561..3430)
                     /locus_tag="VQ495_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2530751.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3514..6159)
                     /locus_tag="VQ495_000768"
     CDS             complement(3514..6159)
                     /locus_tag="VQ495_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2530752.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(6250..7530)
                     /locus_tag="VQ495_000769"
     CDS             complement(6250..7530)
                     /locus_tag="VQ495_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2530753.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7701..9785
                     /locus_tag="VQ495_000770"
     CDS             7701..9785
                     /locus_tag="VQ495_000770"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2530754.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAT
                     SQNYRQLFDTETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKVCQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(9825..11504)
                     /locus_tag="VQ495_000771"
     CDS             complement(9825..11504)
                     /locus_tag="VQ495_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2530755.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12260..13489
                     /gene="arcA"
                     /locus_tag="VQ495_000772"
     CDS             12260..13489
                     /gene="arcA"
                     /locus_tag="VQ495_000772"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2530756.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13547..14563
                     /gene="argF"
                     /locus_tag="VQ495_000773"
     CDS             13547..14563
                     /gene="argF"
                     /locus_tag="VQ495_000773"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2530757.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14728..15675
                     /gene="arcC"
                     /locus_tag="VQ495_000774"
     CDS             14728..15675
                     /gene="arcC"
                     /locus_tag="VQ495_000774"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2530758.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15884..17395
                     /locus_tag="VQ495_000775"
     CDS             15884..17395
                     /locus_tag="VQ495_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2530759.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17417..18748
                     /locus_tag="VQ495_000776"
     CDS             17417..18748
                     /locus_tag="VQ495_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2530760.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKCVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         5298 a         4277 c         3894 g         5405 t
ORIGIN      
        1 tgaaatacta agcctacaca gactgatttc aattttacca ttcttcatag tcaaaaccat
       61 cataatctgg atattgttct ggcaacgttc caaccgaacg tacgagataa gcttcttcgt
      121 cctctgtctc gatttctctc agcacttagc attcaaaacg ccattcatca ccgaagtcga
      181 aaataaactt aaatttttgt ttgacactta ggctatccag atagacattt tctatgtaac
      241 gttcttcaac atcgtcacta acaaagctaa gaaagtaaga atctgcatga ctccactcaa
      301 cattatccat gaaaaatgca tgagcatgat catttgcaaa atcgaaactc cataaaatca
      361 catctgctag ttccgctaac gtttttgttt ttggaatcta tatatgtcgg tagcaacctt
      421 ttttaatttg acggaaatga tccaagcttc tacagatgct ggcttttcag cttctatttc
      481 ttgttttact aattttaact gtagcatttg aaaccagata attcttctgt ttcacgcgaa
      541 acaccctgcg ccaagcaagg tgttcttgtt tttatactca atgaaaatca aagagcaaac
      601 taggaagcta gctgcaggct gctcaaaaca ctgttttgag gttgtagatg gaagctgacg
      661 tggtttgaag agattttcga agagtattaa gtcgtataaa tcgtcgaagc tgtcgcaatg
      721 gtatgccact ggttagctgt agttgctgcg aaatccttgt ctgcgtagaa gtcagtaaat
      781 ggtaggattt caacttccag ctcgtcgatg cggtcaagct ggccagccag ataaacctcg
      841 atacggcttt ctgctcgttt gatgcgttgc aagagtccgc ccatacggat gtcaactgta
      901 tccaaaccaa agaccttgtt ttctttcagc cattggtgac taaagagggc atggaagtct
      961 tcaatttggc ttctaagttc tggtaattct tgtctggcga tttgttgtaa actttcttta
     1021 tcatccgctt ggtaggcctg acgaatgcgt cgtcccacat ctactttgct acttaaaata
     1081 gcattcaact gggcctgagt ttcaaagaga taagcatagt ttccagcttt ttctttaatg
     1141 ttagcaagcg tctcagcagc ctgagcgaag tgcggtttgt cctgttcagg tgtcatgtgt
     1201 tgatcaagaa tcggacaaag aatatcctga taaaaaacat agcggttggg attgataccg
     1261 ctgagattgc ctggtaggtc tggtaagagg ttggcaaggt caatctgcat aaaatcctca
     1321 accgttagac cagtattggt cttgaagtgc gcagacaaac catctaggtc attgcgatag
     1381 ctgagttctg cccagatttg caagcttggt aggatagaga actgggcagt ttcaccacca
     1441 ttgtctcccc aacccgttac gatgacttct ttaatctgat tggcacggca ggctttatta
     1501 gcctcgatag ccactagacg gctaaaatgg ttgtgaggtg taaagccaat ccacttccaa
     1561 gctccccctg caaatgcaag gtcatggcta atcttgtgat gattgcggaa attacggttg
     1621 tatttttcct cgctatcctg ataataatcc cagtaaacca gagtcacacg gtctttgaga
     1681 cggtctaggt agacacgagt ttcctctgga atttccacat cacggtcgta ctggccatcc
     1741 gctgacatga gtttgaagaa catatcactc cacatctggc agtggaaacc atatttgtca
     1801 gcaatatcca gcacgcgctc caagtgttgg cacatgagga gactacgatc cacaacaccg
     1861 ttcaaaatca ggtagcgtcc caaaccaacc aagtgggctt cgtccatccc gatattgacc
     1921 ttgcgagtct tcagtttaga caacgtggca aacatgccat caatcaggtc ataaaccttt
     1981 tcttcgccaa tgagaagaat gtcctctaca tcacggagct cctgcacttc cttgacaccc
     2041 catttgacaa aggccgacaa gtgggccaag gtctggatgc atggtacaaa ggtcatgtca
     2101 aactgttgag cataggcttc gatttcctgc aactcctctg ctgaataagc tccacggaaa
     2161 tagccaaagt aaggctgccc ttcaatctgg taagtgtctt ccatgtaaag ttcaaaggtt
     2221 gagtagccca tgagagccaa gacctcaatc atctgcttgg cagaagccac attcagcacc
     2281 gcatttcgag aacagtcaac catgtaagcc aaatcttcgt aagctgcttg ttcctcaatc
     2341 tctactttat cagcttctgc tagaactgtt accaacaagg ataaggcacg ataaagttgg
     2401 tgaggtttgc ggtaggtcaa ttgatagtga ccttcctcac cctggataga gatagaggct
     2461 tggtcagact gagtgacagc cacttccaca tctggtagag aaatatgacc ttttaaaacc
     2521 tcaatagctt gcgtttgttt gggactaagt cctgtaaatc ttaccattgc ttttcctcct
     2581 gtagccagtt gacaagagca ccgtagagat tggcatctgc gtgataggtg caggcctgga
     2641 taactggtgc gaccgtgtat tcttcgtagg tatcgacaaa gttatcaaca gccttcttga
     2701 catcttggat aaaatctgga ttttgactga tagagccacc cagactgatg acatctggat
     2761 cgatcagata ctggatattg agcaagcctt gcgccagatt gcggttcatg cgctcaatgg
     2821 cttcttgaca aaggatatta ccagctgcgg cctcttggta aatcttgcga ccgtcccaat
     2881 cagtatgacc agatttttca atcacgtatc gtaccatatt cccagttgat gctagttgcg
     2941 accagttatt aagtttttca gcaggggcaa gggttgtcat gtagccaaat tctccaccca
     3001 agccgtggcg acctcgatga agtctaccat tgataatcat ggctccgcca atccctgtcc
     3061 caatcacgac acaggctgca ttttcaagat ctggatgagc tagtagttca ctgagtccaa
     3121 cgcagttggc atcattttct aaatggacag gtagctgata agagctaagc gcctcatacc
     3181 aagaaaagcc atggatgtag ggcaccgcac tgaagccatc aatcacacct gtctcttgat
     3241 tgactgcacc tggaacgctc atagcaatcc cactgtaatc ctgttctgac aagcgttgat
     3301 ctagccacgc tagtaaatcc tccaagtttt caggcgttga aatacttgtc ttatccagta
     3361 ttttcccatc aggagtcaga ctggcaaact taatcccagt ccctccgata tcaatcgttg
     3421 caatggtcat tgtttttacc ataaaaaggg gcgaatcagc agttagttct tactttttcg
     3481 cccctccttt cttttgatgt ttactttttg aaattaaatt tcttcttttt tgatgaattc
     3541 tgtacgaatc tcttgtggat ccaatggtcc tgaatgaact ggatatggtc gctcaagtaa
     3601 gtcaagaatg gtttgttgat gttctgatat gtgcacattt tcttgactca tattatagta
     3661 acggaagaca tagccttctt cattttccgc cactttaaag gctgttggac aaacttgcgg
     3721 tatgctgaga acagaatggc tcaagaggct accagtcgca accacgcttc cttcctgtct
     3781 agcaagctga aggctggtaa acggtgtctg caaggctttg gcacgacgga aagctgaaaa
     3841 gcgttcttgg gcttggtggc attcaagtgc aaactcgact tcaaactcac gcaagcattg
     3901 tgcttctggc gttgggaagt agccccagtc acctagctca cctgatgcac gcaaaatggt
     3961 cacggcaatg gtgttatccc caaggatttc gtattcattc aatcccttat tggatacagt
     4021 cacccctttt tcatcgtcat acagactgac aaaagcttgt tggtgttgag gattttcagg
     4081 attttcccat gaagcagctg gtttgtttgg tcgtgtcacc acctcataga tactttcaga
     4141 atcattgctt ggacgcgtgt tatgagtctt gaccaagaga cggatacggt gatccttggc
     4201 agtgttagta aagcgagtct tgaagcggat ttgtggattg tcaacaaaga caatcaactc
     4261 agtttccaga ggaatgtttg tcaattcttc tgaccgtcca gcctcacgct tcataaactc
     4321 gatgatacct tgttgctctt cttctagctt ttcatccgca ctgacaggca cggtcaattc
     4381 atgtttgagc aagattttag catagcgatc tgtgttttcc aagacctcgt ggcccttaag
     4441 ctctgcaaag attggctctg ttccttttgg ttggaaatag atatactcgt ttccgatgtc
     4501 cccacggtct tcgaagcgga taaagtcttc ataggcttcg tgagttgtct tgtcatatac
     4561 tgtgatattg tcatccacac tcaccgttac gaatggtgta tcaatcactc cgttttggta
     4621 aataccctca cggtgttctt gttttccttc cagcaattgg aaggttgtcc aagaaagcgg
     4681 cgctaggtga actggaatgg tcacgcgcac ttgtcgagca atacgagctt ggcggaactt
     4741 gtcttttggt aaatcgtact caaaattagc tccgaggtct tcgattgtag cctctacagg
     4801 acgaccatcc aagtcctcca cacggtaact tggtaagata agagcagcca tctttttgta
     4861 gccttctgtt gggtgcaatt ccttgaaatc acaagtcgcc acatcaatca ctgtgctgac
     4921 agtatcgacc ttatcatgca agcctgtgtt aatgacagta aagagatagt cactttgagc
     4981 cttatccgta gcaattttac ccttccactc gttgagcaag ttacttttaa caaagtttcc
     5041 tacttggttg accttggcaa aacgcgtttc catctcgcgg tgaacttcgt ccacgctaca
     5101 gccacagata ctatcatgtg gcgcattctg caaaagtgtt ttccaagcat aggtcaactg
     5161 gtccttgtgg ttgtgtccac cagtgataat agtcaagggt tctaccactt gctctaggag
     5221 gttgctattt tcttggaagg cttgttttag gtaaatgcgg gatgaagaag tgttggcaag
     5281 tgtgtaccag ccatctgttt cctgactggt caactcgcct gtaacagttg ataagtgttc
     5341 aggaagcgca ccttctacag cttgaacata ttcatcaaaa gaactatgaa caaagattac
     5401 atccgggaag agttcatttg ccacacgaat ggcttcgctc agatttttct gtacaggctg
     5461 gtggtcacag ccgttcatca tcaaccattg gttggtcgaa gcgtaggcac gcacatctga
     5521 caatttttgt ttccagaagg tcaaggcctc atctttgtca actgggattt cattcccatt
     5581 actgtaccag ttggcaaaga gaatacctaa aacacgacta ccatccacac cctgccagta
     5641 catttctgaa aactgggatg taaaccgctc atcttcaagg acttggttgt caaatccaat
     5701 cggcttcaca ccacgaccaa aggctgccac gtgaatacct gatttttgaa ggatttgagg
     5761 agcttgtccc atatttccaa aggtatctgg aaagtagcca atctgggttg atttacccca
     5821 tttggcagct tcttgttgac caatcaaggt attgcggaca ttggcttcac tggagatcaa
     5881 gtagtcatcc tgcaagatgt aaaagggacc aattttaagt ttgccctcgt caatgtagcg
     5941 ttggaccttg tcgcgatttt cagggcgaat ttgtaagtag tcatcaagga caatagtttg
     6001 tccatccaag tggaaactct tgaactcagg gtcattttca aagagatcaa agagattgtc
     6061 aaacaattcc accaactgca tacgatggct ttcaaaaggc aagtaccact cacgatccca
     6121 gtgactatgt gagataatat gtacaacaac attttccatg aagtaaaacc tcattctaaa
     6181 tttaaatttt aacgttttaa atgtaaactt gtgattctga taagaatcgg ttgagctaaa
     6241 gcgagcccct tagcggatat ccaagtaatc caagaccaac tcacagaaca tcatgttagc
     6301 ccaggagaac cattcacgag agtagagggt cggatcatct acgtggaagc tttcgtgcat
     6361 gacacctgta ccaccatcgc aggcaaccag ctgatccagc aagaattttt tctctgcctt
     6421 atctcttgtt gtcaatcctt ggatagaaag ggcgattggc cagatatagc gatagaaggt
     6481 atgagaactt ccgagaccgc tagcgtattc tccttggtag aagtatggat tttcagagct
     6541 cagaatggta cggcgagtgg cttgatacac ttcatcatcg atcgaacagt agcccagata
     6601 gggcgcagct agtagacttg gtacatttgg atcatccatg atgctggcat ttcctaggcc
     6661 atctacttca aaagcgtaaa tcttttcgcc cttgctgttg gtggtgtaag cgtagttttc
     6721 gattccttct tggatttcat cctgaagacg cttggcatca gcaataacac tctggctatc
     6781 agctaggttt aatgctgcga agatttcttg cacataaccc aagactacta cagcaaacat
     6841 atttgacgga atcaagtaac tatactggca acagtcatca ctcggacgaa aagctgacca
     6901 agtcatacct gtcactgcaa agtcaggtcc aaagccatca tttaccaagg tgtcttcctt
     6961 acggtccgta tctcggacaa aacgataagg agagttcttg tggtcttgtt caactgtcca
     7021 tagatggaga atttccttag tcgctgcgac aaaagtttca tcaaactgac tagtctcgcc
     7081 agtctctttc cagaggagat aagccaactg caaaggatag caaagcgaat ccacctcata
     7141 cttgcgctcc cagatccagc cgttaaggtc tgtgtggtca gtctcgtggt gccctttcca
     7201 gttctcctca atgttgaagg agttagcata gggatccttg agtaccaagg tcatctgacg
     7261 tttgaccaaa cctgcaatgg tctgacgcag gagggcatct cttttagcta catgaaggta
     7321 gggtctgagt tgggctgtcg aatctcgaag ccacatggca ggaatatccc cagtcaagac
     7381 aaaagttgaa ccatcttcta agatttcaac cgtattgtcc aaggtatcgg tgtagcaacg
     7441 ctcaaagaca tcaacccact ctggatggtc cttggcccgc tctgctactt catctagcca
     7501 ttctctaaca atttctttcg aataaatcat cgtgcaattc cctctttcaa acaagtttca
     7561 tgttcagtat atagcttttg agacagaaaa tacatacaca aatgatagtc atttttctac
     7621 aaaatagtta tctttaaaac tccttttcta aaggccctct ttttctgata taatgtagaa
     7681 aaacagctaa aggaaagact atgaaaccac tacttgaaac catcgatacc cgctttggta
     7741 ctgctagcaa gcatgccttc tctcggggaa ataccctgcc atacacaggc gtgccttttg
     7801 ggatgaatta ctttgtgccc cagaccagtg accaggatgg atcttggttt tttgatccgc
     7861 atctgcctat ctttcagggg attcgattaa ctcaccagcc cagtccttgg attggggact
     7921 actcttggct ccttctgaca cctgtcacca gtcaactggg gggagacagc ctctttcacc
     7981 gccagtcttc ttatgatata gataaagctt gcttccagcc tcactatttg aagctctttt
     8041 ctctgcgtta tcagattgaa acccagctca caccgacttg ctatggtgct tctattcgtt
     8101 tgaatcaaaa gcaaggcaaa gccctctccc tctatcttca cgcagcagat gaactgacag
     8161 tagagcaagt agataagcgg actcttgccc tgcgacaaga aggtaaaact gaaactaaca
     8221 aaaattcgct aacgatgttc actgccctgc aaatgaacac ggatattctt gctatcagcc
     8281 aagaagctgg ggactggcga attgacttag caagtagtca aaccgagatg caactagcca
     8341 cttctttcat ctctccttct caagctctaa tcaatctacc tcaagaagat tttgatagct
     8401 gtaaaacaag tgcccaagcg gattgggaaa atctcctcca tcgttttgac attatagaga
     8461 taggagaggc tgaccgaacc ttctttgacc actgcctcta cagactcttc ctcttcccac
     8521 agacttttta tgagattaat gaatcagggc aagccatcca catggatctg gctactggta
     8581 ctgtcaagcc tggtgtcctc tttagcaaca atggtttctg ggataccttc cgcaccacct
     8641 tccccctctt tgcccttatc ataccggagc actatcaacg ctttttagaa ggtttcctca
     8701 atagctaccg cgatactggt ttccttccaa aatggctggc tccagatgaa cggggtatga
     8761 tgccaggtac acttttagac ggtattatcg cagatagcgc ctgcaaggac atggcccctg
     8821 acctagaagg agaactcttc caagccatgc tcgaaacagc cagcaaagcc gaccctctcg
     8881 gcatcaatgg ccgccacgga ctagcccaat accaagaact aggttacctc tctaccgacc
     8941 accacgaaag tgttagccat actctagact atgcctatag tgacttttgt atcgccagct
     9001 gtgccaaaaa actagagaac atagaaatcg ctgaaaccta caaggctact tcacaaaatt
     9061 accgccagct atttgacact gagacaggtt acatgcgagc acgagataat caaggaaact
     9121 ttcaccctga cttctctcct tatagttggg ggcgagacta cgctgaatgc tctgccattc
     9181 aagctacttt aggcgttctc cacgacatcc ctggcttaat ccaactgatg ggtggaaaag
     9241 aaacctttag caactatctt ttgaaagtct gtcaagatgc tcccctcttt gaaacaacag
     9301 gctatggtta cgaaattcac gaaatgagcg agatggctac tgctcctttt ggacaaatag
     9361 ccatttccaa ccagcctagt ttccacattc cttatctctt ccgctacagc gattaccctg
     9421 actacactgc ccttcttatc aagacgctcc gtcagaaagc ttttcaccca agttgggaag
     9481 cctatcctgg agatgaagac aatggtagtc tctctgcttg gtacatctgg tcagctctcg
     9541 gattttatcc aacctgtcca ggaaaaccaa gctatgacct cggaatccct ctctttgacc
     9601 atctacgagt ctacctagct aaagaagata aatggctgga tatccatact aaacaaaacc
     9661 acagccattt taactttgtc aaagaatgcc gactggacaa aacactcgta tcaactattc
     9721 aacaccaaga cctcctaaaa gctgaacaac taactttcac cctcagctgg ctaccaaatc
     9781 actaataacc aaaagaacct ttgcatcgcg caaaggttct tcttttatga aacttggact
     9841 tgaagttgat ccacaagctg accatccacc gttaggttca gatagaaatc caaggtttta
     9901 tctcctgcga aatacagggt tggtagatgc aaatcttttt tgctttcccc tgcttggaat
     9961 tgaaggactt gaatctcatc ctgataggcg acaccatgga cacctgtccc cggttgaatc
    10021 gaaatcttag cctctaaaga ggagctactt tcactacgtt caatcctaaa atgaagagtc
    10081 tctccctttg ccacagctag gcttttttct gcaaatgcta gttcctgaac aacttctttt
    10141 tttgataacc caggagtttt ataaagggaa atcttggtca gtagaggcat agattgtgct
    10201 tcggtaatca tcacgcgcac cttctgtgcc tctactagcg gacctcgcaa gaggcgctta
    10261 tgaccaactg tgaagcccct gccaaattcc tgccagacac catccacctc tacttgcaca
    10321 tggaaagcag cgattcgttg ccctagcttc aaatcttctc ttaactcaat tacatcaaaa
    10381 gttttaggag aacctaagtc gagttctaac tggatgggca agtctgcatc gcttgcccaa
    10441 gagctggtct caaggccgtc tgtcaaatgg cgacaagcaa agtctgcgga aagagctgga
    10501 ccagatacct cagctcccag agccaaatct tctttataga gctcattgcg ataggtcgca
    10561 aattcataaa gtcgttcaat atcctttgca tcaaagagcc cagcttgatt cggcggaata
    10621 ttaagcaaga gtggagttcc tcgccctact gagtgaaagt agatttcgac caactcctcg
    10681 agagacttag gatcctgatc ctcatggtag aaccagcctg gacggatgga aacatctgcc
    10741 tctccgattg aaaaaatcgt gcccgaggga tccccgtgct gaagatagtt cagctctgct
    10801 tctgttccta gtttatcagg attcaccttt tgccacagtg gatcacctgc ataccctcgt
    10861 tcattgccaa tccagcggat actggtgcct tctgttgaaa aaatcaagca agcgccctgc
    10921 aggtcacgaa tggtttcaaa ccatttttca aattcataat taaccttttg cgcgccctct
    10981 cctctggcac catccatcca aacctcagcg aacttaccag cattcccata gttaggattt
    11041 gataagattt ccttcaactg agccagataa taggcattgt agtccgcttc tcggtccaca
    11101 tgatagaggg gactatgggc atcccacggt gacaggtaga cccccatatc catatcaaac
    11161 tctgtggcag cttgggatac ttcaaggagc aagtcgccct ttcctctcct ccaaggactg
    11221 accttaaccg aataatctgt gtgagctgtc ggataaagga caaagccatc gtggtgcttg
    11281 accaccaaaa tcaacttttt gaagcccgtt tccttgagca cacgaaccca ctcacgcgca
    11341 tccaactgac tcgggttaaa gcgctcagga tcctcctgtc cagtccccca ttcttggtca
    11401 taaaaggtat taggaccgaa gtggataaaa gctgctagtt catctcccag ataagctagc
    11461 tgagtctgac ttggtaacgg tccatgcggt ttgattttct tcatcgttat atatctcctt
    11521 ctttagcaac ttctcttgtt cacaaggtta tccacaggtt gtggataata aaccatgatc
    11581 taacagccag ctctttccaa ctccaactgg taggctcagg atttttctcc tcaatattcc
    11641 ctcttcaaac agctctgtca tatggttctg ctaacacctc tcagaatgct actctccata
    11701 attattctca ggcttctttc aggtcattgt gtgaagacca ttcactctcc agacttttcc
    11761 acagattgtg aataagtgat tattctaaac tatcacctcg tcgactataa caaaaaataa
    11821 aaagagggtc aataagataa ttatctcttc ctaaatactc agaaaagatt ctataatttg
    11881 tacaattgaa caagaaatgc ttctccaaaa tctatcttca aaagaatgtt gcatcttttt
    11941 atcctttcac acaaacattt ctctttatac agatccaagt gataaccaga tgataattgg
    12001 acactttttg gttatttgtg tattttttga aaaatttttt aaaaaaattt tatatcctct
    12061 ttaatagaag tttatgcatt tgtatatatg tattccatcc tatgttgtgt ataataacat
    12121 aaaattgtgc taaatatcat aatttatatt attttccgag aaattatagc cattgacatt
    12181 acctaggaat tgcgttataa catacatgta agcggtaccc aaaatggtgc caagtcaaaa
    12241 tttttaagga ggaaaataca tgtcttcaca tccaattcag gtcttctcag aaattgggaa
    12301 actgaaaaaa gttatgttgc accgtccagg caaggagtta gaaaacttgt tgccggacta
    12361 tcttgaaagg cttctttttg atgatattcc tttcttggaa gatgctcaaa aagaacatga
    12421 tgcatttgcc caagctcttc gcgatgaagg aattgaggtt ctctacctag aacaactcgc
    12481 tgctgaatca ttgacctctc cagaaatccg cgatcaattt atcgaggaat acttagacga
    12541 agccaacatc cgtgatcgtc aaaccaaggt tgctattcgt gaattgcttc acggcatcaa
    12601 ggacaaccaa gaattggttg aaaaaacaat ggctgggatt caaaaagttg aattgccaga
    12661 aattcctgac gaagctaaag atctaactga cttagttgaa tcagattatc catttgcaat
    12721 tgacccgatg ccaaacctct atttcactcg cgacccattt gcaacaattg gaaacgccgt
    12781 atcgcttaac cacatgtttg cagacactcg taaccgtgaa acactctacg gtaagtatat
    12841 cttcaaatac cacccaatct atggcggaaa agtggatttg gtctacaacc gtgaagaaga
    12901 tacgcgtatc gaaggtggag acgagctagt tctttctaaa gacgtccttg cagtaggtat
    12961 ctctcaacgt acagacgcag cttctatcga aaaacttttg gtcaacatct tcaagaaaaa
    13021 tgttggcttc aagaaagttt tggcctttga atttgctaac aaccgtaaat tcatgcactt
    13081 ggatactgtc ttcactatgg tagactatga caagttcact attcacccag aaatcgaagg
    13141 cgaccttcac gtttactcag ttacttacga aaacgaaaaa cttaaaatcg ttgaagagaa
    13201 aggtgactta gctgaacttc ttgctcaaaa ccttggtgta gaaaaagttc atttgattcg
    13261 ttgcggtggt ggcaatatcg tagcagctgc gcgtgaacaa tggaacgacg gttctaacac
    13321 tttgaccatc gcacctggtg tggtagttgt ttatgaccgc aataccgtga ccaataagat
    13381 tttggaagaa tacgggcttc gcttgattaa gattcgcgga agtgaattgg ttcggggccg
    13441 tggtggacct cgttgtatgt ctatgccatt tgaacgtgaa gaagtgtaat cgctgttcgg
    13501 cattcgtcaa tagaaaatgt aaaaaataga aagaggaaat aataaaatga caaattcagt
    13561 attccaagga cgcagcttct tagcagaaaa agactttacc cgtgcagagt tagaatacct
    13621 tattggtctt tcagctcact tgaaagattt gaaaaaacgc aatattcaac accactacct
    13681 tgctggcaag aatatcgctc tcctatttga aaaaacatct actcgtactc gtgcagcctt
    13741 tacaactgcg gctatcgacc ttggtgctca cccagaatac ctcggagcaa atgatattca
    13801 gttgggtaaa aaagaatcta ctgaagatac tgctaaagta ttgggacgta tgtttgacgg
    13861 gattgaattc cgcggattca gccaacgtat ggttgaagaa ttggcagaat tctcaggcgt
    13921 tccagtatgg aacggtctaa ctgacgaatg gcacccaact caaatgctcg ctgactactt
    13981 gactgttcaa gaaaacttcg gtcgcttgga aggcttgaca ttggtatact gtggtgatgg
    14041 acgtaacaac gttgccaaca gcttgctcgt aacaggtgct atccttggtg tcaatgttca
    14101 catcttctca ccaaaagaac tcttcccaga aaaagaaatc gttgaattgg cagaaggatt
    14161 tgctaaagaa agtggcgcac atgttctcat cactgaagat gctgatgaag cagttaaaga
    14221 tgcagacgtt ctttacacag acgtttgggt atcaatgggt gaagaagaca aattcgcaga
    14281 acgtgtagct cttcttaaac cttaccaagt caatatggac ttagttaaaa aagcaggcaa
    14341 tgaaaacttg atcttcctac actgcttgcc agcattccac gatactcaca ctgtttatgg
    14401 taaagacgtt gctgaaaaat ttggtgtaga agaaatggaa gtaacagacg aagtcttccg
    14461 cagcaagtac gctcgccact tcgatcaagc agaaaaccgt atgcacacta tcaaagctgt
    14521 tatggctgct acacttggta acctttatat tcctaaagta taattttaga taataaaccg
    14581 tctaccaaca gctatgaggg ctgcgactaa tagctttagt ccggtcctct tttatgtaat
    14641 ggtaatctat tatttcttat aaaatatgtg aaaaatcatt aaattgaaat ctaaacgcat
    14701 tctattgagt gtgataaagg agaatttatg gcaaatcgta aaattgtagt agctttggga
    14761 ggaaatgcga ttctttcttc tgacccatca gcaaaggctc aacaagaagc tttagttgaa
    14821 acagctaagc atcttgtaaa attgataaaa aatggagatg atctgattat cactcacggt
    14881 aatggacctc aagttgggaa tctcttgctc caacatttgg catcagactc tgaaaagaac
    14941 cctgccttcc cactcgactc acttgtcgct atgacagaag gtagcatcgg tttctggttg
    15001 aaaaatgctt tgcaaaatgc tctcttggat gaaggcatcg aaaaaaatgt tgcctctgtt
    15061 gtaacgcaag ttgtcgtaga taaaaatgat ccagcttttg ttaacttgag taaaccaatc
    15121 ggtcctttct attcagaaga agaagcaaaa gcagaagccg aaaagagcgg agcgactttc
    15181 aaggaagatg ctggccgtgg ctggcgtaag gtcgttgcct caccaaaacc tgttgacatc
    15241 aaagaaattg aaaccatccg tactctttta aataatggtc aagtcgtcgt agctgcaggt
    15301 ggtggcggta ttcccgtcgt caaagaaaac aatggacatt tgactggtgt cgaagcggtt
    15361 attgataaag acttcgcttc ccaacgtttg gcagaattgg ttgatgcaga cctcttcatc
    15421 gttttgacag gtgtagatta tgtatttgtt aactacaaca agccaaacca ggaaaaattg
    15481 gaacatgtga atgttgccca gctagaagaa tatatcaaac aagatcagtt tgcaccaggt
    15541 agcatgcttc caaaagtaga agcagctatc gcttttgtca atggtcgtcc agaaggaaaa
    15601 gcagttatta cttcccttga aaatctaggc gccttgattg aatctgaaag cggaacaatt
    15661 attgaaaaag gataagttgt tttactaata agatgtattc tatttctagt atctttatat
    15721 caaattagaa attattcttg aaaacatgta caatatttca aaagatacta gttttagact
    15781 ttaatatggt aaaacaaata taaatagaaa gcgttttctt aaatgtttat ttaagaaagt
    15841 agttggtttt ttacactttg ttagacatca ggaggaaaaa caaatgagtg aaaaagctaa
    15901 aaaagggttt aagatgcctt catcttacac cgtattattg ataatcattg ctattatggc
    15961 agtgctaact tggtttatcc ctgcgggggc ctttatagaa ggtatttacg agactcagcc
    16021 tcaaaatcca caagggattt gggatgtcct catggcaccg attcgggcta tgctaggtac
    16081 tcatccagag gaaggttcgc tcattaaaga aacgagcgca gcgattgatg tagccttctt
    16141 catccttatg gttggtggtt tccttggcat tgtcaacaaa actggtgctc ttgacgtagg
    16201 gattgcctct atcgtgaaga agtataaggg ccgcgaaaaa atgttaattt tggtactgat
    16261 gcctttgttt gccctcggtg gtacaactta tggtatgggt gaagaaacaa tggccttcta
    16321 tccactcctt gtgccagtta tgatggccgt tggttttgat agcctgactg gtgttgcaat
    16381 tattttgctc ggttctcaaa tcggctgttt ggcatctact ctgaatccat ttgcgacagg
    16441 tattgcttca gcgactgcgg gagttggtac aggggacggt atcgtacttc gtctgatctt
    16501 ctgggttacc ttgactgctc ttagtacttg gtttgtttac cgttatgcgg ataagattca
    16561 aaaagatccg actaagtcac tggtttatag tactcgcaaa gaagatttga aacactttaa
    16621 cgtagaagaa tcttcatctg tagaatctac acttagcagc aaacaaaaat cagttctctt
    16681 cttatttgtg ttgacattca tcttgatggt attgagcttc attccatgga cagaccttgg
    16741 cgttaccatt tttgatgact ttaatgcttg gttgactggt cttccagtta ttggtaatat
    16801 tgtcggttca tctacttctg cactaggtac ttggtacttc ccagaaggcg caatgctctt
    16861 tgcctttatg ggtatcctga ttggtgttat ttatggtctt aaagaagata agattatctc
    16921 ttccttcatg aatggtgctg ctgacttgct cagtgttgcc ttgatcgtag cgattgctcg
    16981 tggtattcaa gttatcatga acgacggtat gattaccgat acaatcctca actggggtaa
    17041 agaaggcttg agcggtctat cttcacaagt ctttatcgtt gtaacttata tcttctatct
    17101 acctatgtca ttcttgatcc catcttcatc tggtcttgcc agcgcaacta tgggtatcat
    17161 ggctccactt ggagaatttg taaatgtccg tcctagcttg attatcactg cttaccaatc
    17221 tgcttcaggt gtcttgaact tgattgcacc aacatctggt attgtgatgg gagctcttgc
    17281 acttggacgt atcaacattg gtacttggtg gaaattcatg ggcaaactcg tagtcgctat
    17341 tattgtagtg accatcgccc ttcttctcct tggaaccttc cttccattcc tataaaatag
    17401 tgagtgaggt gattccatga aaatagatat aacaaatcaa gttaaagatg aatttcttat
    17461 atcattaaaa accttgattt cctatccttc agtactcaat gaaggagaaa atggaacacc
    17521 ttttggacaa gcaatccaag atgtcctaga aaaaacttta gagatttgtc gagacatagg
    17581 tttcactacc tatcttgacc ctaaaggtta ttacggatat gcagaaatcg gtcagggagc
    17641 agagcttctg gccattctct gtcatttgga tgttgttcca tcaggtgatg aagcagattg
    17701 gcagacaccg ccatttgaag caactatcaa agacggctgg gtattcggac gtggtgtcca
    17761 agatgataaa ggcccttcgc tcgcagctct ctatgcagta aaaagcttgc tggaccaagg
    17821 tattcagttc aaaaagtgcg tacgctttat ctttggtacc gatgaggaaa ccctctggcg
    17881 ctgcatggca cgctacaata ccatcgaaga acaggccagt atgggctttg cacctgactc
    17941 atcttttcct ctgacctatg ctgaaaaagg gcttctacaa gtcaaacttc atggccctgg
    18001 atcagatcaa ctagagcttg aagtaggagg cgcctttaac gttgtaccag acaaggccaa
    18061 ctaccaaggt cccctctatg aacaggtttg taacgatctc aaagaagctg gttatgatta
    18121 ccaatccact gaacaaaccg taacggttct cggagtgcca aagcatgcta aggatgctag
    18181 tcaaggtatc aatgctgtca tccgactagc taccattctt gctcctctcc aagaacaccc
    18241 tgctctcagc tttcttgcaa cacaagcagg tcaagacggc acaggaagac aaatctttgg
    18301 tgatatagca gatgaacctt ctggtcacct atcctttaat gtcgcaggtc tcatgatcaa
    18361 tcatgaacgt tctgaaatcc gtattgacat tcggactcct gtcttagctg ataaggaaga
    18421 actagtagag ttgcttacaa gatgtgcaca aaactaccaa ctccgctacg aagagtttga
    18481 ctatctagcg cctctatacg tcgcaaaaga cagtaaactc gttagcacac tgatgcaaat
    18541 ctaccaagaa aagactggcg ataacagtcc tgctatttca tccggtggtg ccacttttgc
    18601 tcgcaccatg ccaaattgtg tagccttcgg cgccttattc ccaggagcga agcagacaga
    18661 acatcaggca aatgaatgtg ccgttctaga agatttgtac cgtgctatgg atatttatgc
    18721 cgaagccgtc tatcgacttg caacttaatc aggcaactgt ttctaccaaa gaaaatcgac
    18781 cgattaatga actgcacccc aaaagttaga cagaataaat ctaacttttg gggtgtttta
    18841 ttatgaaatt gagttatgaa gataaagttc agat
//
LOCUS       DAVBCG010000027        18692 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBCG010000027 DAVBCG010000000
VERSION     DAVBCG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.027"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            2..589
                     /locus_tag="VQ495_000777"
     CDS             2..589
                     /locus_tag="VQ495_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2530761.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCSDELTILQILYGASLIS
                     FLWMYGQRKQVIKVNMKTRIQWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            989..1798
                     /locus_tag="VQ495_000778"
     CDS             989..1798
                     /locus_tag="VQ495_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2530762.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            1803..2504
                     /locus_tag="VQ495_000779"
     CDS             1803..2504
                     /locus_tag="VQ495_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2530763.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            2529..3446
                     /locus_tag="VQ495_000780"
     CDS             2529..3446
                     /locus_tag="VQ495_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2530764.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            3474..5015
                     /locus_tag="VQ495_000781"
     CDS             3474..5015
                     /locus_tag="VQ495_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET2530765.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            5034..5492
                     /locus_tag="VQ495_000782"
     CDS             5034..5492
                     /locus_tag="VQ495_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2530766.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            5494..7716
                     /locus_tag="VQ495_000783"
     CDS             5494..7716
                     /locus_tag="VQ495_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET2530767.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            7756..8865
                     /locus_tag="VQ495_000784"
     CDS             7756..8865
                     /locus_tag="VQ495_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2530768.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            9022..9912
                     /locus_tag="VQ495_000785"
     CDS             9022..9912
                     /locus_tag="VQ495_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2530769.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            9937..10260
                     /locus_tag="VQ495_000786"
     CDS             9937..10260
                     /locus_tag="VQ495_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530770.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            10250..12241
                     /locus_tag="VQ495_000787"
     CDS             10250..12241
                     /locus_tag="VQ495_000787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2530771.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQSNNEEIERLIKW
                     EKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKNNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETRILYFIKVMDERFGHSIYIYE
                     SETLSDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSETKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMIILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFVWCVILLGLLF
                     IAVGMLMPDMRLLFVLGKWVSIFNAVGILVVSIIQAKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGTLAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            12257..12733
                     /locus_tag="VQ495_000788"
     CDS             12257..12733
                     /locus_tag="VQ495_000788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET2530772.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            12767..13348
                     /locus_tag="VQ495_000789"
     CDS             12767..13348
                     /locus_tag="VQ495_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530773.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            13413..14420
                     /locus_tag="VQ495_000790"
     CDS             13413..14420
                     /locus_tag="VQ495_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET2530774.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            14410..14730
                     /locus_tag="VQ495_000791"
     CDS             14410..14730
                     /locus_tag="VQ495_000791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET2530775.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            14793..16568
                     /locus_tag="VQ495_000792"
     CDS             14793..16568
                     /locus_tag="VQ495_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET2530776.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            16569..17954
                     /locus_tag="VQ495_000793"
     CDS             16569..17954
                     /locus_tag="VQ495_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET2530777.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            17977..18588
                     /locus_tag="VQ495_000794"
     CDS             17977..18588
                     /locus_tag="VQ495_000794"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET2530778.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         6310 a         2651 c         3706 g         6025 t
ORIGIN      
        1 tatgaaaaga ataattccag tttatatatt ccaacaagta aatgtcctat tggtatctct
       61 atacttattg aaatttcttt gtagcgatga gttaactata ctacagattc tctatggagc
      121 gtcactcatt tcttttttat ggatgtatgg tcaacgaaaa caagtgatca aggttaatat
      181 gaaaactagg atacaatggc ttggtattgg attcgttagc ctactgatta taagtctatg
      241 ttttagtcta atccatgcgc aagaaagcac gaatcaagca aacttaattg gacttcaaca
      301 tcaaattcct tggttttcat ttttattgtt tttaatcaat gcgagtatgg ttgaagaatt
      361 tctgtataga gaaattttat ggaacttggt tagaaaatta gatattcgag ttgctttgac
      421 aagtgtttta tttgccttag cacatcatcc aggaaccatt atagcttggt gtttgtatgt
      481 atcgctgggg atgtttttag ggcttgtacg ctacaaattg gacttatggg gcagtatggg
      541 gctacatttg gtgtggaacc tactagttta tagtttgctg cttttttaaa ataagtgatc
      601 tgatttatct cataaatcag tagtttttat aggattattt ttaattcgga aacaaaaatg
      661 tattaacagc ataaaataaa gaaactagga acattttttt tattttttag aaataaaatg
      721 ttgtatagaa attccgaaag aaataataaa atcaaaataa aaattaataa aataggtata
      781 tttgaacata ttgacgtcga caaatgttct tgcaagacat aaaatacaaa taaaaagaat
      841 aaaaaatttt tatattttta tatttgttgt tctgttataa aatgaaagaa attagaaact
      901 agtttctaaa tcatgaaaaa tattgaaaaa aaaaaaaata agtgttaaaa taaagaatgt
      961 aaacgtttac ttcaataaag gagctcatat gttactgcaa aaagaactaa ttccaatgat
     1021 agaagctaac ttaccaaata tggcatatgc tgaaaaagac attgctaaat tcttcttaaa
     1081 acagcaacct ctgaataatt attcatgtaa ggcattgtgc gaatgcctta atgtatccaa
     1141 agcaacattg actcgatttg cgaaaaaatg tggttttaaa ggttttagac aattcatttt
     1201 caaataccaa gagatgattc gtgagaaaga aaagttagca ttgtatacag aggctacaga
     1261 aaaagtttta tccgactatg aagaaatgtt gagaaaaact tacacggttc ttgatgaagt
     1321 tcaacttgag cgtattgctg agatgataga aactgctgag cgtgtatatc tttatggtaa
     1381 agtaagttct gttcttgctt tacaagaaat gaagatgaga tttatgcgtc tcggagtcat
     1441 tggtgaagta ttatcagacg aggatatgat tttgtggaat agcttattac ttaatgaaaa
     1501 ttgccttgtc attggagcat ccgtttcagg tcaaactgat attgtactag aaggtcttca
     1561 aaaagctgcg gataaaggcg ctaaaacagt tttaatgact acaagaaaat ttgacgaaga
     1621 agattgtttc tttgatgaac tattgttatt agcttcgacc aatcatctct cgtatggcaa
     1681 tcgcatatca cctcagtttc caatactttt aattacagac tgcttattct ctcattatct
     1741 ggaaagtcca gagagacaat attattacaa tcaaactatt atccataagg aggaataata
     1801 aaatgagtca aatttggact aaagaaaaat ttataagtca agtccatggt ggagtcattg
     1861 tttcttgtca agctttaccc ggtgaacccc tttataatga agaatttagc ttgatgcctt
     1921 ttatggctaa agcagcttta gaggcaggag cagtgggcat tcgcgcaaat tctgtgcgtg
     1981 atattaaagc aattcagaaa gtagtagatt taccaataat tggaattatc aaaagagatt
     2041 atccacctca agaaccatat attactgcta cgatgaaaga agtagatgaa cttgtagaat
     2101 gcggaacaac agtcattgca tttgatgcaa ctttaagacc aagatatgat ggcttagtta
     2161 tcaatgaatt tatcaaaaaa ataaaagaaa aatatcctaa tcaattgttg atggcggatg
     2221 taagtaattt tgatgaaggt ctctatgcat ttaaatcagg cgttgatttt gttggtacaa
     2281 cattatcagg gtacacaagt acaagtgtac aatcagatga gcctgatttt gaactaatga
     2341 aaaaattggc tgattttaat attccggtaa ttgccgaagg aaaaattcat tatccagaac
     2401 aattaaaaaa agcttatagt ttaggtgtta ccagtgttgt cattggtgga gcgattacac
     2461 gtccaaaaga aattgctcag cgatttatta atgtcatcaa ataaaaaaca atataaagga
     2521 gaaatctgat gaaagattta actaaataca aaggcgttat tcctgcattt tatgcttgct
     2581 atgatgaaaa tggtgaaatt agccaagaac gtgtaaaatc tctggtgcaa tatttcattg
     2641 acaaaggtgt aaaaggtatc tatgtaaatg gttcttcagg tgaatgtatt taccaaagtg
     2701 tagaagatcg taaacaaatt attgaagctg ttatggaagt tgctaaaggt aaattaacag
     2761 ttatcaacca tgttgcatgt aataacacga aagatagtat tgaattggca aaacattcag
     2821 aaagtgttgg agtcgatgct attgctgcta tcccacctat ttacttcaaa ttgccagaat
     2881 attcaattgc agcatattgg aatgcaatga gtgaagctgc gccaaataca gattttatca
     2941 tttataatat tccacaattg gcaggggttg cgttgactgg tagtttgtat gcaacaatgc
     3001 gtcaaaatcc tcgtgtgatt ggagttaaaa attcttctat gcctgtacaa gatattcaaa
     3061 tgtttgtagc tgcaggtgga gaagattaca ttgtattcaa tggtccagat gaacaatatc
     3121 ttggtggtcg cttgatggga gcagaagctg gtattggtgg tacttatggc gttatgccag
     3181 atttgttctt gaaattggaa agtttgattc aagaacgaga tttagatact gctaaaaaac
     3241 ttcaatatgc tatcaatgaa gttatctata agatgatatc aggtaaggca aatatgtatg
     3301 ctgtagcaaa agaggttttg cgtctaaatg aaaaacttga tttaggttct gttcgtcaac
     3361 ccttagaagc attggcagaa ggtgacttgg aagttgcaaa acaagcagca gaacttattc
     3421 aacaagcacg aaaagaattt ttataataaa taaaatcaat atggagatat gttatgggca
     3481 caacaggatt tacaataatt gacttaatta tcttgattgt ttatttactt gcggtgttgg
     3541 ttgcaggtat ctatttctct aaaaaagaga tgaaaggaaa agagttcttc aaaggagatg
     3601 gttcggttcc ttggtatgtt acttcggtat ccatttttgc cacaatgctc agtccgattt
     3661 ccttcttggg actcgctggt aactcttatg caggtagctg gattttatgg tttgctcaat
     3721 tagggatggt agtagctatt ccactgacaa ttcgttttat cttacctatc tttgcacgga
     3781 tagacatcga tacggcatat gattacttgg ataaacgttt taattctaaa gcacttcgta
     3841 ttatttcagc actcttgttt attatttatc aattgggacg tatgtctatc attatgtacc
     3901 tcccatcagc tggtttatca gtattgacag gaattgacat caatattttg attattttga
     3961 tgggtgcagt tgcaattgtt tattcttaca ctggtggtct aaaatccgta ttatggacag
     4021 actttattca aggtgtgatt ctgattagtg gtgtcgtttt agctttattt gtactgatgg
     4081 ctaatattaa aggtggcttt ggtgcagtag cagaaacatt agcaaacggg aaattccttg
     4141 ctgcaaatga aaaacttttc gatcctaact tgctttcaaa ctccatcttt ttaattgtga
     4201 tgggttcagg ctttacaatc ttgtcttcct atgcttcatc tcaagatttg gttcaacgtt
     4261 ttactacaac acaaaatatt aagaaactta ataagatgtt gttcacaaac ggtgtcttgt
     4321 cacttgcaac tgcaacagtc ttttacttga ttggtacagg cttgtacgta ttctatcaag
     4381 tacaaaatgc aggtagtgca gctagcaata tccctcaaga ccaaatcttt atgtacttta
     4441 ttgcatacca gttaccagta ggtatcacag gtttgatttt ggcagcgatt tatgcagcat
     4501 ctcaatcaac tatttcaaca ggtttgaact ctgttgcaac ttcatggaca ttggatattc
     4561 aagatgtcat ttctaaaaat atgtcagacg atcgtcgtac gaaaattgca caattcgtat
     4621 ctctagcagt aggtttattc tcaattggtg tttccattgt catggctcac tcagatatta
     4681 aatctgcata cgaatggttc aatagcttca tgggacttgt acttggtcta cttggtggtg
     4741 tatttattct tggatttgtt tctaaaaaag caaataaaca aggtgcttat gcagcgctga
     4801 ttgtatcaac tatcgtcatg gtatttatta aatacttcct tcctccaaca gctgtgagct
     4861 actgggcata ttcattgatt tcaatctctg tatcagtagt ttcaggttat attgtatctg
     4921 ttcttactgg aaataaagta tctgcaccta aatatacaac gattcatgat attccagaaa
     4981 ttaaagcgga ttcaagttgg gaagttcgtc actaatagaa aagagaaaat aatatgatta
     5041 tttctgaaca atctgatttt agacgatatg cttctatcaa taaacatttt tcaaaagtct
     5101 gtgatttttt agaaaataca aatttgacag atttagttga tggaaaagtt gatattgacg
     5161 gggaaaatgt ttttgcaaat tgtatgatct atctagctga tggagtccca ggagatattt
     5221 ttgaaactca taagaaatat ttagatattc atattgttgt tgaaaatact gaaaaaatgg
     5281 cagtaacttc cccagttcgt gcccagtcac gtgtaccttt tagcgaagaa aaggatattg
     5341 ctttttacga tagcaaagac tatcaaattg ttgaattgct tccagggaat atgttggtga
     5401 cttttgaaga agatcttcac caaccaaaga ttcattgcaa tgatgagcct gttaaaaaac
     5461 ttgttattaa agttttaaac gaggaaaaat aaaatgaaaa aaaatattaa acaatacgta
     5521 accttaggta ctgcagtggt attatcagca tttgttgcta actcagttgc agctcaggag
     5581 actgaaactt ctgaagtatc aacaccagag ttggtgcaac ctgttgcacc aacgactccg
     5641 atttcggaag tacaacctaa atcgggtaac tcttcggaag ttactgtaca acctagaaca
     5701 gtcgaaacta ctgttaagga tccatcttct gcaacggaag aaactcctgt cttagaaaaa
     5761 aataatgtta ctttaacagg gggcggagaa aatgttacta atgagttaaa ggataaattt
     5821 actagcggtg actttactgt agtgattaag tacaatcagt ctagtgagaa aggcttacaa
     5881 gctctgtttg gaatatctaa ttctaaaccc ggtcaacaaa atagttatgt agatgtgttc
     5941 cttagagaca atggtgaatt gggaatggaa gcgcgtgata cttcttccaa taaaaatcac
     6001 ctagtatcca gacctgcttc agtttggggt aagtacaaac aagaagctgt gactaacact
     6061 gttgcagtag tagcagattc agtcaaaaaa acatattctt tatatgcaaa tggtacaaaa
     6121 gtagtagaaa agaaagtgga taacttctta aacatcaagg atattaaagg tattgattac
     6181 tatatgcttg ggggagtgaa acgtgcagga aaaacggcgt ttggttttaa cggaacacta
     6241 gaaaatatca aattctttaa tagtgcattg gatgaagaaa ctgttaaaaa gatgacaaca
     6301 aacgctgtta ctggacattt aatttatacg gctaatgata caacaggttc taactatttc
     6361 cgtattccag ttctgtatac ttttagcaat ggtcgggtat tttcaagcat tgacgctcgt
     6421 tacggcggaa ctcatgattt tttgaataaa attaatattg ctacaagtta tagtgatgat
     6481 aatggtaaga catggactaa accaaaatta acattggcat tcgatgattt tgcgccagta
     6541 ccattagaat ggcctcgtga tgttggtgga cgtgacttac aaatcagcgg tggtgcaacc
     6601 tatattgact ctgttattgt tgaaaaaaat aacaaacaag tactcatgtt tgcggatgtg
     6661 atgcctgctg gagtaagttt tagagaagct actagaaaag attcaggtta taaacaaatt
     6721 gatggtaatt attaccttaa attaaagaaa caaggtgata ctgattacaa ttatactatt
     6781 cgtgagaatg gtactgtata cgacgatcgt accaacagac caactgaatt ttcagtagat
     6841 aaaaatttcg gtattaaaca aaatggtaat tatttgacag tagagcaata ttcggtttca
     6901 tttaaaaata ataaaaagac tgaatatcgt aatgggactc acgtccatat gaatatcttc
     6961 tacaaagatg cattgttcaa agtagtgcca acgaactata ttgcatatat ttctagcaat
     7021 gatcatggcg aatcttggtc tgcaccaact ttattacctc ctataatggg acttaatcgg
     7081 aatgcgcctt atttgggtcc tggacgtgga atcattgaaa gctcaactgg acgtattctt
     7141 attccgtctt acactggtaa agagtctgca ttcatttata gtgacgataa tggagcatct
     7201 tggaaagtta aagttgtgcc acttccttct agttggtcag cagaagcaca atttgtagaa
     7261 ttgagtccag gagtaattca agcatacatg cgtacaaata atggtaaaat tgcatattta
     7321 acaagtacag acgcaggtac tacttggagt gcaccggaat atttgaaatt tgtttcaaat
     7381 ccaagttatg gaacacaatt atcaatcatc aattatagtc aactgattga tggtaaaaag
     7441 gctgtcattt taagtactcc aaactccaca aatggtcgta aacacggaca aatttggatt
     7501 ggtctaatta atgatgataa tacaattgat tggcgttatc atcacgacgt tgattatagt
     7561 aactatggat actcatattc aacattgaca gagttaccaa atcatgaaat tggattgatg
     7621 tttgaaaaat ttgattcatg gtctcgtaat gaacttcata tgaaaaatgt tgtaccatat
     7681 ataacattta agattgaaga tctgaaaaag aattaaagct gaaatttgaa aatatataaa
     7741 aagaggataa aaattatggt aaattacggt attgttggag ctggatattt tggagctgat
     7801 ttagctcgct caatgaacaa aattgaagat gcaaaagtgg ttgcggtatt tgacccaaat
     7861 catggagaag aagttgctca agagttggga tcagatgttt gtgcaagttt agatgaactt
     7921 gtagcacgtg aagatattga ttgtgtgatc gtagcttcac ctagctacct tcaccgtgaa
     7981 ccagttgtga aagctgctca acatggcaaa cacgtatttt gtgaaaagcc aattgcattg
     8041 tcttatgaag attgtaaagc catggttgac gcatgtaaag aaaataatgt tatctttatg
     8101 gctggtcaca tcatgaactt ctttaacggt gtacaccatg ctaaagaatt gattactcaa
     8161 ggtaaaatcg gtaaagttct ttattgccat gccgctcgta caggttggga agaacaacaa
     8221 ccaactgtat catggaagaa acttcgttct caatctggag gacatttgta ccatcatatt
     8281 catgaattag attgcattca gtttatcatg ggaggacttc ctgaaaaagc gacaatggta
     8341 ggaggcaatg tatatcataa aggtgaaaac tttggtgatg aagatgatat gctcattgta
     8401 aacttagaat actctgatga tcgttatgct gttttggaat atggtaatgc tttccgttgg
     8461 ggtgaacact acgtcttgat tcaaggaact gaaggagcta tcaaacttga tttgttcaat
     8521 actggaggta ctcttcgtgt taaaggtgaa ggagaatcac acttcttagt tcatgaaact
     8581 caagaggaag atgatgaccg tacagctatc tataccggtc gtggtatgga tggagcaatt
     8641 gcgtacggta aaccaggagt acgttgccca ttatggttgc aaacatgtat tgataaagaa
     8701 atggaatatc tacatgacat cattaaaggt ggagaaatta cagaagaata tgaaaaactt
     8761 ctcaatggtg tagctgcttt agaatcaatc gctaccgctg atgcatgtac tttatcagtt
     8821 aaagaagatc gaaaagtaag tctttcagaa atcacaaatg cttaactttt gtaaaacaga
     8881 atagtaaatt cttatcatta tataatttct aaagttctgt gatacaactc attgaataaa
     8941 gaaatagaga tgggactggg ataatgccca gtcccatttt ttatcaaaaa gtaatgagat
     9001 caaaaatgtg ggagtgatga aatgaagatt atagggatcg atattggcgg aacaacaatt
     9061 aaagcagatt tatacaatga gtttggaacg agtttaaatc agttcaaaga gatagaaaca
     9121 attattgact atgatttggg aacgaaccag atattaaatc aggtctgtga tttaattggt
     9181 gagtatattt taaatcattc aattgatggt gttgggattt ccactgctgg agttgttaat
     9241 gctaatacag gagagatcat ctatacaggc tatacaatac cagggtatat cggagtaaac
     9301 tttactttcg aaatagaaaa acgttttggg ttgtctactt ttgttgaaaa tgatgttaat
     9361 tgtgctgcat taggtgaatt gtggaaggga caagccaaag ataagaaaaa tgtagtaatg
     9421 gttactattg gaacaggtat aggaggcagt attattgtca atggacaaat tgttaacgga
     9481 tttaactata ctgctggtga agtaggctat attcctgtag gtaattcgga ttggcaaagc
     9541 aaagcctcaa caaccgcatt gattcattta tatcaaaaga aaagcttgaa aactaatcaa
     9601 actgggcgta ctttctttac tgatttaaga gctggagata aagtcgctaa agaaactttt
     9661 gaaatttttg tagaaaatct aacaaaaggt ttattaacga ttacttatct acttaatcca
     9721 gaaattctca tattaggagg tgggatcctg gctaaaaagg atattttgtt acctgaaatt
     9781 caaagttctt tagctaaaaa tgcaatggat aataggtttt tgcctaaaaa tcttgtggca
     9841 gctacattag gaaatgaagc tggtcgtata ggagctgtaa aaaatttctt agatagaatt
     9901 tctaataaat agtatgaaag ataaggaggt gtcacaatga ctaactctgt attttcgaca
     9961 atgcaagata ttgagaatgt tgcaaccgat attataaaat catatgataa tgagatttat
    10021 acttataaag ctgtttccca agaagaattg gaaaaactag aaaaaagtta tgatgaaaaa
    10081 agtcacgaag aattagtttc aatagaaagc aatttagaaa tgaaacaaca gaaccttatt
    10141 gatgaggtta ataaaacaat caaggaaaat tatgaaaaga ttcagtatat ttcatcaagt
    10201 aggagaggag aatttgtaga aaaaattatt ggtagggtgg tagaaaaata tggctattag
    10261 tcagatgaaa agaatttctc tactattttc taaaagtagt cttgatgatg ttttaaaaac
    10321 tattcaagaa ctagagtcag tgcagttccg tgatttaaag gttcaggata actggtcaga
    10381 agctctagaa aaagatgagg ttgtatttcc aactattcaa atttctcata cttctaattc
    10441 caatcatggg gttattgaag gaaatgatgc cttgatttat ttgatgaatc aacaacaata
    10501 tttagaagca actgtagaga aattacaaga atacctaccg aaagaaaaca cgtttaaatt
    10561 attacagcaa cctccgataa cgacctctta tgaagaatta gaaaaatttg gtaaagctaa
    10621 tgttgctgag ggtgttctta aaaaagtgaa tcgtcaaata aacagagttc atgaattaga
    10681 aagacacatt caaagtaata atgaggaaat agagagatta ataaaatggg aaaaattaga
    10741 aattattcct gcgcatttag aacaattttt tttctgtaaa ggaaaagtcg gaacaattcc
    10801 aaggactgta gataatcgct tatacaatag ttttttagaa aacaatattg aagttcaaga
    10861 aatattttct aatgatagag agtacggtgt tgttgttttc tatcagtcca gttgctctat
    10921 tgattttgat gaatacttat ttgaaccatt tgattattct agaaaggaat taccgaagca
    10981 gcgagtagta gatttggatc aagaaaacat gcagttaata actgaaaaag agaatattat
    11041 cgcatcgttg caagattcaa agaataattt gatagattta caatggcaaa ttgactatat
    11101 tttatctatc tatgctcgtc aaatctctaa gaataacctt ttgagcactc cgcatctagt
    11161 tgcactagaa ggatggatag aagaaactcg tattttatat tttataaaag ttatggatga
    11221 gcgttttgga cattctattt atatttatga atcggaaaca ttgtcggata atcaagatga
    11281 aatacctatc aaattaacga atcattcttt aattgaacca tttgaattat tgacagaaat
    11341 gtatgctctg cccaaatatt atgagaaaga tcctacacct gtattagcac cattttactt
    11401 tacatttttt ggaatgatgg ttgctgattt aggctatggt ttactattgt ttttaggaac
    11461 aatgttagca ttaaaaattt ttcatctacc ttcagaaact aagagatttt taaaattctt
    11521 taatatatta ggggtagccg ttgcaatttg gggtggaatc tatggctcat tttttggata
    11581 tgagttgcca tttcatctga tatctacaac ctctgatgtc atgattatat tagtagtgtc
    11641 agttgtgttt gggtttatta cagtatttgc aggtttgtta gcttcaggac tacaaaaagt
    11701 aagaatgaag aaatatgcag aagcatataa ttcaggattt gtgtggtgtg ttattctgct
    11761 tggcttgtta tttattgctg tcggaatgtt gatgcctgat atgagactgt tatttgtatt
    11821 agggaaatgg gtatctattt ttaatgctgt gggaattttg gttgtttcta ttattcaagc
    11881 caaaagcttg tcaggtattg gagcaggatt gtttaatcta tataacattt catcttatat
    11941 aggtgattta gttagtttca ctcgattgat ggcattagga ttatctggag caagtatagc
    12001 atcagctttc aatttaattg ttggtttgtt tccgggaaca ttggctaaac taacaattgg
    12061 attagtatta ttcattcttt tacatgcgat caatattttt ctatcgttac tatcaggata
    12121 tgttcatgga gcacgtctga tatttgttga attttttggt aagttttatg agggtggagg
    12181 aaaaccattt caacctttga aggcttctga gaaatatatt aaggttatta caaagaatta
    12241 atggaggata tatataatgg aacatttagc aacttatttt tcaacctatg gaggagcttt
    12301 cttcgctgca ttgggaattg tattggcggt tggattaagt ggtatggggt ctgcttatgg
    12361 agttggtaag gctgggcaat ctgccgcagc tttactgaaa gaacagccag aaaagtttgc
    12421 ctctgctttg atattgcaat tactgcccgg aacacaagga ttatatggtt ttgttattgg
    12481 aattttgatt tggttgcaat taactccaga acttccttta gaaaaaggcg ttgcttattt
    12541 ctttgtagct cttccaattg ctattgtagg atacttttcg gctaagcatc aaggaaatgt
    12601 agcagtagcg ggaatgcaaa tcttggctaa aagaccaaaa gaattcatga agggagcaat
    12661 tttggctgcc atggtagaaa cctatgcaat tcttgccttt gtcgtatcat tcattttgac
    12721 ccttcgtgta taagaaatta atttgctatt caaaggaggt gtctaaatga gcaatttaga
    12781 aaacttacga gagtctgtta ttgaacaagc tcatgaaaaa gggcgtatga aattattgga
    12841 ttccaaaaag aagattgatg atgaatttga aatgcaaaag tcggtcattg taaagaaaaa
    12901 agaagtggaa catgaacgaa agttaaaaga attacaacag aaatatcaaa taatttttca
    12961 acaattaaaa aataaggaac gccaatcaac gttagtatca aaacagaaaa tattaaaaga
    13021 actttttcaa tctgctttaa tagaaatgga atcttggagt gcagataaag aaatggagtt
    13081 catctatcga attctggaac gatattcaca acaagaggtc atagtaactt ttggggagcg
    13141 gactttagct aaattcaatc tggaacaatt agagaaattg aaattctctt ttccaaatca
    13201 tttatttagt gaacagccta tctcaaatga atcaggctta cttatttcaa taggtaaaat
    13261 tgatgataac tatttgtata aaacattaat tggatcgatt tctaaggaag aaagttcaag
    13321 tatcgcaaat caaattttta tcaattaagg atgaaattgg ttaatccttc ttagaaattt
    13381 ggagtattcc aataaaatta gaaaggtatt ttatggatac taatcttttt tcaaaaataa
    13441 atacgacgat ttcggtaaaa gaaaacgatt ttattacaga agataaattt caaaaaatta
    13501 tacaatccaa agatacggag acaatggcat ttatcttaga agcaactccc tatcatttat
    13561 cgattgacgt tttagaaaat cctagtcaga cagagacttc gctaatgaca aaattagtca
    13621 atgattatag atgggcctat gctgaaagtc cgtctaataa aattgtgact ttatttgctt
    13681 tacgatatgt ttatcataat atcaaagttt tattaaaatc taaggcggca attaagaaag
    13741 atttttctaa attattaatt ccaataggga tttttgatat agaaagttta aaacatttag
    13801 tttcttcctt acattcagat acacttcctg attttatggt tcatgaagta gaatcaattt
    13861 ggaatgagta tgaaactttt aataatattc gtgtacttga tgtcggagct gatctagcat
    13921 attttaaaca tctgaaactt ttatctaatg agttagatga ggtgctgtct caggttattg
    13981 tcgcaatgat tgacttttat aatattatta ctgtaaaacg tggtttatct caaaataaga
    14041 gtcatgggga tattttacaa ttactttcag atgaaggaag tatttctgct aaagaattta
    14101 tatacattgt agaaaatcaa gaaatatttg tgtggttcaa taaaataaat ccaagcttag
    14161 attcaatctt ttcaacttat gaattgaaga tgcaggacgc aacaatttca tcttctgagt
    14221 tagaattttt atgtgattta ttattgtata aaattttaga tcaaggaagg tacaatgtag
    14281 atgggccgtt agtacttgct agatatttat tgggatgtga gtttgaagta aagaatctca
    14341 gaatgattat atcagctctt caaaatacaa ttccctttgg atcaataaaa gaaaggatac
    14401 gcccgcatta tggaagctaa taagtataaa attggcataa ttggtagccg tgatattatt
    14461 ttacccttta gcatgattgg gtttgatata tttcctgcct atcaagaaca agaagctata
    14521 aatactctaa gaaaattagc ccaatctgat tatggtgtca tttatatcac tgaagacatt
    14581 gcttcaatga tattagatac aattcgccat tatgattctc aagttgtgcc tgctattatt
    14641 ttattaccga ctcataaaca aggtttaaat ttaggattaa aacgtataga ggataatgtg
    14701 gagaaagcag taggacacaa tattttataa taatgtacaa aattgtctgt aatattattc
    14761 tataattttt ggacttagta aggagaataa ctttgactca agggaagatt ataaaagtat
    14821 cgggacctct agttattgca tcaggtatgc aggaggctaa tattcaagat atttgccgtg
    14881 taggtaagct agggttaatc ggtgaaatta ttgaaatgag aagagatcag gcatctatcc
    14941 aagtctatga ggaaacatct ggtcttggtc cgggagaacc tgttgttaca actggagaac
    15001 ctctctcggt tgaattagga ccaggattga tttctcaaat gtttgatggc atacaacgcc
    15061 cattagatcg atttaaattg gctactcata atgattttct agttcgtggg gtagaagttc
    15121 caagtttgga tagagatatt aagtggcatt ttgattccac tatagcaatt ggtcaaaaag
    15181 tgagtacggg tgatattcta ggaactgtca aggaaaccga ggtagttaat cataaaatta
    15241 tggttcctta tggagtatct ggcgaagtcg tttctatcgc atctggcgat tttacaattg
    15301 atgaagttgt atatgaaata aaaaaattgg acggtagttt ctataaagga acgcttatgc
    15361 aaaaatggcc tgttcgcaag gcgcgtcctg tttctaaacg tttaatccca gaagaaccat
    15421 taatcacagg tcaacgagtt attgatacat tctttccagt aaccaaaggg ggagcagcag
    15481 ctgttcctgg accgtttgga gcaggaaaga cagttgtaca acaccaagta gctaaatttt
    15541 ccaatgttga tattgttatt tatgtcggtt gtggagaacg tggaaatgaa atgacggatg
    15601 tactgaatga gtttcctgag ttgattgacc ctaataccgg acaatcaatt atgcaacgga
    15661 cagttctgat tgctaatact tcaaatatgc ctgttgctgc tcgtgaggct tcaatttata
    15721 caggaattac catggctgag tattttcgtg atatgggcta ctctgtcgcc attatggctg
    15781 attcgacttc acgttgggca gaagcactac gtgaaatgtc aggacgtcta gaagaaatgc
    15841 ctggtgatga gggttatcct gcttatctgg gaagtcgtat cgctgaatat tatgaaagag
    15901 cgggacgttc tcaggttcta gggattccag aacgtgaagg aactattact gctattggag
    15961 ctgtatcgcc acccggtgga gatatttcag aaccagttac tcaaaacact ttacggattg
    16021 tgaaagtttt ttgggggctt gatgctccgt tggcacagag acgtcatttt cctgcaatta
    16081 actggcttac atcttattca ctctataaag acagtgtggg aatttatata gatggtaaag
    16141 agaagacaga ttggaatagt aaaataactc gtgcgatgaa ctacttacaa cgggaatcta
    16201 gtttagagga aattgttcgt cttgttggaa ttgattctct gtctgataat gaacgactaa
    16261 cgatggaaat tgctaaacaa attcgagaag attatttgca acagaatgct tttgattcag
    16321 tagatacatt cacttcgttt gcaaaacaag aagcaatgct aagtaatatc ctaacttttg
    16381 ctgatcaggc aaatcatgct ttagagttag gttcttactt tacagagatt atggaaggta
    16441 ccgtggcagt tcgagaccgt atggcgcgaa gtaaatatgt ttcagaagat agattagatg
    16501 aaatcaaaat tatttcaaat gagattacac atcaaattca tttgatatta gaaacaggag
    16561 gtctataaat gagtgttata aaagaataca gaactgctag tgaagttgtt gggcctctta
    16621 tgattgttga acaagtaaat aatgtgtctt acaatgagtt agttgaaatt caacttcata
    16681 atggagaaat tcgtcgtgga caagttttag agattcacga agataaagca atggttcaac
    16741 tttttgaagg atctagtgga ataaatttag aaaagtctaa aattcgtttt gctggtcatg
    16801 cactagaatt ggctgtatct gaggatatgg ttggtcgtat ttttaatggg atgggaaaac
    16861 caattgatgg tggaccagat ttaattccag agaaatattt agatattgat ggtcaagcta
    16921 ttaatcctgt atctagagat tatccagatg aatttattca gacagggatc tcctctattg
    16981 atcatttgaa tactcttgta cgtggtcaaa aattaccagt attttcaggt tcgggcttac
    17041 ctcataatga attagcagct cagatagcaa gacaagcgac tgttttaaat tctgatgaaa
    17101 attttgcggt tgtatttgca gcaatgggta ttacttttga agaagctgag ttttttatgg
    17161 aagaactcag aaaaacagga gcgatcgatc gttcagtctt atttatgaac ttggcaaatg
    17221 atcctgcaat tgagcgtatt gcaactcccc gcattgcttt aaccgcggca gagtatctag
    17281 cttttgaaaa agatatgcac gttctagtta tcatgacgga tatgactaac tattgtgaag
    17341 cgttacgtga agtctcggca gcgcgccgtg aagttccagg tagacgaggc tatccgggat
    17401 atttatatac aaatttatca actctatatg aaagggctgg tcgcttagtt ggtaaaaaag
    17461 gttcagtgac acagattcct attttaacaa tgccagaaga tgacataaca catccaattc
    17521 ctgatttaac tggatacatt actgaagggc aaattatttt gtctcatgag ttgtataatc
    17581 aaggttatcg tccaccaatc aatgttttat cttctctctc tcgattaaaa gataagggat
    17641 ctggagaagg taaaactcgt ggagatcatg ctccaactat gaatcaactg tttgcagcct
    17701 atgcccaagg gaaaaaggtt gaagagttag cagtagtatt aggagaatcg gctttatctg
    17761 atgtagataa attgtatgtg agatttacaa agcgttttga agaagagtac atcaaccaag
    17821 gattttataa aaatcgaaat atagaagata cgttgaatct tgggtgggaa ttactatcaa
    17881 ttcttcctag aacagagtta aaacgtatca aagatgattt gcttgataaa tatttacctt
    17941 tggtagaagt ttaatccaga aatggagtga ttatctatgg tacgtttgaa tgtaaaacca
    18001 actcgtatgg aattgaataa cttaaaggaa cgtttgaaaa cagctgaacg tggacataag
    18061 ttattaaagg ataaaagaga tgaattgatg aggcgattta tttctttgat tcgtgagaat
    18121 aatcgacttc ggaaagaagt agaaagttat ctaattgata atctaaaatc ttttgcagtt
    18181 gctaaatcat taaagaactc tctaatggtg gaggaattat tttcaattcc atcgaaagaa
    18241 attgaattat ttattgagaa agaaaatatc atgagtgtaa cagttcctag aatgcatatg
    18301 aatattactt ctcaaaatga gaacagtgaa tacagctatt tatcttctaa tagtgaaatg
    18361 gatgatgtat ttgctacaat gaatagttta attgataaat tactaagact ggcagaagtt
    18421 gaaaaaacgt gtcagttaat ggctgatgaa atagaaaaaa cacgtagacg tgtaaatggt
    18481 ttagaatatt cgattattcc aaacttgtcg gaaactattc attatataga attgaaacta
    18541 gaggaggcag aaagagccaa tttagttcgt attatgaaag tgaagtagtt cctttattta
    18601 gatttttaat tagatgaaca aatatcagct tggataaggc ggtaagcctt tctaagcttt
    18661 tttaggctct ttgtcaactg tagtgggttg aa
//
LOCUS       DAVBCG010000028        18225 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBCG010000028 DAVBCG010000000
VERSION     DAVBCG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18225
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.028"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(155..769)
                     /locus_tag="VQ495_000795"
     CDS             complement(155..769)
                     /locus_tag="VQ495_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2530779.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1584..1853)
                     /gene="rpsO"
                     /locus_tag="VQ495_000796"
     CDS             complement(1584..1853)
                     /gene="rpsO"
                     /locus_tag="VQ495_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2530780.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(2079..2624)
                     /locus_tag="VQ495_000797"
     CDS             complement(2079..2624)
                     /locus_tag="VQ495_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2530781.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            2863..3063
                     /locus_tag="VQ495_000798"
     CDS             2863..3063
                     /locus_tag="VQ495_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530782.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            3494..3634
                     /locus_tag="VQ495_000799"
     CDS             3494..3634
                     /locus_tag="VQ495_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2530783.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            3641..3817
                     /locus_tag="VQ495_000800"
                     /pseudo
     CDS             3641..3817
                     /locus_tag="VQ495_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            3875..5818
                     /gene="thrS"
                     /locus_tag="VQ495_000801"
     CDS             3875..5818
                     /gene="thrS"
                     /locus_tag="VQ495_000801"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2530784.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHGDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5867..6841)
                     /locus_tag="VQ495_000802"
     CDS             complement(5867..6841)
                     /locus_tag="VQ495_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2530785.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6834..7511)
                     /locus_tag="VQ495_000803"
     CDS             complement(6834..7511)
                     /locus_tag="VQ495_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2530786.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7667..8740
                     /locus_tag="VQ495_000804"
     CDS             7667..8740
                     /locus_tag="VQ495_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2530787.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHKIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8831..9133)
                     /locus_tag="VQ495_000805"
     CDS             complement(8831..9133)
                     /locus_tag="VQ495_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530788.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9201..9638)
                     /locus_tag="VQ495_000806"
     CDS             complement(9201..9638)
                     /locus_tag="VQ495_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2530789.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9714..10556)
                     /locus_tag="VQ495_000807"
     CDS             complement(9714..10556)
                     /locus_tag="VQ495_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2530790.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10668..11318
                     /locus_tag="VQ495_000808"
     CDS             10668..11318
                     /locus_tag="VQ495_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2530791.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11506..12140
                     /locus_tag="VQ495_000809"
                     /pseudo
     CDS             11506..12140
                     /locus_tag="VQ495_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12271..12708
                     /locus_tag="VQ495_000810"
     CDS             12271..12708
                     /locus_tag="VQ495_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530792.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(12803..13102)
                     /locus_tag="VQ495_000811"
     CDS             complement(12803..13102)
                     /locus_tag="VQ495_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530793.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(13510..13872)
                     /locus_tag="VQ495_000812"
     CDS             complement(13510..13872)
                     /locus_tag="VQ495_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530794.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
     gene            14231..14645
                     /locus_tag="VQ495_000813"
                     /pseudo
     CDS             14231..14645
                     /locus_tag="VQ495_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14684..15127)
                     /locus_tag="VQ495_000814"
     CDS             complement(14684..15127)
                     /locus_tag="VQ495_000814"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2530795.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(15157..17379)
                     /locus_tag="VQ495_000815"
     CDS             complement(15157..17379)
                     /locus_tag="VQ495_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2530796.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17790..18032
                     /locus_tag="VQ495_000816"
     CDS             17790..18032
                     /locus_tag="VQ495_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530797.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
BASE COUNT         5467 a         4045 c         3225 g         5488 t
ORIGIN      
        1 ttttactata tttataagaa tctcctaatc aaatcaaaag ccactctaca atagagtaac
       61 tttaagattg tccatttata ctcaatgaaa aagccctctg aaaatcagag agctgatttg
      121 agctcggact aaaatcctag tgaaacaaaa aaatctacac gatcagaaaa gtctctatcg
      181 tgccattttc atacatgatg tatagtccaa gtagaatgaa tactaagggc acaatgattc
      241 gctcgtattt ttcaattgtc tcgaatatta acggaataga agataacacc cgactaatct
      301 cgcaaaagat aattatgccg attacaaaca caagcaaggc cacgagggtc tgtgaccaat
      361 ctaacgaagc aaaataaggt atatagatac ctaaattatc tccgccagac gcaattgtca
      421 gcaatgtaac tgtccaaaac agttgatttg ccttgctttg ttctaatctt tcaataattt
      481 cttcctcttc ttcttccgca tcttctccaa caattgcaaa gcgaatccct aaatagatag
      541 ggattaaacc aagcaatcca accatccatt cttcaggcac gaaattaacg acataagcag
      601 caactaaact cgcccctaca agtaagcctg tgcctagata ttgccccgca taaatatgcc
      661 atttctgttt attctgtgat agctgtgcaa ataaaataat taaaataatt aaataatcga
      721 tactggtgga aatataaaca ccaatagcag atatgattgt ctgtcccata aaaaaaactc
      781 ctgtattagt cagtaataaa tcagcagatt ttaggagagc acagacacat taatttagct
      841 atcgctagtg ggtaatatcg aacgtaggaa agcattttac tcctcctcaa aacgtagtta
      901 caaagtaatt tctaactgga attcagtgac tacttccatg taaagtataa catactatac
      961 tttactttgt aaagagtggg ctcttctagg gggcttgcat acaactactc ggcttgaaaa
     1021 gccgagtaac tgcgcacctt tatggtgtaa ttagctgata ccgtttgagg tttttgtagt
     1081 ctccaaaatt gttaccgata aaacctcaat aaaaaagccc tctgaagtca gagagctgat
     1141 ttgagtccgg gctaaaatcc tagtgaaaaa gatgaaactc cttgtgctca tcgaacactg
     1201 tgtcatttcc ctattttcat acggattttt gacgccctta gcatcatgat tcttgctaga
     1261 taaatcgttt atagactagg cgacgaggcg aagatcgtac gaaactttca gtgaaacaaa
     1321 aaaaatctcc ccgaagggag aaaagctggt ttattttacc ttacagtgct aggaaccgta
     1381 accaaagatt atacttgagt atatcgaggt aaggtgacaa cacaaaaaag ctacccaatt
     1441 tagagtagct tcattatcaa gatatgctca attgaacacg gcctaagcac ttggcaaaaa
     1501 agataaaatc tcctagaaac tgaagtttct tcgtaaattt tcctattttt actttgtgct
     1561 tttgacagcc tttgtatctt gaattagcga cgaagtccta gagagttgat caactcacgg
     1621 taacggttaa cgtcgttttt acgcaagtat gcaagcaagt tacgacggcg accgattttt
     1681 ttcatcaatc cacggtaagt agcgtggtct tttttgtgtt gtttgatgtg ttcattaagg
     1741 tggttgattt cccaagtaag gacagcaact tgaacctcta ctgaacctgt atcaccttcg
     1801 tgacgtgcat attgtgcgat gatttcattt tttttctctt ttgagattgc catgatgttt
     1861 ctccttttta tttggcttca tccgagtgac aggttggcat gcctgtaacc aagaagaagt
     1921 tatttgtctt tacgacaatc cctattctac caagatttaa attttctgtc aaacattaat
     1981 actctcatag ttattatttt tcaagtaaag agatacgttc ttttatatga tgaccttttt
     2041 caagttcatc taccaatcca atagcataat ctgcataact aatctggctt attccttttt
     2101 tcattagtag taaaaatttc tcctgctaga atgtagtttc ctgttttttc accatcggga
     2161 ataaaatcta cagcaggact gacaaaagcc cagttgagat tattttttgt acgaagaaga
     2221 tccaattcat ccgcttgagc ttttgctagt gacttaaatt cttcaggaaa atcaggcgta
     2281 tctaaaagtc gtgtcgtttt tgtttcatct atataaaggc ttcctgatcc ccctacaact
     2341 agaaaacgtg tctgtgttcc agcaagaagc tgattaaata gttcgattga tttgctgtgg
     2401 agcggtagcg tatctggtgt ataagcacca aacgctgaaa taacagcatc aaatccagta
     2461 agatcatctt ttgtcaactc aaataaatct tttttaataa tagactcagc ttgacttttg
     2521 ttttcagaac gaacaatagc cgttacttca tgtcctcgtt tgactgcttc ttcaacaatt
     2581 gccttccccg cttgtccatt tgctgcaata actgctagtt tcatttttta tacccctctt
     2641 gttgtaatta ttttagttac aacaattgtg acactcttaa taatcaatgt caatagtctt
     2701 gcttaattat tatcaaaata tttctaccaa gaaaactaac catgattcta gtgaaaagaa
     2761 aaatcttctt tgtcaacaaa tttactttct tgttttaaac atactataat aatcatagca
     2821 agagatctaa gttgtctgtt tttttaaaac gaggtgatta tcatgcgtag attctattcc
     2881 catctcccct actatctggt catattattc ttttattggc cactttatga gttgttcttg
     2941 ctagttgttt ctgaccccct tacactcaag ggactctata taaacaatct tctcttcttt
     3001 acacctatgg taatcttgat tgtatcgtta ctctatagct accgtttccg tttctcactt
     3061 tgatggttag ttggtaacgg actgctcttt tactttacta tcataacctt tggtgtgttt
     3121 atactaattt acttgctaat ctatgaaaca gttgctctgg tgggcatgga ttctggtatt
     3181 agcatcaagc atattctaca aaaaatgaaa aacaaaaaac tttcacaaaa tccttgaaaa
     3241 atctcacaat catgctataa taatccatag agacaagtca cttagtccct ttctactaga
     3301 gagtgcgtgg ttgctggaaa cgcataggaa gtctaaactg atactactct tgagtttttt
     3361 atgaaaacat aaaacggtgg ccacgttaga gccgatcaga ggtgtccctc tcttttgagg
     3421 tacataaatg aaggtggaac cacgttgcga cgtcctttcg aggatgtcgc atttttttat
     3481 taggatacta attatggagt tgcaagaatt agtggagcgc agttgggcaa tccgacaagc
     3541 ttatcacgaa ctggaagtta agcatcatga tttcaagtgg acggtagaag aagacctctt
     3601 ggctttatct aatgatattg gaaatttcca atgactggtg atgacaaagc aaggacgcta
     3661 ctatgatgaa acaccctaca cactggaaca aaaactttca gaaaatatct ggtgactatt
     3721 agaactttct caacgtttgg atatagatat tctgacggaa atggaaaact tcctctctga
     3781 taaagaaaag caattgaacg ttagaacttg gaagtagtct gctgataaaa aatcaatgct
     3841 tagaaactat gaaataataa aaaaggagaa catcatgatt aacattactt tcccagatgg
     3901 cgctattcgt gaattcgaat ctggcgtaac aacttttgaa attgcccaat ctatcagcaa
     3961 ttccctagct aaaaaagcct tggctggtaa attcaacggc aaactcatcg acactactcg
     4021 cgctatcact gaagatggaa gcatcgaaat tgtgacacct gatcacggag atgcccttcc
     4081 aatcttgcgt cactcagcag ctcacttgtt cgcccaagca gctcgtcgtc ttttcccaga
     4141 cattcacttg ggagttggtc cagccatcga agatggtttc tactacgata ctgacaacac
     4201 agctggtcaa atctctaacg aagaccttcc tcgtatcgaa gaagaaatgc aaaaaatcgt
     4261 caaagaaaac ttcccatcta ttcgtgaaga agtgactaaa gacgaggcac gtgaaatctt
     4321 caaaaatgac ccttacaagt tggaattgat tgaagaacac tcagaagacg aaggcggttt
     4381 gactatctat cgtcagggtg aatatgtaga cctctgccgt ggacctcacg ttccatcaac
     4441 aggtcgtatc caaatcttcc accttctcca tgtagctggt gcgtactggc gtggaaacag
     4501 cgacaacgct atgatgcaac gtatctacgg tacagcttgg tttgacaaga aagacttgaa
     4561 aaactacctt caaatgcgtg aagaagctaa ggaacgtgac caccgtaaac ttggtaaaga
     4621 gcttgacctc tttatgattt cacaagaagt gggacaaggt ttgccattct ggttgccaaa
     4681 tggtgcgact atccgtcgtg aattggaacg ctacatcgta aacaaagagt tggcttctgg
     4741 ctaccaacac gtctacactc caccacttgc ttctgttgag ctttacaaga cttctggtca
     4801 ctgggatcat taccaagaag acatgttccc aaccatggac atgggtgacg gggaagaatt
     4861 tgtccttcgt ccaatgaact gtccgcacca catccaagtt ttcaaacacc atgttcactc
     4921 ttaccgtgaa ttgccaatcc gtatcgctga aatcggtatg atgcaccgtt acgaaaaatc
     4981 tggtgccctc actggccttc aacgtgtacg tgaaatgtca ctcaacgacg gtcacctatt
     5041 cgttactcca gaacaaatcc aagaagaatt ccaacgtgcc cttcagttga ttatcgatgt
     5101 ttatgaagac ttcaacttga ctgactaccg cttccgcctc tctcttcgtg accctcaaga
     5161 tactcataag tactttgata acgatgagat gtgggaaaat gcccaaacca tgcttcgtgc
     5221 agctcttgat gaaatgggcg tggactactt tgaagccgaa ggtgaagcag ccttctacgg
     5281 accaaaattg gatatccaga ttaaaactgc ccttggaaaa gaagaaaccc tttctactat
     5341 ccaacttgat ttcttgttgc cagaacgctt cgacctcaaa tacatcggag ctgatggcga
     5401 agatcaccgt ccagtcatga tccaccgtgg ggttatctca actatggaac gcttcacagc
     5461 tatcttgatt gagaactaca agggggcctt cccaacatgg ctggcaccac accaagtaac
     5521 cctcatccca gtatctaacg aaaaacacgt ggactacgct tgggaagtag ccaagaaact
     5581 ccgtgaccgc ggtgtccgtg cagacgtaga tgagcgcaat gaaaaaatgc agttcaagat
     5641 ccgtgcttca caaaccagca agattcctta ccaattaatt gttggagaca aagaaatgga
     5701 agacgaaaca gtcaacgttc gtcgctacgg ccaaaaagaa acacaaactg tctcagttga
     5761 taattttgtt caagctatcc tagctgatat cgccaacaaa tcacgcgttg agaaataaga
     5821 gtctagcata aaagcctcca atctggaggc tttttctcat ctatttttac tcaaggacta
     5881 agttcacttg agcaaactga atccgcactg tcgttccttt tccgacctca gactcgatac
     5941 gaatctggtg ccccagttct tcagaaattt tcttagatag ataaaggcca agtccagagg
     6001 actgctgggt caaacggcca ttgtatcctg aaaagccacg ttcaaatact cggaggacat
     6061 cactgttttt tatcccgatt cccgtatctt tgatacaaag ctcttggtca tccatataaa
     6121 tctccagacc accttccttg gtgtacttga gactgtttga gatgatttgc tcaataacca
     6181 ctagcagcca ctttttatcc gtcacgattt ctttatcaag gtcatgtaga ttgacattta
     6241 agcctttttg aataaagaaa agagcatatt tacgaattat ttccttgacc aagtcctcaa
     6301 tttgaacctg ctttaagagc aaatcatcat ggaaactttc taaacgcagg tactgtaaaa
     6361 ctaggttggt ataagagtcg attttgaaaa tttcctgttc tagctgctgc ttcagttggc
     6421 ggtcgaccac ttctgcaact aagagttgac tggctgcaat gggggtcttt atctgatgga
     6481 cccacaaggt atagtaatcc agcaaatccg tcagttttct ttctgctttt gacctctgct
     6541 gatagagttc catctcacgc gcttctaatt tttctgctaa agctatttcc aaaggagact
     6601 tggcttccct ctctccatag agaagttcct ggcgatagac ctgcatttcc accaatatgt
     6661 cccaagtgaa aaataagatg gttacaaagc aacacaagaa gaaaaagtag aggaagtaaa
     6721 ttcctagact ggcaaataaa aactgaaaga gtaagacaag aaatgccaaa gaaagcagat
     6781 agataaaaag acgactacgg gagcgcagat aggctagaaa aaattgtttc caatcaagca
     6841 tgcttcaatc cgtaccctat tcctttcttg gtctcgataa atcctaccaa tccctgctcc
     6901 tccaactttt tacgcaaacg agccacattg acagagaggg tattatcatc aatgaaaaag
     6961 tcactgttcc aaagttcccg catcaggtcg tcacgtgcta cgatgttgcc tgcatgctca
     7021 aataacacgc gtaaaatctg gaattcattc ttggtcaaat tcaagacttg cccttgataa
     7081 tgtaaatcca tggatttggt attgaggata acaccagcat attccagcaa actctcatca
     7141 cgcccaaact cataggaacg acgcaacaag ccctgaacct tagctaaaag aacctgctgg
     7201 tcaaaaggct tggtcacaaa gtcatccgcc cccatattga ttgccatgac aatatccata
     7261 gcctggtctc tcgaagaaag aaacatgata ggtaccttgg aaatcttgcg gatttcctga
     7321 caccagtgat aaccattaaa caagggcaaa ccaatatcca tgaggaccag atgaggttcc
     7381 gactgaacaa atagactcaa aacttccata aagtcttcta ccaggaccac ttcaaatccc
     7441 cattcagaga gcattttccc aatctgttga cgaatgacct gatcatcttc tattaataaa
     7501 atcttgtgca tgcgcttctc cttttccatt attataacag atttttccat gctagatggt
     7561 ctgaaactga atttgaaata gcctgttttt agccagtgca aacaggctat gctactagct
     7621 aatttgaggg aaatttgcta agataaataa aaagaaagga gctcttatgg ccaatatttt
     7681 tgactatctg aaagatgtcg catatgattc ttattacgac cttcccttga atgagttaga
     7741 cattctaacc ttaatagaaa tcacctacct ctcctttgat aatctggtct ccacacttcc
     7801 tcaacgtctt ttagatctag cacctcaggt tccaagagag cccaccatgc ttactagcaa
     7861 aaatcgcctt caattattag ataaattggc tcaacacaag cgcttcaaaa attgcaaact
     7921 ctcccatttt atcaacgaca tcgaccctga actgcaaaag caatttgcgg ctatgactta
     7981 tcgtgtcagc ctcgatacct atctgattgt ctttcgtggg acagatgaca gtatcattgg
     8041 ctggaaggaa gatttccacc tgacctatat gaaggaaatt cctgctcaaa agcacgccct
     8101 tcgctattta aagaactttt ttgcccatca tcctaagcaa aaggttattc tagctgggca
     8161 ttccaaggga ggaaatctcg ctatctatgc tgctagccaa attgagcaaa gtttgcaaaa
     8221 tcagatcaca gcagtttata catttgatgc acctggtctc catcaagaat tgacacagac
     8281 tgcgggttat caaaggataa tggatagaag caagatattc attccacaag gttccattat
     8341 cggtatgatg ctggaaattc ctgctcacaa aatcatcgtt cagagtactg ccctgggtgg
     8401 catcgcccag cacgatacct ttagttggca gattgaggac aagcacttcg tccaactgga
     8461 taagaccaac agtgatagcc agcaagtaga cacaaccttt aaagaatggg tggccacagt
     8521 ccctgacgaa gaacttcagc tctacttcga cctcttcttt ggcactattc ttgaggctgg
     8581 tattagctct atcaatgact tggcttcctt aaaggcgctt gaatacattc atcatctctt
     8641 tgtccaagct caatccctca ctccagaaga aagagaaacc ttgggtcgcc ttacccagtt
     8701 attgattgat actcgttacc aggcatggaa aaatagataa tactcttgaa aattaaatgt
     8761 atacaaaaca aaagacctag aatacatact ttcatgtgca ttctaagtct ttttaaatag
     8821 aatctaatag tcaataaaaa tcaaagagca ttgagagata atggggcttg gaacgtccct
     8881 ctcgcttcaa caaaatgacc ccattataga ttaaaaagat gccacttaga aaaagcaaaa
     8941 aaggaagtaa gacaaaggca aatacataaa aagctaactg aacattctcg tatccatttt
     9001 tataaaaaag gtaggataga taaaaataac ttgaaatgag ggataataaa aataatactg
     9061 gattccacaa acttctatta tccttccaaa atgacactat aaaggctaat acaattccta
     9121 taacgagata catttcttac tcctttaata gctacatttt atcataatta tccaaagaaa
     9181 aaagagggca tttatccctc ttaatccttc atctgactct ctgcatcggc cacgactttt
     9241 tctagactgg tttgaccaag ttctgcctcc atagtcaact gaattctctc caatttttga
     9301 tccaaaacat catgaatatg agctcctaca gggcaatttg gattcggatt gtcatggaaa
     9361 ctgaagagtt gacctgtctt accaagacat tcgaccgcct gataaacatc taaaagacta
     9421 atatccttaa ggtccttgac aatatctgtt ccgcccgttc cacgcgctac tgaaatcagc
     9481 tctgccttct tcaactggga caagatcttt ctgataatga caggattgac cccgacacta
     9541 gcagccagaa aatcactggt caccttgctt ttcttcccct cgagggcaat gattatcagc
     9601 atatgagtcg caatggtaaa tctacttgga atttgcatcc tcttctcctt tttacgaggc
     9661 taccctgcct ctactcttct ttttctatta ttataccctt tttagttgta atgtcaatcg
     9721 ttaccacttt tcaaccagtc gtctaactcc cgatcgcaac cctctttctg agccaattct
     9781 ctcaaaaatt cctgatgatg agtatggtgg atcccattga ccagactttc atagtaaacc
     9841 tcaaaatagg gaagtctcag gtctttagcc agctgcaatt cagctgccac atcgtaatct
     9901 acccgtcgga agtccatatc taccaggccc ttgtcatcaa actccaaaat catatactgg
     9961 gcccgcaagt ccttccgtag ctgagcgtcc aaaaagaaag gttggccaat cgaacccgga
    10021 ttgacaatca attgcccacc agtcccgtaa cgaagcaact gctggtgaat gtgtccataa
    10081 acagcaatat cacagggagg atgagtcacc aagcggtcaa attcctcttg tttgccagta
    10141 tgaatcaact ctcgccccca gttcttatca ggcagatgat ggctaattcc cactgtcaaa
    10201 tccccaaact gacgatgaat ttgaagaggt tgattgtgga gcacttcaat ttcttctagg
    10261 gaaatttcct ctaaaacata ctggcactgg cgcaagagat agcgttgact agggcgagta
    10321 ctatccaatt ccttacggac accatgccaa agactgtctt cccagtttcc caaaactcta
    10381 gccgtaatcg gtagttgatc caacaagtcc aaaatccttc tacgccctgt ccctggcatg
    10441 agaatatctc ccaaaagcca gtattcatcc actcctagct gccgagcatc tgccaaaaca
    10501 gcctccaagg cggtggtatt tccatgaata tctgaaagaa gagctatttt cgtcatatcc
    10561 atctcctcgt tttttctctt gcaataagta taacataaaa agtcacagct agagaaatct
    10621 agcttttttt gatatactag ataaagatat tagacaagag gaaacgaatg accccaaaca
    10681 aagaagacta tctaaaatgt atttatgaaa ttggcataga cctgcataag attaccaaca
    10741 aggaaattgc ggctcgcatg caagtctctc cccctgccgt aactgaaatg atcaaacgaa
    10801 tgaaaagtga aaatctcatc ctaaaggaca aggaatgtgg ctatctactg actgacctcg
    10861 gtctcaaact ggtctctgag ctctatcgta agcaccgctt gattgaagtt tttctagttc
    10921 atcatttaga ctatacaagt gaccagattc acgaggaagc tgaggtcttg gaacacactg
    10981 tctctgacct gttcgtggaa agactagata aactgctagg tttccctaaa acctgccccc
    11041 acggaggaac tattcctgcc aagggagaac tcctcgttga aatcaataac ctcccactag
    11101 ctgatatcaa ggaagctggc gcctaccgcc tgactcggat gcacgatagt tttgacattc
    11161 tccattatct ggacaagcac tcacttcaca tcggtgacca gctccaagtt aagcagtttg
    11221 atggcttcag caataccttc actatcctca gtaacgacga ggatttacaa gtgaatatgg
    11281 acattgcaaa acaactctat gtcgagaaaa tcaactaatt tctcaagtcc cctaccaacc
    11341 ctgaaagttt tattttcagg gttttatttc tatcatttta ttttactttc atttggtggt
    11401 agaatatttg aaaaaaagaa aggtagttta acctatgaaa aaatcactaa aaatttttgc
    11461 tacatctaaa tagtttgacc tcttcggggt tgctttggtc gttggattgc gattgcatct
    11521 ggttacctca actcacgcct cgataaattt gtagactggg gaccatggac agctcttgta
    11581 ccctttggat tgatttccgt aaccaacgtt gggatttcca tgttgtccac tcgtttcacg
    11641 gggaaattaa gcaaatggga aaattacttt ggtattgtta ataccatttt gtccggtgct
    11701 attgattata tccttggaaa taaggcggcc atcattacct atcccgtcac cttcctcatt
    11761 tatacctttg ctataaagaa atggaaagct tcgcaagaag gcagacccaa ccaaatgagc
    11821 caaaaacagg taaaattggc ggccatcatc atttccatca tcgccttcct ctttgccttt
    11881 gtgaccaact atatcggata tgggggcaag atgaatctcc ttgcctacgt aacaactatt
    11941 gcctttgcac tgtccctcat tgccaatgct ttgaatgcat tggcaatgag ggacagtggg
    12001 gcttttggtt gatttacaat ttcgttcagc tgacaaaagc tggtattcaa ggaaacttcg
    12061 ccaatatcgg taaatacatc ttttatatcc tcaatgcaat cggagcttta tttgtctgga
    12121 atgatgaaga agttagataa tagaaggaaa ctcaaataaa gaatactagc caacttaaga
    12181 gaatctcccc tctgctattt ttatcatttt agttgtaact cccattcttt tagagtagaa
    12241 tgaagttaga taaaagaggg aggtcttgtc atgaaaaaac tctatagaat ccattttata
    12301 gcaattgcag ttattgattt gctactattt gcatttttta tcactagact cgagacatct
    12361 tttgagtggc tcttgctctc tggtttaatt ttcttccttg ctcaaggact cctgctattt
    12421 ctcttggtcg tccgactcaa acatcaattc gctgagattt atcctcaaat caataaaaag
    12481 attcgcttct actatttagg ggttctcacc attgattttc tattttttgt tctcttagcc
    12541 ttcattagtt ctcagcgttt ttcatctctt atgccaatca tcactgcttg ccattctact
    12601 ttttattata tgacagctga ctacctaaga gaaaactatc cagactttta cgacaaacac
    12661 atctctaaca cgtatactat tttatcgatt tcttcttttt tagtataaga cagctcgttc
    12721 tccataaata ttcattaact ttcattttta tacttttcct ttcgattttc aaaaacaagg
    12781 ggagggatta acctccctta tattagacat aacttttagg atcacaattg tatgttcctt
    12841 tcgaaatatc cccaaaaatc gtagcccctg ttaaataatg atttcttgta gaagcatatg
    12901 ctattttctt catcacatcc agtctcgcta cttttagctc gagattagga ttggaaaaca
    12961 tagcattttt aatatcttcc aaatcttgag aactaggtgc gagtagagtt acatcgccct
    13021 ccaatctatt agccttgtct atataaagac taactctttc agcactttct gttcgcagaa
    13081 aatttagtac atttctattc atttttttac ctcgtatttt tctaattatt gattttttct
    13141 agaaacaaaa tcaattcaac ggtgaagctc tagctttcat cttcattcgt acctcctttt
    13201 gtgtatatct agctttacat aaacctatga tttagagtat agtttctcaa ttattctcag
    13261 aaactatgga gatgatttaa ttatcataca aatcaaagac ctctccttgc tcatcttcac
    13321 cgtaactctc gagttcctca taaaagtatt ccaaagcagt ttgttcttct ggtgtcataa
    13381 agaaactcct ttctaaacaa tattagtaaa aaatacgttc ttatttccat cctaaataaa
    13441 gtagtgtttt tatcccgtaa taggttttat ttttgcggag cattacactc cgcattaata
    13501 ttgatagtat taagaatcat tattacttaa taaaaattgc gctctcaaac atagacgtat
    13561 acaaaatgcc ctatgtatac gtttcgcttg gaagacatct aagaaaaaat tttgtagttc
    13621 agacagtaag attaatacat ctttctctaa cataagtgat gtctggtact cccaattatt
    13681 gctctgtttt aacgatgaaa aaggtatagc tgtctctttt aattttatcc aatctatacg
    13741 ttctttttta ggaacgtatt ctctaacagc ttgactaaca atactggaat agcttacttc
    13801 atagcctaat ttattgtaaa catcatcttt aagcttatct aatatctgtc tggattcaga
    13861 cgctaaacgc attctaacat tctcttttcc catattatca cccctttctg acgggattct
    13921 gttatcccgt aaattaagta tataacaatt tagaaaaaaa atcaagaatt aagttgaaaa
    13981 aaattccaca atctaaaaat ttatcatttt atgacaaaca tactatcgtt aaatgctgat
    14041 ttaatcgaaa ttttgagtta taaactcctc agtttcgaac tcaaaattag ccgtgcagga
    14101 taggataccc ttcataaact tatataacta ctttaatacc ttaaaaagct aataaaataa
    14161 ggaatatcta agcaatatac attctaaaat acctgtacgg gagtgtctct aaagaaaagg
    14221 aggttttagc atgaaaaaaa tcatcttcat caaaaccatt caactccttg tcattgatgg
    14281 aatcatgctg gcatttttga catttaaaag gggggcttac ttgggactgg attttgattt
    14341 atagtggttg gctcattttc tttcatcctg tgctattgac ctatctttca aaccaacttt
    14401 gtgaccactt tagtcaactc tattcccaga ttagaccgag attctggcgt tttgctttac
    14461 aaattctcct atgggatagc ctgatgattc tctccttggt gtctttaagt gatattccac
    14521 ttttccttca gggaactctc ctcatcctag gacatctcat cccttcctat cgcatctgcc
    14581 aaagcctgaa aagagacttc ccccaagcat atcaagaacc gatttctttt tggagtattt
    14641 tatgatcgat gagaaagacc aagccgactg ggcttggtct ttcttatctc tttttagtat
    14701 ctaggataat ggtaacaggt ccgttattaa ccagctcaac ctgcatatct gctccaaaga
    14761 tacctgtctg aacgggcact tcttgcgcta atttttgatt gaaagcatca tagaagtctg
    14821 atgccatatc aggtttagct gcccctgtaa aggctggacg attgcctttc ttagtatccg
    14881 caaagagggt aaactgagaa atagagagga tttctccttc aatatctttg acagacaggt
    14941 tcatcttgcc ttctgcgtct gaaaaaatcc gcatattgac cagttttctc acagcatagt
    15001 ccaaatcttc ctcttggtcc tctggtccaa caccaaccag caataaaagt ccctgattga
    15061 tttttccctg aatctggcct tctatactca cttgggcttt tttaacccgt tggataatga
    15121 ttttcataat agcctttcta gtaagagcta ggacaactag ccgttggtcc gtttgacaga
    15181 gtaaacttct ggcacactct taattttatc gacaaccgtg gtcagtgtag agaggttggc
    15241 aataccgaag gacacatgga tattagcaaa cttcatatcc ttggttggtt gggcattgac
    15301 cgttgaaata ttcttggttg tatttgaaag aacttgcagt acatcgttca acagtcctgt
    15361 acggttgaga ccgtagatat cgatatgggc catatactcc ttatttgagc tagagtactg
    15421 gtcttcccat tccacatcaa ggagacgttg ctcgtagttt tcttgggcac gcaggttcat
    15481 acagtccaca cggtgaatag ccacaccacg acccttggta atgtagccaa caatatcgtc
    15541 accaggcacg gggttacaac acttagcaat ccgcactagg agaccagaag caccttcaat
    15601 aaccactccc ccctcatgct tgaccttgag agtttcttta ttttcaacct tgacctcgcc
    15661 acctttgaca agctcctctg cctcagcctt ggccttggca cgctcttcct cacggcgttc
    15721 cttttcagtc agacggttaa agacggtaat cgcaccgatt tccccaaaac caatggccgc
    15781 aaagagggag tcttctgtct tgtaactggt cttttgcaga acttgatcca tgtggcgctt
    15841 gtccataaat ttatttgcca catagccatt ttcttggaac tgagccatca gcatctcacg
    15901 acccttgttg acagacaatt ccttatcttg gtttttaaag aactggcgaa tcttattgcg
    15961 cgccttgcta gtcttgacca tattgagcca gtcacggcta ggtccaaagg agttcgggtt
    16021 ggcgataatt tcaacctgat cccctgtctt taacttggtt gtcagtggaa ccatgcggcc
    16081 attgaccttg gcaccagttg ctttttcacc gaccttggta tggatttcgt aggcaaaatc
    16141 aatcggtcct gaatctttgg gaagagaacg gacagctcca tctggggtaa aaacgtaaat
    16201 ctcctcagcc agatagtttt ccttaacaga gtccacaaat tccttagcat catcagcctg
    16261 gtcttggagc tccatcatct ccttgatcca gttcattcca atagctgatt ccttgctgtt
    16321 aacttgcccc tttatacctt tcttataagc ccagtgagcc gcaaccccgt actcagccac
    16381 ctcgtgcatt tccttggttc gaatctggaa ttcaatcggc ccttttggtc cataaacagt
    16441 cgtatggata gactgataac cattagcctt gcggttggca atatagtctt tgaagcgacc
    16501 tggcatcggt ttccaaaatt catgcacgta accaagcatg gcataaacat cactttgggt
    16561 atctaaaata caacgaatag caatcagatc atagatttcc tcaaaccgtt ttctcttgtc
    16621 ctgcattttg cggaaaattg agtaaatatg cttgggacga ccataaatct tccctttcaa
    16681 gtgacgttct gtcgtatact cctctaattt tgtgactacc tcatccacca aggcctcacg
    16741 ctccctgcgc ttttccttca tcatatgggt aatcttgtaa aactccgttg gattgagata
    16801 acggaaagac aagtcttcta attcccattt gacactggaa atccccaaac gatgggcaag
    16861 cggggcatag atttccatgg tttctttgga aatacgctcc tgcttgtctt ttcgaagatg
    16921 tttcagggtc cgcatattgt gcaagcggtc agacagtttg accaaaataa cgcggatgtc
    16981 ctcagacatg gccatgagca tcttgcgatg attttccgct aattgctcct cgatcgattt
    17041 gtactcgacc ttgccaagct tggtaactcc gtcaacaatc atccgcacat caggaccaaa
    17101 ctctctttcc aaatcgtcca aagtcgcatc tgtatcttcc accacatcat gcaagaatcc
    17161 acaagctact gttacagcat ccagctttag cttagctaaa atacctgcca cttggatagg
    17221 gtgaatgata taaggctcgc ctgatttgcg atattgacca ctgtggcatt caacagcata
    17281 gaccaaggcc ttatggacaa aatgaacatc ctcttccgtt aaatattctt tggttaaagc
    17341 gacaacttct tcgcctgtta aattcacttc tttcggcatc tctactctcc aattcttcct
    17401 accattttat cactttttta agaatatgaa aactagattg gaacagaata agaaaaaaat
    17461 aattcaaaat tgcttgataa ttctgaatta ttggcccata atatactacg aagttagatt
    17521 ttaaacttag gtgatagaag gagagataga agaacggaaa ccatattgta acccaaagac
    17581 tttctgattt ccccaattcc attgaagata cgaaagataa acggtggaac tcgtatcaca
    17641 tacactggta ccttgactgg attttggaat taatactcaa tgaaaatcaa agagcaaact
    17701 aggaaactag ccacaggttg ctcaaagcac tgctttgagg ttgtagataa gactgacaaa
    17761 gtcagtcaca tatatacggc aaggcgacgt tgacgcggtt tgaagagatt tttgaagagt
    17821 ataaaaatcc tcaagatact ttcttctatc ctttagttta taaggagaat acctatgaaa
    17881 aaactgctat ttctatcttt gctctcctaa tgttaggagt ttgctgcctg ttcctattca
    17941 gccagcaaag ctataaaaaa ctcgttcaat actatgctaa cgaccagaac ctgcccagta
    18001 ggataactta tagtgaatat agcgacaaat gagaagccaa ctacggtagc actctaaaca
    18061 tcacgtctat caaacaagct aatgacggag tttatgcaac ctatgaaggg caattgacac
    18121 ctttccaata ttgataaatt gataaccagc ctgtcttcat ctagtcatgc tggtttttaa
    18181 gttcatttta aatccttacc tattctccct aactgtgcta tactt
//
LOCUS       DAVBCG010000029        18115 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBCG010000029 DAVBCG010000000
VERSION     DAVBCG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.029"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..150)
                     /locus_tag="VQ495_000817"
     CDS             complement(<1..150)
                     /locus_tag="VQ495_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530798.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGIL"
     gene            complement(159..1094)
                     /locus_tag="VQ495_000818"
     CDS             complement(159..1094)
                     /locus_tag="VQ495_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2530799.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(1091..1816)
                     /locus_tag="VQ495_000819"
     CDS             complement(1091..1816)
                     /locus_tag="VQ495_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2530800.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(1900..2535)
                     /locus_tag="VQ495_000820"
     CDS             complement(1900..2535)
                     /locus_tag="VQ495_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2530801.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(2537..3364)
                     /locus_tag="VQ495_000821"
     CDS             complement(2537..3364)
                     /locus_tag="VQ495_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2530802.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(3386..3722)
                     /locus_tag="VQ495_000822"
                     /pseudo
     CDS             complement(3386..3722)
                     /locus_tag="VQ495_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(3723..4439)
                     /locus_tag="VQ495_000823"
     CDS             complement(3723..4439)
                     /locus_tag="VQ495_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2530803.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSKTVEKDGDKKLIIY"
     gene            4962..5573
                     /locus_tag="VQ495_000824"
     CDS             4962..5573
                     /locus_tag="VQ495_000824"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2530804.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            5618..6346
                     /gene="rlmB"
                     /locus_tag="VQ495_000825"
     CDS             5618..6346
                     /gene="rlmB"
                     /locus_tag="VQ495_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2530805.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(6385..7296)
                     /gene="trxB"
                     /locus_tag="VQ495_000826"
     CDS             complement(6385..7296)
                     /gene="trxB"
                     /locus_tag="VQ495_000826"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2530806.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(7362..7586)
                     /locus_tag="VQ495_000827"
     CDS             complement(7362..7586)
                     /locus_tag="VQ495_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2530807.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(7664..8407)
                     /locus_tag="VQ495_000828"
     CDS             complement(7664..8407)
                     /locus_tag="VQ495_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2530808.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILLLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(8407..9207)
                     /locus_tag="VQ495_000829"
     CDS             complement(8407..9207)
                     /locus_tag="VQ495_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2530809.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(9365..9721)
                     /locus_tag="VQ495_000830"
     CDS             complement(9365..9721)
                     /locus_tag="VQ495_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2530810.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(9734..10246)
                     /locus_tag="VQ495_000831"
     CDS             complement(9734..10246)
                     /locus_tag="VQ495_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2530811.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(10246..10740)
                     /locus_tag="VQ495_000832"
     CDS             complement(10246..10740)
                     /locus_tag="VQ495_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530812.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            10875..11321
                     /locus_tag="VQ495_000833"
     CDS             10875..11321
                     /locus_tag="VQ495_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2530813.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            11318..11965
                     /locus_tag="VQ495_000834"
     CDS             11318..11965
                     /locus_tag="VQ495_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2530814.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(11986..12567)
                     /gene="pdxT"
                     /locus_tag="VQ495_000835"
     CDS             complement(11986..12567)
                     /gene="pdxT"
                     /locus_tag="VQ495_000835"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2530815.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(12568..13443)
                     /gene="pdxS"
                     /locus_tag="VQ495_000836"
     CDS             complement(12568..13443)
                     /gene="pdxS"
                     /locus_tag="VQ495_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2530816.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(13595..14974)
                     /locus_tag="VQ495_000837"
     CDS             complement(13595..14974)
                     /locus_tag="VQ495_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2530817.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            15269..16192
                     /locus_tag="VQ495_000838"
     CDS             15269..16192
                     /locus_tag="VQ495_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2530818.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            16252..16857
                     /locus_tag="VQ495_000839"
     CDS             16252..16857
                     /locus_tag="VQ495_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2530819.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            16875..>18115
                     /locus_tag="VQ495_000840"
     CDS             16875..>18115
                     /locus_tag="VQ495_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2530820.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         5338 a         4143 c         3241 g         5393 t
ORIGIN      
        1 gagaattcca ccagcgatta aaagacgcaa gttggtcaag accagagccg aaattccgtg
       61 tgccattagg tattggccac tcgttcctga caacccccaa gcaataccag ccacaactgt
      121 tagtaaagtc ccttttaaac tatttgacat gtctctcctt actcttcttt gcgaatcaag
      181 ttcatcactt gattgcggtc taatatccat tgagctctct cgtccttttc ataggtacga
      241 ttcgatagga aaatagtggc ttcttgcttc tgacgattcc acatgataaa ggtacctgta
      301 tagcccgtat ggtctagcca atctccttcc aaattccatg ctaaagaacg ttccttgtca
      361 tccaaaggag aaaaattttg atttaagtct cttgcaaaat catctgctaa atagtgttct
      421 aaaaagattt gtaaatcctt tatagtcgaa aataaaccag cactcccagc atgtctaccc
      481 aggagacgag ccttgggatc atgcactatg cctgcctcta cacctctaac tgttggaaca
      541 gcaagctcaa ctggcccaaa cttggtttcc gtcattcccc aaggtttcca gacttgatcc
      601 tttaaaatca catccaaatc ttgattaaaa attctttcca aaataaagcc caacagcaaa
      661 aaatggacat ccgaataaag aaaggctggc tgacttcgtc tgttgagatg aaacatcgct
      721 tcctttaatt caggggctgt taaaagatca cgattaggaa taaaaggatc aaggtctgtt
      781 gcatgagtca agagctggcg aatagtgatg tctggataat cactctcagg taaaaaatct
      841 attaccagtc tatcaatatc taattgacct atttcccaca agaaggtaca aactgtgcca
      901 accccaacaa ccttgctgac actagctaga tcataaacta gtcctgcctc agtctgcaag
      961 ccatgctctg ggtcactctg gcctaaatag aactctgtcc attgattgtc cttaaaatac
     1021 gcaaaagagg ctccgggata aattcctgcc tcgatttgtt cttctatttt tttaataatc
     1081 ttggtccact tcatacttct tcaaaccaca attcaacatt atttctatct ttaccaagga
     1141 agaatttttc agacttagga ataaaatatt cagtagactc aaacttctgg cgaagacttg
     1201 ctatgtctaa ttcattgacc aagaacttga gcatagataa gtcccaggta accgtattgt
     1261 ccacagtcaa atcctgcccc tgagctggaa taaaatcaag ggatgcccca atttcagatg
     1321 attccaagaa actttcgata tcagttggga gatgtaattc catagaaatc tcaaatttac
     1381 ttaaagaaat tgatgccaaa tctgtttgaa attcaggctt ttctcctact tctactagac
     1441 ttgctctgtc atcttccgca tgaatcaaaa tcaaatcatc ttctggtgag aaaatttcaa
     1501 aagcgtagcc attttgacct ttatataatc gatgaatcga atctgtttta gataagattc
     1561 cttcaatttc taagggattt tccaccttga caatcaatct agctagtttt tttcttccct
     1621 ctaccttacg agtacgcata ctgggagctt cttctaaaac cagcttttca agacccgttt
     1681 ggtcacctag tgacagaaag gccgattctt ctaacaaggc cttcattcca agggtttcaa
     1741 tataaaatgt ttcatttaat tttctattat tagcttttaa agtaggaata atccgtacaa
     1801 tctgatttac attcataaat tcctccgtca ctttttattt taaaggattt tagaattttt
     1861 tacaagatat tatgctcaaa tttaacatta tttagctatt tagtaaagat agtctttcaa
     1921 ggattttgac aaagcctgat aaccagcaat actgaggtgc agtccatcag ttgtatattc
     1981 ttttttgagt tggcctgctt ggtctgtcaa acaatcaaat actggcacaa attccacctg
     2041 catataggca gatgcaagct cttgataggc ttgattccag ttctgaattt tttcattcga
     2101 gcggatatag actgcctgct ggtactcctc tccctcattg acaggcaaaa tggaaagcaa
     2161 tttaatctct gtcaatggat aatcgcgagc aacggattga atgatagctt cgagattatt
     2221 gagagcctca ttcacaggaa catcctttcc gatatcattt gtcccaatca gaagaaaaat
     2281 tttatctact gctccaccat atagatgagc atcaagattc tctagtaaca gtcctgtctg
     2341 atagccacga attcctcgat tgacaatcgt ctttgaagtc ccaaatagct cctgtagagg
     2401 ataatactcg acaatggaat ccccaataaa aagaatgttt ggttctacaa cagaaatgtg
     2461 atttaggtga cgatacttag tttgaatttt ttcttgttcc tttaggagcc aattttctaa
     2521 taactgtact gccacttcat tctccttttt ctaaccagtt ttccaagact tgataaactc
     2581 ctccttggct gttggctggt gctagagcag tcgccacagc tttggctttc tcatcagcat
     2641 tttccatcgc ataggcaatt ccagccattt caagcatttc aacatcattt tcactatcac
     2701 caaaagccat gatttcttgg gatttcaagt cccagcgctt gagtaattcc tccaagcccc
     2761 atgctttatg aatcccagct tggaggatat caatgcaacc atagccactg gatacagctc
     2821 ggacacggcc atcaaagaga gcattgattt cttccaaaac cgaactcaaa cgttcctcac
     2881 caacaaccat gctcatcttg agcacaccac caaagaggtc agatgttaat tcatccacaa
     2941 attgcatccg ttggtagaat ttttcaatca tttctggagt cataaaactt tcaagatctg
     3001 taaaaatcgt accttccttg acaaaatcac ccttcatccc cgttacaaca aactggtcct
     3061 gacacgctcg acccttgaaa tgagccaaag ccttgttgac aatggcgtca tcccatgtct
     3121 gagcctgaat caattcattg ttttcaaaaa tacgagcgcc attagcaaca accagaacca
     3181 ctcgatccac caagggactc agtagttgtc tcatgcggtg aatttcattg cccgtcgcaa
     3241 tgacaaaacg aatgcccctt tgatccaact gatctaaaat cttttccaat cgtgggagat
     3301 caagctgacc cctagcatcc agcaaggtcc catccatatc tgtcgcaatt accttaatca
     3361 tcatttactt tcctctggat tcaagctaat accacttcaa tcccacatgt tcattcattt
     3421 ctttataggc tgtatcaatt cgttccttag tcgcttcatc taactggtca cgatgactgc
     3481 cacccctcta taaaatcttc caaaatataa gtttggtaat gatacaaggg atatccttct
     3541 ggtgaaaagg ttccctttgg tgttcgattg acccaagtag gatgagcttt agctgttccg
     3601 atagttgttt ttccatcctt cttcttgatg gtgacatcca tgagaacacc acgttcagtc
     3661 cacttagcat tctcttcatc tcccatagat tcaatccgtt gattggaaat gaagttcccc
     3721 atttaataga taatgagttt cttatctcca tctttttcaa ccgttttaga tggttcaaca
     3781 acgtgaggat gccctccaaa gataatatcc gctccccaat cgatcatctt gtgataaaga
     3841 gctttttgtt cttcagttgg ttccaatcga tactcaacac ccatctgagg catgataatg
     3901 gtgatatctg cttccttctc tgcccgttcg acttcagcct ttatcttatc ttcgtttaaa
     3961 tctgaaagat aacgattata gtcttcctga aaaatatact gctcaattcc attgaaacca
     4021 taggaatagg ctaacaatgc aaccttgata gcattcactt ccttaatgac cagcggagcc
     4081 tgatcacgtg gttcgtgcgt ataaactccg attggagtga ttccagcttt ctcaataata
     4141 tcggccattg aaataactcc ctcaatttgc gaatccaaaa tatgattatg agctaaatcc
     4201 agcacatgat aacctgcttc cttaatagca tccataactt caacaggagc attaaagaga
     4261 agataacccg ctaaataatg atccttatta atggttcctt caaaatcacc aatagctaaa
     4321 tctgcttgct tgagccaagg agtcacatac tcaaaatttt catgaaagtc ataggtaccg
     4381 tcttcttttt tagctgaaaa gaaaagtcca tcgtggtaga gcaaatcccc gtgggccata
     4441 attctggcag ttttttcctc ttcctactcc tgagactttt gcttagtccc ctcttgagaa
     4501 atagtatctc gtttttgact agtcaaggga ttccttggaa gctttcaaac aacaagacca
     4561 agcccattga taaaccaact gctagcaaga gtatcgccac aaatccctta ttgctccact
     4621 tgcgataact cctaaaaagt ttaccaagcc cttcataaaa cgaaaagcta aaccaccctg
     4681 atttcgattt tgtcttcttt gtatcttcat tctccctact ttcttatgca agccttttct
     4741 ttttattata tcacagataa gtatttcttt cacaattgaa ttgaacttcc catctatttt
     4801 ctataaatcc taaatgccat aatgctttca attcttgtca ttttgtgata tcatgtagag
     4861 gtaatgaaag gaaatgaact aatccacagt ggcttattcc aagtatacca cttgggcttt
     4921 ggcagtagtt aactgcacta aacataatat aaggagagaa aatgtctgca atagaacgta
     4981 ttacaaaagc tgctcactta attgatatga acgatattat ccgtgaaggg aatcctactc
     5041 tacgcgcgat tgctgaggaa gtcactttcc ccctatctga ccaggaaatc atcctaggcg
     5101 aaaagatgat gcaattcctt aaacattccc aagatcctgt catggctgaa aaaatgggac
     5161 tccgcggtgg tgttggactg gctgctcccc agttagatat ctcaaaacgc attatcgctg
     5221 ttttggtacc taatattgtt gaagaaggcg aaactccaca ggaagcctac gatttggaag
     5281 gcattatgta caatccaaaa atcgtctctc actctgttca agatgctgct cttggcgaag
     5341 gagaaggttg cctgtctgtt gaccgtaacg tgcctggcta tgttgttcgc catgcccgcg
     5401 ttactgttga ctactttgac aaagatggag aaaaacaccg tatcaaactc aaaggctaca
     5461 actccattgt tgttcagcat gaaattgacc acattaacgg tatcatgttt tacgatcgca
     5521 tcaatgaaaa agacccattt gcagttaaag atggtttact gattcttgaa taaagaaaat
     5581 cccgttgcaa gacggggttt tgtgttataa tagaggcatg aaaacaaatg atattgtcta
     5641 tggtgtccac gccgttaccg aagccctcct tgcaaataca ggaaacaaac tctacctcca
     5701 agaagatctc cgaggtaaga atgttgagaa agtcaaggaa ctagctacag aaaagaaggt
     5761 gtccatttct tggacatcaa aaaaatctct ctctgagatg actgaaggtg ctgttcatca
     5821 aggttttgtt ctacgagtgt ctgaatttgc ctatagcgag ctagattaca tccttgcaaa
     5881 aacacgccaa gaagaaaatc cacttctatt gattctagat ggtctaaccg atccccataa
     5941 tctgggttct atcttgcgaa cagccgatgc gaccaatgtt tcaggtgtca tcattcccaa
     6001 gcaccgtgct gtcggagtaa ctcctgtcgt tgccaaaaca gccacaggtg ctattgaaca
     6061 cgttccaatt gcccgagtga caaacctcag tcaaacctta gataaactca aggatgaagg
     6121 cttctggacc tttggaacgg atatgaacgg tactccttgc cacaagtgga atacaaaagg
     6181 gaaaatcgcc ctcatcatcg gaaatgaagg aaaaggcatc tctagcaaca tcaaaaaaca
     6241 ggtcgatgaa atgattacca ttccgatgaa tggacatgtt caaagcctca atgccagtgt
     6301 tgctgcagcc attctcatgt acgaagtttt tctaaataga ctataaaaaa gtttccagtc
     6361 atctgattgg aaactttttt atgattaact atgttctgtg ataaacttgt aggcttcttg
     6421 accagcgata gctccatctc caactgctgt tgttacttgg cgaaggtctt tcaagcgaac
     6481 atctccaact gcaaagatac cgtcgactgc agtcttcatg tggctatctg tcacaatcca
     6541 gcctgcctga tcttggatat tcaattcttt aacaaaatcg ctaagagggt ccaaaccaac
     6601 atagataaag acaccaccga aggcttgctc tgtcacttga cctgttttca cattttcaaa
     6661 aacgacagat tctactctgt tttcaccctt gatttccttc actacagaat cccagataaa
     6721 gctgattttt tcattcgcaa aggcgcgatc ttgtaaaacc ttttgggcac gaagttggtc
     6781 acgacggtga acaatggtaa cagtcttagc aaaacgagtc aagaagaggg cttcttcaac
     6841 agctgaatct ccaccaccaa ctaccaataa atcttggtca cggaagaaag caccatcaca
     6901 cacagcacag taagaaacac cacgactgtt cagttcttct tctccaggca ctcccaaagg
     6961 acggtgttta gaaccagttg ctacgataac tgtacgtgtt tcatatgttt ggtcatcagt
     7021 catcactttc ttaaaatcac catggtcttc gacattttca acataaccat aaatgtgctc
     7081 aacaccaaga ttttcaagtg gttcaaacat cttttcagcc aattcaggtc cactaatatt
     7141 agcgtatcct gggtaatttt cgatatcaga tgtattattc atctgaccac ctggcagacc
     7201 accttcaatc aaagctactt ttagattgct tcgagcagca tacaaggccg cagtcatccc
     7261 tgcaggtcca gcaccgataa taatagtatc gtacatatag attccttctt tcttgttgta
     7321 actatcttta ttctaactct ttcttgtcaa tcacgcaagg attatgcctt gaaaacaaga
     7381 attaaactca taaccgcaaa ggataatact gggacaacca agactccaat cataatcata
     7441 tcatagagat gggcttggcg agccctggct gtcttatcaa ctcccgacat ggctctcaga
     7501 ccaatccaaa tccctaaaaa aatcagaaca aggatggtgg tcaagatcaa actctcgaaa
     7561 tataaagaaa atagttgcag tagcatgatt tctctcattt ctatcttttt taaagagtaa
     7621 actcagctag tccaactaac tgagttttcc tttatctatt atatcaaata taagtccgtt
     7681 tgtaactagc gaagaattct tttgtccgct cttctttagg ggtgtggata atctcatccg
     7741 gagttccaga ctcgatgatt ttccccttat ctaataagag aattttatcc gcaacttggg
     7801 ctacaaagga catgtcatga ctgaccaaaa tcatggtctg acctgactta gcagcatctg
     7861 caatagactt ttctacttca ccgaccaatt ctgggtcaag ggctgaagtt ggttcgtcta
     7921 agagcaaaac atctggtttc atagcaagcg cacgcgctag ggcaacccgt tgcttctgtc
     7981 cacctgataa atggcgagga taatggtttt cacggtccga aagcccaacc ttagccaact
     8041 cttccttggc aatcttagtc gcttcttggt cagataattt cttgacaaca accaagcctt
     8101 ctttcacatt atcaagtgct gttcgacgtt caaataaatt aaactgttgg aaaaccatag
     8161 acaacttacg gcgtagggca aggatttctt cttgagtgat tttagaaaaa tcaactgaaa
     8221 aaccatcaat ctgaatagag ccactgtcag gtgtttcaag ataattgaga ctgcgaagaa
     8281 aggttgattt tccagctcct gaagaaccaa tcaaggctac aacttcccct ttttgaatat
     8341 ccaagttcag atgatccaag acagtctgtc ctgaaaagga tttgcttaaa ttcgaaatct
     8401 taatcattaa cgaaggtctc ctttcacatc tgtttgcact gtatcaggtg cagaaatagc
     8461 catttttctc tcgatgaaac gaccgaggct ttcaattccg atattgacta cccaataaac
     8521 aagggcaaca gagatgaagc gttcaaaata gcgataatca gctccaccta gaatctgagc
     8581 ttgggcaaag acttccacaa cacccgcact aaaagctaga gatgttccct tggtcaaacc
     8641 gatgagggaa ttaatcaagg ttggagtagc taccaccgct gcattaggaa taatcactcg
     8701 tcgataaact tgcgctcggg tcatacccag actgcgcgcc gcctcaatct caccaggatt
     8761 aactgagaga atggctgcac gaatggtttc actagcataa gctgcctcat taaaggcaaa
     8821 agcgacaatc gcaaaagctg cagctggaat cgcattgata ttgagaccag ttccccattg
     8881 ctgattgagg gctttcaaag ccaaagggat tccgtagtag gtcaacatga gttgcaccaa
     8941 aatcggtgtc ccttttaaga aactaacaaa gaaggcctgc aagggatata aaatcttgac
     9001 acgattgatc ttcacaatgg caaaaagaag cgccaaaacc aagccaaaaa gggcaccacc
     9061 aattgtcaac ataattgttg ttggaagttg ttggacaatt ctagggattc catcaaagac
     9121 cgaacgtagg ctaaacagct tgccatccgg aatcaattgc atcaagtttt ggtaccaatc
     9181 tgatgctaaa atcgttgtaa cattcataaa aacatcctct tctgataatg attcattcta
     9241 ccatgcttct gccgtcaatg aaattatttg ctacggtcag atacagactt tgtctaccta
     9301 ctagtttatc atactttgaa acagggaagt tatatatttt atctatcaaa gagatagata
     9361 aaaactattt cagtcccagt tcttcataag attttcgata accgatttgc ttaacagttc
     9421 cattttctac taaaatgggc cgttttaaca acataccgtc acttgctagc aactcagccg
     9481 cttcttggtt tgacaaactt cctaccttat cttttagccc taattcacgg tatttgatac
     9541 cactggtgtt gaaaaattgc ttcaattcaa atcctgaggt ttctagccaa ttcaaaatga
     9601 cttcttggct aggtgtttct tccacgatat ggacggcttt atagtccaca cctaattgat
     9661 ttaattcttg ttttgctttt ttacaagttg aacatttggg gtattcgata aattctaaca
     9721 tgtgcttctc cttctatttg tttatatctt taaaatctac gccttcatgc tccaagagcc
     9781 aagctttctt ttccactcct gcagcatagc ctgtcagacg cttgcctgct cccaacacac
     9841 gatgacaagg tactaggata gaccaaggat tgcgtcccac tgctccacca attgcttgag
     9901 cagaagccac ttgcaggtct tgagcaattt gtccataggt cactgtctga ccataaggaa
     9961 tgccctgtaa atagtcccaa actctctttt caaaattcgt tccgattggc gccaagagca
    10021 agttggataa atcctgaggc ttgcctttaa agtaatcatc taagcaagca ataactgggt
    10081 ctaaaatagg atgactaaca acttcttcta tcgtttcatc tcctagtccc ctctcaaaat
    10141 gcttctgctc ctgaacccaa attccataca aataatggtc atcagctaca agtgatagaa
    10201 taccaattgg tgaggagtag agtattttta catacttctt ttgcattatt tctttaattt
    10261 ttgataggcc aagcgttcgc catctactgg caccttacca acttgtttaa aaccaagttt
    10321 ttcaaaaata tgttgcataa ccttgttttc agcatgcgta tctgagcgaa aatcaagata
    10381 atcaaaacct tcaatcaagc cctctaagaa ggtttgagca actccttttc cctgcacatc
    10441 tgctgccaca gcaatacggt gaaagactag atactctgac tctccagctt gccagtttcc
    10501 ctcataaata gcttcatagg cctcctctgg actcttggtc acagcagcat aggctagcag
    10561 ttctccctct tccaaggcta cgtaggcttg ccctgagatg atatcatcga taataacatc
    10621 tgcatttgga tagccatttt gccactggtc actaccagca tctgctaaac attttttggc
    10681 atcctccatc acctgcatga tggcatctac ttcatttgga aaagctaaac gaatttccat
    10741 cttttttcct catttctgac tagtttctcc catcatagca taatttaagt atttctacaa
    10801 gcagttaaaa cttgactttg tgtttttgtt attttataat ctagttatca aaactatata
    10861 aaaaggagtt gaaaatgaaa actacctttt cctacccaaa atgggcagaa attccaaaca
    10921 ttgacctcta tctggaccag gttttgctct atgtcaatca ggtctgcgcc cctatctctc
    10981 ctaataaaga caagggccta acagcatcta tggtcaacaa ttatgtgaaa aatggttacc
    11041 tgacaaagcc tgacaaaaaa aaataccaac gccaacagat tgcccgtttg attgctatca
    11101 caaccctcaa gtctgtattt tctattcaag aaatagccca gacacttaat actctacaaa
    11161 ctcaagcaag ttcagaccaa ctctacgatg cttttgtgga ctacatgaac caaggaattg
    11221 acccagctaa ccctattatc caaaccagct gccaaaccgt caaactctat catcaaactc
    11281 tagacttaat cgatcatact caagaggagg taatccaatg aacactagtc ttaaactcag
    11341 caaacaactc agttttggag aggagattgc taatagcgtg acccatgctg tgggtgcagt
    11401 catcatgctt atcttgctgc ctatttcatc catctatagt tatgaagcac acggattttt
    11461 atcctctatc ggcgtttcca ttttcgtcat cagtctcttt ctcatgttcc tatcatccac
    11521 catttatcac tctatggcct atggttcgac ccacaaatat gttttgcgaa tcattgacca
    11581 ttctatgatt tacgttgcca ttgccggctc atacacgccc gttgtcttga ccttgatgaa
    11641 taactggttt ggctatctga ttattgtcat ccaatgggga acgaccatct ttggtattct
    11701 ctataaaatc tttgctaaaa aggtcaatga gaaatttagc cttgctcttt acctgattat
    11761 gggctggttg gttctggcta tcattcctgc cattatcagt caaacgacac ccgttttctg
    11821 gagtctcatg gtaactggcg gactctgtta tacagttgga gctggatttt atgccaagaa
    11881 aaaaccttat ttccacatga tttggcatct ctttatccta gctgcgtccg cacttcaata
    11941 catcgctatt gtttattaca tgtaaaaaag ttgagaaatt caatctcaac ttttttcttt
    12001 acacatattg ataaagtact ggtgcaagcg cacatcatca gtcaattctg gatgaaaaga
    12061 acttaccaac atattttttt cttgggctgc aacaatttga ttgttcactg ttgctagaat
    12121 ttctacaccc tcaccaacac tactgataat cggaccacgg ataaaggtca ttggaatctt
    12181 gccaactccc ttacattctg cttccgtgta gaaacttcct aattggcgcc cataagcatt
    12241 acgctcgacc accatatcca tagttcctag atgactctct ttctgagaag tgatttcctt
    12301 agccagcaaa attaagcccg cacaggtccc aaacactggt aagccagata gaatggcttc
    12361 tcggatggga agtagcatgt tctggtcacg taagagcttg cccatggttg tagactcacc
    12421 accaggcaaa atcaaacccg acaagtcact ctgatcttgc tgaaaatcat ctagatttct
    12481 gagttctaca ctctcgacac ctaattgatc tagcactttt gcatgttctg caaaggcccc
    12541 ttgcaaggcc aatattccaa ttttcatcta ttttcctcgt tcagccatga gaatttggat
    12601 ttcattttca ttaataccaa ccatggcttc tcctaaatct tcagagattt gagctaggat
    12661 ttgaggatta cggaagttag tcacagcctt aacaatggca ctcgctcgtt taacaggatc
    12721 tcctgacttg aaaatacctg aaccgacaaa gaccccctct gcccctaatt gcatcattaa
    12781 cgcagcatct gctggcgttg caacacctcc agcagcgaaa tttacaactg gcaattttcc
    12841 atgttcatga acatattgga ccaattctac agggacttgc aaatccttgg cagcaacata
    12901 aagctcgtcc tcacgtaagt tttgaatgcg gcgaatttcc tgattcatca tacgcatatg
    12961 acgaacagct tggacgatat cccctgtccc tggttctcct ttggtacgaa tcatggaagc
    13021 accttcagcg atacgacgca aggcttcacc caaatcctta gcaccacaga caaaaggaac
    13081 ttggaattct ttcttgtcca catggaaacg gtcatcagct ggagatagaa cttcactctc
    13141 gtcgatataa tcaatttcaa tagcctctaa aatctgagct tcaacaaaat gcccgattct
    13201 gaccttagcc attactggaa tactaaccgc ttcttggatt tccttaatca tctttgggtc
    13261 gctcatgcgg gaaactcctc cagctgcacg aatatcagcc ggaattcgtt ccaaggccat
    13321 cacagctgcc gcaccagcag cttctgcgat acgagcctgt tcaggattct gcacatccat
    13381 aataacacca cccttgagca tctgtgccaa gtttttattt agttcataac gattttcagt
    13441 catttgtttt atcctcatca tttgtattgg tagctacctt ttcattttaa gtcagaacag
    13501 aatgaagcac aagataaaaa aactataatt gtatggggac agattggcta aaaactatct
    13561 gaccttaact taaggccaga tagctcattc atttttattt ttcagccgta agggcagcca
    13621 ttgtgatgta gttgtatggt ttattgaagt gtggcaagaa gaagaggtct gtcaatgcca
    13681 atttatcaat tgtcacatgc tcttggatag caagtgagaa catgtggatt cccatgctaa
    13741 ttgcaatatc atgtgaaacc atttgggcac caagaatttc acggctatct ttgtcaaaga
    13801 caatcttaat tgctacttca tggttgtcat gtttcatgaa ttctggtttt tgaagatcgt
    13861 taaagcctgt ttcagttgcg ttgtaaccag cagctttcgc tttttcaaga gtcaaaccag
    13921 ttgaaaccat gtgaagaccg tagattgaga taccatttga accttgaaca ccgattcctt
    13981 ccaattcatg tccacaagcg ttgtaggcac caacgatacc agtgcgaaca gcatttgaag
    14041 caagagcgat atagcttgta tctttacgag cattgtcata aacagtcgca cagtcaccaa
    14101 cagcgtaaac gcctgggata gatgtttctt gtttcttgtc tacaaggaag gcaccgttgc
    14161 ggaagagttc gatcttacca tcagcaaggg ctgtgtttgg acggaaacca actgcaagga
    14221 taaccatatc cacgtcaaag ctttctttgt cagtaatcaa gcgttcaact ttaccatcac
    14281 cttcgattgc tttaacagtt tgacctagag ccaagcggat gttgtgatct tccaagttct
    14341 tcgccatcat ttgtgtgaag tctttgtcat agtaaccgtt caagacagta tcaacgatat
    14401 caacaaggac aacttctttt ccaagacgtt caaaggcttc agcaagttca acaccgatgt
    14461 aaccaccacc aacaacggcg atacggtcga gatgttggct cttgtcagaa agtttattga
    14521 taacttcttc agcattttgg tacaatttca cgaattgtac gttttcaaga gttgctttaa
    14581 attcgcggtt tcctttaaca atttcaacac cttcgattgg tggcaagatt ggtgtagaac
    14641 ctgtagcgaa aatcaatttc tcgtatgatt ctttgtgttc ttttccttca acttctgctg
    14701 taaccacttt gttatcatag tcgattgaaa gaactggtga attcatataa accttagcac
    14761 ctttagcttc taatttgtct ttatcagaat agaacaagcc ttcagcaccg tcaatttgtt
    14821 caccaatcca aagagccatt ccacatccta ggaaagagat gttagagttt tggtcaaata
    14881 caacaatttc gttctcattt ccaaaattat ccaacatggt attgatacat gctgtaccag
    14941 cgtggttagc accgactaca acgattttac tcataaaaaa tttcctgcct tcattctaaa
    15001 tttactttac tagtatacca tttaccgtta gcgtttacaa gggggggact attatttcag
    15061 cattttttat caaaaataat tccccattta tcctgttttc aagcctcatg aattttatga
    15121 gcatatttct tgactttttc caaattttgt ttgtgaaaat tctaacttta tggtattata
    15181 ataacatgaa aataaaatgt aagggcttca atttagatga ttatttgata ttgcaactta
    15241 ttttctataa gagaaaggag ttggtagctt gactcttcgt tcacattctg aacggctaat
    15301 ggggactact atcactattt cattagtgga tgagcaagcc gatatctttc tccaaaaatc
    15361 ctttgacttg ctcaaagagc ttgaataccg cttcaacgcc aatagtcaag aatctgagtt
    15421 gatggaaatc aattatcaag caggaatatc ccccgtcaca gttcatccag acctgtttga
    15481 gctgatttca cttgggttag agcatagcct agcgctctct agccacctca atatcagcat
    15541 tggtccctta attcaaacct ggcgtatcgg tttttcagat gccaaggtcg cccagcctca
    15601 agaaattgaa tcggtgctgc ctctaatcaa tcctcatggt atcgagttag attcttctac
    15661 ttctactgtg tttttaaaac agaaaggaat gaagatcgat ctaggttgtt tagccaaagg
    15721 atacagtgcg gataaggttg cccaatttct taggaaagag ggggtgactt ctgccttgat
    15781 caatctggga gggaatatcc tgaccattgg aaaaaatcag gcaagagggg ataacccatg
    15841 gcaaatcggg attcaagacc cagccaatcc taggggaaat catttaatga ccatccctgt
    15901 tgtcaataaa tctgtcgtga cttcaggcat ttatgaacgt cacctgaccg tcgatggaca
    15961 agattaccat cacatttttg acagtcaaac aggatatcct gttgaaacgg aactagcgag
    16021 tctaacaatc atctctgata aatcagtcga tggcgaaatc tggacgactc gactctttgg
    16081 agaaagacct gcttctatcc tctggcaagt cgaaagtttg gagggcatcg aagtcatcct
    16141 catcgataaa gaaggccacc taagctgttc ttcaggaatt cctactctat agagacaact
    16201 gtttcaatgg aattttaaaa ttgtattatg taaaaagaga aaggaaatat catgttaaaa
    16261 cttattgcta ttgttggaac aaattcaaaa cgttctacaa accgtcaatt gcttcaatac
    16321 atgcaaaaac actttgctga caaagctgaa attgaacttg ttgaaatcaa gaccattcct
    16381 gtcttcaaca aaccagctga caagcaagta cctgctgaaa tattggaaat tgctgctaaa
    16441 atcgaagagg cagatggcgt tattatcggt actcctgagt atgatcactc tattccagct
    16501 gttttgatga gcgctcttgc ttggttgtct tatggtattt acccactttt gaacaaacca
    16561 atcatgatta caggtgcttc ttacggtacg cttggttcat ctcgtgccca attgcaactt
    16621 cgtcaaatct tgaatgctcc tgaaatcaag gcaaatgttc ttccagatga attcttgctc
    16681 tcacactctc ttcaagcatt taacccaagt ggcgacttgg ttgaccttga tgttatcaag
    16741 aaattggatg ccatctttga tgacttccgt atcttcgtga agattactga gaaattgcgc
    16801 aatgcgcaag aattgcttcg caaagatgct gaagactttg actgggaaaa tttgtaagat
    16861 aggagaccga aagaatgaaa tttgttggac ttgttggatc aaactacgat caatcatata
    16921 accgcaaact cttggaattt atccgtcgca atttcaaatt caaatttgaa ttagaagtac
    16981 ttgaaatcga cgaagttcca atgtttaacc aagacgaaaa atgggacgaa agcttccaat
    17041 tgcgtttctt gtataacaag attacacgtg ctgatggtgt cattatcgcc actcctgagc
    17101 acaaccacac tatctcagct tcactcaaat ctgtacttga atggctttca tacgaagttc
    17161 atccatttga aaacaagcct gttatgattg tgggtgcatc atactatgac caaggaacat
    17221 cacgtgctca agttcacctt cgtaaaatcc ttgatgctcc aggtgttaat gcctacacgc
    17281 ttccaggtaa cgaattcctt cttggtaaag ctaaggaagc ttttgataac aacggaaata
    17341 tcaccaacga aggcactgtt aaattccttg aaacttgctt agataacttt gttaaatacg
    17401 taggagtcgt ttcgaaattg aaaaaaccaa aaccaatcga atcagaagac ttggattgtg
    17461 gaaaaccaat cgctacaacc atcacagaag ttgaccctga cgatccagaa tgggtagaaa
    17521 aagttgcagc aatcactggt gctgtttctg gtgataccta tgtcaaattg gaccacggta
    17581 tcctaacagt taaccaaatt gatatgttct tgaaagctat gccatttgaa ttgacatacg
    17641 ctgacgacaa caaccaattc ctctactaca acaacgctca ccaagatcca gacactatgt
    17701 tcgctaaacg tgtaccacct caatcaggta gccgtatgtc gactgttcat ggttctcttc
    17761 caccagcacg tatgaagaac gtagagtggg ttatcggaac acttcgcaac ggaaaccaag
    17821 aatacgtccg tacgatcgtt ccaggttctc ctgcaggtgt tatcaacact cacaactacc
    17881 aagcaatgta ctatcctgat ggatcatacg ctggtatcaa tgaaatcgtc tttaacttcc
    17941 aaccatggct tgactggtac ctaaaagaga ctggtcaacg tttggtgggt ggtagcggac
    18001 catttgctcc tgctgccgga ggtcatggag acgccgatgc tacttctggc gcttctgatt
    18061 caggggatgc tggaggccac ggtggtgacg cagacgctac agccggtgca agtta
//
LOCUS       DAVBCG010000030        17816 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBCG010000030 DAVBCG010000000
VERSION     DAVBCG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.030"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            119..778
                     /locus_tag="VQ495_000841"
     CDS             119..778
                     /locus_tag="VQ495_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2530821.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            803..1870
                     /locus_tag="VQ495_000842"
     CDS             803..1870
                     /locus_tag="VQ495_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2530822.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            1974..2984
                     /locus_tag="VQ495_000843"
     CDS             1974..2984
                     /locus_tag="VQ495_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530823.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            2996..4687
                     /locus_tag="VQ495_000844"
     CDS             2996..4687
                     /locus_tag="VQ495_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2530824.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            4690..5400
                     /locus_tag="VQ495_000845"
     CDS             4690..5400
                     /locus_tag="VQ495_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2530825.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            5496..5864
                     /locus_tag="VQ495_000846"
     CDS             5496..5864
                     /locus_tag="VQ495_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530826.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            5972..6973
                     /locus_tag="VQ495_000847"
     CDS             5972..6973
                     /locus_tag="VQ495_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2530827.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            7578..7655
                     /locus_tag="VQ495_000848"
                     /pseudo
     CDS             7578..7655
                     /locus_tag="VQ495_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            8156..9517
                     /gene="trpE"
                     /locus_tag="VQ495_000849"
     CDS             8156..9517
                     /gene="trpE"
                     /locus_tag="VQ495_000849"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2530828.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            9514..10080
                     /locus_tag="VQ495_000850"
     CDS             9514..10080
                     /locus_tag="VQ495_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2530829.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            10091..11095
                     /gene="trpD"
                     /locus_tag="VQ495_000851"
     CDS             10091..11095
                     /gene="trpD"
                     /locus_tag="VQ495_000851"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2530830.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            11092..11859
                     /gene="trpC"
                     /locus_tag="VQ495_000852"
     CDS             11092..11859
                     /gene="trpC"
                     /locus_tag="VQ495_000852"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2530831.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            11846..12445
                     /locus_tag="VQ495_000853"
     CDS             11846..12445
                     /locus_tag="VQ495_000853"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2530832.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            12423..13646
                     /gene="trpB"
                     /locus_tag="VQ495_000854"
     CDS             12423..13646
                     /gene="trpB"
                     /locus_tag="VQ495_000854"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2530833.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            13639..14415
                     /locus_tag="VQ495_000855"
     CDS             13639..14415
                     /locus_tag="VQ495_000855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2530834.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(14387..14488)
                     /locus_tag="VQ495_000856"
                     /pseudo
     CDS             complement(14387..14488)
                     /locus_tag="VQ495_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            14717..14857
                     /locus_tag="VQ495_000857"
     CDS             14717..14857
                     /locus_tag="VQ495_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530835.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            14894..15448
                     /locus_tag="VQ495_000858"
     CDS             14894..15448
                     /locus_tag="VQ495_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530836.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            15463..16614
                     /locus_tag="VQ495_000859"
     CDS             15463..16614
                     /locus_tag="VQ495_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2530837.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGNRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(16666..17325)
                     /locus_tag="VQ495_000860"
     CDS             complement(16666..17325)
                     /locus_tag="VQ495_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2530838.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            17395..>17816
                     /locus_tag="VQ495_000861"
     CDS             17395..>17816
                     /locus_tag="VQ495_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2530839.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVR"
BASE COUNT         5726 a         2891 c         3844 g         5355 t
ORIGIN      
        1 cttttaatga aagataaatt aaaaaaaatg tttgacagcg cttgacaaag gaaacggtgt
       61 cattgtataa tgtaaacaca atgacaccga tgtcaaatat aaaaaagaag gtctttatat
      121 gaaaaactta acaaaaatta agttcaaaga aaatggggaa tttaatcatt tccctgggaa
      181 tacagttgta gcaaatcttt atactaaaca agatttgatg gaagttgttg atattattca
      241 atcacgttat agagaattac catttatcga taagtttact ttaactccaa ggaattctat
      301 tcacatgact gtaattgaat tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag
      361 taatcttcct ctagatacac ctttacagga aatacatgat tactttgcta aacaacttga
      421 aatttttcca ttgttggatg aagaaattca tatgcgtata actgaaatgg gaaaacaaaa
      481 catactagtt gaacctgcag atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta
      541 tgtctcagaa aaagcaggtg tttgtttccc taatcatgat agatatcaat tccatatttc
      601 aattgggtat cttcggattc ctctaaccga agaggaggaa gaagagttta ctaaagtcag
      661 agcagaatta actgaaattt tattagagaa gattccaact attactgtaa accgtattga
      721 ctatactgta tttgaagata tgaggcaatt tgttccatat cacgaaaaat ttaaataaaa
      781 taataaaaaa ggagaactta tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac
      841 gggagttgct cttactacac ttgctgcttg ctctggaggt tcaaattcta cgactgcttc
      901 ttcatctgaa gaaaaagctg ataaaagtca agaattagtt atctattcga actcagtctc
      961 aaatggtcgt ggtgattggt taactgctaa agcaaaagaa gctggtttta atataaaaat
     1021 ggttgatatc gctggcgctc aattagcaga ccgtgttatt gctgagaaga ataatgcagt
     1081 tgcagatatg gtatttggaa ttggtgctgt tgactcaaat aaaattagag atcaaaaatt
     1141 actagtacag tacaagccta aatggttaga taaaattgat caatctttat cagataaaga
     1201 taattattat aatcctgtga ttgttcaacc attagtttta attggggcgc ctgatgtaaa
     1261 agaaatgcct aaagattgga ctgaattagg tagtaagtat aaaggtaaat attcaattta
     1321 tggtcttcaa ggtggtacag gacgggcaat tctagcaagt atcttagttc gttaccttga
     1381 tgaaaaagga gatttaggcg tatcagaaaa aggatgggaa gttgcaaaag aatactttgc
     1441 aaatgcctat actcttcaaa aaggtgaaag ctcagttgtt aaagcgttag ataaagaaag
     1501 cccaattcaa tacggtatga tctggggttc tggagcgtta attggacaaa aagaacaaaa
     1561 tgttgaattc aaagttatga ctcctgagat tggtgtacca tttgtaactg aacaaactat
     1621 ggttttaagc actagtaaaa aacaagcgtt agctaaagaa tttattgatt ggtttggtca
     1681 atcagaaatt caagtagaat atagtaaaaa atttggatct attcctgcca ataaagatgc
     1741 cctaaaagag ttacctgaag atacgaaaaa atttgttgat caagtgaaac cacaaaatat
     1801 tgactgggaa gctgttggaa agcatttgga tgaatgggta gaaaaagctg aattagaata
     1861 cgtaaaataa gaaataagag ttatttttta tgtccaagtc tctctattat ttctaaattt
     1921 aaggaagaaa taaagagagg cttttggttt tagtaataga gggagttagt gagatgatta
     1981 aatttgataa tattcaaatt aaatatggtg attttgttgc aattgataat ctgaatttag
     2041 atatacatga aggggaattc tttacatttc ttgggccttc aggatgtggt aaatcaacta
     2101 ctttgagagc attggtaggt tttttagatc catcatcagg aagtattgaa gttaatggaa
     2161 cagatgtcac tcatttggaa cctgaaaagc gtggaattgg tattgtattt caatcttatg
     2221 cgctatttcc aactatgact gtttttgata atattgcatt tggtttaaaa gttaagaagg
     2281 tagctccaga tgttattaaa gctaaagtat cagcagtggc agcaaaaatt aagatctctg
     2341 atcaacagtt acagcgtaat gtatcagaat tatctggggg tcaacaacaa agggtagcat
     2401 tggctcgtgc tctggttctt gaacctaaaa ttctttgtct agatgaacca ttgtcaaacc
     2461 ttgacgcaaa attacgtgta gatttgagaa aagagttgaa aagacttcaa aaagagttag
     2521 gtattactac tttatatgtt actcatgatc aagaggaagc cttgacttta tctgatagaa
     2581 ttgcagtctt taacaatgga tacatcgaac aggtcggtac accagtagag atttatcata
     2641 attctcaaac tgaatttgta tgtgatttta ttggagatat taatgttttg accgatgaaa
     2701 cagtccacga agtattactg aaaaatacaa gcgttttctt agaggataaa aaaggttaca
     2761 ttcgattaga gaaagttcga ttcaatcgtg aaactgaaca agattttatt ctaaaaggga
     2821 caattattga tgttgagttt tctggagtta caattcacta tacaataaaa gtttctgaaa
     2881 gtcagattct taatgtaaca agtattgata gtcaggctgc tattagatct gtcggagaaa
     2941 gtgtggaatt atttatcaca ccatcagacg ttctgcaatt ttaaggaggt gcagtatgcg
     3001 tcataaatta aatttaaaag attggcttat tcgtttaggg ttaatctggt tcttagtaac
     3061 atttattatt tatccaaact ttgatctagt agtgaatgta tttgtaaaag gaggagaatt
     3121 ttcccttgat gctgtacatc gtgttctaaa atctcagagg gcacttcaga gtattatgaa
     3181 cagttttaag ttagcatttt cactcattat tacagttaat gtcgtaggta ttctttgtgt
     3241 tctatttaca gagtactttg atattaaagg tgctaaaatt ttaaaattag gttatatgac
     3301 ctctttaatt tatggaggag tggttttagc gactggatat aaatttgtct atggtcctta
     3361 tggattgatt acaaaatttt tacaaaatgt tatcccttct ttagactcta actggtttat
     3421 tgggtatggt gcagtcttat tcattatgac attttcagga actgctaatc atacattgtt
     3481 tttaacaaat acaattcgaa gcgttgacta tcacactatt gaggctgctc gaaatatggg
     3541 agcaaaacca tttactgttt tccgaaaagt agtgttacca accttaattc caactctatt
     3601 tgcacttact attatggttt ttcttagtgg tttatctgca gtagcagcac ccatgattgt
     3661 tggtggtaaa gaatttcaaa ctataaatcc aatgattatt acatttgcag ggatggggaa
     3721 ttctcgtgat ttagctgcct tacttgcaat tattttaggt attgcaacta caattttgct
     3781 tactatcatg aataagatag aaaaaggtgg aaattatatt tctatctcta agactaaagc
     3841 gcctcttaaa aaacaaaaaa ttgcgtctaa gccttggaat atcattgctc acattgtagc
     3901 atatggattg ttcacagttt tcatgcttcc actaattttt atagtattat actcatttac
     3961 agatccagtt gcaattcaaa caggtaactt aacattatca aactttactt tagaaaatta
     4021 tcgcttattc tttagtaata gtgcggcatt ctctccattc ttggtcagct ttatttattc
     4081 tattattgct gcgacaacag caacaattct cgcagttgta tttgctcgtg ttgtcagaaa
     4141 acataaatct cgttttgatt tcttatttga atatggtgct ctacttcctt ggttactacc
     4201 aagtacactt ttagcagtaa gtttattatt tacttttaat cagccacaat ttcttgtctt
     4261 gaatcagatt ttggtaggta gtttggtaat tctacttatt gcatatatag ttgtaaaaat
     4321 cccattttct tatagaatgg tacgtgctat tttatttagt gttgatgatg agatggaaga
     4381 tgcagcaaga agtatgggtg cttcaccttt ttatactatg atgaaggtta tcattccatt
     4441 tattttaccg gttgttctct ctgttattgc tttaaacttt aactctttat taactgactt
     4501 cgacttatct gtattccttt accaccccct agctcaacca ttaggtatta cgattcgatc
     4561 tgcaggtgat gaaacagcaa catctaatgc acaagctctg gtatttgttt atacaattgt
     4621 tctgatgatt atttctggaa cggtattata cttcacacaa agaccggggc gtaaagtaag
     4681 gaaataatca tgacagccac tagtcttggg ttatcaaata ttgaaatagt tgtcaggatt
     4741 gttttatcag tagtcattgg tagtataatt ggtttagaga gagggagcaa atcccagcct
     4801 gcaggcatcc gaacttatag tattgtttgt ctagctgcat gtttgattat gatgacgaat
     4861 gaatacgtat cttataaatt tgggacagga gatcctacac gattaggagc tcaagttata
     4921 tcaggtgtgg gttttctagg cgctggaacg attcttatta cagataaaaa gaaaattaca
     4981 ggtctgacaa ctgcagcagg catttgggct tcggcaggaa ttggattagc tattggagta
     5041 ggtttttatg agggagctct tttagtagcc atttctgttt ggggtgtgat atccatgttc
     5101 caaccactaa aaaaatatct acaaaatcgt tctaaaatga ttgaattgta tatagtagtt
     5161 aaatctacag aagcctataa tcgtgtattg gtttattgtg cagaaaatgg tatcagaatg
     5221 accgattcaa gaacagcgtt tggagatgtt aattctgaca gaattgaata ctttgatgtt
     5281 ccagataaaa aaatagcgtc atttattact ctggaattat caggtagatt tgaacatctt
     5341 cgactgatgg aagaaattgc taatattgta ggtgttattt atgtcgaaga aattagctga
     5401 aatttagtaa acaaagggac agatgttttt gtccttttgt tttttagata ttttaatcat
     5461 ctaatttttg agtagatttt tgctcagagg tttttatgaa agattataaa atcaattttg
     5521 atttaggtaa aatagaatat tttgataata attgtttgat acaagtatac aaatttattt
     5581 ctttttatga tatttgtgaa atggtgtttg cctttcattt acctcctgat gagttgataa
     5641 caaatgttat ctttaaagaa aaaattaact ctatgttaaa atgttacatt gacaggctat
     5701 tatatgtttt tataaatcct acacatttta ctgaaaaagt taatctacaa ttttatggta
     5761 gttttttctc atatgaattt atttgtcgtg aagttggaaa cattcttaaa aataaaggtg
     5821 tgaaatgcaa tttaaacttt tttgaagggg aagaatattt gtaaaattaa ttttgtgaat
     5881 ttgtggatct taaataattt actaaattct cttgttaaaa aaaattaaat attgtatagt
     5941 aatattagaa tgtttgtaat agaggactga aatgaaaaat ataaatggga aaaaggtaac
     6001 tatttatgat attgcacgtt tatcaggttt ttctccaaaa acagtttcac gagttattaa
     6061 tggtggagta aatgttaaag aagaaactta tcaagcgatt caaaaagtta tagaggaatt
     6121 gtcgtatatt ccaaatgcgt atgcaaaaaa tttaactaaa aaagaagcta taaatatatt
     6181 gatttctgta aaaaaaatcg actcttttcc tttaatttgg tttcatacgc tgttagataa
     6241 agtgttacgg acgtgtaaag aatttggtgt taatgctata gttgagtact ttggagagga
     6301 ggatacaatt agtaattcaa ttatatcaag cacaggtagc ctagtagatg gtgttattgt
     6361 tttttatgaa agtgtagatg atatcagaat tcagtattta aagaagaacc atatgccttt
     6421 tcttgttttc ggtgaatctc aaacgtctgg agtagtctat gtatctaata ataattttca
     6481 agctacttat gatatgatga aagctgtaac tgaagaaaaa tttaaaaata tgtggttgct
     6541 tatgggagga gaatcccatg ttaataagga tcgtgaaaga ggtgtgcgtt cttttctgaa
     6601 tgataaaaat tactttatgg atttaaaagt catttatggc ttatctacaa ttgattcagt
     6661 ttattcttat gcaatgcagc acttgactac ccaaaattat ccggacatta tttttgtttc
     6721 aggagatgaa aaggttcagg gcttgattcg tgcgtgctat gagaaaggaa ttcttattcc
     6781 agatgatatt tctatcattg gatttgataa tattcctatt tctcagtatt atactccagc
     6841 tttgtctaca attgctccca attatgttaa attggctaaa gaaatgatag aaggtgtttt
     6901 agcaattatt aaaggggaaa gtgtcacatc tgttgaagtt tctcctaaat ttgttaggag
     6961 acagagtttc tagtattgtt taattcttga aaatcctagt ggaaaagatg gagtgttcta
     7021 aaataagttc tgtttagcct ccattcagtt tctattgagt tggatccaat ttctaacaag
     7081 tgggacatgt tcatgttcga tctaaggaag atatggtcac attcgaatga tatcaaaaaa
     7141 atcgtaaaaa agtcttgaca aagaaaaaac aaagtattaa aataagttca acaaatcaga
     7201 aaagtaacta tttttgatct tcagggagcc tgtggtgatt gtgaataggt ggttggaagt
     7261 agtgaaagtg ggctgatttt gaaaatgaat ttgaaacaat gaaaattcgg tgcgcacacc
     7321 ttacagtgca gcttgttgtt aaacaaggca gagatgtaaa gggatagtcc ctttataatt
     7381 gaggtggcac cgcgttacca acgccctcac acggaagtat attctgtgtg tgggcttttt
     7441 tctatccgtc gtttggttta tcttttatta gggccttaag tcgctttgat gaacttgagt
     7501 tctatctaca gctccttgcc tcctactaaa agtaaacaaa aaggctggac taatacgaaa
     7561 aaagctagta atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggttgct
     7621 caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagctcaaaa cactgttttg
     7681 aggttgcaga tagaactgac gaagtcagta accataccta cggcaaggcg acgttgacgc
     7741 ggtttgaaga gattttcgaa gagtataaag gtgtaccgag atgaacatct cttcatcaat
     7801 gtctctggtc gtggaggcaa ggacctagct gcgattgcag attactagaa gctaaaaaat
     7861 aatagatgga aaaattacag tcttttctct cacagagagc ttgtggttgc tgaaaacaag
     7921 cagagaaagc tgtaaagttg ggttcatctg aaacgagaga agaaaacatc attctcaagg
     7981 gagtgccctt tatcgcacag cctgttacag gagatatctg agacaggaat gagagatagg
     8041 agaaatccta taattgaggt ggcaccgcga atttcgtcct cacgcaagtt attttgcgtg
     8101 gggatttttc atacagtcgc cacgaactag aattagaact gaaagggaaa tgacaatgga
     8161 acgaatcatt catggagatg tcttatcacc aatcttggct tatatgcgct taaaggggca
     8221 acataaggtt attttagaga gtattccgag agacaaggaa acagctcgtt tttctatcct
     8281 agcctataat ccagtttttg agattaagtt tgaaaatgga gttctctatc aaaatggtca
     8341 agcgattgat cgggatccct tggatttcct ttatgaagtg actcataaga gccagcacca
     8401 ttcagacctc ccttttggtg ggggagccat tggttttgtt ggttacgata tgatttcgct
     8461 ttatgaagaa attggtcaaa tccctgagga tacaattggg acgccagaca tgcatttctt
     8521 tgtctatgag agctatatgg tctttgacca caagaaggag aagatacatg tcatagagga
     8581 tgcactttat agtgagcgca gtcaagaggc cttggaaaaa tccttgaacc aagtgcttga
     8641 ggaattacgc attcctgctc caaatgaatt tgaagacttg gatttatcac cacttgactt
     8701 caaaccgcat atcgctccac ataagtttga gggaatggta gaaacagctc gtgacttgat
     8761 tcgtaacgga gatatgttcc aatgtgtact cagccagcgt ttctcagcag aagttactgg
     8821 aaatccattt gacttctaca gaaatctccg cgtgactaat ccatctaatt acctttattt
     8881 ctatgatttt ggggattatc aaatcatcgg agctagtcca gaaagtttgg tttctgtcaa
     8941 aaatggcatc gtgacaacca atccgattgc agggacgcga ccaagagggg ctacggatga
     9001 agaagacaag gccttggcga ctgacctcct ctctgatgag aaggaaacag cagaacatcg
     9061 aatgttggta gacttggggc gtaacgatat tggccgcatc tctgaaacgg ccagtgtcca
     9121 agttactaag tatatggagg tggagctctt ccgctacgtc atgcatttga ccagtgtggt
     9181 caagggacgt ttgcttgcag aactcactgc catggatgcc ttgaaagcaa cgcttcctgc
     9241 tggaaccgtt tctggagcac caaaaattcg agctatgaga cgcatctatg aactggaaac
     9301 ggaaaaacgg agcgtatatg caggagcaat cggctacttg tctgcgacgg gtgatatgga
     9361 tttggccatt gccatccgaa ccatgattct caaaaatcaa agagcctatg tgcaggctgg
     9421 ggcagggatt gtctacgact ctatcgccca aaacgaatac caagaaacca ttaacaaagc
     9481 aaaatctatg actagaattg gagaactaag accatgattt tattgattga caactatgat
     9541 tcttttacct ataacttggc ccaatacatt gggaattttg cagaagttca ggttttgaga
     9601 aatgatgatt ccaagctgta tgaagaagct gaaaaagcag atggtctggt cttttctcct
     9661 ggtcctggtt ggccagttga tgctggaaag atggaagaca tgatttgtga ttttgcaggc
     9721 aagaagccga ttctagggat ttgtttgggt caccaagcca ttgcagaagt ctttggtggt
     9781 aagctaggtt tggctccaaa agtcatgcat gggaaacaga gcaatatcaa ctttgaagcg
     9841 ccatctgttt tgtatcaagg tattgaggat ggacgtgcgg tcatgcgtta tcacagtatt
     9901 ttgattgagg aaatgccaga agactttgaa gtgacagctc gttcgactga tgaccaagcc
     9961 atcatgggga ttcaacataa aaacctaccg atttatggct tccagtacca tccagagagc
    10021 attggaacgc cagacggctt gtcttctatt cggaatttta tcgagaaggt tgtaaagtga
    10081 ggaaactagg atgaaagaga ttattgaaaa actagcaaaa tttgaaaatt tatcaggtgt
    10141 ggaaatgacg gatgtcattg agcgtatcgt aactgggcgt gtaactgaag cgcagattgc
    10201 ttctctcctc ttagctctta agatgaaggg ggaaacacct gaagaacgta cagccattgc
    10261 ccaagtcatg agaggacatg cccagcatat tccaactgag attcatgatg ctatggacaa
    10321 ctgtggtaca ggtggggaca agtctttcag ttttaatatt tccacaactg cagcctttgt
    10381 cttggctggt ggcggtattc acatggccaa gcatggtaac cgctcgattt cttctaaatc
    10441 tggttccgca gatgtcctcg aagccttggg aatcaatctt gacctcaaac cagctgaact
    10501 aggtaaggtc tttgataaaa ctggaatcgt ctttctcttc gctaaaaata tgcacccagc
    10561 tatgaaatac atcatgccag ctcgtttgga attgggaatt ccaacgatta tgaacttgac
    10621 tggtccactg attcatccaa tggctttaga aacacagctt cttggaatta gtcgtccaga
    10681 actcctagaa agtacagctc aggttttgaa aaatatgggt cgcaaacgtg ccatcgtggt
    10741 tgctggacca gaagggttgg atgaagctgg cttgaacgga acaaccaaga ttgcacttct
    10801 tgaaaatggc gaaatcagct tgtcaagctt tactccagag gatttgggaa tggaagacta
    10861 tgctatggaa gatattcgtg gtgggaatgc tcaggaaaat gcagaaattt tgcttagcgt
    10921 tctgaaaaac gaagcaagtc cattcttgga aacgacagtc ttgaatgctg gtcttggttt
    10981 ctatgctaat ggtaagattg atagcatcaa ggaaggagtt gccttggccc gtcaagtgat
    11041 tgctagaggc aaggcccttg aaaaactcag actgttacag gagtaccaaa aatgagtcag
    11101 gaatttttag cacgaatctt agagcagaag gcgcgtgagg tggagcagat gaagctggag
    11161 caaatccagc ctctgcgcca gacctatcgc ttggcagaat ttttgaagaa tcatcaggac
    11221 tgcttgcagg taatcgctga ggtcaagaaa gctagcccta gtttgggaga tatcaatctc
    11281 gatgtggata ttgtgcaaca ggcccagact tatgaagaaa acggagcagt gatgatttcg
    11341 gtgttgacag atgaggtttt ctttaaaggg catttggatt atctacggga aatttccagt
    11401 caggtagaga ttccgacgct caacaaagac tttatcatag atgaaaagca aatcatccgc
    11461 gctcgcaata caggtgcgac agttatcttg cttattgtgg cagccttgtc cgaagaacgc
    11521 ctcaaggaac tgtatgacta cgcgacagag cttggtctgg aagtcttagt ggagactcac
    11581 aatctagctg aactagaggt agcccacaga cttggtgctg agattatcgg ggtcaacaac
    11641 cgcaacttga ctacctttga ggtcgacttg cagaccagtg tagatttagc cccttacttt
    11701 gaggaaggtc gctattacat ttctgaatct gccattttca cagggcagga tgcggaacga
    11761 ctagccccat actttaacgg aattttggta gggacagctc ttatgcaggc agaaaatgtg
    11821 gcccagagaa tcaaggagtt gcagattgac aaaggttaaa atttgtggac tatcgaccaa
    11881 agaagcggtg gaaacagccg tttcagcagg agccgactat atcggttttg tctttgcacc
    11941 tagtaaaaga caggtgactt tagaagaggc agctgagttg gcaaagctta ttcctgcaga
    12001 tgtgaaaaag gttggagtat ttgtttcacc aagtcgggta gaactgctgg aagcgattga
    12061 caaagttggc ttggacttgg ttcaagttca cggtcaggtg gcagctgatt tatttgagaa
    12121 tttgccttgt gccagtattc aggctgtgca ggtagatgga aatgggcatg tccccaattc
    12181 tcaggcagat tatctactct ttgatgcccc tgtggcagga agtggtcagt cctttgattg
    12241 gggtcaactg gatacgactg gactagcaca gcccttcttt atcgcaggtg gccttaatga
    12301 agataatgta gtaaaagcaa ttcaacattt tactccctat gcagtagatg tatcgagcgg
    12361 agtggagaca gatggacaaa aagatcatga aaagattaga agatttatag agagggtaaa
    12421 gcatggcata tcaggaacca aataaagatg gattttacgg aaaattcggc ggacgttttg
    12481 tcccagaaac attgatgaca gcagttttgg agttggagaa ggcctaccgt gaaagtcagg
    12541 cagacccaag tttccaagag gaattaaacc agcttttgcg ccagtacgta ggacgtgaaa
    12601 ctcctcttta ttacgcaaaa aacttgaccc agcatatcgg cggagccaag atttatctca
    12661 aacgggaaga ccttaaccat acaggagccc acaagattaa caatgccttg ggacaagttt
    12721 ggcttgccaa acgcatgggt aaaaagaaaa ttatcgcaga aactggtgct ggtcagcacg
    12781 gtgtggcaac tgcaactgct gcggccctct ttaacatgga atgtaccatc tacatgggtg
    12841 aggaagatgt caaacgccaa gccctcaatg tcttccgtat ggagcttttg ggagctaagg
    12901 tcgaggccgt gacagatggt tcgcgcgtgc tcaaggatgc ggtcaatgca gcccttcgtt
    12961 catgggtggc taatatagat gatacccact atatccttgg ttctgccttg gggcctcatc
    13021 catttccaga aattgttcgt gacttccaaa gtgtcattgg tcgagaggct aaacaacagt
    13081 accgtgactt gacaggacaa aatctgccag atgccctagt agcctgtgtt ggtggtgggt
    13141 cgaatgccat cggtctcttc catccctttg tagaagatga gtcagtagcc atgtatggag
    13201 ctgaagcggc tggatttggt gtggatacgg agcaccacgc agctaccttg accaagggtc
    13261 gtccaggtgt ccttcacggt tccctcatgg atgtgctcca agatgcccat ggtcaaattc
    13321 ttgaagcctt ctctatctca gcaggtttgg actatcctgg tatcggtcca gagcattctc
    13381 actaccacga tattaaacgt gccagctatg tccctgtgac agacgaagaa gccttggaag
    13441 gattccaact cttgtctcgt gtggaaggga ttatcccagc tttggaatct agccatgcta
    13501 tcgcctttgc ggtgaaattg gccaaagaac ttggaccaga aaagtctatg attgtatgcc
    13561 tatcaggtcg tggggacaag gatgtggttc aagtaaaaga ccgcttggaa gcagatgcag
    13621 caaagaaggg agaagctcat gcctaagaca ctaacaaaaa aattaaatgc tattaaagca
    13681 gctggaaaag gaatttttgt tccctatatc atggctggtg accacgaaaa aggtttggat
    13741 ggtcttgctg aaacaattca ctttttagaa gatttgggtg tctctgcaat tgaagtgggt
    13801 attccctttt cagaccctgt tgcagatgga cctgttatcg aagaagctgg cttgcgcagt
    13861 ttagcccacg ggacctctac ccaggcttta gttgaaacct tgaaaaccat tgaaacagag
    13921 attccactgg tcatcatgac ctacttcaac cccctctttc agtacggtgt ggagaacttt
    13981 gtcaaagatc tggctgatac agctgtgaag ggcttgatta tcccagacct gcctcatgaa
    14041 catgccaact ttgtagaacc atttttggca gatacagata tcgccttgat tcctttagta
    14101 agcttgacca caggaattga gcgccagaaa gagttgattg aaggggcgga gggattcgtc
    14161 tatgccgttg ccatcaatgg ggtaacaggg aaatcaggca attaccgtgc agatttggac
    14221 aagcacttgg cgcaacttca tcaagtggcc gacatcccag tcttgacagg ttttggtgta
    14281 tctagtcaag ccgatctgga acgcttcaat gcggtgtcag atggtgttat cgtcggttcg
    14341 aaaatagtaa aagctctcca ccaaggagag ccgattcagg actttatcaa acaagcagta
    14401 gcttaccaaa aataattaaa caagtagcaa gtaagaggaa aggcacgaaa ggaagtcttt
    14461 cctttttctt ttgcaggcga aaggctagtt ttatagtgaa ttgaatctgg aatattacac
    14521 tacggttcct aaaatatttt aagaaagtga tttgaatttc ccaataaatt tgttcagttt
    14581 ttaattgatt ttactataga agatataatt ttctaaatta ctctgacaca gaactttaag
    14641 aaaaagctaa ttattgtagt aatttcttta agaaaatgat ataattcatt aaaaagtatt
    14701 tggaggagga agaattatgt tgaatcagga tctctttgat tcgcttgagg cacaaaaaat
    14761 tgtagatact ttgatgaaag ggcaaaaaga ttatgtagat gaacgtctag aaaaaagaga
    14821 gacaatgata gtgtcgaatg gttatgcatg gacgtgacct aatcatattg atactgcatt
    14881 tgcatcagca gatttgtttg agtataaatt acaattagca ggacagactt ggggatattt
    14941 agaatttgaa acaaatacag aaaaatatgg gaaagtattg ttaattataa agggtaagaa
    15001 gcgacttacg aaccaatttc ctttggtaca aaaaaataag agtggctact tatttgaata
    15061 tgctcagatg aatacacttt atcttaatca acattcttcc tacaaaaatg atgaagatag
    15121 tcattccttt ccaattcaga tggagttagt ttctgatgaa atgattcaag aaattgaaca
    15181 agctactaaa aattcgaata tcgaaaaatt tatgatttta acttatgagg cggactcaga
    15241 aaacaatatt atatctgtag atgttgttat gcctgatgca cgaactggtc agttacactt
    15301 gattcaagat ttgtctgagt atattcaatc aagttcgtac catttcgaag aagctaaata
    15361 tcaagatatt cctaattttt cagaattatc ggaaacagaa gattttgaaa ttattccaag
    15421 aatagaaaaa caagaaggtc aaaagtagta aggaatatgt aaatgtttaa tggtcgggta
    15481 ttgaaagaat tacggctgtt aaatggttta agtagagcag aattagctca gagaattaat
    15541 ttaacggaac aagccatttg gcagtttgag tccaacgaaa cgaaacctaa attatcaacc
    15601 aaaatgcatt tggccaacca atttcatgtt gatttaactt attttgaaca ggaagaagag
    15661 agcattcgat ttgattcttc tgtaattgcc tttagaaatg cagacctagc aacacggaaa
    15721 acaatagata ttcaaactat gtatttacat aaggtagata gtttgattga ttattttgaa
    15781 agttttgtaa ttatacctaa tattacaatt catgacctaa gtaatgtagt gagtgaatct
    15841 tatcataagg gagaatccat tgaggaattg gctctttatg ccagggaaaa attaggtatt
    15901 tcaaaagata atcatgattt gctttataaa ttagaacgtt caggcatcta tatcgtggaa
    15961 cgattaatta atggccaagc tgatgcttat agcgcatggt caaaattggg aagaccttat
    16021 attgtgttgg gaacgaataa atcatctgta cgtcgaaatt ttgacttagc tcatgagcta
    16081 ggacatattc ttttacacaa atataaagat atgaatgaag atggcaatcg tttggagcaa
    16141 gaagcaaatt attttgcatc atgtttttta ttgccaaaag aagagttttt agtcaaattt
    16201 gaagagaggg ttggcaagcg tgtcagcaat cctgatagtt atattttatt gaagtcggat
    16261 ttgaatgttt cgatacaggc tttagagtat cgagctttta agttaggatt attgactcca
    16321 aagcaacatt cttactttta tcgtcaaatt gcgcaaaaag gttacaaaat gattgaacct
    16381 ttggatgatc aaatttttgt taaaaaacca agcaaagtaa agagtattct ggacgtcgtt
    16441 ttgagtaatc atctagtcag tctagcgact ataatgtcta aacaaagtat tcgtttacag
    16501 tttataagcg aaatattttc agtcgaaatg aaattttttg atcagtatca agaagataga
    16561 agaacagatc gatttgataa catcatccct ttgtacaaaa gaaataattt ataatttaac
    16621 tgtgaaataa tttcatgaag gacatatggc agaaattgga ttttatcaga caagcagtag
    16681 cttaccaaaa ataatcacac aagcagcaag taagaggaaa ggcacgaaag gaagtctttc
    16741 ctttttcttt tgcaggagaa aggccagaat acccgtcgca gaagcgaact gaatcaagat
    16801 cagtaactcc gttacgctaa agacgagagc acaagaagct aaaaagagga aatctcctgc
    16861 gcccatgcgg atatcgataa aatgagccaa aattccaaga gcaaggaagg agaccatgac
    16921 cagattccag ccagaggaag ctattaggat taggtggaaa gtcatccaga ccagtaaggg
    16981 atattcctga tggcgaaagt cgtagatgcc taaggtcaaa ccagcggtga ttaggacgac
    17041 ttgccccaag gaaagcaatt cccaagagta aagcagaaag aggagtccta agactaattc
    17101 aaagagggca taccagacag gatagcgaac tttgcagtag cgacagcgaa agcgattgaa
    17161 gacctgtgag agaatcggaa tcaaatctaa gggacgcaag cgagtctgac aggaatcgca
    17221 gtgactggct gaactgataa tggattgctc tggaaaacgg tcaatgacca aaccaagaaa
    17281 ggaagcgaga atgctcccga cgagaaaaaa ataaaaatca atcatactta tctattcgta
    17341 aaaaatggga gaaatagtat aatggagaaa catagataag atggtgagga aaagatgaca
    17401 attcgttttg aagaaaaggt gagtatagaa aacgctcagc tcgtatgcca atggtccaac
    17461 tccttaggca aagtctttca agaacaatgg atgggaccaa ggattccttt tctactgacc
    17521 cttcaagcct tggaaggagt cttttcaatc tttgatgaac aagagtttgt gggacttatc
    17581 cagaaaatca ggctagaaga cagcaatctt catatcggga gattttttat caacccccag
    17641 aaacaggagc aaggcttagg tagccaggct ttaaggaaat ttgttagttt ggcctttgaa
    17701 aatgaagata tagatagtat ttctctaaat gtcttcgagg caaatcaaag agctcagaat
    17761 ctttaccaaa aagaaggatt tgaaatcgtt caaatggttg aagcacctgt acgaaa
//
LOCUS       DAVBCG010000031        17251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBCG010000031 DAVBCG010000000
VERSION     DAVBCG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.031"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..692)
                     /locus_tag="VQ495_000862"
     CDS             complement(<1..692)
                     /locus_tag="VQ495_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2530840.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDG"
     gene            complement(803..1057)
                     /locus_tag="VQ495_000863"
     CDS             complement(803..1057)
                     /locus_tag="VQ495_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530841.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(1415..1948)
                     /locus_tag="VQ495_000864"
     CDS             complement(1415..1948)
                     /locus_tag="VQ495_000864"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2530842.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVMDLL
                     REVPTIFECLECFFKTR"
     gene            complement(1935..2150)
                     /locus_tag="VQ495_000865"
     CDS             complement(1935..2150)
                     /locus_tag="VQ495_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2530843.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(2117..2260)
                     /locus_tag="VQ495_000866"
     CDS             complement(2117..2260)
                     /locus_tag="VQ495_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET2530844.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            2494..4212
                     /locus_tag="VQ495_000867"
     CDS             2494..4212
                     /locus_tag="VQ495_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2530845.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            4323..4670
                     /locus_tag="VQ495_000868"
     CDS             4323..4670
                     /locus_tag="VQ495_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2530846.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(4949..5785)
                     /locus_tag="VQ495_000869"
     CDS             complement(4949..5785)
                     /locus_tag="VQ495_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2530847.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(5798..6427)
                     /locus_tag="VQ495_000870"
     CDS             complement(5798..6427)
                     /locus_tag="VQ495_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2530848.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(6437..7078)
                     /locus_tag="VQ495_000871"
     CDS             complement(6437..7078)
                     /locus_tag="VQ495_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2530849.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(7224..8453)
                     /locus_tag="VQ495_000872"
     CDS             complement(7224..8453)
                     /locus_tag="VQ495_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2530850.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(8443..9609)
                     /locus_tag="VQ495_000873"
     CDS             complement(8443..9609)
                     /locus_tag="VQ495_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2530851.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            9753..10766
                     /locus_tag="VQ495_000874"
     CDS             9753..10766
                     /locus_tag="VQ495_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2530852.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(10811..11230)
                     /locus_tag="VQ495_000875"
     CDS             complement(10811..11230)
                     /locus_tag="VQ495_000875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2530853.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ495_000876"
     CDS             complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQ495_000876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2530854.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMEF"
     gene            complement(12733..13611)
                     /locus_tag="VQ495_000877"
     CDS             complement(12733..13611)
                     /locus_tag="VQ495_000877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2530855.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(13627..15132)
                     /locus_tag="VQ495_000878"
     CDS             complement(13627..15132)
                     /locus_tag="VQ495_000878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2530856.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQSVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(15147..15683)
                     /locus_tag="VQ495_000879"
     CDS             complement(15147..15683)
                     /locus_tag="VQ495_000879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2530857.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ495_000880"
     CDS             complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQ495_000880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2530858.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ495_000881"
     CDS             complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQ495_000881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2530859.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(16942..17142)
                     /locus_tag="VQ495_000882"
     CDS             complement(16942..17142)
                     /locus_tag="VQ495_000882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2530860.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         5209 a         3781 c         3095 g         5166 t
ORIGIN      
        1 ccgtcagtag tagtcatgct actgagtttt ttatctgtga cattgtagct ggtaccaatc
       61 agttgttttt tattggtaat gtaggttccg tcactttctt tttctccaat tccagtatca
      121 tttttcaatg atagcaaacg cccttgttca tcaacatagc gaactttcac attttctgag
      181 attagttctg ccaattctga ggtttttttc tttttcttga tttcttcggt tattttccct
      241 ttctcttctt cgggaatatt tagttttgga atgatttttt caacaacggt tcgtgatggt
      301 tccacagtat cttggatgac tgaaaagtca gctagaattg ggagattata ctgaacacgg
      361 taactttgag tgtttactcc tactctttca ttattctctg aaaatacttg tacggtataa
      421 gaaacaacat cttttcctaa tagaacatcc ccagtagaga aatagccgcc ttttcctagt
      481 ttgctatctc cagagcccac ttctttccta attttatcag atagtttttt accagtcagt
      541 acattcgttc gcacaatccc tttgtctacc cctacaaagt gggagaactt tttgaactct
      601 tcagaaccag atctagccca accattatta agggcatttg cttttgtatt tgtattctct
      661 ctcaaaggtt tggcgattag aattatattc atcggcactt agagttgctg ctatatctga
      721 ctcttgaata ccaacttcct tactaccatt tctagcggca gtatatgtgt atttatctgt
      781 ttctacttct agctgattat ccttaacatg ataggtaatt ttctcctggt ataagagtct
      841 tcagtagttt atatactctg tcatcatctt tagctttaga agcagctgag tttagccaag
      901 ttggggtatt aggagtatct ttatcaacct gtttcaagac attattcaag tcatttgcta
      961 gcctattggt tatcctcact gtgaaagttt tcttccagtc tgaaccactt ttattagact
     1021 tagatatgct catgaaatcc tggtcaacat tcaacacaat atcgccttgt tttttagcat
     1081 cttccgttgc ttctgcttca aattgatcat ggagtttttg ttgctctggt gtatagtttt
     1141 tttatttaca cgatttaatg tactattttt tattttagta ttaagaactc cattaatacg
     1201 atttttaaac tctgcttcaa aaaatccctt aatttgttgg tgcgcaagtt caacattcaa
     1261 acctttctta tacaagtctt cattctgata caagtcttca ttctgaacga tttcggctac
     1321 acggtgaatg gtttgatcgt ataccgtctt ctcttaacca gcagtatcct tagttttaat
     1381 gactactcga taggttcctt tagaaccgtt atagtcatct agttttaaaa aagcattcca
     1441 aacattcaaa aatagttgga acttccctta ataaatccat cactttcttt ttcaagatag
     1501 cccaagcttg ctcaatagga ttcaaatccg gtgaataagg tggtagaggt aagaaaaagt
     1561 gtttatcttg gatgcagagt tcatccaaaa tgttcttggg atgaaaacta gcattgccca
     1621 tgacaataac atgaggtgtc ttcaaagcag gtaggagttg cgttttgaac cactccacaa
     1681 agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc tataaactct ccgtctactt
     1741 gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct aatcttttca tagactttct
     1801 cccctctagg agcccctgca taaggacgat agaggtagcg gtcgattcct gtttcatcaa
     1861 tatataggac tggtaaatct tttaggttat ccaaaatatc aagaaactca gcgactttct
     1921 ctggctcttg ttccttaaag ctcgtcatct ttttttaaag tgaccttaat ctgctttaaa
     1981 gctgcccata ctgaaggaac agcacaatca aaatgtgccg caatttcccg taaaaaagca
     2041 tctggatgag cctctacaaa ggctttcaat tcctctaaag gaatctttcg gtttttgacg
     2101 actcgctttt tcctctctat gtgtccttgt tcacgtcttt cttttcccac gtgaagagag
     2161 ttctgacgcc aacaccaaaa aactttgctg cctcgacatg gctgtgcccc tctttgatgt
     2221 aatctaatgc tcgctgttta aaatcagtac tatatgccat agctttatta taatatgctt
     2281 gtttaaaact aagcaactat atcaacgttt tgttatgaga agacttactt tttttactga
     2341 aattgaattt ttcctagcct caacatcata taaccccaaa attcaatctg acctggtttg
     2401 aagagatttt cgaagcagat aaggttttac taaaactaaa aaaaatgata taatcaaact
     2461 gattaaatgt tttaaaacta aaggagaatt tgaatgtctt accaagaaaa ttaccagaaa
     2521 tgggttgatt ttgtagagct tcctgactac cttcgtcaag atttggaaaa tatggacgaa
     2581 aaaactaagg aagatgcctt ctatacaaat cttgaatttg gtactgcagg tatgcgtggc
     2641 ttggttggtg ctggtacaaa ccgtatcaac atctacgttg ttcgccaagc tactgaagga
     2701 ttggctcgtt tgattgagtc aaaaggtgga aacgagaaag aacgcggtgt agcaattgcc
     2761 tacgatagcc gtcacttctc acctgagttt gcctttgaat ctgcggcagt tcttgctaaa
     2821 cacggcatca aatcttacgt atttgaaagc cttcgtccaa ctccagaact atcatttgca
     2881 gttcgtcatc tcaactgttt cgcaggtatc atggtcacag ccagccacaa ccctgctcca
     2941 tttaacggtt acaaggttta cggtgaagac ggtggacaaa tgcctccaca cgatgcggac
     3001 gctttgacta cttatatccg tgcaatcgaa aacccatttg cagttgaagt tgctgatgtg
     3061 gaaactgaaa aagcttctgg cttgattgaa gttatcggcg aagctgttga cgtagaatac
     3121 cttaaagagg ttaaggacgt aaacatcaac ccagccttga ttgaagaatt tggtaaagac
     3181 atgaagattg tctacacacc acttcatggt actggtgaaa tgttggctcg tcgtgctctt
     3241 gcccaagcag gatttgactc tgttcaagtt gttgaagcgc aagcaactgc tgacccagac
     3301 ttctcaactg taacatctcc aaacccagaa agccaagcag cctttgccct tgctgaagaa
     3361 cttggtcgtc aagttggtgc agatgttctt gtcgcaactg acccagacgc tgaccgtgtt
     3421 ggtgttgaag ttcttcaaaa agatggtagc tacctcaacc tttcaggtaa ccaaatcggt
     3481 gctatcatgg ctaaatacat cttggaagct cacaaaaacg ctggaactct tcctgaaaat
     3541 gccgctctct gcaaatctat cgtttcaact gacttggtaa cgaagattgc tgaaagctac
     3601 ggcgcaacta tgttcaacgt cttgacaggt ttcaaattta tcgctgaaaa aatccaagaa
     3661 ttcgaagaaa aacacaacca cacttacatg atgggatttg aagaaagctt cggttacttg
     3721 attaaaccat ttgtacgtga taaagatgcc atccaagccg ttcttgtcgt tgctgaactt
     3781 gctgcctact accgttctcg tggtttgaca cttgctgacg gtatcgaaga aatctataaa
     3841 gaatatggct actacgcaga aaaaacaatc tctgttactc tttcaggtgt cgatggtgct
     3901 gaacaaatca aagcgattat ggctaaattc cgcaacaatg ctccaacaga atggaacgca
     3961 acagctatca ctgtcgtaga agacttcaag gcacaaactg ctactgttgc tgacggtact
     4021 gttacaaact tgacaactcc tccaagtgat gtgttgaaat acacacttgc tgacggttca
     4081 tggattgccg ttcgcccttc aggtacagaa ccaaaaatca agttctacat tgcagttgta
     4141 ggggaaacca acgaagaatc acaagctaag attgctaaca tcgaagcaga aatcaatgca
     4201 tttgtaaaat aaagacctat aaaagatgag accaaccaac ctcatctttt ttcgtatcaa
     4261 atctaagcaa ttttagaaac tttatggcat actagactta tcaatgaaag cgaggtaatc
     4321 tcatggaaca atttttagat aacatcaaag accttgaagt cactacagtt gtgcgtgcgc
     4381 aagaagctct tgataaaaaa gaaactgcaa ccttctttat cggtcgcaaa acttgccctt
     4441 actgccgtaa atttgcaggt acattgtcag gtgtcgtagc tgaaaccaaa gctcacattt
     4501 acttcatcaa tagtgaagaa ccaagccaac tcaatgattt gcaagcattc cgctcacgtt
     4561 atggaatccc aactgtacca ggttttattc atattacaga tggacaaatc aacgtccgtt
     4621 gcgactcttc aatgtcagca caagaaatca aagatttcgc aggattgtaa aaaatcagct
     4681 aacaagtctt aaaatcacaa aaacgcataa tgtaaggtgc aaaaaccttg atattatgcg
     4741 ttttattgtt cctaaaacag ttacatcagc atttcattac agaacatttt aattttggaa
     4801 gtcaaatcaa tttttaacaa tgttttagaa atcacagtgg gctattccat attcaattat
     4861 ccagattcaa tcgctattat cgttttccta gattacaaaa aaaactacgt tttgctaatc
     4921 aaaacgtagt ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta
     4981 catcttctcc aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag
     5041 aaaaagcttc atttatcttc ttaaccaagg ttgtatcttc cttacgggct ccaaccgcaa
     5101 aagcttctgt ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt
     5161 ctaaataata gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca
     5221 aatcaatcaa ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat
     5281 tcttcaaaag ttctggattt gcttcaaagt ccgcataacc agatgaacca gcttgagctc
     5341 ctaatgtctt tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca
     5401 atacctgctc attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg
     5461 tagcggaata gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca
     5521 aatcccaatc aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag
     5581 ctaaatcaat atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa
     5641 aagtactatc aaatccaata gtaatagact tgttagactg gtactttgac caattatctc
     5701 cactagtttc gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga
     5761 ctaatacaag catccatttt ttcatccatt tttcctccta tttaggttct actttcaata
     5821 atacatctgc gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc
     5881 caagttccct attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg
     5941 cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag
     6001 ccacccgctg cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta
     6061 gtcctaactg ttccaagagt ccactcgcct tcttctcagc ctcttcctgc ttcattccca
     6121 tggtcttcac aggcgataag gtcaaattgt ccagaactga taaatgaggg aacagttgaa
     6181 aatcttggaa gacaaatccc agtagattgc gcttctgcaa ttcatccagc tctaaaggtt
     6241 gtccattata aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa
     6301 gagttgtctt acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac
     6361 ttagactgaa attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt
     6421 ctaacatggc agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa
     6481 ttgtcacaat cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac
     6541 tagcatcgcg gttagcagct gttcgactag ccaagataag atctgaaatt ccgagagcat
     6601 agaccaaaga agtatccttg accaaactca taacttcatt aaagacacta ggaagaacga
     6661 tcttggtcac ttggggcaag ataatatagc gcactctgtc aaaagggcta aacttcaaga
     6721 ccttggcggc ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg
     6781 caaagtaagc tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac
     6841 gaatcccaat acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc
     6901 ctcgcataac ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca
     6961 aggcaaaggc aatcaaaacg cccaacggaa tcgaaaatag caagaccagt gcaaagacct
     7021 gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac
     7081 tgcacctcct aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt
     7141 tttgtaaaaa gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta
     7201 cagttctata tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca
     7261 tctggaacca gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat
     7321 tcacgcaaga gatagatata gtgttccaga aattctggat tgtccttcaa atctcctgtc
     7381 aactcttgat aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca
     7441 atcatccact tggtcaaaag attttctggc tcctcactct gcaagtctag aatatcctca
     7501 taacgctcct gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca
     7561 gcgtcttctt ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa
     7621 gaaaattgtg aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc
     7681 ttagcgatac gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca
     7741 tactcatagc cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct
     7801 ttttgatatt cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg
     7861 tattctaact ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga
     7921 gcataggcaa agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga
     7981 ttatctaact gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac
     8041 tctgccaaac ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc
     8101 tcacgtgcca catctgtcaa accttccagc tggtaaaggt ctgccttcag agccaaagac
     8161 gagacatacc agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt
     8221 tgaccatcct cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca
     8281 attttcaggt aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc
     8341 aactcataca gaagatcact tgaatcattt tctaaagctt tggcgaaata atgctcagcc
     8401 ttagttaaat cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac
     8461 ttcctcaccc tctaattcat atagaccact gactacctta taccattcga aaatggctga
     8521 aatgacaacc ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa
     8581 catagatcct gaagttaaca aaacaaagag aacattaata ggatggatgt ttaattgact
     8641 tcccaaaatc aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat
     8701 cactactttc aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat
     8761 ggctagaaag ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt
     8821 aaccgcatag cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat
     8881 agccactgtc acttgccctc gaacatagtt ggacaactgt tgattcacat ctgataaaac
     8941 ttgtccaaca ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc
     9001 tttcccatca cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac
     9061 ttgagaagcc ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact
     9121 tgccaaaact gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa
     9181 atcatctggc aggtgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta
     9241 aacaggaacg tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc
     9301 caagccccaa atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg
     9361 attaacctta tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc
     9421 agacaaaatg actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa
     9481 actaatctta cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt
     9541 aattgccttg ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt
     9601 ttgctccata aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata
     9661 actctttttt aaagtgctta catctaattg gatgtcttta atttgcttgt ttttgtgata
     9721 taatagtctt atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt
     9781 atacacgtcg tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc
     9841 tttcaaatct agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc
     9901 accaacattt atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa
     9961 ctgacgggac tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg
    10021 tcgatgaaaa gcgtgatttg gtttacgcag ccaactatca caagggacaa gtccttgttt
    10081 ataaacgcca ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag
    10141 gtccacatga aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact
    10201 atctagtgac ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag
    10261 gaaaattatc taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca
    10321 ttttccataa ccactataaa atcgcttatc tcatttgtga actcaatagt actatcgaag
    10381 ttttaatcta cgatggcgtt ggcgaatttg tacgtatgca ggttatttca actttaccag
    10441 aagcttacga aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc
    10501 tctacgcttc taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta
    10561 gcttagagtt attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga
    10621 cccccgacca aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta
    10681 aacgtaattg tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag
    10741 cagtctgcat ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt
    10801 tcattttttc ttatagtcta tttccgacat taatacggtt aatggcacgt tgcaaagcaa
    10861 tcttagcacg acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt
    10921 ctgcacgaag tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag
    10981 agtcagcgac gattgtgatc atatcattgg caatttcaat aacgccaccg tttactgcaa
    11041 tccagttcac gtgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa
    11101 ccgcaatcat attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg
    11161 atacatagct ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta
    11221 actgagccat aggtcacctc ttaaaattcc attttttcag cttttgcaat cacatcttcg
    11281 atagaaccta caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt
    11341 tccttaaagc cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat
    11401 tgttccgcaa cgttgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc
    11461 aaggtctttt cttcatcaga aagttcatcc ataccaagga tagcaatgat atcttgcaat
    11521 tcatggtaac gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca
    11581 acgatttcag gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata
    11641 cccaattgta ccaacttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt
    11701 gctggcgctg ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca
    11761 gaacccttct tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt
    11821 ggttggtaac caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc
    11881 tgagtgaaac ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg
    11941 aagtattcag cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca
    12001 ttcatctgac caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag
    12061 taaaggtcat tcccctcacg agtacgttcc ccaacaccag taaatactga aataccaccg
    12121 tgctcttggg caatgttgtg aatcaattct tggattaaga cggttttacc aactccggca
    12181 ccaccgaaaa gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg
    12241 atccctgttt caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta
    12301 tgaattggct gacgctctgc gtcttctgta aaaggagctt ccaaatcaat ggtatctccc
    12361 aaaacgttga agacacgtcc caaagtttct ttacctactg gtacagagat tggacgaccc
    12421 gtgtccaata cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga
    12481 accataccat ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca
    12541 tttttgtaga cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa
    12601 acgtctacaa cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat
    12661 tctatataag atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag
    12721 ctggactaga gcctattcta aggcactagc acctgctacg atttctgtaa tttcttgtgt
    12781 aatcgccgcc tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc
    12841 attatctgtc gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc
    12901 gataatggca ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc
    12961 ttctcggctg gtttccaatt caaaagtcaa gctgtactct tcatccgctt catttggatc
    13021 caagtcaaca atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg
    13081 gtggttgtag caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt
    13141 tgaaataatc ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata
    13201 aagtggttga ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat
    13261 ttcaaaacct ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat
    13321 agaggaatta taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt
    13381 cttcacagaa cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag
    13441 gatatctgtc aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc
    13501 ttcagagcga cctaacttag cagccgatac catttgcatg gcattagtga tttgactcgt
    13561 attttttgtt gaggcgattt ttgttttaat atcatttaga gacactgcca tctgacacct
    13621 ctattcttat tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc
    13681 ttcttctggc aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc
    13741 aaagaaggca tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa
    13801 gaaaccatgt gtcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg
    13861 aacaggttgt ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc
    13921 tgcgtccaag tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc
    13981 gatacgaagt gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg
    14041 agatacagat gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc
    14101 aaggaagatt tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc
    14161 tcctgcttgt gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga
    14221 aactttagct gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc
    14281 ttcacgacct ggaggacgac gaagcaagag cgatagttca cgataagcta ccgattgttt
    14341 tgatagatca tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat
    14401 agcaacccca gtataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt
    14461 cacaacgatt gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac
    14521 tgttgattct ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt
    14581 caagattgta tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc
    14641 acgttgacca cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg
    14701 ttctgaaaca gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt
    14761 atcagtgtgg atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc
    14821 aatcagactt tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc
    14881 ttcacggata tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt
    14941 ttgagccata ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc
    15001 aaggccatga gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac
    15061 atcaaaattg ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc
    15121 gttaattgcc aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt
    15181 tgtttaatac tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga
    15241 ctttcatcga tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa
    15301 gggagcaaac gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca
    15361 aatcgatttg tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga
    15421 tttgctctgt gattgtaggc cagaacctgg ataaagtttt gtaataaagg cgacacagaa
    15481 tcttggaaaa aagcaattgt tttttcctta tccgactcgt ctactgccac ttgttttaaa
    15541 aaagaaggca gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata
    15601 cggtcttctt ctcctttttc aagtaccaat tggacaaaag gcatgctgta tttttcaatt
    15661 acctttactg ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga
    15721 gtgctttatg ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg
    15781 tcaaatctgc gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt
    15841 cttgattggc tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag
    15901 ccttactttg ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc
    15961 taccagccaa ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt
    16021 cagctctgtc aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat
    16081 tagaccatgc aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa
    16141 taaaattacc aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct
    16201 tcaatattaa tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg
    16261 atgcaggaaa taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga
    16321 taccaataaa tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca
    16381 aagatattcc caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata
    16441 ttcatcggta tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga
    16501 cgacgaacgc tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac
    16561 tgtaaattag cggttggcga actccaccag ttagtcccat cgatcgtttg aagctttgtc
    16621 attaagccaa ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgag
    16681 taatctttca tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag
    16741 acatactcaa gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa
    16801 taaataaaga caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg
    16861 aagataacag gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc
    16921 ttttcttctc gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa
    16981 agttccttca ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc
    17041 tggttggcgt gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac
    17101 gcccatacag gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa
    17161 ataaaattta cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat
    17221 tggaagcgtt taataaaaat tatttccatt t
//
LOCUS       DAVBCG010000032        16863 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBCG010000032 DAVBCG010000000
VERSION     DAVBCG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.032"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(87..1067)
                     /locus_tag="VQ495_000883"
     CDS             complement(87..1067)
                     /locus_tag="VQ495_000883"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2530861.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(1069..3321)
                     /gene="pbpX"
                     /locus_tag="VQ495_000884"
     CDS             complement(1069..3321)
                     /gene="pbpX"
                     /locus_tag="VQ495_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2530862.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRLRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKATAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(3325..3642)
                     /gene="ftsL"
                     /locus_tag="VQ495_000885"
     CDS             complement(3325..3642)
                     /gene="ftsL"
                     /locus_tag="VQ495_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2530863.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(3654..4604)
                     /gene="rsmH"
                     /locus_tag="VQ495_000886"
     CDS             complement(3654..4604)
                     /gene="rsmH"
                     /locus_tag="VQ495_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2530864.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIHQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            4770..4964
                     /locus_tag="VQ495_000887"
     CDS             4770..4964
                     /locus_tag="VQ495_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2530865.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            4980..5522
                     /locus_tag="VQ495_000888"
     CDS             4980..5522
                     /locus_tag="VQ495_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2530866.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSVLGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            5533..5775
                     /locus_tag="VQ495_000889"
     CDS             5533..5775
                     /locus_tag="VQ495_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530867.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            5786..6121
                     /locus_tag="VQ495_000890"
     CDS             5786..6121
                     /locus_tag="VQ495_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530868.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTTPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(6231..7232)
                     /locus_tag="VQ495_000891"
     CDS             complement(6231..7232)
                     /locus_tag="VQ495_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2530869.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKKLNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7292..9193)
                     /locus_tag="VQ495_000892"
     CDS             complement(7292..9193)
                     /locus_tag="VQ495_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2530870.1"
                     /translation="MKEERRQFFERIDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(9215..9508)
                     /locus_tag="VQ495_000893"
     CDS             complement(9215..9508)
                     /locus_tag="VQ495_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2530871.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(9508..10326)
                     /locus_tag="VQ495_000894"
     CDS             complement(9508..10326)
                     /locus_tag="VQ495_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2530872.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(10313..11092)
                     /locus_tag="VQ495_000895"
     CDS             complement(10313..11092)
                     /locus_tag="VQ495_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2530873.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(11107..11598)
                     /locus_tag="VQ495_000896"
     CDS             complement(11107..11598)
                     /locus_tag="VQ495_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2530874.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(11609..12799)
                     /locus_tag="VQ495_000897"
     CDS             complement(11609..12799)
                     /locus_tag="VQ495_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2530875.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(12811..13245)
                     /locus_tag="VQ495_000898"
     CDS             complement(12811..13245)
                     /locus_tag="VQ495_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2530876.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            13516..14331
                     /locus_tag="VQ495_000899"
     CDS             13516..14331
                     /locus_tag="VQ495_000899"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2530877.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            14350..14991
                     /locus_tag="VQ495_000900"
     CDS             14350..14991
                     /locus_tag="VQ495_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2530878.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            15022..16023
                     /locus_tag="VQ495_000901"
     CDS             15022..16023
                     /locus_tag="VQ495_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2530879.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            16033..16662
                     /locus_tag="VQ495_000902"
     CDS             16033..16662
                     /locus_tag="VQ495_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2530880.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
BASE COUNT         4994 a         3778 c         3068 g         5023 t
ORIGIN      
        1 agcctaaagt tagctttcta gtttgttctt tgattttcat tgtgtattta aatacaaaaa
       61 caccctgaaa catcaggatg ccattcttac attaaataca aaattgctag ggtcagaaga
      121 cttgctagaa gtcccactcc ccaaaagaag aagtcaacct tccactcgct ccaaggattt
      181 cctttaccag acaatccccc aagctcaaaa tggtgatgta caggcgtcat acggaaaata
      241 cgtttaccac ctgtcagttt gaaataactg acttgcatca taacagaagt tgtttcaaaa
      301 acatatacaa ttccgataat caagagagtc cattcttggt ggagagccat agagatagct
      361 gccagcatcc cacctagggc caaacttccc acatcaccca taaagacctt ggcaggctta
      421 tggttaaaga tgaagaaacc gagcaaacca ccaatcatgg caagaatcac tagaagaata
      481 tccatctgac cttgcacata ggcaataact ccataggcag acaaactaat cacaacggaa
      541 atactagcta aaccgtcaac accgtctgtc aagtttactg cgtttgaaaa accgactagc
      601 cagaaaagag cgaagaaaat atagaaaaat cccaaatgaa ctggataacc aaagacagac
      661 aggatatcgc ccccacgctc atagaaaaga tagaagataa ttccacctag aagctgaagc
      721 gctaattttt gcttaggatt aagcccctca ttgattttac gaaagacctt gagaaagtca
      781 tctaaaaatc cgaccaagcc atacaagact aatatgaaca aaatcattcc cacattattg
      841 ctgaattggc tactaaatag ggcgaaaaag aaagcaacca aaacagaagt aatcaagaaa
      901 accaaacctc ccattgtagg agtcccagct tttgcctgat gctgtttgac atcttcatgc
      961 atctgctggc ctgtaatttg cgcctttcta taaaattgga taaaggccgg aattcctact
     1021 aaagttagta aaaatgtcac aattccagca ctgatggaaa taaacatatt agtctcctaa
     1081 agttaatgta atttttttaa tgtccttgat agctgtgtta gcacgaacat cttgcttctg
     1141 cacagtagag cccgaacctt gaaattcaag ttctatattg agccacttag caaaggtctc
     1201 agcagtcgct tttgtccaac catacatatc tggaacttcc tctgctttat cagataagat
     1261 aaggacttgc tggttcgggg caagattctt cccttcttca gcagaactgt ttttaatctt
     1321 cgttcctgtt cccacaacga tgggttgtac aagattgcga cgcaattctt ctgctaaatc
     1381 accaggtgaa atatccttga cactaggcat aggataagga ctttgttgac ttacttgctc
     1441 caaagcttta gctgttgttt gaagattgag agagtctttc atagctgaag cccgctccaa
     1501 gataggattg gcaaattctc ccaactgaat acctgaataa tgttcaggtt gttggaccgt
     1561 cacatacaag ataaaatcag gattttcagc cggactcatc gatacagccg agaaaatata
     1621 gtcggttaac ccgactagat aaccaccatt tttctcgtca gcaatctgag ccgtaccaga
     1681 cttgagggct acattttgcc caggaacagt tacagttggc ttgcctgtgc tgtggttata
     1741 catggttcca taaaccggat ccgtccctac caaaaccatg ttagtccgag ttagactagc
     1801 tgcatcttta gaaacaggat ttcccacaat ttctttttga gatttccgag cagtttgatc
     1861 atttggatca taaatggcac taataaattt aggctccagc atgacaccgt cattagcaat
     1921 agctgtaaag gcacgaatca tttgtgtctg ggtcactgaa atcccttgtc caaatgaact
     1981 ttgcgcaatg ttgacaatat tatccgcagg aagctgacca gcatactcat ccgtcaaacc
     2041 gaaacgggtc ggaactccaa atttaaaacg attaagataa tcaagccagg tagcatctcc
     2101 catcttttgc tcaaggaggg tcatcccaac gttacttgag tgtgcaaaac cttgagaaaa
     2161 agtcatcatt ctgccaccag tcaatccttc attaacgtcc caatctcgaa tcgtggcatc
     2221 tgcaattttt aactcactac tattaaagac ttctcctcct ggaaaggtat tattatcaat
     2281 agcagcagcc aacatcatca ctttcatagt ggaacctggc tcatagttac tttggtaaag
     2341 gatatcacgc caaacaaagt cctctgtaat gccttctttt gtatctgcat caaaggtcgg
     2401 tcgttgcgtt gttgccagaa tttcccctgt tttagcactg accaaagtcg ctgtcatgta
     2461 ctttcctttt accttctctt gaaaagcatc catctgggtt tccataaagg actggagagg
     2521 gctggaaatg gttgtataaa catccttacc gtccatcgtt cgttgggaaa cttgttctgt
     2581 tccgggtaca atattaccca gacgatcctt ttcataggta ataatgccgt ctgtccctgc
     2641 aagaatactg ttcaaggaac tctccattcc agaggttccc agcaagctct tgcttccatc
     2701 ttcattttca tggagctgag ctaggccgat aaaactagaa gcaaattgtc cgtttgggta
     2761 actacgattg ggactggttg taaaatcaat ccccttgacc tctgcagctt ccaattcttt
     2821 tttgatagac atcatattgg cataggtaat cccatttccc tttgctccaa aggaaacttg
     2881 cttgagatta ggttgcgaga gttgctctct tacataggat tcttccatgt ccagatactt
     2941 atgaaagacc tctgcaacct tgttaaattg tgttttttct acgtaaagaa tcttacccgt
     3001 tgctgactta tagttctcat caatgaccgc atagacatta taggaggttg catcctcagc
     3061 aatcgggact ccatttcggt cataaatagt cccacgtttg gcaggaactg tacgggtggt
     3121 ttgatgaacc ttcttagctt ccttcgctaa atctgttcca aagcgagtgc ctgtcccaat
     3181 aatgaccgca aaattgacta aaaaaatggc aaaaacaaag acagataata aactcagact
     3241 ttttccaact ctgagtctgt tttcagccgg cgatttccga tttttggtcg cataacggat
     3301 tactcttttt gtccacttca tatcttactc cgctattcta atattttcat tgtttaattg
     3361 caaatcgtgt gaattggcaa tttctttcaa acgttctgca cgtaatagtt cattgacctc
     3421 ttgcttggca tcgtccaatt cggttttctt ttcctctatc tgcgcattga tttttgtcaa
     3481 atcattctgc acttgcaaga gcttggtctg cataaaaata atactaatgg ctacaataag
     3541 agtggttaca gcaatggaaa agtaaaaagc tttttccaca cgcgaaaacc gtttaagttg
     3601 catctgtagt atttgacctg ttttttccat tttttctgcc atctttttcc ctcttacttg
     3661 tgaatttttc tggccacgcg caacttggct gagtgcgagc ggttattggc ttctaactct
     3721 tccgcacttg gcaagattgg cttacgggac accaattcca tcttgggctt gagatcatct
     3781 gggatgaaag gcaagccttt tggaacttca actgttgaag cttccttgaa caattgcttg
     3841 gtcaagcggt cttctaagga atgaaaggta atcactgaaa ttctaccatc cagagccaac
     3901 atatccatag cctggtggat ggactcatct gccgccccca gttcatcatt gacttcaatt
     3961 cgaatagcct ggaaaatctg cttagcagga tgccccttct tcttgagttc cttggcaggt
     4021 ttgaccaact tgataatctc tgctaactca gtcgttgtct caatcggctt cacttcacgc
     4081 gcttgctcaa tcttacgcgc aatctgttta gagaatttgt cctctccata cttgaagaaa
     4141 atacgaacca agtcatgata gtcataattg ttcaccactt catacgctgt cagactagca
     4201 tcctgattca tccgcatgtc cagtggcgca tcctttttat aagaaaaacc acgctcacgc
     4261 tggtctaatt gaggactaga cactcccaag tcataacaaa ttccatcaat ttcctgaaca
     4321 ccagcttcgc gcaaacgtgc ctgtaaatga cggaagttat ccttgataaa ggtcaccatt
     4381 cccttctcaa tgtaaggtgc caagcgtttt tgcgcattat caatggcatt ctgatcctgg
     4441 tcaaaggcat agagatggcc tttttcactt aatttactta ataaatactc gctatgtcct
     4501 gctccgccca aagtcgcatc aacgtagata ccgtcaggct ttacgtcaag catatcaatc
     4561 gtttcgtgga gtaagaccgt tacatgatga aattcttttg tcatatctta tctattttac
     4621 cacaaatccg accagcttgc acttgtcaga caaataggac aaggagacta ctatttcttc
     4681 aaaaaatatg tcaaaaatac ttgacacttt atgtttaaaa gactataata tagtcaaata
     4741 tatacgacat aaatcagaaa ggagaacaga tgaatcgtgt gaaagaattt cgcaaggaat
     4801 tgggtatttc ccagctcgaa ctcgccaagg atatcggtgt ctcgagacag actatcaata
     4861 tgattgaaaa cgacaagtac aatccaacct tggaactctg tctcaatctc gcccgcagcc
     4921 tccaaactga cctcaacagt ctcttttggg aggaagattt ttaaaaaagg agcaaactta
     4981 tgaaaaaaga aaccttcact gaaaaactga tcaaacgcac atacggtatt tctggtcccc
     5041 ttgacgaata caaacggcgt gaggccgata gtattgggaa ccaagttttt atcatcctct
     5101 tttatctgat gattttcgga aatcttattc cactccttct ggcctataaa taccctcaag
     5161 aagtggctct aatctatcct cctctgattt tagtgattgc cctcatcgct gctggctatg
     5221 tcacctacca aatgaaaaaa acaggcatca cagtcattga accagatatg ctgaatgaga
     5281 aagaaagcaa gcaactacac tacccaggtc tgaaagcagg tttgttcttt ggtctatgga
     5341 tgttttttat aactcctctt ctcagtatac tcatagatga aggtcaggac tattttcatt
     5401 ctcttctcac tataagaaat ggtgtatcaa gtgttctcgg ttctattttc ttcggagcga
     5461 gcatacagtt cctcatctcc cgtcgcattg caaaaactaa gaaaaatcaa gatgaggatt
     5521 aggaggaaac tcatgaaaaa agaaaagaaa cagttacgtt atcctggtct gaaagcaggt
     5581 tcgatttatg gaacagtgat attttttata attccactca ttgataccct aacaagtgaa
     5641 aatccaaatt ttatcagttc tcttctaaat acaaaacata tttttaaaac tatactggga
     5701 gctttctttt tcggagtgat gatacatatt gtcgactcac ttcgtattgc aagagctaaa
     5761 aaagaccagg attaggaggt gccttatgaa atcactagct agactactga tcattcatgt
     5821 ttttatcagt attttccttt tcttcgccct tatttcagga gctgtttctc atacagtttt
     5881 actactccta ctcctctttc ttcctgcgct caataaagga cttgagaaaa tacaatcaaa
     5941 acggatacct gtcctcaacg cagccctctt ctttctcctc atatcctttc cacaactttt
     6001 aaccacccct gtccaatgga aattttcaat attcctagtc gtaaccatca tttcaagttt
     6061 ggcctacttc tataactttt atcaagtagt taaagaagta gatcaaaaac agttgattta
     6121 ggagcttgct cccataattt ctcatttcat aggcaaataa tcccacctag cttgttctag
     6181 gtgggattat tttatttctt aacagagatt gcaagtcatt ttccttcatt ttagaaagtc
     6241 gactctttcc aattcacact acaatccaag acttgttgcc tttcttcttg attgcggcct
     6301 tcaatctggt caatcaaaat ctttgtagcc tgttgtcctt cctcaaagga gggctgaacc
     6361 agcgtcgaaa cacttggaga agaaaagcaa gtccactccg tattgtcaaa accaatcaac
     6421 ccaacttgtg gcaagttata attcaacttt ttgataacgg taaagactag aggtagggcc
     6481 caacagttag ggataaatac cagagttttt tcatcgggat cgatttcttt ttgtaaaaat
     6541 tccttaattt gttccaaatt cgtatgctta tcttcaatgg ttagactggc gtgacgcata
     6601 ttagcatctg ttaaagcatc cacaaaacca cttgcccgct caatccgagt actcaaacga
     6661 ctcgtatccg ctgtaatcaa gagaaaatgt tcataacctt tttcgataca ggactgggtc
     6721 atgtcataaa cggcatcata gttattggtt ttaacccagc tagtccggtg ttcatagagc
     6781 tgactatcaa aaaagaccat tttcttcttt ttctcatcga tgatacgaga atattttcgg
     6841 aaattagagg tcggctgaat aataaagccg tctactccca agagaagcat gctttcaata
     6901 taccggtcct cactctcttg gctgtaatta ctatttccta tcattacctg gtagccattc
     6961 tggctggcga tatcctcaat tcccttaaca atttggtttg agaaactgtt ggtaatatca
     7021 ccgatcaaaa caccgattaa ttttgttcgt ttggagttta agctacgcgc aacaatgctc
     7081 ggtttgtaat ttgtttcatg aataactttt tcaatctttt cacgtgtctc ttgggacatt
     7141 ttttcatatt tcccgtttag gtaaaatgac acggttgttt tcgaggtctg agccatttcc
     7201 gcaatgtctt ttatggtcag tttcttctcc aatctaaaac acctcctact aatacttatt
     7261 ataacatgtt cactattcaa aatgcctatt tctagcactg caagcgaatc attctctcat
     7321 ttattttatc atatactaga cattttccac gcatttttgt tccgtcaacg aggcacaatt
     7381 tttcacctac accaatatcc tcgcttaata acagaatcag cttgtctgtt tcgtcatttt
     7441 tcacttcaaa agccagagca ttttccactt cacgatcatc tgtctggtag acagaatgac
     7501 ggatgatttc aaaactctca tgcgcaatga tggtataatc cagcatctga ttctcaaaga
     7561 attggcgctt ggtgagtttg ctacttcttt caagctcatt gtatttagga gaaatgatgg
     7621 tatcttccaa atcaaaatca acttcactcc atagtctcaa ctgattgatt ttcccatctt
     7681 gataggtcac atccttgtca aggataaact gagtcaacgc ctcatgctga ccttgacacc
     7741 tgatgtcatc caccaagagc cagacatcct ctaccaacat gaggattttt cttctgtgaa
     7801 gataaggcaa atcaggttct gctgaccaat aagccccctc aatataatgc actccctccc
     7861 tttctttatg gtgacaaaac agggagtgag gatagtattc atattcccag gatcctgtga
     7921 ttctttccgg agctttccca tctacaatgc aggtcgaatg actccaagca ctctttaaga
     7981 gataacgttc atatatctcc cgataagaat aacgcccagc atctatgaaa ataggttggc
     8041 cttgatactg taagcaaaaa ctattctcgt cactatggct atgggcactt cctagcggac
     8101 catttttgaa aaatagataa cgatgttcat ccttaataca gacatgtcca gagtcttcaa
     8161 agatcatgga cttaggctgc caagctctcc tttcaaattc ctgcagtcgc ttgacctttt
     8221 ctcgccccag gaacaagagg ctaagcaaat caactttaac atccagaccg ttaagaaggt
     8281 cttccttgtt caaaaccaca gcagacaggc tcaaaatttc tgtcgtttct gtagaatcgc
     8341 tatcaccaaa agccaaagtt cgtccatcta agcctgtcat catttgaata taggtcgcca
     8401 tcttttccag caactcttgg taactatctt gcaagtctgg aagcaagaga cacaaatcca
     8461 gcaaggcttt ataaacctct acatgataga gaatcgactg ttcaaactgg cttccatctc
     8521 ctaaaatctg tgtctcaatt tgctgtttca actcctctga agcaaaatgg taagcttctt
     8581 ctaggtccat cttatctgaa aagaaatgat agatagcaag catcggaatt gtttgtaaaa
     8641 tcccccagtt actaagggtg tacttggcgc gatagtagct tttcataaag tcaatctgct
     8701 tttctagact gaccaaaatt ttctctagtt ctttctcctc tagcaagtca aatttcaaga
     8761 gaagcaagag tagtttcaac caagtaaagg aacgaatacc cgtatccaag gttctagtca
     8821 tcaaggattg aggagaaaat tctctcacct gctcaatcca atcaaataga aagaacttgc
     8881 acttttgaat atagtcctta tctccttcta ccagataccc tatcataaac tgcaagagat
     8941 attcttgtcg attgagcata taagaccatt ctggatcatc ttcaaatact tgatcccata
     9001 ccatcggctg gatttgatgg atttttgaac aaggctccat atcccaagga ctatcaaaca
     9061 taaaacgatt gtccatcaag cgttcaaggg aactcttgac tttctcatag tcttttgaac
     9121 agtgggacaa gatataatca cgacattggt ttccatcgat tctttcaaaa aattgtcttc
     9181 tttcttcttt cattatctat taccagaaaa agaactactt aaaaagcagt tcttttgtct
     9241 ttcccattac actttccttt tctacataga tgaccacacc ttttgcaatc tgcaaggaga
     9301 ccaagtcatc tttgatagaa atgatttttc catgaattcc agacaataac aacacttcat
     9361 caccagatgt taaagaagct aaatactctt gtcgttgctc catctgtttg cgaagcaact
     9421 tttgctgacg aatagaatga aagcttgaca gtaaaagggg actcactgcc aagacaatca
     9481 ctattccata aaacaatgtt gtatccatta agctataatc ttaagccagc ttccgataat
     9541 tccgatgata actgttaaaa taacgagttt atatgttgtc catttctttt ctttgatcaa
     9601 gtagtaaact aaaagtgtaa atagggctgg tagaagagct ggagcaacct tatcaagcat
     9661 tccctgaata cttacgatac tttgtttagc gtctgcttta acttcccctg cagcaaaggt
     9721 aatcggcacc ataatcttaa cagatgtcgc tgccaaacca gcaattacgg ttacaccgat
     9781 aatattggca atacgagaaa tcgttgccat ctgttcgctt agtttatcaa tcacagttgt
     9841 tcctagtttg tatccataca gaccagttga caatttaatc gctgttaaaa tcgtattcat
     9901 cgcaaggaag aacaagattg gaccgacaac caagccttct tgagcaaacg aagctgcgat
     9961 ggttgagaac aatggagcta aacagaattg agaaagagaa tccccaatac ctgccaatgg
    10021 tcccatcaag gccatcttga tgctacgtgt ttcttttgtt ggtcggccat tttccaacat
    10081 tacaagatgc aagctggtaa taaaaggcag gaagtgtggg ttggtattat agaattcaca
    10141 gttttcttcc aaggcttggt agaaaccttc ctgatcctct ccatagtgtt ttttcaaagc
    10201 aggatacatc acattggcat atcccaaccc ttgatagtta ctatagttaa atccattttg
    10261 acaaaagaat gcccgcaaag acgttttaag ataatcacgt tttgttaatt tgttagatcc
    10321 agtcatcgtg tgcttcctcc tctaccacat gatccgctgt ttttggcttg ttataaaatt
    10381 caatcaaagc aaagatagta cctacaattg caataccaat tgttgggatg tttagataag
    10441 ctgcacaaac atatcccaac aagacaaagg gaatcaactc tttcttagcc atcactgaca
    10501 agatcatcgc aaaaccgata gctgggagca ttttaccagc aactgtcaaa cctgtaagca
    10561 atactggtgg aatgtagtct acgagtttca acaaggtatc cattgaaagg gcacccaagc
    10621 aacccaaggt aaatccaata aaggcaaaca accaaattgt tgcatttaga gtgaacttga
    10681 atttcttcaa attatggttt ttcaagtgct tcatcgccgt ttcaggcgca ccagcacgta
    10741 cagtgtaggc gaaagtttgt aagaattgaa tcgccacagc aatcggagta gagagggcaa
    10801 gagccgcttc tggactgact ttaccagcac tagtgatagc catcaaggta ccaaagatac
    10861 caggtccgat tgggtttggt ggaacagtac ctccagcacc aacaccgaat cccatataag
    10921 ccaattcacc aatagctccc attgcaagag cagtaggcaa atcacctaga ataattccga
    10981 caccaaatga cagaacaata catctattgg tgtaaattcc taacagcatt ccactaaaac
    11041 agaaagctgt ccataatcca attaaaatcg cttgaaatac attaatcgac ataacgatct
    11101 cctcttttaa atatagtcca taatgttgac ttgaacagct ccatcatttc ctgttggagt
    11161 tgttttcgta ttaaatgtta cttgatgagt ttggctcaat tccttgaggg ccgccttgtc
    11221 ctcttcaccc aggaagatgg agcgtgtcac ttgctcttta ccagtagcat tgtgaatgtt
    11281 cccaatattg atttctttga tagggacacc accttctacc aaggttaaag cgtccttcac
    11341 atcctttaca acgataaaga tcgtttgagc aggattagcc ttgtgaatga tatcaatcac
    11401 cttttgcaaa gggaagaaac gcatggcaac tgagtctggc acaactgttt tcatcagagt
    11461 ttgctgcatc ttgtctgtgc ttacttcgtc attggcaaca atgaccgtat tacaacctag
    11521 gtattttacc caaagttgtc cttgcccatg aatcaaccgt tcatcgatac gggtcataat
    11581 aatatttggc attgtcatat ttctcctcct accaatatag gttccagtct ttgtagaaac
    11641 ggataagggc ttctaggtaa tagtagtcac cccagatatt gccttcatcc actcctttac
    11701 ctgaatgcca tgagtacaca ccgtggagga gacttgtccc accaggggta aattgatcat
    11761 ttgcataatg ttcgatcaag gaacgaagca tggcatgcat agcatgttta taaatatctt
    11821 tgtcagcatc cacctctggg agatgtttta gcatttcatg aatcccacag acggcgatag
    11881 ctgttgctga agaatctcgt gattgagcac taccatcatt aaaaatcaaa tcccaatagg
    11941 acacatgatc ttttggcaga cgattcaaga aataattggt cacaccctta aacaagtcaa
    12001 agcaggactc gtcttttaag tgacgataag tcaaaggaat accatagact ccccatgatt
    12061 gaccacgtgc ccagcatgaa tcatcactat acccttgtct cgttacacct ttaaagggtt
    12121 gacctgtctc aggatcaaaa tagaaggtgt ggaaggacga agcgtcatca cggattacat
    12181 tattagctga agcatagaaa tgactttctg caatatcgta gtatttttga tcgcctgttt
    12241 cttgataagc aaagaataag agttggatat tgagcaagca gtcgataatc aaacggtaat
    12301 gctctttctt gcccaagtct ccccaagctt gaataaaacc acctttttct tgatagcgtt
    12361 caatcaactt atctgcagct ttcaaggttg cttctctagc ctctccatct ccatttatct
    12421 tatattcagc catacaagac ggtgtgtaca agaagccgag atcatggtga tccaattcta
    12481 ctctcttatt gacacgatcc aggaaagaaa gaacattttt atgagcgatg tttttaaatg
    12541 catcctgttg actgtattca taagccaacc acagttctcc tgtccagaaa ccattggtcc
    12601 attccgtgtt atccatgatt ggatagacat tatcaaaggt agctggcgtc gggaaatctt
    12661 ccttgaaata gtcaaggttg agttctaact gccgaataac cttatcgatt gcctggccga
    12721 cttcttcttt cgtcagaagt ggtacttcta agaagcgctc aggcgatttt attttttcaa
    12781 tcgtaacctt ttttatcatt cttttaccct ttaaataccc gattcgaaat cttcttcctc
    12841 ttcctctgca tccgttgaaa acaattcttt accatttacg actccgccct gggccgccac
    12901 tactgattta ttaacaacct catcaaagct atgagccatt ctagcaaaga ctgcttccat
    12961 taacatggct aagttcaaac ccgagagaac attcattgtt ttggctggat tttctcccat
    13021 tatggtagaa gaaaccttga atggcgatcc tcccaagaga tcacttagga ttaaaacttc
    13081 ttcttcattt gaaattgcaa gtaagatttt ttgcttgagt tcatctgctg acattccttc
    13141 cacaaagtca attgcctcca cattttcttg attgcctgca atcaattgta aagaactata
    13201 aatccctgta gcaaaatgtc catgccctac aagtacaatt ttcatctttt ctcctttctt
    13261 gtttaggttt accggtttac tttatattcc ccattgtaat cctttatttt tatcttgtca
    13321 atagtttttt taaaaaaata ttagcgcttt cattatcttt ttaatcattt taatagacag
    13381 aaatttcaaa atattccatt tttaaggaaa ataaatcttt ttataaataa aaatatttat
    13441 ttattgacaa aaccggttta ctagaatata atttggttat agagaagtct taacaaattt
    13501 gaaaggaagt atcaaatgac aaatacatca ttctcaattg agcagttttc tttaaaagga
    13561 aaaattgctc tcatcaccgg cgcttcttat ggaattggat ttgctattgc caaatcctac
    13621 gctgaagccg gcgctactat tgtctttaac gatatcaatc aagatctggt caataaaggg
    13681 attgaagctt atcgtgaagt tggcatcgaa gcccatggat atgtctgtga cgtgacagac
    13741 gaggacggta tccaagccat ggtcaagcaa atcgaacaag aggttggtgt cattgacatc
    13801 ctcgttaata acgctggtat tatccgccga gttccaatgt gcgaaatgag cgccgctgat
    13861 ttccgtaagg tcatcgatat tgacttaaac gcaccattta tcgtttcaaa ggcagttatt
    13921 ccttctatga taaagaaagg gcatggaaag attatcaata tttgttcgat gatgagcgaa
    13981 ctgggacgtg aaacagttag cgcttatgct gctgctaaag ggggcttgaa aatgttgacc
    14041 cgcaacattg cgtctgaata cggtggagcc aatatccaat gtaacggaat tggaccgggt
    14101 tatattgcca ctcctcaaac agcacctctt cgtgagttgc aagaagatgg ttctcgccac
    14161 ccatttgacc agttcatcat tgcaaaaaca cctgctgcac gttggggaaa tcctgaagat
    14221 ttgatgggcc ctgctgtctt tctcgctagt gatgccagca attttgtcaa tggccacatc
    14281 ctatatgtag atggcggtat cttagcctac atcggaaaac aacctgagta aaaatagaaa
    14341 gaagatctta tgaaaatcgc attaatcaat gaaaatagtc aagctagcaa gaatcacatt
    14401 atttacgata gtctaaaaga agcgacagat aaaaaaggct accaattatt taactatggt
    14461 atgcgtggag aagaaggaga aagtcaatta acttatgtgc agaacggact aatggctgcc
    14521 atccttttaa atacaaaggc agttgacttt gttgttaccg gctgtggtac gggtgtaggg
    14581 gctatgcttg ctttaaacag cttccctggt gttgtctgtg gtctagcagt ggacccaact
    14641 gacgcttacc tttattctca aatcaatggt ggtaacgcct tgtctatccc ttatgccaaa
    14701 ggatttggct ggggggcaga actgaccctc aaattgatgt ttgaacgctt atttgctgaa
    14761 gaaatgggcg gtggctaccc aagagaacgt gtaatccctg aacaacgcaa cgctcgtatc
    14821 ttaaacgagg tgaaacaaat cacccacaat gatttgatga ccatccttaa aacaatcgac
    14881 caagacttcc tcaaagacac catctctggc aaatatttcc aagaatactt ctttgaaaac
    14941 tgccaagatg atgaagtcgc tgcttatttg aaagaagtat tagccaagta aagctattct
    15001 aaaccagaaa ggaactaatg gatgacgaaa atattactgt ttggcgaacc attaattcga
    15061 atctcaccat tagatgccac cagtatcggc gatcatgttg ccagttcgac ttattttggc
    15121 ggatcagaaa ttaacatcgc ttgtaatttg caagccctgg gtatctcaac gaaagtcttt
    15181 accgcactcc ctgccaacga gattggagat cgttttctca cattcttgaa acagcaccaa
    15241 atcgatacca gttcaatctg tcggcttggc gatcgaatcg gcctctacta tttggagaac
    15301 ggctttggtt gtcgtcaaag tgaagttttc tacgatcgta agcatacgag tatcagccag
    15361 attcggccaa acatgctaga tatggattct ctctttcagg ggattagcca ttttcatttt
    15421 agtggaatca ccgtagctat cggtcaagag gtccgtgcga tccttctcct actcttggaa
    15481 gaagccaagc gccgaggaat tgtcgtttca atggatctca atctgagaac aaagatgatt
    15541 tcagtcctag aagccaagta tgaattttct aagtttgcac gttttactga ctattgcttc
    15601 ggtattgatc ctctcatgat tgatgaccaa aatctagaga tgtttccaag agacagtgct
    15661 agcctagaag aggtggaaaa tcgcatgcga cttttaaaag aagcctatgg tttcaaggcc
    15721 attttccata ccctccgctc tagtgatgag caagacaaaa atgtctatca agcctatgct
    15781 ctggaagaac gatttgaaga gtctgtccaa ctaaaaactg cagtctatca acgaattggt
    15841 agcggggatg cctttatatc tggtgccctt taccaactac tccatcattc ctccctaaaa
    15901 actaccattg actttgcagt tgcgagcgca actctcaaat gcactcttcc aggagaccat
    15961 ctctccactt ccgcaactag tattgaaaat ttactggcaa atgcacaaga tatcattcgt
    16021 taggagaatt acatgaccaa atcagatacg attattgaac taaaaaaaca gaaaattgtc
    16081 gctgtcattc gaggaaatac aaaggaagaa ggactgcaag cctcgattgc ttgtatcaag
    16141 ggcggtatca aagctattga aatcgcctat accaatcagt atgcaggaca aatcatcaag
    16201 gaacttgtag acttgtatca ggacgatcag agtgtttgta tcggtgcagg tactgtgctt
    16261 gatgccgtaa ctgctagaga tgccattcta gccggagcaa attacgttgt ttctccatct
    16321 ttccatgctg aaactgcgaa aatgtgcaat ctctacagca caccgtacat tccaggctgt
    16381 attaccctca cagagatcac gactgcactt gaagccggta gtgaaatcat caaactcttc
    16441 ccaggtagtg ctctcagtcc agcatatatc tctgcagtca aggcaccgat cccacaagtt
    16501 tccgtaatgg taaccggagg agtcggtcta aacaacatcc ctcaatggtt cgctgctggt
    16561 gcagacgccg ttggaattgg tggcgaactc aataaactcg cttcccaagg caactttgac
    16621 cgcatcagcg agattgccca acagtatgtt acactcagat aaaatcataa ccacccgtca
    16681 aacgggtggt ttatcccaga gctataagcc caaattacca gccagcgcct aaagacgctg
    16741 gctttcacgt tgttcaagcc ttattgctct tgactcgtca cttgcctctt taagagactt
    16801 tggtattact taccactatc cctaaaggga tcctcatatt cttttacact caatttatct
    16861 agt
//
LOCUS       DAVBCG010000033        16704 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBCG010000033 DAVBCG010000000
VERSION     DAVBCG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.033"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..473)
                     /locus_tag="VQ495_000903"
     CDS             complement(<1..473)
                     /locus_tag="VQ495_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2530881.1"
                     /translation="MTIEPGQRIALVGQSGSGKSTLSKILSGLYKIDTGKILFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKY"
     gene            complement(642..1955)
                     /locus_tag="VQ495_000904"
     CDS             complement(642..1955)
                     /locus_tag="VQ495_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2530882.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYT"
     gene            complement(1948..2496)
                     /locus_tag="VQ495_000905"
                     /pseudo
     CDS             complement(1948..2496)
                     /locus_tag="VQ495_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2577..2639)
                     /locus_tag="VQ495_000906"
     CDS             complement(2577..2639)
                     /locus_tag="VQ495_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2530883.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            complement(2808..3796)
                     /locus_tag="VQ495_000907"
                     /pseudo
     CDS             complement(2808..3796)
                     /locus_tag="VQ495_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3745..4872)
                     /locus_tag="VQ495_000908"
                     /pseudo
     CDS             complement(3745..4872)
                     /locus_tag="VQ495_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(5035..5919)
                     /locus_tag="VQ495_000909"
     CDS             complement(5035..5919)
                     /locus_tag="VQ495_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2530884.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(6031..6885)
                     /locus_tag="VQ495_000910"
     CDS             complement(6031..6885)
                     /locus_tag="VQ495_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2530885.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(6943..7701)
                     /locus_tag="VQ495_000911"
     CDS             complement(6943..7701)
                     /locus_tag="VQ495_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2530886.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            complement(7763..7951)
                     /locus_tag="VQ495_000912"
     CDS             complement(7763..7951)
                     /locus_tag="VQ495_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2530887.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(8273..8839)
                     /locus_tag="VQ495_000913"
     CDS             complement(8273..8839)
                     /locus_tag="VQ495_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530888.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(9107..10786)
                     /locus_tag="VQ495_000914"
     CDS             complement(9107..10786)
                     /locus_tag="VQ495_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2530889.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            complement(10878..11039)
                     /locus_tag="VQ495_000915"
     CDS             complement(10878..11039)
                     /locus_tag="VQ495_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530890.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(11723..12037)
                     /locus_tag="VQ495_000916"
     CDS             complement(11723..12037)
                     /locus_tag="VQ495_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530891.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(12003..12392)
                     /locus_tag="VQ495_000917"
     CDS             complement(12003..12392)
                     /locus_tag="VQ495_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2530892.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(12780..13574)
                     /locus_tag="VQ495_000918"
     CDS             complement(12780..13574)
                     /locus_tag="VQ495_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530893.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(13574..14581)
                     /locus_tag="VQ495_000919"
     CDS             complement(13574..14581)
                     /locus_tag="VQ495_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2530894.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(14581..15588)
                     /locus_tag="VQ495_000920"
     CDS             complement(14581..15588)
                     /locus_tag="VQ495_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2530895.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(15578..16603)
                     /locus_tag="VQ495_000921"
     CDS             complement(15578..16603)
                     /locus_tag="VQ495_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2530896.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT         5229 a         3150 c         2259 g         6066 t
ORIGIN      
        1 atatactttg ttattctttc ctcattaata gtgtctaatg cactagttgc ttcatctaaa
       61 attacaatac taggattatt tattaatgca cgtgccagtg ctatccgttg cctttgccca
      121 cctgaaatat ttgaccccat ctctgagatg atagtattaa atttcatcgg catagccatg
      181 atttcatcat agatttgaac tgctttacaa acttcctcta tcttttgtga agtaacttcg
      241 ttctttaaag ttatattatc aagaatactt cggttcaata aaaatgaatc ctgtggaact
      301 actcctaaat tttgacttaa tattttttta tctatttgat taatatttac accatcaaat
      361 aaaatttttc ctgtatcaat cttatataat cctgatagta tttttgataa cgtacttttt
      421 cctgaccccg attgaccgac tagtgcaatt ctctgtcctg gctcaatagt caaatttaaa
      481 ttttctatta caggagctga gtctttggaa taagagaaag ataggtcctt aatatctatt
      541 ctaccttcta aagaacactt tttaagacca ttttcaacat tctcttcatt ttcaagccag
      601 atatcattta ctctatttaa gtatcctgct gccaaaatat actacgtata agcattaaat
      661 attgagattt ctgaactaaa taaaattgat gccatcgttt gaaatgcgac aacctcaccc
      721 aataaaattt ttccatttaa aacattatca agtccaaaaa tcaatataat tataggagcg
      781 aacaattgaa atacattagt agctgaattg tataaaatat gcacaatact tcgtttttta
      841 tactttgtca atacgttttt taaatactta gaccagtttt catatatttc atcttctaat
      901 cccgatattt ttattgaaga aatagtattt aatgcttctg tttctactga gtaaatcaat
      961 gattgttctt gtaattctct attaaccgta tcatacatta tttttctagt actaaacaga
     1021 aacaaaaaat tgattaaact caatataaga gctataattg ataatgtttt tgaactaaga
     1081 aacatatata ttacaacact tagaatagtc ccgatatcta ctactccccc aacaacttgg
     1141 ttagtaaata gctccctaaa accactaaga ctagaaattc tgtataaaat atctcctttt
     1201 gccctgagtt caaaaaaact atatggtagt ttaagcaaat gcttaaatgt aaaactttca
     1261 atatctctac taaaaaatat attagaaaat agaattccca tactcctagc caagatagaa
     1321 ataaggtaca aacaaagtag agaaaataaa attaaaaacg aggtttgaaa agaaaatgta
     1381 ttccctaatg cagaattaat aaactttgaa gtcattactg gtacggaaag tattattaag
     1441 taacttatta ttgaaaatag taacgtcaaa atcaatcgac cttttacctt tgaaaaacta
     1501 gagaatactc ttatccatgg agaaggaaac tgcgacttca aagattgata attttcgtta
     1561 ggaaatgata gcagtactat attactaaaa tgttttgaaa actccttaaa ctcataattt
     1621 atatatcctt tagaaggatc cattatatag accttattct tttttacttt gtaaataact
     1681 acataatgtt gattatccca gaatgctatt aatggtaacg gaagttcact aattttattt
     1741 acatctttaa cttgatatga gccagtatca aaatttcgtt tattcaataa ctgtttcaag
     1801 tcacctatac tataaccatc tctccctttt tctatatcac gtcttaaatc tagtaatgtc
     1861 tctttgcttt tatagaaatg tagtatggac aagcagcaac ataaaccaca ttctatttga
     1921 gacatttgct caatattagg tacttttcta gtcatcatgt cctcttaact ccagtaataa
     1981 aacattaagt aatctatcat cttgttttcg agatatttca tagacaactc cagatactcc
     2041 tagcataaaa gaaaatgtat tcatagtatt caaatccgta cttaattctg gtttgataac
     2101 ctgagaaacg acattatcaa aataaaaatc tatagattct tttattaata ggttttcttc
     2161 atctttcaat attgcatcaa gacaactgat agtacctaca gaaccgtgac ataaattcaa
     2221 ttctcttatt ttttctacat tagttaatac attttcaatt attgataaat cattattccc
     2281 aatattagtt ttatcgaaaa tttcttttct agctaataaa atgcccggag ctccataaca
     2341 ccaaaaatta ggaaaggaat cattatgatt tcttcatatg tctttccaat tttttaatat
     2401 attattgaaa catttatctt ctttatatag taattcctct atacatttta atatatctgt
     2461 actaggatta atttcataat aatatgataa ttacattgct actccagaat aatcgtgtgc
     2521 taaacctgtt actgttatat tatctaattg tgaaatacaa taattaatac acttttttaa
     2581 atgatgataa tatccatcac caaaaccaaa atcggtaaaa attttgaaat tttcttcata
     2641 cttggacttc ctttccgttt ttatttattg tacaataaat aaaaacggat ttaatttttg
     2701 ttgggaaaat gggaaattat gaaatcaaaa cttggcatca cacttagaaa agttcgtaaa
     2761 ggaaaacaaa ttagtctatg ctctgtagca gatgaacatc tttcaaaatc acaaatatct
     2821 cgttttgaac gtggggagtc agagatatct tgtattcgac taatcaatat tttagacaaa
     2881 ttacatatta ctttgtaggg gagtttttgg ctatcgttgc tcaattgtct gattaaggtc
     2941 ctacccttga tgaaacaatt attatccatg ttttctttat tatagacaaa gtaagaagac
     3001 gtttctcgaa tgtagacttt atatttttta tgattttctt cttccataat atccaattga
     3061 tagttgggaa tgaaaataag accgctctgt ttgacaccga aagacacctt gatatagacg
     3121 cccttatcaa ctagcttctc tatttggttc tctgcaagtt ccacttcaaa ttcacgaacg
     3181 gtatctcatt tttccttaaa tgtcttaaag gcttcctcaa tctcttcagt ggatacttta
     3241 tccttatctc gttcttcttg gaaagcatgg tactgttcct gtaaattctc taatccttct
     3301 gaagcaacga cttccttatt tttaaaataa tcttgaaaaa atttgacatc atataatttc
     3361 ttatcactta ttttttgatg acccaaactt atcttttgat tattttcttc caggataaaa
     3421 gttacatttt ttgttttaag tcaatggtta gattcaattc ttttgctttt gttattaaat
     3481 cttctaaaga attgacacgg tttaacaaaa attctaaacg actttcaatc tcttgcttag
     3541 caaaatgcgt tctaaaaaat tcttcatcat atagatctcg tttgctgagt tggcgccctc
     3601 gaattggttt tatcatcgtt ctatctgtca tcaaaaaacg gctatgcttt tgactaaaat
     3661 caatctgaac atgcaactgc tttgctttct ctaaaaaatc atcaaacgat ttagattgct
     3721 gaagcaaaaa ataaagacgt tgtttcaatt caaatttatg actagattcc ttatattttt
     3781 tataatctcg aaaagatcag gatttttagc taaaactgcc ttacgaaaat ccttccagaa
     3841 ctgatggtca atctcattag ccacatccaa acgccaagca tcgatattaa actcttcaat
     3901 ccaataagtc gcaaccttta aaagataatt cttgacctct ggattggctg tatttagctt
     3961 aggcatatag tcctcgaaac caaaaacatg atagggtaag tctctcttat taactagctt
     4021 ttcagttgtc aatgggaatt gttgaatatg gaaccaatcc ttataagcag actgttcacc
     4081 atttttgacg acattttttc attgaagaga ttgcgaacca atatgattaa ataccgcatc
     4141 cagcatgact ttcatgccac gatgatgcgc ttgatccacc agttcccgaa aggtctcctt
     4201 gtctccaaaa taacggtcaa tttcaaagta atctgtcgta ttgtacttgt gattacttgt
     4261 agattcaaag atgggacaaa gatatagtcc agtaataccc aagtcttgca agtaattcat
     4321 atgatcaata atcccctgta aatcaccacc aaagaaatca tcgctcttag gtgtgacaga
     4381 tgaatcccag tctaaagtcc cttctgggtt taatagagca ttgccattgg caaatctttc
     4441 aggaaatatc tgataccata ccgtatttga aacccagtca ggaaccttgc aggcatcaat
     4501 ctcatgaaga taaggcaact taaatccatt cccaattgca tgaagatttt ctagagaatt
     4561 ttccacacac cctttatcgc catacaaaat attttgacct tctgtatctc tgagctcaaa
     4621 gagatactgg atacgtgcaa agtcaactga cacttcaacc tgccaatggt caaataaggt
     4681 accagaagtt atcttgacca tttcttttgt atcctgataa aactcttcca taaagataaa
     4741 agggtcccca tagtgcaagt tgatgctttc aatgtcccct ttcttagttc gaattcgaat
     4801 atggagtttc ttatccttat aaagataggc atactccgac tcaggcctat ggtaaatagc
     4861 actcaattcc atcaatttgt ctcctaaagt attcattcaa attttacgca aacgttttca
     4921 aactcaataa atccagtata ctatttttgt aaactatttg caaggaatgt ccgttgttaa
     4981 aggaactatc ttttaaagta tacaaaaagc cttaaaaaag gctttctcta tatactaatc
     5041 ctctttcggt tttgaaaata cttctactcg ctgagctagg actttgattt ctacaggtag
     5101 gttatctgat ttatctccgt caacatctga ctccaattca ctatctgaag agattttaat
     5161 attacgcgct ttgatatact cgatattatc atttgcgaca acatcccctt ttaataaatc
     5221 aggaatgacg gataatttgg agaatataga ggcatctttc agaatcaaaa tgttggcata
     5281 gccgtccttg ttttcttcaa agattttctt atcagcgaag taatttgtca aaaggaccaa
     5341 aacatggcta gcttcaccaa cataatttcc attttctgtc tcaaccttaa tgttaaagac
     5401 ctgatctgtc atgacagact tcatggtatt tacagcatag gttaaaatac cgaattttgt
     5461 tttgtcctct atttcaacat tgtgaatcgc ctcaggcaga gaaccgatac taaagatata
     5521 accaaaatag ttgtcatttg ctttaccgat atcaatctta ttggttaaat caaaatccag
     5581 ttcgtcaatt gcgccatcga tgtcttgatt gatttccaaa agttttgtaa tgaggttacc
     5641 cgtaccgcct gggataatcc ctaacttagg aatgtagtct ctctcatcaa tacctgaaat
     5701 gacttcattg acagttccat ctccaccaaa cacaaccact gcatcatact gctcacgaga
     5761 agcttcttca gcaaaatgtg ttgcatccag cgctttttcg gtaattttgg tttcaacata
     5821 ttcaaagtat tcttttgctt tattctccag cttttctttg taatccaaag ccttctcgcc
     5881 accagaagta gggttgataa ttaccattgc ttttttcatt gattttatcc ttaattttaa
     5941 acagaaatgt ttacatttcg tcgtatgcaa gtaaatgtaa tcctattata caatgaaaat
     6001 acagaaaaga gaaatctgac gtactggaga ttaatacgct tttattctat tttcccatcg
     6061 cctaactaca tcctttaagg gttcatccaa gtaagaatag gccttatcct tgatccaatc
     6121 aggaataccg taagctgcct ctgctatgtt acaagtgatt gctgcgagag tatcactgtc
     6181 gccaccaagt gagatggcat ttcttatcgc atcttcgaag tctctacttt caagaaaggc
     6241 gataatggct tgagggacag tttcctgaca tgtttcgtta aaacgatagt taggacggat
     6301 ttcatctaaa gtttgagata gattgtaatc gtattctttt tctatataat ccttaattcg
     6361 tcttttacac tctgtaagat tgtcgttaat tgattgctcg tactctctac aataaccacc
     6421 aaagtaaaaa cgacatagaa agatagcatc agctgtagcc atagcgcctt tgacaccttc
     6481 tggatgatta tgagttacct ctgcagaaag actcgtaagt cctctagatg atggccatat
     6541 accagttttc gcataaaaac cacagtccat gatccaagca catggagaaa tacgcatagc
     6601 tgatccattc ccaaagctat tataaggctc acggttatcg ctgtttagcc atgcattaaa
     6661 ccgagcaccg taatcagcat tcggatacat tctgccatat ttcttcatcg cgtcaatgaa
     6721 atcgtctttt tgtccaccat tcataattgc ttctgcaaca gcacaggtca taaccgtgtc
     6781 atctgtaaaa aagcagtcct tccgaaataa aggaaagtcc tttgttttga tattgttcca
     6841 ttcgtaaaca gaaccgacaa tatctccaat aattgctcca agcatcagat tcctccttgt
     6901 tcattttgat gctttttata ttggttatat accatattta ttttagaaaa caacatcctg
     6961 ttggatttta aaaatttcat ttttttcaaa atagggtttt accatttctt tccacctagc
     7021 tctatgaaaa ttgattgatt ttaaaggaga tagggcataa tttcccaatg cataaccatc
     7081 atttacttca acaacaagtg ttctgccatc gcgagtaaca ccgatatcta gtccataagc
     7141 tattggcgca tctttccaac atgatatcgc ttcatcaatt acacttgcat caaattgtgc
     7201 atgataatca cctgtatagg gtcgaacatc taatacgcga ccatctaaca caaaacaacg
     7261 ccattcagct atgaattcta caacctcact aatccatata ggatagtcga aaggtagacc
     7321 aatacctatt aaatcatggg ttccattaac aactcttcca gtaaagactt ttgaaccagc
     7381 tttaggctta ataaattttc cccaattatc aggtatattc acaatctctc ctaaaatacc
     7441 agcataaatc tttcgaccat aaaactcttt aagctcaata ggatagtcat aaaccggaac
     7501 gtttaagccc atcattttta gtaatgctct agtctccatt atataatcta caactatatc
     7561 ttcacttgtt aactctttta tttcagaaaa agattgatat aaaataactt cttctccttg
     7621 taagtaggca cctacttgag cattgtattt attaattgaa acctcacttg gtaatttact
     7681 ttgtctaata taaacaacca tttcatcact cctatatcac tagtgttaca ctgatttgta
     7741 aaaaataata gcaattttgc tcttattttt ttgagtaaat agctcccata atatcatcga
     7801 aataatcaac ggtatttagg agtaattcaa taacctggga ctttgttagt cgcattcccc
     7861 ttctatctct agcatcttct actaaatttt caagtttctc tagattttta tcatccaagc
     7921 taatcattat tctattttta tcggttgcca ttttcatcac ctcaagttaa ttctatcaca
     7981 ggtgtaacac ttgtgtcaac tggcttttat aatacattag tttaaaagtg gagaggattt
     8041 ttaacacagt aactttaaat ctttggtatt aaaaaatttt cacaatattt atagaaataa
     8101 aatctgtctc aaatcagtta tcaaatctag tataaattat gagcggctac tctaatactt
     8161 tccctctaaa caagaaaaag acttacactc aaggattttc ttccccccct tcgttataac
     8221 gttttgactc ttttactagc aaaggtatat actcacaagg aactttggtt gactattgaa
     8281 tctctccaac ttcttcttta acatatcctt ctacatcttc aatctctaca aacattgggt
     8341 ctaagtgaca caagaaatgc caaacttcga tccctttttt tctgtaaaga atcgcttcac
     8401 cgtcttcact tccgaaaaag cttctgtcga tttcatatcc gcggctttct aagaagtctt
     8461 ttgctttacg atagttcgtt tctcttgttt cgacataggt tttaacttca tggttgttaa
     8521 cgacatatgc atcaattttt gaatatcctt cgatcactct atcatttttg agggataaat
     8581 ttgaaatctc tttccaaata atgtttacat tttcctcagg atcgaacata aatttagata
     8641 aaggaacaat atttccgtta aaaataattt ccatataatc cggtatgttt ttaggattaa
     8701 aatactccac ttcaaaacca tcttctgttt ccagagtgta tcccgggatt tgagctacaa
     8761 aggctttccc atcttctatg gaatcaaatg ctactaaatc tttagaataa tcattttggt
     8821 acaattccaa tataaccatc gataatctct ccattttcat tatcaggcta atgtaaataa
     8881 gcacgtcacc tgaccaattc aggctctctg tatcatctca tcatatttcc tacttacttt
     8941 acgagtccta tacccagaac acaccttatc gaccttcggt ctcacctcgt tgcattggct
     9001 gaacatctac ttttacttcg ctgatgcttc aactcgtaca agcagtgata ccgcctcagc
     9061 gtgatgcgtc agtgggactc aaaaggttcg gggaaccttt tgaggattaa ctacgtttct
     9121 ctaataaact tacacattca acttgttcat cattgtccaa acctatgttg agattttctt
     9181 ctataattgg tagcttaaaa gtaatggatt ttagccattg tccgttagat tgtttttctt
     9241 cataaacttg aatttcagaa atcaaagctg aaattaactg cctacgctct acatcattca
     9301 tgactttata gagcttatca aaatagatca gaaccttata tatgttatct cctgtaagct
     9361 tttcagcttc aatagtctgt ttctttgctt tcgcatcaat tagtgatgat tctaattcat
     9421 ctactttgtc atacatacga tatagtctat catctaaatc ctgtttcctt ctcttataat
     9481 gcttatcttc aacatctaaa ttatctattt cctcaattag cttaaacttt gtagaatgac
     9541 tctttctcaa ttccttttgg taattatcta tttctttttc tatttcagag gtatccacct
     9601 tcatgttgat tttttcttgc atcatagaag caaatttcgg attacttact atcttgacaa
     9661 tcacctctgc aacagcatca tctaacaatt cttctctaat ttgcttactg aatgtacact
     9721 tatgacctct tatcatctgc ctatggttac aaccatagta ataaaaatct ttatactttg
     9781 tgccatcttt ctttttcttg atacacttgt tcccaaacat tcccactcca catatcgggc
     9841 attttacaat tccagaaagc aagtgtgtgc gtgtatcttt tcctttattc acatgctcat
     9901 atttctttgc ttgagatttt agcttaacct gagcagcttg ccaaacttca tcggaaacta
     9961 tagcttcatg tatcccttca gatattagat attcatcttg ttcaacctgc ttatattcat
    10021 ttcttgtacc atgaactttt tctaaagttc ttcttccaaa tgctattttc ccattatata
    10081 caggattctt taatatcttt cttataagac ctgcatcaaa caaaggattc ttaccattct
    10141 gtcttgggat ttttctaatt ccatgattct ctaagtattt agatatccca ttggctccta
    10201 tcgtagtatt tacatactgg tcgaaaatcg ttcttattgc aactgcctct tcctcattta
    10261 taaacagctt gccgtcttca agtttatatc catacggagc aaagccacca ttccattttc
    10321 cttcccttgc tttttgaatg cgaccttcca ttgtttgaat acggatgttt tctctttcta
    10381 tttcagccac agctgataaa acagaaatca ttagtttccc agcatcttta gatgaatcaa
    10441 tgccatcttc aacgcagata agattaactc cataatcctg cattatctga agtgtagaaa
    10501 gaacatcagc ggcatttctt gcaaatcttg ataacttaaa cacaagaaca aaagatactc
    10561 catcttttcc agattttata tcttccatca ttcgattgaa ctgtattcta ccttcaatag
    10621 acttgccaga cttcccggca tcttcatact ctccaacaat ttcataatcg ttgtaaatag
    10681 caaaagcttt cattcgtgat ttttgtgcct ctaacgaata cccctctatc tgtattgacg
    10741 tagatactcg tgtatagagg tatactttta ttttttcttt tgacatagta ttaatctcaa
    10801 tataattttt ctatatcata tataattttt ttaatttaag tttggactat catttcaagt
    10861 atattataac acttttatta gtccgtctca atttgtgttt ttgccatgtc aaaactattt
    10921 ttcatctctt gattttttgc tggcgttgga tcgggtagat tatctaaatc taaagcacca
    10981 gcatattttg caatcagatt tgctattaaa tcagccaatc cattccagtc attgtccaat
    11041 atatacctcc tctaaagttt tatatctaat aattatttgt ttaattaagt tttttgacat
    11101 tgacaagtgc tttggattag caacatagga atctcacctc cgcctctatt ccggatgagc
    11161 cggcttcaac cttagaagta tcattatcct cattttcttc atagcggata gggtatccct
    11221 ccctatattc aaactcttac ttatcgctca ctttcttttt acttagcaga actttttttg
    11281 ccgaattatt cagccgaaag atcttgacgg ataggttatt acgctccaaa aataattaac
    11341 gtcttgtctt ggtctattca attgttaagg ttcaaaattt atcgagagtt attaatcttt
    11401 ttaaaatttg accatcagaa aatatttatc ttaatgtaac aaaattctat aaattaccct
    11461 cttatactta acagtgaaaa gaagtctttc ttggtaacca attttgaaat agaatttgct
    11521 tatataaaaa ggtcaaattc ccactgcata agtagcagtg aaaattagac cctcttggta
    11581 actgtcatct aaaagtcttc tagcatttct ttaattttct aatcgccttt aaagtgtcat
    11641 atttatattt ttttatctct ggcatacttt gatacctttt tagacttaaa gtctttaata
    11701 gtgcctttcc acctcttttt atctataaag attctcctac atcataattc atttttttat
    11761 ttaaaccttt ctgtcttagt ttgtctttat cttcttcata ccattttaag attgtcacat
    11821 agtggttttg ataggtctta ccactgcttt ccatgtatct ggatagttta tttatcatta
    11881 tatctgtgtg tgagtttaat ttttctttta gatttttata ttcttctttg cttaacctta
    11941 catttttgaa ttctccataa aaaatggggg tggacttttt atctatctct ccctctctct
    12001 ctttatctat ctctatatct ttccatgtaa ttccaatctg gagtacctct actgtctatc
    12061 ggtaatttaa ttttgatatc tggcaatact gtgctagata tttgatcttt atattcagta
    12121 ttttttaaag cttgcctaat aattgaagtt aaatagaatg ctacttcttt attcaattct
    12181 ttatttttta attttaaaca atgaattttc atatctaggc ttgctttata tttatgataa
    12241 aagactgctc ctaaaaatga aacagatata aaattttcaa aaactctata atttttatca
    12301 tctatatctt cgtagtaacc taagatacca ttgtcaatat ttgtagcact aattctagga
    12361 gtttttccat cgagtaaata tctttttgga atagatgagc ctgttggtac ttgactcgat
    12421 ttcccctttt tttcggtaat aaatatttct ttttattttg ttgtctgata tttttcctac
    12481 ctgtcctttg taggatgagt attttctaga ttttcttgaa taacttttta cttgaagttt
    12541 tagcttttga actagtcgtt gtactttctt tttgtttatt atcagtcctg atctttttaa
    12601 tattgctgtt attctctata tcctattttt cattcatgat attcttttac taattttatc
    12661 ttaaattctg tgctgtattt gccattaaaa aactgacctc ctttagttag ttttttggcc
    12721 taacttttga gggtcagttc aaaatttgcg acttttaaat gaattccaat attcaattat
    12781 taagagctaa catggtgctt gccaatagga atcattagag gcgaattgga aatagggtca
    12841 cgtataattt ttgcttcaag attaaagata tctttaacta gtttatcatt tagtatatct
    12901 tcaggctttc cctctgcaac aagtttacct tctttaattg caaataggta atcagcgtat
    12961 cttgctgtta gatttatatc gtgcaaaatc atgcaaatgg ttgtcttata tttttggttt
    13021 agatcagtca agaggtctaa tagttctatt tgatatgaga tatccaagta agtagttggc
    13081 tcatctaaaa gtaggatact tgtatcttgg gctagggcta gagctatcca tactctttgc
    13141 ctttgacccc cagaaagttc ttcaactagg ttatttgcta gatcttcaac attggcctta
    13201 accattgatc tgtttattat ttcaaggtca tcttttccaa gactcttaaa aggctttctg
    13261 taggggaaac gaccacggct tacaagatca gctactgtta ttgattcagg gattattgga
    13321 gattgaggta atatagctat gtgttttgct aaatcttttt ctttataaga attaattgat
    13381 ttattatcaa gcaatacttc tccctctaat ggctttataa gtcgagacaa ggttttaatg
    13441 agtgttgatt tcccacaacc atttgaccca ataataactg atattttttc ttcaggtatt
    13501 tttatattta tattttccaa gattattttt tcatcataac cgcaggtaag attatttgac
    13561 cacagacctt tcattatata ttcctcctgt tcatttttat tagtaagtat attaagtatg
    13621 gtgaacctaa caagccagtt acaacaccta ctggatatct agctggtaaa atattttgag
    13681 agaatatgtc tgataacaaa actagtaaaa ttccaaccaa tccagctaat attgggcttc
    13741 ttttcttgcc aatatttaag gctatgggac cagctaaaaa agatatacaa gctattggtc
    13801 ctgtaattga agtagaaaaa gcagttaaag atacagcgca aaaaattaaa acaagccttg
    13861 aaagctcggg atttgctcca agtccgattg ctatttcttc accaagttca ataatttcta
    13921 gtcttttatt aaaaaataaa actaatatag tagcaataat acttactatt agaacaagag
    13981 gtatgtcatc taactttgta aaagataaag agccactgag ccatctcata acttcttgta
    14041 attcatatct tgctactttc aacaataaaa atgaggtgcc tgctcttgtg acagcttgaa
    14101 aaccaatacc taatattatc agtcttgctg ctgaaaaacc atctttttta gctagtaaaa
    14161 ataatattaa agatgatgtt agtccacaag ttattgaaat aattccagta gttaaactat
    14221 ttgtttttaa taccaatatg caaaagaccg ctgcaataga tgaagaactt gtgacaccga
    14281 ttatatcagg acttgcaaga ggatttctta acatagtttg aaagataaat cctgccaatc
    14341 caaaagacca gccagctata attcctgcta ataattttgg taatctaatt tccataatcg
    14401 aaaaactagc tccaggaaca gtttcactat ttaagacttt aatcaaagtt gaaaaagaat
    14461 aactttcatc tccgataagt aaaatgaaaa atgatagact gattattatt aataaaaata
    14521 gtgaggaaaa tagtgttatt ctattttttc ttttttgaat acctataatt aaattttgca
    14581 ttagttatta acccctctat ttttcatagt tacataaata agtactggac ccccgattat
    14641 tgcagtaatt atccctactt caatttcacc tggtttacct aacatacggc cgattatatc
    14701 acatataagc aagagctctg cacctataaa agatgaagaa atggtcattg tgcgtatatc
    14761 tttgcttata aataagccac aaaagtgagg aactataaga cctacgaagc caataggtcc
    14821 accaattgca gtaatacttg aacataaaag cacacttgca attattgcaa gtgatcttat
    14881 cctattaaca ttaactccaa gaccaacagc catttcatca cccatagcta aagcgtttaa
    14941 atctgatgaa ataaatatag ctatcaagtg acctaaaatt ataaaaggta gtagtgtaga
    15001 tatagaagat aatgtagctg ctccaaggct acctatttgc caaaatctaa atttgtctaa
    15061 gacgttatta ttcggtaaaa ttaaaaaact tacaaaactg cttaaagcca tactaacaca
    15121 agttcctgat aaggctagtt ttataggggt aaggcctgct tttccgctta cagcaatcgc
    15181 gtatacaaaa attgcactta ctaagccacc aatgattgca aaacttatat ggcttatgct
    15241 tgatgaaatt cctaaaaaag aaagaccaat tactacacta agacttgctc ctgtgtttat
    15301 accgagtata cctggatcag ctattgggtt tctagtaact gattgcatca atacaccgct
    15361 tatggcaaga ctagaacctg ctaaaattgc aaaaatagtt ctaggtattc ttttatatat
    15421 aattgattta ataaaatcat catttgtatt gcccattcca aaagttgcta aaaaatctct
    15481 gatattaatt tcttttgttc caagctttaa tgacaaaaat attgtaataa gaagacctag
    15541 gcagattaca aaaattatgc ctaggtttat ttgtttttta tttcgcattt ttgcatgcat
    15601 ttcctaaaag atttaggtat tcttcaatag tatagtttat tgaaagtggt gttggagtgc
    15661 atgaggctgc taacggtgta ttatctggaa ttacagcaac ggcaccattt ttaattgcat
    15721 ttattttacc taaaagagga tctttttgta aagcttcaag agttttatca tcaccataag
    15781 ttatgattac atcagcatca tttatcttat ttgcttcttc tgcagaaatt tcctttgcaa
    15841 aactatcttc actctcaaat tcttttaatg attcagggaa aactagacct aaatctgtta
    15901 aataatttgc tcttggatct ttgctagtat aaatccagaa ttttgatgta tctgcagcat
    15961 taatcatagt aaataatact ttttttcctt tgatttttcc tttgatttct ggatgttttt
    16021 ctaattcttt ggatatacga gcttcagtat ttttgattaa ctcatcacct tctttttcca
    16081 tacctaaggc ttttgaatca attttaatca tatctctcca taaagtttgc caaggtttag
    16141 atttgtatgc tgctacagga gcaatttttg atagagtgtc ataatcttct ttagttatac
    16201 cagaataacc tgctaagata acatctggtt tagaatttga tattgcttca aagttaagtc
    16261 catccaaatc gtcaaatagg ttagctttac catttagttc tttgattttt tcttctgtcc
    16321 atggtaaaac tcctttatca gcacttacac cgtaatttgc ttttgaaaat ccaacaggaa
    16381 ctattcctaa agctaatgct acatcatgat ttccccaagc aatagttgca actctttcag
    16441 gttttttatc taatatagtt tgaccgaaag catgatctaa aactatttta tcaggcgcat
    16501 gctctttaga agtattttct tcatttttaa cagaattaga agaacaagcg ctaaaaacta
    16561 tacacatagc taaaatagct attagaaaaa atttgttttt cataaaaact ccttaaacat
    16621 atttcaagtc tattgtatcg ataataatta tcattgtcaa tagatacagt tacaaattta
    16681 ataatataat tttttcaaaa aagg
//
LOCUS       DAVBCG010000034        16304 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBCG010000034 DAVBCG010000000
VERSION     DAVBCG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.034"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..323)
                     /locus_tag="VQ495_000922"
     CDS             complement(<1..323)
                     /locus_tag="VQ495_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2530897.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPI"
     gene            502..819
                     /locus_tag="VQ495_000923"
     CDS             502..819
                     /locus_tag="VQ495_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2530898.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            1058..2617
                     /locus_tag="VQ495_000924"
     CDS             1058..2617
                     /locus_tag="VQ495_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2530899.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(2661..3560)
                     /gene="htpX"
                     /locus_tag="VQ495_000925"
     CDS             complement(2661..3560)
                     /gene="htpX"
                     /locus_tag="VQ495_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2530900.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(3562..4122)
                     /locus_tag="VQ495_000926"
     CDS             complement(3562..4122)
                     /locus_tag="VQ495_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2530901.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            4216..4929
                     /gene="rsmG"
                     /locus_tag="VQ495_000927"
     CDS             4216..4929
                     /gene="rsmG"
                     /locus_tag="VQ495_000927"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2530902.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            5232..6515
                     /locus_tag="VQ495_000928"
     CDS             5232..6515
                     /locus_tag="VQ495_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2530903.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6710..8281)
                     /gene="ffh"
                     /locus_tag="VQ495_000929"
     CDS             complement(6710..8281)
                     /gene="ffh"
                     /locus_tag="VQ495_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2530904.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(8293..8625)
                     /locus_tag="VQ495_000930"
     CDS             complement(8293..8625)
                     /locus_tag="VQ495_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2530905.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(8716..9093)
                     /locus_tag="VQ495_000931"
     CDS             complement(8716..9093)
                     /locus_tag="VQ495_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2530906.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            9165..10469
                     /locus_tag="VQ495_000932"
     CDS             9165..10469
                     /locus_tag="VQ495_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2530907.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            10482..11288
                     /gene="yidA"
                     /locus_tag="VQ495_000933"
     CDS             10482..11288
                     /gene="yidA"
                     /locus_tag="VQ495_000933"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2530908.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            11331..12329
                     /locus_tag="VQ495_000934"
     CDS             11331..12329
                     /locus_tag="VQ495_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2530909.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(12496..12567)
                     /locus_tag="VQ495_000935"
     tRNA            complement(12496..12567)
                     /locus_tag="VQ495_000935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12532..12534),aa:Arg,seq:cct)
     gene            complement(12610..12957)
                     /gene="rplS"
                     /locus_tag="VQ495_000936"
     CDS             complement(12610..12957)
                     /gene="rplS"
                     /locus_tag="VQ495_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2530910.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(13076..13405)
                     /gene="crcB"
                     /locus_tag="VQ495_000937"
     CDS             complement(13076..13405)
                     /gene="crcB"
                     /locus_tag="VQ495_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2530911.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(13399..13773)
                     /gene="crcB"
                     /locus_tag="VQ495_000938"
     CDS             complement(13399..13773)
                     /gene="crcB"
                     /locus_tag="VQ495_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2530912.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            complement(13770..14036)
                     /locus_tag="VQ495_000939"
     CDS             complement(13770..14036)
                     /locus_tag="VQ495_000939"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2530913.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
     gene            complement(14152..14595)
                     /locus_tag="VQ495_000940"
     CDS             complement(14152..14595)
                     /locus_tag="VQ495_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2530914.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            14699..15634
                     /locus_tag="VQ495_000941"
     CDS             14699..15634
                     /locus_tag="VQ495_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2530915.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            15688..15972
                     /locus_tag="VQ495_000942"
     CDS             15688..15972
                     /locus_tag="VQ495_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2530916.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(16029..16247)
                     /locus_tag="VQ495_000943"
     CDS             complement(16029..16247)
                     /locus_tag="VQ495_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530917.1"
                     /translation="MSISPRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
BASE COUNT         5048 a         3573 c         2931 g         4752 t
ORIGIN      
        1 tcaatgggca agaccaagtc atcatctaaa acttcttgat ctagctggtt agttgcgcct
       61 tccatgaaaa cttccgtgac tggataagat tcaactaact caactggctc catactgcga
      121 ctcgaagcaa gaacaatggt ataagatagt tgataatcta agaaatacat acggtcttca
      181 tattgtactt tcccaactgc aaggatatct tttacatcta aaatttcttg attacgtgca
      241 cgcaggtcat caactaaatc taacgtttgt tcaaagttca aaccttcaga ctgcttacga
      301 atttcttgaa tatttaattt catacttcct ccataaagat ttactctctt gattatacca
      361 tgaaaaggct acaaatcagc acaccaaact ttgtaattaa aattcaaaat tttaacatat
      421 ttactatgac agttttattt tttagtgcta tactataggg aaagagtaca tcagatcaag
      481 gaggatgctc atatggaaga catggaagac aagaaactca ttcaactcct atccaagtta
      541 aataaaagct accaaaactg taaacagggt acggcagatg atattcgact acaagagctg
      601 ctaaacacta ctatgcaaga gctcaaaaaa acggaaaagt tgaacaacag tatcttaatt
      661 gatcttgaga aattttacca acccaccagt cctctgattg gactgggtag cctaaaacta
      721 aacgatcaag cacgcactgc ttggcgaaac tatgataaaa tccattacga gcatgtcaaa
      781 cacgtactaa gtctctatgg acctgttttt ggattttaga gcatagaatt tccagttttc
      841 tgttgacaaa atttccttaa aagtatagta taaagatact aatactcgga ggtaagggag
      901 acatgaacaa ctaagtctat caaataaaga acctttattt agtagatctt gtttttgtct
      961 ctttttgtgt gctcttttat gctctttttc tggcatgtta atagagtttt tttgacatag
     1021 actttgggct ctactaggta aagtagagct ttttgttatg cactatcgac attctagaaa
     1081 gggcaatagt atgataaaaa tcaatcacct gaccatcaca caaaacaaag atttacgaga
     1141 ccttgtctct gacctaagca tgaccataca agacggggaa aaggtagcta ttattggcga
     1201 agaaggaaat ggtaaatcaa ccttacttaa aactttaatg ggggaagatt tgtctgattt
     1261 cactatcaag ggagacatcc agtctgatta tcagtcactg gcctacattc ctcaaaaagt
     1321 ccctgaggac ctaaaaaaga aaactttaca cgactacttc tttttagatt ctattgattt
     1381 agactacagt atcctctatc gtttggcgga ggaattgcat tttgatagca atcgtttcgc
     1441 aagtgaccaa gagattggca atctatcagg gggcgaagct ttgaaaattc agcttatcca
     1501 tgagttagcc aaaccctttg agattctatt tttagatgaa ccttcaaatg acctagacct
     1561 tgagacagtt gattggctaa aaggccagat tcaaaagacc aggcaaaccg ttattttcat
     1621 ttcccatgat gaagactttc tttctgaaac ggcagacact attgttcact tgcgactggt
     1681 caaacaccgt aaagaagcgg aaacgctagt agagcattta gactatgata gctatagtga
     1741 gcagagaaag gctaattttg ccaaacaaag tcagcaagct gctaacaacc aaagagccta
     1801 cgataaaacc atggaaaaac atcggagagt taagcaaaat gtagaaactg cgcttcgagc
     1861 taccaaagat agtactgccg gtcgcctatt ggctaaaaag atgaaaactg tcctctcaca
     1921 agaaaaacgc tacgaaaaga cagctcagtc catgactcaa aagccacttg aagaggaaca
     1981 aatccaactt ttcttttcag acatccaacc attaccagct tctaaagtct tagtccaact
     2041 ggaaaaagaa aatttgtcca ttgacgaccg tgttttggct caggaactgc aactaactgt
     2101 ccgtggccaa gaaaaaatcg gtatcatcgg cccaaatggt gttgggaaat caactctgtt
     2161 agccaaatta cagcaacttc tgaatgataa aagagagatt tcacttggtt ttatgccaca
     2221 agattaccac aaaaaactgc aattggattt atcctcaata gcttatatca gcaaaactgg
     2281 ggaaaaagag gaacttcaga aaatccaatc tcacctagcc agtctcaatt tcagttatcc
     2341 agaaatgcag catcaaattc gctccttatc tggcggacaa cagggaaaac tcctgctttt
     2401 ggatctagtt ctgcgcaaac caaactttct cctgctggat gaacccacac gaaacttttc
     2461 tcccacttct caaccccaaa tcagaaaact ctttgcaacc tatccaggcg gtctcatcac
     2521 tgtttcgcat gaccgtcgtt tcttaaaaga agtctgctcg atcatctatc gcatgacaga
     2581 acacggtttg aagctagtta atttagaaga tttataaatt tgcaacatag caaaaatcca
     2641 gagacgacct ctggattctt ttacatctgt tttaaacgtt caatccgttc tgagataggt
     2701 gggtgggtat aaaagagttt ttggaacccc ccacctttct taggatcatt gatataaagg
     2761 gcactgctag catcatcgac gtggcgactc ataggtttgc tattgtccaa cttatctagg
     2821 gcattaatca ttccctgggg attgcgagtc agctcgacac tagatgcatc tgccagaaat
     2881 tccctctgac gagaaatagc gagctgaacc aaggttgcag cgagaggtgc cagtacaata
     2941 gctagtaggg aaaccactag cataatgatt tcaagaccat ttccatctcg gtcatcatca
     3001 cttcgtctgc gacctgctcc accccaccac atcatacgac ctgccatact agaaagcatg
     3061 gtgatagcac tagcaagggc aactgcaata gtcgaaatac ggatatcata attacgaata
     3121 tgactgactt catgtcccat aacagcttct agttcttcac gattcatgat agctagtaga
     3181 cctgaagtcg cagcaacagc cgcattttga ggattagaac ctgtcgcaaa ggcatttaag
     3241 gctggatcat caatgatgaa aacacggggc ataggaatct gagcgaccag agccatatct
     3301 tccactacat ggtagaggtc tggtgccgtt tgctcatcca cctcacgcgc tccattcatg
     3361 gacatgacaa tctctgtcga ttgaaaaatc atagacaaag cgtagataaa gccgataatc
     3421 agtgcgataa ccaaaccacc aagtccagat cttataaaga gataaccaac cgcataacca
     3481 acaagagcta agagtaggaa aaataccagc aacaaaatcc aggtttttcg tttattgctt
     3541 gcaatttgat caaacaacat cttagtcacc taaaccgcta aaatcaactt taggaaccga
     3601 cttttcctct tcagatgttt gaaggaaatc tgccgcttta aatccaaaca ttccagcgat
     3661 aatattgctc gggaaagttt ctaattttac attgtagttg ctgacaacac tgttatagag
     3721 ttgacgagag taagaaattt tattttctgt gtttgtcaac tcctcttgca atttaacaaa
     3781 gttagcacta gctttcaaat ctggatagct ttctgcaact gcaaaaatac ctgaaacctg
     3841 acgagtgagg gcatcactgg ctttcatagc ttctgctggt gaagtcgctg ccgccacttg
     3901 gttacgtagt tctgccacct tttcaagggt agaaccttca tatttggcat aaccttttac
     3961 agtctcaatc aagtttggca agaggtcatt tcgacgtttc aactgaacat caatctgact
     4021 ccaagcctcc ttggtttgca tacgattttt aaccaaaccg ttatagctaa caatcacaaa
     4081 aataacaata agagcgataa ctccaagaat aatccaagtc ataatataag tcctttctgc
     4141 ttttagatta gtaccagtat atcaaatttt ctatgattgt ggtaaaataa gatgatacta
     4201 aagaaggaaa taactatgaa accaaaaaca ttttacaact tgcttgccga gcagaatctt
     4261 ccactttcgg accagcaaaa agaacaattt gaacgttatt ttgagctctt ggtcgagtgg
     4321 aatgagaaga ttaatttgac ggcgattacg gacaaggaag aagtttatct caaacatttt
     4381 tacgattcga ttgcacccat tcttcaaggt ttgattccca atgaaactat caaacttctt
     4441 gatatcgggg ctggggcagg atttcctagt ctaccaatga aaattctcta tccggagtta
     4501 gatgtgacca ttattgattc actcaataag cgcatcaact tcctacaact cttggctcaa
     4561 gaactggatt tgaacggagt tcatttctac cacggacgtg ccgaagattt tgcccaagac
     4621 aagaacttcc gtgctcaata tgattttgta acagctcgtg cggttgcccg tatgcaggtc
     4681 ctatctgaat tgaccattcc ctaccttaag gttggtggaa aactattggc actcaaggcc
     4741 agcaatgcgc ctgaggaatt attagaagct aagaatgccc tcaatctcct ttttagtaag
     4801 gtcgaagaca atctcagcta cgccctaccg aatagagatc cgcgctatat cacagtggta
     4861 gaaaagaaaa aagaaacacc aaataaatat ccgcgtaagg ctggtatgcc aaataaacgc
     4921 ccactttaaa ttttttagta aacaaatgtt tacaaaatca gcctcgctct tttatttcta
     4981 ggctcgggaa aaaatgattt acaaaatcag cctcgctctt ttatttctag gctcgggaaa
     5041 aaatgattta caaaatcatt tttttctgct atactatcct aagcaaaggt ttttaatgtc
     5101 atcccgtgag gtgacgaaga cgcagaaata tttaaaactc tttaaaatct aaattttaaa
     5161 gaagtcttac tctgagggcc tattgctgta aaataatggg ctcttttttg atgcccaaaa
     5221 gtgaggttta tatgaaacaa gaatcaactg ttgatttgtt actagacgtt gaccaacgtc
     5281 cttcggctgg aaaaggaatt ctccttagtt tccaacacgt tttcgccatg tttggtgcga
     5341 ccatcttggt accattgatt ttgggaatgc ctgtatctgt tgcccttttt gcttcaggtg
     5401 ttggaacact catctacatg attgctactg gttttaaagt tccagtttat ctaggttctt
     5461 catttgcctt tatcacagct atgtcactgg ctatgaaaga aatggggggg gatgtatctg
     5521 ctgcccaaac aggggttatc ttgactggtt tggtctatgt ccttgttgct accagcatcc
     5581 gatttgtagg aacaaaatgg attgataaac tcttgccacc aatcattatc ggtcctatga
     5641 tcatcgttat cggtcttgga cttgcaggtt cagctgttac caatgcaggt cttgtagcag
     5701 acggaaattg gaaaaatgct ctggtagccg ttgttacttt cctaattgct gcctttatca
     5761 atacaaaagg aaaaggcttc ctacgaatca ttccattcct ctttgccatt atcggtggtt
     5821 accttttcgc actaactctt ggcttggttg actttacacc agttcttaaa gccaactggt
     5881 tcgaaattcc tggtttctac ttgccattta gcacaggtgg tgcctttaaa gagtacaatc
     5941 tttactttgg tccagaagcc atcgctatct tgccaatcgc tatcgtaaca atttctgaac
     6001 atatcggaga ccatactgtt ttgggtcaaa tctgtggtcg tcaattctta aaagaaccag
     6061 gtcttcaccg tactcttctt ggtgacggta tcgcaacttc tgtttctgcc ttccttggtg
     6121 gaccagccaa tacaacttac ggagaaaata caggggttat cggtatgact cgtatcgctt
     6181 ctgtctcagt tatccgtaac gctgccttca tcgcgattgc cctcagcttc cttggtaaat
     6241 tcactgcctt gatttcaact attccaaacg ctgtacttgg tggtatgtca atccttctct
     6301 atggggttat cgccagcaat ggtttgaaag tcttgattaa agaacgtgtt gatttcgctc
     6361 aaatgcgaaa cctcatcatc gcaagtgcta tgttggttct tggacttgga ggagctatcc
     6421 ttaaacttgg tccagttaca ctttcaggta ctgccctttc agccatgaca ggaatcatct
     6481 tgaacttgat cttgccatac gaaaataaag actaagagtc taaatacacc taatccactc
     6541 agacagctga gtggattttt cgtataccat aataaaagtg tcttaacaaa attattaaaa
     6601 tcaaaaaacg tataatatca gatattctaa aaccttgata ctgtacgttt tatcatagaa
     6661 atttttactt tattttctca tcaaatgaga tttgcatcaa tctcttgtct tacttgcgtt
     6721 tcttcttcgc tttcttcatt ttgttagcca tacgtttcat ggactgtttc atggcaaatt
     6781 caccaatttt acctttcaaa ccgccaccaa acatctggct catatctggc attcctgctc
     6841 ctccgagagc tgataagtca ggcataccgc cttgtcccat cattccttca agggcagaca
     6901 tatccattcc tcccatattt ggcatatttt taggaaggtt atttggatta atccccattt
     6961 gcttcatcat tttattcata tccccagaca taacaccctg catgagctgt ttagcctggt
     7021 taaagtcctt gatgaattta ttgacttcga cgaatgtatt tccagaacca gcagcaatac
     7081 gacggcgacg gcttggattt aacaaatctg ggttttcacg ctcttcaggt gtcatcgaag
     7141 acacaatggc acgtttacga gcaatctggc gttcatccac cttcatgttt tgaagggctg
     7201 gattgttggc catacctgga atcatcttga gcaagtcttc catcggcccc atattttgca
     7261 cctgatctaa ttgatcgatg aaatcattaa aatcaaaggt gttttcgcgc atcttctcag
     7321 ccatttcaag ggctttttgt tcatcgtatt cctgagaagc tttctcaatc aaagtgagca
     7381 tatcccccat accaagtata cggctagaca tgcggtctgg gtggaaggtt tcaatgtccg
     7441 taatcttttc acctgtacca gtgaacttga ttggttttcc agtaatgtga cgaacagaca
     7501 gagcagcacc accacgagta tcgccatcaa tcttggtaag gatgacccca gtcacttcca
     7561 actgagcatt aaactcacgc gcaacattgg ctgcttcctg accaatcata gcatcaacga
     7621 caagcaagat ttcatttggt tgagccaatg ctttcacatc acgaagctca ttcatgagga
     7681 gctcatcaat ctgcaaacga cccgcagtat caatcaagac atagtcgtta tgattagttt
     7741 gggcttgctc caaaccttga cgtacaatct caacagctgg tacttctgtt ccaagtgcaa
     7801 agacaggcac atcaatctgt tgtcccaagg tcttaagctg gtcaatggca gctggacgat
     7861 aaatatccgc cgcaatcatc aaaggacgag cattttcttc tttcttgagt ttgttggcca
     7921 atttaccagc aaaggttgtt ttaccagccc cttgtaaacc aaccatcatg atgatggttg
     7981 gaatcttagg tgacttgata atttctgccg tatcagaacc taaaacggct gtcagttcct
     8041 catcaacgat tttaataatc tgttgcgcag gattaagtgt atcaatgacc tcatgcccga
     8101 ctgcacgctc acgaactttc ttgataaagt cctttacaac aggcaaggca acgtcggcct
     8161 cgagcaaggc caagcgaatt tctttggttg cctcttggac atcagattca gagatttttc
     8221 cttttttacg tagattttta aagacgttct gcaaacgttc tgttaaactt tcaaatgcca
     8281 tttttcttcc tcttattctc tattatcaat gcttgttaaa atttctatct gctcctgcag
     8341 aaagtcatcc ttgggatagc gctccaaaat ctgatcaaaa atctgactgc ggacaatata
     8401 gtccgagtac atgtgcaatt tcatctcata atcttccaga atcttttctg ttcgcttgat
     8461 attgtcatag acagcctgac gactgacacc aaactcctcg gcaatttcag caaggctgta
     8521 atcatcagcg tagtagagct cgatataatt catttgctta tctgtcaaaa gcgccgcata
     8581 aaattcaaag agcgcattca tacgattggt tttttcgatt tccataactt ttattatacc
     8641 aaaaattagc ctaatctacc acactaggaa gccgatccaa gaagatagat agctaaattt
     8701 gaaaaagaca tgagcctagc cccaagtaat ttccaattga tagctggcaa aaggatgtcc
     8761 ctcttgattt tgtagttgat aatctagttc aatcttttgc ctatcaactt gataatggct
     8821 cgtttggatg ataaactcct gcatgcccat aggtgtagga atataggcta aactatcgct
     8881 atcctttaga aagcgcataa tggtcttggg attagaaaat cggctcatca taagttcttg
     8941 accatgaaat ttaatcacta ctttttcctt ttcctcatta tagaaaagca ggtagctata
     9001 atctcctttt tcatgcactt ccacatcata aagctggtca atcacttcca actgctcatc
     9061 aaactgaatt gtatttcgca tccgaatctt cacatcaggc cctctttctt gtctcttgtc
     9121 ctactatttt accaaaaaga gcaggatttt gctataatgg tcatatgaac gaaaaagtat
     9181 tccgtgaccc agttcacaac tacatccatg tcaataatca aatcatctat gacttgatta
     9241 atacaaaaga atttcagcgt ttgcgccgga tcaaacaact gggaacttcc agttatacct
     9301 tccacggtgg agaacacagt cgcttctctc actgtctagg agtctatgaa attgcacgac
     9361 gcatcacaga gattttcgaa gaaaaatatc ctgaggaatg gaatcctgcc gagtctctct
     9421 tgaccgtgac cgctgctctc ctacacgacc ttgggcatgg tgcctactcc catacttttg
     9481 aaaatctctt tgatacagac catgaagcca ttactcagga gattattcaa aatcctgaga
     9541 cagagattca ccaagtcctg ctacaagtgg cacctgattt cccagaaaag gtggccagtg
     9601 tcattgacca tacctatcct aataagcagg tcgtgcagct catttctagt cagattgacg
     9661 cagatcgcat ggactatctc ttgcgcgact cctattttac aggagcatcc tatggggaat
     9721 ttgacctgac tcgcatcctc cgagtcattc gtcctgtcga aaatggtatc gcctttcagc
     9781 gcaatggcat gcacgccatc gaagactacg tcctcagtcg ctaccagatg tacatgcagg
     9841 tttatttcca cccagcaaca cgcgccatgg aagttctcct acagaatctt ctcaagcgcg
     9901 ccaaggaact ctaccctgag gacaagaatt tctttgcacg aacttcccca catctcctgc
     9961 ctttcttcga aaaaaatgtg accttgactg actatctggc tctggatgat ggcgtgatga
    10021 atacctactt ccagctttgg atgactagtc ctgacaaaat tcttgcagat ttatctcaac
    10081 gctttgtcaa ccgcaaggtc tttaaatcca ttaccttttc acaagaggac caagatcaac
    10141 ttactagcat gagaaaattg gttgaggaca tcggctttga tcccgactac tatactgcca
    10201 ttcataagaa ctttgacctc ccttatgata tctatcgtcc cgaatctcaa aatccacgga
    10261 cacagattga gattttacaa aaaaatggag aactggccga actctctagc ctgtctccta
    10321 tcgtccaatc ccttgctggc agtcgccacg gagataatcg tttctatttc cctaaagaaa
    10381 tgttagacca aaacagcatc ttcgcaagca ttacccagca atttttacac ttgattgaga
    10441 acgatcattt taccccaaat aaaaactaga agaggaattt tatgagtatt aaactaattg
    10501 ccgtcgatat tgacggaacc ctagtcaaca gccaaaagga aatcactcct gaagtctttt
    10561 ctgccatcca agatgccaaa gaagctggtg tcaaagtcgt gattgcaact ggccgcccca
    10621 tcgcaggtgt tgccaaactt ctggacgact tgcagttgag agaccaaggt gactatgtgg
    10681 tgaccttcaa tggtgccctt gtccaagaaa ctgctactgg ccatgagatt atcagcgaat
    10741 ccttgaccta tgaggattat ctggatatgg aattcctcag tcgcaagctc ggtgtccaca
    10801 tgcatgccat taccaaggac ggtatctata ctgcaaatcg caatatcgga aaatacactg
    10861 tacacgaatc aaccctcgtc agcatgccta tcttctaccg tacccctgaa gaaatggctg
    10921 gcaaagaaat tgttaaatgt atgtttatcg atgaaccaga aattctcgat gctgcgattg
    10981 aaaaaattcc agcagaattt tacgagcgct actccatcaa caaatctgct cctttctacc
    11041 tcgaactcct taaaaagaat gtagacaagg gttcagccat tactcacttg gctgaaaaac
    11101 tcggattgac caaagatgaa accatggcaa tcggtgatga agaaaatgac cgtcccatgc
    11161 tggaagtcgt tggaaacccc gttgtcatgg aaaatggaaa tccagaaatc aaaaaaatcg
    11221 ccaaatacat caccaaaaca aatgacgaat ccggcgttgc ccatgccatc cgaacatggg
    11281 tactgtaaaa gtatcatttt tcaataagaa ttgattagca ataaaatcca atgaattttt
    11341 ttagcaaact atttaattta aaacaaaata atcataatag agacacaaat tctgattgta
    11401 acaattttta cctaaacgaa ttagaatgtg gccttactcc tgggcaactc atactcatag
    11461 attggactca aaaaacaggg agaaattata atttcccaag atattttaaa tactctcttc
    11521 aaattgaccc tgaatctaca cacaatcaat tatacaaatt aggatacttc actaaaaata
    11581 agactttatc atatcttaca gtagtagaat taaaaactat attatctaaa cataatttag
    11641 ctacttctgg aaaaaaagca gaattaatta caagaataat taataatgtt aacattgaca
    11701 atttagatat tccgttcgaa tttaaactaa caaaagaagc acaaaatctt attatcgaac
    11761 atagtgacta tatcaaagca tactatgata aagacataag tatggaagat tattgtaaag
    11821 aaaaaaacaa tatctctttt aaagcaactt ttggtgatat aaaatggagt ctcttaaata
    11881 aacaagctca taggaatact gtatcaggag attttggatg cttatctaac acacgaaagg
    11941 ctcagggaag acatttggaa caagaaggta atattaaaca tgctttaata tattacatag
    12001 aatctttgat aattactatt tcaggattag aaaacaattt ttcagccact gattatccag
    12061 tatattatcc cgattcgata cctgactact cactaaaaca tattcaaaca ttaatggaat
    12121 cattatctga tgacgattat gattttgctt ttgatgaagc attatttcgc ttctcaattt
    12181 tgaatgcaaa tcatttttta tccaaggaag atattgacta tttaagagtt aatttacctc
    12241 gttccactgc tgaagaaata aacaattact taaagaaata tgaatgttat agtcctttaa
    12301 ataatttaga acttgacgat tttgaataaa ttgactatac aaacatttat atactcgata
    12361 tagtctcaat tttatctgat gattgcccaa atttttcaat aataaaacgc ataatattat
    12421 ggagacaatc ccctatatta tgcgttcttt taatatcaaa gactctttga caaacttctt
    12481 tgatatctaa ttacatgccc cctgcaggaa tcgaacctgc aactactcct taggagggag
    12541 ttgttatatc cattgaacta agggagctag ataaaaactc tgctgaaaaa gcagagtttt
    12601 ttagtcgaat taacgacgga tttctttgat acgagctgcc ttaccttgaa gagcacgcaa
    12661 gtagtacaat ttcgcacgac gtactttacc gtaacgtaca acttcgattt tttcaacacg
    12721 tggagtgtgg attgggaaga tacgctcaac acctacaccg ttagagattt tacgaactgt
    12781 gtagttttca gagatgccag cacctttacg tgcaataaca acaccttcaa aaatctggat
    12841 acgttcacgg ttaccttcga caactttcgc atgtacacga acagtgtcac caggacggaa
    12901 tgatgggata tctgtacgaa gttgaccttc agtcaagctt tggattaatg gattcatttt
    12961 attctcctat ctttgtcaat cttgaggaat cttcctcagc ggataaactg tatttttgtg
    13021 cgtccattac acacaagata tagtttacca aatttctaaa aaaaagtaaa gaaatttata
    13081 gcaaaattcc taaaaaaatc gcaaccaaac cacctatgta agtcaaagtc aggtaagaat
    13141 aaaagacctt cttatcactt agcagtcttt gaagttcgtc attcaaggtc gaaaaagttg
    13201 ttaaaccgcc acaaaatcct gttgctagaa tagcatatac ttccttagac tctacatgat
    13261 tgtagaatac tccaattaaa aaacaaccta aaagattagc tatgagcgtt ccgagtggca
    13321 atttagaagc ttgattatag cgagaaaaga aataccgtac gagtgctccc aagccacagg
    13381 cgattgcaag atagacgatt accatttctt cctccccaaa tagtaagcta aaagcaggcc
    13441 tccaccgata ctcaaaagca aatacaggac taaactaaaa taacgccctg tatcaagcag
    13501 cttcacagta tcaagcatta ggctggaaaa agttgttaaa cctccgcaaa aacccgtccc
    13561 cagtgctaaa atcaagccct tactagtccc cttatagacc agatagccct tgacaagaaa
    13621 aatcaagcag aaaattccca gatagttgac aaagagagtg ccccaaggaa agtctggact
    13681 ggctggtaac caggtggaaa ctagatatcg gacaagtccg cccaacatag cagctagaaa
    13741 aattcctagc ggataaaatt gttctttttt catttgatat tttgatcctg ataatcacgc
    13801 gaacgtttga gtatgtacga aaaagtcgcg acaatagtct cctgatagcg cttgtcctct
    13861 acacgactcc taaccttttc aaaaataact tcttctctct tagtatctaa aatcggttta
    13921 cctgaagctt tcttataagc aaccaccccc tcaactaaat gcattcgttc ttctaacagt
    13981 ttgacaattt ggtcgtcgat ttgatcgatt tcttgacgaa taatatctaa atccatacag
    14041 tctcctcctt tatttgaatt attgtatcaa aaagccatca attaggctag taaaatccca
    14101 actctcgata agaaaaatgc actgattgat tacatcagcg cacttttatg cttatcctac
    14161 tttagcagcc aattcttctg cgaattgttc caagcgttca atatcttctt cctcggcaga
    14221 aagatcaact ttaacacact ctgaaccttt ttctgctcct gttgacacaa agacacggtc
    14281 aaagtcatca acagccttac agaattcatc gtagaaggta tctcctgagc cgaccactcc
    14341 gtagattttg ccattcaagt tgagatctgc taggtcttcg tagaagtcca tcatctcatc
    14401 tggcaattca ccatcaccat aagtataggt cgcaacgata gcgatgtctg cttccaagaa
    14461 gtctgaagcg tcaacagttg tacattcatc aacatcaaca tccaagccca agtcacgtaa
    14521 tttgtctgct acaatatctg caatttcttc ggtattaccg gtcatactgg caaatacaat
    14581 ttttgctaat gccatatcgt cctcctcaat ttatctttct ccattatatc acatcttttt
    14641 cattttaaaa ataaaaattc ttgtgctaca atgaaatgag aaagaattga ggttatttat
    14701 ggagatttgc caacaaattt tagaaaaaat taaagaatac aacaccatta tcattcatcg
    14761 tcatatgaaa ccagaccctg atgccttggg aagtcaggtg ggattgaaag ccttgctgga
    14821 acatcatttc ccagaaaaaa ccatcaaagc cgtcggtttt gatgaaccaa ctcttacttg
    14881 gatggctgag atggatcttg ttgaagatag agcctaccaa ggcgcacttg tcatcgtctg
    14941 tgatacagct aatactgctc gtatcgatga taagcgctat agtcaaggtg attttctcat
    15001 taagattgac caccatccaa atgatgatgt atacggtgac ctgtcttggg tcgatactag
    15061 ttcaagtagc gctagcgaga tgattaccct atttgcccaa acaacccaac tagccttggc
    15121 agatcgcgat gctgagttgc tctttgcagg aattgtcggt gatacaggtc gcttcctcta
    15181 cccttctacc actgcacgga ctcttcgcct ggctgcttat ttgagagaac ataactttga
    15241 ctttgcggct ctcactcgca aaatggacac tatgagctac aaaattgcta aactgcaagg
    15301 ctacatctac gaccatctgg aagtggatga aaatggtgct gctcgcgtta tcctgagtca
    15361 gaaaatcttg aaacaataca atgtaaccga tgctgaaact gcggccattg taggtgcacc
    15421 tgggcgtatt gatagtgtta gtctctgggg aatttttgtg gaacaggctg atggccacta
    15481 ccgcgttcgc ttacgcagta aaatccatcc tatcaatgaa atcgccaagg agcatgatgg
    15541 tggaggccac cctctagcaa gtggtgctaa ttcctatagc ctagaagaaa atgaaatcat
    15601 ctaccaaaag ttaaaaaact tgcttaaaaa ctgataaaat acttgccaaa cttttcagaa
    15661 tctgatagac tagtatggta acaatctatg gctcgcaaag agaccatggc agaaaggaaa
    15721 tattgcaaaa tgaaaaaaga tatccatcca gaatatcgcc cagttgtctt catggacaca
    15781 actactggtt accaattcct tagcggttca acaaaacgct ctaacgaaac agttgagttc
    15841 gaaggcgaaa cttacccatt gatccgtgtg gaaatttcat cagactcaca cccattctac
    15901 actggacgtc aaaagttcac tcaagcagat ggacgcgtgg atcgtttcaa caaaaaatac
    15961 ggtctcaaat aatgataaaa gaacagtttc gactgttctt ttttgtttct tgaaatcaac
    16021 tgctgttttc atgttccaga ctcatctgta ggttcggttt ccatgctact aggcaggaag
    16081 gaaatagctg tttcaacacg tccataatga gctgtactat tgtcacgaac cacactttca
    16141 ttgatggtcc aagtggaatt cattttctta aaagcttctc ggactttttc caaatctttg
    16201 gaggcaatgg cctgttccaa ggcttcaaaa cgaggactta tactcatctg ctttcaaaaa
    16261 gcattctagt ccatctccga ttaacgatgg actttatcac ctcc
//
LOCUS       DAVBCG010000035        16169 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBCG010000035 DAVBCG010000000
VERSION     DAVBCG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.035"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(192..1040)
                     /gene="dprA"
                     /locus_tag="VQ495_000944"
     CDS             complement(192..1040)
                     /gene="dprA"
                     /locus_tag="VQ495_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2530918.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1138..1827)
                     /locus_tag="VQ495_000945"
     CDS             complement(1138..1827)
                     /locus_tag="VQ495_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2530919.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            complement(1839..2717)
                     /locus_tag="VQ495_000946"
     CDS             complement(1839..2717)
                     /locus_tag="VQ495_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2530920.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2707..3576)
                     /gene="licA"
                     /locus_tag="VQ495_000947"
     CDS             complement(2707..3576)
                     /gene="licA"
                     /locus_tag="VQ495_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2530921.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3593..4615)
                     /locus_tag="VQ495_000948"
     CDS             complement(3593..4615)
                     /locus_tag="VQ495_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2530922.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4620..5327)
                     /locus_tag="VQ495_000949"
     CDS             complement(4620..5327)
                     /locus_tag="VQ495_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2530923.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5663..7150
                     /gene="tacF"
                     /locus_tag="VQ495_000950"
     CDS             5663..7150
                     /gene="tacF"
                     /locus_tag="VQ495_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2530924.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7160..7963
                     /locus_tag="VQ495_000951"
     CDS             7160..7963
                     /locus_tag="VQ495_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2530925.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            7965..8774
                     /locus_tag="VQ495_000952"
     CDS             7965..8774
                     /locus_tag="VQ495_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2530926.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9049..12225)
                     /gene="carB"
                     /locus_tag="VQ495_000953"
     CDS             complement(9049..12225)
                     /gene="carB"
                     /locus_tag="VQ495_000953"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2530927.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(12537..13616)
                     /gene="carA"
                     /locus_tag="VQ495_000954"
     CDS             complement(12537..13616)
                     /gene="carA"
                     /locus_tag="VQ495_000954"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2530928.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(13666..14589)
                     /locus_tag="VQ495_000955"
     CDS             complement(13666..14589)
                     /locus_tag="VQ495_000955"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2530929.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(14608..15129)
                     /gene="pyrR"
                     /locus_tag="VQ495_000956"
     CDS             complement(14608..15129)
                     /gene="pyrR"
                     /locus_tag="VQ495_000956"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2530930.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(15340..15969)
                     /gene="nth"
                     /locus_tag="VQ495_000957"
     CDS             complement(15340..15969)
                     /gene="nth"
                     /locus_tag="VQ495_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2530931.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(15969..>16169)
                     /locus_tag="VQ495_000958"
     CDS             complement(15969..>16169)
                     /locus_tag="VQ495_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2530932.1"
                     /translation="AESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQ
                     QAVKKEENSPFAGLQGLFDGDE"
BASE COUNT         4804 a         3431 c         2993 g         4941 t
ORIGIN      
        1 gaagctgacg tagtttgaag agattttcga agagtattat atgagaaaaa tcctctactc
       61 tactaatcca cttagaaaaa acgcatatta tcaagtgtta aaaacttgct aatatgcgtt
      121 ttattatgga aatgttgggt tcattttctc ttaaaattga ttttttctta gaattctagc
      181 ttaggtcatt tttaaaattc aaattccgca agaacatctt gcccactggt gaccaatttt
      241 gctccttctt gaatcaaatg atggcaaccg tctgatagtc catctaaaat gctaccagga
      301 atagcaaaga catcgcgtcc ttcttccatt gctcgctcac acgtaatgag actacctgaa
      361 cgcatcttag cctctgctac aatcacacca cgacaaagtc cagcaatgat gcgattacgg
      421 gcaggaaaat gaaatttcag aggttgttcg ccaggtccat attcacttag aaccagatgg
      481 tcattgccga tgtagtcttg caagcgttta ttggctttag gataaaacac atccagtcct
      541 gttccaatca ctgcaatggt ttttccgcca ttctgaagag ctgccatatg agctgctgtg
      601 tcaatgccct tggccagacc actgacaata accagttcat tttccaagcc ttgaatgact
      661 ttttcaactg atttagctcc ctgtttgcta caagcacgac tgcccacgac cgctaccttc
      721 gggaatttca ggaggtcaag atttcccttg taaaataaaa gtacaggcgc atcatatatt
      781 tcactcaaat cccaaggata acagtcatct aaaatagaga aagatggaaa tttttgaaac
      841 tctttcgaca aatgcgcatc gtctatctga aaataacgtt ccataaaaac ggctggatta
      901 cggcaacctg atatatctgc aatatcaccc aacaaaagct cctgatcaac attttcaccg
      961 tattctagaa ctttcaaaat ctgttgattg gtcaaacctg atttttttaa cttatagatt
     1021 tcatagtttg tgattttcat aaataactcc atttcttttt ctactcatct atctattcgt
     1081 aaaagttcgt aaaaaatcaa aaaaacatcc gactattttg tcagatgttg gaagctttta
     1141 attttcgttt ttaagaattt cttctaattt atgatagtct tggacactat caatctcata
     1201 aatgctattg ccttctaatt cttcaacata gacatctagc tctttgatgt tatccttaac
     1261 catattgtcc caatagagat caacaaattc gccgcttgca taagccttat cgataaagct
     1321 aacaattttt tctgctgttg gagcatccca gaaggataca ccactaagga tgcgacctgc
     1381 cttgctatca acaataatgt tttgaacctt gtagtcatct ccatagacca agaaccattc
     1441 gttggtacaa tcttcacgat agacactaaa ataagtagaa cgtgtcaaat cattgcggaa
     1501 catattttta aagagatagt tatcggcatc gataacataa ctattggcta attcttcttt
     1561 tacaagatag agagagtaaa agttattgta gtcagcgtat ttatcattga aaacgagacg
     1621 aacaccgtat ttctctttca agtaatcgaa ttgttcttta agataaccaa caatgatgat
     1681 gatgtcattg attccttttt ctttgagaaa ctcaatttgg tactcaatca aaggtttttg
     1741 attaacctga accaaggctt taggggtatt ttcagtcata ggacgcaagc gagttcccaa
     1801 tcccgctgct aagatgatgg ctttcacacg aatctccttt attctttaat aataatataa
     1861 actccagcaa tgacgacaag tgacgtcata atggtcagca tatctagcgg tgcacccaag
     1921 aaaacaactg caaacaagac cgtccatact acatagctca cgttcaagcc tgtagccttg
     1981 gctggttgca agcgattgat agcgatataa taagccaagt aggaaatcat atcaaaggct
     2041 gcaaaaacaa tcatgagacc tagcaattgt ccattggcta ctgcagtaaa tgactgatga
     2101 gagaagagca caatcacaag ataggacaag aacgaagtta cttgacggat taagagggct
     2161 tcgatttcac tcaattcact ttccatggca aaagagctaa gaacactctc acttccccat
     2221 gcaatagcac aaaccaaagc acaaagaatc ccaatgtaga aagaattaac ctgttcaact
     2281 ttataggtct gggcaataat ccctccaata atcaagacaa tcccaaatac agtatttttc
     2341 gaaatcttgt gcttcaaaaa gaagaaagcc aatagaactg aaatcgcagg gtaaatagcc
     2401 gatacagatg aagctaaaga acttccgata tacttaactg cataaagatt ggcctgcata
     2461 ccgatagggc ctgctagcaa ggctccgatg ataacactga cattgcgaat atttaagaaa
     2521 attgagaggc gaactttccc ttcttttacc aagagaaaag ctagtaagat aaagatgctc
     2581 aaaaaatcat gagctgcagc caccacaaag ggtgacaaat ctgtaaaaat cgaaaagata
     2641 taagcactaa ccgttagacc caagccccag aaaatacctg agagaaggcc aaaaggaact
     2701 ccgtttttac ttttcatctg aacctccata agaagccaaa cctttaacag ctctttggta
     2761 acgattcaca ccatagtcac caaaatcttc accttgctct tccttataga cagtccatag
     2821 actccaaata gtatcttgta aaattttata aatagcaatc ttttcatgag aaaccggtgt
     2881 ttggtcactc tcatagtgag ataagaaagt ttcttcctct tgggaagtga attcagactc
     2941 taaaaagagg gcagccaaat cccacattgg atcattcatt gatgaatatt cccagtcaat
     3001 caaataaagt cgtccttgag gtgattcgat aaagttttca ggcaccaaat cgatatgaca
     3061 agattttctg tcaacaccta agtcagccag tcttttctct aaggagaaga ctgcatttct
     3121 aacagattca tagttggcat aaggaatttg ttcttcaatc aaggattcgt attttttgat
     3181 ttcttcaaaa ggagcaaatt ctcctcttaa ttccttagca gacgtatgaa tagtttgtaa
     3241 tattggagca attttgtcga acttggtctt gattgacgtt gaatcaagcg taatcgcaga
     3301 ttcgatatac tcatttactt tgataccagc ttcaatatca aaaagataat tttttacatc
     3361 taagcctaaa tcctttagta gttcaagatt gtacttttca tcttgtcgat tgataagctt
     3421 ttctgtccct ttaccaaaga atttaacaat gtattgctta tttgttgttt tggccaaata
     3481 gttttgattg gtcattccac ccagttgttc aacactgagg acttcctctt cttgactaag
     3541 taaggaagaa attttttctt taatgatttt ctccacaatt aacctccagt acttatactt
     3601 cccacttaaa cactgtttta aaggctgtgt ttaaatcggt tgcaaagaca cgatgaatat
     3661 ctttaatttc tcttacaggt tcttctagat aaaggatatt tttaagacga ttggcaaatt
     3721 tcttgacttc catcatttgg atagcatttt taaaatcaat gcgaccagaa cgagatgacc
     3781 caaccaaaat caagcccttt tctaaggcat cgcgagtatt gagattgact ttatattcgc
     3841 taactcccat catgagaatc gttccctgag gacgaatgta gcgaatcaag tcattaatag
     3901 ctggtccagt accatcacca ccacaacatt caaaagcatg gtcaaaggcc aactcttcag
     3961 gaatattatc cgtaatatag cattctttgg caaatgagaa gagttccaac ttttcccaat
     4021 gacgaccaat aaccacaatc tctgcttctg gcaaagtata gttgataata ttggcaacca
     4081 caaaagctaa acttccatct ccaataacgg cgatccgctc ccgcttgcta tgagcaagag
     4141 tcaatagacg attcatagcg tgcatgccca cactgacaaa ctctgtaatg gctgcaaccg
     4201 tatcttcaat agcatcataa gccaccacac gatctttagg gagagaaaca aactctctca
     4261 taaagccatc aaatccacta gacaagaaat gggtccctgt catgtagttt tcatagaatt
     4321 cttcatcact ctgcatagga gactgattgg gaatcatgac aactttttga ccaacctcgt
     4381 aggttccggt cgggtcagaa atgacggttc cacatgactc gtgaatcatt gccattggaa
     4441 gctttttatt caaaatcttg ggatcacgtt ttccctgata gtaacgctga tccgcatgac
     4501 agacagccat gtagttggga cggataagga tatgattctc ttggtcaata gcctcttcct
     4561 gatatttgac attgataaac ttaggcttag ttagttgata aatttgatta atcattttac
     4621 tagtctttct caatcatact ttttgcaatc ttcaaatctg ttacggttgt aatcttcaga
     4681 tttgagtatt cacctttggc caaggccaca tcttttcctt tgatcacaaa gattttacat
     4741 gcatctgtca agatttcctt ctcttcatca gaaagagatc cataaaggtc catgaagtcc
     4801 ttgcaacgga atgtttgagg tgtttgtcct tgataaaggt gagcacgatt tggaatatct
     4861 gtaataaatt gaccattggt actttcaacg atagtatcaa ccgcttctac cactgtgtcc
     4921 actgcgtcat gattttgggc aagttggata ctgtcctgaa tcatgcgaag tgtaataaat
     4981 ggacgaacag aatcgtgggt aacaacgata tcctctggag taagcggacg ataagcatca
     5041 atggcttcaa tgattttctt aatacttgta ttgcggtcag caccaccctt tgtaatgatg
     5101 atacgttcct tataaagagg aagatattta tctacaagat cttctgcatg agaaacccag
     5161 tctccatgaa caccaactac aattttttca atacttggct ccaagacaaa tttttcaatt
     5221 gtatgaatca aaataggtcg atcacctagc tctaaaaatt gttttggcaa gttactgatc
     5281 cccatgcgtg tgccagttcc accggcaaga attcctgcat aaatcatcta ttttctcctt
     5341 tgtcttacta aaaaaactta ttctctctat tataccacaa tctagtccta tcataaaaga
     5401 aatactggtc ctccccttct agaataggag aattatgcag ttctattatt caaagaaact
     5461 agtccatttc tagtaatgac taggaattct gtagcaatta ctaataaatg gctgcgaaat
     5521 tttagagtcc ttcagaataa tatactttcc ttaaagaaaa cttaaaactt aagatttgaa
     5581 acttcaaagt gattacttga cttactgccc ttcatttctt attagctaac tttatgatat
     5641 aatgaaaaac gaggtaaatt gaatgaaaag tataaaatta aatgctctat cttacatggg
     5701 aattcgtgtc ttgaatatta tttttcccat cctaactgga acctatgtcg cgcgtgtctt
     5761 ggaccgaact gactatggtt acttcaactc agtcgacact attttgtcat ttttcttgcc
     5821 ctttgcaact tatggtgtct ataactacgg tttaagggct atcagtaatg tcaaggataa
     5881 caaaaaagat cttaacagaa ccttttctag tcttttttat ttgtgcatcg cttgtacgat
     5941 tttgaccact gctgtctata tcctagccta tcctctcttc tttactgata atccaatcgt
     6001 caaaaaggtc taccttgtta tggggattca actcattgcc cagatttttt caatcgaatg
     6061 ggtcaatgaa gctctggaaa attacagttt tctcttttac aaaactgcct tcatccgtat
     6121 cctgatgctg gtctctattt tcttatttgt taaaaatgaa cacgatattg ttgtctatac
     6181 acttgtgatg agtttatcga cgctgattaa ctacctgatt agttattttt ggattaaaag
     6241 agacatcaaa cttgttaaga ttcacctaag tgattttaaa ccactctttc tccctctgac
     6301 agccatgtta gtctttgcca atgccaatat gctcttcact tttttagatc gcctcttcct
     6361 cgttaaaaca gggattgatg tcaacgttag ttactatacc atagctcagc gaattgtgac
     6421 cgttatagct ggggttgtaa caggtgcaat tggagtgagt gtgcctcgtc tcagttacta
     6481 tctggggaaa ggagacaaag aagcctatgt ttctctggtt aatagaggta gtcgaatctt
     6541 taacttcttt atcattccac tgagttttgg actcatggtt ttaggaccaa atgccatcct
     6601 actttacggt agtgaaaaat atatcggagg cgacatcttg acctctctct tcgcttttcg
     6661 tacgattatc ctggccttag ataccattct tggttcccaa attctcttta ccaatggcta
     6721 tgaaaaacgt atcacagtct atacagtctt tgctgggcta ctcaatttgg gcttgaatag
     6781 tctcctcttt ttcaaccata tcgtggctcc cgaatactac ttactgacaa ctatgttatc
     6841 agaggcttct ctacttgttt tctatatcat tttcatccat agaaaacaac tcatccactt
     6901 gggacatatc tttagctata ctgttcgata ctctctcttt tcactttcct ttgtagcaat
     6961 ttatttcctg attaatttcg tgtatcctgt ggatatggtc attaatttgc catttttgat
     7021 taatactggt ttgattgtct tgctatcagc tatctcttat attagtctac ttgtcttcac
     7081 aaaagatagc attttctatg aatttttaaa ccatgtccta gccttaaaaa ataaatttaa
     7141 aaaatcttag gagtttaaaa tgaaacaact aaccgttgaa gatgccaaac aaattgaatt
     7201 agaaattttg aattatattg atactctctg taaaaagcac aatatcaact atattattaa
     7261 ctacggtact ctgattgggg cggttcgaca tgagggcttt atcccttggg acgacgatat
     7321 tgatttatcc atgcctagag aagactacca acgatttatt aacatttttc aaaaggaaaa
     7381 aagcaagtat aagctcctat ccttagaaac tgataagaac tactttaaca actttatcaa
     7441 gataaccgac agtacgacta aaattattga tactcgaaat acaaaaacct atgagtctgg
     7501 tatctttatc gatattttcc ctatggatcg ctttgatgat cctaaggtca ttgatacttg
     7561 ttataaacta gaaagcttca aactgctgtc tttcagtaaa cataaaaata ttgtctataa
     7621 ggatagcctt ttaaaagatt ggatacgaac agccttttgg ttgctccttc gactggtttc
     7681 tcctcgttat tttgcaaata aaatcgagaa agaaattcaa aaatatagtc gtgaaaatgg
     7741 gcagtatatg gcttttattc cttcaaaatt taaggaaaag gaagtcttcc caagtgctac
     7801 ctttgataaa acaatcgatt taccctttga gaatttaagc cttcctgcac ctgaaaaatt
     7861 tgatactatt ttgacacaat tttatggaga ttatatgacc ctaccaccag aagaaaaacg
     7921 cttctacagt catgaatttc acgcttataa attggaggat taggatgcaa tatttagaaa
     7981 aaaaagaaat taaagaaatt caactagccc tgctggacta tattgatgag acttgtaaga
     8041 aacatgatat tccttatttt ctcagttatg gaaccatgct tggagccatc cgccacaaag
     8101 gtatgattcc ttgggatgat gatattgata tttcccttta tcgtgaggat tatgagcgtt
     8161 tactgaagat tattgaagaa gaaaatcacc ctcgctacaa ggttctttcc tacgatacat
     8221 cttcttggta cttccataat ttcgcatcga ttttggatac ttctactgtt atagaagacc
     8281 atgttaagta caagcgtcat gataccagcc ttttcatcga tgtcttccca attgatcaat
     8341 ttacagactt gagcattgtc gacaagagct ataagtatgt ggcccttcgt caactagctt
     8401 acatcaaaaa atcacgagca gttcacggtg atagcaaact aaaagatttt cttagattat
     8461 gtagctggta cgctctccga tttgtcaatc ctcgctactt ttacaagaaa attgatcaac
     8521 tagtcaaaaa tgctgtaacc aacattcctc aatatgaagg aggagttggg atcggtaagg
     8581 aagggatgaa agaaatcttc ccagttgata cctttaaaga actgatttta actgagtttg
     8641 agggccgtat gttgcctgtt cccaaaaaat atgaccaatt tttaacccag atgtatggcg
     8701 attatatgac accaccatca aaagaaatgc aagagtggta tagtcatagc attaaagctt
     8761 atcgcaaaaa ctgattgagg gggattatac aaactactaa gatagaggtt attcaaaaac
     8821 ataattttag tagaaaatga aatacatatt cccacaataa aacgcatcat atcaaggttt
     8881 ttgaaaaacc ttgatatgat gcgttttata attttaaaga cttttttcta tagtagattg
     8941 aaataagatg cgaacaaatc aattagaaaa ttcaaattaa tttctagaaa tattttagta
     9001 ttcctgtgta ctgttctaaa ttcagtctgc tatatcttat ttttctattt aaatcgcttc
     9061 tgtaacaaag ctacgacttt caagtacctt aagcatggca ttagctgtat ctagcgctgt
     9121 gaagaggggc accccgtgtt caatggctga acgacgaatt tgctcaccat cttcgtcagc
     9181 agttcgtttt gttcctactg tgttaatgat agcttgaatt cttcctttgc gtacaaaact
     9241 tgggatatcc ttatcgtcat caccaatctt accaacaggt tgggcttgca agccatggct
     9301 agcaaagaag gctgctgtcc cttctgtcgc aaggattcca taaccaatgt tttggaaacg
     9361 acgagccaag ttcaaggctt cttctttggc atcatcagcg atggtaaaga cgacattacc
     9421 aaaagttggc aagtgtagat aagaagcttc aaaggcttta tagagagctt tttccaaagt
     9481 agcatcagaa cccataactt cacctgttga cttcatttca ggaccgagca agctgtctac
     9541 cttagctagt ttggtaaagg agaagacagg tgccttgata tgaacacggg tgctttcagg
     9601 gtaaagtcca ttttggtagc caagttctga taaactttga ccaagaatga gtttggtcgc
     9661 tacttgagcc ataggaatat tggttacctt agataggaat ggaacagtac ggctggcacg
     9721 tggattgacc tcaataacgt agactttttc atccttgata acaaactgga tgttcatcat
     9781 tccaaggcag tgaagaccga ttgctaagcg tttggtgtag tctgcgatgg tctcctgaac
     9841 cttttgcgac aaggtttgtg gtgggtaaac agccattgag tcacctgagt ggacaccagc
     9901 acgttcgata tgctccatga taccaggaat gagtacattt ttaccatctg aaatggcatc
     9961 aacttcgcac tcttgcccaa cgatataaga gtcgacaaga actgggtggt ctggactagc
    10021 cttaacagca gttcgcatgt aagaacgaag gtcttcttcg ttttcaacga tttccatggc
    10081 acgtccacca agtacataag atgggcggac aagaactggg aagccaatct tgcgagctgc
    10141 aagagctgct tcttcttcat tggtagctgt ttgtcctggt ggctgtggaa tatccaattc
    10201 tttgagagct tgctcgaaga ggtcacggtc ttcggcacga tctaggtcag caacctgtgt
    10261 accaaggatg gtcacacctg cttttgccaa tggctccgca aggttgatgg ctgtttgacc
    10321 accgaactga acgataactc cctttggttg ttccaagtca atgacgttca taacatcttc
    10381 gaatgtcaat ggctcaaagt aaagcttatc tgatacagag aagtctgttg aaacggtctc
    10441 tgggtttgag ttcatgatga tagcttcata accagctgcc tggatagcct taacagagtg
    10501 aacggttgcg tagtcaaact caaccccttg accaatacgg attggacctg aacctaggac
    10561 aagtacagat tctttatcag atctgataga ttcattttcc caaccatagg ttgaatagaa
    10621 atatggcgtt tcggagtcga actctgccgc acaagtgtct accatcttat aaactggaac
    10681 aatcttgttt tccaagcgaa gttggcgaac ttgatcatca gtcgttcccc agagttcagc
    10741 aatcttacgg tctgaaaaac cattaagttt ggctgttttc aaaacttcta gatcttgtgg
    10801 atgagcaccc aattcttgct caatttcaaa gatatgcaag agtttatcaa gatagaagat
    10861 atcaattttt gtaagctctg caatttcttc aggtgtgtag ccacgacgaa ttgcttctga
    10921 tacgtagaag agacggtcat cttgggcttt gacaaccttt tcaatcaagg catcatcaga
    10981 aactgctgca agttcaggta tttcattgtg gtgcacccca atttcaaggg agcggcaggc
    11041 cttgagaaga gattcctcga tgttacgacc gattgccatg acttctccag tcgccttcat
    11101 ttgtgtaccg agacggcgtt cacccttttc aaacttgtca aatgggaaac gtggaatctt
    11161 agcaactacg tagtcaaggg ctggttcaaa catggcatag gttgaacctg taactgggtt
    11221 tataacctca tccaaggtca aacctactgc aatcttggca gccaacttag caatcggata
    11281 tcctgtcgct ttagaagcaa gggctgacga acgtgataca cgagggttta cttcgataac
    11341 ataatacttg aagctgttag gatcaagagc tagctgaaca ttacatccac cttcaatctt
    11401 gagggcacga ataatgctca agctcgcatc acgaagcatt tggttttcat agtctgacat
    11461 ggtttgcgca ggggcaaata caatggaatc ccccgtgtga atcccaactg ggtcaaagtt
    11521 ttccatgtta caaacaacca aggcattgtc agctgagtca cgcatcactt cgtattcaat
    11581 ttccttgaaa ccggcaatcg aacgctcaat caaacattgg gtaacaggtg acaatttcaa
    11641 accattttca gtgatttcac gcaattcttt ctcgttggca cacataccac caccagtacc
    11701 accgagggta aaggctggac gaacgatgac tgggtagcca attgtcgctg caaaggcaac
    11761 tgcttcttct actgtgttaa caatttcaga ttctggaatg ggttgttcaa gctcttccat
    11821 caattgttta aagaggtcac ggtcctccgc ttggtcaatg gcagataatt tggtacccag
    11881 aagttcaacg ccaagctcat ctaagatacc atttttagac aattccatgg ccatgttgag
    11941 acctgtctga ccaccgagtg ttggtagcaa ggcatctgga cgttccttac gaagaatacg
    12001 tgtcacaaac tcaagtgtaa tcggttcaat gtaaaccttg tcagcaattt ccttgtccgt
    12061 catgatggtt gcaggatttg agttaaccaa aacaacctca taaccttcct ctttcaacga
    12121 caagcaagcc tgagtcccag cgtagtcaaa ctcagcagcc tgaccaataa taatcggacc
    12181 agaaccaatc accataattt tttgaatatc agtacgttta ggcatagttt atgatacaaa
    12241 gcccgtaaag aacacagtga aaataggaaa ctcggcgcag acgccttcag cgtcaagacg
    12301 atgtttatct ttttcacaca gttcttaggg cgtgttcact tccgcgaaca atgtatcttc
    12361 tcctttctat tcgtcgcctc tcagggcgac attaaataag atacaaagga cgactagaaa
    12421 gcgattgaat tttaggaaat caaggaagga ttgacaatcc aagttggttt ctctaaattc
    12481 tgagctttcc gtccatgttc agttacataa attctccgac gagcttttac tcattcttag
    12541 tttgattgtt taaaaacttc catcatctcg ataaactcgt caaataggta gctagcgtcg
    12601 tgtggcccag gagctgcatc tgggtggtat tgaacagaga aagcaggttg gtatctgtgg
    12661 cgcacacctt ccactgactt gtcattgatt tcttcgtggg taataatcaa gtgctctggc
    12721 aaatcctcgc ggctgactgc ataaccatgg ttctggctgg tgaagtctac tcgtcctgtt
    12781 gcgatttcac gtaccgcatg gttgaatcca cggtggccaa acttcatctt ataggtctta
    12841 gccccgtttg ccattgcaaa gagttggtgt cccatacaaa taccaaagat tggaattttt
    12901 ccttgtacac cgcgaatcat gtcgagtgct tgtggaacgt cttctgggtt acctggacca
    12961 tttgacaaca taactccatc aggattgaga tggagaattt cttcagccgt tgtcgaataa
    13021 ggaacaactg tcacgttaca gttgcgttta gaaagttcac gtaggattga gtgcttgaga
    13081 ccaaagtcca ctagcaccac gctcaaacca actcctggag ctggataaga cgttttagta
    13141 gaaacctgtt tgatattgtc tgtcggtaaa actgttgctt ggagctggtc cgtcacatgg
    13201 tccatactgt ccccaacatg ggtcaaggtt gcacgcatag taccatgctt acggataatc
    13261 ttggtaagag cacgcgtatc aattcctgaa atccctggaa ttttcttggc tttcaaaaat
    13321 tcatccaagg tcatttggtt gcgccagttg ctagctctac gcgcttcttc aaaaacaacg
    13381 actcccttac aagttggaat aatggattca taatcatcac gattaatacc ataatttcct
    13441 accaaaggat aagtaaaggt caagatttgt ccattataag actggtctgt aatggattct
    13501 tggtagccgg tcatccctgt attaaagacg atttcgcctg ttacatcaat atctgctccg
    13561 aaggccttgc cttcaaaaac tgtgccatct tctaatacta gaattctttt tgtcatattt
    13621 tcacctctcg tggacgctca ctggcgtctt ttaacgtctt gtgttttagt tggcgtttct
    13681 actcgctagt acggattcta agattgccat tcgaacaaag acaccattgg tcatttgttg
    13741 gacaatccgt gattttggtg cttcaaccaa gtggtctgct atttctacat cacgattgat
    13801 tggagctggg tgcatgagga ttgctgtttc tttcaaacga tcgtaacgtt cttgagtcaa
    13861 gccatgttgg gcatgatagt cttcttttga aaatacagct ccactatcat ggcgttcgtg
    13921 ttgcacacgg agaaacatca tgacatcaac ctgatcaatg atttcatcaa tggttacaaa
    13981 ctttccatag tctgcaaact cttgacttct ccattcctca ggtccagcga aaaagagttc
    14041 agctcccaag cgtttcaaaa tctgcatatt ggatttggca acgcgtgagt ggtccaagtc
    14101 acctgcaata gcaactttaa gaccctcaaa gtggccaaat tcctcataaa tggtcatcaa
    14161 atcaagcaag ctctggctag ggtgttggcc cgaaccatct ccaccattga tgatggaagt
    14221 cgtaatcgtt ggactagcaa tcaattctct atagtagtcg acctctggat ggcgaatcac
    14281 acagacatcc actcctaaag cagacagagt caaaatggtg tcataaagtg tctcaccctt
    14341 attaaccgaa ctagtcttca catcaaagtc aagtcgttcc aatccaagtt taatctctgc
    14401 gacttcaaag gacttatgtg tccgtgtaga atcctcaaag aagagattgg aaacaatcgg
    14461 atggtcttca tagggaagct gggctccatt tttaaactca attcctcgct tgatcaattt
    14521 cattacttga tcgacagtga ggtcttccat ggacaccaca tggttcaatg cttgttgatt
    14581 ttctgacatg gctactcctt taactttcta agcttcttca gtaatcagaa ctctgtcttg
    14641 gtcatcaagt tctgtcatct ctacgatgat ttcttcagaa cgactggttg ggatattttt
    14701 tccaacgtaa tctggacgga ttggcaattc tctatgtcca cgatcgacta gaactgctaa
    14761 actcacacgc gcaggacgac catgaccgac aatattatca atagcagcac ggatggtacg
    14821 acctgtatag agcacatcat ccaccaagat aacttcgcgg tctgtcacat cgacagaaac
    14881 caaagaagta tcttctccac ttttaacatc atcacggaaa ggtttagtat ccaattccac
    14941 aacaggaact gaaagatttt ctaactgctt caaacgttct tggattcggt gggcaataaa
    15001 gacaccacga gttttaatac cagccaagac gatcttattc aaatctttgt tgcgttcgat
    15061 aatctcataa gtaatacgcg taatcgctcg tttgacggtc aattcgtcta caacttcttt
    15121 tgttttcatg acaaacctcc aaaaagaaaa gtctccttaa acaaggagac ttgaaattta
    15181 tagccaagcg agccctactg caaacagtat agacttcacc cttctacttt atcgcgctcc
    15241 ttgcctgcct cacgggacag gtttaaagga atatttagtt atcatttact atagcacaaa
    15301 gcatgcttaa aatcaagcaa aaagtttcaa tgtagcatct tacaaattgc taaaatcata
    15361 taattgtggg tactggtcac actctggatt ttttggatgg caaatggctc ttccaaaata
    15421 aatcatggcc tgatgggcag ctaaccactg ctcaggcggc aagatatcca tgacccgctt
    15481 ttccacctca agtggcgtcg ctgatttttt gacaatatcg tggtgtttgc aaatacgctc
    15541 cacatgagta tccactgcaa aggctggaat cccaaatcct acactcatga caacattggc
    15601 tgtcttgcga ccaacacctg ccaaactctc caattcttca cgtgtctgag ggacttgacc
    15661 atcaaaatcg tctagtaact gttgggcaca ttttttaagg aatttagctt tattccgata
    15721 caatcccaag cgagaaatat gtgaagcaat ctcactctct gtcgctacag acatagcttg
    15781 gggtgttgga aaggcaacaa agagacctgg tgtggcctta tttaccgctg catctgtcgt
    15841 ctgggctgat aacatgaccg caaccaggag ttcaaaatga ttggtaaaat caagactagg
    15901 cttggcatct gggaagaggg caatgatttc ttctagcacc tttcgtgctc gtttttttga
    15961 caagaccatt attcatctcc gtcaaatagt ccttgtaagc cagcaaaagg actgttttct
    16021 tctttcttta ctgcttgttg agcttggtat tcttcctctg tcatgatttg ccagtcattt
    16081 cctgagataa atccttgacc agcttcttct tcagccgtca agaccttgat aggaatgttt
    16141 agcaggatat tgtctgatac actctcagc
//
LOCUS       DAVBCG010000036        16069 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBCG010000036 DAVBCG010000000
VERSION     DAVBCG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.036"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(365..1138)
                     /locus_tag="VQ495_000959"
     CDS             complement(365..1138)
                     /locus_tag="VQ495_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2530933.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            1314..2717
                     /locus_tag="VQ495_000960"
     CDS             1314..2717
                     /locus_tag="VQ495_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2530934.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            2832..3470
                     /locus_tag="VQ495_000961"
     CDS             2832..3470
                     /locus_tag="VQ495_000961"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2530935.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            3482..3922
                     /locus_tag="VQ495_000962"
     CDS             3482..3922
                     /locus_tag="VQ495_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2530936.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            3915..4346
                     /locus_tag="VQ495_000963"
     CDS             3915..4346
                     /locus_tag="VQ495_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2530937.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            4337..4807
                     /locus_tag="VQ495_000964"
     CDS             4337..4807
                     /locus_tag="VQ495_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2530938.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            4832..5605
                     /locus_tag="VQ495_000965"
     CDS             4832..5605
                     /locus_tag="VQ495_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2530939.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            5602..6402
                     /locus_tag="VQ495_000966"
     CDS             5602..6402
                     /locus_tag="VQ495_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2530940.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            6461..8755
                     /locus_tag="VQ495_000967"
     CDS             6461..8755
                     /locus_tag="VQ495_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2530941.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            8765..11881
                     /locus_tag="VQ495_000968"
     CDS             8765..11881
                     /locus_tag="VQ495_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2530942.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDSNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            11938..13704
                     /locus_tag="VQ495_000969"
     CDS             11938..13704
                     /locus_tag="VQ495_000969"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2530943.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            13850..15001
                     /locus_tag="VQ495_000970"
     CDS             13850..15001
                     /locus_tag="VQ495_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2530944.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            15146..16030
                     /locus_tag="VQ495_000971"
     CDS             15146..16030
                     /locus_tag="VQ495_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2530945.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         5288 a         2368 c         3433 g         4980 t
ORIGIN      
        1 tatatttaga tcatcaaggt cacttaattc ttgaaatata gggtctaggc gacaaagtct
       61 ttaaaatcag aaaaacgcat aatatcaggt gtgcaaaaac ttgatactat gcgttttatt
      121 gtgggaaggt ttactccatt ttctcctgaa attgagtttt tgtccagcct ctgtttttag
      181 aattgctaag aaaataatgt catgtggtga atatttgtaa atcagtcagc agacagaacg
      241 aatactcttc gaaaatctct tcacatcatg tcagcttcgt cttgccgtgt ccatggggga
      301 cttgacttga tcagttttca aatctgtact ttgagcaagc tgagactagc ttcctatttg
      361 attttcattg aatatcagaa acccattctc catcaaataa ttcgactgcg tctaataatt
      421 tttggtctgg caaggtgtca gaaataaagg ttgtgtattt ggagagggga ttaattttaa
      481 aaaatccagt cttgtaaaat ttagaactat caatcagtaa gatggtttca tgggctttgt
      541 caataatatt cttttttgaa atagcttggc tgagagaagc ttcataaaca tattggtcat
      601 caatacctct tgctgaacaa aatgctaaat cgatattaaa atgatctaat aaagaatttt
      661 ccttatcata gttgaccacg gaacaggatt gatgtttgac ctcgccagat gtgataaaga
      721 ttttggagct atctttaaca gtttcagata gggtttgtgc agtatgtaaa ccatttgtaa
      781 aaataatcaa attatcaagt tcagaaagat agggacaaag ttcgtagaca gtagtactag
      841 aatctagata gatacacata ccagaccgaa taaagtcttt agcgagacta gcgattagtc
      901 ttttttgcct agtactttct ccttcccgta tttgatgaga aagttcaatt gtgttcatag
      961 aggacagggt cacgtatccg tgctttcttt tgataagacc ttgattttct aagaaaatta
     1021 aatcacgacg taaggtactt gtgctggaga aagtgatttc tgccagctct tttacggcaa
     1081 ttcttttttt ctttttaata atttcaatca attcaagtac acgttcatct tttatcataa
     1141 gctcctccta atttatcatt tcaactatat tatagcacaa attggaggaa tttgaattat
     1201 ttttatgaat attgggttaa catttgaaca ttattcaagt aagcgttcac atattgaaaa
     1261 aataaaacgt ggggattata ataaagttaa tcaaggacga agagagaaga aaaatggaag
     1321 cggttttagc aatagattta ggtgcgactt ctggaagagc aatcgttggt tacctttctg
     1381 aaaataaact agtaatggaa gaaataaatc gcttttctaa tctacctatt agagtaaaag
     1441 ggcatttatc ttgggatatt gactttctac tagctaaaat tcttgaaagt atccgcttgg
     1501 ctaatactag ttacaagatt ttatctatcg gtattgacac atggggagtt gattttggac
     1561 tgattgataa tgaaggtaag ctgttatcac aacctgttca ttatcgtgat gaaagaacaa
     1621 agggagtgtt aaaggaaata tctgaaatga ctgaattaga aaaactgtat tcagagacag
     1681 gaaatcagat tatggagata aataccttgt ttcaactctt taaggcacgt caagaatctc
     1741 ctgactcttt ctataagacc aataagattc ttttaatgcc agatttgttt aattatctct
     1801 tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac aactcaatta tttgatccta
     1861 ggagtcaaaa ttggaatcag aatatcttaa aactatttga attggattca tctttacttc
     1921 ctgaaattgt ttcagaggga aatgttcttg gaaggataaa aaaggagtat ggtttaggcg
     1981 atattcctgt tgtgaatgtt tgtagtcatg atacagcaag tgcgattgtc tcagtaccta
     2041 agacagaagg tagtttattt atttcatcag gtacttggtc tttggttgga gtggaactta
     2101 cttcaccgat tcttactacc gaatccttca gttatggatt tacaaatgaa gtcggtaaag
     2161 atggagtgat tacatttctg aagaattgta cagggttgtg gatcatagag gaactaagac
     2221 gttcatttga acgaagaggg aaagcctatt cttttgatga tattaggaca atggtggaga
     2281 aagaaaaaga aaatcttcct ctgattgata ctgaatcaac tgaatttgca acagaatctg
     2341 atatgcacaa gactttgaca gaatatctag cttatcatca tgaaactaga gagtggacag
     2401 atggacaact atttaagatt gtttatgaaa gcctagctga aacgtatagg aaagcgatag
     2461 agttattaga agaactaact cataaggttt ataagaggat atatgtgatt ggaggaggtg
     2521 ctagagccag ttattttaac caaatgattg ctgatagaac tggtaaagag gttcttacag
     2581 gtttgactga ggctacagct gtggggaata ttgttgtgca gctcatagct atgggacaat
     2641 taaaagggat ggaagaggct caccatgtta ttgaggagtt tctacaatta gagagttatt
     2701 actcccaaaa gaattaaaaa gattgagagt ttgtaaattt gcctccctcc cccttcttag
     2761 cttttgtgca ggaagggggg ataattggtg aattgaaaaa tatttagtgt tttgatatga
     2821 ggaggacaag gatgtcagat gtaaaacaag aattaattaa atatggtaag aagctagtag
     2881 aaacagattt gacgaaagga acaggtggga atctcagcgt tttcgatcgt gaaaaacaat
     2941 tgatggcaat taccccgtcg ggtattgatt tctttgaaat caaagaatcc gatattgtag
     3001 tgatggatat taatggaaat gttgtagagg gagaacgctt gccatctagc gaatggtata
     3061 tgcatttgat tcaatatcaa actcgtgatg atatcgatgc aattatccat gctcatacaa
     3121 cttatgcaac agtattagct tgtctcagag aaccacttcc agcgagtcat tatatgattg
     3181 cagtggcagg gaaagatgtt cgggtagctg agtatgcaac atatggcacg aaagaattgg
     3241 ctgtgaatgc agctaaagca atggaaggtc gtagagcagt tttactagcg aatcatggaa
     3301 ttttagcagg tgcacaaaat ttattgaatg catttaatat tgttgaagaa gttgaatatt
     3361 gtgcaaaaat ttattgttta gctaagaatt ttggagagcc agtagttctt cctgatgagg
     3421 agatggaatt gatggcagaa aaatttaaaa catacggtca gagaaaatag ggaggatatt
     3481 aatgttaaaa catataccga aaaatatttc tccagattta ttgaagactt taatggaaat
     3541 gggacatgga gatgaaatag tattagctga cgcgaattat ccttctgcct catgtgcaaa
     3601 taagctaatt cgttgtgatg gcgtaaatat tccagaatta ttagattcca ttctgtattt
     3661 aatgccatta gatagttacg tcgatagttc aattcagttt atgaacgttg tttcgggtga
     3721 tgatattcct aagatatggg gtacctatag acagatgatt gaaggtcatg gtacagatct
     3781 taaaacgatt acttatctta gaagagaaga cttttatgaa cgtagtaaga aagcttatgc
     3841 tattgttgct acaggagaaa cttcacttta tgctaatatt atccttaaga aaggagtagt
     3901 tgttgaaaga gaaaatgttt aatagaggaa ttttagttgc cagtcatggt gattttgcta
     3961 gcggagctct catgaccgca gaaatgtttg ttggtgagac aacaaatgat agagttagga
     4021 cattaggttt gatgcctgga gagaatattg tagagtttga gcattatttt aaaaatcaag
     4081 tggatgaact gttagactca aatcaagagg ttatcgtttt gactgacttg attggaggaa
     4141 gtcctaataa tgtggctttg tcacggtttt taaatttgga ttcagttgat attgtaacag
     4201 ggtttaatat tcctctccta gtggaattaa tatcaaatta tgattcaaaa atcaatttag
     4261 aagaaattgt tcacaatgct caaaatagtt tgtttaatgt taaacaacaa cttaacgtag
     4321 aggaggaaga agatttatgt ctatagagtt tgttcgtatt gatgaccgtc tggtacatgg
     4381 tcaagttgtc actacgtggc taaaaaagta tgatattgag caagttatca ttgttaatga
     4441 tcgcatctca gaagataaaa cacgacaatc tattttaaag atttctgcac cagtaggttt
     4501 aaaaattgtt ttctttagtg taaaacggtt tgtggaagtt ttaaactctg tgccaataaa
     4561 aaagagaaca atgctgatat atacaaatcc aaaagatgtg tatgattcta ttgaaggaaa
     4621 tttaaaattg gagtacctca atgtaggaca gatgagtaaa acggaggaaa atgaaaaggt
     4681 aacgggaggt gtagctctag gtgaagaaga caaatattat tttaagaaaa tagttgataa
     4741 gggaacgaga gttgaaattc aaatggttcc taatgataaa gttacaatgt tggaaaaatt
     4801 tttataaaaa taatttaagg aggtacagta tatgctattc acacaagcat tactggtgac
     4861 attagttggg attattgcca ctattgacta taatggaccg ttatttatga ttcaccgtcc
     4921 gttagttaca agtgcaatgg ttggcttagt cttaggagat ttcacccaag gtgttcttat
     4981 tggttcagct cttgaattaa cttggctcgg tgtaacaggt attggaggtt acactccacc
     5041 agatactatt tcaggtgcga ttattggtac tgcatttggt attttatctg gtcaaggaga
     5101 aactgctggt atcgctatag cagttccaat tgcagttgct acccaacagt tggatgttct
     5161 tgcaaaaact ttagatgttt attttgtgaa aaaagctgat aatgatgcta aaaacggaga
     5221 ttattcaaag atcggttttt atcattattc aagtttggtt ttaatcacgt tatttaaaat
     5281 tgtaccaatt ttcctagcta ttatgcttgg aggggaatat gtggcagact tgtttgctaa
     5341 ggttccacca atcgttatgc agggacttaa ctctgcaggt gctttactac cttcaattgg
     5401 ttttggtatg cttttaaata tgatgctcaa gaaaaatatg tgggtattct tgttgattgg
     5461 attcatttgt tctgtgtatg gaggaatgtc aaccattggg atctcactag ttggtattgc
     5521 ggtagcatac ttctacgata tgattggaag taaaccacaa gaaacaactt caagtagtga
     5581 tgttgaggag gatcttgatc tatgatgaat aataaagtaa ctaaagttga acttaaaaaa
     5641 gttttcaaac gaagttttat gtatggttct tcatggaact atgagagaat gcagaaccta
     5701 ggttttctat atacaattct tccagtattg aaaaaactat acccagacaa agattcagct
     5761 tctcctgcaa tgaaacgtca ccttgagttt ttcaatactc atcaaacagc ggcaccattt
     5821 attcttggag ttacttccgc tatggaagaa caagaaggaa atgaaggtgc agcttcaatt
     5881 actggtatta aagttggctt gatggggcca ctggctggtc taggagatag tttgttctgg
     5941 ctgacactag ttcctatctg ttttagtatt ggtgcgtctt attctaaaga cggcggtgct
     6001 ttaggtatct ttatcgcctt aatattgttt aatattatta atattcctgt taaatatttc
     6061 ggtttgaaat atgggtatac taagggttct agtcttatcc aagaaaataa tacaaaagga
     6121 acattgaatc gcgttacgag tatggcgaca gcattagggc tagtactagt gggtggtttg
     6181 attccatcaa tggttggtat taattttgga ttagaattta agcaggggga acttgttatt
     6241 tctgttcaag aaatgattac aaaattaatt ccaggattta tccctatggc tttgacttta
     6301 ttaatgtgta aattaattag aaaaggaaag aatccggttg tactaatctt tagtgttatg
     6361 gctattggag ttatcctagt tgttttagga attttgaagt agtagaaagt gtggaggtgg
     6421 tatttgggat atcacctcca ttttggaaga gaggtaaaga gtgaaattat ggtataagaa
     6481 agctgcctca aattggaatg aagccttgcc gattgggaac ggtcatttag gtggtatgat
     6541 ttatggttca gctacaaaag aatgtattca actaaacgat gagactattt ggtatagagg
     6601 aaagtcagat agaaataatc cagactcact attgcatctt aaaaaaattc gggaatatct
     6661 tttagatgga gaaattcaga aagccgaaga attgataaag ttaacaatgt ttgctacccc
     6721 aagagatcaa agccactatg aattacttgg ggaactttac attgagcata tagatattca
     6781 gtcttgtgct ctttcattgt atgaaagaga gctagattta gatacagcta tttctaatgt
     6841 tgtgtttgag cctaatagtt gtaatttaca aataaaaaga gaatatttta cgagttttaa
     6901 taagaatatt ttatgttgcc gtatagtgtc atcagttcaa aacacattaa atttaaacat
     6961 taatttgggt agaaataaac ggtttaatga cgaagtatct aaactggatt caagtacaat
     7021 tttaatgtcg gcctctgctg gaggtagaaa aggtgttcag tttaaagtag tatgtcattc
     7081 taaggttacg gatggtgaag taagtgtatt gggagagaca atagttattc ggaatgctac
     7141 agaggtattt ctttatctca aatcaatgac ggattattgg ggaaatatag atatttcttc
     7201 tcttcaggga gaatttagta gtattgatta ctttacagaa aaagatgaac atgtaaaaaa
     7261 atatcaggag caatttaata gagttgattt taaactagac tatagtaaag attgtcttag
     7321 cattccaacg aatctacttc ttgaaaacac taaaaagtat agtaactact tgactaactt
     7381 gttatttcat tatggaagat atctgttaat atcgtctagt caaccgaatg gtttacctgc
     7441 caatcttcaa ggaatatggt gtgatgaatt aaatccaatt tggggttcta aatatacgat
     7501 taatattaat actcaaatga attattggat ggtaggtcca tgtgatttac cagaagtaga
     7561 atatccatta tttgatatgc tcgaaagaat gagagaaccg ggaagactaa ccgctaagaa
     7621 aatgtatgga gctagaggtt ttacagcaca tcataatacg gatggttttg gcgatacggc
     7681 tccccaatct catgccatgg gggctgcaat ttgggtatta actattccat ggttatgtac
     7741 tcatatttgg gaacactatt tatatttcca agatgagcgt attcttacgg aacattttga
     7801 aatgataaaa gaagcatttc ttttctttga agattattta tttgaggtgg atggctactt
     7861 gatgacaggt ccaagtgtct caccggaaaa taaatatcgc ttaaaaaatg gtattgaagg
     7921 aaatgcttgt ctatcatcta caattgataa tcaaattcta agatattttt gtgattcatg
     7981 cattggcatt gcaaaacaat taggagacaa ttcggatttt attagtcgtg tgaaggagtt
     8041 aaaaaagaaa ctacctaaaa caaaaatagg tagtaatggg caaatccaag aatggttaga
     8101 agattatgaa gaagtagagc ctgggcatag acacatttca cctctatttg ggctttatcc
     8161 ttataatgag attgatattc ataaaactcc ggaattagca gaagcagcta aaatcactat
     8221 caataggaga ttatcaaacg ctaacttttt atcttcacag gagagggagc aagcgattaa
     8281 taattggtta gtaagtggtt tgcatgctag tacacaaaca ggttggagtg ctgcatggct
     8341 gattcatttt tttgcgagac tatatcaagg tgaacctgct tataaccaga ttaatggttt
     8401 gttaaataat gcgactcttg gcaatttatt tcttgaccat ccaccatttc aaattgatgg
     8461 taatttaggt ttggtgagtg gaatttgtga attattagta cagagccatc ataattggtt
     8521 atcactaatt ccagctttac cttctgcttg gtcagaagga gaagtgaaag gtttcagagt
     8581 aagaggagga tataaggtat cgtttgcttg gaaaaatggg gatataacat tcctaaaatt
     8641 ggaaggagga aacaaagatc aaaaagtaag agtaagaata tatggcaaaa atactgatgt
     8701 acaaaatatt gaattggtat ttaattcaga aaaaattatt gagttaaatt tttaggtata
     8761 agtcatgaat aaagaaaaaa taaaaagaaa attaatcaca atactgtttg tatgtattgg
     8821 gatgttatgt tttggattgt tagcaggagt taaggctgat aatcgtgttc aaatgagaac
     8881 gacgattaat aatgaatcgc cattgttgct ttctccgttg tatggcaatg ataatggtaa
     8941 cggattatgg tgggggaaca cattgaaggg agcatgggaa gctattcctg aagatgtaaa
     9001 accatatgca gcgattgaac ttcatcctgc aaaagtttgt aaaccaacaa gttgtattcc
     9061 acgagatacg aaagaattga gagaatggta tgtcaagatg ttggaggaag ctcaaagtct
     9121 aaacattcca gttttcttgg ttattatgtc ggctggggag cgtaatacag ttcctccaga
     9181 gtggttagat gaacaattcc aaaagtatag tgtgttaaaa ggtgttttaa atattgagaa
     9241 ttattggatt tacaataacc agttagctcc gcatagtgct aaatatttgg aagtttgtgc
     9301 caaatatgga gcgcatttta tctggcatga tcatgaaaaa tggttctggg aaactattat
     9361 gaatgatccg acattctttg aagcgagtca aaaatatcat aaaaatttgg tgttggcaac
     9421 taaaaatacg ccaataagag atgatgcggg tacagattct atcgttagtg gattttggtt
     9481 gagtggctta tgtgataact ggggctcatc aacagataca tggaaatggt gggaaaaaca
     9541 ttatacaaac acatttgaaa ctggaagagc tagggatatg agatcctatg catcggaacc
     9601 agaatcaatg attgctatgg aaatgatgaa tgtatatact gggggaggca cagtttataa
     9661 tttcgaatgt gccgcgtata catttatgac aaatgatgta ccaactccag catttactaa
     9721 aggtattatt cctttcttta gacatgctat acaaaatcca gctccaagta aggaagaagt
     9781 tgtaaataga acaaaagctg tattttggaa tggagaaggt aggattagtt cattaaacgg
     9841 attttatcaa ggactttatt cgaatgatga aacaatgcct ttatataata atgggagata
     9901 tcatattctt cctgtaatac atgagaaaat tgataaggaa aagatttcat ctatattccc
     9961 taatgcaaaa attttgacta aaaatagtga ggaattgtct agtaaagtca actatttaaa
    10021 ctcgctttat ccaaaacttt atgaaggaga tgggtatgct cagcgtgtag gtaattcctg
    10081 gtatatttat aatagtaatg ctaatatcaa taaaaatcag caagtaatgt tgcctatgta
    10141 tactaataat acaaagtcgt tatcgttaga tttgacgcca catacttacg ctgttgttaa
    10201 agaaaatcca aataatttac atattttatt gaataattac aggacagata agacagctat
    10261 gtgggcatta tcaggaaatt ttgatgcatc aaaaagttgg aagaaagaag aattagagtt
    10321 agcgaactgg ataagcaaaa attattccat caatcctgta gataatgact ttaggacaac
    10381 aacacttaca ttaaaagggc atactggtca taaacctcag ataaatataa gtggcgataa
    10441 aaatcattat acttatacag aaaattggga tgagaatacc catgtttata ccattacggt
    10501 taatcataat ggaatggtag agatgtctat aaatactgaa gggacaggcc cagtcccttt
    10561 cccaacacca gataaattta atgatggtaa tttgaatata gcatatgcaa aaccaacaac
    10621 acaaagttct gtagattaca atggagactc taatagagct gtggatggta acagaaatgg
    10681 taattttaac tctggttcgg taacacacac tagggcagat aatccctctt ggtgggaagt
    10741 cgatttgaaa aaaatggata aagttgggct tgttaaaatt tataatcgca cagatgctga
    10801 gactcaacgt ctatctaatt ttgatgtgat tctatatgac aataatagaa acgaagttgc
    10861 taagaaacat gttaataatt tgtcgggtga atctgttagt ctagatttca aagaaaaagg
    10921 agcaaggtat attaaagtta aattactaac gagtggagtg cctttgagtt tagcagaagt
    10981 agaggttttt agagaatcag atggtaagca atctgaagag gatatagata aaataacaga
    11041 agataaagta gtctctacaa ataaggtagc tactcaaagt tcaaccaatt atgagggtgt
    11101 agctgcttta gcagttgatg gtaataaaga tggagattac ggacatcatt cggtgactca
    11161 tactaaggca gattctaacg cttggtggca ggtcgatctg ggagaagagt ttacggtttc
    11221 taaagttgat atttataata gaacagatgc cgaacctcag cgtttatcta attttgatgt
    11281 tatttttcta tcttcatcag gagaagaagt ttttagaaga cattttgata aagtagttga
    11341 tggtttgtta tctttaaaag taccttctgt aggggctaag ctagtcaaaa tagaattaaa
    11401 atcagcagct attccgttaa gtttagcgga agttgaagtc tatggttcaa agagaactcc
    11461 gaagaaactt tctaatattg cattaaccaa agaaactcga cagagttcaa cggattacaa
    11521 tggtttttct cgtctagcag ttgatggaaa taaaaacgga gattatggtc atcattcagt
    11581 gactcatacc aaaggagatt ctccttcatg gtgggagata gatttagcac aaaccgaaga
    11641 attagaaaag ttaattattt ataatagaac agatgctgaa attcagagat tatcaaattt
    11701 tgatattatt atatatgatt caaataatca tgaagttttt aaacaacata ttgacagttt
    11761 agaaagcaat aatctatcca tagacttaaa agggctgaag ggaaaaaagg ttagaatttc
    11821 tttgagaaac gcaggaattc ctttaagttt ggcagaggta gaggtttata cttataagta
    11881 attttaaaaa ttatcatcgg tagtatgaaa gaaacagaaa aataagagga aaatagtatg
    11941 attcaacatc cacgtattgg tattcgtccg actattgatg gtcgtcgtca aggtgtacgc
    12001 gaatcacttg aagtacaaac aatgaacatg gctaaaagtg tggcagattt gatttcaagc
    12061 acattgaaat atccagatgg ggaacctgtg gaatgtgtga tttctccatc taccattggt
    12121 cgtgttccag aggctgcagc ttcccatgag ttgtttaaaa aatcaaatgt ttgcgcaaca
    12181 attacagtta caccatgctg gtgttatggt agtgaaacta tggatatgtc tccagatatt
    12241 cctcatgcta tttggggatt taatgggaca gaacgcccag gagctgtcta tcttgcagct
    12301 gtactagctt cacatgctca aaaagggatt ccagcctttg ggatttatgg tagagatgtt
    12361 caggaagcta atgatacaga tattccagaa gatgtcaaag aaaaactttt acgttatgcg
    12421 cgtgcagctc ttgcaactgg cttgatgaga gacactgctt acctatcaat gggtagtgtt
    12481 tcgatgggga ttggtggttc tattgtaaat ccggatttct tccaagaata cttaggaatg
    12541 cgaaatgaat cggtagatat gacggagttc acacgccgta tggaccgtgg tatttacgac
    12601 cctgaagagt ttgaacgtgc tatggtttgg gtaaaagaac atatcaaaga aggagttgac
    12661 cgaaatcgtg aggatttgat tctttctaaa gaagagaaag aaaaacaatg ggaatttgtt
    12721 attaagatgt tcatgattgg acgtgactta atggttggta acccaagact tgctgaactt
    12781 ggttttgagg aagaagcagt tggtcaccat gctttagtag ctggtttcca aggtcaacgt
    12841 cagtggacag accattttcc aaatggagat tttatggaaa ctttcctcaa tactcagttt
    12901 gactggaatg gtattcgaaa accatttgta tttgcgacag agaatgattc actaaatggt
    12961 gtgtctatgc tctttaatta tctattaaca aatactccac aaatctttgc tgatgtgcgt
    13021 acttattgga gtccagaggc tgttgaacgt gtaacaggac atactttaga gggtcgtgct
    13081 gcagctggct tcttacatct aatcaactct ggatcttgta cattggatgg tacaggtcaa
    13141 gctactcgag atggcaaacc tgttatgaaa ccattctggg agttggaaga aagtgaagtg
    13201 caggctatgc ttgaaaatac agacttccca ccagcaaacc gcgaatactt ccgtggagga
    13261 ggattctcaa ctcgtttctt gacgaagggg gatatgccag taacaatggt acgtctcaat
    13321 cttctaaaag gggttggtcc agtgctacaa attgcagaag gttacacact tgaacttcct
    13381 gaagatgttc accatacttt agataatcgt acagatccag gatggccaac tacttggttt
    13441 gctccacgtt tgacaggaaa aggtgctttc aagtctgtct atgacgtcat gaataattgg
    13501 ggagctaatc acggagccat aacatatggc cacattggag cagacttgat taccttggct
    13561 tctatgttga gaattcctgt caatatgcat aatgtacctg aggaagatat ctttagacct
    13621 aaaaattggt ccttatttgg aacagaagat ctagaatcag cagactatcg tgcatgtcag
    13681 ttgttgggac cactacataa ataaaacttg tttatatagg aggtgaactt acgtccctcc
    13741 tatcctttta aaaagatttg ttaaacaatt cacaaataat tgaaaacgaa tacaaaaagt
    13801 aatataatga tgttaaatag atagcgcaaa ggcgcaggag gaaaattata tggctacatt
    13861 ttatgttccg gcagtcaacc ttattggtaa aggtgttgta aatgaagtgg gtccttatat
    13921 caaggaactt ggctataaaa aggcactttt ggttacagat aagtacatcg aaggcagtga
    13981 cattttacct aaggttttaa aaccactgga tacagaagga atcgaatatg tcatctttag
    14041 cgatgtagag ccaaacccta cttgtaagaa tgtcacagat ggggtagctg ctttgcaaga
    14101 acatggatgt gattttatca tcagtcttgg tgggggttct ccacaggatg cagctagttg
    14161 tatttctatc atggctacaa atggtggaag accacaggat tatgaagggc ttcataagtc
    14221 tgctaaaaaa ggcttgccag ttgtggctat caatacaacg gcaggtacat cagcagaaat
    14281 taccattaac tatgtgatta ctgatgaaga acgcaaggtt aagatggtaa tggttgacaa
    14341 gaatagcctt gccttgattt ctgtcaatga ccctgaactc atgctttcca aacctaaagg
    14401 cctgactgct gctactggta tggatgctct gactcatgct gttgaagctt tggtaacacc
    14461 tggtgcttat gatgtaacca agaaactgtc tattggtgct attgagctta tcaaggaata
    14521 tcttcctcgt gctgtagaaa atggacatga tattgaagcg cgtgaaggta tggtcaatgc
    14581 catcttcctt ggtggtatga gctttaataa tgctggtctt ggctatgttc actcaatggc
    14641 tcaccaactc ggtgcagtat ataatttgcc acatggcgtg tgctgtgcca tgttgctacc
    14701 agttatagaa cgtgaaaatg ctaaacgtgt accagaagct ttccgcaatg ttgccaaagc
    14761 cttgggactt catgtagaag gtaaatcaga tcaagaatgt gccgattatg cgattgctga
    14821 gattgagaag ctttctgaga cagtaggtat tcctaagaaa ctgactgaac ttggtattga
    14881 agaaaaagat ttcgactttg aatacctttc taagaatgcc ttgattgatg cctgcgcacc
    14941 aggaaatcca tttatgccaa ccttagaaga aacgattgcc ttttataaag agttatttta
    15001 gcaagagatt ggacgacaat aggacaattc acctctaatt attgacattt caggaaaaat
    15061 cgcagtaaaa acggcacagc cttctttaaa tgtgctataa tactagaaaa atacttgtgg
    15121 aggttccatt atggcaatat ttttcatgat ttttctgatt gtttgtgtgc tcctattggt
    15181 gatagtcaca ctgagtacag tttatgtggt tcgtcagcag tcggtggcga ttattgaacg
    15241 ctttgggaaa taccaaaagg ttgccaatag cggtattcat attcgcttgc cttttgggat
    15301 tgactcgatt gcagcacgga ttcagttgcg cttgttgcaa agtgatattg tggttgagac
    15361 taagaccaag gacaatgtgt tcgttatgat gaatgtagcg actcagtacc gtgtcaacga
    15421 gcagagcgtg acagatgctt actataaact catacgtcca gaatctcaga ttaaatctta
    15481 tatcgaagat gctcttcgtt cttctgttcc aaaattaacc ttggatgaat tgtttgagaa
    15541 aaaagatgag attgcccttg aagttcaaca ccaagtagca gaagaaatga ccacttacgg
    15601 ctacattatc gtgaaaacct tgattaccaa ggtcgaacca gatgcagaag ttaagcaatc
    15661 tatgaatgaa atcaatgcgg cgcaacgtaa gcgggtcgca gcacaagaat tggcggaagc
    15721 tgacaagatt aaaattgtca ctgcagctga agccgaagca gaaaaagacc gccttcatgg
    15781 tgtggggatt gcccaacaac gtaaggcgat tgtggatgga ttggcagagt ctatcaccga
    15841 actcaaggaa gccaatgttg gaatgacaga agaacaaatc atgtctatcc tcttgaccaa
    15901 ccagtatttg gataccttga atacctttgc ctctaaagga aatcaaacca tctttttacc
    15961 aaatactcca aatggtgtgg atgatatccg aacacaaatc ttgtcagccc ttcgcgctga
    16021 gaagaaataa tagactaata ctcttcgaaa atctcttcaa accacgtca
//
LOCUS       DAVBCG010000037        15430 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBCG010000037 DAVBCG010000000
VERSION     DAVBCG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.037"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            221..559
                     /locus_tag="VQ495_000972"
     CDS             221..559
                     /locus_tag="VQ495_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2530946.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQETLDKHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            749..2185
                     /locus_tag="VQ495_000973"
     CDS             749..2185
                     /locus_tag="VQ495_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2530947.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVI
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGENREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            2237..2476
                     /locus_tag="VQ495_000974"
     CDS             2237..2476
                     /locus_tag="VQ495_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2530948.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2606..2920
                     /locus_tag="VQ495_000975"
     CDS             2606..2920
                     /locus_tag="VQ495_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2530949.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            3038..5017
                     /locus_tag="VQ495_000976"
     CDS             3038..5017
                     /locus_tag="VQ495_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2530950.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            5027..5341
                     /locus_tag="VQ495_000977"
     CDS             5027..5341
                     /locus_tag="VQ495_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2530951.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5372..5878
                     /locus_tag="VQ495_000978"
     CDS             5372..5878
                     /locus_tag="VQ495_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530952.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFENLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5958..7304
                     /gene="celB"
                     /locus_tag="VQ495_000979"
     CDS             5958..7304
                     /gene="celB"
                     /locus_tag="VQ495_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2530953.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNSLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7684..7860
                     /locus_tag="VQ495_000980"
     CDS             7684..7860
                     /locus_tag="VQ495_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530954.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            8262..10475
                     /locus_tag="VQ495_000981"
     CDS             8262..10475
                     /locus_tag="VQ495_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET2530955.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEDEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10697..11173)
                     /locus_tag="VQ495_000982"
     CDS             complement(10697..11173)
                     /locus_tag="VQ495_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2530956.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTNPKQIEEAIQTLL"
     gene            11396..14599
                     /locus_tag="VQ495_000983"
     CDS             11396..14599
                     /locus_tag="VQ495_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2530957.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSTWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDIASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKSGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14979..15364
                     /gene="tnpA"
                     /locus_tag="VQ495_000984"
                     /pseudo
     CDS             14979..15364
                     /gene="tnpA"
                     /locus_tag="VQ495_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         5140 a         2250 c         3170 g         4870 t
ORIGIN      
        1 actcacctga gtacaatcct attgagaaaa catgggctct tttgtcctgc tcttgtttca
       61 attgactata aatcgctgta agaaaaaatc attttattgg tataaagaag tgattgaaag
      121 taatggcgaa acgttagata attagttcat aaaaaacaag tttagctcct catctaaact
      181 tgttttttta aaattgaagg atgattttgg aggatataag atgacattga ctattcatgt
      241 atattataaa ggtgatggag atagtgcaat aaagtttgca agagaaatgc ttgatagtgg
      301 ccttgttgaa gaaattagaa atcaaaaagg taacttgaaa tacgaatatt ttttaccaat
      361 tgaaaaagag ggaactattc tccttattga tcagtggata aatcaagaaa ctttagataa
      421 gcatcatcaa tcaaagacaa tgcagaaaat cttagattta agaaaaaaat atcacttgca
      481 gatgcaagtt gaacgttata tagaagatga ttctgggatg ccagaaagcg ataagtcgtt
      541 tatagacaca gggaattaga attattgtga gagtttttgt agtttgaaag tattgaaaat
      601 cgtaaagata tcattacgag aatacaagaa gatattgttc tatttccatt aaaaattact
      661 tctttcctta acaataagga aaaagaatgc gttttcatct tttggtttga ggtttataat
      721 aagagtatca aagtcaggag gaaaaaagat gactactttt aaagatggat ttttatgggg
      781 tggtgctgtt gctgctcatc aacttgaagg tggatggcaa gaaggtggca agggaattag
      841 tgttgctgat gttatgactg ctggtcgtca tggagtagct cgtgaaatta ctttgggagt
      901 tttagagggt aaatattatc caaatcatga ggcgatagat ttttatcatc gttataaaga
      961 agacatagca ctttttgctg aaatgggatt caagtgcttc cgtacctcta ttgcatggac
     1021 acgtatcttt ccaaaaggtg atgagttaga gccgaatgaa gaaggattac agttttatga
     1081 taatcttttt gatgaatgct taaagaatgg tattgaacct gttatcactc tatctcattt
     1141 tgaaatgcct tatcacttag tgaccgaata tggtggttgg aaaaatagga aattgattga
     1201 tttctttgct cgttttgcag aagtcatatt taagcgttac aaagataagg ttaaatattg
     1261 gatgactttc aatgaaatca ataatcaagc gaattatcag gaagattttg caccatttac
     1321 taactcaggt attgtatatg aggaaggtga gaatagagag gcaattatgt atcaagcagc
     1381 acattacgaa ttagttgctt ctgcacgagc tgtaaaaatt ggtcatgaga ttaatccaga
     1441 ttttcaaata ggttgtatga ttgcgatgtg tccaatttat ccagctactt gcaatcctaa
     1501 ggatatctta atggcaatga aagctatgca aaagcgttat tattttgctg atgtgcatgt
     1561 tttaggtaaa tatcctgagc atattttcaa gtattgggaa cgaaaaggta tttcagttga
     1621 ttttactgcc caggataaag aagatttact tggtgggact gtagattaca ttggtttcag
     1681 ttactatatg tcctttgcta tcgactctca tcgtgaaaat aatccttatt ttgattatct
     1741 tgaaacagaa gatttagtga aaaataatta tgttaaggct tctgaatggg agtggcaaat
     1801 tgatccagaa ggtttgcgtt atgcgttaaa ttggtttaca gaccactatc acttaccact
     1861 ctttattgtt gaaaatggtt ttggagctat agatcaagtt gcagcagatg gtatggtaca
     1921 tgatgattat agaattgaat atctaggtgc ccatattcgt gaaatgaaaa aggctgtagt
     1981 tgaagatggt gttgatttaa tgggttatac tccatgggga tgtattgatt tggtttcagc
     2041 tggtaccggt gaaatgcgga aacgttatgg ctttatttat gtagataaag atgataatgg
     2101 gaagggaagt tataatcgtt ccccgaaaaa atcttttggc tggtataagg aagttatttc
     2161 atctaacggt gaatcagtag aatagtataa acaaactgag attattctca gtttgtttag
     2221 catattgagg tgacatatga ttggaagtat tgcagcaatt ttaacaacat ttgcattttt
     2281 accacaagtt tttcgtgtag ttaagacaaa ggacacagga tctattgctc taggtatgta
     2341 tgttatgcaa gttattggta ttgcattgtg gcttgctcat ggtattcgta taggagactt
     2401 gcctttgatt ttagcaaata gtgtttcatt tctactctca ggaataattt tattttataa
     2461 attgaagtat aaataatttt tttccatatc atttaggaaa atgagggaat tgtcattgga
     2521 aacgctttat tttataataa aaatagataa caaataagcc aacttagcac agctttgatt
     2581 ggatttaaga aaaggagaaa atattatggc aaaagcttta attatttgtg cagcaggaat
     2641 gtcttcatca cttatggcac agaaaacaac agagtttcta aaaggaaaag ggaaagatat
     2701 tgtagttgat gctattagtg ctactgaagg agggaaagct atcgcagcag cagcttttga
     2761 tttgtatctt gtcagccctc aaacaaaaat gtactttaag caatttgaag aggctggttc
     2821 taaagcagga aaaccagttg tacaaatacc tcctcaagcc tatattccta ttccaatggg
     2881 aattgaaaaa atggctaatt tgattcttga gaatatttga tgaacacatg aaatgtcaat
     2941 aaatatttaa gttaagataa attgttttga gtttgatata aattgattta agtgtataat
     3001 atgcttcaat atatgaaaac atacgtttta ggagggaatg aatgtgttaa acattaaaga
     3061 gaaagatatc cttcaatttt taataaaaaa taaggaacgt tttgttacaa gcaaagaact
     3121 ggcagaatat ttatcctgtt cagatagaac tgtccgtaat gtactgaaat taattgagaa
     3181 gacaatgatt attcaaggag tccggttgat ttcaaaacaa ggtcaagggt atcaaatatt
     3241 ttttgagaat caaggcgctt accaggagtt tagacaaact tatgaactgg aagaagacta
     3301 tacgaaaact gcggtttcaa aaggtgatga tcgtttagta tttatcttaa ataaattatt
     3361 atttgagcaa gttccagttc tttttgatga tttagctgat gaactttacg ttagtcgttc
     3421 aacattatct catgatttca gaaagatacg agtgatgtta tcagagtata atttgtccat
     3481 tgagagtagg gcgaataaag gagtttatgt ctcaggcgag gaacgtgata aacgacgttt
     3541 tatcataaat tatttcctag agaatcaatt ttttaaaaca atacattgct atgtaaaatt
     3601 taactttttt gctcaaactg ttccattaga agaatttgct agaattgttt tggatgaatg
     3661 ccaagaggcc aacctaaaac tatctgattt tgtactacag aatttagtag ttcacatagc
     3721 attgtctgtg attcgtttaa aatccggatt tgaaataaaa aatatagact gtcagatgac
     3781 tgatgatgca atagagagaa aggttgctca aagaattctt tctaaagtaa gagaagtaac
     3841 aaatcaggaa tttccagttc aggaaataga ctacattact ttgcatttgt tagctaagag
     3901 tcaacaatgt caaaaaaatc agaagaatat ttctgaagaa gttttaaaaa aatctttatt
     3961 taaaacattt cagaatttag gattagatga tatgtataac ttttcttcag atttccaact
     4021 aattgaagga ttgataactc atctaatgac actacaagta cgtttagaaa gccggattac
     4081 cttaaataac cctttggtag atgaaattaa acagaattat agtgatatat tctttatgac
     4141 tagagaaatt cttgcaaata tggacaagtt cttggagtgg acaatatcag atgatgaagt
     4201 agcatatgta agtcttcact ttttagctgc tatggaacgt agcaaagaga gtactaagtt
     4261 taatattctt gctatttgtg caacaggatt tggtgcagcc caaatgttaa gaaatcgttt
     4321 ggaaacggag tttgggaagc gagtagaagt tgttgatgta ataggctatt atgaattgaa
     4381 tcaagaaaaa cttaaaggaa tagattttat agtgtcagct gttgatttat caaatctata
     4441 ttttcaaatt ccagttttta aagttagtgt gtttcttaag agtgatgaga tggaaatgat
     4501 tagaaaagct atggatcaga tgcaagtttc tagttatgta caatcatcaa agattaataa
     4561 atttgaaaac aacagcttta gacagtattt tagtaaagaa aattttttaa tatgtactga
     4621 atcagacaag gtaaatttac ttgaaaagat ggttgagagt ttatctgttg gtgaatcaaa
     4681 tgagtttgaa caatctttac tttatggaat taaacagcgc gaagaattaa gttcagttgt
     4741 tttttctgaa aaaattgcag ttccgcatcc tattcagccc tttggtacag agggaaaggt
     4801 ttcggttgca atttgtaaag attcgttact ctgggataat caaagttcat atgtccagct
     4861 agtatttctt ctatctccat caatatatgg gaacgaaggt ttggcaactg tgactaaaaa
     4921 gatagtctct ctgacagaga atgatgaatt gcaaaatcag ctaatatctt gcaataactt
     4981 tgaagatttt attaacatat ttgagaagat aaaataggag gttcatatga atcaagagga
     5041 actgcaggtt gcagcatttg aaattattct gcattcggga aatgcaaggt cagaaataca
     5101 tgaagcattt gcaaaaatga gagaaggtag ttttgatgat gcagaaagta aattaaatca
     5161 atcaaatgag ataattcttg aagcgcatca tgcacaaacc aaactcttgc aagaatacgc
     5221 aagtggagtg gaaattaaaa ttgaaattat catggtacat gctcaagacc atttaatgac
     5281 cactatgaca ttattagaag tagcaaagga aatgttagct ctttataaga aagttaacta
     5341 aatatgtgtt gttatataag gaggaaatga tatggataaa aaagaacgac aaaaaattga
     5401 acaacaacgt cgagagatgg ctctaactaa tacttttttt aatcgatatc ttcttctacg
     5461 ttattcaata gcactcttct tttttgggaa tatttattgg cttttgaatc agtttattag
     5521 tccctcgcct attattattt tccccattat gctcatagta ttttctattt tagcaactat
     5581 tgaacaattt aaactttatg gaaataggaa agaaaagttg ggaataacac taatgtttgt
     5641 tagaatacaa atgcttattt caataggatt acttgtttta acttggacaa gttggtttga
     5701 gaatcttttt ccgatttttg aaaataacca agtagcacgt ttatttgttt ttgtagttct
     5761 tctattaggt ttagttctga gtttgttaga cattagaaga attaaaaaaa tttataaacg
     5821 aacagataag gcttatcaac aatttgtcca attggaaaag aactcactta gtttataatc
     5881 ctgaactagt ataatttgta tttgaaacta gtctttaaaa agtaaaaatt attatgtttt
     5941 attaaggagg tctatttatg gatcaacaaa acgggttgtt tggttttctt gaaaaccatg
     6001 ttatgggacc aatgggcaaa cttgctcagt ttaaagtagt gcgtgctatc acggctgcag
     6061 gtatggctgc tgtaccattt actattgtag gatcaatgtt tttggtattc agtattttgc
     6121 cacaagcttt ctcattttgg ccaattgtgg cagatatttt ctctgcttca tttgataaat
     6181 tcacatcact ttacatggtt gcaaactatg cgactatggg ttctctatct ctttatttcg
     6241 ttctatcact tgcatatgaa ttgacaaaaa tttatgcaga ggaggaagaa ctcaatatga
     6301 attctcttaa tggtgccttg ctcgccttga tggcttttgt catgacagta ccgcaaatca
     6361 tttttgatgg tggaatgatg aagactgtga caagtctaaa agaaggtgca gtaattgcag
     6421 atggatgggc aatgggaaat ggagtcgcac gttttggtac aacaggaatt tttaccgcaa
     6481 tcattatggc aattgtgact gttcttattt atcgtatgtg tgttaaacat aattgggtta
     6541 ttaaaatgcc tgaagctgtt ccagaaggag tttctcgtgg atttaccgct ttggttccgg
     6601 gatttgttgt tgcatttgtt gttatcttta tcaacggtct tcttgtagca atgggaacag
     6661 atatttttaa agtcattgca attccatttg gttttgtatc caatctgact aattcgtgga
     6721 ttggtttaat gattatttat ctattgactc aactactttg gattgtaggt atccacggtg
     6781 cgaacattgt ttttgcattt gttagtccaa ttgctcttgc taacatggct gaaaatgctg
     6841 ctggcgggca cttcgctgtt gcaggtgaat tttctaatat gtttgtaatt gcaggtggtt
     6901 ctggtgcaac tttaggacta tgtttatata ttgcttttgc ctctaaatct gaacagctta
     6961 aagcaatagg acgagcatct gtagttccag ccttatttaa tattaatgaa ccattaattt
     7021 ttggattacc tattatctat aatccagcct tggctatacc atttatttta gcaccaatgg
     7081 ttactgctac tatttattac gtagcgaatt ctctaaactt tattaagcca attatcgcac
     7141 aggttccatg gccaactcca gtagggattg gagctttctt agggacagca gatcttcgag
     7201 ctgtattagt tgctctagta tgtgcatttg cagcattcct agtctatctt ccattcatcc
     7261 gtgtatatga tcaaaaattg gtgaaagaag agcaaggtat ctaataagta aaaaagtata
     7321 ctcgatgata gaatccatat agtttaaata atgagcgtta acattagcag tgaagaggtc
     7381 tttttaagat attcttctct gctttttcat attttttcgc tatgtagagt atgaagcaaa
     7441 atagaaagta gttcgaatag ttgataaaca gggaattgct gttatttaaa aaaaacaata
     7501 aagtaaagta tgttatcgta gaatacgata caatagtaaa aaagcaaaaa gaatgcgttt
     7561 tatatttggg tttgaggctt ataaaatagg ataagcgtac tgaaaactta gatatgacaa
     7621 acaaaataaa atttttaaaa gctacagaga aacatggtat aatgtatcta aaggaggtag
     7681 catatgtcct tagatataga taaagaaaag atgacaatca tgggaatagc ttttgaaaac
     7741 cgatccgttt ttaagagtgt ttggtatgct ttaagtacaa atatgattga aggatggcga
     7801 cctacggtta gtgatgttga aaaattacga gatgaagctc tttctctggg gatgacttaa
     7861 tgaagcgcaa ctgttatgag tcttatgact atgttgatcc ggataattta tataccgatc
     7921 cgggttcttc agttctaaga aataagcagg aaggaaaaaa gcacgcgagc ttgaatatcg
     7981 tatgattgct agtaagagtt tgaaattatt tatcaatcct atcttagtta atagtttaaa
     8041 agtagtttgt ttcacaactg cataagctta gaaaagttga aaagtgataa ctctttaata
     8101 aactcttggt ttgttaaaag atattttgtc cactacattt ttttcaattg actattattt
     8161 gtcatagata taacagatag aacattgcat tcagaataat gaaaaacgca tcaaaaatgg
     8221 tgcgttctgt ttttatgcta ccataaaagg aggaaaacaa attgaaaatt tttaagggag
     8281 agttttatcg aatctctgta ttaacagaca agctagtaag gttagaatac tctcgaactg
     8341 gaaattttga ggatagaacg acacaactta tctataatag agattttggc caagtttcgt
     8401 tagattatat cgagacatca aatgtactag atattatgac ggactatttt catctgcact
     8461 ttaataaagg agaatttaac gccgaaaatt tatttataga attaaaagga aattttgccg
     8521 tatatggtag tcgctggtat tttggtgaat ctattgaaac gttaaaagga acagctcgga
     8581 ctctggataa ggcagatgga gcaatctcgt tagaagatgg aattattagc cgaaatggta
     8641 tagccttatt ggatgattct caaggattta tttgggatga acaatctggt tatattgaga
     8701 gagaaaatca aattgacctg tatttctttg cctatgggca tgattataga ggagcaatca
     8761 gagactttta ccatttgact ggttcaacac ccttgttgcc aagatatgct ttaggcaatt
     8821 ggtggagtag atattggcct tatacgtcgg atgaatactt ggatttaata gacagatttg
     8881 aaacagagaa aattccatta tctatcggtg tgttagatat ggattggcat ataactgaca
     8941 ttccagctcg ttttggaagt ggctggacag gatatagttg gaatagaaac ttaataccaa
     9001 atccagaaca gttattgcaa caacttcatg atagaaagtt aaaactctcc ttaaatgtcc
     9061 atcctgctga tgggatacgg gcttatgaag aagcttatcc tcaaatcgca aaacggttgg
     9121 ggttaaatgt agaactagaa gaacctgcta tttttgattt ttttaatccc tcttttaggg
     9181 aagcctactt taaagatgtt cattatgagc tagaaaagca gggagtagat ttttggtgga
     9241 ttgactggca acaagggaca caaggtatgc tagatccact ttggctttta aaccattatc
     9301 actatcagga tagttgtaaa aatgcagaag gtggtttgat tttatcaaga tatgcaggtc
     9361 ctggtagtca ccgctaccct gttggttttt caggggacac tattattagt tggaattcct
     9421 taagatttca accctatttt acagcgacag catctaatat cggttatagt tggtggagtc
     9481 atgatatcgg tggacatatg ttgggggatt atgacgaaga gctacaaact agatggctac
     9541 agtttggtgt ttttagtccg attactcgat tacatagttc tagaagtcct tttaatagta
     9601 aagaaccttg gttcttttca gaaacaacat ctaagattat gaagaaatac cttcgtttga
     9661 gacatcagat gattccctat ctatatacca tgaatgtaaa gacacatgag gaaggtgccc
     9721 cattaatcag tccaatctat tatttctatc cagagaatga tgagagctat aatgttccaa
     9781 accaatactt ttttggaaca gaactgatgg tggctcccat tgtagaaaag atggatttga
     9841 cattccaatc tgcaaaagta gatgtatggt tccctgaaga tgaatggtat gacttctttt
     9901 cagagaaaaa atacacaggt ggtgtgaagt taagtgttta tagggacatc tcgactacgc
     9961 ctgtgtttgc aaaaagtggt gcaatcattc ccttggttgg ttctgagata ggtatgggtg
    10021 ttgatttacc tgaggttgta gattggtatg tattcccagg gaaacaacat tcttttgaaa
    10081 tgcttgaaga tcaaaatggt caaagatata aaacaagatt atcaatcgat tgggaaatgg
    10141 gaatggtaga gttagcatta caaggagatt ctagtatcgt tccaagcaat agaaaacata
    10201 gaattcattt taaaggaacg aatgtgtcta taattgaatt gccaaataag aatgatacag
    10261 ctaaatttga atggaaagat aataaaagga catctttaaa tgacgaagtt tttagactac
    10321 taaagacagc ttctcttcca tatgaattaa aagatagatt gttaaatcaa ttcatcaatg
    10381 cgaaaaactc tcatgactta atgaatatct tgcatcatca ggataaggaa ttgagagggc
    10441 gtttgttgga aatgatattt actagccaaa actaattgaa ataattagaa agtcatttgt
    10501 ttagtgtata tgaagattct gtatggtatt cgttatcact ggtcacattg aaaatgtgaa
    10561 ataagcacct acaacataga ctttacaaca cttcatgttt tatatcatgg ggtgtttttc
    10621 tatttaagtc aaagcccatc aaaaaatctc actattcatc aggttaaagg aaacctaatc
    10681 atagtgagat tgtgaattat agtagagttt gtatcgcctc ttcaatttgt tttgggtttg
    10741 tttttgaaga aaagcgttca aagacttgcc catcacgacc aatgagaaac ttagcgaaat
    10801 tccattcgat tcgttttcct agtgggccag atttatggtc ttttaaccag acatagagag
    10861 ggtctgcttc cttaccgttg accttaatct tggcaaaacg tgggaaggtg gtttgaaaat
    10921 gtaggctaca gaaggcgttg atttcctctg cgctgccggg tgcttgtccc ataaactgat
    10981 tgcaagggaa atccaatatt tcaaagccct gatcttgata gcgttcatag agttcttgaa
    11041 gtccctggta ctggggcgtt aaaccacatc cagtagcagt gttgacaatc aagagaactt
    11101 taccacgata gctatccaag ggagttgctt ggttgttttg gttcaaaacg gaaaaatcat
    11161 aaagtgaagt catgagggcc tcttttcttt ttctacattg taacagtttt tacttttttc
    11221 gtcgagtcgg atggaagata ttgttgaaga ataagccttc aatatgatag aaaccgttta
    11281 caaataactt taaaaatggt atgatttaat taaaccggtt tactttgttg ctataaatct
    11341 ctaatttgag atttagttcc tattgatttt acaatatgtt tattggagtg tatacatgca
    11401 aacaaaaaca aagaagctca ttgtgagttt gtcttcactt gttttatcag gatttttatt
    11461 aaaccattat atgacagttg gagcggaaga aacgactacg aataccattc agcaaagcca
    11521 gaaggaagtt cagtatcagc aaagggatac aaaaaattta gttgaaaatg gtgattttgg
    11581 tcagacggag gacggaagca gtccgtggac aggaagcaaa gctcaggggt ggtcaacttg
    11641 ggtagaccag aagaatagtt ctgcagatgc ctcaactcga gtcattgagg ctaaggatgg
    11701 ggctatcact atctcaagcc atgagaaatt aagggcagcg cttcaccgta tggttcctat
    11761 tgaagctaag aaaaagtata aactgcgttt caagattaaa acagatcata aagtcgggat
    11821 tgccaaagtt cgtatcattg aggaaagtgg taaggacaag cgattgtgga attctgcaac
    11881 gacgtcagga acaaaggact ggcagaccat tgaagcagac tatagcccga ctttagatgt
    11941 tgataaaatc aagctggagt tattctatga aacaggaact gggactgttt cctttaagga
    12001 tattgagctg gtagaggtag cagaccagcc ttctgaggat tctcaaacag ataaacagct
    12061 tgaggaaaag attgatttac caattggaaa aaaacatgtt ttttctcttg cggactatac
    12121 ttataaggta gaaaatcctg acattgcttc agtcaaaaat ggaattttag aacctcttaa
    12181 ggaagggaca accaatgtca ttgtcagtaa agatggcaag gaagtgaaaa agattccttt
    12241 gaagattcta gcctctgtta aggatgcata cacagaccgt ttggatgact ggaatggcat
    12301 catcgctggg aatcaatact atgattctaa aaatgaacag atggccaaat taaaccagga
    12361 attggaagga aaggtagctg atagcctatc tagtatttca agtcaggcgg accgcaccta
    12421 tttgtgggaa aaattttcaa attataaaat gtctgcaaat ctgactgcca cttatcggaa
    12481 attggaggag atggccaagc aagtgaccaa tccttcttct cgttattatc aagatgaaac
    12541 tgtcgttcga acagtcaggg attccatgga atggatgcat aaacatgtct acaatagtga
    12601 aaagagcatt gttgggaact ggtgggatta tgaaatcggt acacctcgtg ccatcaacaa
    12661 taccttgtct ctgatgaaag aatacttctc tgatgaggaa attaaaaaat atacagatgt
    12721 gattgaaaaa tttgtaccag atcccgaaca tttccgaaag acgactgata acccattcaa
    12781 ggctctaggt ggaaacttag ttgatatggg aagggtaaaa gtaatagctg gtttactgcg
    12841 taaggatgat caagaaattt cttctaccat tcgctcgatt gagcaagtgt tcaagttggt
    12901 agaccaaggt gaaggttttt atcaagatgg atcctatatc gaccacacca atgttgccta
    12961 tacgggtgct tatgggaatg ttttgattga tggcctgtct caactgttgc cagtcattca
    13021 aaagaccaag aatccaatcg ataaagataa aatgcaaacc atgtaccact ggattgataa
    13081 atcgtttgct cctttgctgg tgaatggaga gctgatggat atgagtcgtg gacgctcgat
    13141 cagtcgtgca aatagcgagg ggcacgtggc cgcagtagaa gtactaagag ggattcaccg
    13201 aatagcggat atgtctgaag gagaaaccaa acaacgtttg cagagtcttg tgaagaccat
    13261 tgttcaatcg gatagttatt atgatgtctt taagaatttg aagacttata aggatatcag
    13321 tttgatgcaa tccttgttaa gtgatgcagg agtcgcaagt gttccaagaa caagttacct
    13381 atctgccttt aacaagatgg ataaaacagc catgtacaat gcagagaaag ggtttggatt
    13441 tggcttgtca ctcttttcca gccgtacctt gaattacgaa cacatgaaca aggaaaataa
    13501 aagtggttgg tatacgagtg atgggatgtt ctatctttac aatggcgatt tgagtcacta
    13561 tagcgatggc tactggccaa cagttaatcc atataagatg cctggtacaa cagagacgga
    13621 tgctaagaga gcggatagcg atacaggtaa agttttaccg tctgctttcg ttggaacgag
    13681 caaactagat gatgccaatg cgacagcaac catggatttc accaactgga atcaaacatt
    13741 gactgctcat aagagctggt ttatgctaaa ggataagatt gcctttttag gaagcaatat
    13801 ccaaaacact tcaacagata ctgctgcaac tacaattgac cagagaaaac tggaatcaag
    13861 taatccatat aaagtctatg tcaatgataa agaagcctcc cttacagaac aagaaaagga
    13921 ttatcctgaa acccaaagtg tctttttaga atcgtccgat tcgaaaaaga atattggtta
    13981 ctttttcttt aagaagagtt caatcagtat gagtaaggct ttgcaaaagg gagcctggaa
    14041 ggatatcaat gaaggacagt cagacaagga agttgaaaat gaatttctta ctattagtca
    14101 ggctcataag caaaatggag attcttatgg ctatatgctc attcctaacg tggatcgtgc
    14161 caccttcaat caaatgataa aagagttaga aagtagcctc atcgaaaata acgaaaccct
    14221 tcagtctgtt tatgatgcta aacaaggagt ttggggcatt gtgaaatatg atgattctgt
    14281 ctctactatt tccaaccaat tccaagtttt gaaacgtgga gtctatacca ttcgaaaaga
    14341 aggggatgaa tataagattg cctactataa tcctgaaacc caggaatcag ctccagatca
    14401 ggaagtcttt aaaaagctag agcaagcagc tcagccacaa gtacagaatt caaaagaaaa
    14461 ggaaaaatct gaagaagaaa agaaccattc ggatcaaaag aatctccctc agacaggaga
    14521 aggtcagtca atcttggcaa gtctagggtt cttgctactt ggggcgtttt atttattccg
    14581 tagaggaaag aacaactaat ttgttcatca tctagatgaa ttacatgaaa ctgtggaaaa
    14641 tagaaaaaat ccgaagaagg ccaaatatct tcggattttt cttttaaaaa gggataagtt
    14701 gatacttatc cttttttact tcattatgtg actatagcgt tttgaaacta gaataggacg
    14761 tcataactgc taaaagattt ctataaattc atttgatttt cctaatcaat ttgttcgtat
    14821 cctatttcac tccactatat tagtccgtct cgctccgtga ggtgcgagac aaaacaacca
    14881 cccgctatgc gggtgcgcgt cgaaggctat accaaaaaac tccaaacgcg atacaataaa
    14941 ggtgttcaat ccaattgtaa agcgaaagga gaaaaaatat ggcacaaaag actcatagtt
    15001 tatcatacac aaagtggatg tgttctatca tattgtgttc acccctaagt atagacgaaa
    15061 agtcatctat aatcaatata gaagtagttt aggcgaaata tttcatcgct tgtgtggtta
    15121 taaaggtgtt gaaatgatcg agggtcactt aatgccagac catgtacaca tgttagtcag
    15181 tattccacta aggctaagtg tttcgagttt catggggtat ttaaaaggca aaagtgcact
    15241 catgatgttt gacaaacacg ccaatctcaa gtacaagttt gggaatcggc atttctgggc
    15301 ggaaggttat tatgtaagta cagtagggct taataaagcc acacttaaga aatatagtca
    15361 agattaaaga aattatcgag tggatgattt cttcacgagt atgaaaattt gagaacgagt
    15421 aaagtatgat
//
LOCUS       DAVBCG010000038        15156 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBCG010000038 DAVBCG010000000
VERSION     DAVBCG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15156
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.038"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(65..862)
                     /gene="proC"
                     /locus_tag="VQ495_000985"
     CDS             complement(65..862)
                     /gene="proC"
                     /locus_tag="VQ495_000985"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2530958.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(866..2128)
                     /locus_tag="VQ495_000986"
     CDS             complement(866..2128)
                     /locus_tag="VQ495_000986"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2530959.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(2138..3268)
                     /locus_tag="VQ495_000987"
     CDS             complement(2138..3268)
                     /locus_tag="VQ495_000987"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2530960.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(3351..5231)
                     /gene="cbpE"
                     /locus_tag="VQ495_000988"
                     /gene_synonym="pce"
     CDS             complement(3351..5231)
                     /gene="cbpE"
                     /locus_tag="VQ495_000988"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2530961.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SE"
     gene            complement(5234..6121)
                     /locus_tag="VQ495_000989"
     CDS             complement(5234..6121)
                     /locus_tag="VQ495_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2530962.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(6111..6572)
                     /locus_tag="VQ495_000990"
     CDS             complement(6111..6572)
                     /locus_tag="VQ495_000990"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2530963.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMFLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(6569..7477)
                     /locus_tag="VQ495_000991"
     CDS             complement(6569..7477)
                     /locus_tag="VQ495_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2530964.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            7470..7667
                     /locus_tag="VQ495_000992"
     CDS             7470..7667
                     /locus_tag="VQ495_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2530965.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(7719..8390)
                     /locus_tag="VQ495_000993"
     CDS             complement(7719..8390)
                     /locus_tag="VQ495_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2530966.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8444..8665)
                     /locus_tag="VQ495_000994"
     CDS             complement(8444..8665)
                     /locus_tag="VQ495_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2530967.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            8937..9746
                     /locus_tag="VQ495_000995"
     CDS             8937..9746
                     /locus_tag="VQ495_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2530968.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(9927..10802)
                     /gene="aguB"
                     /locus_tag="VQ495_000996"
     CDS             complement(9927..10802)
                     /gene="aguB"
                     /locus_tag="VQ495_000996"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2530969.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKLLAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(10812..11897)
                     /gene="aguA"
                     /locus_tag="VQ495_000997"
     CDS             complement(10812..11897)
                     /gene="aguA"
                     /locus_tag="VQ495_000997"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2530970.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(11894..13021)
                     /gene="nspC"
                     /locus_tag="VQ495_000998"
     CDS             complement(11894..13021)
                     /gene="nspC"
                     /locus_tag="VQ495_000998"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2530971.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(13021..14280)
                     /locus_tag="VQ495_000999"
     CDS             complement(13021..14280)
                     /locus_tag="VQ495_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2530972.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMLD"
     gene            complement(14277..15137)
                     /gene="speE"
                     /locus_tag="VQ495_001000"
     CDS             complement(14277..15137)
                     /gene="speE"
                     /locus_tag="VQ495_001000"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2530973.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         4435 a         3507 c         2771 g         4443 t
ORIGIN      
        1 cgcagtttga atttgatttt cgaagagtat cagccaccat aaaagaggca gataaaacta
       61 cctcttattt acctagttct tgtgttcgtt tataggcttg atgaactgcc tccatgactg
      121 tgcctcgaaa agcatgcgct tctaggcttg ctacgccagc aatagtcgaa ccgcctggac
      181 tacaaacttg atctttcaaa actccaggat gttgctggct ttctaggacc aattgcccag
      241 ctcctaccac agtttgggct gccattttca aggctgtttc tcgtggtaat cccgtctgaa
      301 cacctgcatc tgccaaggcc tcaataaaga gatagacaaa ggctggtcca caacctgcaa
      361 gacctgtcgc tgcattgatt aagctttctc ctaattcaac caagagacca gccttggcta
      421 aaagctgaca aaagatctca ctgtcctcag ccctgcaatt aggagacaag gcataactaa
      481 tcactccttg cccgatagaa gcaggggtat taggcatcat acgaataatt cggtgttggc
      541 ttgggataag acttgctagt ttttctaagg tcaatccagc tgccatcgaa atcaaaagaa
      601 gactttctct tttttcaagg atggtctggt attgagaaag cagttcagaa aactgagcag
      661 gcttaacacc tagaaaaatc acatctgctt cttcgaatat ttcttcattg ttagaagtct
      721 gaccaccgaa gttagcgatg aaagcatcca ccttgacttg actacgattg gcaaggagaa
      781 tctcatctga cggtctagcc tgcaagacag acttggttaa actagcaccc atattcccca
      841 aaccgataaa tccaatcttc atctcttact cccttatctg tccatcacca gtgaccacat
      901 acttgtagct agtcaattcc ttcaagccca taggaccacg cgcgtgcaat ttctgagtag
      961 aaatccccat ttcacaacca agaccaaatt gtcctccatc tgtgaaacga gttgaggcat
     1021 taacatacac cgctgcagag tccacttgat ctgtaaagta tgctgcagct tcagcatttt
     1081 ccgtcacaat ggcatccgaa tgatgggtac tgtgggattc aatatgcgca accgcttctt
     1141 ctaaactgct cacaacctta acagctagaa tatagtctaa aaactcggta tcaaagtctt
     1201 gtgcttgagc agcttgacct gaaacaaact ggcttgcttt gctatctagg cggaattgaa
     1261 ttggttccaa cccagcttct tttcgatctg caaccaacac ttgctccaag cgaggaagga
     1321 agcttactgc tttgttttca tgaaccagca gaacctccat ggcattacaa acagaaggac
     1381 gactggtttt agcattgttg atgatagaca gcgccttgtc ttcgtctgca tccttatcca
     1441 catagacatg gacaatccca gttcctgtct cgataacagg tacaatcgca ttctcaacca
     1501 ctgcattgat caagccagct cctccacgag gaatgagaag gtctagatag cccttggcct
     1561 tcatcatagc ataactactt tcacggctag tatcctccac cagttgaatc acatttggat
     1621 gaatagtagt cgtctccaag cccttcttca aggctgtgac aatggcatgg gttgtttgat
     1681 aggcatcctt accactacga agaacaaccg catttccact cttaagagtc aaagcagccg
     1741 catcagacgt cacatttgga cggctttcat agataatacc gatgacaccc atagctacac
     1801 gtttttttgt gataaccaaa ccattttcaa gctgacttgt ttctaaaact tcaccgattg
     1861 gatctggtaa ggcaaccact tcacgaattc ctcttgccat cgcttctata cgatctgcat
     1921 ccaaataaag acgatccaac atcacatctg agattttccc cttagccgct gccatatcga
     1981 gggcattagc cgctaaaatt tcctcagtag cagccactaa gtgatcagcc atggctagca
     2041 aggcttggtt tttcacttct tcactagctg tgttaatcga ttttttaaca gcctgtacct
     2101 gttcaaattg ttcttgtcta ctcaccatag tttacctcta aaattctgta aaaagtagtt
     2161 ggatttcagg agtaatggaa atccagtcgt cacggtaaat caagacaccc ttggcttttt
     2221 gaaaacgcaa catatcctcc aaagcagatg ctccaaattg cacgcgtcct tttccaagtg
     2281 attttccact ttccttatca aatactgtca cgatatcacc gtaagaaaag actccttctg
     2341 cttcaacgat accagataag agaagactct ttccatgttg agagagagct tccgcagccc
     2401 ctttatcaac ccaaatagaa ccttgactct gagcatagaa ggcaagccat tgtttctggg
     2461 tacgaagccc cttctcttga gcaacaaagt aagaaccatc ctcggtctcc tctgccgcct
     2521 caaccatgga atctgatttc aaggatgagc agatataaac aggaactcct gattccgtcg
     2581 cgatagttgc agccttgatt ttggttaaca tacccccagt tccgtttgac gaaccagctc
     2641 caccagccat atcaataatc tcacgattga tggtctcgat tctctccaag cgtttggctc
     2701 ttggatctga attaggattt ccagtataga gaccgtccac atctgtcaag aaaactaaaa
     2761 ggtctgcttg gaccatcgcc gctacttgag cacttagagt gtcattgtcc ccaaccttga
     2821 gctcatcaat aacgacacta tcattctcat tgatgatagg aattgcccca cggttgagca
     2881 aaaccgacaa agcctgatgg gcatttttat aacgacgctt atccacaaag tcatcttggg
     2941 tcagcaagat ttgtgcagaa acgatttgac gcaagagaag attggtcgta tattcttcca
     3001 acaaaagtcc ctgccctacc gctgctgaag cctgtttatc agcaatttta gtcggacgct
     3061 ttttaaatcc taaggctcca aaaccagccg caatggcacc tgaagacacc aaaatcaact
     3121 catgaccagc ctcgtgcagc atagccaact gctgggtaat atcctttacc ttactacgtg
     3181 ataaacttcc atcctcattt gtcagagaag aagtgcccac cttaaagaca atccgtttgt
     3241 atttcatagt ctcactccga ttattcatat ttatccatta taacatgaat aacaggaaat
     3301 agaaaagagt tgataggaaa aagaaggccc taaaaccttc ttttctacta ctattctgat
     3361 tccgatttgt ttacactcgt ttcagagctt tgtgaagttg aggatgtcaa atcttgacta
     3421 gttgaagaat ttgtaacact ttcatgttga ctcgtttctt tattttcaag atcctttttc
     3481 actacttctt tggattcttt tatttctgaa tgacttgtag ttttaattgt agcagatttc
     3541 tcgatcggaa catccactaa ccaggcacca cttgaatcca cagtatagcc ttctggtgtc
     3601 ttaccattca caagtaattg accattttct ttcaagaagt accatttatc cttgtcttta
     3661 atccaaccta tagctcgtga accacctgtc ttgtagaaat accaatcatt tgccttttta
     3721 agccagcctt gcttcatagc tcctgattct tctagatagt aatgatgtcc agcaacttct
     3781 atctcacctg tcttcatgat accagtagaa tccatatagt accaggcttc tttatctttt
     3841 acccagcccg ttttcatttc ccctgatgga gcaaagtaat accattgccc cttatattga
     3901 agccaacctg tcttcataga gccatcaaca tccaaataat accactgctc tttatcttga
     3961 acccaaccaa tttccatctg attttctttg ttaaaataat accagattcc atcaattttc
     4021 ttccaatttt tagcagaagc accagagtct gtcaaataga accaatgatt gttccatttt
     4081 ttccattgat tctgtaacaa gatacccgtt tggttaaagt aataccattc accttcgatt
     4141 tcattccaac cgacagcata ctctcctgta gaatcaggcg cttgatacca ccagttccca
     4201 tatgcactct tatgccaacc agcttgaaaa cttggaatcg gcttgtagga tgttgaaata
     4261 ttgacaaaac cgtcttttcg aatatcaaaa actgttgcat catagtcttt gctggctgcg
     4321 ttgattctct caattcctcg ttctttgagc caattaacat actcactatc aacaccattt
     4381 ttccaaggta gactatccga agtttgaaca atcaaactcg gactcaaatt tttaatgaaa
     4441 tccttggtat ttgatttgtt ggtatcatgg tgatgattaa acttcatcaa atcaactttt
     4501 ccaatgagag gaccatactt gtcttctgct ccatgaacat tatctaaatc gcccccaagg
     4561 taaattttct tgccattgac tttcaccacg ctaatcaagg aattggaatt gtcatcccaa
     4621 attttcttta attcacccga tgaatcagtt tcattttcat aattatagag ctgaatatcc
     4681 atgtccccaa actgaaaatg agcatcccct tgtgtgatat tttgaataac tgaaacacct
     4741 ttttctgcag cagtctgtaa aaccttatca tagccataca gattatccca tagacgttca
     4801 gaattagtaa tacgactatc actatatttc ttaagataga ctcggtcaac tggataggta
     4861 gacagtaatt catcaacatt tccaatatga tcactgtggg tatgggtcac caaaataaaa
     4921 tcaagttttt ggacacccaa ttccttcaaa cgacgaaaga cacggtctgt tagaacatgc
     4981 ttataagacg tttcaattcc ttctctccat ggataacgag aatcacttcc atctgggaaa
     5041 tcataatctt ctcctgtatc caccatggca aaatgtccat tgctttcaag aataatcgca
     5101 tcactgccac cttcttgaac attgataaag tggattttat ttcctgaact ttcttgagcc
     5161 tgaacattcc cataccatga caaacctaaa aaagcgccta caagtgctaa actagttaat
     5221 ttctttttca ttcttacttt ctcaatctct ccaaggtttc cttaaaaatc tctgggatat
     5281 ctgctttaaa ttccaaggtc ttacctgttc tcggatgagt aaaacctaaa gtcttggcat
     5341 gaagaaattg tccatgtcct ttcaaagtct tgcgaggacc atagacctca tcaccagcga
     5401 ctggatggcc gatataagcc atgtggacac ggatttgatg agtgcgccct gtctccagtt
     5461 gcaactctac taagctataa tcgccaaagc gttccaagac gtgaaaacgc gtcactgcag
     5521 gcttcccttt agcagttaca gcctgtttct tacggtcttt ttcactccgg ccaatcggcg
     5581 cttcaattac accacgatca ttaggcagat ttccatgaac aatcgcccaa tatttgcgga
     5641 gagacttttt atctttgagt tcttgggcaa gtactagatg cgcatcatcg tttttagcaa
     5701 tcatgagaag acctgacgta tccttatcaa tacggtgaac aatccctgga cgcagaaccc
     5761 cattgatacc cgacaagtcc ttaatatgat acatgagggc atttactagg gttccactgg
     5821 tatgaccagc actcgggtgc acaaccattc cctgaggttt gttaacgaca gccacatcct
     5881 catcttggta gactatttct agcggaagat cctcagccac atactctaat acctctggtt
     5941 ctggcacatg gtaagtgacg acatcaccct cttggactgt gtatttagct ttcttgactt
     6001 gaccattgac caagacctgg cctgatttaa tttgttcatt cgcgagacta cgtgataatt
     6061 ctgacaaatc tgacaaagcc ttatccaaac gcagaccacc agtttcaatt ttaatttcca
     6121 tttatttcct cttttagcat tgcaatcaat aaaataatca ctccaaccgt cagatagcta
     6181 tctgccacat tgaaaattgc aaagttgata aagtcaaggt ggaacatatc cacaacaaag
     6241 ccctgactga ccctgtcaat aaagtttcca agaccacccg cgattattag agtcaaaccc
     6301 aagaacatcc agaatgagtc ctccatgtgt ttatgtaaat accaaatggc acctatcacg
     6361 acaaccagag taatgacagc gaataacagc tgctgatctt gtaagataga aaaggctgca
     6421 cctcgatttt gcaggtaggt caagctaacg aaattgggaa tccaggagcg cacttcaccc
     6481 agtggaatct gctggacgat ataggatttg accaactgat ccagcccaat taaaagcagt
     6541 acaatgactg ccactattgc tctttttttc atgatttcct cttttgatca aaatattctt
     6601 gcatgacttc tacgaagaga gtcccagctt gactaagctc cacttcttca cgtttaacat
     6661 agaccatgcg gttatctagg ttatccttga gacgaataac tgtaatgcca ttaacactgt
     6721 cactatctaa aaatccagaa cctgtcgcat aggcgtccgt ccgctccaaa ataccattca
     6781 aggtggcacg gtctgtcaca ttaaacatct gtgagctagc gctggtatcg acaaagttct
     6841 ctgaataata aaggtactcg tctttctctt gagtgaaacg aaccgttggt aaatccgcta
     6901 aatcctccat gactaattcc tctttctggg ctaaaggatg accctcacag agataaatat
     6961 gggtatggaa aggaatcaat tcgatgacct ccagacctaa cttttcaacc cgttgcataa
     7021 tccccttttt attttgattg ttgaggtaga taatcccaat ctcactatgc ccttgcgcca
     7081 cttcatctaa tatttgaaca gtagttgatt caaaaatacg gaagttctta tagtcaggat
     7141 agcgctctga aaaggccgta atagttggtg gtaagaagtc atagtgctgg ctagcaacgg
     7201 aaaattcatc tttttcttct tcaggattgg catactgatt ttgaaaaata tcaaatcctt
     7261 taaccaattc ttgcgctttt tcataaaatt ccatcccacg acgggtcaag aaagtccctg
     7321 agctggtccg acggaaaatc ttaaagccca actctttttc caaatcacga acagaaatag
     7381 acagactcgg ctgactaaca tacatctttt cagcagcttc acgaaaagta ccactattgg
     7441 caatagccac aacatagcgt aattgttgaa tgttcatctt ctacccccaa cttctttatc
     7501 ttttcattat accatatttt agaagttttc caaaaaggaa aaaagaacat cctattcttc
     7561 ttaactatct tcactatctg cctttctcac gccaatctta ttttcaaaat caattcaaaa
     7621 aaataagtgg ctacacacca cttcagtatc ggaaagaaaa acgttgacta tttgtgaaaa
     7681 aagaaaatgc cggaaaattc cgacattttt ttagtaagct aacttcctga aaataggagt
     7741 aaccaaacat cccagaaggc agtgatattg atgagaatat gaactagtat tggatagtag
     7801 agatttttag tcatgcgata caatagagct aaaatcagac ctccactagc ataaatgaaa
     7861 aaagcaagag gagttaaagc aaaattgata tgaatataac cgaaaataat agcagaaagc
     7921 aaaacatctc cgtaccaagg tgagtttttg aaaaaggttg tcataagcac acctcgataa
     7981 atcaattcct cagcaatagg ggcgatgaag caaacgatga gcaagaaata agggaactcc
     8041 tgtctcccca tcatttctac cgtttcattc aaagaaatct gatttgaaga cagggatatg
     8101 aaatacgaaa agaggaagtc agacatatat gaaatgatgt agcccagtaa aaggtagatg
     8161 aagtacctca gctgccactt gaaatgaaaa attttctttc ctgcaaaaac cagtagatag
     8221 atgaccgcta agaaaagaac tccgctctcc atcaaaagca gaatctgaaa aaattcacga
     8281 cttgctggta gatagggctt tgcgaggtga ttgaaaactc tccaaaacca agttgatttg
     8341 taaaaaatta aggacaatgc tagtaaaatt tgaatagccc gttttttcat attaaattct
     8401 ctgctttctc cacttatttc ttcatattac taataaattt tacttaaaat ctcgcagcac
     8461 ttcctttgca aagatgattg cctcctccaa tatatcctaa atcataggtg cctctgggac
     8521 aaaatccatg aaccggcaga gttaatactg gttctatttc ccaatcgtac ctaccattag
     8581 gatttttacc atatgaatct gctccacctt gtatcagctc taattcttca tttgttagat
     8641 ttatattttt tgtcattaaa atcatgatat atttcctcca ttattcttca gattagttga
     8701 gtaatctgat actcatacct acttacaaaa aaactattat attaagttgg ttttttaatt
     8761 tgtctagttg caacattgac aaacatagta tagcatatct ttgcgaaata tcctcttcaa
     8821 atcatgaatt gtcatcaaaa catcttaaac tataaaatca attagtctca agctttctat
     8881 caatttcttc tccaaaatat gctataataa tagcaaaaga taaagaagga agacctatga
     8941 ttaaactact agccttggat atggacggca ccctcctcaa tgaagccaag gaaatcccac
     9001 aagctcacat tactgctatt cacaaagcca ttgaaaaagg tgtcaaactg gttctctgta
     9061 cgggtcgccc ccttttcggt gtcctcccct actacaaaaa actgggactc gacctccaga
     9121 atgagtatgt gattgttaac aacggttgtt caactcacca gactagcgac tggggcttgg
     9181 ttgactggca agaacttagt ccagctgaca tcgaatacct ctatgacctt gctgaaaaga
     9241 gtgatgttca gttgacactt tttgacgagt cacattattt tgttctcggt ggaaagccca
     9301 atcaagttat tgaaaatgat gctaaactag tattttcaga cctgactgaa atttctcttg
     9361 aggaagcgac tagtggaaag ttccggatgt tccaaggtat gtttttagga acaaaagaac
     9421 aaacagacga ttttgagcag cgttttgctg aagagctttg ccaacgattc agtggagttc
     9481 gttcgcagcc tgtcatttat gaagcaatgc cacttggaac gacaaaggct actgctcttt
     9541 cacgactagc tgagattttg aagattgatt cctcagagat tatggccatg ggcgatgcta
     9601 ataacgatat cgaaatgctc cagtttgcag ggcttgggat tgcaatggga aatgccagcg
     9661 attatgtcaa atctcttgcg gatgccgtta cctcaagcaa cgaagaagac ggcgttgcgc
     9721 gtgctattga gaaatatatt ctataaaaaa gaaaagcaaa tagacagaag ttactgctat
     9781 ttgctttttt gctaatattt taaaatacgg actaatcaat agagaagaat agcgaatcat
     9841 aaaaatcgat ttactagatg ccatagtatc ttatagttgc taataagaag ttagtctagc
     9901 agaatgaatc tctcatttct cccacactaa tctgtaattt gtctatacat ttctggtctt
     9961 ctatctcgaa acaagcccca gtttaggcgt tcacttgctc ccttgtctag gttataagta
    10021 gctaacagaa cagcttcttc ttgtctttca gctcgttcta gaatagctcc tgtttcatcc
    10081 gtcataaagg aggaaccgta gaagtcaaga ctggaactct gtccgccatt ttcctcacta
    10141 ggagtaacct cctctaaacc ataacgattg gctgcgatga ctggaacaat attcgctgct
    10201 gcgtgccctt gcatagtacg ttgccagtga ccacaactat ctgtatccaa aattggctct
    10261 gaaccgatag ctgtaggata aaagagcaat tcagcaccat tcaatgcaag acagcgcgct
    10321 gtttcaggga accattgatc ccaacagata ccgataccaa tcttagcata gcgagtattc
    10381 cagaccttga aaccagtgtt accaggcgtg aaatagaatt tttcttgata ataatggtca
    10441 tctggtatat gggtctttcg ataaacgccc agcacttccc catctgcatc aatgacggca
    10501 atagagttat acaagacatt accatctttt tcatagaaac tgattggtaa aacaacttgt
    10561 agttccttag caagcaactt aaaatgctga atggcagtat tttccgctac agattgggca
    10621 tactggtagt agtcatactg acgttcctga cagaaatagg gatgttcaaa caactcgggc
    10681 aagagaataa tttgggctcc ttgctcagca gcctgacgta ctaaacgctc tgcggtttgg
    10741 atatttgttg ccacatcctt agcgcattgc atctgaatgg ttgcaactct tacatttctc
    10801 atctttttct cctattctgg aatttgttgg gtgatacagt ggatattgcc accacctaag
    10861 agaatatctc tggctggtat tccgacaact ttacggtctg ggaaacactt gctgaggata
    10921 tctaaggcca cttggtcgtt tacatcctca aactgtggaa ccaagacagc cttgttggcg
    10981 atataaaagt ttacgtagga agctgctagt cgttcacctg cgtatcgctc ttcttctcct
    11041 tcttcataga tgtagcctgg caaatcttct tctgtcacaa cttgtcgaac tgcagggata
    11101 ggcaatttat gaatggtgaa gtgacaacct tttgcatctg tttcctgttc taagagttcg
    11161 agatctgctt ttgacatggc atactgggga tcattttcgt catctgtcca agccaaaaca
    11221 acctcagcag gaccaacaaa ggcaacaaca ttatcgacgt gttcattggt ttcatcctga
    11281 taaataccat aaggaagcca aataactttt tcagcaccaa gactttctaa taatgtgttt
    11341 tcaatctcct ctttagtcaa gttaggattg cgaccaggac taagcaagca actttcagtt
    11401 acgagaatag ttccttgacc atcgctatgg attgcgcctc cttccagtac aaaaggttta
    11461 gcatcataga caggcctttc caaggcctca gcaaaacgac tggctacttg gtcatcctct
    11521 tcataatctt gataaagacc atcataggtg cctccccaag cattaaaggc ccaatccacg
    11581 gctaatttct tacctttatc attgacgaga atggttgggc cagtatcacg cgcccaggca
    11641 tcattggtgg gaatgtctaa ataaacaacc ttgtctccaa gataggattg ggcttcagat
    11701 agataggcct gctccaccaa aagatagact ctttcccctt ctgctatggt ctcgatgatc
    11761 tgagtaaatg ctcttttagc agcctttcct tgaaaaggcc atgatcctgg tcgagtcggc
    11821 catatcatga gggtaccatg atggggttcg tactctgctg gcatgtgata gcctaatttt
    11881 tttggactgt ccatcatgat aatctccctt taaagtcttg atagccaaaa gctttgagta
    11941 agctacagtc tccctgttcg tccatgagat agagacttgg caatccaata ccattaaagg
    12001 tattattttt gacaaaagaa taaatggcca tgtcttgaaa ataaagtctg tctccgattt
    12061 ggactggatt ttcaaaacta taatcaccaa tcacatcgcc agtcagacag gtattagaag
    12121 aaagtctgta ggtatgggct ttttcctgtg actcaaagcc atttctcaaa ggtggacgat
    12181 agggcatctc aagtacatca ggcatatggc aggtcgcaga ggcgtctaaa accaagattt
    12241 ccataccgtt ttctacaata tctaatacct cagttgctaa ataacccgca ttaagcgcaa
    12301 tggcttcacc aggctcgata tagatttcaa gattgtaagt ttttcggata cgcttgattt
    12361 ctgaaatcag caaatccaca tcgtaacctt ctcttgtaat atgatgacca ccacccatat
    12421 tgagccattt tacctcatgt aagtagggac caaactgttc ttctactgct ttcaaagttg
    12481 tttgtaaatc atctgctccc tgctcgcaaa gggtatgaaa atgaagtccg tcaaccaaat
    12541 ctagcaaatc gctcggaatc ttgtctatag taactccaaa gcgagaacca ggtgcacaag
    12601 ggtcatagag cgcgtgatct ccttgagttg aacactgagg gttgaggcgc aaaccaacac
    12661 tgacaccagc ctctcgacaa cgagccccat gcttacgcaa ctgtctctct gagttaaaga
    12721 cgatatggtc tgttatccca agcaattcct ccatgtcctc atccttgaaa gctggtgcaa
    12781 aaacatggac ttcaccagga aattcttccc ttgccaattt ggcctcatag agtccactag
    12841 ctgtcgtacc tgatagatac tggctaatca agggataagt tttgtagagg gaatatgcct
    12901 tctgggcaag caagaccttg caaccggcct cttcttgtac atattgtaga atgcggcaat
    12961 tagcttctaa cttggccaag tcaataacat aggctggtgt tggtacttgt tctaacttca
    13021 ttagtccaac atttgtggat tttcaaccac aacccatggc aaaccatact cattcaaagc
    13081 ttccatgaat ggatctggat ctaactcctc aaggttatac actccagctt gtttccaagt
    13141 tccgttcatg actaattttg tcccaatcat ggctggaacg cctgtcgtgt atgaaattgc
    13201 ttgtgacccg acttctgcat agcactcttg atggtcgcaa acattgtaga tgtagatagt
    13261 cttttcaacg ccatctttga cacctgtaaa gatacatcca atattggttt tacctacagt
    13321 acgtgggcca aggctagcag gatctggaag caaggctttc aaaaattgaa ttggaacaat
    13381 ttcttggccg ttaaagttaa tggtatccgt acgaaggagt ccaacatttt caagacattt
    13441 catgtgcgtc aagtaagatt gaccaaaagt cataaagaaa cgaatgcgtt tgacacctgg
    13501 aatgttcttg gccaatgatt cgatttcttc atggtgaagg agatacatat ctttttgtcc
    13561 aacttgaggg aaatcatact cacgcttgat agacatagct tcgacttcga cccatttccc
    13621 atcttcccag taagaacctg gcgcagaaac ctcacggaga ttaatttctg ggttgaagtt
    13681 ggttgcaaat ggataaccgt ggtcaccgcc attacagtct aaaatgtcga tataatggat
    13741 ttcatcaaaa tagtgtttga gggcataagc tgaaaagaca ctggttacac ctgggtcaaa
    13801 accagaacca agaagagcag tcaagcctgc ttctttgaat ttctcttgat aagcccactg
    13861 ccatgagtag tcaaagtagg ctgtaaaacc aagttcctta caacgttttt cgtagatagc
    13921 acgccactca gggtcttctg tgtcttctgc ttcgtagttg gctgtatcga tatagtgaac
    13981 acctgttgcc aaacaagcat ccataatggt taaatcttga taaggcagag ctacattcaa
    14041 aacagcttct ggtttgtagc tttcaatcaa ggtaatgacc tcttctacct tgtcagcatc
    14101 aagtgctgca gtttcaattt tagtacttgt tttgccttct agcttcgctt tcaagtcatc
    14161 gcattttgac ttggtacggc tagcaatcat aatctctgta aacgtttcgc tatcttgaca
    14221 aatctttgaa atagcaactt gggcaacgcc cccacaacca ataactagta aacgactcat
    14281 ttttttcctt cctcttcttc taaaatgtcc tcaacatact tgggcaacat aaaggctccc
    14341 acgtgtaagt ttgcagtgta gtattctgtg aaaagctggc gttttttcca gccttccttg
    14401 tcaaaatctt tgacagggtg gtattttttc gatgcaaatc caaacaacca atagccagct
    14461 gggctagttg gaatatgggc ctgataaacc cgactgattg gaaaggcttg attgaccttg
    14521 cggtgcatgc ttcggcaggc cgactcatcc tcgtcaaaga agggactccc atgctggtaa
    14581 atcatgatgc cgtcttcctt cagagctcga taactattgc cgtagaattc cttggtaaag
    14641 agtccttccg tatggccaaa tggatctgtc gcatcgttga tgataatatc gtaatcatct
    14701 tcgcagtttc gcaaaaagcg tagcccattt tggtagtaaa tggtaacacg aggatcatct
    14761 agccctgcag caaagtctgg gaaatactca cgacagacct cgaccaacat ctcatccggt
    14821 tccacgatat cgatttgttc cagttcagga tagagtgtta atacttgggc aacaccgccg
    14881 tcaccacccc caataaccaa tactttcttt ggatttgggt ggacagccat gggaacgtga
    14941 acggtcattt cattgtagac gaaatcatcc gcatctgaga acaagacatg gccatttaaa
    15001 atcagtattt tcccaaaagc tggcgtatcc aagacttcga tatcctgcca ttcacttttt
    15061 ccagcgtaaa gttgcttggc tgttctcaga gacaatttga catctggagt atgaacttca
    15121 gaaaaccata aatccatcta tttctcctta tattat
//
LOCUS       DAVBCG010000039        14857 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBCG010000039 DAVBCG010000000
VERSION     DAVBCG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14857
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.039"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..542)
                     /locus_tag="VQ495_001001"
     CDS             complement(<1..542)
                     /locus_tag="VQ495_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2530974.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLK"
     gene            complement(557..1405)
                     /locus_tag="VQ495_001002"
     CDS             complement(557..1405)
                     /locus_tag="VQ495_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2530975.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            1593..2942
                     /gene="trkA"
                     /locus_tag="VQ495_001003"
     CDS             1593..2942
                     /gene="trkA"
                     /locus_tag="VQ495_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2530976.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            2946..4385
                     /locus_tag="VQ495_001004"
     CDS             2946..4385
                     /locus_tag="VQ495_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2530977.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVVVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(4511..6190)
                     /locus_tag="VQ495_001005"
     CDS             complement(4511..6190)
                     /locus_tag="VQ495_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2530978.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSTFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(6260..7672)
                     /gene="lacG"
                     /locus_tag="VQ495_001006"
     CDS             complement(6260..7672)
                     /gene="lacG"
                     /locus_tag="VQ495_001006"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2530979.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKYNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(7682..8026)
                     /locus_tag="VQ495_001007"
     CDS             complement(7682..8026)
                     /locus_tag="VQ495_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2530980.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(8194..10080)
                     /locus_tag="VQ495_001008"
     CDS             complement(8194..10080)
                     /locus_tag="VQ495_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2530981.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYMRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFFALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(10082..11404)
                     /locus_tag="VQ495_001009"
     CDS             complement(10082..11404)
                     /locus_tag="VQ495_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2530982.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11572..12795
                     /locus_tag="VQ495_001010"
     CDS             11572..12795
                     /locus_tag="VQ495_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2530983.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            13094..13264
                     /locus_tag="VQ495_001011"
     CDS             13094..13264
                     /locus_tag="VQ495_001011"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2530984.1"
                     /translation="MTKQIPKFTKDTAQLYTCKWLLYNKVTKMYDHTIVNHSVREYIT
                     DSISTNTIEEAE"
     gene            13876..14160
                     /locus_tag="VQ495_001012"
     CDS             13876..14160
                     /locus_tag="VQ495_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530985.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            14153..14391
                     /locus_tag="VQ495_001013"
                     /pseudo
     CDS             14153..14391
                     /locus_tag="VQ495_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14487..14705
                     /locus_tag="VQ495_001014"
     CDS             14487..14705
                     /locus_tag="VQ495_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2530986.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT         4887 a         3072 c         2378 g         4520 t
ORIGIN      
        1 tttttcaaac ttcctgcacg agtttgggtt cctgcatagg caagtaagag aagagttcct
       61 gcaatagcta cagatacacc attggcaatt cccgcaacaa ttccttgtgc aaatactttt
      121 tctgccgctt cttgataaat cacaacatct ccaagtggtg ccaagacacc ccaaacaagg
      181 gcatttgcaa gtagttgaat gaggttaaaa ataagaatat ctttccagtc aaaaacacca
      241 ttgatcacgc gaacgtactt tctaaaaagt cccacaacta aaccaaagag tccgctagcg
      301 ataatccaag tccaccatag accataacca acaagagagt ccttgattgc atgaccaatc
      361 aacccaacaa gcaaaccgat aatcggtcca aaaataatag aaagtagcgc ttgtaccgca
      421 tactgaagct ggatgcttgt atttggaaca ggggttggaa tgttgatcat cccgatgacg
      481 acaaagaggg cagcgccaat tccgacagca acaacttgtt taattgtaaa tttgatttcc
      541 atactattct cctattttat ccttctattt tctttatttc aatggtccaa gatgaaccga
      601 cacctacatt ataggccttg gcaaaggaac cttggttgat agccaaacct aaacgataga
      661 gagagttgat gtaaaggatg ggttgcccaa ttctcacatc tgcaaatgat ttgccataga
      721 caacctgatt ttgatagacc agcatatcag catgatagat ggtcacttca aaacgatcac
      781 caaattctgg ttccaacttg taaaattctt cccgtgtgat agaggtccaa agcgaaccga
      841 aacgcacatc cagaatatca atggctccct tcaccagatg atcttctatg atggtcgcta
      901 cgactggaag ctctacaatc tgttccacac tgagctctgg ccctacttcc tcaaaagtaa
      961 tgtgaccact ggccagttta gcaccagtat aggcatagac atcacgaccg tggaaggtat
     1021 aagaatgctc tgtgttttga cgcctattgg ccacctcaga aatctcacga atggctacaa
     1081 tgccaacgtg tttcttgata aaggaaagcg tcccattatc tggcgtgaca atgtattgat
     1141 tttttgcagt cttggcaact acactcttac gtttcgaacc gacacctgga tcgacaaccg
     1201 atacaaacgt cgttccctca ggccagtaat ccaccgtctg aaagagacga tagctcccct
     1261 caaaaatatt ataaggcgtg atatcgtgcg tcaagtgatg gatttttaag gttggagact
     1321 cttctaaagc cactccaatc atagccgata ccgcaccatc aaccagacca aagtctgatt
     1381 gtaataccag taaattatta ttcattttat ctccttgtat atcctttatc ataccatatt
     1441 tcagagaaag tcgctaatag ctatttcaat tggttagggt atagttttat cttatagcga
     1501 attttcacta aaagctcttg ttattagcta gtaggtgcat tttttcttga aaaagggtat
     1561 gatagttaca tcatgaaaaa gtaggtttta ttatgaaaat tatccttgtc ggagggggaa
     1621 aagttggttt tgccctctgt cgctccttgg ttgcagaaaa gcatgatgtt ttgctgattg
     1681 agcaagacga agctgttctc aatcatattg tcagtcgctt tgatatcatt ggtatccttg
     1741 gtaacggggc cgattttgcc attcttgagc aagccagcgt ccaagattgt gatatcttta
     1801 tcgccctgac tgagcacgat gaagtcaaca tgattgcagc agttctagcc aagaaaatgg
     1861 gagctaaaga aactatcgtt cgggtgcgga accctgaata ttctaactct tatttcaagg
     1921 aaaagaatat tctcggtttt tctcttatcg ttaatcctga gctcttggct gcccgtgcta
     1981 tcgcgaatat cattgacttc cccaacgccc tgtctgtcga acgctttgct ggtggacgcg
     2041 ttagcctcat ggaatttgtc gtcaagtcca ccagcggtct ttgccaaatg cccatttctg
     2101 attttcgtaa aaaatttggt aatgtcattg tctgtgcgat agagagggat catcaaatta
     2161 tcattccaag cggtgacatg actgtacagg ataaagatag aatctttgtc actggtaacc
     2221 gtgtcgatat gatactcttc cataattatt ttaaatcacg cgccgtgaag agccttctca
     2281 tcgttggggc aggtagaatt gcctattatc tacttggtat tctcaaagat agtcgtatcg
     2341 atacaaaagt cattgaaatc aatcctgaaa tcgccagctt ctttagcgag aaattcccaa
     2401 atctctacat cgttcaagga gatggaaccg caaaagatat cctgctggaa gaaagtgctc
     2461 aacactatga tgccgttgcg actctaacag gagtcgatga ggaaaatctg attacatcta
     2521 tgttccttga cagggtaggt gtacagaaaa atattactaa ggtcaatcgt accagtctcc
     2581 tcgagattat caatgcgcct gatttttcaa gtatcatcac acctaaaagc atcgctgtag
     2641 atacgattat gcactttatt cgtggtcgag ttaatgccca gtattcagac cttcaagcca
     2701 tgcaccatct agccaatggc caaatcgaaa ccctgcaatt ccatatcaag gaagccaata
     2761 aaatgactgc caaacctctt tctcaactga aattgaaaaa aggggttctt attgcagcca
     2821 ttattcgaaa gggcaagact attttcccaa ctggggagga tatgttggaa gttggagaca
     2881 agctcctagt aacaaccttg ttgccaaaca tcaccaagat ttatgacttg attgcgaggt
     2941 aagaaatgaa taaaagtatg attcgttacc tcctttcaaa gttacttttg attgaagctg
     3001 ttctcctctt ggttcctgtg gctatcgctg tctattaccg tgaatcgagc caagtcttta
     3061 cagccctctt ttcgaccata gggattctcg tattgttagg cggttcaggg agtttacaga
     3121 agccaaaaaa tcaacggatt tatgccaagg agggagtctt gatcgttgcc ctctgttgga
     3181 tcctttggtc tttctttggt ggtctcccct ttgtcttttc tgggcaaatc cccagcctta
     3241 ttgatgcctt ttttgagatt agttccggct ttacgactac tggagcaagt attttgaacg
     3301 acgtttcagt tctcagtcgt tccctcctct tctggcgaag ttttacccac ttgattggag
     3361 ggatgggagt gcttgttttt gcacttgcta ttatggacaa tgccaaaaat agccacctag
     3421 aggtgatgaa ggctgaggtt ccaggtcctg tttttggcaa ggttgtatcc aaactaaaaa
     3481 acactgccca gattctctat ctcctttatc tagctctctt ctccctcttt gtcatcatct
     3541 attatctagc tggtatgcct ctctacgata gttttgtcat tgctatgggg acagcgggaa
     3601 ctggaggctt taccgtctat aacgacggaa ttgcccacta tggcagctca ctgattacct
     3661 atctggtcag tatcggagtt ctggtttttg gagtaaattt caacctctac tactacctca
     3721 tgctccgtcg catcaaggcc ttctttggag atgaagagct tagggcttac ttggtcattg
     3781 tagtggtttc tacaggcttg attagcctca acaccctcta cctctaccca gggttttcaa
     3841 agagctttga aatgaccttc ttccaggttt ccaacatcat tacaacaact ggttttggat
     3901 atggagatat taccaactgg cccctcttct cccagtttat cctccttttc ctcatggcaa
     3961 tcggtggctc tgctggatca actgcaggtg gactcaagat tattcgaggc ctcatccttt
     4021 caaaaattgc caaaaaccaa attttgtcaa tcctatcgcc ccaccgtgtt ttgaccctcc
     4081 atgttaataa aacggtgatt gacaaagata cccagcataa gattctcaag tactttgtca
     4141 tctatgctat gattttgcta gcgcttatct ttattgtcag cctagatagc aatgattttc
     4201 tggtcgtgac cagcgctgtc tttagctgtt tcaataatat cgggcctatt ctaggaacca
     4261 cttctagttt ctcaatcttt agtcccatct caaaaattct cctatccttt gcaatgattg
     4321 cagggcgctt ggagatttac ccaatcctcc ttctctttat gaagagaact tggtctaaga
     4381 gataaaatac aaaatccctc tttttacaat tgtgaaagag ggattcttat attaaaaaag
     4441 ggggaacgtt tttaaatcca agcctatctg taagcaaaat ccttactcaa ataaaagatg
     4501 gaaaaactta ctacacagct tggtaatgct ccaatacaaa ttgtaaggat ttacttggac
     4561 tctttgttaa atgaatatat tccattcctc tggtagcaac tatctgaata cctaatcttt
     4621 ctgcatccac cttcatctct ctataataac tccgaacttg tggagccaga ataattaaat
     4681 cataaacacc cataatatca taatgagctc cgtacgctcc tgaattcgca atcactctaa
     4741 cttctgctaa ttgagcgccc tcgttaattg cattggctaa ttgcgcactt gttccagacc
     4801 ctgcacaaag aaccagtact ttcaactcct tactttttat ttcatctaac tcaccaggca
     4861 ttatttcact tgtctcctcc tctaaaataa tatcatttga gcttgcaaca tcttctttca
     4921 tcagtaactg tctatcatac gctctacaga atggcaaata aatcaatatg tcgacaacta
     4981 aaatcaaaga taaaaataca aaggagatag attgaaaatt cgttccaatt aacaatccca
     5041 agggaccagg aaaggcccaa ggtaactgga tataaaatcc attcattcca aagaaatcaa
     5101 taaagacctt tcctagaaat acattcactg gtggagtcat caaaaaaggt acaaaaagat
     5161 agggattcaa aataactggc ataccaaata gaagaggttc attcaccgca aataaaacag
     5221 gagtaattgt agctttacct accgatttta attgtttaga ccgcataaag aaaatcaaaa
     5281 taaatggtac aacaaaggta gcccccgttc ctccgatagc agcgatataa tttccgaaat
     5341 taggcgttaa agaatgataa ggaaactgcc catttgcaag aagttgagca ttctcttcca
     5401 tattgctcag ttgtaaagct gtaacagcag gtaagacaat agatggtcca tgaattccaa
     5461 caaaccaaag caaagcgcac ataaaccaaa tcaacatcat agcaggatat gattctgccc
     5521 ccttaaaaat aggagtcaat agttgttgaa atacttgggc aaaaggaaca tctaaactaa
     5581 accgagatac aatatctaac aaaccactaa taagtaaaac aaaagaaaaa ggaaaaatat
     5641 ctctaaaagc ttgtgatata gccccaggaa cttccttagg taaatgaata gtaatgtctc
     5701 gcttaataca gaatcggtaa acatttacag taataaaggt actgacaaac gaagttatca
     5761 atccttgaga tcctaaatag tttgtcgaca aacttgtaga tcccgttttc tcatcaacta
     5821 caagcgttac agttaataca agatagccac atatggccgc caacattgca gaagtatcat
     5881 ttattacctt tccaggaggc atttttctat taacatttcc agccaatgac tttgcaactg
     5941 tccctgaaac aataacaccc aaaactccca tggttagcat atacactttc ctcatccagg
     6001 caatagctgg actcggtaac tctactccga aatttttagg aatcataata ataatcatca
     6061 aaaagcttga aaacataatt aaaggcatag tagcaataaa tccatcttta atagccatca
     6121 tataattatt agtagaaatt ttttcaaatt tcccttgttg cttttcaatg gcagcgacta
     6181 acttatccat aaaaactcct cctgtaaaaa atttaagctc atagttaaaa tatattaaac
     6241 atcagtcaaa tcgctaacct taattccgtt ttaatccttt tacccaaaga gcacttttct
     6301 taatatatct cttctgtgtt tcataatcaa caaagaaaag accatatcgc ttattgtagc
     6361 cattcgccca agaaaattga tcttgcaaag accatataaa atagccttga atattgactc
     6421 ccctatctcg agcctctaaa actttgtgta aatgttgctc aatataacgg attcttttac
     6481 tgtcatcaat aatatcattc tcgccctctg gcttaacctc tttaagggct gtaccatttt
     6541 cagttaaata aatgaccgga tgttgaggat attcttcttt gatacgaagc aacatatcaa
     6601 ataacccttg aggataaata ttccaatccc aatctgttgt cggaatacca ggctttttta
     6661 caaattcacc tagagcattt aatttaaaag aagactgtcc ttttattcct gttgaattaa
     6721 attcttgtct attttcacct ttgtattcac gaataaattc tgaacgataa taattcatcc
     6781 caaacatcgt attaagaggt gcagctcttt tcataatttc taaatcacca tcttcaataa
     6841 taaagctaga attattagct tccaatattt gattcaagtg aaaaagcgtg tcatcactgt
     6901 agtagcccaa aaaagttcca tctaatagaa atttattatt ataaacatca taccgtttag
     6961 ctgccaaaat attttctttt tgcccatcaa taggatagcc tggctttaaa gcatgaatac
     7021 aacctacctt tccctcaatc cctaattgat gaaattcgag gactgcaaga gcatgcgcca
     7081 acaacatgtt atgatttgct tgaattgctt cagataattg aaaatggtga ttcggaggaa
     7141 actgacctcc tatgtattgg cctgcagcaa gagacatcag ttcattgatt gtaaaccaat
     7201 gcttgacttc tgtaaattct tggaaacaaa agcgagcata tcgtatgaaa cgatcaatat
     7261 tctctctatt caaccaatcc cctgtttcta acattttctg aggcgaatca aaatgatgta
     7321 aagaaacaaa cggaatcaca ttatatttta agcaggactg aaagactcta tggtaataac
     7381 gaacagccaa tatattcggc tccccatcta tatcaggaaa tatacgaacc caagaaatag
     7441 ataaacgcaa agcctgcaaa ccatgttctg ccgccaaagc tatatcctct tcgtaacgat
     7501 aataaaaatc actagctgga tctggtaaga acggactatt ttcttgcaaa taaacatccc
     7561 acatatttat tcctttgcca tctaccctag ttgcaccctc tacttgataa gcagcggtag
     7621 cagctcctaa taaaaactcc ttcggtaagg ctttaacctg taaattttcc atgactaatc
     7681 ctcattgcaa accaatatgt tcttcaatct ttcgaattcg ttcatattca tcaataaaaa
     7741 actttacctg atcatacaat agcatcgttg tcatcaaagt atcttgacca tgaatcatga
     7801 taaaactcac ttcaaaatta tctccagatg cctcctccgc taataaatta gtctgttctc
     7861 gatgggcaga cactattgag tcgtttgcag actcaaccaa ttctcttgcc tgttcaaaat
     7921 atccatctct cgcaaacgcc aaagcatcca tcaaatctga ccttgcatct cctgcgtaag
     7981 cgacaattgt aaaaccaagc atatttattt cttcttttga tttcatcttt cttcctcctc
     8041 aatcttgaac catcttttac acatcattat taaaatgact tcgctacgat acaaatagaa
     8101 agtcgaccct aaatatctta aaaatcctta aatttcataa tatttaggat caatcttctc
     8161 tgaatacctt ggttcatttt ctagacttat gatttataag tggaaaccag cgtaattttt
     8221 tgctacttca attacacgat aaacatccaa attagattgg aatagcagtt cgtgcccacg
     8281 gatataattc aacacatttc tccattcatc ttgagcttgc cttgtttttc cagttgcttg
     8341 aagatataaa gccttcgcca tcttaacaac atattcacga tgataactga gctgtttcca
     8401 ttcatccttt tgactattta ataacttaga aatattttcc tcaataattg gtaaaaaatt
     8461 atcagctaga ttgtaagcta tataataatg attcgctaaa acatcatttt gacgactgcc
     8521 aattgcatta aaatagtcac aagaggaata aatggataat ttttctaaat attcaacaac
     8581 agactgccaa ttttccccat acaaagcaaa aaagtattct tcaatcaatt cttcataact
     8641 tcttgtcttc ttccagagca tttgccccat gacataatta ggaaaattat gagggaatcc
     8701 ggcacgtaat tcttgacacg aaatgtaccc attcaaatgt aggtttgaaa gataagatac
     8761 atctctgtaa atagtttgac taattttcat atagcctaaa tcgccataat gagcacgccc
     8821 taaaggatag tcatatacga aactatctcc tttaaatgtt ttttgccact caaaaagata
     8881 agataaattt tcctcaagag aattcggaag tataatttta ttacgcatat aaggtttagg
     8941 cgtaggtatg gaattgtcaa aatctacatc tgcataactc atttcaaatg ttcttgtaat
     9001 cggtgcaaac atcatggtaa agcgttcagg attatctaat ttttctttct gaggcgccca
     9061 taacaactca tggtaaagca gaaaacaaat ctttgtatct aatccctcac tcgttaaagc
     9121 cctatccaat tgattgagaa tacgaatata ctgatccgaa accaattctt gtctacagtt
     9181 ttcgcattca caaatattat tacgagcatc cgacaaccat acatgtaagt agttaacatc
     9241 aggtcttttc ttggcataat ccttgataat ttctaccatc ttatcagcta catctggatt
     9301 tgaaaaatcc aggctggtta aaatcggagc cgtattaaac aattctcgtt tcccgtttat
     9361 ttcagcgaca tagggtttct tctcctctga aatactaaga cctgattccc agccaaattt
     9421 tgaagagtaa cctaaaacct cacctgtcca tccatgacca acacgatgat gaataagacc
     9481 tcttttttgc aattctttat ccaacctatc actcaattct tgtactaatt catttgagaa
     9541 tttttcttta tttaggtatg gattaaactc atgttcatac caacgtttca aaaaagagta
     9601 aggattttca aactggatga aaaaactgtt catcccaatc ttaggtagcc aatcaatgaa
     9661 atctagtata ttttcaaatg aatccgctcc ctctatacat actcccctat gatagtaact
     9721 ggctgtttca tctatagata gctgtttatc taaaaaatct tcaaatcgta actctggaac
     9781 aaagtcatgt ctgcgccctg gtctagtata cacaacccca aattcatgaa acattcggta
     9841 aattccaata agtaaaccta caattgtatt agactcaatt ctccccttac ccttgtctaa
     9901 tgagataaga acatggtctt ttctgtcctc tttattgact aatcctacaa aaatatccgc
     9961 ttccttctcc tcgacaagct ctatgttagt cttaaagacc cgattcatat agtaacttaa
    10021 ttcctctaag gcaaagctag aataagtatt tttctgttga tcaaatactc tgataaccat
    10081 cttatttccc tccaacagta gctttttctt tttcaagatt gcgtttatct gcaattttga
    10141 aaaatggata ataaattgct acagaaacca aaatcaaaac aacttgtaat agagctcctc
    10201 gccaactagc tcctgtcgca aggaagcctc ctatgatcgg tggcattgtc cacggaagga
    10261 ttacacctgt tgtatagggt actaatccta cagccattga tacataggta atcaaggcat
    10321 tgattgtagg agtagcaata aacggaatca gcataatcgg atttaaaact gttggaaacg
    10381 taaatagaat agctgtattg atattaaaaa tagacggtat aatagctagc ttacctaatg
    10441 ttttattcgc acgactctta ctaaatagga agagacaaat cgcaagacca atagtcgctc
    10501 caccgccacc aataaataca aataaatctt taaacggtaa tgtaataatg tgttggagtg
    10561 tttgtcctgc agccacagct tcttggtttt ctgtagtaaa ttgtaaccaa actgggtcta
    10621 caaaagcatt taaaacttgt cccccattaa ctccacagaa ccaaaagaat gagtttacaa
    10681 taacacatag aatcatacct ggaagcgttc ctgcaattaa cttaagcggt gttccaacaa
    10741 ttgcacctag gagtccgttg agacctcctg caactcctgc cgcttcaaga ccttttaaga
    10801 ccagagccca caaaacaaaa gtagtaaagc caggtaaaag agctgaaaat gatttactta
    10861 ccacatctgg gacaccactt ggcattttta tcgtaattcc gcgctggata aacatacgat
    10921 aaatctctgc tgtaaccaat ccaatagtaa tcgccataaa caaagaagat gcattcaggc
    10981 tagaaaatgg tattgcgccg ccaaataact gcttgacctg ctccccattt ttatcataaa
    11041 ccatactcga aaaacgaggt gccatcaata caaaactgga taaggctatg atccctgccg
    11101 acggaccatc tgtaccatat ccttccgaca acctgtaggc aataccgaaa caagcgacca
    11161 aggagataat cgctactgtt gactgattca tagttgtcaa gatacttagc aatccaacac
    11221 tattcagcca atttgtaaaa gcctcttgag gccaactgat tagaataaga aataaagagc
    11281 cgactaaaat caaaggcatt gccatcataa atccttcacg aatagctttc aaatgccgtt
    11341 ggttgccaac tttttcacca aacggagcga cttttgtttc taaaaacgct aaaatattgt
    11401 tcatcaccgg aactcatttt tttatttttt ataaattaaa tataccacta acagaaagcg
    11461 ttgtcaatat attttttgtt gtttttacaa tatttttttc tttgtataga ataataattt
    11521 taattgattt tttcttatgt ttaatataaa ataaaccaaa ggaggaaaca aatgattaca
    11581 aaacaatcag cctttttaca aaatagagcc actatactgg agtttctata tcgaaatcca
    11641 gcaacatccc gtacagatat tgttaatgaa acaggactta ctccagcaac aacgactaac
    11701 atcataaaag agctaagcga acagtccctc atatacgaaa ctggtgatga atttagcgag
    11761 ttttccggat ctggaagacg tagaaaaact atttctatca cagataatat tccgtatgtt
    11821 gttggaggta ttgaaataaa cgtcctaggt atctttctca gtctatgtga cttacaaggg
    11881 aaaactcttt tcgagacaga aattttgaat gaagattatc ctatttcaga aatcaattcc
    11941 accattacca atatgataaa aacagctata gagtacgtcc ctttggaaac aaaattactt
    12001 ggatttggct tatcaatacc tggacattat aacaaagact ccggaagtat cattacaaac
    12061 aaccccatat gggaatcttt taatttatta aatgtaatta aaagattcaa ttttcctttt
    12121 attgtaaaaa ataatatcga ttgtatggct ataggacaat acctttttaa tccacacaat
    12181 acccccgata actttatttt cctacacgct ggattaggta tttacacttc ctttttcaca
    12241 aaagaaaaaa taggagcctc taaaaatcct tatatcggag aaattggaca caccattgtc
    12301 gaattgaatg ggcaatattg tgaatgcgga aaaaaaggtt gtttacaaac atatatttcg
    12361 gatgcttggt taatcaaaca tgcccaatta ttatttaaaa attctcaact aactgtacta
    12421 aaaagccttg taaagactga aaaagacatt catttagata cccttttaac agcttacaac
    12481 ttaggcgact ctgctttacg ccaacaaatt gataaaggag tcaatttatt ggccacttct
    12541 attgcaaatc ttctcctcat caatcctgct gataaaatct atatcaacag tcaattgctt
    12601 aattatcaac ctttcactca tgaagtcagg gataaaatcc aagatcagct ccacttcgtt
    12661 ccctttactc gtaatataga aattgaaatt ttaccttaca acaaacatcg tggaagtata
    12721 ggagcttgtg cattagctat cgttgctttt ttcatagagc atagcaatgt attacaagat
    12781 attatttcac cttaatatat attagaaatc tatagacctg tttaaatcaa ctataacctg
    12841 tagtaaatat ctcgtattta gacaatatga aaacaagacg acttccatat aggaaaccgc
    12901 cttctcgcta tgctgcatga aactgtaaaa atagatgaaa ttgatagcgg tagtcagatc
    12961 aagaaacatc gtaactctaa aaagtcacaa tatataagtt catcctcgga aaaatatcat
    13021 tctaattatt gaaatgccta catgaaaaga aacgttaaat gctcataaaa caacgaatac
    13081 aggtatcaaa actatgacaa aacaaatccc taaatttact aaagacactg ctcaacttta
    13141 cacctgtaaa tggttgttgt ataataaagt tacaaagatg tacgaccaca ctattgtaaa
    13201 tcatagtgtt cgcgaatata ttactgatag catttctaca aatacaattg aagaggctga
    13261 ataggcttta aacgaaatat gtcagtgttt tggcattcct agccttcata tcatttaaag
    13321 aattgtatag ataaaatttt ttccaataca gacactcgta acaactgctt catttttcta
    13381 ccaacatatt taggaacagg ataagataca agaggattaa tccatagctc agttctatac
    13441 caatctaaga caaataagct aaaaaaacga ttgataataa gcaaatagat tccaaatttt
    13501 ctctatctgc tcattttaat aaacaatact agtgtaacta tccttccagt cagaagcttg
    13561 tcaaatcaca ccgaaaattc ttctaaaatt tatctcgtta ggcaatcaag caaaaactcg
    13621 acgatagtac aaacattatc atacagaatt gacttcctaa attatatact ttagtaaggt
    13681 tttcggataa gaaaaaaggt tcattttaca tttctaaaca ttcttttcta agatgaaaaa
    13741 cagaattttt cgattgtaat ttaaagcaac aagaagattt tcagtatcat cctatagata
    13801 cgagctaatt aagaaaaact acatttttga atataaacta caataatata aactaaattc
    13861 cataggagga agacaatgga ttggtacgat tatatgatac aggcatccaa acaatcacaa
    13921 ttcaacgcaa gccattggtt tcgctatttg cgaaaagtta tttttgaaga ctattcttat
    13981 ttaacaaacc aagatgtaga aaagttgcta gactccaaag aactaacccg ttttcaaaaa
    14041 attagcttga agtatgcctt tcaagagcat actccaactc ataaatatgt gatttcatta
    14101 aataaacctg ctaagttaac caatgttcaa aaattgatgg agaaatacaa acatggataa
    14161 aatgaaaccg gtcttccaag ccctaaataa gaaattaatt caggaaaatc tgactttaac
    14221 aattatggtt atgtcttaga atatcatggt ttacgtgcca cacaagatgt tgatgctttt
    14281 tatgaccaaa atcagaaaat caatgaaatt attgctcgtg taggaaaaca gtttaatctt
    14341 aacatccacg aagagttatg gctcaacaat catgttgcaa agcaaatatg aacaaacaac
    14401 ctcccttaag tttatgcgaa tccttgtact catttgagaa tctgaccgtt ttagtagtcc
    14461 ctattgagta tgtgttagga atgaagatga tgagtatcag agagcaggat ttgaaagata
    14521 ttggtgcaat tattaaatac aaaaactttc attcaccatt cgataccttt aaatatttaa
    14581 aagacatggg atttgatact attgacctct ctgttctatt agaaggtttt agttatgctt
    14641 atggtatgga ttggttagaa aaatttttta aagaaaatca agataaactt cgcgaatttt
    14701 attaacagta tcaaacaaga tgattgtcaa ttagccagtc gtcttgtttt tatgatggaa
    14761 tgaaataaaa tcggaacaaa tcacttagag aattccagac aatttctagc actgtttgag
    14821 acgtttcagc ggactatagc aaaatgaaat aagaata
//
LOCUS       DAVBCG010000040        14297 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBCG010000040 DAVBCG010000000
VERSION     DAVBCG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.040"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            120..902
                     /gene="thiM"
                     /locus_tag="VQ495_001015"
     CDS             120..902
                     /gene="thiM"
                     /locus_tag="VQ495_001015"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2530987.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            904..1533
                     /locus_tag="VQ495_001016"
     CDS             904..1533
                     /locus_tag="VQ495_001016"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2530988.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      1742..1830
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2092..2652
                     /locus_tag="VQ495_001017"
     CDS             2092..2652
                     /locus_tag="VQ495_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2530989.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            2653..4038
                     /locus_tag="VQ495_001018"
     CDS             2653..4038
                     /locus_tag="VQ495_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2530990.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            4040..4690
                     /locus_tag="VQ495_001019"
     CDS             4040..4690
                     /locus_tag="VQ495_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2530991.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAIFPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAYGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            4701..5393
                     /gene="tenA"
                     /locus_tag="VQ495_001020"
     CDS             4701..5393
                     /gene="tenA"
                     /locus_tag="VQ495_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2530992.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            5398..5922
                     /gene="thiW"
                     /locus_tag="VQ495_001021"
     CDS             5398..5922
                     /gene="thiW"
                     /locus_tag="VQ495_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2530993.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            5922..6725
                     /locus_tag="VQ495_001022"
     CDS             5922..6725
                     /locus_tag="VQ495_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2530994.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            6718..7350
                     /locus_tag="VQ495_001023"
     CDS             6718..7350
                     /locus_tag="VQ495_001023"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2530995.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7559..8350)
                     /gene="thiD"
                     /locus_tag="VQ495_001024"
     CDS             complement(7559..8350)
                     /gene="thiD"
                     /locus_tag="VQ495_001024"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2530996.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(8434..8532)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8716..9111
                     /locus_tag="VQ495_001025"
     CDS             8716..9111
                     /locus_tag="VQ495_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2530997.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            9122..9493
                     /locus_tag="VQ495_001026"
     CDS             9122..9493
                     /locus_tag="VQ495_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2530998.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            9503..11746
                     /locus_tag="VQ495_001027"
     CDS             9503..11746
                     /locus_tag="VQ495_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2530999.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            11953..13728
                     /gene="spxB"
                     /locus_tag="VQ495_001028"
     CDS             11953..13728
                     /gene="spxB"
                     /locus_tag="VQ495_001028"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2531000.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            13839..14186
                     /locus_tag="VQ495_001029"
     CDS             13839..14186
                     /locus_tag="VQ495_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2531001.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT         4223 a         2606 c         3175 g         4293 t
ORIGIN      
        1 ttgctctttg attttcattg agtataaatc attcaatgta aatgaaaaaa attcttaacg
       61 aaagatagaa ataaatcgaa atcagtagat cgtataaagt gaaaaggaag gatttcaata
      121 tgacaagttt aaaattatta aaagaaaaag caccattggt catttgtata accaatgatg
      181 tagtaaaaaa tttcacagca aatggattag tagcactggg cgcctcacca gccatgagtg
      241 agtttccagc agatttagag gatttgttaa agtatgctgg tggtttatta ataaacatag
      301 gaacattgac agatgaaaat tggaaattat accaagctgc tctgaaaatt gcagagaaat
      361 ataatgtccc agcagtttta gatcctgtag cctgtggagc aggagaatat agaaaaaaag
      421 tagcagatga tctaatcaac aattataaac tagcagcgat tagaggaaat gctggcgaga
      481 ttgcctcttt agtaggaata gatgtggcat ctaaaggagt agatagtgcg ggcgtagata
      541 atattgacga aattgctcta gcagcaaatg agaagttcaa tattccaata gtagtaacag
      601 gtgaagtgga tgccattgct gttaatggag aagtggtaat gattcataat ggtagtgcta
      661 tgatgccgaa agtcattggg acaggatgct tattaggagc tgtagtagca agctttatcg
      721 gactagaaaa aggtcaagaa ttgaaatcat tagaaacagc aatgttggtt tacaatatcg
      781 ctggagaaat ggcagaaaaa cgtccaaatg gacatcttcc tgggacattt aaagttgaat
      841 ttataaatgc cttatacgag attacagatg aagatgtaaa ggaattcaaa agagtgaagt
      901 aagatgtttc ataaagaatt actaaaacta tattttattt gtggaacgac tacttgccaa
      961 ggaaaaaatc tatatacagt cgttgaggaa gccttaaaag gtggtataac cttatttcaa
     1021 tttcgagaaa aaggtgaggg tgctttagaa ggcttagaaa aacttgagct agctatccaa
     1081 attaaagaac tttgtaaaaa atacaatgtt ccatttatcg ttaatgacga tatagattta
     1141 gcaatggaaa ttgacgccga tggcgtacat gtaggtcaag atgatattgg tgttgatgaa
     1201 attagaaaat tgatgccaga taaaataatt ggtctttcta taagaaacga agaagaattt
     1261 caacaatcaa aagttgaata tgtagattat gtaggtgttg ggcctgtatt tggtacccag
     1321 tcaaaggatg atgctggtgg tactataggt tatgaaggtc ttgaattgat gagaaaacta
     1381 ttgccacaaa tgcctttagt tgcaattggt gggatacaga cgaaacatat taaagacatt
     1441 ataaagacca atatggatgg cgtttcaatc atttcggcaa tatcgtatgc aaaaaatata
     1501 gaaaaaactg ttcgggaaat gagtgaacaa tagatatcta tccttggatt ctaagtaagt
     1561 aggattttcc ttttttaaaa agctatgtcc agattcagct tgaatggcta attcatcaaa
     1621 caggtcattt gttttttgca aaagaaagat ctgctgtctt tgtgagtgca aggctgtatt
     1681 tccagttcaa tttcttgaac tagtataaaa tgtgtgttat aataatcaca taattaaata
     1741 ataaagacat ttggggtgct ttaggctgag atgataccca ttgaacctga tacagttaag
     1801 actggcgaag ggaaatgtga aatgtttttt tcgtatgtcg aaaagaactg tctaatcttg
     1861 taagctgaac tctaatttgt atctgtaatt agagtttttt cttttacgaa actggatgaa
     1921 gatgatatag tagattgaaa taagatgcga acaaatcaat tagaaaattc aaattaattt
     1981 ctagaaatat tttagtgttc ttgtgtactg ttctaaattc agtctgctat acaaagtatt
     2041 taggtcggtc ttcttgagtg ccccactttt ctttaaaaaa aggagttttt tatgttgaaa
     2101 aaatggcagt taaaagatgt tatcttgctt gctttcttgt ctatcttttt tggtggggtt
     2161 ttcgttggtt caggatatgt gtataatatt ctcagtctac tcttaacacc tcttggtttg
     2221 caggcctttg ccaatgaaat cctcttcggt ctctggtgta tggctgcgcc cattgctgcc
     2281 atctttgttc cgagagtcgg aagtgcaacg attggagaag tgctagctgc gcttgctgaa
     2341 gtcctttatg gtagccaatt tggtctagga gctcttttgt ctggctttgt tcaaggtttg
     2401 ggaagtgaat ttggttttat cgtaactaag aatcgctatg aaagttggct ctctctaact
     2461 gctaatagta ttgggattac gcttgttagc tttgtctatg aatacattaa gttaggttac
     2521 tacgcctttt cccttccgtt tgtcctttcc ttgcttgtgg tacgttttat ttctgtttat
     2581 ttcttctgta ccatcttggt tcgtgccatt gtcaaactct atcatcagtt tgcaactgga
     2641 ggaaaagcat agatgggtct ggaactacga gcgattcagt ccccaatctt ctctgagccg
     2701 tttgatttta cttttcatgc gcaagccttt accttgttag ttgggagtag tgggtctgga
     2761 aaatccagcc tctttcaaat gattgcccaa gttagttctc ttccctatag cggtcaagtc
     2821 ctgatagatg ggagcgaggt cagtcagctt tctatcgtcg aacgtgtcca gacggttggt
     2881 attctcttgc aaaatcctaa tcatcaattt accatggaga gcttgtttga ggagttggtt
     2941 tttaccatgg aaaatatcgg ctatcacctt caggaaattg attctaaaat agcagaggtt
     3001 gtccagcaat gtcgttgcaa ggacatcttg caccgtctca tccatcactt atcaggtggg
     3061 gaaaagcaaa aagcagcgct ggctgtcctc tttgccatga atcctagggt ctatctcttg
     3121 gatgagccct tcgcttccat tgaccgcaag agcagaatcg agatattgga gattctaaaa
     3181 gagttggtct atgatgggaa gacagttatt ttgtgcgacc atgatttatc tgactataaa
     3241 gcctatatcg accatatggt ggagctaaga gacggaaaac taagggaagt gtttcaaatc
     3301 ccttcctatg agatgacaca ggttgcttca aaggaagttg cttctagccc ggaactattc
     3361 catatgaacc gtgtgactgg tgagcttggt aatcgccccc tcttttcaat tgctgatttc
     3421 acattctatc aagggatttc ctgtatcctg ggtgacaatg gtgtcgggaa atcaaccctc
     3481 tttcggtcta ttcttcaatt tcaaaagtat aaggggagca ttacttggaa gggttcggtc
     3541 ctgaaaaaga aaaagagttt gtatcgtgat ctgactggtg ttgttcagga agctgagaag
     3601 cagtttatcc gagtcagtct gcgagaggag cttcaattag atggacctga ttctgaaaga
     3661 aatcagcgga tttttcaagc tttacgatat tttgatttgg agcaggcagt cgataagagt
     3721 ccctatcaat taagtggtgg tcagcaaaaa attcttcagc tcctgaccat cttgaccagt
     3781 aaggcttccg tgatcttgct agatgaacct tttgcaggtt tggatgatag agcctgccat
     3841 tatttttgca agtggattgt ggaggagagg aatcaaggaa gaagttttct gctcattagt
     3901 catcgtttag accctttgat ttctgtggtt gattattgga ttgagatgac tagtcagggt
     3961 cttcggcatg tgaaagaagt gaccattacc aaaccactta catctcagag tagcaatacc
     4021 caaggggagg tgagatagta tggtcaaagt agcaacccag acaccgatta tcagtctctt
     4081 cttgctgatt ttatccttgg aaacatcttt cattccttcg attgctctga atctttcggt
     4141 agtcgcattt tgtattctct ttatgctcta ttaccgtcga tttaaaatgt tagcttggat
     4201 gatcatactt gccattttcc catcttttgc caactactgg gcagttcagt tacacggaga
     4261 tgcttcacag gcagtcatgc ttggaacgag ggcctttgtg acagtttgta tcggccttgt
     4321 ctttgtttcc tctatttcac taaaagagct tctcttgtac ttggctcaaa aggggctatc
     4381 acgctcttgg tcctatgcct tgattgtggt attcaattct tttcctctca ttcagcaaga
     4441 aatcaagtcc ctcaaagaag cttgcctatt acgtggtcaa aaactacatt tttggtcgcc
     4501 cttgatttac agtaaggttc tgatgacagt ctttaggtgg cgccatcttt acctgagagc
     4561 tctatctgct tacggatatg acgaacatgc acagttgaag aatagctatc ggacttttta
     4621 tattcctaaa aaaacaaaat taatctacct gcttttcttt ttattgcttc aaaccagtct
     4681 atttttataa aggagttatt atggaattta cagatattgc gatggaatta tccaagaaag
     4741 cttggcaggc ttcctttcat catcccttta ttttacaatt acaagagggg aatttagaac
     4801 ctgccatttt ccgctattac ctgattcagg atgcctacta tctgaaggct ttctcagaaa
     4861 tctatcatct tttggctgat aagacttcaa accaagagat gaaaagactc ttgaaacaaa
     4921 atgctcaggg tttagtggag ggtgagttat ttattcgcca acaatttttc aaggaattgg
     4981 aaatcaacga ccaggaaatg gagcaacatc caatcgctcc aacctgttat cattacattt
     5041 ctcatattta taggcaattt gcagaaccga acttagccat agcttttgca agcttgcttc
     5101 cttgtccttg gttataccat gatataggca aatcacttaa tcttaaacca tcaccaaatc
     5161 ctctctatca acaatggatt gaaacttata ttacggatga gttagagcaa cagatcagag
     5221 aggagggagc actggtcaat cagctttatc gagaaagtga tgagacagat aagcaaaaaa
     5281 tgctagatgc cttccacatc agtgttcata tggaagccaa gttttgggag atggcttacc
     5341 aacaccagac atggaagagc gatttacagt ctttagaaaa aggagaagaa tagaaacatg
     5401 agaaagcacc aactacaagt tcacaaattg accattttat ctatgatgat tgcccttgat
     5461 gtagtcctta cacctatctt tcggattgag ggaatggcac cgatgtccag tgtagtcaat
     5521 attctagcgg gaatcatgat gggacctgtt tatgccttgg ctatggctac agtgacagcc
     5581 tttatccgta tgacgactca agggattccg cctttagctc tcacaggagc gacttttgga
     5641 gcccttctag caggtctctt ttataagtac ggtcgaaaat tttacttttc tgccttggga
     5701 gaaattgtgg gaacaggtat tattggttct attgtttcct atcctgttat ggtgctcttt
     5761 acaggatcgg ctgctaagct tagttggttt atctacactc ctcgattttt cggagcaacc
     5821 ttgattggta cagcgatttc ctttattgca tttagatttt taatcaagca ggaattcttt
     5881 aaaaaagtgc aaggatattt ctttgctgaa aggatagact gatgcaggca ttgacaaatc
     5941 cttttcctat aggctctagt tccctcattc actgtatgac caatgagatt tcttgtgaga
     6001 tgctggcaaa tgggattttg gctctgggat gcaaacctgt catggcagat gatccccgtg
     6061 aagttcttga ttttactaag caaagtcagg ctctcttcat caatttgggg catttgtcag
     6121 ctgagaagga aaaagcaatc cgcatggcag cttcgtatgc aaaccaatct tctctcccga
     6181 tggtagtaga tgcggttggc gtaacgactt catccattcg taagagctta gttaaagacc
     6241 ttttagacta tagacctacg gtccttaaag gaaacatgtc agaaattcga agtcttgttg
     6301 gattaaagca ccacggcgtt ggggtcgatg cgagtgctaa agatcaagaa acggaggatt
     6361 tgcttcaagt cttgaaagac tggtgtcaga cctatcctgg tatgtctttc ttagttactg
     6421 gccccaagga ccttattgtt tcggagaatc aagttgctgt actggagaat ggctgtactg
     6481 aattagactg gataacaggg acaggagact tggttggagc cttaacagcc gtttttctca
     6541 gccaaggaaa gactggtttt gaagcttctt gcttagcagt ctcttatctc aatatcgctg
     6601 ctgagaaaat agttgttcaa ggaatgggat tggaagaatt tcgttaccaa gtactcaatc
     6661 agctttcgct cctaagaaga gatgaaaatt ggctagatac catcaaagga gaggtttatg
     6721 aatagagaag cacttagact atatctggta accaatcgct atcaagattc cgtggaaagc
     6781 tttcttgcaa aagttgagac ggcctgccgt tcaggggtta ccatagtcca attgcgagaa
     6841 aaaaatctca caaccaatca atattatcaa ctggcaaaac aagtcaagga aataacagat
     6901 gcctatcagg tccccttgat aatcgatgat cggttggatg tttgtcttgc ggttgatgca
     6961 gcaggtctgc atatcggaga tgatgaatta ccagtttcgg tagctcgcaa agtcttgggt
     7021 cctgaaaaaa tcctcggtgt caccgctaaa acggttaaaa gagctctgga agcggagaaa
     7081 tcaggtgcag attacttggg tacaggagcc attttcccaa ccaccaccaa ggaaaatgcg
     7141 cccattactc tgatatcgac cttgaaaaca atttgccaaa cggttgccat tccagtagtt
     7201 gctattggag gcttgacttc ggagaatatt gatcagctta tgggcactgg catagctggt
     7261 gtagctgttg tacgtgattt gatgcaagca gaagatattg aggcaaaaac gcaagccttt
     7321 ttaacaaagt tgcatgacat tctttcctaa ttaatactca atgaaaatca aagagtaaac
     7381 tagaaagcta gtcgcaggtt gctcaaagta ctgccttgag gttttagata agactgacga
     7441 agtcagctca aaacagtgtt ttgaggttgc agatggaagc tgacgtaatt tgaagagatt
     7501 ttcgaagagt ataaaaactc tctaattccc ttaaagtggg aactagagag tttttttctt
     7561 aatctttaaa agttgtatgg ttgactggac cagaaccatg accgagttga ggggcatctt
     7621 gaatagcttt tgtgataaag gccttggcct tatcaactgc ctggtaaaga ctcttgccct
     7681 tggctagttc agcagtaatc actgcagcaa aggtacatcc agtaccatgg gtgtgacagg
     7741 tttgaattcg tgggctttcc cagacaaatt gttcattctt ggtaaagagg aaatctttag
     7801 caccaccttt gagatgtccg cctttgataa ccacagactg aggaccaaat tcttttaaaa
     7861 tcaggcgacc agcacgctgc atgccttcgg ggtcatggat tgaaaaacca acaatctctt
     7921 ctgcttcagg aagatttggc gtaataatag ttgctagagg tagtaagttt gttttgagat
     7981 agtctctagc acttgagtca atcaaggcat ctccacttgt agcaaccata acaggatcaa
     8041 ggacataggg acaatccagt tttttaagat agggttggat gatttccatg atttcagtag
     8101 tagccaacat tccagttttt acagcctgag gtggaatatc agaaaagaca ctctccaatt
     8161 gggctttcaa catttgagga gaaacgtgct cgattagctg aacacctctg gtattttgag
     8221 cgacaagact ggttacaaca gccattccat agacatctct cgcttggaat gactttaaat
     8281 ctgccataat gccagcacca ccactagggt cagtccctgc aatggtcaaa gcaacgggta
     8341 aataagtcat ctaaaacctc ccaaccaact gaagtttgta ttcttaagaa caagctagtc
     8401 tgttttagaa aggcaataga agactgctag tccccattat catttccact tccatcagct
     8461 agcattacct agattgagtc agagggatct aacagtcgtt aatctcagct ttacgcaccc
     8521 ctagtggttg ttttcttttt tctttagtat agcatatttt tattgtttat atagtaatat
     8581 ttgactgcaa atcgtttttc aaatgattaa aattctattt ttcgaagaaa atattatttc
     8641 tataattgac aaatgtagat tttaagagta tactgatgag tgtaattgac aaatgtagat
     8701 tttggaggta tggtaatgca gatttcagat gcagaatggc aggtcatgaa gattatttgg
     8761 atgcaggggg agcagaccag tacagatttg attagggttt tggcagagcg gttcgactgg
     8821 tccaagtcga ccattcaaac tcttttggct cgtttggttg agaaagagtg tctgactcgg
     8881 aaaaaagaag gcaagtcctt tgtttattca gcccttttaa ctctagacca aagtcgggat
     8941 ttacttgtcc aagatatcaa agacaaggtt tgttcccgta ggattaggaa tttgttggct
     9001 gacttgattg tagaatgtga atttactcag actgacttgg aagacttgga agctgtgatt
     9061 tcagagaaga aatcaagtgc tgtaacagaa gtaagatgta attgtatgta aaggagacgt
     9121 catgttaaat agtattgtaa ccattatttg tattgccctt atcgcgttta tcttgttttg
     9181 gtttttcaaa aagcctgaaa aatctggaca aaaagcccag caaaaaaacg gataccaaga
     9241 gattcgagtg gaagtcatgg gaggctatac tcctgagttg attgtcctca agaaatcagt
     9301 gccagcacgc attgtctttg accgcaagga tccttcacca tgtctggatc aaattgtttt
     9361 tccagatttt ggtgtacatg cgaacctgcc aatgggggaa gagtatgtag tggaaatcac
     9421 gcctgagcag gttggagagt ttggctttgc ttgtggtatg aacatgatgc acggcaagat
     9481 gattgtagag taggtggaga ctatgacaga aattgtgaaa gcaagcttag aaaatggcat
     9541 tcaaaaaatc cgtatccgag ctgaaaaagg ctatcatcca gcccatatcc agcttcaaaa
     9601 gggaattcca gctgagatta cctttcatcg tgctactcct tcaaactgtt ataaggaaat
     9661 tctgtttgaa gaagaaggta tcttggaacc aatcggcgta gatgaggaga aagtcattcg
     9721 ttttacacct caagaattag ggcgacatga attttcttgt ggcatgaaga tgcaaaaggg
     9781 aagctatata gtcgttgaga agactcgaaa atctctatct ctcctgcaac gtttttggat
     9841 tactagtatc tttactgtgc ctcttgtgat tctcatgatt gggatgttga caggtagcat
     9901 tagtcatcaa gtcatgcatt ggggaacctt tctagcaaca acgcctatta tgttagttgc
     9961 aggtaagcca tatatccaga gtgcttgggc cagttttaaa aagcacaatg ccaacatgga
    10021 taccttggtt gcgctgggaa ctctagtggc ttatttctat agcctagttg ctctctttgc
    10081 tggtctccct gtttacttcg aaagtgctgg atttatcctc tttttcgttc ttttgggagc
    10141 agtttttgag gaaaaaatga gaaaaaatac gtcccaagct gtggagaaat tactggactt
    10201 gcaagctaaa accgcagaag tcttgagtga tgatagttat gtccaagttc ctttggaaca
    10261 agtcaaggta ggcgacctga ttcgagtgcg tcccggtgaa aagattgctg ttgatggtgt
    10321 cgtagtagaa ggtgtctcta gtattgacga atccatggtg acaggtgaga gtctgcctgt
    10381 ggacaagaca gttggagata ctgtcattgg ctcaaccatc aatcatagtg gaacgcttgt
    10441 ctttagagca gaaaaagttg gctcagagac tgttttggct cagattgtgg attttgtgaa
    10501 gaaagctcag acaagtcgtg cgccgattca ggacttgacg gataagattt cagggatttt
    10561 tgtcccagta gttgtcattt taggaatcat gaccttttgg gtttggttcg tcttgctcag
    10621 ggatagtgtg gtcgtgcttg gagctagctt tgtgtcctct cttctctacg gagtggcggt
    10681 tttgattatc gcctgtcctt gtgccttggg acttgcaaca ccgacagccc ttatggtggg
    10741 gacaggacgt agtgccaaga tgggggttct cctcaaaaat ggaactgtct tacaggaaat
    10801 ccagaaagtt caaactcttg tctttgataa gaccgggact ttgacggaag ggaaacctgt
    10861 ggtaacagat atcatcggcg acgaagtaga agtgtttgga ttggcagcct ccttggaaga
    10921 tgcttctcaa cacccactgg ctgaggctat cgttaagcga gcgagtgaag ctggacttga
    10981 gtttcaaact gttgaaaatt ttcagtcctt gcacgggaaa ggtgtttcag ggcgaatcaa
    11041 tggaaaacaa gttttacttg gaaatgctaa aatgctggat ggtatggata tttctaatac
    11101 ttatcaagat aaactagaag aactagaaaa agaagctaag acagttgtgt tcttagctgt
    11161 tgacaatgaa atcaaaggct tgcttgcttt gcaagatatt cctaaggaaa atgctaagct
    11221 agccatcagt cagctaaaaa aacgtggtct ccgaacagtc atgctgacag gagacaatgc
    11281 tggtgtggcg cgtgctattg cagatcaaat cggaattgaa gaggtcattg caggcgtctt
    11341 gccagaagaa aaagcccatg aaatccataa actgcaacag tcagggaaag tagcctttgt
    11401 tggggacggt atcaatgacg ctcctgccct tagtgtagca gatgtgggga ttgctatggg
    11461 agctggaaca gatatcgcca tcgagtcagc agatttggtg ttgacaacca ataatctttt
    11521 aggagtggtt cgtgcctttg atatgagtaa gaaaaccttt catcgaattc tactcaatct
    11581 tttctgggct tttatctaca atgttgtcgg aattccgatt gcagcaggag tcttttcagg
    11641 tgttggactg gctctcaacc cagaattggc aggtctagcc atggccttta gttctgtatc
    11701 tgttctgacc agttcccttc tgttaaattt tagtaaaata gactaaaagt aatgactaga
    11761 tttctttgtt ataaaacaga aatgacagaa ctttcaaaac tgtattagag tagagaatag
    11821 agagttttga gcagattttt agaaaagtca gcataaatat gatacagtgg aatagtaaaa
    11881 atttggagaa cgtttccaat tctatgtaat cgtattctcc aaatctaaaa aaattgaagg
    11941 agagttatca ttatgactca agggaaaatt actgcatctg cagcaatgct taacgtattg
    12001 aaaacatggg gcgtagatac aatctacggt atcccatcag gaacactcag ctcattgatg
    12061 gacgctttgg ctgaagacaa agatatccgc ttcttacaag ttcgccacga agagacaggt
    12121 gctcttgcag cggttatgca agctaaattc ggcggctcaa tcggggttgc agttggttca
    12181 ggtggtccag gtgcgactca cttgattaac ggtgtttacg atgcagctat ggataacact
    12241 ccattcctag cgatccttgg atcacgtcca gttaacgaat tgaacatgga tgctttccaa
    12301 gagcttaacc aaaacccaat gtacaacggt atcgctgttt acaacaaacg tgtagcttac
    12361 gctgagcaat tgccaaaagt aattgacgaa gcctgccgtg ctgcaatttc taaaaaaggt
    12421 ccagctgttg ttgaaattcc agtaaacttc ggtttccaag aaatcgacga aaactcatac
    12481 tacggttcag gttcatacga acgctcattc atcgctcctg ctttgaacga agttgaaatc
    12541 gacaaagctg ttgaaatctt gaacaatgct gaacgcccag ttatctatgc tggatttggt
    12601 ggtgttaaag ctggtgaagt gattactgaa ttgtcacgta aaatcaaagc accaatcatc
    12661 acaactggta aaaactttga agctttcgaa tggaactatg aaggtttgac aggttctgct
    12721 taccgtgttg gttggaaacc agccaacgaa gtggtctttg aagcagacac agttcttttc
    12781 cttggttcaa acttcccatt tgctgaagtt tacgaagcat tcaagaacac tgaaaaattc
    12841 atccaagtcg atatcgaccc ttacaaactt ggtaaacgtc atgcccttga cgcttcaatc
    12901 cttggtgatg ctggtcaagc agctaaagct atccttgaca aagtaaaccc agttgaatca
    12961 actccatggt ggcgtgcaaa cgttaagaac aaccaaaact ggcgtgatta catgaacaaa
    13021 ctcgaaggta aaactgaggg tgaattgcaa ttgtatcaag tttacaatgc aatcaacaaa
    13081 catgctgatc aagacgctat ctactcaatc gacgtaggta acactactca aacatctact
    13141 cgtcatcttc acatgacacc taagaatatg tggcgtacat ctccgctctt tgcgacaatg
    13201 ggtattgccc ttcctggtgg tatcgctgct aagaaagaca atccagatcg ccaagtatgg
    13261 aacatcatgg gtgatggagc attcaacatg tgctacccag acgttatcac aaacgttcaa
    13321 tacgaccttc cagttatcaa ccttgtcttc tcaaatgctg agtacggctt catcaagaac
    13381 aaatacgaag atacaaacaa acacttgttt ggtgtagact tcacaaacgc tgactacgct
    13441 aaaattgcgg aagctcaagg agctgttgga ttcacagttg accgtatcga agacatcgat
    13501 gcagttgttg cagaagctgt taaattgaac aaagaaggta aaactgttgt catcgatgct
    13561 cgcatcactc aacaccgtcc acttccagta gaagtacttg aattggatcc aaaacttcac
    13621 tcagaagaag ctatcaaagc cttcaaggaa aaatacgaag cagaagaact cgtaccattc
    13681 cgtctcttct tggaagaaga aggattgcaa tcacgcgcaa ttaaataatt cctctcgccg
    13741 aaaatcaaat atgaaacttg tagactttat tctttgattt tcatagagca gaactttaaa
    13801 agatcggagc aattcggtct ttttatggag gagagtaaat gaatttaaat caattagata
    13861 ttatcgtttc aaatgttccc caagtctgtg ctgacttgga gcatattttg gataaaaagg
    13921 cagattatgc tgatgatggt tttgctcagt ttacgattgg tagtcactgc cttatgttgt
    13981 cacaaaatca tttggttcct ttggaaaact ttcagtcagg aatcattatt catatcgagg
    14041 ttgaggatgt agaccagaac tacaaacggt tgaatgagct tggtataaag gttttacacg
    14101 gaccaactgt aaccgattgg ggaacagagt ccttattagt tcaaggacct gctggtctag
    14161 tgcttgattt ttatcgtatg aaataggtcg cgtagtcatt tgacgttaac cttagctatt
    14221 tttaaaatag aaattgagaa tatgaactat tgaaagatcg gagcaatccg gtctttttat
    14281 ataaagaaat tgcatat
//
LOCUS       DAVBCG010000041        14005 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBCG010000041 DAVBCG010000000
VERSION     DAVBCG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.041"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(114..1964)
                     /locus_tag="VQ495_001030"
     CDS             complement(114..1964)
                     /locus_tag="VQ495_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2531002.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2032..3171)
                     /locus_tag="VQ495_001031"
     CDS             complement(2032..3171)
                     /locus_tag="VQ495_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2531003.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            3229..4395
                     /locus_tag="VQ495_001032"
     CDS             3229..4395
                     /locus_tag="VQ495_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2531004.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            4534..4860
                     /locus_tag="VQ495_001033"
     CDS             4534..4860
                     /locus_tag="VQ495_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2531005.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            4847..5440
                     /locus_tag="VQ495_001034"
     CDS             4847..5440
                     /locus_tag="VQ495_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2531006.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            5433..6344
                     /locus_tag="VQ495_001035"
     CDS             5433..6344
                     /locus_tag="VQ495_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2531007.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            6407..7471
                     /locus_tag="VQ495_001036"
     CDS             6407..7471
                     /locus_tag="VQ495_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531008.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7543..8403)
                     /locus_tag="VQ495_001037"
     CDS             complement(7543..8403)
                     /locus_tag="VQ495_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2531009.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHIYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(8525..9511)
                     /locus_tag="VQ495_001038"
     CDS             complement(8525..9511)
                     /locus_tag="VQ495_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2531010.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(9783..11258)
                     /locus_tag="VQ495_001039"
     CDS             complement(9783..11258)
                     /locus_tag="VQ495_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2531011.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(11362..12285)
                     /locus_tag="VQ495_001040"
     CDS             complement(11362..12285)
                     /locus_tag="VQ495_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2531012.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(12299..13228)
                     /locus_tag="VQ495_001041"
     CDS             complement(12299..13228)
                     /locus_tag="VQ495_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2531013.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(13547..13897)
                     /locus_tag="VQ495_001042"
     CDS             complement(13547..13897)
                     /locus_tag="VQ495_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531014.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
BASE COUNT         4187 a         3025 c         2582 g         4211 t
ORIGIN      
        1 acggcaaggc gacgttgacg tgatttgaag agattttcga agagtataag tctctacata
       61 ataaaacgca tattaccaag gttatttaac ttgataatat gcgtttttct tttttattca
      121 atgtgggttg tttgattagc aaaggcgata atagcttgct tcaactcatc tccactgaga
      181 gtgcggaact ctccaatctt ttgattgatg gattctaaag gcatgacatt aaccttacca
      241 acgaaaatct tatccataac ttgattatca acgagttcgg ttgataccaa gagttcttcg
      301 tagagttttt cacctgggcg gattccaact tcaacgattg gaatttcact ttcagtgtgg
      361 ccacttagaa gcaccatctt cttggccaag tcataaatct tgactggttt gcccatatca
      421 aggataaaga cttccccatc tttggcataa gcaccagcat ggataaccag acggctagct
      481 tctggaatgg tcataaagta acgggtcatg cggaagtctg tcaccgttac aggcccacct
      541 tcagcaatct gacgttcaaa gactggaatg acactaccac ggctaccaag aacattccca
      601 aaacgaactg cacagtaggt tgattggcta cgttggttaa agccagtgac aatcaactcc
      661 gccacgcgct tggttgctcc cataacattt ggtggattga ctgccttatc tgtcgaaatc
      721 ataaccatct taggcacctt ggcttcatca acggctctag caacattgta agttccacgg
      781 atattgtttt tgaaggcttc ttttggattg cgctccatca taggaacatg cttgtgggct
      841 gccgcatgat aaacaatagc aggtttgtac tgctcaaaga cttgcaacaa acgatcatag
      901 tcttgaatgt ccgcaatcac aggtacataa tcaatccctt ggaacttacg aatcaattca
      961 tgataaacaa ggtagattga gttttcccca tgaccgagca agacaatgcg ttcaggattg
     1021 aagcgactaa cttgacgaca gatttcagaa ccgattgaac ctccagctcc tgtgactaag
     1081 atggtcttac ctgtcagttc tgcacccaga cgcgattcgt caagacggat ttcctgacga
     1141 cccaaaaggt ccgtaatatc aattttttgg aagccagtac ctgcttggtg aaggccctga
     1201 acaacagttt caaccttagg catcttgtaa catttgacac ccagcttatt acacatctgc
     1261 aagatacgct catattctga cggatccagc gacggaatcg caacgatgac acgctcgatt
     1321 tgatggcgtt tggctaattc aggcagattg tcataagagc ccaaaacagg aataccacca
     1381 agtttttgac cctttttctt agaatcctta tccaaaatac cgaccagttc taattcactg
     1441 gttggatgtt ggtaactatc cataaagaga gccccaccat caccggcacc aatcaagaag
     1501 gtccgacggt gttctccatc accactacct tttttgcgtc tggagtagat taactgccaa
     1561 gtaatccgtg gcaataaaat caagaaggta ctcaacaaga taaagagaat gataaaacgg
     1621 atggagaaga gtggcaagaa ggcataacag atactatatg acaagacact gctagcagtc
     1681 acaccaaaaa agattttcat gaaatccgta atcttgctgt aacgactaat actcgcgctc
     1741 aagccccaaa aatcaaccat caattgatag aacaggaagg ccaaactcgt atagataatg
     1801 tagtcaacag gtgctggatt aatcagccca taaaataaaa tataagatac aatgatggaa
     1861 accaccatac tgaaaatatc aaatattccc cagaaaacct gcttttgttg tttatttaaa
     1921 atttccacca gatcaatcac ataatctgtg agttttttat tcatagaaat ttactcctcc
     1981 ttaaaatagt tctactatat tgttattata gcacatttta atttatttgg cttatttttc
     2041 caaataaatt actgttccct tgtactcctt cttccattta aatcctctaa ttaaacctac
     2101 aatcgtccca aggccataag caaagtgaat ggaaaataaa agaaagggca tcacaattag
     2161 aaatccattt ttatgtttta ataaagtcag caaagtgagc aatgacagta atgataaaag
     2221 tagaaaatag gcacctaata gtaaagttat gaatacgaat gtgatcggta acaatgctag
     2281 actaaacaca agactcaaaa caaataaaca aggaacataa tgaaataatg ataaacactt
     2341 aaactgaaca tgacttgtca agccaatcca caaaccattt gaatactttt gatgcagcat
     2401 tttcttgaat gttggtcgaa tatactgata agatagaata cttgggctat agcggatttt
     2461 ataaccatgt tctcgaattc tataatgaat atcattatct tcagttcggc caagttgctc
     2521 atttactaaa ccaaccttct ggaaaacctc tcgtttatac atcccatgaa aaatagaaga
     2581 aacatatcta tcctcggaac tatttcgata attggcaatg ctactgccaa acatattttc
     2641 ctcaacaaga tgcaaggtct ctgcccattt tccttttcct ttgacaatcg tcggtctagg
     2701 ccctccacag acaaattcac cttgttgaat aacagccaca ttgttcatta caaaactctc
     2761 agtaactttt gaatgagcat caatttttaa aataaggccc cctacagaat gcttaactcc
     2821 caggttaaaa ccactagctt gatttttctt aggattgtta tacaatctaa ttgagttaaa
     2881 ctctgcatct tcttttataa attgctgaat gatagctgtg gtcccatctg tggacatagc
     2941 atttataaat agaatttcaa tattctcttt aggataggtt tgatttttta agtcttcaat
     3001 tagaccaggc agatattttt cttcattgta tgcactgatt acaatggata cgagaagact
     3061 ttcaacattt tgagtcacta ctactctcct gaattttccc ttccaccatt ctatcataat
     3121 tttcaagact tttccatttt tatcttgaaa gccagaaacc ttacttgaca ttatagtgaa
     3181 aaagccttaa aataagctgt tattaaaatc agctagaaga gatattatat gaaaaagcaa
     3241 tcactctttt ttgttccagg tattatcctg attggtgttt ccttgcgaac tccttttact
     3301 gttttaccca ttattttggg aaatatttcg caaggtctgg aggtagaagt tagttcgctt
     3361 ggtgtcttga ccagcctgcc tctccttatg tttaccctct tttcaccatt ttctacccga
     3421 ctggctcaga aaatcggctt ggagcatctc ttcacctaca gcctcttctt cttgaccatc
     3481 ggctcactta ttcgactaat caatctgccc ctgctctatc taggaacctt gatggttggg
     3541 gcaagtgtcg cagtcatcaa tgtcctgctt cctagtctta tccaagccaa tcaaccaaag
     3601 aaaattggtt ttctgaccac cttatatgta acgtctatgg ggattgcaac ggctctggct
     3661 tcctatctag ctgtgcccat tacacaagcc agttcttgga aaggacttat cctcctcctc
     3721 acgttactct gtctagcaac ttttttggtc tggctcccaa atcaccgcta taatcataga
     3781 ctagctccac aaaccaaaca aaaaagtcaa ataaaggtca tgcgtaataa acaggtttgg
     3841 gcaattatta tcttttcagg ttttcaatcc ttgatctttt acaccgccat gacctggtta
     3901 cctaccatgt ctatccatgc aggtctatcc agtcacgaag ctggcttgct gacttctatc
     3961 ttatctctga ttagcattcc tttttcaatg accatcccaa gcctgacaac cagtttatct
     4021 actcgcaacc gtcagctcat gctcactctg gtttcactag ctggtgtggt cggcatttcc
     4081 atgctctttt tcccaatcaa taatttcatt tactggcttg ccatccatct cctcatcgga
     4141 accgcaacca gcgccctctt cccttatctc atggtcaact tttcactcaa gacaagcgcc
     4201 cctgaaaaga cagcccaatt gtccggccta tctcaaacag gaggctatat cctagcagcc
     4261 tttgggccaa ccctctttgg ttacagtttt gacctattcc actcttgggt accagctgta
     4321 gctgccctct tgctcatcga tatcctgatg actgtggccc tctttacagt ggacagagcg
     4381 gataagatcc tttaaacttc tagttttagc tagaagtttt ttatatataa aaattttaca
     4441 catttctctt gacagggctt tcttttagat gtacaatgta tgtgtagaaa aattatatat
     4501 aaaaatctta cacattagaa aaggaggttt cccatgtact ttccaacatc ctctgccttg
     4561 attgaatttc tcatcttggc tgtactggag cagggtgatt cttatggtta tgagattagc
     4621 caaaccatta agctgatcgc taatatcaaa gaatccacac tctatcccat tctcaaaaaa
     4681 ttggaaggca atagctttct gacaacctat tctagagagt tccaaggtcg catgcgcaaa
     4741 tactactcct tgacaaacgg tggtatagag cagctcttga ccctaaaaga tgaatggaca
     4801 ctctatacag acaccatcaa tggcatcata gaagggagta tccgccatga caagaactga
     4861 atacctgact cagctagaac tctatctcaa gaaactacct gaagctgacc gtatcgaagc
     4921 catggactat ttcagagagc tctttgacga tgctggagtc gaaggagaag aagaactcat
     4981 cgctagtttg ggaactccca aagaagtggc ccacgaagtt ctatccaatc ttctcgataa
     5041 aaaaatcaat gaagcacccg ctcaaaaaaa taaccgacaa attttacata tcgccttgtt
     5101 agccctcctt gcagcaccta tcggcattcc tctgggaatc gccatcctcg tgaccctgtt
     5161 cgcaatcctt gtagccgctt tgactgtcat tctggctttc tttgcagttt ccatactggg
     5221 tatcatcggc ggattcctat ttttagttga aagtttcact gtcctcgccc aagccaaatc
     5281 agcctttatc ttgatttttg gttctggttt actggctatc ggtgcttctt cgctagtttt
     5341 acttggcatt tcctatgtag ctcgcttctt cggtctactc attgttcgtc tggtacaatt
     5401 tgttcttaaa aaaggaaaga gaggtaatca gcatgcgtaa atggacaaaa ggatttctca
     5461 tctttggtgt ggtgactacc gttatcggct ttatcctgct ttttgtaggt atccaatctg
     5521 acgggattaa gagcctactt tccatgtcca aagaacctgt ctatgatagc cgtacggaaa
     5581 agctaacctt tggcaaggaa gtcgaaaacc tagaaattac tctccaccaa cacacgctca
     5641 ccatcacaga ctctttcgat gatcaaatcc acatttctta ccatccatct ctttctgctc
     5701 accatgatct tatcaccaat cagaacgata gaactctgag tctcactgat aagaaactgt
     5761 ctgaaactcc gtttctctct tctggaattg gtgggattct tcatatcgca agtagctact
     5821 ctagtcgttt tgaagaagtt attctccgac taccaaaagg gagaactcta aaagggatca
     5881 acatctcagc caatcgcgga caaaccacca tcataaatgc tagccttgaa aatgcgaccc
     5941 tcaatacaaa cagctatatc ctccgaattg aaggaagtcg tatcaaaaac agtaaactca
     6001 caacgcccaa tatcgttaat atctttgata cagttcttac agatagtcag ctagagtcaa
     6061 cagagaatca cttccacgct gaaaatatcc aagtccatgg taaggttgaa ctgactgcca
     6121 aagattatct cagaatcatc ctagaccaga aagaaagcca acgaattaac tgggacatct
     6181 caagcaacta tggttctatc ttccaattca caagagaaaa gcctgaatca agaggtacgg
     6241 aattaagcaa cccttacaaa actgaaaaaa ccgatgtcaa ggatcaactc attgcgagat
     6301 ctgatgataa tattgatcta atatccacac caagcagacg ttgacaaaaa cgcttacatc
     6361 tgctactatg gaagcatatt tatctaacta aaaaggaggt tctaccatga aacaagaatg
     6421 gtttgaaagt aatgattttg taaaaacaac aagcaagaac aagcctgaag agcaagctca
     6481 agaggttgca gacaaggctg aagaaacgat agccgatctc gatacaccaa ttggaaaaaa
     6541 tactcaagta gaggaggaag tctctcaagc tgaagtcgaa ctggaaagcc agcaagaaga
     6601 gaaaattgaa actcctgaag gcggtgaagc gagaacagaa atagaagaaa agaaggcatc
     6661 taattctact gaagaagagc cagacctttc taaagaaaca gaaaaagtca ctatagctga
     6721 agagagccaa gaagctcttc ctcagcaaaa agcaaccacg aaagagccac ttcttatcag
     6781 taaatcttta gaaagtcctt atatccccga ccaagctcca aaatctaggg ataaatggaa
     6841 agagcaagtg cttgattttt ggtcttggct agtggaagcg atcaaatctc ctacaagtaa
     6901 gttggaaaca agtatcacac acagttacac agcctttctc ttgctcattc tgttttctgc
     6961 atcttccttt ttctttagta tctatcacat caaacatgct tactatggac atatagcaag
     7021 cattaacagt cgcttccctg agcagctagc tcctttaact cttttttcta tcatctctat
     7081 cctagtagcg acaacactct tcttcttttc attcctcttg ggtagtttcg ttgtgagacg
     7141 atttatccac caggaaaagg actggacgct agacaaggtt ctccaacaat atagtcaact
     7201 cttggcaatt ccaatcttcc tcactgctat tgctagtttc tttgctttct ttgatagcct
     7261 acgatttacg gccctcttgt gtgtgattag cattggaatc attctgcttg ccagtctcca
     7321 tatcattaca agaccaagtc aagcaagtga aaccgactcc ttctatcaat tattcttgtc
     7381 tgtccttgtg aacggagtta ttatcctcct cttctttgta gctgaagtgg cactgattgg
     7441 agattatctt cgtatcttgg cctttcttta aaattcaatc ctgtcatcct tggcaggatt
     7501 ttttctatgt caaaaaagca agtcgattga cctgcttctg ctttactttt cttgtgctaa
     7561 tgttttaaaa tctttgtcta atatgggttg cacttctatt tgattggcgt ctagttggtg
     7621 gtaaaaagat gggggtaata cctctaagaa ttttgcatag tccaagcgag caatggcttc
     7681 atagtcttct ggtttagagc catctcctgt gatttgaacc aagtcaatct cccactgcac
     7741 ttccttatcc accaattgct caatataggc tgcaggaaca aacggttctc tcggtaaaac
     7801 tgtcttgact ccttgagcta gatggtaaac gccatgcact tttccttctc catcctgata
     7861 gaaggaaact ctcgcaaagt aggcatctgt ctcttgaacc gcacgcacac gcgcttcaaa
     7921 ctgagccaaa ccatctgcct ctaaaaagct tttcaagttc tcacgactaa agaaaatagg
     7981 attaaaaatt ccttggcaaa tcgtaaaacg agctacaggg tctgctagat aggcttgaat
     8041 acttgcttgg aaataagctt caaaatcaaa accatctagc ccttcaatat cagtccctgt
     8101 ataagcaagc aaagcatcta aaaatgactt gataatctgc cagtctgtct ctgttagtag
     8161 gtcaggaaga tcgacacggt aagccttttg accatcagca taactgacct taaaaagaaa
     8221 ttgtgattgc cccactatcg cacactcaat atactcgaga cgattaagag gctgacggag
     8281 atagactaca tcatagatat gtgactccaa accgtctagc aatgccaaga tcgacttggc
     8341 attcaaaatc tcttgttctc ctaaaatgct ctgtttattt ggaataaaaa atgttttcgc
     8401 cataactgga ctcctttgaa atcgcttaca tacagtatac cttatttttc tgaaagatac
     8461 agaaacaaac tttagatttt tgagtacaaa gcatagaaaa acagcccctg aggactgttt
     8521 gatcttatac agtagctgct tgatccaagc tttcaccgat agcggctagg cgctcgatca
     8581 cttcagcttg tgtcaattca ttttttgaaa catagcggtt acgtgggtga acacggcact
     8641 cgtgtgagca tccacgaagg tacttgtctt cattttcttc tgatgtcaag atacgacggt
     8701 tacagaaggg atttccacag ttgacataac gttcacatgg tgttccatca aaccagtctt
     8761 tccctacgat ggttgggttg acatggttga catcaacggc gatacgctcg tcaaatacgt
     8821 acatttttcc atcccaaagc tcaccttgaa cttctgggtc tttaccgtaa gttgcgattc
     8881 ctccgtgcaa ttggccgaca tctttgtagc cttcacggac catccagcct gagaatttct
     8941 cacagcgaac gccacctgta cagtaaacca cgacacgctt gtccatgaat ttttccttgt
     9001 tatcacggac ccattgtggt aactcacgga agttgcgaat atctgggcga atagctccac
     9061 ggaaatgtcc taggtcgtac tcataatcgt tacgtgtgtc aaggacaacg gtatctttat
     9121 caagaagcgc ttctttgaac tcttttggag acaagtaagc acctgttgtt tcaagtgggt
     9181 tgatgtcatt gtcaaagtcg ttgtcttcca aaccaaggtg gacaatttct ttcttgtagc
     9241 gaacaaacat cttcttgaag gcttgttcat tttcttcgtc aatcttgaac cagagttctt
     9301 ccattcctgg aaggctgtga acgtagtcca tgtatttttg agttgtttca tagtcacctg
     9361 aaactgttcc gttaattccc tcgtcagcga ctaggatacg gcctttaaga ccgattgatt
     9421 tacagaaagc caagtggtct gcagcaaatt gctctgcatt ttcaattgga gtataaaggt
     9481 agtaaagtaa gacacgaata tcttttgcca taagatttgt tctcttttct attcttaaat
     9541 tttctaaatt ttttatttat ccatactagt atacctgctt gagaaaacga atgcaattta
     9601 tttgactgga aattgtagag gattctcgaa atttcctgaa tggttaaaat aaggacaaga
     9661 gaaaacatgg atatctatat ccttgtgcca aaaaaaccac tgccctcccc agaccaacct
     9721 gaggaaagca gtgattctta ttttaggagt taggaatgaa tacacgaaat caatttagct
     9781 gattattttt tgtttttcaa gaattcatcg tattgttttt gcatttcgtt caatactttt
     9841 tcgtaggcac cttcagattt caatttttcc atcaattctg gaatcgcttt atctgggtct
     9901 acagtaccag tgttgatagc tgtatcaaat tgttgcattg tgttagcaat agctgagatt
     9961 tcagatttca cattgtcagt attgaagata aatccaagcg ctggagattc tttagcttct
    10021 gccaattctt tcttagaatt ttcgatttgt tggtctgtaa cgttttcgtt gatgtaaagg
    10081 atccagttgt taccagtgtt ccatccaccc atgtgagtgt ttcctttgta gccatcaaga
    10141 acgcgaacac ggttttcttt accttcaatt ttttcccagt tcttgccttc tggaccgtaa
    10201 acaagaccgt tcaaaagttc tgggttcgta ttcaagaggt tcaagatttc cattgatttt
    10261 tctttgttct tagagttgtt tgagatgaca aagttagcaa cttgtgttgt ttggtttttc
    10321 ttgatgaagt tagtgattgg tttgatttgg atatctttgt tggcaacacg tgaaagcaag
    10381 ctgttaccgt agtcagctgg tcctactgtt tcttcacgaa cgaaccaagt atcttgttga
    10441 aggtcaaatg aagtatcgct tgttgcgacg tcttttggaa tgtagccagc ttcatagaat
    10501 ttgtgaagag tcttcaagtg ttctttgaaa cgaggcactt cgtaacggtt tacaacttta
    10561 gtagtatcgc cttcaaggtc aataacgaat ggaagcccgt ttgctactgg gtagtcaaaa
    10621 ttatcagatg ggatgaaaac tttaccaata gcaaatggta ctacgtctgg agctttttct
    10681 ttgatttgtt tcaagactgg ctcaagagtt tcgtaagaag taacacctga aatatcgata
    10741 ccatatttgg caaggagagt tccgttgaag gcaaagtttt gagatgatgc tacgttggct
    10801 gcaactggta cagcataaat cttaccattt acagtattac ccttgatata agctggatca
    10861 agtgccttgt aaaggtcttt accttctttt ttgtacaatt ctgtcaagtc agcataagca
    10921 cctttttgag catttacaat atagttatct gcaaaggcga tatcatagtt ttcaccagat
    10981 gatgtgataa ctgacatttt cttaccatag tcaccccagc caaggtattg gatatccaat
    11041 ttggcaccaa ctttttcttc gatgattttg ttggcatttg ctaacaattc atccaagtta
    11101 tctggtttgt caccgatttg gtacattttg ataacaggtt tgtcacctga atcagcagct
    11161 tttttgctgt tacctgtcaa atttccacaa gcagcaagac ctgcagccag agcgactaca
    11221 ctagcagatg caaaagcata ttttttccag tttttcatga taaaaactcc tttttttatt
    11281 tttaaactta taaacaatgt aatgatctta acttcacaca agggaagttg ggaactgaga
    11341 aatgttcttt ttcaataagc actattcttt cacaccaccg atagtcaaac cttttacaaa
    11401 gtagcgttgg aagaatggat acaaaatcgc gattggaagg gttgctacca caaccatggc
    11461 catacgacct gtttctttcg gtagagcaac tcccagttga ccagtcaagc cgaccgcttt
    11521 ggcaatgtag tccatatttt gttggatttg catgagcaaa tattgcaatg gatacaagtt
    11581 atcactcttg atgtaaagaa gggcgttgaa ccagtcattc cagaaaccaa gagctgtcaa
    11641 aagcgtgatg gttgcgatac ctggtagtga caatggcaaa cagatttgga agaaaatccg
    11701 agcctcactg gcaccatcga tacgagccga ttcgagaatg gcttctggaa tggtcttctt
    11761 gaaaaaagaa cgcatcaaga tgatattgaa tggtgagaga agcattggaa caatcaaggc
    11821 ccaaacagtg tcaccaagct gaagtacacg ggtcaccatg atataacctg gtaccaaacc
    11881 agcgttgaac aacatactga gaaggacgaa gatggtaaaa aatctgcgat acttaaaggt
    11941 tgtccgtgaa atagcgtagg cataggttgt tgtgataaag acatttgtca atgtcccaac
    12001 tacggttaca aagacagaga tgaagagggc ttgtaggatt ttatccttaa actgtgccaa
    12061 aaactcaaaa ccgtctaagc caaattggga tgggaagaag ctatagccgt attggaggag
    12121 gcttttctcg tctgtcactg aaataatgat aacgaataca aaaggtagga tacaagagag
    12181 ggcaatcaaa cccgaaatga tactgaagaa gatatctgct ttcttactga aggagtgaat
    12241 gccgacatta tcaatttttt cttttttaat tttctttttt gccatattct cctcctttct
    12301 agaacaaagc tgagtttgga tcgactcgtc ttgcaagcaa gtttgatagg ataaccagaa
    12361 tcaaaccaac aacggattgg taaagaccgg ctgctgcagc cataccgata tctgctgtct
    12421 gagtcaaacc attaaagaca tatacgtcca aaacgttggt tacattgtaa agctgaccag
    12481 cattgtgtgg gatttgatag aagagaccga agtctgcgcg gaagatattt ccgactgcaa
    12541 ggatggtcaa tacagttaca agcggagtca actgaggaat ggttacgttg cgaatacgtt
    12601 gccacttgct agctccgtcc actgtcgctg cttcgtagta ggttggatca attcccatga
    12661 tcgtcgcata gtacatgaca ctgctatatc caaagccttt ccaaatacct aggaaaagta
    12721 ggagataggg ccagatgccc aggtcagcgt agaaattgac ttctttgaga ccaagacttt
    12781 ccaatagatg attgaacacc cctttatcaa tatttaggaa ggcatctgta aagaaactga
    12841 tgataaccca agacaagaag taagggaaca acatagaagt ttgaaaaatc ttcaccattc
    12901 tcttagaacg gagctcgctg aggataatgg caatccctac agatacaact aaacctagaa
    12961 agataaagcc aagattgtag aggacagtat ttcgtgtgat aataaaggcg tctcttgaac
    13021 taaataagaa tctgaaatta tcgagtccga cccatttact atttatgata ctatctatga
    13081 aaccattact ggtcatgtgg tagtctttga aggcaaccac gttcccaaat actggaatgt
    13141 aaaagaatag aatcaaccag agtgcccctg gcaaaaccat caagagaaag atccagttgt
    13201 ctctcaatgt ttttgaaaac tttttcataa tttcctccct ttttattttg atatccatct
    13261 aaaaattctt ttttagactt ttgataacga ttacattatt agtatactcc tatttgcagg
    13321 ttaggttaaa ctcctaatta tagaaaaaac tccacaaatt atgtagcaga tttaaaactt
    13381 tatcaccact atcaaacaaa tgtcctaaat caattgttta ttttatctct attagcccag
    13441 tgatggcgtc actctgttat aagcatccaa caacggggta tactgaaaaa tctccagact
    13501 agggaactca gcgatagttc ctaatctgga gatttttaat atgttattag gcgtttgctt
    13561 tcaacttagc aataacctct ttaagattat caatcaactc tgcttcagta tgctcagagc
    13621 ctttttcatc tgccaagaac aaaactgctt tttgaagttc ttcttgagag ttttcaagga
    13681 catccttatc tgctgtttct ttgcccgctg tttgtcctcc atccttagtt gctgactggc
    13741 tttccttaat ggactctagg gaagcaatgg catctttgac tgtttgcaag atatcacgta
    13801 aaccttgctc tgtcaaacta tcatctgcaa aagctttctt agcctctgcc aaaaccagac
    13861 gtgctgaatc tgtggtagga ttcgatacac ctgtcaatga tctcaaaaga ttttctaaag
    13921 tttgagtctg cttactaata ctagactaaa atcaaaaagc attatataac agtgatatga
    13981 aatcaactaa agaagaaatc caaac
//
LOCUS       DAVBCG010000042        13844 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBCG010000042 DAVBCG010000000
VERSION     DAVBCG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.042"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            182..658
                     /locus_tag="VQ495_001043"
     CDS             182..658
                     /locus_tag="VQ495_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531015.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1332..1724)
                     /locus_tag="VQ495_001044"
     CDS             complement(1332..1724)
                     /locus_tag="VQ495_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2531016.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1702..2163)
                     /locus_tag="VQ495_001045"
     CDS             complement(1702..2163)
                     /locus_tag="VQ495_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2531017.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDDKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2126..2386)
                     /locus_tag="VQ495_001046"
     CDS             complement(2126..2386)
                     /locus_tag="VQ495_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531018.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2391..2819)
                     /locus_tag="VQ495_001047"
     CDS             complement(2391..2819)
                     /locus_tag="VQ495_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2531019.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2788..3114)
                     /locus_tag="VQ495_001048"
     CDS             complement(2788..3114)
                     /locus_tag="VQ495_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2531020.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3116..4132)
                     /locus_tag="VQ495_001049"
     CDS             complement(3116..4132)
                     /locus_tag="VQ495_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2531021.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4080..5021)
                     /locus_tag="VQ495_001050"
     CDS             complement(4080..5021)
                     /locus_tag="VQ495_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2531022.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5097..5462)
                     /locus_tag="VQ495_001051"
     CDS             complement(5097..5462)
                     /locus_tag="VQ495_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2531023.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5613..6671)
                     /locus_tag="VQ495_001052"
     CDS             complement(5613..6671)
                     /locus_tag="VQ495_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2531024.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6834..7985)
                     /gene="nagA"
                     /locus_tag="VQ495_001053"
     CDS             complement(6834..7985)
                     /gene="nagA"
                     /locus_tag="VQ495_001053"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2531025.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8138..9955)
                     /locus_tag="VQ495_001054"
     CDS             complement(8138..9955)
                     /locus_tag="VQ495_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2531026.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10056..11198)
                     /gene="tgt"
                     /locus_tag="VQ495_001055"
     CDS             complement(10056..11198)
                     /gene="tgt"
                     /locus_tag="VQ495_001055"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2531027.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11328..12185
                     /locus_tag="VQ495_001056"
     CDS             11328..12185
                     /locus_tag="VQ495_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2531028.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12213..12857)
                     /gene="pcp"
                     /locus_tag="VQ495_001057"
     CDS             complement(12213..12857)
                     /gene="pcp"
                     /locus_tag="VQ495_001057"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2531029.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTLERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(12964..13332)
                     /locus_tag="VQ495_001058"
     CDS             complement(12964..13332)
                     /locus_tag="VQ495_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2531030.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13343..13768)
                     /locus_tag="VQ495_001059"
     CDS             complement(13343..13768)
                     /locus_tag="VQ495_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2531031.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
BASE COUNT         4114 a         3102 c         2428 g         4200 t
ORIGIN      
        1 tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaata atgaaaataa
       61 aggactaaag caataactct aaatacatct gatttttata ggatatttca taaaaaaaat
      121 ctacatacct agagtcctat ttagttatgt atcttacata aagagaaagg aattaattat
      181 catgaaatta tctttgtata gtgaagttga acattaccat tttttaagtt cttattcttt
      241 aaatgatatt acatatactg ataccccaaa aaatattatt gaaaagtcta aaaaagataa
      301 gtatagatac ccaatagtaa tacttaataa agaccataaa gtaattggat ttttttgcct
      361 acacctaaac actggtccta aagagtatgg ctattatcaa aatgattatg cgcttattag
      421 aggattttct attcatgata atttcagaaa tcaaggatat ggttctaatg ctctgaatga
      481 aatatttgaa ttcatagact caactttaaa attagaaatt aaccatatta tattggctgt
      541 aaatgaaaaa aatatattgg ctcaaaaagc atatagaaat tccggttttt ctgttgtgaa
      601 aaaagattta gaaggaaaaa gggggaaatt aattattatg gagaaaagca gaaactagta
      661 atgatactag tttctgcttt tctctaagtc tgctataaat ttataacatg ttgaaataag
      721 atgtgaataa attgattgca gaattcaaac caatttctaa caatgtttta gaaaactcgg
      781 cggactattc cagattcaat ccactatatt ctatcttatt tgcatatttt gagagaaata
      841 atctatatac taaactttta tgtttataat tcattccttt cgtttcactc aaggcacaag
      901 acagaatgaa aaagtggtgt gctctttatt ttgttttata ataatagtga gaaaacctat
      961 cactactaca aatcacgggg aggtgaataa gtgagtggta tagccactac ctcgcatttt
     1021 tataggtgtc attctttcca tcaagcacaa gaactgtgac taggagcacg taaacttata
     1081 attgaataag cgactcgttt gtgaaaggaa agaatagtca agggataggc agactcaaat
     1141 ttggtgaagg gaaacccctc atgttaaaag ccaaacttca attttaaaaa ttttaaaatt
     1201 tgtttcgtat tcactactcc ttgcctttat attggattga ttaagagaac ctttcgcgtt
     1261 tctagaaact acagtggact attctggatt caacgcatta tagttgaatt gactaggaat
     1321 tctctttctt ttcaggcttc tcttctgatt tttttctccg ctttttcgct tgaatcagtc
     1381 gctacctctt cctttttatc cgttttctct tctttgattt tgactgaagg aaacagaaac
     1441 tcatattgac tcttatccgt acgaacagtc gtcaccaagc ttgttttctt gttttgatag
     1501 ctcacctgac ctagattaaa aacctgttcc ccactttctt gctcggcttt atccttggtt
     1561 cgtttagcca tggcaaaagc aaccaatttt tctttattta aagcatagtc ttgatagtgg
     1621 gcgatttgtc gggtcaaata aaattgcaac aaaagactaa agatggctgc tatggtgact
     1681 gcgtagagga gaacacctgc cttaactttc ttctttttcc acacgataga tgaactccct
     1741 ttctaagcct ttttgaaact ggaaacgaaa atgaaccagt tgattgtcct ctgtaatctg
     1801 agctgatttg aggccataaa ccataggctg atacccccgt ccactagcat cggttttacg
     1861 aaaatcgtct gactttgact taccgatggc gatgtccttg tcatcttgct tcatgtatag
     1921 gcgattgcct tctacttttt cgaactgcga acggtctaat tctgcctcaa gttggcccac
     1981 aaacaagagc cactcctttt gctcgctttg ttgctggtag cgaacttctg aaatgaggag
     2041 ctgactcata gcttgaaaaa ggagtaatcc cccactgatg acaatgaggg caagcaggga
     2101 ttctaacaag gtaaaagcct tgaccttatg gctctttgat tgccaacaac tgttctgaac
     2161 catggtagac ctccaatccc ttttcactag aaaatacctg aatctcaact ccgttgatgc
     2221 ttacctgatt ttgccccgtc tgcaaggcca tcttagctac cctcaagact tcttcctttt
     2281 gcaagatttt tgcttcctct tgcctatttt tttgaatttg tcccaaaagg agggtcgcaa
     2341 tgctggcaaa gatagctaga gcgactactg cttccagtaa aatcactgcc ctaatttttt
     2401 gtttccttaa tgcgtttaat ttttccattt cctagatata attgatagcg aatcgctcct
     2461 ttactggtct gaaattcaac cttagccagg gacgaattgc ccccagctcg gtcaaatgta
     2521 atactttggc ctgatggtgc ctgaattcct ttaggaactg tcaacttttg actgccattg
     2581 ctaatcatct gcccatccaa gttcagacta gtcttttgtt gactagctac actgcgtttt
     2641 tgggtttccc gatagagttc ttcaaactcc ataaagaaaa tctgttcctc taccgctgca
     2701 aaagtggact ggacagagcc ggacaagccc aaggcaagga tactcacaag tcccaaaacc
     2761 aagagacttt ccagcatggt aaaggcctta atcattgact ttacgatttg cccctccatt
     2821 tttagtatag tattcattat aagctttaac ctgttcttcc gtgatgcgcc catctccttg
     2881 taacttgctc agagtagcat cttcattttt atccaagcta taaagttctg cctggctttc
     2941 caccacctta acaacagctg cttttccttt gtcattgact gcttcttttt gcttggtcag
     3001 attaggtaca aagagcaaga aaagcacgct gataatcagc aagaccacca acatctccac
     3061 caatgtaaaa gctttaacct tagctttttt caagaatgtc atcatttttt tcattttaaa
     3121 aatttacctc catattttga tacatgggca tgagcattgc cgcataaagt aaaacgataa
     3181 tcagtgccac aaagataaaa accagtggct gcaccaaatt catggtgcgg ttgactcggg
     3241 taaaaaaggc ttcccaagtt ttttcagcat agatttccaa ctcactaccc agcttggact
     3301 taacttcccc atactctatg atgagactca attccttcct aaagaaagga taggttccta
     3361 tcgtctgaga aaattcacgg ccatttttca gggtttgagc cagatcttga ccgatttctt
     3421 taaagagctg ggaaccttgt tcctgcatca tttgaaaaat ctgcgtcagc tccattccct
     3481 gtgaaatcat attcccccat tcacgtgcat aataggctgt caagtaggtc tgcacaaaga
     3541 ttccaataaa gggaaggcgt gctaagatag aaaagacact catcttagaa cttcttttat
     3601 aaaaagttag tgctaaaagg gcaagcacgg aaacaagccc taccatgcct agaaaaattt
     3661 gtggcagatt accgataatt cgggtggcaa tattgctact atccagttgt gggagcaggt
     3721 aattccgtag ccccagcata attaagagaa gaaaatccag caaaatcaaa ggataggtcg
     3781 ctacttcaat taactttttc ttgaccttgg ccagattgtc tagatattct tctatctttc
     3841 ccaaactcag gtgaagattg ccatgaactt cagctagaga taactgagtg acaatggtac
     3901 ttgaacatcc caaactttcc atcatttctg agaatgattt cccctgagac aagcctgtac
     3961 gcatctgggt cacacactgc ttgtccaaca aggaactcct atctaaaaag gagatagtct
     4021 ccaccagatg aaaaccgcta gaaaagagat tgttaaatag ggtgatgata tttttttgct
     4081 tagctgtagc taattttttc cgtctcagcc tgaagacttg tgatatgtcc atctttaaga
     4141 agttggtcaa tttgctcatt ccacttggct gcttggtgtt cttgataatc tctgtttgca
     4201 aagtcaacga ttcctcctcc cccgattaat ctctggtagc agactccttg cagaacaact
     4261 gccaattctt cttcactcac acccaactcc agcagacgct cataaacacc tcggatactc
     4321 ttggcgtgaa tggttgaaaa gactgtcgca cctgtcaaac tagctctgac cactgcacgc
     4381 gccgtctcgc tgtcacgaat ttctccgata atcaagagat ctggtcgatg acgcaaggaa
     4441 agtttgatta gattttcata ggttaggccg attgcttcgt tcaactgcaa ctgaagcatg
     4501 tcgtcctgct tgatttcgac aggatcttcg atggacataa cttgctgtcc tttaaagagt
     4561 gacttggaca attcatgcat caaggtcgtc ttaccactcc caaccgggcc agcaaaaaga
     4621 tagagtcccc gttgcctgta ctgcttgcct aattcttcaa tatcctgaaa ccaaaaatgc
     4681 aagtcctgct cctcatcgtg caacaaacgg ataaccaaac tctcatgccc ccgataatcg
     4741 cctacagtag ataaacgtag agacgccatc ttctggtcat aggcataatc acaggaaccc
     4801 agttgactac gtcttttttc tcccacattc atacccgcca caaacttaaa gtgactgata
     4861 acggctgcaa acttttcaaa atcatagcta ccaattttac agcgctcgtc tcctaccctc
     4921 atatgaagct cataggcgtc taacttaggg acaaaataaa tatcctgcgt cccttttttc
     4981 cgagctgaac gaatgatttc ttgtgcaatt tcttgaacca tacttacctc ctcacctata
     5041 ctattcgcaa agcgttagaa aaataaaaaa agcaactcca aaaaagttac ttttctctat
     5101 tttaatttca tacggcaaga acgattcctt tcctgactgg tttgtttttc atagcctgag
     5161 cgtagttttt cgtctgggat aacctgctca tcctgcaaaa gagccaaaga atggtattgt
     5221 tgtaaatact catcacattc atcacaccag cgttggtctt ccgggtccca aaaaatggtc
     5281 atcaagtcgc ttctactttt ataaagaggg gattcttgtt ccaatctaaa ccagcaagtt
     5341 ttgtagtatt tgagagcatc ataatactgg tcaaattttc tacttgctac aatatcttct
     5401 tcccaacctt ctaagaacca ccacggttca aaatctccgt acatttctat aacacgatac
     5461 atattcattc attcctttgt accgtatatt ataaccaatt ccgccagaca aggaaagaaa
     5521 tatgctcatt tcttaatttt ttgataaaaa atccagccat ctgatggatg actgaatttc
     5581 tatttgctta tgtacaaaaa tatttttact acttaggctt ctgagatatc gttttcgata
     5641 atgaccttaa tggcatggtg gtctgctgcc ttactgaaga cttcgtaggc tttttcaatt
     5701 tcactgagtt tgaaatagtg agttaccaat ttttccggtt caatcttatg actttcaagt
     5761 gctttcaaca attgtggagt cgtatttgta gataccaaac cagttgttac attgatgttg
     5821 cgaatccaaa gtttatctaa atcgaattca actggtttac catgcacacc acagttggca
     5881 accgttccgt ctacaccgat aatcttttga cagaaatcaa atgtttcagg aatccctaca
     5941 gcttcgatag cgacatccac accacgacca tctgtcaaat cataaatttc tttaatggct
     6001 tttttagggt ctgaagaatt aaccttatga gtcgcaccga atgatagggc agtttccaag
     6061 cggttatcgt ctaggtctac cataatcaat ttagctggtg aatagaattg ggctgttaaa
     6121 agagcagcca atccaactgg acctgaacca ataatggcta cgctgcaacc aggttctact
     6181 ttccctttta agacaccaat ttcatatcca gtaggcagaa tgtctgacag cataaccaaa
     6241 gcttcatctg acaagtcttc tggagtatgg taaagagtat tatctgcatg agggacacgt
     6301 agatattcag cctgcatacc atcaatcaag tgaccgaaaa tccagccccc ttcgtcttca
     6361 cagtgggcat aaattccttt tttacagtag tagcatttac cacaggcaca gacgcaagaa
     6421 atcaagacct tgtcaccttt tttgaagttg gaaactcctt ccccaacttc ttcaacaatc
     6481 ccaatccctt cgtggccaag aatggtacca ctttggcaag taggaacatc ccctttgata
     6541 atatggaggt ctgttccaca aatagtggtt tttacaatac gcacaatagc gtctgttggc
     6601 ttgcgaataa ctggtttgtc tacatcaaca aaagaagcaa gtcctggttt aacataagta
     6661 taggctttca taaagcactc ctccgttttt ttatttgtaa tatttatagt tcaattgtaa
     6721 gcctttttct ttgtttcttc aagctttttt tgtgattttt ttaacttttt atttaccagt
     6781 aaagtaccct gtataaaaaa gcagaagcta gtctctaact tctgccttct ctcttatgct
     6841 tgataacgtt tcacaccatc taggtaggtt gctaccaatt ccaaatcttt atctaatacg
     6901 atgaagtcag cgtcgtagcc ttcacggatt tggccacaga cgtcatcgat gtgaacggat
     6961 ttagctgggt tgaagctggc catcatgact gcttcatgcg gattcgcaat tccccattcg
     7021 accacattct tcaaaccatc tttgagtttg aggatagaac ctgccaagtt acctgtcgat
     7081 ttgaggcgtg cagttccatt tgcaacgaca actgggaatt ctcccaacat ataatctccg
     7141 tcttccaatc ccccagctgt catacagtct gtgataagag cgatgttttc tgttcctttt
     7201 tgtttgataa gaatttcgca agcctttgga tctacgtggt gaccatcaca gatcaactct
     7261 gcataggtat gtggcaattg gtacatggct ccaaccatac ccaattcacg gtgagtcaac
     7321 ccacgcattc cattgtaggc atgcacccaa acactcgctc cagcatcgac tgcttttttg
     7381 gcttcatcaa aagtcgcgtt tgaatgtcca agagcaaccg tcacaccttc gcccgtaact
     7441 gtacgaacaa agtcttccac cccatcacgt tctggtgcaa gggcaatttt attaagcaag
     7501 ccatttgccg ctttttgcca agaatgaaac tcctcaacac ccgggtctct catataagtt
     7561 ggattttgtg cccccttaaa agtttctgtg aaatatggac cttcataata aatcccacga
     7621 atcttagcac ctgttgcttc tttataatgg tttccaagat tttcagtgac tgcaagcaat
     7681 tgctcataag tggctgttaa agttgtgggt aagaaactgg taacaccggt actaagaagt
     7741 ccttcactca tagtatgcaa tgtaccttca atgttgttgt ccatcacatc tacacctgca
     7801 tatccatgaa tatgagtatc cacaagtcct ggggcaatgc tataacctgt atagtcaatc
     7861 acctcagccc cttcaggaat ctgctctaca tgtttcccaa acttgccgtc cacaagttcc
     7921 aagtaaccac ctcgacgaac tccgtgtggg tagaaaaact gatccgcttt aatatagtta
     7981 ggcataatgt taacctcctt aaaagattga ttctacaatt tattatgtca attacattat
     8041 aaacctttct tttttatttg taaatggtat agacctattt tttggagata ttttaaaaga
     8101 gctggatttc tccaactctt ctctttttta atacaagtta ttttgattta accggcttgt
     8161 cttgagctgt ctgcaaagct gtggcaatcg tatctgcata caattttgct cctgcttcga
     8221 tagtgctact ctcactcccg aaatgaacct ggtctgttcc agcccaaatt tctggatgct
     8281 ctttcgcaac ttgattccaa tctgctatcg taatgtaagg tgtcttctct gccaattctc
     8341 tcatataggc agcagccttc tcaacgatgg cataggtctc ttttgtctta tctccctcat
     8401 aaggagtcac caaaatcata tggtgtccct taggaagatt tttcacgata ctgtcccagt
     8461 catccttgta attctcagga ttatttaccc cagtcgcaat gaccaccgtc ttaggtaaaa
     8521 atttattctg gctattattt agcatgattt catttgcggt cttggttgtt acgctgacct
     8581 gcgcgttaat ctgtgctcca ggaagagctg tctgtagtgc tgtatttgcc cttaaagcca
     8641 ctgagtcacc aattaacata gtgccatcag caattcccaa actgtttgca tctgcccgtt
     8701 ctgccatcac cttggtctgg ccaatatttg ttgcagcttg cttcaagcca ttgacagtca
     8761 agtctgtctc aaacgctccc acttgtggtg ccaacaaggt caccatgcag acaatgaagg
     8821 tcaagattcc tgtacctgct gcaagaattg cgtgaatata aggcagggga cgaagagttt
     8881 gtaaaatagg tgtgttcttg cctgcaatcc aaggttccaa tacataaaat gacagactgg
     8941 caaagccata agaacaaatc agagtcagta atacagcaag aagatttgat gtcaactgtg
     9001 agaaaatgat atagaaaggc caatggaaaa gataaaccgc atagctagta tccgctaaaa
     9061 agctgataat cttcggctcc tgtatatgat gtgtcttttc atgtaagaca cgcgccgcca
     9121 gaatcatggc aagagctgca agactggcaa gcaagaagcc gataagatag gcaaaaagat
     9181 aagtgaattt gacaaagaaa gtcaaaagaa ctaggaaacc aaagcctcct ccaaaaacta
     9241 ctaaagtctt tcgtaaatcc cagattttat ccaactgctt gacgagggaa gtcgtctgac
     9301 gaacgcctac aatagttgct aacatacttc ccaaaaagaa tggatagaca tgagttaaac
     9361 tggagaaata aacagaggaa taagaggtca ctagaaaact accaataaac atggagaaga
     9421 aactgatcaa gaaggcagta gcagataaga gaaagatcat ccccttcaac tgaccatttg
     9481 atttagcttg tttggataag aaccaaactg ccaatcccca aagaatatag tagtgaacct
     9541 caactgccaa actccaatta tgaacaaaca aatgaggaat gaactgagat tcataactcc
     9601 cacctgttaa gagttcatag aagttggtca taaagcctaa gacgcccgca atctggccac
     9661 caattccagc aacatagtct tggcgaacca agaaagtaaa aggcatggtc accaagacca
     9721 tcaaaaccac aggtggcaca atccgataaa agcgtcttct aaaaaatcct atcaaatcaa
     9781 tctcattgtt cttagaaaat tcttcaatga gtagagctgt aatcaggaaa cctgaaaatg
     9841 tgaaaaagac atctaccccg aaaaatcctc caggaaagat cgtctgaaag aagtgataca
     9901 agagtaccaa aagtaaacct ataatcctaa tcaaggaaaa ccatttaatg cgcatacgag
     9961 tttattcttc ctttcattgt acactttatt ctatcaaaaa aagaagaaaa atcctaagag
    10021 aaaacttagg atttttagct tatatcaatt ccattttaga aattacgtcc tgacttatta
    10081 tagccatatt tttccacaaa atacttacgg aattccaaga gattgtcatc catgatggct
    10141 tgtcgcactt gcttcatcag gttaagcaag aagtaaagat tgtggtagct agtcaagcgg
    10201 ataccaaagg tttcatcagc cttgagcagg tgacgaaggt aagcgcgtgt atagttatta
    10261 catgtgtagc aatcacactc aggatccagt ggcgtaaagt cctcagcaaa ctgggcattt
    10321 ttcacaacca aacgtccttg actggtcata caagtcccgt tacgagcaat tcgagtcggt
    10381 aagacacagt caaacatatc caccccacga atgaccccat cgatcaagct atctggcgct
    10441 cccacaccca tcagataacg aggtttattt tcaggcagca gttgagttgt aaagtccaag
    10501 accgcattca tctcttcatg ggtttctccc actgccaaac caccgataga gtagcctgag
    10561 aaatccatgc tgacaagatc gtgagctgat tggcggcgaa ggtcttcaaa tcctgcacct
    10621 tgcacaattc caaacaaacc ttggtcatgt ggacgacggt gagccttcaa accacgctca
    10681 gcccaacggc tggtacgctc gatcgatttc ttaacgtagt cataaggttg ataaaactga
    10741 ggacattcat caaaggacat catgatgtct gaacccagat tattctgaat agagatggct
    10801 ttttctgggg ataggagcat cttagaacca tttagatgat ttttaaaggt tactccttct
    10861 tctgtgatat tacggctatc tgctaaagaa taaacctgaa aaccaccact atctgtcaag
    10921 ataggctggt cccaattcat gaacttgtgg agaccaccag cgcgtgcaat gagttcatct
    10981 ccagggcgaa gccagagatg ataggtgttt gataggataa ttcccgaacc catctccttc
    11041 aattcttcag gtgactgagt tttgacagtg gcttgtgtcc caactggcat aaacataggt
    11101 gtcggaaagg taccgtgggg agtgatgatt tctcctagac gagctcctgt gtgtttttct
    11161 ttcttaatca aacgatattt gattggtgaa tctgacattt tttacctccg aagctgggaa
    11221 agacagtccc agttcataca gtgtgcccaa ggacacactg tatgatttta tcaaaaaaag
    11281 taggaactgt cacgaactat gtataagaac tatggtataa taagagaatg aactatccaa
    11341 aaattgattt aaaaacaatt cgtcaggagt caaaacattt tcaggctgac accccccgtc
    11401 tctttctcct ctatatcctt cccagtatgc tggtcatctt atctggtttt cttaatccct
    11461 tatctcggat ccacgggact gttttagaac agccattttt cagcatactt ggccaaatac
    11521 ttcaaaccta cctctttcct ctactagtct cctttatcgg gactattctt ttgaccagtt
    11581 ccgtctatgc aacactgaca ctcatgaaag attctaaaac ggagccatct gtaaaaaaca
    11641 gcctagctct ctttgatgag gagcgctttt ctcaaacctt tttgacccta cttctcaaac
    11701 gtttctatct ctttttatgg agtatcccta atttacttgg gatttacctc ctcttttata
    11761 gtagcttttt agccaaaaaa ttcgtcaccc tacatcctga gtttccaaat ctagatctct
    11821 catcagttga aaccgagcgt ttcctcatgg tctttggact ctactttctt gcaagtctca
    11881 tcttgatcat tgtaggaaat atcctctata ttccacagta ttatgcctat tcacaggtgg
    11941 aatttcttct ctgttatagt ctagacctag gacaggtccc accaagacga atcctaaaaa
    12001 ctagccgttc ctttatgaag ggctacaagt tccagcactt tgtcctagat ttacagcttc
    12061 ttccttggta cttcctcaat tggattacct ttggcattgc tagtttttca ctcctacctt
    12121 atatccaatg cactaaaatt atgttttacc gagcagtatt ggctcgaaaa cgtccaaaag
    12181 cttgaaggtt tttcccctca agcttttttc tatcaatgag tttctccgcc taccaacttg
    12241 agttcctgat ctccatgttc tatcatagcg ccgattgctg cttctatccc tcgccgaata
    12301 tccactaaac tcatagttgg agtagtcggt ctgttcacca cctgttccat catataagga
    12361 atatgcataa aacctgcctt aacatatgga aatttctttt ctaccaaata gagagcctga
    12421 tacatcaaat ggctgcagac aaaagtccct gccgtattgg aaacagaggc cggtaagccc
    12481 tcttttttta tagcttgaac catcgcttta atcggcaaac tactaaagta ggccgaagca
    12541 ccatctgggc gaatgggacg gtcaatcggt tgattatctt cgttatcaga aatgcgtgca
    12601 tcgtcttgat taatggccac tcgttcaagt gtcacactag ttcttccacc agcttgccca
    12661 atacaaagga caaagtcagg ttgataacga ttcatctctt cttccaatac ttgagcagat
    12721 ttgtgaaaaa ctgtcggcac ctctagccaa cggacctcag caccatggat ttcagctggt
    12781 aaacctttaa tggcctccaa agttggattg cccttttccc ctccaaaggg ctcaaaacct
    12841 gtcactaata ctttcatttc taactcctca aaaccaattc aatgagactt ttttcttaaa
    12901 aacaagtata acatatttcc cttattctgg attaaaaagg attagcgaga ttaaaatcga
    12961 ccttcaagtg tatttaaaga gtaaaatact aatcaaggcc aaaatagctg atccaccttg
    13021 cttcaaaata atttttttat ccgctgttaa agagccgtaa gtcgcagcac caatcacaaa
    13081 taagacaaaa atagtcacaa tttctaaatt ctgtgagaaa taaatgccat accagagaaa
    13141 gactcctagc agagccttat aaatcccttg atttttgaac aatgaactta ctgacggatg
    13201 agccaattct tccttttcca tattaaatac acgactagtc gcatctgatc gcgtagcaat
    13261 actttccaaa taaaaaatgt aaaaatgctc caaagcaacg atcgttgtta aaataattgt
    13321 aataattgac atctatttct ccttaaattt ctatattttc aatacccaaa accaatttat
    13381 ttaataaacg gctgagttct gttctctcaa actctgttaa gatactttcc atctcttcct
    13441 taaccttgat atgttgcaga gggggattca ccactaactg ctccttggca tacttcgcag
    13501 cctctaccaa cacttcccgc tgattttcag gattacgatg acgctccacc aaaccttccg
    13561 tttccaaaat cttgaaatgc cgtgtcaaag cagcctgatc aattttcaaa cgctcctgaa
    13621 ccgccatttg gttacaagga gaatgctcca gcaaaaacag taaaatctga taccgtgtca
    13681 aactaatccc aagccttttt tcaaacaatt gcgtactcgc ttgctcagac aagcggagtt
    13741 gatacagtaa atcttgaatc tctatcattt cttcctccta tccattgact tatcaatagt
    13801 tgaccaatca attataatat aaagtatagt gaaatgaaat aaga
//
LOCUS       DAVBCG010000043        13373 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBCG010000043 DAVBCG010000000
VERSION     DAVBCG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13373
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.043"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(48..2480)
                     /locus_tag="VQ495_001060"
     CDS             complement(48..2480)
                     /locus_tag="VQ495_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2531032.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(2482..2940)
                     /locus_tag="VQ495_001061"
     CDS             complement(2482..2940)
                     /locus_tag="VQ495_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2531033.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(3059..3787)
                     /locus_tag="VQ495_001062"
     CDS             complement(3059..3787)
                     /locus_tag="VQ495_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531034.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(3787..4794)
                     /locus_tag="VQ495_001063"
     CDS             complement(3787..4794)
                     /locus_tag="VQ495_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2531035.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(4832..5590)
                     /locus_tag="VQ495_001064"
     CDS             complement(4832..5590)
                     /locus_tag="VQ495_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531036.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            complement(5553..5843)
                     /locus_tag="VQ495_001065"
     CDS             complement(5553..5843)
                     /locus_tag="VQ495_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2531037.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(5914..6016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6099..7445)
                     /gene="cbpD"
                     /locus_tag="VQ495_001066"
     CDS             complement(6099..7445)
                     /gene="cbpD"
                     /locus_tag="VQ495_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2531038.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(7534..7800)
                     /locus_tag="VQ495_001067"
     CDS             complement(7534..7800)
                     /locus_tag="VQ495_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531039.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(7802..9154)
                     /gene="dnaB"
                     /locus_tag="VQ495_001068"
     CDS             complement(7802..9154)
                     /gene="dnaB"
                     /locus_tag="VQ495_001068"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2531040.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(9198..9650)
                     /gene="rplI"
                     /locus_tag="VQ495_001069"
     CDS             complement(9198..9650)
                     /gene="rplI"
                     /locus_tag="VQ495_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2531041.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(9647..11626)
                     /locus_tag="VQ495_001070"
     CDS             complement(9647..11626)
                     /locus_tag="VQ495_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2531042.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDV
                     SREQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMF
                     SRRVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDE
                     IGKVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISD
                     KIRSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFI
                     EKEGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQD
                     FPDNTVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRV
                     TSRTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTK
                     CYDTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGH
                     FNLAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(11762..12310)
                     /gene="raiA"
                     /locus_tag="VQ495_001071"
     CDS             complement(11762..12310)
                     /gene="raiA"
                     /locus_tag="VQ495_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2531043.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(12390..13052)
                     /locus_tag="VQ495_001072"
     CDS             complement(12390..13052)
                     /locus_tag="VQ495_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2531044.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(13049..>13373)
                     /locus_tag="VQ495_001073"
     CDS             complement(13049..>13373)
                     /locus_tag="VQ495_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA helicase"
                     /protein_id="HET2531045.1"
                     /translation="PNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPC
                     VDVFVVEANHRLFTKSSLIQIGGRVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMN
                     KEAGL"
BASE COUNT         3896 a         3193 c         2196 g         4088 t
ORIGIN      
        1 tttcactcca ctgtgactta tggtcagatg ctgatacttt tattctttta tgcaatatca
       61 aattttaact ggcctgcttt gacaccaatc ttaagtgtgc tgcctgccac taaatctccc
      121 ttaagaagaa gttctgccaa cttgtcctcc acttctgttt gcagggttct gcgaagtggg
      181 cgagctccca tctctgggtc atatccttga tttgctaaca atttcagagc tgaagcttgt
      241 aatttcaagt caatgccttt ttcagccaaa cttgccacta aaggcttgac cataatcttc
      301 accacttcct gcatatggtc actagatagg ctatggaaga ccaccttctc atcaatacgg
      361 ttgatgaatt ccggtctata agcttttttc agttcttcaa acatgcgttt ttccatattt
      421 tcctggtcaa aacgaatatc cttagcccca aaaccaacag tcttatcatc acgaagggca
      481 gtcgcaccta ggttcgatgt cataatgata atggtatttg aaaaatcgac cttgcgtccc
      541 ttgctatctg tcaaaacacc gtcatccaga acctgcaaga gaacattaaa aatatctggg
      601 tgggccttct ctacctcatc aaagaggaga acggaatagg gtttattgcg aaccttctct
      661 gtcaactctc caccttcttc gtaacccaca tagcctggag gagctccatt tagacggctg
      721 gctgcgaatt tctccatata ctcactcata tcaaagcgga taagggctga ttcgtcatca
      781 aagagaactt ctgccaaagc cttggccaac tcggtcttac cgacacctgt gggtcctagg
      841 aacataaagg aaccgatcgg acgcttgtga ctgcgaattc ctgactgatt acggcgaatc
      901 gcacgactaa tgcttgaaac agcttgatct tgaccgatga cacgtttatg gagttctgct
      961 tcaagattta gatacttctt agcatccgtt tgagtcagtt tttgaactgg gattcctgac
     1021 aagcgactca aggtggtcaa aatatcagac tctgtcacca agtctttgta gacaggtact
     1081 tcctcttctt ttgcgattag ctgggctgcc tgtttccact tgccatccat cagggcctta
     1141 tcagctggac tcaaatctga atcgtctgct tttacatact ttaccttatt ttgcactgtt
     1201 gctgctgctt cgtccaagag gtcgatagca gagtctggca agtgacgact ggttaaataa
     1261 cgatgagcca tcttaaccgc tgtttcaacc gcttcatctg tgatttgtac acggtgatgt
     1321 ttctcataag tcgccttcaa accttgtaaa atagtcatac tatctgccac acttggttct
     1381 tcaatcgtca ctttagcgaa acgacgagaa agagccgcat ctttttcgat atgtttttga
     1441 tattcttcct gagtggtggc accaaccgtt ctcaaagctc cacgcgccaa ggctggtttc
     1501 aagatattgg ccgcatccag agtcgaatca atcccgctac cagaacccat gatggtgtgg
     1561 agttcatcga taaagaggat gacttggcca tcttcttcaa tatccttgat gatattattc
     1621 atgcgttctt caaagtcacc acggaagcgt gtccctgcaa cgacattcat caaatcaagt
     1681 tctaacacgc gcatcttagc catttccgca ggcacatcac cactggcaat acgctgagca
     1741 agaccaagca ccagagctgt tttcccgaca ccagcatccc caaccaagac agggttgttc
     1801 ttagtcttac ggctcaagat ttgaatcata cgtgagattt ccttgtcccg accgatgact
     1861 ggttctaact tgccagaacg cgcttgctct gtcaaatcat gcgtatagtc ctcgagacca
     1921 ccactaggag tctgcggcat gcccatcata ttggccatag aattttgttt gtcagccaca
     1981 gtacgatggc gttggcgtaa agccttgaga tcttcacgag tccagcctgc ccgttcttct
     2041 aaatttcgac gaagagcagc aatcttgacc tgatctttct tgtcttcata agaaaaacca
     2101 gccctctcca agatacgagt cgccaaggca ttgccatcat gcaaaatcgc atagaggacg
     2161 tgctctgtcc ctagtacctt agcatggacc actgacgcta catactctgc ttcatcaaaa
     2221 agaacctgca aacgacggga gaacggcaat tccataaagg tttcatcctg gctatagtcc
     2281 gtttcagtca gttccaaagc cacctcttct aaacggtcca tctcatacgg ataatcattt
     2341 aaagttgccc ctgctacact ataactgtga ttagacatgg caatcaacaa gtgccacgac
     2401 tctagataac gagctccaaa atgtccagca accatgtagg cactttcgat acattcattc
     2461 aatgcttttg aatagttcat cttacttccc ttttctatct acctcttgta tgacctgacg
     2521 tagcatgttt gctcgaacaa ctggagcttc ttctcctaaa acgcgatcca aagctactga
     2581 ttctagcaaa ttcatctcct gcttggtcat caattcctgc tcaaccaaaa gctggagaat
     2641 atcctcataa atttcttgac tgactcgctc accaatcgag taaagcagct cccggagcat
     2701 ttcatgatga ctagaaaact caatccgtcc tatacgaatg tagcctccgc caccacgctt
     2761 actttcaacc aagtagcctc tactttccgt aaagcgtgtc ttgatcacgt agttaatctg
     2821 actaggaaca acctgaaagg tatctgccaa ctgactccgt tgcaactcca cgataccaga
     2881 ttgatctaaa atcgccttga tgtaggcctc aatatgatcc gatgtatttt taaatctcat
     2941 aacaaaccca cctctctctt taaaccttga ccttgtttga ctattatacc gaaattttct
     3001 catttttaaa agaaaaaggg cgctggtaaa ggataatctt caccaactcc ctatttttct
     3061 acttatctaa gcctaattct gccaagattt gacgtttgta ggcaatcttt tggacttcct
     3121 tgtcctcatc ttcagaccaa tctagtttaa tttctgaaac aatctgccca gggcgatttt
     3181 tcaagatata gatacggtcg ctgagattga gggcctcctc aatactatgc gtgatgatca
     3241 gggttgttag ctgcaactgt ttgtgaatct caagatacca agcgtggagt tccatctttg
     3301 tcatctcatc caaggcgcta aaggcctcat ctaagagaaa gagcttgtgc ccaaaaaggt
     3361 aggtccggag taaggctaca cgctggcgca tcccaccgct aagttcatga ggatacttgt
     3421 ctcttacagc tgtcagctgg aaggtcgcaa gaattttatc cgctcgggaa atagcttctg
     3481 ccttatccac cttttgaatc aagaggggca gaatgatatt tccaagcacc gtcttgtgct
     3541 ccaagagcag atccttttgc aacatataac tcacgcgccc cttgggattt tcttcaccat
     3601 caaggacaat tctccctgac tgaacttcta aaatcccagc gattagatta aagagggtgg
     3661 tctttccaac accacttggg cctagaatgg aaaccacttc gcctgaagtc acctgtaggt
     3721 tgatatcctc taaaatcctc tcctgaccat aggcataact gacatgctct agtctaattt
     3781 ctgtcattat ttcacaaatt cgttggtgaa gcctttgtct gtcaagtctt ctttaaggat
     3841 accattttct ttatcccatt tatagaaagc attccagcga gctgcgtcaa attgacccca
     3901 tttttccttg tcgcttgcgt attcttttga caagtatttt tgagattcga tgacaaagtc
     3961 acgtttttcc ttgagttcag gtgcattctt gatgagaata tctgcagctt cttctggatg
     4021 ttccatggca tattggtagc cttttttgat ggcttggatg actttgcgag cttcttcttt
     4081 gttatctttc agatagtcgt tgtttgcgat gataactggt gaatagtagt caaactcctt
     4141 gacatagtct ttcaagtaca tgaagttagc atctacacct tgagatttag caaggatacc
     4201 atcccaaccg tagtaaatcc aagcagtatc aaagacgcca ttggcaatcg gtgtgattga
     4261 gtttgagtcg ttatttggta ctttttcaac cttctcaaag tctccacctt gagattctac
     4321 caaggttttc aacatagcaa gttcagttgg gtcattccat gtcccatatt tcttaccaac
     4381 caagtctttt ggactgctta cattatcaga tttacgagag atgattcctg atgtattgtg
     4441 ttcaacaata gctgcaacgg cagtgattcc tgctcctttt tccaatttct tagccatgta
     4501 gtcttggaaa tacactgcaa atggtgcctt tccgttgata accaagtcag aagaactttc
     4561 ttctggtggc aatttcaaat caacatccac tccagcttct ttgaaataac ctttttcctt
     4621 ggcaacataa agccctgtgt ggttggtatt tggtgtccag tctaggataa agtcaacctt
     4681 cttaagttct gcctctttgt tgtctttaga agcagttcct tgaccacagg ccacaagcac
     4741 aacagctaca agagctgtta caagcgttaa aaacactttc catgttttct tcattttgat
     4801 ttcctctttt ctttttcaaa aacgctctat tttaagtacg tttccattta atcacatatt
     4861 tttcactgat atcgaccagc ttcataccca aaagactgat aatcgacacc agaataataa
     4921 tggcaaacat ggtatcatac tgaaacagtt ttttagactg aatcatataa acaccaagac
     4981 cttcaaaacc tcccaaccac tcagatacca cagttgtgat aaaggcgtag gagacactga
     5041 ccctcagacc tgcataaaag taaggcaggc taaccgggat tttaaaatgc cacaggattt
     5101 gccaagggtt tgctcgcatc agactaaaca aagtcagcat atccttgtca caatgcctaa
     5161 aaccgtccaa aatactaacg atgatgggaa aggttgttgt taagataatc aagacaatct
     5221 tgggcaaaat cccataaccc agccacaaga ccaggatagg agctatggca atggtcggaa
     5281 tggtctgaat gaccaccatc atagggtaaa tcaggtcatt gagccaagtc aaactatcca
     5341 tgagcacagc cataagacag gcaatcaaaa ctcccaaaat cagccccagt aaagccactc
     5401 tcaaggtcgc ccagctatgg tgccagagaa attctctgtc acgaacaaag ggctggagaa
     5461 tttcaagagg tgtcggcagg ataaacttgg ggagaagttt aagaaaacct gctaactgcc
     5521 agattgacaa tactccgaga aagcccaata gactaatgtg tcgtctcagt atacttttca
     5581 agtttctcat caatacttaa aatctctcct acatttattt tgacattggc aaagacattg
     5641 tctgcctcct gccctgccac ttccagcgct tcttttagaa tgcgcataag ctcatcaaac
     5701 tccccttcca agaccgtttc aaatggtgtc actaccatcg tcacttcttg agtttgcaga
     5761 taagcaatga cctgatcaat aacagctatc cgatcaatcc cctgtgctag gggtaaaact
     5821 tgcaaggcaa tgcttgcttt cataagaacc tcctcttctc gttttccttt taaaaaaaag
     5881 aaaaaccttt accatacatg gaaaaggtgc aaaattagac taagtatcgt tccctccgct
     5941 ggcattaccc agatcaggtg cggtcgaagt ttaacacttc ctctcagact gtacacagac
     6001 tcccatatat tatatgtata tatgatagcg caaaataaga aaaatgtcaa ggaaactgct
     6061 tacagaaaag agcaggaaat ttctcctact ccaattttct atactcgttc tccatcactg
     6121 tttactctgt agccatccac cgtcgtatta acagctaagg cgcctgaaga gtaagaataa
     6181 taccacttac cagagacctg gtaccagcct gttttcattg cccctgaact acccagataa
     6241 taccacgagc cattctcctt taaccatcct gtctgcattt caccagataa tttcaaataa
     6301 taccagctag aaccgtcttt cagccatcct gttgatttag aaccatttga tttgaaatga
     6361 taccaactac catttatttt cttccaaccg acagttgaac tattattgga tccatagcta
     6421 gagatatttc ctgattgatt ctgataattt tgtgaagaag tcactccctc tagctgtata
     6481 tagcgacgac ttccattata ggcagtataa ctcaaccact tgtagccgtc tttttcaagt
     6541 atctgatcat aatgaacctt ctccccagga taatagtaat caatcacagt tccgctaact
     6601 aggggttcag ttttgatagc agacttggtc ttaaaataat gagttccacc tgttgaagaa
     6661 agaacagaat tacctagagg atttttattc acagcctcca attgaacata acgatagctt
     6721 ccattatagg cagtataact caaccacttg tagccgtctt tttcgagaat ttgatcataa
     6781 tgaaccttct ctccaggata atagtaatca atcacagttg cactaactag gggttcagtt
     6841 ttaatagcag acttggtctt aaaataatga gttccgcctg ttgaagttga ggattgacta
     6901 ttcccaacac tgccaccatc caaatcttta aaatgaataa atcctgtcat cgtgtttgct
     6961 tttataactc gtttattata ggattctgta taaccatagt tatattcctc aatctcaatc
     7021 tgatctccca ttacatttga cacccaggca acatgaccat aagttcctgc agtagaccaa
     7081 gtaatggaac caatcgtcgg tgtattatct acacgataac cttcccgacg agcacgatgt
     7141 ccccattcat tcgcatttcc ataagctgcc ggaatttcaa aaccattgac attactcaaa
     7201 cgaaaggcta caaaagaagt acactgacga gaatacatgc gccactgatc aatctcctgg
     7261 ctcccatttt tataataagc aggataatca tccccacgcg caatcgatcc atttcctctg
     7321 gaataggcat agacactatc accagctgct agcatcaatc ctactactaa aaaaggaaca
     7381 agctttttaa ctgacatctt caagtaataa cttgttcctc ttgctataaa cggtaaaatt
     7441 ttcattcttc ctccttgaaa aataatataa agcgatgatt gccttcacct aaattattcg
     7501 gagaaaaaag aaaaagtgag aaaatttctc actttagtca agataatgaa tcaaattctt
     7561 ttctgtaaga atatctgagt aagtaaagga ttcaacgtaa acattaactt gtttatctcc
     7621 ttccacatcc ccaaactcca caataaatag agatggataa acttcaatta gcttacccaa
     7681 tctatttttt tggcgcttac gaccattctc caaagtcatt tctacgactt gtccctcatg
     7741 tgccttgatt tcttctttga tttttttcat cttggctaca tctgtaaatg catctgacat
     7801 cttatgcctc cctctttgag atacttgaaa atttattgta ttctttttgg aaaatcaatt
     7861 ccactgttcc acgagctcca ctacggtttt tctcgataat aacttccacc ttattatttg
     7921 ggataccctc ctcttcttca ccaccacgtt catagtagtc atcgcgatag agaaaagcta
     7981 cgatatcagc gtcctgctca atagacccag attcacgaat atcagacaag accggtctct
     8041 tgtcctgacg ttgttctaca ccacgagaaa gctgactcag agcgattact ggaaccttca
     8101 attccttggc tagtattttc aactgacgag aaatttcaga aacttcttgt tgacgatttt
     8161 ctcgaccagt tcccgtgata agttgcaaat agtctatcaa aatcaaacca agatttccag
     8221 tttcttgagc caatttacga gaacgagaac gaatctctgt aatccgaata cctggcgtat
     8281 catcgatata gatactggcg ttagctagat taccctgagc aatagtatat ttttgccact
     8341 cctcatctgt caattgccct gtacggatag aatgtgactc cactaagcct tctgcagcta
     8401 acatacgatc taccaagctt tccgcaccca tttcgagtga aaaaatagca accgttttgt
     8461 ccaacttagt cccaatgttc tgagcgatat tcaaggcaaa tgctgtctta ccaactgctg
     8521 gacgagctgc taagataatc aactcctcct catgaagtcc tgttgtcata tgatccaaat
     8581 cacgataacc tgtcgcaata cctgtaatat cggtcgtttg ttgcgagcga gcttccagat
     8641 ttccaaagtt gagattcaac acatctcgaa tgttcttaaa cccgcttcga tttgcatttt
     8701 cactgacatc aatcagccct ttttctgcct gagcaataat ttcatcagct ggttgtgacg
     8761 cttcgtaagc ttggttgaca gactctgtca acttggcaat taaacgacgt agcattgctt
     8821 tttctgcaac aatcttagca taatactccg cattagcaga agttggcaca gaattaacaa
     8881 tctcaaccaa gtaagacaag ccaccaatat tctgtaaatc accttgatta tcaaggatag
     8941 tacgaaccgt tgttgcatct atggcatcac cacgatcgga taaatcgacc atggcttgga
     9001 aaatcaaacg atgggcatac ttaaaaaagt cccgagactc aatgtattct cgcacaaaaa
     9061 caagtttact ctcatcaata aagatagccc ctaaaacgga ttgctcagct aagatatctt
     9121 gaggttgtac tcgtaactct tctacttctg ccatcagact tcccttcctt ttacaatctt
     9181 gtcaagaagg tgtaaactta tccttctttc acacgaagat tgattacact tgtgatatct
     9241 tgatagattt tcactggcac atcaatcaaa ccaaccgctc gaatcggagc ttgtacttga
     9301 atatgacgtt tatcaatctt aattccaaat tgtttttgca attcttctgc aatcttctta
     9361 ttggtaatag agccaaaggt acgaccatct ggaccaactt tttcaacaaa ttctacaaca
     9421 gtttcttctg cttcaagttg tgctttaatt gcttttcctt ctgcaatcat ctcagcgtga
     9481 gctttttctt ccgatttttg tttaccacga agttcaccta cagcttgagc agtcgcttct
     9541 ttggctagat tctttttgat aagaaagttt tgcgcatacc ctgttggtac ttccttaatt
     9601 tcgccttttt taccttttcc tttaacatct gctaaaaaga ttactttcat tcttctttct
     9661 ccttttcctt tatttcattt aatacaattt ctgtcagttt ttcacctgct tctgacaagg
     9721 ttacatcttt aatttgagct gctgccaaat taaagtggcc tccaccgcct aactcttcca
     9781 taatccgttg tacattcagt ttactacgac ttcgagctga gatagagata aatccttgtg
     9841 tattcttcgc aagaacaaaa ctcgcttcaa tacctgacat ggctaacatg gcatctgctg
     9901 ccttactaat aacaactgta tcatagcatt tcgtgtcctt agcctctgct attagtacat
     9961 ctgaacctaa tttacgcccc tgtaaaataa gttcattgac ctcacgatat tcttcaaaat
    10021 ctgtcgcagc gatttcctgg atagcaatac tatcacttcc gcgcgttctg agatagctag
    10081 caacatcaaa tgtccgacta gttactcgcg aggtgaaatt tttagtatcc aacatcatac
    10141 cagccatcaa gacacttgct tgcatacgac tcaaacgatt tttcttagaa ttctggaact
    10201 gaatcaattc cgttaccaac tcactggcac tacttgcacc actttcgata taagtaatga
    10261 ccgtattatc tggaaaatcc tgatcccttc tatggtggtc aataacaatg gtttgggtaa
    10321 ataaatcata aaattctttt gataatgtta aggctgtctt tgaatggtct acgagaatca
    10381 acaaagaacg attggtcacc atccccattg catccttaac agacaacaac ttcgtaactc
    10441 cttctttttc tatgaatgaa acagctcgtt caatatctgg agacatttgt tcttcatcat
    10501 aaagagcata gctattttca atcacattgc tggcgaacaa ctgcatacct acagcagagc
    10561 ccaaagcatc catgtctaaa tttttgtgac cgactacaaa aacctgatct acactccgaa
    10621 tcttatctga aatagctgtc atcatagcgc gcgtacgagt ccgtgtacgc ttgattgaag
    10681 cagcagaccc accaccaaaa taaactggat ttttcgtttc gtcgttttcc ttaacaacca
    10741 cctgatcgcc accacgtact tcagccaagt tcaaattgag caaagcaact ttccctatct
    10801 catcatgatt tccatcgcca taagaaaatc ccatacttaa ggtcaagggc aactgtctct
    10861 gtttcgactc ttctctgaaa gcatcaataa cagaaaattt atcattcatc aagccctcaa
    10921 gcaccgtgta gtcagtaaat agataaaatc gatccatact tacccgacga gaaaacatca
    10981 tgtatttttc tgaaaactct gatataaaat tagctacaaa actattgatt tgactaatat
    11041 ctgactcaga agttgcatcc tccaaatcat cataattatc cacagagaca atcccaatca
    11101 ctggtctact tgttaccaat tcatctgtta tggcttgttc cctggataca tctacaaaat
    11161 acaaaacacc ggaagaagca tccatatgaa cagcataacg cttctcacca agcttggcat
    11221 aagtagacgg atttcctact gaagctgaag ccttgataat cgtttgaaca gcttctaaat
    11281 caaaatcacc atcttccttg gtcaaaatca attcagcata gggattaaac cactcaacct
    11341 ctccagaaga taaattcaat ttcataacac ctacaggcat ttgttccaat agagatgtca
    11401 aactttcttc cgcttggtgg tttacatact gtatctgttc tacatcactc cttgtataat
    11461 gcactctcag tttcttaaat aaaaaaacat agcctcctac aaaaagaaac aaaattaaaa
    11521 ccgtcaacag attattatta acaaaaataa tgaaagtgga taagactcca aacgcaatca
    11581 atcctactag aataggaaaa attggactta cataaaattt tttcattcaa aacctcttgg
    11641 cacccattat accataatac ccctcaaaaa gcgacttttt aaaagtgtaa tcagtaattc
    11701 tatcaattat aagaaaaagg tagtttacaa ttcagtaaac ctacctttac acatattgaa
    11761 attaagattc tttaacctct aacaaaccaa tttcgccatc ctcacgacga taaatcacat
    11821 tggttgtctg atcttcaaca tccacataga taaagaaatc atgccccaat aaatccattt
    11881 gtagaattgc ttcttccaaa tccattggtt ttaaatcaat ttgttttgaa cgaacaactt
    11941 tagactggac aacatttgaa tcttccacca aagcatctgt aaataattga ccagttgcta
    12001 ccttattttt atttttacgc tcgatttttg ttttattttt acgaatctga cgttcaattt
    12061 tatcagttac aaggtcaatt gaaccataca tatcttgaga tacatcttct gcgcggagag
    12121 taatagatcc aagcggaatc gttacttcca ctttagccgt tttttcacga taaactttta
    12181 agttaattcg ggcatccaac tcttgttctg gttggaagta cttttcgatc ttttcgagtt
    12241 tagaaactac ataatcacga attgcttctg ttacttctag gttttcacca cggatactat
    12301 atttaatcat atgagtacct tctttctaaa catttttgtt tttatgattt tattataacg
    12361 ctttcattct atttttgcaa attttttcct catcttacaa gggaaaatgt ttttacatcc
    12421 ttagcaccag cttcttccaa cagtttctta acacgattta tagttgctcc tgtagtatag
    12481 atatcatcta taagtaggat ttttttagga atagtgactc cacttttaat aaagaaagga
    12541 agttctgtcc ccaagcgctc tgaacgattt ttagaagaac tggctctctc ttctcttttc
    12601 tctaataaat ccagatactc aaagcctgct gcctctacca agccctcaac ctgattaaat
    12661 cctctattag catatctatc aggacttagg ggaattacaa caaattgata ctctttgtac
    12721 tttttcaact cctcacttaa aaatgaagcg aaaacttttc ttaacaggaa gtctccatca
    12781 aacttatacc gactgaaaaa atccttcata gcttgattgt aagtaaaaat cgctctatga
    12841 ctgactccaa ctccttcttt acaccaaaat tgacaatctt gacactttgt tgacaactct
    12901 gttttcatac aatttggaca gttctcttcc ccaattcttt caaaagtaga atcacagtct
    12961 gaacaaagac aagagtcatc attcctcaga agtaagagac tactaaaagt taaaacagtc
    13021 ttcatagtct gcccacataa caagcacttc atagaccagc ctccttattc atcatctgaa
    13081 tttccttaat cgccttcttg attgaagcat ttaacccatc atggaagaaa agcaaatctc
    13141 ctgtcggtct atccatgctt cgtccaactc gtccaccaat ctgaatcaaa ctagacttgg
    13201 taaacaaacg atgattggct tctactacga aaacatccac acaagggaag gtaactccac
    13261 gctccaagat tgtcgtactg ataagtattg tcagttctcc atctcgaaaa gcttgtactt
    13321 gctctaatcg atcctctgtt acagaagata caaagccaat tttctcattt gga
//
LOCUS       DAVBCG010000044        13195 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBCG010000044 DAVBCG010000000
VERSION     DAVBCG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.044"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            256..603
                     /gene="ssrA"
                     /locus_tag="VQ495_001074"
     tmRNA           256..603
                     /gene="ssrA"
                     /locus_tag="VQ495_001074"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(786..873)
                     /locus_tag="VQ495_001075"
     tRNA            complement(786..873)
                     /locus_tag="VQ495_001075"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(837..839),aa:Ser,seq:gga)
     gene            1035..1775
                     /locus_tag="VQ495_001076"
     CDS             1035..1775
                     /locus_tag="VQ495_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2531046.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1772..2683
                     /gene="pfkB"
                     /locus_tag="VQ495_001077"
     CDS             1772..2683
                     /gene="pfkB"
                     /locus_tag="VQ495_001077"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2531047.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2680..4632
                     /locus_tag="VQ495_001078"
     CDS             2680..4632
                     /locus_tag="VQ495_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2531048.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4796..7021
                     /locus_tag="VQ495_001079"
     CDS             4796..7021
                     /locus_tag="VQ495_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2531049.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7066..7494)
                     /locus_tag="VQ495_001080"
     CDS             complement(7066..7494)
                     /locus_tag="VQ495_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531050.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            7632..8774
                     /locus_tag="VQ495_001081"
     CDS             7632..8774
                     /locus_tag="VQ495_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2531051.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8783..9997
                     /gene="thiI"
                     /locus_tag="VQ495_001082"
     CDS             8783..9997
                     /gene="thiI"
                     /locus_tag="VQ495_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2531052.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10132..10956
                     /locus_tag="VQ495_001083"
     CDS             10132..10956
                     /locus_tag="VQ495_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2531053.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            10978..11717
                     /locus_tag="VQ495_001084"
                     /pseudo
     CDS             10978..11717
                     /locus_tag="VQ495_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11730..12896
                     /locus_tag="VQ495_001085"
     CDS             11730..12896
                     /locus_tag="VQ495_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2531054.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
BASE COUNT         3830 a         2524 c         2982 g         3859 t
ORIGIN      
        1 attcgttcta ttcttatttc attttactat attttggtgc aataagtgaa aagtagtccg
       61 aataatataa ggattgattt tatagttttt aaactcaaat gaattgaaat aaagagagta
      121 cgaaaattct catctgaaag tattttagaa taattctctt cgtgaatttc ttcaaaacag
      181 atagcttcat cttaggtatg tgatttcttt ttgcattttt gagttagata aggtataatg
      241 gttttattgt cttttggggt cgttacggat tcgacaggca ttatgaggca tattttgcga
      301 ctcgtgtggc gacgtaaacg ctcagttaaa tataactgca aaaaataaca cttcttacgc
      361 tctagctgcc taaaaaccag caggcgtgac ccgatttgga ttgctcgtgt tcaatgacag
      421 gtcttattat tagcgagata cgattaagcc ttgtctagcg gtttgataag agattgatag
      481 actcgcagtt tctagacttg agttatgtgt cgaggggctg ttaaaataat acataaccta
      541 tggttgtaga caaatatgtt ggcaggtgtt tggacgtggg ttcgactccc accggctcca
      601 ttattccttt gcattctttt gcattccttg gtaaaacgtt gttaaatcaa cgttttttat
      661 ttttatcttt ggtattcctt tgcattcttt tgctaaaaag ggagtcacaa acagacccta
      721 ttttaaaaaa aggatagaaa aaaggataca acatttgtcg catcctaaaa ataatctttt
      781 ttccacggaa gacatgggat tcgaacccac gcacgctatt acacgcctac cgcgtttcca
      841 acacggcctc ttaagcctct tgagtaatct tccaatactt actcaaatag tctaccataa
      901 aggctcttat cttgcaataa aaattctaga aataagaaaa atgatagatt ttgaaagaaa
      961 atgataaaaa atgcttgact tcaaaagaaa gtatgataga atgaatagtg taaacgataa
     1021 caggaggtga ttcggtgtta aaaacagagc ggaagcaact gattttagag gagttaaatc
     1081 aacatcatgt agtttctcta gaaaaattag ttagtttgct agaaacgtca gaatcaacgg
     1141 ttcgaagaga cttggatgag ttggaagcgg aaaacaagct tcgtcgtgtg catggtggag
     1201 cagaactccc ccactcctta caggaagaag aaaccattca agaaaaatct gtcaaaaacc
     1261 ttcaagaaaa gaagttgctg gctcagaaag cagcctctct cattaaagaa aaagatgtca
     1321 tctttatcga tgctggaaca acaactgctt ttttgattca tgaattggtc aataagaata
     1381 ttacagttgt gaccaactcc attcaccatg ccgctcagtt ggttgaaaag cagattccaa
     1441 ctgtcatggt tggaggaaac gtcaagacgg cgacagatgc tagtatcggt ggcgttgctc
     1501 ttaaccagat taaccaattg cactttgacc gtgcctttat cggaatgaat ggtgttgacg
     1561 atggctatta tacgactcct gatatggagg agggagctgt gaaaagagct attttggaga
     1621 atgccaagca gacctacgtc ttggtggatt cgtcaaaaat tggacaaact tgctttgcca
     1681 aggtagcacc actcaaacgc gctatcgtta tcactagtca agggcatgag ctcttgcagg
     1741 ttattaagga gaaaacggag gtaatagaag tatgatttat acagtcacac tcaatccatc
     1801 cattgactat atcgttcgtt tggaccaagt caaagttggt agtgtaaatc gtatggacag
     1861 tgatgataag tttgctggtg ggaaaggaat caatgtcagc cgtgtcttga aacgtttgaa
     1921 tataccaaat acagcgacgg gatttatcgg tggctttact ggtaaattta tcacagatac
     1981 tttagcagag gaagaaatcg agacacgttt tgtccaggtg gcagaagata ctcgtatcaa
     2041 tgttaaaatc aaagcagacc aagaaacaga aatcaacgga acgggtccaa ctgttgaatc
     2101 ggttcagcta gaagaattga aagctatttt atctagtctg acagcagaag atacagttgt
     2161 ctttgcaggt tcaagtgcta aaaatctagg caatgttatc tataaggatt tgatttcctt
     2221 gacgcgccag actggtgcgc aagtggtctg tgactttgaa ggacagacct taattgatag
     2281 tttggactac cagcctcttc ttgtaaaacc aaacaatcat gaacttggag cgatttttgg
     2341 ggttaaactc gaaagtttag atgaaattga gaaatacgct cgtgagttac tggctaaggg
     2401 tgctcaaaat gttattatct ctatggctgg tgatggtgcc cttcttgtca catctgaggg
     2461 agcttacttc gctaaaccaa tcaaaggaac agtcaaaaat tcagttggag ctggtgattc
     2521 tatggttgct ggattcacag gtgaatttgt caaatcaaaa gacgtagtag aagccttcaa
     2581 atggggagtg gcttgcggaa cggcaactac cttctcagat gacttggcaa cggcggaatt
     2641 tattaaagaa acatatggaa aagttgaggt agaaaaacga tgaaaattca agacctattg
     2701 agaaaagatg tcatgttgct agatttgcag gcaactgaaa aaacagctgt catcgacgag
     2761 atgattaaaa atttgacaga ccacggttat gtaacagatt ttgaaacatt taaagaagga
     2821 attttggcgc gtgaagcttt gacttctact ggtttgggtg atggaatcgc aatgcctcac
     2881 agcaaaaacg ctgctgtcaa agaagcgaca gttctatttg ctaagtcaaa taagggtgtt
     2941 gactacgaga gcttggatgg acaagcaact gacctcttct tcatgattgc agctccagaa
     3001 ggtgccaatg atactcactt ggcagccttg gcagaattgt ctcaatactt gatgaaagac
     3061 ggttttgcag acaaacttcg tcaagcaaca tctgcagacc aagttatcga actttttgac
     3121 caagcttcag aaaaaactga ggaacttgtt caagcacctg ctaatgactc tggtgacttt
     3181 atcgtagctg ttacagcttg tacaacaggt attgcccaca cttacatggc ccaagaagcc
     3241 cttcaaaaag tagctgctga aatgggggtt ggtatcaagg tcgaaaccaa cggtgctagc
     3301 ggtgttggaa atcaactaac tgcagaagat atccgtaagg ctaaagctat tatcattgca
     3361 gcagacaagg ccgttgaaat ggatcgattt gatggaaaac cattgatcaa tcgtccagtt
     3421 gctgacggta tccgtaagac agaagagcta attaacttgg ctctttcagg agatactgaa
     3481 gtctaccgtg ccgctaatgg tgccaaagct gcaacagcct ctaacgaaaa acaaagcctt
     3541 ggtggtgcct tgtacaaaca cttgatgagt ggtgtatctc aaatgttacc attcgttatc
     3601 ggtggtggta tcatgattgc ccttgccttc ttgattgacg gtgctttggg tgttccaaat
     3661 gaaaaccttg gcaatcttgg ttcttaccat gagttagctt ctatgttcat gaaaattggt
     3721 ggagctgcct ttggtttgat gcttccagtc tttgcgggtt atgttgccta ctctattgct
     3781 gaaaaaccgg gtttggtagc aggtttcgtg gctggtgcta ttgccaaaga aggttttgcc
     3841 tttggtaaaa ttccttatgc cgcaggtggt gaagcaactt caactcttgc aggtgtctca
     3901 tctggtttcc taggtgccct tgttggtgga tttatcgcag gtgccttggt tcttgccatc
     3961 aagaaatacg ttaaagttcc tcgttcactc gaaggtgcta aatcaatcct tctattgcca
     4021 cttcttggaa caatcttgac aggatttgtt atgctagctg tgaatatccc aatggctgca
     4081 atcaacactg ctatgaatga cttcctaggc ggtcttggag gaggttcagc tgtccttctt
     4141 ggtatcgtcc ttggtggaat gatggctgtt gacatgggtg gaccagttaa taaagcagct
     4201 tatgtctttg gtacaggtac gcttgcagca actgtttctt caggtggttc tgtagccatg
     4261 gcagcagtta tggctggagg aatggtgcca ccacttgcaa tctttgtcgc aactcttctt
     4321 ttcaaagata aatttactaa ggaagaacgt aactctggtt tgacaaacat catcatgggc
     4381 ttgtcattta tcactgaggg agcgattcca tttggtgccg ctgacccagc tcgtgcgatt
     4441 ccaagcttca tccttggttc agcagtagca ggtggactcg ttggtcttac tggtatcaaa
     4501 ctcatggcgc cacacggagg aatcttcgtt atcgccctta cttcaaatgc tctcctttac
     4561 ctcgtttctg tcttggtagg agcaatcgta agtggtgtgg tttatggtta cctacgcaaa
     4621 ccacaagcat aaaaaataga aaaatgaaaa gattggaccg tttggtgcag tctttttctc
     4681 ttcccgaaat gcctgtgaaa tatggtataa tagaagaatg gcaaacaaga atacaagtac
     4741 aacaagacgg agaccgtcta aagcagaact ggaaagaaaa gaagcgattc aacgaatgtt
     4801 gatttcgtta ggaattgcga ttttattgat tttcgcagcc ttcaaattag gggctgcagg
     4861 tataaccctt tataatttaa ttcgcttgct agtgggtagc ctagcttatc tggcgatatt
     4921 cggcctatta atctatctct tctttttcaa gtggatacga aaacaggaag gactcttatc
     4981 tggctttttc accatatttg ctggcttact cttgattttt gaggcctact tggtttggaa
     5041 atatggtttg gacaagtccg ttctaaaagg gaccatggct caggttgtga cagatctgac
     5101 tggttttcga acgactagct ttgctggagg gggcttgatc ggggtcgctc tttatattcc
     5161 aacagccttt ctcttttcaa atatcggaac ttactttatt ggttctatct tgattttagt
     5221 gggttctctc ctagtcagcc cttggtctgt ttacgatatt gctgaatttt tcagtagagg
     5281 ctttgccaaa tggtgggaag ggcacgagcg tcgaaaagag gaacgctttg tcaaacaaga
     5341 agaaaaagct cgccaaaagg ctgagaaaga ggctagatta gaacaagaag agactgaaaa
     5401 agccttactc gatttgcctc ctgttgatat ggaaacgggt gaaattctga cagaggaagc
     5461 tgttcaaaat cttccaccta ttccagaaga aaagtgggtg gaaccagaaa tcatcctgcc
     5521 tcaagctgaa cttaaattcc ctgaacagga agatgactca gatgacgaag atgttcaggt
     5581 cgatttttca gccaaagaag cccttgaata caaacttcca agcttacaac tctttgcacc
     5641 agataaacca aaagatcagt ctaaagagaa gaaaattgtc agagaaaata tcaaaatctt
     5701 agaagcaacc tttgctagct ttggtattaa ggtaacagtt gaacgggccg aaattgggcc
     5761 atcagtgacc aagtatgaag tcaagccggc tgttggtgta agggtcaacc gcatttccaa
     5821 tctatcagat gacctcgctc tagccttggc tgccaaagat gtccggattg aagcaccaat
     5881 ccctgggaaa tccctaatcg gaattgaagt gcccaactcc gatattgcca ctgtatcttt
     5941 ccgagaacta tgggaacaat cgcaaacgaa agcagaaaat ttcttggaaa ttcctttagg
     6001 gaaggctgtt aatggaaccg caagagcttt tgacctttct aaaatgcccc acttgctagt
     6061 tgcaggttca acgggttcag ggaagtcagt agcagttaac ggcattattg ctagcattct
     6121 catgaaggcg agaccagatc aagttaaatt tatgatggtc gatcccaaga tggttgagtt
     6181 atctgtttac aatgatattc cccacctctt gattccagtc gtgaccaatc cacgcaaagc
     6241 cagcaaggct ctgcaaaagg ttgtggatga aatggaaaac cgttatgaac tctttgccaa
     6301 ggtgggagtt cggaatattg caggttttaa tgccaaggta gaagagttca attcccagtc
     6361 tgagtacaag caaattccgc taccattcat tgtcgtgatt gtggatgagt tggctgacct
     6421 catgatggtg gccagcaagg aagtggaaga tgctatcatc cgtcttgggc agaaggcgcg
     6481 tgctgcaggt atccacatga ttcttgcaac tcagcgtcca tctgttgatg tcatctctgg
     6541 tttgattaag gccaatgttc catctcgtgt agcatttgcg gtttcatcag gaacagactc
     6601 ccgtacgatt ttggatgaaa atggagcaga aaaacttctt ggtcgaggag acatgctctt
     6661 taaaccgatt gatgaaaatc atccagttcg tctccaaggc tcctttatct cggatgacga
     6721 tgttgagcgc attgtgaact tcatcaagac tcaggcagat gcagactacg atgagagttt
     6781 tgatccaggt gaggtttctg aaaatgaagg agaattttcg gatggagatg ctggtggtga
     6841 tccgcttttt gaagaagcta agtctttggt tatcgaaaca cagaaagcca gtgcgtctat
     6901 gattcagcgt cgtttatcag ttggatttaa ccgtgcgacc cgtctcatgg aagaactgga
     6961 gatagcaggt gtcatcggtc cagctgaagg taccaaacct cgaaaagtgt tacaacaata
     7021 aaaaaatagc ttctttccaa gtttggaggg aagctatttt agtggctatt gattgctttt
     7081 attttctgaa gttggcgcat tggactgttt ttcgttttca gtagcaggtt tacttgaagc
     7141 aggagtagaa gagtcctgag ttgctgtttt ctgatcttct tttttctctt ccttgacgct
     7201 agattttggt gtttcctctt gctgtgtttt ttcttgacta gtgttagtct ctttagttgg
     7261 actggtgttt tccttagggg attccttttg gatttctttg acaatggttg tcgtctggct
     7321 tgtcgtaggt tcttttttaa tatttttgtt attatccaag gcgttcataa tcgtgatact
     7381 tgcggtaatc aagccaagga tactagcgat tgtagcaacg gttttttgaa agactgtaaa
     7441 gccttttttg atgtgttctg tttttggttt tgaagttcta cgatagttag acatacattc
     7501 tctcctttga ttatgattta ttatactctg tttctttaaa aaaatcttaa aaaaagagaa
     7561 atttcccttt cttgtcgcaa gctccgtttc atgatacaat agaggaagtg attttagaaa
     7621 gcgtgagaaa catgatttac tttgataatt cagcgacgac caagccttat cctgaagccc
     7681 ttgaaaccta tatgcaggtc gcttcaaaaa ttttaggaaa cccatctagt ctccatcgtt
     7741 tgggagacca agcaacacga atcttagatg cttctcgcca acagattgca gatttaatcg
     7801 gtaagaaaag cgatgaaatc ttctttacct cgggtggaac agaaggggat aactggctta
     7861 tcaagggtgt ggcctttgaa aaagctcagt ttggcaagca catcattgtt tcagccattg
     7921 aacatccagc agtcaaagag tcagccctct ggttgaaaag tcaaggattt gaagtggatt
     7981 ttgctccagt tgataagaaa ggcttggtcg atgttgaggc gttagcaggt ttgatacggc
     8041 atgatacaat cctcgtttcc atcatggctg tgaacaatga aatcggctct atccaaccta
     8101 ttgaggctat ttcagaattc ttggcagaca agccgactat ttccttccac gttgatgcgg
     8161 ttcaggcgct tgccaaaatt ccgactgaaa agtatctgac agaacgggtg gattgcgcga
     8221 ctttctctag tcacaagttc cacggggttc gaggtgttgg ctttgtctat atcaaatctg
     8281 gcaagaagat tacacctctt cttacaggtg gtggccagga gcgagattat cgttcgacaa
     8341 ctgaaaatgt ggcagggatt gcagcgacag ccaaggccct ccgtttgtct atggaaaagc
     8401 tagatatctt taggagcaag actgggcaga tgaaggcagt gattcgccaa gctcttctga
     8461 actatccgga tatttttgtc ttttcagatg aggaaaactt tgcacctcat attctgactt
     8521 ttggaatcaa aggtgttcga ggtgaagtca tcgttcacgc ctttgaagac tatgatattt
     8581 tcatctcaac aacctcagct tgttcatcta aggcaggaaa accagccggt accttgattg
     8641 ccatgggagt ggacaaagat aaggccaagt cagctgtgcg tcttagccta gacttggaaa
     8701 atgatatgag tcaggtcgag cagtttttga ccaagttaaa attgatttac aatcaaacta
     8761 gaaaagtaag ataggagcat tcatgcagta ttcagaaatt atgattcgct acggagagtt
     8821 gtcaaccaag ggtaaaaacc gtatgcgttt catcaataaa cttcgtaata atatttcgga
     8881 cgttttgtct atctataccc aagttaaggt aacagcagat cgcgaccgtg cccacgctta
     8941 cctcaatgga gctgattaca cagcagttgc agaatctctc aaacaagttt ttggaattca
     9001 aaacttttct cctgtttata aggttgaaaa atctgtagaa gttttgaagt cttctgtcca
     9061 agagattatg cgggacatct acaaggaagg tatgaccttt aagatttcta gcaagcgtag
     9121 cgaccacaac tttgaacttg atagtcgtga actcaaccaa acacttggag gggctgtatt
     9181 cgaagccatt ccaaatgtgc aagttcaaat gaaaagtcct gacatcaatc ttcaggtgga
     9241 gattcgtgaa gaagcagcct atctttctta tgaaaccatt cgtggggctg gtggtttgcc
     9301 agttggaact tcaggtaaag ggatgctcat gttgtcagga gggattgact cacctgtagc
     9361 aggttatctt gctcttaagc gtggggtgga tatcgaggca gttcactttg ctagtccacc
     9421 atatactagt cctggtgccc tcaagaaagc gcaggacttg acccgtaaat tgaccaagtt
     9481 tggcggaaat atccagttta tagaggtgcc tttcacagag attcaagagg aaatcaaagc
     9541 caaagcgcca gaagcttatt tgatgactct aactcgtcgc tttatgatgc ggattactga
     9601 ccgtattcgt gaggtacgaa atggtttggt tatcatcaat ggggaaagtc taggtcaagt
     9661 agccagccaa acccttgaaa gtatgaaggc tatcaatgct gttaccaaca ctcccatcat
     9721 tcgtcctgtg gttaccatgg acaagttgga aatcattgac atcgcccagg aaatcgatac
     9781 ctttgacatt tcaatccaac cgtttgaaga ctgttgtacc atttttgcac cagatcgtcc
     9841 aaaaacaaat cctaaaatta agaatgcgga gcagtacgaa gcgcgtatgg atgttgaagg
     9901 cttggttgag cgagcagtgg ctggaatcat gattactgaa atcacacctc aagccgaaaa
     9961 agatgaagtt gatgacttga ttgacaatct gctctaattc agaaaatcca aaagaatagc
    10021 gaaaatcagt aaaaaaagtt agttttttct ctaaaaacag gtaaaaaact aacttttttt
    10081 atttttatga tataatgata taaaattttg aatatagaga gttttctgac aatgaatcaa
    10141 tcctactttt atctaaaaat gaaagaacac aaactcaagg ttccttatac aggtaaggag
    10201 cgccgtgtac gtattcttct tcctaaagat tatgagaaag atacagaccg ttcctatcct
    10261 gttgtatact ttcatgacgg gcaaaatgtt tttaatagca aagagtcttt cattggacat
    10321 tcatggaaga ttatcccagc tatcaaacga aatccggata tcagtcgcat gattgtcgtt
    10381 gctattgaca atgatggtat ggggcggatg aatgagtatg cggcttggaa gttccaagaa
    10441 tctcctatcc cagggcagca gtttggtggt aagggtgtgg agtatgctga gtttgtcatg
    10501 gaggtggtca agccttttat cgatgagacc tatcgtacaa aagcagactg ccagcatacg
    10561 gctatgattg gttcctcact aggaggcaat attacccagt ttatcggttt ggaataccaa
    10621 gaccaaattg gttgcttggg cgttttttca tctgcaaact ggctccacca agaagccttt
    10681 aaccgctatt tcgagtgcca gaaactatcg cctgaccagc gcatcttcat ctatgtagga
    10741 acagaagaag cagatgatac agacaagacc ttgatggatg gcaatatcaa acaagcctat
    10801 atcgactcgt cgctttgcta ttaccatgat ttgatagcag ggggagtaca tctggataat
    10861 cttgtgctaa aagttcagtc tggtgccatc catagtgaaa tcccttggtc agaaaatcta
    10921 ccagattgtc tgagattttt tgcagaaaaa tggtaagtta agaaaggaaa aaacgaaatg
    10981 catattgaac atcttagcca ctggagtggt catcttaacc gtgaaatgta ccttaaccgt
    11041 tatggacatg gtgggattcc agttgtggtc tttgcttcat caggtggtag tcacaacgaa
    11101 tactatgatt ttggcatgat tgatgcctgt gcttccttta tcgaggaagg ccttgtccag
    11161 ttctttaccc tatctagttt ggatagtgag agctggttgg ctacttggaa aaatgctcat
    11221 gaccaagcgg aaatgcaccg tgcctacgaa cgttatgtga ttgaggaggc cattctttta
    11281 tcaagcacaa gacaggttgg tttgatggca tgatgacgac aggttgctct atgggagctt
    11341 atcatgcact caatttcttc ctccagcatc cagatgtctt taccaaagtg attgctctca
    11401 gtggtgttta cgacgcacgt ttctttgtcg gtgattacta caacgatgat gctatttacc
    11461 aaaactcgcc agtagattat atttggaacc aaaacgacgg ctggtttatt gaccgttacc
    11521 gtcaggcaga gattgtgctg tgtacggggc ttggagcctg ggaacaagat ggtttgccat
    11581 ccttttacaa gctcaaagaa gcctttgaca agaaacaaat tccagcctgg tttgctgaat
    11641 ggggacatga tgtcgcccat gactgggaat ggtggcgtaa acaaatgcct tatttcctcg
    11701 gtaatctcta tttataaaag gagttaccta tgaattacct tgttatttct ccctactatc
    11761 cacaaaactt tcaacagttt accatcgaac tagctaataa aggcatcaca gtcttgggaa
    11821 ttggtcaaga gtcttacgag caattggatg agcccttgcg caatagcttg accgagtatt
    11881 ttcgtgttga taatcttgag aacatagatg aagtcaaacg tgcagttgct tttctctttt
    11941 ataaacatgg tccaattggc cgcatcgagt ctcacaatga atactggctt gagctagacg
    12001 caacactcag agaacaattc aatgtttttg gtgccaaacc agaggatctc aaaaagacga
    12061 aatataagtc tgaaatgaag aaacttttca aaaaagcagg tgttcctgtg gtacctggag
    12121 ctgttatcaa gacggaagca gatgttgatc aagcagtgaa agaaatcggt cttccaatga
    12181 ttgccaaacc tgataatgga gtgggagcag ccgcaacctt taaacttgag acagaagacg
    12241 atatcaatca cttcaagcaa gaatgggacc attcaaccct ttatttcttt gaaaaatttg
    12301 tcacttccag cgaaatctgt acctttgacg ggctcgtgga caaggatgga aagattgtct
    12361 tctcaacaac ctttgactac gcctatacac cgcttgacct catgatttat aagatggaca
    12421 attcttatta tgtgctcaag gatatggatc ctaaactgcg caagtatggg gaagcaattg
    12481 tcaaagaatt tggtatgaaa gaacggtttt tccatattga gttcttccgt gagggggacg
    12541 attatattac catcgagtac aataaccgcc ctgcaggtgg ttttaccatt gatgtttata
    12601 actttgctca ttccttggac ctttatcgtg gctatgcagc tattgtcgca ggagaggagt
    12661 tcccggcgtc agactttgaa actcagtatt gtttggctac ttctcgccgt gcaaatgctc
    12721 actatgttta ttcagaagag gatttgcttg ccaaatatag ccagcagttc aaggttaaaa
    12781 aagtcatgcc agctgccttc gcggaacttc aaggagatta cctgtatatg ctgaccactc
    12841 cgagtcgaca agaaatggag cagatgattg cagatttcgg acaacgtcaa gaataagaac
    12901 tatcggatta aggaaattaa ctcccttaat ccttttgttt tgtctgataa aaaataagag
    12961 catcccaaca aggtagctat cataaaactt gttcgataac tatttgaagc aggattaggt
    13021 ggtcagaaat taaattttaa tatttcaatt gagtcatagt attgtgtttg cgtatcctta
    13081 aatcagctaa aaggatccat gacgacacct atacgatata gttttcaaga taccaaacaa
    13141 gtctattaat attcaatgaa aatcaaagag caaactagga agctagccgc aggtt
//
LOCUS       DAVBCG010000045        12965 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBCG010000045 DAVBCG010000000
VERSION     DAVBCG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.045"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            205..1887
                     /locus_tag="VQ495_001086"
     CDS             205..1887
                     /locus_tag="VQ495_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531055.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1872..2702
                     /locus_tag="VQ495_001087"
     CDS             1872..2702
                     /locus_tag="VQ495_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2531056.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2895..3398
                     /locus_tag="VQ495_001088"
     CDS             2895..3398
                     /locus_tag="VQ495_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2531057.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3452..3686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3851..4414
                     /locus_tag="VQ495_001089"
     CDS             3851..4414
                     /locus_tag="VQ495_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2531058.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4428..5054
                     /locus_tag="VQ495_001090"
     CDS             4428..5054
                     /locus_tag="VQ495_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2531059.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5166..6038
                     /locus_tag="VQ495_001091"
     CDS             5166..6038
                     /locus_tag="VQ495_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531060.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGSISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6168..6305
                     /locus_tag="VQ495_001092"
     CDS             6168..6305
                     /locus_tag="VQ495_001092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531061.1"
                     /translation="MKRSQVVKRCILIALLLLFLLNVLSGSLSVTTRGVDPRPAYGVS
                     L"
     gene            6729..7643
                     /locus_tag="VQ495_001093"
     CDS             6729..7643
                     /locus_tag="VQ495_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2531062.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7636..8649
                     /locus_tag="VQ495_001094"
     CDS             7636..8649
                     /locus_tag="VQ495_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531063.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8633..10225
                     /locus_tag="VQ495_001095"
                     /pseudo
     CDS             8633..10225
                     /locus_tag="VQ495_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            10417..11320
                     /locus_tag="VQ495_001096"
                     /pseudo
     CDS             10417..11320
                     /locus_tag="VQ495_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11338..11475
                     /locus_tag="VQ495_001097"
                     /pseudo
     CDS             11338..11475
                     /locus_tag="VQ495_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11503..11844
                     /locus_tag="VQ495_001098"
     CDS             11503..11844
                     /locus_tag="VQ495_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531064.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11817..12128
                     /locus_tag="VQ495_001099"
                     /pseudo
     CDS             11817..12128
                     /locus_tag="VQ495_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12256..12843
                     /locus_tag="VQ495_001100"
     CDS             12256..12843
                     /locus_tag="VQ495_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2531065.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
BASE COUNT         4038 a         1950 c         2689 g         4288 t
ORIGIN      
        1 actgacgtgg tttgaagaga ttttcgaaga gtataaaatg atggggaagg ctgggcaact
       61 agtctttttc gatgtttata gattagttag gcaagtaagc taggatagga atctttttgg
      121 tgctaagatt ttaagagaag agactgcaag aagggcttga tttatgataa aatagaagtc
      181 taagaagcga atgaagagag taagatgaaa gaagctataa ttgagtggaa ggatttctct
      241 ttccggtatg aaacacaaca agaaccgacc ttgcaaggga tagacttgac catttacaag
      301 ggagagaaag tcttaattgt tggaccatct ggatcaggta aatctacctt gggtcagtgt
      361 ttgaatggga ttattcccaa tatttacaag ggtcagacat atggagaatt tttgataaag
      421 ggtcaaacag cctttgatat gagcatctat gataagtctc atctggttag cacagttttg
      481 caggatacag atgggcagtt tattggcttg tctgtggcag aagatttggc gtttgctctg
      541 gaaaatgatg tgacagccct agatgagatg aaaggtcgtg tttataaatg ggctgaaaag
      601 ctggaccttc ttcctttact ggatcagcgt cctcaggatt tgtcaggtgg acaaaagcag
      661 cgagtcagtc tggctggtgt cttgattgat gaaagtccga ttctcttgtt tgatgagcca
      721 ctcgccaatc tagatcccaa gtcaggtcag gatattatcg aattgattga ccagattcat
      781 aaggaagagg ggacgacgac tcttattatc gagcaccgtt tggaggatgt tctgcatcgc
      841 cctgtggatc ggattgtctt gataaacgat ggtcgtatcc tttttaatgg gagccctgac
      901 cagttgcttg cgactgattt attgactcaa aatggaattc gagaacccct ttatctaacc
      961 actctccgtc aattaggtgt ggacttagtc aaggaagaac agttagcgaa tctggataac
     1021 atgtctatct caaaaggtca ggttcagctg cagaatgaac tggtaaaaga aactccagaa
     1081 ttgcagtcac tctttaaatt agaggacgtg tctttctctt atgatgatag accgatttta
     1141 aaatccctac atttagatat taaaaagggt gaaaagattg ctattgtcgg aaaaaatgga
     1201 gcagggaaat caactctagc caaggctata agtagcttta ttcagacgga aggacgctat
     1261 ctttgggaaa aacaggatat aaaaggcgat tctgttgcag agcgggcgga acgagtaggc
     1321 tatgtgctac aaaatcctaa tcaaatgatt tcaaccaata tgatttttga tgaggtggct
     1381 ctagggctcc gtttgcgagg tgtggatgag aaggaaattg aaacgagagt ctatgaaacc
     1441 ttgaaaatct gtggacttta tgaattccgt aattggccta tttctgccct gtcatttggt
     1501 cagaaaaaac gtgtcaccat tgcttcaatt ttggtcttag gagctgaaat tattctccta
     1561 gatgaaccga ctgcaggtca agatcagaag aactatactg agattatgga atttctcgaa
     1621 gagttacatc gaaaagggca taccattgtc atgattaccc atgatatgca attgatgctg
     1681 gattattcag accgagtcct tgtcatggtg gatggagaat tgattgccga tactgttcca
     1741 gccagtctgt tgagcgatcc tgagctgtta gtaaaagcca atctaaaaga aacctccatc
     1801 tttaacttgg ctaagaaact agatgtggat ccactggatt taacggcatt ttacaaagaa
     1861 aggagagagg gatgcaagct aaattaatcg gttaccagca tagagatact gtgattcatc
     1921 gcttgtcagg agctgggaaa cttctctttt tcattctggt atcattggcg gccatgatta
     1981 gctatgatac cagaatgctc ttgctgattg ccatcttttc agtctttctc ctctatttat
     2041 cagaaattcg ctttaaagat gtttcctttg tagccgtttt tgcgacggta tttgccgttt
     2101 taaacgtttt gatggtctat ctcttttctc ccgagtatgg ggttggactt tatggagaga
     2161 gaagtgtgat ttggcaggga atcggtgtct acactctaac cagccaggag ctcttttatc
     2221 tgctaaattt ggccattaag tatctttgca ccattcctct ggctattatc tttttgatga
     2281 caacccatcc tagtcagttt gcttccagtt taaatcaaat tggtgtgccc tataagattg
     2341 cttattctgt cagcctgacc ttgcgctata ttccagattt gcaggaagaa ttctttacta
     2401 tcaagatgtc tcaggaggcg cgtgggatgg aattatccaa gaaagcttct cttatgcaac
     2461 gaatcaaagg caatctgctc attattacgc ccttgatttt tagctcgcta gaacgcattg
     2521 ataccattgc gactgccatg gagcttcgcc gctttgggaa agaaaaaaaa cgcacatggt
     2581 atagttatca ggccttgaaa aaaggagact atattacctt gctcttggca accttgtttt
     2641 tagtagctag tttactactt agcttgcaga atcagggacg attttacaac ccttggaaat
     2701 agcttgaaaa aattgaaaaa atcaagtcgt ttctattgac aatgattctg aaagtgttat
     2761 actaagaaag tagtttcgct gatttacttc aaacctgttg tgaggtaagt taacgatgcc
     2821 ttaaccacgc tgtttgctga gcttgactcc gggcagtgtg gctatttttt tgcaatggtg
     2881 aaaggaagca agtcatgaca aatcacattg tattatttga acctcaaatt ccacaaaata
     2941 cgggcaatat cgcgcgtact tgcgctgcga ccaattctcc cctccacatc atcaagccta
     3001 tgggctttcc tattgatgac cgcaagatga agcgggctgg attggattac tgggataagc
     3061 tagagattta tttttacgag agtttggaag atttcatatc tcagatgaag ggaaaactct
     3121 atctgatttc gaaattcgcg gaaaaagtct attctgaggt ggatttatcg actgacgagg
     3181 accattattt tctctttgga cgtgaagaca agggcttacc tgaagatttt atgcgagaac
     3241 atcctgagaa ggctctccgt attcccatga atgatgaaca tgtccgcagt ctcaatgtgt
     3301 ctaataccgt ctgcatgatt gtctatgagg ctcttcgcca gcagaacttt gcaggtcttg
     3361 aactcgtcca tacctatgaa gtggataaat tgaaataaaa aatgaaaatg aacaaaatgc
     3421 ttgcgcttgc aagcgttttt tgttatgata aaagagtctt cagggcaggg tgtgattccc
     3481 gaccggcggt gactttaact aggaaatgat cttttccttt tatactttgt tgacaagctt
     3541 tgcctaacca gaagttatgc ctacagcttg tcgcctagtc taaaagaaaa atctctattt
     3601 ccttctcttt aaagaagtcc gcgagcgcaa gctgatgtgg tgagattcca caaccgacag
     3661 tatagtctgg atgggagaag acgaaagaat agctttgtct gttttgatag atttatagcc
     3721 ggattgtaac cacttgcttt gtttttcttt aatagagtag gagggaactt ttgggatata
     3781 aaaagtgaga atagatagag gaatcctttc caacttcttc tgattttata gaaaattgga
     3841 ggaacctgtt atgacaaaca cacgtcgact ttcgaccatt gcaattttat cagccatctc
     3901 atttgtgctg atgtactttg actttccgct tttaccagcg gcatctttcc tcaagatcga
     3961 atttagtatc ttgccagtcc ttgtgggtct ggtggtcatg gatttgcctg ctgctctagg
     4021 agtgctcttg cttcgctcac tcttgaaatt gcttcttaac agccaaggag tgaatactta
     4081 cattggtttg ccgatgaata tcgtagcttt gggagttttt gtcatcgtat ttgctttgat
     4141 ttggaaaaag gaacggacaa cccttcgttt cctactaggc tctctagctg ggactattgg
     4201 tttaaccgtg gctatgttag ttctcaacta tgtttatgct gttcctttgt acgctaagtt
     4261 tgctaacttt gatattggaa aaattttggg actttccaac tacctaatga ccatggtatt
     4321 accttttaac ttgattgaag gtgtaatctt ttccgtttca ttctggttgt tgtacattct
     4381 cttgaaacca accttaaaac attatgaaag ataaacaaac atttttaatg aagggcagtt
     4441 ttgccctttt acttttcgtt attcttggct atatggtcaa attttaccct gaaatgttgg
     4501 tcaattttga ccaatcgatt cagactgcca ttcgaggaga cttaccagat tatttgacta
     4561 ttctttttcg atccctcaca cgtctgattg atattccagt gattatcact tgggttgtca
     4621 ttatagcttt tgtcttttat cgtaagcgat ggaagataga aagtttcttc atgctgggaa
     4681 atctgacttt ggcaggtctt ttaatcgtga cctttaaaaa tatctaccag cgcccacgac
     4741 cagctatctt acatctggtg gaggagaagg gattttcctt cccaagtggg cattctctgg
     4801 ctgtaacgct gatggtaggg actttgattg ttattcttag tcagcggatt aaagatcagg
     4861 tctggagaaa aatcgtacaa atcgtccttg gcctctacct agtcagtgtg ctggtatcaa
     4921 gagtctatct gggagttcat tacccatcag atgttcttgc cagtctctgt gtgggcttgg
     4981 gagccctgtt tatcgagttt cccttttatg ataagctccg cttccaatgg cgatttaaag
     5041 gcaaacagaa atgatggaaa agataaaaaa ttgtacaaat acaaatagaa ccatatgaaa
     5101 tatatacttg aaacatgttt tggagtttga tatattttaa taaataaagc aaggaggaag
     5161 aaagtgtggg tatgttatta gcgacacgct taaaaaataa aagaaaggaa ttaaaattat
     5221 ctcaaaaaga attagctgag gggatttgtg agcaagggca gattagccgt atggagcaag
     5281 gtaaatactc acctgggtca gaattactct ttcaactatc taagcgattg aaggtgagca
     5341 tggattattt ctttgaagat actgaggttt ctagtttaga aaatattgat aagtttaaag
     5401 aattatctaa gaaatttttg gatgagaggg aatatgaatc tctccattac atctatgaac
     5461 tggaaaaatc taaacgtgct cgtctatctg ctaaagacca gatttatttg gattggattg
     5521 atacattagt tcaattctgt tatttaaatc atcaaacagg ggcagttgcg aaactggaaa
     5581 aaatactgtt atctctgaaa aaatctgatt tttactatct acaactggga aataccttgg
     5641 ctaattttta ttttgatatg gggcaggaag acacatatca ggaactctat caacttttaa
     5701 aagagaacct ccagtcacta tctgttcatc atttggaaca gttagaattg atgattaaat
     5761 tccgatataa ctattgtaga tatttgtggt tgaatgaacg aacatctgaa gctatagaag
     5821 aaattacaga aacgatagct atttgtaaaa gatataatag tagttatcga ttagcagatt
     5881 tgtattgttt attgggaagt ataagcgagg gctttgctga aagggatagg atactaggtt
     5941 tttatcaagt atcttaccag ctttacaagc acgaagagaa ccagagaatg atgctagaaa
     6001 ttgagaagga aatgaaatta ttggaaagtg aagattaagc aaaaagattt gtattcttag
     6061 ataactttaa tacatattat aaaaaaaata aaaaaatatc ataaaaatta ttgacaaacc
     6121 aaaattgatg tgttagaata tcaccaatag ttaaggaggt ttagaagatg aaaagatctc
     6181 aagttgtcaa acgatgtata ttaattgctt tattgcttct atttctactt aatgtgttaa
     6241 gtggttcttt atctgtaaca actagaggag ttgatccaag accagcatat ggagtttcgc
     6301 tttaaagttg tgcatagttc ttcttttata gacggagagt ttttctcaat agtaagtgat
     6361 tcaaattttg atggatgatt ttccattaaa ttaagtgatt gcgaaaactt tttgaaatac
     6421 attctctttg gatattgaaa gtttgttttg gattttttta tgaagcttat cttagctgtt
     6481 agattggggt tttatgggat ttgtcctcac cgggaaaaaa tcttaaatct ttgaagaaag
     6541 aggtttttat cttatggtta gagatacatc agaagaaatt gaagttattg acttgtcttc
     6601 tatgaccgtt tcctactcat ctgttatgct aggaaactct tggatgacgt ggaattgatt
     6661 tattaagata gattatttgg tttttaccaa aaatctgtct ttttaatttt taaaggacta
     6721 atggtgatat gaaactatat caaataaatg atgggataca aatttttgaa agtagtaatc
     6781 ttgaaaaaat agtaattaga aattatagga atggtaagaa aataactatc gatcaccaac
     6841 cattattaaa aaaattacta ctattccttg aagttcctaa acgtgaagaa gaaattctat
     6901 tatatcttcc agaattatca aataaaacat tacgaagtgt tttgacgaat tttatgagtt
     6961 taaatataat tcattgtcaa gatacattaa taattcacaa aaaaataaac atattgataa
     7021 taggtttagg gactacgggt ggatatctgg cagatgggtt atgtcgtagt aatattaatt
     7081 cgaagctatt tcttttagat aaggatcgtg tagatgagac taatgtaggt agacagattt
     7141 atttaagaga tgatattggt caatataaag ttgatgtatt taaacaaaag tttagtgata
     7201 aagaaataaa ggtattcaaa atttttatta atagtaagaa agatttggaa aagatttgca
     7261 aaagggaggg tatcgatttg gttatacaat gtggtgactc cccttcacct cgagaattag
     7321 gaaaaattgt gaatacagta tgtgatactc tccagatacc ttatatagtt aacactggct
     7381 acatgagtaa tgtagttcct ttgccagaat tttattatcc aaataataga tacaatttta
     7441 attataagca tcaattttca gatgaaaagt tactgttctc acaagttttg tctaaagctg
     7501 attatatggt ggcaattcaa tcatcatttg taatgttaat gcaaataaaa aattttatat
     7561 ttaatgaaga acctatttat tatggttata ggggatacta taattctgta acattagcat
     7621 gggaggtgga aaaaattgac taaatattta gaattggatt attctcattc atatattttg
     7681 gaaggtttta gaaaaaatga tgcgtttatt gaaaatcaca aagaatgcct tgatatgtta
     7741 tcagataaga tttggcgaga taataaatat atgaatacag aaaaaaacat atcatcaccg
     7801 acacgaacaa tcttatcacg ctatacttgt aatattttat cgtctttgtt tattgatatt
     7861 agcaaaaata gcatagaaaa attgatagtg acatgtgaaa atagcgatct taattggatt
     7921 tataattatt ataaatcggt ttacaatgga gtattttgtg aattgcgaca acttatacaa
     7981 cagtactgta aggtgaagga taagtatgaa aagtggcttt ttataaaagc tattataaat
     8041 caaggtattc gtcaaaatga gaaagaagtt gttgaattct ttttaaaaca attgaaggat
     8101 aataatcaag gtatttttga ttacatcaat agttttagtt tttatctatt aaagttttat
     8161 tcaaaagaca aatctcgcat ttttttagaa gagcagctag atatagatga ttttttggga
     8221 tcagataaag aagattatgc taggtcttta gtttttaaga attatgcttc tctattgcct
     8281 gatacttctg aattaaaaag aaatattatg gaaaaatgtt taagtcaaac acctcaagat
     8341 acagatttat ggaaagaatg gtttgaaact tatgccagtc agaaagagat tagacaaaaa
     8401 gcaacggaaa tatttaagca tggttattca gatatttcgt tactaaaatt agttaaaatt
     8461 gagcctgatg acactgagag tttaatcaga atgattatct tatgtaccag tgacctaaat
     8521 agtaatattg caaggtattt gatttcattt ttgagcgatg ggcgattaaa agaatttttg
     8581 gagttatttg ttgaaaattt tgactttttg aatattattg agggaaaaac tagtgagatt
     8641 aatttttaga ataattagaa aagatattat attatattct atattaacat ttttgctatc
     8701 aatagcgatg gtaagcgaag catttttgat gcaatttatt gttgattcaa ttaaatttgg
     8761 tgaaaagaag tatgttttga tttctatttt tattgtaatt tttttgattc ttcagacttt
     8821 attgtattac tatcaacaat ttcttacagc tgtattaagt aaaaaatctg catatgagta
     8881 ccgtaaatta atttttaaaa atattcagca gacttctctt aggcttgtta caggtgaaaa
     8941 aaatgatagt attctagctt ctttgacaac gcaaatcgat caaatagaat ataattattt
     9001 ttattctgtt tattggggtg gatatttgct ttgccaatta gtggtagcta tagttatgtc
     9061 gctttatcta aatccattga tgtctgtatt aaccatttgc ttaagtctcc ctaatttatt
     9121 tgtagcactt ctttttaaaa actccttgga acaaaagcaa gaagagctaa ttaatgagac
     9181 aaattcctca atagcagtta ctcaagattt aattgaggga attactgatt ggaaaattgc
     9241 aaatagagaa cctaatatat ttagagtgtt tgaaagcaaa acattgaagc ttttagagaa
     9301 gcaagtgaag gtagaaaaat ttcaatacat tgttgttagt ttaaatcaat tgttttcaaa
     9361 tgttttatat tttggtagtt ggatagtaag tggattcttg attatacggg ggcaactttc
     9421 attagggagt ttgatagcgt tctctcaatt attagcaaga atttcttacc ctgtttacgc
     9481 ttcttaggat ttattgtcaa aatatattag cggtaaaaaa attgtagaaa ttctttctaa
     9541 agaatttata ttttcagacg aaagcagaaa ttatccagat gagtttaatg ttatttcatt
     9601 tgaacattat tttacaaaca ttgacgaaaa ggatatgcct tttgataaag aattttacaa
     9661 aaataaaaaa tatttgttga aagggaaaag tggtagtggg aaaacaacaa ttttaaaatc
     9721 gattttacga gagtatgatg attatagtgg aattgtaaaa attaatgggg tagatgttag
     9781 ggatataaaa gagagtgttc tttttgacaa tatagcttat gtttcccaaa aacctcactt
     9841 gttcagcgca agtcttagag acaacctgac attattttct gaagacttca ctgataatga
     9901 gatagttgag gttttaaaat ttgttgaatt atctaaatgg gctaataaag attgcttgga
     9961 tatgatgctt tcaaatgatg atataaaatt atcaggaggg gaggtcaaaa gggtttcttt
    10021 agctcgagcc ttattgatga ataagaatat tctgttattg gatgagttct ctgctgggat
    10081 tgattatgaa acattgttaa aaatagagaa gaaactcatt gatttggata agatgattat
    10141 ttacgtgacg catgtggata tagatagatc cggcaaacag tttgatgatg tcattgattt
    10201 ggattgtctt tttatatcct cgtgacatga gagttgagtc agttgaatta ttttgtcact
    10261 ctgtttagtt cttgttggta gtgtcaggta atctttgtta atttttcagg aagtctattg
    10321 aaatatcctc ttggttatat attacgagga gtaattaatg gatattctcg agaattcaat
    10381 acaacaattt gattctaaag ttaagtatgt ttctttattt gcggaatcaa tcttttatca
    10441 gataccatga gctattttgc aggtgctatg atttctcctt tgtatgtgag gattatcgga
    10501 gatgatatga cagatgctat gggggttaga caggcaactg ctggtgtagt taatattctc
    10561 agcaacctta tagggggagt tttgattggg ggtcatcagt cttcagatgt ttgtaggttt
    10621 gaatgctttg acctttcttt ttgcactgtt gggtgtggtg tttattcgta cttctttgag
    10681 agatattgag gaacagatgg aggttagtaa aagattagcc gcaaaatctt ttggagagca
    10741 tttcctccat tcaatgaaga tattgtcaag ttttccagcc atcatcaaat taatattcat
    10801 tgcagctatg aaccaagtga ttttaagcat tattatccct ttaacgactt tgatgttaat
    10861 ccatcatcca tttatcttct ttgacactgg gaaatccatt gcagcattgt cagtggttat
    10921 tttttcagga acgattgtgg gaagtttttt gagtggaggt atcctaaaaa atattcccat
    10981 aaaaatgatt gtctactttg aagaaattat gcatctctgt attctctttt cttggttgac
    11041 caatcatttc ctccttttat tggtagttgt cttcttatgc gcagttgcag taggaatgat
    11101 ggggcctcgg attttcaacc ttgtattctc gatggttcca gaagaagtca tgggaaccat
    11161 ccagtcagtg cttggagtat tcaatgttgt tgtaccagga attcttagca tgctcatcgt
    11221 tgccttagcg agtgctacaa acctaaaaat cacagtgctt ccacttttta tttttgtatt
    11281 gactgcctta gcaattgtga aagcaatgaa aatagagtaa tagtagtatg aactactgtc
    11341 tctttttggg tgggagttca ttacccatca gacgtccttg ccagtctctg tgtgggcttg
    11401 ggagttttgt ttatcgagtt tcccttctat gacaagctcc gcttccaatg gcgatttaaa
    11461 ggcaaacaga agtgagtatc gaattccctt gaggagaaag aaatgaaagt caatatagca
    11521 gatcttcatc cgactcaact atatttatca gaaaagaagt tgcaagatat tcagatgctt
    11581 taccagtcgg cagaaacaat ccaagtcgat ccaatcagta ttcttgcctt tggagattgt
    11641 ttgctgatta cagatgggca tcacagggct tatcaggctt tattggcagg tcgggatacg
    11701 atttctgctg agtgggatag agatggtggt gatgaactat atcatctcta tgcgcaagct
    11761 tgcgaggaaa gaaagattta ctctgttttt gatttaaaaa atcatatctt acctctttgg
    11821 atcggcatgg tgagcttttt ttgattgaag tcaaggagaa gggagaatgg tttccaattg
    11881 gggatattac actatctcag gataatctcc ccattgtgat tgggaattcc gcttaccaac
    11941 atcgaggact tggaaaaaag attctaagtg ctttgattga attggctcga gtaaaaggat
    12001 ggaaagaatt gagagtcaag gaaatctaca cctacaatca tgcttctagg aggtgtttca
    12061 agtcgcttgg atttgtggaa aatggagcaa cagaaaaagg aaggagtttt atattggaat
    12121 tagtctaatc ctgccacttt ctccccctta acctttgact attccgcaaa attatcgtaa
    12181 aataaagagt aaatgataaa atgaggtcag agtctgttcg ctctgacgat agtagtataa
    12241 atgaggagaa acgctttgga attagaagta tttgctgggc aagaaaaaag tgaactatct
    12301 atgattgagg tagcgcgtgc tatattagaa cttcgtggtc gcgatcatga gatgcatttt
    12361 agcgatcttg taaacgaaat tcaaaactac cttggaacat caaacagcga tatccgcgaa
    12421 gctttgcctc tgttctacac agagttgaac tttgacggta gcttcatctc acttggggac
    12481 aacaaatggg gtcttcgttc atggtatggt gtggacgaaa tcgacgaaga aatcatcgct
    12541 cttgaagaaa atgacgacga tgaagtagca ccaaaagcta agaaaaaacg tgtcaatgcc
    12601 tttatggatg gtgattcaga tgccattgac tacaatgcag atgatccaga agacgaagat
    12661 gcatacgaag cagatccagc tctttcatac gatgatgaaa atccagatga tgaaaaaaat
    12721 gaagtggaag cttatgatgc agaaatcaac gaaatcgctc cagatgactt gggagaagat
    12781 gtggatctca acgaagacga cgacgagttt tcagatgacg acgctgaaac cagcgaggaa
    12841 taagagttag ctattgacaa ttatcctatt tttaggtatc atattgtttg ggcacctctt
    12901 ttagaggtcg gggctcccta gttcttaggg agctattttt gttttttcaa gaagttatct
    12961 tcttg
//
LOCUS       DAVBCG010000046        12551 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBCG010000046 DAVBCG010000000
VERSION     DAVBCG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.046"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            92..346
                     /locus_tag="VQ495_001101"
     CDS             92..346
                     /locus_tag="VQ495_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET2531066.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            350..604
                     /locus_tag="VQ495_001102"
                     /pseudo
     CDS             350..604
                     /locus_tag="VQ495_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            795..2408
                     /locus_tag="VQ495_001103"
     CDS             795..2408
                     /locus_tag="VQ495_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2531067.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            2538..3164
                     /gene="gmk"
                     /locus_tag="VQ495_001104"
     CDS             2538..3164
                     /gene="gmk"
                     /locus_tag="VQ495_001104"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2531068.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            3189..3503
                     /locus_tag="VQ495_001105"
     CDS             3189..3503
                     /locus_tag="VQ495_001105"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2531069.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            3569..5965
                     /locus_tag="VQ495_001106"
     CDS             3569..5965
                     /locus_tag="VQ495_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2531070.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGTVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            5978..6913
                     /locus_tag="VQ495_001107"
     CDS             5978..6913
                     /locus_tag="VQ495_001107"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2531071.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            6906..8219
                     /gene="rsmB"
                     /locus_tag="VQ495_001108"
     CDS             6906..8219
                     /gene="rsmB"
                     /locus_tag="VQ495_001108"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2531072.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            8234..8974
                     /locus_tag="VQ495_001109"
     CDS             8234..8974
                     /locus_tag="VQ495_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2531073.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVFMNEEDAE"
     gene            8971..10950
                     /gene="pknB"
                     /locus_tag="VQ495_001110"
     CDS             8971..10950
                     /gene="pknB"
                     /locus_tag="VQ495_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2531074.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            11065..11823
                     /locus_tag="VQ495_001111"
     CDS             11065..11823
                     /locus_tag="VQ495_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2531075.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            11825..12274
                     /locus_tag="VQ495_001112"
     CDS             11825..12274
                     /locus_tag="VQ495_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2531076.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            12336..12551
                     /locus_tag="VQ495_001113"
                     /pseudo
     CDS             12336..12551
                     /locus_tag="VQ495_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         3738 a         2487 c         2991 g         3335 t
ORIGIN      
        1 aatagataga aaagaaaatc ttgtacaagt tcctgacaat ttctttcttt tcgtgtataa
       61 tagtggaaaa gagctaaaac gaggtatggt tatggaagca gtcctttact caacattccg
      121 aaatcattta aaggactaca tgaagaaggt aaatgatgaa tttgagcctt tgacggtggt
      181 caataaaaat ccagatgagg acattgtagt tctttcaaag agcgaatggg acagtattca
      241 ggaaaccctg agaatcgccc aaaataagga gttatcagac aaggttttgc gaggaatggc
      301 tcaagtccgt gctggaagca ctcaggtcca tgtgattgag gagtgatgaa tgctactcaa
      361 gtttacagaa gatgcctggg cagattattg ctactgacaa aaccaagata aaaaaacact
      421 aaaaagaatc aataaattaa tcaaggatat tcaacgcgat ccatttacag gaataggaaa
      481 accagagcca ctcaaatatg attaccaagg agcctggtca cggcgtattg atgcagaaaa
      541 tcgcttgatt tatatgatgg atggagatag cgtggctttc ttgtccttta aagatcatta
      601 ctaagtctac tgcaaagtag gctttttctc aagtctctac taaactacta tttctaaaca
      661 acaagtttat tttggtaaat gtgttccaaa ttccccaaaa catattctat tttaggtttg
      721 tgaaaatcac ttttttatgg tagaatgtaa aagaatgtat gtcattcgga ataacaataa
      781 aatgaggtaa aaacatggaa atcatgtcgc ttgcgattgc tgtttttgcc gtcatcattg
      841 gtttagtcat tggatatgtc agcatctcag ctaagatgaa atcatctcag gaagctgcag
      901 agttgatgct tttaaatgct gaacaagaag caactaattt acgtggacaa gctgagcgtg
      961 aagcggattt acttgttaat gaagccaaac gtgaaagcaa gtctcttaaa aaagaagcac
     1021 tattggaggc caaagaagaa gccagaaaat accgtgaaga agtggacgct gaattcaaat
     1081 cagaacgtca agaactcaaa caaatcgaaa gtcgtttgac agagagagct actagccttg
     1141 accgtaagga cgacaatttg acgagtaaag aacaaacact tgaacaaaaa gaacaaagta
     1201 tttctgatag agcgaaaaac cttgatgcgc gtgaagagca attagaggaa gtcgaaagac
     1261 aaaaagaagc agaactagag cgtattggtg ctctgtctca ggcagaagca cgagatatta
     1321 tcttggctca gacagaggaa aacttgacca gggagattgc cagtcgcatt cgcgaagctg
     1381 agcaagaggt caaggaacgt tctgacaaaa tggccaagga catcttggtt caagctatgc
     1441 aacgtatcgc tggtgaatat gtagcggagt caacaaactc aacagttcat ctgccagacg
     1501 atactatgaa gggacgcatt attggtcgtg aaggtcgtaa cattcgtacc tttgaaagtt
     1561 tgacaggggt cgatgtgatt atcgacgata caccagaagt ggtgaccttg tcaggatttg
     1621 atccgattcg tcgtgagatt gcccgtatga ctatggaaat gttgctcaaa gatggtcgta
     1681 tacatccagc tcgtatcgaa gagttggttg agaaaaaccg tcaagagatt gacaataaga
     1741 ttcgtgaata cggtgaggct gctgcctatg aaattggtgc gccaaacctt catccagact
     1801 tgatgaagat tatgggacgt ttgcagttcc gtacttcata tggacaaaat gttttgcgcc
     1861 attcgattga ggttgctaag ttggctggta tcatggcgag cgaacttggt gaaaatgcgg
     1921 ctcttgcccg tcgtgctgga ttccttcacg atatcgggaa agccattgac catgaggttg
     1981 aaggtagcca cgttgaaatc ggtatggaat tggcccgtaa gtacaaggaa cccccagttg
     2041 tggtgaatac gattgctagt caccacggag atgttgaagc tgagagcgtg atagcagtta
     2101 tcgtcgctgc agcagatgcc ttgagcgcag cccgtccagg tgctcgtagt gagtctcttg
     2161 aaagctacat caagcgtctc catgatttgg aagaaattgc taacggcttt gaaggagtgc
     2221 aaactagctt tgcccttcaa gcaggacgtg aaattcgtat catggtcaat ccaggaaaaa
     2281 tcaaggacga caaagtcaca atcttggctc acaaagttcg taagaaaatt gaaaacaatc
     2341 tcgattatcc aggaaatatc aaggtaaccg tgattcgcga gcttcgtgca gtagattatg
     2401 ctaaataaat aagaaagagc agttgaaaaa ttactgcttt tttgttacac tagatagaaa
     2461 gactgtaaag gataatctgg ctttttgcca ttattctaga gaaatgttat ttcacagatt
     2521 gactaaaagg agacacaatg gcagaccgag gcttactaat cgttttttct ggtccttcag
     2581 gggttggaaa aggaacggtt agaagagaga tttttgagag ttctgaaaac caatttcaat
     2641 attctgtatc gatgacgaca cgcgcacaac gtcctggaga agtggacggt gttgactatt
     2701 tcttccgtac tcgtgaagaa tttgaagagc tgattcgtca aggacagatg ttggaatacg
     2761 cagaatatgt cggcaactac tatggaactc ctctgaccta tgtcaatgaa accttggaca
     2821 agggaatcga tgttttcctt gaaattgaag ttcagggtgc tcttcaggtc aagaaaaagg
     2881 ttccagatgc tgtctttatc ttcctgacac caccagattt ggatgaattg caagatcgct
     2941 tggtaggtcg tggaacagat agtgcagaag tgattgccca acgaatcgaa aaggccaagg
     3001 aagaaattgc cctcatgcgt gagtatgatt atgcgattgt caacgatcag gtgcccctag
     3061 ctgctgaacg tgtcaaatgt gtgattgaag cagaacactt ctgtgtggat cgtgtcattg
     3121 gtcactatca ggagatgtta ccaaaatctc caactacccg ataaaattta gaaaataggt
     3181 acaagcaaat gatgttaaaa ccctctattg ataccttgct cgacaaggtt ccttcaaaat
     3241 attcactcgt aatcttggaa gcaaaacgtg cccacgaatt ggaagcaggt gccccagcaa
     3301 ctcaaggttt caagtctgaa aaatcaactc ttcgcgcttt agaagaaatc gaatcaggaa
     3361 acgttacaat tcacccagat ccagaaggaa aacgtgaagc agtgcgtcgc cgtatcgaag
     3421 aagaaaaacg ccgcaaagaa gaagaagaaa agaaaatcaa agagcaaatt gctaaagaaa
     3481 aagaagatgg tgaaaaaatt taaggttggg gggactcaat cttatttttt ctattgcaag
     3541 aatgtactga caaggaggag gtgagaagat ggccctagct aagattatcg tagatgtgcc
     3601 cttgatgcag acggaccagc cctatagtta caggattccg gaggaatttg agggaatgct
     3661 ggaagttggg atgcgggttc atgtgccttt tggtaagggt aatcgcctga ttcaagggat
     3721 tgttcttggt ttgaagtccc aatcagatgg agaagagatg gagcaagatt taaaagatat
     3781 tgcagaggtg ctggattttt ctcctgttct cacgccagaa caactctggc tggctgagga
     3841 gttacgcaaa tctgtcttct cctacaaaat ctctatcctc aaggccatgc ttccagggtt
     3901 tctgaattct agctatgaca agattctcta tcctctggaa ggcttgagtc aggaagaacg
     3961 agtacgcctg tttggttcag aagattcgct agccttttct tcactagacc ttgccaagca
     4021 agctgaaatg atgcgtttga ctagaaaagg cctgcttggt ctggaatatc aggcagtcga
     4081 tcaaaagaag gtcaagaccc agtcttggta tgaggttgac catgcccaat tagaaggtgt
     4141 tgagatttct acacgtgcca agaaaaagtt ggaactgaga gattatctgc tgtctcatcc
     4201 agagagcgct tccttggcta gcttgttaga gtcctactcg cgagagcaag tcaacttctt
     4261 tgtggatcaa ggtactgtta ccatagtcca aaaggaagtt caacgctcgg ctgcttattt
     4321 tgaaggaatt gaagcaagta gacctttgga gttaaatcca gaacaaagac aggcgcgtga
     4381 tgcggttgtg agttctattg gcagttctca acctcccttt ctacttcaag ggattacagg
     4441 aagtgggaag accgaggttt acttgcagat tatccaaggt gccctggata agggtaagac
     4501 agctattttg ctggtacctg agatttcctt gactcctcag atgaccgagc gttttatagc
     4561 acgttttggg gacaaggtag ccattcttca ctcaggatta tccaatggcg aaaagtatga
     4621 tgaatggcgc aaggtggagc gtggcgatgc ccaagttgtt gttggtgcca gatctgctat
     4681 ctttgctcct ctgaaaaatc tgggtgtcat gattattgat gaagagcatg aagcggctta
     4741 taagcaggac agcaatcccc gttaccatgc cagagaggta gctattttac gggctcagta
     4801 taatcaagca actctggtac ttggatctgc aacgccgagc ttagagagcc gggcacgtgc
     4861 tggcaaaggc gtctatcaac acttacgtct aacccaacgt gccaatcctt tggctacaat
     4921 ccctgaggtt caagtgattg actttcgaga ctatatcgga caaaatgaga cgtcaaactt
     4981 tacgcctcct ttgctagagg ctatccaaga ccgtctggtt aaaaaagagc aggtggttct
     5041 catgctcaat cgccgtggtt attctagctt tgtcatgtgt cgggagtgtg ggacggtgga
     5101 tacttgtccc aactgcgata tttctctgac cttgcatatg gataccaaga ctatgaactg
     5161 ccattattgt ggtttctcga aggatattcc tcaggtctgt cctaactgta agagccgcag
     5221 tattcgttac tatgggacgg gaactcagaa ggcttatgat gagctagcag aactctttcc
     5281 ccaagctcgc attttgagga tggatgtgga tacgactcga aagaaaggca gtcaccaagc
     5341 cttgcttgac cagtttgggc gaggggaagc ggatatttta cttggtactc agatgattgc
     5401 aaagggtttg gattttccca atgttaccct agtcggagtt ctaaatgcgg atacggcctt
     5461 gaatctgcct gatttccgtt cttctgagag aaccttccag ctcttgactc aggtggcagg
     5521 tcgagcaggt cgggctgaaa aagcaggtca agtcttgata cagtcctaca attcgcagca
     5581 ctatgctatt cgatttgcca aggatcaaga ttacgaaggc ttttatgcct atgaaatggg
     5641 aatcagacga caactcggct atccacctta ctatttcacg attggtatta ccctttctca
     5701 caagaaagaa gaagaggttt tcaaacgtgc ctatgaagtc atgaacattt tgcggtcagg
     5761 cttgtcagag accagtccca ttctggggcc aacgccaaaa ccgattgccc gtacccacaa
     5821 cctctatcat taccagattt taattaaata ccgtttagaa gatgagctgg ggccgaccct
     5881 caaccaggtc ttggccttga ctcaagaacg ggaaaatagt gagctccgtc tcagcattga
     5941 ccatgagccg cagcaatttt tataagaagg agaagatatg acaaaactaa tctttatggg
     6001 gacccccgac ttttcagcaa cagtcttaaa aggacttttg acagatgacc gttacgaaat
     6061 tctagccgtt gtgacccagc cagaccgtgc tgttggtcgt aaaaaagtta tccaagaaac
     6121 cccagtcaag caggctgcca aggaagcagg actatctatc taccaacctg aaaaattatc
     6181 tggaagtcca gagatggaag aacttatgaa gctaggagca gatggaattg tgactgctgc
     6241 ttttgggcag tttctcccaa gcaaactcct tgatagcatg gactttgctg tcaacgttca
     6301 tgcctccctc cttcctagac accgtggtgg tgcgcctatc cattatgcct tgattcaagg
     6361 ggatgaggaa gctggtgtga ccatcatgga aatggttaag gaaatggatg caggagatat
     6421 gatttctcgt cgcagcattc cgatcacaga tgaggacaat gttggcacct tgtttgaaaa
     6481 attggcgcta gttggtcgtg atttgctttt ggacactctg cctgcctata ttgctggtga
     6541 tatcaaacct gaaccgcagg atacgagtca ggttaccttc tctccaaata taaagccaga
     6601 ggaagaaaaa ctggactgga acaaaaccaa tcgtcaactc tttaaccaaa ttcgtggaat
     6661 gaacccctgg cctgttgccc atactttcct taagggcgac cgctttaaga tttatgaagc
     6721 cctaccagta gaaggtcagg gaaatccagg tgagattctc tctatcggca agaaagaatt
     6781 gattgtcgca acggctgaag gggctctatc cctcaaacaa gtgcagccag ctggtaagcc
     6841 taagatggac attgcttcct tcctcaatgg agttggacgt acattgactg taggagaacg
     6901 atttggtgac taaagtagaa acggctagaa gtttagctct agcagtgcta gaggatgttt
     6961 ttgtgaatca agcatattca aatattgcct taaataaaca cctcaagggg agtcagctct
     7021 ctgcagcaga caagggctta gtgaccgagc tagtctatgg aacggtagcc cgtaaactga
     7081 ctctggaatg gtacctatcc cactttatcg aagacagaga ccagttagac agctggctct
     7141 atgtccttct tctcatgagt gcctaccaac tccgctattt ggacaagatt ccagatcatg
     7201 ctgtggtcaa tgaagcagtg gaattggcca aactccgtaa aaaaggcagt gaaaaattgg
     7261 tcaacgctgt ccttcgccgt atcttgcgtg aaggctggcc agatattgct agcatcaagc
     7321 gaaaaaacaa gcgtgactcc attgcctatt ctctcccagt ttggctagtt gccaaactca
     7381 aggaagaata cggagaggag agagcaaaag ccatctttga aagcctgtta gtgcgaaaca
     7441 aagccagtat tcgtgtaaca gacctaagtc gaaaagagga aatccaagcc ttgttggagg
     7501 cgaataattc ccttttagca accactggtc tggttaagga gcaggggcat tttgcagggc
     7561 ataatttgtt tgcggatgga gccattacca tccaagacga gtccagtcag ttggttgctc
     7621 cgacgcttga tttacaaggt gatgagcgag tgcttgatgc ctgtgcggct ccaggtggga
     7681 aaacagccca tatagcctct tatcttacga caggtcaggt tactgctctg gacttgtacg
     7741 accacaagtt ggatttaatt caagaaaatg cccaacgtct gggagttgaa gatcgggttc
     7801 aaactcaaaa attggatgcc agaaaggtgc atgagttttt tgaccaggat tcctttgata
     7861 agattttggt ggatgctcct tgttcaggaa tcggtctttt gcgccgaaaa ccagatatca
     7921 aatacaataa agaaacggca gatttcgcgt ccttgcagga aattcagtta gaaatattag
     7981 gtagtgtttg tcaaatacta ggcaaaggtg gtataataac ttatagcacc tgcactatcg
     8041 tctcagagga gaattttcaa gtcgttaagg cctttttaga gagtcatccc gagttcgagc
     8101 aggtaaaact agaacatgaa tgtaaggata tcatgaaaga cggctgtatc ctcattacac
     8161 ctgaattgta tggaagtgat ggattcttca tcagtcaatt tcgcaagata tcggattagg
     8221 aaggaactga cacatggaaa tttcattatt aacagatgtt ggtcagaaac gaacaaataa
     8281 ccaagactat gtcaaccact atgtcaatag agctggacgt accatgatta ttttagctga
     8341 tgggatggga ggtcatcgtg cagggaatat cgctagtgaa atggcggtca cagacctggg
     8401 tgtagcttgg gttgataccc agatcgatac agtcaatgaa gtgcgtgaat ggttcgccca
     8461 ttacctagaa attgaaaatc aaaagattca ccagcttggt caggatgaag cttacagagg
     8521 catgggaact actttggaag tccttgctat tattgataat caggctatct atgctcatat
     8581 tggtgattcg cgtatcggct tgattcgtgg agaagaatac catcagttga cgagcgatca
     8641 ttccttggtt aatgaattgc tcaaggctgg tcaattgaca ccagaagagg cagaagctca
     8701 tccgcaaaaa aatattatca cccagtctat tgggcaaaaa gatgaaattc agcctgattt
     8761 tgggacagtt atccttgagt caggtgacta tctcttgctc aatagtgacg gcttgaccaa
     8821 catgatttca ggcagtgaga ttcgtgatat tgtaaccagt gatattcctt tagcagataa
     8881 aacggagaca cttgttcgtt ttgctaacaa tgcaggaggt ttagacaaca ttacggttgc
     8941 ccttgttttt atgaacgagg aggatgcaga atgatccaaa tcggcaagat ttttgccgga
     9001 cgctatcgga ttgtcaaaca gattggtcga ggaggcatgg cggatgtcta cctagccaaa
     9061 gacttaatct tagatgggga agaagtggca gtgaaggttc tgaggaccaa ctaccagacg
     9121 gacccgatag ctgtagctcg ttttcagcgt gaagcgagag ctatggcaga tctagaccat
     9181 cctcatatcg ttcggataac agatattggc gaggaagacg gtcaacagta cctagctatg
     9241 gagtatgtgg ctggactgga cctcaaacgc tatatcaagg aacattatcc tctttctaat
     9301 gaagaagcag tccgtatcat gggacaaatt ctcttggcta tgcgcttggc ccatactcga
     9361 ggaattgttc acagggactt gaaacctcaa aatatccttt tgacaccaga tgggactgcc
     9421 aaggtcacag actttgggat tgctgtagcc tttgcagaga caagtctgac ccagactaac
     9481 tcgatgttgg gctcagttca ttacttgtca ccagagcagg cgcgtggttc gaaggcgact
     9541 gtgcagagtg atatctatgc catggggatt attttctatg agatgttgac aggccatatc
     9601 ccttatgacg gggatagcgc ggtgaccatt gccctccagc atttccagaa acccctgccg
     9661 tccgttattg cagaaaatcc atctgtacct caggctttag aaaatgttat tatcaaggca
     9721 actgctaaaa agttgaccaa tcgctaccgc tcggtttcag agatgtatgt ggacttgtct
     9781 agtagcttgt cctacaatcg tagaaatgaa agtaagttaa tctttgatga aacgagcaag
     9841 gcagatacca agaccttgcc gaaggtttct cagagtacct tgacatctat tcctaaggtt
     9901 caagcgcaga cagaacacaa atcaatcaaa aacccaagcc aggctgtgac agaggaaact
     9961 taccaaccac aagcaccgaa aaaacataga tttaagatgc gttacctgat tttgttggcc
    10021 agccttgtat tggtggcagc ttctcttatt tggatactat ccagaactcc tgcaaccatt
    10081 gccattccag atgtggcagg tcagacagtt gcagaggcca aggcaacgct caaaaaagcc
    10141 aattttgaga ttggtgagga gaagacagag gctagtgaaa aggtggaaga agggcggatt
    10201 atccgtacag atcctggcgc tggaactggt cgaaaagaag gaacgaaaat caatctggtt
    10261 gtctcatcag gcaaacaatc cttccaaatt agtaattatg tcggccggaa atcttctgat
    10321 gttatcgcgg aattaaaaga gaaaaaagtt ccagataatt tgattaaaat tgaggaagaa
    10381 gagtcgaatg agagtgaggc tggaacggtc ctgaagcaaa gtctaccaga aggtacgacc
    10441 tatgacttga gcaaggcaac tcaaattgtt ttgacagtag ctaaaaaagc tacgacgatt
    10501 caattaggga actatattgg acggaactct acagaagtaa tctcagaact caagcagaag
    10561 aaggttcctg agaatttgat taagatagag gaagaagagt ccagcgaaag cgaaccagga
    10621 acgattatga aacaaagtcc aggtgccgga acgacttatg atgtgagtaa acctactcaa
    10681 attgtcttga cagtagctaa aaaagttaca agtgttgcca tgccgagtta cattggttcc
    10741 agcttggagt ttactaagaa caatttgatt caaattgttg ggattaagga agctaatata
    10801 gaagttgtag aagtgacgac agcgcctgca ggtagtgtag aaggcatggt tgttgaacaa
    10861 agtcctagag caggtgaaaa ggtagacctc aataagacta gagtcaagat ttcaatctac
    10921 aaacctaaaa caacttcagc tactccttaa aagcagatgg ataatcaaaa tgagtaatca
    10981 atctttgtca tgatttcatg gcaaagattt tttttgagtc cggatttgtg atagaataga
    11041 gggagttgat aaaaggagga aagcatggaa gaatcaaaag aattaaatgc cgtcattgat
    11101 gtgattatgc tagcggggac tattctcctt aaaagtggct cagaaatcca tcgtgtagaa
    11161 gataccatga ttcgaatcgc gcattcgcag gggattgtgg attgcaatgt ccttgccatg
    11221 cctgccgcta tctttttctc tattgaaaat accaatattt cgcgcatgaa gcgcgtgacc
    11281 tcctcttctt ataacatcga aaaagtctgc gatgtgaacc agatttctcg tcagctagtt
    11341 ggggggcaga ttgacttaga aacagccttt aagcaattga cggccttgca agctcaaccc
    11401 cttccctata ctaagttgca ggtaactctg gctgcgacct ttagtgctcc tttcttttca
    11461 gttatgttta gcggaaatat ctacgacgca cttggggcag gagtggcgac cttatttggt
    11521 tttgcctttt ccctctatgt ggaaaaattt atccgaattc cctttgtgac agcctttgct
    11581 ggagcctttg tctttgggat aattgcccag ttttgggctc gctacacagg ttttccttca
    11641 acggcagatt tgattatagc tggtgcggtc atgccgtttg taccaggtat tgccttgacc
    11701 aatgcggtcc gtgatattat gaccaaccac ataaactctg gtatgagtaa gatgtttgaa
    11761 tccctgctca ttaccctcgc tttaggggca ggaacttctg tcgccttggt attgatgaac
    11821 taatatgaca ctaacaacct ttttattaca agcagtagca agttttcttg ccattatcac
    11881 ttttttaatc gtactcaatg tgcaacggtc tatgctctta cctggaggga ttttgggcat
    11941 gactgtctgg ctaatctatc tcttgctcaa ggaaccgacc aatgtcattg tagctacctt
    12001 cattgcagcc attattggtt cttgtgtcag ccagatttta agtattcttt ataagacacc
    12061 tgctgtggtc tttatcttgg ccattttggc accgctggtt ccaggttatc tctcctaccg
    12121 aacaactgcc ttttttgtga caggggacta taataaagca ctggcaagtg cgaccttggt
    12181 tgtcatgttg gctttggtaa tctctattgg aatggctagc ggaacagtga ttctcagact
    12241 gtatcattat ataaaaacac atcgagtatc gtagacttta cagaaataaa agaattttct
    12301 gaaaaatgag ataaataaat taacaacgct ttctatatgt gcgagaatac cgcacttatg
    12361 aagaaattgc ggctgatttt ggtatccacg aaagcaactt actccgtcgg agccaatggg
    12421 ttgaagtaac tcttgttcaa aggtggtgtt acgatttcaa gaactcctct cagttctgaa
    12481 gacacggtaa tgattgatgc gacggaagta aaaatcaatc gccctaaaaa aaacaattag
    12541 cgaatgattc t
//
LOCUS       DAVBCG010000047        12469 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBCG010000047 DAVBCG010000000
VERSION     DAVBCG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.047"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ495_001114"
     CDS             complement(251..433)
                     /locus_tag="VQ495_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531077.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ495_001115"
     CDS             complement(649..1881)
                     /locus_tag="VQ495_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2531078.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ495_001116"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ495_001116"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2531079.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ495_001117"
     CDS             complement(3048..4217)
                     /locus_tag="VQ495_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2531080.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ495_001118"
     CDS             complement(4403..5707)
                     /locus_tag="VQ495_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2531081.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ495_001119"
     CDS             complement(5824..6978)
                     /locus_tag="VQ495_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2531082.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ495_001120"
     CDS             complement(6975..7526)
                     /locus_tag="VQ495_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2531083.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ495_001121"
     CDS             complement(7519..8655)
                     /locus_tag="VQ495_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2531084.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ495_001122"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ495_001122"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2531085.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ495_001123"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ495_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2531086.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ495_001124"
     CDS             complement(10172..10903)
                     /locus_tag="VQ495_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2531087.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ495_001125"
     CDS             complement(10905..12350)
                     /locus_tag="VQ495_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2531088.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4192 a         2422 c         1640 g         4215 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcgtccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactacat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaa
//
LOCUS       DAVBCG010000048        12287 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBCG010000048 DAVBCG010000000
VERSION     DAVBCG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.048"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            81..878
                     /locus_tag="VQ495_001126"
     CDS             81..878
                     /locus_tag="VQ495_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2531089.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1026..1148
                     /locus_tag="VQ495_001127"
     CDS             1026..1148
                     /locus_tag="VQ495_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2531090.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            1170..2210
                     /locus_tag="VQ495_001128"
     CDS             1170..2210
                     /locus_tag="VQ495_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2531091.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2381..3655
                     /locus_tag="VQ495_001129"
     CDS             2381..3655
                     /locus_tag="VQ495_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2531092.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3665..4543
                     /gene="truB"
                     /locus_tag="VQ495_001130"
     CDS             3665..4543
                     /gene="truB"
                     /locus_tag="VQ495_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2531093.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4811..5086
                     /locus_tag="VQ495_001131"
     CDS             4811..5086
                     /locus_tag="VQ495_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2531094.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5194..5832
                     /gene="udk"
                     /locus_tag="VQ495_001132"
     CDS             5194..5832
                     /gene="udk"
                     /locus_tag="VQ495_001132"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2531095.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5960..7300
                     /locus_tag="VQ495_001133"
     CDS             5960..7300
                     /locus_tag="VQ495_001133"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2531096.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7278..7490
                     /locus_tag="VQ495_001134"
     CDS             7278..7490
                     /locus_tag="VQ495_001134"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2531097.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7487..8362
                     /locus_tag="VQ495_001135"
     CDS             7487..8362
                     /locus_tag="VQ495_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2531098.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8355..9170
                     /locus_tag="VQ495_001136"
     CDS             8355..9170
                     /locus_tag="VQ495_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2531099.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9163..9594
                     /locus_tag="VQ495_001137"
     CDS             9163..9594
                     /locus_tag="VQ495_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2531100.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9601..11268
                     /gene="recN"
                     /locus_tag="VQ495_001138"
     CDS             9601..11268
                     /gene="recN"
                     /locus_tag="VQ495_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2531101.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11270..11998
                     /locus_tag="VQ495_001139"
     CDS             11270..11998
                     /locus_tag="VQ495_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2531102.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12077..>12287
                     /locus_tag="VQ495_001140"
     CDS             12077..>12287
                     /locus_tag="VQ495_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2531103.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIEL"
BASE COUNT         3680 a         2209 c         2940 g         3458 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atggtttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat atagaaaact
      961 gatatttgta aactataact caaggtgcta caatatcctt aataaaatga tatggaggtc
     1021 accttatgac ttgtgatttt aaatttgaaa ctctacaact acatgctggt caagttgtgg
     1081 ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatctttt atttttgatg
     1141 acacgtagga aggtgctgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta
     1201 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1261 gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg
     1321 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1381 gtatcacaac aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag
     1441 acaataccaa gcttgtcttg attgaaacct tgggtaactc cttgattaat attccagacc
     1501 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1561 ttgcaatacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc attcactctg
     1621 cgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1681 gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt ccaagctgcc
     1741 acaatttgag ttatacttgt gatgtgggtg cagcagcctt tattatagct gttcgagttc
     1801 aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa
     1861 gacttgaaac ctcttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1921 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1981 cttatcatgc cttggctgag aaatacttgc caaaagatgt cggttcaatc tttaccttcc
     2041 atgtcaaagg tggcgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2101 accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg
     2161 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaactaa attcgtttgt
     2221 caatcggtct tgaaaatgta gaagatttga ttgaagactt gcgcttggcc ttggaaaaaa
     2281 tttaaagtaa aagaagataa acagtgggct tcgactcact gtttttgatt ttccctcagg
     2341 catgatataa tggttaaaga agtctagaaa gaggaacgat atgaacgaaa tcaaatgtcc
     2401 caactgtggg gaagtcttta cagtaaatga gagtcagtat gccgaactct tgtcccaagt
     2461 gagaacggca gagtttgata aggaactaca cgataggatg aagcaggaac tggccttggc
     2521 tgagcaaaag gccatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2581 gcaattacag agtcagatcc aaaactttga tacagaaaaa gaattggcca agaaagaggt
     2641 tgaacagaca agccatgagg ctctcttggc taaggacaag gaagtacagc tcttagaaaa
     2701 tcagttggct accttgcgtt tggagcatga aaatcaactg caaaagaccc tttctgacct
     2761 agaaaaagaa cgggatcagg ttaaaaacca actacttttg caggaaaagg aaaatgaatt
     2821 atctttggct tctgttaagc aaaactacga agcccagctc aaggcagcta gtgaacaagt
     2881 cgagttttat aaggatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2941 acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt
     3001 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3061 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcagacgg
     3121 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3181 gaagaactgt gagtatgccg ttttggtgac catgcttgag gccgataatg actactttaa
     3241 cacagggatt gttgacgtca gccacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3301 ctttatccag ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3361 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagacttgga
     3421 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3481 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgactac
     3541 atctgaaaac caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3601 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg agtagaaagc
     3661 aaaaatgaac ggtattatca acttaaaaaa agaagcagga atgacctcgc atgatgcggt
     3721 ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa ccttggatcc
     3781 ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg tcgagtttat
     3841 gcaggacgag ggtaagatct atgaggggga aatcattctg ggctattcca cgacgactga
     3901 ggatgctagt ggggaagtgg tcgcagaaac ccctgttttg tctcccttgg atgaaaagct
     3961 tgttgatgaa gcgattgcta gcttgactgg gcctattact cagattcccc ctatgtattc
     4021 ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc aggaagtgga
     4081 gcgtccagaa cgtcaggtga ccatttatca atttgagcga acaagtccga tttcttatga
     4141 tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggactt acatccgtac
     4201 tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc atttgactcg
     4261 tactagtgct gctggcttac aattagaaga cgctcttacc ttggaggaaa ttgctgaaaa
     4321 agtagaggct gggcaattag attttctcca tcctttagag attgggacag gtgaccttgt
     4381 caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt ttattgagct
     4441 agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag ccattctaga
     4501 aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt aggaataaaa
     4561 atcgggtgat agataacaat tgcttgataa ccccccatac taatagtaga atggttttgg
     4621 gaattataat attccaattg ttgcgagttg tagatactca aataatctat atagaaattt
     4681 agaggtgtga aatgaagcaa cttaaaattc tttcagataa atatttagag tccatgacag
     4741 gttctggtgg gaacttaggt ctaggatttg gtgtgataat tccatgatgt gaaatgagtt
     4801 tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata gtgagttgag
     4861 tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc aatcggtcat
     4921 ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc aggcacctaa
     4981 gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa gagggagact
     5041 ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact gtcgaacatc
     5101 agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa aaatgtgata
     5161 gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat cattattgga
     5221 gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt atcgcatttt
     5281 cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca gtctcatttg
     5341 acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga tacagacttg
     5401 atgatcgagc agattaagga attgttggca gggcgtccgg tggacatccc gacctacgac
     5461 tatacagagc atacacggag tagcaagacc tatcgtcagg agcctcaaga tgtctttatc
     5521 gttgagggca ttttggtctt ggaggacaag cgtctgcgcg atttgatgga tatcaagatt
     5581 tttgtggata cggatgacga tgtgcgtatt attcgtcgta tcaagcgtga tatggaggaa
     5641 cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa accaatgtac
     5701 caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga aggggttagc
     5761 aataccgtgg ctatcgacct gttgacgacc aagattgcaa agattttgga agaagctcga
     5821 aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc ctcataatgg
     5881 tacataaatg gagattttta ggcatatttt tggtataata atacccataa aagagcaagt
     5941 aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga ccaaatatct
     6001 gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg gtcaagtttc
     6061 caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc atgcagttat
     6121 tcaagcgacc atctggtctg ggatttatca gaaattaggg tttgacctgg aagaaggaat
     6181 gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct actccatcat
     6241 cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg aacaacttaa
     6301 gaaaaaattg acagaagaag gcctgtttca agaacgcttc aagcaagctc taccccaatt
     6361 ttctaagaga attggggtag taaccagccg tagtggagcc gttattcgag atattatcac
     6421 gaccgtcagc aggcgattcc caggtgttga catccttctt tatcctacca aggttcaagg
     6481 tgaaggggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac gggacgattt
     6541 ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg cctttaacga
     6601 agaaattgtg gtacgagcta tttttgaatc tcgtttgcca gttatctcta gtgtggggca
     6661 tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa cgccaacagc
     6721 ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt tgcaaaatca
     6781 ggaaaaacgg atggcaacgg cagtccgaaa tgttctatct aagaaacaag aggctttgaa
     6841 aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg gttatttgca
     6901 acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga tttctgataa
     6961 caaacaatta gttcaagcaa gaactcatca attagtacaa ttatcacctg ttaccaaaat
     7021 ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc aaatggcgtt
     7081 agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc tcatgttgga
     7141 tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt ccgttgtaga
     7201 ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag atggtcaagt
     7261 agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta gcagaactgg
     7321 aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg attactgcct
     7381 ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag gctgaaaaga
     7441 ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga aaaagcaaga
     7501 aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc agtttgcctc
     7561 tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta ttcggccttt
     7621 tctcttgtta gaagttctgg aatccttgca ggttaccatc aaacctgctc acgcgcaggt
     7681 agctactgcc ttggagatga ttcatacagg gagcttgatt cacgatgacc ttcctgctat
     7741 ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg gtgaagctat
     7801 ggccattttg gctggagatg ccttattctt agacccatat gccttgattg cgcaggcaga
     7861 tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt caggtagtct
     7921 gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact tgtctctgga
     7981 agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc ccttccaagc
     8041 ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa ctgtgggtga
     8101 attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag ctagttttga
     8161 ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct atcctgcctt
     8221 gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag ctaatgaaaa
     8281 attagaagaa attgcccagc agcttccctt tgaaacagaa tcgattgtaa gtgtagtaga
     8341 aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa caggggttgt
     8401 ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg tctagtcgta gcagtcctta
     8461 atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa ttaaaactca
     8521 agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag gccttgcagg
     8581 tctttgattt gtcggtggat ggcgcgacta cgattgatat cggggcctct actggaggtt
     8641 ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat gttggtacca
     8701 atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag cagttcaatt
     8761 ttcgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt attgatgtga
     8821 gtttcatttc ccttagtctg attttgccag ccttgcacag tgtcttggct gatcaaggtc
     8881 aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt gggaaaaatg
     8941 gaattattcg agatgctaag attcatcaga atgtccttga atctgtaaca gctatggcag
     9001 tagaggcagg tttttcagtc cttggcttgg acttttctcc catccaaggt ggacatggaa
     9061 atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag attcttgctg
     9121 agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa aaaagaaaga
     9181 cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga agaaattgta
     9241 gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg agatatcaaa
     9301 gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga attgccaaaa
     9361 tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga attgatggat
     9421 aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc tcagttaatc
     9481 gagacttttg cagacaagat ttttagctgt ttgactgatg atagctcgat tttagtcatt
     9541 gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg gtaggtctat
     9601 atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc cctcaatttt
     9661 gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat tatcattgat
     9721 gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca tggtgcacca
     9781 aaggcagaga ttgaggggct tttctcagtt gagaatagtc gtcttttaca ggaaattttt
     9841 gatgagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat cttgcaaaat
     9901 ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt gcgaactatt
     9961 gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat gcgtccccaa
    10021 ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt gaaagaaacc
    10081 tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga agtcaagaaa
    10141 aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc agagattgag
    10201 gcagcaaact tgcaggctgg agaagacttg gatctcaatc aagagcgaga taaactcctc
    10261 aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga caatgaagat
    10321 ttttcaagtc tggccaatgt tcgttcagcc atgaatgaca tggaaagtgt cgaagagtat
    10381 gaccctgaat accgtgaaat ttcaagctct ctgtctgaga cctactatgt tttagaagac
    10441 attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa tcgcctcatg
    10501 caggttgaga atcgtttgga cctccttcat actattactc gtaagtatgg tgggactgtt
    10561 gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt gacaggcaat
    10621 aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa tcttgtcaat
    10681 ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga agctgagatt
    10741 aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttcg ttttagtaag
    10801 ggaaaattca gtcgtgaggg aaataaaatg gttgagtttt atatttcaac caaccctgga
    10861 gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg tctcatgtta
    10921 gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt tgatgaggtg
    10981 gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca taaaattggt
    11041 cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat tgcagattat
    11101 caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt tcgtctcttg
    11161 acggtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga tgtgacagaa
    11221 gcagccctga cgcaagcaag agaattgttg agaaacaggg agaaataaga tgacagatta
    11281 ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc tcaaaacctg
    11341 ggatggtcag ccccagttgc tctttctagg tgatttgatt gacagaggtg aggatagtca
    11401 ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct gtttgtccgg
    11461 aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt atgaccatta
    11521 tagtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct tggatgcacc
    11581 agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcggcagact tggtcgaatt
    11641 cattcgtcaa atgccatttg tagtagagac agacaagtat atctttgttc acgcaggtat
    11701 tgatttgacc ttggacgact ggcatgaaac aacagattat aaaaaagtat ggcttagaaa
    11761 accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc atacaccggt
    11821 ctatggtttg ttacagcaag aacgcggtac atctgagctt tggactacag aagatggcaa
    11881 gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg tctttacaga
    11941 ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg aagattagta
    12001 ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa atagataccc
    12061 tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacaggaga agatccgtaa
    12121 cttctctatt atcgcccata ttgaccacgg aaaatcaact ctagcagacc gcattttgga
    12181 aaaaacagag acggtttcaa gtcgtgaaat gcaggcccag cttttggata gcatggagct
    12241 agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttga
//
LOCUS       DAVBCG010000049        12218 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBCG010000049 DAVBCG010000000
VERSION     DAVBCG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.049"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(225..2483)
                     /locus_tag="VQ495_001141"
     CDS             complement(225..2483)
                     /locus_tag="VQ495_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2531104.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2712..2942)
                     /locus_tag="VQ495_001142"
     CDS             complement(2712..2942)
                     /locus_tag="VQ495_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2531105.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3132..3815
                     /locus_tag="VQ495_001143"
     CDS             3132..3815
                     /locus_tag="VQ495_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2531106.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3815..4549
                     /locus_tag="VQ495_001144"
     CDS             3815..4549
                     /locus_tag="VQ495_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2531107.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4703..5560
                     /locus_tag="VQ495_001145"
     CDS             4703..5560
                     /locus_tag="VQ495_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2531108.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5702..6574
                     /locus_tag="VQ495_001146"
     CDS             5702..6574
                     /locus_tag="VQ495_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2531109.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLMTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6856..7539
                     /gene="rpiA"
                     /locus_tag="VQ495_001147"
     CDS             6856..7539
                     /gene="rpiA"
                     /locus_tag="VQ495_001147"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2531110.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7553..8764
                     /locus_tag="VQ495_001148"
     CDS             7553..8764
                     /locus_tag="VQ495_001148"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2531111.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIRESNKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8766..9308
                     /locus_tag="VQ495_001149"
     CDS             8766..9308
                     /locus_tag="VQ495_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2531112.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9324..10133
                     /locus_tag="VQ495_001150"
     CDS             9324..10133
                     /locus_tag="VQ495_001150"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2531113.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10609..11589
                     /locus_tag="VQ495_001151"
                     /pseudo
     CDS             10609..11589
                     /locus_tag="VQ495_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11614..12126
                     /locus_tag="VQ495_001152"
     CDS             11614..12126
                     /locus_tag="VQ495_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2531114.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPISAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3606 a         2232 c         2769 g         3611 t
ORIGIN      
        1 tgctcttgtt tcaattgact atagcagatt gaaataagat gagaacaaat cgattgggaa
       61 agtaaaatta atttctataa atgttttagc aattgtttcg tactatttca gattcagtct
      121 actatatatc ataatggaca aagggcaagc tgtcaagaaa ttacagttat tttagtaaaa
      181 aaagaagcag aaaaatttaa ttttctactc cttttctata ggatttattt ttctttttca
      241 gaacttttaa cttcagcttt tttggcagat ttaatctgaa tgtttccctt gctagtcata
      301 actgctttga gatctttttc gcttggattt tcaaggtagt agtcagtgat tgtgtcctca
      361 atatagtctt gaatagtccg acgaagtggg cgtgctccca tttttggatc ataacctagg
      421 tcaaccaact tttccttgac cttgtcagtt acatccaaac gaatgttgtt gctagagagg
      481 cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc cttgctgaga
      541 gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa gaagttaccg
      601 agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc aacgctagct
      661 tcggtctttc ctgtacctgc atttgaggtc atgataatga tggcatcctt gaagctaacg
      721 gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa catgtgcata
      781 acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg attgtggcga
      841 actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg agcgccgacc
      901 aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat catactatca
      961 gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc gacaccagtt
     1021 ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc gacacgatta
     1081 cggcgaatag ccttggcaat cttatcgact gcatcatctt ggccaataac atgagacttg
     1141 agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc accaacaggg
     1201 atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat aggagtatcc
     1261 tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc gcggaagtag
     1321 gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc agcctcaatc
     1381 aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat cttagaacca
     1441 gcttcatcta ggaggtcaat ggccttgtca ggcaagaagc gatcttggat gtagcgattg
     1501 gaaagagttg cagctgcttc aatcgcagca tctgtatatt gaacgtggtg gtaatcttcg
     1561 tatttctttt gaatcccttt gagaatagtg attgtttcgt ccaccgttgg ttcatcgact
     1621 ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg gtattcattg
     1681 agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt gaggatattt
     1741 cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg gatttcatcg
     1801 ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg catgcgttct
     1861 tcaaattgtc ctcgaatccc cgttccttga actaagctaa ccacatccag acggatgact
     1921 tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc tagaccttcg
     1981 acaacggccg tttttccgac accaggttca ccgataagga caggattatt cttggttcta
     2041 cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac ggggtcaatg
     2101 tctccacgac gggcaatttc agttacatta ataccaaatt cttccagcag gcctttttct
     2161 tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc gccgtttcca
     2221 ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga aggtctgaaa
     2281 ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt cagatccgtc
     2341 atacctttga agaggctatt gttaggatct gttttgataa tcttatagca gttttgacag
     2401 aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt tgagtcgtta
     2461 attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc tgacttgttt
     2521 aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg tgtaaagcaa
     2581 gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa tttggactat
     2641 aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg acaatcccag
     2701 cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc aatgctatca
     2761 aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc gttttcttcg
     2821 tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt acgttttaaa
     2881 tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa atgtgattcc
     2941 attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg ccaacttgac
     3001 tagaaaaaac taagatttct cgctaattct gctagtttaa agatttttaa ataaattgga
     3061 taaaaaattt aaattttaat tcaaaacatg ttataatcat acagtattct attttagaaa
     3121 gcagtgtgac tatgaatttt tcttttttac ctaagtattt accttatttt aactatgggg
     3181 ctgttgtgac gattcttatt tctatctgtg ttatcttttt gggaactatt ttgggtgttg
     3241 tcttggcttt tgggcaacgt tcaaagttta aaccgcttgt ttggttggcc aacttgtacg
     3301 tttggatttt ccgtgggaca ccgatgatgg ttcaaattat gattgccttt gctcttatgc
     3361 atatcaatgc tccgactatt cagattggaa ttttaggtgt tgatttttcg cgtctgattc
     3421 cagggatttt gattatctct atgaatagtg gtgcttatgt ttcggagatt gttcgtgccg
     3481 gaatcaatgc ggttccaaaa ggtcagctag aggcggctta ttcgctaggg attcgtccta
     3541 aaaatgcgat gcgttatgtg attttgccac aagcagtcaa aaatatcttg ccagcattgg
     3601 ggaacgaatt tatcaccatt atcaaggaca gctccctctt atcagctatt ggggtcatgg
     3661 agttgtggaa tggggctaca acagtttcta caacaaccta tctaccttta acaccacttt
     3721 tatttgcagc attttactac ttgattatga cctctattct gacagtagcc ttgaaagctt
     3781 ttgaaaaaca tatgggacaa ggagataaga aataatgaca gaaaccttga taaaaattga
     3841 aaatttacat aaatcttttg gaaagaatga agtattgaag ggcatcaacc tcgagattaa
     3901 aagaggagaa gttgtcgtta tcatcggtcc ttcagggagt gggaaatcta ccttgcttcg
     3961 ctctatgaat ttgttggagg aagcaaccaa ggggaaggtt atctttgagg gagtcgatat
     4021 tacggacaag aagaatgacc tgtttgccat gcgtgagaag atgggcatgg ttttccaaca
     4081 atttaacctc tttcctaata tgactgtgat ggaaaatatt accttgtcac ctatcaagac
     4141 taaaggtgaa agcagggaag ttgctgagaa gagagcccaa gagcttttgg aaaaagttgg
     4201 tttgccagat aaggcagacg cttatccaca gagtctgtcg ggtgggcaac aacaacggat
     4261 tgccatcgcg cgtgggttgg ctatggaacc agatgttttg ctctttgacg aaccaacttc
     4321 agccttggat cctgaaatgg taggtgaggt attggctgtt atgcaagacc ttgccaagtc
     4381 agggatgact atggttatcg taacacatga gatgggattt gcccgtgagg tggcagatcg
     4441 tgttatcttt atggcagacg gtgtggttgt tgaagatggg acacctgagc agatttttga
     4501 acaaacccaa gaacaacgga ctaaagattt cttgagtaag gttttataat ctattttaaa
     4561 atttcaagac aagattagtg aaaagttcaa ccagagcttt ttcttatagt ttgaaactat
     4621 aggataacct aggaaagaag tgttagaggg cactagcagt ttttggtata atggaagata
     4681 tttgaaagaa aagagaagtg atatgacaca gattattgat gggaaagctt tagcggccaa
     4741 attgcagggg cagttggctg aaaagactgc aaaattaaag gaggaaacag ctctagtgcc
     4801 tggtttggta gtgattttgg ttggggataa tccagccagc caagtctacg ttcgcaacaa
     4861 ggagaggtca gctcttgcgg ctggttcccg tagcgaagta gtgcgagttc cagagaccat
     4921 tactcaagag gaattgttag acctgattgc taaatataat caggatccag cttggcatgg
     4981 gattttggtc cagttgccat taccaaaaca tatcgatgaa gaggcggttt tattagccat
     5041 tgacccagaa aaggatgtgg atggtttcca tcccctaaac atgggacgtc tttggtctgg
     5101 acatccagtc atgattccct cgacacctgc aggaattatg gaaatgtttc atgaatatgg
     5161 gattgacttg gaaggtaaaa atgcggtcgt aatcggtcgt tcaaatatcg ttggaaaacc
     5221 tatggctcag cttcttttgg ctaaaaatgc gacagtaacc ttgacccact cacgaactca
     5281 taatcttgcc aaggtggctg ctaaagcaga tattcttgta gtcgcaatcg gccgcgccaa
     5341 gtttgtgact gctgactttg tcaaaccagg tgcggttgtc attgacgttg ggatgaatcg
     5401 agatgaaaat gggaagctct gtggagatgt tgattatgag gcagttgcac cacttgctag
     5461 ccatatcacg ccagtccctg gaggtgtcgg tcctatgacc attaccatgc tgatggagca
     5521 aacttatcaa gcagcacttc ggacattgga tagaaaataa gagaagcatt cgattaatag
     5581 agtgtatttt ctatagctat atctaaaatg gcagaaatga atattaaatt ttagatataa
     5641 gattacaaaa ggaggtctgc gtctcctttt tattgtataa taaaagtgag aggaaaaaag
     5701 gatgaaagtg attaatcaaa ccttattaga aaaagttatt attgaacgtt ctcgttcaag
     5761 tcataaagga gattatggtc gtctgctgct gcttggtggt acttatcctt atggaggtgc
     5821 catcatcatg gctgctttag cagctgtaaa aagcggtgca ggattgatga ccgttggaac
     5881 ggacagggaa aatatcccgg ctctgcacag ccatttacct gagcctatgg ccttttctct
     5941 tcaagaccag caattgttaa aagagcaatt ggagaaggca gaagttatct tgctggggcc
     6001 tggtttagga gacaatgctt ttggagaaga tttagttaaa caggtctttg ctggcttaaa
     6061 acagaatcag attttgattg tagacggagg tgccttgacc attcttgcta ggacaagttt
     6121 gtcatttccc tcaagccagc ttatcctaac tcctcaccaa aaagaatggg aaaaattatc
     6181 tggaattacg attgaaaagc aaaaggaaga tgcaacggct agtgttctga cttccttccc
     6241 tcaaggaaca attttggtgg agaaaggccc agctactcgt atttgggaag ttggccaatc
     6301 tgattattac cagttacagg ttggtggtcc ttatcaggcg actgggggaa tgggagatac
     6361 tctggctggg atgatcgcag gatttgtagg ccaattccga caggccagtc tctacgagcg
     6421 tgtggcagta gcgacccatc ttcattcagc catagcccaa gaactatctc aagaaaatta
     6481 tgtggtctta ccgacggaaa tcagcaggta tcttcccaaa ataatgaaaa ttatttgtca
     6541 acaagagagg gtaagcaaag ataaacttgt ttaaaataga cagataaatc tacttccttc
     6601 tcttaattta tgattcttga aatgcgcctg ctttaaggct aaactagacg tatcaaagtt
     6661 aagagtttgt ttttgtaact ggaaagagat gaacaaatca aatacataaa agataaactc
     6721 atttttattc gttttgtaaa aataaaatgc atatttttaa agaaagattg atgaaaaatg
     6781 tagatttgat gtttttactt gaataaaaag cggtttcata gtaaaataag aactgagaat
     6841 gatggaggta aatcggtgga aaatctgaag aaaatggcag gtatcaaggc tgctgagttc
     6901 gtgagcgatg gaatggtcgt tggacttgga acaggctcga ctgcctatta ttttgtcgaa
     6961 gaaatcggtc gtcgtatcaa ggaagaagga ttgcagatta cagctgtaac aacttctagt
     7021 gtgaccacta aacaggcaga aggccttaat atcccgctca agtctattga ccaagtagac
     7081 tttgtcgatg tgacggttga tggcgcggat gaagtggata gtcagtttaa tggaatcaaa
     7141 ggcggtggtg gtgcccttct catggaaaag gtggtcgcaa caccatcaaa agaatacatt
     7201 tgggtggtgg atgaaagcaa gctggtcgaa aaactaggtg cttttaaatt gccagtagaa
     7261 gtggttcagt atggtgcaga gcaggtcttt cgtcattttg aacgagctgg ctacaaacca
     7321 agtttccgtg aaaaagacgg tcaacgtttt gtgacggata tgcagaattt tatcattgac
     7381 ctcgccttgg atgtcattga aaatccaatt gcttttggac aagaattgga ccatgtcgtt
     7441 ggtgttgtgg agcatggttt attcaaccaa atggtggata aggtaatcgt tgctggacga
     7501 gatggagttc agatttcaac ttcaaaaaaa ggaaaataga agggggcata agatgtctaa
     7561 atttaatcgt attcatttgg tggtactgga ttctgtagga atcggtgcag caccagatgc
     7621 taataacttt gtcaatgcag gggttccaga tggagcttct gacacactgg gacacatttc
     7681 aaaaacagtt ggtttgaatg tcccaaacat ggctaaaata ggtcttggaa atattcctcg
     7741 tgaaactcct cttaagactg tagcagctga aagcaatcca actggatatg caacaaaatt
     7801 agaggaagta tctcttggta aggatactat gactggacac tgggaaatca tgggactcaa
     7861 cattactgag cctttcgata ctttctggaa cggattccca gaagaaatcc tgacaaaaat
     7921 cgaagaattc tcaggacgca aggttattcg tgaatccaac aaaccttatt caggaacggc
     7981 tgttatcgat gattttggac cacgtcagat ggaaactgga gagttgatta tctatacttc
     8041 agctgaccct gttttgcaga ttgctgccca cgaagacatt attcctttgg atgaattgta
     8101 ccgtatctgt gaatacgctc gttcgattac ccttgagcgt cctgcccttc ttggtcgcat
     8161 cattgctcgc ccttatgtag gtgaaccagg taacttcact cgtacggcaa accgtcgtga
     8221 cttggctgta tctccatttt ccccaactgt tttggataaa ttgaatgagg ctggtatcga
     8281 tacttatgct gtgggtaaaa tcaacgatat ctttaacggt gctggtatca accatgacat
     8341 gggtcacaac aagtcaaata gtcatggaat tgatacacta ttgaagacta tgggacttgc
     8401 tgagtttgaa aaaggattct cattcacaaa cctagttgac tttgatgccc tttacggcca
     8461 tcgtcgtaat gctcacggtt accgtgattg cttgcatgag tttgatgaac gcttacctga
     8521 aattatcgca gctatgagag agaatgacct tctcttgatt actgcggacc atggaaatga
     8581 cccaacgtat gcaggaacgg atcacactcg ggaatatatt ccattgttgg cctatagccc
     8641 tgcctttaaa ggaaatggtc tcattccagt aggacatttt gcagatattt cagcgactgt
     8701 tgccgataac tttggtgtgg aaactgctat gattggggaa agtttcttag ataaattggt
     8761 ataagatgac gcgctatgct ttgctggtga gaggtatcaa tgttggtggt aagaataagg
     8821 tcgtcatggc ggagcttcgt caagaattga caaacttggg actggaaaag gttgagagct
     8881 acatcaatag tggcaatatt ttctttactt cgatagattc caaagcccaa ttggttgaaa
     8941 agctagagac tttctttgca gtccattatc catttattca gagcttttct ttactgagtc
     9001 tagaggactt tgaggcggaa cttgaaaatc taccagcttg gtggagcaga gacttggcac
     9061 gaaaagattt tctcttttac actgagggtt tggatgtgga ccaagtcatc gcgacagttg
     9121 aaagtttaga gctgaaagat gaagtgcttt attttggaaa acttgggatt ttctggggga
     9181 aattttctga agaatcctat tctaagactg cctatcataa gtacttgctg aaggtgcctt
     9241 tctaccgcca cattactatt cgtaatgcta aaacctttga caaaattggt caaatgctaa
     9301 aaaaataata aaggagacac acaatgacat ttttaaacaa aatccatgaa actgctactt
     9361 tcctgaaaga aaagggaatt gcagcccctg agttcggtct aatccttgga tcaggacttg
     9421 gagaattggc agaagaaatc gaaaatccag ttgtagtaga ctatgctgag attccaaact
     9481 ggggccgttc aacagtagtc ggtcatgctg gtaaattggt atatggtgaa ctggcaggtc
     9541 gcaaggtctt ggctcttcaa gggcgtttcc atttctatga agggaatcct ctggaagtgg
     9601 tgactttccc agttcgtgtg atgaaagttc ttggatgtga aggtgttatt gtaaccaatg
     9661 cagctggcgg tatcggattt ggtcctggta ccttgatggc tatctcagac catatcaaca
     9721 tgacggggca aaatccattg atgggtgaaa acttggatga ctttggccca cgtttcccag
     9781 atatgtctag ggcctacaca ccagaatacc gtgccactgc ccatgaagtg gctaaaaaac
     9841 ttaatatcaa gcttgatgaa ggtgtctata tcggagttac tggtccgact tatgaaacac
     9901 cagcagaaat tcgttcctat aagacactgg gagcagatgc agttggtatg tctacggttc
     9961 ctgaagttat cgtggcagcc cactctggct tgaaagttct gggaatttca tgtatcacta
    10021 actttgcggc cggtttccaa gaagaactca atcacgaaga agttgtagaa gtgactgaac
    10081 gtgttaaagg tgatttcaaa ggcttgctta aagcgattct tgctgaattg taagaaaaaa
    10141 gatttaaaag ggggagtgcc tctgtttttt caggattgac tgcctatccg gattaaagaa
    10201 gaaacagagg aatactatga gcttcttcct gctcttataa ctgaaagaag cggaagaata
    10261 ggtatgtctg atctgatagc cagcattgtg aaagacaaga ttctaggata ctagcattag
    10321 cttcctagcc aagcagacta gtatgataag gagagatgag aatgaattga ctttctgaat
    10381 ttctcagtct tatcatatat agcacaatga gatttcgctt gagtctgctt gtaaataaac
    10441 gaaaagaaag ataagaaata atgaaaattg gtcaacgaat tatgcgcttt ggcataaaaa
    10501 attaagtatc ggagttgtat ctgttgtagt cggctttgat ttctagctcc agctggaatt
    10561 tcagccaatg aagtaaagca agatgtaaca tctgaagtgg taataggtgt gctagattct
    10621 aaggaggaat tgaaagagtc agaaaatgat gctccaaaac tagaaactcc tcttagagag
    10681 gagccaagac tagctcctca aacgcttccg gaagcaagtg aagttcttga aaacaaaagg
    10741 gaagagtcaa aagtagagat aacataacca gctcaagcgg atgatatccg caaggttgtt
    10801 ggggaattag ccaaggatat aagtattact aagttgtata tgacaggtca ttctcttgga
    10861 tgttacctag ctcagattgc agcggttgaa gcttaccaaa aatatcctga tttttataac
    10921 catgtattga ggaaagtgac aactttcagt gctcctaaag tgattacttc cagaactgtt
    10981 tggaatgcta agaatggttt ctgggatgtt ggtttggaaa gtcgtaaatt agctgttagt
    11041 ggaaaaatta agcattatgt ggttgataat gacaatgttg tgactccctt gattcataat
    11101 aatcgtgata ttgttacatt tacaggtaat tcacgcttta aacaccgttc tcgtggctat
    11161 tttgaaagtc caatgaatga tattcctaac tttaatattg gtaaacaagc taccttggat
    11221 aaacatggtt atcgtgatcc gaaattggat aaagtgcgat tctttaagaa acaggctctg
    11281 cctcgatctt ctagtcaacc aagcgctgaa ccaatggaaa atattgcctt aggaaaacag
    11341 gttactcaaa gttcgacagc tttcggagga gatgctagaa gagctgtgga tggcaaagtc
    11401 gatggtaact atggtcacaa ttctgtcact catacaaact tccaatctaa gccttggtgg
    11461 caagtagatt tggctaaaga agaaaccatt cgccaaatca atatttacaa ccgaacagac
    11521 actgcccagg atagattggc aaactttgat gtcattcttt tagacagttc tggtaaagaa
    11581 attgagtgaa aacgtataat atctcctaaa gatgtgtcag cacaaattac gattaaccat
    11641 aaaaaagcgc gctatgttcg gattgagcta gaaggctata atgccctcag tcttgcagaa
    11701 gttgaagttt tccgctttat agctacgaat gctgaaacgg cgacacaagt ttctaagcca
    11761 gttcaaccaa tcagtcagac tcctgtgaaa gataaaacat tgacaattca acacagtgga
    11821 gcttacattg cccgctactc cataacttgg gaagaagttc cagtagataa agatggaaac
    11881 caagttgttc gtagtcattc ttgggaagga aacggtcgca accagactgc gggttttgtc
    11941 ctcaacctcc caatcaaaga aaatatgaga aatctgcgag ttaagattga gaaaaagacg
    12001 ggcctactat ggaatagatg gcaaacaatc tatgaaaaca gaccaatttc agctcaaccc
    12061 caccgtaaaa ttacccattg gggtacgaca ttgaattcca aggtgagtga cgatgatgtc
    12121 ttgtaatctg atggtacaat gacagttagt ttgtctagtt tataagaaag tactacctga
    12181 gcttgaatag aactcaggta gctctctgtg aaagaaca
//
LOCUS       DAVBCG010000050        11954 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBCG010000050 DAVBCG010000000
VERSION     DAVBCG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.050"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(101..1732)
                     /locus_tag="VQ495_001153"
     CDS             complement(101..1732)
                     /locus_tag="VQ495_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2531115.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2025..7004
                     /locus_tag="VQ495_001154"
     CDS             2025..7004
                     /locus_tag="VQ495_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2531116.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLIALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7094..8290
                     /locus_tag="VQ495_001155"
     CDS             7094..8290
                     /locus_tag="VQ495_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2531117.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8426..8953
                     /locus_tag="VQ495_001156"
     CDS             8426..8953
                     /locus_tag="VQ495_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2531118.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRVRLFLEKKEK"
     gene            9030..9386
                     /gene="glnR"
                     /locus_tag="VQ495_001157"
     CDS             9030..9386
                     /gene="glnR"
                     /locus_tag="VQ495_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2531119.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRCALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9423..10769
                     /gene="glnA"
                     /locus_tag="VQ495_001158"
     CDS             9423..10769
                     /gene="glnA"
                     /locus_tag="VQ495_001158"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2531120.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10865..10984)
                     /locus_tag="VQ495_001159"
     CDS             complement(10865..10984)
                     /locus_tag="VQ495_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2531121.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11242..11379
                     /locus_tag="VQ495_001160"
     CDS             11242..11379
                     /locus_tag="VQ495_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531122.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
     gene            11472..11954
                     /locus_tag="VQ495_001161"
                     /pseudo
     CDS             11472..11954
                     /locus_tag="VQ495_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3841 a         2184 c         2565 g         3364 t
ORIGIN      
        1 tactatatat ctgcagaata ctctttcagt ttcattcagc ctagtcttgc ttaggaaaaa
       61 gtaaaaagcc atccaattat gatggatagc ttcttggttc ttagatttgt tctgcaaaaa
      121 ccttttcagc aaggttcata gcgtggtctg atacacgagt gtagtgtgag atgatgtcga
      181 taaagttgac cccagcttgt gttgaacatt cgcctttgtt gaggcgtttg atgtgggttt
      241 ttctgagaac acgttctatc ttattgattg cttcatgacg ttctacaaga ctgcgtgctt
      301 tttcaatatc attgttttcc acactatcca gagcatcttt gataaagtca ctagtttggc
      361 ggtaaacttc ctctaattct ttcaaggcgg catcagaaaa ttcaacattt ttccgttgaa
      421 gatagtcagt cagattgagt agagcctccg tgtggtctcc aatccgttcc aaatcacggg
      481 aggaatcaag gatattggta agcacttcac tttctttttg gctgagagct tcgcttgaaa
      541 gggcaatgag ataacgtgtt aattgctcat cgatggtgtt aattgcttct tcggttttat
      601 gccctttttc agcaactttt tcatccaagt caatgatgta cttataggaa aggtcaaagg
      661 ctttagcagc gtagtttcct aagtgcaaga gctctttctt agcatttcct agagcgatag
      721 atggggcctg tttgatgaaa tgttcatcaa gatataaggg ttcgtatttg acaacctcgt
      781 cctctccagg aataatcttg gttacaaagt aagccagagc tccgataaat ggaaattgga
      841 caatggtgtt ggtaatatta aaggttccgt gagcaaaggc gatggtcatt tccggtgcta
      901 gatttagcgt agcttcaaac caatgaatca ggacagtaaa aggaactaga aaaataacgc
      961 agacgactgt tccgataacg ttgaaggcaa catgagctcc tgctacccgt ttagctgcaa
     1021 tattagcccc taaagaggca atgatggctg taatggttat cccgatattg tcaccaaata
     1081 gaactggcaa agctccctgt aggtcaatta gattgccggc gtagaggttt tgtaaaatcc
     1141 caatggtagc cgaagaagct tgaattagca aggtcaagcc ggtaccgaca aagacaccca
     1201 aaacaggatt cttacttagc tcaatcatat agtccttaaa gacctgtaaa tccttgagtg
     1261 gagccattgc gccgctcatg agattgaggg caaaaaagat accaccgaca ccaaagagga
     1321 tgcgtccgat attattgact gtccgatttt ttgtaaaaaa gagacagacg gcaccgataa
     1381 agagcatagg tagggcatag ttacctaatt taaaaccgat gagaaaggat gtgacagttg
     1441 tcccaatatt agcacccatg acaatcccga tagcctgacg taaggttaag agaccggcac
     1501 tgaccaggcc gactgtgata actgttacac cagaactaga ctgaattaga gcagtcatcc
     1561 caataccaac cagaactcca aagaaaggat tactagtata tttgtcaatg taaaaacgaa
     1621 ggcgatctcc agcagcttgt tgcaaaccgt ctcccatggt cttgatgcta tataagaata
     1681 gccccagacc acctaaaaag tgaaataaaa tttcctgcca attaatggac atttcttttt
     1741 cctccgaaaa ataatcgcgg aatatcttct attctatttt aaaggataaa agtaaatcta
     1801 acaagtgtta atgtcaagtt tgtaaaagaa aattataatt gataagtgta ttatttttaa
     1861 gagtttttaa ccttatttta gatttgtgca aacagttatt gttttttgtc taaaaatgtt
     1921 ttaggataaa aatgtgatta tcatggttct agagtttgat aaaaaactac agaatataaa
     1981 gcgctttctt gaaaacaaca aaatcaatct tttaggagga gaaaatgaag aatccatttt
     2041 ttgaaagacg ttgtcgttac agtattcgta agttatcagt aggagcctgc tcgctgatga
     2101 ttggtgctgt tttatttgct ggtccagcct tggctgaaga aactgcagtt cctgaaaata
     2161 gcggagctaa tacagagctt gtttcaggag agagtgagca ttcgaccaat gaagctgata
     2221 agcagaatga aggggaacat gctagagaaa acaagctaga aaaggcagaa ggagtagcga
     2281 tagcatctga aactgcttcg ccagcaagca atgaagctgc aactactgaa actgcagaag
     2341 cagctagcgc agctaaacca gaggaaaaag caagtgaggt ggttgcagaa acaccatctg
     2401 cagaagcaaa acctaagtct gacaaggaaa cagaagcaaa gcccgaagca actaaccaag
     2461 gggatgagtc taaaccagca gcagaagcta ataagactga aaaagaagtc cagccagatg
     2521 tccctaaaaa tacagaaaaa acattaaaac caaaggaaat caaatttaat tcatgggaag
     2581 aattgttaaa atgggaacca ggtgctcgtg aagatgatgc tattaaccgc ggatctgttg
     2641 tcctcgcttc acgtcggaca ggtcatttag tcaatgaaaa agctagcaag gaagcaaaag
     2701 ttcaagcctt atcaaacacc aattctaaag caaaagacca tgcttctgtt ggtggagaag
     2761 agttcaaggc ctatgctttt gactattggc aatatctaga ttcaatggtc ttctgggaag
     2821 gtctcgtacc aactcctgac gttattgatg caggtcaccg taacggggtt cctgtatacg
     2881 gtacactctt cttcaactgg tctaatagta ttgcagatca agaaagattt gctgaagctt
     2941 tgaagcaaga cgcagatggt agcttcccaa ttgcccgtaa attggtagac atggccaagt
     3001 attatggcta tgatggctat ttcatcaacc aagaaacaac tggagatttg gttaaacctc
     3061 ttggagaaaa gatgcgccag tttatgctct atagcaagga atatgctgct aaggtaaacc
     3121 atccaatcaa gtattcttgg tacgatgcca tgacctataa ctatggacgt taccatcaag
     3181 atggtttggg agaatacaac taccaattca tgcaaccaga aggagataag gttccggcag
     3241 ataacttctt tgctaacttt aactgggata aggctaaaaa tgattacact attgcaactg
     3301 ccaactggat tggtcgtaat ccttatgatg tatttgcagg tttggaattg caacagggtg
     3361 gttcctacaa gacaaaggtt aagtggaatg acattttaga cgaaaatggg aaattgcgcc
     3421 tttctcttgg tttatttgcc ccagatacca ttacaagttt aggaaaaact ggtgaagatt
     3481 atcataaaaa tgaagatatc ttctttacag gttatcaagg agaccctact ggccaaaaac
     3541 caggtgacaa agattggtat ggtattgcta acctagttgc ggaccgtacg ccagcggtag
     3601 gtaatacttt tactacttct tttaatacag gtcatggtaa aaaatggttc gtagatggta
     3661 aggtttctaa ggattctgag tggaattatc gttcagtatc aggtgttctt ccaacatggc
     3721 gctggtggca gacttcaaca ggggaaaaac ttcgtgcaga atatgatttt acagatgcct
     3781 ataatggcgg aaattccctt aaattctctg gtgatgtagc cggtaagaca gatcaggatg
     3841 tgagacttta ttctactaag ttagaagtaa ctgagaagac caaacttcgt gttgcccaca
     3901 agggaggaaa aggttctaaa gtttatatgg cattctctac aactccagac tacaaattcg
     3961 atgatgcaga tgcatggaaa gagctaaccc tttctgacaa ctggacaaat gaagaatttg
     4021 atctcagctc actagcaggt aaaaccatct atgcagtcaa actatttttc gagcatgaag
     4081 gtgctgtaaa agattatcag ttcaacctcg gacaattaac tatctcggac aatcaccaag
     4141 agccacaatc gccgacaagc ttttctgtag tgaaacaatc tcttaaaaat gcccaagaag
     4201 cggaagcagt tgtgcaattt aaaggcaaca aggatgcaga tttctatgaa gtttatgaaa
     4261 aagatggaga cagctggaaa ttactaactg gctcatcttc tacaactatt tatctaccaa
     4321 aagttagccg ctcagcaagt gctcagggta caactcaaga actgaaggtt gtagcagtcg
     4381 gtaaaaatgg agttcgttca gaagctgcaa ccacaacctt tgattggggt atgactgtaa
     4441 aagataccag cctaccaaaa ccactagctg aaaatatcgt tccaggtgca acagttattg
     4501 atagtacttt ccctaagact gaaggtggag aaggtattga aggtatgttg aacggtacca
     4561 ttactagctt gtcagataaa tggtcttcag ctcagttgag tggtagtgtg gatattcgtt
     4621 tgaccaagcc acgtaccgtt gttagatggg tcatggatca tgcaggagct ggtggtgagt
     4681 ctgttaacga tggcttgatg aacactaaag actttgacct ttattataaa gatgcagatg
     4741 gtgagtggaa gctagctaag gaagtccgtg gtaacaaagc acacgtgaca gatatcactc
     4801 ttgataaacc aatcactgct caagactggc gcttgaatgt tgtcacttct gacaatggaa
     4861 ctccatggaa ggctattcgt atctataact ggaaaatgta tgaaaagctt gatactgaga
     4921 gtgtcaatat tccgatggcc aaggctgcag cccgttctct aggcaataac aaggtacaag
     4981 ttggctttgc agatgtaccg gctggagcaa ctattaccgt ttatgataat ccaaattctc
     5041 aaactccgct cgcaaccttg aagagcgaag ttggaggaga cctagcaagt gcaccattgg
     5101 atttgacaaa tcaatctggt cttctttatt atcgtaccca gttgccaggc aaggaaatta
     5161 gtaatgtcct agcagtttcc gttccaaaag atgacagaag aatcaagtca gtcagcctag
     5221 aaacaggacc taagaaaaca agctacgccg aaggggagga tttggacctt agaggtggtg
     5281 ttcttcgagt tcagtatgaa ggaggaactg aggacgaact cattcgccta actcacgcag
     5341 gtgtatcagt atcaggtttt gatacgcatc ataagggaga acagaatctt actctccaat
     5401 atttgggaca accagtaaat gctaatttgt cagtgactgt cactggccaa gatgaagcaa
     5461 gtccgaaaac tattttggga attgaagtaa gtcaggaacc gaaaaaagat tacctagttg
     5521 gtgatagctt agacttgtct gaaggacgct ttgcagtggc ttatagcaat gacaccatgg
     5581 aagaacattc ctttactgat gagggagttg aaatttctgg ttacgatgct caaaagactg
     5641 gtcgtcaaac cttgacgctt cgttaccaag gtcatgaagt caactttgat gttttggtat
     5701 ctccaaaagc agcattgaac gatgagtacc tcaaacaaaa attagcagaa gttgaagctg
     5761 ctaagaacaa ggtggtctat aactttgctt catcagaagt aaaagaagcc ttcttgaaag
     5821 caattgaagc ggccgaacaa gtgttgaaag accatgaaac tagcacccaa gatcaagtca
     5881 atgaccgact taataaattg acagaagctc ataaagctct gaatggtcaa gagaaattta
     5941 cggaagaaaa gacagagctt gatcgcttaa caggtgaggt tcaagaactc ttggctgcca
     6001 aaccaaacca tccttcaggt tctgccctag ctccgcttct tgagaaaaac aaggccttgg
     6061 ttgaaaaagt agatttgagt ccagaagagc ttacaacagc gaaacagagt ctaaaagatc
     6121 tgattgcttt attgaaagaa gacaagccag cagtcttttc tgatagtaaa acaggtgttg
     6181 aagtacactt ctcaaataaa gagaagactg tcatcaaggg tttgaaagta gagcgtgttc
     6241 aagcaagtgc tgaagagaag aaatactttg ctggagaaga tgctcatgtc tttgaaatag
     6301 aaggtttgga tgaaaaaggt caagatgttg atctctctta tgcttctatt gtgaaaatcc
     6361 caattgaaaa agataagaaa gttaagaaag tatttttctt acctgaaggc aaagaggcag
     6421 tagaattggc ttttgaacaa acggatagtc atgttatctt tacagcacct cactttactc
     6481 attatgcctt tgtttatgaa tctgctgaaa aaccacaacc tgctaaacca gcaccacaaa
     6541 acacagtcct tccaaaacct acttatcaac cgacttctga tcaacaaaag gctcctaaat
     6601 tggaagttca agaggaaaag gttgcctttc atcgtcaaga gcatgaaaat actgagatgc
     6661 tagttgggga acaacgagtc atcatacagg gacgagatgg actgttaaga catgtctttg
     6721 aagttgatga aaacggtcag cgtcgtcttc gttcaacaga agtcatccaa gaagcgattc
     6781 cagaaattgt tgaaattgga acaaaagtaa aaacagtacc agcagtagta gctacacagg
     6841 aaaaaccagc tcaaaataca gcagttaaat cagaagaagc aagcaaacaa ttgccaaata
     6901 caggaacagc tgatgctaat gaagccctaa tagcaggctt agccagcctt ggtcttgcta
     6961 gtttagcctt gaccttgaga cggaaaagag aagataaaga ttaaatatcg aaaaatcttg
     7021 tgaaatcttt ccttatattt ccaaagtgtg atataatagt ttcgaataaa ataaataaag
     7081 gagtttttgt aacatggcaa aacttactgt taaagacgtt gacttgaaag gtaaaaaagt
     7141 cctcgttcgt gttgacttca acgtaccatt gaaagatggc gtaatcacta acgataaccg
     7201 tatcacagca gctcttccaa ctattaagta catcatcgaa caaggtggac gtgcaattct
     7261 tttctctcac cttggacgtg tgaaagaaga agctgataaa gctggtaaat cacttgctcc
     7321 tgtagcagct gacttggcag caaaacttgg tcaagatgtt gttttcccag gtgtcactcg
     7381 tggtgctgaa ttggaagcgg caatcaacgc tcttgaagat ggacaagttc tcttggttga
     7441 aaacactcgt tacgaagatg ttgacggcaa gaaagaatct aaaaacgatc ctgaacttgg
     7501 taaatactgg gcatcacttg gagatggtat cttcgtaaac gatgcattcg gtacagctca
     7561 ccgtgcacac gcatctaacg ttggtatctc agcaaacgtt gaaaaagcag ttgctggttt
     7621 ccttcttgaa aacgaaattg cctacatcca agaagcagtt gaaactccag aacgtccatt
     7681 cgtggctatc cttggtggtt caaaagtttc agacaagatc ggtgttatcg aaaacttgct
     7741 tgaaaaagct gataaagtcc ttatcggtgg tgggatgact tacacattct acaaagcaca
     7801 aggtatcgaa atcggtaact cacttgtaga agaagacaaa ttggatgttg cgaaagctct
     7861 tcttgaaaaa gcaaatggta aattgatctt gccagttgac tcaaaagaag ctaacgcatt
     7921 tgctggttac actgaagtgc gtgacactga aggtgaagca gtttctgaag gcttccttgg
     7981 tcttgacatc ggtccaaaat ctatcgccaa atttgacgaa gctttgactg gtgccaaaac
     8041 agttgtatgg aacggaccta tgggtgtatt tgaaaaccca gatttccaag ctggtacaat
     8101 cggtgtgatg gacgctatcg tgaaacaacc aggagttaaa tcaatcatcg gtggtggtga
     8161 ctcagctgcc gcagcgatta accttggccg tgcagacaag ttctcatgga ttagtacggg
     8221 tggtggagca tcaatggaac ttcttgaagg taaggttctt ccaggacttg cagccttgac
     8281 agaaaaataa gattttataa acaaatcaaa gaagagaggg atgaaagttc ctcttttctt
     8341 ttgcttaaaa taaaaacgct tcctctcaac tattactcat aaaaatcacc gatttatgat
     8401 aaaatggaaa tagaaagttg agattatgag ttattttaaa aaatataaat tcgataaatc
     8461 ccagttcaaa cttggtatgc gaacctttaa aacaggtatt gctgtttttc tagttctctt
     8521 gatttttggc ttttttggct ggaaaggtct tcaaattggt gctttgacag ccgtttttag
     8581 cctgagggag agttttgatg agagtgttca ttttgggact tcgcgtattc taggaaatag
     8641 tatcggtgga ctctatgcct tggtcttctt cttattaaat acctttttcc acgaagcctt
     8701 ttgggtgacc ttggtagttg ttccaatctg caccatgtta accattatga caaatgtagc
     8761 catgaataac aaagcagggg ttattggtgg tgtagcagct atgttaatca ttaccctatc
     8821 aattccaagt ggtgagacaa tttcgtacgt gtttgtgcgt gtatcagaaa cgtttatggg
     8881 agtttttgtc gcaattatcg taaattacga tattgatcgt gttcgtctct ttttagagaa
     8941 aaaagaaaaa taatgttaca ttttataaca ttatcaattg acgtttgtct ttttttagac
     9001 tataatagac agaaagaagg aaattgtaaa tgaaggaaaa agaatttcgc cgaaatatgg
     9061 ctgtttttcc tatcggcagt gttatgaagt tgaccgatct atcggcgcgt cagattcgtt
     9121 attatgaaga tcaagagttg atcaagcccg atcgaaacga aggaaatcgt cgcatgtatt
     9181 ccttgaatga catggatcgt ctgcttgaaa tcaaagatta tatctctgaa ggttataata
     9241 tcgctgccat taagaaaaaa tatgctgaac gtgaagcgaa atccaagaaa gcggttagtc
     9301 agactgaagt acgttgtgca cttcacaatg aactcctcca acaggggcgt tttgcttcag
     9361 tacagtcacc ttttggtcgc ggttaggcaa ccgcaagtag tcatagatta aggagaaaac
     9421 ttatgccaat cacagctgca gatattcgtc gtgaagtcaa ggaaaaaaat gttaccttta
     9481 ttcgtcttat gttctcagat attttgggaa ccatgaaaaa cgtcgaaatt cctgctacag
     9541 atgaacagtt agataaggtc ttgtcgaaca aggttatgtt tgatggatct tctattgaag
     9601 gttttgtacg tatcaatgag tcggatatgt acttgtaccc ggacttggat acatggacag
     9661 tcttcccttg gggagatgaa aatggaagtg ttgcaggtct gatctgtgat gtctatacaa
     9721 cagaaggtga accatttgcg ggtgaccctc gtggtaattt gaaacgagct cttcgtcaca
     9781 tggaagaagt tggattcaaa tccttcaacc ttggtccaga gccagaattc ttcctattta
     9841 agttggatga aaatggggac ccaacacttg aagtgaatga caagggtggc tactttgatt
     9901 tggcacctac tgaccttgcg gacaacacac gtcgtgagat tgtgaatgtc ttgaccaaaa
     9961 tgggatttga agtagaagcg agtcaccacg aggttgcggt tggacagcat gagattgact
    10021 ttaagtacga tgaagttctc cgtgcttgtg ataagattca aatctttaag cttgttgtta
    10081 aaaccattgc tcgcaaacac ggactttacg caacatttat ggcgaagcca aaatttggta
    10141 ttgctggatc aggtatgcac tgtaatatgt ccttgtttga tgcagaagga aataacgcct
    10201 tctttgatcc aaatgatcca aaaggaatgc agttgtcaga aacagcttac catttcctag
    10261 gcggtttgat caagcatgct tacaactata ctgccatcat gaacccaaca gttaactcat
    10321 acaaacgttt ggttccaggt tatgaagcgc ctgtttacat tgcttgggct ggtcgtaacc
    10381 gttcgccact tgtgcgcgta cctgcttcac gtggtatggg aactcgtctt gagttgcgtt
    10441 cagtggatcc aatggcgaac ccttacattg ctatggctgt tcttttggaa gttggtttgt
    10501 atggtattga aaataaaatc gaagcaccag ctcctatcga agaaaatatc tacatcatga
    10561 cagcagaaga gcgcaaggaa gctggtatta cagaccttcc atcaactctt cacaacgctt
    10621 tgaaagcttt gacagaagat gaagtggtta aagctgctct cggagatcac atctacacta
    10681 gcttccttga agccaaacga atcgaatggg caagttatgc aaccttcgtt tcacaatggg
    10741 aaattgataa ttatttagac ctttactaat actaatatag aagaaagatt gcctgtgggt
    10801 aaacagtcac acagcagtct aagccatcaa atacctataa cccttaaaat agcgatttcg
    10861 gtcgctattt atttttcgaa aaatcctctt gactcttttg cgaattagat ttatcatcag
    10921 acgtgtagaa gaaaatcaag gagtcactat ggaaaaattc aatttcaaaa acaatatcgg
    10981 gcaagaaaac aagttacttc aaatcgaaat ctacaagttt actaactttt gcaagcttca
    11041 aaattacaca tctgtaaata ttttttcgaa agatatattt gaagccatag taaattgaga
    11101 ctagaatagt acactacatc tgctaaaaat ttctagaaat taatttaact ttcctaatcg
    11161 atttgtttac atcttatttc aatctactat attatctcaa taaatgatga tggcgactgt
    11221 tttgtttgtg cttgtcgatt tttgaatcct gatcgagcgg aagaatattt ctgccgaggt
    11281 tgtcaagggg aaaatcctga agatattgaa ttttatgatg aacaattaca ggcagaaaag
    11341 gtggaggctt tgaatattcg attggaagta aaaaattgaa ttatctacaa tctcatcaac
    11401 aataaaccta tttcaacccc attatctata ggcgtatttt tacgcctttt tatatacttg
    11461 aagcacataa gaaacattat gggaagtttg ctgatggaag tactcaagaa attgatgttc
    11521 cttatgatat tcctgatact tgggagtggg tgaggataaa atcaatttat tggaattttg
    11581 ggcaaaataa gccagagaaa tcctttaggt atatagatac gtctagtatt gatagaaaaa
    11641 agaacataat caactacaaa aatctacaat atctttcacc tgaacaagcg ccttcccgtg
    11701 ccagaaaatt agtttcgcag aatagtgtct tattttcaac agttagacca tatctaaaaa
    11761 atattgctgt agttagagaa cttaaagagt atttgatagc tagtacagca tttattgttt
    11821 tggatacttt acttaacgaa acatatttga aatattattt attatcagat aattttatca
    11881 atcgtgttaa taataaatct actggaacaa gttatcctgc aatcaatgat tataatttta
    11941 atctattatt aatt
//
LOCUS       DAVBCG010000051        11818 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBCG010000051 DAVBCG010000000
VERSION     DAVBCG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11818
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.051"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            171..2462
                     /gene="pcrA"
                     /locus_tag="VQ495_001162"
     CDS             171..2462
                     /gene="pcrA"
                     /locus_tag="VQ495_001162"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2531123.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2505..3185
                     /gene="radC"
                     /locus_tag="VQ495_001163"
     CDS             2505..3185
                     /gene="radC"
                     /locus_tag="VQ495_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2531124.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3182..3871)
                     /locus_tag="VQ495_001164"
     CDS             complement(3182..3871)
                     /locus_tag="VQ495_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2531125.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3889..4530)
                     /locus_tag="VQ495_001165"
     CDS             complement(3889..4530)
                     /locus_tag="VQ495_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2531126.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4613..4828)
                     /locus_tag="VQ495_001166"
     CDS             complement(4613..4828)
                     /locus_tag="VQ495_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2531127.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4836..5183)
                     /locus_tag="VQ495_001167"
     CDS             complement(4836..5183)
                     /locus_tag="VQ495_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2531128.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5188..6303)
                     /locus_tag="VQ495_001168"
     CDS             complement(5188..6303)
                     /locus_tag="VQ495_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2531129.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6313..7272)
                     /locus_tag="VQ495_001169"
     CDS             complement(6313..7272)
                     /locus_tag="VQ495_001169"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2531130.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7385..7954)
                     /locus_tag="VQ495_001170"
     CDS             complement(7385..7954)
                     /locus_tag="VQ495_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2531131.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8082..8753
                     /locus_tag="VQ495_001171"
     CDS             8082..8753
                     /locus_tag="VQ495_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2531132.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8737..9555
                     /locus_tag="VQ495_001172"
     CDS             8737..9555
                     /locus_tag="VQ495_001172"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2531133.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9552..10448
                     /locus_tag="VQ495_001173"
     CDS             9552..10448
                     /locus_tag="VQ495_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2531134.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10492..11466
                     /gene="pta"
                     /locus_tag="VQ495_001174"
     CDS             10492..11466
                     /gene="pta"
                     /locus_tag="VQ495_001174"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2531135.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3435 a         2265 c         2545 g         3573 t
ORIGIN      
        1 gtattgagct agtttatgaa aaaattgggt gaaaaagtcg agtgttttag aaacccacag
       61 tgtagtattc tagtttcaat ccactatatt ttgctactcc ccgtaaagtt tctattttcc
      121 ctgatttctg atataataga aatattgact tcaagagtaa ggaagagaag atgaacgcat
      181 tattaaatgg aatgaatgac cgtcaggctg aggcggtgca aacgacagaa ggtcccttgc
      241 taatcatggc aggagctggt tctggaaaga ctcgtgtttt aacccaccgt atcgcttatt
      301 tgattgatga aaagctggtc aatccttgga atatcttggc cattaccttt accaacaagg
      361 ctgcgcgtga gatgaaagag cgtgcttata gcctcaatcc agctactcag gactgtctga
      421 ttgcgacctt ccactccatg tgtgtgcgta ttttgcgtcg cgatgcggac catattggct
      481 acaatcgtaa ttttacaatt gtggatcctg gtgaacagcg aacgctcatg aaacgtattc
      541 tcaaacagtt gaacttggat cctaaaaaat ggaatgaacg aactattttg gggaccattt
      601 ccaatgctaa gaatgatttg attgatgatg ttgcttatgc tgctcaagct ggcgatatgt
      661 atacgcaaat tgtggcccag tgttatacaa cctatcaaaa agaacttcgt cagtctgaat
      721 ccgttgactt tgatgatttg attatgctga ccttgcgtct ctttgatcaa aatcctgatg
      781 ttttgaccta ctaccagcaa aaattccaat acatccacgt tgatgagtac caagatacca
      841 accacgctca gtaccaattg gtcaaactct tggcttcccg ttttaaaaat atctgtgtgg
      901 ttggggatgc ggaccagtct atctacggtt ggcgtggtgc tgatatgcag aatatcttgg
      961 actttgaaaa ggattacccc aaagctaagg ttgttttgtt ggaggaaaat taccgctcaa
     1021 ccaaaaccat tctccaagcg gccaacgagg ttattaaaaa taataaaaat cgccgtccta
     1081 aaaatctctg gactcaaaac gctgatgggg agcaaatcgt ttactatcgt gctgatgatg
     1141 agctggatga ggctgtattt gtagccagaa ccatcgatga acttagtcgc agtcaaaact
     1201 tccttcataa ggattttgca gttctctatc ggactaatgc ccagtcccgt acaattgagg
     1261 aagccctgct caagtctaac attccttata ccatggttgg cggaaccaaa ttctacagcc
     1321 gtaaggaaat tcgcgatatt attgcttatc tcaaccttat tgctaatttg agtgacaaca
     1381 ttagttttga gcgtattatc aacgagccta aacgtggaat tggtctaggt acagttgaga
     1441 aaatccgtga ttttgcaaat ttgcaaaata tgtctatgct ggatgcttct gctaatatta
     1501 tgttgtctgg tatcaagggt aaggcagccc aatctatctg ggattttgcc aatatgatgc
     1561 ttgatttgcg ggagcagcta gaccacttaa gcattacaga gttggttgag tccgtcctag
     1621 aaaaaacagg ttatgtcgat attcttaacg cccaagcgac tctagaaagc aaggcaaggg
     1681 ttgaaaatat cgaagagttt ctttctgtta cgaagaactt tgatgacacc actgatgtga
     1741 cagaagagga aactggtctg gacaaactga gtcgtttctt aaatgacttg gctttgattg
     1801 ccgacacaga ttcaggtagt caggagacat cagaagtgac cttgatgacc ctgcatgctg
     1861 ccaaaggtct cgaatttcca gttgtctttt tgattgggat ggaagaaaat gtctttccac
     1921 ttagtcgtgc gactgaagat ccagatgaat tagaagaaga gcgccgtcta gcctatgtag
     1981 gtatcacgcg tgcagagaaa attctctatc tgaccaatgc caactcacgc ttgctttttg
     2041 gtcgtaccaa ttataaccgt ccgactcgtt ttattaacga aatcagttca gacttgcttg
     2101 agtatcaagg tctggctcgt cctgcaaata caagctttaa ggcatcatat agcagtggta
     2161 gtatttcctt tggtcaaggt atgagtttgg ctcaggctct tcaagaccgt aaacgcggtg
     2221 ctgccccaaa atcgatccag tcaagcggtc ttccatttgg tcaatttaca gctggcgcaa
     2281 aatcagcatc tagcgaggca aattggtcca ttggtgacat tgctctccac aagaaatggg
     2341 gagagggaac tgttctggaa gtttcaggta gcggtgctag gcaggaattg aaaatcaatt
     2401 tcccagaagt aggtttgaaa aaacttttag ccagtgtggc tccaattgag aaaaaaatct
     2461 aattttccat ccttctcacg aataataaag tgaggaggat ttttatgtac agtatttcat
     2521 tccaagaaga ttcactatta ccaagagaaa ggctggccaa ggaaggagtt gaagcgctta
     2581 gtaaccaaga gttgctagct attttactca ggacaggaac acgtcaagct agcgtttttg
     2641 aaattgccca aaaagtcttg aacaatcttt caagcctaac ggatttgaaa aaaatgaccc
     2701 tgcaggaatt gcagagtttg tctggtattg ggcgtgttaa ggccatagaa ttacaagcta
     2761 tgattgaact ggggcatcgt attcacaaac acgagactct tgaaatggaa agtattctca
     2821 gcagtcaaaa gttggccaag aagatgcagc aggaattagg ggataaaaaa caagagcacc
     2881 tggtggcact ctatctcaat actcaaaatc aaatcatcca tcagcagacc atttttatcg
     2941 gttctgtaac tcgtagtatc gctgaaccgc gagagattct tcactatgca atcaagcata
     3001 tggcgacttc tcttatcttg gtccacaatc atccttcagg agcggtagcg cctagccaaa
     3061 atgatgatca tgtcactaaa cttgttaaag aagcctgcga attgatgggg attgttctct
     3121 tggaccattt gattgtctct cattctaatt actttagtta tcgtgaaaag acagatttaa
     3181 tctaaagttc attaacgaca tagtcaaaga gttttttatc tttgggacga ttttcaaaaa
     3241 gaagttctgg atgccattgg acaccgagaa aggcgacatc atccgtactc atgacagcct
     3301 caatgatacc atctttagga tcatgagcca caacttttaa atttggtgct aagtccttga
     3361 tgctctggtg gtggaaggag ttgatatgag agatttctcc atagatttct tggagaacgg
     3421 tatctggttc tgttaccaag cgttgagttg tgtactcaac agaagaatcc tgccaatggt
     3481 cttcgatatc ttggtacaaa gttccaccca tggcaacgtt aaagagttgg gtaccacggc
     3541 agacagagaa aatgggcttt ttctgtttaa tagcttcctt gatgagggcc agttcgaaga
     3601 tatctctttg aaggttatag tcatcgctat caatggtttt gggttcgcca taaaattttg
     3661 gatcgacatt ttgcccacct gtcaagatga gcttgtcaat caaactgata tagtggcagg
     3721 ccatttcttg atcaccaatc ggtaggatga tgggaatccc tccagcatct ttaacgcctt
     3781 caacaaagcc ttttgctgcg tagctcatca tgatgtcatc atctggatga gctttttcgt
     3841 ttcctgtaat cccaataact ggttttttca taaaatgatt ttcgctttct aatcctcttt
     3901 tcgcatgaag tagaggaggg tttggagttc acttgtcaaa tcgacatact gaacgaccac
     3961 gtcttttggt aaatgcagat ggactggtga aaaactgaga attcctttca caccagcatc
     4021 aaccaagaga ttagcaacct cttgtgactt gacgctggga acagttagga tagcagtctt
     4081 cacatcagca tccttgattt tatccttgat ctgagaaatc ccgtaaatgg gaatcccgtc
     4141 aggagtttgg gtaccgactt caggatggtc gtctaggtca aaggccatga taatcttcat
     4201 cttgttacgt tcgtggaagc ggtagtggag aagggcatgg cccatatttc caataccaac
     4261 cagcatgaca ttggtaatag agttgtcatt gagcaaatcg gcaaaaaatg tcattagttt
     4321 tttgacatca tagccaaaac cacgacgacc aagttcacca aaataggaaa aatcacgacg
     4381 tacggtcgct gaatcaatac cgatagcctc tgcaatttgc ttagagttgg cacgttcaat
     4441 cttttctgca tgaaatctct taaaaattcg atagtagaga gagagtcttt ttgctgtagc
     4501 ttttggaata gcaaactgtt tatctttcac aaaatcacaa cctttctatt cttctatttt
     4561 atagaaacat tgtgaaaaaa tcaacaaaaa taagaaaaaa ctaagaaaaa tcttagtttt
     4621 gatgtaaaaa atctgcatga gatagaaaac ggtagaggtc tccgaccaga ccctgataaa
     4681 ctttttggcc cctaaaagtc agagaagtca cataaagtgt atctggtaag gttacacatc
     4741 ctgacaaagt caacatgaga gcctcatgat cctcatactt gagagtacgc tctacatgat
     4801 agcagtcctt ataggtcagt tcaaacattt tggctctatc tttccgattt tgtaaagaca
     4861 ccacgttcta ccaagctatc catgaggaag tagaattttt cctgatgaat atggtggtct
     4921 tctgatttga aaatatcaac tagacgaagg ctaaacttgt cagtgatatt gattttagcc
     4981 cctgtaagtt ccttgttaat gatgattttg agttggaagc cttcaccgct gtttggcact
     5041 ttttccaaaa ggcgagtcag ttcatagtta ccaaccttag tttcaaaaaa ggtgttatct
     5101 ttgagggtga attttttaac agaagggcta agagtgtaat cgtaacgaca attttttaac
     5161 tgaatgattt tttcaaatgc catatggcta acctccgata atttctttta aggtttttgc
     5221 gagggtttgt aggtcttcaa cggtattttg tggcgacaaa ctgatgcgaa gggattcctt
     5281 caagcgttct gaatttgcgc catacatggc ttcaagaaca tggctggatt ggacaacgcc
     5341 tgcagtacag gctgagccag tagagattga aattccagct aaatctagcc gaaggagtaa
     5401 gaggtcattt ttctgaccag gaaatccaat attgagaaca taagggagat gatgttttcc
     5461 tctattcaag taatactgaa tgccctccag ctctgccaga aaggcggttt ctagattttg
     5521 tacatgttga aaatgttctt cttgtttttc taggtcttct tttagggctg caaccatgcc
     5581 tacgatagca ggcagatttt cagttcctgc acgttttttc tgttcctggt ctccgccatg
     5641 tagataggaa tcaaagtcca tgctagatgc gtagagaaaa ccgattccct taggaccatg
     5701 gaatttgtgg gcagaagcag tgagaaaatc aatgcccaat tcttctgaat gaattgggat
     5761 tttaccaata gcctgaactg catcaacatg ataggcagca gggtgttgct tgagtatttg
     5821 gccaatttca gcgatgggca gtaggtttcc tgtctcatta ttggcaaaca tggtagaaac
     5881 caaaatcgta tcgtcacgta aagccttttg aatttgctgg gctgtgattt cttgattttc
     5941 tggctggata atggttgctt caaacccaaa gtgttgaacc aagtaatcaa ttgtttcaag
     6001 gacagcatgg tgctcgatgg cagttgtgat gatatgtttt ccttgttctt ggtgacgaag
     6061 acagtagcca atgatggtag tattattgcc ttcagtccca ccagaagtga aaaagatatg
     6121 ttgaggtttt gtccttagta actgggctag ttcctgacgg gcttctcgca agagtttgcc
     6181 agcttgacga ccatgaccat gaatactaga aggatttccg tgggtttctt gcataacctt
     6241 ggtcatagct gaaatagcaa ctgctgacat aggagtcgtt gcagcattgt ccaaataaat
     6301 caaagaatca ccttatttct ttttattgta ggcaaagagt gggctgactg gttttctttc
     6361 gtgaatacgg acgatagcat caccaattaa ctcactagca gtgatgtagc atacattttt
     6421 aggagttttt tcttttgttg ctactgaatc agtcacaaga atttctttaa tattagtatt
     6481 gtcaagaagc tcagcagctc cctcgacgaa gagaccgtgg ctagaaacag cataaatttc
     6541 tgtagctcct tcacgttcaa cgattttaga agcttcagag aaggtacgtc ctgtatttaa
     6601 aatatcatca atcaagatag ctttcttacc ttcaacatca ccaataatat aaccttcgtt
     6661 acgagttgca tcgtcttgag ggtagtcgat aatggcgata ggagcatcaa gatattcagc
     6721 caggctacgc gcacgtttga cacctgaatt tttagggcta acgacaacaa catctgaacc
     6781 aagcaatcct ttatcgcagt aatgttttgc gaatagggga acagtgaaaa gattatccac
     6841 tggaatatca aagaaacctt gaacctgaac ggcatgcaaa tcaagagtca ggatacgatc
     6901 aactccagcc ttaaccagca tattggcaac tagttttgct gtaagtggct cacgaggaca
     6961 agcaatgcgg tcttgacgtg catagccaaa atatggaagg acaacgttga tactgtgggc
     7021 acttgcacgc acacaagcat cgaccatgat taacaattcc attaggtggt tgttgacagg
     7081 gaaacttgtt gattggatga tgtaaacatc ataaccacgg acactttctt cgatatttac
     7141 ttggatttct ccgtctgaaa attgacgtga tgatagtttt ccaagtggga caccaacagc
     7201 ttgggcaatt ttttgtgcaa tctcttggtt agagttgagt gcgaaaagtt tcatgttttt
     7261 tctatctgac attatagacc gtcctctgta aactttataa atcctagtta tatttacctt
     7321 acatatatga actgggattt gtgtattttt atcttttcta ttttaccaaa aaatggagat
     7381 tatttcagct atttttcata cttttgacaa atcgaaccaa ttttgaagga gctttttggt
     7441 aggaaatctg atttttctct aaaaattgtc ggaaatcctg tttgccttgc tcatgatttt
     7501 ccacttcaag ctccaattcg taatctgtta tatcaaagta tcggctctga tccagtgcca
     7561 tgagaccaat agctgttttc atttcatagc gaagcgttgt tagacaacca agaacctgcc
     7621 agttcttact ttggatacca tgtttcgcca attcatccag tactagtcct tgaggaagtt
     7681 cttccttact cagatagtcc tcagcatctt ttagttgcaa tttttggttg tattccatgt
     7741 ttccaacact ctgcgggact ttgagtgtca actcagccca gtcttcaaag gttcgaatgc
     7801 gcatagcgac tttcttttct cgcagttcaa aatcaggcgt gtcgatgtag taatttgttt
     7861 gaagaacagg agtgacacct gtgaactggt cttttagacg attgtattca tcttttttca
     7921 atagtgtttt caattcaatt tctaaatgtt tcatttttct tacctttttt tatcgttgaa
     7981 agcggattta tggtataata agcattgtat ttattgtata tgaatctgga gaaaaaatca
     8041 aagatatttt tgacggataa tatgagaaca agggagaata tatgacctta gaatgggaag
     8101 aatttctaga tccttacatt caagctgttg gtgagttaaa gattaaactt cgtggtattc
     8161 gtaagcaata tcgtaagcaa aataagcatt ctccaattga gtttgtgacc ggtcgagtca
     8221 agccaattga gagcatcaaa gaaaaaatgg ctcgtcgtgg cattacttat gcgaccttgg
     8281 aacacgattt gcaggatatt gctggcttac gtgtgatggt tcagtttgta gatgacgtca
     8341 aggaagtagt ggatattttg cacaagcgtc aggatatgcg aatcatacag gagcgagatt
     8401 acattactca tagaaaagca tcaggctatc gttcctatca tgtggtagta gaatatacgg
     8461 ttgataccat caatggagct aagactattt tggcagaaat tcaaattcgt actttggcca
     8521 tgaatttctg ggcaacgata gaacattctc tcaactacaa gtaccaaggg gatttcccag
     8581 atgagattaa gaagcgactg gaaattacag ctagaatcgc ccatcagttg gatgaagaaa
     8641 tgggtgaaat tcgtgatgat atccaagaag cccaggcact ttttgatcct ttgagtagaa
     8701 aattaaatga cggtgtagga aacagtgacg atacagatga agaatacagg taaacgaatt
     8761 gatctgatag ccaatagaaa accgcagagt caaagggttt tgtatgaatt gcgagatcgt
     8821 ttgaagagaa atcagtttat actcaatgat accaatccgg atattgtcat ttccattggc
     8881 ggggatggta tgctcttgtc ggcctttcat aagtacgaaa atcagcttga caaggtccgc
     8941 tttatcggtc ttcatactgg acatttgggc ttctatacag attatcgtga ttttgagttg
     9001 gacaagctag tgactaattt gcagctagat actggggcaa gggtttctta ccctgttctg
     9061 aatgtgaagg tctttcttga aaatggtgaa gttaagattt tcagagcact caacgaagcc
     9121 agcatccgca ggtctgatcg aaccatggtg gcagatattg taataaatgg tgttcccttt
     9181 gaacgttttc gtggagacgg gctaacagtt tcgacaccga ctggtagtac tgcctataac
     9241 aagtctcttg gcggtgctgt tttacaccct accattgaag ctttgcaatt aacggaaatt
     9301 gccagcctta ataatcgtgt ttatcgaaca ctgggctctt ccattattgt gcctaagaag
     9361 gataagattg aacttattcc aacaagaaac gattatcata ctatttcggt tgacaatagc
     9421 gtttattctt tccgtaatat tgagcgtatt gagtatcaaa tcgaccatca taagattcac
     9481 tttgtcgcga ctcctagcca taccagtttc tggaaccgtg ttaaggatgc ctttatcggt
     9541 gaggtggatg aatgaggttt gaatttatcg cagatgaaca tgtcaaggtt aagacctttt
     9601 taaaaaagca cgaggtttct aagggattgc tggctaagat taagtttcga ggtggagcta
     9661 ttctggtcaa taatcaaccg caaaatgcaa cgtatctatt ggacgttgga gactacgtta
     9721 ccattgacat tcccgctgag gaaggctttg aaaccttgga ggctattgag catccattag
     9781 atattctcta tgaggatgac cactttctag tcttgaataa accctatgga gtggcttcta
     9841 ttcctagtgt caatcactct aacaccattg ccaattttat caagggttac tatgtcaagc
     9901 aaaattatga aaatcagcag gttcacattg ttaccagact agatagggac acttctggct
     9961 tgatgctctt tgccaagcac ggttatgccc atgcacgatt agacaagcag ttgcagaaga
    10021 aatctatcga gaaacgctac tttgctttgg ttaagggaga tggacatttg gagccagaag
    10081 gggaaattat tgctccgatt gcgcgtgatg aagattccat tattaccaga cgagtggcta
    10141 aaggcggaaa gtatgcccat acttcataca agattgtagc ttcttatgga aatattcact
    10201 tggtctatat tcacctgcac actggtcgaa cccatcaaat ccgagtccat ttttctcata
    10261 tcggttttcc tttgctggga gatgatttgt atggtggtag tctggaagat ggtattcaac
    10321 gtcaggctct gcattgccat tacctatcct tttatcatcc atttttagag caagacttgc
    10381 agttagaaag tcccttgccg gatgatttca gtaaccttat tacccagtta tcaactaata
    10441 ctctataaaa actgtctcag agtataatta ttatcttaaa ggagaaaact catggaagtt
    10501 tttgaaagtc tcaaagccaa ccttgttggt aaaaatgctc gtatcgttct ccctgaaggg
    10561 gaagagcctc gtattcttca agcaacaaaa cgcttagtaa aagaaacaga agtgattcct
    10621 gttttgcttg gaaatcctga aaaaattaaa atttatcttg aaattgaagg aatcatggat
    10681 ggttatgagg tcatcgaccc tcaacattat cctcaatttg aagaaatggt ttctgccttg
    10741 gtggagcgtc gcaagggcaa aatgactgaa gaagatgtac gcaaggtttt ggttgaagat
    10801 gtcaactact ttggtgtgat gttggtttac ttgggcttgg ttgatggaat ggtgtcagga
    10861 gcgattcact caacagcttc aacagttcgt ccagctctac aaatcatcaa aactcgtcca
    10921 aatgtaactc gtacttcagg agccttcctc atggttcgtg gtacggaacg ttacctattt
    10981 ggagactgtg ccattaacat caatccagat gcagaagcct tggctgaaat tgccatcaac
    11041 tcagcaatca cagctaagat gtttggcatc gaacctaaaa ttgccatgtt gagctattct
    11101 actaaaggtt cagggtttgg tgaaagcgtt gataaggtcg ttgaagcaac taaaattgct
    11161 cacgacttgc gtcctgacct tgaaatcgat ggtgagttgc aatttgatgc agcctttgtt
    11221 cctgaaactg cagctctgaa agctcctgga agtacggtag ctggtcaagc aaatgtcttc
    11281 atcttcccag gtatcgaggc cggaaatatc ggttacaaga tggctgaacg cctgggtggc
    11341 tttgcggctg taggacctgt tttgcaaggt ttaaacaagc cagttaatga tctttctcgt
    11401 ggatgtaatg cagatgatgt ttacaagttg accctcatca cagcagctca agcagttcat
    11461 caatagtgaa aactataaag cgatatacta tgctatactg tagttatgaa actatgtacg
    11521 aaaagcactg ccattaattc ctgagaacta aattactgat tggtgtcaaa aaggaaaact
    11581 tccaagcgat gatatcctgt ctatacacga cctatagaaa tctgtaatat gtatgtccgt
    11641 aaaacgataa tttccctttt tgattttaaa tgagtatgaa aagagaattt tttcatgaga
    11701 aaacgcataa tattaagtgt cttgacacct gctattatgc gttttctggc tctttgtcaa
    11761 ctgtagtggg ttgaagaaaa gctaagctcg agaaaggaca aattttgtcc tttctttt
//
LOCUS       DAVBCG010000052        11672 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBCG010000052 DAVBCG010000000
VERSION     DAVBCG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.052"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            99..719
                     /locus_tag="VQ495_001175"
     CDS             99..719
                     /locus_tag="VQ495_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531136.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            805..1248
                     /gene="tsaE"
                     /locus_tag="VQ495_001176"
     CDS             805..1248
                     /gene="tsaE"
                     /locus_tag="VQ495_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2531137.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            1238..1756
                     /locus_tag="VQ495_001177"
     CDS             1238..1756
                     /locus_tag="VQ495_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531138.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            1764..2780
                     /locus_tag="VQ495_001178"
     CDS             1764..2780
                     /locus_tag="VQ495_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2531139.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            2864..4120
                     /locus_tag="VQ495_001179"
     CDS             2864..4120
                     /locus_tag="VQ495_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2531140.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            4175..5341
                     /gene="recA"
                     /locus_tag="VQ495_001180"
     CDS             4175..5341
                     /gene="recA"
                     /locus_tag="VQ495_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2531141.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            5648..7018
                     /locus_tag="VQ495_001181"
     CDS             5648..7018
                     /locus_tag="VQ495_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2531142.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            7394..8350
                     /gene="lytA"
                     /locus_tag="VQ495_001182"
     CDS             7394..8350
                     /gene="lytA"
                     /locus_tag="VQ495_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2531143.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            8715..8876
                     /locus_tag="VQ495_001183"
     CDS             8715..8876
                     /locus_tag="VQ495_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531144.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            8863..9069
                     /locus_tag="VQ495_001184"
     CDS             8863..9069
                     /locus_tag="VQ495_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531145.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            9115..9609
                     /locus_tag="VQ495_001185"
     CDS             9115..9609
                     /locus_tag="VQ495_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531146.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            9602..10027
                     /locus_tag="VQ495_001186"
     CDS             9602..10027
                     /locus_tag="VQ495_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531147.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            10435..11019
                     /locus_tag="VQ495_001187"
     CDS             10435..11019
                     /locus_tag="VQ495_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531148.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            11023..11220
                     /locus_tag="VQ495_001188"
     CDS             11023..11220
                     /locus_tag="VQ495_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531149.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            11210..>11672
                     /locus_tag="VQ495_001189"
     CDS             11210..>11672
                     /locus_tag="VQ495_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531150.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
BASE COUNT         3661 a         1854 c         2601 g         3556 t
ORIGIN      
        1 ctagtgtttg tttagcgtct taaagagaca tttgagaaga ttagaaacag ttctctcttt
       61 tctcctagta taatgattgg taggaaggga gagagaagat gaaatcaatg agaatcttat
      121 ttttgttagc tttaattcaa atcagtttga gtagctgttt cctatggaag gaatgcatct
      181 tgtcctttaa acaaagtaca gcttttttca tcgggagcat ggttttcgtt tcaggaatct
      241 gtgctggagt aaattatctt tatacccgta agcaagaagt ccatagtgtc ctagccagta
      301 agaagtcggt gaagcttttt tacagtatgt tactcttaat taatttgtta ggagctgttc
      361 ttgttttgtc agataacttg ttcatcaaaa atacgctgca gcaagaatta gttgactttt
      421 tattgccatc cttctttttc ctatttgggc tagatttgct gattttttta cccttgaaaa
      481 aatacgtgcg cgattttctt gctatgctag acagaaaaaa gacagtgttg gtgactattt
      541 tagcaacact tcttttctta agaaatccaa tgaccattgt ctcacttctg atttatattg
      601 gactgggctt gttttttgca gcctatcttg tcccaaattc ggttaagaag gaagtttcct
      661 tttatggtca tattttccga gatcttgtat tggtcattgt tacgctcatt ttcttttaga
      721 gaaagcctgt tttttatgga tgttatgatg ataagtttag taggaaaata gacacaaaag
      781 aaaagttttt ggtataataa gagtatgtac acaaaaaatg aagaagagtt gcaagcctta
      841 ggggagcgtt tgggccatct attagcaaag aatgatgttt taatcttaac tggagaactg
      901 ggtgcaggta aaacgacctt tactaaagga cttgcaaaag gattacagat ttctcaaatg
      961 attaaaagtc ccacctatac tatcgtgaga gagtatgaag gtcgacttcc actttatcac
     1021 ctagatgttt atcgtattga aggagatgct gattctatcg acttggatga gtttatcttt
     1081 ggtggcggcg tgactgttat tgagtgggga aatctcttag gagatgcctt gccagatgct
     1141 tatttagaat tggaaattct aaaagaagca gatggacgcc gtttaaattt tcaggcaaag
     1201 ggtttgcgtg ctgagaaatt gttagaggag cttcaatatg gagtatgaat tgctcattag
     1261 ggaagcagag cccaaagatg cagctgaatt agtggccttt ttaaatcgtg tgagtttgga
     1321 gacagacttt accagcctag acggagatgg tattctcttg actagtgagg agatggaaat
     1381 attcctcaac aagcaagcta gttcggacaa tcagataacc ttacttgcat tcttaaatgg
     1441 caaaattgct ggtattgtaa atattacagc tgatcagcgt aagagagtcc gtcatattgg
     1501 agatctcttc attgtgattg gaaaaagata ttggaataat ggcttgggaa gtttgttgct
     1561 agaagaagcg atagagtggg cacaagcaag tggcattctg cgtcgtctcc aactgactgt
     1621 ccaaactcgt aatcaagcag cagtccatct ttatcaaaag catggctttg tcattgaagg
     1681 tagccaagag cgtggggcat atatagaaga agggaaattt atcgatgttt acttgatggg
     1741 taaactgata ggttagtaga aatatggtta aaaaaattat tggaatggtg ctagctttac
     1801 tttctgtaac tgtagtagga gtaggtgttt ttgcttatac tatttatcaa caagggacag
     1861 aaaccttagc taaaacctat aaaaaaatcg gcgaagaaac caaggttatt gaagcgactg
     1921 aacctctaac cattctgtta atgggagtgg acaccggaaa tgttgaacga actgaaactt
     1981 gggtcggtag aagtgatagc atgatcttga tgacagtgaa tcctaaaacg aaaaaaacaa
     2041 caatgatgag tttagagcgg gatattctga cgcgcattga atcagggaat ggtcaggctc
     2101 atgaagcgaa actgaactca gcatatgcag atggtggagc agagcttgct atagaaacca
     2161 ttcaaaaaat gatgaatatc catattgatc gctatgtgat ggtcaatatg agagggttgc
     2221 aaaaactagt ggatgcagta ggaggtatta cagtcaataa tatcctaggt ttcccaattt
     2281 ctatcagtga ccaagaagaa tttaatacta tttctatcgg tgttggggag caacatattg
     2341 ggggagaaaa agccctagtc tatgcacgaa tgcgttacca agatcctgag ggggattatg
     2401 gtcgtcaaaa acgtcaacgt gaagttattc aaaaagtcat ggaaaaagct ctcagtttaa
     2461 atagcgttgg tcattatcaa gagattctaa aagctttgag tgacaatatg cagaccaata
     2521 ttgatttgtc tgcaaaaagt atccctaact tgctaggcta taaagattca tttaaaacca
     2581 ttgaaactca gcagttgcag ggtgaaggag agatacttca aggtgtttct taccagattg
     2641 tttcaagagc acatatgttg gaaatgcaaa atatactccg acgttctttg ggacaagaag
     2701 aagttactca gcttgaaacc aatgcggttt tatttgaaga tttatttggc agagcacctg
     2761 ttggtgatga agataattaa cttttgatac aaataaaaaa tcaatcgtag gaaaattcct
     2821 gcgatttttt caaaaaaata cgaatagata ggtaggagga aacatgaaag cagaaatcat
     2881 tgctgttgga acagagattt tgacaggaca gattgtcaac accaatgccc agtttttgtc
     2941 ggaaaaacta gctgagattg gggtagatgt atattttcag acggctgtag gagacaatga
     3001 agttcgtctc ttgtctttgc ttgagattgc cagtcaacgt agcagtctgg tgattttgac
     3061 aggcggtttg gggccaactg aggacgacct aaccaaacaa accctagcta aatttttagg
     3121 gaaagcatta gtctttgatc ctcaggctca ggagaagttg gatatctttt ttgccctgcg
     3181 accagactat gcccgaacac cgaataacga aagacaagct caaattgtag aaggagcgat
     3241 tccactgcca aacgaaacag gactggctgt tggaggaaaa ttagaagtag acggagtgac
     3301 ctatgtcgtc cttccaggtc cgccaagtga attgaaaccc atggtcttaa acctacttct
     3361 acccaagttg atgacaggga gcaagctgta ttcccgagtt cttcgtttct ttgggattgg
     3421 cgagagccag ctggttacga ttttggctga tttaattgat aatcaggtcg atcctacctt
     3481 ggccccttat gccaagacag gagaagtcac tctacgtctg tcaacaaagg ctagcagtca
     3541 agaagaggcg aatcaagcgc tggatatctt ggaaaatcaa atcttggact gccagacttt
     3601 cgaaggaatt tctttacgag acttttgcta tggttatggg gaagaaacta gtttagccag
     3661 cattgtggta gaagaactga aaaggcaagg gaaaaccatc gcggctgcag agagtttgac
     3721 ggcaggtctt ttccaagcta ccgtggcgaa tttttctgga atttcaagta tatttaaggg
     3781 tggttttgtg acctatagct tggaggaaaa atcaaggatg ttggatattc ctgccaagaa
     3841 tttggaagaa catggtgtgg tgtctgaatt tacagctcag aagatggctg agcaggcacg
     3901 aagcaagacc cagtctgatt ttggaattag tttgactgga gtggcaggac cagatagcct
     3961 agaaggacac ccagttggga cagtcttcat aggcttggcg caagatcaag gaactgaggt
     4021 tatcaaggtt aatattggag gcagaagccg agcagatgta cgtcacattg cggttatgca
     4081 tgcctttaac ctagttcgca aggctttatt aagtgactaa tttttgatat aatagtagat
     4141 aggtctgagg atcattagaa tgtaggagaa tagaatggcg aaaaaaccaa aaaaattaga
     4201 agaaatttca aaaaaatttg gggcagaacg tgaaaaggcc ttgaatgacg ctcttaaatt
     4261 gattgagaaa gactttggta aaggatcaat catgcgtttg ggtgaacgtg cggagcaaaa
     4321 ggtgcaagtg atgagctcag gttctttagc tcttgacatt gcccttggct caggtggtta
     4381 tcctaaggga cgtatcatcg aaatctatgg cccagagtca tctggtaaga caacggttgc
     4441 ccttcatgca gttgcacaag cgcaaaaaga aggtgggatt gctgctttta tcgatgcgga
     4501 acatgccctt gatccagctt atgctgcggc ccttggtgtc aatattgacg aattgctctt
     4561 gtctcaacca gactcaggag agcaaggtct tgagattgcg ggaaaattga ttgactcagg
     4621 tgcagttgat cttgtcgtag tcgactcagt tgctgccctt gttcctcgtg cggaaattga
     4681 tggagatatc ggagatagcc atgttggttt gcaggctcgt atgatgagcc aggccatgcg
     4741 taaacttggc gcctctatca ataaaaccaa aacaattgcc atttttatca accaattgcg
     4801 tgaaaaagtt ggagtgatgt ttggaaatcc agaaacaaca ccgggcggac gtgctttgaa
     4861 attctatgct tcagtccgct tggatgttcg tggtaataca caaattaagg gaactggtga
     4921 ccaaaaagaa accaatgtcg gtaaagaaac taagattaag gttgtaaaaa ataaggtagc
     4981 tccaccgttt aaggaagccg tagttgaaat tatgtacgga gaaggaattt ctaagactgg
     5041 tgagcttttg aagattgcaa gcgatttgga tattatcaaa aaagcagggg cttggtattc
     5101 ttacaaagat gaaaaaattg ggcaaggttc tgagaatgct aagaaatact tggcagagca
     5161 cccagaaatc tttgatgaaa ttgataagca agtccgttct aaatttggct tgattgatgg
     5221 agaagaagtt tcagaacaag atactgaaaa caaaaaagat gagccaaaga aagaagaagc
     5281 agtgaatgaa gaagttccgc ttgacttagg cgatgaactt gaaatcgaaa ttgaagaata
     5341 agctgttaaa gcagtggaga aatccgctac tttttcgatt tttgattcaa gtttttagat
     5401 tatatatagt agcttgaaat aagatatgaa caactctatt aggaaagtca aattaatttc
     5461 tagaaatgtt ttagcagcta cagcgtacta ttccaaactc aaccaactat aatagatcga
     5521 aactagaata gtacatatct acttctaaaa cattgttaaa aatcgatttg actttcctta
     5581 tttcattccg ctatatataa gtttgctgtt tcttgtcgct cctctggaaa gctgatataa
     5641 tagctttatg aataaaaaac gaacagtgga cctgatacat ggtccgattc ttccctcgct
     5701 cttaagcttc acctttccaa ttttgctatc aaatattttt caacagctct ataacactgc
     5761 tgatgtcttg attgttggac gatttcttgg tcaagaatcc ttggctgcag taggagcgac
     5821 gacagcgatt tttgacctga ttgtaggttt tacacttggt gttggcaatg gcatggggat
     5881 tgtcattgct cgttattatg gggctcggaa tttcactaaa atcaaggaag cagtagcagc
     5941 tacttggatt ttaggtgctc ttttgagcat tctagttatg ttgctgggct ttcttggctt
     6001 gtatcatctc ttgcaatact tagatactcc tgcagaaatt cttcctcaat cttatcaata
     6061 tatttctatg attgtgacct gtgtaggtgt cagctttgct tataatcttt ttgcaggctt
     6121 gttgcggtct attggtgaca gtctagcagc cctgggattt ctgattttct ctgccttggt
     6181 taatgtggtt ctggatctct attttattac gcaattgcat ctgggagttc aatccgcagg
     6241 acttgctacc attatttcgc aaggtttatc agcggttctc tgcttttatt atattcgtaa
     6301 aagtgtgcca gaactcttgc cacagtttaa acatttcaaa tgggacaaaa gcttgtacgc
     6361 ggatctcttg gagcaaggtt tggctatggg cttgatgagt tcaattgtat ctatcggcag
     6421 tgtgatttta cagttttctg ttaatacatt tggtgcagtg attattagtg cccagacggc
     6481 agctcgacgc attatgacct ttgcccttct tcctatgacc gctatttctg catcaatgac
     6541 gacctttgct tctcagaatc taggagctaa gcgacctgac cgcattgttc aaggtcttcg
     6601 aatcggcagt cgtttaagta tatcctgggc agtttttgtt tgtattttcc tcttttttgc
     6661 cagtccagcc ttggtttcct tcttggctag ttcgacagat ggttacttga tagaaaatgg
     6721 aagtctctat ctgcaaatca gttcagcctt ttatcccatt ttgagcctct tgttgattta
     6781 tcgcaattgc ttgcagggct tggggcagaa gatccttcct ctagtttcta gctttattga
     6841 actaatcgga aaaatcgttt ttgtggtttt gattattcct tgggcaggat ataagggtgt
     6901 tatcctttgt gaacctctta tctgggttgc catgacagtt caactgtact tctcactatt
     6961 ccgtcatccc ttgataaaag aaggaaaggc aattttggca accaaagtgc aatcctagtt
     7021 ggatttactg aataaaatcc atttcctcta gtgaacatcg aaaaaacttg tgttctcttc
     7081 tttagtttgg tgttgaaaat agtttaacag acttttgact tcttttatgt gatataataa
     7141 agtatagtat ttatgaaaag gacatataga gactgtaaaa atatactttt gaaaagcttt
     7201 ttagtctggg gtgttattgt agatagaatg cagaccttgt cagtcctatt tacagtgtca
     7261 aaatagtgcg ttttgaggtt ctatctacaa gcttaatcgt gactaagatt gtcttctttg
     7321 taaggtagaa ataaaggagt ttctggttct ggattgtaaa aaatgagttg ttttaattga
     7381 taaggagtag aatatggaaa ttaatgtgag taaattaaga acagatttgc ctcaagtcgg
     7441 cgtgcaacca tataggcaag tacacgcaca ctcaactggg aatccgcatt caaccgtaca
     7501 gaatgaagcg gattatcact ggcggaaaga cccagaatta ggttttttct cgcacattgt
     7561 tgggaacggt tgcatcatgc aggtaggacc tgttgataat ggtgcctggg acgttggggg
     7621 cggttggaat gctgagacct atgcagcggt tgaactgatt gaaagccatt caaccaaaga
     7681 agagttcatg acggactacc gcctttatat cgaactctta cgcaatctag cagatgaagc
     7741 aggtttgccg aaaacgcttg atacagggag tttagctgga attaaaacgc acgagtattg
     7801 cacgaataac caaccaaaca accactcaga ccacgttgac ccttatccat atcttgctaa
     7861 atggggcatt agccgtgagc agtttaagca tgatattgag aacggcttga cgattgaaac
     7921 aggctggcag aagaatgaca ctggctactg gtacgtacat tcagacggct cttatccaaa
     7981 agacaagttt gagaaaatca atggcacttg gtactacttt gacagttcag gctatatgct
     8041 tgcagaccgc tggaggaagc acacagacgg caactggtac tggttcgaca actcaggcga
     8101 aatggctaca ggctggaaga aaatcgctga taagtggtac tatttcaacg aagaaggtgc
     8161 catgaagaca ggctgggtca agtacaagga cacttggtac tacttagacg ctaaagaagg
     8221 cgccatggta tcaaatgcct ttatccagtc agcggacgga acaggctggt actacctcaa
     8281 accagacgga acactggcag acaagccaga cttcacagta gagcctgaag gcttgattac
     8341 agtaaaataa taatggaatg tctttcaaat cagaacagcg catattatta ggtcttgaaa
     8401 aagcttaata gtatgcgttt tcttgtggag atatttcctt caatttttgt tactatatta
     8461 aacaaaaatc aaaaagcaaa ctagaaagtt atgctcaaat aaaatctaaa tttgacaatg
     8521 taaaccgagt cggatagctt taagtactgt tttgaggttg aagatacgat ttttgatagg
     8581 aactcatcaa ataggtttaa tatcatcaat tttagatttt taagtagcat caataaattg
     8641 cttccttgtt ttgtcataat ttttttattt aaaaaattat gacaagaatg tgctattctt
     8701 tttatgagag gtgtatgaat atgataaatg tatgtgatgt tggaaaaaga ataaaagaac
     8761 ttagaatatc ttcaaatctt actcaagata agattgctga gtatttgtct ttgaatcaaa
     8821 gcatgattgc caaaatggaa aaaggtgaaa ggaatatcac gaatggattt aagtaataaa
     8881 gcttcaaatc ttagaaaaaa gttgggagct gatggtgaat cgccgataga tatttttaaa
     8941 ttggtacaaa agatagaaaa tttgacgctg gtattttatg gactcggaaa gaatattagc
     9001 ggagtctgtt ataaaggaac tcagttcagt ctcattgcag tcaattcaga catgccatta
     9061 ggaaggtaaa gattttcttt agcacatgga ctgtatcatc tttattatga tgaggtgaag
     9121 aagagttcag tcagtcttat cttgattggt gaaggagatg aaactgaaag aaaagcggat
     9181 cagtttgctt cttatttttt aattttccca tcttcactgt ataggatggt tgaggaaatc
     9241 agagaaaatg ccaatagaac tcatcttgaa gtagaagata ttataaaatt gggtcagttt
     9301 tatggtatca gtcataaagc tatgttatat agattgagaa atgatggata ccttgatgca
     9361 gaagaaatta aaaatatgga tattagtgtt atagagacag cttcaagatt aggctatgat
     9421 acaagtttat atcgtccttt gtcagaaagt aaaaaggaaa tggtattagg gcactatatt
     9481 aaatcgactg aacaactttt agaaaataac agaatttcgc aagggaagta tgaggaactg
     9541 ttactagatg ctttcagata tgatattgta tatgggctag atgaagaggg ggagttgtcg
     9601 tttgactagt cgtgtattta ttgatgcaga ttgtatttca gcatttttat gggttggcac
     9661 tgaacatctt ttagaaaagc tctattcggg taaaattgtt attccacaag aggtgtatga
     9721 tgaaatcaat atacctacaa ttccccattt aaaatctagg atagaccagt tggtaactaa
     9781 gggttcagct gagattgtga gcatagacat tggaactgaa gaatacgcat tatatagaga
     9841 tttaacaaga aattatgata gtaacaagat tattggtaag ggagaagcga cttctatttt
     9901 cttagcgaaa aagcataatg ggatattagg aagtaataac ctaagagatg ttaaaccata
     9961 tgtagaagaa ttttctttag aacatatgac aacaggagat atactgattg aagcgtttaa
    10021 agcgtaattt attactgaac aagagggcaa tcatatctgg aataatatgc ttaaaaagag
    10081 aaggaaaatt ggtgcaaatt cattttcaga ctatcttcgt ggaagtattc atcaaaatag
    10141 acaaaaataa atttggataa atcgaactca ctattcagga ggcatatgag caattcgaaa
    10201 aagaaaagtg tcaaattgag cctataggag tagaagtgaa atagtaagtc ctgcatagtg
    10261 gatgagagaa aagttctcct tgaagttttc ctgaactatc agtcgcatgt caaacgatat
    10321 gtagggtaat gtgagagggg atagcgagta gtttttggtt attttatcaa aaaacttata
    10381 ttttattata ccgaatgata aaatataata aaaatgatag aataaggaaa aaacatgaat
    10441 gtcaaaaaga taatgtcaat ttttcaatcc ttttatgttg atgtcagtat tgaggaactg
    10501 actttgactt taccaatcag ttttgtaaaa aggtttgagt atactcaaat gacttttcat
    10561 aaggaatcat ttttattgat taaagaaaag agaaggggga gtttgagttc atttgttact
    10621 caggctcgca ctatgggtga aaaagccaat atggatgttg ttttggtgtt ttcgaagtta
    10681 tcagacagtg aaaaaaagca attacttcaa gctagagttc cgtttgtaga ctttaaggga
    10741 aacctcttct tccctccatt gggactagta ctcaatgcga atgatactga aatccctaag
    10801 gaattaacac ctagcgaaca attaacgtgg attgcctttt tattgccaga tggcgatata
    10861 aagcagataa aatctgtttc taaccttcta tatggtggtg cttatgcttt gtcgcattca
    10921 acttttttag ctgaaacgga tgaaaatact agctatgtca tatggcagag aaaattcaat
    10981 cagttatcct tgccactttc tcagcatgtt ttaaaatgaa agatgctaga gatatggaaa
    11041 tatcgtcctt ttgtatctga gttttggaat gattttaaaa ataatcatga taaacaattt
    11101 gtagatccga tttctcttta tttgacctta aaagatgatg atgacccacg tatagaggaa
    11161 gagagtgaag cactagaaaa tatgatatta cagtatctgg gagaagatga tgccagctaa
    11221 tacgaaagtt atttttcaag aaatgtttgc ggattttcag aactattatg ttctgattgg
    11281 gggaactgct acctctatcg tattggattc gcaaggattt aaaagtcgca caacaaaaga
    11341 ttatgatatg gtcatcattt atgaagtaaa aaataaggaa ttttatacta ccttgaatca
    11401 ttttttagaa ttgggagagt atcaaggaag tcagaaagat gagaaagcgc agctttttcg
    11461 atttacaaca actaatcctg agtttccttc tatgattgaa ctatttagta tcttaccaga
    11521 atatccatta aagaaggacg gtcgagaaat tcccttacat tttgaccaag atgctagttt
    11581 atcagcctta ttattggatg aagattatta taatatattg gtgcatgaaa aagaaaccat
    11641 tcaggggtat tcggtattga gtaattgtgg tt
//
LOCUS       DAVBCG010000053        11641 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBCG010000053 DAVBCG010000000
VERSION     DAVBCG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.053"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            474..1184
                     /gene="deoD"
                     /locus_tag="VQ495_001190"
     CDS             474..1184
                     /gene="deoD"
                     /locus_tag="VQ495_001190"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2531151.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1207..1731)
                     /locus_tag="VQ495_001191"
     CDS             complement(1207..1731)
                     /locus_tag="VQ495_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2531152.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1784..2020)
                     /locus_tag="VQ495_001192"
     CDS             complement(1784..2020)
                     /locus_tag="VQ495_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2531153.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2088..3008)
                     /locus_tag="VQ495_001193"
     CDS             complement(2088..3008)
                     /locus_tag="VQ495_001193"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2531154.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3198..3602
                     /locus_tag="VQ495_001194"
     CDS             3198..3602
                     /locus_tag="VQ495_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531155.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            3818..4399
                     /locus_tag="VQ495_001195"
     CDS             3818..4399
                     /locus_tag="VQ495_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2531156.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4396..5673
                     /locus_tag="VQ495_001196"
     CDS             4396..5673
                     /locus_tag="VQ495_001196"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2531157.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5691..6353
                     /gene="deoC"
                     /locus_tag="VQ495_001197"
     CDS             5691..6353
                     /gene="deoC"
                     /locus_tag="VQ495_001197"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2531158.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6340..6729
                     /locus_tag="VQ495_001198"
     CDS             6340..6729
                     /locus_tag="VQ495_001198"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2531159.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6819..7871
                     /locus_tag="VQ495_001199"
     CDS             6819..7871
                     /locus_tag="VQ495_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2531160.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            8015..9550
                     /locus_tag="VQ495_001200"
     CDS             8015..9550
                     /locus_tag="VQ495_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531161.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9543..10601
                     /locus_tag="VQ495_001201"
     CDS             9543..10601
                     /locus_tag="VQ495_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531162.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10604..11560
                     /locus_tag="VQ495_001202"
     CDS             10604..11560
                     /locus_tag="VQ495_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531163.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         3311 a         2077 c         2667 g         3586 t
ORIGIN      
        1 taagactgac gaagtcagtc acatatataa tccaaggcga cgttgacgtg gtttgaagag
       61 attttcgaag agtataaaca gaaaggtaga gcgcgtgttc taatttgaac acgagtagaa
      121 aacttttcta aaaacaaaaa cgaaaggatg ggtaaactgt attcgctgaa ctgaatacgg
      181 gcgactctcc tctaaatcaa aattaagaaa ggaattgacc ccaccctaaa agtagtggga
      241 aaaagatagt tggtctagcg agcatcgctc actgcgccca actcctattt tcccttcgct
      301 ttttgatggg tttggtatct ttctcaatat aaaataaaga aaggtagagc gtgtgttttg
      361 atttgaacac gagcggaaaa ctcggaaaat agataatctg actgaaaaat caggatttct
      421 cgtcaggttc ctaattttca gtcgttttct tctcgctctt tgtatcataa attatgtcta
      481 tccatattgc tgctcagcag ggtgaaattg ctgataaaat tcttcttcct ggggatcctc
      541 ttcgtgctaa gtttattgcg gagaatttcc ttgatgatgc tgtttgtttt aacgaagtgc
      601 gtaacatgtt tggttacact ggtacttaca agggtcactg tgtatctgtc atgggaactg
      661 ggatgggaat gccatctatt tcgatttatg cgcgtgagtt aatcgtagac tacggtgtga
      721 agaaattgat tcgtgtggga actgcaggtt ctttgaatga agaggttcat gttcgtgaat
      781 tagttttggc gcaggcggct gcaaccaact caaacatcgt tcgtaatgac tggccacagt
      841 acgattttcc acaaattgct agctttgatt tgcttgataa agcctaccat atcgccaaaa
      901 aacttggtat gactactcac gttgggaacg ttttgtcatc tgatgtcttt tactcaaatt
      961 actttgaaaa gaatatcgag cttggtaaat ggggagtcaa ggctgtggaa atggaagcag
     1021 cagctcttta ctatcttgct gcccaatacc atgttgatgc gctagctatc atgaccatct
     1081 ctgatagctt ggtcaatcca gacgaagaca caactgcaga agaacgtcaa aataccttca
     1141 ctgatatgat gaaggttggt ttggaaacct tgattgcaga ataattatag ccaaaaagga
     1201 agccctttat gaattgtagg acttcctttt cttatccaga aattgatcta gctctctctg
     1261 atttcgaaga atagtgactt tatgtgaata ttcttggcaa agtttttggt aattttcttt
     1321 ttgagttttg ctacgcccat cccaaagaat ccatctgata aactcccact caaagcgttc
     1381 agggcaatct gccgccatac tttctctgac ttttccacgg tatttaagat aacgcttaaa
     1441 ggctctaaag agacaggtca atggcgaaaa attgagaaag atgatttggt cagcttcttg
     1501 cattcgttct tggtagtagc accaagaata attaccatcg atgacccaag ctttatgctt
     1561 ggtgagaaag ttttttatct cggttaacat ccattcgcag tcactgtctt gccaaccagg
     1621 ttgaaattgg agtgtgtcca tgtgcagttt tggaatggag tagtagttag ataacttttc
     1681 tgctatagtt gacttaccag aaccagaata tccgataatt gcgattttca ttttctactt
     1741 tttcctattt ggagacaaaa aaacagcctc tatggactgt ttcttattta gcaagtttag
     1801 ctgaaagacg agctttatcg cggcttgctt tgtttttgtg aatcaaacct ttagtttctg
     1861 ctttatcgat agctgagcta gcagcacgga aaagttcttc agatgggttt gcttcgaaag
     1921 cttttatagc agtacgcata gctgattttt gagctgagtt cttttcgttt tgtttaacgt
     1981 tcaattcagc gcgtttgata gctgatttaa tgtttgccaa tgttcttacc tccatattta
     2041 ctaactatac cattatatat gaaaacttac gttttgacaa gggaaaatta cttttttaag
     2101 taaatttcat cgatttcatg gttcttgctt ttatgaagaa tcacttctgc acgatttctg
     2161 gttggttcaa tataattttg cagatttgtg agattgatac tgatccagac ctgatgggca
     2221 aaggactcca cttccccaat cggcatctga gtaaaacgat aatagtagct atcagggtcg
     2281 ttttgggcta ggctcagcat tttcaagaaa cggtccagat accaactttc aatatcatcc
     2341 actccagcat ctacatagat ggaaaagtca aagaagtcag tgatatagag acgatcgttt
     2401 tgtggatttt gaaagacatt aattccctca acaattacaa aatcagcagc tttgacactt
     2461 tgtttctttt cgggtacgat gtcgtaaact tcatgagaat agacaggaat atctacatct
     2521 tgtccatttt tgatgcggtc caagaagttg agaagagctt ccatatcata gctttcagga
     2581 aatcctttac gatttaaaat cccctgctca atcaaggttt gattgggata gagaaaacca
     2641 tcagttgtaa ccaactcaac cgtagcatct gtaaacgtac gggacagtag gatttgaagt
     2701 aggcgactgg ttgtggattt tccaacggca acactcccag aaaccccaat aataaaaggt
     2761 tgagatttac tttcacgttg gaggaaaatt ccttttgaaa aggctaaatc ttccttagtt
     2821 cgcttgtaaa tctgaatcag atgagccaag gggagataga tatctgtaac gtcttggaga
     2881 ctgatttggt cattaaaact cttgatagat tccaattctt cttctgtcaa aggaggtgtt
     2941 gtctttcgat gtaaagattg ccaagtctgg cggctgattt tttcaaaatg taaaaattcg
     3001 ttggtcattt gttttcccct agatattttg tctatcatac cataaaaagc taaaaaaata
     3061 aagcggtttc ttgaatgtgg taagtcaatg gtatatagta taagtgatca atttattgat
     3121 gaaataggag atagattcta ctgatttata agcttataaa gggggctttt tagttcacaa
     3181 cttataggag gtgtactatg aaaatcttaa aacgttacat attggaactc tgttttattt
     3241 taagttttgc tttacctttt ataaaaggaa ccaatgcaga taatggtaga tgctttgtgg
     3301 aaacctatta cggttttact tttttgatgg aacatgctat tgtaacagct gtctttatct
     3361 attcgttttt aattgctttc ttactaaaaa aacgatgggc gaaatggatt gctgctggta
     3421 gttattgctt tttagtccta tggattgcga cagagggtta tttcttccgc atgtcactag
     3481 aagatttgat acgactttgg acaagtttgg aaatcctgac acaaacgtat cagttgggct
     3541 tttatctaaa cattttgtta ggaattttgt tgataataaa gtattttaag gttaagcaat
     3601 agaaataata atctgcctga ttatagatga taaacttttt gtttttatct ggactgtgat
     3661 atttatagtt ctaaatggtt tattatttat aagtgttgaa agagattgaa gaaaatggct
     3721 tcgtgacttg ctttcttcgg tctttttcgt tttattatca atttgtaaac gatttacaat
     3781 ttgagtcaaa ttatggtaaa atagtgtcat gagtaaaatg tattatgcag aaaatcctga
     3841 cgctgctcac gacattcatg agttgagagt ggacttgttg ggacaaaaaa tgactttttt
     3901 gacagatgcg ggtgttttta gcaagaaaat ggttgacttt ggaagtcaac tcttgctcaa
     3961 gtgtctggat gtcaaccaag gagagacagt cctagatgta ggctgtggtt atggtccatt
     4021 aggtttgtcc ttggtcaagg tttacggagt tcaggcgacc atggttgata ttaacactcg
     4081 tgccttggat ttagctcgga gaaatgctga aaaaaataat gcaaaagcga cgattttcca
     4141 gtccaacatc tatgaacaag ttgaaggaca ttttgatcat gtcatttcca atcctcctat
     4201 ccgtgcgggc aagcaagtcg ttcatgaaat cattgagaaa agtaaagatt tcttggaaac
     4261 tggtggggat ttgacaatcg tcatccagaa aaaacaaggg gctccaagtg ctaagtccaa
     4321 gatggaggat gtgtttggta attgtgaaat ccttaaaaaa gacaaaggat actatatcct
     4381 tagaagtgtg aaagaatgag agcagttgat ttaatacaaa aaaaacgaga cggtcaagaa
     4441 ctgacttcaa gtgaaattga atggctggta gaaggctatg tgtcaggaac tgttcctgat
     4501 tatcagatgt ctgcctttgc tatggctgtt tattttaaag gaatgacgac tcgtgaaatt
     4561 tctgatttaa cgatgaatat ggttaagact gggcaagagt ttgacctgtc agccattgat
     4621 ggtgtgaaag ttgacaagca ttctactggt ggtgtcggcg ataaggtaac cttgatttta
     4681 gctcctcttg ttgctagctt cggtgtgcct gtagcaaaaa tgagtggtcg tggtcttggc
     4741 catacaggcg gaacaattga taaattggag tccattaggg gctatcaagt agaacgtagt
     4801 caagaggatt ttattcgtca ggttcaggat attggagtat ctgtcattgg gcaatcagac
     4861 cagctggtca aagcagataa gcttctctat gcccttcgtg atgtgaccgc aactgtcgac
     4921 acgattcctt tgattgcgag ttcggtgatg agcaagaaaa ttgcggcagg ggcggatgct
     4981 attttgctag acgtgactgt cggtgagggt gccttcatga agacggttga tgaggcgcgt
     5041 gagttggctc aaactatggt agatcttggt aaggttgttg gacggaagac agtagcagtg
     5101 attacggata tgagccagcc tttgggacga gcgattggaa atcgtctgga aatacttgaa
     5161 gcattggaga ttttgcaagg tcaaggacgt caggatatta cccactttat ttgtgaattg
     5221 gctcaaatta tgcttggttt ggcaaatgtt aacaagactg ttgaagaagt tcaccaacat
     5281 cttgagaatg gtcaagcact ggctaagttt gaggaaatgg tgcaagccca aggtggagac
     5341 ttggaagacc tctatcgtcc tgtaaatgta gctcatgtgg tggaaatccc tgctcaggaa
     5401 acgggtgtca tttcagctct tccagctatg gactttggtc tttatgccat gaggctgggg
     5461 gctggtcgtg cagttaagtc tgatgccttg gactatgaaa caggaattgt ttttgaaaag
     5521 aagattggag actccgttca aaagggtgaa attgttgcaa aagtatacac aaatgaaaaa
     5581 attccacctc aactagttac agatttccaa aaatgtgtta aaataagtga tgaagtgaaa
     5641 aaaatccgtg aaattgtaga aatcatctcc taaatggagg gaaaaccgaa atgaaattaa
     5701 ataaatatat agatcatacg cttttaaaac aagatgcaaa gaaaaaacaa attgatagtt
     5761 tgttgtctga ggctagagag tatgactttg ccagtgtttg cgttaatccg acctgggttg
     5821 aacatgctaa aaaaggactt gaaggcacag atgttaaggt ttgcacggta gtaggtttcc
     5881 ctttgggagc aacaacttca gccgtgaaag catttgagac aaaagaagct atccaaaatg
     5941 gtgcagatga gattgatatg gtgatcaatg ttggagctct caaatcaggt aatttagcct
     6001 tggttgagtc agatattcgc gcagtagtgg aagcaagtgg tgataagtta gtgaaagtca
     6061 ttattgaagc ttgccttctg acagaccaag aaaaaattgt tgtttgccaa ttggcccaaa
     6121 aagctggggc tgactttgtt aaaacatcta ctggtttttc aactggtggt gctacgatag
     6181 cagatgttac attaatgcgt gaaacagttg gatctgatat gggagtcaag gctgctggtg
     6241 gagctcgttc ttatgcagat gctcttgcct ttgtcgaagc aggtgcgacc cgtatcggaa
     6301 cgtcagctgg ggtagctatt ttaaaaggag aattggcaga tggcgactac tgagttgatt
     6361 gaactggcaa ttgaaaccag caaacatgcc tatgtcccct attctcactt tcctatcgga
     6421 gcagttttag tagccaaaga cggaagtgtt tacacgggag tgaatatcga aaatgctagc
     6481 tatcctttga ccaattgcgg tgagagaaca gctattttta aggctatatc tgaaggccaa
     6541 agagaatttt cagaattgat tgtctatggt caaactgaaa aaccgatctc gccatgtggt
     6601 gcttgtcgcc aagtgatggc tgaatttttt gaacaagatc taaaagtgac cttagtcgca
     6661 aaagataaat cgacggtcga gatgacggtc ggggaattac ttccatactc ttttacagac
     6721 ttaaactagt ctgagtcgct ctctgagtgg cacggtcctt gtggccaacc aatccatact
     6781 tgcaacatcg ttgcacatct tatttaggag gttcagtaat gaacaagaaa caatggctag
     6841 gtcttggcct agttgcagtg gcagcagttg gacttgctgc atgtggtaac cgctcttctc
     6901 gtaacgcagc ttcatcttct gatgtgaaga caaaagcagc aatcgtcact gatactggtg
     6961 gtgttgatga caaatcattc aaccaatcag cttgggaagg tttgcaggct tggggtaaag
     7021 aacacaatct ttcaaaagat aacggtttca cttacttcca atcaacaagt gaagctgact
     7081 acgctaacaa cttgcaacaa gcggctggaa gttacaacct aatcttcggt gttggttttg
     7141 cccttaataa tgcagttaaa gatgcagcaa aagaacacac tgacttgaac tatgtcttga
     7201 ttgatgatgt gattaaagac caaaagaatg ttgcgagcgt aactttcgct gataatgagt
     7261 caggttacct tgcaggtgtg gctgcagcaa aaacaactaa gacaaaacaa gttggttttg
     7321 taggtggtat cgaatctgaa gttatctctc gttttgaagc aggattcaag gctggtgttg
     7381 cgtcagtaga cccatctatc aaagtccaag ttgactacgc tggttcattt ggtgatgcgg
     7441 ctaaaggtaa aacaattgca gccgcacaat acgcagccgg tgcagatatt gtttaccaag
     7501 tagctggtgg tacaggtgca ggtgtctttg cagaggcaaa atctctcaac gaaagccgtc
     7561 ctgaaaatga aaaagtttgg gttatcggtg ttgatcgtga ccaagaagca gaaggtaaat
     7621 acacttctaa agatggcaaa gaatcaaact ttgttcttgt atctactttg aaacaagttg
     7681 gtacaactgt aaaagatatt tctaacaagg cagaaaaagg agaattccct ggcggtcaag
     7741 tgatcgttta ctcattgaag gataaagggg ttgacttggc agtaacaaac ctttcagaag
     7801 aaggtaaaaa agctgtcgaa gatgcaaaag ctaaaatcct tgatggaagc gtaaaagttc
     7861 ctgaaaaata atggatggaa gtcatttctt aggaagcggc caagggccgc ttttttaaga
     7921 gttgagacaa agtcattttg tctcagtaaa gcttgctact agacaaacta gcaagtttta
     7981 ttgaaataaa ataagactct gaaaggaaga gcacatggca cacgaaaatg tcattgagat
     8041 gcgtgatatt accaaggtgt ttggtggatt tgttgccaac gacaaaatca acttgcacct
     8101 acgaaaaggt gaaattcatg cacttttagg agaaaatggg gctggtaagt ccacgctaat
     8161 gaacatgtta gcaggccttc ttgaaccaac tagtggtgaa atcgcggtca acggtcaagt
     8221 tgtcaatctc gactccccat ctaaagcagc tagcttggga atcgggatgg ttcaccagca
     8281 ctttatgttg gttgaagcct tcacagtggc tgaaaacatc attttaggta gtgaattgac
     8341 taaaaatggt gtgctagata tcgctggagc tagcaaagaa atcaaggctc tttctgaacg
     8401 ttatggctta gctgttgacc cttctgccaa ggtagcagat atctcagttg gagcccaaca
     8461 acgtgtagaa attttaaaaa cactttatcg gggggctgat atccttatct ttgacgaacc
     8521 aacggctgtt ttgactccat cagaaattga tgagttgatg gctattatga aaaatcttgt
     8581 caaagaagga aaatcaatta tcttgattac ccacaaattg gatgaaattc gcgcagtttc
     8641 tgaccgtgtt acagttatcc gtcgtgggaa atcaattgaa accgttgaaa ttgcaggggc
     8701 taccaatgct gatttggcgg aaatgatggt aggacgttct gtttccttta aaacagagaa
     8761 gcaagcctct aaaccaaaag aagtggtttt gtctatcaaa gatttggtgg tcaatgaaaa
     8821 ccgtggtgtt ccagctgtta aaaatctgtc cttggatgtt cgtgctggag agattgttgg
     8881 tattgcgggg attgatggaa atggtcagtc tgaactgatt caagccatta caggtcttcg
     8941 taaggttgaa tctggtagca ttgagctaaa aggagattca attgtaggct tgcacccacg
     9001 tcagattaca gaactaagtg ttgggcacgt tccagaagac cgtcaccgtg atggcttgat
     9061 tttggaaatg atgatatctg aaaatattgc ccttcaaacc tactataaag aaccacatag
     9121 taaaaatgga attttgaatt attcaaatat tacttcttat gctaaaaagc tgatggaaga
     9181 gtttgatgtt cgcgctgcca gtgaattagt tcctgcagct gcactctcag gaggaaatca
     9241 acaaaaagca attattgctc gtgaaattga tcgagatcct gatctcctta tcgttagcca
     9301 gccaactcgt gggttggatg tcggtgccat tgagtatatc cacaaacgct tgattgaaga
     9361 gcgtgataat ggcaaggctg tccttgttgt cagctttgaa ttggatgaga ttttaaacgt
     9421 ctcagaccgt attgccgtta tccacgatgg taagattcaa ggtattgtat caccagaaac
     9481 aaccaataaa caagaacttg gtgtcttgat ggctggtgga aacttgggaa aggagaagag
     9541 tgatgtctaa aaaattacaa caaatttcgg ttcccttgat ttctgtattt ctaggaattt
     9601 tactcggagc cattgtcatg tggatcttcg gttatgatgc tatttggggc tacgaagaat
     9661 tgttctatac agcctttggc agtctgcgtg ggattggaga aatcttccgt gctatgggtc
     9721 ctctggtctt gattggtctt ggttttgcgg ttgccagtcg agctggtttc tttaacgtcg
     9781 gacttcctgg tcaggctttg gcaggttgga ttctcagtgg ttggtttgcg ctgtcgcatc
     9841 cagatatgcc ccgtcccttg atgattctag caaccatcgt gattgccttg attgctggtg
     9901 ggattgtcgg agcgattcca ggtattctta gggcctatct agggacgtca gaggttattg
     9961 taaccatcat gatgaactac attgtcttgt atgtagggaa tgcctttatc catgctttcc
    10021 ctaaagactt catgcaaagt acagattcga ccattcgtgt tggggctaat gcaacctatc
    10081 agacaccttg gttggctgag ttgactggta actcacggat gaatattggt attttctttg
    10141 ccatcattgc cgttgcagtt atttggttca tgctcaagaa aacaactctt ggttttgaaa
    10201 tccgtgcagt tggtcttaat ccacatgctt cagaatatgc tggtatttct gccaagcgga
    10261 ctattatcct atctatgatt atttcaggtg ccttggcagg tcttggtgga gctgttgaag
    10321 gtttgggaac cttccagaac gtctatgttc aaggttcgtc attagctatc ggatttaacg
    10381 gaatggcggt tagtttgctt gcggccaact caccaattgg tatactcttt gcagccttcc
    10441 tatttggcgt tctccaagtt ggggctcctg gtatgaatgc ggcgcaggta ccatctgagc
    10501 ttgtcagcat tgtaacagcg tctattatct tctttgtcag tgttcattac cttatcgaac
    10561 gctttgtcaa accgaaaaaa caagttaaag gaggtaagta aagatgtcta ttataacctt
    10621 gctccccctc ttggtgtctt ctatgctgat ttactcagca cctctcatct ttacaagtat
    10681 cggtggtgtt ttctctgaac gtggtggtgt ggtaaacgtt ggccttgaag gaattatggt
    10741 tatgggtgcc ttttctggag ttgtctttaa ccttgaattt gcagaacaat ttggagcagc
    10801 aactccatgg ctatccttgc ttgtagcagg attggttggt agtgttttct ctatcatcca
    10861 cgcagcagcg acggttcatt tccgtgcaga ccatgttgtc agcggtacgg tattgaactt
    10921 gatggcgcct gccttggctg ttttcttagt taaagttctt tataacaaag gacaaaccga
    10981 caacctaagt caaacttttg gacgctttga tttcccagtc ttggcaaata tcccagtgat
    11041 tggtgatatc ttcttcaagt caactagtct acttggttat ctggcgattg ccttctcatt
    11101 ccttgcttgg tttattctct ttaagactca atttggtctt cgtctccgct ctgtcggtga
    11161 acatcctcaa gcagcggata ccttgggaat caacgtctac aagatgagat atttagggat
    11221 tattatttca ggttttctag gtggaattgg cggagcgatt tatgcgcaat caatctcagt
    11281 taacttctca gtgacaacta ttgttggacc tggatttatc gcccttgctg cgatgatctt
    11341 tgggaaatgg aatccaatcg gtgctatgct ttctagtctc ttctttggac tttcacaaag
    11401 tttggctgtt atcggttctc aattgccgtt cctacaagga gtgccagcgg tttatcttca
    11461 aattgcacct tatgttttga caattcttgt cttagcagcc ttctttggaa aagcagtcgc
    11521 accaaaagca gatggtatca actacatcaa atcaaaataa gcatacaaaa aaacgtcagt
    11581 ccaatgactg aactgccccc caaaagttag acagaaaaaa tctaactttt ggggtgtttt
    11641 t
//
LOCUS       DAVBCG010000054        11504 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBCG010000054 DAVBCG010000000
VERSION     DAVBCG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.054"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            131..385
                     /gene="blpM"
                     /locus_tag="VQ495_001203"
     CDS             131..385
                     /gene="blpM"
                     /locus_tag="VQ495_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2531164.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            401..604
                     /gene="blpN"
                     /locus_tag="VQ495_001204"
     CDS             401..604
                     /gene="blpN"
                     /locus_tag="VQ495_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2531165.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            848..997
                     /gene="blpO"
                     /locus_tag="VQ495_001205"
     CDS             848..997
                     /gene="blpO"
                     /locus_tag="VQ495_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2531166.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            1101..1220
                     /locus_tag="VQ495_001206"
     CDS             1101..1220
                     /locus_tag="VQ495_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2531167.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            2006..2404
                     /locus_tag="VQ495_001207"
     CDS             2006..2404
                     /locus_tag="VQ495_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2531168.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            2442..3131
                     /locus_tag="VQ495_001208"
     CDS             2442..3131
                     /locus_tag="VQ495_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2531169.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3173..3421
                     /locus_tag="VQ495_001209"
     CDS             3173..3421
                     /locus_tag="VQ495_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531170.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            3451..4062
                     /locus_tag="VQ495_001210"
     CDS             3451..4062
                     /locus_tag="VQ495_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2531171.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4223..5017
                     /locus_tag="VQ495_001211"
     CDS             4223..5017
                     /locus_tag="VQ495_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2531172.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            5014..5649
                     /gene="trmB"
                     /locus_tag="VQ495_001212"
     CDS             5014..5649
                     /gene="trmB"
                     /locus_tag="VQ495_001212"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2531173.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5775..6254
                     /gene="rimP"
                     /locus_tag="VQ495_001213"
     CDS             5775..6254
                     /gene="rimP"
                     /locus_tag="VQ495_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2531174.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6298..7434
                     /gene="nusA"
                     /locus_tag="VQ495_001214"
     CDS             6298..7434
                     /gene="nusA"
                     /locus_tag="VQ495_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2531175.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7456..7749
                     /locus_tag="VQ495_001215"
     CDS             7456..7749
                     /locus_tag="VQ495_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2531176.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7742..8041
                     /locus_tag="VQ495_001216"
     CDS             7742..8041
                     /locus_tag="VQ495_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2531177.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8058..10850
                     /gene="infB"
                     /locus_tag="VQ495_001217"
     CDS             8058..10850
                     /gene="infB"
                     /locus_tag="VQ495_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2531178.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11101..11451
                     /gene="rbfA"
                     /locus_tag="VQ495_001218"
     CDS             11101..11451
                     /gene="rbfA"
                     /locus_tag="VQ495_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2531179.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3512 a         2015 c         2619 g         3358 t
ORIGIN      
        1 taatttttgc aattcaagac gtttcgatga ctattcaaga tctggatgaa aaattttaaa
       61 aaacgatgat atactaaact tgtcaaagtt acaacaagac aaaaattaaa aataaaaaag
      121 agtatttgtc atggatacaa aaattatgga acaatttcat gaaatggata taactatgtt
      181 atctagtatt gaaggaggca agaataattg gcaaactaat gtcttggaag gtggtggtgc
      241 tgcttttggt ggctgggggc tagggacagc tatttgtgct gcgagtggtg ttggagcacc
      301 atttatggga gcatgtggat acataggagc taaatttggt gtggatcttt gggcaggagt
      361 aacaggggca acgggtggat tttaataaaa ggagacttgt atgaatactt attgtaatat
      421 aaatgaaaca atgctgagtg aggtttatgg aggtaattcc ggaggagcag ctgtagttgc
      481 tgctttaggt tgtgcagcgg gtggagtgaa atatgggaga cttctaggac catggggcgc
      541 tgcaatagga ggaattggag gagcagtggt ttgtggatat ttagcctata ccgctacatc
      601 ataaaaatga aagctaaatt ttatgaaggc tatattcttt attattctct tcgcttttca
      661 aacctatctt atttatctat ccataagtat ctccgataaa aaacaaaaga ctattgaatt
      721 aacatcattg aattgttttg tcatattatt tctaatctat gataaattaa tttttctctt
      781 tattgcctat gtttttttga taatttttat attaaacttg ttccgtaact gaaaagtgat
      841 atactatatg gatacaaaaa tgatgtcaca atttgcagtt atggataatg aaatgcttgc
      901 ttgcgttgaa ggtggagata ttgattgggg aagaaaaatt agttgtgcag caggggttgc
      961 atatggcgca attgatgggt gtgcaacaac ggtttgacat ttctattggg accatttgct
     1021 ataggaatag gtgtaactgg tgctgcaggt ggagctatac ttggaggtgt agcctatgca
     1081 gcaatatgtt ggtggtaatt atggatttta aaagttttat tattggttta gtagttggta
     1141 tatttggtcc ttatatggat gacttaatta gaaaaatatt ttcaaaatct tccaaaaagg
     1201 atacggatag tactctctaa aaaataaatt tctcccctgt atttgctagc ttaatttaca
     1261 ggggagtttc tttaatattg tttcgcctgt caaaatgata tgtgatagca ggatgaaaag
     1321 tctacaaaca agttggtatc aatacaatat aaagaggaaa gtatgttttt aatgcttact
     1381 ttttaaattt tttaacaatt caagatgttt cgatgacaat tcaagatttg gatgaaaaat
     1441 tttaaaaaac aatgatatac taaacttgtc aaagttgcaa cgggtgctgc tacattatgt
     1501 taaaaatcgc tcttgattga taagctggat ttgttaggaa aggagtaaat aattatggat
     1561 aagaaaaaga tagtttcgac tataatatgt atagtatttc ttgtagtgtc agtagataat
     1621 ttttttagag acttaacccc gttactgttc attttaaata ttattggcct gtcttgcttt
     1681 tcagtgctaa catacattaa cataaaagag atactcttaa atataagtaa atgatgattt
     1741 ggttagtcct agaaccaatc aacttatgtt tatcggcttg gcagatttta tgtttgtcat
     1801 ttgtttgtat agaggtattt cagaaacaga attctatcaa caactaatcg cttatatagg
     1861 tgtattctct gcttgtttat cacgcttctg ctcatgtggg gcttgaagta ttataaaaag
     1921 tagctttcta tccttttctg acaggatatt tgagtagaag gctctattaa attgttagtg
     1981 aaataaatga atggaggtat ttaatatgaa atacagatta ttttttgtta ttttcttgag
     2041 tagtgtgttg gatattcttt tagggacatt tttacaaatc tctatcgtat ctattgggtg
     2101 gcttgttctt tacagtggtt tgtttgaagt aggagttttc cttcttgcta ataaaggggt
     2161 ggcggtaaaa atcaaggaag tagatattcg aaatcgcttt aaatttattt ttggaaaaac
     2221 cttatggttt caaattcttt tgctcatctt tttgataatc aaactttatc ttggtttgga
     2281 tgcgaggttg attttattct atggacatat tttcattgtc tttaatgcct taatgtatct
     2341 attatctagt agtcaggtta gccttaaaaa aaaacaaact gtcttcttaa tcttacagca
     2401 gtagagtctg acaaggtgag gtcatagtag ggaggatttc tatgaaaaag tatcaacttc
     2461 tattcaaaat aagtgcagtc ttctcttact tatttttcgt atttggtctt tctcagctga
     2521 cgcttatcgt ccaaaattat tggcaatttt cttcccagat tggtaatttc gtctggattc
     2581 aaaatatctt gagtttactt tttattggag tcatgattgt ggttcttgtt aagacaggcc
     2641 atggttatct cttccgcatt ccaagaaaaa aatggctttg gtattcgatt ttgacagtat
     2701 tagtggtagt gttccagatc tcttttaacg ttcagacagc taaacatgtt cagtcaactg
     2761 cggaaggttg ggctgtattg attggttata gtgggactaa ctttgcagag ctaggtattt
     2821 atatagccct gttctttctg gttccactga tggaagaatt gatttataga ggattactgc
     2881 aacatgcttt ctttaagcat tcgcgatttg gtcttgattt gcttcttcct tctattttat
     2941 ttgctctccc tcatttttca agcctgccta gtctgttaga tatcttcgtc tttgcaacag
     3001 ttggaatcat ctttgctggt ttgacccgct ataccaagag catttatcca tcctatgcgg
     3061 tgcatgtgat caataatatt gtagcgacct tcccgttttt gctcactttt ctacataggg
     3121 tcttggggta aaaaaacaaa aggcttgctt ttcagccata gaggaggtca tcatgtataa
     3181 acacttattt ttcctagatt ccaaaacttt agatcggttg acaccctata ttctagtctt
     3241 ggcttctgac accattgctt ttaatgtttt tgtgctaacc tttgtatctg cggtggtttt
     3301 taatttccta aattccatgc tagctttaat ggctatattc ataggggctg gctatgtggt
     3361 cggattttgg ttgctaaaat ggtttgtttt ggaaaggtta gagctaaagg atgacgcgta
     3421 ggaagtctgt ttggtgagga ggatagtttt atggagtttt ttgataaatt tcatgccttg
     3481 tgttttggat ttttagtact aataattgtc attacagttc cttatacgat taaccatggg
     3541 ggtttttttc aaaatgaatc tgcattgatt cttgtaagtc ttcttgtaac ctcgctgagt
     3601 gttgcttatg ctagaaagtt tgaaatgatt tcttttggga tgttaagcaa gaaacaactt
     3661 ttgcttttca ttgcaatctt tcttctaagt gtacttgaga cgctggttta tattcatttc
     3721 ttcgctgttt cttctggctc aggggtccaa cacttggcgg aagtcagcag aggaatttcc
     3781 ctgtctttga ttttgactac ctcagttttt ggccccatcc aggaggaact cattttcaga
     3841 ggacttcttc aaggtgcggt ttttgacaat tcttggttag ggcttgtgct aacttcctct
     3901 ctcttttctt tcatgcatgg accttctaat gtcccttcgt ttatttttta tctacttggg
     3961 ggtttgttgc tgggctttgc ttataaaaag agtcaaaacc tatgggtttc tactctagta
     4021 cacatgtttt acaacagttg gccactccta tattatttat aaaaattatg aaaaggtaag
     4081 cagaagacgg tgcttacctt tttcaagcta taatgatacc caagccaatc cagaggcttt
     4141 tctttgagaa tcgggttaga gtggttgacg aataggccaa aaactagtag aatagtaagg
     4201 aaactttata cggaggaaag aaatggattt gggtgataat gagctaacac tgactcccat
     4261 acctgggaaa agtggcaagg cttatatggg tagctatcct gacgggaagc gcatctttgt
     4321 aaaaatgaac acctctccaa tcctacctgg tctagctaga gaacaaattg ctccacaatt
     4381 attatggagt cgtcgtttgg cagatgggcg tgatatgtgt gctcaagaat ggttgacagg
     4441 caagatattg accccctatg atatgaatcg taagcaaatc gtcaatattt taacccgtct
     4501 tcatcgctca cgtccgttga tgacacaatt gagtcgtttg ggctatgcca tggaaacacc
     4561 tgtagattta ctacagtctt ggcaggaaac ggctccagat gctttgcgta aaaatcattt
     4621 tatcagtgaa gtgatggctg atttacgtca gactattcca ggatttagag aggaccatgc
     4681 gaccattgtc catggagatg tacgacatag taattggatt gagacagata gtggcttgat
     4741 ttatttagta gattgggatt cggttcgctt gaccgatcgc atgtttgatg tggcccatat
     4801 gctctgccat tatatttcag aacatcagtg gaaggaatgg ttgacctact acggttacaa
     4861 gtacaatcaa acggtattaa gtaaattgta ttggtatggt caattgtctt atttgagtca
     4921 gatttccaag tattatatga accaagattt agaaaatgtc aatcgggaga ttcatggttt
     4981 gcgtcatttc cgagacaagt atggaaagag aagatgagag ttagaaatcg taaaggggca
     5041 acagaattac tagaggcaaa tccccagtat gtggtcctca atcccttgga agccaaggca
     5101 aaatggcggg acttgtttgg caatgataat cccattcatg tggaagttgg aagtggaaag
     5161 ggtgcctttg tttcaggtat ggccaagcaa aaccctgaca tcaactatat cgggattgat
     5221 attcaaaagt ctgttttgag ctacgctttg gacaaggtgc ttgaagttgg agtgcctaac
     5281 atcaagctct tgtgggtaga tggttctgac ttaactgact attttgaaga cggtgagatt
     5341 gatcgcttgt atctgaactt ttcagatcca tggccgaaaa aacgccatga aaagcgtcgt
     5401 ttgacctaca agaccttctt ggataccttc aaacgtatct tgcctgaaaa tggagaaatt
     5461 catttcaaga cggataaccg tggcttgttt gagtacagtt tagtgagctt ttctcaatat
     5521 ggcatgaaac tcaatggtgt ctggttagat ttgcatgcca gtgattttga aggcaatgtc
     5581 atgacagaat acgagcaaaa attctcaaac aaggggcaag ttatctaccg agttgaggca
     5641 gaattttaag agatagccta aaattaggct gtacaagtgc ttttgcttta cataagttgg
     5701 caaacgtgct atactgatag taagaatatg aaaagtgagg cggggaaata tcttcgcctc
     5761 ttgcttatga ggaggtggac gcaatcgcaa caatcgtaga attagtcaga gaagttgtag
     5821 aacctgtcat agaagctcct tttgaactcg tggatatcga gtatggaaag attggcagtg
     5881 acatgattct cagtattttt gtagataaac ccgaaggaat taccttgaac gacacggcag
     5941 acttgacaga aattatcagt cctgtcctag acaccatcaa gccagatccc ttcccagaac
     6001 aatatttcct agaaattacc agtccaggtt tggaacgccc tttgaaaacc aaggatgccg
     6061 tcgctggagc ggttggaaaa tacatccatg tcgggctcta ccaagccatc gataagcaaa
     6121 aggtctttga aggaaccttg ttggccttcg aagaggacga gttgactatg gaatatatgg
     6181 acaagacgcg taagaaaacc gtccaaattc catacagttt agtatcaaaa gcacgtttag
     6241 cagttaaatt atagaaaaag aaaggatagc ttttgaggat tcaaaagtga agaaaacatg
     6301 agtaaagaaa tgctagaggc cttccgcatt ttggaagaag acaagggaat caaaaaagaa
     6361 gatatcatcg acgcagtagt agagtcgctt cgttccgctt atcgcagacg ctatggtcag
     6421 tcagacagcg tagctattga cttcaacgaa aaaacaggtg actttacagt ttatactgtc
     6481 cgtgaagttg ttgatgaagt atttgatagc cgtttggaaa tcagcttgaa agatgctctt
     6541 gccattaatt cagcttatga acttggagac aaaatcaagt ttgaagaagc accagctgag
     6601 tttggtcgtg tagcagccca gtctgccaaa caaaccatca tggaaaaaat gcgcaagcaa
     6661 acacgtgcca tcacttacaa tacttacaaa gaacatgagc aagaaatcat gtctggtaca
     6721 gtagaacgct ttgacaaccg ctttatctat gtcaaccttg gtagcatcga agcccaattg
     6781 tcaaaacaag accaaattcc tggagaagtt tttgcttctc atgatcgtat cgaagtttat
     6841 gtttacaagg ttgaagacaa ccctcgtggt gtgaacgtct ttgttagccg tagtcatcca
     6901 gaaatgatca aacgtttaat ggagcaagaa attccagaag tttatgatgg aactgttgaa
     6961 atcatgagcg tggctcgtga agcaggtgac cgtacgaagg ttgctgttcg tagccacaat
     7021 ccaaacgtgg atgctatcgg tacaatcgtt ggacgtggtg gtgctaatat caagaagatt
     7081 actagcaaat tccacccagc tcgttacgat gctaaaaatg accgcatggt accaatcgaa
     7141 gaaaatatcg atgttatcga gtgggtagca gatccagctg aatttatcta caatgccatc
     7201 gctcctgctg aggttgacca agttatcttt gatgaaaacg acagcaaacg tgccttggtg
     7261 gttgttccag ataacaagct ttctcttgcc attggtcgtc gtggacaaaa cgtgcgcttg
     7321 gcggctcact tgactggtta ccgtatcgat atcaagtctg ctagcgaatt tgaagccatg
     7381 gaagacgctg cttcagtaga gttggaagta gaaaacgata ctgtagaaga ataaaagctg
     7441 ctagaggagg gaaagatgaa aacaagaaaa atccctttgc gcaagtctgt tgtgtctaac
     7501 gaagtcattg ataagcgtga tttgctccgc attgtcaaga acaaggaagg acaagtcttt
     7561 attgatccta cgggcaaggc caatggccgc ggcgcttata tcaaactaga caatgcagaa
     7621 gccctagagg cgaaaaagaa gaaggtcttt aaccgcagct ttagcatgga agtggaagaa
     7681 agcttttatg acgagttgat cgcttatgtg gatcacaaag tgaaaagaag agagttggga
     7741 cttgaataag caaaagataa gtaatctctt ggggcttgct cagcgagcag ggcgcatcat
     7801 atcgggtgaa gaattggtgg tcaaggccat tcaagacggc aaggccaagt tggtctttct
     7861 agctcatgat gctggaccca atctgaccaa gaagattcaa gataaaagtc attattatca
     7921 agtagaaatt gtaaccgtgt tttcaacact ggaattaagc atagcagtcg ggaaatcgag
     7981 aaaggttttg gctgtaacag atgctggatt tacaaagaaa atgaggtctc ttatggaata
     8041 gaagaggagg acatgatttg tctaagaaaa gattgtacga aatcgcaaaa gaacttggaa
     8101 aagaaagtaa agaagttgta gcgcgtgcaa aagagttggg cttggatgtg aaaagccact
     8161 catcaagtgt ggaagaagct gtcgctgcaa aaattgctgc cagctttaag cctgcagctg
     8221 ctccgaaagt agaagcaaaa cctgcagcac caaaagtaag tgcagaaaag aaagccgaaa
     8281 aatctgagcc agctaaacca gctgtagcta aggaagaggc aaaaccggct gagccagttg
     8341 ctccgaaaac agaaaaagta gcagtgaaac cgcaaagtcg taatttcaag gctgagcgtg
     8401 aagcccgtgc taaagagcag gcagaacgac gcaagcaaaa taagggcaat aaccgtgacc
     8461 aacaacaaaa cggaaaccgt cagaaaaacg acggccgtaa tggtggaaaa caaggtcaaa
     8521 gcaaccgcga caatcgtcgc tttaatgacc aagctaagaa gcagcaaggt cagcaaaaac
     8581 gtagaaatga gcgccgtcag caagaggata aacgttcaaa tcaagcggct ccacgtattg
     8641 actttaaagc ccgtgcagca gccctaaaag cagagcaaaa tgcagagtac gctcgttcaa
     8701 gtgaggaacg cttcaagcag tatcaggctg ctaaagaagc cttggctcaa gctaacaaac
     8761 gcaaggaacc agaggaaatc tttgaagaag cggctaagtt agctgaacaa gcacagcaag
     8821 ttcaagcagt ggttgaagtc gtccctgaga aaaaagaacc tgcagtggat acacgtcgta
     8881 aaaaacaagc tcgaccagac aaaaatcgtg acgattatga tcatgaagaa gatggtccta
     8941 gaaaacaaca aaagaatcga agtagtcaaa atcaagtgag aaatcaaaag aatagtaact
     9001 ggaataacaa caaaaagaac aaaaaaggca ataacaagaa caaccgtaat cagactccaa
     9061 aacctgttac ggagcgtaaa ttccatgaat tgccaacaga atttgaatat acagatggta
     9121 tgaccgttgc ggaaatcgca aaacgtatca aacgtgaacc agctgaaatc gttaagaaac
     9181 ttttcatgat gggtgtcatg gccacacaaa accaatcctt ggatggggaa acaattgaac
     9241 tcctcatggt ggattacggt atcgaagcca aacaaaaggt tgaagtggat aatgctgaca
     9301 tcgaacgttt ctttgtcgaa gatggttatc tcaatgaaga tgaattggtt gagcgtccac
     9361 cagttgttac tatcatggga cacgttgacc acggtaaaac aacccttttg gatactcttc
     9421 gtaactcacg tgttgcgaca ggtgaagcag gtggtattac tcagcatatc ggtgcctacc
     9481 aaatcgtgga aaatggtaag aagattacct tccttgatac accaggacac gcggccttta
     9541 catcaatgcg tgcgcgtggt gcttctgtta ccgatattac gatcttggtc gtagcggcag
     9601 atgacggggt tatgcctcag actattgaag ccatcaacca ctcaaaagca gctaacgttc
     9661 caatcatcgt agccattaac aagattgata aaccaggtgc taacccagaa cgcgttatcg
     9721 gtgaattggc agagcatggt gtgatgtcaa ccgcttgggg tggagattct gaatttgttg
     9781 aaatctcagc taaattcaac caaaatatcg aagaattgtt ggaaacagtc cttcttgtag
     9841 ctgaaatcca agaactcaag gcagacccaa cagtgcgtgc aatcggtacg gttatcgaag
     9901 cgcgcttgga taaaggaaaa ggtgcggtcg caacccttct tgtacaacaa ggtaccttga
     9961 atgttcaaga cccaatcgtt gtcggaaata ccttcggtcg tgtccgtgct atgaccaacg
    10021 accttggtcg tcgtgttaaa gttgctggac catcaacacc agtctctatc acaggtttga
    10081 acgaagcacc gatggcgggt gaccactttg ccgtttacga ggatgaaaaa tctgcgcgtg
    10141 cagcaggtga agagcgtgcc aaacgtgccc tcatgaaaca acgtcaagct acccaacgtg
    10201 ttagccttga aaacctcttt gataccctta aagctgggga actcaaatct gttaatgtta
    10261 tcatcaaggc tgatgtacaa ggttctgttg aagccctttc tgcctcactt caaaagattg
    10321 acgtggaagg tgtcaaagtg actatcgtcc actcagcggt cggtgctatc aacgaatcag
    10381 acgtgaccct tgccgaagct tcaaatgcct ttatcgttgg tttcaacgta cgccctacac
    10441 cacaagctcg tcaacaagca gaagctgacg atgtggaaat ccgtcttcac agcattatct
    10501 acaaggttat cgaagagatg gaagaagcta tgaaagggat gcttgatcca gaatttgaag
    10561 aaaaagttat tggtgaagcg gttatccgtg aaaccttcaa ggtgtctaaa gtgggaacta
    10621 tcggtggatt tatggttatc aacggtaagg ttgcccgtga ctctaaagtc cgtgttatcc
    10681 gtgatggtgt cgttatctat gatggtgaac tcgcaagctt gaaacactat aaagacgacg
    10741 ttaaggaagt tacaaacggc cgtgaaggtg gattgatgat cgacggctac aatgatatta
    10801 agatggatga tgtgattgag gcgtatgtca tggaagaaat caagagataa gattttttgc
    10861 tcctttctta ggtggtgagg gacgcaagca aaccgatggt ttcattgctt atttttgagc
    10921 ctagggtctc aaaaatcccc tgtgatggga ctgataaatc agttccatca ctttcaccac
    10981 ggcgaaagaa gcagatgact tcaaattgaa cttcgtttca atttaaactg aaaatcaaga
    11041 agtttaaaat agctaggtct gctggcctag cttttggttc aaagtagaga aaggaatatc
    11101 atggcaaatc atttccgtac agatcgtgtg ggcatggaaa tcaagcgtga agtcaatgag
    11161 attttgcaaa agaaagtccg tgatccacgt gtccaaggtg tgaccatcac agatgttcag
    11221 atgctgggtg acttgtctgt tgccaaggtt tattacacca ttttgagtaa ccttgcttcg
    11281 gataaccaaa aagcccaaat cgggcttgaa aaagcaactg gtaccatcaa acgtgaactt
    11341 ggtcgcaatt tgaaattgta caaaatccca gatttgacct tcgtcaaaga cgagtccatc
    11401 gagtatggaa acaagattga cgagatgcta cgcaatctgg ataagaacta aagaagaggg
    11461 gttgcccctc tttttcggct ctttgtcaac tgtagtgggt tgaa
//
LOCUS       DAVBCG010000055        11368 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBCG010000055 DAVBCG010000000
VERSION     DAVBCG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.055"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(39..2036)
                     /gene="metG"
                     /locus_tag="VQ495_001219"
     CDS             complement(39..2036)
                     /gene="metG"
                     /locus_tag="VQ495_001219"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2531180.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2160..3419)
                     /locus_tag="VQ495_001220"
     CDS             complement(2160..3419)
                     /locus_tag="VQ495_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531181.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3421..4122)
                     /locus_tag="VQ495_001221"
     CDS             complement(3421..4122)
                     /locus_tag="VQ495_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531182.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4106..5305)
                     /locus_tag="VQ495_001222"
     CDS             complement(4106..5305)
                     /locus_tag="VQ495_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2531183.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5591..6937
                     /gene="gor"
                     /locus_tag="VQ495_001223"
     CDS             5591..6937
                     /gene="gor"
                     /locus_tag="VQ495_001223"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2531184.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            7134..7670
                     /locus_tag="VQ495_001224"
     CDS             7134..7670
                     /locus_tag="VQ495_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2531185.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7721..7807
                     /locus_tag="VQ495_001225"
                     /pseudo
     CDS             7721..7807
                     /locus_tag="VQ495_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7876..8091)
                     /locus_tag="VQ495_001226"
     CDS             complement(7876..8091)
                     /locus_tag="VQ495_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2531186.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8189..8509
                     /locus_tag="VQ495_001227"
     CDS             8189..8509
                     /locus_tag="VQ495_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531187.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8540..8878)
                     /locus_tag="VQ495_001228"
     CDS             complement(8540..8878)
                     /locus_tag="VQ495_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531188.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(8890..9609)
                     /gene="trmD"
                     /locus_tag="VQ495_001229"
     CDS             complement(8890..9609)
                     /gene="trmD"
                     /locus_tag="VQ495_001229"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2531189.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9599..10117)
                     /gene="rimM"
                     /locus_tag="VQ495_001230"
     CDS             complement(9599..10117)
                     /gene="rimM"
                     /locus_tag="VQ495_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2531190.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10148..10936)
                     /locus_tag="VQ495_001231"
     CDS             complement(10148..10936)
                     /locus_tag="VQ495_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2531191.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
BASE COUNT         3264 a         2502 c         2073 g         3529 t
ORIGIN      
        1 aaaaaaacag aactagtctg aactagtcct gtctactttt acccaatcac acttccattt
       61 ggtacagctg gatcaactgt gagaagggtt aatttgccat catattcagc tgatagaatc
      121 ataccctggc tgacatattt tttcatcatt ttacgtggtt tgaggttagc aacgatttga
      181 actttcttgc cgaccaattc ttgttcattt ggatagtatt ttgcaattcc tgaaagaatc
      241 tgacgatctt ctccatcacc agcatccaag cggaattgaa gcaacttatc tgaaccttct
      301 actttagaca cttctttgac ttctgcgaca cggatttcaa ccttgtcaaa gtcttcaaac
      361 ttgatttcat ccttgtttag tttgagctca acttcgtccg gattccattc tttttcgact
      421 gctggtttat tgccttccat ttgttccttg atataggcga tttcttcttc catatttaga
      481 cgtggaaaga taggtgttcc tttggcaact acagtcacat ctgctgggaa gtcagccaaa
      541 ctcaagtttt caagactaga aacttcttcc aaaccaagtt gagtcaaaac tgcacgacta
      601 gtttccatca taaatggttc aatcaagtga gcaactacac gaatgctggc tgccaagtgg
      661 ctcatgacac ttgccaattg gtcacgaaga gcttcatcct tggccaagac ccatggtgcg
      721 gtctcatcga tgtatttatt ggtacgagag atcagagtcc agactgcttc aagcgcacgt
      781 ggatagtcaa ctgcttccat gtgtgtatgg aagtctgcga ttgattgttc tgcaacctca
      841 gcaagaacat gatcaaattc agtcacacct tctacatagg cagggatttg tccatcaaag
      901 tacttattaa tcatggaaac cgtacggtta aggaggttcc caaggtcatt agccaattca
      961 tagttgatac ggccgacata gtcttcagga gtaaaggttc cgtctgaacc aactggaagg
     1021 ttacgcatga ggtagtaacg aagtggatct agtccataac gctctaccaa catttcaggg
     1081 taaacgacat tcccttttga cttagacatt tttccgtctt tcatgacaaa ccaaccatgg
     1141 gcaatcaaac gatcaggtaa tttaacatcc aacatcataa gaaggattgg ccagtagata
     1201 gagtggaagc gaaggatatc ttttcctacc atatggaaga ctgttccatt ccagaacttg
     1261 tcaaagttac catgttcgtc ttgagcgtag ccaagagctg tcgcatagtt aagaagggca
     1321 tcaatccaaa cgtagacaac gtgttttgga tttgatggga caggcactcc ccatgtaaag
     1381 gttgtacgag ataccgccaa atcttccaag cctggctcga tgaagttgcg tagcatttca
     1441 ttaaggcgac catctggcgt gataaattca ggatgagctt tgaaaaattc gaccaaacgg
     1501 tcttggtatt tgctaaggcg aaggaagtat gattcttcag aaacccattc aacctcatga
     1561 cctgatggag caataccacc agtcacattt ccagcttcat cacggaaaac ttctgccagc
     1621 tggctttctg taaagaattc ttcgtctgat actgaatacc aaccagagta ttcacccaag
     1681 tagatatcat cttgagcaag taagcgttca aagacctgtg cgacaacttt ttcatggtag
     1741 tcatcggttg tacggataaa tttatcgtat gagatatcta gtaattgcca gagttcttta
     1801 actccaaccg ccattccatc aacataggct tgaggtgtaa taccagcttc ttccgctttc
     1861 tgctggattt tctgaccatg ttcatcaaga cctgtcagat aaaatacatc gtagcccatc
     1921 aggcgtttgt aacgtgctag gacatcacat gcgatagttg tgtaggcaga accgatatga
     1981 agtttcccag atggatagta aatcggcgtt gtaatataaa aatttttttc agacataatt
     2041 tttcctttcc aggcaaatga aacctgtttt tctaacactt cattatatca catttttaat
     2101 gaatttcgat agggaaatcc atacaaaaac aagatagacg agtgtccatc ttgttgatct
     2161 cattcataac gaagggcttc aattggatca agtttcgatg ccttgttggc tggcaagact
     2221 ccaaaaatca taccaacact agccgaaact gcaagactaa atagggcaac tgggattgat
     2281 actccaactt ctataccttc tattaaacct tgcagtaaca aacctgctaa ggcagttaaa
     2341 ccacttgcaa ttgtcaagcc aattaagcca cctaacaagg tcaaaatcat ggattcaatc
     2401 aaaaactgaa ttaaaatatt ggcacgtgtt gcacccaaag ctttacgaag accaatctca
     2461 cgagtgcgct ctgtcaccga aaccagcatg atgttcatga caccagttcc tccaacaaag
     2521 agagaaatcc ctgcgatgga actaataatc gtcgtcataa aactaaacga ttgttgaatt
     2581 tctgcaaata caacggactc atctgccacc tggtattctc cctgttgtaa gcctgcaagc
     2641 tctgtcattt ttcgtgccag ttctggaccc agagttgggg ttaaactggt atcattcact
     2701 cgaaagacaa tactagctat ttcatctata ttaaaattcg cagcaaggga gatattggta
     2761 gtaataggca agccaccaaa cccatatatt tttgactttt tagcctccgg actagtataa
     2821 accccaatga cccggtaact aaatccattg acttctacaa ccttgttaat agcctcttga
     2881 ggagattcaa ataaactaat ggacaattcc tcatctagca aaataacact tgcaaactct
     2941 ttgaaatctt gctctctcag actacgacct gcaataattt cattcttaac agcgtccatg
     3001 taagttctgt ttccacctgt caaattagca ttctcaacct ttttatcttg ataggtcaag
     3061 atggcattcg ttgaattggt tacatagtaa ctatccactc ccttcagttt agctgcctct
     3121 tgaacccagg attcttgcgg ttttggcggt tcaacaggaa cttcctcttc ctttccagaa
     3181 accgtaaaag ctgattgttt ctgagtaaaa gacccatctt tacttttttt aggagagaaa
     3241 aagacgctaa tatttttctg agatttagtc atatctttat tgacttgacg agatagggaa
     3301 tcacccaaag ccataatcac aacaactgat gaaacaccga taataatccc aatcatagta
     3361 agcaaagaac gcatcttgtg agccatgata gatgaaaagg caaatttcag attctgcatc
     3421 ttagttttcc tcctttccta actgagcact gtcagacgaa atgaccccat cccgaatgac
     3481 aatctgacgt ttggcatagg cagcaatctc aggctcatgc gttaccatga taatggtttt
     3541 tccttcttta ttcaaatcaa ccaataattg cataatttgg ttacctgttt tggtatccaa
     3601 ggctcctgtc ggttcatccg ctaggataat agaaggattg tttaccaagg cacgcgcaat
     3661 ggctacacgt tgcttttgac caccagataa ttctgaaggt aaatggtgac tacgttctgt
     3721 caattcaacc ttgtctaaat attcctcagc caacttgcga cgttttgaag acgaaactcc
     3781 tgcgtaaatc aagggcaatt ctacattttg cagagcattg agcttcgata gaagaaagaa
     3841 ctgctgaaag acaaaaccga tttgttggtt acggacctta gctagttgtt tttcaccaag
     3901 cccagccact tcttgacctt caagataata ttctccactg gttggtgtat ccaacatgcc
     3961 aatcgtattc atcagagtgg acttaccaga cccagatggt cccatgatgg ctacaaattc
     4021 accctcattc acttctagat tgatattttt gagaacctgc agttcttggt caccattacg
     4081 gtaacttctg aagatatttt ttagactaat tagttgcttc atcagccttc acctcttttc
     4141 cttcttccaa ggaagatgtt ggattactga tgaccttagc accgttcgtt aaaccagaag
     4201 tgatttcttg attttctgcg tcagcatttc ccaatgaaac ctcaactttt ttagcctttt
     4261 gttgttcatc cacaatccag acataatttt tactatcatc cattactaga ctgctaacag
     4321 gaataagaat agccttagtt ttgcttttaa cctcaatgtt gacagaaaaa ccttgtttca
     4381 aatcaccaac ctcgcctgtc acatcaatag tataagggta tttagaacct gtattattcc
     4441 cggctgctgg actagctgct tcaccattgt ttttaggata gtcagaaata tagcttaatt
     4501 tcccagtcca ttttttatca ggatacactt tagaagtaaa gcttacttct tgacctacag
     4561 aaaggttggc tagattgtac tcagacaatt ctcccttgac ttgtaaattt tcattgctga
     4621 caatatgaac cataacttga ctcgcccctg ttggagattt agaaacattg ctattgactt
     4681 cgactacagt tccctctagg gtactgagaa cagttgttgc atccaattga ctttgagcct
     4741 tgcttaattg cgctgcagca tctgcacgcg catcacgggc atcacccaat tgagcatcaa
     4801 tagaagcaac agaatttcca gccactggag ttgggctttg caccgttgca tcttctcctc
     4861 ctactggcgc tggtaactgt ggagcctgag ctgaagcggc ttcatttcgt gcttgattga
     4921 gttcattgat atgacgatct gccttagcta ctgctcgact agctgaatca taggccgcct
     4981 gcgcttctga actactgtac ttgactaaag cctgcccttc gctgacctta tcgcccacag
     5041 aaacaaggat ttcatctaaa tcacccttac tagcatcaaa ataaacatat tgttcatttt
     5101 ttgctgttac tgtccctgac aataaaacag aggatgccac gcttccttcc ttggcaacaa
     5161 caagatgagt aggctcatct tttacagcag tctgagaagg ttgtctaaaa agtaaaatcc
     5221 ccccggcacc caatacaact acactcgcag caccgattgc tgcatacagt tgccactttt
     5281 tagctttacc attctttttc ttcataatga aactcctttt cttttttaca atactttgct
     5341 attataccaa atttccctcc agcaaacaat gcagttcaga tggaaataat ggcagttcag
     5401 gattaaacaa tcgttcggaa ttttgctttt ctattgtact agatatataa tacactatct
     5461 tgacttattt ggcaagcttt tttgcaattt tttgtgcgta gcagattttt gcaatcaaat
     5521 caaaaaaaga ataaaatata actatcaaaa aagacactct actatttaaa agattacaaa
     5581 ggagttttca atgagagaat atgatatcat tgctatcggt ggaggtagcg gaggaatcgc
     5641 taccatgaac cgtgctggtg aacatggagc caaagcagcc gttattgagg aaaagaaatt
     5701 aggtggaacc tgtgtcaacg tcggttgtgt tcctaaaaaa atcatgtggt acggggcgca
     5761 aatcgctgag actttccatc aatttggaga agactacggc tttaagacta ctgatcttaa
     5821 ctttgacttt gcaaccctac gtcgcaatcg tgaagcctac attgatcgcg ctcgttcttc
     5881 ttatgatggt agttttaaac gcaacggtgt agacttgatt gaaggtcatg ctgaatttgt
     5941 agattctcat actgtaagcg taaatggtga actgattcgt gctaaacata tcgtgattgc
     6001 tacaggtgcc catccaagta ttcctaatat tcctggtgct gagctaggtg gctcttctga
     6061 tgatgtattt gcctgggaag aacttccaga gtcaattgcc attctaggcg ctggttatat
     6121 cgccgttgaa ttagctggcg tactccacac ttttggtgtc aagacagatc tctttgttcg
     6181 ccgcgatcgt cctttacgtg gttttgattc ctacatcgtt gaaggtttgg tcaaggaaat
     6241 ggaaagaaca aacttaccac ttcacactca caaagtccct gtcaagttag aaaaaactac
     6301 tgacggcatt accattcatt tcgaagatgg tactagtcac acagctagcc aagttatctg
     6361 ggctacaggt cgccgtccaa acgttaaggg cttgcaactt gaaaaagctg gagtgactct
     6421 gaacgaacgt ggctttatcc aagtggatga ataccaaaat actgttgttg agggaatcta
     6481 tgctctaggt gatgtaacgg gcgagaaaga actgactcca gttgcaatca aggccggacg
     6541 taccctatct gaacgtctct ttaacggaaa aactactgca aaaatggatt actcaactat
     6601 tccaactgtt gtcttttcac accctgctat cggaactgtt ggtttgacag aagagcaagc
     6661 tattaaagaa tacggtcaag accaaatcaa ggtttataaa tcaagctttg catctatgta
     6721 ctctgcttgc actcgcaacc gtcaagaatc ccgtttcaaa ctcataacag ctggttcaga
     6781 agaaaaagtt gtcggacttc atggaattgg ctacggcgtt gatgaaatga ttcagggatt
     6841 tgccgttgct atcaaaatgg gagcaaccaa ggctgacttt gatgcaactg tggcaattca
     6901 cccaactgca tctgaagaat ttgtaaccat gcgttaatcg aaacaaaaga agctgatata
     6961 aatatgtctg cttctttttt aatgattttc gaatcaacaa aaaaattatc caacttgact
     7021 agtcacgaaa atcggctgtt acagtttttg ttaccttgtt tttaaaaaat ggttgacatc
     7081 gactcctata tgagtataat agttatcata cattagaaaa gaggttaact attttgaaaa
     7141 aagctcacgt ttatgctatc cctgctattg gggctgctct cattgctgct ttggcacaaa
     7201 tcagtcttcc aattggacct gttcccttca ctctgcaaaa ctttgcaatc ggcttgattg
     7261 ctactgtctt tagaccgaga gaggctgtac tttctgctgg actctatctt cttctaggtg
     7321 ctatcggtct tcctgtcttt gcaggaggtg gagctggttt tcaggcttta gttggcccta
     7381 ctgcaggcta tctttggttt tatctcgttt actctggact tacttcctct ctaaccaaca
     7441 gcaagagtgg tgttgttaag atttttcttg caaacctctt gggtgatgcc cttgtctttg
     7501 tcggcgggat tctcagcttg catttcctag ctggaatggc atttgaaaaa gctcttgctg
     7561 tgggggttct tccctttatc attccagacc ttggcaaact tctggctatt agttttatta
     7621 gccgtcccct acttcaacgc cttaaaaatc aggcttactt tactaactaa aaaaggatat
     7681 cgagttatca tgactcaata tccttttctt ttattttgaa aacttatact caatgaaaat
     7741 caaagagcaa actagaaagc tagccgcagg ctgctcaaaa cactgttttg aggttgtgga
     7801 tgaaactgac gaagtcagta accatacata cggcaaggca aagctgacgc ggtttgaaga
     7861 gattttcgaa gagtattagt tgcctttaaa ggcatccacc atcgtttgaa attcttcatt
     7921 tgagagagta atccctttgc ccattttagt atggtctgga ctccaagcac gaatatcaaa
     7981 ctttgcaggg gcaccattaa agctcacacg gttaatttcc ttggtccaac ctttttcgtt
     8041 ttcagaaaga gtcaacaagt gctcttcgat ttcaaatgta aattctgcca ttttcttctc
     8101 cttttttagt ttcattagtt tattcgtaaa atcttgtaga ttttaggaaa attttatata
     8161 atattgatat aaaagaaggg aggccaatat gagacataaa ttccagcaag ttctaaataa
     8221 aatacatgat tttttaaatg gatatgacca acctgaccag actgaaacca actcccttac
     8281 agccactatt gaagaggcta tccagaaaca aaccgctgtt caccttatct tgtctgagac
     8341 aagttttaca ggtgatatca tcaaatatga tcagcaaggc cagcaaatta tcgtgaaaaa
     8401 ttttgccaaa aatgtgagcc ggattatccg tataagcgat attcaacgcc tgcgatttgt
     8461 cccctcaact gtccaaacag cccaaaaaaa tagatttaag aaagagtgag atgtagttgc
     8521 ttcatcccac tcttttttct tagcgaattt gttcaaaatg taaatgaact gcaatatgat
     8581 ctccataacc acttctttcc aagtcacgtt gtaaacgata ggaaatgtag tgttctgcaa
     8641 tggtaatgta acctgcgccc aataaacgat gttcaaccat agattgaatc atactgatag
     8701 tcgcacgttc caccttggct tcttgtaaat ccaaaactac cttcttagtg acttgagcaa
     8761 gattttgacg caaatcatct gtcaaaacat aaacagtttg ggctgccttc aagatggctt
     8821 ggtaaatctt atctggatta aattcagcaa tttcgccatt acgtttgatt acttgcatag
     8881 gtttctcctt tattctttgt tttctttgat ttctgccagc attttttctt cttctactgt
     8941 cagttgataa tgttcaagta aatccggtct gcgctcgtag gttttcttta aactctcgta
     9001 caatcgccac tgacgaatct tttcatggtg gccactcatc aatacatctg gcacgaccat
     9061 gcctcgataa tcatagggac gtgtgtactg aggatattct aaaagacctg aagaaaaact
     9121 atcatcttgg tggctagact ccttgccaat cacttctgga atcaggcgaa ctgtagcatc
     9181 aatcatggtc atagctgcca attctccacc agtgaggaca tagtcgccta gggaaatctc
     9241 atctgttacc aaggtcttaa tgcgctcatc ataaccctca tagtgcccac agataaagat
     9301 tagctcttcc tcttgagcca aatcttcagc ataagcctga tcaaactgct ttccagcagg
     9361 atcgaggaga ataacgcgcg gatttttctt ttcaatagca tcaaaggaat cgaaaatagg
     9421 ttgtgctctg agcaacatgc cctgaccgcc tccgtagggc tcatcatcta catgacgggc
     9481 cttttcagca ttttctcgaa aattatgata ctggatatcc aagagccctt tttctcgagc
     9541 ctttccaacg attgagtgct ccagtggaga aaacatctct ggaaagaggg ttaaaatatc
     9601 aatcttcatc gtctaaccct tctaagattt ccacatcgac ccgtttattt ggaatatcaa
     9661 cattgagaac cactggtggg atataaggta aaagcaaatc acgtttgcct tttcgtttga
     9721 ccacccagac atcattagca cctggttgca ggatttcctt gatggttcca accaagctat
     9781 caccctcata gacttccaaa ccgataatct cgtgatagta aaattcacca tcgtctaggt
     9841 cattcaaatc ttcctcagcg accttgagac tatatctctt gtacttttcg atagtattga
     9901 tatggtacat atctttgaat ttaataatgt caaagttctt ctgtttacgg tggctagcga
     9961 tggtcactgt ttggacaaac tgatcttttt catcaaacaa agccagctca gctccttttt
    10021 taaaccgttc ttctgcaaaa tccgtcacag acaagactcg catctcaccc tgtaatccct
    10081 gcgtattaac gattttccca acattaaagt agttcatctt gtctcctgta atctcctttt
    10141 ttccatctta ttctgacaat tctcgaataa tagccgcaat tttttccgat tctgaccatt
    10201 gtaaataatg gtgattccct cctaaaatga gtttagtatt ggaagtccaa tattctgatt
    10261 ctctgtactc tttttctcta taaggctgac aaaaaacaaa tacaggaata tgagcttcta
    10321 tagatacatc ctcaaaatct tcctcagtaa tctctccaga tatctgaaat tctggatctt
    10381 gattttccaa ctctaagcct ttttcttgca ttaattccca gattttttta ttcgtttcag
    10441 gactaaatgt tgcttgagtt aagttcttaa aataaagttc aggaccacac tcgtcaatca
    10501 gcctcatctg ctcttccatt tctggataag gattttctga aaaatcagca aacatgactt
    10561 ttttagttgt cggttcaatt gctactaaag tctgacgctt aattggtttc tcgagtaatt
    10621 tgcaagctaa aattccactc caactatgtg cacaaagtat atattcagaa attcctaatt
    10681 cttcaagtac ttcataaacc gcatctgcaa gattatctag attttttcca gcttggtcat
    10741 gaatcggact cctacctgtg ttcggaaaat caattgtcaa ataaccaatt gtaggaggag
    10801 gtttttcaag tataagtgaa aaattttcat aacttggtag caaacctgct ccgtttagac
    10861 aaactagcac tttcttttgc ttttgataag taacagagag gctaccaatt tctgtagata
    10921 cttcaaacct cttcataaag aaatccactc attctatata atgaattatt aaaaatcctt
    10981 atcttttatt ttatcacgtt ccaaggattt tctcaagttg gaggaagggg acaacatctc
    11041 tactttccct tcaaaaatcc ttccaaatca tgtttatgtt ggtaattaac ggctgcggtt
    11101 ttgtctttct caaagacagt cttggtaagg tcaatatgat taatagctac gattgcgacg
    11161 gtgtagtaaa tgatatcagc cagttctctg gcaagttcct cgttcgaatc ctatcccttc
    11221 ttttcgacca gagcgcctat tcaaaacctc ggctacttct ccgacttcct ccactaactt
    11281 cataaagaga ccttcatcag tccgagactg ctgttaatgt tcgattaagt agtcttggaa
    11341 ttgcctaaac gttcaatctt ttatagta
//
LOCUS       DAVBCG010000056        11285 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBCG010000056 DAVBCG010000000
VERSION     DAVBCG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.056"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            67..715
                     /locus_tag="VQ495_001232"
                     /pseudo
     CDS             67..715
                     /locus_tag="VQ495_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            725..2342
                     /locus_tag="VQ495_001233"
                     /pseudo
     CDS             725..2342
                     /locus_tag="VQ495_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2362..3206
                     /locus_tag="VQ495_001234"
                     /pseudo
     CDS             2362..3206
                     /locus_tag="VQ495_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3274..4775
                     /locus_tag="VQ495_001235"
                     /pseudo
     CDS             3274..4775
                     /locus_tag="VQ495_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            4787..5536
                     /locus_tag="VQ495_001236"
     CDS             4787..5536
                     /locus_tag="VQ495_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2531192.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            5549..6721
                     /locus_tag="VQ495_001237"
     CDS             5549..6721
                     /locus_tag="VQ495_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2531193.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            7116..7979
                     /locus_tag="VQ495_001238"
     CDS             7116..7979
                     /locus_tag="VQ495_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2531194.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            8265..8498
                     /locus_tag="VQ495_001239"
     CDS             8265..8498
                     /locus_tag="VQ495_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531195.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            8520..9197
                     /locus_tag="VQ495_001240"
     CDS             8520..9197
                     /locus_tag="VQ495_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2531196.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            9209..9874
                     /locus_tag="VQ495_001241"
     CDS             9209..9874
                     /locus_tag="VQ495_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2531197.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            9946..11172
                     /locus_tag="VQ495_001242"
     CDS             9946..11172
                     /locus_tag="VQ495_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2531198.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALGTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         3726 a         1520 c         2081 g         3958 t
ORIGIN      
        1 tttctgtgca aaaaatgttt gtagaaacca tgagagtata tgatgaatta ttagagaaga
       61 gtagtcatgg ataggaatat agatcaagaa ttagtaagta ttataatacc aacacataat
      121 aggtatgaat ctctgattag agctgtgaaa agttgtttac atcaaagtta taaaaatatc
      181 gaagttataa ttattgatga taattatagt aatgtaaatt taagaaataa aattatccat
      241 caatttggct ataccagtca tagaattaag ttaattttaa gtaatgaaga tttaggtgca
      301 actaatgcca gaaacatagg tatcaaaaat tctagaggta agtatatatc atttttagac
      361 gatgatgatg aatatatgcc agatcgaatt ttaaagttga tggcttgttt taaaaagagt
      421 agaatgaaga atttagcttt agtttatagt tatggaataa taatttatcc aataggaaat
      481 attttaattt attgagtttc aacgagagaa tattggttac aaagaaatat tattctttaa
      541 acataaagcg gttactatta ataggagaca aatgcaaggc tttaaaagta atcaagaagg
      601 caagagaaga aaaaattttt aacgaatttc tttttcgaaa tatatgttat tatatcattt
      661 tggttttttc tattgtatat atgacaacta tgttcaatta aaatttagaa agtgagaaac
      721 tattgtgtat actattataa aatcaaatat aaaaaaattt agtttattaa cgatatttat
      781 tgttgctggt caattattgc taatttatgc agcaactatt aatgctctgg tgttgaatga
      841 attaattgcg atgaatttag agcggttttt gaaattgtca atctaccaaa tgattgtctg
      901 gtgtgggata atattccttg actgggtagt gaaaaattat caggttgaag tgatccaaga
      961 gtttaatcta gagattcgaa atagagttgc cacagacatc tctaactcta cctatcaaga
     1021 atttcatagt aaatcatcag gaacatatct ttcgtggcta aataatgatg ttcagacttt
     1081 aaatgatcag gcttttaaac aacttttttt agtaataaaa ggaatttctg gtactatatt
     1141 tgcagttgtg actcttaatc actatcattg gtcattgact gtagccacct tgttttcatt
     1201 aatgattatg ctacttgtac caaaaatctt tgcatcgaaa atgcgagaag ttagtctaaa
     1261 tttaactaac caaaatgaag cttttttaaa atctagtgag actatattga atggatttga
     1321 tgtgttagcg tccttgaatc ttttatatgt attgcctaag aaaattaaag aagcaggaat
     1381 tttattaaag atggttatac aaagaaagac aactgtagaa acgttagcag gcgctattag
     1441 cttctttctc aatatttttt ttcagatatc tctcgttttt ttaacaggct atcttgcaat
     1501 aaaaggaata gtgaaaattg gtactattga agcaatagga gcactaacag gtgttatttt
     1561 tacagcgcta ggtgaattag gaggtcaatt atcctctatt attggtacga agcctatttt
     1621 tttaaaattg tattcaatta atccaattga gtcaaataaa atgaatgata tcgaaccaaa
     1681 tgaggtgaat agagattttc cgttatatga agcaaaaaat atttgctata agtatggaga
     1741 taaagaaata ttaaaaaact taaatttttg ttttcaacgt aatgaaaagt atttaatttt
     1801 aggtgaaagt ggaagcggga aatctacatt attaaaatta ttgagtggct ttttgagaga
     1861 ttatagtgga gaattgcgat tctgcgggga tgatataaaa aaaaacctcc tatttaaata
     1921 tggtttcgaa tgttctatat gtagatcaaa aagcttattt gtttgaaggt acgattagag
     1981 ataatatttt attggaagaa aattatactg atgaagaaat actacagtct ttagagcaag
     2041 ttggtttgag tgtaaaagat tttcctaata acattttaga ttattatgtt ggtgatgatg
     2101 ggagattact gtcaggaggg cagaaacaaa aaattacttt agctagaggg ctaattagaa
     2161 ataagaaaat agtattaatt gacgagggaa cttctgctat cgataggaga acttcgttag
     2221 cgattgaacg taagatatta gatagagagg atttgactgt cattattgtt acccatgctc
     2281 cgcatccgga acttaaacaa tattttacta agatatatca atttccaaag gattttattt
     2341 aagaatatag gggagggaaa catgagattc tattgtgaag cttatgaggt aattactgaa
     2401 gagaaaaaag tatactttaa tgatattgag ttagaagtta agtattcctc tgtcgaagaa
     2461 ttgtttataa tatgtgagaa attattagat aagaaaaaag tatcattttt ttatgtatat
     2521 gagaaaccct taaggtatct gttatttgac tatatatttc tactagttct agctaaaaaa
     2581 aatatcccaa tcttagatgg agtttccaat aagcaagttg atccaacgtt gctgcatcac
     2641 ttttctttgg aaattgaaaa gaattttatc gatttttgct ataaaaatat ggatctcatc
     2701 ctaaaaactc aaagtatatc tttgtgtcat agagaagagc ttattattgt agatgtttca
     2761 gatgactcta aatttggtgt tttttacaag cgttttagaa cactggttga taaaaataac
     2821 ggaaaatatg tttgtgttta taatttcagt cgtgtttcag atatactgca aaactggaaa
     2881 aattattgca atcgaaaatt ttcggttacg tttactgaaa gccaatttga attatttaag
     2941 cttttatata atcaaaaaaa ttttaaaaca atttcgttgc tatttggaaa aaaattgttg
     3001 ctggaggaat tatctattat agcgatctta ctaatataga atacttttgt attttttggt
     3061 gggatagtat gtttgggaaa gattctattg gaaaatacgt atatgtagaa gagattagta
     3121 gatgtcattt tttagataga aattattctt tttgttatgg tttacaagat tataaaagta
     3181 aacttattaa atatttttta gaatagttaa tctattcgag agagataaag aaatatggaa
     3241 aatactttat tgaatatcac aggaagcttc gatatggaaa ctaggaatct aattagttat
     3301 tctttaactg atatctttga aacagataaa ataagaattg aacttcttgg agaaatttat
     3361 tataaaaata taaagttgga attgcatgaa ttcgctggat tgtataagat ttatggaatt
     3421 agcttaataa agaatattac cggaatgttt ttaattataa tttttgatac gaagacaaaa
     3481 gaattaaaaa tttttcaaga tataacgact tcatacttta atttatacta tactgtttat
     3541 ggaggtgtgt tctatatata gtacatcttt aaagaaagtg atgaagttat ctcatgttcc
     3601 cgttacctta aataacaaaa aaatacaaga atttatgaga aatggattta ttttagattc
     3661 aaatacttta gtaaccgaaa ttaataaatt agaatatttc tcttacattt ctgtaaataa
     3721 tactcttcgt atatgtggta ttgattataa tgattcaaac aattttacta aagaacaagt
     3781 attaaagaat tgggactcta tgctacgtga atctatatta agagtgtata gtgaagcagg
     3841 agaggctaat attacactat catctggttt tgattcaaat tatatattat atacattagc
     3901 gaattatacg aattcttcaa ttaatgcatt ttgcatagga ggagaaaaag gaattaatga
     3961 gattcctgaa gtaactaaaa ttgcaaaatt ttatggtata aatttattag ttgatactgt
     4021 aatgagccca agatttagaa tattttcccg atatagtatg gagattggaa ggaagtctct
     4081 ttgaatgtgg tgtcatactg cagtactatt taggcaggtt gttgttccaa aaagggaaca
     4141 caagtattct ttgtggcgag tcagcggatg aaataatgac ttttaagtat cattctgtga
     4201 actacaatca attttgtaat gataaacaga agtcagtata ttttagttat tccgactatc
     4261 cattttatgt tacaaattca attgttttaa agaaaaatag cctacttttg catagttttg
     4321 gcattcatcc tagatatcca tataaaatgt ctgagattgt tgaaatgtct aaaaaaatat
     4381 cagatctaaa cgataaaaaa gaatttcaca aaaagaactg tgagttgagg tttcaagata
     4441 gtgttcttga taacataaat agtgtcccgg gtacaactca tttatttagt tgtttaaata
     4501 ttcaaacatt agtaaaaatt attttatata tttttcgata caacagttgt atgaaaatat
     4561 ttaattttag agataaagag cttatctttt ttaataagat tgtaaatggg ctgatacaaa
     4621 atattgagga gaatttagag gatgatattg aaaggatatt gaaatattta tatatatgtt
     4681 tatttaacga aatattcatt attaagaata aggtgaattt ttttgatgat gtggaattta
     4741 atcagacact tagtgagttt ttagataaat tgtagaggga atggttatga gtgacaatac
     4801 taagtatatc tttttatctc cacatttaga tgacgcaatt ttttcctgtg gtgattatat
     4861 ttcaaaatta acttcagaag gagaaattgt acttgttatt actatatttt ctggttaccc
     4921 tttgagccaa cagctacagc cctctgctaa gcaatttcat aagttgtgta accttgggaa
     4981 atatccgatt gaagaaagga agaaagaaga caggttagct tgtgaacgtc tacaatgtga
     5041 ttttagacac ttatcgtact atgaatgtct ctataggaaa gatagaaatg gcaattttct
     5101 ataccgtcat atctatagta aattaaaaaa tgaggacaca ttaaagaatg atatcataaa
     5161 agagctgtta atgcatttag acgacaaatg tgtcgtatat tgtccgcttt cacttggtga
     5221 ccatgttgat catgtttttg tgaatagtat tggaagggcg cttgaattta tgcgttataa
     5281 agtaatatat tatgaggact tcccatatgt aagtgatagt agtatggtta gttatatggg
     5341 aaaaactaaa gaattaaaaa tgtatcaaga ggaacttgat gaaaaacatt atattgatag
     5401 aatatcatca atattgtgct ataaatctca gattttgata atctggaaat cagttgaaaa
     5461 acttttaaac aatattaaag agttgtattt aaggaatggg gcagcttatt ctattagatt
     5521 ttggattaag aaataaggaa gtagttttat gaatatagca gtaattggtt tggggcatgt
     5581 tgggctggcc tacgcgttac tatttgcatc taaatataaa gttgttgcat atgatataga
     5641 ttctgtaaaa ataaataatt taaaaaaggg cactcttcca tctaaaaatg aagagcttat
     5701 gaagtttttt tgcgagaata acttaaatat tacttttttt gatacatttt ctgaaattaa
     5761 aaataatatt gattattata ttattgcgct tccgacagat tatgatgaga aaattggtag
     5821 ttttaataca tatgaaatcg aacaaacggt atcgaagatt ctgagggtaa aacctaatgg
     5881 aaagattatt ttaaagtcaa cagttccgat cggcttttca aacaaattaa aaaggctgtt
     5941 tgatacaaaa aatatcattt ttgtccctga atttttgaga gaaggttgtt ctatatatga
     6001 taatttatat ccaagtcgca tagttgttgg agatgagaca gttgaaggaa gaaaaattgc
     6061 agagttgttc ctttcgatta gtactcatag tactgccaat attaaaaatg ttatgttagt
     6121 ttctcctact gaagcagaag caattaagct tttttctaac acattcttag ctctccgtgt
     6181 tgcttttttt aatgaactag attcttttgc tgagaggaga agtttaaatg ctgaagttgt
     6241 aataaaaggt gtttgtttag atccaagaat tggaaatttt tataataatc cttcttttgg
     6301 atttggagga tattgtcttc ccaaagatac tagacaatta aaaaaagaat ttatagaaat
     6361 aaatgcccca gtgatagaag cgattgatat ttcaaacaca aatagaaaac agttcatagt
     6421 taaacaaata ttggaacgaa agccaaagat agtaggaata tataaattgg ggatgaaata
     6481 taattcagat aattacaaag agtcagctat tttaagtata attaatgaac tattgattgt
     6541 cggtataaaa attttagtat atgaaccaaa tttaaacgtt agtatagata acgtaatttt
     6601 tgaaaagaat tttgaactat ttataaaaca aagtgatcta attgttgcaa atagatggga
     6661 taggggcctt gaagcttata aagataaagt ttacacaagg gggatctgga taagagatta
     6721 actattgata ttaaaaaggc aataatttat aaaatttgta caaaaaaatg ttaaattttc
     6781 acattttcat agtatactgt ttaatagaaa gtaggttctt tgtaaccgct ccattataaa
     6841 atcctttcac tcttccggta tactattagg aaaaacataa ctggaggata ttttgagctc
     6901 acacctcatc gttccagtgg ctgttcccca agctcgtgta ctgcccaaaa aagttagaca
     6961 ttctatttag aacgaggatt gagttctgta ttgtacaagg ctcggtcctt ttagagtcag
     7021 cttaaggctg gctttttcaa tcaccaaagt gtcagaatgt tttgacaaat gaacacaaat
     7081 aatgatataa tatgcaaaag ctaggaggtg gtaggatgat tgaaaaaatg gaactggggg
     7141 aattttacaa ggaattgcgc ttggcgagaa agctcaagca gtcagatgtg gcttgtgctg
     7201 gactaacagc ctctcagttg tccaagtttg aactaggaca gtctatgctg tctgcagaca
     7261 agctgatcct agccatccaa gggatcaata tgaattttga tgagtttggg cacaaactca
     7321 acaactatca agaatctcca catatgcgaa ttggtagaag ggttgtggat tgcttttccc
     7381 atcaggatat agctgcttta gagcagctat tggaggaagt cgatcaagaa cagatggcgc
     7441 agacctatcg tcgtttgaat gccattgtga ttaaaaatgc actccattcc ttagataaaa
     7501 gctacccgct aacagaggag gatagcgagt ttttgactac ctacctctac gctattgagt
     7561 cttggacctg gtttgaactc tatctttttt gcaatactat gcccttcttg agcaatcaag
     7621 atctgatctt tttatcaacc tccttactcg aaaaatccaa ggaatttaaa gagttagtac
     7681 acaatcgatg gtatatgaaa agtggttatc ttaatatcat ctcagaactt atggagcgca
     7741 agctattttc ttatattcca atctttgaag ctgagctgga gcgcatgctc cgtccatacg
     7801 atgtttttga aaaagtattg tggcagtttt taaagaaaat gcagattttt cttcaaacca
     7861 aaggaagcaa tcaaaaagag attgaacact ttatccaatc tctgcaagta ttagaaaatc
     7921 cacaattgac agcccttttt gaattgcgtt tgcagcaata caaagagctt atcgattagt
     7981 tagaaatggg aaaaagataa taatatcagc aacgatcgtc aataatgtta aaacttgttt
     8041 cttcatatta cttccctcct cattagtaat aactttatta taccagggaa ataatcaaat
     8101 ctaccaaaat cgcaaatctg aaatttctat aagaaaaaat atcaaatatg cgattttcta
     8161 aaataagcca atttccgtgt tatactgtcc ttgtaaaaca tttgaagcaa atcctaggtc
     8221 gcagaagtgg tttacaaatg aagataattg aaggagtgta aaagatgtta aatttacaat
     8281 ttgcagaaac aatggaattg acagaagctg agttgcaaga tgttagagga ggcaacctcg
     8341 taaattctat gggaggtggt ggaagaagtg gtatatctgg ttggggcgtc ccaggaattt
     8401 accctggttg gggcaatcaa gggatgagtc ctaatcgtgg agcttttgat tggactatag
     8461 atttagcaga tggattgttt ggccgccgta gaagataggc tcttatagga gataatagga
     8521 tgaagaaaat gaaagaagtg aaatttcacc tagcaactgg cttgttgatt ttgacttatt
     8581 acctcatttt taatgtcaca tcagacttag attttatggt tgctttgtct gataatatgt
     8641 actatgtgtt ccaagtttta ttggtactta ttctgggaac tatagctaca attgcttttg
     8701 tcaaaagtga acattggaaa gagtgtggtc gtttccagtt tcgttggtct tacctgggtg
     8761 ttttccttct ttcttttttc ttattgttcg tgtgggcaaa tctaacaacc tatatctttc
     8821 ctcgtaccca gaatggaagt acagtcgtag aagtagccac cagtttaaca ggaattagct
     8881 attttgttac gcgaatactt tatactagta ttatcgcacc tgtatcagaa gaggttgttt
     8941 gtcgtggtct tttgatgaca agtttatcta aagttaagag atactatctt gatgttcttg
     9001 tttccgctgc catatttggc gctatgcatg ttttgcagta tggttggata acgacagatt
     9061 tcataaagta ttttggaatg ggcttgatat tttgtatgat gtttcgctac acccgatcca
     9121 tctattgggc tatagctctg catgcttcat ggaatagctt cttactcatt gttactttat
     9181 tggtatttgg atattagaaa ggtgatatat gaaaaagata atctcacacc gctactttat
     9241 tatagttttt ctactagtta ttgctgacca gaagttcagt gttttagttt tacgcagcga
     9301 ccttgttgct gggctatctg actttgccta ttatctgtcg gatatgatgt tgaattttct
     9361 tgtggtttta tttgctctta ttgctatgat ttggtcgggg aaatggcaga aaatcaatag
     9421 tagaaagttt aaaggatcct atcttttcta ttccttccta gctctgcttg cttttgttgc
     9481 ttggaatttt gtcacctttt ttcttttccc acctactcga aatgaaattt cttatcaaca
     9541 tgctgctcct acttttacag gagctacggc atttttgatg tatttttttt atcctgtgat
     9601 tgcaggtccc atttttgaag acatgattta tcgtggattg gtgatgacag ctctggaaaa
     9661 aggaaagaaa tgggggctag atgtacttgg ttctgctatt ttatttgggg tctcgcacat
     9721 tagtaatcac ggttgggtct tgacagactt tttcgtctat atgggtggcg gacttatatt
     9781 tgcagtctta tttagaatga caaagtccat ttattggcct attgggctgc atatagtcta
     9841 caacggcatt ggtcagcttt tgatgttact gtaattttgg ttgttagtag tatcgcggtc
     9901 tatttctggg gaaagattcc catttgaact taagaaggag ttgtgatgaa agtatttctt
     9961 caaaatagag attttaggca attaactatc aaccagtgga tttcaacgct tggggatacg
    10021 attttttatc tggccttcct gaattatgtg gcagatgcgt cttttgcccc tttggcgatt
    10081 ttacttatca cgatttcaga aacccttccc caagttctac aaatctttct gggagttttg
    10141 gcggattttc aacatcatcg tgtcttgaaa tacacagtca ttagttttac aaaatttttg
    10201 ctttactcta tagtgtccct atcgctttca gggcagtctt tttccttgtt gctagtaacc
    10261 tttatttgtc tgcttaacct cttgtctgac acattgagtt atttttcagg cgccatgctc
    10321 actccgattt tcattagaat tattgggcaa gaccatctgg cagaagctat tggatttaaa
    10381 cagtcaactg ttagtttagt gaaaacaatc agtaatatcc taggaggagt cttactaggc
    10441 attctatcca tccagtttat ttccttactg aacgctctta cctttttaat cgcattttta
    10501 ggtatcctct tcataaaaac tgacctcttg aaagtagaaa aaacgattag ctatcaagaa
    10561 ggactctctg taaaatcctt ttgccagcat ttgctccaat catcgaaatt gatatggaat
    10621 atgaataagg tgctcttggt tttgtttatt atctctacta gtcaagcagt gataaatgtt
    10681 acggttccta tttccactct atttttaagg aaccagccct ttttgaattt acaaactggt
    10741 caatctcttg ccttgttagc cacactagaa ttatcagccc ttattttggg aagtctgatg
    10801 agtggctatc tgcaacatac catttccata aaaatagccc tgtatgcttc actcctactc
    10861 caattactac tcctagtggg ctttgcgaca gctcattttg gcttgattct gatctttagc
    10921 tttttggatg cctttttcgc gggtattctc tctcctagat tgcaagaact cgtctttaaa
    10981 caaatacctg aggagtcaat gggagcagtt caatcttcta tcggtgccat tacggttgtt
    11041 ttacctagct tatttacaat agctttggga accattgcta ctagctttgg aactctggca
    11101 gttagctttg ttttattact atttcttcta gttgcctttg tcatgcttct gaatattcgt
    11161 gaaagtattt agtggagaat ttctataata tatgtttgag actggattgc cagtcttttt
    11221 atgatggtca aaatcaacag actttttctc ttttctgaat gtgttatagt agactgaatc
    11281 taaaa
//
LOCUS       DAVBCG010000057        11120 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBCG010000057 DAVBCG010000000
VERSION     DAVBCG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.057"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VQ495_001243"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VQ495_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2531199.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VQ495_001244"
     CDS             2483..4144
                     /locus_tag="VQ495_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2531200.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPIIGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VQ495_001245"
     CDS             4213..4992
                     /locus_tag="VQ495_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2531201.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VQ495_001246"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VQ495_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2531202.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..7561
                     /gene="murN"
                     /locus_tag="VQ495_001247"
     CDS             6329..7561
                     /gene="murN"
                     /locus_tag="VQ495_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2531203.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7640..8341
                     /locus_tag="VQ495_001248"
     CDS             7640..8341
                     /locus_tag="VQ495_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2531204.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8381..10228
                     /gene="uvrC"
                     /locus_tag="VQ495_001249"
     CDS             8381..10228
                     /gene="uvrC"
                     /locus_tag="VQ495_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2531205.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10218..11051
                     /locus_tag="VQ495_001250"
     CDS             10218..11051
                     /locus_tag="VQ495_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2531206.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
BASE COUNT         3347 a         1948 c         2656 g         3169 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa gatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt
      181 ggctcggtta ttgtttgaga gagggattca ggaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctc gtttatggag actatgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgctgagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag gaatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatgcct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ccaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gccgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccccatcg agacctcttc atcgcctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt
     1741 gatgatggtg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaaga cctattatct
     1981 gacaggttat gggactagag atcagtttgc taaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtatcagaa cttttagaat aagagggtag aattgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgccattggt gctctacctt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttgatatt gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa
     3301 cgaaattctc ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagaca ggtcgtatgg gtgaacctat cattggactt cgtaagatgt cgattggtcg
     3421 ccatcgttat gtagaaatca aggatgggga cctggtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtgga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacaca tcttcattcc
     3721 taaaaagggg acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag atcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcatacgcgc ggatttgttt atctcaagaa
     3961 gagtcgcgat attctccgtg aaagttcaga attgattaac caaacggtag aagattatct
     4021 tcaaggagat gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa
     4081 gtatctcttt gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt acaccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccacagg ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggttacggc tgcttcaaac tggctcttgc gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattag agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagcgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga gttagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acctttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat
     6121 gcctttgagc gaggtatggt ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgccca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcttgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccttatgaaa cttatcaaac
     6661 ttttgatagc caaggtaatc caatagatgc tgagaaaaaa agtattattc aaggtttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata
     6961 tagtgataaa agtttagaat attatgagca tttttatgat acttttggag aacaagcgga
     7021 gtttctcata gcaagcttga atttttcgga gtatatgagc aaattgcaag gtgaacaaag
     7081 taaactagaa gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga
     7141 gaaaaaacaa aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa
     7201 agcagaagcg cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctgggag
     7261 tttatttgtt tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt
     7321 taataagttc tatgcccctg cactgcttca aaaatatgtt atgttggaaa gcataaaacg
     7381 tggaatacct aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt
     7441 tttgcgtttt aaacagaatt ttaatggcta tattgtacgc aaagcaggta ctttccgtta
     7501 ccatccatcg cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta
     7561 agatgaaaaa gtcagtattt agatttcttt tagcttcttt tagtaaaata attcttattt
     7621 gctagaaagg tggagagaca tgcgctggct ttttcgtttg ataggggctt tcttttcttt
     7681 tgtgtggcgt ttgttttggc gtctggtttg gatagttgtg ctcttatgtg tgcttgcttt
     7741 cggacttctc tggtatctga acggggattt tcaaggagcg ctaaagcaag cagaacggtc
     7801 agtaaaaatg ggtcaacaaa gtattgacca atgggagaaa acagggcaac tgcctaagtt
     7861 aagccagaca gatagtcacc agcattctga aggaaggtgg gcacaggcct ctgctcgtat
     7921 ttacctggat ccgcagatgg attcacgctt tcaagaggct tatttagaag caatccagaa
     7981 ctggaatcaa actggtgctt ttaactttga actcgtgact gagtctagta aggcggatat
     8041 tacggctacg gagatgaacg acggaggcac tcctgtggca ggagaggcgg aaagtcaaac
     8101 taatctctta acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt attatttgtc
     8161 caatccatac tatggctact cctatgaacg ccttgtccat acggcagaac atgagttagg
     8221 tcatgcgatt ggcttggacc atacagatga gaagtctgtc atgcaaccag caggttcctt
     8281 ttatggtatc caggaagagg atgttgcaaa cctccgaaaa atatatgaga ctagtgagta
     8341 gggtactatc tttccctact ttttttgcta taatggaact atgaacaact tgattaaatc
     8401 aaaactagag ctcttgccga ccagccctgg ttgctacatt cataaggata aaaatggcac
     8461 cattatctat gtaggaaagg ctaaaaatct gcgtaatcga gtacggtcct attttcgtgg
     8521 aattcatgat accaagacag aggctctggt gtctgaaatt gtggattttg aatttatcgt
     8581 cactgagtcc aatattgagg cgcttctgct ggaaatcaac ttgatcaagg aaaataagcc
     8641 caagtacaat atcatgctca aggatgacaa gtcctatcct ttcatcaaaa tcaccaatga
     8701 gcgctatcca cggcttatca ttactcgtca ggtcaaaaag gacggtggtc tttattttgg
     8761 cccttatcca gatgtggggg cagccaatga aatcaagcga cttctagata ggattttccc
     8821 ttttcgaaaa tgtaccaacc cgccctctaa ggtctgtttt tattaccata tcggccagtg
     8881 tatggcccac accatctgta agaaggatga ggcttatttc aagtctatgg ctcaggaggt
     8941 gtctgatttt ctgaaaggtc aggatgacaa aatcatcgat gatctcaaga gtaaaatggc
     9001 agtagcagca caaagtatgg agtttgaacg tgcggcggaa taccgtgacc tgattcaggc
     9061 tattggaacg cttcgaacca agcaacgggt catggctaag gatctccaaa atcgcgatgt
     9121 ctttggctac tatgtggata agggctggat gtgtgtgcag gttttctttg ttcgtcaggg
     9181 caagctcatc gagcgcgatg tcaatctctt cccctacttc aatgatccag atgaggattt
     9241 cttgacctat gtgggacaat tctatcaaga aaaatctcac ctagttccca atgaggtact
     9301 gattccgcag gatattgacg aagaagctgt caaggctttg gtggatacca agattctcaa
     9361 accccaacgt ggagagaaaa aacagctggt caatctagcc ataaaaaatg cacgtgttag
     9421 tctggagcag aaatttaacc tgctagaaaa atcagtcgaa aagacccaag gagctattga
     9481 aaatctagga cgtttgctcc aaattccgac tccagttcgc atcgagtcct tcgataactc
     9541 caacatcatg gggaccagtc ctgtttcagc catggtggtc tttgttaacg gcaggccaag
     9601 taaaaaagat taccgtaagt acaagataaa aaccgttgtt gggccggacg actatgctag
     9661 tatgagagaa gtcattcgaa gacgctatgg tcgagtacag cgtgaggctt tgactcctcc
     9721 agatttgatt gtgattgatg gggggcaagg tcaagtcaat attgctaagc aagtcatcca
     9781 agaagaactg ggcttggata ttccaattgc aggtctgcaa aagaatgata agcaccaaac
     9841 ccatgaattg ctctttggag atccgctgga agtggtggac ttgtctcgca attctcagga
     9901 atttttcctc ctccaacgca ttcaagatga ggtccaccgc tttgccatta ccttccaccg
     9961 ccaactgcgc tccaaaaatt ctttctcatc tcaattggat gggattgatg gtctgggacc
    10021 taaacgcaag caaaatctca tgaggcactt caagtctctg accaaaatca aggaagccag
    10081 tgtggatgag attgtcgaag ttggagtacc aagagcagta gcagaggctg tgcaaagaaa
    10141 gttgaacccg caggaaacag aaatcttgct tcaagtggcg gaagaaagag tagattacca
    10201 aacggaagga aaccacaata aaccataaaa tcgcaatttt atcagatatt catggcaatg
    10261 cgacggcgct agaagcagtg attgcagatg ctaaaactca aggagccagt gaatattggc
    10321 tcatgggaga cattttcctc cctggttcag gtgcaaatga cttggtcgct ctgttaaagg
    10381 aacttcctat cacggcaagt gttcgaggca attggaatga ttgtgtcctt gaggctttag
    10441 atgggcaata tggcttagaa gacccacagg aaatccagct catgcgaatg actcagtttt
    10501 tgatggagcg aatggatcct gcaacgactg tctggctacg aagcttgcct ttgctggaaa
    10561 agaaagaaat tgctggactg cgcttttcta tctctcataa tttgccaaat aagaactatg
    10621 gtggtgactt gctagttgag aatgatacag agaaatttga ccaactgcta gatgcggaaa
    10681 cggacgtggc agtttatggt catgttcaca agcagttgct tcgttatgga agtcaagggc
    10741 aacaaatcat caatccaggg tcgattggca tgccctattt taattgggag gcgttaaaaa
    10801 atcaccgtgc ccagtatgcc gtgatagaag ttgaagatgg ggaattggtc aatatccaat
    10861 ttcgtaaagt tgcttatgat tacgaggctg agttagaatt ggccaagtcc aaggggcttc
    10921 cctttattga aatgtatgaa gaactgcgtc gtgacgataa ctatcagggg cacaatctgg
    10981 aacttttagc cagcttaata gaaaagcatg ggtatgtaga ggatgtgaag aatttttttg
    11041 attttttgta agagtttcct aaaatagcca atgcaaacta aaaaagcgat tggctggtcc
    11101 aatcgctttt agtatatctt
//
LOCUS       DAVBCG010000058        11081 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBCG010000058 DAVBCG010000000
VERSION     DAVBCG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.058"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(6..890)
                     /locus_tag="VQ495_001251"
     CDS             complement(6..890)
                     /locus_tag="VQ495_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2531207.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(894..1280)
                     /locus_tag="VQ495_001252"
     CDS             complement(894..1280)
                     /locus_tag="VQ495_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2531208.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1273..2616)
                     /locus_tag="VQ495_001253"
     CDS             complement(1273..2616)
                     /locus_tag="VQ495_001253"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2531209.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2698..3582)
                     /locus_tag="VQ495_001254"
     CDS             complement(2698..3582)
                     /locus_tag="VQ495_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531210.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ495_001255"
     CDS             complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQ495_001255"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2531211.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ495_001256"
     CDS             complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQ495_001256"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2531212.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ495_001257"
     CDS             complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQ495_001257"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2531213.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ495_001258"
     CDS             complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQ495_001258"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2531214.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10565..10831)
                     /locus_tag="VQ495_001259"
     CDS             complement(10565..10831)
                     /locus_tag="VQ495_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531215.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(10915..11081)
                     /locus_tag="VQ495_001260"
                     /pseudo
     CDS             complement(10915..11081)
                     /locus_tag="VQ495_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3095 a         2539 c         2193 g         3254 t
ORIGIN      
        1 cttctttaca aaccaactat agttgacaaa gaacctaaaa tcaattgata acacaaggtc
       61 aggtcggtca actctttcaa ctgaagccct gtcaactctt cccatttatc aatcttgtat
      121 tggagagaat tgcggtgcag atagagttgc tgggctgttt tagtgagaac agcactattt
      181 tcccaaagag agagaatgat ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga
      241 cattctttaa ttggcttcaa gtccacgagt ctttctccca tactccaaag atagagctga
      301 gaaaaagtat gaacaccttg gtgaccctga cgccaccatg tcttgaacaa atcccgctca
      361 gctttgatta agtctgatag ggcttgatgt cctgtctgag accaaacctg acccaacatg
      421 atagaaagac gaagtccaaa gtcatactca actgcttcaa tcatatcact taaaatatct
      481 cttacagaag tgtatttgtc ttgttgaagc acgaaaacat aatcctgagc tccgacctgt
      541 aacactgtct gacaattcgg aaaaagagtc cgcatcatat ctagccaaga agccagattt
      601 tcctgctgaa aataagaaag atggcaataa accaactgaa tctttttaaa aacttgcggt
      661 gcctgtccct tgccttcaac cagataggaa taccaagggt ttagcgaacg agcctgctcc
      721 tgctgggtca aaagggcaac caactgcttt tcacgctcgc tgagcccagc ttcctccagc
      781 aaaatccact gctgagaagc taaaggaagc gtgagatagc cctctttctc tactggttgg
      841 tctgaaatcc gagcctcagg aaaccagtct tgtagttctt ttgccctcat gttctagccc
      901 tccacttttt ggatgcacca tgaaaccaaa ctctcaagac gttccagatt ctcagtcata
      961 tggagatagc ccataactgc ttcaaatccc gtggacatac gataagtcac gacatctgca
     1021 tttttagcct ttgtgtgact attggtattg cggccacgtt tgtagatttc ttcttctttt
     1081 tccgttagga tctgctcctc caacatgaga gaaatcaggc gagcttgagc cttggctgac
     1141 acgtacttgg tcgcctcttg gtggagttta ttgggcttgg tcataccttt gaggatgagg
     1201 tggcggcgaa tatacataga atacaccgca tctccctcaa aggctagcgc aatcccgtta
     1261 atgagattga catcaatcac gtgtccacct cactccatcc ttggtatcaa ggagcttaat
     1321 tccttgagta accaattggt cacggatttg gtctgctgtc gcaaagtcac gattggcacg
     1381 cgcctcttgg cgtttttgaa tcaagtcttc aatctctgca tccaaaactt cctcaacaaa
     1441 gacaattcca aaaatttcta acatatctgc aagagcttgc ttgacacttg catcatagtt
     1501 ccctgagttg atccatttgg ccatttcaaa gacaactgtg ataccgttgg cagcattaaa
     1561 atcttcatcc atagctgcta caaacttatc tttaaagttt tgtaactctt gggcatccac
     1621 gtttcctgta aatggttgtt cgtaagtatt cttcagatac ttgagattgg tctcggcatc
     1681 gcgaactgcc ttttccgtga agttgatagg cttacggtag tgctgggtcg caaagaagaa
     1741 acgaagtact tgcccatcaa gagttttaag ggcatcgtgt accgtaatga agttacccaa
     1801 ggacttagac attttgacat tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc
     1861 aaaagccttg cctgttttag cttcagattg ggcaatttca ttggtgtggt gtggaaactc
     1921 taggtcagct ccaccaccgt gaatatcaat ggtatcacct aaaatctctg tcgacatgac
     1981 tgaacactca atatgccaac ccggacgtcc aggtccccaa ggactatccc aagaaatctc
     2041 acctggtttg gaagatttcc atagagcaaa gtctacagga ttttccttac gagccgtttc
     2101 ttcatcggta cgacctgaag cacctagctc caaatcttcc aaggttttat tagccaattt
     2161 agcatagttg tgggattttt ctacacggaa atagacatcc ccttgactct cataggcaaa
     2221 gcctttctcg atcaagcctt ccacaaaacg gatgatgtct gccataaact ccactacacg
     2281 cggatggcga gtcgcaggtt tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat
     2341 gtacttatcc gcaacctcct gaggcgtgat gccttcttcc ctggcacggt tgataatctt
     2401 atcatccaca tccgtaaaat tggaaatata ggcaacctcg tacccacgat actcaaaata
     2461 gcgacgaatg gtatcaaaag ctaccgttga acgagcattc cctacatgaa tatagttgta
     2521 caccgttggc ccacaaacat acatcttgat cttgccgtcc tcaatcggga caaattctcg
     2581 caaatcacga gacatggtgt catagatttt aatcataaat cataatcaag aaagctgaaa
     2641 tccaagaaca attagtttca tcactaaaag ttcaagtaaa tttcagtccg aatatctcta
     2701 cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgagtct gtttgacaaa
     2761 gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa
     2821 ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt agcttccata
     2881 gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg tattagtcga
     2941 taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat atagtcggct
     3001 ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc
     3061 ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac tatctgctaa
     3121 caaaacttca atatgggaaa catttgctaa cggataaggt cttctatcct tacctaacca
     3181 agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc
     3241 taaaaaaata cgttctgtct gaaaagtgac tgatcgaatg gggaaagaag ctgtttctct
     3301 ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag
     3361 tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa caagttcgcc
     3421 tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag
     3481 atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc
     3541 agatagctcc tcttggctta acttgtttct tacttgaatc atataggtat cctctacaaa
     3601 ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata atactctcgt
     3661 ttttcttcga cttcgtgaat gacaggctca tctttcttac catgaagacg gacaatcttg
     3721 gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca
     3781 ccgaccttgg cattttcacc aatttccaca ggtccgataa cttgggcatg ggctgatatg
     3841 agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccaaga
     3901 gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga
     3961 ccagaaccat ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc
     4021 tgagtccaaa agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gccgtgcttc
     4081 cagataaaat gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa
     4141 acctccaaag tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgcgc
     4201 caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc tgtaaattct
     4261 ttcttaggtt tgtgatcctt gtgatgacgt gggcggtgag gacgctcaga cttttcacct
     4321 ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata
     4381 cggccttttt catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa
     4441 tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc
     4501 ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa
     4561 acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata
     4621 gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca
     4681 cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc
     4741 ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctagacga
     4801 acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga
     4861 gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg
     4921 gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc
     4981 aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca
     5041 ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca
     5101 cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata
     5161 gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca
     5221 ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat
     5281 tgagggaagt tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg
     5341 atttgagttt ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga
     5401 gtaaagagac ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg
     5461 atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact
     5521 tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct
     5581 tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt
     5641 gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag
     5701 gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt
     5761 tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg
     5821 tgccctttaa gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct
     5881 accatgttga tagcgtgctt agttccagct actgtcaatt caagaagaga ttgctctgct
     5941 tgttcttgac ttgggttgat gatgatttgg tcatctacat atcccacttg taccccagca
     6001 attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca
     6061 gccattggtg cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca
     6121 ttacggaaac cttccgcaaa cataggacga atcggacggt caatcaaacg cgctgtcaag
     6181 gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc
     6241 gcatacattt tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc
     6301 ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca
     6361 tttgcttgct tagcaacctg accagtctct acaattaact cacgacccgc aaaagtcgtt
     6421 tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct
     6481 acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc
     6541 ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca
     6601 caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg
     6661 tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca
     6721 agtcatcaag ataccaagcc gtcaagcaac tcaaaggaaa ataggaaatc gaacgacgga
     6781 gcgactgctc ctaggcagat ttatcttttt ccacagagtt gtaggcgtgt tcagttttca
     6841 agatacatca ttagaaaggt ttaatattaa agtatctaaa cctttcacgc taatcgctat
     6901 cgggcgatta gctaaatgct ttactaactc tttcgtcaaa taacatcgat ttgactcact
     6961 cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat
     7021 caagtacgta cagaatctat tttatcatat ttttcttaaa aagtgaggtc tttaccatta
     7081 aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg
     7141 tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc
     7201 gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca
     7261 taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct
     7321 ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag agcctgattt
     7381 cgattcagaa ttggcataat tcctgcatga ataggaacat caatcccagc caagatacac
     7441 ttgtcctgaa aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa
     7501 cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct
     7561 ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg ttccttgata
     7621 aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc tggaataata
     7681 tccccacgaa gagccaagat tttctgaacc ccaactttgt ccaagtcagc aatagtttca
     7741 gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaag
     7801 tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg
     7861 ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata
     7921 atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt
     7981 tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg
     8041 agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc
     8101 aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa
     8161 actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca
     8221 tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt
     8281 ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg
     8341 tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga
     8401 gtgtggattt gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc
     8461 cagtcaaggt aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca
     8521 gcctcatcaa tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg
     8581 atagcaaggg cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac
     8641 cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacgactt
     8701 tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg
     8761 attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt agagaagagg
     8821 tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga
     8881 acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca
     8941 aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggca
     9001 cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg
     9061 aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg
     9121 tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca
     9181 aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag
     9241 tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag tgctggttca
     9301 aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg
     9361 atgttttcag ctggaatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag
     9421 atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa gccaccttta
     9481 acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc tactggtaag
     9541 tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag caagacttca
     9601 agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca
     9661 gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc accaagttca
     9721 gccaatttag caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct
     9781 ttcacgcctt cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga
     9841 cgagtgttca atccaagttc tttggcttct tggaactcat cgaaaatctt gtgaccagcc
     9901 aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac
     9961 catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa
    10021 gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg caccacgttg
    10081 aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga tggaatttca
    10141 gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt tgctgctgct
    10201 aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattca
    10261 cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa
    10321 tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg
    10381 gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa
    10441 cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa tactaaaaca
    10501 tgttattagt aattcttata agtgactatg acctgttatt agaaaagact ataactgatt
    10561 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactggtta
    10621 gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtttccatc ctagctatga
    10681 caggttgact cactccactg acttcttcca gctttttctg actgattcct tgttcatacc
    10741 tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg atttcctcct
    10801 tgctaaagag ctcagatgga catccttcca attactccca atagcactat tcttcatcac
    10861 ttaaccctct ttcttttacg tctatgtatt tttaaaagaa tgagcgaatt atgattcgat
    10921 agattgacca gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct
    10981 agggtaaagc agatcctgct tgcttaaccc caattttcca cgatgaatcc aatagtaaat
    11041 ggttgaaatt cccacgttaa cccctttagc catcaccatc a
//
LOCUS       DAVBCG010000059        11052 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBCG010000059 DAVBCG010000000
VERSION     DAVBCG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.059"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            28..492
                     /locus_tag="VQ495_001261"
     CDS             28..492
                     /locus_tag="VQ495_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2531216.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            650..1075
                     /gene="lacA"
                     /locus_tag="VQ495_001262"
     CDS             650..1075
                     /gene="lacA"
                     /locus_tag="VQ495_001262"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2531217.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1106..1621
                     /gene="lacB"
                     /locus_tag="VQ495_001263"
     CDS             1106..1621
                     /gene="lacB"
                     /locus_tag="VQ495_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2531218.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1632..2561
                     /locus_tag="VQ495_001264"
     CDS             1632..2561
                     /locus_tag="VQ495_001264"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2531219.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2563..3543
                     /gene="lacD"
                     /locus_tag="VQ495_001265"
     CDS             2563..3543
                     /gene="lacD"
                     /locus_tag="VQ495_001265"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2531220.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3777..4613
                     /locus_tag="VQ495_001266"
     CDS             3777..4613
                     /locus_tag="VQ495_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2531221.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALIQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4649..4966
                     /locus_tag="VQ495_001267"
     CDS             4649..4966
                     /locus_tag="VQ495_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2531222.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            4968..6662
                     /locus_tag="VQ495_001268"
     CDS             4968..6662
                     /locus_tag="VQ495_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2531223.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6871..8277
                     /gene="lacG"
                     /locus_tag="VQ495_001269"
     CDS             6871..8277
                     /gene="lacG"
                     /locus_tag="VQ495_001269"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2531224.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8320..8502
                     /locus_tag="VQ495_001270"
     CDS             8320..8502
                     /locus_tag="VQ495_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2531225.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8780..9541)
                     /locus_tag="VQ495_001271"
     CDS             complement(8780..9541)
                     /locus_tag="VQ495_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2531226.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9723..10685)
                     /gene="nrdF"
                     /locus_tag="VQ495_001272"
     CDS             complement(9723..10685)
                     /gene="nrdF"
                     /locus_tag="VQ495_001272"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2531227.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10873..>11052)
                     /locus_tag="VQ495_001273"
     CDS             complement(10873..>11052)
                     /locus_tag="VQ495_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531228.1"
                     /translation="SDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTD
                     DGGEVGANQCESCVI"
BASE COUNT         3455 a         1891 c         2253 g         3453 t
ORIGIN      
        1 gaaactagca aggtcaggcc ttactttatt ggtggacatc caggatttaa ttgtcttcta
       61 cttgatgatg aagtctatga agattattat ctagagtttg agaaagaaga gatttgctct
      121 gttccacgtc ctttcccaga aacaggtatg ttggatttcc aagatagaag tccatggcta
      181 gagggacaaa aagaaataga tcttagttat gatcttttca gcacagatgc agtgactttg
      241 gatgaattgc aatctcgaac aattgccctt cgttctctta aacatgataa gggattgaaa
      301 gtgcattttg cagaatttcc aaacctcatc atctggtcaa ctttgaacaa gggacctttc
      361 attacctttg aaccatggtc tggcttgtca acattccttg aagaaggaga tcatttagaa
      421 gataagaaga atgtttgtct cttagaagcc aatcaggttg aagaattagg gtttgagata
      481 gaagttttat aaaaaaagta aaaaataaat aacaaaaaca aacacaaact gttgacttct
      541 tcaaacaata gtagtatatt atgtttgtga ggactaattg actgtttttt aaagacagcc
      601 aatacactgc tggaatccag catagaaaaa ataaaaagga gtatacaata tgtctattgt
      661 tatcggtgca gatgctgcag gtttgagatt gaaagaagtt gtgaaagact tcttagaaaa
      721 agaaaacttc caccttgtgg atgttacagc tgaaggtcaa gactttgttg atgtgactct
      781 cgctgttgct gcagaagtaa acaaagaaga acaaaacctt ggtatcgtga ttgatgctta
      841 tggagctggt ccatttatgg ttgcaactaa gatcaaagga atggttgctg cagaagtatc
      901 tgacgaacgt tcagcttata tgactcgtgg ccacaacaac tcacgtatga tcactatggg
      961 ggcacaactt gttggtgatg aattggctaa aaacatcgct aaaggatttg ttaatggtaa
     1021 atacgacggt ggacgtcatc aaatccgggt tgacatgttg aacaaaatgg gctaattaga
     1081 ttacagtaag aaaggtaagt taaaaatgag aattgcaatt ggatgcgacc acatcgtaac
     1141 agatgaaaaa atggcggttt cagaattttt gaaatcaaaa ggatatgaag tcattgactt
     1201 tggtacatat gaccatacac ggactcacta cccaatcttt ggtaaaaaag taggggaagc
     1261 tgtaactagc ggccaagctg atcttggggt atgtatctgt ggtactggtg ttggtatcaa
     1321 caacgctgta aataaagttc caggtgttcg ttctgccttg gttcgtgata tgacaacagc
     1381 cctttatgct aaagaacaat tgaacgctaa cgttatcggc tttggtggta agattactgg
     1441 tgaattgctt atgtgtgata tcatcgaagc tttcattcat gctgaataca aaccatctga
     1501 agaaaacaag aaattgattg cgaaaattga acaccttgaa agtcacaatg ctcaacaaac
     1561 agacgcaaac ttctttacag aattccttga aaaatgggat cgcggagaat accacgacta
     1621 agaggtgacc tatgatttta acagtcacaa tgaacccatc cattgatatt tcctatccct
     1681 tggatgagtt gaagattgat actgtcaatc gtgtggtgga tgtaaccaaa acggctggtg
     1741 gtaagggact taatgttacc cgagtacttt cagaatttgg tgattctgtt cttgctactg
     1801 gtttagtggg tggcaaactt ggtgagtttt tggttgaaca tatcgatgat caagtaaaga
     1861 aagatttctt ttctattcag ggagaaactc gtaactgtat cgctattctc cacggtgaca
     1921 accaaacaga agttcttgaa aaaggtcctg aagtattgga acaggaaggt caagactttt
     1981 tggaacattt caaaaaactc ttggagtcag ttgaagtagt agccatctca ggtagtctgc
     2041 cagctggcct tccagttgat tactatgcga gcttggtaga gcttgctaat caagctggca
     2101 agctagtagt cttggactgc tctggtgcag cacttcaggc ggttcttgaa tcaccccata
     2161 aaccgacagt aatcaaaccc aataatgagg aattgtctca gcttctagga agagaagttt
     2221 ctgaggattt ggaggaatta aaagaagtcc ttcaagaatc tttgtttaca gggattgaat
     2281 ggattatcgt ttcacttggt gccaacggta cttttgccaa acatggtgac actttctaca
     2341 aggtagatat tcctagaatt caggtggtaa atcctgttgg atctggagac tctactgtgg
     2401 caggaatttc ttcaggactt cttcacaaag aatcggatgc aggattactc atcaaggcaa
     2461 atgtccttgg tatgctcaat gctcaagaaa aaatgactgg tcatgtcaac atggccaact
     2521 atcaagctct atatgatcaa ttaatagtaa aagaggtata aaatggcttt aacagaacaa
     2581 aaacgtgtac gcttagaaaa actttctgat gaaaatggta tcatctcagc tcttgcattt
     2641 gaccaacgtg gtgctttgaa acgcctcatg gctcaacacc aaacagaaga accaactgtg
     2701 gcccaaatgg aagaacttaa aatcttggta gcagatgaat tgactaaata cgcttcatct
     2761 atgcttcttg accctgagta tggacttcca gcaactaagg ctcttgatga aaaagctggt
     2821 cttctccttg cttatgaaaa aacaggttat gacacaacaa gcacaaaacg cttgccagac
     2881 tgcttggatg tttggtctgc aaaacgtatt aaagaagaag gtgcagatgc agttaaattc
     2941 ttgctttact atgatgtaga tagctcagac gaactcaatc aagaaaaaca agcctacatc
     3001 gaacgcatcg gttctgagtg tgtggctgaa gatatcccat tcttccttga aatccttgct
     3061 tacgatgaaa aaattgcgga cgcaggttct gtagaatacg ctaaagtaaa accacacaaa
     3121 gttatcggtg ctatgaaagt cttttcagac ccacgtttta acattgatgt cttgaaagtt
     3181 gaagttcctg ttaacattaa atatgttgaa ggcttcgctg agggtgaagt ggtttacaca
     3241 cgtgaagaag cagcagcctt cttcaaagcg caagatgaag caacgaactt gccatacatc
     3301 tacttgagtg ctggtgtatc agctaaactc ttccaagata ctcttgtatt tgctcatgaa
     3361 tcaggtgcaa actttaacgg agttctttgt ggtcgtgcta catgggcagg gtcagttgaa
     3421 gcttacatca aagatggtga agcagcagct cgcgaatggc ttcgcacaac tggatttgaa
     3481 aacattgatg agctcaataa agttcttcaa acaacagcga cttcatggaa agaacgtgtg
     3541 taagaaagtc ctcctagttt aggaacatga atctaaaaaa atttaaaaaa agttgtatgt
     3601 aaaggtttac aaaataactt acttgtgcta tacttaaatc acaagttaat acaaggtgag
     3661 tgttactaag taatattagg catgatcaca ggtgaattag aaatcagctg attttctagt
     3721 tcatttgtgg tcattttttg tactcatata cctttaagat ataaaaggag gttgacatgt
     3781 atcgaattct aaatccaatg aatcacaatg tctcgcttgt cagaaatgat aagggagaag
     3841 aggtgattgt aattggtaag gggattgcat tcggaaagaa gaagggggat ttgattgctg
     3901 aaaatcatgt tgagaaaatc tttcggatga agaccgaaga gtccagagaa aactttatgg
     3961 ctcttctcaa agatgttccg cttgatttta tcacagtgac ctatgaaatc attgataagc
     4021 tatcaaagaa atatcattat ccgattcaag agtatctcta tgtaaccttg acagatcata
     4081 tttactgttc ttatcaagct ctaattcaag gaaggtacaa ggatagtaat ctgccagata
     4141 tttctgctaa gtatcctgtc gcttttcaaa tcgcaaatga agcttttgaa atttaccgtc
     4201 agaagctagc agatcatttt cctgaggacg aaattattcg gattgcttat catttcatta
     4261 atgctgaagg tgaaaatgaa gtggaacttg tggagtcgat tgataagagg aaagaaattc
     4321 tcaggaatgt tgaagaagtt ttaacggact atgcaattca acgaactaaa aagaataacc
     4381 atttctatga tcgctttatg atccatttga attatttctt ggattattta gacagatcta
     4441 gagatgataa ccaatcactt ctggatatgg aagatcatat taaacaatcc tatccaaaag
     4501 ccttcgagat tggttccaag atctatgatg tgattacgca acatacgggt cttgatttgt
     4561 ataaaagtga acgagtttat ctagttctac atatccaacg tttattgtca taaaaattta
     4621 tttaaaacta tataaggaga attctatcat gaatagagaa gaagtaacat tgttaggttt
     4681 tgaaatcgta gcctatgctg gcgatgctcg ttcaaaacta ttggaagcct tgaaggctgc
     4741 tgaagctggt gattttgaaa aagcggacgc tctggtagag gaagctggta gctgtattgc
     4801 agaggctcac cacgcgcaaa caagtctatt gactaaggaa gcttctggtg aggacttagc
     4861 gtatagtgtg accatgatgc atggtcaaga tcacttgatg acaactatct tgttaaaaga
     4921 cctaatgcac catttaattg aactttacaa gagaggagtt aaataaaatg gataagttaa
     4981 taatttttat tgagaaagga aagcctttct ttgagaaatt atcgagaaat atatacttaa
     5041 gagcgataaa agatggtttt atttccagta tgccagcagt attattctca agtattttta
     5101 tactaatagc tgctgtgccc aatatttttg ggtttaaatg gtctgatgaa cagttagctt
     5161 ttattttgaa accttacaac tactcaatgg ggattttagc tctcttagta gctgggacaa
     5221 ctgcaaaatc gttaacagat tcagttaata cacgtagtat ggagaaaacc aaccaaatca
     5281 actacatgtc aactttttta gcagctgttg ttggattgtt aatactggca gctgatccaa
     5341 ttgaaggtgg atttgctaat ggtcttttag gtactagagg attacttact gcatttttgg
     5401 cagcttttat tacagtaaat atctataaag tatgtattaa aaataatgta accattagac
     5461 ttcctgaaga ggttccacca aatattgcac aagtttttaa agatgttatt ccgtttgcct
     5521 tatctgttct ttcaatatat ggattggatt taattgttag aaatattttt ggtacaaatg
     5581 tagcagaatc tgttggtaaa atattagccc ctctattttc agctactgat ggttatatcg
     5641 gtttagctat tgtatttggt gcttatgctt tcttttggtt tgttggaatt catgggccat
     5701 ctgttgtaga acctttaatt gtagctatca gttatgcaaa tatagaagct aatgttcagc
     5761 ttgtgcaagc tggtatgcac gcagataaaa ttttaaatcc agttactcaa acttttgttg
     5821 taacaatggg tggtactggt gcaacactag tagttccatt tatgtttatg tggttatgta
     5881 aatcaaaaag aaatagaatt gtcggacgtg cgtctgtagt tcctactttc tttggcgtaa
     5941 atgaaccaat cctatttggt gctccaattg ttttaaatcc tatcttcttt attccgtttg
     6001 ttactgctcc tattatcaat gtttggatta tgaaattttt tgtagatgtt ctacaaatga
     6061 atagtttcag tattatttta ccttggacaa cacctgctcc aattggtatt gttatgggaa
     6121 cggcgctggc tccattgtca ttcgtcttgg ctataacttt aatcattatc gacactttaa
     6181 tctattatcc atttgttaaa gtttatgacc atcaaatttt agaagaagaa cgttcaggta
     6241 agtctaatga tgaattgaaa gaaaaagttg ctgcaaactt caacactgca aaagcggatg
     6301 ctattcttga aaaagcgggt gtcgatgcag cacaaaatac catcactgaa gaaacaaatg
     6361 tcctcgttct ctgtgcaggt ggaggaacaa gtggtctcct tgcaaatgct ttgaataagg
     6421 cagcagcaga atacaatgtc cctgtgaaag cagcagcagg cggctatggt gctcaccgtg
     6481 aaatgttacc agagtttgat cttgttatcc ttgcccctca agttgcttca aactttgaag
     6541 atatgaaagc agaaacagat aagctcggta ttaaactagc gaaaacagaa ggcgctcaat
     6601 acatcaaatt aactcgtgat ggaaaaggtg ctcttgcatt cgtacaagcg caattcgatt
     6661 aaggctagag actctgaaat agtctcctat cgttacggaa atcgctatgg cgaatttcct
     6721 attattaatt cgtcggtaaa aagatatcgt ttttacctcc tcatgtcaca attcggtgac
     6781 ttggtacaag aagtgagatg gagaaggatg gctcactgac tcctctcctc tcacttttac
     6841 tttatttaaa tcaagaaata ggtgaaaaaa atgacaaaaa cacttccaaa agactttatt
     6901 tttggtggcg caacagctgc ctatcaagca gaaggtgcta cacatactga tggtaaagga
     6961 ccagtagcgt gggataaata tcttgaagat aactactggt acactgcaga accagccagt
     7021 gatttttaca atcgatatcc agttgacctc aagctagcag aagagtatgg tgtcaatggt
     7081 attcgaattt ctattgcttg gtcacgtatt ttcccgactg gttacggcca agtaaatgct
     7141 aaaggtgttg agttttatca taatttattt gcagagtgtc acaaacgtca tgttgagccc
     7201 tttgtaactc ttcatcactt tgacacgcca gaagctctcc actcaaatgg agacttctta
     7261 aaccgtgaaa atatcgaaca ttttgtagac tacgctgcct tctgttttga agaatttcca
     7321 gaagtaaact attggacaac ctttaatgaa attgggccaa ttggtgatgg tcaatacttg
     7381 gttgggaaat tccctccagg tattcagtac gaccttgcca aagtcttcca atcgcaccac
     7441 aatatgatgg tgtctcatgc gcgtgcagtt aagctatata aagataaagg ctataaaggc
     7501 gaaattggtg ttgttcacgc tctgccaact aagtatcctt tagatcctaa aaatccgtca
     7561 gatgttcgtg cagctgagtt ggaagatatt attcacaata aatttatctt ggatgcaact
     7621 tatctaggtc gctattcagc tgaaacgatg gaaggtgtca accatatctt atcagtcaat
     7681 ggtggtagct tggatcttcg tgaagaagat tttacagcat tagaagcagc aaaagacttg
     7741 aatgatttcc ttggaatcaa ctactatatg agtgactgga tggaagcctt tgatggagaa
     7801 actgaaatta tccataatgg taagggtgag aaaggaagtt ctaaatacca aatcaaaggt
     7861 gttggtcgtc gtgtagctcc tgattatgta ccacgtacgg attgggattg gattatctac
     7921 cctcaaggtt tatatgacca aattatgcgt gtgaagaaag attatcctaa ctacaagaag
     7981 atttacatca ctgaaaatgg tctcggctat aaagatgagt tcgtcgataa cactgtttat
     8041 gatgatggac gtattgatta tgtgaaacaa cacttggagg tcttgtctga tgcgattgca
     8101 gatggagcga atgtaaaagg ttacttcatt tggtcattga tggacgtctt ttcatggtca
     8161 aatggttatg aaaaacgtta cggtctcttc tatgtagact ttgaaactca agaacgttat
     8221 ccgaagaaat cagctcactg gtacaagaaa gtagcggaaa ctcagattat agactagtag
     8281 aattagtcat tagatataga attttagtga gtcaaaaaga tgttcaaaga ttttatccaa
     8341 tctatttatg aaaaagttta tattataaat ttcgaaaaat gctctcaaat accgtgtttg
     8401 acgagtgaag aattgaaaag tctattttta aattttatca atcctgaaga atgggatact
     8461 atctcctttg attcagattt tatgccattt caacaatcgt aaccaatttc tcaaaaaagt
     8521 taaatcttat atttagtact ctgcaaaact cttatctaat cacgttgctt atactcaatg
     8581 aaaatcaaag agcaacttta aactaggaag cgagtcgcag atttctcaat gcatagcttt
     8641 gaggaattgg gcaaaaagtc tttgatatag aaaaagcata gtatcaggtg tttcaacacc
     8701 tgatactatg ctttttattg tgggaagatt tacttttttt cttctgaaat tgagttgtta
     8761 cccaggctct ttcagtttat taaggcttga tgactttaat gtgtttagat agcttaaaaa
     8821 ggattgaatc acttagttta gaatctgaaa caatagtatc aagatttgat acattataaa
     8881 aagtataaaa atcaaactta ttgaacttac tatgatctgc gagtaaatat tttttattag
     8941 aattatttaa agcgatgcgt tgagcctctc cctcttcctc gctaaaagta gctagagctc
     9001 cgttttgaat accattacag ctaacgaaag ctttagaaaa ttggagatta gagagatttt
     9061 gtagggtcaa tgtgccaaca aaagcacctg taatatcgcg ataatttcca cctatcaaaa
     9121 tcaaatctgt taattttcgt tcgcttaaaa tcagaaaaac aggtagactg ttggttacga
     9181 cgcggatatt gtcaataggc aactcacgcg caaaaaactc taatgttgtt cctggtccaa
     9241 tgaaaatagt ttctctttct tctactagac tgcctgcaaa atgggctatt tcttgttttt
     9301 ctgccgtttg gagggcttgt ttttcaatat ttgatcgctc attagtcaaa agggagttgg
     9361 ttcgaagttt ttcagctcca ccatgcacac gaatcagcaa atctttatca gctaattcct
     9421 gtaaatagcg ccttgcagtc atatctgaaa cggctatttc gtccataatc tgtttaactg
     9481 ttatggttcc tttactattt actatctcta aaattttggc taatttttct tgtttgagca
     9541 tattatcacc tcgtttccta ctactatctt accataaaca aactcatcat tcaaatacaa
     9601 aaacaacaaa atgaaacaaa aacaaaaata tcgaagtttg ttttcaaaac tttcgatatt
     9661 tttgttgggt tataactttg atgtttctag tttacttttt gatgattgag agtgatggag
     9721 aattagtcta aaccgtagtt atagtcatcg tcttgcatgg cttcaacttc gccaagaagg
     9781 taaccatttc cgacttgaga gaagaagtca tggttggaag ttcctgttga aataccgttc
     9841 ataacgattg ggttgacatc ttcagctgaa tctgggaaaa gtggatcttg tcccatgttc
     9901 atgagagctt tattggcatt gtagcgaagg aaggttttaa cctcttcagt ccaaccaaca
     9961 ccgtcataaa gactctctgt gtagccttct tcattttcat aaagagtata gagtaggtcg
    10021 tacatccatt ctttgagttt ttcttgctct tcttcaggca attcattgaa accaagttgg
    10081 aatttgtaac caatgtaggt tccgtgaaca gactcgtcac gaataatcaa tttaatgatt
    10141 tctgcaacgt tggctagttt gttgttaccg agatagtaga ggggagtgaa gaaaccagag
    10201 tagaagagga aggtttcgag gaagacgctg gcaactttct tttcaagtgg gctgccgttt
    10261 aggtagattt cgttgacaat ctcagccttc ttttgtaggt aaggattggt attggtccat
    10321 tcgaaaattt cttcaatctc agccttagta ttcaaggtag aaaagattga tgagtaagat
    10381 ttagcgtgga cagattccat aaattggatg ttattgaaaa cagcttcctc atgtggtgta
    10441 cggatgtctg cgcgaagggc ttgaacccca gtttcagatt gcatagtgtc aagaagggtt
    10501 aaaccaccaa aaacttttcc gaccaagtct ttctctttgt tagatagctt tctccagtca
    10561 tccaagtcgt ttgataaggg aatacgtgta tcgagccaaa attgctccgt cagtttttcc
    10621 caagttgatt tgtcgatgac atcttcgatg gcattccagt taatggcttt gtagtaagtt
    10681 tccatttaaa atctctttct gtgtttagta ttgcgaactc acaattattt ctactttacc
    10741 ataattctat aggagtatcg cacaaaaagt cggaagcccg acttttaaaa tgttacataa
    10801 attatgttat gacatagtag atttgatttt atcagtgctg cttagggaaa aataatgttt
    10861 ctatgctaga aactaaatca cacagctttc acattggttg gcgccgactt ctccaccgtc
    10921 atctgtaaag gtacggacgt agtagataga cttgattccc ttgttaaagg catagttacg
    10981 aaggatggac aagtcacgtg tcgtttgttt attttccctc ttccattcgt aaaggccttt
    11041 tggaatgtca ct
//
LOCUS       DAVBCG010000060        10932 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBCG010000060 DAVBCG010000000
VERSION     DAVBCG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.060"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..605)
                     /locus_tag="VQ495_001274"
     CDS             complement(<1..605)
                     /locus_tag="VQ495_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2531229.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCR"
     gene            1229..1411
                     /locus_tag="VQ495_001275"
     CDS             1229..1411
                     /locus_tag="VQ495_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2531230.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1421..1786
                     /locus_tag="VQ495_001276"
     CDS             1421..1786
                     /locus_tag="VQ495_001276"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2531231.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            1783..2973
                     /locus_tag="VQ495_001277"
     CDS             1783..2973
                     /locus_tag="VQ495_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2531232.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(3488..4480)
                     /locus_tag="VQ495_001278"
     CDS             complement(3488..4480)
                     /locus_tag="VQ495_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531233.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            4640..6394
                     /locus_tag="VQ495_001279"
     CDS             4640..6394
                     /locus_tag="VQ495_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531234.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            6378..8099
                     /locus_tag="VQ495_001280"
     CDS             6378..8099
                     /locus_tag="VQ495_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531235.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            8110..8739
                     /locus_tag="VQ495_001281"
     CDS             8110..8739
                     /locus_tag="VQ495_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2531236.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            8640..9197
                     /locus_tag="VQ495_001282"
     CDS             8640..9197
                     /locus_tag="VQ495_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2531237.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            9185..10549
                     /locus_tag="VQ495_001283"
     CDS             9185..10549
                     /locus_tag="VQ495_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531238.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         4030 a         1516 c         1852 g         3534 t
ORIGIN      
        1 caacggcatg actgagaaca cccaccacgg ttggcatcac gcatactcat gtggtttgaa
       61 agagtacaac gtccagagta tgaaatacac atagctccat ggacaaaggc ttcaatttca
      121 acatctgtac gtttgcggat ttcagctaat tctttcattg aaacctcacg tgctaaaacg
      181 acacgagtca agcctagctc tttccagaac tcaagggttt catagttagt ggcactggct
      241 tgggtagaaa ggtggatttc aaggcctggt gcttcagtcg ctgcaatcat aatcaaggct
      301 gggtcagata cgataactgc tgcaatcccg atatcacgca gtttacgaaa ccactcacca
      361 gcaccagctt catttccttc gtgcataacc atattagccg ctacatagac cttggcacca
      421 tacttggccg caaactgcac gccttcttcc atctgttcga aagtaaagtt tcccgcacgg
      481 ctacgaagac cataggcctg accaccgata aagacagcat ctgctccata ctgaacagct
      541 acctttagct tctctaaagt ccctgcaggt gataaaacct caggacgttt taatgttttt
      601 gtcatgattt ctcctatttg caatataaaa gtttgacatt tttccttctc attttatagt
      661 aaataaagga taaaatcaag cctagacgga ttgttttgac aattctaatc ttttagactt
      721 tgcataataa ctattaaaca gtcgaaaatt accccagttt taacaagtag tcaaaaagtc
      781 taaaaaagta aaaaccagta tcccaagata ctgactcgta aaaatatata aattagtcct
      841 tagatgacta ctcccactct gttgctccta taatggaaaa ggattgatac agtctagaat
      901 atcgcttttt tatttattat tctgatataa aactgttaga attgattttt tgttttatac
      961 ccaaatatga atagagacgt agctaataag ttgaataaca tatttggaaa aatgagaaat
     1021 agcaatatta ctagaacttt tagaaacaat caatatttaa aaagaaaaaa gctcaaagaa
     1081 tttattaaac catgttataa tagatatagc tataatttta tttataacaa gaggatagaa
     1141 atgaccgaat tagaaagaaa aaatcgaaaa attagctaag aaatattctg ataacttaaa
     1201 catcaaagtt caagagagag ttcgtgaaat ggcaaatgat aataagagcc attatttgat
     1261 atacagagtt ttaggtattt catttgaaga aggagaaaat atcgatttgt atcaaaataa
     1321 aggtcgtttt ttatacaaat atgctggttc atttttagaa gaagctgcag tactatcctt
     1381 taacgaaaaa tttggtacag aaaatactta aaaagttaac attcctaatt ctgaaaatac
     1441 aaaacctaag acttttgaaa ttgattgttt agtcggagaa aaacacgcat acgaaataaa
     1501 atggtgggat gcaactacag atggagacca tataactaaa gaacacacta gaataaaagt
     1561 tattcataac aaaggatata taccaattcg gttaatgttc tactatccaa atagaactca
     1621 agctataaaa attcagcaaa ctttagaaac attgtataac ggtattggag ggaaatatta
     1681 ttatggagat tctgcctggg aacatttaag agcagtgacc ggtattgatt tacttagtat
     1741 tctaacagat attgcaaata aaaaaacagg ggtaaaatca aaatgacagt attaaaagga
     1801 gataacttag aaatattaaa aactattgaa tcctcaagta ttgatttaat ctatatggac
     1861 cctcctttct ttacacagaa aacccaaaaa ttatctaata acaaaaatat tatgtattca
     1921 ttcgaagata cgtggacttc gattgaggat tacaaagaat ttttgtctgt aagattagaa
     1981 gaatgcaaaa gagtgctaaa aaatagtggc agtattttcg ttcattgtga taaaattgca
     2041 aatcatcata ttagattaat tttagataat atctttggag cagatatgtt tcaaagcgaa
     2101 attatatgga actataaacg gtggtctaat tcaaaaaagg gattattgaa caatcatcaa
     2161 aacatttact tttattcaaa gtcaaaagat tttaaattta atacaatttt tacagagtat
     2221 tcttctacta caaatatcga ccaaatacta gtagaacgaa aacgaaatgg aaactctaaa
     2281 actatatata aagttgataa taatggtaac tatattctag caaaagagaa aaatggagtt
     2341 cccctttcag atgtttggaa tataccattt cttaatccaa aagctaaaga aagagtaggt
     2401 tatcctacac aaaaacctat tctgttatta gaacaaatta taaagattgc tactgataaa
     2461 aatgatatag ttttagaccc gttctgtgga agtggaacta ctttagtagc ctccaagatt
     2521 ttgaatagaa attatatggg gattgattta tctgaggaag ctatcaatat aactcagcaa
     2581 cgtctggaaa atgttataaa aacaagttca aatttattga ataaaggaat cgaagcatat
     2641 agaaccaaaa ctgaggaaga ggaaaacatt cttaaattat tacaggcaaa aattgttcaa
     2701 agaaataaag gaattgatgg ttttttacct aaacattttc aaaaaaaacc gatacctata
     2761 aaaattcaaa aaaataatga atgtctgaat gagagtatct ctttattaca gaatgctata
     2821 aactccaaaa aacttgattt tggagtagtt ataaaaactc attcagataa tttattattt
     2881 gattttgata cgattcctga aaatataata gtagttgacc attttgaact tacaatcgaa
     2941 aaatggttaa gtaaaagtca acagttacta tagtttgcaa attatttaca gaaagaacaa
     3001 aaaatgctct gaaacactga tttcaaggca ttttttgtta gtttaaaatt actcccattc
     3061 ttcttttcca aacgtacaat atatccaaaa ccattcaaaa tactagattc tattttttat
     3121 aatatcacta aatccaccta attataggac gttttcagat ttttagtccc gtcccagtcc
     3181 cagtaccgga gaaatattgt tttaatataa tatctctttt tgtcttctaa gctcttaaaa
     3241 gcaaaagaac aagtaaagag tcaagacaag gataaaaagt ccatattagg gcaaataaaa
     3301 agctttaaga cagatgacaa atctaagtca aataagaaag accatagcaa aggtgcagag
     3361 agataaatat tggcggtctt cggactgcct ttattttttt atccattttt caaatcaaat
     3421 ttattcagac tatatatgca catatacact taaattcata taaaaacatg gcttgtaaaa
     3481 aattacttta atcacaataa tcgcatttaa aattgtgatg tttgcaagct aaattacgga
     3541 cttcacttgg aagttttccc ttgtatcttt tataatagat agaaaatttg ctggcagatg
     3601 aatatccaac agattctgct atctctttta taggtagttc agtgtttaaa agaagagttt
     3661 cagctacatt cattcttttt ctttgagtgt actctgtaat gctttgacaa tatttttcct
     3721 taaataaatt ttttaattta gtaccactca ttttagatat tttttcaagc gtgccttgat
     3781 ttacattcgt tgcaaaatga tcatctaaga atcttgctac atcttcaagt gctttatcat
     3841 catcaatttc aattttatat ttttttctat ttaagtatgt gtcaattact atacttatcc
     3901 attcatttgc ctttgcttta aagaaaaaat cagcggcagg agcgtccatc ttacaattta
     3961 atatttccat tgccactctt tctaaggcct ttgtaagtat tatttgattc ggttgaagca
     4021 aggttgaata aaaagattct ggattaatgt taatagatgc taaatgtttt tctattagct
     4081 cttttttaaa accaatggaa acagcaagat aacaacaatt ctcgtgtaat aaaaaaacaa
     4141 aatcatcttt tatattatca aaatcaaaag tacataaaga gtttgcggta atagtttgat
     4201 acggattaaa cttttctccg tttgcactga caatgtaact tgaataaatt gaaacatagt
     4261 ctgacatact ataagtgcta ttttgaacta cttcctcttt gatataaaaa tcatgtatat
     4321 cgataatgaa gctgcctcct tcataaaacc aatataggcc ttctgcataa gttgaattct
     4381 cttttttcca acaaaatgta tgacctgcac ttgaatactt ctcattgttt ttacattcat
     4441 ctactttcat ataatctttt acaaaatcat aatatgacat aacacactat cccttttaga
     4501 caatattcca attagcctta ttaattcaaa actattgtat tagtaattat aacagatgta
     4561 taatagaaaa gcaatgatag atattatcaa ttaagcgaat ttatatctaa aagggatatt
     4621 aaagaaagga gatatgctta tgaagattta caaaaaacta tttgcttatg tccaagataa
     4681 gaaatatctt ggggttttgg ccataatttt ttctgctata tctgctgtac ttacagtata
     4741 tggatattat ttaatctaca aatttctaga taagttaata attaattcaa acttatccgg
     4801 tgcagagagt atagcattaa aatctgttat tacactaaca agtggagcga tattttattt
     4861 tgtctcagga atgttttcac atatcttggg atttaggctt gaaacaaatt taagaaaaag
     4921 gggaatcgat ggtctggaaa aagcaagttt taggttcttt gacttaaatc catctggtca
     4981 aataagaaag atcatagatg ataatgcagc acaaactcat caggtggtag ctcacatgat
     5041 tccagatagc tctcaggcaa tagtcacacc tatacttgtc cttgtacttg gctttatagt
     5101 aagtataaga gttggcataa ttttgcttgc tcttactata attggtggct taattttagg
     5161 ggcaatgatg ggcgagcaag aatttatgaa gatataccaa gaatccctat ctaaactaag
     5221 tgctgaaact gttgagtacg tgagaggaat gcaagttgta aaaatattta aagcaaatgt
     5281 agagtctttt aaaagctttt ataaggcgat aaaagattac tcaaagtatg cttatgatta
     5341 ttccctatct tgtaaaaggc cttatgtttt gtatcaatgg ttattttttg gactgattgc
     5401 aattttaatt attcctatag tttattttat gactagctta gctagcgcag aggtgatttt
     5461 acttgagctt atcatgattt tatttttatc aggagttctc tttgtttcat tcatgagaat
     5521 gatgtggtac tccatgtata tttctcaagg aaattatgca gtagatactt tagaggcgct
     5581 ttacgaagat atgcaaaaag acaaattagt gcatggtaat gtcaataatt ttaaaaacta
     5641 taatatagaa tttgagaatg ttagctttgc ttataatgat aaagctatca ttgaaaattt
     5701 atcctttaat ttagaagaag gaaagtccta cgcacttgtc ggttcatctg gatcaggcaa
     5761 atcaacagta gcaaaactta tatcaggttt ttacaatgtt aataaaggaa gcataaagat
     5821 aggcgggata gcaataagtg aatattctga cgaagcctta attaaagcca tttcctttgt
     5881 ttttcaagat tcaaaattat tcaagaagag catttatgat aatgtagctt tagctaataa
     5941 agatgcgacg aaagatgacg ttatgagagc cttaaaatta gcaggatgcg atttaatatt
     6001 agacaaattc ccagaaagag aaaatacaat cataggctca aaaggtgttt atttatccgg
     6061 tggagaaaaa caaagaattg caattgctag agcaatttta aaggattcca aaattattat
     6121 tatggatgaa gcatcagcat ctattgaccc agataacgag tttgaattgc aaaaagcttt
     6181 taaaaatctt atgaaggata aaacagttat catgattgca cacaggctat ctacaattaa
     6241 agaccttgat gaaattattg tcatggatag tggaaaaatt atagaaagag ggtctgacaa
     6301 agaattaatg tcaaaagata caaggtataa gagcctgcaa gagatgttta acagtgcgaa
     6361 tgaatggagg gtttcaaatg aaagagtttt ataaaaaaag atttgctctt acagatggag
     6421 gagcaagaaa tttaagtaaa gcaacactgg cttcattttt cgtttattgt ataaacatgc
     6481 ttcctgccat attacttatg atttttgctc aggaagtttt ggaaaatatg ggcaaaagca
     6541 atggctttta tatagtattc tcagttttga ctttgatagc aatgtatatt ttgctttcta
     6601 tcgaatacga taaattatat aacacaacct atcaagaaag tgcagattta agaataagga
     6661 cagcggagaa tttatcaaaa ttacctctat cttacttttc taaacatgac atttccgaca
     6721 tttcacaaac aatcatggct gatattgaag gcatagagca tgcaatgagc cactcaatac
     6781 caaaggtggg cggcatggta ctgtttttcc cattaatatc tgtaatgatg ctagcgggca
     6841 atgtcaagat gggtttagct gtaattattc catctatttt aagctttata tttatacctt
     6901 tatctaaaaa atatcaggtt aatggacaga atagatatta tgatgtctta agaaaaaact
     6961 cagaaagctt tcaagaaaat atcgaaatgc aaatggagat taaagcatat aatttatcga
     7021 aggatattaa agatgactta tataaaaaaa tggaagatag tgagagaata cacttaaagg
     7081 cggaagtaac tacaatttta actttgtcta tatcttcaat atttagcttt atatctcttg
     7141 ctgttgtgat atttgtcggc gtaaatctaa ttattaataa agagataaat tctctctacc
     7201 ttataggata tttactagct gctatgaaga taaaagactc tttagatgca tctaaagagg
     7261 gcttgatgga aatattttat ttatcgccca aaatagaaag attaaaagaa attcaaaatc
     7321 aagatttaca agaaggcgat gactatagct taaaaaaatt tgatattgat ctaaaagatg
     7381 ttgagtttgc ctacaataaa gacgcaaaag ttttaaatgg tgtaagtttt aaagctaagc
     7441 agggagaggt cactgctttg gtaggtgcaa gtggctgcgg taaaacaact atcttgaaac
     7501 ttatatcaag actttatgat tatgacaagg gacaaatctt aatcgatggc aaagatataa
     7561 aagaaatatc aacagaatct ctttttgata aggtctctat agttttccaa gatgtggttc
     7621 tctttaatca aagcgttatg gaaaatatta gaatcggtaa gcaagatgca agtgacgaag
     7681 aggttaaaag agcagcaaaa cttgcaaatt gcacagattt tatagaaaaa atggataaag
     7741 gtttcgatac agttattggt gaaaacggag ctgagctatc aggaggagaa agacaaagat
     7801 tatcaatagc cagagccttc ttaaaagatg cgccgatatt gatcttagat gagataacag
     7861 caagccttga tgttaacaac gagaaaaaga ttcaagagtc tttaaataat ttagttaaag
     7921 ataaaactgt tgtaatcatt tcacatagaa tgaaatccat agaaaatgca gacaagatag
     7981 tagttcttca aaacggaaga gtagaaagcg aaggtaagca tgaagagctt ttacaaaaat
     8041 caaaaattta caaaaattta atagaaaaga caaaaatggc agaagaattt atttattagg
     8101 aggactacaa tggataataa aaaattaaaa gtaaaagatt tagtaagtat cggtgttttt
     8161 ggcgtaattt attttgcctt catgtttgga gttggtatga tgggcttgat tccaatattg
     8221 ttcttaatat acccgacagt attagccata gttgcaggaa ctgttgttat gttatttatg
     8281 gctaaggttc aaaagccatg ggcactattt atatttggta tgatatcacc acttgtgatg
     8341 tttgcagctg gtcataccta cgtagttgtg gttttatcac ttatagtaat gataatagca
     8401 gaattaatta gaaagattgg taattataat tcatttaaat acaatatgct ttcttatgca
     8461 atcttcagca catggatatg tagctcttta atgcaaatgc ttttagcaaa agaaaaatat
     8521 atggagtggt ctttgatgac gatgggaaaa gattatgttg atgtattaga aaagttaata
     8581 acttatcctc acatggcttt aatagcctta ggtgctttct taggaggaat tcttggagca
     8641 tatataggca aggctctatt gaaaaaacac ttttcaaatg gattatattg tgtgggatac
     8701 tttactcctt gcctaatttt atggtgttat ctgaattaaa ccctatagtt aagatgtttt
     8761 tgagtatacc tattgttatt agaatgttta ttttaccatt tatggcagca agctttatga
     8821 taaagacctc ggatgtaggc gcaataattt catcgatgga taagcttaag atttcaaaga
     8881 atgtatccat acctattgcg gttatgttta gattcttccc atcttttaag gaggagaaga
     8941 aaaacatcaa aatggctatg agagtaagag ggataaattt taaaaaccca gtcaaatatc
     9001 ttgaatatgt ttctgtgcca ctactcatta tatcatctaa tatatcagat gacattgcaa
     9061 aagcggcaga aacaaaggca atagaaaatc caattgccaa gaccagatac attcgcgtaa
     9121 agatacagct aattgatttt gtttatgttt tagcggttgc tggacttatt gtgggaggct
     9181 taatatggtt gaaataaaaa atttaagtct tgattatggt gaagagcata tattagatga
     9241 tatatcacta tccatagccg agggagagtg cgtgctattt acaggaaaaa gtggaagtgg
     9301 taagtcatct ttaataaatt caatcaatgg actagctgta aggtatgata acgcaaagac
     9361 aaagggcgaa ataattattg atggtaagaa tataaaaaat ttggaacttt atcaaatctc
     9421 aatgcttgtt tcaactgttt ttcaaaatcc taagacatat ttttttaatg tcaatacgac
     9481 attagaatta ttattttatt tggaaaatat cggtcttgca agagaagaga tggacaggcg
     9541 tttgaaggat atacttgaga tattcccgat aaaaaatctt ttgaacagaa atatatttaa
     9601 tctatccggc ggtgaaaaac aaattctttg cattgcagct tcttatatag caggtacaaa
     9661 gattatagtt atggatgagt cttcatcgaa tttagatatt aaaagcataa gtgttttggc
     9721 aaagatgcta aagatattaa aagagaaagg cataagcata attgttgcag agcatagaat
     9781 ttattatttg atggacatag ttgaccgtgt atttttaata gataaaggaa agcttaaaaa
     9841 aacttatact agaagtgaat ttttaaagct agataaaaat gaattaaatg ctttaagttt
     9901 aagagataaa gaattaagta aattaaaagt tccttattta aaagaaggtg gagagtatca
     9961 gataaaaaat cttagctaca aatttactga tgatgagtgt ttaagcttaa aagatatttc
    10021 gttcaagctt gggaaaattt atggcataat aggatccaac ggacgaggaa aatcaacgct
    10081 tttaagatgt ttaataggtc ttgagaaaaa atcaaaagaa gaaatttatt ttaagggaga
    10141 gaagctatct aaaaaagaaa gactcaaaaa ctcttcactt gttatgcaag atgtaaatca
    10201 tcaattattc acagatgaag tattcaacga gcttagatta ggagtaaaga attttgatga
    10261 agaaaaggcg aaaatcattt taaaagattt aggcctggac gaatttattg aaaggcatcc
    10321 gatgagttta tcaggagggc aaaagcaaag gcttgcaata gcatctgtta tgtgcaagac
    10381 ttctccattt atcttttttg acgaaccttc aagtggtata gattattcca atatgataaa
    10441 aatatctgaa ctgattaata agtataaaac catggataaa ataattttta ttgtttccca
    10501 tgatatagaa tttttaaatg aagtggcaga tgaaattttt gaattgtaaa atagagtctg
    10561 ttaaacatgt ctatagaact ggtaaattgt ctaaaaattt aaaagatact aacacatatt
    10621 gatatttatt agaaaattaa cttcaggatt tttacagtat ttataaactt tataatatgt
    10681 atttatagta ttaataattt ccatatcttt agggaaatta tttatttact taccataatt
    10741 aaagagatga gagataaata tcggcagtct tcggactgcc tttatttttt tacttaattt
    10801 aatgcagtaa atcatacgat tgaaaggaat ctgcgttata tgctataatt aggtagttaa
    10861 tctatacatt tagaaaggta aaatatagaa ttggagaatg atatgggatt ttcatataat
    10921 aaattgtaac ca
//
LOCUS       DAVBCG010000061        10931 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBCG010000061 DAVBCG010000000
VERSION     DAVBCG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.061"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..350
                     /locus_tag="VQ495_001284"
     CDS             <1..350
                     /locus_tag="VQ495_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2531239.1"
                     /translation="DYINCLSETADKNWHKSDRIFVTNTGKTVHSSILSKSLQRANER
                     LKKPIPKHLSPHIFIHITISILSENKIPLKTITDRVGHPDSEVTNSIYTHVTKNMKDE
                     AINVLDKVMKKIF"
     gene            complement(842..1252)
                     /locus_tag="VQ495_001285"
     CDS             complement(842..1252)
                     /locus_tag="VQ495_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531240.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1270..1647)
                     /locus_tag="VQ495_001286"
     CDS             complement(1270..1647)
                     /locus_tag="VQ495_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531241.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1589..2017)
                     /locus_tag="VQ495_001287"
     CDS             complement(1589..2017)
                     /locus_tag="VQ495_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET2531242.1"
                     /translation="MAGNENDGLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRLKQDEMMDKTTQMLYLASSNAVSVLNDIMMDAKINPFIRTQAAKTILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGDQRVTRTQGIIK"
     gene            complement(2061..2666)
                     /locus_tag="VQ495_001288"
     CDS             complement(2061..2666)
                     /locus_tag="VQ495_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531243.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(2756..2929)
                     /locus_tag="VQ495_001289"
     CDS             complement(2756..2929)
                     /locus_tag="VQ495_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531244.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3528..4424)
                     /locus_tag="VQ495_001290"
     CDS             complement(3528..4424)
                     /locus_tag="VQ495_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2531245.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4436..5245)
                     /locus_tag="VQ495_001291"
     CDS             complement(4436..5245)
                     /locus_tag="VQ495_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2531246.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5320..5535)
                     /locus_tag="VQ495_001292"
     CDS             complement(5320..5535)
                     /locus_tag="VQ495_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531247.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5537..5737)
                     /locus_tag="VQ495_001293"
     CDS             complement(5537..5737)
                     /locus_tag="VQ495_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531248.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(5734..5925)
                     /locus_tag="VQ495_001294"
                     /pseudo
     CDS             complement(5734..5925)
                     /locus_tag="VQ495_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5914..6204)
                     /locus_tag="VQ495_001295"
                     /pseudo
     CDS             complement(5914..6204)
                     /locus_tag="VQ495_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6374..6526)
                     /locus_tag="VQ495_001296"
     CDS             complement(6374..6526)
                     /locus_tag="VQ495_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531249.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6537..6803)
                     /locus_tag="VQ495_001297"
     CDS             complement(6537..6803)
                     /locus_tag="VQ495_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531250.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(7014..7196)
                     /locus_tag="VQ495_001298"
     CDS             complement(7014..7196)
                     /locus_tag="VQ495_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2531251.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7258..7761
                     /locus_tag="VQ495_001299"
     CDS             7258..7761
                     /locus_tag="VQ495_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531252.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            7835..8998
                     /locus_tag="VQ495_001300"
     CDS             7835..8998
                     /locus_tag="VQ495_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2531253.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9152..10456)
                     /gene="eno"
                     /locus_tag="VQ495_001301"
     CDS             complement(9152..10456)
                     /gene="eno"
                     /locus_tag="VQ495_001301"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2531254.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10620..>10931
                     /locus_tag="VQ495_001302"
     CDS             10620..>10931
                     /locus_tag="VQ495_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HET2531255.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SP"
BASE COUNT         3196 a         2170 c         1808 g         3757 t
ORIGIN      
        1 ttgactatat aaattgtcta agcgaaacag ccgataagaa ttggcacaaa agcgaccgta
       61 tttttgttac caatacagga aaaacagttc atagttctat cttgagcaag tctctccagc
      121 gagcaaacga acgccttaaa aaaccaattc ccaaacatct gtcccctcac atcttcatac
      181 acatcactat tagcatctta tcagaaaata aaattccttt aaaaacaatc acggacaggg
      241 ttggtcatcc cgactctgaa gtcactaatt ccatctacac ccacgtcaca aagaacatga
      301 aagatgaagc aatcaatgta ctggataaag ttatgaaaaa gattttttaa aaagttttgt
      361 cccttttttg ccctctaaat acaaaaatag cccttcggat aaaatccgag gggctagaaa
      421 cgttgttaaa tcaacggccg aacttttgaa tttcatggtt cgggataaaa tagttcactg
      481 aactatttta ttttttaagg ttatcataat atcaaatagt tcaattaaat acgctaaatt
      541 actaatatac tttttacctt tttcattcta aaatgtaaag tacaaacaat tacaatatac
      601 tagaggggga gtaaaaaagg taataaaatt gacgagttca gcaggcaagg gaaatagcac
      661 ttttcatggt gttatttttt taattcagca atggagtagc cgcttaattg atagaaacaa
      721 aaagcaccta gtttcctaga tgctagcaca atagcccgga ttcgaaccgg aatctcctca
      781 attaaggtgc aatccctata tactactcat tgtgctttgg ttcattatat catacaatca
      841 actagctatc taccaactca tgatacgctc ccatattcgg taatggaata atactaaaat
      901 acaaatctga gtaagtttca aaccatttat tcagcttgtt ataggctggc gactgactaa
      961 caaataatat ccgttgacaa gctccgataa cattcatgtg attatacaca tagacctctt
     1021 taatggccgt taaaatatcg tcttcacttg tctgtaccct aatatctgtt atctgcttaa
     1081 tcgtagcgta ttttttataa gctagcatat ctttattttt agcagcatca aacattttac
     1141 actcaagaac actatactta ggtttttctt tatctctcat gaaataccac ttgagccata
     1201 aaattttttc tctgtgaatt actgaaatac gttcgatttt tttctttggc atgattacct
     1261 cctaaattat taatctaata gctttaacca ctcacttttt gagattgttg tataaggttc
     1321 atctagcgcg tgatataaag gttctaaacc tttgttaaat cggatatagt ccattttatc
     1381 ctcatgcttt ggaaaatata agattcctgt ttcatattca tttagaccac ggttacacca
     1441 atcatcaacg ataacctgca cttcccattc acgccacttt ttaaggtttt ctaaaagttc
     1501 aatatcacta aataactcac catcaacttg gaaaaattct cccagatcat cgttaccctc
     1561 gacgatgata acttcaggcg tatttctact acttaataat tccttgagtt cttgtaactc
     1621 tttgatctcc tttagatact tcctcaattt ctaacctcaa ttcttcaatt tgtcttacta
     1681 ctccaaaaat ttcatgagtt ttatatgatt gttcaagtat agtttttgct gcttgagttc
     1741 ttataaaagg gttaattttg gcatccatca taatatcatt gagtacagaa acagcattgg
     1801 atgaggctag atatagcatc tgtgtagttt tatccatcat ctcatcttgt tttagtctca
     1861 atgtcttttt aacagctgca acttttagat acttatgccc tgttgccctt gacacccctg
     1921 ctttttggca cgctttatct atcgttggct cagtaagcat ggcatctatg aattttattt
     1981 gtttagatgt aaggccatca ttttcatttc ctgccatcta ttacctcctc gttatcaaaa
     2041 taaaggggtt gctagcccct ctatttgttt aaatactcat ccaaacgacg gtcaagctca
     2101 ggggcatctg aatttactac catttcagtt gttacagctt ggattttatc gccgttgata
     2161 agcaaactgt aatgacaata tgaaagttgc cgcttaaaga ataagattag actttctagc
     2221 gcctgctctg cattgaataa ttccaagttg atagcaattt ctggatattg ttcagcaatt
     2281 gaccaaagtt ttttaatagc aagaggtttg ttcttgtatt tttcaaaata acctagcaat
     2341 tcatcctttg tcatcttcat actagctaac aaggtcagct ctgccacgtc atcagctgtt
     2401 acgctgagtt ctttttcttt taatgcctct agctctagat catgctgttt ttcaatttct
     2461 gcatgaattt tattataggt gtccctagaa taagtgtccc cctctaactt ataagcctct
     2521 agctggtctt tagcgtattt ttcgcctatc ataccatttt gtaactgttt atgtaattcc
     2581 tcttgtttag caatgtagcc agtcatgtac ccatgctttt tcttgccaaa agccttaata
     2641 tcatccttga ttgttgtaaa tgtcatgtta tcctccttac gaaaaaataa aagaggtatg
     2701 acaaaagagc gttaaactca attatcatac ctctggtttt tccagtcagt atttattaac
     2761 attgttctcc agtggtcact tccttataaa ctaactgccc atcttgagct tttagcttaa
     2821 tgctaccatg atctggaatt tcaatgtact ttattatacc attttttgag tataaaacaa
     2881 agcctttctc catcatttct aataatttat tatccttgtt ttcagtcatg aatttctcct
     2941 ttatttcaag tgtcccaacg ggtacggttg taagctgaga cattttaggg ctatctttga
     3001 gcgacaaaaa tgtcgaaaag tcttcagttc ctgtataacc gttattagtt ttgtttgcta
     3061 ctcatttttg agtcacaaat ctgttaagca aaaatgagta atagctcgta agataagtaa
     3121 atctaatact tgtattccta aagcgttgat ataactgata ttttcaacta tccaacgctc
     3181 tttactaccc tttttagtgt gctttagctc atttttgagc ttttttgagc ttttttgagt
     3241 tccattttgg gaatgcaaag caggcgcgca ccgtgcatgc ttttttcttg ctcatagcct
     3301 caccctcaga agtttgccga ccgagagcgt ggggcttttt atttttgtat aattaaaaac
     3361 agctgcacaa tttttgtaca gctacgaccg aggcaggggc gacctgccgt actttacact
     3421 agataatatc atctaggtag tccaactgta ttttatcata ttaaaggcta cattacaatt
     3481 tctcaccctg tgaagagtgt ctatttttgt ttaggtttgt aagtcgatta cttcaactgc
     3541 tttaccttat tttttaactg attaattgag tatcgcttat ctttgattgt gaaagcctta
     3601 aaagtgttac cctcaagacc ttttagaata cggctataat tccgctcgtt gtatactatc
     3661 ttgagttcat cgctatcaag atttgtatta aatatagtat tttctcgatt attcagtaca
     3721 tcaaaaagta aatcctgctc ccaatcgctc ttaggtttaa taacagcatt ttttgcacct
     3781 aaatcatcaa taattaagta atcaacagac ttcatgagtt cagtagcttc aaactctgta
     3841 agtgttgcac ctttaccata attccacccc tctttaattt gtttgatcat ttcggttagg
     3901 cttacaaaaa gcacactctt aggttctcct tttgtcttat acccctcatt tatacctttg
     3961 gcaatagcaa ctgataaaag tgtttttcca atccctgtac ctcctgtgat aagcgtattt
     4021 cccctcatgc catcaagata tttttgtacc tgaccttttg caaattctaa aaatcgcttt
     4081 tcttctgatg ttacagcatt aaaatcatca aaagttttag ttttaaactc atctgctaca
     4141 tagctcttat tgctcatcaa cacattataa gtttgcagat atagtttagc attcaaatta
     4201 tcagcaatcg catcttcttc atcttgcttt ttctgttctt cttggcattg ttcacaatag
     4261 ggtgggatac agcgaacttc ttttattacc tctccgttct cattccaccc cactactaca
     4321 tgtctttctc ctttgatttg tgttagctgt atttcatgct taggacacaa ttcgtctagt
     4381 ttaaatgtct caatatttcc taaactagat tgtaatgatt tcattttctg acctcctaaa
     4441 atggtttttc ttgtgttggt atccaatctt catagctggt aggctctagt tgattggttt
     4501 gctgtttttt agcctcacgc gctgccctgc tatttctaac aagttccacc gtcaataaat
     4561 tgtcctgttt ccaacggtta aggattgcct tgatgtatgc aaagtttgct ttaccctgac
     4621 tgacagcctc ttttaacgcc tcatggataa gctctgggct aaaatcttct agcatatact
     4681 gcaattcttg aatctgtaac ggtgacaatg ctttacctgt ctcagcttca aaagatttat
     4741 agagatttac aaaatcttga ttgatggtag agggagtggg tgattgttct ttatctactt
     4801 ctatctctaa ttctttctct atctctgttg gacatgagtt ggaaaatgtc aaagatggtt
     4861 ggacttcttc caattttcct ttttttcgtt gttcacgttt ataactcgcc cagtttgttt
     4921 cactcataac catagctttt gcctgtggta gttctgcgtt tttatcttcg tcaatctgta
     4981 ttaaaccgca ttttgtaaag tatgccatgg tcatatctat atcatcttct gatacatcta
     5041 attttagggc taactcttcc ttgagattgt taaagtagcc ctcatagtat aaaatacagt
     5101 cactttccaa gctctctaac atgagcctaa tataaataac tgtcatagtg tacccaccag
     5161 gtaccgtctt tagtctcttg ataaaaatat tatcaaaaaa tttcttatca atttttagcc
     5221 aaaaatagac ttttgttttt gccatcgtcc tctcccttgc tcatttataa ttctctacct
     5281 tgattagtct taatactcgc ttgtcattca ttgttagact cattcaattc ttccccatac
     5341 gtctacccct gcaagctgga tatatcgccc ataatcaggg tttaaatcct cgctaggtgt
     5401 ttctatcgtc tgttggtttt ctcgctccag ttgggcgctt tttttgcggt ctcggtggtt
     5461 taaataaagc agtaagccaa tcaaaatcac ggtaacaaaa accgcctgtg tattgcttat
     5521 atctagttca ttcatgttat gccctcgctt tgtaattctt gatgtatgcc acttgttcag
     5581 ctcgcttcat attcaacaag tcgtctattt ccacactggt tactttttta tttacaaaat
     5641 cagaaatcag ttgaaaaatg tttggacttt gtagctggat ttcagccatt gcctcatcaa
     5701 attctgcttg tgtcatgttg tctaaatcta gtgtcattgc attgcctcct caaacttctc
     5761 tataagacaa cttttatttg ctttctgagt tccattttta gagttaaaaa gaatatattt
     5821 taaggttaca gtagcctcta aatactcctt ttcagcatgc tctatatacg cctgttgctc
     5881 tgcttcgttc tcaaaaaagt gcttagctta gcgtttaaag aatgctttga ctaggtttag
     5941 tttgaattgc cgtactgctt cggtattctt tagataagtg atcagcaaag tagcctgttg
     6001 ctcgttcaga atataggatt ttttaggttg tcctctagtg tctaatttat ggattttaaa
     6061 tccaagtatt cccaactctt caaagtcaac cttattttct cttattaagc gtgtgatagt
     6121 gtggtgttgt acgtcagcac attcagcgat gatctcgctt gtggtatacg gctctttctt
     6181 gccgtccatg tagactagtt ccattatggt tctacctcct gtataaattt agctagctta
     6241 ctttttaatt gtctcctcta gcccttttta gcctctacaa cggttttggc taacgtgtta
     6301 gaggctttta taacggcctg tatagctgtc tctatttcct ccgtggaata gtagctttgt
     6361 tttctaatat cattcaagac ttgtttaacc catttcttga aataaataaa attacatctt
     6421 ctgtactctt ggcatctgct ttatctgaga caaatagaat gtcaatgcct ggtagataaa
     6481 acccaccgtc tatttgttta gttggtttga aagtaactat tttcatttca taactcctat
     6541 tttattgcta aaaataaata cacatctgac agacgataga atactttctt agctccatcc
     6601 atcggggtct gatatcgttt aagagcaaga tcctcccagt tatcaagggt tgtagcactt
     6661 atacctagca gatccattaa ttctttacga gaaattaaat ctaacttact agatcgttgt
     6721 ttttctagct caactaaagt ttcaagagtc tgtttaagaa gctgtaaacc ctgtaactgt
     6781 atttctttag acagaatttc catttatatc ctccattttc ctttttataa attaagttgt
     6841 ctttttcctc taattttgat aaaataagaa gagattattt agcaaagggg gttgtccctt
     6901 tgctaaatac ctaacaaaac cacgcgcctt gcctttgctt gagttttgtt atttttcctt
     6961 agccgtgtac gttggtttgg tcgcctgtac atggcttttt acttgcgtta aatttattct
     7021 actagccaag tcatcacgct ctcatagata cgcttagagg catcatagtt tccattctca
     7081 acttttgtta gtgtttgaga tttaataccc aactgctccg atactgcttt tttggttagc
     7141 tgtaatcttg cacgcttgat acgtactttt tcagcaagtg atagtgtaat caacattggt
     7201 ttcacctccc ttttttctcc atttttggca ttttgtgtta taatctcttt agaaaggatg
     7261 tataagatga atagaattaa gcaattacga aaagaaaata atcttactca aagagaactt
     7321 gctaatgaga ctaaaatccc ttatagaacc atacaacgtt gggagaatgg ggaaactgat
     7381 ataaaatccg atgctgctca ggtacttgct gactattttg gagtatcaat tcctcactta
     7441 ctaggttata gcgatttgat agcagataca gatagtgata ttaaagcaat ggcatactca
     7501 catctactaa cttttgtaga tgaaaaacaa atcaaagaat ttgaaaaaga gttcattcgt
     7561 gcaaacgata atagtgatac ttttggtgat ttaggaagtg gtgaaaaggc gttagaaaaa
     7621 tactatcaag cattatgcta tttagttcct ccttttaggg atatattggc taggtatagc
     7681 tttttgtcac tagatgaaaa aaaatcagtc tataatatta ttgttggttt aagtaacaac
     7741 ttatcaaaag aaaataaata actttaaaca tcatgtataa atacctaaca aaaccacgcg
     7801 ccttgcctgc tgatggaaag aaaggtacaa atacatgaat atcatagaga aaattaaaaa
     7861 gaatggccaa agagtttatt atgctagtgt ttatctaggc gttgaccaac taacgggcaa
     7921 aaaagcccgt acaactgtta cagcaaccac taaaaagggc gttaaagtaa aagcgcgtga
     7981 tgcgatcaat acttttgctg ctaatggcta tacagttaaa gataagccga caattacaac
     8041 atacaatgag cttgtaaaag tttggtggga tagttacaag aatacagtta agccaaatac
     8101 tcgccaatcc atggatggat tggttagagt gcatttattg cctgtatttg gcgattacaa
     8161 gctatctaaa cttactacgc ctattcttca acagcaagta aacaaatggg ctgacaaggc
     8221 aaataaaggc gaaaaagggg catttgctaa ctactccttg ctccataaca tgaataagcg
     8281 tattttgaaa tatggcgtag ctatccaggt aatacaatac aacccagcta atgatgtaat
     8341 cgttccacgc aaacagcaga aagaaaaggc tgctgtcaaa tacttagaca acaaagaatt
     8401 aaaacagttt cttgattatt tagacactct gaatcaatca aattatgaga acttatttga
     8461 tgttgtcctg tataagactt tattggccac tggttgccgt attagtgagg ctctagctct
     8521 tgaatggtct gatattgacc tagaaagcgg tgttatcagc atcaataaga cactaaaccg
     8581 ctatcaggaa ataaattcac ctaaatcaag cgctggttat cgtgatatac caatagacaa
     8641 agccacatta cttttactga aacaatacaa aaaccgtcaa caaattcagt cttggaaatt
     8701 aggccgatct gaaacagttg tattctctgt atttacggag aaatatgctt atgcttgtaa
     8761 cttacgcaaa cgcctaaata agcattttga tgctgctgga gtaactaacg tatcatttca
     8821 tggtttccgc catacacata ctactatgat gctctatgct caggctagcc cgaaagatgt
     8881 tcagtataga ttaggccact ctaatttaat gatcactgaa aatacttact ggcatactaa
     8941 ccaagagaat gcaaaaaaag ccgtctcaaa ttatgaaaca gctatcagca atttataaaa
     9001 aataagggtg acccatttcc gggctaccct cttactatac caaaaattag taggggtagt
     9061 aaaaagggta ttaaattata aaaagcacta agggaaagcg ccccaaagtg cttatttcaa
     9121 aggctttata gcctataatc acataaggag attatttttt aaggttgtag aatgatttca
     9181 atccacgata ttcagctact tcgccaagtt ggtcttcgat acgaagcaat tggttgtatt
     9241 tagcgatgcg gtctgtacgt gaaagtgaac cagtcttgat ttgtcctgcg ttagttgcaa
     9301 ctgcaatatc agcgattgtt gaatcttcag tttcacctga acggtgtgat acaacagcag
     9361 tgtaaccagc ttctttagcc atttcgatag cttcaaaagt ttcagtaaga gtaccgattt
     9421 ggttaacttt gataaggatt gagttagcag caccttcttg gataccacgt gcaaggtagt
     9481 cagtgtttgt tacgaagaag tcgtcaccaa caagttgtac tttcttacca agacgttcag
     9541 taagagcttt ccaaccatcc cagtcgtttt catccatacc atcttcaata gtgatgattg
     9601 ggtatttgtt aaccaattct tcaaggtagt cgatttgttc tgcagatgta cgaacagcag
     9661 caccttcacc ttcaaattta gtgtagtcgt aaactttacg ttctttatcg tagaattctg
     9721 atgaagcaca gtcaaatccg ataaatacgt ctttacctgg tacatatcca gcagcttcaa
     9781 tcgcagcaag gatagtttca acaccatctt cagttccttc gaaacgagga gcgaatccac
     9841 cttcgtcacc tacggcagtt tccaaaccac gtgatttaag gattttctta agagcgtgga
     9901 agatttcagc accgtaacga agggcttctt taaatgttgg cgcaccaact ggcaagatca
     9961 tgaactcttg gaaagcgatt ggagcgtcag agtgagaacc accgttgatg atgttcatca
    10021 ttggagttgg aagaacttta gtgttgaatc caccaaggta gctgtaaagt gggatttcaa
    10081 ggtagtcagc agcagcacga gctacagcga tagacacacc gaggattgca ttcgcaccca
    10141 atttaccttt gttaggagta ccgtcaagtg cgatcatagc acggtcaata gcttgttgat
    10201 cacgtacatc gtagccaatg atagcttcag caatgatgtt gtttacgttg tcaacagctt
    10261 tttgtgtacc aagaccaccg taacgagatt tgtcaccgtc gcgaagttca actgcttcgt
    10321 gttcaccagt agaagctcct gatggaacca taccacgtcc gaaagcacct gattcagtgt
    10381 aaacttctac ttcaagtgtt gggttaccgc gtgagtctag gacttcgcga gcgtaaacat
    10441 cagtaataat tgacattttt tactctcctt atgagttaaa ttttttacac ctctataata
    10501 ccttaaaacc cctccttttt caagaaaaaa cgttatcttt gtgcaacttt tccttaactt
    10561 tataaagtaa tcgctttctt ttgtctgttt tattctaact tttatgatat actgttttca
    10621 tgacagattt atcaaaacaa ttacttgaaa aagctcatgg tgggttaaaa ataaatccgg
    10681 atgagcaaag acgctatctt ggtacttttg aggaaagagt tcttggatat gtagatattg
    10741 acacagcaaa tagccctcag ttagaaaaag gctttttatt tattttagaa aaccttcagg
    10801 aaaaagcaga gccactattt gtgaagattt caccaactat cgaatttgat aagcaagttt
    10861 tctacttaaa agaagcaaaa gaaactgata gtcaagccac catagtatct gaagagcata
    10921 ttacttctcc t
//
LOCUS       DAVBCG010000062        10735 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBCG010000062 DAVBCG010000000
VERSION     DAVBCG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.062"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            65..247
                     /locus_tag="VQ495_001303"
     CDS             65..247
                     /locus_tag="VQ495_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2531256.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            547..1386
                     /locus_tag="VQ495_001304"
     CDS             547..1386
                     /locus_tag="VQ495_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2531257.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            1404..3242
                     /locus_tag="VQ495_001305"
     CDS             1404..3242
                     /locus_tag="VQ495_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2531258.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3255..4670
                     /locus_tag="VQ495_001306"
     CDS             3255..4670
                     /locus_tag="VQ495_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2531259.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            5262..6308
                     /gene="pheS"
                     /locus_tag="VQ495_001307"
     CDS             5262..6308
                     /gene="pheS"
                     /locus_tag="VQ495_001307"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2531260.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            6308..6817
                     /locus_tag="VQ495_001308"
     CDS             6308..6817
                     /locus_tag="VQ495_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531261.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            6894..9299
                     /locus_tag="VQ495_001309"
     CDS             6894..9299
                     /locus_tag="VQ495_001309"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2531262.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYVLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(9472..10473)
                     /locus_tag="VQ495_001310"
     CDS             complement(9472..10473)
                     /locus_tag="VQ495_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2531263.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            10629..>10735
                     /locus_tag="VQ495_001311"
     CDS             10629..>10735
                     /locus_tag="VQ495_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2531264.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASL"
BASE COUNT         3441 a         1725 c         2280 g         3289 t
ORIGIN      
        1 tttcataata aaacacccca aaagttagat tttttctgtc taacttttgg ggtgtagttc
       61 acagttgaag agccagaaaa ttaaagtgtc ttttactcaa tcaaaaatag taacgcctat
      121 tcttttggtg aataagacta ttgtttggta tggtgcaatg ccattattag ggaaggtaga
      181 tgagatgacc atattacgtt tggaatcagc tagtatagtt tctgaactag tggcaggttt
      241 acgatagaga aaatttttaa aaatttctat gtatgatttt catttcttta gtgagactgt
      301 tgccattatc acattcgaat cacacaaaat aaaaaaattt ctacaagtac ttgacaaata
      361 gattgaaata tcataaaatg aaaatggtta cagagttatt aattatttaa gcttcatgtc
      421 accattaaaa attgaaataa aaggatgtta tcactaatac aagtgagcag gaacctattt
      481 aatcacatcg gaagaagttt cttgatgttt ttaagtaggt tccttttatt ttaaaaggga
      541 aattttatga tcataaaacg aatactaaac cacaatgccg taattgcgca aagtaaaaaa
      601 gatatcgata ttcttctttt tggaaggggc atagcttttg gaagaaaaac tggagataaa
      661 gtaaatccaa ttgatattga gaaaagtttt tttctcaaaa atagagataa tatgacccgt
      721 tttacagaga tgtttattaa cgttcctttg gagttggtgt acatcaccga aaaaataatt
      781 aacctaggta aaataacatt gggtaataat tttgatgaaa ttatctatat taatttaacg
      841 gatcatattt cttcgagcat agaacgttat aaagaaggga ttattatttc gaatccccta
      901 cgctgggaaa tatcgaaata ttataaagaa gaatttgaac ttgggaaaag ggctttacaa
      961 ataataaaaa aagagttagg tattgaactt ccaattgacg aagctgcatt catagcgcta
     1021 cattttgtta atgctaattt agaaaataat tttcaagagt cgtataaaat cactgaaata
     1081 attatgggaa ttgagaaaat cattcaagat ttctattgta ctgagtttaa ccaagattct
     1141 attgattatt atagattcat aactcatata aaattatttg ctcatcgctt ggttgaaaat
     1201 acaacttatt gtgacgatga tgatgaagac ttgttagcat taatgaaaaa taaatatcct
     1261 agagaatatg aatgtggtga acaggtggca atgtttatac aaactgagta caattatttg
     1321 ctgacttcta gtgaattagt ttatttgatg gctcatattc gtcgcttaac aaaaaattta
     1381 gattaataga ataggagaat accatgagtt ataaagatac ggtacaaaaa atcctcgatg
     1441 taattggagg tgaaaaaaat gtcaatagag ttacccattg tgtaacacgt ttaagattag
     1501 aattaaaaga tgaaaattta gtcaatgatg atgatgtgaa gaagatacca gatgtaatag
     1561 gtattatgaa aaagaatgga caatatcaaa ttatacttgg taatgatgta gctaattatt
     1621 ataaagaatt cgttaaactt ggcaattttg aatccgattc agttgttcaa gggcacaaag
     1681 ggaatatttt agaaagaatc attgagtata tcgctggttc catgactcca atcattccag
     1741 caatgttagg gggaggtatg ttgaaagtct tggtaatcat tttaccaatg cttggtatat
     1801 tgcaatcaga ttctcagact attgcttttt tgacattttt tggggatgct ccatattatt
     1861 tcttaccgct gttattagct tattctgcat cacaaaaatt aaaagtaaca tctacattag
     1921 ctatgtctgt agcaggtgta cttctccatc caaattttgt tcaaatggtg caatcaggga
     1981 atcctcttag tttatttggt gcacctgtga caccagctag ttatggttca tcagtcgttc
     2041 caattcttat tatggtttgg ttgatgaaat atattgaaaa aataattgct aaattaacac
     2101 cagctattac taagagtttt ttgcaaccta cgctagtatt attagtatca agctgtattg
     2161 ccttagttgt agtcggacct attggagtaa ttgttggtga aggattatca aatctagttg
     2221 ggcaaatgta tggtgtagct ggatggctta cattagctat tcttggtgct attatgccat
     2281 ttattgttat gactggaatg cattgggctt ttgcacctat ttttttggcg gcatctattg
     2341 ctactccaga cgtattaatt cttccagcaa tgttagggtc aaacttagct caaggggctg
     2401 cttcgatggc tgttgcatta aagagtaaaa ataataatac aaaacaaatt gcttttgcag
     2461 caggtttctc agccttactt gcagggatta ccgaacctgc attatatggt gtgactttaa
     2521 aatataaaaa accgctttat gcagctatga ttggtggtgg attagcggga ttatttgcag
     2581 gtcttactag tgttaaagca tatctatttg ctgtcccatc tttgatagcg ttgcctcaat
     2641 ttatttattc tgatgtgcca tcaaatattg taaatgcttt aattgtggcg gtcatttcgg
     2701 ttgttattac ctttgtatta gcttatatat ttggaatcga tgaagaagag agttctagca
     2761 atttagaagt taaagctgga gtttcaaata aaaaaatgat attttctcct atatcaggag
     2821 aaatcattcc gttaagcgat gtccaggata aaacattttc agataaatta attggagacg
     2881 gagtagcgat tatcccaagt gaaggtaagg tttatgcacc atttgatggg aaaattacaa
     2941 atatttttcc gactaagcac gcaattggat tgaagagtga tgagggtgtt gagttactaa
     3001 ttcatattgg attagatact gttgagctaa aaggtcaagg ttttattagt catgtagaag
     3061 aaggagacag agttttcaaa aatcagttga tttttgaaat gaacttgaat ttaatcaaga
     3121 ctaaaggcta cgaaacagtt acaccagtaa ttgtaacgaa taccaatgat tttctagatg
     3181 tattagtatt acctaataat cagacaatcg agcattctaa ggaattactg gtaatattat
     3241 aatggaggaa agttatgacc atttttccag atgattttct ttggggtgga gcggttgcag
     3301 ctaatcaagt agagggagca tataatgaag atggtaaggg cttatcagtt caggatgtgt
     3361 tacccaaagg tggattagga gaagcaacag aaaatcctac agaagataac ttaaaattga
     3421 taggtattga tttttatcat aaatataagg aagatatatc cttgttttct gaaatgggct
     3481 ttaatgtttt tcgtacttct attgcatgga gtagaatttt tccaaaagga gatgaggaag
     3541 aacctaacga agctggtttg aaatattatg atgaattatt tgatgaacta catgctcacg
     3601 ggatagaacc acttgtaact ctttcacact atgagactcc attatattta gcaagaaaat
     3661 atcatggatg gattgatagg agaatgattc atttttatga aaaatttgct cgaacagttt
     3721 tagaaaggta taaagataaa gttaaatatt ggcttacatt taacgaagta aactctgttt
     3781 tggaattacc gtttactagt ggaggaatag atatacctaa ggagaatctt tcgaaacaag
     3841 aattatatca agctatacat catgaattag tcgcctcgag tttggttaca aaaattgctc
     3901 gtgagattaa ttcagagttt aaggtggggt gtatggtatt agctatgcca gcttatccaa
     3961 tgactccaaa tccaaaagat gtatgggcga ctcatgagta tgagaatcta aattatctat
     4021 tttcagatgt gcatgttaga ggttattatc cgaattatgc aaaaagatat tttaaggaaa
     4081 atgacattaa catagagttt gcagctgaag atgcagagtt attaaaaaat tatactgtag
     4141 attttttatc ctttagctat tacatgagtg tgactcaatc tgctattcca acacagtata
     4201 attcaggaga agggaatatt attggtggtt tagtaaatcc ttatttagag tcttcagagt
     4261 ggggatggca aattgatcca attggactac gtataatttt aaatagatat tatgaccgtt
     4321 atcaaatccc attatttata gtagaaaatg gattaggtgc taaagatcaa ctaataaaag
     4381 atgaacttaa taacttaacc gtccaagatg attatagaat tcagtatatg aaagaacatt
     4441 tattgcaagt tgctgaagct ctacaggatg gtgttgaaat tatgggctat acgtcttggg
     4501 gatgtattga ctgtgtttct atgtccactg cacaactttc taaaaggtac ggtctcattt
     4561 atgttgatcg aaatgatgat ggaaatggca cattcaatcg atataagaaa atgtccttta
     4621 cttggtataa aggagtgatt gaatcaaatg gagaatcctt attcaaatag tttgtttatt
     4681 gatgattaga tattatcacg gttaaggcaa catctcatta aatataaaat aaagaggttt
     4741 ggaagacatc aaattctaaa cctctttatt ttataggggg attaattaat ttaattttgt
     4801 tacagagagc cgattataaa tagttaactt aggatagatt ggttatatat agtagactga
     4861 atctaaaata gtacgaaaca attgctaaaa catttataga aattaatttt actttcccaa
     4921 tcgatttgtt ttcatcttat ttcaatccgc tataaagtta gataagaata agctaacaag
     4981 cagaagtaag ataggtcttg caagaatggt aaaaatctga taaactaagt agtaattgaa
     5041 taggaatctg aaagactagt agcccaaggg aagtatatca gggaggagag ccgtgactgc
     5101 aagctctcta tatgaaagct gggtgaattc acttgcgcat ggatttagaa atgaaggtct
     5161 gacttgtcag atgaaaacgg atggtaccgc gtgtcaacgc tccgagtgga gtttttggca
     5221 tgtggttttc tttttatcta cgagagactg atggaggaaa tatgtcaact attgaagaac
     5281 aattaaaagc gcttcgcgaa gaaacgctgg ctagcttgaa gcagattact gctggaaatg
     5341 aaaaagagat gcaagatttg cgtgtctctg tccttggtaa aaagggttcg ctcactgaaa
     5401 tcctcaaagg gatgaaagat gtttctgctg agatgcgtcc aatcatcggg aaacacgtca
     5461 atgaagctcg tgatgtcttg actgctgctt ttgaagaaac agctaagctc ttggaagaaa
     5521 agaaagtcgc ggctcaactg gctagcgaga gtatcgatgt gacgcttcca ggtcgtccag
     5581 ttgcgactgg tcaccgtcac gttttgacac aaaccagtga agaaatcgaa gatatcttca
     5641 tcggtatggg ttatcaagtc gtggatggtt ttgaagtgga gcaagactac tataactttg
     5701 aacgtatgaa ccttccaaaa gaccacccag ctcgtgatat gcaggatact ttctatatca
     5761 ctgaagaaat cttgctccgt acccacacgt ctccagttca ggcgcgtgct atggatgccc
     5821 atgatttttc taaaggtcct ttgaagatga tctcgccagg gcgtgtcttc cgtcgcgata
     5881 cggacgatgc gacccacagt caccaattcc accaaatcga aggcttggta gttgggaaaa
     5941 atatctctat ggctgatctt caaggaacgc ttcagttgat tgtccaaaaa atgtttggtg
     6001 aagagcgtca aattcgtttg cgtccatctt acttcccatt cacagagcca tctgttgagg
     6061 tggatgtttc ttgcttcaag tgtggtggag aaggctgtaa tgtatgtaag aaaacaggtt
     6121 ggatcgaaat tatgggggcc ggtatggttc acccacgtgt ccttgaaatg agtggtatcg
     6181 atgcgactgt atactctggc tttgcctttg gtcttggaca agagcgtgta gctatgctcc
     6241 gttatggaat caacgatatc cgtggattct accaaggaga tgtccgcttc tcagaacagt
     6301 ttaaataatg attagaaaag tagaaatggc agatgttgag gtgttggcta aaattgccaa
     6361 acaagccttt cgtgaaacct ttgcgtatga taatacggaa gagcagttac aggaatactt
     6421 tgaagaggct tatagtctga aaactttgtc aactgagttg ggaaatcctg actctgaaac
     6481 ctatttcatt atgcatgagg aggagatagc tggttttctc aaagtcaact ggggaagtgc
     6541 tcaaactgag agagaattag aggacgcttt tgaaattcaa cgcctctatg tgctacaaaa
     6601 attccaagga tttggactag gtaagcaact gtttgaattc gcacttgaac ttgctacaaa
     6661 aaatagtttt tcttgggctt ggctaggtgt ttgggagcat aatacaaaag ctcaagcgtt
     6721 ttataaccga tatggttttg aaaaatttag ccaacatcat tttatggttg gtcaaaaagt
     6781 agatacggat tggttactga gaaagaaatt aaggtaagaa gagaattctt ctattgaaat
     6841 gataggaaag gaaaagaacc agtgtggcaa tggtcattct ggagaactaa attatgcttg
     6901 tatcttataa atggttaaaa gaattggtgg gcattgatgt gccatcacag gagttggctg
     6961 aaaaaatgtc aaccacaggg atcgaggtcg aaggtgtcga atcaccagct gccggtcttt
     7021 caaaaattgt cgtcggtgag atcttgtctt gcgaagatgt gccagaaact caccttcatg
     7081 tttgtcaggt taacgttggc gaagaagaag agcgtcagat cgtttgtggt gccccaaatg
     7141 tgcgtgcagg catcaaggtt atggtggctc ttccgggagc tcgtatcgct gacaattaca
     7201 aaatcaaaaa aggaaaaatc cgtggcttag agtcacttgg aatgatctgt tcacttggtg
     7261 aattgggtat ttctgactca gttgtaccta agaaattcgc agatggtatt caaattttgc
     7321 cagaaaatgc cgttccaggt gaggaagtct tttcatatct agacttggat gatgaaatca
     7381 tcgaactttc tatcacacca aaccgtgcag atgccctttc tatgcgtgga gtggctcacg
     7441 aagtggcagc catatatgac aaggcagtca actttaaaga atttactttg acagaaacaa
     7501 atgaagctgc gacagatgcc ctttctgtgg gcattgagac agacaaggcg ccttactatg
     7561 cagctcgtat cttggacaat gtgactatcg caccaagtcc acaatggttg caaaaccttc
     7621 tcatgaacga aggcatccgt cccatcaata acgttgtaga cgtgacaaac tacatcctgc
     7681 tctactttgg tcaacctatg catgcttttg acttggacac atttgaaggg actgacatcc
     7741 gtgtgcgtga agcgcgtgct ggtgaaaaat tggtgacctt ggacggtgaa gaacgtgact
     7801 tggacgtgaa tgacctagtc atcactgttg cagacaagcc agtagccctt gcaggtgtca
     7861 tgggtggtca agcaacagaa atctctgaaa aatctagtca tgttgtcctt gaagctgctg
     7921 ttttcaatgg caaatctatc cgtaagacaa gtggtcgcct gaaccttcgt tctgagtcat
     7981 cttctcgctt tgaaaaagga attaatgtgg caacagttaa tgaagccctt gatgcggcag
     8041 ctagcctgat tgcggaactt gcaggtgcga cggtgcgtaa gggtatcgtt tcagcgggtg
     8101 agcttgatac ttcagatgta gaagtttctt caactcttgc tgatgttaac cgtgtcctcg
     8161 gaactgagct gtcttatgct gatgtcgaag acgtcttccg tcgtcttggc tttggtcttt
     8221 ctggaaatgc agacagcttt acagtcagcg taccacgtcg tcgttgggat atcacaatcg
     8281 aagctgatct ctttgaagaa atcgctcgta tctatggata tgaccgcttg ccaaccagcc
     8341 ttccaaaaga cgatggtaca gctggtgaat tgactgtgac acaaaaactc cgccgtcaag
     8401 ttcgtaccat tgctgaagga gcaggtttga cagaaatcat cacctatgtt ctaacaactc
     8461 ctgaaaaagc agttgagttt acggctcaac caagtaacct tacggaactc atgtggccaa
     8521 tgactgtgga tcgttcagtc ctccgtcaaa atatgatttc aggtatcctt gataccgttg
     8581 cctacaacgt ggctcgtaag aataaaaact tggcccttta cgagattgga aaagtctttg
     8641 aacaaacagg taatccaaaa gaagaacttc caaatgaaat caacagtttt gcctttgcct
     8701 tgacaggctt ggttgctgaa aaagatttcc aaacagcagc agttccagtt gatttcttct
     8761 atgctaaagg aatcctagaa gcgctcttta ctcgcttggg actagaagtg acctatacag
     8821 caacagctga gattgccagc cttcacccag gacgtacagc agtcatttca ctcggtgacc
     8881 aagttcttgg ttttcttggc caagtgcatc cagtcactgc taaggcttac gatattccag
     8941 aaacgtatgt ggctgagctc aacctttcag tcatcgaagc tgcccttcaa cctgctgctc
     9001 catttgtgga aatcacgaaa ttcccagcag tcagccgtga cgttgccctt ctcctcaagg
     9061 cagcagtaac tcaccaagaa gttatagatg ctatccaagc tgccggcgtg aaacgtttga
     9121 cagatatcaa actctttgac gttttctcag gtgagaaatt gggacttggt atgaagtcaa
     9181 tggcttatag cttgaccttc caaaatccag aagacagctt aacggacgaa gaagtcgcac
     9241 gctacatgga aaaaatccaa gcatcgcttg aaaaaaaagt gaacgcagaa gtgcgttaaa
     9301 cgatagtagg atacaaattc cccctctagt ttatgaacac tagaggggtg attttatata
     9361 atactcaatg aaaatcaaaa agcaaactag gaagctagcc gtaagctgta cttgagtact
     9421 gtaaggcgag gctgacgtgg tttgaagaga ttgtcgaaga gtataaaata attaaaatgt
     9481 gatttctgta aaaatacatc tatgatcaga gttttgaaaa cttacaatgt ctaaatcttt
     9541 aacatagtag tggtttttag gcaataaaat atgatctatt gttgcattaa aaagttttgg
     9601 actttggcta ttccaagttc ctctttcaaa aggtggtagt acatttaatg cgtccctatg
     9661 agagcttatt tttgcaagtg ctccatgacg catagttgca ttaaaatcat ctgcaataat
     9721 agcctttgga tattttgaag ccaattgatt atgaatgatg tttaagtctt gcttccagat
     9781 ttccattaaa cctggcagag gaggagcagt atgcaaggca atgatatctg gtatattttg
     9841 ttttctcgaa tgtaatacaa ttgtcccgaa ccgtgttgta tgaaaagttt tagcttctgt
     9901 atagaaacca taacttttct tgacaatcac agtcacagga gctattccac tattggtagg
     9961 tggagaagtg aaaatatcat agttggccat agaaagtcca acttgatgaa acaatagttt
    10021 gattctctgg ttttcttgct cacctctgat attggtagct agttcaggga atatagccat
    10081 atcggcgtca aaatggctaa aaattcgctc aatatgttgt gcttctattt gattagcgac
    10141 gttccaagtt actaatttta aggttttagt cttatttgat gcagaagaaa gggttttatc
    10201 tgttccgaat aatagtaata tgagcgatag taagagcaaa atgccagagt aaaaccgtat
    10261 ttgtttattt ttcttgtaaa aagataggga aaaaaatacg aaacttaaaa tagcagccat
    10321 agttgcagta gctaagggga aggcataaat ctgtgccaag ataggtctgg tagatagcca
    10381 aatattaaga ttagaagatg gaaaaaaaga ataagttatg atagggataa caaagagaat
    10441 gaccgaaagg gcagagagaa atgttttaat cattgtgtga gtacctcaat ttgcaatggt
    10501 tgctcggagc taatgtgact ttgtgacttt acttgattga tattcttttt cttgtcttcc
    10561 tcatagatta agtacaaccc aagtaaaaaa agtccaattt aagattgaaa tgattaaaag
    10621 gcacagaaat gcaagacaat tatactacaa aaggcaaaca tttgacaatc gatagccgtc
    10681 gcttaatcga aagatggaaa aaagaaggga aatcaaatag agaaattgcc tctct
//
LOCUS       DAVBCG010000063        10514 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBCG010000063 DAVBCG010000000
VERSION     DAVBCG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.063"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(69..1022)
                     /locus_tag="VQ495_001312"
     CDS             complement(69..1022)
                     /locus_tag="VQ495_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2531265.1"
                     /translation="MSISQRTTKLILATCLACLFAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(1090..2299)
                     /gene="sstT"
                     /locus_tag="VQ495_001313"
                     /pseudo
     CDS             complement(1090..2299)
                     /gene="sstT"
                     /locus_tag="VQ495_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            2438..2998
                     /locus_tag="VQ495_001314"
     CDS             2438..2998
                     /locus_tag="VQ495_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2531266.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            3055..3963
                     /locus_tag="VQ495_001315"
     CDS             3055..3963
                     /locus_tag="VQ495_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2531267.1"
                     /translation="MVLEKQLGHGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            3915..4505
                     /locus_tag="VQ495_001316"
     CDS             3915..4505
                     /locus_tag="VQ495_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2531268.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            4508..5614
                     /gene="yqeH"
                     /locus_tag="VQ495_001317"
     CDS             4508..5614
                     /gene="yqeH"
                     /locus_tag="VQ495_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2531269.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVATRKAII"
     gene            5828..6139
                     /gene="yhbY"
                     /locus_tag="VQ495_001318"
     CDS             5828..6139
                     /gene="yhbY"
                     /locus_tag="VQ495_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2531270.1"
                     /translation="MSLASKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            6191..6820
                     /locus_tag="VQ495_001319"
     CDS             6191..6820
                     /locus_tag="VQ495_001319"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2531271.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            6820..7413
                     /gene="yqeK"
                     /locus_tag="VQ495_001320"
     CDS             6820..7413
                     /gene="yqeK"
                     /locus_tag="VQ495_001320"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2531272.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            7414..7914
                     /locus_tag="VQ495_001321"
     CDS             7414..7914
                     /locus_tag="VQ495_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2531273.1"
                     /translation="MKTAFIIIDVQNILVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            7927..8280
                     /gene="rsfS"
                     /locus_tag="VQ495_001322"
     CDS             7927..8280
                     /gene="rsfS"
                     /locus_tag="VQ495_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2531274.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            8600..9340
                     /locus_tag="VQ495_001323"
     CDS             8600..9340
                     /locus_tag="VQ495_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2531275.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            9350..10447
                     /locus_tag="VQ495_001324"
     CDS             9350..10447
                     /locus_tag="VQ495_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2531276.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKVRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
BASE COUNT         3163 a         2070 c         2442 g         2839 t
ORIGIN      
        1 tgtggataga actgacgaag tcagtaacat atatacagca aggcgacgct gacgtggttt
       61 gaagagtatt actgtctata tttttggtaa aaatcaactt ttacttggat gaaggttttg
      121 gcttcacgta ggagttgaag aagggtggcg cgggtttcaa attcttctct tgtcttgggc
      181 agactgcggt tccggaagac ttccagataa cgttcaattt catctagcaa atcagaagca
      241 ggattggtct ggctcagttg acctgcaatt tttgaaaaga gttgcgctaa gatcaggctt
      301 tcactggcgg caaggtgaca agtgttaatc tgttgggcca tgtttctcag gatacgactt
      361 tgtcgttgtc tcatctcaaa gtagtggata tggtagtcgg tctggtgaaa gaggtggtca
      421 gagtgatcca aatagaccag tctgagggct tctttcaaaa gcgtgtctaa ttctgctacc
      481 agctgtgctc ggttgcgtcc gtctcctctg gataaatagt atttgaagcg ctggaggata
      541 tcttttaact tttcttccac cagcgtgtgg tagtgctgga tttcctcttc tcgtgaaggc
      601 atatagagat taacaagcaa ggcaaatcct gtaccgatag caaagagaag gaattcgttg
      661 actaggaggt ctggagaggt tgactcttga accaagagat ggctaaccaa aaccgtgctt
      721 ggtgtgatgc caatttccca gcccatcttg taggctaaag gaacgtagaa ggccagatag
      781 aggccgagac tccagatatg aaatccgctc aagtgaaaag ctagaacacc gatagccaga
      841 gctagaagca tagaaaaaag acgattgcga gccagtttta aagtacttct acgcgtatca
      901 gataggctca agagagcgat aattccagcc gaaactgctg acgaaagatt gagaaaataa
      961 gcaaacaggc aggcaagaca ggtagctaag atgagcttgg tcgtacgttg gctaatagac
     1021 ataagaattt cctaataagt tagaataaaa gcgtaaaaga caagacatga gcaggcttgc
     1081 cttgatgagt tattttttac gggttgctgc gtattcggca acggcggtaa agaggacatc
     1141 tgtagaagag ttaagggctg tttcacatga gtcttggatg acaccaatca caaaaccaac
     1201 cccaacaatt tgtatggcaa tatcgttaga aataccgaaa aggctacaag caactgggat
     1261 aagaaggagg gaacctccgg caatacctga agcaccacag gctgagatag ctgctaccac
     1321 actgaggaca aaggctgtgg caaagtcaac aggaattcca agagtgttaa ctgtaacaag
     1381 ggtcaaaagg ttaatggtaa tcgctactcc agccatattg atagtagaac cgagtgggat
     1441 agaaacagaa taggtatctg ggttgagtcc aaggtcatgg cagagtttca tattgacagg
     1501 aatgttagcc gcagaactac gagtgaaaaa ggctgtcaca ccgctgacac ggaggcagtt
     1561 ccaaactaga gggtaaggat tgcgtctcat aaagaagaag gcaatcaaag ggttgaccac
     1621 aggggcaaca aaaagcatag tcgttactaa tagaaccaat aaaataccgt agttggcaag
     1681 ccttccgact cccttgtcag aaatggtttt aaaaacaaga ccaaggattc caaatggagt
     1741 cagattgatg atccattcga caattttaga agtcacgtca gcgatagttt ttagcaattc
     1801 tttactattt ttactggctt ctctcatagc gattccaaaa atgactgccc aagataagat
     1861 tccaatatag ttagcagtaa gcagggcgtt gactgggctg tcaaccagtt tgagcaagag
     1921 gttgctgagg acctgcccaa tcccatctgg tggtgcaatt tcagtattgg cactatttag
     1981 ggtaatttca atagggacga tgaaacttgc tagtacagct acaagagcag cggcgaaagt
     2041 ccctatcata ggatatacaa gaaaacaaca gttttcatat tgctatcttg tcccttttga
     2101 tgttgggaaa gggcattggc aacgagagca aagactagga taggagcaac agcttttaga
     2161 cctccaacga ataaatcccc gagtagccca atccctgaga gattaggaag ggtcagtcct
     2221 aggattcccc acaaagcata ccaatcaaga tacgcttgac aaggcttgcc ttattccaag
     2281 catgaatgat tcttttcata ataatctcct ttttgtgtag tgattatgat tatagtataa
     2341 atgatagaca aaatcaagaa ttttctgtct attttttgaa tatttatgga gaatgagact
     2401 gatgaaaata tggtataatg aaataaagga gttttatatg caaaaattta ttcaggctta
     2461 tattgaaaag ctagatgtga caaccattat cgagaatatt ctaaccaagg tcatttctct
     2521 tttactgctt ttgattgtat tttatattgc taaaaaaatg cttcatacca tggtgcagag
     2581 aattgtcaaa ccttctctaa aaatgtctcg tcatgatgtc ggacgccaaa aaaccatctc
     2641 acgtttacta gaaaatgtgt ttaattatac gctatatttc tttttactct actgcatttt
     2701 gtcgatttta ggtttgccag tttctagttt gctggctgga gctggtattg ctggggtagc
     2761 gattggtatg ggagcccaag gctttctgtc tgatgtcatc aatggctttt tcatcctctt
     2821 tgaacgtcaa ctggatgtgg gagatgaggt cgttctgaca aatggaccga ttactgtatc
     2881 gggtaaggtt gtcagtgtgg gaattcgtac gacacagctt cgtagcgagg agcaagccct
     2941 tcactttgtc cctaaccgaa atatcacagt tgttagcaat ttctcacgca cagactagac
     3001 ctgttatttt aagtaatttg tggtacaata gagggagttt aataaggaga aaagatggtt
     3061 ttagaaaagc agttgggcca tggttgtacc tggatagacc tagacctagg aaagttgaat
     3121 aaactagaag acctttctga aatttacggt ttggacaagg aaaccattga atacgcactg
     3181 gatagaaacg agcgcgccca catggactac caccgtgaaa gtgggacggt tacctttatc
     3241 tataatgtcc tagacgtaaa aaaggacaag gcctactatg agacttttcc catgaccttt
     3301 atcgtcgagc atcgtcgcct gattaccatt agtaatacca agaacgccta tgtcattgaa
     3361 cagatgactc gttatctgga gagccatgac acgctttcga tttataagtt tctctttgcc
     3421 agtctggaaa tcatcagcaa tgcctactat cctgtcattg agcagatgga caagagtagg
     3481 gatgaggtca atgacctctt gcgccagcga actaccaaga aaaacctctt tgccctgtct
     3541 gatttggaga ctggtatggt ttatctgacg gcagctgcca aacaaaatcg gattttgtta
     3601 gagcatattc aaggtcatgc cttgtatcgt agttttgatg agattgagag agaacagttt
     3661 gatgatgcca tgattgaggc tcatcagctg gtatccatga cagacctaat ctctcagatt
     3721 ttacagcagc tttcagcctc ttacaacaat attctaaaca ataatctgaa tgacaatttg
     3781 acaaccttga ctatcatttc agtcttgcta gctgttttgg cagtcgtgac aggctttttc
     3841 ggaatgaatg ttcccttacc tttaacagat gagccccatg cttggctcta tatcagtttg
     3901 gctagtgcag gtttgtggat tgttttatcc ttgttactaa ggaaaattgc gaaaaaaagt
     3961 taagaaaagg agccagaatg gcgattgaaa attatatacc agattttgct gtggaagcag
     4021 tctatgatct gacagtccca agcctgcagg cgcagggaat caaggctgtt ttggtcgatt
     4081 tggataatac cctcattgct tggaacaacc ctgatggaac gccagagatg aagcaatggc
     4141 tacatgacct tcgggacgcg ggtattggca ttatcgtagt gtcaaataac accaaaaaac
     4201 gcgttcaacg agcagttgag aaatttggga ttgattacgt ttactgggcc ttgaagccct
     4261 tcacatttgg tattgaccgt gccatgaagg aattccacta tgacaaaaag gaagtggtca
     4321 tggttggcga ccagctcatg acagatatac gagcagccca ccgtgcaggg attcggtcaa
     4381 ttttagtcaa acccttggtc caacatgact caatcaaaac gcagattaac cgaactcgtg
     4441 agcgtcgtgt tatgagaaaa atcactgaaa agtacggacc gattacatat aaaaaaggaa
     4501 tttaactatg gaagaaattc tctgtattgg ttgtggagca accattcaga cgacagataa
     4561 agctggtctt ggttttaccc cccagtcggc acttgaaaaa ggtttggaga ctggcgaagt
     4621 ctattgccaa cgctgtttcc gtctccgcca ctacaatgaa atcacagatg tccagttgac
     4681 gaacgatgat ttcctcaagc tcttgcacga ggtgggagac agtgatgcct tagtggtcaa
     4741 tgtcattgat atctttgatt ttaatggatc tgtcatccca ggtttaccac gtttcgtctc
     4801 gggcaatgat gtcctcttgg taggaaataa aaaagatatc cttcctaagt cagttaagtc
     4861 tggtaagatt agccagtggc tcatgaaacg tgcccatgaa gaaggtcttc gtccagtcga
     4921 tgtggtccta acttcagcac aaaataaaca tgccattaag gaagtcattg ataagattga
     4981 acactaccgt aagggccgcg atgtctatgt ggtcggtgtg accaacgttg gaaaatcaac
     5041 tctaatcaat gctattatcc aagaaatcac gggtgatcag aatgtcatca ctacttcacg
     5101 cttcccaggg acaaccttgg acaaaataga gattccgctt gacgacggat cttatattta
     5161 cgatacgccg ggaattatcc accgccacca gatggctcac tacttgacgg ccaaaaacct
     5221 caagtatgtc agtcctaaaa aggaaatcaa gcctaagacc tatcagctta atcctgagca
     5281 aaccctattt ttaggtggtt tgggacgctt tgactttata gcaggagaaa agcaaggatt
     5341 tactgctttc tttgataatg aactcaaact ccatcgtagc aagcttgaag gagctagtgc
     5401 tttctacgat aagcacctgg gaactcttct gacaccacca aatagcaagg aaaaagaaga
     5461 tttcccaagg ctagtccagc atgtctttac cattaaagat aagacagacc tagtcatttc
     5521 aggcctaggc tggattcgtg taacaggcac agcaaaagtc gccgtctggg caccagaagg
     5581 cgtcgccgtc gccacacgaa aagcaattat ttaaaacaaa atcgctgagc gaacaaagtg
     5641 agctcatata gcgatagtta ttgccgtggt gtgattgcca atctgtagat tggcaagggg
     5701 cacaattgag accaaggctc aattgtgagg tcaggaaatc catttttcaa agatgaaatc
     5761 tttggaaaat tagttccgac cgtccctcac cacctaaaat aactatcaaa aaacaaggaa
     5821 gaaaattatg tcattagcat caaaacaacg tgccttcctc aacagccagg cacacaccct
     5881 caaacctatc atccaaatcg ggaaaaatgg actcaacgac caaatcaaaa ccagcgtccg
     5941 tcaagctctt gatgcgcgtg aattaatcaa ggttactctc ttacaaaaca cagatgaaaa
     6001 catccacgaa gtagctgaaa ttttggaaga agaaatcggt gtggatacag tccaaaaaat
     6061 aggacgcatc ttgattttgt ttaagcaatc tagcaagaaa gaaaatcgca agatttctaa
     6121 gaaagtcaaa gaaatctaag accgaaactc caaataactg tttttataga gaaataaagg
     6181 ggactagcct atgacaatcg aactattgac tccctttacc aaggtagagt tggagccaga
     6241 aatcaaggag aaaaaacgca aacaagttgg gattttaggg gggaatttta accctgttca
     6301 caatgcccat ctcattgttg cggatcaagt acggcaacag ttgggactgg atcaagttct
     6361 tctcatgcct gaataccaac ctcctcacgt tgataaaaag gaaaccatcc ctgaacacca
     6421 tcgtctcaag atgcttgagt tggcaattga gggaattgac ggcctagtca ttgaaaccat
     6481 tgagttggag cgcaagggta tttcctacac ctacgacacc atgaagattt tgacagagaa
     6541 gaatccagat acggattatt actttatcat cggtgccgac atggttgact atctgcctaa
     6601 gtggtaccga attgatgaac tggttgacat ggttcagttt gtgggggttc agcgtccacg
     6661 ctacaaggta gggacttcct atccagttat ctgggtggac gtaccgctca tggatatctc
     6721 gtccagcatg gtgcgggact tccttgccca aggtcggaaa cccaactttc tcctacctca
     6781 gccagtgcta gactacatcg agaaggaggg gctctactga tgtcctatca agactatatc
     6841 aactgctccc gtgaggcctt gttggaaaaa atggcagagt ttctacctga gaaacgtcta
     6901 acccattgtc taggtgtgga gcgtgccgct atggaattag ctcagcgctt tggtgtagat
     6961 gttgagaaag caagtctagc aggtcttctt catgactatg ctaaaaagtt gtcaaatcag
     7021 gaatttctag ccttgattga ccgttaccag ctagaccctg acctcaaaaa ctggggcaat
     7081 aatgtctggc atggtatggt cggaatttac aaaattcagg aagatttgga tttgcatgat
     7141 tcagaaatcc tgcgagccat tgaagtccat acagtcggtg ctggtcaaat gacagaccta
     7201 gataaggtca tctacgtcgc agactatatc gagcacaatc gagcctttcc tggagtagat
     7261 gtggcgcgtg agattgcaaa tctatcgctc aataaggcgg tggcctacga aacagctcgt
     7321 accgtggagt atctagctca tcagggattc cccatctatc cccaaaccct tgaaacctat
     7381 aacgcctttg tgcactattt gaaagagaac taaatgaaga cggcttttat aatcattgat
     7441 gttcagaata ttttagtaga aactgggttc cagacaaaaa gtctattggg aaaaatttct
     7501 tatttacaaa accaggctag aagcaagaat attgaaatta tctatgttca acatattgag
     7561 aactctgaag ctcaaacatc agaagattgg cagttatctg cgcttttaaa tcgaaaacct
     7621 gctgaaaagg tctttcagaa gaagtataac agtattttca aagaaactgg cttaaaagaa
     7681 tacttggata agcaggggat tgaaaaatta gttttatgtg gtatgcagac agaatattgt
     7741 gtggatacct ctgtcaaggt tgcctttgaa tatggctatc agcttattgt tccagaaggt
     7801 gctgtcacaa cctttgatgg ggatgacatt ccagcagaaa cgattaatga attttatgag
     7861 gacatttggg aggagcgctt tgcagatgtt ctagattaca aacatatttt ctaaaaagag
     7921 gattaaatga acgaaaaaga attactagaa ctagtcgtga aagcggctga tgagaaacgt
     7981 gcggaggata tcctcgcact tgatgtacaa gatttgacta gtgtgacgga ctactttgtc
     8041 atcactagct caatgaatag ccgtcagttg gacgctatcg cagctaatat ccgtgaaaaa
     8101 gtagctcaag caggctttaa aggtagccat gtcgaaggcg atgcagctgg aggctgggtc
     8161 ttactggacc ttggtgctgt tgtcgtgcat atcttttcag aagaaatgcg tgcccattat
     8221 aatctagaga agctatggca tgaggcgaat tcagtagata tttcagaagc tcttgcttag
     8281 aagatgaact cagttgtagt caactgggtt tctttgctta ttttagaaaa attgatagaa
     8341 aggtaaaggg cctcgtgttt gtttcatttc gaaactgaac acgagctaaa agctgagata
     8401 aaaagtaact ttctttgtat ctgacgatag ggaagaaagt ttcctatgta ctctttgctt
     8461 ttaaggctct ttgtatctta gaaaggattt gaacccgtcc tgtaattctc ggaaaataga
     8521 tagccttcct tggagctcgg ctccatcgta aggctcctaa ttttctatcg aattacgggc
     8581 ggacttggta tcatgtttta tggcgactta tgaaaccttt gcggcggtct atgatgcggt
     8641 catggacgat agtttatacg acaaatggac gaatttttct ctgcgtcatt tgcctaagac
     8701 caaggagaga aagaaactct tggaattggc ttgtgggaca ggaattcaat cagtgcgctt
     8761 ttctcaggct ggttttgatg tgactggact tgacttgagt gcggatatgt tgaagattgc
     8821 ggagaagaga gcaacttcag ccaagcaaaa gattgctttt atagaaggta atatgctgaa
     8881 tttgtccaag gcagggaaat acgattttgt cacgtgttat tcggactcta tctgctatat
     8941 gcaggatgag gtggaagtag gggacgtctt taaggacgtg tacaatgcgc tgaatgaaga
     9001 gggagttttc atctttgacg tgcactcgac ctaccagaca gatgaagtat tccctggcta
     9061 ttcctaccat gaaaatgcgg aagattttgc catgctttgg gatacatatg aggacgaagt
     9121 tcctcactcc atcgtgcatg agctgacttt ctttatcaag gaggctgacg gttcctttag
     9181 tcgccacgat gaagtacatg aggagaggac ctatgagatc ttgacctatg atatcttgct
     9241 ggaacaggct ggatttaagt ccttcaaact ctatgcagac tttgaggata aggagccaac
     9301 agaaactagc acccgttggt tttttgtggc gcagaagtag gagattagca tgaccataac
     9361 aggtattatc gcggagttca atccttttca taatggccat aaatacctgc tggatcaggc
     9421 agagggactg aaaattgttg ccatgtcagg gaatttcatg cagcgtggag agcctgctat
     9481 tgtggataag tggacaaggg cccagatggc gctggaaaat ggcgcagact tggtagtgga
     9541 attgcccttt ttagtcagtg ttcaggcagc tgatttcttt ggtcaaggag ctgtggatat
     9601 cttggatcgc ttgggcattg atagtctagt ttttgggaca gaggaagttc tggattacca
     9661 gaaaatcgct gacttataca cagagaaagg tgctgagatg gagaaatttg tgaaaaatct
     9721 gcctgattcc ctttcctatc cccagaaaac ccaagctatg tggaaggaat ttgctggtct
     9781 tgatttttca ggtaataccc ccaatcatgt tcttgctctg gcctatgcca aggcggttgc
     9841 aggacgaaat atcaagctgc atccgattca gcgtcagggg gcaggttacc attctgtgaa
     9901 taaagatgtg gactttgcct cggcgacagc cctccgtcaa catcagaagg accaagattt
     9961 cttagaacgt tttatgcctt ctgttgccct atttgagcag gccagtaagg tgatctggga
    10021 agactatttt cccttgctcc gctatcaaat cttgtcaaat cccgacctaa ccaccatcta
    10081 tcaggtcaat caagaaatgg ctgtgcgcat taaagaagct attaaaacag cccagtctgt
    10141 ggaagaattg gtcgagttag tgactaccaa acgttacacc aaggcgcgtg tcagacgcct
    10201 attgacctat attttggtac aggctagaga aaatgtcttg ccagaagcca ttcatgtcct
    10261 tggatttact gaaaaagtca ggcaacatct caagtctctg aaaggacagg tcaatctagt
    10321 cagccgaatt ggcaaagaac cttgggatgc tatgacccaa aaagcagacc agatttacca
    10381 actagggaag ccaagtatag cagagcaaaa ttttggaaga gtgccgatta gaatagaaac
    10441 aaactaagtc taccgaaagg taggcttttt gagatttttt acgaataata ctcttcgaaa
    10501 atcaaattca aatc
//
LOCUS       DAVBCG010000064        10461 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBCG010000064 DAVBCG010000000
VERSION     DAVBCG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.064"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..266
                     /locus_tag="VQ495_001325"
     CDS             <1..266
                     /locus_tag="VQ495_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2531277.1"
                     /translation="SIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGW
                     GDHYQLVAQKSVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            720..1898
                     /locus_tag="VQ495_001326"
     CDS             720..1898
                     /locus_tag="VQ495_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2531278.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            2213..2344
                     /locus_tag="VQ495_001327"
     CDS             2213..2344
                     /locus_tag="VQ495_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2531279.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2365..3915
                     /gene="dltA"
                     /locus_tag="VQ495_001328"
     CDS             2365..3915
                     /gene="dltA"
                     /locus_tag="VQ495_001328"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2531280.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEALVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQSLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3912..5156
                     /gene="dltB"
                     /locus_tag="VQ495_001329"
     CDS             3912..5156
                     /gene="dltB"
                     /locus_tag="VQ495_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2531281.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQTIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            5170..5409
                     /gene="dltC"
                     /locus_tag="VQ495_001330"
     CDS             5170..5409
                     /gene="dltC"
                     /locus_tag="VQ495_001330"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2531282.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDICVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5402..6670
                     /gene="dltD"
                     /locus_tag="VQ495_001331"
     CDS             5402..6670
                     /gene="dltD"
                     /locus_tag="VQ495_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2531283.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6825..7265
                     /gene="adcR"
                     /locus_tag="VQ495_001332"
     CDS             6825..7265
                     /gene="adcR"
                     /locus_tag="VQ495_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2531284.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            7265..7969
                     /locus_tag="VQ495_001333"
     CDS             7265..7969
                     /locus_tag="VQ495_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2531285.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7962..8768
                     /locus_tag="VQ495_001334"
     CDS             7962..8768
                     /locus_tag="VQ495_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2531286.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8778..10283
                     /gene="adcA"
                     /locus_tag="VQ495_001335"
     CDS             8778..10283
                     /gene="adcA"
                     /locus_tag="VQ495_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2531287.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDVEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT         2998 a         1956 c         2254 g         3253 t
ORIGIN      
        1 ccagcataga tgagcgcttg cgtactcgcc tacgagtgat tatctggaag caatggaaga
       61 agaaatcgag acgattatgg ggattgctta agttaggagt tcctaaatgg atagcagata
      121 aggtatctgg ctggggcgac cattatcaat tagtagctca gaagtcggta cttaaacgtg
      181 ctatatcaaa accagtcctg gaaaaacgtg gactggtttc gtgtttggat tattaccttg
      241 aacgacatgc gttaaaagtt agttgaaccg ccgtatgccg aacggcacgt acggtggtgt
      301 gagaggggct agagattatc ctctactcga ttaactcccc tgaaatttgt tttaattatg
      361 caaatttcac gtatttttga tgctgagacg acgatcctgg gaacttttca gatatttttt
      421 tgactatcta aaatctatca ttagaaaagc ttagagcgcc aaaggatttg agcgtttttc
      481 tgatttttaa gactttttcc cagtctcttt ttcgattgaa gatgtaatta ttctactaac
      541 taacttctta gtactagcca acaacgataa tcataattcc tcctaaaatt aggaataata
      601 aaggcaatag tttttgtttt ttcatgtaaa aaacctcact tttgttttct gctattttat
      661 gctaaaatat taaaaatcaa atttaattcc aaagtttgta actaaagggg gagcgctaca
      721 tgtctaattc atttgtcaag ttgttagtct ctcaattatt tgcaaattta gcagatattt
      781 tctttagagt aacaatcatt gctaacatat acattatttc aaaatcagta attgccacat
      841 cactagttcc tatcttaata ggaatatcct cttttgttgc gagtctttta gttccgttgg
      901 ttactaaaag gttagcgcta aatagggttt tatctttatc tcaatttgga aagactatat
      961 tattggcgat actggtagga atgtttaccg taatgcaatc cgtagcgcct ttggtgacct
     1021 atctatttgt tgttgcaatt tccatactag atggttttgc agcacccgtt tcctatgcta
     1081 ttgtgccacg ctatgcgacc gatttgggta aggctaattc agccttatca atgactggtg
     1141 aagctgttca attgataggt tggggattag gtggactctt gtttgcaaca attggtctgt
     1201 tacctaccac gtttatcatt ttagtcttgt atatcatttc tagctttctg atgttatttc
     1261 ttcctaacgc tgaagtggag gtgttagagt cagaaactaa tcttgaaatt ttgctcaaag
     1321 gttggaagtt agttgctaga aatcctagat taagactttt tgtatcagca aatttattgg
     1381 aaattttttc aaatacgatt tgggtttctt ccattatact tgtttttgta acggagttat
     1441 taaataaaac ggaaagttac tggggatatt ctaatacagc atactctatt ggtattataa
     1501 ttagtggctt aattgctttt aggctatctg aaaagttcct tgctgctaaa tgggaaagta
     1561 ttctttttcc tcttgtagct atggcaatag tgacactgac tattctatat tttccaaacg
     1621 cacagatgtt tttattattt tcagctttag ttggtatgtt atcgcaacta aaagaagttc
     1681 ctgaaagtgt atttcttcag gaaacagtag aggaaaatca tttagttaat gtctattccg
     1741 ttcttgaagt gatttctaca ttagcatttt cagtatttgt tttgctaatg agctatatta
     1801 ctgagagttt tggtattagc atcagttttt ggctatcagc catttgtctg atgatagaag
     1861 ctattttaat ttatatcaga cgagattatt ttaaatgaga gagtggttaa aaagtcttta
     1921 aaaatcagaa aagcgcatag tatcaggtgt tgaaaaaact tgatattgtg tgttttatta
     1981 tggggaagat ttacttcatt gtctcctgaa atggagtttt gaagcaggat cactctattt
     2041 gaaaatatac atagaaaaac tgtataatcc ttaaaataag ctgaaatcta ttggaaacat
     2101 acggtaaaca attgaaaaga tactatataa agtggtacaa tggtagaaaa ataagctagt
     2161 aagaatcact cttattttag cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca
     2221 gagaaaagaa ttgtacctct ttcttggtcg gacagccttg tattttctta tctttctagg
     2281 gctgctttac ttctttagct atcttggtca gggtcaagga agctttatct ataatgaatt
     2341 ttaacttgaa ggagaatccc tattgtgtca aataaaccaa tagcagatat gattgaaacc
     2401 attgagcatt ttgctcagac acagcctagc tatcctgtct ataatgtttt ggggcaggaa
     2461 cacacttatg gcgatttaaa ggctgattcg gatagtttgg ctgcagtcat tgaccaacta
     2521 ggcttgcctg agaagtctcc tgtggttgtt tttggtggcc aagaatatga aatgttggca
     2581 acctttgtag cgctgactaa gtcaggtcat gcctacattc caattgatag ccattcggcc
     2641 ttggagcgag tttcagctat tttagaagta gcagagccaa gcttgattat tgccatttca
     2701 gcctttccct tggagcaggt ttctacacca atgataaatc tagctcaggt tcaagaagcc
     2761 cttgttcaag ggaataacta tgaaatcacg catccagtca agggagatga taattactac
     2821 attatcttta cttctggtac gactggtaag cctaagggag tgcagatttc acatgataat
     2881 ctcctcagct ttacaaactg gatgattatg gataaggaat ttgcgacacc gagtcgtccg
     2941 caaatgctgg cacagccacc ttattctttt gacttgtctg tcatgtattg ggcaccgacc
     3001 ttggcacttg gtggtacgct tttcactctt ccttcagtca tcactcagga ctttaagcaa
     3061 ctctttgcgg ctatcttttc attgccaatc gctatctgga catcaacacc atcctttgca
     3121 gatatggcca tgttgtctga atacttcaac agtgagaaaa tgcctggagt cacgcatttc
     3181 tactttgatg gtgaagaatt gacggtcaaa acagctcaaa aactgcgcga gcgtttccca
     3241 aatgcccgta tcatcaatgc ttacggccca acagaagcga cagtagctct ctcagcagtt
     3301 gccgtgacag acgagatgtt agcgactctc aaacgcctac caatcggcta taccaaggct
     3361 gattctccaa cctttatcat tgacgaggaa ggaaataaac tgccaaatgg cgaacaggga
     3421 gaaatcattg tttctgggcc agctgtttca aaaggttata tgaacaatcc tgaaaaaaca
     3481 gcagaagcct tctttgagtt tgaaggtctg ccagcctatc acacaggcga tgtgggaacc
     3541 atgacagatg agggcttgct tctctacggc ggacgcatgg acttccagat taagtttaac
     3601 ggttaccgca ttgagttaga agatgtctct caaagcctca acaagtctcg ctttatcgaa
     3661 tctgctgtcg cagtaccgcg ctataacaag gaccacaagg tacaaaatct attggcttat
     3721 gtcatcttaa aagacggtgt tcgtgagcag tttgagcgag atatcgatat taccaaggcc
     3781 atcaaggaag acctgacaga catcatgatg tcctacatga tgccatctaa attcctttac
     3841 cgagacagtt tgccactaac tccaaatgga aagattgaca tcaaaggatt gattaacgag
     3901 gtgaataaga gatgatggag ttttttcaac agcttcctca tttagagcca tatggcaatc
     3961 ctcagtattt tgtttatgtg attgctgcaa ccttgcccat ctttataggc ctctttttca
     4021 agaaacgctt tgcctggtat gaagtgttgg taagtctctt ctttattgtc accatgttgg
     4081 tgggtggaaa gaccaatcaa ctagctgcct tgggtattta cctttgctgg gaaatattgc
     4141 tcctgctttt ctacaagcat tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag
     4201 ttagttttct gtccctactt ccgattatct ttgtcaaggt gcaaccagct atcaatggaa
     4261 cgcagtcttt gcttgggttc ttgggaattt cttacctgac ctttcgttcg gttgggatta
     4321 tcatcgagct gagagatgga gtgattaagg attttaccct ctgggaattc ctccgtttcc
     4381 ttctcttcat gccaactttc tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa
     4441 attatcagac cattcctgag cgagatgagt tgatggatat gctggatgaa tctgtccgct
     4501 atatcatgcg gggctttttg tataagttta tcctagctca tgttttagga gagaccttac
     4561 tacctcctct gaagaattta gccttgcagt caggtggctt ctttaatctc tatgccttgg
     4621 cagttatgta tacttttggt ctggagctct tctttgactt tgcaggttat tctatgtttg
     4681 ctttggccat ctcaaacttg atgggaatcc gtagccctat caactttaac aagccctttt
     4741 tatcaaggga tttaaaggag ttttggaatc gctggcatat gagtctgtcc ttctggttcc
     4801 gtgactttgt ctttatgcga atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc
     4861 gtaatgtaac ctcaagcatg gcctacattg taaatatgct gattatggga ttttggcatg
     4921 gtgtgacctg gtactatatc gcctatggac tctttcatgg actaggcttg gtcatcaatg
     4981 atgcctgggt tcgcaagaaa aaaacgctca ataaggaacg gaaaaaagca gggaaggctg
     5041 ccctacctga gaatcgctgg attcagttgc ttggtatggt tgtcactttc catgttgtca
     5101 tgttgtcatt cttaatcttt tctggattct tgaataatct atggtttaaa aaataaaagg
     5161 aaataaaata tggatatcaa atcagaagtt atcgaaatta ttgatgagtt gtttatggaa
     5221 gatgtttctg acatgatgga tgaagatctt tttgatgcag gtgtcttgga tagtatggga
     5281 acggttgagt tgattgtgga gattgagaac cgttttgaca tttgtgtccc tgtaacagag
     5341 tttggtcgtg acgactggaa tacagctaat aaaattatag ctggtattgt ggagctacaa
     5401 aatgcttaaa cgcttatgga tgatcttcgg accggtcttg atcgctggtt tgttggtttt
     5461 tctgctcatt ttcttttatc ctactgagat gcatcataat ctaggagctg aaaagcgttc
     5521 agcagtggct actactatcg atagttttaa ggagcgaagt caaaaagtca gagcactatc
     5581 tgatccaaat gtgcgttttg ttcccttctt tggctctagt gaatggcttc gttttgacgg
     5641 tgctcatcct gcggtattag ctgagaaata caatcgttcc taccgtcctt atcttttagg
     5701 acagggggga gctgcatcgc ttaaccaata ttttggaatg caacagatgt taccacagct
     5761 ggagaataaa caagttgtgt atgttatctc acctcagtgg ttcagtaaaa atggctatga
     5821 tccagcagcc ttccagcagt attttaatgg agaccagttg actagttttc tgaaacatca
     5881 atctggggat caggctagtc aatatgcagc gactcgctta ctgcaacagt tcccaaacgt
     5941 agctatgaag gacctggttc agaagttggc aagtaaagga gaattgtcga cagcagacaa
     6001 tgaaatgatt gaattattgg ctcgttttaa tgaacgccaa gcctcctttt ttggtcagtt
     6061 ttcggttaga ggctatgtta actacgataa gcatgtagct aagtatttaa aaatcttgcc
     6121 agaccagttt tcttatcagg caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac
     6181 ttccaataat gagatgggaa tggaaaatta tttctataat gagcagatca agaaggattt
     6241 gaagaaatta aaggattctc agaaaagctt tacctatctc aagtcgccag agtataatga
     6301 cttgcagttg gttttaacac agttttctaa atctaaggta aacccgattt ttatcattcc
     6361 acctgttaat aaaaaatgga tggactatgc tggtctacga gaggatatgt accaacaaac
     6421 ggtgcagaag attcgctacc agttagaaag tcaaggtttt accaatatag cagatttttc
     6481 taaggacggc ggggagcctt tctttatgaa ggacaccatt caccttggtt ggttgggttg
     6541 gttggctttt gacaaggcag ttgatccttt cctatccaat cccacaccag ctccgactta
     6601 ccatctgaat gagcgctttt tcagcaaaga ttgggcgact tatgatggag atgtcaaaga
     6661 atttcaatag ataatataat atagaagagt tcaagaatga aactagtttt cacgtttttt
     6721 cttgactctt tttttgcttg tgatataatt aactggtaaa caaaatgtca aagaatagta
     6781 tttttctctt aaaaaagaga agtctaaaag gaaaggagtc agatatgaga cagctagcaa
     6841 aggatatcaa tgcttttttg aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc
     6901 taataggtca ttgcactagc gaggtggccc tgaccaatac tcaggagcat atccttatgc
     6961 tcttgtcaga ggaatcttta acaaattcag aattggcccg tcgtctcaat gtcagtcagg
     7021 cggcagttac caaggccatt aagtctttgg tcaaggaagg gatgttggaa acatctaaag
     7081 attctaaaga tgcgcgtgtg attttttatc agttgactga cttggctcgt ccaatcgctg
     7141 aggagcacca ccatcaccat gagcatacac ttttaaccta tgaacaagtg gcgactcagt
     7201 ttactccaaa tgaacaaaaa gtgattcagc ggtttttgac tgctttagta ggagaaatca
     7261 aataatgaga tatattacgg tagaggattt gtccttctat tatgataagg agcctgttct
     7321 tgaacatatc aattatagtg ttgatagtgg ggaatttgtt accttgactg gggaaaatgg
     7381 agcggctaag acgacgctca tcaaggctag tcttggaatt ctgcaaccac gcattggaaa
     7441 ggtggctatt tcaaagacaa atacgcaagg taagaaattg agaatagcct atcttcctca
     7501 acaaattgcc agttttaatg ctggttttcc aagtacggtc tatgaatttg tcaagtcggg
     7561 tcgctatccg agaaaaggct ggttccgtcg tttgaatgct catgatgagg agcatatcaa
     7621 ggccagtctg gactcagttg gcatgtggga acatcgagac aaacgcttgg ggtctctatc
     7681 tgggggacaa aagcagcgag cggtgattgc gcgtatgttt gcttctgacc ctgatgtgtt
     7741 tatcctagac gagccgacaa cggggatgga tgcaggaagt aaaaatgaat tttacgaact
     7801 catgcaccac agcgcccatc atcatggcaa ggctgttttg atgattaccc atgaccctga
     7861 agaagttaag gattatgcgg atcgcaatat tcatctagtc cgtaaccaag actcgccatg
     7921 gcgttgtttc aacgttcatg agaatggcca ggaggtgggc catgcttagt ttattatctt
     7981 acgactttat acaacgcgcc tttttggcgg ttattgctat gagtcttttc tcaccggtat
     8041 tgggaacctt cctcatcttg cgtcgtcaga gtttgatgag tgataccctt agccacgtct
     8101 cactttcagg tgtagccttt ggtctggttt tggggatttc tccaactgtt tctactattg
     8161 ccattgtctt gattgcggcg gtctttctgg agtatctccg tacggtttac aagagcttta
     8221 tggaaatcgg gacagctatc ctcatgtcaa caggtctggc tgtttctctg attgtcatga
     8281 gcaagggtaa aagctcgagt tcaatgagtt tggaccaata tctctttggt tcgatcgtga
     8341 ctatcagtga agaacaggtc atttccctct ttgtcattgc ggcggttgtt ttgattttga
     8401 cctttctctt tcttcgtcct atgtatatct taacttttga cgaagatacg gcctttgtgg
     8461 atggcttgcc agttcgtacc atgtccattc tttttaacat ggtgacaggg gtggctattg
     8521 cccttatgat tcctgcagca ggagctcttc tggtatcgac cattatggtc ttgccagcta
     8581 gtattgccct gcgtctgggg aaaaacttta aatcggttat gctgcttgcc agtgcgattg
     8641 gctttttggg aatggtagca ggactttaca tttcctacta tgcagaaaca cctgcaagtg
     8701 caagtattac cattattttt gtaactgtct ttatactaat cagtttagta agacgtttta
     8761 tcaaatagga gacgaacgtg aaaaaaatta gcttattact agccagtcta tgtgccttgt
     8821 ttttagtggc ttgttccaat caaaaacagg cagatggtaa actcaatatc gtgacaacct
     8881 tttaccctgt ctatgaattt accaagcaag tcgcaggaga tacggctaat gtagaactcc
     8941 taatcggtgc tgggacagaa cctcatgaat acgaaccatc tgccaaggca gttgccaaaa
     9001 tccaagatgc agataccttc gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat
     9061 tgctagatac cttggataag aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc
     9121 tcttgccagg tggcgaggaa gaagagggag accatgacca tggagaagaa ggtcatcacc
     9181 atgagtttga tccccatgtt tggttatcac cagttcgtgc cattaaacta gtagagcaca
     9241 ttcgtgacag cttgtcagca gattatcctg ataaaaaaga gacctttgag aagaatgcag
     9301 ctgcctatat cgaaaaattg caagccttgg ataaggctta cgcagaaggc ttgtctcaag
     9361 caaaacaaaa gagctttgtg actcaacacg cagcctttaa ctatcttgcc ttggactatg
     9421 gactcaaaca agtcgcaatc tcaggacttt ctccagatgt agagccatca gctgctcgct
     9481 tggcagaatt gacagagtat gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa
     9541 atgcctcaca agcccttgct aacacacttt caaaagaagc aggtgtcaaa actgatgtcc
     9601 tcaatccttt agaaagtctg acagaagagg acaccaaggc tggagaaaat tacatctcta
     9661 tcatggagaa aaacctcaag gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg
     9721 agcctgaaaa ggcagaggat accaagacag tccaaaatgg ttacttcgag gatgcagctg
     9781 tcaaggaccg caccttgagt gactatgcag gtaactggca atcagtttat cctttccttg
     9841 aagacggcac gtttgaccaa gtctttgact acaaggctaa gttgactggt aagatgaccc
     9901 aggctgagta caaggcttac tatacaaaag gctatcagac agatgtgact aagattaaca
     9961 ttactgataa tactatggaa tttgttcaag gtggacaaag caagaaatat acttacaagt
    10021 atgtcggtaa gaaaattttg acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg
    10081 aagccacaga tgctgacgct ggacaattca agtatgttca gtttagtgac cacaatgttg
    10141 ccccagttaa ggcagaacat ttccatatct tttttggagg tacaagccaa gaagctctat
    10201 ttgaagaaat ggataattgg ccaacctact acccagataa cctatctggc caagaaatcg
    10261 ctcaagaaat gttggcgcat taatgagaaa tcgactagtt cataagagct agtcggtttt
    10321 ttgatactag atggctaact tcattataga cctttcagaa ctttaaacaa taaataaaac
    10381 tcttgaaata tctgggctta tatgctagaa tgtagttagt gtataaggtt cattagaaca
    10441 ccaaagcccc taactagtgc c
//
LOCUS       DAVBCG010000065        10455 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBCG010000065 DAVBCG010000000
VERSION     DAVBCG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.065"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..463)
                     /gene="nrdI"
                     /locus_tag="VQ495_001336"
     CDS             complement(<1..463)
                     /gene="nrdI"
                     /locus_tag="VQ495_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2531288.1"
                     /translation="MKIISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEK"
     gene            complement(541..1074)
                     /locus_tag="VQ495_001337"
     CDS             complement(541..1074)
                     /locus_tag="VQ495_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531289.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKASAEEEETEELA"
     gene            complement(1135..2421)
                     /locus_tag="VQ495_001338"
     CDS             complement(1135..2421)
                     /locus_tag="VQ495_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2531290.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGCD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            complement(2433..4079)
                     /locus_tag="VQ495_001339"
     CDS             complement(2433..4079)
                     /locus_tag="VQ495_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2531291.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHRASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            complement(4081..4695)
                     /locus_tag="VQ495_001340"
     CDS             complement(4081..4695)
                     /locus_tag="VQ495_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2531292.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            4830..5399
                     /locus_tag="VQ495_001341"
     CDS             4830..5399
                     /locus_tag="VQ495_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2531293.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            complement(5429..6121)
                     /locus_tag="VQ495_001342"
     CDS             complement(5429..6121)
                     /locus_tag="VQ495_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531294.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6123..7184)
                     /locus_tag="VQ495_001343"
     CDS             complement(6123..7184)
                     /locus_tag="VQ495_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2531295.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            complement(7177..8550)
                     /locus_tag="VQ495_001344"
     CDS             complement(7177..8550)
                     /locus_tag="VQ495_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2531296.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            complement(8650..9504)
                     /locus_tag="VQ495_001345"
     CDS             complement(8650..9504)
                     /locus_tag="VQ495_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2531297.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            complement(9658..10431)
                     /locus_tag="VQ495_001346"
     CDS             complement(9658..10431)
                     /locus_tag="VQ495_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2531298.1"
                     /translation="MLAACSGGAKKEGQAASKKEIIVATNGSPKPFIYEENGELTGYE
                     IEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAVNNLSYTKERAEKYLYAAPI
                     AQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQLEAYNAEHTDNPTILNYTKA
                     DFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVIELPSDQQPYVYPLLAQGQD
                     ELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK"
BASE COUNT         3029 a         2400 c         1938 g         3088 t
ORIGIN      
        1 ccttttccaa ctcatacaaa tctgcgataa tagctgcgac atgtttgata tcttccagca
       61 tgcctcgcat ttcaaagtca gccaatacag gaaagccaaa gcgttgactg tattgcttgg
      121 ctgttaggca gtattggtta ttaaagttac gatttcctga cccaaccaca ccaaaacact
      181 tactagcatt gttaccatag gcaataaaat ctcccaccgg tgtcgtcaaa atctcaacat
      241 ctccgttatc cacgccattc ccaccttcga gataggtcgg caaaaaagcg acatagggat
      301 ggtccatttc atagaaattt ttgccttcct tgactaaatc cttgatatga atcttttgaa
      361 cctcaatccc tttgtactgg gacaagagat agtctttcaa gcgcgtcaca aaactttcag
      421 tgttgccact caaactaata taaactaaag aaattatctt catatgtacc tccatgattt
      481 tatctataat caaaaaacaa ctaccaagat tgtatcttga tagttctttt ttgtcaatac
      541 ttaggcaagc tcttctgttt cctcttcttc tgccgaggct ttctgttgtc gccacatctt
      601 atagaagaca agggctagga agagaacatt gatgacgata tagaaaatgc taacagcttt
      661 tgaaccaata ctcactgtca aacgcatgct ctcatcttga accaactgca cagcgtcttg
      721 taaaccaaca tagccataaa tcgaaccaat aatagctaac aaaacatagc caacgtaagt
      781 atagtttgca tattgcaaat ttttacgaac aaggaagaca atcgctacga ccactaaaat
      841 agtagatagc acaatcaaag caacattaaa aatagaatga gatgcttctg ctactctata
      901 ggtataatta agctggtctt ctaattgctg agcattgacc cctgcaactt gtttctgagc
      961 agtacgaagg gcttctttac taggcaaggg actcacaatt ccaaacagag acggaactga
     1021 aataagggca gacaagatta gcaagaccca tagataaatt ggtttctttt tcatgataga
     1081 acctcctaat aataatattt atattatagc acattttgta aaggaataca aatcttatag
     1141 tatagtttct acctgttttc ggtaggcttt tggcgatttt ccgcacaatt ttcggaaaac
     1201 tttatagaaa tatccaaggt tactgtaacc aacagcataa caaatatctt cgatgctgtc
     1261 actagttgaa agcaaaagtt gttgggcagc cttaatacgt tgtttgttta gtaactctgc
     1321 aaaagtcgaa tcggtttcac gcttaatcaa ctgccctaga tagacaggat tgataaagag
     1381 agccttactg atatccttga gggaaagctc tttttggtaa tcacaaccaa tgacttccag
     1441 cacactgacc acattttcat tcatacggta ttgaccgaaa aagctgctca gagtttcctt
     1501 gatataagga accaattcat cgaaggattg aatagcatga atggttttga cgatgtctgt
     1561 caggtcatca gctttgagat gttcaaataa atggaaaaca tccatgacaa actgaataaa
     1621 aagctgttta gtaatcggaa cacgcggcgt attttcaaga actaccttct ccaagagatt
     1681 taactcttct acgatttgat tgagatttcc ctgaatgata accctataaa tcggttcgta
     1741 ataggcaaag agactctcag actgctgcaa atttacagaa ccgtaaaaga gggttttctc
     1801 agcattcagc ttccagcgtt caaagtgttc ttgataagga gcttcaaaag gtgtaacgac
     1861 taaaccatct aggggttggt cagaaatgaa aatctgccag tcttggccca agacatagta
     1921 gggaatggtg aaggaacctt gtttttcctt ggatagacct atccaccaat tctccttatc
     1981 ccctaaataa ctaacaaatc cagcctcgtc taattcttga ctaagagtct gacttttctt
     2041 ccctctctcg ccgagctgac ctgcaatctt ctccagcaga tttcccagct ctaccttatc
     2101 aacaggcttg accaaatagt ccaccacact aaggttcatt gctcttttta cataatcaaa
     2161 ctcctgataa cctgagagca ggatataggc agcatctggt aagatctctt tcatctcccg
     2221 aatcatatca agccctgttt tgtctggcat attgacatcg gaaatgatga catcgacagg
     2281 attttcctga acatattcta gagcttcatc ggcatgactg gctgttgcga cgacctccat
     2341 atcccactta tcaaagggaa tcaaacgctt cagaccttct gtcaccatgt actcatcatc
     2401 tactaataat actttataca ttttctccct ttctactcat cttgaattgt aatacgatac
     2461 tgaacaccgg cttgctctgc agactctatc gtaatggcat agcggtctcc aaaatagagc
     2521 acaaaacgct cgtgtacatt gacaatcccg atagactgcc tttgatcact gtagctggct
     2581 cggtgttcaa aatatttctg acttaatttt tctcggatat ttgccaactt ttcagccgac
     2641 attcctctac cattatcgac caccaaaatt tccacaaaac catcctgttt aagagccttg
     2701 atgctaatca cattatctgt ccgcctgtgg tcaacaccat gcgcgaaata gttttctacc
     2761 agcggttgca aggtaaactt gggaatcttc atattctcta actctggatc tatcttgaaa
     2821 ccataggcaa tggacttggg atagcgaacc atgcagagat agctgtattt acggcaaaat
     2881 tctaattcct gtttgaggag ggtctctctt tcgtcggaaa tattgttacg caagagacta
     2941 ctgaattcat aaatgatatc tgccaactca tcttgactct gcataactgc atacatgcgc
     3001 aagaactcca gcgtattata cataaaatga ggattgattt gcgcctgcaa ggctcgcata
     3061 ttggcatctt tttgactaag ctctaactgg taaatatcat ggatattctt ttccaatcga
     3121 tccaacatat cattggtcgt ctccgcgatt aggagtaatt cctgatcttt ttcggaaatg
     3181 tcaatccgac gcccctcttc gccttgagca atgacttgaa tggattccac taaatccact
     3241 acctgctttt ggtaattagc aaaagtctgc ctcaaagtca gataaagaat gacaataaag
     3301 agaaggctca aacccacaat caaagcagag ctagtcactg ttccttgtaa aacaaagttt
     3361 ttaggaactg ccacctgaac ctgatatcca tgagaagtta agccaactag ccaattttca
     3421 ctctccctat caactgtctc accaatatga aaaatctccg tatcaaaagg tgaggtcact
     3481 gttactgcca tcggagtatg acctctagta ttatcaatgg catggtataa aacagcagga
     3541 tccaaggaga tgtaaatcac tcctaaatct tgatctgaca ctggatctga caccggaatg
     3601 gcaaaactat ttcctgctgg tttgaaatcc tcagccctga ttttttctcc actacgtttg
     3661 tctttagtag aaacatagac ttccttgtaa tcttgaaaag caatggccac cccagttaca
     3721 aaatcatttc gaacatagag gtcatcaaca ttttcataca gagagactga tatataaggc
     3781 gaagctttcc gctctaattg ccagtaaaaa tagtctggca tgctaaggct aaagtattta
     3841 tagattccct caatacgagc ttgattttca actaaagcct gagctatctg ggttgactct
     3901 ctatggtaat attcaatttc actcactgtt ctagcagtta cacgctgggc tactctctgg
     3961 gcttcctttt cacgtgaatc ccagtcagcg taagagagta agattgcaaa actcgcaatg
     4021 ataatgatca tcactgaact ataggttctt agaagcgtat gtagccaaaa ctgcttcatt
     4081 ctattttgtc gccaattttt catggatgat ttcatagaca ggttccaaca tatcactgat
     4141 aaagaaatgc cacatcccaa ttcctacaaa aaatagcagg gcaggcatat agtaaccaat
     4201 tgctacaagt agcacagtcc caaggagaac cttggccaca gctgctagac tcataaagat
     4261 gccaatcaag gataatttga gactatttcg ataggacaag gcaaaataaa cttgtaattt
     4321 taaagatact gtataagcca aaaagatcag ggcaactact aggacattca aaagggtcgc
     4381 aatcaaatga acaatagtct gatgaggcaa ttgcaccaag agatacaaac cataggtcaa
     4441 aactagaccc acacctaaaa agctatagaa aatcaggttg cttgagacaa aattttgctt
     4501 gtaaagagac caagcctcct tcaaactgta ttcccgaaag ctataaccat gttctgcata
     4561 taagctcatc aaggtggcac tagccggcgc tagaccaagg ataatccctc ctgctaaagt
     4621 taatagccaa aagaaggctg tcgcaatcat ccctaaaaag aaacgattaa agacaaagtc
     4681 taaaaatcta cccatgatat tcccctaaaa ataactatga aaccattata tcatatttca
     4741 agcgttttca aaaacttcta gtatccattt acaatcccac caaaccatgg taaaatgaga
     4801 attgtgaaaa aattatcagg agacaattca tgaagaaatc tatcttaact acactgcttt
     4861 ttgcagttct ttacttcctc tgcatgggga ttggtgtcct tttgggcaat ctctttgacc
     4921 aaactggaaa catgttttat gcgcctgcct ttactgccct tgtcggcggt agcgtctata
     4981 tgatcctagt cgcaaaagtt ccgcgctttg gagccattac cactatcggc cttgtcattg
     5041 ccctcttttt cttgggaact aaacacggtg ctggttcctt ccttcctgga attatctgtg
     5101 gcctcctagc agatggagta gctcatttag gaaaatacaa ggacaaaaca aagaacttcc
     5161 tttctttcat tattttcgcc tttagtacaa caggaccaat cttgcttatg tggattgcgc
     5221 ccaaagccta tatggctact cttctggcaa gaggaaaatc ccaagaatat atcgaccgca
     5281 tcatggtcgc tccaaaccct ggaactgtcc ttctatttat cgcaagtatt gtcatcggag
     5341 ccctagtggg tgccttgatt ggacaagcct tgagtaaaaa atttgcacag aaaatctgat
     5401 cagttaaaaa gagccacgcg gctctttttt atttatggct caatttctta gtcaagaaat
     5461 ctcccaagaa ttggattgca aagataatca aaatgataat gatggttgcc aagatggtca
     5521 catcgtgatt gtagcggtta aatccataag cgatggctac attaccgata ccaccagctc
     5581 caaccgcacc ggccatagct gtttccccaa caagggaaat caaggtcaca gtcgtcacac
     5641 ggatcaaatc tggaagacct tctgataggt aaacacccac gatgtcccag aaagtcgctc
     5701 cgctcgcttg agccgcctca atgacaccgc catccaattc agccaagaca acctgcacct
     5761 gacgggcaaa gaaggcaaag actgcaaaag aaagtgggac aagggctgca tttggcccga
     5821 tacttgtttt aacaatcaag tgagaaagtg gtgacaagat tgccaagagg atgataaagg
     5881 gaactgcacg aaaaattgag gtaattttgt ctaaaatcca gaatacgact ttattctcca
     5941 agacaccacc tggcgctgtc aagacgagaa agagacctgc cactagcccc aagaagcctc
     6001 cgataatgaa ggaaagaact gtcatataaa gagttaagta gatagccgtt ccccagcctg
     6061 cctgaccagc ccaacccatt ttatagacat ttggtaaata ggtttgaatc aatgattcca
     6121 tcttactgtc ctcccttcaa tacttttagt tgtacacctg cttgacgaat ggcttcttgg
     6181 gcacctgcca acgctgcttt ttcacctgac aagaccacca ccaattctcc aacaggagta
     6241 ccatcgagaa tttcgatatt cccatagaga atattagcca ttacttggta atgcttgtac
     6301 aattcattca aaagtggctc gtctgttgaa gctccagcgt acttgagttg caccaagaga
     6361 ctgttttcag acaagtgttc cacgatttct tgcttctcga ttttgaccat ggcttcgtca
     6421 atacctgtag ctgttgagat aaagtcttga gtcaaaggtt gtttaggatt tgagaagatt
     6481 tcaagcacac taccctcttc aatcaaatgc ccatcctgca taactgcaac acggttggca
     6541 atgtctttga caatctgcat ttcatgcgta atcaagacaa cagtcaagcc taatttttgg
     6601 ttcaaatctt gcaacaaggc caaaatctgc ttggttgtct tcggatcaag ggcagaagtt
     6661 gactcgtctg aaatcaagat ttttggatca ttggccaagg cacgcgcaat tgccacacgc
     6721 tgtttttgcc ctccagatag ttgtgaaggg tagttttcag cacgatctgc caaaccaacc
     6781 aagtccaaca acttagctac tttagccttc ttttcttcct tgctgagtcc agagtgttta
     6841 agggcaaagg ctacattctc ctctgctgtc ttttggctca tcaggttaaa atgctggaaa
     6901 atcatcccga tatcttgacg tttacgacgc aactgctctg ccgtcaaggt gaccttacca
     6961 tcaaaaatca catcgtcgtc aatggtaatt ttccctgcag atggtttttg caagaggtta
     7021 atcacccgta caagggttga tttccctgct ccagaatatc caacgattcc gtagatatcc
     7081 ccttcttgga tgtgaatggt cacatcctta accgctgtga tggttctctt cttttggtga
     7141 aaagtcacat cgatctgatc taacttgata atatctctac tcatagcttc taatcagctc
     7201 ctctactaat tcgatatggg tgtaataatc agcgattcgc acattttcat ctccaccgtg
     7261 gtctcggcta ttggcatttc ctagaccaaa tgcaaccatt ggtacctcta gggcatcaaa
     7321 gaccgtatgc ataggtcctg tccccgctgt cgtcggcaag actgaaacgc cctgtggata
     7381 gaatttcttg gccaactcga tcacattgag aatggctggt gcgctcatat cgcttcgata
     7441 gctcatctct cccaaggtat agtataattc taccttatca aagccatttt tgtctagttg
     7501 tttccgaatt ttttccagaa catcatgcgg ttctaggccc ggaaccagac gaacctctag
     7561 cttggcactg gcttctgcag gtaaaatagt cttaacaccc tgaccttgat aaccagactg
     7621 gattccttcg atattaagcg ctggctcgaa aaagaaacgt tttagaaagg ccatccgctc
     7681 ctcctgtaag agaggcaact ccaatccata aatccgacta acttcctctg ggtttcgttg
     7741 accataagtt tctagcaagg ccatttctcg ttcattgggc tcttgtactt cttcgtacaa
     7801 gccttcaacc aagatacggc catccgcagc acgaagagac tgtaaggctt ggaggagata
     7861 ccaaggagct gattccacaa caccaccata actcgagtgg atatccacat cagcgctttt
     7921 taccttggca tcaaaggtca caatcccctt attgccacca gaaatttcca gctgttccaa
     7981 ggcatttttg gtcccttgtt cccagaccaa caaatccgcc ccacggagtt tgtctgcatg
     8041 cttttccaaa tacttatcta ggtctgttga agccgattcc tccgctccct ccatgataaa
     8101 gctgatattg acaggtaaat catcatggtg ctgcatatat tttctcaaag cactcaagcg
     8161 agctgtgata tgacccttgt cgtcatcaac cccacgccca tacatgaagc cattgcggac
     8221 cgaaagcgta aaaggatcct ctgtccagac ctgatcccca tccgctggca cagtgtcata
     8281 gtggttatag aaaatcaagg tcttggcatc tggacgcgaa ctcttgaaat gtgccatgac
     8341 aaagggcgct gtatagctct catcaatctc cacttcagct ccaacacgct tgaaaatctc
     8401 acccagataa tttgcgactt ccttgagtcc aacctgctgg gcaaagactg atttcttaga
     8461 aatcaaggta cgcaaaacct caaaataatg ttggactaca tgatcctttt caaatttttc
     8521 aatctgttct tgttcgctag gaaaaaccat attctctcta cctttctatg aacgaatagt
     8581 cttctttaca atctatactc tatacaaaag caagaggtgg aattttctct cccacctccc
     8641 tgtttcttat taccaaactg gttgatccaa accatctgat gattcttcga taactttttt
     8701 cacgtcatct gtgtggtaag ctgcgattac tttcttgata gcatcagcct taggtgatgt
     8761 ttcccaatct tttttcgcaa caatgatgtt gtaccattgt tttgagtttt catcagcttg
     8821 ttctttgaaa agtgctttct tgtagtccaa ttttgcttct gtaacgaagg tattgtttac
     8881 aacggcagcg tcaactgatg acaatgaacg agctgtttgg ctagcgtcca attcagtgat
     8941 tttcaagttc tttggatttt ctttgatgtt ggcaactgtt gcaagagcag ttccagaaac
     9001 atccaattta atcaagccag ctgattgaag caagtaaagc gcacggcttt cgtttgtagc
     9061 gtcattcggt acagcgattt ctccgtttgc tgggatgtct tctactttag tgtacttgtt
     9121 ggcacttcca ttcaaacctg agtaaaggcg gattggagag atgtaagtat ctgcaatcgc
     9181 tacaaggtct tttccgtttt ctttgttcca gttgttcaag aagttatagt gttggaaagc
     9241 gttcaaatct acttcgccat cagcagttgc tttgtttggt tgtgagtagt ctgtgaactc
     9301 tgtaaattcc aaggtaattc cgtctttttt aaccaattct tggattttgt cccaacgttt
     9361 ttcttcagaa ccgctacggt taacagttgc gattttgatc gttgttgcat tgtctgcttt
     9421 cttttctgag tttccgcaag ctgcaagagc caaacctgcg actgtagcaa gggctgctag
     9481 gccaagccat tttttgattt tcatgattct ttctccttaa aaataatacc gtactagtat
     9541 ctcacaattt gtttgatttc acaaattgtt attagatagt cagatatata gtttaaaact
     9601 atatccctaa aaactgagaa accacccatc ttactcagaa tggatgattt caagaaatta
     9661 tttaatatca gcttctgccg gtagataagt gtctccgaag aattgtttag acaatttttc
     9721 aagcgttccg tctttgtaga gttctttaat gcgtttgtct acaaatgatt tcaactcatc
     9781 ttgaccctga gcaagaagtg ggtaaacgta cggttgttgg tcgcttggaa gttcgataac
     9841 tttcaagttg tccaaacctt ggttcttgat cactgtttca acaccgattt tatcaaaaat
     9901 cttgtagtca aattgtccat cgctcaaacg taccatgatt tgttggaaat ctgccttagt
     9961 atagttaagg atagttgggt tgtccgtgtg ttcagcgttg tatgcttcta actgcttagc
    10021 tgatgtagtg gcttgaacga cttccgtcga ttttccaccg atatcatcga gagacttgat
    10081 actagagtca tctttcttca ctacaaggac attaggattt tgggcaattg gtgcggcata
    10141 gaggtatttc tccgcacgtt ctttagtgta gctaagattg ttgacagcca tattgtaacg
    10201 gtcagcatca agaccagcaa agacacctga ccattctgtc ttttcaaact tgacattata
    10261 tttgtcagaa tctttaaaga tagcgcgaac gacttcaatc tcataaccag tcaattcgcc
    10321 attttcttca tagataaatg gctttggtga tccattggtt gcaacgatga tttctttctt
    10381 gctagctgct tgtccttctt tcttagcacc acctgagcaa gccgcaagca cacctgcagc
    10441 aacaagagca agggc
//
LOCUS       DAVBCG010000066        10438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBCG010000066 DAVBCG010000000
VERSION     DAVBCG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.066"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            210..623
                     /gene="rpsL"
                     /locus_tag="VQ495_001347"
     CDS             210..623
                     /gene="rpsL"
                     /locus_tag="VQ495_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2531299.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            643..1113
                     /gene="rpsG"
                     /locus_tag="VQ495_001348"
     CDS             643..1113
                     /gene="rpsG"
                     /locus_tag="VQ495_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2531300.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1538..3619
                     /gene="fusA"
                     /locus_tag="VQ495_001349"
     CDS             1538..3619
                     /gene="fusA"
                     /locus_tag="VQ495_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2531301.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3723..8114
                     /locus_tag="VQ495_001350"
     CDS             3723..8114
                     /locus_tag="VQ495_001350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2531302.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFQTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPVEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8216..8479
                     /locus_tag="VQ495_001351"
     CDS             8216..8479
                     /locus_tag="VQ495_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2531303.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8472..8750
                     /locus_tag="VQ495_001352"
     CDS             8472..8750
                     /locus_tag="VQ495_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2531304.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8771..8923
                     /locus_tag="VQ495_001353"
     CDS             8771..8923
                     /locus_tag="VQ495_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531305.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            8945..10186
                     /locus_tag="VQ495_001354"
     CDS             8945..10186
                     /locus_tag="VQ495_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2531306.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENVGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10196..10426
                     /locus_tag="VQ495_001355"
     CDS             10196..10426
                     /locus_tag="VQ495_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2531307.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
BASE COUNT         3073 a         2024 c         2471 g         2870 t
ORIGIN      
        1 cagaaattga tctctaacat taccaataaa aatgaaaaat atcttgacaa gcaagggaaa
       61 attttgttac aataatagac ggtacttttt acttttggtc tctcaagagt gtacagggac
      121 gtgctgacaa atgttgcaaa agtacacaca gatgatatct gtcaccaagt gtatcatcac
      181 caaaaataaa aaaacacagg agaatgtaga tgcctacaat taaccaattg gttcgcaaac
      241 cgcgtaaatc aaaagtagaa aaatctaaat caccagcttt gaacgttggt tacaatagtc
      301 ataaaaaagt tcaaacaaac gtttcttcac cacaaaaacg tggtgttgca actcgtgttg
      361 gaacaatgac acctaaaaaa cctaactcag cccttcgtaa attcgctcgt gtacgtttga
      421 gcaaccttat cgaagttact gcctacatcc caggtatcgg acacaacttg caagagcaca
      481 gcgtggtgct tcttcgcggt ggacgtgtaa aagaccttcc aggggtacgt taccatatcg
      541 tccgtggtgc acttgatact gcaggtgtta acgatcgtaa acaaggccgt tctaaatacg
      601 gtactaaacg tccaaaagca taaggaaagg ggataaagag aaatgagtcg taaaaataga
      661 gctccaaaac gtgacgtatt gccagatccg ctatacaatt cacaactagt tactcgtctt
      721 atcaaccgcg ttatgcttga tggtaaacgt ggtactgctg cttcaatcgt ttacggtgct
      781 tttgagcaaa tcaaagaagc tactggcaac gatgcacttg aagtatttga aacagctatg
      841 gaaaacatca tgcctgtact tgaagtacgt gcacgtcgtg ttggtggttc taactaccaa
      901 gtcccagttg aagttcgtcc agaacgtcgt acaacacttg gacttcgttg gttggtaaca
      961 atcgctcgtc ttcgtggtga acacacaatg caagaccgtc ttgcaaaaga aatcttggat
     1021 gctgctaaca acactggtgc agctgttaag aaacgtgaag atactcaccg tatggctgaa
     1081 gctaaccgtg cattcgcaca cttccgttgg taagatagga tgcgaaagcg ttaagaaagt
     1141 cccagagaaa atagggaatc gaagcaggtt gcggttgcaa ccaatgagat tcatcttttt
     1201 ctccagactt ttagcttgag ctcaactaaa tcatgatgct aggcacggta aggatgcaag
     1261 gtgaaaatag gaaactgacg cagtattcaa cgaatacaag gagttttatc tttttcacgc
     1321 agcatcccgt tccagctcac atcggctaac taactttagc ccgggttcaa attagctaaa
     1381 tcgattagta ttagctataa ctcagcttac catctcgtaa gttgaaacca acaatagcat
     1441 gaaaacattg agaacgggta ggtcctgcct atccgttttt attaaaatcg tgttataata
     1501 gaatagaaat caaaaataaa taggagaaac aaacctcatg gcacgcgaat tttcacttga
     1561 aaaaactcgt aatatcggta tcatggctca cgtcgatgcc ggtaaaacaa caactactga
     1621 gcgtattctt tactacactg gtaaaatcca caaaatcggt gaaactcacg aaggtgcgtc
     1681 acaaatggac tggatggagc aagagcaaga acgtggtatc acgatcacat ctgctgcgac
     1741 aacagctcaa tggaacaacc accgcgtaaa catcatcgac acaccaggac acgtggactt
     1801 cacaatcgaa gtacaacgtt ctcttcgtgt attggatggt gcggttaccg ttcttgactc
     1861 acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaactg agtacggagt
     1921 tccacgtatc gtatttgcca acaaaatgga caaaatcggt gctgacttcc tttactctgt
     1981 aagcacactt cacgatcgtc ttcaagcaaa tgcacaccca atccaattgc caatcggttc
     2041 tgaagatgac ttccgtggta tcattgactt gatcaagatg aaagctgaaa tctatactaa
     2101 cgaccttggt acggatatcc ttgaagaaga catcccagct gaataccttg accaagctca
     2161 agaataccgt gaaaaattga ttgaagcagt tgctgaaact gacgaagaat tgatgatgaa
     2221 atacctcgaa ggtgaagaaa tcactaacga agaattgaaa gctggtatcc gtaaagcgac
     2281 tatcaacgtt gaattcttcc cagtattatg tggttcagcc ttcaaaaaca aaggtgttca
     2341 attgatgctt gatgcggtta tcgactacct tccaagtcca cttgacatcc cagcaatcaa
     2401 aggtattaac ccagatacag acgctgaaga aactcgtcca gcatctgacg aagagccatt
     2461 tgcagctctt gccttcaaga tcatgactga cccattcgta ggtcgtttga cattcttccg
     2521 tgtttactca ggtgttcttc aatcaggttc atacgtattg aatacttcta aaggtaaacg
     2581 tgaacgtatc ggacgtatcc ttcaaatgca cgctaacagc cgtcaagaaa tcgacactgt
     2641 ttactcaggt gatatcgctg ctgccgttgg tttgaaagat actacaactg gtgactcatt
     2701 gacagatgaa aaatctaaaa tcatccttga gtcaatcaac gttccagaac cagttatcca
     2761 attgatggtt gagccaaaat ctaaagctga ccaagacaag atgggtatcg cccttcaaaa
     2821 attggctgaa gaagatccaa cattccgcgt tgaaacaaac gttgaaactg gtgaaacagt
     2881 tatctcaggt atgggtgaac ttcaccttga cgtccttgtt gatcgtatgc gtcgtgagtt
     2941 caaagttgaa gcgaacgtag gtgcgcctca agtatcttac cgtgaaacat tccgcgcttc
     3001 tactcaagca cgtggattct tcaaacgtca gtctggtggt aaaggtcaat tcggtgatgt
     3061 gtggattgaa tttactccaa acgaagaagg taaaggattc gaattcgaaa acgcaatcgt
     3121 cggtggtgtg gttcctcgtg aatttatccc agcggttgaa aaaggtttgg tagaatctat
     3181 ggctaacggt gttcttgcag gttacccaat ggttgacgtt aaagctaagc tttatgatgg
     3241 ttcatatcac gatgtcgact catctgaaac tgccttcaag attgcggctt cactttccct
     3301 taaagaagct gctaaatcag cacaaccagc tatccttgaa ccaatgatgc ttgtaacaat
     3361 cactgttcca gaagaaaacc ttggtgatgt tatgggtcac gtaactgctc gtcgtggacg
     3421 tgtagatggt atggaagcac acggtaacag ccaaatcgtt cgtgcttacg ttccacttgc
     3481 tgaaatgttc ggttacgcaa cagttcttcg ttctgcatct caaggacgtg gtacattcat
     3541 gatggtattt gaccactacg aagatgtacc taagtcagta caagaagaaa ttattaagaa
     3601 aaataaaggt gaagactaat ccgtcctcac tctagaagga agtcacttag tggcttcctt
     3661 ttgtctttag aaaatacctc taaatatggt aaaatagtag aagaataatg tgaggaaaat
     3721 gaatgtcaaa tagttttgaa attttgatga atcaattggg gatgcctgct gaaatgagac
     3781 aggctcctgc tttagcacag gccaatattg agcgagttgt ggttcataaa attagtaagg
     3841 tatgggagtt tcatttcgta ttttctaata ttttaccgat tgaaatcttt ttagaattaa
     3901 agaaaggttt gagcgaagaa ttttctaaga caggcaataa agctgttttt gaaattaagg
     3961 ctcggtctca agaattttca aatcagctct tgcagtccta ctatagggag gctttctctg
     4021 aaggtccatg tgctagtcaa ggttttaagt ccctttatca aaatttgcaa gttcgtgctg
     4081 agggtaatca gctatttatt gaaggatctg aagcgattga taaggaacat tttaagaaga
     4141 atcatcttcc taatttagcc aaacaacttg aaaagtttgg ttttcaaact tttaactgtc
     4201 aagtcgagaa gaatgatgtc ctgacccaag agcaggaaga ggcctttcat gctgaaaatg
     4261 agcagattgt tcaagctgcc aatgaggaag cgctccgtgc tatggaacaa ctggagcaga
     4321 tggcacctcc tccagtggaa gagaaaccag tctttgattt tcaagcgaaa aaagctgcag
     4381 ctaaacccaa actggataag gcggagatta ctcctatgat cgaagtgacg acagaggaaa
     4441 atcgtctggt atttgaaggg gttgtttttg atgtggagca aaaagtgact agaacaggtc
     4501 gtgttttaat caactttaaa atgacggact atacttcaag tttttctatg caaaagtggg
     4561 ttaaaaacga ggaagaggcc cagaagtttg acctcatcaa gaagaattct tggctccgag
     4621 ttcgagggaa tgtggagatg aataacttca cacgcgattt gactatgaac gtacaggatc
     4681 tgcaggaagt tgttcactat gagcggaagg atttgatgcc agaaggtgag cgtcgggttg
     4741 agtttcatgc tcatactaac atgtcgacta tggatgcttt gccagaggtc gaagagattg
     4801 ttgcaacagc tgctaagtgg ggacacaagg cggttgctat cacagaccat gggaatgtcc
     4861 agtcctttcc acatggctat aaggcggcta agaaagcggg aatccagctg atctatggga
     4921 tggaagccaa tatcgtggag gaccgtgtcc ctatcgtcta taacgaagtg gagatggact
     4981 tatcagaagc aacctacgtg gtctttgacg tggaaacgac gggactttca gctatctata
     5041 atgacttgat tcaggttgcg gcctctaaga tgtacaaggg gaatgttatt gctgaatttg
     5101 atgaatttat caatcctggg catcccttgt cagcttttac tacagagtta actggaatta
     5161 cagatgatca tgtcaaaaat gccaaaccac tagaacaagt tttgcaagaa ttccaagaat
     5221 tttgcaagga tacggtccta gttgcccaca atgctacctt tgacgttggc tttatgaatg
     5281 ctaattatga gcggcatgac cttccaaaga ttagtcagcc agttattgat acgctggagt
     5341 ttgctagaaa cctctatcct gagtataaac gccatggttt ggggcctttg accaagcgtt
     5401 ttggtgtggc cttggaacat caccacatgg ccaactacga tgcggaagcg actggtcgtc
     5461 tgcttttcat ctttatcaaa gaggtagcag aaaaacatgg tgtgaccgat ttagctagac
     5521 tcaacattga tctaatcagt ccagattctt acaaaaaagc tcggatcaag catgcgacca
     5581 tctatgtcaa gaatcaggta ggtctaaaaa atatctttaa gctggtttcc ttgtctaata
     5641 ccaagtattt tgaaggagtg ccacggattc cgagaacggt tctagatgcc catcgagagg
     5701 gcttgatttt aggttcagcc tgttcagagg gtgaagtttt tgacgtggtc gtttctcaag
     5761 gtgtggatgc ggcggttgag gtggccaagt attatgattt tatcgaggtc atgccaccgg
     5821 ctatctatgc acccttgatt gccaaagagc aggtcaagga tatggaggaa ctccagacca
     5881 ttatcaagag tttgatagag gttggagacc gccttggcaa gcctgttctg gctacgggaa
     5941 atgttcacta tatcgaaccg gaagaagaga tttatcgtga aattatcgtc cgtagtttgg
     6001 gacagggtgc gatgattaat cgaactatcg gtcatggtga acatgcccaa ccagcaccac
     6061 ttccaaaggc tcattttcga acgactaatg agatgttgga tgaatttgcc tttttgggag
     6121 aggaactggc tcgtaaactg gttattgaaa acaccaatgc cttggcagaa atatttgaac
     6181 ccgttgaagt cgttaagggt gacttgtata cgcctttcat cgacaaggct gaagaaacag
     6241 ttgctgagtt gacctataag aaagcttttg agatttatgg aaatccgctg ccagatattg
     6301 ttgatttgcg gattgaaaaa gaattaacat ccatactggg gaatggattt gctgtgattt
     6361 atctggcatc gcagatgctg gtgcaacgtt ctaatgaacg gggttatttg gttggttctc
     6421 gtgggtctgt cggatctagt ttcgttgcga ccatgattgg gattacggag gtcaatcctc
     6481 tctctcctca ctatgtctgt ggtcagtgtc agtacagtga gtttatcaca gatggttcgt
     6541 acggttcagg atttgatatg ccccataagg actgtccaaa ctgtggtcac aaactcagta
     6601 aaaacggaca ggatattccg tttgagacct tccttggttt tgatggggat aaggttcctg
     6661 atattgactt gaacttctcg ggagaagatc agcctagcgc ccacttggat gtgcgtgata
     6721 tctttggtga agaatatgcc ttccgtgcgg gaacggttgg tacggtagct gccaagactg
     6781 cctatggatt tgtcaagggt tacgagcgag attatggcaa gttttatcgt gatgcagagg
     6841 tggaacgcct cgctcaagga gcggcgggtg tcaagcggac aacaggccaa cacccggggg
     6901 gaatcgttgt tattccgaac tacatggatg tctacgattt tacgcctgtc cagtatccag
     6961 cagatgatgt cacggctgaa tggcagacca ctcactttaa cttccacgat atcgatgaga
     7021 acgtcctcaa actcgatgta ctgggacatg atgatccgac catgattcga aaacttcagg
     7081 atttgtctgg tattgaccct aatgaaattc ctatggatga cgaaggcgtg atggcactct
     7141 tttctgggac tgatgtgcta ggggtaacac ctgaacaaat tggaacgcct acgggtatgt
     7201 tggggattcc agagtttgga acaaatttcg tacgtggaat ggtagacgaa acccatccga
     7261 caacctttgc ggaattgctt cagctgtctg gtctgtcaca cggtactgac gtttggttgg
     7321 ggaatgctca ggatctgatt aagcaaggaa tagcggacct atcgactgtt atcggttgtc
     7381 gggacgacat catggtttac ctcatgcatg cgggtctgga acctaagatg gcctttacca
     7441 ttatggaacg ggtacgtaag ggtttgtggc taaagatttc agaagaggag agaaatgact
     7501 atatcgaagc catgaaggct aataaggtgc cagagtggta tatcgaatcc tgtgggaaaa
     7561 ttaagtacat gttccctaag gcccatgcgg cagcctacgt tatgatggcc ttgcgtgtag
     7621 cttacttcaa ggttcaccat cctatttatt actactgtgc ttacttctcc attcgtgcta
     7681 aggcttttga tatcaagacc atgggtgcgg gcttggaggt catcaagcgc agaatggaag
     7741 aaatctctga aaaacggaag aacaatgaag cctctaatgt ggaaatcgat ctctatacaa
     7801 ctcttgagat tgtcaatgag atgtgggaac gaggtttcaa gtttggtaaa ttagatctct
     7861 accgtagtca ggcgacagag ttcctcatcg acggggatac ccttatccca ccatttgtag
     7921 caatggatgg tctgggagag aacgttgcca agcaactggt gcgggcgcgt gaagagggag
     7981 aattcctctc taaaacagaa ctacgcaagc gtggtggact ctcatcaacc ttggttgaaa
     8041 agatggatga gatgggtatt cttggaaata tgccagagga taaccagttg agtttgtttg
     8101 atgagttgtt ttaaaaaatt gcttaataat ctattaaaag aggctaacgt atatccaata
     8161 gatttacatt agctttcttt tttgttaaaa tagtctatgg aaatagggtg agagtatgtc
     8221 aaagatgagt ataagcatcc gtctggatag tgaggttaag gagcaggccc aacaggtgtt
     8281 tagtaatctg ggaatggata tgacaacagc tattaatatt ttccttcgtc aggcaattca
     8341 atatcaggga ttaccttttg atgttagact agacgaaaat cggaagttgc tccaagtgtt
     8401 aacagattta gaccaaaatc gtaatatgag ccagtctttt gaatcagtct cagatttgat
     8461 ggaggactta cgtgcttaag attcgttatc ataaacagtt taaaaaagat tttaagttgg
     8521 ctatgaagcg tggtttgaag gccgaattat tagaagaagt tttgaatttt ctggttcaag
     8581 aaaaagaaca tcctgccaga tatcgtgatc attcattgac ggcatccaag cattttcaag
     8641 gagttcgtga atgccatacc cagccagatt ggcttttggt ttataaagta gacaagtcgg
     8701 aattgatttt aaatttgctg aggacaggca gtcacagtga tttattttaa tctattttaa
     8761 gggggttctc atgaaactaa gaatatttgc ggaagataag ccggctaaga aggtatttga
     8821 atatcaatta gaacttgctg atcgtacaat tcttctatcg acagcactct tgtcaggtgc
     8881 tattgcttta gcaggaatct tttctgcctt gaaagaaaaa taaaaataga aaagagaaaa
     8941 cagaatggtt ttaccaaatt ttaaagaaaa tctagaaaaa tatgcgaaat tgttggttgc
     9001 gaacggaatt aacgtgcaac ctggtcacac tttggctctc tctattgatg tggagcaacg
     9061 tgaattggca catctaatcg tgaaagaagc ttatgccttg ggtgcgcatg aggtcatcgt
     9121 tcagtggaca gatgatgtca ttaaccgtga gaaattcctc catgccccga tggagcgttt
     9181 ggacaatgtg ccagaataca agattgctga gatgaactat cttttggaga acaaggctag
     9241 ccgtcttggg gttcgttcat ctgatccagg tgccttgaac ggagtggacg ctgacaagct
     9301 ttcagcttct gctaaagcta tgggacttgc catgaagcct atgcgtatcg caactcaatc
     9361 taacaaggtt agctggactg tagcagctgc agcaggactt gagtgggcta agaaagtctt
     9421 cccaaatgct gcgagcgacg aagaagcagt tgatttcctt tgggaccaaa ttttcaaaac
     9481 ttgccgtgtc tatgaagcag atcctgttaa ggcttgggag gaacatgcag ccattctcaa
     9541 gagcaaggca gatatgctta ataaggagca attttcagcc cttcactaca cagcgccagg
     9601 aacagattta acacttggtt tgccaaagaa ccacgtttgg gaatcagctg gtgctgtcaa
     9661 tgcacagggc gaaggattct tgccaaatat gccgacagaa gaggtcttca cagcgcctga
     9721 cttccgtcgt gcagatggtt atgtcacttc tacaaaaccg cttagctaca acggaaatat
     9781 cattgaaggc attaaggtga cctttaagga tggacaaatc gtagatatca ctgctgagaa
     9841 gggtgatcag gttatgaaag accttgtctt tgaaaatgtg ggtgcgcgtg ccttgggtga
     9901 atgtgccttg gtaccagatc caagtccaat ttctcagtca ggcattacct tctttaacac
     9961 ccttttcgat gaaaatgcgt caaaccactt ggctatcggt gcagcctatg cgactagcgt
    10021 tgttgatgga gcggagatga gcgaagagga gcttgaagct gcagggctta accgttcaga
    10081 tgttcacgta gactttatga ttggttctaa ccaaatggat atcgatggta ttcgtgagga
    10141 tggaacgcga gtacctcttt tccgtaatgg gaattgggca aattaaggag ataatatgtt
    10201 aggaagtatg ttcgttggtc tcctagtggg atttttagca ggtgctatga ccaatcgtgg
    10261 agagcgaatg ggatgttttg gaaaaatgtt tctcggttgg atcggagcct ttctaggtca
    10321 cttgctcttt ggaacttggg ggccagtttt atcaggaaca gctattatcc cagcgatttt
    10381 aggagccatg attgttttag ctattttttg gagacgagga agttaatttc ctaaatct
//
LOCUS       DAVBCG010000067        10319 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBCG010000067 DAVBCG010000000
VERSION     DAVBCG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.067"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            86..1261
                     /gene="mutY"
                     /locus_tag="VQ495_001356"
     CDS             86..1261
                     /gene="mutY"
                     /locus_tag="VQ495_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2531308.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1316..2020
                     /locus_tag="VQ495_001357"
     CDS             1316..2020
                     /locus_tag="VQ495_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2531309.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2013..3362
                     /gene="vicK"
                     /locus_tag="VQ495_001358"
     CDS             2013..3362
                     /gene="vicK"
                     /locus_tag="VQ495_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2531310.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3364..4173
                     /locus_tag="VQ495_001359"
     CDS             3364..4173
                     /locus_tag="VQ495_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2531311.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4264..4506
                     /locus_tag="VQ495_001360"
     CDS             4264..4506
                     /locus_tag="VQ495_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2531312.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4508..4762
                     /locus_tag="VQ495_001361"
     CDS             4508..4762
                     /locus_tag="VQ495_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2531313.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4809..5582
                     /locus_tag="VQ495_001362"
     CDS             4809..5582
                     /locus_tag="VQ495_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2531314.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(5842..6828)
                     /locus_tag="VQ495_001363"
     CDS             complement(5842..6828)
                     /locus_tag="VQ495_001363"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2531315.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7022..9490
                     /gene="gyrA"
                     /locus_tag="VQ495_001364"
     CDS             7022..9490
                     /gene="gyrA"
                     /locus_tag="VQ495_001364"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2531316.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            9490..10233
                     /gene="srtA"
                     /locus_tag="VQ495_001365"
     CDS             9490..10233
                     /gene="srtA"
                     /locus_tag="VQ495_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2531317.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3205 a         1752 c         2303 g         3059 t
ORIGIN      
        1 agatagaaaa gttatatact atctattgaa aatattataa atcagataca aagacaggtg
       61 aaaccttctg tgttatacta gagatatgtt agatttgaaa gaatacggta tcgtcatgtg
      121 gccggaggag aaagtcattt ctttccgtga gaaacttctc gcttggtatg atgaaaacaa
      181 aagagatttg ccttggagga gaagtaaaaa tccttatcac atctgggtat ctgaaatcat
      241 gcttcagcag accagggtgg atacagttat cccttactac gaaagattcc tggactggtt
      301 tccaactgtc gaaagtctgg caactgcgcc tgaggagagt ttactgaaag cttgggaggg
      361 cttgggctat tattctcgag ttcgcaatat gcaggctgca gcccagcaga ttatgactga
      421 ctttggtggc caatttccaa atacctatga aggaatttcc agcttgaaag ggattggacc
      481 ttacacagca ggagccattt ccagtattgc ttttaacttg cctgagccag ctgtagatgg
      541 taatgtcatg cgggtcttgg cgcgtctgtt tgaagtcaac cacgatattg ggattccaag
      601 taatcgaaaa atttttcagg caatgatgga gatcttgatt aatccggatc gtccgggtga
      661 ttttaatcaa gccttgatgg acttagggtc tgatattgag tctcctgtaa atcccagacc
      721 tgaagaaagc ccagtcaagg actttagtgc ggcatatcag aatggaacca tggaccgtta
      781 tccaattaaa tctcctaaga aaaagccggt tcctatttat cttaaagcat tagttgtgaa
      841 aaattctcaa ggacaatttt tacttgaaaa aaatgaaagc gaaaagctct tggcaggttt
      901 ttggcatttc ccccttatag aagttgataa cttttcgcaa gaagagcagt ttgacctctt
      961 tcatcaggtt gcggaagaaa gtgtgaactt tggccccagt ccagaagaga gtttccagca
     1021 ggactatgac ctagatgttg attggcttga tgtttgtttt gatacagtcc agcatgtttt
     1081 tagtcatcga aaatggcatg tacagattgt agcaggtcag gtgagtgact tccatgattt
     1141 ttcagatagg gaagttcgtt ggctttcacc agaagaattc aagaattacc cacttgccaa
     1201 accccaacaa aaaatctggc aggcttatgc acaagccaac ttagacagta gccaagacta
     1261 gctattgtgt cttctgacta ttttttggta taatagtaga gaaaaaggtg aacatatgaa
     1321 aaaaatacta attgtagatg atgagaaacc aatctcggat attatcaagt ttaatatgac
     1381 caaggaaggt tacgaagttg taactgcttt taatggtcgt gaagcgctag agcaatttga
     1441 agcagagcaa ccagatatta ttattctgga tttgatgctt ccagaaattg atggtttaga
     1501 agttgctaag accattcgta agacaagcag tgtgcccatt cttatgcttt cagccaaaga
     1561 tagtgaattt gataaggtta tcggtttgga acttggggca gatgactatg taacgaaacc
     1621 cttctccaat cgtgagttgc aggcgcgtgt taaagctctt ctgcgtcgtt ctcaacctat
     1681 gccagtagat ggtcaggaag cagatagtaa acctcaacct atccaaattg gggatttaga
     1741 aattgttcca gacgcctacg tggctaaaaa atatggcgaa gaactagact taacccatcg
     1801 tgaatttgag cttttgtatc atttagcatc gcatacaggt caagtcatca cgcgcgaaca
     1861 cttgcttgag actgtctggg gttatgacta ttttggtgat gtccgtacag ttgatgtgac
     1921 tgtacgacgt ctgcgtgaga agattgaaga tacgcccagc cgaccagagt atatcttgac
     1981 gcgccgtggt gtagggtatt acatgagaaa taatgcttga tttactgaaa caaaccattt
     2041 ttaccagaga ttttatcttt atcctgattt tgttaggttt catccttgtt gtgaccctct
     2101 tattactgga aaatagacgt gataatattc agttgaagca aatcaatcaa aaggttaaag
     2161 atttgattgc aggagattat tccaaggttc ttgatatgca aggtgggtct gaaatcacca
     2221 atattaccaa taatttgaat gacttgtcgg aggttattcg tctcactcag gaaaatctag
     2281 aacaagagag taagaggcta aatagtattc tgttttatat gacagatggg gttcttgcga
     2341 ccaaccgtcg gggtcagatt atcatgatta acgatacagc caagaagcaa ctggggttgg
     2401 ttaaggaaga tgttctgaat agaagcattt tggaattgct caagatagaa gaaaactatg
     2461 aattgcgtga tttgattacc caaagtccag aattgttgct agattcccaa gatatcaatg
     2521 gcgaatattt gaaccttcga gttcgctttg ccttgatacg tcgagagtct ggctttattt
     2581 caggtttggt ggctgttttg catgatacga cggagcagga gaaggaagaa cgcgaacgaa
     2641 gactctttgt ttccaatgtt agccatgagt tacggactcc tctgactagc gtaaaatcct
     2701 atcttgaagc cttggatgag ggggctttgt gtgaaactgt agcaccagac tttatcaagg
     2761 tttctcttga tgagaccaac cgtatgatgc gcatggtgac ggatctcctc catctttcac
     2821 gtattgataa tgctaccagt cacctagatg tggaactgat taacttcact gcttttatta
     2881 cctttatcct caatcgtttt gacaagatga aaggacagga aaaggagaaa aaatatgagt
     2941 tggtgagaga ttatcccatc aattctatct ggatggaaat tgatacagat aagatgacgc
     3001 aggttgtcga caatatttta aataatgcta ttaagtattc gccagatggg ggtaaaatca
     3061 ctgtcagaat gaagacaact gaagaccaga tgattttatc catttctgac cacggtttgg
     3121 ggattcctaa gcaggattta ccacgtatct ttgaccgttt ctatcgtgtg gatcgtgcta
     3181 gaagtcgtgc acaaggtggt acaggtctag gactgtctat cgctaaagaa attatcaaac
     3241 aacataaggg ctttatttgg gccaagagtg aatacggcaa gggttcaacc tttaccattg
     3301 tgctccctta tgataaggat gcagtgaaag aagaagtatg ggaggatgaa gtagaagact
     3361 agaatgagtg aaataggctt taaatacagt attttagcgt cgggttccag tggaaattct
     3421 ttttatctgg aaacctcaaa aaagaagctt ttagtagatg caggcttgtc tggcaagaaa
     3481 attaccagtc tgctagctga aattaaccgt aagccagaag acctggatgc catcttgatt
     3541 acccatgagc attcagatca tatccatgga gtaggcgttt tggctcgcaa gtatggtatg
     3601 gatctttatg ccaatgaaaa gacctggcaa gctatggaaa atagtaaata tcttggcaag
     3661 gtggattctt cgcaaaagca tatttttgaa atgggtaaaa ccaaaacctt tggagatatc
     3721 gacatcgaga gttttggtgt aagccatgat gcagtcgcac cgcagttcta tcgctttatg
     3781 aaggatgata agagttttgt cctcttgaca gatacaggtt atgtcagtga ccgtatggcg
     3841 ggcattgtcg aaaatgcgga tggttatctt atcgaggcca accatgatgt agagattttg
     3901 cgatcaggtt cttacgcttg gcgcctcaaa caacgaatcc tatctgacct tggtcacctt
     3961 tctaacgagg acggtgctga agctatgatt cggacgctag gaaatcgtac taagaagatt
     4021 taccttgggc atttatctaa ggaaaacaat atcaaggaac tggctcatat gaccatggtc
     4081 aatcagctgg ctcaagctga tctgggagtc ggagtagact ttaaggttta tgatacctca
     4141 ccagataccg caacaccatt gacagagata tagaaagaac gctgagaagg tgttcttttt
     4201 atattgactg gacacctaaa aagtaataca atggtgttac cattaaaaaa gggagcacaa
     4261 aagatgacta ctattacatt aaaagtttct gaagctgata aaacatttat gaaagcaatg
     4321 gctaagtttg aaggagtttc cctttcggaa cttattcgca ccaaaactct tgaagcccta
     4381 gaagatgaat acgatgctcg tgtggcagat ttagcctatc aagagtattt agaagacttg
     4441 gaaaaaggag ttgaacccat tacttgggaa gaaatgatgc atgatttagg cttgaaggat
     4501 gaataatttg tataaattag ttccaacaag acgttttatc aagcaattga aaaaattgga
     4561 ccgttatacg cagaagctaa ttacaaacta tttacaaacc aatgttttgg aagatccaag
     4621 acgacacgga aaggctttgg ttggtaatcg cgttggtcaa tggcgctata gaattggtaa
     4681 ttatcgagtt atcgtacaaa ttgtagatga tgaattagtt gttgctactc tagaagttgg
     4741 tcatcggaga gatatttatt aaattaaagc tgagtaataa cagtatagaa agtaaggaga
     4801 gtaaaaattt gaagaaaaaa tggttttttg cagattatta tgacacaaca attattttac
     4861 ttgctttaat ctctgttatt ctggtattac ttggatttgc tgagatgatt gatttggaca
     4921 atccgcctta tagtattatt gatttagtaa tctggggtgt ttttgtaatt gattatagtt
     4981 ggcgcttttt tacgactaaa agaaaatggc gctttattct tgaaaatatt tttgatttat
     5041 tggctattct ccctttaaat gctattttta cagtgtttcg attagggcgt atctttcgtt
     5101 tagctaaact aacaaaatta ctgaaactga ctcgtttact tagaataatt ggacttacag
     5161 gtaaattaga aagaaaaatt agtagattct tacggacaaa tggcctgatt tatatcttgt
     5221 atgttaatat ctttattgtt ctagtaggga gttcgatttt atccgttgtt gaagaaaaat
     5281 ccttctcaga tagtctttgg tgggctcttg tgacagtaac tactgttgga tatggggata
     5341 ttgttcctgc ttccattttt gggaaatggc tagcagtttt actgatgctt gtcggtattg
     5401 gaacaatagg gatgttaaca agtgctttga cgaacttttt tgtaaaggat aatccagatg
     5461 aacagataaa acttgataaa ctcaaagatg aattatcgag ccaaagaata ttactagaga
     5521 aacaatctaa aaagatagaa gaactacata aaatgataca agatttgatt gaaaaaacat
     5581 aataaaaata aagatgagac aaagtttgag ctgaggagaa atcaagtaag ttttgacaca
     5641 atagaacgca tattatcagg tataaaaact tgatactatg cgttttatag agaaataaaa
     5701 taaaatctga acaaatcaat taggaattta aagcatttta taacaacgtt ttagagtgat
     5761 ggggtgctat ttcaacttca acctactata atacagaaaa aaacaactcc ctgatgattc
     5821 aaggagttgt ctatagttaa attagttttt agaagcttct tggaattctg gatttttcca
     5881 tgcttcgtca ataatagctt gcaattcttt agcagatgct tgcatttttt gagtttctgc
     5941 atcgttcaat ggaatgttta ctggacgaac gataccatgt gcaccaacaa cagctggttg
     6001 accgataaag acattctcaa ctccgtatag accttcttgg aatactgaaa gtggaagtac
     6061 tgcgttttca tcgtcaagga ttgctttagt gatacgagca agggctactg cgataccgta
     6121 gtatgttgca ccttttttgt tgatgattgt gtaggctgca tcacgaacac cttcgaacaa
     6181 ttcaatcaat tcagcttctt gaacattttg agtgtcttta aggaattctt caaggtttac
     6241 accagcgatg ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt cacccatgat
     6301 gtaggcgtgc actgaacgag catccacatc caatttttca gcaagtgctt gacggaaacg
     6361 agctgagtca agtgaagtac ctgaaccgat aacgcgttct ttagggaaac cagagaattt
     6421 ccaagttgag taagtcaaaa cgtcaactgg gttagcagca acaaggaaga tacctttgaa
     6481 accagattca acaacttgag ttacgattga tttgttgata gcaaggtttt tacctacaag
     6541 gtcaagacga gtttcacctg gtttttgagg tgcacctgca gtaatcacaa caaggtcagc
     6601 gtctgcacag tcagagtatt gagctgcata gattttttta ggtgaagtga aggcaagggc
     6661 gtgactaagg tcaagcgcat caccaacagc tttttcatgc aattgtggaa tttcgataat
     6721 tccaagctct tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg aacctacagc
     6781 accatcaccg acaaggataa cttttttgtg ttgtttagtt gaagtcattg ttttaaacat
     6841 ctccttaatt ttattagggg attttcccta gacaacttca ttctatcact tttaaaaaac
     6901 tttgtcacga atatgcctta tagttctcga tgtaaacgtt ttagtggttt agaggctgaa
     6961 atagatggga atttatggta taatgttgtt acttactaat tgtgaaatga ggcatttatt
     7021 aatgcaggat aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg caagttttat
     7081 cgactacgcc atgagtgtta tcgtagcgcg agctcttcct gatgttcgag atggcttaaa
     7141 acctgttcac cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc cagacaaacc
     7201 ccataaaaaa tctgctcgta ttacagggga tgtcatgggt aaatatcacc cacacgggga
     7261 ttcctctatt tatgaagcca tggtccgtat ggctcaatgg tggagctacc gttacatgct
     7321 tgtagatggt catgggaatt ttggttccat ggatggagat agtgctgccg ctcaacgtta
     7381 taccgaggca cgtatgagca agattgctct ggaaatgctt cgtgatatca acaaaaatac
     7441 agttgatttc gttgataact atgatgccaa tgaacgggaa cccttggtct tgccagcgcg
     7501 ttttccaaac cttttggtta atggagcaac tggtatcgcg gttgggatgg caaccaatat
     7561 tccacctcat aatctgggtg aaaccattga tgcagtgaag ttggtcatgg ataatcctga
     7621 agtgactacc aaggacttga tggaagtctt gcctggacca gattttccaa ctggtgctct
     7681 tgtcatgggg aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt cgattgccct
     7741 acgttctcgt acagagattg aaacgactaa gactggtcgt gagcgcatcg ttgtaacaga
     7801 atttccttac atggtcaata aaaccaaggt gcatgagcat attgttcgct tggttcagga
     7861 aaaacgcatt gagggtatca cagcagtacg tgatgagtca aaccgtgaag gtgttcgatt
     7921 tgttattgaa gtcaagcgcg acgcctcagc caatgttatt ctcaataacc tcttcaaaat
     7981 gacccaaatg caaaccaatt ttggtttcaa tatgctcgct atccaaaatg gtataccgaa
     8041 aattttgtct cttcgtcaga ttttggatgc ttatatcgag caccaaaaag aagtggttgt
     8101 tcgtcgtaca cgttttgata aggaaaaagc ggaagcgcgc gctcatatct tagaaggtct
     8161 cttgattgcg ctagaccata tcgacgaagt gattcgtatc atccgtgcta gtgaaacgga
     8221 tgcggaagct caagctgagt tgatgagcaa gtttaagctt tctgaacgtc aaagtcaagc
     8281 tatccttgat atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga ttcaatctga
     8341 gtatgatgac ctcttggctc tgattgcgga tttagcagat attcttgcta agcctgaacg
     8401 tgtttctcaa attatcaaag acgaattgga tgaagttaaa cgtaaatttt ctgataagcg
     8461 ccgtacagag ttgatgattg gacaggtctt gagtctcgag gatgaggact tgattgaaga
     8521 atcggatgtc ttgattaccc tttctaacag aggctacatt aagcgtttgg atcaggacga
     8581 gttcactgct caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga aagatgatga
     8641 ctttgttcgt gagttagtgt caactagcac ccatgatcat ctgctcttct tcacaaacaa
     8701 gggacgtgtc tatcgtctta aaggttatga aattcctgag tatggtcgga ctgccaaagg
     8761 gctaccagta gtcaatctct tgaaattgga tgaagacgaa agtattcaga cggttatcaa
     8821 tgttgagtct gatcgcagtg atgatgctta tctcttcttt acaacccgtc acggtattgt
     8881 gaagagaacc agtgttaagg agtttgccaa tattcgtcaa aatggtctca aagcgctgaa
     8941 tttaaaggat gaagatgagt taatcaatgt cttgttggca gaaggagata tggatattat
     9001 cattggtacc aagtttggtt atgcagttcg ctttaatcaa tcagccgttc gtggtatgag
     9061 tcgtatcgcc actggtgtga aaggtgttaa ccttcgtgaa ggagacacag ttgttggtgc
     9121 cagcttgatt actgatcaag atgaggttct tattatcaca gaaaaaggat atggtaagcg
     9181 tacagtcgct actgaatacc caacaaaagg tcgtggtggt aagggaatgc agacagctaa
     9241 aattaccgaa aaaaatggct tgctggccgg tcttatgact gttcaagggg atgaggattt
     9301 gatgattatc actgatacag gtgtcatgat tcgaaccaat cttgccaata tttcacaaac
     9361 aggacgtgca actatgggag ttaaagtaat gcgcctggat caagatgctc agatagtgac
     9421 tttcacaacg gttgcggtgg cagaaaaaga agaagttggg acagaaaacg aaacagaagg
     9481 tgaagaataa tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa tctgctgaca
     9541 aatatcctag caggatttct gatattactg tcactggctt tgatttttaa tactcaaatt
     9601 cgaaatattt tcatagtctg gaataccaat aagtatcaag ttagccaggt atcaaaagaa
     9661 aaattagaag aaaatcagga tacagaaggc aattttgact ttgattctgt caaagctatc
     9721 tcttcggaag ctgttctaac ttctcaatgg gatgctcaaa aattaccagt tattggggga
     9781 attgcaattc ctgaattgga aatgaatttg ccgattttta aaggacttga taatgttaat
     9841 ctcttctacg gagctggtac aatgaaacgc gagcaagtaa tgggagaagg aaattatagt
     9901 ctagctagtc accatatctt tggtgttgat aatgctaata aaatgttatt ttctccttta
     9961 gataatgcta aaaatggcat gaagatttat ctaaccgata aaaataaagt ttatacttat
    10021 gaaatacgtg aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga tgatagagat
    10081 ggggtcaatg aaatcacatt agtaacctgt gaagaccttg ctgctacaga acgtattatt
    10141 gtcaaaggtg atttgaaaga aacaaaagat tattcacaaa catctgatga aattctaaca
    10201 gctttcaatc aaccatataa acaattttat taatacaaat cagtgaaatc atggatttca
    10261 ctgatttttt aagattttca taaaaatata gtaaaatgaa ataagaatag gacgaatcg
//
LOCUS       DAVBCG010000068        10193 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBCG010000068 DAVBCG010000000
VERSION     DAVBCG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.068"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            51..299
                     /locus_tag="VQ495_001366"
     CDS             51..299
                     /locus_tag="VQ495_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531318.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            430..1602
                     /locus_tag="VQ495_001367"
     CDS             430..1602
                     /locus_tag="VQ495_001367"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2531319.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1602..2876
                     /locus_tag="VQ495_001368"
     CDS             1602..2876
                     /locus_tag="VQ495_001368"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2531320.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2912..3877)
                     /locus_tag="VQ495_001369"
     CDS             complement(2912..3877)
                     /locus_tag="VQ495_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2531321.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQTIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3858..5312)
                     /locus_tag="VQ495_001370"
     CDS             complement(3858..5312)
                     /locus_tag="VQ495_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2531322.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKSQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5689..7572
                     /locus_tag="VQ495_001371"
     CDS             5689..7572
                     /locus_tag="VQ495_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2531323.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKEEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7721..8608
                     /locus_tag="VQ495_001372"
     CDS             7721..8608
                     /locus_tag="VQ495_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2531324.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGTSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8928..9545)
                     /locus_tag="VQ495_001373"
     CDS             complement(8928..9545)
                     /locus_tag="VQ495_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2531325.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(9960..10193)
                     /locus_tag="VQ495_001374"
                     /pseudo
     CDS             complement(9960..10193)
                     /locus_tag="VQ495_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         2943 a         2009 c         2279 g         2962 t
ORIGIN      
        1 tactttcctt attgtctgta aatctactga ccttgttgtt tatcccagtc atggtttcta
       61 gttcgggctc agagtttcaa agtggatggc aagagcatca attgattgct gagaaggtta
      121 gtaaaacact tgataagaca tttgataagg atgtcagaga aattccgacc agtcagtttt
      181 atcaaaaatt tgtagatgag atgggaagga tttactcagg aaatttgatc ctccaggagc
      241 tgataactgt gaatggagct tataaagcta cttatatcgg tgaactctct agcaactaat
      301 cattctttaa aatgatgacc aataagaggc ttgatttggt gaatcaagtc ttttttctct
      361 cttttactgc catttgtgat aaaatagtat agaacgattt tttacatgaa tgataaaaca
      421 gaggtaaata tgacaatcgg tattgataag attggttttg cgaccagtca atatgtcttg
      481 aaattacaag acttagcaga agcgagggga attgaccctg aaaaattaag taaaggactc
      541 ttactcaagg aattgagtat tgcgccccta actgaggaca tcgtgacctt ggcggccagt
      601 gctagtgact ctattttaac tgagcaagaa agacaagaag ttgacatggt cattgtggct
      661 accgagtcag gaattgacca gagtaaggct gcggccgtct ttgtgcatgg cttgctgggc
      721 atccagccct ttgctcgtag tttcgagatt aaagaagcct gctatggggc gacagctgcc
      781 ctccattatg ccaaattgca tgtggaaaat tctccggagt ccaaggtctt ggtcattgcc
      841 agtgatattg ccaaatatgg tattgaaact ccgggagaac caactcaagg tgctggaagt
      901 gtagctatgt tgattacaca aaatccacgc atgatggcct ttaataatga caatgtagct
      961 cagacccgtg acatcatgga tttctggcga ccaaattact cgacaactcc ttatgtaaat
     1021 ggtgtctatt ctacccaaca atacttggat agtttgaaaa cgacttggct tgaatatcaa
     1081 aaacgctacc agcttactct ggatgatttt gcggctgttt gtttccactt gccttatcct
     1141 aaattagcgc taaaaggctt gaaaaaaatc atggataaga gcctgcctca agagaaaaaa
     1201 gacctcttac aaaagcattt tgaccagtct attctctaca gtcaaaaggt ggggaatatc
     1261 tacacaggtt cacttttcct tggacttttg tctctcttgg aaaatgcaga tagcttgaaa
     1321 gctggggata aaatcgccct ttatagttac ggaagtggag ctgtggctga gttcttcagt
     1381 ggtgaattgg ttgaaggata tgaagcttat ttggataaag accgcttgaa caagctcaac
     1441 caacgaactg ccttatccgt tgcagactat gaaaaggtct tttttgagga agtaaacttg
     1501 gatgaaacaa actctgccca gtttgctggc tatgaaaatc aagattttgc cttggttgaa
     1561 attctcgacc accaacgccg ttatagcaag gttgaaaaat aatgaagata agttggaatg
     1621 gattttctaa aaaatcatac caagagcgcc tcgagctgtt aaaagctcag gcgctcctta
     1681 gtcctgagag acaagctagt ctggagaagg atgaacagat gagtgtgact gtggcagacc
     1741 agctgagtga gaatgtagtg ggaacttttt ctctgcctta ttcgctggtt ccggaggtac
     1801 ttgtcaacgg tcaggaatac accgttccct atgtgacaga agaaccctct gtggttgcgg
     1861 cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg ttttactgca caagtccatc
     1921 agcgccagat gattgggcag gtagcccttt atcaagttgc taatcctaaa ctagcgcaag
     1981 agaagattgc cagcaagaaa gcggagctct tggagtttgc caatcaagcc tatccttcta
     2041 tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga gcagataaaa ggcgaaccag
     2101 actttctcgt tgtttatatt catgtcgata cccaggaagc catgggtgcc aatatgctca
     2161 acaccatgct ggaagccttg aaaccagtct tagaagaact cagtcaggga cagagtctca
     2221 taggaatcct gtccaactac gcgaccgatt ctctggtgac tgcaagctgt cgcatggcct
     2281 ttcgctactt gagccgccaa aaggatcaag gacgagagat tgcggagaaa attgcgttgg
     2341 ctagtcagtt tgcgcaggct gatccttacc gagctgctac tcataataaa ggaattttta
     2401 atggtattga tgcgattttg attgccactg gtaatgactg gcgtgccatc gaagctgggg
     2461 cccatgcctt tgccagtcga gatggacgct atcaaggtct tagttgctgg acgctggacc
     2521 ttgaaagaga agaattggtc ggtgagatga ccctgcccat gcctgtagcg actaagggtg
     2581 gctctatcgg cctcaaccca cgtgtagctt tcagtcatga tctactagga aatccttctg
     2641 ccagagaatt agcccagatt atcgtgtcca tcggtcttgc ccaaaatttt gcagccctca
     2701 aagctttggt aagtacgggc atccagcaag gccacatgaa actacaggcc aaatccctag
     2761 ctctcctagc tggggctagt gaatctgaag ttgctcccct agtagagcgc ctcatctcag
     2821 ataaaacctt taacctagag acagcccagc gctatctcga aaatttaaga tcataaaaaa
     2881 ctcagacgaa tcggtctgag ttttcttgtg tttaaatact ttttcctggt aatagtgtaa
     2941 ctggtaagaa gtaaccagtt gtggcaactt ccttgccttc aatcttttgc aagagaagat
     3001 caatagtgag acaagcaatc tcttccaaag gttgcttgat agtagccaat tgagggtagt
     3061 aattttcgat aaagtaggtc ccatcatagc cgatgacctt gagctctttt gggacagaaa
     3121 tgcccaattc ttgagcgatt ttaatgacca gaatagctgt caaatcatcc gaagcaaaaa
     3181 tggcatctgg tttttcccgg gtcaagatat tcttgatttc catttctttt ctgacgggag
     3241 aaaagtcact ggaaacattg ataataggag cttttgggag tacggatgca aaaccagcgt
     3301 ggcgtagtcc ggttggcgaa ttggaattgt cattccctgt aatcatgatg atagtctggg
     3361 cacctgtctt gaccaaggtt tgggcagcaa gaaccccacc agcatagttg tcagaggaga
     3421 cgacagggat gtctggcgat aggtttcggt caaaggaaat aatcggcgct gtcacacgat
     3481 tgtagtcttc gattcctagg ttgtgactac cagaaatgat gccgtccacc tgattggctt
     3541 ccaacatttc gatgtattcg cgttccttct cagaatcatg ttcactgttg cagatgatgg
     3601 tcttgtaacc atttttgaag agttggtgtt ccaatttatc aatcaattct gcatagaaaa
     3661 cattggaaat attagggaaa atcaagccga ttaacttagc tgattttcct tgcagactac
     3721 gagccaggtt gttgggttta tagcccaatt ctcgcatggc ttcattgact ttttggatgg
     3781 ttttctcaga tagataccct tttttattga taacccgaga aacggtagta ggactgacgc
     3841 ctgcaagttt ggcgacatca gttagttttg cgaccataat ctaattcata gtaagttcca
     3901 gttgggtttc cagatttgat caggatacca ttttggtccg catgtgggaa gacacgacca
     3961 gaaaatactt tttctccttt attgatgaaa atttcaaaga cagagttatc gatgaagatt
     4021 gtagcagtag tagcctgatt ctcgataggg caagaacgag ttgtcccaaa ttcttgggca
     4081 tactgttctc cagcctggct acgatccact gtcacttgac cgtttacaag gtcaaagttg
     4141 attgaaagtc ccttaccttc tttatcagca agtaagacaa tctcgctctg gctattagct
     4201 tccaagttga gttcaagttc gtaagtgttc ttggtttggg aacggtttga gaaggcttct
     4261 tcagaagcac gaaggtcctt aatagcagcg actggatact ggtagagctt gtcgtctttg
     4321 atagtgagtt ccttgaccaa agagaaggtt ccttggtggt caaaacggtc agatgggtaa
     4381 gaaacatctg gcaaaccaag ccagctaact gctagagcac gcccatcagg agcgttgaag
     4441 gcttgagttg cataggcttc gaaaccgtaa tccatgtttt gaagttgaga cacatctacc
     4501 attttggcat ttttagggtc aaaggaagcc ccaatcttat acatatttgg aaagatatta
     4561 tcgtagtcta gaactttctt atccaatcct tgtggacagt agagaaggac aggatgttcc
     4621 tctacaaaga ccagattagg acattccatc atgtaggcag tacggtcgtt agcaaagtca
     4681 aggtcgccaa ctgcttgcca gtttgtgtag tcgttattga cagccttgta gagacggacg
     4741 aagccttttt tctccaagtc ctgtccaccg acgacagcat agtattgact tttaaagtta
     4801 aaaatttgtg gatcgcggaa gtggtcagta gagtctgctg gctggtcaat caagatcttg
     4861 tcaatctttg taatcttacc ctccttgtcc atcaaagcac cgatctggta tgggtgacgg
     4921 atccagtttt catcgcgaac atttcctgta taaaatagga ataagttatc gccaaattgc
     4981 atggcagaac cagagtaggc accgtggcta tctaatggag tatctggtaa aactttgatt
     5041 ccagtttctt taaagtgaac caaatcatca ctttctagct gtgcccaaga tttcaaaccg
     5101 tgggctgcac caaaaggaaa attctggtaa aagaggatcc acttgccatc aaagtaagaa
     5161 aagccatttg ggtcgttgag aagtcctgtt tttggctcaa catggtaatg agtatgccat
     5221 ggagattgtg ccatattttc ctttatatgt tgaatttctt cttgcgtcca atcttgataa
     5281 agtctgtaac gacgctcagt tgtccattcc attcattcct ccaaaaatct tatcactttt
     5341 aatagtaact gttttcgata aaaaatgcaa gccttttatg ttaaaaaaga ggaaaaactg
     5401 tcaaacgatt atcataaaat aaatacagga aatcaatcaa atttttacga aaatgtgaaa
     5461 gataatgaaa taaaagactt tttaaccaag gtttcaaact tatttttcaa ggaattcctg
     5521 atgaaacagt caaaaacaat caatctaaaa cttgcccact aagtggatag aatatttttt
     5581 ctgcaaaacg cttgacatat ttttaaaaca tgataaaata atagtcgtaa gggcgaataa
     5641 aatcgctttc aaacaagaac aaaatgttat ataaggagat ttttgcaaat gaacaatcag
     5701 gaaattgcaa aaaaagtcat cgatgccttg ggcggacgtg aaaatgtcaa tagtgttgcc
     5761 cactgtgcga ctcgtctacg tgtcatggtc aaagatgaag agaaaatcaa taaagaagtg
     5821 attgagaact tggaaaaagt tcaaggtgct ttctttaact cagggcaata ccaaattatc
     5881 tttggtacag gtacagttaa caaaatgtac gatgaagttg ttgtacttgg cttaccaaca
     5941 tcatctaagg atgacatgaa agaagaagtt gctaaacaag ggaactggtt ccaacgtgct
     6001 atccgtactt tcggtgatgt tttcgttcca atcatcccag ttatcgtagc gacaggtctc
     6061 ttcatgggtg tgcgtggtct tttcaacgct cttgaaatgc cacttccagg tgactttgca
     6121 acttacacac aaatcttgac agatacagcc ttcatcatct tgccaggttt ggttgtgtgg
     6181 tcaaccttcc gtgtatttgg tggaaatcct gccgttggta tcgttcttgg tatgatgctt
     6241 gtctctggct cacttccaaa cgcttgggca gttgctcaag gtggtgaagt aacagcgatg
     6301 aacttctttg gtttcatccc tgttgttggt ttgcaaggtt ccgttcttcc agccttcatc
     6361 atcggggttg tcggagctaa atttgaaaaa gctgtccgca aggttgttcc agatgtcatt
     6421 gacctcttgg taacaccatt cgtgacactt ttggtcatgt ctatccttgg actctttgtc
     6481 attggaccag ttttccacgt tgttgaaaac tacatcctta ttgctacaaa agcgattctt
     6541 agcatgccat ttggtcttgg tggtttcttg attggtgggg ttcaccaatt gatcgtcgtg
     6601 tcaggtgtgc accacatctt caacttgctt gaagtgcaat tacttgctgc tgaccatgct
     6661 aacccattca acgctatcat cacagctgct atgacagctc aaggtgctgc tactgttgcg
     6721 gttggtgtta aaacaaaaaa tccaaaactg aaaacacttg ctttcccggc tgctctttct
     6781 gccttcctag gtattacaga gcctgctatc ttcggggtga acttgcgctt ccgtaaacca
     6841 ttcttccttt cattgattgc tggtgcaatc ggtggtggat tggcttctat ccttggactt
     6901 gctggtactg gtaatggtat caccatcatc cctggtacaa tgctttatgt tggtaacgga
     6961 caacttccac aataccttct tatggtagct gtatcatttg cccttggttt tgctcttact
     7021 tacatgtttg gttacgaaga tgaagtagac gcaactgcag ctgcaaaaca agctgaagtg
     7081 gctgaagaaa aagaagaagt tgcgccagca gctcttcaaa atgaaacact tgtaactcct
     7141 atcgtcggtg atgttgtcgc tcttgctgat gtcaatgacc cagtcttctc aagtggagct
     7201 atgggacaag gtatcgctgt gaaaccaagt caaggcgtgg tctatgcact agctgatgct
     7261 gaagtttcaa ttgcctttcc aacagggcac gcttttggtt tgaaaacaag aaatggtgct
     7321 gaagttttga ttcacgttgg tattgacact gtatctatga acggtgacgg ttttgaagca
     7381 aaagttgctc aaggtaataa ggtgaaagct ggcgatgttc ttggaacatt tgactcaaac
     7441 aaaatcgctg cagctggact tgatgataca acaatggtta tcgttacaaa tacagctgac
     7501 tacgcttcag tagctccagt cgcaacaggt tcagtttcga agggggatgc tgtgatcgaa
     7561 gtgaaaatct aatcaatcct ctctaatgtg aaaacgaaca aatgacatgt ttgttcgttt
     7621 ttattagata gtaagattta cagaggaaaa tctaaaaaat agagacattt agactttcga
     7681 agtatgctat aataaagaaa ataaaaacaa gaggtttatc atgacaaaat tatatggaag
     7741 cttggaagcg ggcggtacaa agtttgtctg tgctgtcggt gatgaaaact ttaacgttgt
     7801 agaaaaaaca caatttccaa caacaactcc aatcgaaaca atcgataaaa ccattgagtt
     7861 cttctcaaaa ttcgataacc ttgctggtct tgcagttggt tcatttgggc cgattgatat
     7921 tgacaaaaac tcaaaaactt atggctttat cacgacgact ccaaaaccaa actgggcaaa
     7981 tgtggacttg cttggtgccc ttcgtcgcgc cctaaacgtg ccaatgtact tcacaacaga
     8041 cgtaaacagc tctgcttacg gtgaaatggt tgctcgtaac aatgctggtg gtcgtatcga
     8101 aaacttggtt tactacacaa tcggtacagg tatcggtgca ggcgtcatcc aacgtggtga
     8161 gtttatcggt ggtgtgggtc accctgaaat gggtcattat tatgttgcta gacacccaat
     8221 ggatattgaa aaagagttta agggtgtttg tcctttccat aagggatgtc tagaaggtta
     8281 tgcagctggt acaagtttgg aagctcgtac aggtgtacgt ggggaaacta ttgaactcaa
     8341 caaccctgtt tgggatgttc aagcctacta tatcgctcaa gctgcggtta atgcgacagt
     8401 gactttccgc ccagacgtga ttgtctttgg tggaggggtc atggctcaac aacatatgct
     8461 ggaccgtgtc cgtgagaaat ttacatctct tcttaatggt tacctaccag taccagatgt
     8521 gcgtgactat atcgtgacgc cagcagtcgc aggaaatggt tctgccacac ttgggaactt
     8581 tgttcttgca aaagaagttt caaaataaag cacaaaatcc gcttgggaaa ccaaacggat
     8641 tttgtggttg aaaacaatat ttttgagcct ttccttaagt gattacggac ggtagcgact
     8701 ttcttcgaaa ttccatacct aaactttgag cctagtgtct caaagtttcc gaatacctga
     8761 aaccattaga tttcaggtgt ttctatcacg gcggaaaggc taggtaagtc ctcgcctcac
     8821 tatcgaaaaa tatgcataat ttccatacca aagtatattt agtatcaagc tatagttaat
     8881 ctacgcttta aaaatccact tgggaaacca aacggatttt ttacgtgtca aagcatttgc
     8941 cagaaaaagt caataatata ggtcagaccg taggtataaa ccacgagggt aaagggtttg
     9001 gtcagaatga tgatagtcat gtagcgcttg aaactcatct tggtcagggc ggccagcata
     9061 cagagaaagt cagctgggct aatgggccaa atcatcataa aaataaagaa gcggtcaaaa
     9121 cgattgccct tatctagcca gccgatgtac ttgtcgtagg tgcgcttgct gacgacagac
     9181 tggacaaagg cagctccgta taggcgcact agataaaaga taatggcaca gccaatcacg
     9241 atgccgatat agttgtagat agtcccgatg atgtgcccgt agataaagac cccagccacc
     9301 gaggtcaagg cccctggaat gatagggacg acagtctgta aaatctgtaa aaagataaag
     9361 agaggtggac cccagatgcc tgcctgctgg ataaaggcag agagggtttc cttggattgt
     9421 aaaatcccag cctgataagc ccaaatacag aaaatcaaac tcccaacccc accgacaatc
     9481 gatgagatat tgataactcg ctgcagaagg ggagaaacag ctttcatttg tttatcctgt
     9541 gacatgtaaa ctcctcatag atagtatatt tctactatac catattttgg aggagaaagg
     9601 gggaatgagt cttttttgat aaggaataga aagtaaatag cccttcgaga cttagaatag
     9661 gtctgtcaac taaaggttat atgcattcta ggtttcgaat aatcgcaatt ccataaattt
     9721 aaaagtattc taagtttcga atagcttatc tgagataatt ttgaggcatt cgcgctttcg
     9781 aatagtcatt ttccctaaag tttatatgta ttctgaagct agaaagacta aaatataaaa
     9841 gtagtaagag tctctctctg atggacgtaa tttatggcta gtatgaatcc tttgaattgt
     9901 tagttaattt taatattaat cagttctgaa ataattacta aatatagtta gatggtgagt
     9961 taattattgc taaatttcaa gttcaagtta gtcatccaga agtcttctct gggggacttg
    10021 tagttcaagc attttttagg atagttgtta atccactttt cgataaatgc gacttccttt
    10081 gtagttgttt tcttagttcc ctgaggtaac catctatgaa tgagcctgtt gtgattttca
    10141 ttagatccta tagtgatgtg cataatagat atagcagatt gaaactagaa tag
//
LOCUS       DAVBCG010000069        10100 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBCG010000069 DAVBCG010000000
VERSION     DAVBCG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.069"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            66..1508
                     /gene="gtfA"
                     /locus_tag="VQ495_001375"
     CDS             66..1508
                     /gene="gtfA"
                     /locus_tag="VQ495_001375"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2531326.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            1646..2839
                     /locus_tag="VQ495_001376"
     CDS             1646..2839
                     /locus_tag="VQ495_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2531327.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            3526..5505
                     /locus_tag="VQ495_001377"
     CDS             3526..5505
                     /locus_tag="VQ495_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2531328.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            5572..7068
                     /locus_tag="VQ495_001378"
     CDS             5572..7068
                     /locus_tag="VQ495_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531329.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            7068..7994
                     /locus_tag="VQ495_001379"
     CDS             7068..7994
                     /locus_tag="VQ495_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531330.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            8003..9070
                     /locus_tag="VQ495_001380"
     CDS             8003..9070
                     /locus_tag="VQ495_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531331.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            9081..10007
                     /locus_tag="VQ495_001381"
     CDS             9081..10007
                     /locus_tag="VQ495_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531332.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         2974 a         1794 c         2112 g         3220 t
ORIGIN      
        1 atgaatttga tttacgaagc cagatcatat gagacttggc ttcaattaga aaaaggagag
       61 taatgatgcc aattcaaaat aaaaccatgt tgattaccta ttctgatagc cttggaaata
      121 atcttaaaga cttatatgat aatttggaag agcattttgg agatgctatt ggaggagttc
      181 accttttacc atttttccca tcaacaggtg atcgtggatt tgcgccagtt gactacgacg
      241 aagtggactc agcttttggt gattgggagg atgttaagcg tttaggtgag aaatattatc
      301 ttatgtttga ttttatgatt aatcatattt ctcgtcaatc caagtattat aaggactatc
      361 aagaaaaaca tgaagccagt gaatttaaag atctcttttt aaactgggat aagttttggc
      421 cagaaaaccg tccgacacag tctgatgtag atttaattta caagcgtaag gatcgtgcac
      481 caaagcaaga gattgtgttt gaagatggtt cagtggaaca tttgtggaat acctttggtg
      541 aggagcagat tgatcttgat gtgaccaaag aagtaactat ggaatttatc cgtaagacca
      601 ttcagcactt ggcaagtaat gggtgtgatt tgattcgtct agatgccttt gcttatgcag
      661 tgaagaaatt ggatactaat gatttctttg tggaaccaga tatttgggat ttattggaca
      721 aagttcgaga tatcgctgct gagtatggga cagagatttt acctgagatt catgaacact
      781 attcgattca gtttaaaata gcagaccatg attactatgt ttatgatttt actcttccaa
      841 tggtgacact ttatactctt tacagttcca gaacagagcg tttggctaag tggttgaaga
      901 tgagcccgat gaagcaattt acgacgctag atacccatga tgggattgga gtagtagatg
      961 tcaaggatat cctgaccgat gaggagattg actatgcttc aaatgaactc tataaggttg
     1021 gagccaatgt caaacgtaag tactctagtg ccgagtataa caacttagat atctaccaaa
     1081 tcaattcaac ctactattca gcgcttggag atgatgatgt caagtatttt ctcgctcgtc
     1141 taattcaagc ttttgcccca ggtattcctc aggtttacta tgtgggtcta ttagcaggca
     1201 agaatgactt gaaattatta gaagaaacta aagaaggtcg aaatattaat cgtcattact
     1261 atagcaacga ggaaatagca agagaagtgc aacgacctgt tgtgaaggcc cttctcaatc
     1321 tattttcttt ccgtaaccgt tcagaagcct ttgatctaga agggactact gagatagaga
     1381 caccaacagc tcacagcatt gtaatcaaac gtcaaaataa agataagtcc gtaacagcag
     1441 tagcagaaat tgatttgcaa aatcagactt atcgggtaat tgagaatgga gttgaagtat
     1501 cattttgaag ccttgatatg gtggataaaa tagggttttt attttggaaa acgtttcctt
     1561 tgttttcaaa ttgctaaaaa agtggtacaa tataggatag cttactatta tctgaatcag
     1621 ctgatttgga gagaaaggat tcattttgaa atcaataggc tttattgaaa agctgaaggg
     1681 gttgtctagt aaagagctga ttttattggg aattatccta agtatctttt taccctttta
     1741 tctttttgta gttgtactct gtttatatat tatcagtttg atttttacag gagacatgaa
     1801 aagtattctt cagaaaatgg gggagcatcc gatgctgctt ctttttctta gctatagtac
     1861 tgttatatcc attcttgcac aaaattggat gggtcttgtg gcttcagtag gaatgtttct
     1921 atttactatt ttctttttgc actatcagtc gattttatcc cataaattct ttcgattgat
     1981 tttgcagttc gtcttgtttg gtagtgtctt gtcagctgct tttgccagtt tagaacattt
     2041 ccaaattgtg aagaaattta actatgcttt tctttcaccc aatatgcagg tgtggcatca
     2101 gaaccgggca gaagtgacct tctttaatcc taattattat ggaattattt gttgtttctg
     2161 tattatgatt gctttctatc tgtttacaac gaccaagttg aattggttga aagtattctg
     2221 tgtgattgca ggctttgtta atctctttgg tttgaacttt actcaaaatc gaactgcctt
     2281 tcctgctatt atcgctggag caattatcta tctctttacg actattaaaa actggaaggc
     2341 cttttggctt agtattgggg tcttcgcgat tggtttgagt ttcctctttt ctagtgattt
     2401 gggagttcga atgggtactt tagactcttc tatggaagaa cgcatttcta tctgggatgc
     2461 tgggatggcc ttgtttaagc aaaatccttt ttggggtgaa gggccattga cctatatgca
     2521 ctcttatcct cggatacatg ctccttatca tgaacatgcc cacagtcttt atattgatac
     2581 gattctgagt tacggaattg tgggtaccat tttattagtt ttgtcttctg ttgctcctgt
     2641 tcgcttgatg atggatatga gtcaggagtc ggggaaacgt ccgattatcg gcctttatct
     2701 atctttcctt acagtggttg ctgtgcacgg aatttttgac ttggctctct tctggattca
     2761 gtcaggcttt attttcttgc tagttatgtg cagtattcca ttggagcatc gaatgttggt
     2821 atcggacatg acggattaag ttttataagt ttgaaatctt ctaccttggg taggagattt
     2881 ttttatacga tgggcatgtc gtgcatctga ggtgtaagtc ctctgtaggg atccactacc
     2941 ggtgaataca atagcgctcc ccaagcctga ctatcgtgag gtagcggatt aaaatggtct
     3001 ggggatagat cgttttaagt ctgacgcagg aaataagaat tgtcagagaa agcaatagtg
     3061 aaatcgtagg ctatgaatag aaacgtgata gcgaggagca tatagtggat aaggtagcga
     3121 gaaattttaa agtataaaag atagtcttaa tctatatatg taaatcacaa aagtaattga
     3181 attcggaata aggttggtgt gaaaagacat agtaaactga atcttgaata ctagaatgat
     3241 acttctacaa tattgttata atttaatttg aattccaaaa tcacattgtt ccgtccgtat
     3301 ttcattttac tgtatgtctt cctaaactcc aaagtttctg cgtcaaattt cctattttca
     3361 ctgtagtctt tgacacttcg aacgacaatt tgaatcatat attcaaaaaa aaatgaaaaa
     3421 tttgtgccaa caagcctaaa tattctgaaa atttgtttga tttttaatgg ataatgtgat
     3481 ataatggttg tcaatctata attcacataa acagaggagt agaaaatgaa aaaaaatcgt
     3541 gtatttgcta cagcaggtct tgttttatta gcagcaggtg tacttgcagc atgcagttct
     3601 tcaaaatcat ctgattcatc agcccctaaa gcttatggct atgtttatac agcagaccca
     3661 gaaaccttgg actacctgat ttcaagtaaa aatagtacaa cagtagtgac ttcaaatggg
     3721 attgatggtt tattcactaa cgataattac ggtaatcttg ctcctgcagt tgcagaggat
     3781 tgggaagtct ctaaggatgg tttgacctac acttataaga ttcgtaaagg ggttaaatgg
     3841 tttacctctg atggagaaga atatgcagag gtgacggcta aagatttcgt gaacggttta
     3901 aaacacgcag cagataaaaa atcagaagct atgtatttag ctgaaaattc ggttaaaggc
     3961 ttggcagatt atctatcagg aacttcaaca gatttttcaa cagttggtgt caaggcggtt
     4021 gatgattata cgttacaata cactttgaac cagcctgaac cgttctggaa ctctaagttg
     4081 acctattcta ttttctggcc tctgaatgaa gaattcgaaa catcaaaagg aagcgatttt
     4141 gctaaaccaa cagatccgac atccttgctt tataatggtc cattcttgtt gaaagggttg
     4201 actgcaaaat cttctgtaga gtttgtaaaa aatgagcaat attgggataa agaaaatgtc
     4261 cacctagata ctatcaatct agcttactat gatggatcag atcaggagtc gctagagcgt
     4321 aacttcacta gtggagctta tagttatgcc cgtctttacc ctaccagctc caactattct
     4381 aaggttgcag aagaatacaa ggacaatatc tattacacac aatcaggctc tgggattgct
     4441 ggtctgggtg tgaatattga tcgccaaagt tacaactata cttctaaaac tacagattca
     4501 gagaaagtag ctactaagaa ggcattgctt aacaaagatt tccgtcaagc cttgaatttt
     4561 gctcttgatc gctcagctta ctcagctcaa atcaatggta aagatggagc agctttagca
     4621 gttcgtaatt tatttgtaaa accagacttt gtttcagctg gtgagaagac ctttggtgat
     4681 ttagtcgctg ctcaacttcc tgcttatggt gatgagtgga aaggtgtgaa tttagctgat
     4741 gggcaggatg gtttattcaa tgctgacaag gccaaggcag agtttgcgaa agctaagaaa
     4801 gctttagaag cagacggcgt tcagtttcct attcatctgg acgttccagt agaccaagca
     4861 tcaaaaaact acatatctcg tattcagtcc tttaaacaat ctgtagaaac agttcttggt
     4921 gttgaaaatg tcgttgttga tattcaacaa atgacaagtg atgaattcct taatattact
     4981 tactatgctg ccaatgcttc atctgaggat tgggatgtat cgggaggagt ttcatggggg
     5041 ccagactatc aagacccatc tacttacctg gatattttaa aaacaactag cagtgaaact
     5101 acaaaaacat atttaggatt tgataatcca aatagccctt cagtagttca agttggtttg
     5161 aaagaatacg ataaattagt tgatgaagct gccaaagaga caagcgactt gaatgtccgt
     5221 tatgaaaaat atgcagcggc tcaagcatgg ttgacagata gttcactctt tattcctgct
     5281 atggcttctt ctggtgcagc accagtgctt tcacgaattg ttccatttac tggagcttct
     5341 gcgcaaacag gctctaaggg gtcagatgtt tacttcaaat atttgaaatt acaagataaa
     5401 gtggtgacta aggaagagta tgaaaaagct cgtgaaaaat ggttgaaaga aaaagctgaa
     5461 tcaaatgaga aagctcaaaa agaattggca agtcatgtga agtaaataat ttctaataga
     5521 actttctctc catgaggaga aggttctttt gggattttta aaggaaataa tatgaaaaaa
     5581 tatattttta tgcgtgtttt gcggtcattg gtttcgattt tcttagtaac gactttgacc
     5641 tacacgatta tctatacctt ggttcctcga aaattgattt tcaagcagga tcctaactat
     5701 aataaaattg caacaacggc tgataaacgt gataactatg aaaatactgt gtttgagcgt
     5761 atgggctaca ttgagtatta cgatactaaa gagttgcaag aaaaggcaag tagcatggat
     5821 tcttctgtaa cagtagaagc aaatgcgacc aataaagcta tttatgaaaa gtacatcaat
     5881 caattaggtc atggttggac tttgggagaa tttactgaaa gtggtcaatt ctatgctact
     5941 cgtgaaattc caatttttga acgtgttttt cacttctatg ctaacttgat tgacattgac
     6001 catacaaata aaatccaaga ccctgaaaat ccagacttga aacgctacct tcgttttgaa
     6061 aatgatccag ctatcggatg gtcattggtc ggttcaggaa ctaaacataa atatctcttg
     6121 tactttaaca gtcagttccc atttgttcat caaaactttg tgaacttgaa tttaggtgac
     6181 tcttacccaa cctatgctaa tacaccagtt cttcaggtta ttactcaagg tcaaggacaa
     6241 accaaaactg cccaagttca gttcccaaca ggtaagaaaa cgtcttctgt aaatatttac
     6301 tcaagaacct acaagtcacc tagtcaggct gactctcgtg aagtagctag ctatgggaaa
     6361 gatgatcctt atacagcgac tgaaagtaat taccaatatc catctatgat tgtcagctct
     6421 gctattactg gtttgattgg tttggttctt gcctatgctc ttgccgtgcc acttggttca
     6481 gccatggctc gtttcaagaa cacttggatt gatagcctct caacaggggc tttgaccttc
     6541 ttgcttgctc ttccaacgat tgccttggtt tacatcgttc gattgattgg atcatctatt
     6601 gcccttccag attcattccc tatcttggga gctggagatt ggcgttctta cgttttacca
     6661 gcagtcatcc ttggtttgtt gggtgctcct ggtacagcca tttggattcg tcgttacatg
     6721 attgacttgc aatctcaaga ctttgttcgt ttcgctcgtg caaaaggttt gtctgaaaaa
     6781 gaaatttcaa acaaacacat ctttaaaaat gccatggttc cgctggtttc aggaattcct
     6841 ggtgccatca ttggggttat cggtggtgca acccttactg aaacagtctt cgccttccca
     6901 ggtatgggta aaatgttgat tgactctgta aaagcatcta ataactctat ggtcgttggt
     6961 cttgtcttca tctttacatg tatttctatc ttctcacgtc ttttgggaga tatttggatg
     7021 actattattg acccacgtat taaattgact gagaaaggag gcaaataatg tctacaatcg
     7081 ataaagaaaa atttcagttt gtaaaacgtg acgattttgc ctctgaaact attgatgcgc
     7141 cagcatattc ttactggaaa tcagtgttta aacaatttat gaagaaaaaa tcaactgtag
     7201 tcatgttggg aatcttggta gccatcattt tgataagttt catctaccca atgttttcta
     7261 agtttgattt caatgatgtc agcaaggtaa acgactttag tattcgttat atcaagccaa
     7321 atgcggagca ttggttcggt actgacagta acggtaaatc gctctttgac ggtgtctggt
     7381 tcggagctcg taactccatc ctcatttctg tgattgcgac agtgattaac ttggttatcg
     7441 gtgtttttgt cggtggtatt tggggtattt caaaatcagt tgaccgtgtc atgatggaag
     7501 tttacaacgt catctcaaac atcccacctc ttttgattgt tattgtcttg acttactcaa
     7561 tcggagctgg attctggaat ctgatttttg ccatgagcgt aacaacatgg attggtatcg
     7621 ccttcatgat ccgtgtgcaa atcttgcgct atcgtgactt ggaatacaac ttggcatcac
     7681 gtactttggg aacaccaacc ttgaagattg ttgccaaaaa tatcatgcct caattggtat
     7741 ctgttattgt gacaaccatg actcaaatgc ttccaagctt tatctcatac gaagccttct
     7801 tgtctttctt cggtcttgga ttaccgatta cagtgccaag tttgggtcgt ttgatttcgg
     7861 attattcaca aaacgtaaca accaatgctt acttgttctg gattccattg acaacccttg
     7921 tcttggtatc cttgtccctt ttcgtagttg gtcaaaactt agcggatgct agtgatccac
     7981 gtacacatag ataggagtag aaatgacaaa agaaaaaaat gtaattttga ctgctcgcga
     8041 tattgtcgtg gaatttgacg ttcgtgacaa agtattgaca gccattcgcg gcgtttccct
     8101 tgaactcgtt gaaggagaag tattagcctt ggtaggtgag tcaggatcag gtaaatctgt
     8161 tttgacaaag accttcacag gtatgctcga agaaaatggt cgcattgccc aaggtagtat
     8221 tgactaccgt ggtcaggatt tgacagcttt atcttctcac aaggattggg aacaaattcg
     8281 tggtgctaag attgcgacta tcttccagga cccaatgact agtttggacc ccattaaaac
     8341 aattggtagt cagattacag aagttattgt aaaacaccaa ggaaaaacag ctaaagaagc
     8401 gaaagaattg gccattgact acatgaataa ggttggcatt ccagacgcag atagacgttt
     8461 taatgaatac ccattccaat attctggagg aatgcgtcaa cgtatcgtta ttgctattgc
     8521 ccttgcctgc cgacctgatg tcttgatctg tgatgagcca acaactgcct tggatgtaac
     8581 tattcaagct cagattattg atttgctaaa atctttacaa aacgagtatc atttcacaac
     8641 aatctttatt acccacgacc ttggtgtggt ggcaagtatt gcggataagg tagcggttat
     8701 gtatgcagga gaaatcgttg agtatggaac ggttgaggaa gtcttctatg accctcgcca
     8761 tccatataca tggagtctct tgtctagctt gcctcagctt gctgatgata aaggggatct
     8821 ttactcaatc ccaggaacac ctccgtcact ttatactgac ctgaaagggg atgcttttgc
     8881 cttgcgttct gactacgcaa tgcagattga cttcgaacaa aaagctcctc aattctcagt
     8941 atcagagaca cattgggcta aaacttggct tcttcatgag gatgctccaa aagtagaaaa
     9001 accagctgtg attgcaaatc tccatgataa gattcgtgaa aaaatgggat ttgcccatct
     9061 ggctgactag gaggaaggaa atgtctgaaa aattagtaga aatcaaagat ttagaaattt
     9121 ccttcggtga aggaagtaag aagtttgtcg cggttaaaaa tgctaacttc tttatcaaca
     9181 agggagaaac tttctcgctt gtaggtgagt ccggtagtgg gaaaacaact attggtcgtg
     9241 ctatcatcgg tctaaatgat acaagtaatg gagatatcat ttttgatggt caaaagatta
     9301 atggtaagaa atcgcgtgaa caagctgcgg aattgattcg tcgaatccag atgattttcc
     9361 aagaccctgc tgcaagtttg aatgaacgtg cgactgttga ttatattatt tctgaaggtc
     9421 tttacaatca ccgtttattt aaggatgaag aagaacgtaa agagaaagtt caaaatatta
     9481 tccgtgaagt aggtcttctt gctgagcact tgactcgtta ccctcatgaa ttctcaggcg
     9541 gtcaacgtca acgtatcggt attgcccgtg ccttggtcat gcaaccagac tttgttattg
     9601 cagatgagcc aatttcagcc ttggacgttt ctgtacgtgc ccaagtcttg aacttgctca
     9661 aaaaattcca aaaagagctc ggcctgacct atctcttcat cgcccatgac ttgtcggttg
     9721 ttcgctttat ttcagatcgt atcgcagtta tttacaaggg tgttattgta gaggttgcag
     9781 aaacagaaga attgtttaac aatccaattc acccatatac tcaagccttg ctttcagcgg
     9841 taccaatccc agatccaatc ttggaacgta agaaggtctt gaaggtttac gacccaagtc
     9901 aacacgacta tgagactgat aagccatcta tggtagaaat ccgtccaggt cactatgttt
     9961 gggcgaacca agccgaattg gcacgttatc aaaaaggact aaactaataa tggttttata
    10021 atttccatgt caatttttat gggaattata aaactttttt gttggactga tttgaaaaac
    10081 tttgaaagct tcctgaaaga
//
LOCUS       DAVBCG010000070        10030 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBCG010000070 DAVBCG010000000
VERSION     DAVBCG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.070"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(28..1731)
                     /gene="ilvD"
                     /locus_tag="VQ495_001382"
     CDS             complement(28..1731)
                     /gene="ilvD"
                     /locus_tag="VQ495_001382"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2531333.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1964..2896)
                     /locus_tag="VQ495_001383"
     CDS             complement(1964..2896)
                     /locus_tag="VQ495_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2531334.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2893..3750)
                     /locus_tag="VQ495_001384"
     CDS             complement(2893..3750)
                     /locus_tag="VQ495_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2531335.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3754..5100)
                     /locus_tag="VQ495_001385"
     CDS             complement(3754..5100)
                     /locus_tag="VQ495_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2531336.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(5113..5397)
                     /locus_tag="VQ495_001386"
     CDS             complement(5113..5397)
                     /locus_tag="VQ495_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2531337.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5401..7431)
                     /locus_tag="VQ495_001387"
     CDS             complement(5401..7431)
                     /locus_tag="VQ495_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2531338.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7623..8630
                     /locus_tag="VQ495_001388"
     CDS             7623..8630
                     /locus_tag="VQ495_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2531339.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8633..8833
                     /locus_tag="VQ495_001389"
     CDS             8633..8833
                     /locus_tag="VQ495_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2531340.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            8985..9167
                     /gene="rpmF"
                     /locus_tag="VQ495_001390"
     CDS             8985..9167
                     /gene="rpmF"
                     /locus_tag="VQ495_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2531341.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            9183..9332
                     /gene="rpmG"
                     /locus_tag="VQ495_001391"
     CDS             9183..9332
                     /gene="rpmG"
                     /locus_tag="VQ495_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2531342.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9824..>10030)
                     /locus_tag="VQ495_001392"
     CDS             complement(9824..>10030)
                     /locus_tag="VQ495_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2531343.1"
                     /translation="YLNESGSMATGWVKDKGLWYYLNESGSMATGWVTVSGKWYYTYN
                     SGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3248 a         2133 c         1620 g         3029 t
ORIGIN      
        1 gatttgaaga gattttcgag tatgagttta ttttttacct gacttgtcca tattccagaa
       61 gtctgtcacg gctccgcgtg aagcagatga tacgatgtgg gcatatttac cgaggacacc
      121 acggctgtaa agtggtggca aggttgtttc tgccttgcgt ttttcaagtt cttcttcgga
      181 tacggccatg gaaatttctt tggtatcttg gtcaaccgta acgatatcgc cggtacggag
      241 ataggcaatt ggtccaccat cctgagcttc aggagcgata tgtccaacaa ccagaccata
      301 agtaccacca gagaaacggc cgtccgtcaa gagggccacc ttatctccct gacctttacc
      361 aacaatcatt gaagaaagtg acagcatctc aggcatacca ggaccacctt taggtccaac
      421 aaaacgaaca acgactacat cgccatcaac gatttcatct gtcagaacgg cctgaatcgc
      481 atcttcttct gagtcaaaga ccttagctgg cccaacgtga cgacgcactt taacacctga
      541 taccttggca actgcaccgt caggagcaag gttcccgttc aagatgataa gcggaccatc
      601 cgcacgtttt ggattttcaa gtggcatgat aactttttgg cctggagtca agtctgcaaa
      661 gtcagccaag ttttcagcta cagtcttacc agtacatgtg atgcgatctc cgtgaaggaa
      721 accatttgcc aacaagtact tcataaccgc agggacacca ccgacttcgt agaggtcttg
      781 gaagacatac tgaccagatg gtttcaagtc ggccaagtga ggcacacgtt cttgaatcgt
      841 attgaagtcc tcaagtgaca agtcaacatt tgcggcatgg gcaatggcga gcaagtgaag
      901 agtggcgttt gtagaaccac cgagagccat cgttacagtg atagcatctt caaaggcttc
      961 acgagtcaag atatctgatg gtttgagacc aagttccaac atcttaacaa cagcacggcc
     1021 tgctgcttcg atatcttctt tcttatcagc tgattcagct gggtgagagg atgaccctgg
     1081 caaactcatc cctagaactt cgatagcagt tgccatggta ttagcagtat acataccacc
     1141 acagccacca gggccagggc aggcattaca ttcaagacgt ttcacgtcct cagctgtcat
     1201 gtcaccgtgg ttccattttc cgataccttc aaagacagaa accaagtcga tatctttacc
     1261 atcaagattt cccggtgcaa tagttccacc ataggcgaaa atagctggga tatccatatt
     1321 agcaatagca atcatagatc caggcatgtt cttgtcacag ccaccgatag cgacgaaggc
     1381 atccacgttg tgaccactca tagccgcctc gatggagtcc gcgatgatgt cacgagatgt
     1441 tagagagaaa cgcataccag gcgttcccat agcgatcccg tccgctacgg taatggttcc
     1501 aaactgtaca ggccaagcgc ctgcagattt gacaccttct ttagccagtt tcccgaaatc
     1561 atgcaagtga atgttacatg gtgtattttc cgcccaagtc gaaatcactc ccacaatcga
     1621 tgtttcaaag tccttatctg tcataccagt cgcacgaagc atagcacggt taggtgattt
     1681 aaccatgctg tcatagatgc tactgcggtg acgtttatct aattcagtca tcttatccct
     1741 cccatttcag tttttactat tatagcacaa ttttcgcatg aagaacagaa taaaattctt
     1801 gaattttcag aaaattctat acacatgtga aatatttaaa attaaaaaca acaaagcgga
     1861 ttagtgcact ttctgatgac cagaatatgc tttttaatcc gctttcttta aataacgtac
     1921 tgtaattttt acagaaattc tttcaaataa gtgtatttaa catctatctt gcattataaa
     1981 tttctagaac cttctctttt atattcgatt cactcaaacc atactcatta agaagataat
     2041 ccattttccc tacttgaccg aatctttctt gaacacccat ccgatgaatt tttgttattc
     2101 catcatcaga gaataattca cataaagcac tgccaattcc acctatctga ttgtggtttt
     2161 ctacagtaaa tatagttttt ccacttaaca ttgtttttat ctgttctggt atcggtttga
     2221 ttctaaataa atctatcaca cctactgaat aacctaattt agacagttca tctgcaactc
     2281 gaatacttgg agcaaccatt atgccagaag caacgattac aagatcttca ccatgcctta
     2341 actcaatgta gcctttagaa aaatcttctc caccttgata cacaggaact ggagcttttc
     2401 taattgttcg aatatatttt agtcctttta agtctaatgt ctggttcaat atttcacgaa
     2461 attggatatc atcagttgct tcgaaaatga ttgatttagg aattaaacgt aacaatccaa
     2521 tttcttcaaa tggcatatgt gttccaccat tcatctctgc cgttactcct gcatctgatc
     2581 caatcacagt ggcatccaat tgtgcgtatc caagagaaat aaataattga tcaaatactc
     2641 ttcgtgaagc aaaaggacca aatgtatgaa gataaggtct aaacccctga atagacaagc
     2701 ctgctgcaag gccgaccatt tctgcttcca taatcccaac attcacataa cggtctccaa
     2761 agtccttttc aagattatta gtagccatcg aacttgacaa atcggcttct aagactacta
     2821 tatcagaatc actttgatta gcctctaaaa ggaagtctct atatacatgc cgtaattctt
     2881 tcgtacttct catcattctg tttcctccaa ttcctgactt aatctttcta caactgaagt
     2941 taacatttgt ttctcctcta cagtagggcg aagatgatga ttggatttca tttcttccag
     3001 ctcttgaacc ccttgacctt taatagtatc taatacaatg cacttaggtg atgaattatt
     3061 tgactgtttt aattggacaa tcccttcata aatttctcta atatctgaac ccttgaccct
     3121 aatggattca aatccaaatg ctgaaaattt ttctacgaaa tcacctggat tacaaatatc
     3181 ctttgtaaaa ccatctaatt gttttttgtt atcatcaaca aatacaatta agttggataa
     3241 ctgttgatga gaagcaaact gtatagcctc ccaacattgt ccctcattta actcaccatc
     3301 tccaacaata gcgtaagtat aaaagggact ctttcttatt ctctgaccat atgcaagtcc
     3361 agttgcaaca ctaattcctt gtcctaaaga gcccgttgtc atatctatgc ctggcgttag
     3421 atttctatca ggatgagacg gtaatttggt tccatttgta tttaaagaat ataagaattc
     3481 tttgtcaaag aaaccattca aatagagtgt actgtataga gctggtcctc cgtgaccttt
     3541 tgataatatg aaataatctc tatctcgtgc tgcaaatatt tctggagtca ttggcattat
     3601 ttcaccataa agcaccgcta aaacttctac gatagacaga cttcctccgt aatgtccgaa
     3661 tccaagatga ttcaatgttc taagagtatt taatcggatg ttagtcgcaa attttcttaa
     3721 cccatcttct ctatttttac ttaaaatcat cccttattcc tccgttgcag atggcttttt
     3781 aataaaggat actccaaaca taactgctag aataagaaca agaccaatca caatgcctgc
     3841 ttgtgagcca aattgattta acattcctaa aataattcct gatagaccaa aatctgcatc
     3901 tgagaaagtt gatccttgga aaccaagtcc tcccaaaact ggcattaaaa agactggaag
     3961 aaaactgatt aaaatacctt gtaaaaatgc tccaatagtg gctccacgaa caccaccaga
     4021 tgcattccca atgacacctg cagtcgctcc acagaagaaa tgaggcacaa cacctggtaa
     4081 gataacaacc gttcctgaag caatcataat taccatactt actaaaccac caacaaaact
     4141 agagataaat ccaattagaa ctgcattggg tgcataagta taaacaatcg gacaatccaa
     4201 agcaggtttt gaattaggta caagacgctc tgaaatacct ttaaaggctg gaacaatttc
     4261 gcccaaaata aggcgaacac ctgctaaaat aacaaatacc cctgctgcaa attgacctgc
     4321 taattgtaaa gcataaacta gaccacttgt accactactg atttcttttt ctatatattc
     4381 tgaccctgca aagatagcta caataatgta aataactgcc atggataaag taatactaac
     4441 agtactatca cgtaaaaaag ctaaactctt tggaaattta atgtcctctg ttgattttga
     4501 tttgtcaccg ataaggctac cagtaaaacc actcaaccaa tatcccaaag aactgaaatg
     4561 acctaaagct accttgtcat ttccagttaa ttgaaccata tatttttgca caaatgctgg
     4621 ggaaatactc ataataatac cgagtgctaa tcctcctagt aagatgagag gcaagctagt
     4681 aaagccagca actgataaaa tgaccgcaat catacatgcc atatatagag tgtggtgccc
     4741 tgttaaaaaa atatatttaa atcgagtaaa acgagcgatt aagatattga acaccatgcc
     4801 tgcaaacata atcattgcag tagctgagcc atatgttgtt aaagctacag ctacaattgc
     4861 ttcattattc ggcacaacgc cagataaatg aaaagcatgc tcaaacatgg taccaaatgg
     4921 atttaaagaa ttttgtacaa ttcctgcacc accagataca actaagaaac caacaaaggt
     4981 cttaattcca cctttaataa tatcaggtaa tttcttcttc tgaagaacta atcctaagat
     5041 tgcaattaaa gctactaaaa tagctggtgt actaacaata tccaatatga acttcatcat
     5101 gacgctagcc tcctatataa gtcctttttc ttcacaaagt ttagtaatta attctcgtag
     5161 ttcatccata tcaataatac tatttaagat acgaacatct ccaagatgac tagctgaatc
     5221 agctagatca cgaccaacaa tccaaatatc agctgcattt ggatctgctc cacctaaatc
     5281 ataatgttca acttctacat ccgaaacatt caaatcactc aatacagatt caatattcat
     5341 ctgtaccata aaacttgaac ctaatcctga accacaagct gtaccaattt ttaacattat
     5401 ctaatcctcc tgtttaatta tcattttaat gtcatcatag ttttttgatg atattaaagt
     5461 ttgaacatga tttttatctc ttaaaattgt tgttaaatgt gacaaagcct ttaaatgact
     5521 ctcattatca atggctgcaa tacaaatcaa caatcttacc tcttgttctg gattatccaa
     5581 taaataaatc ggttcttcca aaactaacat tgacattcct atttcattca caccttcatc
     5641 tggccgagcg tgaggaattg ctactccctt ccctaaatta ataaaaggtc caaactcttc
     5701 tactttttga atcattgcct cagggtagtt ctcagttatc ttatcttgat ccaaaagcgg
     5761 tttagctgct aaacgaatcg cctccttcca tcctaatttt tgcgaactaa cctgataggt
     5821 ttctttggta ataagttgtt ctagcactgg tacaatttcc tttctatcat ttttttggta
     5881 aagataattc tttaacgcca atcttaattc caattcttgt gtaataattc catatctttt
     5941 gacaatattc aggatttgtt caatctcaaa atctccatac tctaaattcg gaaaatcttt
     6001 taacactagt tctactagtt gtattgcttg ctcttcagtc atcataaccg aaactagata
     6061 atttggcttt tctgtctcca cctttatggt agaaaaaacc atatcatagt cactactagc
     6121 tttcacctgt aaatcatcaa tctttgaggt tcctataaac tcaatttgag gaaataatgc
     6181 taatagattc tcttttaaca tcaatgaaga actaacacca ttaggacaaa tgattgctgc
     6241 tttataccat ttttgaggca aagtatctgc tttctttaaa taacctccga aatggataac
     6301 aaaatatgct gtttcactat caggtatggg attgtcaata gcgtccatca agggcatcaa
     6361 agaatctttg actaattcaa ataaatcagg ataatgttct ttaacatgca atacatattc
     6421 atttgaacta ggtaggccga actttaatct atagtaagcc ggtataaggt ggcggcgaag
     6481 attttctctc aatccttccc tttgtttaaa atgtaacaaa gaaatatctt ccattctact
     6541 tataatagcc tctgttaatt gattaaagta aaccggagca acatctactt caccttcaaa
     6601 gcaacttgat aataaaacgg tgatatagcg ataatcatcc tcagaaaaca ccgtatctat
     6661 aattcccaaa tcaaccactg tatccaataa aatagtggtt atatcttgaa taacaggaga
     6721 tactaatgtc tctgaaagac atactctttc aacatccctt tgatacctac acagaatgaa
     6781 tactaaaccg aaaaggtaaa cttttaattg attaacaata ggtactagct gtagcttctc
     6841 ataataatct ttaactacct gatcaatcaa atcataagtt aatgaatacc cccaactgga
     6901 taaaacataa tccaaacccc aaatccctat ggaggattcc agcaactcac taaccatttg
     6961 aaaagctaag cggtgcttat tccactctga accgtgtaaa gtataacctt ttgctctact
     7021 gtaccctagc tccaaatcat tatctaacat aatctttctt aatgattgaa tatcagataa
     7081 ggttgtattc ttacttactt tcaaaaagtc ttggtaatga ctattcgata taaaatctaa
     7141 tcggcaaaaa gtgtaaagat agattaaagc taagcgagtc gactttggta aaaccaattc
     7201 atccgactta ataatatctg tcaaagactg cttcgtacga tttgataaac tatagcgacc
     7261 ttgcttttta tccagcacta tccctttatt agctagataa ggcactaaat aatctattcc
     7321 ttctttgact tcctttatag gtaagctcac cttaacagat aattcatata atgatagctc
     7381 acaatgatcc atcaaagtca tcaaaataac tagtgctcta taatcaaaca tttctcttcc
     7441 tttctaacta caattatagt gtaaaaatta taagaataaa agagtatttg agcacaatat
     7501 aatctcacct tttaaaagaa attattgggc tgaaagtttt tagtgattat ttttcaactt
     7561 tctattgact ttataaatta aagtgatatc atttagatat caaataacag gaggtcattc
     7621 ttatgactga aaaactaatc aattcaaaac caaatggtgt attcgcattg attctcattg
     7681 agttgacaat cgtacttggt atctttatat ttataatggg ggttggttcg gaaaacattt
     7741 ttggaattat tatcggacct ttactaatcg taattgcagg tctagctcat gctggtttaa
     7801 aagttgtcaa acctcaagaa gctctggttc tgacactctt tggtaactat acaggtacca
     7861 tcaaagaacc tggcttttac tttgtcaatc ccttcagcgt agcagtcaac cctgcaaacc
     7921 acactcgact tggacaaagt ggtgatgtta gcacaaaatc tcctttttta ggagctaaat
     7981 catcaaatga caatgatgta aatcttgaaa ttggcaagaa acagatttcc ctcaaagtca
     8041 tgaccttgag caattctcgt caaaaaatca atgattgctt aggaaaccct gtagaaatcg
     8101 gtatcgcggt aacttggaga gttgtcgata ccgctaaggc agtcttcaac gttgataact
     8161 acaaagaata tctttcattg caatgtgata gcgccctccg taatattgtc cgcatctatc
     8221 cttacgatgt gtctcctaat gtggatacta cgggtgatgg gcaagcagat gaaggtagtc
     8281 tccgtggctc tagcgaaatt gttgctaacc gtattcgtga agaaatccaa agtcgtgttg
     8341 aggatgctgg cttggaaatc cttgaagcac gtatcactta cctagcttat gctccagaaa
     8401 ttgctgccgt tatgcttcaa cgccaacaag catctgccat tattgatgca cggaagatga
     8461 ttgtagatgg tgctgtagga atggttgaaa tggcactaga acggctcaat gaaggggaat
     8521 tggtagaact tgacgaagaa cgaaaagctg ccatggtttc aaatctcctt gtcgttcttt
     8581 gtggcaatca tgatgcacaa ccaattgtca acacaggaag cctttactaa agatggctga
     8641 agctaaaaaa aagcagatcc cccttcgact ctcaacaaag ttatacgctg cactcgcatc
     8701 atgggcagaa gacgactttc gttcagtcaa tgggcaaatc gaatatctcc tcacagagtg
     8761 tgtcaagcaa cggaagaaag acggaaaata cgtatcagaa accattgacg aaccatttga
     8821 gattgatatt taacaccttc tcctgtctgc cgtgtcggat aggagaattt tttcatcttt
     8881 ttttgtcaag taactttact ttacaaaaaa aatgtgttat cctagtatgg ttgatgaaaa
     8941 tcagtagatt gaatcgaata taaataccta aaggagaaat caaaatggca gtacctgcac
     9001 gtcgcacttc aaaagcgaag aaaaacaaac gtcgtacaca ctacaaagta acagctccat
     9061 ctgtaaactt tgacgaaact actggagatt actcacgttc tcaccgtgta tcacttaaag
     9121 gatactacaa aggacgtaaa atcactaaag ctgcatcagc tgaataatag aagggagata
     9181 ccatgcgcgt aaatattaca cttgaacaca aagaatctgg tgaacgcttg taccttactt
     9241 ctaaaaacaa acgtaacact ccagaccgcc ttcaattgaa gaaatactca ccaaaacttc
     9301 gcaaacacgt tgtgtttaca gaggttaaat aagcattcaa tacgaatcaa tacagaagaa
     9361 aaacgcttga tcagcgtttt ttctttttgt taatttcact acattgtaag ggaaatcatt
     9421 cgaatgacta catttttcac tacctttttt gaaataagaa tacggattcg gacaattaaa
     9481 agcaagagga actcaaatgt aagttggggt tccttttttg tatatttttc aaataacgcc
     9541 tctacactat ttgtagcaaa ttcaccaact acagttgtat cttagttaaa ataagttaga
     9601 atatgtaagt gagtaccaga tataccaaga catcgtcacc atctaaggta tattcaaaat
     9661 acaaaagttg accaactaga tttctgaata tccttatata tccattctta aaattggttt
     9721 aaatagcgta gtcttttaaa ctagttttga gaatccaaaa aatcttccta catatgtaag
     9781 aagatttttt agttcagaat gattagattt agctaatgga tacctatcct acccactcac
     9841 cgttagcatt gactcgatag ccatcgggtg tagtcgtgtt tactaataaa tctcctgaat
     9901 tataggtata gtaccattta ccagaaactg taacccaacc agtagccatt gaacctgatt
     9961 catttaagta ataccataag cctttgtctt taacccaacc agtagccatt gaacctgatt
    10021 catttaagta
//
LOCUS       DAVBCG010000071         9760 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBCG010000071 DAVBCG010000000
VERSION     DAVBCG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.071"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            271..366
                     /locus_tag="VQ495_001393"
                     /pseudo
     CDS             271..366
                     /locus_tag="VQ495_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(619..1239)
                     /locus_tag="VQ495_001394"
     CDS             complement(619..1239)
                     /locus_tag="VQ495_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2531344.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1239..1721)
                     /locus_tag="VQ495_001395"
     CDS             complement(1239..1721)
                     /locus_tag="VQ495_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2531345.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1721..2563)
                     /locus_tag="VQ495_001396"
     CDS             complement(1721..2563)
                     /locus_tag="VQ495_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2531346.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2658..3458)
                     /locus_tag="VQ495_001397"
     CDS             complement(2658..3458)
                     /locus_tag="VQ495_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2531347.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3602..4981
                     /gene="glmU"
                     /locus_tag="VQ495_001398"
     CDS             3602..4981
                     /gene="glmU"
                     /locus_tag="VQ495_001398"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2531348.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4991..5536
                     /locus_tag="VQ495_001399"
     CDS             4991..5536
                     /locus_tag="VQ495_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2531349.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5548..5862
                     /locus_tag="VQ495_001400"
     CDS             5548..5862
                     /locus_tag="VQ495_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2531350.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5879..6571
                     /locus_tag="VQ495_001401"
     CDS             5879..6571
                     /locus_tag="VQ495_001401"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2531351.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6648..7139
                     /gene="rocS"
                     /locus_tag="VQ495_001402"
     CDS             6648..7139
                     /gene="rocS"
                     /locus_tag="VQ495_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2531352.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7237..7827
                     /locus_tag="VQ495_001403"
     CDS             7237..7827
                     /locus_tag="VQ495_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2531353.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            7836..9599
                     /locus_tag="VQ495_001404"
     CDS             7836..9599
                     /locus_tag="VQ495_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2531354.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3004 a         1699 c         2084 g         2973 t
ORIGIN      
        1 caccccaaaa gttagatttt ttctgtctaa cttttggggt gtagttcatg tacacctgat
       61 atgatgcgtt ttataatttt aaagactttt tgaccagcct cattttttta acttgatact
      121 cagtgaaaag caaagattaa actaggaagc tagctgtagg ctgctcaaag aacagctttg
      181 aggttgcaga taaaacttgt gaggtcacca acatatataa tgtgaagctg acgtggcttg
      241 aatagatttt agaagggtat gagtctggaa gttttaatgg ataatgcaag attccataga
      301 atgggtaagc tagagttctt atgtgaagag tttgggcata aacttttacc ttttcctccc
      361 tactcatctt agtatagaaa agtgaatcta aaatagtaca taactgcttc taaaacattc
      421 ttataaattg atttaaattc tcaaatcata ttattcagtt cttatttcat tttgctctac
      481 aatcctgttg agaagacacg tgttcatatc aaaaaggtat tggcaagttg caataccttt
      541 ttacaaggtt cttttgtctt atttttgttt caactgacta tatctcctat ggttctagtt
      601 cagaaggcta ggctataatt atgattgata agaagtatca ttccaagtat tgagagtgaa
      661 tgtttcaaaa tcatgggttt ctataatggt caggctggca tttgctagac cgccatcttt
      721 acgaagaagt ggttctttat agcctaggag agtacgaaga ctggcagtaa gattggcgcc
      781 gtgtccgaca attagaatac gctcagctgg actatctttt aatgatttga taaattggat
      841 ggtccgttga gttgtactat agagggattc ggctccgaac attcgagtgt caaattgagc
      901 aagatttgaa cgaaaagcct ggatttgttg cgggtaaata gcttccaagg ttgcaatttt
      961 caaaccttct aacttcccaa gttgccattc acggagatta ggaacgattt ctaaagaaca
     1021 gggggtatag agttgacttt ggataatctc agcagatttg accgctcgag gtaaatcact
     1081 tgaataaatc tgatcaaaag gaatttcctt gagatactgg ccaagtcgtt ttagggtttc
     1141 aatggattca ggaagaagag gagaatcacc gctagcacct tgaaaacgac cttcttggtt
     1201 ccagagggta cgaccgtggc ggacaaagta gagtttcatt acttgatgtc ctccaaaata
     1261 tctacaaagt ctgcctttac aaagctagcc aagtcttgtg gcgcgacgat aatgctgtgt
     1321 ccgacttcgc ctgcagagac aatcattcga tccaaatcta gagcaatttt atcgataaaa
     1381 atgggataat tgtgtttctg acgaattccg acaggattat tggctccatg aatgtaacca
     1441 gttgtttttt ctaagtcctt ttgtggaatc atgctcactt ttttattgcc agaaatttta
     1501 gctagtttct tttcagacaa gtgctgagtg atagggacaa ttccgataat cggtccggtc
     1561 ttgtctccca aaagcgccaa ggttttgaaa atctgatctc gttcataacc ttgaggaagc
     1621 tctccttcta gggcattgat ttgaatcccc tgatgaggga tagctgcttt agataggatt
     1681 tgttccacca atgttttttt gattttaact ttttttgcca ttatttatat ttatcctcca
     1741 attgactcat ccaaatacca agccagattc ccagcgcaaa gaagaaggcg atgatgacat
     1801 aaccgacaag tgaaagtcct gtgtattgga tactttcagc gtttcctgca tttggaatta
     1861 agatcaaaag ggtacttgat aggacgatac cgatgatgaa atgatagacg cgtgagtggt
     1921 agttgtttaa ggcataatcc atcaattttg aaaaaacgat gagagttgca cctgcaccaa
     1981 ttccaatcgg aaagaaggtt cccaagagat caaaagtttt aaaaccagtc aacataggag
     2041 catagagtcc caaaatcaaa agtaagttcg atgggctgag gccaggaacc aagacgccaa
     2101 gggccaatag tgcgcctgct aggacgaagt taagaaagct ggcgcttaag gttccaacga
     2161 caaaatttaa ggcatagagt cctaatccag aaatgataaa ggttgtccat aaccaagcta
     2221 aatcaatctt gtctcggtca gattctcgag ttgattcttt gaggaggcta ggaactgtac
     2281 cgataatagc tcccgcaaag ctccataata caaagacctg ataattttca agcaggtatt
     2341 caatcgggta ggaaaataag ccgattccca gaagcatacc gatggcaact ggaataaagt
     2401 acaaaacatt ttctttaagg tctttaaagg gatgggccag aaagccaatc attcgttcat
     2461 agattcctaa gattgctgct agaacccctc cggaaattcc cggtaggata aatccaagag
     2521 caattacaat ccctttaata acgcgtgcta accatgagag catatatttc ctccaactat
     2581 ttctatttca aaaattcctt actatattgt atcacaatca gacacaaaaa gaaaaagcaa
     2641 atgataaaca aatgctttta aggttttaaa aaaagttttc gaaaggtttc ttctttattt
     2701 tttaagggag agataacgtt gatatctaaa tcgtggtcaa agccggcaat ttttccttta
     2761 gatgtgtatt ggtgaatatc ataatctaaa tcagttttag gactgctctc caaaaatcct
     2821 gagtctgagc cgtaggacgg aatccaaaca gaggtaaact tgcctgtatc aatactgtgt
     2881 tcttccatga agtagacacc aacgtagatg ccgatgtttt tagcacctag tgatgctagt
     2941 tttgctcgaa agttttcgac accttcgttc atattagaca tggttttgtc ttccacgtca
     3001 agccaatagt aactagggct gtaaggagag gcagcattgt agaaaacttc ggcagccttt
     3061 tccatttctt ggacactttt tccagctaca taagcgtaga cagcaactgg gacattccgc
     3121 ttttgaagtt cagtgatatg actcttatag gccttgtcta ttccattgat aaatgaagca
     3181 tcattttctt ttgttgtttg agcaccactg tgaacacgaa caatagcacc tgaaatattt
     3241 tgtgagaggg catcgtagtt gatttcctca ggacgctgcc agccagagag gtcaataatc
     3301 ggtttgtcta agtgtttcaa agcctgtgct tcaatctgtg ctatattgga ttttgtttta
     3361 aacgattggc tgtcattaag tgggcgattg atgattaaaa tgaacatcat aatcccaaaa
     3421 aaactaaata aaataagtgg atgaatttgt tttctcatat cttataagtc taccctaaaa
     3481 atcaaaaaaa atcaaaaaaa tgggttaagg aagagacttt agagcatttt ttcattcaag
     3541 agtgcggaat gatttgaaat atggtataat aaaagggaat ttctacagaa aagagaagat
     3601 tatgtcaaat tttgccatta ttttagcagc gggtaaaggg actcgcatga aatctgattt
     3661 gccaaaagtt ttgcacaagg ttgcgggtat ttctatgttg gaacatgttt tccgtagtgt
     3721 gggagctatc caacctgaaa agacagtaac agttgtagga cacaaggcag aattggttga
     3781 ggaggtcttg gctggacaga cagaatttgt gactcaatct gaacagttgg gaactggtca
     3841 tgcagttatg atgacagaac ctatcttaga aggtttgtca ggacacacct tggtcattgc
     3901 aggagatact cctttaatca ctggtgaaag cttgaaaaac ttgattgatt tccatatcaa
     3961 tcataaaaat gtggccacta tcttgactgc tgaaacggat aatccttttg gctatggacg
     4021 aattgttcgt aatgacaatg ctgaggttct tcgtattgtt gagcagaagg atgctacaga
     4081 ttttgaaaag caaatcaagg aaatcaacac tggaacatac gtctttgaca acgagcgttt
     4141 gtttgaggct ttgaaaaata tcaataccaa taacgctcaa ggcgaatact atattacaga
     4201 cgtcattggt attttccgtg aaactggtga aaaagttggc gcttatactt tgaaagattt
     4261 tgatgaaagt cttggggtaa atgaccgtgt ggcgcttgcg acagctgagt cagttatgcg
     4321 tcgtcgcatc aatcataaac acatggtcaa cggtgttagc tttgtcaatc cagaagcaac
     4381 ttatatcgat attgatgttg agattgctcc ggaagttcaa atcgaagcca atgttacctt
     4441 gaaagggcaa acgaaaattg gtgctgagac tgttttgaca aacggtactt atgtagtgga
     4501 cagcactatc ggagcaggag cggtcattac caattctatg attgaggaaa gtagtgttgc
     4561 agacggtgtg acagtcggtc cttatgctca cattcgtcca aattcaagtc tgggtgccca
     4621 agttcatatt ggtaactttg ttgaggtgaa aggatcttca atcggtgaga ataccaaggc
     4681 tggtcatttg acttatatcg gaaactgtga agtgggaagc aacgttaatt tcggtgctgg
     4741 aactattaca gtcaactatg acggcaaaaa caaatacaag acagtcattg gagacaatgt
     4801 ctttgttggt tcaaattcaa ccattattgc accagtagaa cttggtgaca attccctcgt
     4861 tggtgctggt tcaactatta ctaaagacgt gccagcagat gctattgcta ttggtcgcgg
     4921 tcgtcagatc aataaagacg aatatgcaac acgtcttcct catcatccta agaaccagta
     4981 ggagcctatc atggagtttg aagaaaaaac gcttagccga aaagaaatct atcaaggacc
     5041 aatatttaaa ctggtccaag atcaggttga attaccagaa ggcaagggaa ctgcccgacg
     5101 ggatttgatt ttccacaatg gggctgtctg tgttttagca gtaacggatg aacaaaaact
     5161 tatcttggtc aagcagtacc gcaaagctat cgaggctgtc tcttacgaaa ttccagccgg
     5221 aaaattggaa gtaggagaaa acacagcccc tgtggcagct gcccttcgtg aattagagga
     5281 agaaacagcc tatacaggga aattagaact cttgtacgat ttttattcag ctattggctt
     5341 ttgtaatgag aagttaaaac tatatttagc aagcgatttg acaaaagtgg aaaatccgcg
     5401 tccgcaggat gaggatgaaa ccttggaagt ccttgaagtg agcttagaag aagcgaaaga
     5461 attaatccaa tcaggtcata tctgtgatgc caagacaatt atggctgttc agtattggga
     5521 gttgcagaaa aaatagagga ggtcagtatg ggtaaatctt tattaacgga tgaaatgatt
     5581 gaaagagcta atagaggcga aaaaatttca ggtcctcctt tgctagatga taatgaggaa
     5641 actaagattt taccaacctc ttcttcccgt tttggttatg ccaatcctaa ggatcatggt
     5701 tttagccagg aaaccttgaa gattcaggtc gaaccatcta ttcataaaag ccgtcgtatt
     5761 gaaaatacca agagaaatgt cttcaattct aagttgaata aaatcttatt tgcggtcatc
     5821 tttctcttga ttttgcttgt tttagcaatg aaacttttgt aatagaaaag gaattgaaat
     5881 gaaaatagga attattgctg ctatgccaga agaactggct tatctggtcc agcatttaga
     5941 taatacccag gagcaagttg ttttggggaa tacctatcat acaggaacca ttgcttctca
     6001 tgaagtcgtt cttgtagaaa gtggaattgg taaggtcatg tctgctatga gtgtggcgat
     6061 tttggctgat catttccagg tggatgccct tattaatacg ggttcagctg gggcagtagc
     6121 agaaggtatc gctgttgggg atgtcgtgat tgctgacaaa ttagcctatc atgacgtgga
     6181 tgtcacagct tttggctatg cttatggaca aatggcgcaa caaccgcttt atttcgaatc
     6241 agacaaaacc tttgttgctc aaatccaaga gagtttatct caattggacc aaaactggca
     6301 tcttggtttg attgctacag gagatagttt tgttgcagga aatgacaaga tagaagcgat
     6361 taagtcccat ttcccagaag ttttagccgt ggagatggag ggggcagcta ttgctcaagc
     6421 agcgcatgcc ctcaatctcc cagtcttagt catcagagct atgagtgaca atgccaacca
     6481 tgaagcaaac atcttttttg atgagtttat tatcgaagct ggacgtcgct ctgcccaagt
     6541 cttgttgacc tttttgaagg ctttagatta agcggaaatt tgacagtttt tctagcttat
     6601 gataagattt aagtaaagaa aagctagaaa acgtttcaga ggatattatg agtattgaaa
     6661 tgaccgtcag tgagattgca gaggtcttag gattatctcg ccaagcaatc aataaccgtg
     6721 tcaaagaatt accagaagaa gacacagata aaaatgacaa aggggtaaca gttgttacca
     6781 gaagtggctt gattaagcta gaagaaatct ataaaaaaac gatttttgaa gatgagcctg
     6841 tcagtgaaga tgtcaaacaa cgtgaactga tggagattct agtggatgag aagaatgcag
     6901 aaattcttcg tctttatgaa caattaaaag ccaaggatcg tcagttatca gaaaaagacg
     6961 agcagatgcg tatcaaagac cgtcagattg ctgagaaaga caaacaatta gatcagcaac
     7021 aacaattgac ccttcaagcc atgaaggatc aagaaaatct taagctagag ctggaccaag
     7081 caaaagaaga agtccaatcc actaagaaag gcttttttgc tcgtttattt ggaggataat
     7141 caaggagctg tttaggttaa atgctaacct gatggcttct tttgtttgtg aatagtatag
     7201 ttgattagaa cagagacagt agaggagagg tacagaatgg aacgattaga agattacatt
     7261 gcttttgact tagaatttaa tcagcacgaa ggacaaacac acttgattca agtatcggca
     7321 gtgcgtttta gagatggtcg ggaaatcgat gcctatgatt cctatgttca taccagcgtc
     7381 cctctaaaaa gctttatcaa tggtttgacc gggattacag ctgagacctt aaaagatgct
     7441 cctccagttg agaaagtctt agaagaattc caagagtttg tgggttcttt gctgatgata
     7501 ggctacaatg ctgctaaaag tgatttacct attttagcag agcatggttt agactatagc
     7561 caacagtaca aggtggattt gtatgatgaa gcttttgaac gtcggagttc agatttacat
     7621 ggtattgcca atctcaaatt gcaaactgtt gcctcatttc ttggatttca aggtcagtct
     7681 cataatagtt tagaggatgc tcggatgacg gcgcgtgttt acgaatcctt cttagagtcg
     7741 gatcaagcta gggaattgtt agagacagaa agtagcttat caaacaatcc ttttggtgtc
     7801 ttggacttgt ctcaattatt tgactaggag tgcctatgaa acctgaaaaa cctaaaaaat
     7861 tgggtcttaa acagatttac ctcaatcttg ataaaatcct ctttctattt ttcttgattt
     7921 ttatgatagt ggagtatttg tgggtaccat tcaattcatg gttggctagc ttcttattag
     7981 atcagacagg ttatcttttt atttcctata acaatgtctt agccatcctt actcattctc
     8041 cattgattag tctagctttt ttagccttga ttgtaattaa ccttctggtt gcttattttc
     8101 aaataggact cctctttatt ggtgctcgtc atctcctcta taatgaaaag cgaaggctaa
     8161 ttgagtatat ccgcaaggtt ttccatgaaa gttttggatt tatgaaaaag ctaacttgcg
     8221 ctaaagcctt gtttatcttt ttctatatag ctatgctttt tccttttata cggaaaatgc
     8281 tgaagattta ttatcttaat aaaatcgtca ttccagagtt tatccaagct tatttagaag
     8341 ataaatattg gatgtggtgg ctgagcatcc tacttctatc tcttatcttt ctctatgtct
     8401 ctgtcagatt gatgtttgcc cttccgaaga ttgtatatga tcagctgact gttcgagaag
     8461 cagtgatgtt tagcttggag aaaaccaaga agagagttac tttttatgct tggaatttat
     8521 tttacatctt gctcaaagca catttattat tttatcttcc tttgattcct ttattattgg
     8581 ctcaaacttt ggtagatgat atcactcaga gagagtctct gattcttggt attttgaatt
     8641 ttgtcgtgat taaaaatctc tattatatgg ctctgactta ttttttggtt aaatttgttt
     8701 catttttgac aggaaaggag ctggatatgc ttcctaggag agaaaaagat cacatcgtgc
     8761 gatggggtgt catgacttgt gccagtcttt tctttgcctt agaaggttat atttatctgg
     8821 aggctcccat ggttcatcta cctcaactta tttctcaccg aggggtttcc aatgcaaatg
     8881 ggattcaaaa tacagtagag tccttggaaa ctacagcaca actcaaacca gacttggtgg
     8941 agacggacgt gcaggaaaca aaagatgggc agtttgtcat gatgcatgat gctaacttga
     9001 aaaatctagc aggtatcaat aaaagtcctc aagacttaaa cttggaggag cttaaaggga
     9061 ttgatatttt tgaaaatggc taccagacta aaatttcaag ctttgaagat tatctcagtc
     9121 gagccaacga acttggtcaa aaattactaa ttgaaattaa aaccagtaaa aaagatagtc
     9181 cagacatgat gaaccgcttt ttagcccgtt atgctgcaaa gctcaagatt tatggacatc
     9241 aaatccagtc tttagactac catgttgtcg aaaaagtaag acagtatgat gccgaactgc
     9301 cagtttattt catcatgccc tacaattctg tctttcctaa aacaagagcg acaggatata
     9361 ctatggagta ctcaaccttg gatgaatatt ttgtaagcaa actatggaca acggatcaga
     9421 aactttatgt ctggactgtc aatgattcag aagctatcag caaatctctt cacttaggag
     9481 tagatggagt gattacagat gatttggaaa aagttcagcg ggaaatagaa gtagcccaag
     9541 aagatccaga atatacggat ttgctcttga aaaaagcatt ggaattcttt gaattttagt
     9601 aataaaaaag aggaacggtt gttcctccca tgataggtaa gtattcttgt gaatgcttgc
     9661 ttttttgtgg ttgcaatccg tttacgagaa ttgtttgtat tgttcttgtt tcaatttact
     9721 ttatagtgct ttgaagttgg aatgctacac tataacttct
//
LOCUS       DAVBCG010000072         9726 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBCG010000072 DAVBCG010000000
VERSION     DAVBCG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.072"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..150
                     /locus_tag="VQ495_001405"
     CDS             <1..150
                     /locus_tag="VQ495_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531355.1"
                     /translation="YYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNAN
                     GEWVN"
     gene            complement(380..1360)
                     /gene="dusB"
                     /locus_tag="VQ495_001406"
     CDS             complement(380..1360)
                     /gene="dusB"
                     /locus_tag="VQ495_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2531356.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1347..2219)
                     /gene="hslO"
                     /locus_tag="VQ495_001407"
     CDS             complement(1347..2219)
                     /gene="hslO"
                     /locus_tag="VQ495_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2531357.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2384..3811
                     /locus_tag="VQ495_001408"
     CDS             2384..3811
                     /locus_tag="VQ495_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2531358.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3970..5478
                     /gene="glpK"
                     /locus_tag="VQ495_001409"
     CDS             3970..5478
                     /gene="glpK"
                     /locus_tag="VQ495_001409"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2531359.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5520..7346
                     /gene="glpO"
                     /locus_tag="VQ495_001410"
     CDS             5520..7346
                     /gene="glpO"
                     /locus_tag="VQ495_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2531360.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7416..8120
                     /locus_tag="VQ495_001411"
     CDS             7416..8120
                     /locus_tag="VQ495_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2531361.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8417..8620
                     /locus_tag="VQ495_001412"
     CDS             8417..8620
                     /locus_tag="VQ495_001412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531362.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8707..9295)
                     /locus_tag="VQ495_001413"
                     /pseudo
     CDS             complement(8707..9295)
                     /locus_tag="VQ495_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2818 a         1935 c         2198 g         2775 t
ORIGIN      
        1 tactatcttg aagcatcagg tgctatgaaa gcaagccaat ggttcaaagt atcagataaa
       61 tggtactatg tcaatggctc aggtgccctt gcagtcaaca caactgtaga tagctataga
      121 gtcaatgcca atggtgaatg ggtaaactaa acctaatata actagttaat actgacttcc
      181 tgtaagaact ctttaaagta ttccctacaa ataccatatc ctttcagtag ataatatacc
      241 cttgtaggaa gtttagataa aaaaataact ctgtaatctc tagccggatt tatagcgcta
      301 gagactacgg agtttttttg atgaggaaag aatggcggca ttcaagaggc tctttaagag
      361 agttacgggt tttaaactat taagccttct ccaattgcaa gagggcttca atctctgcca
      421 gggtgctagc ttgcgaaatg gctccacgga gtttggcagc gccagatgtt ccacggagat
      481 agtgaggagc gaggccgcgg aattcacgaa ctgcgacgtt ttctcctttg aggttaatca
      541 atcgtttcaa gtgttcgtag gcgatcttca tcttgtcttc aaaggtcaaa tcaggtagga
      601 tttctcctgt ttcaaagtaa tggttgattt ggttgaagag gtaaggattt cccatggcag
      661 ctcggccaat catgactgcg tcagcaccaa cttcttcgat gcgttgcttg gcttcttgga
      721 cagtacggat atcaccgttg gcgatgaatg gaatcttggt tagagcttgg gcaaccttgt
      781 aaagggtctc aaggtctgcg tggccagtat acatttgttc acgggtacgg ccatgcatgg
      841 cgagggcaga aacacctgca gcctcagcag cgagggcatt ttctactgca agagatgggt
      901 ccgcccatcc ggtacgcatt ttgacagtaa gtgggatatc aaggacagac tggaccttgt
      961 tgatgataga gtagatcttg tcaggatcct tgagccacat agctccagct tcgttcttca
     1021 cgattttgtt gacagggcag cccatgttga tatcgacgat atcggtcttg gtgttttctt
     1081 ggatgaattc tgctgcgcgt gctaggctgt cttcatcgct accaaaaagt tggatagaga
     1141 cagggttttc gccctcatcg atatgaagca tgtgcagggt tttttcgttg ttgtattgga
     1201 ttcccttgtc agagaccatt tccattacaa cgagtccagc tccgagctct tttgcgatgg
     1261 tacgaaaggc tgagttggtc acgccagcca taggcgctaa aacggtacga ttgggaatct
     1321 caatattgcc aatcataaaa ggtgtattaa gatttgtcac gaatgagttc ctccaggtcc
     1381 ttttcatcaa agttgtaagt agtttggcag aattgacaag tgatttctgc cccgtggtct
     1441 tcctctttca tttcctgtaa gtctgagctt ggaaggctgg caagagcgtt cataaagcgt
     1501 tcatggctac agtcacattg gaaacggatt tcttcttcag aaagacgctt gtaggcttcg
     1561 tccccgtaga tagccttgag gagggcttcg atatggtcgt cgctttcgag aagagtagag
     1621 atagctggca tttcttggat gcgtttttca aagcgagcaa tctcttcttt cttggctcct
     1681 ggcaagactt gaactaggaa accacctgca accttgacct tgtcttcctc gtccaaaagg
     1741 acattgaggc cgaccgctga aggcgtttgt tggctttcag taaggtaaaa ggcaaggtct
     1801 tcaccgattt ctccagagat gaggggagtt atagagttgt aaggatttcc agcaccgtag
     1861 tctgtgataa cgaggaattg accatttcca acaaaaggtc cgactaggac ttcaccagtc
     1921 gcagtctttt tgatgtcaac accaggattt tgaacatagc ctttgacgtt ccccttggta
     1981 tcagcgacgg tgataatagc acctagagag ctagagccca acaccttaac tgtaagtttg
     2041 gtatttcctt tttcattggc tgcgagaatc tggctagcga taagagttcg accaagcgct
     2101 acagttgagc tagcttgggt ttgatgtctt tcttgagcag tgcggacggt ttccgtgcta
     2161 tcaaggacaa aagcacgaaa ggctccgctt tctgatatag ttttaataat tttatccata
     2221 gctactattt tagcataaaa atgcccaaag ggggagccgt gtgtttactg attttcagga
     2281 taatggacca ggaaatcagc atgaaaataa aaagagaaac agattatttc agcatttgta
     2341 agatttatgc tatgcttaag gtagaaaatg aaaggggtaa caaatgtatt taggagattt
     2401 gatggagaaa gccgagtgtg gtcaattttc aatcctttcc tttctattac aagagtctca
     2461 gacgaccgtc aaggctgtaa tggaagaaac aggattttca aaagcaaccc taaccaaata
     2521 tgtcaccctg ctcaatgaca aggctttgga tagtggctta gaactgacta ttcactcaga
     2581 agatgaaaat ctgcgtctgt ctatcggtgc agctaccaag gggagagata ttcggagctt
     2641 gtttttggat agtgctgtta aataccagat tttggtttat cttctctacc accaacagtt
     2701 tttagcccat cagctggctc aagaattggt gattagcgag gctacgcttg gtcgtcactt
     2761 ggctggttta aatcagattt tgtcagaatt tgatttatcc atccaaaatg gacgctggag
     2821 aggtccagag catcagattc gctatttcta tttctgtctt ttccgaaagg tctggtcgag
     2881 tcaggaatgg gaaggtcaca tgcagaaacc agagagaaaa caggagattg ccaatttaga
     2941 ggaaatctgc ggtgcaagtt tgtctgcggg gcagaaattg gacttggttc tctgggctca
     3001 catcagtcaa caacgtcttc gggtcaatgc ttgtcagttt caagtcatag aagagaaaat
     3061 gcgagggtat tttgacaata tcttttatct tcgtttgctg agaaaggttc cgtccttttt
     3121 tgctgggcaa catattccac taggagttga ggatggtgag atgatgatat tcttctcttt
     3181 tctcctatct catcgcattc ttcctcttca tactatggag tatattcttg gttttggagg
     3241 gcagttggca gatttactga cgcaattgat tcaagaaatg aagaaggagg aactattggg
     3301 ggattataca gaggaccatg tcacctatga actcagtcag ctttgtgctc aagtctatct
     3361 ctataagggc tatattttac aggatcgcta caagtaccag ttagagaatc gtcatccata
     3421 tttactgatg gaacatgatt ttaaagagac agcagaggag atttttcatg ctctacctgc
     3481 ttttcaacag gggacagatt tagataagaa gattctctgg gaatggctcc agttaatcga
     3541 atatatggct gaaaacggtg gccagcatat gcggattggt ctggatttga catctggttt
     3601 tcttgtcttt tcaaggatgg cagccatttt gaaacggtat ttggaataca atcgttttat
     3661 taccattgaa gcttatgacc ctagtcggca ttatgatttg ctggttacca ataacccgat
     3721 tcataagaag gaacagacac cagtctatta tttaaaaaac gacttggata tggaagattt
     3781 ggcagggatt cgtcagttat tattcactta aaaggcttgg ttaatctagg tcttttttgt
     3841 gaaattcaca caatctcctc acattttttt aaaaattaaa aaaagttgat aaacaagaaa
     3901 gcgctttatt ttgtatacta gtaagtgtaa agaggaaaca cctcaagatc tttatcagga
     3961 ggacagtaca tgtcacaaga aaaatacatc atggccattg accagggaac tacaagttct
     4021 cgtgccatca ttttcaacaa aaaaggggaa aaggttagct cgagtcaaaa agagtttacc
     4081 cagattttcc ctcaggcagg ttgggttgag cacaatgcca atgaaatttg gaactctgtt
     4141 cagtcagtta ttgcgggtgc tttcatcgaa agtggtgtca agccaaatca aatcgaggca
     4201 atcgggatta ccaaccaacg tgaaacaacg gttgtctggg ataagaaaac aggacttcct
     4261 atctacaatg ctatcgtttg gcagtcacgc cagacagcac ctttggctga gcaactaaaa
     4321 agccaaggtt atgtggaaaa attccatgaa aagactggtt tgattattga tgcttacttc
     4381 tctgctacca aggttcgttg gattttggat catgtagaag gtgctcaaga gcgagcagaa
     4441 aaaggggaat tgctctttgg tactatcgat acttggttgg tttggaaatt gactgacggt
     4501 gcggctcacg tgactgacta ctcaaatgca gctcgtacca tgctttataa cattaaagaa
     4561 ctcaaatggg atgatgagat tttggaaatc cttaacattc cgaaggctat acttccagaa
     4621 gttcgttcta actccgaaat ctacggcaag acagctccat tccatttcta cggtggagag
     4681 gtgccaatct caggtatggc tggggaccaa caagcagccc tctttggaca gttggctttt
     4741 gagccaggta tggttaagaa tacttatgga acaggctctt tcatcatcat gaatactggg
     4801 gaagagatgc agttgtctga aaacaacctc ttgacaacca ttggttacgg aatcaacggt
     4861 aaggtttatt atgccttgga aggttctatc ttcatcgcag gaagtgctat tcagtggctt
     4921 cgtgacggtc ttcgcatggt tgaaaattca ccagaatctg aaaaatacgc tcgtgattct
     4981 cacaacaacg atgaagttta tgtcgttcca gcctttacag gtctaggcgc tccatactgg
     5041 aaccaaaatg ctcgtggttc cgtctttggt ttgactcgtg gaacaagcaa agaagacttt
     5101 atcaaggcga ctttgcaatc tattgcttat caagtgcgtg atatcatcga caccatgcaa
     5161 gtggatactc agaccgccat tcaagtactg aaggtggatg gtggtgcagc catgaacaac
     5221 ttcctcatgc agttccaggc ggatatttta ggcattgaca ttgcacgtgc taaaaacctg
     5281 gaaacaacag ctctaggagc ggccttccta gcaggtttgt cagtagggta ctggaaagac
     5341 ttggacgagt tgaaactctt gaacgagaca ggagaactct ttgagccatc tatgaacgaa
     5401 tctcgcaagg aacaactcta caagggctgg aagaaggctg tgaaagcaac tcaagtcttt
     5461 gcggaagtag acgactaata ctggcagaat aaagcgattt atttagaaag tgtgtaaata
     5521 tggaattttc aaagaaaaca cgtgaattgt caattaaaaa aatgcaggaa cgcaccctgg
     5581 acctcttgat tatcggtgga ggaatcacag gagctggtgt agccttgcag gcggcagcta
     5641 gcggtcttga gactggtttg attgaaatgc aagactttgc agaaggaaca tctagtcgtt
     5701 caacaaaatt ggttcacgga ggacttcgtt accttaaaca attcgacgta gaagtggtct
     5761 cagatacggt ttctgaacgt gcagtggttc aacaaatcgc tccacacatt ccaaaaccag
     5821 acccaatgct cttaccagtt tacgatgaag atggagcaac ctttagcctc ttccgtctta
     5881 aagtagccat ggacttgtac gacctcttgg caggtgttag caacacacca actgcgaaca
     5941 aggttttgag caaggatcaa gtcttggaac gccagccaaa cttgaagaag gaaggcttgg
     6001 taggaggtgg agtgtatctt gacttccgta acaacgatgc gcgtctcgtg attgaaaaca
     6061 tcaaacgtgc caaccaagac ggtgccctca ttgccaacca cgtgaaggca gaaggcttcc
     6121 tctttgacga aagtggcaag attacaggtg ttgtagctcg tgatctcttg acagaccaag
     6181 tatttgaaat caaggcccgt ctggttatta atacaacagg tccttggagt gataaagtac
     6241 gtaatttgtc taataaggga acgcaattct cacaaatgcg cccaactaag ggagttcact
     6301 tggtagtgga ttcaagcaaa atcaaggttt cacagccagt ttactttgac acaggtttgg
     6361 gtgacggtcg tatggtcttt gttctcccac gtgaaaacaa gacttacttt ggtacaactg
     6421 atacagacta cacaggtgat ttggagcatc caaaagtaac tcaagaagat gtagattatc
     6481 tacttggcat tgtcaacaac cgctttccag aatccaacat caccattgat gatatcgaaa
     6541 gcagctgggc aggtcttcgt ccattgattg cagggaacag tgcctctgac tataatggtg
     6601 gaaataacgg taccatcagt gatgaaagct ttgacaactt gattgcgact gttgaatctt
     6661 atctctccaa agaaaaaaca cgtgaagatg ttgagtctgc tgtcagcaag cttgaaagta
     6721 gcacatctga gaaacatttg gatccatctg cagtttctcg tgggtctagc ttggaccgtg
     6781 atgacaatgg tcttttgact cttgctggcg gtaaaatcac agactaccgt aagatggctg
     6841 aaggagctat ggagcgcgtg gttgacatcc tcaaagcaga atttgaccgt agctttaaat
     6901 tgatcaattc taaaacttac cctgtttcag gtggagaatt gaacccagca aatgtggatt
     6961 cagaaatcga agcctttgcg caacttggag tatcacgtgg tttggatagc aaggaagctc
     7021 actatctggc aaatctttac ggttcaaatg caccgaaagt ctttgcactt gctcacagct
     7081 tggaacaagc gccaggactc agcttggcag atactttgtc ccttcactat gcaatgcgca
     7141 acgagttggc tcttagccca gttgacttcc ttcttcgtcg taccaaccat atgctcttta
     7201 tgcgtgatag cttggatagc atcgttgagc caattttgga tgaaatggga cgattctatg
     7261 actggacaga agaagaaaaa gcaacttacc gtgctgatgt cgaagcagct ctcgctaaca
     7321 acgatttagc agaattaaaa aattaagaaa aaataaaaga ggtggagggc agcattcctt
     7381 gtcgcccgtc ccttcttttt aatggagaca gaaagatgat gaatgaatta tttggagaat
     7441 ttctagggac tttaatcctg attcttctag gaaatggtgt tgttgcaggt gtggttcttc
     7501 ctaaaaccaa gagcaatagc tcaggttgga ttgtgattac tatgggttgg gggattgcag
     7561 ttgcggttgc agtctttgta tctggcaagc tcagtccagc tcatttaaac ccagctgtga
     7621 ccatcggtgt ggccttaaaa ggtggtttgc cttgggcttc cgttttgcct tatatcttag
     7681 cccagttcgc aggggccatg ctgggtcaga ttttggtttg gttgcaattc aaacctcact
     7741 atgaggcaga agaaaatgca ggcaatatcc tggcaacctt cagtactgga ccagccatca
     7801 aggatactgt atcaaacttg attagcgaaa tccttggaac ctttgttttg gtgttgacaa
     7861 tctttgcttt gggtctttac gattttcagg caggtatcgg aacctttgca gtgggaactt
     7921 tgattgtcgg tatcggtcta tcactaggtg ggacaacagg ttatgccttg aacccagctc
     7981 gtgaccttgg acctcgtatc atgcacagca tcttgccaat tccaaacaag ggagacggag
     8041 actggtctta cgcttggatt cctgttgtag gccctgttat cggagcagcc ttggcagtgc
     8101 ttgtattctc acttttctag tttatactct tcgaaaatca aattcaaacc acgtcagcgt
     8161 cgccttaccg tactcaagta cagcttacgg ctagcttcct agtttgctct ttgattttca
     8221 ttgagtatta gaaaacaatt atgttgatag agcttgggca agagcccaat ttcagcaaaa
     8281 aatgaagtaa atcttctcat aataaaacgc atcatatcaa gcacgaaaat tccacgaggt
     8341 caactacagt cagaaagctg aacaacaagc caaaacgccc aaaaaaggcg gcaaaaagca
     8401 agcacctgca agcaacgtgc cgaaatggtc aaatcctgat tatgtcaacg aattagaccc
     8461 aaaaatcgtt gatatgctag tagaatttca caagtcacaa ggcactttgg aaactcccga
     8521 ggcgcaagca gaaatcgccc aaaaacgtga agaaatcgag caaaggagag ctgagcttga
     8581 ggataaaaaa caagagcttt tgaaccgctt gaacaaatag agtttcgcaa gtattatgct
     8641 tacaaattac ttgagcaatt aactaaaata taaaccctgc ctttatatct aggcagggtt
     8701 tatattttag aaattcacgt aggttgttac ggtttttaca tacccagtat agtttgagtt
     8761 tctatagtat tcagtgataa acttccattt ttttttgagc aacatggata taagtacttg
     8821 ttatgtagta tggatatggg ctttgtgaat ccaagtaaga ctgataagct tgtataccaa
     8881 aatatgctcc accaattatt gcaccccatg gaccccccaa taaagtacct atcctaccaa
     8941 tcatataact gattccagca ccagtcatga agttagcgaa tgtgttagct tgtttattcc
     9001 catgtattgt gttgacgtaa ttccaaacat taggatcgta tgatctaaaa gatatattta
     9061 ggtcgatttc attcttttga taagccatat aaaatgcccc attgatatag acgccgtcag
     9121 cacgtcgttc aatagtgtct acacttccat ctggattgac aacctcaaga acttcatcgc
     9181 ttaaaatatt tacttgcgta tctccgaacc gcactgatga gccattctca aactgagcct
     9241 caccagatac aactttagag tttgccgata agctatcatc agcaaaaaca aacaagcgac
     9301 ggggaaatgc tagacataca gaaaacagac ataactagca aacacatgca tttaaacatc
     9361 ttagacataa cggaaactcc tttgtatttt tgattttttt caacttttat tatacaataa
     9421 aaccaaataa aaagaaagcg gtaacaatat gcttaatgcg aaaatttttt atatattttt
     9481 atgtttgatc gttatcgaaa ctacaggctt gttgttgttg aaaagaggtc tcgaaatggg
     9541 ttatttagac acagaagcta ttatcctcgc agttttttca tttgcttttt acaacctatg
     9601 ttcattcgct tgggtctgct ctacaataaa aaacaataaa aaataaatag acgtattttc
     9661 aaaaaaaaaa aaaaaaaatg catatttatc ttagcaaaac gacaatttaa atcgtcgttt
     9721 ttttgt
//
LOCUS       DAVBCG010000073         9594 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBCG010000073 DAVBCG010000000
VERSION     DAVBCG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.073"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..947)
                     /locus_tag="VQ495_001414"
     CDS             complement(<1..947)
                     /locus_tag="VQ495_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2531363.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
     gene            complement(1027..1890)
                     /locus_tag="VQ495_001415"
     CDS             complement(1027..1890)
                     /locus_tag="VQ495_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2531364.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(1893..3794)
                     /locus_tag="VQ495_001416"
     CDS             complement(1893..3794)
                     /locus_tag="VQ495_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2531365.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            complement(4222..4497)
                     /locus_tag="VQ495_001417"
     CDS             complement(4222..4497)
                     /locus_tag="VQ495_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2531366.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(4603..5442)
                     /locus_tag="VQ495_001418"
     CDS             complement(4603..5442)
                     /locus_tag="VQ495_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2531367.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            5584..6432
                     /locus_tag="VQ495_001419"
     CDS             5584..6432
                     /locus_tag="VQ495_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2531368.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(6525..7442)
                     /locus_tag="VQ495_001420"
     CDS             complement(6525..7442)
                     /locus_tag="VQ495_001420"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2531369.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            7879..8084
                     /locus_tag="VQ495_001421"
                     /pseudo
     CDS             7879..8084
                     /locus_tag="VQ495_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQ495_001422"
     CDS             complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQ495_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2531370.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(8705..9049)
                     /locus_tag="VQ495_001423"
     CDS             complement(8705..9049)
                     /locus_tag="VQ495_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2531371.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQ495_001424"
     CDS             complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQ495_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2531372.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(9415..9502)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
BASE COUNT         2931 a         2014 c         1613 g         3036 t
ORIGIN      
        1 aaaatcgtca taaacaattc acaaactaaa tttccagaaa aggaaagaaa agttggaagt
       61 ggtaatccca tcataaaaac tccgacacct gtcaaagcca gtaaaatcaa aagattataa
      121 atattagctt taattttact agctagaaga gccccaatga tggaaccaat agcccccata
      181 gttaaaatac ttgcataggc tccttctgac ccgtaaagct gattcgaaaa gggaagtaga
      241 aattcaaaag ctgcaaaaaa gaaattaacg ctggaagcta ccagcaaaag gaagaaaatt
      301 tcttgctgat gccagatata gtgtaaccca tccttgatat ctacaaaaat atctctccca
      361 gtaaaagcct ttttctcttg aacttttgct tcctcttttg gaaggaaagt cactagaata
      421 aaagcaatga aaaaagtcag cgagtctagc agtagcgtca tatggagact tgcaaactgt
      481 aaaacaagga aggaaagaac aggagagcta acacctacaa cctgcaaaac cagctctaag
      541 cgagaattat agatcacaat ctcatctttc tccaccactt cagttatgat agctttattg
      601 gctgtgcgag aaaaggcaaa agcaataacc tgcacaatgt tagcaacaat caaagcgcca
      661 atcatccagc tatcattcct tatgaaagaa atagccagac aaagaatccc acaaacaaga
      721 tctgccgtca ttaaaatctt acgacgagaa aaacggtctg aaataactcc gccaaaggga
      781 ttgacgagaa tagatgtgac gagctcagaa atctgataca ttcctaaaac tgtctgtcct
      841 atagtcccca tagaagccaa ccagacacta tttccataat catagagcat atttcccatt
      901 ttattgatag ccccacggct aatcaactgc actgcatagc gattcatatt aaagctcctc
      961 tcaaattttg aaactattgt atcaaaaccg aaaggagctt tttatttttt cccttatttg
     1021 ggaaaattaa cttttgacaa atttttcgta gtgttcctga taataggcta cttgctctgg
     1081 aagacctaac acatcaaaaa tatgcatggc ctcttgcatc tgcttacagc cttctttaca
     1141 ctgtcctttt tgatataagg caaaaccttt taaataatgg aaaacattac gctcataaag
     1201 cttaatacct ttgtcaataa tcttctctgt ataagcctca aaatagttgg cattataaaa
     1261 agaagaatgc tctaaacaat gctggtaaca attgagggcc aaaatcaaca ctaatctctt
     1321 atggcgacta atctcttggt aaaattcctc cctctccata acttctctac caatccgagt
     1381 gacatagtct acatcgtaga aactatagag gttaccgaaa agaatcaact catacatggt
     1441 ccattcttct gttttgaaga gataatctgc taccttatcc aaatcatcct gcttcatatc
     1501 ataactcgca tctctttgac aaatcagacc ttgtagcaaa atccagttca gctcaaaata
     1561 aagggaagtc gtcgaactct tagacttttc aagttgttct ctttgaagct tttgaaaacc
     1621 tgcaatatcg tttgaatagt aaagtgggat aatctgtgcc atcatagaca catgttcatg
     1681 attatgaaaa ttccttgcct tatccatgaa attttcgatt gttacatgaa tgttatccaa
     1741 aatctcaaag aaacgggaga ctgccaggtc agactcccca agctcaaagc gagataactg
     1801 agaggtagag caggattcgc ctgctgcttc ctttaaagaa taatttccac ttgttcgaaa
     1861 ttcacgaaat acttttccaa gatgttccat ctttacacct gatctgataa ttcttcccac
     1921 tcaagcatag cttcttcctg acgatggctg attttatcca gctcagcctg taattccatg
     1981 agtttgtcgg catcgtttgt ttccaacatt tgttcagaaa tggcttggct ttgactttct
     2041 agctcttcaa tttcagcttc tagactttcg atttgtcgca tgagtttgcg aacttctttt
     2101 tgactttctt tctgggcctg atagtcattg actggacttg cttcctttgc ttgattgcta
     2161 gttgaagctt cctcagtctg actcatttct gctgttgctt tcttctcaac atagtagtcg
     2221 taatctccaa ggtagagagt tgaaccattc tcagacaatt ccaaaacatg agttgccaca
     2281 caattgataa agtaacgatc atgactgaca aaaagcaagg taccatcaaa gtcaatcaag
     2341 gcattttcta gcacttcctt actatcaata tccaagtggt tggtcggctc atccagaatc
     2401 aaaaagttat tgttttccat agacaattta gctaaaagca aacgagcttt ttcgccacca
     2461 gatagcatgc cgactgattt tttaacatca tctcctgaga aaaggaaggc tccaagacgg
     2521 ttgcggattt caacttctgg tgtcaatttg aaatcattcc agagttcatc cagcaccgta
     2581 ttacttggtg tcagcttgct ttgggtttgg tcatagtaac caacctcaac attagcgcca
     2641 aagcgctttt ctcccttgat aaacggaatt tgatccacga ttgatttgat aaaggttgat
     2701 tttccgatac cattgggacc aacgatagca acagcattca tcttacgaag gtctaggtta
     2761 atcggttgtg acaggacttc cccatcatag ccaacagccg cattttcaac agtcaaaaca
     2821 acattgcccg acgttttttc agactggaag gtcatgttgg ctgctttctt gccagcttcc
     2881 ggcttgtcca aacgctccat tttttctagt tgtttacggc gagattgagc acgtttggtc
     2941 gttgaagcac gaactagatt gcgattgaca aagtcttcca gagcagcgat ttccttctgt
     3001 tgcttttcat agttttttgc ctcagtagct agcttttgct ccttcaactc gacaaaacga
     3061 gagtaattcc ccacatagcg atccaaggaa tgcttggtca aatctagcgt aattgtcgca
     3121 accttgtcca agaaataacg gtcgtggctg acgataatga gggcaccgct atagtttacc
     3181 aagtaattct ctagccagga gatggtttca atatccaagt ggttagttgg ctcgtccaag
     3241 accaagagat tgggcttttc aaggagcatt ttagctagag ccaaacgagt attttgacca
     3301 cctgaaagtt cagctatttt catctgccac atagactcgt caaacttgaa tccattcaaa
     3361 atcgctcgaa tatcagcttc ataggtaaag ccacctgctt ggcgaaaatt ctcagataag
     3421 cggtcataat ctgacatcag tttatccaaa tcctcaccag acttttcacc catctccagc
     3481 tccatctgac gcagttgtct ctccgtccga cgcaaatcat taaagacatg aagcatttca
     3541 tcgtagatgg tattttcaga ctcaaaacgg ctatcttggg ctaggtaaga cagagaaata
     3601 tcttttttct tattgatttc tccgctagtt ggctcctctt ctccaactaa aatcttcaaa
     3661 agagtagact tacctgcacc atttttccca acaagagcaa tccgatctcg ttcatcaacc
     3721 tgcaggttga tattatcgaa aagaacctct cctgcaaaag aacgttcaat tttattagct
     3781 tgtaaaataa tcataagagt agtatagcat gtttccctaa ggcattcaag ataatcgtaa
     3841 gtcttttagt acaactttta taacataaaa taaactaaat tatgtatatt ttatattaga
     3901 ttccttcact atcttgttgg attttctaac cagctaatct tgtttcaaat agttatcgca
     3961 caagcctatt atttaattct tttcattatt tacgtacgcg tatagcagat tgaaataaga
     4021 tgagaacaaa tcgattggga aagtaaaatt aatttctata aatgttttag caattgtttc
     4081 gtactatttt agattcagtc tactatgtac aatattttcg gaacattcaa ctttttaact
     4141 ctatttatta ctagatttca taattaaaaa gcctactgac caagctagaa agcttgatac
     4201 aataggcttt ttaaagactg attatttaac agcgtcttta agagctttac cagctttgaa
     4261 tgctggtact ttagaagctg caattgtcat ttctttacca gtttgtgggt tgcgaccttt
     4321 acgttctgca cgctcacgaa cttcaaagtt accaaaaccg atcaattgaa ctttttcacc
     4381 agctgcaaga tagtcagcta ctgctgcaaa tacagcttca actgctgctg ctgagtcttt
     4441 cttagtcaat tctgtagctt ctgctacttt agcgatcaaa tcttgtttgt ttgccatgtt
     4501 aatgattcct ccagataatt tctaattaac aaatataatc atatcctaaa atccctttta
     4561 ggtcaagtta aaaacgctat ttcttccaat tttatttatt ttttaggagt ggtaacgtat
     4621 caaaatagcc caagcgttct cacccgtgtg agtttgaata atggaacccg tttccaaaac
     4681 agaaattggc ttttcaacat aagcttgtaa gctttctttc atctcttttg cccaatcatc
     4741 actaccagaa tatgaaattc caatctctgc tacagcacgt tcagaaagcg atgttatcaa
     4801 ctcatctaac cattttttaa atgttttagt tccacgacct ttaaccattg gctgcaattc
     4861 atggtctttc atttgcatga caacacggat attgagaagg gagctcaaca agccagttac
     4921 acggccaatt cgtccacctt tgacaagatt ttccaaagtt gaaacaccaa tataaagctc
     4981 tgtatggttt ttaacctctt ctacatgaga taaaattgcc tccatatctt taccttcttg
     5041 agctaacttc gcagcctcaa caacttggaa tttcagggct tggtcagtga aggaactatc
     5101 aacaacaatc acatctgcag tagataggct agcaccttgg cgtgctgctt ctaccgtacc
     5161 cgaaagagca tgggacatat gaatagcaag aatctggcca ccatctttgc ataggtcttc
     5221 aaaaatctca gcaaagacac ctacaggtgg ctgacttgtt ttcggaagat tcttactttc
     5281 ttgcatcaac tgaagaaatt taccttcttc tttcaaatcc gcatcagaat aaacaacatt
     5341 atcaatcatt acagataatg gaacaattgt aatatctaat tgctttacta gttcaggttc
     5401 aatagtaaca gatgaatcgg ttacaatctt aatttttgtc atagtatcaa tctttctatt
     5461 ttaggattca gattggtttc cttacttcta attatatcaa aaaaaagatt aaaaatccta
     5521 atggagtcaa tcaaattttc cgtaaaattt gatataatca acttataaga aaagaggtgt
     5581 cctatgatta aaaaaattta ccccattttt accattttac taggtgctgc tatttatgct
     5641 tttggactga cttattttgt agttccccat catctctttg aaggaggggc gacaggtatt
     5701 accctcatca cattttatct ttttaaaatc cctgtttccc tcatgaacct gctgattaat
     5761 attccccttt tcatcctagc ttggaagatc tttggagcca aatccctcta ttctagttta
     5821 ctaggaacct tagctttgtc cggctggtta gctttttttg agcatattcc ccttcatatt
     5881 gatcttcaag gtgatttact aatcacagcc cttatagcgg gaatcctatt gggaattggc
     5941 cttggaatta tttttaatgc tggaggtaca actggcggaa ctgatattct agctcgtatt
     6001 ctcaacaaat acactcatat atccatagga aaactgctct ttatcttaga tttttgtatt
     6061 ctcatgttga ttctcctaat cttcaaggat ttgagattgg tttcctacac gcttttgttt
     6121 gattttattg tttctcgtgt tattgatttg attggtgaag gaggatatgc cggcaaaggc
     6181 tttatgatta tcacaaaacg tcctgaccaa cttgctaagg cgattaatga tgacctcgga
     6241 agaggtgtta cttttatttc tggtcaaggc tactatagta aagaaaattt gaaaatcatc
     6301 tactgtattg tcggaagaaa tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat
     6361 cctcaagcct ttataactat tacagaagcc catgaaatcc taggagaagg cttcaccttt
     6421 gaaaaagaat aaaaagaggt aatgtcgtga cctcaaaagt tagactaaat catctatctt
     6481 ttgggttaca gacaacctct tttttgtttt atttactcaa gctcttaaga ccaattccga
     6541 gttacttctt catcagcctt taactgatcc actaattggt caactgagtc aaatttggtc
     6601 atatcacgaa tgcgatcaag ccaataaacc atgacggttt ccccataaat atcttgatta
     6661 aaatcaaaaa tattgacttc aaaacgtgct tcttctccat caaaggtcac atttttcccg
     6721 acactagcca tagcacgata cttctgtctt tgaatctcaa catcaacgac ataaacgcca
     6781 tctgctggca tataagtacg gtctaaaagc actaaattcg ctgtcggata accaattgta
     6841 cgaccacgag cattaccatg aaccaccata cctcttgatg gaagcggtgc ccccaaaagt
     6901 tttcctgctt ctttcacatt tccatctaaa atagcttgac ggatacgagt tgaactaatc
     6961 tttcctttct catcttctac aggtggaaca atgataactt ctccatcaaa gtaatccttt
     7021 aaatcttctg ctgttttttt gtcagaacca aatgtataat caaaacctgc aacaataatt
     7081 ttggcattca tagccttgat ataagttgca aagaattctt gtgcagtgag actagcgaat
     7141 tgactactaa aatcaaggag atataattct tctacacctt cgcgctttaa ttttctttca
     7201 cgttcagcag ggttcaaaat atgcaaaaac aaatccggat gataaggctc taaagcgatc
     7261 tttggagatt cattaaaggt cataacgacg ataggtaata aatcctttct cgcagccttg
     7321 ttggcaacac gaaataattc ttgatgcccc ttatgtatgc catcaaaata gccgagaaca
     7381 acgactgaat cagatggtgt gccaatatct ttttggtttt ttataggaat agtaataatc
     7441 ataaaataat tatatcatag cgatagctat ttctggaaca gaaaatctga aatgttgttt
     7501 ttttcacatg aagtgtacct gttttcaaaa agcactttat tctatcgttg cttaactatg
     7561 aactttgcaa tattcttctc aaaaacttgt aggacatctt caaaattttg caaggagtga
     7621 ttagacttgt tcggtaacca taaagtgtca tactatgtta tgtatgaaaa agcaatgcaa
     7681 ctaactcctg agaactttaa attactaatt ggtgccgaaa aggtagaatt tagaatcgag
     7741 gtacacctat ggctgtaaaa tttacaaaat gagacaactt gggcaagatg tttgaagaat
     7801 ttcctaaact ccctgatttg aagcaagtca ctttccctga tgacaaagaa aaaagccaaa
     7861 acagcaaaga aaaactagat gactgctttc caacaactcc catctagtgt gcttcagact
     7921 gggctatttt tctctccatc tgttagcttg gattctcaga ccgtttcagc taaagaatat
     7981 cttttccctt atcagaagga acggctcaaa ccattcagac aagtgaaggg acgacaagcc
     8041 aatatttgaa accagatagc agttcttata gtcaattgaa ataaaatctg aagaaatcga
     8101 gtaggaaact catatcaatt tttaacagtg ttctattcca gattcatact caatgaaaat
     8161 taaagtgcaa actaggaagt tagccgcagg ctgtacttga gtacggcaag gtgaaactga
     8221 cgtggtttga atttgatttt cgaagagtat caatccacta tacatattaa aaagtatgga
     8281 tatcaaaaca aaaaaacagg ctctccgaaa actcggaaag ccttatttta agctgtttct
     8341 agcttcctca cctttacatt tcaaggttcg ggataaaagg ttcaatggac ctttttattt
     8401 agcgattggg taaacagaaa cttgtttttt atcgcgtcct ttacgttcaa agcgtactac
     8461 gccttcaact ttagcgaaca aagtatcgtc tccaccacga ccaacgttta cacctggata
     8521 gatgtgtgta ccacgttgac ggtaaaggat tgatccacct gttacagttt gtccgtcagc
     8581 tgctttagct ccaagacgtt ttgcttgtga atcacgtccg tttgatgtag aacctccacc
     8641 ttttttgtgg gcgaaaagtt gcaagttgtt aagagtcatt tttaacataa tgttttcctc
     8701 cgtgttagtt ttctgtgata actctggttt ggacgaactc agaatagttc tccgataagt
     8761 ttgccatacc taagaaaaat gattcaaaga ataactgggt catttctctc tggtgtgaag
     8821 gaagatcttt tggtatttca accatcagat agccaccttc atcttcgttt aattctagga
     8881 ttggttcata gcctgcaaat ttctcaatag aattgataaa gttaatggca agcgtagaaa
     8941 ccgatgcaca cacgacatct aagccgtatt cgccactctc ggcgtgtcca gtaatttccg
     9001 cacccctcag ctcgccatct tcggctctct caaagactgc ttgtatcatg tgttctcctt
     9061 aaaattaagc gttaattgcg ttgatgacaa cttttgtata tggttgacgg tgaccttgtt
     9121 tacggtggct acctttttta ggtttgtact tgtaagtaac aactttcttt tgttttcctt
     9181 gtttttcaac agttccaact acagtagctc cagcaacaag tggagttccg acaacagtgt
     9241 tttcaccacc aacaagaaca acttcgttaa aagtaacttc ttgaccagct tcaacgttca
     9301 atttttcaat gtaaactgct tgaccaactt caactttaac ttgttttccg ccagttttga
     9361 taattgcgta tgtgctcatt atgcacctcc tatgattttt atgggtttcc ccgatttttt
     9421 gtgaagactc gcctagcatc gtgggacaaa ccacttaaac ttgaataatc aagcaacgat
     9481 gttcgtgcgg ttgcacagga tcgtgcatag tcaactcttc aagtatagca tatctcctat
     9541 tttcttacaa gtaataacac ctaaaatcaa gctttttctt ttactttttt ctgc
//
LOCUS       DAVBCG010000074         9536 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBCG010000074 DAVBCG010000000
VERSION     DAVBCG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.074"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            194..454
                     /locus_tag="VQ495_001425"
     CDS             194..454
                     /locus_tag="VQ495_001425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2531373.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(558..1028)
                     /locus_tag="VQ495_001426"
     CDS             complement(558..1028)
                     /locus_tag="VQ495_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2531374.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1045..3318)
                     /locus_tag="VQ495_001427"
     CDS             complement(1045..3318)
                     /locus_tag="VQ495_001427"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2531375.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLASDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            3665..6766
                     /locus_tag="VQ495_001428"
     CDS             3665..6766
                     /locus_tag="VQ495_001428"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2531376.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNEIVMKT
                     IYR"
     gene            6848..7855
                     /gene="pfkA"
                     /locus_tag="VQ495_001429"
     CDS             6848..7855
                     /gene="pfkA"
                     /locus_tag="VQ495_001429"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2531377.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            7912..9417
                     /gene="pyk"
                     /locus_tag="VQ495_001430"
     CDS             7912..9417
                     /gene="pyk"
                     /locus_tag="VQ495_001430"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2531378.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
BASE COUNT         2940 a         1649 c         2100 g         2847 t
ORIGIN      
        1 ccctttttta ttgacagtat caggatatct ttttcaaatt tttggtttgt tagataatga
       61 aaatgtttct atcaatctag atttaggatt aataaatcgt aaatgtaatt ataaagaaaa
      121 agataagtta aaaaaagatg tattagcaag agttggtcta actcatattc atctataaca
      181 aaagatatat gaattgtctg gcggtgaaac aaaacgtgtt gctttagcga aaatcattct
      241 taaaaatcca ccattaatat tcgcggatga gttgactgca tcactcgatt cgacgacttc
      301 acaaggaatt atgaaattgt tattaggtat gaaaaataaa aatcgtctaa tcatcattgc
      361 aactcataat cccgatattt ggaatcaagc agatgaagtt ataaatttaa atcaattatt
      421 acagtcaata tatatcttct atattttaaa gtaattttat ctgaatccgt taagagacta
      481 ttgtcccagg ctctttctga tgttaaagac tttctaccag gttttttaaa agcataattg
      541 ttagttgtag tcatttatta ttcttcaaag aaaaatggtg gggcgaattt tttcagttct
      601 tcaaagcact tttgagcagt atctgcatct tcacagatga taagacagac atcattacca
      661 caaagggtag cgatagcgtc agggaagctc aaagtatcaa tgatagaacc aaaggattga
      721 gccagtccag gaagggtttt tagtaggact tggtgttgaa ctgggcgcat ccagacaagg
      781 gcgtcttcca tgtagagttc gagacgtttt tcccattttg agatggaacc attgttaaga
      841 acataataag cgctatcttc ttcgcggact tttgataggt tcatattttt gatgtcgcgt
      901 gagagggttg cctgagttac ttgaatgtcg ttctcagcaa gaagggtttg caactcagcc
      961 tgtgtatgaa tcttgttttt tgtgataaga gcgcgtataa gttggtggcg gtgttctgat
     1021 ttattcataa taatgtaact ccttttagca aggtaaggta agcatggact gagcgaggtc
     1081 gacagtcaag tggtagtctg tattgtcacg gatggtgatt tcaaagtcag tagtatagag
     1141 gactaaacgg agagtgtctc cttcttttag cttgtaaata gttggctgca gttcaaattg
     1201 aacgtccatc cattcatctg cagtaatatc ctctactaac agtaaatcat ttctattttg
     1261 taaattaagg taaccttttg tcacgactct ttgtgcctct ggtctaaatg gcaattcaca
     1321 gagattttcc aacatgtgat agcgaccgtt gtcaatggtt ctagcactta aaatagctgg
     1381 ataaggttgt aggtatttct tttgcccaaa ttctagcagt tgggcagata agagcccctt
     1441 gtttgtactg gatttgatac gaagattgag ctgagcgcga ccgtttaggt ggagatcttt
     1501 agtcacagga aggttaatag taatctgatt agctttccct tgatagagct ctgtattgaa
     1561 ggtttggtat gtcttaccat agcgctcaaa atccttatct gggtactggt tttgaatagc
     1621 ttgctcttct tgaccaagtg agaaggtttc acagttttct tgcccaccga agttatcaag
     1681 tgataaccaa gtctgtggag ctgtattgtc ctgccagata acagtaggaa gttgaaagtc
     1741 tgtttcctgt cctagtaatt tcttggtcaa taaggcattt atggactcac ggaagtcaat
     1801 tgattgccaa ttgttcatgt aaacatgggc accattatgg aaaaagagat gcttgtgtat
     1861 atgagtagga agagcatgga acatctggta aacatgaagt ggtttgacat tccaatcctg
     1921 agaaccatga gtaaagacaa cctctgcctt tactttatgg gcattgagca gataattgcg
     1981 gtcatgccaa aactgattgt agtccccagt ttttcggtct agttgagctt tcactttttc
     2041 taagtcagct tggtgagctt cattgccacg gatatagtcg ctagccaaga gattacgaga
     2101 ataggttaac tcagcaaggg agtcaaagtc ctcacctgga taaccacctg ggctagtcac
     2161 cagaccgttt tcacggtagt agttgtacca tgatgaaatt cctgcctcgg caatgataac
     2221 ttctaaacca tcgactcctg tagtcgcaag accattggac atggtaccta gataggaaag
     2281 tcccgttgta gcaacttttc cgtttgacca atcagccttg acttgacgct ggcgcgtgtg
     2341 atcagtaaag gcacggcaac ggccgttaag ccaatcgatg acatttttat aagcctcgat
     2401 ttgctggtag tctccattag tcatgaaacc tgtcgagtct ttggtaccaa cacctgagac
     2461 atagagattg gcaaagcctc tcggaaggaa gtagtcgttt agtgtatagc tagagttgat
     2521 gtgagttagc ttttcctcag cctctgctat aagctcagct ttaccttggg gttggacgag
     2581 atttagttga ggtttctcta gctcaatctt gtgaggaagt ttaacctcaa gctcgccctc
     2641 catcttgtag agagccttgt cactagcctt gtcattggtt ccctgatgat aagggctggc
     2701 tgtcatgatg gcagggattt ttccatcaaa acgagggcga ataatgctaa cctttactag
     2761 gtctgatagc cctttttggt cagtatcgac acgagactca acgtaaacga cttcacgaat
     2821 gacatcctgg ttagaaaaag tagccaaact cttgccgtta aagtagtggt agtcattatc
     2881 ctccggaata agaccatcac taacaagttg gtcgataaga gtatttcctt ttttggtgcg
     2941 agtattgagt aactgataga gattttcaat caagtcacca tatataatgg gaaatccagt
     3001 ttctttacga aaaacgtcac tatcttcgaa gtcaaccaaa taagaaaagc ctaaaagttg
     3061 aaaagcaaca gtataaaaaa tatctgctgt cagttcatct tctgattgaa aaaatgtcag
     3121 caggtctgtt tttttatcag ctgctaggat agaaagtggg tagttggtgt cttgataagt
     3181 gaaaaagaaa cgacgtaaaa aggtttcaag tgagtctttg tgattggctg tattttgtaa
     3241 atcaaagcca cattttttta gttcagataa gacattttct tttggaaaat tgatataact
     3301 atattgatta aaacgcatag aacctccata tagaatgaca gttaaggtta ttatatcaaa
     3361 aaaaaagcag aaagggaatt gttaacttca aaaggaaata atccaataaa aatgaataaa
     3421 gtactaaatt caatatagag aacagagtaa caataagaat aaatagatag ggtataaaag
     3481 ttctaggaga tttatattat atgctttcta tttttatata caatatagta taaatataaa
     3541 aatgatgaca aaaatacaaa tgaatagaaa ataaattagt aagctgatga aatttttctc
     3601 aagagaagcc atttataggt gaaaatggta taatatagtg agaaggatag aggagaagtg
     3661 taaattgatc gcacaactag atacaaaaac agtctatagt tttatggaaa gcgtcatttc
     3721 gatcgaaaag tatgtgagag cagctaaaga atacggctac actcatttgg ctatgatgga
     3781 tattgacaat ctttatggcg ctttcgactt tctagagatt acaaaaaaat acggcattca
     3841 tcctttgcta gggcttgaaa tgacagtgtt tgtagatgat cagggagtga atttgcgctt
     3901 tttagctcta tctagtgtgg gctatcagca gttgatgaag ctttcgacag ccaagatgca
     3961 gggggagaaa acttggtcag tcctgtccca gtacctggag gatatcgcgg tcattgtgcc
     4021 ttattttgat agagttgagt cgttagaact aggctgtgat tactatatag gggtttatcc
     4081 agaaacacta gcaagcgaat ttcatcatcc tatcttacct ctttatcggg tcaacgcttt
     4141 tgaaagcagg gatagagaag ttcttcaagt tttaacagcg attaaagaaa atctaccgct
     4201 cagagaagtt cccttgcgtt cgagacaaga tgtctttata tcagcaagtt ctttagagaa
     4261 actattccaa gagcgttttc cgcaagcttt ggacaattta gaaaagctta tttcaggcat
     4321 ttcttacgac ttggatacta gtctgaaact gcctcgtttt aatccagcta gaccagcagt
     4381 agaggagttg agagagcgtg ctgaactggg gcttgttcag aaggggttga ctagtaaaga
     4441 atatcaagat agactagacc aagaattgtc tgttattcat gatatgggct ttgatgatta
     4501 tttcttggtt gtttgggatt tgttgcgttt tggacgatcg aatggctatt atatgggaat
     4561 gggaaggggt tctgcagtag gcagtttggt ttcttatgcc ttagacatca cggggattga
     4621 cccagtagag aaaaatctga tttttgaacg ctttcttaat cgtgaacgct ataccatgcc
     4681 tgatattgat attgatatcc cagatattta tcgtccagat tttatcagat atgttggtaa
     4741 taaatatggt agtaaacatg cggcacaaat cgttactttt tcaacctttg gagccaagca
     4801 agctcttcga gatgtcttga aacgctttgg tgtgccagag tatgaattat ctgcaattac
     4861 taagaaaatc agttttcgtg acaatcttaa gtcggcctat gagggcaatc tccagtttcg
     4921 tcagcaaatc aatagtaagt tagaatacca aaaagctttt gagattgctt gcaagataga
     4981 gggctatcca aggcaaacct ctgtccatgc ggctggtgtt gtaattagtg accaagattt
     5041 aaccaactac attcctctaa agtatggtga tgaaattcca ctgactcagt atgatgctca
     5101 tggagttgag gctagcggac ttttgaagat ggactttctg ggactacgaa atttgacctt
     5161 tgtccagaag atgcaagagt tgcttgctga aacagaaggt attcatctga aaattgaaga
     5221 aatcgattta gaagacaaag aaacattagc tttatttgcc tctggtaata caaaaggtat
     5281 ctttcaattt gagcaaccag gtgccattcg tctgcttaag cgtgtgcaac cagtctgttt
     5341 tgaagatgtc gtcgcgacta cttctctaaa tcgaccgggt gctagtgact atatcaataa
     5401 ttttgtggca agaaagcatg ggcaggaaga agtgactgtt ctggatccag tactggagga
     5461 tattttggct ccaacctacg gcataatgct ctatcaggag caggttatgc aggttgccca
     5521 gcgacttgcc ggatttagtc ttgggaaagc cgatattttg cgtcgggcta tggggaaaaa
     5581 ggatgcctct gccatgcatg agatgagggc ttcctttatt caaggttcat tagaagctgg
     5641 tcatactgtg gaaaaagcag agcaggtctt tgatgttatg gagaagtttg caggttatgg
     5701 ttttaacagg tcacacgcct atgcctactc agccttggcc ttccagttgg cttatttcaa
     5761 aacgcattat ccagccattt tttatcaggt catgttaaat tcttccaaca gtgattactt
     5821 aatagatgca cttgaagcag gttttgaagt agcctctcta tccatcaaca ccattcccta
     5881 tcacgataaa attgccaaca aggccatcta tctaggtttg aaatccatta aaggagtcag
     5941 taatgattta gctctctgga ttattgaaaa tagaccttat tctaacattg aagattttat
     6001 agctaaatta cctgagaatt atctgaaact tcctctgcta gaacctttgg taaaagttgg
     6061 tcttttcgat tcatttgaaa aaaatcgtca aaaagtattt aataacttag ctaatctatt
     6121 tgaatttgtg aaagagttgg gaagtttgtt tggagatgct atttatagtt ggcaggaatc
     6181 ggaagattgg acggaacaag aaaaatttta tatggaacaa gagcttttag ggataggtgt
     6241 cagcaaacat ccactacaag ctattgcaag taaggctatt tacccgatta ccccaatcgg
     6301 aaatttgtca gaaaatagct atgctattat cttggttgaa gttcagaaaa taaaagtgat
     6361 tcgtaccaaa aagggtgaaa atatggcctt cttacaggca gatgatagta agaaaaaatt
     6421 ggatgttaca ctcttctcag atttatatcg acaggttggt caggaagtaa gagaaggagc
     6481 attctactat atcaaaggga aagtccaatc acgtgacggt cgtctgcaaa tgattgcaca
     6541 agaaataaga gaagcagttg ctgaacgctt ttggatacag gtgaaaaatc atgaatcgga
     6601 tcaagaaatt tcacgtattt tagaacaatt taaaggccca atcccagtca tcatccggta
     6661 tgaagaggaa cagaaaacca tcgtttctcc ccatcatttt gtagctaaat ctgatgaatt
     6721 agaggcgaaa ttgaatgaaa tcgttatgaa aacgatttat cgctaaaaat acggaaaata
     6781 gaagaatttt aaaatcaaat gtgatataat caagaagaat gttaaaagaa aaaggagcat
     6841 aaccaatatg aaacgtattg ctgttttgac tagtggtgga gacgcccctg gtatgaacgc
     6901 tgccatccgt gcagttgttc gtcaagcaat ttcagaagga atggaagtgt ttggtatcta
     6961 tgacggatat gctggtatgg ttgccggtga aattcatccc ctagatgcag cttcagtagg
     7021 ggacatcatt tctcgtggtg gtactttcct tcactcagct cgttacccag agttcgctca
     7081 acttgaaggg caacttaaag ggattgagca attgaaaaaa cacggaattg aaggtgtagt
     7141 tgttatcggt ggtgacggat cttaccacgg cgctatgcgt ttgactgaac atggcttccc
     7201 agctattggt cttccaggta caatcgataa cgatatcgtt ggtactgact ttacaatcgg
     7261 ttttgacaca gcggttacta ctgccatgga cgctatcgat aagattcgtg atacatcatc
     7321 aagtcaccgt cgtacttttg taatcgaagt tatgggacgt aacgctggtg atatcgctct
     7381 ttgggctggt attgcaactg gtgctgatga aatcatcatc cctgaagcag gcttcaagat
     7441 ggaagatatc gtagcaagca tcaaagctgg ttatgaatgt ggtaaaaaac acaatatcat
     7501 cgtcttagct gaaggtgtga tgtcagcggc tgaatttggt caaaaactta aagaagctgg
     7561 agatacaagc gaccttcgtg taacagaact tggacatatt caacgtggtg gttctccaac
     7621 tgcgcgtgac cgtgttttgg cgtcacgtat gggtgcacat gctgttaaac ttcttaaaga
     7681 aggtatcggt ggtgttgcgg ttggtattcg taacgaaaaa atggttgaaa atccaattct
     7741 tggtactgca gaagaagggg cattgtttag ccttactgca gaaggtaaga ttgtggttaa
     7801 caacccgcac aaagccgata ttgagctatc tagcttgaat aagagcttgt cataatttac
     7861 aatttagtta ataagaccaa aaaggtcata tataaaggag tcacaaaaat catgaacaaa
     7921 cgtgtaaaga tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg taaaaaattc
     7981 ggtgaggacg gatactgggg tgaaaaactt gatgttgaag cttcagctaa aaacattgct
     8041 aaattgattg aagctggtgc taacacattc cgattcaact tctcacacgg cgaccaccaa
     8101 gaacaaggtg agcgtatggc aactgttaaa cttgcggaaa aaattgcagg taaaaaagtt
     8161 ggtttccttc ttgatacaaa aggacctgaa atccgtacag aattgttcga aggtgaagct
     8221 aaagaatatt catacaaaac tggtgaaaaa attcgtgttg caactaaaca aggaatcaaa
     8281 tcaactcgtg aagtgattgc gttgaacgtt gctggtgctc ttgatatcta tgatgatgtt
     8341 gaagttggtc gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt ggttgctaaa
     8401 gatgatgcaa ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat cgctaaacaa
     8461 aaaggtgtga acatccctaa cactaaaatt cctttcccag ctcttgctga acgcgataac
     8521 gacgatatcc gtttcggtct tgaacaaggt atcaacttca tcgcaatttc attcgtacgt
     8581 actgcaaaag atgtgaacga agttcgtgca atctgtgaag aaactggaaa cggacatgtt
     8641 caattattcg ctaaaatcga aaaccaacaa ggtatcgata acttagacga aatcatcgaa
     8701 gcagctgatg gtattatgat tgctcgtggt gatatgggta tcgaagtacc gttcgaaatg
     8761 gttccagttt atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa agttgttatc
     8821 actgcaacaa acatgcttga aacaatgact gaaaaaccac gtgcaactcg ttcagaagta
     8881 tcagacgtat tcaacgctgt tatcgacgga actgacgcta caatgctgtc aggcgagtct
     8941 gcaaacggta aatacccact cgagtcagta actacaatgg ctacaatcga caagaatgct
     9001 caagctcttc ttaatgaata cggacgtctt gattcagatt catttgagcg taactctaag
     9061 acagaagtaa tggcttctgc tgttaaagat gctactagct caatggatat caaattggtt
     9121 gtaactctta ctaagacagg tcatactgca cgtttgattt ctaaataccg tccaaatgct
     9181 gacatcttag cattgacatt tgacgaattg acagaacgtg gcttgatgtt gaactggggt
     9241 gttatcccaa tgttgacaga tgctccatct tcaactgacg atatgttcga aatcgctgaa
     9301 cgtaaagcgg tagaagcagg tctcgttgag tcaggcgatg atatcgttat cgttgctggt
     9361 gtgccagtag gagaagctgt tcgcacaaac acaatgcgta tccgcacagt acgttaagaa
     9421 aaatataaaa acctatcata tccagcttta gagcttgtgt gataggcttt ttgtatagag
     9481 ggtaagaaat aggcaaaact ttcataatgg attgatactc ttcgaaaatc aaattc
//
LOCUS       DAVBCG010000075         9400 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBCG010000075 DAVBCG010000000
VERSION     DAVBCG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.075"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(88..1224)
                     /gene="dnaJ"
                     /locus_tag="VQ495_001431"
     CDS             complement(88..1224)
                     /gene="dnaJ"
                     /locus_tag="VQ495_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2531379.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1429..1782)
                     /locus_tag="VQ495_001432"
     CDS             complement(1429..1782)
                     /locus_tag="VQ495_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531380.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(1795..3618)
                     /gene="dnaK"
                     /locus_tag="VQ495_001433"
     CDS             complement(1795..3618)
                     /gene="dnaK"
                     /locus_tag="VQ495_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2531381.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4098..4622)
                     /gene="grpE"
                     /locus_tag="VQ495_001434"
     CDS             complement(4098..4622)
                     /gene="grpE"
                     /locus_tag="VQ495_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2531382.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4649..5683)
                     /gene="hrcA"
                     /locus_tag="VQ495_001435"
     CDS             complement(4649..5683)
                     /gene="hrcA"
                     /locus_tag="VQ495_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2531383.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(5847..6356)
                     /locus_tag="VQ495_001436"
     CDS             complement(5847..6356)
                     /locus_tag="VQ495_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2531384.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            6964..8902
                     /locus_tag="VQ495_001437"
                     /pseudo
     CDS             6964..8902
                     /locus_tag="VQ495_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            8900..9400
                     /locus_tag="VQ495_001438"
                     /pseudo
     CDS             8900..9400
                     /locus_tag="VQ495_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         2750 a         1918 c         1830 g         2902 t
ORIGIN      
        1 gactcacata tatacggcaa gccgacgctg acgtggtttg aagagatttt cgacgctgac
       61 gtggtttgaa gagattttca aagagtatta ttctccatca aaggcatctt taatatggtc
      121 aaagaagcct tttttctttg gatttacttt caagtcacca gcagccgcga attctttcaa
      181 ggctactttt tggcggtcgt tcaagcctgt cggtgttacg acattaacag taacgtattg
      241 gtcaccaact gcaccgccac gaaggctcgg tgccccctta ctacgtaggc ggaatttctt
      301 accagtctga gttccctctg gaataaccaa ttcaacatca ccgtgaacag ttggaatatc
      361 tactgtatca ccaagagccg cttggacaaa gttgaggttg agattgtaga agatagtcgt
      421 tccttcacgt tcaaacttgt cgctagcttc tacagaaact actacataca agtccccata
      481 aggtccaccg ttaaagcctg cttcgccttg accagcgagg cgaatttgtt gacctgtttc
      541 cacaccagca gggattttca catgtacgct atgagcttgt ttctcatgac ctgttccatg
      601 acaggttgta catggatatt tgatttcttt tcctcgaccg tgacagacat cacaggttac
      661 ttggcgacgc atcataccaa gaggagtctg cgtatcgacg ttaatgacac cagcgccatg
      721 acagcgtcca caagtgactg gacttgtccc tggcttagca ccagagccat tacatgtacg
      781 acagccagct tcacgatgat acttaacttc cttctcagtt ccgaagatag cttcttcaaa
      841 ggtcaaattg acacgatact ggagatcatc tccttggcga ggagcgtttg gattgcgcga
      901 agaaccgcct ccgccgaaga aacttgaaaa aatatcctca aaaccaccga agccacctgc
      961 cccattgaaa ccaccgaaac caccagctcc accaaaacca ccattggcgc ctgcagcacc
     1021 atactggtca taggcagcac gtttttggtc gtcactcaaa gtctcatagg cttcttgaac
     1081 ttccttgtac ttgtcctcag caccaggctc cttgttgata tctgggtgat attttttgga
     1141 aagcttacga taagcctttt tgatctcgtc tgccgaagcg ttttttgaca cccccagacg
     1201 atcataaaat tcagtattgt tcatacaaga taccaagacc gtaaaattcg actgaaaaat
     1261 aggaaatctg acgctggagc gacgctccta gacagatttc tcttttttcc gagaatttag
     1321 gtcgggttca attcctttct tttatattga tgagataata ctccagccac tcggccctat
     1381 ccctttttac cgaaaaacag aggctgagtt gcaacctctg tggttagatt attcatttga
     1441 aataaaaatg gttgtattat ctctagtagt aatcttcaaa gaccttttag ctaaatcaaa
     1501 ctcaaattta ttatagtgtg ggtcttgtgt tttatatagg tgtaactcct gacatatatg
     1561 atccgaaaag taaatcataa catctttaaa cacagaagtg tggtcatatt tactcttacc
     1621 aacagctact tctgtaaatt ctttataaat gacatttcca tttctttcca cagtcaccag
     1681 catttctgtt ggattataat ccaaattaaa taattcttca aaaaacttta tcttttcatg
     1741 ataatctgca atgtcagaaa gattattttt attaacactc ataacactag ccccttattt
     1801 ttcagtaaac tctccgtcta cgacgtcatc gcctgcgttt cctgttgctt gtgcgccttc
     1861 tgctccttct tgagcttgtt gcgctgctgc agcttgttcg tagagtttaa cagcaagtcc
     1921 ttgagctttt tcgttcaatg cttcaagttt tgctttcatg tcgtccaagt tgttgtcttc
     1981 ttgagctttc ttaaggtcat caagggcagc ttgggcagcg tcacgttctg cgtcgaagcc
     2041 tttaccttca gtttccttga ttgtcttttc agtcgcaaag attgcttggt ctacttcatt
     2101 acgaaggtct acttcttctt tacgtttctt atcggcttca gcgtttgctt ctgcatcttt
     2161 catcatgcgg tcgatttctt cgtcagtcaa acctgagttc gattggatga caatagtttg
     2221 ttctttttga gttccaaggt ctttggcctt aacagacacg ataccgttct tgtcgatgtc
     2281 aaatgttact tcgatttgag gaattccacg aggtgcagct gggatatcag tcaattggaa
     2341 gcgtccaaga gtcttgttat ctgctgccat tgggcgttca ccttgaagaa cgtggatatc
     2401 aacggctggt tggttgtctg ctgctgttga gaagacttgt gatttagatg ttgggattgt
     2461 agtgttgcga tcgataagtt ttgtaaatac tccacccatt gtttcgatac caagtgacaa
     2521 tggcgttaca tcaagaagga caacgtcctt gacatcacca gtaatcacac caccttggat
     2581 agccgcaccc atagcaacta cttcatcagg gtttactgat ttgtttggtt ctttaccagt
     2641 ttcagcttta acagcttcaa caacggcagg gatacgagtt gaaccaccaa caaggataac
     2701 ttcgtcgatt tctgacaagc tcaaacctgc atctgaaagg gcttgacgaa ctggaacttt
     2761 tgtacgttca acaaggtcac gagtcaaatc gtcaaatttc gcacgagtca aagtcatttc
     2821 caagtgaaga ggtccagcct cacctgcagt gataaatggc aagctgattt gtgttgaagt
     2881 tacaccagaa aggtctttct tcgctttttc agccgcatct ttcaaacgtt gcattgccat
     2941 cttgtcagta gacaagtcga taccgttttc tttcttgaat tctgctacca agtggtcaat
     3001 gattttttgg tcaaagtcgt caccaccaag tttgttgtcc cctgcagttg acaatacgtc
     3061 gaagacaccg tcacccaatt caaggataga gacgtcgaat gtaccaccac caaggtcaaa
     3121 taccaagatt ttttcttctt tgtcagtctt gtccaaacca taagcaagag ctgctgcagt
     3181 tggttcgtta acaatacgtt ctacttcaag accagcaatt ttaccagcgt cttttgttgc
     3241 ttgacgttga gcgtcgttga agtaagccgg aactgtgata acagctttgg ttactttctc
     3301 accaaggtag tcttcagcgt agcctttcaa gtattgaagg atcatagctg agatttcttg
     3361 tggagtgtat tcttttccat ttgcagaaac tttttcagaa gttcccatct tagatttgat
     3421 agagataact gtatctgggt ttgtaactgc ttgacgtttt gcagcatcac caacgatgat
     3481 ttctccgttt ttgaatgaga ctacagatgg agttgtgcgg tttccttctg ggtttgcgat
     3541 gattttgctt tcagttcctt caagaactgc aactgctgag tttgttgtac ctaagtcaat
     3601 accgataatt ttagacatgt gtttttctcc ttgttagtta attttctatt tttctttttg
     3661 atactcttct taagtggcga cgccgtcaga gcttgacttc gtcaatctct ttgactaaac
     3721 tttgagccta aggtctcaaa atttgcgcaa atagcaccac ggcgaagagt atcgtctttg
     3781 gttcgcttcg ctcactatta caaagccaaa catattttat ctttcttcat agtttattgt
     3841 cgtttcggac aagttttcta ttatgtaaat tgcgacacga ggagtcgaaa tcgattttat
     3901 ttcgacgacg agttagtaag gaagctaggc aaacgtcata gcgattgccg ttttctgacg
     3961 agttgcttta gctaccgtca gaattgccga atcgaacacg ccctaagctc tgagtaaaaa
     4021 agatagattt tcttgtgttc atcgaacact tcgtcaatct cctattttta ctgcgagctt
     4081 tttacgagct ttgtatctta gttatacacc actaccattg ctgggcgtag gatgcggtca
     4141 tggagtttgt agcctttttg aaagacttgg gcgatggtat ctactgggtg ttcatcgtct
     4201 gctgggagag tttggatggc catatggtag ttatggtcaa attcgccatc tgctgcgatt
     4261 tcttcaattc cttcttcttt caaagcgtga atcaagcttt cttgcaccat ccccaagccc
     4321 ttcttcacat catctgtcaa accttcaact gcaagtgcac gctcaaggtt gtcaagagat
     4381 ggtaagattg cttttgccaa gtcctggcta cgataacgtt gcaagttttg acgttcttca
     4441 ttggcacggc gttggatatt ttgcatttct gcatgagcgc gaagatattt gttttcgaac
     4501 tcatctgcac gttcatttgc caagtccaac tcagactttt caggagttgt ttcttcagct
     4561 gtttccacaa cttcctcttc ttgaacttct tctacttctt cattttttat atcttgggcc
     4621 atgttcgcct cctttaatga tttcaatctt aatgtacttc gtaatgatta ctgctgaggt
     4681 agcggtaaaa atctgtcaac ttcatggtca aaacacggtt gaccacattg acttgattga
     4741 ttagctgttg gtaatccaga ttaactggac cgataatggc tagaattcca actccccgat
     4801 aaggaatgag gaacttacta ctaatcaccg ctaggtcagc taaacaggac tcttgaccgt
     4861 ctgcaacacg aacattttgc atctgatcct cacgcaaccc ctcacgaatc tccaaggcca
     4921 ccttttgcgg ttggtcaaag aactgatagg cttctagatt ggcaaaattc aagagattga
     4981 ccttgcccgc catcacaatg ttttcgttga acatttcctt aaagatgtgt tcaaagagat
     5041 cgatgacatt atccgttgtt gtaaagtaac gctggataat ctgcggaatc tccgtccgaa
     5101 tcttgtagtg aatatctaaa acggtgtgac cgaggaaacg ttcctgaatg atgctcttca
     5161 gtttcagcaa atcctcctgc aagaagttcc ttggaatcag aaactgacta gtaaccgttc
     5221 gcgactcgtc tagggtaaat accgccaagg ctgtatgttg ccccaaaaca acgatatcaa
     5281 aggctgtcaa acgttgcctg ctcggctcaa catccagtgc cactaccgta cagccactca
     5341 ggcctgttag caagttagca gcctcttgca gaatatcctc caatttgaag aattcctgat
     5401 caaaggcttt gacaatctca tatacctcat tttcagccag ccggtgaaaa tccagtgagt
     5461 gtttcacata gtactgaaaa ccagcaacac ttggcatccg accgcttgaa gtatgagcct
     5521 tctcaagcaa cccttgtttt tctagttccg ccatgtcatt acgaatggtt gcactgctag
     5581 agttaataga ctcttgcaag gcttttgatc cgacaggttc gtgcgttttg gtaaagatgt
     5641 caataatcag atttaaaata tcctgctgac gctctgtaac catctcatca ctcctctctg
     5701 tctgatcaat tagcactcaa tgcatccaag tgctaactta tgattctatt atacaccctt
     5761 aaaagagaat gtcaagacaa aaactcaaaa aattagcact cttttatcaa gagtgctaaa
     5821 aatcaatctg aagacctaga accttgctat tcatctactc ggtatttcct ttttagtctg
     5881 aaataaccat gaattagata agaaatcaga aaagcatata aaacaaaaat aagaaagaaa
     5941 gacatagaaa gatcctctct aaacaaactc gtacaaacaa caagaccacc taagaaagta
     6001 gttgtacagg aacgaagtct agatcgcata acttcccatt tgagcccctt actcatatag
     6061 acttgatcct ctggaagtaa atcagaagac gatttacgaa gaatcgaaca agaacctgct
     6121 cctatcaatt cccaacctct atctctaaaa tcttgcagtt catgcttata ttttttaaga
     6181 aatctagaat catagatacg gtagatgaca tcgtctggtt gacattggtc aaaatagaac
     6241 aaaccaaaac gactcgttct atacctccaa cctttcaaat gcatctcatg taaatattct
     6301 tcttccttgt ccaaatcaac aatggtgaaa atccgaaatt ctactctgct attcattgtc
     6361 ttaccccaaa attagaaaac atgcctggcg ttatttatta gataattctt tccacttttg
     6421 actcaatctc caaaaaatat aagaaatctg aatcgcaaaa actatcaata aaacccaatc
     6481 tattatgaaa atcaaaaaca ctttccaact gaaagaacta cctccagtga caaactttga
     6541 gcaaaacggt agtagagcta aaaagagaag taaaatagga agcatccgca ttgttaaaat
     6601 ccgtttggca taaaaaaatc tttatttaaa ctaaaatatt atagcaaaat ttacgccagt
     6661 ttttgaacgg ctgatgtaga tattttatac tttcaaaatg tttaaatgtg attatttatt
     6721 tttgaaaaat agctcaccag cccgactgaa agtgcttata gaatgataat aagtcgcctg
     6781 ccgaaaacag cggaaaatag cggtgttatg cggagataat ctgacgcgat gcgaaagtat
     6841 attgcatact tattttcaac aatttagcag agtattttta taagtgtgat ataatagaag
     6901 tataatttgt tctgatagtt tattttatgg agaagtagat ttttagaatg cggagggttc
     6961 aatatggttg agtttataaa gtctaagaaa gaaatgagtg aggaggatat taaagcaaat
     7021 ttcatcactc ctgctattgt atccaaagga tggaaaaatg gtgagcatat cgcttacgaa
     7081 gaatacttca ctgatggtcg aattgaggtt agaggagata aggctcgtcg taaagaagga
     7141 aaaaaatcag actattcact gtattaccaa tttggaactc gaattgcaat tgttgaggca
     7201 aaggataata aacacagcgt tcgagcagga ttacaacaag ctattgaata tggagagatt
     7261 ttagatgttc catttgttta ttcttcgaat ggtgatggct ttattgaaca cgaccgtatc
     7321 acgagagaag aacgtgagct ggagttagac gaattcccta ctcgtgaaga attattttct
     7381 cgtatgacga aggaaaaagg attgacgtac gaaattacag aagctatctc aactccatac
     7441 tatacagacg ccttctcaat gaaaacgcca cgctattatc agcaaatagc tatcaaccgt
     7501 actattgaaa cagttgccag aggacaaaaa cgagtaatgt ttgtgatggc aacaggaaca
     7561 gggaaaacgt tcatggcttt tcaaattatt catcgccttc gaaaagctgg tttggctaaa
     7621 cgagttttat tcttagcaga tagaaacatc ttagtagacc aaacgatggc tgaagacttt
     7681 aagccattcg aaaaggtaat gacgaaaatt acaccaaaac ttttgactgc tcctgaaaaa
     7741 ttaaattctt tcgaaattta tctagggctt tatcagcaac taactggtga agatggaact
     7801 gaaacacatt atcaaaaatt tgacaaagac ttctttgatt taatcgtaat tgatgaagcg
     7861 caccgtggtt cagctaagga aaacagtaac tggcgtaagg taattgatta tttcagttct
     7921 gcgacacaga ttgggatgac cgctactcct aaagaaacca agaatgcttc caatacggaa
     7981 tactttggta agccaatcta tacttatagt ttaaaacagg gaatcgagga tggttttttg
     8041 gctccatatc gtgttatgag ggttaattta gatgtggatg tggatggtta tcgtccagaa
     8101 actggaaaag ttgatgctaa cggacaatta atagaagata ggtactacgg caggaaagat
     8161 tttgataaaa ccattgtcat tgatgataga acgcaaagag ttgccaagtt tgtttctgat
     8221 tattatgaag caaaacaatg cacgatttga taaaacaatt gttttttgtg ttgatattga
     8281 ccatgccgag cgaatgcgtg ctgcacttgt aaaagagaat ctagacttag tccaagaaga
     8341 ctatcgttat gtcatgcaag taactggtga caacgctgaa ggaaaagctc aactggataa
     8401 ctttatggat gtcaattcta attttcccgc tattgtaaca acgtctaaat tattaacgac
     8461 aggagttaat gctaaaacat gtcgtttgat tgttttagac tctaatatcc aatccatgac
     8521 tgaatttaaa caaattattg gtcgtggcac acgtctttat cctcaaaagg ggaaagaatt
     8581 ttttacgatt attgattttc gaaatgttac caatttgttt gctgaccctg attttgatgg
     8641 tgagccagtg aaggtgctgg aaacaggtgc gaaaacagtc agtggttcta cgcctggttt
     8701 cgtaggtgag gaaggtgacc cagtagaaaa atatatcgtt acagacaagc aggttaccat
     8761 tcttaattct actgttcaag tattggatga aaacgggaaa ctgattaccg aaagcctgac
     8821 cgactacact cgaaagaata tcttaggtag ctacgccact ttgaacgatt ttatcacagt
     8881 ttggcatacg gcagataaga aacattatgg gaagtttgct gatggaagca ctcaagaaat
     8941 tgatgttcct tatgatattc ctgatacttg ggagtgggtg aggttttcta cattggttga
     9001 aattgtcaga ggtggctctc cacgaccaat caaagattat cttacttctg aagtagatgg
     9061 aataaattgg ataaaaatag gtgatactga aaagggtgaa aagtatataa ataatgttaa
     9121 agaaaaaatc aaaaaatcag ggcttaacaa aactagattt gtaaaaaaag gtacattttt
     9181 gttaactaat tctatgagtt ttggtagacc ttatattttg aatgttgatg gtgcaataca
     9241 cgatggatgg ttggctattt cgaactatga aaactcatta aataaagatt acctattcta
     9301 tattctttca tcaaatgtag tttattctca atttctatct ctaattagtg gagctgttgt
     9361 gaaaaacttg aatagtgata aagttgcttc tattcttatc
//
LOCUS       DAVBCG010000076         9376 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBCG010000076 DAVBCG010000000
VERSION     DAVBCG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.076"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(89..493)
                     /locus_tag="VQ495_001439"
     CDS             complement(89..493)
                     /locus_tag="VQ495_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2531385.1"
                     /translation="MKTFFAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGLKYYKK"
     gene            complement(490..654)
                     /locus_tag="VQ495_001440"
     CDS             complement(490..654)
                     /locus_tag="VQ495_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531386.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            complement(953..1072)
                     /locus_tag="VQ495_001441"
     CDS             complement(953..1072)
                     /locus_tag="VQ495_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2531387.1"
                     /translation="MDFKSFIIGLVVGIFGPYMYNLIRKIFSKFPKKDTDSTP"
     gene            complement(1075..1305)
                     /gene="blpU"
                     /locus_tag="VQ495_001442"
     CDS             complement(1075..1305)
                     /gene="blpU"
                     /locus_tag="VQ495_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2531388.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(1374..1643)
                     /gene="blpJ"
                     /locus_tag="VQ495_001443"
     CDS             complement(1374..1643)
                     /gene="blpJ"
                     /locus_tag="VQ495_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2531389.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(2110..2307)
                     /gene="blpI"
                     /locus_tag="VQ495_001444"
     CDS             complement(2110..2307)
                     /gene="blpI"
                     /locus_tag="VQ495_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2531390.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            2589..4742
                     /gene="blpA"
                     /locus_tag="VQ495_001445"
     CDS             2589..4742
                     /gene="blpA"
                     /locus_tag="VQ495_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HET2531391.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLVIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            4753..6114
                     /locus_tag="VQ495_001446"
     CDS             4753..6114
                     /locus_tag="VQ495_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2531392.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLNQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            6171..6299
                     /gene="blpC"
                     /locus_tag="VQ495_001447"
     CDS             6171..6299
                     /gene="blpC"
                     /locus_tag="VQ495_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2531393.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(6370..7710)
                     /locus_tag="VQ495_001448"
     CDS             complement(6370..7710)
                     /locus_tag="VQ495_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2531394.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEGSQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(7724..8461)
                     /locus_tag="VQ495_001449"
     CDS             complement(7724..8461)
                     /locus_tag="VQ495_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2531395.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(8466..8804)
                     /locus_tag="VQ495_001450"
     CDS             complement(8466..8804)
                     /locus_tag="VQ495_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2531396.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            9081..>9376
                     /locus_tag="VQ495_001451"
     CDS             9081..>9376
                     /locus_tag="VQ495_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2531397.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKT"
BASE COUNT         3124 a         1937 c         1457 g         2858 t
ORIGIN      
        1 ataccatatt ttgtttcgca gggaatctat tataaaaggg taagtattgc aaaaacactt
       61 acccttttct tttatacttc attaagctct actttttata atacttcaag ccccacatga
      121 gcagaagcat gatgattaag cagagaacag cgccaatata agcgattatt tgttggtagg
      181 attctcctgc tgtgatacct ctatacaaac aaataataga cataaaacct gtcaagccga
      241 tgaacataag ttgattggtt ctaggactaa ccaaatcatc atcttcaaac tctcttatcc
      301 tcatttccct agtgagataa acagtaacca aaatagaagc caagttaata actactaaaa
      361 gaaattggaa aactacggaa aaatttaaaa actgacgaga tagaaataga taagtagaaa
      421 caagcaaggg caactgacct aagaacaatc tcgcaaggaa gatgttccgt tttttagcaa
      481 aaaaagtttt catttctttt ctcctttctt tttattgata gcaaaataga tcataactgc
      541 aatcacatag gctatggtat aaaatagctg ataccaagca ctctccctaa gcggatatag
      601 aaagatggac atgattagat acagaacgaa aataatcagt atttttttct tcataagatt
      661 tcctcctaaa tgtgcgattt atcttagttg agcaagaaca tttacactgc tagtatagca
      721 cttattttga ccttggatca ctcaaatcat aaatggtcat cgaaacatct tgaattgtta
      781 aaaaatttaa aaagtaagca ttaaaaacat actttcctct ttatattgta ttgataccaa
      841 cttgtttgta gacttttcat cctgctatca catatcattt tgacaggcga aacaaaatta
      901 aagaaactcc cctgtaaatt aagctagcaa atacagggga gaaatttatt ttttaggggg
      961 tactatctgt atcctttttg ggaaattttg aaaatatttt tctaattaaa ttatacatat
     1021 aaggaccaaa tataccaact actaaaccaa taataaaact tttaaaatcc ataattacca
     1081 ccaacatgtt gctgcatagg ctacacctcc aagtatagct ccacccgcag caccagttgc
     1141 tgcaccttgc catgttcctg ttttaatgcc tagttgaaga cctcttgctg ctgctcctcc
     1201 aacacctgct ttggcaaaat ctccccaatt gcatccgcca ccttcaacgc aagcaagcat
     1261 ttcagtatcc ataacagaaa attgtgacat catttttgta tccatgacaa atactccttt
     1321 tttaaaaaac taaaataaat cagaatagaa tcctcataat tttactataa gtcttaccaa
     1381 cttagtccca atttatcacc aaccatacct cctaagcatg ttaatccacc cccaattgca
     1441 ccaatgtgtg ctccaacaaa tgcaccagca agtccagcta ctcctaaagt ggccaaacct
     1501 gctccagttc caccagttat aattcccgta gtgactcctg taatcagtgc attttgacaa
     1561 tcagtggagc tatacccccc ttcaactttc gcaagcattt cagtatccat aacctctaac
     1621 tgtgacaaca tttttgtatt catgatgaat acctcctttt tattttcaat ttgttaccaa
     1681 agtcttaaat tcaataaaca aatagatttt ttatagtatc tttttgattt tcttaaaaaa
     1741 gtatatacgt ctactatctt cttaaaggta gcagtaccta ttttttagtc taagatttca
     1801 ataatcttga gtatctaaaa tatcttaatt tcgttattct ccttgcaata aaaagtttta
     1861 ctatactatt tattaacttg cagaaagcaa aaaatattag taaataatag tttatagtta
     1921 agttttttat tcctaccaat ccatcaacta agtaaaacat caacgattac ataaacgatt
     1981 gataatataa ttaaaatttt gctaactatc ttattctcat cattcttaga taactttgat
     2041 attttgtaag taagtaaata agacagtaaa ttaatagcga taataatact atatttaaga
     2101 atcataatct tacaaagagg acataattcc tgaacctaca caaataagtg ttgctgctcc
     2161 cccagttatc ggaccagtcg cagcagctaa tagtactgct ccaatacaac caccgattgc
     2221 agatcctaaa ttgcctcttc ctccactaac tatttcgagt tcttcattat ccataacaga
     2281 aaattgttcc atcatttttg tattcatgac aaatactcct tttttctttt tttatttttg
     2341 tcttgttgta actttgataa gtttagtata tcatcgtttt ttaaaatttt tcatccagat
     2401 cttgaattgt catcgaaacg tcttgaatta gcttttttat ttcaagccac ctctaaatgt
     2461 ttaaaaaaaa taatttctaa tcactttttt accattcagg aagttttaat gactattcaa
     2521 gatttcataa aatatgaact tagttttatg acataataga cctatccact atatgaaaag
     2581 aattgccaat gacttcttat aaacgtacat ttgttcctca aatagatgcg agagactgtg
     2641 gtgtcgctgc cttagcctcg attgctaaat tctatggttc agatttttct ctagctcact
     2701 tgagagaact tgcaaagacc aataaagaag ggacgactgc tcttggcatt gtaaaagccg
     2761 ctgatgaaat gggctttgaa acaaggcctg ttcaagcaga taaaactctc tttgacatga
     2821 gtgatgtccc ctatccattt atcgttcacg ttaacaaaga aggaaaactc caacattact
     2881 atgttgtcta tcaaacaaag aaagactatc tggttattgg tgatcctgac ccttctgtaa
     2941 aaatcactaa aatgtcaaaa gaacgctttt tctctgaatg gactggagta gctatttttc
     3001 tagctcccaa acccagctat caaccccata aagataaaaa gaatggtcta ctaagcttcc
     3061 ttcctctgat tttcaaacaa aaatctctca ttgcttacat tgttctctca agcttattgg
     3121 tcactattat caatataggt ggttcttatt atctccaagg aatcttagat gaatacatcc
     3181 caaatcagat gaaatcaact ttaggaatca tctcagttgg tctggttatc acctatatcc
     3241 tccaacaagt catgagcttc tccagagatt atctcctaac cgttctgagc cagagattaa
     3301 gcattgatgt gattttatcc tatattcgcc atatttttga acttcccatg tctttctttg
     3361 cgacacgtcg tacaggagaa atcatttcac ggttcacaga tgctaactct attatagatg
     3421 ccttggcttc taccattctt tctcttttcc tggatgtttc tattctgatt cttgtagggg
     3481 gcgtcttact ggcacaaaac cctaatctct tccttctttc tcttatttct attcctatat
     3541 acatgttcat catcttttct tttatgaagc ctttcgaaaa aatgaaccat gatgtcatgc
     3601 aaagtaattc tatggttagc tctgccatta tcgaagatat caacgggatt gaaactataa
     3661 agtcgctcac gagtgaagaa aatcgctatc aaaatataga cagcgaattt gtagattatt
     3721 tggaaaaatc ctttaagctc agtaaatatt ctattttaca aacaagttta aagcagggaa
     3781 caaaattagt tctgaatatc cttatcctat ggtttggcgc tcaattagtc atgtcgagta
     3841 aaatttctat cggtcagctg attaccttta acacacttct ttcttacttt acaactccta
     3901 tggaaaatat tatcaacctc caaaccaaac tccaatctgc gaaggttgct aataaccgtt
     3961 tgaacgaagt ctatctagtc gaatctgaat ttcaagttca agaaaaccct gttcattcac
     4021 attttttgat gggcgatatt gaatttgatg acctttctta taagtatggt tttggacgag
     4081 ataccttaac agatattaat ctcacgatta aacaaggaga taaggttagc ctagttggag
     4141 ttagtggttc tggtaaaaca actttagcca aaatgattgt caatttcttt gaaccctaca
     4201 aaggacatat ttccatcaat catcaggata ttaaaaacat tgataaaaaa gtcttgcgcc
     4261 gtcatattaa ttacctacca caacaagcct atatctttaa tggctctatc ttggaaaact
     4321 taactttggg cggtaatcat atgattagcc aagaagatat tctaagagct tgtgaattag
     4381 ctgaaattcg tcaagacatt gaaagaatgc ctatgggcta tcaaactcag ctctctgatg
     4441 gagctggtct atcaggagga caaaagcaac gaatagccct cgctcgtgct cttttaacta
     4501 aatctcctgt tttaatacta gacgaagcta ctagcggtct tgatgtcttg actgagaaaa
     4561 aggttataga taatcttatg tctctaactg ataaaaccat tctctttgta gcccatcgtc
     4621 tcagtatagc cgaacgaacc aaccgtgtca ttgttcttga ccaggggaaa atcattgaag
     4681 ttggtagtca ccaagagtta atgcaggcgc aaggtttcta ccatcatcta ttcaataaat
     4741 aaggagaatg tcatgaatcc taatcttttt agaagcgtcg agttttatca gagacgttac
     4801 cataactatg cgacagtgtt aattatacct ctttcattac tatttacttt catcttgatt
     4861 ttctcccttg ttgccacaaa agaaattact gttacttccc aaggagaaat cgcccctaca
     4921 agtgtcattg cctccattca gtcaaccagt gataatccta tcctagctaa tcatttagtg
     4981 gcaaatcaag tagttgaaaa aggggactta ctcatcaaat actctgaaac aatggaagaa
     5041 agtcagaaaa ctgccttagc aactcaatta caaagacttg agaagcaaaa agaaggactt
     5101 ggaattttga aacaaagctt agaaaaagcg actgatcttt tttctggcga ggatgaattt
     5161 ggctaccata atacctttat gaattttact aaacaatccc atgatattga actgggtatc
     5221 acaaagacta acaccgaagt ttcaaatcaa gctaatcttt ccaatagcag ttcatcagct
     5281 attgaacaag aaattacaaa agttcaacaa caaattggag aatatcaaga gttgagagat
     5341 gctatcataa ataacagagc acgcttacca actggcaatc cgcaccagtc aattttgaat
     5401 cgttatcttg tagcctcaca aggacaaaca caaggaactg cagaggagcc atttttatct
     5461 caaattaatc aaagtattgc aggtcttgaa tcatctatcg caagcctcaa aattcagcaa
     5521 gctggtatcg gaagtgtagc aacttatgat aacagtttag caaccaaaat tgaagtactc
     5581 cgcactcagt ttttacagac agcctcacag caacaactaa ctgtggagaa tcaattaaca
     5641 gaattaaaag tacaactaaa tcaagccaca cagcgtttgg aaaacaatac cttaacctcc
     5701 ccaagtaaag gtatcgttca tctgaacagc gaatttgaag gtaaaaatag aattccaact
     5761 ggtacagaaa ttgctcaaat attccctgtc atcacagata caagagaagt actaatcact
     5821 tactacgtat cttctgacta tctacctcta ctagataaag gacaaactgt aagattaaaa
     5881 ctggagaaga ttggaaatca cggcaccacc atcatcggcc aacttcagac aattgatcaa
     5941 actcctacca gaacagagca aggaaatctc tttaaattaa ccgctcttgc aaaactatct
     6001 aacgaggata gtaaactcat ccaatatggc ttacaaggtc gcgtcactag tgtaactaca
     6061 aagaaaacat attttgatta tttcaaagat aaaattttaa cacattctga ttaattttca
     6121 gataacactc tataactatt tattatctta tcaaaaagga gaatcataac atggataaga
     6181 aacaaaacct aacttcattt caagaactaa caactaccga actcaaccaa attacaggtg
     6241 gaggattgtg ggaagattta ttatataaca ttaatagata tgctcattac atcacataag
     6301 aacttcatca tccaatacaa ctataaaaaa ataagaccga gaaacaagta ctctcggtct
     6361 tatttttcat cattctgtat gtatcacagt aagtacctga cgaaagactt gattttgaca
     6421 ggtggtattt agactggtat taggatggct ttccacaatc ttcatgacgg tatagagacc
     6481 aactcctctc tcctcccctt tagaactggc tccaaaggag aagatttcag aaatatcgat
     6541 gccctcttct ttgatggagt tttcgatgat aaaggtctcc tgtgctccat tttttaaaaa
     6601 ggcgattgaa acatgaggtt gactaccttc cacactggct tcaatagcat tgtcacaaag
     6661 gatagacaca atggttagaa aatcaagtag actcatcccc tcgacctgaa tctcctcagg
     6721 aacttcgaca ttaaagacaa tgttcttatc tctagctttt agaaatttcc ctgctaggag
     6781 acttttgagg gctttatcac gaatattcac caatcgacca aggtcatatt tattgtcctg
     6841 caatttttca ctagaatcct ttaagaccga gtcgtagatc tcttttatct gctccatatc
     6901 ctcctcttca atgcccagac gtaagctagt caagaggttg gtgtagtcat ggcgaaaact
     6961 ccgtacttcc ttataaagct cctctatatg ccgactatac cgctccatct ctctgtagcg
     7021 caagtcctgc tcttggttca gtctctcatg gagtttgtcc ttcaaatagg tatccaattt
     7081 cttgataatc cccataaaaa agagcaggta aaagactagg atgagatggc gaacagtcgt
     7141 tgattgaatc ccctgttcat attcaaagta agacagattt tgtatcacca aatagtaagc
     7201 ccccattatc cagttaatct gagtcaggga cttttgaaaa tctttatcga gaatcccctt
     7261 tctcaagcta gtgaaatcat agtccaacca tttcaaaaaa gctagagaaa tgaagaaatt
     7321 gaaaattatt atacataacc aaataaatga gtagtcatca tatacttgcc cttgtcccaa
     7381 aaatggaagc acaaaatagg aaacacctct ataaaagaga ttcactaata tcattggaaa
     7441 gagaccataa aagagaagga gttttttagg aagttctctt aacaataaga aagataagcc
     7501 tatgccgtac aagggttcca taaaataaga tagataagta tttcctacca tatagccaag
     7561 cattccaaaa acaacggcca acagtatctt aaaaagaaag gccttaaaaa tcctctcaaa
     7621 agtgagacta attccatcta ccttaaagaa tatgacaatt tttaatccgt gagtaacaag
     7681 tgtatacaac aatatccaag caacattcat aaactctcct agctcagtgt aagttattaa
     7741 tagcctcaga cacttccctg accttataac gcgcgattag acaacttcca ccattgggaa
     7801 agaaaagcag tttttctttc ttatctaaat gcaccacatt ggcaggattg atgagaaaag
     7861 agcgatggca ctgcaagaga cgaggctcct gcttgagaac ctcctctaga ctcgctgtaa
     7921 attccagcct gtctgtcttg gtatagagaa taacacgatg gggtcttgtg gacgtttcga
     7981 gatagtaaac ctctttaaaa ggatactgga attgagcaaa ttttgattta aagtaaaagc
     8041 aatcttccgc tagactttta ctgtcttgac cattggcata gaggagggct gtctcgatac
     8101 gagattcaaa ctcctctgcc gaaagggcct tatcaatata gtccagagca gacacttggt
     8161 agcgaaagga caggggcata aactccgagt gagtcgtcac aaagacaatc agggcatacg
     8221 gatcccgatc ccgaatcttt ctagccactt ccagaccctt catctcctca tttcgaatct
     8281 caatatccaa aaagaatagc tgatgggccc ccttctcatg cacctctgcc agcagttggt
     8341 ctggcttgcc aaatacctca aaagagctgg gagtgatatg atgttccttc aagagtttct
     8401 caatcgtcgt ttcaatccta gtctgttggg aaaaatcatc ctctaaaaca aatattctca
     8461 tcttcttact ctccctcttt caaccatttt tgacgaattt ctctatagcg acgtctggca
     8521 tacttgacag cgtattgacc ttcttctccg agaaaaagga ttctttcttg aaaatcaatc
     8581 gattttaatt tatcaagatt aaccaaacaa tttcgatgac atctcatcaa ggtttcccca
     8641 tattggttct caagattact taaattttga agcatattaa aactagcttc ttctgtaaca
     8701 atctgtacgg tatgggcttt agttggatgt gtttggatat agtagatatc gtcgacgttt
     8761 actttataga ctgttttctg cgtctgaata atcatatgtt tcattggatt ctcctcctaa
     8821 gaaaagaata acagaaaagt tctagcccaa gcccacccaa tcctaaacag tctttgaaag
     8881 ctcctaaaag gattattttt ctacaacatc atgatgagct tacaactatt ttatcataaa
     8941 attcactcag cgcaattcaa gacatttcaa tgacaattaa agatttgaat aaaaaaaaag
     9001 aattaaaaat gataaactaa cagtatcaaa acacagttgc cagaagtaag actggcaccc
     9061 agattaaagg aggcattccc atgacagata cagaccccat caaaagagct catactttga
     9121 ttactgactt aaacaaagcc taccaagcat gcaaacaggc aagcgctgac gacgtccgct
     9181 ttcaggagca attaaactct attcttggtt ttttagccaa ggctgaaaca gtggataatc
     9241 gattcttgat tgaactagaa aaattttacc agacttccag tcttctcatg ggactcagcg
     9301 ctcttgatcc agatgctcca actcgcgccg cttggcgggc ctatgaccgc ttccactttg
     9361 accaagtcaa gaccaa
//
LOCUS       DAVBCG010000077         9091 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBCG010000077 DAVBCG010000000
VERSION     DAVBCG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.077"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(2..1681)
                     /locus_tag="VQ495_001452"
     CDS             complement(2..1681)
                     /locus_tag="VQ495_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2531398.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(1683..1916)
                     /locus_tag="VQ495_001453"
     CDS             complement(1683..1916)
                     /locus_tag="VQ495_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2531399.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            2298..2519
                     /locus_tag="VQ495_001454"
     CDS             2298..2519
                     /locus_tag="VQ495_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531400.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(2732..2884)
                     /gene="cibB"
                     /locus_tag="VQ495_001455"
     CDS             complement(2732..2884)
                     /gene="cibB"
                     /locus_tag="VQ495_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2531401.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(2886..3071)
                     /gene="cibA"
                     /locus_tag="VQ495_001456"
     CDS             complement(2886..3071)
                     /gene="cibA"
                     /locus_tag="VQ495_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2531402.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            3249..3932
                     /gene="tsaB"
                     /locus_tag="VQ495_001457"
     CDS             3249..3932
                     /gene="tsaB"
                     /locus_tag="VQ495_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2531403.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            3929..4366
                     /gene="rimI"
                     /locus_tag="VQ495_001458"
     CDS             3929..4366
                     /gene="rimI"
                     /locus_tag="VQ495_001458"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2531404.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            4356..5366
                     /gene="tsaD"
                     /locus_tag="VQ495_001459"
     CDS             4356..5366
                     /gene="tsaD"
                     /locus_tag="VQ495_001459"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2531405.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(5412..6712)
                     /locus_tag="VQ495_001460"
                     /pseudo
     CDS             complement(5412..6712)
                     /locus_tag="VQ495_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6988..7339)
                     /locus_tag="VQ495_001461"
                     /pseudo
     CDS             complement(6988..7339)
                     /locus_tag="VQ495_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7446..7738)
                     /locus_tag="VQ495_001462"
                     /pseudo
     CDS             complement(7446..7738)
                     /locus_tag="VQ495_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            7981..9091
                     /locus_tag="VQ495_001463"
                     /pseudo
     CDS             7981..9091
                     /locus_tag="VQ495_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT         2633 a         1674 c         2041 g         2743 t
ORIGIN      
        1 attattcttc atctggtgtg aggatcatcg ggatgatgat cggttcacgt tcggtatttt
       61 catagaggaa ggggcgaata gcgttgacaa tggcaccatt gacagattgc acgctagcat
      121 ccttattttt cagtgcgata cgaatggcat tgaagaggat acgctggctt tggcgaatca
      181 agtctccaga ctctctcatg tagacaaagc ctcggctgag gatatctggg ccagacagaa
      241 tcatctgcga tttgaagtca acagttgcga ctgccagaac gacaccgtct tcagatagat
      301 cgcgacgatc tttgaggaca gctgcgccaa tttcaccgat acgatttcca tcgacataga
      361 tatcttgggc gttgaaatga cctgcgatac gagctgagtc agcagtaagg gcaagcacat
      421 cgccattgct catgataaag atattgtctt tctcaacacc agtatccact gctagtccag
      481 cgtggacttt ttgcatgcgg tattcaccgt ggacaggcat gaagtatttt ggcttaatca
      541 agcggagcat gagtttttgc tcttgctgac caccgtgtcc agatgtatgg atattgttca
      601 ctttaccgtg gataacttcg acaccagctt cagaaatgat gttaatcagc ttgttgacgc
      661 tagtagtgtt tccagggatg ggactagaag agaagataac ggtatcacct ggttgtaatt
      721 gtacttgacg gtgggttccg ttggcgatac gagagagggc tgccataggc tcaccctgac
      781 tacctgtaca gaggataaga acttctcctg caggataatc tttgatttca tttggctcga
      841 taaaggttcc cttaggagct ttgatgtagc caagatcgat tccgttgaca atggcctttt
      901 ccatagaacg accaaagacc gcaatcttgc gtccagtctt aacagcagct tctgttgcct
      961 gctggagacg gaagatattt gaggcaaagg atgcaaagat gatacgtcct tcaatacctt
     1021 ggataatctt cataatggac tgaccaacga ctttttcaga gttggtaaag gttggtactt
     1081 ccgcatttgt cgagtcagac aggagacaga gcacgccttc ttcaccaagc gcagccatac
     1141 gatgcaagtc cgcaggttct ccaactggag taaagtcgaa cttaaagtca cccgtacaga
     1201 cgattttccc ttgaggagta tgaatgacaa tccccaaagg ctctggaata gagtgagtcg
     1261 ttctaaagaa agttgccttg agatttttaa aggtcaactc ggtgttgtgg ttgatttcgt
     1321 aaagtttggc gttgcgcaag aggccgtgtt cttcgagttt cccacggatc aaagccaagg
     1381 caagcggtcc agcgtagata gggacatttg cttgcttgag taggaacgga atcccaccaa
     1441 tgtggtcctc gtgtccgtgt gtgattaaaa cagccttgac gcggtcgata ttgtctacga
     1501 tgtaagagta gtcaggaatg acatagtcga taccaagcaa gtcatcttct gggaatttaa
     1561 tcccagcatc gacgatgata atctcgtctt ggtattcaat tccgtaagtg tttttcccga
     1621 tttctcctag accaccgatg gcaaaaacac cgacttcttc aggtttaaga gtgtaggcca
     1681 tattagaact ccgtaatttc gaaggcgcca gtttcttttt cgtaatcgag caatttgtca
     1741 gacaagagtt cgatatattc gatattgtac tctgggcgat tttcttcgac aagttggcga
     1801 gcagtgatac ggccctcaag ttctgagctg gcatcgatgt ctaggtaaag cgcgcgtgtt
     1861 gtttcacggc gtgggctacg ttcttttgtt tcttgataaa aaactttgta aatcatatag
     1921 ttcctttcta tttacaggtc agcttattcc aaacttttag cagatatttg cttgatttca
     1981 ttccattttg tgtctagatt gaccttgatt cgttacaatt atttcaatta taccacaaaa
     2041 tgaaaaatta gtaaaagacg gaaacgagaa agttagggga cgatttcttc ggtaagcgtt
     2101 tgcaaaaaca aagaaaatgt gttatgttga taacggaagt gaagtttatt acttgtcata
     2161 attccatata gattttaagt caaggaggtt gccctatgta taacttattt gcttttgcta
     2221 tcggagttcg tttgctagct tttatcgttt ttgttttgcg tttatggagg aaatcttcta
     2281 tttgccaaac tggaacgatg acaaaccaag ttcttgagga aaattacctt tgtgactctc
     2341 tactcgcttt ttctcctcct cgtgacctat gtcgtttggt ttgtatttta ctttggatta
     2401 aatgcttaga tgccctgctt atgctggggt tttttgtttg gggatgtaga gatagtagat
     2461 ttacgacacg aaaattccac cccatctatc aagatgaggt ggtctttgca tttttctaaa
     2521 atgatatgac aatggaattt tcaaagctta ttatttttat gggagtcatt gttctgctca
     2581 aaaatagagt tgaagcataa aaataaaaca atacaaacag ataaatcgaa tggtaaaggg
     2641 atggtagtaa tgttcaaatc attgagaatg ctgagcaaac tgtaagagat gaagacaata
     2701 aaatttatcc ttctaaaaat gtgtttctta tctagcatga cttaccaaac tttttacgaa
     2761 ggtcttttcc aatggtataa cctgccataa ataaagcgat accaacacct acaccaaagc
     2821 cacccttgat ttcttgcaat tccttattag aaatttcacg acagttgttc aaatctttca
     2881 tcatattaat ctcctcctaa aaaataacca gctgccaaac cagaacctat aagtgctgtt
     2941 ccacctgcaa tagctttcca agatactgct actccaaaga taaccaaggg agcgagaccg
     3001 ccatcaatat ctgataattc attttcagat aaggataaaa attgattgtc aagaatgtca
     3061 aaatttgtca ttgaactttc ctcctttttt cttgttcact tttatattcg gaaaagaaaa
     3121 ggaaaagtga gcattttttt aaattatttt tagttaagtg agggcactct tgtctggggg
     3181 caaagaaaat tcccatgtgc catcttttgt agtataatag taagcagaca aaaagatagg
     3241 aatgtgttat gaaagtatta gcttttgata cgtccagcaa ggctctttct ctcgctattt
     3301 tagaggataa gcaggttctt gccgagacga cgattaatat caagaaaaat cacagtatta
     3361 ccctcatgcc agccattgat tttttgatgg agagtttgga ctggacgccc aaggatttgg
     3421 accgaatcgt ggtggcggaa gggccaggta gctacacagg cttgcgaatc gcggtagcca
     3481 ctgccaagac cttgactcat actctgaaca ttgagttagt tggtatgtct agcctcttgg
     3541 ctctggtgcc atatcaacaa gaaggcttgt tcgttccttt gatggatgcg cgtcgcaaca
     3601 atgtttatgc aggattttat gaaaatgcta aacctgtcat gccagaagcg cacctatctt
     3661 ttgaagaggt gctagaaaaa gtcaagggtg ctagtcaggt aacctttgtc ggagaagttg
     3721 gcccctttgt ggagcagatt caagaacact tgccaaggac taattataaa gaaaccttgc
     3781 ctaatgcagc caatcttgct cttttagctt gggacaagga agcagactcc ttgcatgatt
     3841 ttgtgccaaa ttacctcaaa cgagtcgagg ctgaggaaaa ctggctcaag aaccataccg
     3901 agtctggtga gtcctacatt aaacgcctat gatagaaatc aagcgaattc aacaacagcc
     3961 tgacctagct caagccatct acgctgttat ggcagctgtt tacctagtca gtccttggac
     4021 tctggagcaa atccaagcag atctgtccca agaccagact tggtatgcat tggcttatga
     4081 tggggcagaa gtgattggat ttctagctgt gcaggagaat ctttttgaag cagaagtcct
     4141 gcaaatcgct gtcaaaggag cttatcaggg tcagggaatt gcgtcagcct tgtttgctca
     4201 attgccgaca gataaggaga ttttcctcga agtcagaaag tcaaatcaac gagcgcaagc
     4261 attttacaag aaagaaaaaa tggcggtcat cgctgagcga aaggcctact accatgaccc
     4321 agtcgaggac gccattatca tgaagagaga aatagatgaa ggatagatat attttagcat
     4381 ttgagacatc ctgtgatgag accagtgtcg ccgtcttgaa aaacgacgat gagctcttgt
     4441 ccaatgtcat tgctagtcaa attgagagtc acaaacgttt tggtggcgta gtgcccgaag
     4501 tagccagtcg tcaccatgtc gaggtcatta cagcctgtat cgaggaggca ttggcagaag
     4561 cagggattac cgaagaggac gtgacagctg ttgcggttac ctacggacca ggcttggtcg
     4621 gagccttgct agttggtttg tcagctgcca aggcctttgc ttgggctcac ggacttccac
     4681 tgattcctgt taatcacatg gctgggcacc tcatggcagc tcagagtgtg gagcctttgg
     4741 agtttccctt gctagccctc ttggtcagcg gcggacacac agagttggtt tatgtttcgg
     4801 aggcaggaga ttataagatt gttggggaaa cccgtgatga tgcggttggt gaggcttatg
     4861 ataaggtcgg ccgtgtcatg ggcttgacct atcctgcagg tcgtgagatt gacgagctgg
     4921 ctcatcaggg gcaggatatt tatgatttcc cccgtgccat gattaaggaa gataatctgg
     4981 agttctcctt ctcaggtttg aaatctgcct ttatcaatct ttatcacaat gccgagcaaa
     5041 agggagaaag cctgtctaca gaagatttgt gtgcttcctt ccaagcagca gttatggaca
     5101 ttctcatggc aaaaaccaag aaggctttgg agaaatatcc tgttaaaacc ctggttgtgg
     5161 caggtggtgt ggcagccaat aaaggtctca gagaacgcct agcagccgaa atcacagatg
     5221 tcaaggttat catcccccct ctgcgactct gcggagacaa tgcaggtatg attgcctatg
     5281 ccagcgtcag cgagtggaac aaagaaaact tcgcaggctg ggacctcaat gccaaaccaa
     5341 gtcttgcctt tgataccatg gaataaagag tggctctttg tcaagtgtag tgggtagtcg
     5401 aaaagctgca atctggagag tacgaaattc gtactctctt ttttgatgtt caaagtcatc
     5461 aaaatccgtt ttttaaaatt gttaaagttc cggaaaccaa aggctttgcg cttgataatc
     5521 ttaatcaaat tattggtcgc ttcaagttta gcgttggaat aaggcagctt aagtgcgttg
     5581 ataatcttat ctctatccct taaaaaagtc caaaagaccg tttgaaaaag cggatgaacc
     5641 gttggcagtt cttgctcaat caatccaaag aaatggtcgg cattcttctc ttgaaaatga
     5701 aagagcagga gttgatagag ttcgtagtga acctgtaatc cctcagagta agaaagcagc
     5761 ttatctcgta cttcccgatg ggttaagtgc attcgaaacg tgggctgata gaatcgtttc
     5821 tgacagagct tgaagctata ctggtgtagt aaatcagtcc agtggactga tttagcccga
     5881 gcctagaaac gaaaaagcga gggttccaaa agcgcttgag cgcccgatac tccaaggatt
     5941 ttcggtcaaa ttggttcatg atttggattc gtactcggtt catagctcgg ctcagatgtt
     6001 ggacaatgtg gaagcggtca agaacaatct tcgcctttgg aaagagttgc ttagcgagcc
     6061 gataataagg gctgtacatg tccatggtga tgacctcgac cagctcccga acctctctct
     6121 gatagcgttg gaaatggttt cgaatcaccg catgagttcg cccgtctaaa attgcgagga
     6181 tggatttgga ctcaaaatct tgggcaatga agctcatttt gctctttttg aagctatact
     6241 catcccaact cataactttt ggcaacttgg tccaatcggt ttcaaactta aattccctca
     6301 gttttcggat gacggtggaa gttgagaccg ccaatctgtg ggcaatctca gtcatagact
     6361 gcttttcaag gaggagctga gcgatttttt gtctcaccat gttggaaatc tggcaatttt
     6421 tcttgacaat ggatgtttga gaaacgacca ccttaaggca attcttgcac tgaaagcggc
     6481 gctttttgag atgcagtacc gtgggtaaac cctgacagtc gagaagcgga attttagagg
     6541 ctttctggaa gtcgtatttg atcatcttcc cttgacaatg aggacaagga ggagcggggg
     6601 aatccaactt tgcctgaacg actagatggg tttggtattt cagaacagac aagattttga
     6661 tatttttgtc ttccaatccg agcaaatctg tggtattctt aagttgttcc atatggaccc
     6721 tttctaatga gttggttaga cgcttttcat tataggtcat atggggcttt ttttctacaa
     6781 gaaacgaccc tataattcct ggggtgggat tacccactac agaaattata gagccaaagc
     6841 attccaaagt cttgtctgac tctggaatgt tttctgttca cagttgtttt gtaactgcct
     6901 aataagtaac cgcgctataa cgaggtatct accgagattg tcaattctcc agtatactgt
     6961 tataaaaaac aaaactggag gattttttta tgtcctccca tcgtcccatc gactgttccg
     7021 tagctgccca ataacggagt atagtcggct gaaacaagaa taggacaaaa gtgcctcaag
     7081 aaaagcatcg taatttgaca atattattct gagatgtttt tgatgtaagc ccatattttc
     7141 tcaatgggat tatactcggg tgaataggta ggaagtggta acagtctatg ccctgctcct
     7201 tacacatgtt cattctgtga aaccttgcat tgtccataat gataagggat ggtgtatcta
     7261 aagtaggtag taagaatttt tggaagcaag cttcgaaaaa gtcactcgtc atcgtatctt
     7321 tataggtcat cggagccata gtatattgaa acaaaaaagg gcattagtac ttctataaca
     7381 gtatcgcaat ctctggtgtg cgttgtctcg atcttaattc aaataattat atcttcttcc
     7441 agagagacta actgcctgct caaggctcga tcatatttga gataaaaata ggtctcaacc
     7501 cctgtctcat aaatataaat aggagtcagg tagcttaagt gattcaattc tttaaggaac
     7561 cgatttactt tttctgggtc ttgttcgcag taggtacggc tctttttaga ctatatccca
     7621 tagcctttag agcgtaatga atagttgttg gaggacagtt gaattcagca gctatttcag
     7681 tcaaataagc atcgggatga gtttcaatat agatctttaa tttatctcta tcaacttttt
     7741 caattaatag tggaagtttt cttaaagtta gtgtgttgaa acgtttttat acagtgctaa
     7801 aatttgaagt gtagtgcaga tgtatttgta atagtattat agcaattctt tggagaaggt
     7861 cgagacaaga gaaaataggt atctttgaga tataagtatg atttactata aaattattga
     7921 gttattcata aaaatttctc tatcaaatat cataatgcgt cgtttaagga gatggttaca
     7981 atgcaatata gttgtggaaa aataaatatt aatatccctg atggatatgg agacattaag
     8041 gatatagtgt tttcggctca tattatagtt aggtataata atggacattg tggaggtata
     8101 gatccgcata taattggact ttgtaaaaag cagatcagaa ggatgtctcc atatcctatt
     8161 ttgataatcg tatctagaga ttcaaaggtt attgatgatt ataaaaattt agatattgcc
     8221 tatgttgatt gtactcaatg ttcaaataat tttgaaactg ccttacacgt taaaaatatt
     8281 ttaaaattat taaaaatcca acttatacac tgtcatggct actcaactaa ttatttttta
     8341 tatatgttaa aaaaacttga caagaatgga tttggaaagg ttaagacagt gataacatgc
     8401 catggatggg ttgaatataa cttaaaaaag aaattcttga cttattttga tttttggaca
     8461 tattctatgg gagatgcttt tatttgtgta tctgaaacta tgaaaaaaag attggagagt
     8521 ataataaaaa aataagaaga tagttgcaat aaataacgga attaatgtat caaatagcga
     8581 tttagatgta gtaggtgttc aagactttaa aaaagaattt tgtattccaa ataacaaaaa
     8641 aatcatttgt tatgttggaa ggttggatcc agaaaaaagg ccggatagat tccttgaatt
     8701 tgcagaaaaa ttatttttag tcagagaaga tgtgattttt attatggctg gaaatggtag
     8761 tatgtgggct actttgaaag aaaaaatttg ccatttgaaa tgtagagata atttcaggtt
     8821 acttggtgaa atctatccag ctactatagt ttaccaaata tcggatttat tgtatattcc
     8881 ttctgataca gaaggaatac ctatgtgtgt tttagagtca atgtcacaag aaactccggt
     8941 tttagctagt aatgttggcg ggttaagtga aattattgaa catagggttg atggattttt
     9001 atttgagaag gaagatgttg agggagtgtg tgcttgtgct aattttttac tcaatgattc
     9061 tgagtatttg aaatatatag gtgagaatag t
//
LOCUS       DAVBCG010000078         8964 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBCG010000078 DAVBCG010000000
VERSION     DAVBCG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.078"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            169..396
                     /locus_tag="VQ495_001464"
     CDS             169..396
                     /locus_tag="VQ495_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531406.1"
                     /translation="MATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDGYNKINLI
                     NSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            553..1611
                     /locus_tag="VQ495_001465"
     CDS             553..1611
                     /locus_tag="VQ495_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531407.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFDGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            1926..2900
                     /locus_tag="VQ495_001466"
     CDS             1926..2900
                     /locus_tag="VQ495_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531408.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSTK"
     gene            3280..3654
                     /locus_tag="VQ495_001467"
     CDS             3280..3654
                     /locus_tag="VQ495_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2531409.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            3862..4764
                     /locus_tag="VQ495_001468"
     CDS             3862..4764
                     /locus_tag="VQ495_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531410.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            4820..5059
                     /locus_tag="VQ495_001469"
     CDS             4820..5059
                     /locus_tag="VQ495_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2531411.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINENPYPWDGFISATVR"
     gene            5056..5784
                     /locus_tag="VQ495_001470"
     CDS             5056..5784
                     /locus_tag="VQ495_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531412.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGKVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            6919..7158
                     /locus_tag="VQ495_001471"
     CDS             6919..7158
                     /locus_tag="VQ495_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531413.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            7708..>8964
                     /locus_tag="VQ495_001472"
     CDS             7708..>8964
                     /locus_tag="VQ495_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2531414.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPA
                     PQPGWKQENGMWYFYNTDGSMATGWLQ"
BASE COUNT         3089 a         1214 c         1655 g         3006 t
ORIGIN      
        1 ggtgttggtt gtaactggtg gaacaaacgt gataatattg ctgcagctgc agtttctgga
       61 gggatgtcag tataccagac atatgccatt aaaaagttaa ttgctaatcg tggtatgatg
      121 agccgagtcg ttagaagtgc tataacaaga tatattggtg gagttagtat ggcaactatt
      181 actaatgctc ttaatattgc ggctactgtt gctgaagttt tctcattagg aggagccatt
      241 gcatatggtt tagatatcgt agacggtaaa tttgatggat ataataaaat aaatctaata
      301 aattctatta ttatagctct tggtatgata ttggcattaa ttttggtatt atcaagagtg
      361 tatagaaaat atttttatag agacgatgat gactaaagtt tttatcaata atttgggctc
      421 cttgtcaact gtagtggatt gaagaaaagc taacgtctgg ggaagggttt aaagctttgc
      481 tttatcaaat attgacttac agagttgctc tattgtattg atgtattctt agtaaataat
      541 atggtgtttt gaatgaaaga tgatcagaaa tatttattag cgggacttta ttcgcttcta
      601 gttgcaatat tttattttcc attgattgag tccaagggga tttttgtaag tatattgatg
      661 gcggttttac ttttatattt aatatacttc attgcaacag tcattcatat tgtgataatt
      721 aagtttataa gaaaaaaatc ttttaagtat ttggttctat atccatttac ttatgatggg
      781 agctggagat ttcaacctat aaacttactc tactttccag aaatggtgag agatgtcatt
      841 ccgattaatt tagttcaaga atattgccaa ggacaacctt acggactgtt aaaaaagatg
      901 ctgaaaagaa ttcgattgtc acgagagata gctttactcc tggctaccat tatcgtctat
      961 ttttttacgc atagaattct acctctttca gtatttacgt tcatattttc ttacatattg
     1021 ctatttgcac aatcttattt gggtggtaat accgtttgga ttggaaatag aagattaata
     1081 atagatgatg aatttgaaaa aatcttgctt tcaaaaagtt atataaaaga aatatcatct
     1141 gcacgatact cggaatactt gacatgtgaa tacaaaaacc ttacaccaat catattgtta
     1201 gctatttttg aaaatttgct tgactcctac ttgcttcaga atcagagtga ggttgactta
     1261 gatatttttt ataaagtttt acctttgctt tataaagaaa aatatactat gggattcaat
     1321 tactttgtaa gtttaaatta tttactatat aaggttggat ttttgggaat catttacgat
     1381 aatgaagcat taggagattt gtcaaaacaa tatttaaaca gaaatatttc ggaattacaa
     1441 gatggttctt tcgatggcgg tatacaagat gctgttgcct ctaaacaaat agtagttata
     1501 aatgaattta tagcttgttt aaattccaga tgcatgccat cccaatatga tagatttttt
     1561 tataaagata gaccttatat tttttccaga aagagtccta ttaaggggtg aaaatttgtt
     1621 acatttggat taacaagtgt cctagggatt agaaataact ctagtatagc tagatatttt
     1681 tgagtaacta gaaatatcat taggctaccc atatgattgc cagaaaagat ggctgatgaa
     1741 tcttatttat actgttgcag taggcattgt ctaggataaa taagctatct tgatggggga
     1801 aattggaata attttagtag aaacattaaa ggattatgga caagtagcgc aggattaggt
     1861 gatgcaatgt gctaagttgg cttggtgtta gcagtagtat tatttaacaa tggaaagtag
     1921 actaaatgaa aaataagcgt tatttttttg acacaatttt aatgatttta ctgcttataa
     1981 gcacgatatt ttgtgtcagc ccagtattta taaaattgga catcttagga accccctctc
     2041 atgccatctt aacctttgta ttggcgatac cgttattcta tattttgtct caatgcttac
     2101 atactttgct attactagtt tcctcaatct tttgtaaatt aagaccaatt tatttttatt
     2161 ttatatttgt gatcattata ggagctagga aatactatcg aattctcttt caccaactaa
     2221 tgggatttag tccaggaata gcagtgtttt ataaggagag tcaaacaact aagaatctgt
     2281 ttaaatttta ctacttcttg tactttacaa cactaatttc atactatttt ttctttactt
     2341 ttgtgtatga caaaccgttg cttttgcctc ttattccatt ttctattatc atagcattag
     2401 tacaaaaact ttatagaatt gagaatcaac aattatttct gttaaaatca aaggtactaa
     2461 caatactgga atctaagaag aattgtgagt ttaatcttca ggattatcat gaaatttgga
     2521 aattgcaatc aaaatctgag ttgccatgcg tggctttatc ttatatcagt ttgatcaagc
     2581 catatctttc agagagcgtg cgtgaacaaa ttgatttatt agaagttaag cgatttaaaa
     2641 aaataaatca tccaatatca ctttatggta tgttggatgt tataaaatta aatctatatt
     2701 tacggcatta taatgaaaaa aataaatatg aatcaatgtt aaagaaaatt ttagaggtaa
     2761 gaccagactt tgttttaatc gagcagaata ttgatgatag tttaaatagc tcgcaaccat
     2821 tatctttatc tttagccatt tcagaaatcc aacttttgct tgaagtgtat atggggatca
     2881 aacatgtttc aaccaagtga taaaaccaga ataatatttg ccattatagg ggcaagttgc
     2941 atattagtca gtatgtatct tattccactt attaaaactg agaataaatt ttgggaaata
     3001 cttttattta tattaggtgt attgctaatt ggaattgcgt tactaagtca aaaaaaataa
     3061 attttgaaat agtattagtg agttcgcaat catttattgc tagtggttta gaactgttaa
     3121 gtttcaaaca ataattgatt ttaaacctta ggtaacctat aaaaacaaat aaggtagaga
     3181 aaggatatct tatctaggta gctaagtgat aaaaacacag tcgtctatca cttgttagga
     3241 aactatccta actatgctta ataaaaaagg agaaaataaa tggaactcgt attaccaaat
     3301 aattatgttg ttcttgagca agaagagatg atgtatcttg atgggggatt ttctattccg
     3361 agatggcctg ttgcaacagc cattaatata gcttttaatg gtgttttagg tggaggagca
     3421 atcagtctag ttagaaatta tattcgtaat tatggtttgc ggcgagttac aagcgcaatt
     3481 gctggagcag ctgcaagata tgttggggta cgagttgcaa atagagtggc aggatttgca
     3541 ctgtctgcta ttaatggatt tgcagcttgg atgtcaattg gcgatgctat tacaacaatc
     3601 tgggccaaca atgatgtaaa taggagagac ccaaatttaa acgccttgtg gtaactagat
     3661 atgggtatta aaatggaaaa aatttttgtt attatttttt ttgtatgtct atttatatca
     3721 tccataactt ttttagccta tgattttgtt agcgaagaaa taaaaaagtt gattatttgg
     3781 attaatgtcg tttttttgat tttaatcata gcaatgatga tttatccaaa attaagaaaa
     3841 tgataaggac actgtaaaat tatgatacgt aaactaattg ttttcttact aatgctccct
     3901 ctttttgtct tgtggctaga gttgcatgtc ctagtgaata acttactatt gaatctagaa
     3961 tctccccttg attttgtgat tagtatgagt cttgctttct gtagtttgat tttatcaaaa
     4021 atagttttag acatactata cgcattgaaa gatttataca agaaagaagg tcttattaag
     4081 atttttccct ttatttttat aagaaggaaa aaagtgaatg tgagattctc gccctatttt
     4141 tcattccatc gtaaaagttt gtcgcctgat gatttaagat ctaggattat atggagtttt
     4201 attcttgaaa ttgctattat cctagtattt attttaaaaa ttccttttgc aatcatcatg
     4261 cttactacaa tatttttctg gactataatg gatatcaatc atctcgtttt taataagacg
     4321 gaatttcttt ttaatcaaaa caaatggcaa aaagaagatt catttgaaag taatttgcat
     4381 aagatattga tggacaaaat acaaaaatca gaattaagct attctgactt aatgtccctt
     4441 cttctctatg atgcgatgaa ccaatccacc tttttaacgg atagtgaact ttttgaggat
     4501 attttgaaaa aaatcgaaga ttctcataac acccttctgt gtacaggtct tgtcgagtta
     4561 ctgctgtatg agatttctat tagtaacaat aacaactggc aagaaaaggt tgataaaatt
     4621 agaatacagc tcatcagaat caatcaatta gattttttct attatacaag ttggttaagg
     4681 caaaattttg atttctgtat gaatagagag tatcataaaa tgaaatctcg gaaattatta
     4741 ctgagtaata aaaagatagt ttaagttcgt cctatttgta aaacaaatga aacaatttca
     4801 actaaggagg agaaaacaaa tggaacttgt attaccaaat aattatgttg tgattgatga
     4861 agaagtaatg atgtatcttg atgggggagc ttatttaagc aagcgtgctt gtcaaggaat
     4921 ttgcgcagca gcaggtgtat tggctacagc agcagggaaa atagcttact gtattggata
     4981 tggtgttctt aatagaggtt gtgatattaa cgagaaccct tatccttggg atggattcat
     5041 atctgcaaca gtaagatgag taatgtagat aagattcgaa aaatacacat catcgtgtgt
     5101 tgggtgtata tatttttatc attcagggcg attataaatg atacggagta ttttttgctt
     5161 atttttttag cttttatcta ctcaatagta tcactacctc tatattctgt gaaaaataaa
     5221 atagtatcta tctgtctagt tataaattcg attctgttaa tgagtttccc cattttaatc
     5281 aataagtttt tcccagaaag ttttttgact tataccgtat taataagtgt ttttatccta
     5341 gagttagcaa tctttcattt aattaggaaa gattttgata ttaaattgac taacgaatat
     5401 aagaaaatta gtcagtttag aagcaaggtg tctcaatctc cttggataaa atatttagag
     5461 atttctagtt ttatattaac tatatttcca tctattcttt atggtaaagt tgataatcat
     5521 gtactgactc ttatcttttt gataaaaatt tgtgtagata ctacgataaa atttttattc
     5581 atcagattat ttgacacaag tactttaatg aagaggagaa tattttttct tttcgcattg
     5641 gatgttatag cttacttatt tttaggatat cttttagtga ttcaaaaagc aggctatttg
     5701 ttttcggttt tacttctttt ttctaatttt tcagttccat ttatcaaaga aaaggaatac
     5761 gaattattta aaaatagcaa gtaatagaaa tatggattaa atcttagaac ggcttaaaat
     5821 cagatatgat aaagggaaat agtcttatct aggtaactaa gtgctacaaa cacaatcgag
     5881 gatcacttgt taggattaca ttttaatcat gcttaataag aatggagaat tcctatgggg
     5941 attgtattac tctgaagttt cgaatacctt agttctcgtg attttgagct attctagctt
     6001 tagaatcctt cagaacgtaa aattttaagc agtcaaagac ttagaatagc ctgaaaacat
     6061 ttattttata gcaattctag gcttagaatt gctcatatat atgtatttga gctgctcgat
     6121 agttaggaca gctctattct gagaaatctg agctatcaat gcttcagaat agtatttcgc
     6181 caatggtttt tattgaagag gtgattgtgt tatgatagtc taaaataaaa ttgatttaat
     6241 agagtaaatg gagaatcgtt acatgaaaca tttttttgca ggaattggtg agataaaact
     6301 tgttggctac ttattgtata tatgtgtagg actagcccct ctttttcata tatatgtcat
     6361 aggttcggaa ctgagttttg tgaaaatagt gttatctatt ttgggagtta tatttgtaag
     6421 tatgttaact attgctcgaa tttatcggac ttttctataa agaatagttt ggacttaaac
     6481 tgtatctaaa aacagaggtt ggtcgattat cagcctcttt cttgtcgttg aaaagagtga
     6541 gatatattga cttttgaaaa aaagatgtca taattgagtt gggataaacc taaaagtaat
     6601 atagtagatt gaatctggaa tagtccgtta tgactgttaa aatatttcta gaaattaatt
     6661 tgactttcct aatcgatttg ttcacatctt gtttcaatct actatacttt ctcgttaaac
     6721 cgttcggcaa ttcatggtga catggacaca tagtagtaag cacactatgt ggcttggcag
     6781 agctaaaaac tgttcccttg cgataagcct aataagcaca acatagggag ttagagaagc
     6841 cgactctaat caaccacttt gggcaatagt gagaactgcc ccgtgctttt ttattttgag
     6901 aaaatatgga gtttgtcgtt gaaattactt gattgtattt tggactatca agaaagattc
     6961 aatggaaaaa catgtcaagt atcaacgaat tataagtatt tagagatttt caaagtgaat
     7021 ttttgtttga ctgatttaca tcatttattt gacttataca aaatcacacg agattatgct
     7081 agtcaaacaa aacctgctat tcaagatggt gtttttattt tagaagattt tagaaatatc
     7141 ctatgtacaa tgatgtaatc gaaaggatat ctttatgtga gtttatcggc gatatcttct
     7201 atatctaagc tagcaagttg ttgcatcgta gctaaggatt tatctaaaaa tactatgaaa
     7261 ttggacgtca tattttttga ggataaaaat aaaagatccg cagttttagg tttacgaaga
     7321 gacaaaagcg gagtatttaa accagttact ctacatttta caagcgctaa gaaatatgct
     7381 aaagttcgta aaacagatgt gaaagaaatg aaatggctat aaagattgtc cgcaggctta
     7441 agcttgcgtt ttttgttgtt taacgaaatt aaaaaacaag ctctctcatc ggaaatgttt
     7501 ttataggaat gaaggaagat gatgctatgt ttaaaaagag aatcgaaaag ggcaaaagta
     7561 gtgtctttat ctttttggag tagaagctta tgatatagaa atttgtaata aaaatgtaat
     7621 ataaaacatt tgacaaatat ttacgggggg agtatactta atataagtat agtctaaaaa
     7681 tgactatcag aaaagaggta aatttagatg aataagaaaa aaatgatttt aacaagtcta
     7741 gccagcgtcg ctatcttagg ggctggtttt gttacgtctc aacctacttt tgtaagagca
     7801 gaagaagctc ccgtagctag tcagtctaaa gctgagaaag actatgatgc agcagtgaaa
     7861 aaatatgaag ctgctaagaa ggaatatgag gacggaaaag ctgctcagaa aaagtatgaa
     7921 gatgatcaga agaaaactga ggagaaagcg gaagaagaaa gaaaagcttc tgaagaagaa
     7981 caagctgcaa atctgaaata tcaacaagag ttggttaaat atatacgtga aaatgatcca
     8041 acaaaaaaag ctgaagctaa gaaagcaatg gatgaggctg agaaagagta taagaaaaaa
     8101 caaacggaat ttgctgaagt tcgtgcaaag gtaattccta gcgcggaaga attaaaaaag
     8161 actagacaaa aagcagaaga ggctaaagca aaagaagcag aacttactaa aaaagtagaa
     8221 gaagctgaga aaaaagttac tgaagccaaa caaaaagtgg atgcagaaca tgctgaagaa
     8281 gtcgctcctc aagctaaaat cgctgaattg gaacatgaag ttcagaaact agaaaaagct
     8341 ctcaaagaga ttgatgagtc tgattcagaa gattatgtta aagaaggtct ccgtgctcct
     8401 cttcaatttg aattggatgt taagcaagct aaactatcaa aacttgaaga gttgagtgat
     8461 aagattgatg agttagacgc tgaaattgca aaacttgaaa aagatgtaga agatttcaaa
     8521 aactcagacg gtgagcaagc tggacaatac cttgctgcag ctgaagaaga cttagttgct
     8581 aaaaaagctg aattagaaaa aactgaagct gaccttaaga aagcagttaa tgagccagaa
     8641 aaaccagctg aagaaactcc agctccagca ccaaaaccag agcaaccagc tgaacaacca
     8701 aaaccagcgc cggctcctca accagaaaaa ccagctgaag agcctgagaa tccagctcca
     8761 gcaccacaac cagagaagtc agcagatcaa caagctgaag aagactatgc tcgtagatca
     8821 gaagaagaat ataatcgctt gacccaacag caaccgccaa aagcagaaaa accagctcct
     8881 gcaccacaac caggttggaa acaagaaaac ggtatgtggt acttctacaa tactgatggt
     8941 tcaatggcta caggttggct acaa
//
LOCUS       DAVBCG010000079         8958 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBCG010000079 DAVBCG010000000
VERSION     DAVBCG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.079"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            180..1025
                     /locus_tag="VQ495_001473"
     CDS             180..1025
                     /locus_tag="VQ495_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2531415.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            1015..2118
                     /locus_tag="VQ495_001474"
     CDS             1015..2118
                     /locus_tag="VQ495_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2531416.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            2120..3106
                     /locus_tag="VQ495_001475"
     CDS             2120..3106
                     /locus_tag="VQ495_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2531417.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            3122..4555
                     /locus_tag="VQ495_001476"
     CDS             3122..4555
                     /locus_tag="VQ495_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2531418.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            4584..5618
                     /locus_tag="VQ495_001477"
     CDS             4584..5618
                     /locus_tag="VQ495_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2531419.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            5818..6555
                     /gene="mecA"
                     /locus_tag="VQ495_001478"
     CDS             5818..6555
                     /gene="mecA"
                     /locus_tag="VQ495_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2531420.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            6706..7992
                     /locus_tag="VQ495_001479"
     CDS             6706..7992
                     /locus_tag="VQ495_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2531421.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            7994..8863
                     /locus_tag="VQ495_001480"
     CDS             7994..8863
                     /locus_tag="VQ495_001480"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2531422.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
BASE COUNT         2752 a         1534 c         1905 g         2767 t
ORIGIN      
        1 acctatacct acggcaaggt gaagctgacg tggtttgaag agattttcga agagtataag
       61 acagaaaaat caaagtttga tagctatttg gatagcagtc aggctttgat tttttacagt
      121 ctgcaataga ttttcacccc ttgtttgtgg tataatgaaa cgatacgata gaaagaataa
      181 tgaacaatat gactgattta aaagcaattc aggctcgtag tctggagatg gctgaatatt
      241 ttgtggcctt ttgtaaagaa catgatttgc tctgttatct ctgtggcgga ggcgctattg
      301 gtgcccttcg aaacaaggga tttattcctt gggacgacga cctagacttt tttatgcctc
      361 gtaaagatta tgagaaatta gcagaattat ggcctcgtta tgcagatgaa cgttatttct
      421 tgtcaaagag tcacaaggat tttgttgatc gtaatctttt tattaccatt cgtgacaagg
      481 aaaccacctg tatcaagcct tatcagcagg atttagattt gccacatggt ctgaccttgg
      541 atgttttgcc tttggattat tatccgaaaa atccagctga gcggaaaaaa caggttcgtt
      601 gggccttgat ttattcactc ttttgtgcgc aaactattcc agaaaagcat ggtgctctca
      661 tgaaatgggg aagtcgcatt ttactgggtt tgactccaaa atctctccgt tatcgcatct
      721 ggaaaaaagc tgagaaagaa atgactaagt atgatttggc tgattgtgat ggcattacag
      781 aattatgctc aggtcctggc tacatgagaa acaagtaccc aatcacatct tttgaagaca
      841 atcttttctt gccatttgaa ggaacagaga tgcctattcc aatcggctat gatgtctatc
      901 tcagaactgc ttttggggat tatatgacgc ctccaccagc agacaagcag gtaccgcatc
      961 atgatgctgt catcgctgat atggataagt cttatacaga atacaaggga gaatatggtg
     1021 gctaagaaaa aaatcttatt ttttatgtgg tctttttctc ttggaggtgg tgcagagaag
     1081 attctatcaa ccattgtttc aaatctggat ctagaaaagt atgatattga tattcttgaa
     1141 atggagcact ttgacaaggg atatgaatct gttccaaagc atgtacgcat tttaaaatcc
     1201 cttcaagatt atcgccaaac cagatggtta cgagcttttt tgtggagaat gagaatttat
     1261 tttccaagac tgactcgtcg tttgcttgta aaagatgatt atgatgttga agtttctttt
     1321 accattatga atccaccact gttgttctct aaaagaagag aagtcaagaa gatatcttgg
     1381 attcatggaa gtattgaaga acttcttaag gatagctcta aaagagaatc acatagaagc
     1441 cagttggatg ctgcgaatac aattgtaggg atttcaaaaa agaccagcaa ttctatcaag
     1501 gaagtttatc cagattatgc ttctaaatta cagataatct acaatggata tgattttcag
     1561 actattctag aaaaatctca agagaagatc gatatcgaga ttgctcctca aagtatctgt
     1621 actatcggac ggattgagga aaataagggt tctgaccgtg tagtggaagt gatacgatta
     1681 ttacaccaag agggaaaaaa ctatcatctc tattttatcg gggctggtga tatggaagag
     1741 gaactgaaaa aacgagtcaa agagtatggg attgaggact atgtacattt ccttggttat
     1801 caaaaaaatc cttatcagta tttatctcag acgaaagttc tcttgtctat gtctaaacaa
     1861 gaaggttttc ctggagtgta tgtggaggcc ttgagtctgg gactcccttt tatctctacg
     1921 gacgttggag gggctgagga attatcccaa gaaggacgat ttggacaaat cattgagagc
     1981 aatcaagagg cagctcaggc gattactaat tacatgactt ctgcctcaaa ctttgatgtc
     2041 gatgaggcta gccaattcat tcaacaattt acaattacaa aacaaatcga acaagtagaa
     2101 aaactattag aggagtagca tggaaactgc attaattagt gtgattgtgc cagtctataa
     2161 tgtggcgcag tacctagaaa aatcgatagc ttccattcag aagcagatct atcaaaatct
     2221 ggaaattatt cttgttgatg atggtgcaac agatgaaagt ggtcgcttgt gtgactcaat
     2281 cgctgaacaa gatgacaggg tgtcagtgct tcataaaaag aacgaaggat tgtcgcaagc
     2341 acgaaatgat gggatgaagc aggctcacgg ggattatctg atttttattg actcagatga
     2401 ttatatccat ccagaaatga ttcagagctt atatgagcaa ttagttcaag aagatgcgga
     2461 tgtttcaagc tgtggtgtca tgaatgtcta tgctaatgat gaaagcccac agtcagccaa
     2521 tcaggatgac tattttgtct gtgattctca aacatttcta aaggaatacc tcataggtga
     2581 aaaaatacct gggacgattt gcaataagct aatcaagaga cagattgcaa ctgccctatc
     2641 ctttcctaag gggttgattt acgaagatgc ctattaccat tttgatttaa tcaagttggc
     2701 caagaagtat gtggttaata ctaaacccta ttattactat ttccatagag gggatagtat
     2761 tacgaccaaa ccctatgcag agaaggattt agcctatatt gatatctacc aaaagtttta
     2821 taatgaagtt gtgaaaaact atcctgactt gaaagaggtc gcttttttca gattggccta
     2881 tgcccacttc tttattctgg ataagatgtt gctagatgat cagtataaac agtttgaagc
     2941 ctattctcag attcatcgtt ttttaaaagg ccatgccttt gctatttcta ggaatccaat
     3001 tttccgtaag gggagaagaa ttagtgcttt ggccctattc ataaatattt ccttatatcg
     3061 attcttatta ctgaaaaata ttgaaaaatc taaaaaatta cattagaacg ggggtgacca
     3121 agtgattgat gggaaacgat tattatttag tttgaccata gtcagttatg ccttgacgct
     3181 agtaagtgga attgtgtatc tgtttaataa taataatgtt agcttacttt ctactttatt
     3241 gttcttgttg gttagtagct taattgcttg ttggaatgat atcaagtatt acttaatcca
     3301 ttttattttc tatttaacca tttttgtatt tctggtatca agaccgacca ttgattattt
     3361 tagggatggt gctttggata cctatcatcc aatagcctat cgttttgcct ttatagttgt
     3421 catggtttcg attctgggct tgaccacagg aggcattctg gctcgttact tcatagctag
     3481 gaagaaaata aaagtagcaa atataggaaa ttctctaaaa gaggtttata tcaagcggtt
     3541 acgctttgta tcactaggag tttttcttct aacttatcct ttctatttca ttcggttatt
     3601 tgaacggctc ttgtatcgtt tgcagacttc ctactatgcc tactatgcaa attttgaaag
     3661 taaactgcct tattttacct acattttgtc tacctttacg gtctatgcaa tgtgtatgta
     3721 tctggcaacc aagccaaaga aattgcaggc cacagcagtg cttgtctcct ttattgcagc
     3781 taatactatt catttggcaa ttgggacacg aaatcccttt attttaagta ttttatttgc
     3841 ttttgtttat tactttatgc gggagcaaac tgaaaaagga aaatggattg ggtttaaaga
     3901 aaagttagcg atttttgtag gttctcctat tctcatgtta gcgatgggag tactcaatta
     3961 tgtacgggat aatgtccaag tttcccatac aggtttctgg gatatcttac ttgactttat
     4021 ctataaacaa gggactagtt ttggtgttct ggctcgaggt tttctattta acagtagcct
     4081 cccttaccga gatttccgta attttacttt tggtcctgtt cttgattatt ttgcaagggg
     4141 gagtttggga gccattttcg gaggaaaagc ctttgaacat acaaccaata gtgtggaact
     4201 agctattgat agtaatagtt atgcccacaa tctatcctat cttgtcttga acaaggaata
     4261 cctgaaaggg catggtatcg gaagtagtta tatcatggag ttgtataccg actatggtat
     4321 gattggagtc tttctgctta gtttcttact cggcgtatta tttatagcca tgctgcaagt
     4381 agcctatcgc tcaaggacaa tcctatttgc tttatcccta ctcatcttga ataatctatt
     4441 ctttatgcca agaagcagct tttcagaaag tttcttcaat ttatttacaa tgcaattctg
     4501 gggaattgtt cttgtgatta tatttatagc aaaaatgctt acaaaggaaa actagtatct
     4561 actaaacaaa ggagaaaaaa atcatgtttg aacattattc agtagctgat ttgtttgcaa
     4621 atctttataa aaagcgaaaa gcaaatattt tagctctcat cgctttattt gctctcattg
     4681 ctgtaccatt tacaattaaa gcagttagga ataaaaacac tgtcaaagat acaacaagtt
     4741 attcaactta tcttatctat aaaatcactc ctccaaaaga gtcggccaaa acgattttga
     4801 atcatcaaat tggtggttat agtgattttt atgggaaatt gattgatggt aatttgaatg
     4861 gagcttatct tttcaatgat gtagaaccca gtgagttgaa aaaaattgcc agtgaattag
     4921 acacgacaga aacaaccttg aaaaattcaa cgaatgacta ttggtggaaa aaattgaccg
     4981 tctactatat gattgacgat gcaggggttg gtgtgaaaat tttgacatca agtaaagatg
     5041 ccaatgactt gttagagaaa aaaattgatg ggttgattga gaaatttaaa catgcctatg
     5101 caaatgtgaa aattgaaaaa ctggaaacca tcaactctaa agaattgaac gcaaatggtg
     5161 aaacagcgct tggcttaaat gtgaaaaatc tgattcttcg tttagttgtt attggagtgg
     5221 tttgtgtgat tttggttgtg atgggaaatg tgttagttta tctctttaat ccaacaatca
     5281 atagagtagg tgatttttct cagtatcaaa ttgattttgt aacagagatt acaacaattg
     5341 ctaacctagc agatgttttg tcatacaaaa atactggaca ggaattgacc atcgttagct
     5401 caaataaagc tatcctagat aaattgaaac agagtcaaga agctttaaaa ggaatgcatt
     5461 ttgtagattt acaggatgta tcatctcttt tggaaagaga tacagtcctt cttgttgaag
     5521 agtacggagt gactcgttat aagaaatttg agcaaagtct tcaaattctc agaaacttaa
     5581 atcgttctat cctcggtgta gcaaccttta aattataagt attctgatgt ataaggtctt
     5641 caaaatcggc tcttagccga ttttgaaggc tttttctatc tgtctgcaga aattttctct
     5701 tggagtctcc tatagaaaga ccctagggaa aatctatccg tatagaggag ccttcttttg
     5761 ataaaatcgt aaaaatctag tataatagat agaactgaaa gtatgaggtt actagcaatg
     5821 aaaatgaaac aaattagtga tacaacttta aaaatcacga tgtctttaga ggatttgatg
     5881 gatcgtggaa tggagattgc tgactttctc gttcctcaag aaaaaacaga agagttcttt
     5941 tatgctatct tggatgagct agagatgcct gatagctttc tggatacagg catgttgagc
     6001 ttccgtgtga ctccaaaacc tgataaggta gatgtctttg tgaccaagtc aaagattgac
     6061 caaaatctag attttgaaga cttatcggat ttgccagata tggaagaatt ggctcaaatg
     6121 tctccagatg aatttatcaa aaccctggaa aaaagcatcg cagacaaaac caaggatgat
     6181 atcgaagcca ttcaatctct tgagcaagtt gaagccaagg aagaagagca agagcaggct
     6241 gaacaagaag ctgagagtaa gaaagaacct tacatctact acatcctttc ttttgctaag
     6301 ttggctgact tggtagcttt tgccaagaca gtgacttttg aaatggaaac ttctgaactc
     6361 tacaaaatga acgagcgcta ttatttgacc attttagtgg atattgaaaa tcatccaagc
     6421 ccatatccag cttggctgtt ggcccgtatg cgcgagtttg cagacgatag tgatatcagt
     6481 cgctcagtct tacaagagta tggtcaagtc ttgatgagtc acgatgcagt gctcaatctg
     6541 caaaaaatcg gctaatcttt tctggaaagc tattttcaga ttttaagaag agcgaatcgt
     6601 ctgattcgtt ttttcttttt atactgaaat agtgatttac tataatagga attttcacaa
     6661 aattctgtta taatggctat attagaaaat ttcgaggaga taaagatgac agttaaaatt
     6721 gctttactag gatttggtac cgttgcaagt ggtgtgcctt tcctcctaaa ggaaaatgga
     6781 ggaaaaatca atcaatcagc acattcagat atcaaagttg ctaaggtatt ggtcaaggat
     6841 gaagatgaaa aaaatcgctt gcttgcagca gggaatgact ttaactttgt aaccaatgtg
     6901 gatgatattt tatcagacca ggatattact atcgtagtgg aattgatggg gcgtattgag
     6961 cctgctaaaa cctttatcac tcgtgccttg gaagctggaa aacacgttgt tactgctaac
     7021 aaggaccttt tagctgtcca tggcgcagaa ttgctagaaa tcgctcaagc taacaaggta
     7081 gcactttact acgaagcagc agttgctggt gggattccaa ttcttcgtac tttagcaaat
     7141 tccttggctt ctgataaaat tacgcgcgtg cttggagtag tcaacggaac ttccaacttc
     7201 atggtgacca agatggtgga agaaggctgg tcttacgatg atgctcttgc ggaagcacaa
     7261 cgtctaggat ttgcagaaag cgatccgacg aatgacgtag atgggattga tgcagcctac
     7321 aagatggtta ttttgagcca atttgccttt ggcatgaaga ttgcctttga tgatgtagcc
     7381 cacaagggaa tccgcaatat cacaccagaa gacgtagctg tagctcaaga gcttggttac
     7441 gtagtgaaat tggttggttc tattgaggaa acttcttcag gtattgctgc agaagtgact
     7501 ccaaccttcc tacctaaagc gcacccactt gctagtgtga atggcgtaat gaacgctgtc
     7561 tttgtagaat ctatcggtat tggtgagtct atgtactacg gaccaggtgc gggtcaaaaa
     7621 ccaactgcaa caagtgttgt agctgatatt gtccgtatcg ttcgtcgttt gaatgatggt
     7681 actattggca aagacttcaa cgaatatagc cgtgacttgg tcttggcaaa tcctgaagat
     7741 gtcaaagcaa actactattt ctcaatcttg gctctagact caaaaggtca ggtcttgaag
     7801 ttggctgaaa tcttcaatgc tcaagatatt tcctttaagc aaatccttca agatggcaaa
     7861 gagggtgaca aggcgcgtgt cgttatcatc acacacaaga ttaataaagc ccagcttgaa
     7921 aatgtctcag ctgaattgaa gaaggtttca gaattcgacc tcttgaatac cttcaaggtg
     7981 ctaggagaat aagatgaaga ttattgtacc tgcaaccagt gccaatatcg ggccaggttt
     8041 tgactcggtc ggtgtagctg taaccaagta tcttcaaatt gaggtctgcg aagaacgaga
     8101 tgagtggctg attgaacacc agattggcaa atggattcca catgacgagc gtaatctctt
     8161 gcttaaaatc gctttgcaaa ttgtaccaga cttgcaacca agacgcttga aaatgaccag
     8221 tgatgtccct ttggcgcgcg gtttgggttc ttccagctcg gttatcgttg ctgggattga
     8281 actagccaac caactgggtc aactcaactt atcagaccat gaaaaattgc agttagcgac
     8341 caagattgaa gggcatcctg acaatgtggc tccagccatt tatggtaatc tcgttattgc
     8401 aagttctgtt gaagggcaag tctctgctat cgtagcagac tttccagagt gtgattttct
     8461 agcttacatt ccaaactatg aattacgtac tcgcgacagc cgtagtgtct tgcctaaaaa
     8521 attgtcttat aaggaagctg ttgctgcaag ttctatcgcc aatgtagcgg ttgctgcctt
     8581 gttggcagga gacatggtga ccgctgggca agcaatcgag ggagacctct tccatgagcg
     8641 ctatcgtcag gacttggtaa gagaatttgc gatgattaag caagtgacca aagaaaatgg
     8701 ggcctatgca acctaccttt ctggtgctgg gccgacagtt atggttctgg cttctcatga
     8761 caagatgcca acaattaagg cagaattgga aaagcaacct ttcaaaggaa aactgcatga
     8821 cttgagagtt gataccaaag gtgtccgtgt agaagcaaaa taaagaatag aagataggat
     8881 ggggaaactc ttgaccagag ggtttcatat cctttttgtg aaaagaagtt tatactcaat
     8941 gaaaatcaaa gagcaaac
//
LOCUS       DAVBCG010000080         8930 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBCG010000080 DAVBCG010000000
VERSION     DAVBCG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.080"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(147..950)
                     /locus_tag="VQ495_001481"
     CDS             complement(147..950)
                     /locus_tag="VQ495_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2531423.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1229..1984)
                     /locus_tag="VQ495_001482"
     CDS             complement(1229..1984)
                     /locus_tag="VQ495_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2531424.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2054..4705)
                     /locus_tag="VQ495_001483"
     CDS             complement(2054..4705)
                     /locus_tag="VQ495_001483"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2531425.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4698..5276)
                     /locus_tag="VQ495_001484"
     CDS             complement(4698..5276)
                     /locus_tag="VQ495_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2531426.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5276..5746)
                     /locus_tag="VQ495_001485"
     CDS             complement(5276..5746)
                     /locus_tag="VQ495_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531427.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(5733..6311)
                     /locus_tag="VQ495_001486"
     CDS             complement(5733..6311)
                     /locus_tag="VQ495_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2531428.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6594..6989)
                     /locus_tag="VQ495_001487"
     CDS             complement(6594..6989)
                     /locus_tag="VQ495_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2531429.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7085..7339)
                     /locus_tag="VQ495_001488"
     CDS             complement(7085..7339)
                     /locus_tag="VQ495_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2531430.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7348..8682)
                     /locus_tag="VQ495_001489"
     CDS             complement(7348..8682)
                     /locus_tag="VQ495_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2531431.1"
                     /translation="MTDEWIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(8682..8915)
                     /locus_tag="VQ495_001490"
     CDS             complement(8682..8915)
                     /locus_tag="VQ495_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2531432.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLTGTPMDKIVRTLEANGYEVIGLD"
BASE COUNT         2516 a         2007 c         1695 g         2712 t
ORIGIN      
        1 tgtgcaatac agaactaaat ccttcggata aattatttgt ctaacttttt ggggtcagta
       61 cacctaaaac tttgatgata tacgttttct tgtgagaata tttacttcat ttttgcctaa
      121 aattcaatgt ttactcagta tttggattat gaaaaatcga ggtctaaatc tagatacatt
      181 ttttctgaag acaaatcatt ttgaccaccg agcaagagat tttcaaaaaa agctgttaaa
      241 aactcagaac gtcgctgtaa aatctttgca ttatctaata ccaaggcatc acgaaaatat
      301 ttggaatgtt gctgaaatgg tgtattatca atatcaaaac caaactcacg aagatactga
      361 atcaaaaaga ccgttactgt ccgagtgttt ccttcgcgaa atggatgaat ctgccagatt
      421 cctgaaataa aatgctggat ttgtttaacc acatccgcct gagttagtgt cgcatatgca
      481 acttgttttt cctgattaaa atcataatct aaggtcattt gaatcatgga gtaatcagag
      541 tacacaacac tttcaccatt caaaacaggt tcattctttg tgatattggt ctgacgaaat
      601 tgccccaccg gaatagaggg ttcaaatata tcttgaaaca actccttatg aatagcaagt
      661 aaggtcgcag gactaaagct aaagcctctt cgagacaata gttctacaat acgtagagaa
      721 accaagtctg cctcttccgt acttgcatca atagtatggt gataagccgt tgcatcctca
      781 taaacctgct cataagtcag ttctccccgg gactgtttct cagccaaaga ttccatatac
      841 gctgatggca ctagattgtc aactttctgc agaccaaaac ctatccgcca taaatcacgc
      901 ttcgcttcat aagacaagtt tggattgtca atgttgtaag ttgattgcat agaaatatcc
      961 tttcaattgt ttttcttcat taccagtaaa tcacgaaatt ggttggaaac tggcttaaac
     1021 tataaaaagt cctcacaata ggttctatga tatatatata tatacttcat gtaaataata
     1081 aaagagttat aatcagcact actgataaat tcaataccga tttcgccatc ttaaaactta
     1141 gtcaaacgaa taccgttgat ttccagttca ttaagtcgtt gacctgtcaa gacttctcgt
     1201 tccttcaact ggaaaagtta agggggaact aatacaaatc ctctacgctt ctatattcaa
     1261 tgatttcatt tttgataata ttctttttaa actcaaaatg ttttaaagga ttatcgatta
     1321 acactaactt tggaatatca tctagatttg tgaccaagga taaaataaaa tccgattgaa
     1381 actccttgac tttttcaaat tcttttgcag ttaaacgcac acgcccctta gattttcgga
     1441 taccttttac ttcggcaagg taagaatact cttggacatc tacctgaaag tcatatccat
     1501 caccataaag acgggcatcg gttaactgtc caccttggaa tttttcttct ttatcaaaat
     1561 gaaggataaa ataattctca gcctccattc ccgtttcctg tagacgtttg aatttagttc
     1621 gaactattgg tttttcaacg atggttatgc ctgttttctt cccttcactg gcaagtaaca
     1681 ttttaactat ttcagcataa ctatgaacat cttcatttcc aaacatcata tcaattaaat
     1741 ctttacgaaa acgataaact tcagctttct gccaccaacc ttttcgatta ttgtcaaaat
     1801 aaggatcaaa taaatccatt ctatttttta caactccagt tgtttcagca attcccagag
     1861 aaaccacata gcgataaaac tccgacttgc tagagcattg aaattctttt ataaaaagct
     1921 tgtcaaactt ggcaagaccg tagccaatta aatttaacaa ttcataatga gggtgtttgg
     1981 acatcacttt ctccatcaat agatattgat ttcattatac caaaaaacac ggctcatcac
     2041 cgtgtttctg tgtttatttc accaacttct tcatctcatc aatacgtgct acggtcgcat
     2101 catacttagc ttggtaatct gcttgcttgt cgcgttcttt ttggacaact tctggtttag
     2161 cgttggcgac gaagcgttcg ttgctgagtt ttttgccaac catgtcgagt tctttctgcc
     2221 atttggcaag ttctttttcc agacgtgcca attcttcttc gacattcaag aggtctacca
     2281 gtggcaagaa gatttcggct cctgtgatga cgcttgacat agccagttca ggtgcaggga
     2341 tggttgatgc gatttccaag tgttcaggat ttgtgaaacg tttgatgtag ttgacattgc
     2401 tgttaaagaa ggcttccaag tcgctatcgc ttgtcttaac aaggatagtg ataggcttgc
     2461 ttggagcaac gtttacttcc gcacgcgcat tacgaacggc acggatcaag tctttaaggc
     2521 tttccacacc agtgtgagca gcaaggtctt caaaggctgg gttgacagtt gggtattctg
     2581 ctgtaacgat agatccttct gagatttgtc caaagatttc ctctgtcaca aatggcatga
     2641 ttgggtgaag gagacgaagg atcttgtcca aagtgtatag tagaacagaa cgtgtgatga
     2701 ctttctcttc ttcgttatcg ctataaagga cttccttggt caactcaaca taccagttgg
     2761 caaattcttc ccagatgaag ttgtaaagga tatgaccagc cacaccaaac tcgaacttat
     2821 caaagttttc agtaactttt gcaatggttt cgttgagatt gtggagaatc cagcggtccg
     2881 tcacattacc agcctcacct gttgcaactt ttgtgacatt gtcatgcgcc acatccagcg
     2941 tcaaaccttc attgttcatg aggatatagc gagaaatgtt ccaaattttg ttaataaagt
     3001 tccatgaagc atccattttc tcgtaagaga aacgaacgtc ttgaccagga gctgagccgt
     3061 ttgacaagaa ccaacgaaga ctatctgtac catatttatc aataacatcc attgggtcaa
     3121 tgccatttcc aagagattta gacatcttac gtccttcttc atcacgaatt agaccatgga
     3181 taagagcatt tttaaatggg ctctttcctg taaattcaat tccttggaaa atcatgcgtg
     3241 atacccagaa aggaataata tcatagcctg ttacgagtgt tgaatttgga tagtagcgtt
     3301 taaagtcagt agtttcggta ttaggccagc ccatggttga gaagggccag agggcagagc
     3361 tgaaccaagt gtcaaggaca tcttcgtcct gtttccagcc atcgcccgct ggggcttcct
     3421 cgccgacata gatttcgcct gcttcattat accaagcagg gatttggtga ccccaccaga
     3481 gctgacggga aatcacccag tcgtggacat tttccatcca ttgcaggaag gtgtcgttga
     3541 agcgcggtgg gtaaaattcc accttgtcat ctgtgtcttg gttggcaatg gcgttcttgg
     3601 ccaattggtc catcttgacg aaccattgag tagacaagcg tggctcaact acgacacctg
     3661 tccgttctga gtgaccgact gaatggacac gtttttcgat tttgacaagg gcaccgattt
     3721 cttccaactt agcaacgact gccttacgag cttcaaaacg atccatgcct gaaaattcaa
     3781 aggcaagctc gttcatagtt ccgtcgtcgt tcatgacgtt gacttgtggc aagttatgac
     3841 gttggccaac caagaagtca tttggatcgt gggcaggtgt gattttcacg acaccagtac
     3901 caaacttagg atctgcgtgc tcatcaccaa cgattgggat taatttatta gcaattggaa
     3961 ggatgacatt tttaccaatc aagtccttgt agcgcgggtc ttctgggttg accgcaaccg
     4021 caacgtcccc aaacatggtc tcaggacgag ttgtagcaac ttcaagggcg cgtgaaccat
     4081 cttccagcat gtaattcatg tgatagaagg caccttcgac gtccttgtga ataacctcga
     4141 tatcagaaag ggctgtgcga gctgctgggt cccagttgat gataaactca ccacggtaga
     4201 tccagccttt cttgtaaagg tccacaaaga ccttacgaac agcttttgac aaaccttcgt
     4261 caagagtgaa acgctcacga gagtagtcta cagagagccc catcttgccc cattgttcct
     4321 tgatagtagt ggaatattcg tctttccatt cccagacttt cgtcaagaaa gactcacgac
     4381 caaggtcata gcgggaaatg ccctcaccac gcaagcgctc ctctacctta gcctgagtgg
     4441 caatccctgc gtggtccatc ccaggaagcc aaagggtatc aaaaccttgc atgcgttttt
     4501 gacggatgat aatatcttgc aaagttgtat cccaagcgtg accaaggtga agtttacctg
     4561 taacgtttgg tggtggaata acgattgaat aaggcttagc cttttgatcg cctgaaggct
     4621 tgaaaacatc agcatcaagc catttttggt aacgaccagc ctcaacctcg gctggattgt
     4681 atttagatga aagttcttta gacatgtgtg tgtcctttct ctattttgtt tattttattt
     4741 tgaatttgct tagcagcttc ttctgcagac aaattcgtat tatttatttt aaagtagtgg
     4801 tgcaactcat tcggttgata ttgggaattt aattgaagtg tttcagcggt ctctaaaatt
     4861 tctctttcag atacctcaat atgtcgtttt aagggtttgt gctttaatcg attctccgtt
     4921 cgatttcgac gtatgcactc ttcaagactt gtttccaact caacaaaaag aatctcttga
     4981 tgataatcat ccaacaaatt ctgaatttgc tttaaataca tcagttgata ctgatttgaa
     5041 aaatcaatta catctgttaa aattactgat cgctgatttc ttgcacttgc tccaaggaat
     5101 gaaaaagtga ttccacgaac aaattcccac atttcctctg tatagtcctg atagatttct
     5161 agtgcaaaat caatggcttg atggttataa aatagggtag catccgtcag tcgagataat
     5221 tcttgaccaa tggtcatttt tcctgatgct ggagcaccaa tgatgaaaag atgcatcaaa
     5281 tcacctccca ctcactcttc agcaaggcat atctcaaatc atcacagcag ttgccttgag
     5341 catctttacg gtctcttatg cgagcttcga gggtaaagcc aagcttttcc gcgactcgtt
     5401 gactttgaag gttatatcca aagcaagtta gttcaatctt gtgaagaccc aaatctttaa
     5461 aggctaagtc aatcaaggca cgcgcagctt ctggcacata acctcgcccc caatagtctg
     5521 ggtgtaaggt atagccgatt tccagcacat catcttcatg gcgatggttg aaatcgacag
     5581 agccaacgat cttatccgtt cctttgacga caatcccgta gcctgctgga taggcgacct
     5641 ctggcaaact agcatagtca aagatatcct cagcatccgc cacagtacgg actcgtaaaa
     5701 cgagacgttc tgtttcgatt ttatctggca gctcagttct tgccatcctt cttcctcgct
     5761 tttttgatga aactgccctt catatctaca cgcttatcca gatagcgata aacgcgctga
     5821 tatccatctc ccatgaaata ggttggggca aacagttgat ttttaaaatg tcccttttca
     5881 tccaggagtt ctggggcaac aagtcgctca agaatcttgg caaagatgtg gcaaataccg
     5941 tcttcctcaa caatcctatc tacccgacaa tctaaaacaa gtggacaggc gtctaaaata
     6001 ggagtctgag ttcgttcaga aatttcataa tgcactccca aacgttccaa tttctcctga
     6061 tgactgataa aaccagcctg ctccatcgca agcatagaag tttcatcaga aatattcaca
     6121 gtaaattttt gatactgttt gatctgctct gcggcattct ctctcgcaac gactccaatc
     6181 acaacccaat ctcctagact atacgaggaa ctacaggtcg tgatgttata gccaaaattc
     6241 tgatcttgat atcctaaaat aaaaacagga aaaccataat atagtttatt agttttaaat
     6301 gattgtttta taacaacctc ctttgactaa gacacaaaag aaaagccctg ccatctacat
     6361 gacagggacg aatgtattta tccgcggtac cacccaattt cgggccaagc ccgcatcttt
     6421 agtctttaaa ataaaaagac agaaatattt cattttttca gtcattagca accagttttg
     6481 acacatttgt ggacttctca gcgccgccac tttctgtaaa atgtgggatt caaaacacct
     6541 ctaatagctc tattgtagca agtttttctc ggaaatgcaa ggctcaagaa aaattacttg
     6601 aacttgatgc cattttcttt caatttcttg atggtagctg ggccgattcc tttcaaggca
     6661 aggagttctt tttcagtcca gtttttgaaa tctgaagcag acttgattcc ttcatcatag
     6721 aaagtcttgg cacgatcaag tggaagtcca cccaagtttg ctgcaaaatc ttctacagaa
     6781 ttagctactt tttgagcttg ctctttggta gcttctactg cttgttcaac ttttttagaa
     6841 acagccttac cagctgcgct tgctgactgt tctgcacgct tcacgacttt ttttgtctca
     6901 tctacaacct tagtcacagt acttgaaaag gcacctgcgc gacggaggcc atttcgtaat
     6961 tgttttttac gattgagttt ctttgacatg ataaaatcct ttcaataaaa aatccctgct
     7021 ctgacaagga tttaacataa acattctatc aagattttag tgaaaaatca agaatctttc
     7081 tcgtttaata atttcgctca ccaaacaagc cttctggcaa atcatggaat cccttgccta
     7141 ctttgaagtt ttcaaactta ccaagcaaga actgataagc atccaagcga ctctcgtagc
     7201 gaatcatggc atctttggca cgctcgtcct ggtcttcttc gtaaactttt tgactttcct
     7261 tgtgcgccat gtcgttaatc atgatttggg tattgaccca agcttcaaat tccttcataa
     7321 attcttgttc gtaactcatt tttctcctta ttctaatcca cgaaaatagt ccgtatcaat
     7381 ctcacggtag ggctggatat cctgaacata ctccaacact ccttggaatt ctccgtcttc
     7441 atcgtgtact gcagcatagg tgatgtggac aaacttacct cgcgactcag acttgaacca
     7501 catttcatac ttgtctttgc ttccctcacg aagcctcttc atgatagttt tgaccttgtc
     7561 caagtactta ggcggatggc agagttcgac attgcgcccg acttgagacg gcgtccgttt
     7621 gaaaatcatc tcatcagctg gcgtattgtc attgtaatac tggaaaatat cttctttatt
     7681 gacaaaggta atctccatag ggagatgatt gaggatgaga ttggcctgct cgactgaaag
     7741 atagccatta ccaaaagcct gttgactatg gcggtccagc acagcttcct tttccttagg
     7801 ggtaaaggta atggtaaaat ggccttctgg cgtatctatg acttgttgaa cttgaccttc
     7861 tgccgtatct agctgtacag gctcctctgc aatcttttcc tcaataaagc tctgtcgttc
     7921 tggcacccat ttctctgacg gacggatgat ggcatagcca taggcatcgc tctcctccgc
     7981 aatctgaagc cagtcatcct gagtaaaaga ctcaaggaga atcatgagga ggatggactc
     8041 ttccttgaaa atcatacttt caaactctgt cgcaaaagct tcaaaagctt cctttacact
     8101 gctaattgac acttctggta gtgacttgac tgtcgttaga gctgtttgaa agagttccct
     8161 aatctgatca tccactcccc acataacttt gggaggtgaa tcgtgtccat agcgctccat
     8221 gataggaaag aagagttctt ccttacgttg gtaatggatg tcaaattgcc ccacaagccc
     8281 catctgacgc accaagccct tgcgcatctc cgcaagcatt tcctcgtctt ccatcaactc
     8341 ataggtatct aacaatctac gaatgcgaat caaagctgca cggagggcca aattttcttc
     8401 cttaaagaca cgaactgggt gcccagggtg ttcggtatct aaaacttcga cacctttgat
     8461 agcattttta aaaagattgg catggacatc acagagttcc ataacatctt caaaagtgac
     8521 gcccgaatcc gagttcatca gctcgtgctc cataagggaa atctcgatgg ctgacacgcc
     8581 cgtaaaggtc gcatcaaagc gatcttgaac cgactcagga gaggcgccat tgtgcaattc
     8641 taacaaaata tcccgtagga tatgaatcca ttcatctgtc attagtctaa tccaatcact
     8701 tcgtagccat tcgcttccag tgtgcgtaca atcttgtcca taggagttcc tgttagctta
     8761 gaaccctgtt taagtgatac tttacgacca actgtattgc gcattaaggg attggcaagg
     8821 ggtttaaaac ccaactccac tagaatttcc aagacttctg gatgcttgtc caccacttct
     8881 gcaacaggaa ttgacacatc gatgatattg tccatgacga cctccattgt
//
LOCUS       DAVBCG010000081         8885 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBCG010000081 DAVBCG010000000
VERSION     DAVBCG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.081"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            168..854
                     /locus_tag="VQ495_001491"
     CDS             168..854
                     /locus_tag="VQ495_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2531433.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKTAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMAAISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            855..1787
                     /locus_tag="VQ495_001492"
     CDS             855..1787
                     /locus_tag="VQ495_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2531434.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1798..2727
                     /locus_tag="VQ495_001493"
     CDS             1798..2727
                     /locus_tag="VQ495_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2531435.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2741..4180
                     /locus_tag="VQ495_001494"
     CDS             2741..4180
                     /locus_tag="VQ495_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531436.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4249..5826
                     /locus_tag="VQ495_001495"
     CDS             4249..5826
                     /locus_tag="VQ495_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2531437.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5886..7346
                     /locus_tag="VQ495_001496"
     CDS             5886..7346
                     /locus_tag="VQ495_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2531438.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            7368..7823
                     /locus_tag="VQ495_001497"
     CDS             7368..7823
                     /locus_tag="VQ495_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2531439.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            7910..8315
                     /gene="cdd"
                     /locus_tag="VQ495_001498"
                     /pseudo
     CDS             7910..8315
                     /gene="cdd"
                     /locus_tag="VQ495_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            8328..8777
                     /locus_tag="VQ495_001499"
     CDS             8328..8777
                     /locus_tag="VQ495_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2531440.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
BASE COUNT         3093 a         1153 c         1665 g         2974 t
ORIGIN      
        1 gtgtattgaa tctataacag tacgccttaa atgctaaaat atttctataa attaatttaa
       61 ctttcctgat agggctgttc atatcttatt tcaattcact atataaataa aattgtagta
      121 gatgttattg aattagtaaa aaaaataaaa aaacggaggt tttctgaatg gaaactaaaa
      181 aaattaaaaa tttaaaaggt caaataattg tttcctgcca agcgttagaa ggagagccac
      241 tttatactcc aaatggagga gtgatgccgt tattagcaaa aaccgccttt caagcaggcg
      301 ctaaaggtat tcgtgctaat tctgtccgcg atatttcaga aataaaagaa gaagttgact
      361 taccaattat aggtatcata aaaagagatt acgatggctt tgaacctttt atttcagcaa
      421 ctatgaaaga gattgatgaa ttagttagtg aaggagtaga cattttagct ttggattgta
      481 ctaatcgttc aagaccaggt tatgataata ttactgactt tattcatgat attaaagtca
      541 agtatccaaa ccaattatta atggctgcta tttctacttt tgaagaaggg aaagtagctg
      601 ctgaatcggg agtagatttt gtgggtacga cattatctgg ttatacacca tatagcccta
      661 aaaaagataa tcctgatttt gagttagttg aaagattggt aaaagagctt gacgtaccag
      721 ttattgctga aggaagaata agtactccgg aacaagctag aaaaatgtta gatttaggtg
      781 cttacgcagt ggttgttgga ggagctatca ctcgtccttt ggaaatagcg aagaagttta
      841 ttgaggtagt ttagatgaat caagaaatat taaaaaagag taagattcaa ttgacagttg
      901 aacatattca aaaatattgg attttatatt taatgatgat tccagcaact gttctactaa
      961 ttttatttac gtatggacca atgtacggca ttattatggc ctttcaagac tttactgtct
     1021 tcaagggata tactggaagt ccatttgtcg gtttaaaaca ttttcaaaga ttgttctcag
     1081 atcctttgtt ttatagattg ttcaagaata cgttcatggt aggggtttta gattttttat
     1141 tctttttccc agctccgctt atttttgcac tcatattaaa cgaagtcaga aaagtaagat
     1201 ttaagagtgt agttcaatct attagttatc ttcctcattt tattccccta gtggttatgg
     1261 ttggtattat ttttgaacta tttggttcat atggtattat taacagttta ctttcatcac
     1321 ttggaatgga gccaataagt ttctttacaa aatctgaatg gttcttgcct ctctacattg
     1381 gttcaggagt atggaaaaca ataggttggg gttctatcat ttatatggga gcattaacca
     1441 atatagattc aactttatat gaagcagcag atatggacgg ggctaataga tggcacaaaa
     1501 tgtggcatgt aactctacca agtttaagac ctacagtagt tactttgttt atactaaatg
     1561 ctggtggtat catgcaagta ggatttgaaa aggtattctt gatgtctagt cctgcaacat
     1621 atgaagtatc tgatgtatta tctacttatg tttatagaca aggtatttta aattctgatt
     1681 tcagttactc agctgcagta ggattattta ataacatagt agctttgtta tttgttttat
     1741 tagctaataa gattgctaaa aaacttggcg aagaaggaat tgtataaggg gggtaaaatg
     1801 attttttcaa aaaatacaaa aaagagtaat agagacaaag aaaccttcgt tgattatctc
     1861 tttatattca ttttaataat aataacctta cttatgttgt atccattatg gtgggtagtg
     1921 atgacgtcac tttcagaacc agtttggcta tctaaaaacg ttgttaaatt atttccctta
     1981 aaaatagata ttactgcata taagtttatt tttggggatt ctaaactatg gcaagcatat
     2041 ggtaatagtg tattgtatgc tgcactatct acaattgtta gtgttgcggt atgtagtatg
     2101 tatgcctatc cacttacaat tcctgaaatt aggggtaaaa aattctggaa tactcttatt
     2161 ttgattccaa tgttctttag tggaggaatg atcccgcttt atatacaagt tgcaaacctc
     2221 ggtttattga atactgcttg ggctcttgtt ttacctggtg ctgtttctac atattatata
     2281 attttgttta gaacacagtt taaatcaatc ccaatggagc ttcgtgagtc tgctagaatt
     2341 gatggggcaa gtgaatttag aattttttgg aatttagtat taccattatc aaaagttatt
     2401 ttcatgatta tagcacttta ttgcatagtt ggttcatgga atagttttac tcctccatta
     2461 ctttatttga atgacgacac taagatgcct ttatctattt atttgagaaa tttagtcgtt
     2521 acaaatacaa ctgatactgc tgaattatca ctttctgcag gtaatacata ttaccaacaa
     2581 attgctgctc aaggtggagg tattggtatg cgtattgcta ttaagatggg tactattgtt
     2641 gttagtgtta ttccaattat gatgatttat ccatttattc agaaacattt tgttaaggga
     2701 atcctgctag gttctgtgaa aggttaataa ggtaattatt ttgcaaaatt taaaaaaata
     2761 tcttttatta gatagcggtt tagtagttgg gtatacatta gaaaatgctc aattaaaatt
     2821 aaataagcta aaaaaagata ctgaaaacaa tccactattt ggtgaagagt attttgccga
     2881 aaatcctaaa ttatgggaag ttcgttttga taattcatat ccaaacgtta tctatgataa
     2941 agaattgaaa ttatatagat gttggtatca aacatttgtt agtgatgaag caagcgaaga
     3001 gactccttta gcagaacgtg gagagaagga atacattgtc aagtcatcta gaatgacagc
     3061 actttgctat gctgaaagta aagatggagt aaaatgggaa aaaccaaatc tcaatctagt
     3121 aaaatttaaa ggttcaaaag ataataatat tgttctgatg catgcacacg gtgcaggtat
     3181 catgctagat gaacaggagt tggatcctaa gaaacgatat aaattagttt caaaattaga
     3241 attttctccg aatcttcatt acatggttgt tgttttttca gaagacggta ttcattttga
     3301 cgaacctatt ccatggaaaa aatggaatcc tgcagcagat agtcataatt ttccatttag
     3361 agatccaaaa actggtaaat acgctgttat tacaagaacc tgggcaaata acgtacgtct
     3421 aagtgcaatt actttcagtg atgattttat taattggtca gaacctaaag aaatccttag
     3481 gggagatgga tttgaagatc aaatttattc tatgccagta atttcatacg cggatatcta
     3541 tttaggtttt gcttcaatgt accatgaagg cgacacttta aatgaaaact atgataaagt
     3601 agacttagaa ttgaaattct ctgctaattt ggaacaatgg gatagtgtat gtaagggcga
     3661 ctatctcatt gaaaggggag gtggagtata cccgcagaat gctgattttg atgctagttg
     3721 tattttcacg gctgctccaa taattgaaga tgataagatt tggttctact atatgggtgg
     3781 aaatggtaat cacactggct tccgagaaag ttcattagct agaggatata ttaagaaaga
     3841 ccaatttgct tattatacta ataagagaga aggaattcca gctaaaatat ctacagttag
     3901 cttccaaact tacgatgatg aaatttatat cttagctgat attcctgaaa atggttatat
     3961 taaagttgcg gtaggtacta aatacggtaa tgcttattca gatttcggtc aggaagatat
     4021 ctctcttgtt caagtttccg atggtaagta taagttggaa tttaataaaa aatctatctt
     4081 agatttaggt aattctccag ttgcgttcaa atttgaaatc gagaatgcaa aattgtatgg
     4141 tattgaaggt agtattcagc aacactatct caaatattaa ccagaataat tcgttaacaa
     4201 ataatctcta tattagtgga gatagatata taaaatggag gtcttattat ggtaaagctt
     4261 aataaggtat taaaagggag tgttgcattc ctgagtgcaa gtttattgct agcagcttgt
     4321 ggcaataacg ctaattcaaa gcccgctgaa aaaacagctg atggtaaaac gaaaatttcg
     4381 tttctatcta catggcaaat taatgatgtt cgtccgccta aagatgaaac acttgttggt
     4441 caatggttag aagaacagct aggtattgat attgatcttg taactattcc tggcgaggga
     4501 gtaagttcta aagtaaattc tcttattact agtaaccagt taccgactgt tttgttgaca
     4561 acaggagata agagtgatat cgctggtatc aacaaacttg gtaaacaggg tgcattctta
     4621 gatttgtcac aacacatgga caaactacca aactataaga agtaccttga aaaaaataat
     4681 gcattagctc aaatagaaga tgatgaaaaa catatttatg ctttcacaaa attctttact
     4741 gatgagaata tcatgtatac tacaccaatt ctgagaaaag acttacttgt tggttcagaa
     4801 tttgaagatc tttcaaaaat taaaactgtt gatgactata caaaagtttt gaaatatttg
     4861 actgaaaaac aaggttctcc agcatttatt caacgtaatg gctatgaagg cttcatgaag
     4921 cgagttacac cattgtggaa tttaagccat cgtacatact atgactatga atcagattca
     4981 tataaacatc cagttgaaca acctcaattg aaacaattcg ttgaatggtt gaaagaatta
     5041 cgtaaagata acgtacttca ccctgactgg gcagttatga aagatgaaac ttgggaaggt
     5101 ctattggctt ctaacaaggg ttcattcact gttgaccgta tgaatatcat tggagataat
     5161 aactttgata aatcatttga ttggcagcca cttgtttatc cagaaattaa tggaaaacgt
     5221 tatttgcaac cgaaaacacc gtacatttct gattcaggtt gggtaatcaa tgccaatgca
     5281 ccaaaagaag aaattgataa agcgttagaa ttctttaatt tcttatacgc tgatgaaaat
     5341 caagaaaaac ttgctattgg atttgaaggt aaaacattta cacgtgaaaa ccctaatact
     5401 caagcaggta ttaaatggtt gattcaaatt tacggtgaaa atgctggaaa tgataaagca
     5461 gaactgctgt ttaaacatgg taaccaagca tttactcgcg ttcaaactaa attagatatt
     5521 gaggcacagc caggaacatt ccctaaagtt atgtatgatc aagtagataa aattaagaaa
     5581 gatttaggtg gttttagacc agatactccg gttctttcct ttacaactga agaattagat
     5641 gttttaacta aatctgaagc aggattaaat actttctttg atgaaaatat tattaaattc
     5701 attgaaggac aaagagatat gagtgagtgg gattcattta tcaatgaagc taaatctaaa
     5761 gacttggata aagttacaaa agtatataat gatgcctaca aacgtgtcaa agatagaatg
     5821 aaatagacta aatggtagct ctctggtttt tcagagagct actactgcct gagggggagt
     5881 aagctttgta tgatgtagta attataggtg caggaagttc gggcgcattg gctgcaattg
     5941 cttcggggcg tctagggtta aaaacttgtc taatagagaa gggaagtaga attggtggag
     6001 ttcctattaa tacactaatg gggtcatttg ctaatctata ttttgatgat tctggtagag
     6061 taaatgcaag taaaattgtt ggggaattaa tagaacgtat tatcaaaaaa ggtggtactg
     6121 tctttaactc tatggaagaa ttgatgaatg taaatgattt ttatcagatt acaattccct
     6181 atcaaccaga agtttatgaa tcggttttaa ctgatatgtt gattgagtct aaagttgata
     6241 ttttactaaa tgttgaagtt aataatatta tttatgatag taaaaatatt aaaagtcttg
     6301 aaattttaag taatcaggaa gttaaattga ttgattctaa agtttttatt gatgctacag
     6361 ggaatgcaac aattgcttct gctagtggag ctgatgttta tgaaaaaaat tcttcttacg
     6421 gttgtttaat gagagttgga ggagttgata tctcaaagac tatagaacat attgaaaaaa
     6481 caaagcagtg gaactatata gaaggatata atcagtggtt agaagaagta ttagcaaatc
     6541 gaaagtacag agccactgga gcagttttaa aagatccagt ttcttatgat catgccccta
     6601 tgctttcaaa agacgaccat tttatgaatg atgaaaaatg gaattacatt aaagaaagat
     6661 gggaaaaatt tagatttatt tacactttag aagtatcact atttaggaat gaattaaaaa
     6721 aagctgtaga atctggggat tttaattttg aaatttatca tgatgataaa tctggagtca
     6781 ctatgaatgg cgacggtata agctatggtt catggggtca aaatgtagca ttaattaatg
     6841 ttgcaaaagc atttggattc aatcccaaat atatggaaga tgaatcacat gctaatattc
     6901 tttctagaaa atataattta acatttttat cttttcttaa aaaatatgta ccaggttttg
     6961 aaaattcttt tatccttgat cagggaacta gagctactaa cagatcatgt aggcacattg
     7021 ataattcatc tatgatggac gaaattcttg attttccaat gtatacattt aagactatgt
     7081 atagctatca aactcctaaa gaaataacgt acaaatcaat tgtaggtgga aaattgaata
     7141 atttgtttgt aataggtaaa ggtgcttacc aatccgaaag ttttagatca caaattagtt
     7201 gtatgttaat ggggatatca tcagcagcag cggcaaaaac tattattgaa gataaaagca
     7261 atactctaaa cattaataga gatttgttta aatcgaactt agatatatta tttacatggg
     7321 agttttctaa ttggattact gattaacaaa taataaggga atattaaatg aatgatacta
     7381 ataaaaatca atactttgtt tatgaatttg atactagctt tgtagaaatg tcttattctc
     7441 agttttttga aggatattgt attctttttt caaaagaacc ctatccttct ttatctgata
     7501 tgccaatgga atttagaaaa gaatttttaa tggaaatgag tatattaggt gatgcgatgc
     7561 ttgaagtgtt aaatgcctat agaattaatt atgatatctt aggaaattca acacctgtac
     7621 ttcatgctca tttatttccg agatacatgt gggaagagga agagagaaga aaaactgtag
     7681 ttcatagata tgattcttca gtttttagca atcctgagtt tgaatttact atagaaaagc
     7741 atggaccaat tattaaaaaa ctgaggtctg ctattgatag acattataga gagtatcaac
     7801 aattattaga tgattcaaac tgaactccac attttcgatt agatttaata ccttgtatat
     7861 tacaactagg ttgtggatga tataattttt tgaaaaatat attttaggaa tgacttataa
     7921 agcaattata tcagaggcta ttaaatcaat ggccaaagct tatgctcctt attctgattt
     7981 taaggttgga gctgcgttag taactgaaga taaaaaattt tttctggatg taatatagaa
     8041 aatgctgctt tttctcctac ttgctgtgca gaacgagtat ctttttataa agctgtattt
     8101 aaaggagaac gttcgttttc aagaacatgt attgtaggtg gaaaaaatgg tatcttatca
     8161 tcttttatag caccttgcgg agtgtgtcgc caggtaatga tagaatttgt aatcctgatt
     8221 catttgaagt tatattggct agtgatgttg atacttataa agtatataaa cttaaagaac
     8281 tactaccagt tagttttaat caagaaaatt tataacagga gggaactatg gaattagttg
     8341 aatttaaaaa tggacatgtt aatacggttc atgagcatat cgcaataacg aaagtaaaag
     8401 agtttctaaa taatcatgat atattaaaag ataaaggtgg catacataaa atttctactg
     8461 atttctttta caatataatt gatatggaaa ctacaactga agagaataga ccgtgggaaa
     8521 gtcataagga atattatgat gtccattatc tgttaaatgg agaagaaaca attttatata
     8581 acttcttgtc tcaaatggaa ttgtctgaat ataaagtaga tgatgattgg caacaaatga
     8641 atgggacagc tttatttagt attaaattaa agaaagatat gttacttttg ttggaaccaa
     8701 acgatgcaca tagaacaggc ctattggttg aagaaccact taatattaag aaagttgttt
     8761 ttaaggtaaa aatttaactg aaagtacttt cggggattaa gaattttaaa atatttaaga
     8821 aattgtattc taaagttcta aaattataga tatttaattt aaagcttgtt aaaatagaat
     8881 agtaa
//
LOCUS       DAVBCG010000082         8781 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBCG010000082 DAVBCG010000000
VERSION     DAVBCG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.082"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQ495_001500"
     CDS             complement(135..1103)
                     /locus_tag="VQ495_001500"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2531441.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQ495_001501"
     CDS             complement(1248..2063)
                     /locus_tag="VQ495_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2531442.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQ495_001502"
     CDS             complement(2088..2585)
                     /locus_tag="VQ495_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2531443.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ495_001503"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQ495_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2531444.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQ495_001504"
     CDS             complement(4033..4548)
                     /locus_tag="VQ495_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2531445.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQ495_001505"
     CDS             complement(4550..4993)
                     /locus_tag="VQ495_001505"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2531446.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQ495_001506"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ495_001506"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQ495_001507"
     CDS             complement(5180..5647)
                     /locus_tag="VQ495_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2531447.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQ495_001508"
     CDS             complement(5848..7134)
                     /locus_tag="VQ495_001508"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2531448.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQ495_001509"
     CDS             complement(7365..7601)
                     /locus_tag="VQ495_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2531449.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8664
                     /locus_tag="VQ495_001510"
                     /pseudo
     CDS             7867..8664
                     /locus_tag="VQ495_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2817 a         2097 c         1407 g         2460 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcagta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagacataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacaga ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
     8101 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
     8161 gggagatgga gatgagcttt attgctcagg actttgataa actcaatatc ataactgttc
     8221 ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta tcaagcgcta
     8281 ctggaagctc attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac
     8341 ttttcgtatg cacttgacta ataaagaaat tctagataaa cttttgagct attccgaaga
     8401 cttgaaacac cactataatc tctatcagct cttgcttttc cacctctgtt tcagactttg
     8461 tttaaaacct tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc
     8521 aacgccaaac tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt
     8581 tttaggaact ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg
     8641 ctttcttcaa cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc
     8701 aattgaaaca agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct
     8761 ttttgaggtt ctttttgata t
//
LOCUS       DAVBCG010000083         8656 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBCG010000083 DAVBCG010000000
VERSION     DAVBCG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.083"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            20..199
                     /locus_tag="VQ495_001511"
     CDS             20..199
                     /locus_tag="VQ495_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531450.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            336..515
                     /locus_tag="VQ495_001512"
     CDS             336..515
                     /locus_tag="VQ495_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2531451.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIGKQAGL"
     gene            552..1004
                     /locus_tag="VQ495_001513"
     CDS             552..1004
                     /locus_tag="VQ495_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2531452.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            1295..1765
                     /locus_tag="VQ495_001514"
     CDS             1295..1765
                     /locus_tag="VQ495_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2531453.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            1784..2872
                     /locus_tag="VQ495_001515"
     CDS             1784..2872
                     /locus_tag="VQ495_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2531454.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            2853..3281
                     /locus_tag="VQ495_001516"
     CDS             2853..3281
                     /locus_tag="VQ495_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2531455.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            3419..4369
                     /locus_tag="VQ495_001517"
     CDS             3419..4369
                     /locus_tag="VQ495_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2531456.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDMTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            4371..5114
                     /locus_tag="VQ495_001518"
     CDS             4371..5114
                     /locus_tag="VQ495_001518"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2531457.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            5125..6921
                     /gene="pepF"
                     /locus_tag="VQ495_001519"
     CDS             5125..6921
                     /gene="pepF"
                     /locus_tag="VQ495_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2531458.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            7167..7721
                     /locus_tag="VQ495_001520"
     CDS             7167..7721
                     /locus_tag="VQ495_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2531459.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPGFPKNRVHEVFEILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            7832..8500
                     /locus_tag="VQ495_001521"
     CDS             7832..8500
                     /locus_tag="VQ495_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2531460.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         2482 a         1546 c         2081 g         2547 t
ORIGIN      
        1 aaggcgacgt tgacgtggtt tgaatttgat tttcgaagag tatcagtttg taaaaattgg
       61 agataaaaaa tatagagtac gcagtggtcc tatgattcat tcaactccgc ctcaatctgt
      121 cttagatcga gatactttta caattgacta tacagatgat gaattgctcg ataaagaagc
      181 agtttttact actcattaga aaaataggtc tgaataaatt ggtttccctt agaaccacat
      241 ctaggggctt ttatgtataa tagaataata agtatcactc ctttacaata cgtgtaacac
      301 gtgttataat ggtgttgtca ggaggtaaaa gcgctatgcc tatgacacaa aaagagatgg
      361 ttaaactctt tacggcccat ggttggataa agactagagg cggtaaaggc tcccatatta
      421 aaatcgagaa gcaaggggaa agacctatca caatccttca tggtgagctg aataagtaca
      481 ctgaaagagg gataggaaag caagctgggt tgtaaaattc agtccctgct ttttgatatg
      541 gaggtagtga aatgttagtt acgtatccag ctttatttta ttatgacgac acagacggaa
      601 cagaagcgac ttattttgtc catttcccag attttgaata ctcagctaca caaggagagg
      661 ggatttctga ggctttggct atggggtcgg agtggctagg gataactgtt gcagatttga
      721 tagagagtga tggagagtta cctcagccat cagatatcaa tagcttgtct ttaattgata
      781 atgatccttt taaagatgat gaagatttcg tgtcaaccta tgaccttgat aaatctttta
      841 tttctatggt atctgttgat gtatcagaat acctaggaag tcaggaaccc attaaaaaga
      901 ctttgaccat accaaaatgg gcagataagt tgggacgaga aatgggactt aacttttctc
      961 agactttgac agacgctatt gcagataaga aagttcaagc ctaagattaa gcgtggtatc
     1021 gtggtaatag tcaataaaaa agcacgtcca cttgtgctag ttactcgcct attgatttta
     1081 tagatttatt agccctttcg agggcttttt atattgattt ttatgagaaa tgaagaaatc
     1141 agacttttaa gaaaatcact gataccaagg gtttaaatga ggtaatatgg tataattagg
     1201 acataaaata attttgtggt aagatggtag tatctatttt agcatatttc cgagcaatcg
     1261 gggcgattaa agagtcgcat agaaagagga caaaatggca acatacggat ttttagatgt
     1321 tttagaggaa gagttggaca agaactttcc ctttgacttt gagattagct gggacaaacg
     1381 caaccatgcg gtagaagtga gttttctatt ggaagcgcaa aatgctgcag gtgtggagat
     1441 ggtggatgaa gacggagagg tttcatcaga tgacattctt tttgaggaag cggtcctttt
     1501 ctacaatcct gctaagtcaa cagtcaatga ggaagactat ttgactgtta ttccttacct
     1561 gcctaaaaaa ggattttccc gtgaattttt agcttatttt gtgcttttcc ttaaagatac
     1621 tgctgaggtt gggctagatg tcctcatgga ctttttggaa gacccagaag cagaggaatt
     1681 tgtcatggaa tggaaccaag aagtctttga agaaggaaaa gttggcttgg aagagggaga
     1741 attttatcct tatccaagat actaggagtt ggttggaggt gttatgaaga aaattgggac
     1801 ttatttggtt tatgtgctag ctgttgtctt tattatgcta gcttttgctt gtggaacaat
     1861 cgcatttgca gagttggggt attccgcagt tttagccttt acttttggtt atgccttcgc
     1921 tcttctaagc atgtatttaa tctttattct tcatgagctg ggtcatgctt tttgtggcta
     1981 cttgacaggc tatcggctgg tggcttttgg attaggacat tttattttga ccaaaaagtc
     2041 aggcaggttt catcttagta gaacagccat tctgaaaaat gttggtgctc aatacattgg
     2101 tttaaaagag gatgaaagcg atcaaagaat catcctgatg ctttcaggag gcttgatggt
     2161 tcatctcggc ttgatattat tggcgatagt gtttggattt ttgacaagaa gctggtattt
     2221 tgcagggact tggatttttc ttaatttatc tttcttccta aataacattt tgccagtcga
     2281 catcaccgat ggagcaaaaa tttgggaatt actacaacac cctgaaaata ccaaatacgc
     2341 ctacctggta ttgagacatt ctgctcaaac tttgctggct cctcaagaat atgatttgaa
     2401 agactttgtt caagcagttc ctgaggatgc acgagggagt tttgcggatg gtgtattagg
     2461 tatgcaaggt gaagtttcca ttttggaagg gaaggaagaa gtagctaaac agcagtttca
     2521 agcattactt gagaggacaa ctactccgat gatgcagaca gttgcccaac tatccctgct
     2581 tcatatcgcc ttgttggaag gagattttga gcaagcggag caatatgcta gtattcgaca
     2641 ggtcaaatcg tttctgtctc tcaaactgtc caatcttcaa gtgatacaag cctggtatca
     2701 attaaaggtg aagaaagatg tagatcgaac tcgtaaggct ctcaagattg ctagacagaa
     2761 aatggatagt agtcgaatgt tgagggacga gaaacgctat tatgaaaact tgttagcaga
     2821 gctggaaaag gaaatgttag aaggaactga agatggaact agaaatttct gatttccaag
     2881 gttgcaagat tgccctcttt tgtggggata agctactgac gattttgcgt gatgacaagg
     2941 acgatatccc ttgtcccaac atgtgggagt tgccaggtgg tggccgtgaa ggggacgaaa
     3001 gcccttttga atgcgctcgg cgtgaagttt atgaggaact gggaattcat ttagatgaag
     3061 actgtctgct ttggagcaag atttatccta gcgtaatttt taaaggcaag aaatcggtct
     3121 ttatggtggg tcagctaaga caagaacaat ttgacaatat catcttcgga gatgaaggac
     3181 agggctatca gctgatgaat gttgaggaat ttcttagttc cagtcaagtt gtgcctcagt
     3241 tgcaagagag attaaaagat tatttaaaag taagtgatta gaaaggatgg ttcagttaca
     3301 tttctaaact gaacccgccc taaacactgt gccaaaaaga taaacttctc ttagacacaa
     3361 gcgtcttcag agaatttcct attttggttt tgtgttttac gggcttggta tcttaataat
     3421 ggaaacatgg caagagttaa aagttacagt gaagcgtgag ggagaggagt tggtttccaa
     3481 tctcttgatt gagctgggag cgcaaggagt cgcgattgaa gacagcatgg actatatggg
     3541 aaatgtggat cgttttggcg agattttccc agaggtggag cagcaagaag aaatcgtagt
     3601 gacagcctac taccctgaca cggtggatat gacagtggtt gaggcggact tgcaggctcg
     3661 cttagcagaa ttgacggatt ttatggattt gggagagtta aaaataggta cgactgcctt
     3721 ggctgaggaa gactgggcag acaactggaa gaaatactat gaacctgctc gcatcactca
     3781 tgatttgacc atcgttccct cttggacaga ctatgaggcg actgcgggag aaaaaattat
     3841 caagctggat cctggcatgg cttttggtac tggaacccac ccaactacca agatgagcct
     3901 ttttgccttg gaacaggttc ttcgtggtgg cgaaacggtg ctagatgtgg ggactggttc
     3961 aggggttctt tctattgcaa gctcactcct aggtgccaag gagattttcg cctatgactt
     4021 ggatgatgtg gcggttcgtg tcgctcagga aaatattgag ctcaacactg gtatggaaaa
     4081 tatccatgta gcagcaggag acttgcttaa gggagttgag attgaagcag atgtcatcgt
     4141 ggctaatatc ttggcggata ttctcattca tctgacggag gatgcttatc gcttggtaaa
     4201 ggatgaaggc tacctcatca tgagtggcat tatcaaggac aagtgggaca tggtgcgcca
     4261 gtcggctgag tcagctggat ttttccttga aacccatatg attcaagggg aatggaatgc
     4321 ctgcgtcttc aagaagacca aggatatttc aggtgtgatt ggaggctagc atgcaacagt
     4381 attttgtcaa ggggagtgca gtctctcctg tcaccatcga ggacagggaa accagcaagc
     4441 atatgtttca ggttatgcgc ttgaaagaag aggatgaggt taccttagtc tttgatgatg
     4501 gcatcaagcg cttggcgcgc gtgctggata tggaaaatcg tcagtttgag ttggtccaag
     4561 aattaaatga caatgtggaa ctaccagtcc aagtgaccat cgcatctgga tttcccaagg
     4621 gagataagtt ggagtttatc actcaaaagg tgacggaact aggtgccagc cagatttggg
     4681 ccttccctgc cgattggtcc gttgctaagt gggatggcaa gaaattgggt aaaaaggttg
     4741 aaaaactaga aaaaattgct cttggagcag cagagcaaag taagcgtaat gttgttccaa
     4801 gtatccatct tttcgagaat aaagcagact ttctagccca acttgaccag tttgaccgca
     4861 tcttagtagc ctatgaagag gcggccaaag aaggagaagc cgctgctctt ataaaagcag
     4921 ttactggact cgagaaagga gccaaattgc tctttatctt tggtccagaa ggtggtctgt
     4981 cacctgcgga aattgaaaat tttgaagcta agggagctgt tttggcagga cttggccctc
     5041 gcattctaag agcagaaaca gcaccgcttt acgccttatc agcccttagt gttttagtag
     5101 aattagagaa ataagaggaa gaaaatggaa caaaaacacc gttcagaatt tccagagaag
     5161 gaactttggg atttaacagc tctataccaa gaccgtgagg atttcttgcg tgcaatcgag
     5221 aaagctcgcg aagacatcaa ccagtttagc cgtgattaca agggcaatct tcacactttt
     5281 gaggatttcg agaaggcctt tgcggaattg gaacagatct acattcagat gagccatatt
     5341 ggcaactatg gttttatgcc tcagacgacg gactatagca atgacgaatt tgcaaatatt
     5401 gctcaagctg ggatggaatt tgaaacagat gccagcgtag ccttgacctt ttttgacgat
     5461 gccttggtgg cagcagacga ggaagtcttg gatcgtttgg gtaaattgcc acatttaaca
     5521 gctgccattc gtcaggctaa aatcaaaaaa gcccactact taggggcaga tgtggagaag
     5581 gccttgacaa atctcggtga agttttctac agtccgcagg acatttatac taagatgcga
     5641 gctggggatt ttgaaatggc tgactttgaa gcccatggca agacctacaa aaacagcttt
     5701 gtgacctatg agaatttcta ccaaaaccat gaggatgctg aggttcgtga gaaatccttc
     5761 cgttccttct cagagggact tcgtaagcac caaaatacgg ctgcagcagc ctatctggct
     5821 caggtcaagt ctgaaaaact cttggctgat atgaagggat acgactctgt ctttgactat
     5881 cttctagctg aacaagaagt ggaccgtgtc atgtttgacc gccagattga cctcatcatg
     5941 aaggactttg caccagtcgc tcagagatac ctcaagcatg ttgccaaggt aaatggtctt
     6001 gaaaagatga cctttgcaga ctggaaattg gacttggaca gcgccctgaa tcctgaagtg
     6061 actattgacg atgcctatga tttggtcatg aagtcggtag aacctttggg gcaagaatat
     6121 tgtcaggaag ttgctcgtta ccaagaagag cgctgggtgg actttgctgc taacagtggc
     6181 aaggattccg gtggttatgc ggcggaccca tatcgcgtac acccttatgt actcatgagc
     6241 tggacaggcc gtttgagcga tgtctatacc ttgattcatg aaatcgggca ttctggtcaa
     6301 ttcatctttt cagacaatca tcaaagttac ttcaatgccc atatgtcgac ctactatgtt
     6361 gaagcaccgt caaccttcaa tgaattgcta ctcagtgatt acttggagaa ccagtctaat
     6421 gacccacgtc aaaaacgctt cgctctggct catcgcttga cagacaccta cttccataac
     6481 tttatcaccc acctcttgga agccgccttc cagcgtaagg tgtatacatt gattgaagaa
     6541 ggggagacct ttggagcaag caagctcaac agcattatga aggaagtttt gacggatttc
     6601 tggggagatg ctattgaaat tgacgatgat gcaactctga cttggatgcg ccaagctcac
     6661 tactatatgg gcttgtatag ttacacttac tcagcaggac tagttatctc gactgctggt
     6721 taccttcatc taaaacattc tgaaactgga gctgaagact ggctcaatct cctcaaatca
     6781 ggtggtagca agacaccact tgagtcagcc atgattatcg gtgctgatat ttcaacagac
     6841 aaaccactcc gtgataccat ccaattcttg tctgacacag ttgaccagat tatctcctat
     6901 agtgctgagt tgggagagta ggggaataga aaaatttttt agaatgatgt ttatgggctt
     6961 gttaagcgtc attctatttg tcttagttgt agctattttc aaaaatgtcg ttgaagatcg
     7021 tgatgttaaa tttgaactta ctgtagaact ggtattgata gttatttacg tcattttata
     7081 tcagattgta tttaattgag aattcaatat tgagtcattt aagactgcct ttttgaacag
     7141 ttgctagaaa tgtagaggta ttgaccatgt atcaagattt actaagaaaa atagcagaag
     7201 aaaaaccaaa ttataaccag gaagaaatcc agtggttgtt tgatcatttg ggaaatcctt
     7261 ctccagaaat tcgtaatgtc cttttaaatc aaggcttgca ctatctttca aaagaaaagg
     7321 atacaagggg tttttcaagt cagtatggtt gggttcatgc ttttgcacat ggagccgatt
     7381 tactgacaga ggtggtttgt catccgggct ttcctaaaaa cagagttcat gaagtatttg
     7441 agatacttgg ccaattattt aaaagaatgt cgattcgctt tacagatgat gaggattggc
     7501 gtttagcaag agtgatctat gaacctattt tacaagggaa gttagcgcaa gaacaagtag
     7561 cttcttggat aaaaactgtt gattttccga tagaagaaag ggaggatttt tacaaatttt
     7621 ccaactttag atcctgtctg gtggaagtct atgtccaact tgaccagaga aatagtttac
     7681 aagatgagtt gaaagaagct atccagtctt ttcaatacta gagattagct aatagttcta
     7741 tgattttcaa aaaatttcca atcaattttc ttgaaaccct ttcctacttt tgatagacta
     7801 gtatctagtt tttgaaaaaa ggagaaagaa aatgaaaaaa tttgttgctg agttaatcgg
     7861 tacgttcatg cttgtgttcg ttggaacagg agctgttgtt tttggaaatg gtcttgatgg
     7921 ccttggtcac cttggaatcg cctttgcctt tggtttggca atcgtggtgg cagcctactc
     7981 aatcggaact gtttcaggtg ctcacttgaa cccggctgtt tcgattgcta tgtttgtaaa
     8041 caaacgtttg tcatcttcag aacttgtaaa ctacatcctt ggtcaggttg ttggagcttt
     8101 catcgcttct ggcgcagtct tcttcctctt gagtaattca ggtatgtcaa ctgctagtct
     8161 tggtgaaaat gccttggcaa acggtgtcac tgtctttggt ggtttcttgt ttgaagtcat
     8221 cgcaactttc ttgtttgtat tggttatcat gactgtgact tcagaaagca agggcaatgg
     8281 cgcgattgct ggtttggtaa tcggtttgtc attgatggcg atgattcttg tcggattgaa
     8341 gattactgga ctttcagtaa acccagctcg tagcttggca ccagctgtct tggtaggcgg
     8401 cgcagccctt caacaagttt ggattttcat ccttgcacca atcgctggtg gagttcttgc
     8461 agcccttgtt gcaaaaaatt tccttggaac agaagaataa ttgaaactca aaaagccttg
     8521 ctcctcatct tgaggaacag ggctttttcg tatgatactc ttcgaaaatc tcttcaaacc
     8581 acgtcagctt taccttgccg taggtatggt tactgactac gtcagttcta tccacaacct
     8641 caaaacagtg ttttga
//
LOCUS       DAVBCG010000084         8559 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBCG010000084 DAVBCG010000000
VERSION     DAVBCG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.084"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            221..2830
                     /locus_tag="VQ495_001522"
     CDS             221..2830
                     /locus_tag="VQ495_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2531461.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            2863..4803
                     /locus_tag="VQ495_001523"
     CDS             2863..4803
                     /locus_tag="VQ495_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2531462.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            4800..6407
                     /locus_tag="VQ495_001524"
     CDS             4800..6407
                     /locus_tag="VQ495_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531463.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            6422..7162
                     /locus_tag="VQ495_001525"
     CDS             6422..7162
                     /locus_tag="VQ495_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531464.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            7165..8484
                     /locus_tag="VQ495_001526"
     CDS             7165..8484
                     /locus_tag="VQ495_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531465.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
BASE COUNT         2949 a         1060 c         1602 g         2948 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagagatt ctctggagtt atggatgtcg ttccaatatg tgcacgttgg aatgttagtg
      121 cttatatggg gagaatccaa aggattctcc ctttttattt aaataagtct gtctagctat
      181 agatatatta agtttgataa aatttcttgg agattttgtt atggattata atttcaattt
      241 ggagcatcct ttctttttca ctaataatga ttatagtaca gatactagca taaagtacca
      301 agtaagtctg ccttttaatt ggcacgaagt aatgaataat gatgagtggg tttatcaata
      361 ccctattgga aaatttgttg aaagacaagg gtggaaaata cacattagtt cagagtataa
      421 cagttcacat gaattgttac aagatgtagc aaaaatttgc catgaaatgc gaattccatt
      481 taaacatctt agtacagaag acaaatttat catgcgaaac gggaaattgg ttagtcgagg
      541 attttctggt aaatttatta catgttatcc aaatcaaaat gaattagagt cggttttgca
      601 aaggttagaa agtgccttaa agcaatataa tgggccatat attttaagcg ataaacgttg
      661 ggatgaagca ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg atgatgaaaa
      721 gaaggttgct attgatgaat taattgttgg cgatgaagtt gttaaagatg aacgtttacc
      781 agtatttaaa attccaaaag gtatagtgcc ccctgacttt ttgaataaat ggcttgacaa
      841 gaaagataaa aaacagggag actttccctt tattatagat aatgcaatta gattttcaaa
      901 cagtggtggg atatataatg ctaggctaaa agaagatggg aaaaaaataa ttttaaaaga
      961 ggctagaccg tatacaggtc taggatttga tggtacttat tcttcagaga aattagcttc
     1021 ggagtgcaaa gcactaaaaa ttctaaatga atggagtgag acgcctaaaa tatactggca
     1081 cggtaagatc tgggagcaca ccttcttagg aattgaacat atgaagggcg ttccactaaa
     1141 tagatgggtt accaataatt ttcctttata tgaagttgtt gataagacca aagattatct
     1201 attgagagtt tcaaagatag tagaaaaact catcgattta acgaataagt ttcattctga
     1261 aaatgtatat catcaagatt tacatttagg aaatatcttg gttaaggatg aagatgagat
     1321 tagtataata gactgggaac aggctgtctt ttcaaatgat gaaaaagttg tacataaagt
     1381 tgcagcccct ggttttagag catggcgaga aacacttccc agtgagatag attggtatgg
     1441 tattagacaa atagctcatt atttatacat gccactagta actacatcgg atttgacata
     1501 caactatgta agtcaaacca ggatagaagg aaaaaaacta tttgagtctt tgggttatac
     1561 aagagaacat atagattatg tagaaagttt attgtcatat ctagattcta aatgtccaca
     1621 gattgagaat atttccagga aaaaagtttt aaaacccatg catgagatta gaactatcga
     1681 gagtgagcaa gatatacaag attttattat aaaattattg agagggttta cgctaactta
     1741 tggacagtgg cgtaaagaat ttcaatcaag attcttccca gttcattatt atggtctgaa
     1801 ttttaatcaa ggaattgcgt tttcagattt agctatcctt tggagttatc aacaactagc
     1861 taagaaagta aaaaatttca agtttgatga ctattatgag attcgaacac aggttattaa
     1921 tgaggcagta aataatttta agaaatcgtc cctttctggt ctttttgatg gtaaaattgg
     1981 tacaatctgg ctcatttatg aatttgggga gatagatcgg gcagttgaac tttttacaac
     2041 tcattttata gagatatttg agaattcaca aaataaaaat ttatatagtg ggcaggctgg
     2101 aattttactg gttggactgt attttcttag taaaggggaa attgataata aactaggaga
     2161 agaaatacta attaggctta gagaatatac tttaaattat atagaaaatc cagaaacttt
     2221 ttgtaaagtt ggcgctagtg atgttcagag taatgatcct tatgagaatt ttggtggatt
     2281 actatatggt cacgcagggg tagcttggct cttcggtgaa gcttataaat taactggtga
     2341 aagtatctat aaaaatggtc tagaattggc tgtagataaa gaattagttg cttataaagt
     2401 agatagtaat aattcattgc agtacagcca aggtcataga ttactaccat atctagcaac
     2461 tggaagtgct ggcctgcttt tacttattaa tagaaataaa gaaatcttgt caagcaaata
     2521 cttaaaatat ttaacttctt tagagagggc aactgatgta gttttttgtg tattaccagg
     2581 tttatttaac ggattttgtg gtcttgaagt tgctaataat atatatagtg atatcgatga
     2641 taacttttca ggacaaaaga aattaattga acaactttat agatatttgt gtgtaattga
     2701 agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata ttgcttcggg
     2761 atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga cgattcttcc
     2821 tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca gagtaatgaa
     2881 atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc ttcatcattt
     2941 caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg gtaccaattt
     3001 gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga agataatgac
     3061 tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa aattgtcttt
     3121 gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag gatttcatct
     3181 gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt aattggaata
     3241 aaaaaatata ctatggaaga gttatttatc attgaagata tttcagtaga aagttcgttc
     3301 tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag gccaagtaag
     3361 ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga aaacgaatgc
     3421 tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa gtcagtagac
     3481 tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt tagacactct
     3541 tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac agaaaaagca
     3601 cagtactttc ttgggctctc ttcaactgaa gaaaagaaag atcaactaat tctaagggaa
     3661 acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc tagaagagtt
     3721 cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc cgtcttttat
     3781 cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat aacatttgat
     3841 gaaaagacaa cactttatga aaattcattt tctgatcgtg ttcttctgtt tttagagagg
     3901 agaacttttt acgaaaaaat acttagtttt gatttaatag aaaatacatt aaaaactgaa
     3961 tttgaaagac ccatcataaa atcgaataat actaaatatt tttctaaggt gatttggact
     4021 aaaaaagaca gttatgatgt aagcataccg atttctttat tttggaaaag tgaagatacg
     4081 gatgagctcc caagaagaaa aaaatgtatt ctaagtgtct atggggctta tggtaagaat
     4141 gataattctg atttagatga aattatgtta tctataatag atgcaggttt tatttatgct
     4201 atagttcatg taagaggtgg aggctatttg ggtggtgaat ggtatcgctc aggaaaggcg
     4261 ttaaacaaat ggaattctat cagagatttt attgagggag taaattattt aagggaaaat
     4321 gatgtaattg acagtaagcg attaggttta ataacttcta gtgcaggtgg aataattgct
     4381 ggtgcggtgt tgaacgagga aaaaaattta ttgcaaagca ttctcttatt ttcgccattt
     4441 ataaatcctt atgatacact tcagaatcca aatgatcctc tttctaagac cgaaatagca
     4501 gagtggggag atattagaga ccctgaagta aaggcttata taaagtcata ttcgcctatg
     4561 caaaatattg aaaaggcacg agactcaaat actgttatag ttaatatttt gggtgagaaa
     4621 gacccatata ttaataataa cgaagtgata gagtggtcaa agaaattaaa ttctattgga
     4681 gttaaaagtt tgttgtattt aaacaaagca gctggtcatg gaggttttac tccatcagat
     4741 gttctcttaa tgattgatac tttaaattat ttctttgaag aagtgggaag gaataactta
     4801 tgaattattt aaaatttata aaaaagacta aacttatatt aatggggatt ttaatatttc
     4861 tatcttcgtt taatggtgtc ttgctctcag gaattattgt atatgcaggt agtttaaatc
     4921 agacttcctc attttctgac gttctaagat ttggtgctat aagtattctg ggttggtcag
     4981 caatctatat atcaaattac tatttagaag taacggaagc atcaataacg aaagatataa
     5041 atgtaaaaat taaacaagga tattttagag aacagtatct ttcttctgaa atggttaaag
     5101 attactcatc tattatttca gttttgtcaa atgatttgag attaatcgaa gaaaactatt
     5161 ttagacaaat ttttgaaata atttcttcaa tattgttgtt catcgtctct ctaagtttta
     5221 tgctttattt aaacttccta gtttcaataa tatttattgt attatcagca ttgccgatta
     5281 tagtacctgt ctttatgaag aagatgttgt ctaattcagc taatgagtac tctaacagca
     5341 atgcagagta tactcacata ataaaagaaa tttttaatgg ttttaagaca ttaaaatctt
     5401 attctgttac taaagaaata attagtttgt cggataaaaa gttggataaa ctagaagatt
     5461 ctacttttaa tttgaaacga tcagaggttc tttcaaaatt ggttgcagta ctaatttcag
     5521 gtttttgctt tctagttccc ttggttgtag gatgttattt tgtaatttat cataaaagcc
     5581 tttcttttag tgaactgata ggtattttct tagcaaacga taaagtttta gggccgatac
     5641 aatcaattgc ctattcatta aataagataa atacaaccaa agatttaagg aaaccgtttt
     5701 taaaatactt aagtggagag aagaatttta tagacgctga acatgataat aacggactgt
     5761 atacttcatc aatagatgag atacacatga aagatgttgt atattctatt acaccagaaa
     5821 ataaattaag tattgacttc tcatttaagt caccatttag ggtattatta acaggaactt
     5881 ctggtagtgg gaaaacaacg attttaaatt taattaatgg ttctttaaag ccacaaaaag
     5941 gttatgtaaa tttgttatca catgggaaaa agagttcaga ttcaatacca acagttgatc
     6001 agacaccata tatttttgac actactattc gtgagaacgt aactttattt caaaatgaat
     6061 atttttcaga tgatcagata attgaggtgt taaaaaaggt aaatctatat gaagaattag
     6121 aaaagataga tatactaaat tatcaatgtg gtgaaaatgg tagtaatttg tctggaggtc
     6181 aaaaacaaaa aatagcttta gctagagctc tgattagaaa taataaagtg tacttatttg
     6241 acgaaatatc agctaattta gataatgata attcaaattc catacatgat attctgttca
     6301 atttaggtat ttcatttatt gaagtttcac atcattatga cttaaatgac aagagataca
     6361 ctgatatata taaattggaa aatggaacac ttttcaaaat taagtgattg gaggcaagaa
     6421 tatggataaa gattatatat taaaagtgaa agggctgtat catcaatttt tactaggaaa
     6481 taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct gcttcgagag gagaatttat
     6541 aagtattcta ggaattagtg gttctggaaa gtcaacttta ttaaaatgta tttctagttt
     6601 gcttgaacca acaagtgggg aagtaatttt aaatggaatc aacccctata aaatcagaaa
     6661 tgcaaaattg tcaagtataa gacgtaacga agtatctttt atatttcaag catacaattt
     6721 aataccttcc ctgccggtaa tagaaaatat agcacttcct ttgcgattat cacaaaaaaa
     6781 attaactatt aaaaatgtag aaaacttact caaaagaatg aagtttaatg ctggcttaaa
     6841 cgattttgtt ggaactctgt ctggtggaga acaacagaag gttgctatag ctagagcggt
     6901 tattgctgat agtgatataa tatttgctga tgagccaact ggggctttag acagcgtttc
     6961 tcgtgaagta atttttgaat tattgagaga gttagtaggg gcgggtaagt gtgtaattat
     7021 ggtaacgcac gatatagaat tggcctcgaa aactgatcgt gcattaatat tgaaagatgg
     7081 aaaaattttc aaagaacttc atagacctag cggggaagag ttgtataaaa tcttagaggt
     7141 acaatcaact acggaggaat agagatgctc agattagttt actatcaatt tttacataat
     7201 aagaaacaat ggttaggcgt ttcaccagta atttttgttt cgagtttagt tatgggattg
     7261 gctgttaatg gggtaattaa tgttgagaat aattcgcaag tatttgtcgg acttccagat
     7321 cctaaaccaa tttttatgtt tccgatagta ttcggaggag taacattatt ctttgtgctc
     7381 tctaacataa taaatatgtt ggttgaaatt tttagagatg attacgaact tctggaggtg
     7441 ttgggagcaa gtctccttca actatcattt cttgtggggg gacagatttt tatcatttcc
     7501 tcgattatta gttttattgc atatctatgc tctatatttg ttacgtcaaa ttattattat
     7561 tttttacaat actttttcgg ggaaaatata ttacctgata tccaatttca aacgagtgct
     7621 gttggatgta tcattacagt agtgttgatt tcatttttag cctttctaag tggttgcttt
     7681 tatacgttca agaagattag aaataggaag agttctaaaa taaggcatgt tctttcaata
     7741 gtaaaaagaa tactgctatt ggctggtttt agtgttatct ggttgctttc tttgcaacaa
     7801 attttccaag atagcacaat tttagcgaag gcccagataa tattcaatat tgtaatctta
     7861 gatattgtga ttatttatca attatctcct tttatacagt catgttttat caaactactt
     7921 tctataatta tatttagaaa taattttatg ttcattgctt cgaagtggaa tttactatat
     7981 cgtaagcctt atataaaatc tatcagtgct gcaattacag gagctatatt actaatttct
     8041 agttttcaaa tgatttctca gaatatacta tctcaatttc aagatgacag tgatttagag
     8101 ctaaaagtgg catttattgt ctatgtagga gcaccaattt tgatagtact tgcaaatatt
     8161 atttcaattg catttttgtc atcgcatcaa gaaaggattg agattcagca atttgaaatt
     8221 ttaggaactt ctaattacca gatggttaag attaaagttg gtgaggctat atttttgaca
     8281 tttgttactt cattaattgc atttttactt aacattaaaa ttatggcttt aatatactac
     8341 tctttagagg atgttttaat agatgatatg aatcttttgg ggttaatact tccaaatttt
     8401 atagtgtcta ttattttatt tattttaata tttattacta aaagttccta ttttatcttt
     8461 aagaatgcaa aaattatttc ttaaaaattt gtaagttttc gctatataaa atagttaaat
     8521 ctattttgta tcattgcttg cattttactg gtggagaga
//
LOCUS       DAVBCG010000085         8197 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBCG010000085 DAVBCG010000000
VERSION     DAVBCG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.085"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(1..741)
                     /locus_tag="VQ495_001527"
                     /pseudo
     CDS             complement(1..741)
                     /locus_tag="VQ495_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ495_001528"
     CDS             complement(879..2501)
                     /gene="groL"
                     /locus_tag="VQ495_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2531466.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ495_001529"
     CDS             complement(2517..2801)
                     /gene="groES"
                     /locus_tag="VQ495_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2531467.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3071..3466)
                     /locus_tag="VQ495_001530"
     CDS             complement(3071..3466)
                     /locus_tag="VQ495_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2531468.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3544..4305)
                     /locus_tag="VQ495_001531"
     CDS             complement(3544..4305)
                     /locus_tag="VQ495_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2531469.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4338..4964)
                     /locus_tag="VQ495_001532"
     CDS             complement(4338..4964)
                     /locus_tag="VQ495_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2531470.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4980..5297)
                     /locus_tag="VQ495_001533"
     CDS             complement(4980..5297)
                     /locus_tag="VQ495_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2531471.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5294..5593)
                     /locus_tag="VQ495_001534"
     CDS             complement(5294..5593)
                     /locus_tag="VQ495_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2531472.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5878..6069)
                     /locus_tag="VQ495_001535"
     CDS             complement(5878..6069)
                     /locus_tag="VQ495_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2531473.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFSFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(6230..6904)
                     /locus_tag="VQ495_001536"
     CDS             complement(6230..6904)
                     /locus_tag="VQ495_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531474.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            complement(6910..7356)
                     /locus_tag="VQ495_001537"
     CDS             complement(6910..7356)
                     /locus_tag="VQ495_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2531475.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7470..7973)
                     /locus_tag="VQ495_001538"
     CDS             complement(7470..7973)
                     /locus_tag="VQ495_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2531476.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7970..>8197)
                     /locus_tag="VQ495_001539"
     CDS             complement(7970..>8197)
                     /locus_tag="VQ495_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HET2531477.1"
                     /translation="VLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPL
                     MQEIRRLDKLVDWLAKGKTSQQVLEKYEKHK"
BASE COUNT         2322 a         1904 c         1557 g         2414 t
ORIGIN      
        1 ttgatatgag aatagtcttt ctcaacatcc ttatccaaca agcatgctgt ggcgtccttc
       61 ttaacttgaa ccaggtcaat attctgggct tgggctgcca tagacgcagc cacaataaca
      121 ttgacgatag atatcatact cctcacacat ctctactgaa cgtttgtagc cttgattttt
      181 cttgaaatcg ttgggaagat agtgggtcgt gtaaattttt tgcacatcga ttccgatgct
      241 attgatggtt tgagaattct tatgaggact gatggtcaag gctgaaccaa agtagtcaaa
      301 gcccaagtcc atagccactt gcgctgtttt atccagtcgg tagtcaaaac aaaccttgca
      361 acggtcgcca ccttcgggct cctcctctag tcccctaact agttttcggt attaattggg
      421 ttcgtaggga gcttctaggt actgaaccgt atttcctgtc cgatcattaa aatcactaac
      481 aaatttcttg atgacataga cccgcttatg gtattctact ttgggatgga tattagaatt
      541 ggcaaaatag atggtcacat ctgcatactt ggtcaaatat tctagtgtat aggtactaca
      601 aggggcacag caaacatgca cgagaatggt tggccgctgc tcatttttct cccatgcttg
      661 taccattttc tgcatgacac ggtcataatt aatcttctga ttggggggca tcttgcacag
      721 aatttcttct acatcgatca tgttcttctc ctttttctag tcttatttta tcatataagt
      781 tagaagagct caaatcaatt tagaagtgaa tattataaaa agaagggtta gtcattccct
      841 ccttttgtgt tttttataag ttgttttcta tagaaagctt acatcatccc gcccatcata
      901 cttggatcca ttgctggagc tggggctact ggttctggtt tattggctac gactgcttct
      961 gttgtcaaaa tcaagctggc tacagatgct gcattttgta gggctgaacg actcacttta
     1021 actggatcaa tgataccttg atcaatcatg ttaacccact cgccagttgc tgcgttgaat
     1081 cctataccaa gctcagcatt tttcaaacga tcgataacga tagatccttc aaatcctgca
     1141 ttgtgagcaa tttgacgaac gggttcttcc aaagcacgga gaacaatatt acgtcctgtt
     1201 gcttcatctc ctgtcaattc caaggtagca acagctggaa tcacattggc aagagctgtt
     1261 ccaccacctg caacaatacc ttcttcaaca gctgcacgag tagcgttgag ggcatcttca
     1321 atgcggagtt tcatttcttt caactcagtt tcagttgcgg ctccgacctt aataaccgct
     1381 acaccacctg acaatttggc caagcgttct tgcaattttt cacggtcaaa ttcagaagtt
     1441 gtagtttcga tttgagactt gataaccgca acacggtgag aaatcgcttc aggatttcct
     1501 gcaccttcta caataaccgt gctatctttg tccacggtca ctctcgctgc ttgaccaaga
     1561 gcttcaattg tcgcatcttt caactcaaga ccaaggtctt ctgtgataac tgttccgcct
     1621 gttaagatgg cgatatcttc aagcatggct ttgcgacggt caccaaaacc aggtgccttg
     1681 actgctacta cgttgaaggt tccacgaatc ttgttcaaaa caagagttgg aagagcctcg
     1741 ccatccacat catccgcaat aatcaagagt ggacgattgc tttggagaat gctttccaaa
     1801 agtggcaaga tttcttggat attggaaatt ttcttgtctg taatcaaaat gtacggattt
     1861 tcaaggtcag ccaccatttt ttcgctatca gtcaccatgt actgtgaaag gtaaccacgg
     1921 tcaaactgca ttccttctac gacttcaagc tctgtttcca taccacgtga ctcttcgatg
     1981 gtgatgacac cgtctttgcc aactttttcc attgcttcag agatgtactc accaactttt
     2041 tcagaacgag aagatacggc tgcaacttga gcgatagctt ctttattggc aacagggatg
     2101 gcgttgtttt tcaaagcttc aactgctgcg gcaactgctg tttcaatccc acgacgaata
     2161 ccgattggat ttgcacctgc tgtgacgttt ttgattcctt cacggacgat tgcttgggtc
     2221 aaaacagttg cagttgtagt tccatcacct gcgatatcat tggtttttga agccacttct
     2281 gataccaatt tggcacccat attttcaaaa tggtcttcta attcaatttc tttggcaata
     2341 gtcacaccgt cattggtaat caatggtgag ccaaatgatt tttcaagaac gacattgcga
     2401 ccttttggtc ccaaggttac tttaacagtg tctgcaagga tatcgacacc acggaccata
     2461 gctgaacggg catctgatga aaatttaatt tcttttgaca tacttacttt ctccttctat
     2521 tcttcaatga ttgccaagat gttagcttcg cctacgatga tgtacttttc atcgccatct
     2581 ttgacatcaa gacctgcgtg ggcttcaact aagacacggt ctccagtttt aacacttgga
     2641 gcaaccaagt caccgttcaa ggtacgaaca ccttgtccag tagccacaac ttgggctgtt
     2701 ttggtttttt cttgggctga acctgcgaga acaaagcccc caacggtttg ttctttttct
     2761 tctattttta agaccacacg gtcccctaat ggtttcaaca tctgatttcc tccataatga
     2821 gatagatatt attagcgctc tttatacctg agtgctaatt catagttcta ttgtatcact
     2881 tagtcagaaa taatcaagaa aaaagtctga ctttctcaag ataaaaagcc tgagaccaac
     2941 tcagactttt taatgtttat actcaatgaa aatcaaagag caaactagga agctagccgc
     3001 aagctgctca aagcattgct ttgatgttgt ggataaagct aacgtggttt gaagagattt
     3061 tcgaagagta ttaaaatggc aattcttcct cttccaagac taaatctgcc aaatcttggc
     3121 ctgcattatt ttcacgcatg gcacgttgag cacgactttc caagagttgg aatcctgtta
     3181 caagtacttc ggtcacgtag ttcatttggc catttttctc aaagcgacgg gtacgcaatt
     3241 ctccatcaac ggaaatgaga ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc
     3301 tgccccatag gaccatattg acaaaatcag cttcacgttc accgttttgg tctttgtaac
     3361 gacggttcac agcgatagtt gctcgcgcta ccgacttgtc attgttggtt ttgtgcaatt
     3421 ctggtgtaga cgttaaacgc ccaatcatga taactttatt atacatattt tcttcctcct
     3481 acttatctat tcgtaggaaa tcaaaaaaag ttacagaaat ttgtaacttt tcgaggaaat
     3541 ttcttatttt ttatgaatca tgaaacctgt cgcctgttga ttggccataa tggtcatatc
     3601 tgtaatctga acacggcgag gctgactggt cacatagact actgtgtctg caatatcctg
     3661 agcttgcaag gcttctattc cttggtaaac ggacgcagct cgctctttat caccatgaaa
     3721 acgaacagtt gagaaatctg tttcgacaat cccaggctga atggttgtca ccttgatatc
     3781 cgttgcgatg gtatcaattc gcagtccatc cgaaaaggtc ttaaccgcag ccttggtagc
     3841 tgagtaaaca gcggcaccag cataggcgta gattcctgcg gttgacccca tattgataat
     3901 atgaccttga ttgactttta tcattgctgg caagaaacag cgagtaaccg ccatcagacc
     3961 tttaacattg gtatccaaca tggtcaacat atccaactcc tcatagtctt gatagggagc
     4021 caagtcaaga gccagtccgg cgttgttgac caagatatca atcctcccta tcgtttctag
     4081 aatatcagag caaacagtct tcaccatgac catatccgtc acatctagtg gaaaagtcca
     4141 gacggtttga tttggaaagc ttactgcaaa ctccgacttg aggatttcta atctgtctat
     4201 ccgtcgtcct gttagaacga catcctcacc ctgctccaga taagcacgcg caatcgcttc
     4261 accgattcct gaggttgctc ccgtaatcac tacatttttt gccatcttat ttccttctat
     4321 cagatgctga caagttccta ggcagtccag tgtttcgctg ggtcgaacgg tgttccgaca
     4381 acttggtctt ctgataattc aagcacccca cgtttttgcg gagcatttgg caaatgcaat
     4441 tcacgaggac tacacatcat cccaaaactc ttttcaccac gaagttcgcc tgggaaaatg
     4501 aaattccctt ttggcatcat ggcaccagga agagccacaa tggttttcaa cccaacacgc
     4561 gcattaggtg ctcctgcaac gatttgcact atcttgtcac ttgcgactgc aacttggcag
     4621 atgttgaggt ggtcactgtc tggatgagct accatctcga caatttcacc aacaacaaac
     4681 ttaggttcct tatcattaac aatttcttct gtaaaacctt ccgcctgtaa ttcttggttc
     4741 aaacgagcga cttgctcatc tgataaaaag acttgaccgc gctctgcaat ttcaaacaag
     4801 ctcgaaacct caaaaatgtt ccaagccact gtttccccat tttctttgag gaaaacacgg
     4861 gctaccttgc ctttgcgttc cacattcagt ttggcatctc cgctattttt cacgatgacc
     4921 ataaggacat cactgacatg ttctttgtta tacgtaaaaa tcattgtttc ctttttctcc
     4981 tatttcaatc ctgctaaaaa atcgttaatt tgctccttac ttttacggtc gcgattgaca
     5041 aaacgaccga tttccttgtc cttttctaga acaacaaggc taggaattcc gtaaacatcc
     5101 cagagtttgg ccaaatccat atactgatct cggtccattc gaataaaggt gaactctgga
     5161 ttggtctctt caatctctgg taaggcagga taaatataac gacaatcgcc acaccagtct
     5221 gccacaaaaa ggaagacctt cttgcccgct ttttccacta aagatgctaa ttcttctaaa
     5281 cttgctggct gtatcataag acttcctcct catagactag gtcttcattt tcatagacaa
     5341 aggtataatg acggccatcc tcaaaaatga cgccaccaac caagctctcc agactgcttt
     5401 cgtaaacttg aacataaagg gtcgcaattt cccccatgtc ggaaaaatgg tctcgcacaa
     5461 tctctgtcaa ctcttcctga gtcttcatga gcttacggtc atctgcaact tttttcgtag
     5521 caagagcaag gcttccgata cctagcagag ccaagcctgc catccacatt tttttagctt
     5581 tcataccatt cattttaaca caaaaaaggc ttcaggacaa atgaggaagc agcagaaaag
     5641 caagtaaaaa gcctcttcct ttaaggaaaa ggacttctta tactaagagc aaactagaaa
     5701 gctagccgca ggctgctcaa aacacggttt tgaggttgta gataaggcga cgttgacgtg
     5761 gtttgaagag attttcaaag agtattattc ttattgccag gcacctaagt tgccaacgta
     5821 gtaaccatca ggtgtgtagg tattgcgaag catcttacct gatgaagcca gataatacta
     5881 cttgccattg tctttgaccc aatcattcgc aatcatggaa ccagaagaac ttacataata
     5941 ccattctccc ttgtcataaa cccaagtact gactttcatg gttcctgagc aattaaagga
     6001 aaaaaaactg tccaatatca ttcgtttttt aaaagcattc gacactacat catctacgct
     6061 gtagtacata gatacatcaa acgtaaaagc caaaatatcg aaaaatatac aacttttaaa
     6121 ggcagcattt cattttttaa agtctaacaa ccatagaact gaaatcagag caaggcatca
     6181 atttcctact cttagtacgc ctattttgat ggttgaagtg aagatttttt tatttagcgt
     6241 agacaatttc caacttccca aaagccacat ctccacggat aatcaaggtt ttgctggttg
     6301 gggctacagg agcgtcagcc ttagctgcac caaaggaggt ttctactttc aaatctacac
     6361 gccagtgttg tggaacatag acggttgcat tcccgaaggc cacttcaata tttaaagttg
     6421 caaaatcacc tagcatctct gcattatcat agtagatttt agcatcccca aaagcgactt
     6481 ccacttggtc atctacgaga tcttgatctt gcttatagaa ggtcccgcta ccaaaagcga
     6541 cttccttatc cgtgacgact gtttttttcc cattgtacca ccattttttt tcattccaga
     6601 acttacttga atgcgtcaga taaccaacac caagtaccac caagatgcta gcccaaataa
     6661 gagaatgatt ggtaactggt aacaagtcat aagcgtaatt tgcaatgatg agcgccagta
     6721 aacctgtaaa aactgccgaa gtgagatgac gtctaaggat ggactcaatg gacttataag
     6781 caaaaaaaac aatacctagt aaaggccata ttttaccatc caaagaagga attccaaaat
     6841 tcccttgcag caagatccaa gctgctaaaa ccaataaaac aataccaaat gctttctttt
     6901 tcatgttttt tacctcatgt ttcttagatt ttcttttagg agggattggt aatgccgtga
     6961 gacatgaacc tccttgtgcg tctgataaaa ggaaatggtg cccgttcctg aaaaggactt
     7021 gtccactgag taaatctgac ggatgtttgc gatcgttgac ttggaaactc gattaaaata
     7081 gcgaggcaag atagactcca actcatagag cttgaggcga acctcataag cttccttctg
     7141 gttatgagcg tagatcttgc tcccttctgt ttcaaagaag agaatttctg acaggtctag
     7201 ataatattca cctgtcccct tgtaaaaagt caacctcgga gcctttgact cttgcaagag
     7261 ccgttgcaag tctgcaattt catctgtcaa agtgggggtc ttgatgacaa tttcagtctc
     7321 ctctaaattg ccgtcaatct caattcgtaa cttcatcaca tctcctttct gactctacta
     7381 tatcaaagct aagttaggaa tacaagggca aaaaagcaag tggtcaattt gaagcagtaa
     7441 gtggttgtag gactagctta acttacatgt cagaatagga agaaaaggaa tccccacacc
     7501 aaaccacaag catagccaac caagacatcc ttgggataat gaacaccccc taaaaccctc
     7561 accaacccca gcaaagctga aaagatcaag catactaagc caataggtag actagcatgc
     7621 agacaggcca tggagatgat agttgccgaa aagacatggc gactgggcat cgaccgtccc
     7681 gaactatcct tatcaagaag gggacagata tcccaagttt cataaggtct cgggtgattg
     7741 attttcttac gaaacaggga caaggccaca aaacctgatg caggaataag cacataaacc
     7801 ccaacttgct gaccaagtcc tagttgcagg taagtggtga ctagcaagat cagatagacc
     7861 aggggcatgg cgactgtcat caaacgattg aaactgcgta ataggaataa aagaattgga
     7921 cgactagtta gtctggagct gatatttcga taccattctt gataattttt catttatgct
     7981 tctcatactt ttctagaact tgttgagatg ttttcccctt ggcaagccaa tccactagct
     8041 tgtccaagcg acgtatttcc tgcatcagag gatcatcaat ttcctcaata cggacaccgc
     8101 aaatcttccc tgtaattgca attcgttcag ggacataata tggtgcttgt ctaaaaaaat
     8161 ctccgtaagt aacatcccta tccagacaag caagaac
//
LOCUS       DAVBCG010000086         8094 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBCG010000086 DAVBCG010000000
VERSION     DAVBCG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.086"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            76..1701
                     /gene="treC"
                     /locus_tag="VQ495_001540"
     CDS             76..1701
                     /gene="treC"
                     /locus_tag="VQ495_001540"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2531478.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            1843..2091
                     /locus_tag="VQ495_001541"
     CDS             1843..2091
                     /locus_tag="VQ495_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2531479.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            2298..3092
                     /gene="racE"
                     /locus_tag="VQ495_001542"
     CDS             2298..3092
                     /gene="racE"
                     /locus_tag="VQ495_001542"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2531480.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            3089..4060
                     /locus_tag="VQ495_001543"
     CDS             3089..4060
                     /locus_tag="VQ495_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2531481.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            4036..4557
                     /locus_tag="VQ495_001544"
     CDS             4036..4557
                     /locus_tag="VQ495_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2531482.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            4554..5015
                     /locus_tag="VQ495_001545"
     CDS             4554..5015
                     /locus_tag="VQ495_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2531483.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            5006..5746
                     /gene="xerD"
                     /locus_tag="VQ495_001546"
     CDS             5006..5746
                     /gene="xerD"
                     /locus_tag="VQ495_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2531484.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            5746..6474
                     /locus_tag="VQ495_001547"
     CDS             5746..6474
                     /locus_tag="VQ495_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2531485.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            6493..7062
                     /locus_tag="VQ495_001548"
     CDS             6493..7062
                     /locus_tag="VQ495_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2531486.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            7049..7771
                     /locus_tag="VQ495_001549"
     CDS             7049..7771
                     /locus_tag="VQ495_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2531487.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            7771..8013
                     /gene="yidD"
                     /locus_tag="VQ495_001550"
     CDS             7771..8013
                     /gene="yidD"
                     /locus_tag="VQ495_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2531488.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
BASE COUNT         2346 a         1609 c         1938 g         2201 t
ORIGIN      
        1 cattgagtat aattgaaata gcacaaatag ggacggaaga ctgcttcctc ccttttatca
       61 gaaaaggaga aacagatgac acttgataaa ggaaaagtag tctatcaaat ctatccaaaa
      121 tcttacaaag acaccactgg caatggtatt ggggatttcc gtgggattat cggaaaaatt
      181 ccctatctag ccaaactagg tgtggatatg gtctggctca atccattcta tccaagtcct
      241 caacgggata atggttacga tatttcagat tatatggcag tggatcctct ttttggtgat
      301 atggctgatt ttgaggaaat ggtgtgtatc ggtaaagagc acaagattga ctttatgctg
      361 gacatggtac ttaatcattg ttcgacagag catgaatggt ttcagaaagc cctagctggt
      421 gataagtatt atcaagactt tttcttcatc caagaccaac caacagattg gcagtctaag
      481 tttggtggtt ctgcatgggc gcctttcggg gatactggga aatactatct tcacctattt
      541 gatgagaccc aggctgacct taactggcgc aatcccaatg tccgtaagga gcttttcaag
      601 gttgttaatt tctggcgcga caagggtgtc aaaggttttc gatttgatgt gatcaatttg
      661 attggtaagg acgaggtttc agtggactgt cctgaaaatg aagggaagcc agcttataca
      721 gacaaaccca tcgttcataa ctatttgcgt atgatgaacc aagccacttt tggatctgac
      781 gatagcttta tgacagttgg ggaaatgtct tctaccacca tggaaaactg tgtcctctat
      841 tcgtcgcctg atcgtcagga attatccatg acctttaatt ttcatcacct caaggtggac
      901 tataaggatg gacaaaagtg gaccttggct ccctttgatt ttgaagagtt gaagagtctt
      961 taccatagtt ggggtaagga aatgagtgat aaagacggtt ggagtgccct cttttggaac
     1021 aatcacgatc aaccacgtgc cttgaaccgt tttgtcgata ttcaaaactt ccgcaaggaa
     1081 ggagccacca tgctagcagc cagcattcac ctgtcacgtg ggacacctta tatctacatg
     1141 ggtgaggaaa tcgggatgat tgacccagac tatgattcta tggctgatta cgtggatgtc
     1201 gaatcgctta atgcctatca gatgctccta gaagaaggaa agagtcagca agaagccttc
     1261 cagattattc aagctaagtc gcgtgataat tcacgcattc ccatgcagtg ggatgcttcg
     1321 gaaaatgcag gattttcaac aggcactcct tggttgaaag ctggtaaatc ctataaagac
     1381 atcaatgtag aaaatgaaat ccagggtccg atttttacct tctatcaaga cttgattcga
     1441 ctgcgtaagg aaatgccaat catttcagaa ggaagttaca aaccagcctt tgaagatagc
     1501 aaacaagtct acgcttttga acgccagttt gaggatcaaa agttactagt gctcaataac
     1561 ttttatgcta aagaagtgga aatcgacttg ccagcagtct accaaaatgg acagattttg
     1621 atttcaaact atgaagatgc agaagtgtct gagaaaattc tactaaaacc ctatcaaaca
     1681 ctggctattt atgtaaatta aacttagcaa ggagacctct ttttcagaaa agcagggttc
     1741 tttttgctgt ctattccatc cctgaaagtg tagaacaaac tggtcagggt ggctagtttg
     1801 tggtataatg aaagggactc aaaaagaaac aggagaaaaa tgatggattt acttttagca
     1861 attgtattga ttgtgctagc ttttctagga ggagctcttg gaggaatgta cttggttcgt
     1921 aagcaaatcg aaaaagaatt tgctgacaac ccacgtttga atgctgaagc agttcgtact
     1981 cttttgagtg caaatggtca aaaaccaagc gaagctaagg tacaacaagt ttaccaccaa
     2041 atcatccgcc aacaaaaggc agcccttgct aacaataaaa agaaaaaata aataaggaaa
     2101 agtctgggat gaaagttcca gacttctcac tatgttggtt atggtttagg gatgagactt
     2161 tttccttgca ttctattgat atttgattat gattaagaga gaggtagttg acattctatc
     2221 aagctgtcgg tcagttcgtt cagtacaagg aatcataatg aacgatcaat cagaaaaaag
     2281 actagaaaga agactgtatg gataatcgac caattggttt tttggattcg ggtgtcgggg
     2341 gcttgaccgt tgtgcgcgag ctcatgcgcc agcttcccca tgaagaaatc gtctatattg
     2401 gagattcggc gcgggcgccc tatggccccc gtccagctga gcaaattcgt gaatatactt
     2461 ggcagctggt caactttctc ttgaccaagg atgtcaaaat gattgtcatt gcttgtaaca
     2521 ctgcgactgc ggtcgtctgg gaagaaatca aggctcaact agatattcct gtcttgggtg
     2581 taattttgcc aggagcttcg gcagccatca agtccagtca aggtgggaaa atcggagtga
     2641 ttggaacgcc catgacggta caatcagaca tataccgtca gaaaatccat gatctggatc
     2701 ccgacttaca ggtggagagc ttggcctgtc ccaagtttgc tcccttggtt gagtcaggtg
     2761 ccctgtcaac cagtgttacc aagaaggtgg tctatgaaac cctgcgtccc ttggttggaa
     2821 aggtggatag cctgattttg ggctgtactc attatccact ccttcgccct attatccaaa
     2881 atgtcatggg gccaaaggtt cagctcatcg atagtggggc agagtgcgta cgggatatct
     2941 cagtcttact caattatttt gaaatcaatc gtggtcgcga tgctggacca ctccatcacc
     3001 gtttttacac aacagccagt agccaaagtt ttgcacaaat tggtgaagaa tggctggaaa
     3061 aagagattta tgtggagcat gtagaattat gacaaataaa atttatgaat ataaggatga
     3121 ccaggattgg tatgttgggt cttatagtat ttttggtggc gttaacagtt tgagcgacta
     3181 taagacagat tttcctctgt ttgaattctc caaaatattt ggagatgaag agtatggttt
     3241 cccgctttca gttactgttt tacgctatgg ttctacctac cgtttgttct cctttgtggt
     3301 agacatgctt aatcaagaaa tgggacgaaa cttggaagtt attcaacgtc atggggccct
     3361 gctcttggtt gaaaatgggc aactcttgta tgtagaattg cctaaagaag gggtcaatgt
     3421 tcatgatttc tttgagacaa gcaaggtcag agaaaccttg ttgattgcga ctcgtaacga
     3481 aggcaaaacc aaggaattcc gagctatctt tgataagtta ggctacgatg tggaaaatct
     3541 taatgactac cctgacctgc ctgaagtagc agaaacaggt atgacctttg aagaaaatgc
     3601 ccgccttaag gcagaaacca tttctcaatt aacgggcaag atggttttgg cagatgattc
     3661 tggtctcaaa gtcgatgtcc ttggtggctt accaggcgtc tggtcagctc gtttcgcagg
     3721 tgtgggagca actgaccgtg aaaataatgc caaactcttg cacgaattgg ccatggtctt
     3781 tgaactcaag gaccgctcgg ctcagttcca cacaacccta gtcgtagcca gcccaaataa
     3841 ggaaagttta gttgttgaag cagactggtc aggttatatt aactttgaac ctaagggtga
     3901 aaatggcttt ggctatgatc ccctcttcct tgtaggagag acaggtaagt catcagctga
     3961 attaaccctg gaagaaaaaa atagtcaatc tcaccgtgcc ttagccgtta agaaactttt
     4021 ggaggtattt ccatcatggc aaagcaaacc atcattgtaa tgagcgattc ccatggcgat
     4081 agcttgattg tggaagaagt ccgtgatcgc tatgtgggca aagtcgatgc cgtttttcat
     4141 aacggcgatt ctgaactacg tccggattct ccactttggg agggcatccg cgttgttaaa
     4201 ggaaacatgg acttctacgc cggctaccca gaacgtctgg tgactgagct tggttcgacc
     4261 aagattatcc aaactcatgg tcacttgttt gacatcaatt tcaactttca aaagttggac
     4321 tactgggctc aggaggaaga ggccgctatc tgcctctatg gtcacttgca tgtgccaagt
     4381 gcttggttgg aaggcaagat cctctttcta aatccaggtt ctatcagtca accacgaggt
     4441 accatcagag aatgtctcta tgctcgtgtg gagattgatg atagttactt caaagtggac
     4501 tttttgacac gagatcacga ggtgtatcca ggtttgtcca aggagtttag ccgatgattg
     4561 ccaaggagtt tgagactttc ttgttggggc aggaggaaac ttttttgacc cctgctaaaa
     4621 atctagctgt gttgattgat acccacaatg cggatcatgc gaccctcttg ctcagtcaga
     4681 tgacctatac ccgtgttccc gttgtgacag atgaaaaaca gtttgttggg acgattggac
     4741 tcagagatat tatggcttat cagatggagc atgacttgag ccaagaaatc atggcggata
     4801 cggatatcgt tcatatgaca aaaacggacg tagcggttgt ttcgcctgat ttcaccatta
     4861 cggaggtctt gcacaagcta gtagatgagt ccttcttacc ggttgtggat gcagagggta
     4921 ttttccaagg gattattacg cgtaagtcca tcctcaaggc cgttaatgcc ctcttgcatg
     4981 actttagtaa ggaatatgag attcgatgcc aatgagagac aggatttcag cctttttaga
     5041 ggaaaagcag ggcttgtctg tcaattccaa gcagtcctat aagtatgatt tggagcaatt
     5101 tttagacatg gtaggtgagc ggatttctga gaccagtctc aagatttacc aagcccagct
     5161 agccaatcta aaaatcagcg cccagaagcg aaagatttcg gcctgtaacc aatttctata
     5221 ctttctctat caaaaaggag aggtggacag cttttaccgc ttggaattag ccaaacaagc
     5281 tgaaaagaag acggaaaagc cagagattct atacctagac tctttttggc aggaaagcga
     5341 ccatccagag ggccgcttgc tagcgctctt aatcctagaa atggggctct tgcccagtga
     5401 gattttagcc atcaaggttg cggacatcaa tctggatttt caggtgttgc gaatcagcaa
     5461 ggcttcccaa cagaggattg tcaccattcc cacggccttg ctttcagaat tggaaccctt
     5521 gatggggcag acctatcttt ttgaaagagg agagaaaccc tattctcgtc agtgggcctt
     5581 tcgtcagtta gaatcttttg tcaaggagaa aggttttcca tccttatcag ctcaagtctt
     5641 acgtgaacag tttattctaa gacaaataga aaacaaggtc gatttgtacg aaattgcaaa
     5701 aaaattagga ttaaaaacag tcctgacctt agaaaaatat agataatgga tattaaatta
     5761 aaagattttg aaggccccct ggacttgctc ttgcatctgg tttctaagta ccagatggat
     5821 atctacgatg tgcccattac ggaagtcatc gaacagtatc tagcctatgt ttcaaccctg
     5881 caggccatgc gtctggaagt gacgggtgag tacatggtca tggctagtca gctcatgctg
     5941 attaagagtc gtaaactcct tccgaaggta gcagaagtga cagacttggg ggatgacctg
     6001 gagcaggacc tcctctctca aatcgaagaa tatcgcaagt tcaagctctt gggtgagcac
     6061 ttggaagcca agcaccaaga acgggcccag tattattcca aagcgccgac agagttgatt
     6121 tacgaagatg cggagcttgt gcatgacaag acgaccattg acctcttttt ggctttttca
     6181 aatatcctag ccaagaaaaa agaggagttt gcacaaaatc acacgactat cttgcgggat
     6241 gagtataaga ttgaggacat gatgattatc gtgaaagagt ccttgattgg acgagatcaa
     6301 ttgcgcttgc aggatttgtt caaggaagcc cagaatgtcc aagaggtcat caccctcttt
     6361 ttggcaaccc tagagttaat caaaacccag gagctgatcc tcgtgcaaga ggagagtttt
     6421 ggagatatct atctcatgga aaagaaggaa gaaagtcaag tgcctcaaag ctagacttga
     6481 tagagaggaa agatgagtac tttagcaaaa atagaagcgc tcttgtttgt agcgggtgaa
     6541 gatgggattc gggtccgcca gttagctgaa ctcctctctc tgccaccgac aggcatccag
     6601 cagagtttag gaaaattagc ccagaagtat gaaaaggacc cagattccag tttggctttg
     6661 attgagacaa gtggtgctta tagattggtg accaagtctc aatttgcaga gattttgaag
     6721 gaatactcta aggcgcctat caaccagagc ttgtctcggg ctgcccttga gaccttgtcc
     6781 attattgcct acaaacagcc gattacgcgg atagaaattg atgccatccg tggagttaac
     6841 tcgagtggag ccttggcaaa gttgcaggct tttgacctga taaaggaaga cgggaaaaag
     6901 gaagtattgg ggcgtcccaa cctctatgtg actacggatt atttcctaga ttacatgggg
     6961 ataaaccatt tagaagaatt accagtgatt gatgagcttg agattcaagc ccaagaaagc
     7021 caattatttg gtgaaaggat agaagaagat gagaatcaat aagtatattg cccacgcagg
     7081 tgtggccagt aggagaaaag cagaagagct gattaagcaa ggcttggtga cggttaacgg
     7141 ccaagtggtg cgtgaactag caaccactat caagtcaggc gacaaggtcg aagttgaagg
     7201 tcaacctatc tacaacgaag aaaaggtcta ctatctgctt aacaaaccac gcggtgtgat
     7261 ttccagtgtg acagatgata agggtcgcaa gacggttgtc gacctcttgc ccaatgtcaa
     7321 agagcgtatt taccctgtgg gtcgtttgga ctgggataca tcaggtgtct tgattttgac
     7381 caatgatggg gactttacag acgagatgat tcaccctcgt aatgagattg acaaggttta
     7441 tgtcgcgcgt gttaagggtg tggccaataa ggacaatctc cgccccttga cccgtggtct
     7501 tgagattgat ggtaagaaaa ccaagccagc tgtttatgaa attctcaaag tggacccagt
     7561 caaaaatcgc tctgtggtgc agttgaccat ccatgaaggg cgtaaccatc aggttaaaaa
     7621 gatgtttgaa gctgttggtc tccaagtaga taagttgtct cggactcgtt tcggacacct
     7681 agacttgaca ggactccgtc caggagaatc ccgtcgtctt aataaaaaag aaatcagcca
     7741 actacacacc atggctgtaa ctaagaaata atgaaacgaa ttttaatagc gcctgtgcgc
     7801 ttttaccaac gttttatctc accagtcttt ccaccctctt gtcgctttga gctgacttgt
     7861 tccaactaca tgattcaggc tattgaaaaa catggtttta agggggtatt gatgggcttg
     7921 gctcggattt tacgttgtca tccctggtcg aaaacaggta aggaccccgt tccagaccac
     7981 ttttccctta aacgaaatca agaaggggaa tgaggtgggg taaatagatt tcaaaatgat
     8041 aaaaacgcat cctatcaggt ttgagtgaac ttgataggat gcgttttaga atgt
//
LOCUS       DAVBCG010000087         7935 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBCG010000087 DAVBCG010000000
VERSION     DAVBCG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.087"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            16..1965
                     /gene="mutL"
                     /locus_tag="VQ495_001551"
     CDS             16..1965
                     /gene="mutL"
                     /locus_tag="VQ495_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2531489.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(2290..2757)
                     /locus_tag="VQ495_001552"
     CDS             complement(2290..2757)
                     /locus_tag="VQ495_001552"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2531490.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(2758..3963)
                     /locus_tag="VQ495_001553"
     CDS             complement(2758..3963)
                     /locus_tag="VQ495_001553"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2531491.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(3983..4618)
                     /gene="ribE"
                     /locus_tag="VQ495_001554"
     CDS             complement(3983..4618)
                     /gene="ribE"
                     /locus_tag="VQ495_001554"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2531492.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(4603..5703)
                     /gene="ribD"
                     /locus_tag="VQ495_001555"
     CDS             complement(4603..5703)
                     /gene="ribD"
                     /locus_tag="VQ495_001555"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2531493.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(5838..5952)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6109..6702
                     /gene="ruvA"
                     /locus_tag="VQ495_001556"
     CDS             6109..6702
                     /gene="ruvA"
                     /locus_tag="VQ495_001556"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2531494.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            6712..7275
                     /locus_tag="VQ495_001557"
     CDS             6712..7275
                     /locus_tag="VQ495_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2531495.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            7272..>7935
                     /locus_tag="VQ495_001558"
     CDS             7272..>7935
                     /locus_tag="VQ495_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2531496.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAI"
BASE COUNT         2254 a         1594 c         1538 g         2549 t
ORIGIN      
        1 gaaaggggct gaaagatgtc tcatattatt gaattgccag agatgctggc aaaccaaatc
       61 gcagctggag aggtcattga acgtcctgcc agcgtggtca aagaattggt agaaaatgcc
      121 attgacgcgg gctctagtca gattatcatt gagattgagg aagctggtct caagaaaatc
      181 caaatcacag ataacggtta tggaattgcc cacgatgagg tggagttggc cctgcgtcgc
      241 catgcgacca gtaagataaa aaatcaagca gatctctttc ggattcggac gcttggtttt
      301 cgtggtgaag ccttgccttc tattgcgtct gttagtgtct tgactctgtt aacggcggtg
      361 gatggtgcta gtcatggaac caagttagtc gcgcgtgggg gtgaagttga ggaagtcatc
      421 ccagcgacta gtcctgtggg aaccaaggtt tgtgtggagg atctcttttt caacacgcct
      481 gcccgtctca agtatatgaa gagccagcaa gcggagttgt ctcatatcat tgatattgtc
      541 aaccgtctgg gcttggccca tcctgagatt tcttttagct tgattagtga tggcaaggaa
      601 atgacgcgga cagcagggac tggtcaattg cgccaagcaa tcgcagggat ttacggtttg
      661 gtcagtgcca agaagatgat tgaaattgag aactctgacc tagatttcga aatttcaggt
      721 tttgtgtcct tgcctgagtt gactcgggct aaccgcaatt atatcagcct cttcatcaat
      781 ggtcgttata tcaagaactt cctgctcaat cgtgctattt tggatggttt cggaagcaag
      841 cttatggttg gacgttttcc actggctgtc attcacatcc atatcgaccc ttatctagcg
      901 gatgtcaatg tgcatccaac taagcaagag gtgcggattt ccaaggaaaa agaactgatg
      961 accctggttt cagaagctat tgcaaatagt ctcaaggaac aaaccttgat tccagatgcc
     1021 ttggaaaatc ttgccaaatc gactgtgcgc aatcgtgaga aggtggagca aactattctc
     1081 ccactcaaag aaaatacgct ctactatgag aaaactgagc cgtcaagacc tagtcaaact
     1141 gaagtagctg attatcaggt agaattgact gatgaagggc aggatttgac cctgtttgcc
     1201 aaggaaacct tggaccgatt gaccaagcca gcaaaactgc attttgcaga gagaaagcct
     1261 gctaactacg accagctaga ccatccagag ttagatcttg ctagcatcga taaggcttat
     1321 gacaaactgg agcgagaaga agcatccagc ttcccagagt tggagttttt cggacaaatg
     1381 cacgggactt atctctttgc ccaagggcga gatggacttt acatcataga tcagcacgct
     1441 gctcaggaac gggtcaagta cgaggagtac cgtgaaagca ttggcaatgt tgaccagagc
     1501 cagcagcaac tcctagtgcc ctatatcttt gaatttcctg cggatgatgc cctgcgtctc
     1561 aaggaaagaa tgcctctctt agaggaagtg ggcgtctttc tagcagagta cggagaaaat
     1621 caatttattc tacgtgaaca tcctatttgg atggcagaag aagagattga atcaggcatc
     1681 tatgagatgt gcgacatgct ccttttgacc aaggaagttt ctatcaagaa ataccgagca
     1741 gagctggcta tcatgatgtc ttgcaagcga tctatcaagg ccaatcatcg tattgatgat
     1801 cattcagcta gacaactcct ctatcagctt tctcaatgtg acaatcccta taactgtcct
     1861 cacggacgtc ctgttttggt gcattttacc aagtcggata tggaaaagat gttccgacgt
     1921 attcaggaaa atcacaccag tctccgtgag ttggggaaat attaaaagta taaaaaagtc
     1981 tgggaaaaat tttcaaaatc agaaaaacgc ataaaatcag gtgttcaaaa accttgattt
     2041 tatgcgtttt atcatggaaa tagttacttc atgttttcct aattcttttc gaaactcttt
     2101 ttaaaccacg tcagttttat cagtaatctc aaaacagtgt tttgagctaa ttttgccagt
     2161 tttatctgta acatcgaagt tgtgtttgac cactctgcga ctggtttcct agtttgctct
     2221 atgattttca cagagtatta aattgcgatt ttgccaagtt tctttattcg tctaaaagta
     2281 gagtctgttc tatgcgtcta atgtacgaat taggttgacc atttcaatag ctccttgtgc
     2341 acactcagaa cccttatttc ctgctttggt accagctcgt tctatggctt gttcaattgt
     2401 atctgtcgtt agcacaccaa acataacagg aatttcgcta tttaaactga tttgggcgat
     2461 tcccttagat acctcgctac atacataatc ataatgactt gtattccctc taatgacagc
     2521 tcccaagcag ataattgcat catatttttt actttttgcc atttttgatg caatcagtgg
     2581 tatttcaaaa gctcctggaa cccaggctac ctctatatct ttctcgttta cattctctct
     2641 tttgagatta tctagtgctc cagataataa ttttgaagtt ataaattcat taaatctcgc
     2701 tacaacaata cctattttaa tattgtttgc tactaaatta ccttcataag tgttcattta
     2761 tttttcctcc atatttaaaa tgtgacccat tcgatttttc tttgtttcta aataaaaact
     2821 atcgtaagga ttggcttcta tttcgattga tattctactg gaaatggtaa ttccatattt
     2881 ttctaactgt tcaaccttgt caggattatt tgtcagtaaa tgaagtgact gaagtcccag
     2941 atctttaagc atttgtgctc caatatgata ttctcttaaa tcaccttcaa agcctaatgc
     3001 aagattggca tcaagcgtat ccatgccttg atcttgtaaa tgataggctt ttaatttatt
     3061 gataagtcca attcctcgtc cctcctgtcg caagtaaagt aagacacccg aaccattctc
     3121 aacaatcatt ttcatagctt tatcgaattg ctgtccacaa tcgcaacgta aagagcctaa
     3181 aacatctcct gttaaacatt cggagtggac ccgacataat acattggctt catcctctat
     3241 atttcccata ataagagcaa gatgatgttc cccatttagt ttatctatat agctaattgc
     3301 tttgaaatta ccgtatctag taggcatatt gacagttgaa actcgttcta ccagctgatc
     3361 atatactttt ctatattctt gtaattcttt gatggtaatt agtggaatgt tgtgtttctt
     3421 cgagaactga attaaatcat ctgttctcat cattttgcca tcatgattca ttatttcaca
     3481 acataggcca cactctttta gtccagctaa ttttaataaa tcaacagttg cttctgtgtg
     3541 tccatttctt tctagaacac cacctttttt tgcaattaaa ggaaacatgt gtcctggcct
     3601 gcgaaaatca gagggtgtta tatcttcagc tacacacata cgtgcggtca gtcctctctc
     3661 ctcggcagaa atacctgtgg tcgtttcttt ataatcaatt gaaactgtaa aagcagtctt
     3721 atgattatct gtattgtttt caaccatagg tgaaagcatt aattgattag ctaaactttc
     3781 gctcataggc atacaaatta atcctttggc ataagtagcc ataaaattaa cattttctgt
     3841 tgtagctgct tgtgcagaac aaattaagtc tccttcattt tctctatcct tgtcgtctat
     3901 aacaagaaca agtcgtccct tctgcaatgc ttctaatgct tcttgtattt ttcgatattc
     3961 cattgactga ttatcctttc tgctaaaatc cattttgata taatagttcc ttagatattt
     4021 ctgattttgg agagttatcc atcagttttt gcacatattt acctaagata tcattttcaa
     4081 gatttactgt actcccgact tgtttactct taagaatggt ttgttccaag gtatgaggga
     4141 taacagatac tgaaaagttt actttggaga ctttagcgac agtcagacta atgccgtcaa
     4201 ttgtaataga tcctttttca actattaaat ctaaaatttc tttttgtgtg ttgatttgat
     4261 accatacagc attatcatct ttttttattg acgagatttt tcctgtacca tcaatgtgtc
     4321 ctgtaacgac gtgaccccca agtcgaccgt tgacagataa ggctctttct agattcacct
     4381 cacttccatg ttttaataga gtaagagctg ttcgactcca tgtttcattc attacatcaa
     4441 ctgtaaagga ttgatgattg aaatgagtaa ctgtaagaca gataccattt actgctatac
     4501 tatcgcctaa atggatatcc gttaatattt ttgaggcttt aattgatagt ttacaattac
     4561 gagagtcttt ctgtattctt tcaacttttc cgatttcttc aattattcct gtgaacatgg
     4621 ataaatcact tcactttcta tgagatagtc atttcctatt tgagaaaaag cataaggttt
     4681 caatctaata gcgtcatttg gcaaagaaat gccttcacct ccgacaggaa acttggcact
     4741 gcctccaaaa atttttggtg caatatatat tttcagctca tcaacaattt gttgttccaa
     4801 agcactccaa ttcattagac tgcccccttc tagaactagg ctatcaatct gcatgtttcc
     4861 tagatgttgc attaaactcg ataagtctat atgattgcct tttttcttta tggaaagtat
     4921 ttcacagcca tgattttgat atagcttcat tttatttttg tcttcagagg aagtggcaat
     4981 gtaagtttta atatcatttg ctgtttttac gattttagag gtaagaggag ttcgtaaatg
     5041 tgtatcgcat atgatacgga taggattttt cccttcctcc aatctacatg tcagcaaagg
     5101 atcgtcttga ataacagtat tgactcccac cataattgca ctaacatggt gtcgtaactg
     5161 atgcacatgc tttcttgctt cttcttcagt aatccatttg gattgatttg ttttagtggc
     5221 tatttttcca tccattgaca ttgcatattt cataaaaaca tagggtacat gctgggtaat
     5281 atactttcta aaacttttta ttaagttaag acactcattt tctaaaattc caacagtaac
     5341 ttgaagatta ttttcctcaa gtatctttac tccttttcca gatacaatag gattacagtc
     5401 taggcttcca atgactactc ttgtaatacc actatcgatt atagcatcta tacagggagg
     5461 tgttttcccg aagtgacaac agggttcaag tgttacataa agcgtcgctc cgacagggga
     5521 ttctctacag tttttaagag catttctctc agcatgtggg ccaccaaaaa actcatgata
     5581 accttgtccg atgatgtgat tatcttttac aataactgcg ccgaccatag gattgggatt
     5641 gacgtaacca gccccttttt gtgccagttt tattgctaat ttcatatatt ttgaatcgct
     5701 catctcacta cctccaaaaa aaatatacct tgaatagggg actactcaag gcatacaaaa
     5761 gaaaacttat gcgattaaca aaaatactct gaaatgacaa gtaatcattt cagagcacgc
     5821 aaaaagcaca aatatacttt tatcttcttt catccagact atactgtcgg ctttggaatt
     5881 tcaccaaatc atgcctttcg gctcgtgggc tataccaccg gtagggaatt acaccctgcc
     5941 ctgaagatag ttattcaatt acagatgatt atagtactta attttgaata tgtcaacaga
     6001 taaataccga ttgtttttga tatactgtat ttgtgataat cgattctcgc tcctcggata
     6061 aagaaaatat gatatactag ataaacgaaa taagagagaa ggaatactat gtacgcatat
     6121 ttaaaaggaa tcattaccaa aattactgcc aaatacattg ttcttgaaac caatggtatt
     6181 ggttatatcc tgcatgtggc caatccttat gcctattcag gtcaggttaa tcaggaggct
     6241 cagatttatg tgcatcaggt tgtgcgtgag gacgcccatt tgctttatgg atttcgctca
     6301 gaggatgaga aaaagctctt tcttagtctg atttcggtct ctgggattgg tcctgtatca
     6361 gctcttgcta ttatcgctgc tgatgacaat gctggcttgg ttcaagccat tgaaaccaag
     6421 aacatcacct acttgaccaa gttccctaaa attggcaaga aaacagccca gcagatggtg
     6481 ctggacttgg aaggcaaggt agtagttgca ggagatggcc ttcctgccaa ggtcgcagtg
     6541 caagcaagtg ctgaaaacca agaattggaa gaagctatgg aagccatgtt ggctctgggc
     6601 tacaaggcaa cagagctcaa gaaaatcaag aaattctttg aaggaacgac agatacagct
     6661 gagaactata tcaagtcggc ccttaaaatg ttggtcaaat aggagcagag aatgacaaaa
     6721 cgttgttcgt gggtcaagat gaccaaccca ctctacatcg cctatcatga tgaggagtgg
     6781 ggccagcccc tccatgatga ccaagcgttg tttgagttat tgtgtatgga aacctatcag
     6841 gcaggcctgt cttgggaaac ggtactcaac aaacgccaag ctttccgaga agcctttcat
     6901 agctatcaaa ttcactcagt cgcagagatg actgacactg aattggaagc catgctggag
     6961 aatccagcta tcattcgaaa tagagccaag atttttgcta cacgcgctaa cgcccaagct
     7021 tttctacagt tacaggcaga gtacggctct tttgatgcct atctttggtc ttttgttgag
     7081 gggaaaactg tcgttaacga tgttcctgat tatcgccaag ccccagctaa aacaccctta
     7141 tctgagaaat tagccaaaga tctcaaaaaa cgagacttca agttcacagg cccagtcgcc
     7201 gtattgtctt ttctacaggc tgcagggcta gttgatgacc acgagaatga ttgtgagtgg
     7261 aaaggtctta aatgatgtct aacaaaaata agggaattct gatttttgcg attctctata
     7321 cagtcctctt tgtgtttgat ggcgttaaat tgctggcttc tttaatgcca tctgccattg
     7381 caaattatct tgtttatgta gttttagctc tatatggctc cttcttattc aaggatagat
     7441 tgatccaaca atggaaggag attagaaaga ctaaaagaaa attcttcttt ggcgtcttaa
     7501 caggatggct ctttctcatt ctgatgactg ttgtctttga atttgtatca gagatgttga
     7561 agcagtttgt ggggctagtc ggacaaggtc taaatcagtc taatattcaa agcacttttc
     7621 aagagcaacc actactgata gctgtttttg cttgtgtcat tggacctctg gtagaagaat
     7681 tatttttccg tcaggtctta ttgcattact tgcaggaacg gttgccaggt ttactaagca
     7741 ttattctggt aggacttgtt tttgctctga ctcatatgca cagtttggct ctatcagagt
     7801 ggattggtgc agttggttac ttaggtggag gccttgcctt ttctattatt tatgtgaaag
     7861 aaaaagagaa tatctactat cccctacttg ttcacatgtt aagcaacagc ctctccttaa
     7921 tcattttagc tatca
//
LOCUS       DAVBCG010000088         7854 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBCG010000088 DAVBCG010000000
VERSION     DAVBCG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.088"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            287..2215
                     /gene="glgB"
                     /locus_tag="VQ495_001559"
     CDS             287..2215
                     /gene="glgB"
                     /locus_tag="VQ495_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2531497.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
     gene            2205..3347
                     /locus_tag="VQ495_001560"
     CDS             2205..3347
                     /locus_tag="VQ495_001560"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2531498.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3337..4476
                     /gene="glgD"
                     /locus_tag="VQ495_001561"
     CDS             3337..4476
                     /gene="glgD"
                     /locus_tag="VQ495_001561"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2531499.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            4473..5906
                     /gene="glgA"
                     /locus_tag="VQ495_001562"
     CDS             4473..5906
                     /gene="glgA"
                     /locus_tag="VQ495_001562"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2531500.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5965..6327
                     /locus_tag="VQ495_001563"
     CDS             5965..6327
                     /locus_tag="VQ495_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2531501.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            6281..6610
                     /locus_tag="VQ495_001564"
     CDS             6281..6610
                     /locus_tag="VQ495_001564"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2531502.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            6619..7734
                     /locus_tag="VQ495_001565"
     CDS             6619..7734
                     /locus_tag="VQ495_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2531503.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(7731..>7854)
                     /locus_tag="VQ495_001566"
     CDS             complement(7731..>7854)
                     /locus_tag="VQ495_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1694 domain-containing protein"
                     /protein_id="HET2531504.1"
                     /translation="IHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
BASE COUNT         2353 a         1352 c         1762 g         2387 t
ORIGIN      
        1 atagtacatt gaagttaaaa tagtacactt tgatttctaa gacattgtta gaaattagat
       61 taaattcctt aatcgatttg cgcatgtttt atttcatttc actatagtag aaaaagaaag
      121 gtgttttcgc aattttttga aaaaatattg atttacaatt gaacgagtaa gtgaacttgt
      181 tgtagatttt caaaataatt aaagaagtat agtaatttac ttgaatcttc tctaaaaagg
      241 tagtataata aaaacataga aaacgcttac aggagaaagg gagtatatgg ataatagaga
      301 agcattaaaa acctttatga cgggtgaaaa tttttatctc caacattatc taggagcaca
      361 tagggaagaa ctaaatggag agtatggcta taccttccgt gtttgggcac ctaatgctca
      421 ggctgttcac ttggttggtg attttaccaa ttggattgaa aatcagattc caatggtaag
      481 aaatgacttt ggggtctggg aagtctttac caatatggct caagaagggc atatttacaa
      541 atatcatgtc acacgtcaaa atggtcatca actgatgaag attgaccctt ttgctgtcag
      601 gtatgaggct cgtccaggaa caggggcaat cgtaacagag cttcctgata agaaatggag
      661 ggatggactt tggctggcac gaagaaaacg ttggggcttt gctgaacgtc ctgtcaatat
      721 ttacgaggtt catgctggct cttggaagag aaatcctgat ggcagtcctt atagttttgc
      781 ccaattgaaa gatgaactca ttccttacct agttgagatg aactatactc atattgagtt
      841 tatgcccttg atgtcccacc cactaggttt gagttggggg taccagctta tgggttactt
      901 cgctttagag catgcctatg gtagaccaga ggagtttcaa gattttgtcg aggagtgtca
      961 tacccataat attggggtta ttgtggactg ggtaccaggt cactttacca tcaatgatga
     1021 tgccttagcc tattatgatg ggacaccgac ttttgaatac caagaccata ataaggctca
     1081 taaccatggt tggggtgccc ttaattttga ccttggaaaa aatgaagtcc agtccttctt
     1141 aatttcttgc attaagcatt ggattgatat ctatcatttg gatggtattc gtgtggatgc
     1201 tgttagcaac atgctctatt tggactatga tgatgctcca tggacaccta ataaagatgg
     1261 cggaaatctc aactatgaag gttattattt ccttcaacgc ttgaatgaag ttattaagtt
     1321 agaatatcca gatgtgatga tgattgcaga agaaagttcg tctgcaacca agattacggg
     1381 aatgaaagag attggtggtc taggatttga ctacaaatgg aacatgggct ggatgaatga
     1441 tatcctccgt ttctacgaag aagatccgat ttatcgtaaa tatgacttta acctggtgac
     1501 tttcagcttt atgtatgttt tcaaggagaa ttatctcttg ccattctcgc acgatgaagt
     1561 ggttcatggc aagaagagta tgatgcataa gatgtgggga gatcgttaca atcaattcgc
     1621 aggcttgcgc aatctctata cgtaccaaat ttgtcaccct ggtaagaaat tgctcttcat
     1681 gggtagcgaa tacggtcaat tcctagaatg gaaatctgaa gaacagttgg aatggtctaa
     1741 cctagaagac ccaatgaatg ctaagatgaa gtatttcact tctcagctaa accagtttta
     1801 caaagatcat cgctgtctgt gggaaatcga taccagctat gatggtattg aagtcattga
     1861 tgcggataat cgagaccaga gtgttctttc ctttattcgt aagggtaaaa agggagaaat
     1921 gttagtctgt atctttaaca tggtacctgt tgagcggaaa gattttacaa tcggactacc
     1981 cgttgcagga atttacgaag aagtatggaa tactgagttg gaagagtggg gaggcgtttg
     2041 gaaagaacat aatcaaactg ttcaaacgca agaaggacta tggaaagatt atgagcagac
     2101 cttaaccttt accctaccgg ctatgggagc aagtgtatgg aaaatcaaac gtcgcttgaa
     2161 atctactaaa accgtcacaa ataaaaacca aaaaggagta gaaaatgaaa aatgaaatgt
     2221 tagctttgat tcttgctggt gggcaaggaa ctcgtctcgg taaactcact caaagcatcg
     2281 caaaaccagc tgtgcaattt ggtgggcgct accgtatcat tgactttgcc ctatcaaact
     2341 gtgccaactc agggattcat aatgttgggg tcgttacaca gtatcaacca cttgctctca
     2401 acaaccatat tgggaatggt tcaagctggg gactagacgg tattaattca ggtgtctcta
     2461 ttcttcaacc ttattctgca agtgaaggaa atcgttggtt tgaggggact agtcacgcta
     2521 tttaccaaaa tatcgactat atcgacagtg tcaatcctga gtatgtcttg attttgtctg
     2581 gggatcacat ctacaaaatg gactatgatg atatgctcca gtctcataag gataataatg
     2641 ccagcttgac agtagcagtt ttagacgtcc ctcttaaaga agcaagccgt tttggtatca
     2701 tgaacacaga tgctaacaat cgtattgttg aatttgaaga aaaaccagct caacctaaat
     2761 ctacaaaagc ttctatggga atctacattt ttgattggca acgccttcgt aatatgttag
     2821 tcgctgctga aaagagcaag gttggcatgt cagactttgg taaaaatgtc attccaaatt
     2881 accttgagtc aggtgaaagt gtttatgctt acgaatttag tggttattgg aaagatgttg
     2941 gtactattga gtcactttgg gaagcgaaca tggagtatat ttctccagaa aatgccttgg
     3001 atagtcgtaa ccgtcaatgg aagatttact caagaaactt gatttcacca ccaaacttcc
     3061 tcggggcaaa tgctcatgtg gaagactcat tagttgtaga cggatgtttc gttgatggaa
     3121 ctgttaaaca ttctatcctt tcaacaggcg cgcaagttcg cgaaggagcg gaagtccttg
     3181 attcagttat catgagtgga gctatcattg gtcaaggagc taagattaaa cgtgccatta
     3241 ttggtgaagg tgcgattatt tctgacggtg tcgaaattga tggaacagat gaagtacaag
     3301 ttgtaggata taatgaagta gtgggggtag caacagatga agattgataa atattctgcc
     3361 attttaggaa atacagttgg ttttcacaat atgtcgacat tgacggacca ccgtccagta
     3421 gcaagtttgc catttggtgg gaaatatcgt ttgattgact tcccactttc aagccttgct
     3481 aatgcaggtg ttcgtagtgt ctttggtatt ttccagcagg ataatatcag ctcagtattt
     3541 gaccatattc gttcaggacg cgagtggggc ttatcaaccc ttcttagcca ttactatcta
     3601 ggaatttaca atacccgtgt agaaagtagt acagttggaa aagaatacta ccaacagctt
     3661 cttacttatt tgaaacgttc tggctcaaac caaacggttg ccctcaactg cgatgttctt
     3721 attaacattg atttgaacca agttttccac ctacatagta caacaaaaga gcctatcact
     3781 gtagtttata aaaaactagc taagaaagat atttcagaag taaatgcaat cttggatgta
     3841 gatgaaacag accatgttct ttctcataaa ctctttgata gcaagtcaac agctgaaacg
     3901 ttcaatatgt ctacagatat ctttgtcgtt gatacacctt ggttgattga acacttggaa
     3961 gaagaagcta aaaaagaaca tccagagaaa ttgcgctatg ttttacggga tttggctgta
     4021 aaagagggag ctttcgccta cgagtacacg ggctatctag ccaatattca ctctgttaaa
     4081 tcttattatc aagcgaatat cgatatgctt gaatcacaaa aattctattc tcttttctca
     4141 ccaaaccaaa agatttatac aaaggtcaaa aacgaagagc caacttacta tgctaataca
     4201 tctaaggtaa gaacttctca gtttgcctct ggtagtatta ttgaaggtca agtagctaat
     4261 tctgttctat cacgtaatat ccatgtccat aaggatagct tggttaaaga tagcctgctc
     4321 ttccctcgtg ttgttattgg agaaggggct caggtcgaat atgctatctt ggacaaaggg
     4381 gttgaagttg agcctggtgt tgtgattcga ggaactgcag aacatccagt tgtggttaag
     4441 aaaggtgcta aagtaacaga ggatattcat tcatgaaaat tttatttgta gcagcagagg
     4501 gtgcaccctt ttcaaaaaca ggtggtttgg gagacgtcat tggcgctctt ccaaaatcac
     4561 tggtaaaagc tgggcacgaa gttgcagtga ttttacccta ctatgatatg gtagaggcta
     4621 aatttggaaa tcagattgaa gatgtgcttc attttgaggt gagcgttggt tggcgcagac
     4681 agtattgtgg aattaagaaa acagtattaa atggtgtaac cttctacttt attgacaatc
     4741 aatattattt cttccgtggt catgtttacg gtgattttga tgacggagaa cgctttgcct
     4801 ttttccaact ggctgccatt gaggctatgg aaaggattga ctttattcct gatcttctcc
     4861 atgttcatga ctaccataca gctatgattc ctttcttgtt gaaggaaaaa taccgttgga
     4921 ttcaagccta tgaggacatt gaaacagttt taaccattca taatttagaa ttccaaggac
     4981 aattttcaga aggaatgttg ggtgatttgt ttggagttgg ctttgaacgt tacgctgatg
     5041 gcacccttcg atggaacaac tgtctgaact ggatgaaggc aggtattctc tatgcgaacc
     5101 gtgtttcaac cgtttcacct agctatgctc atgaaattat gactagtcag tttggatgta
     5161 atttggatca gattcttaaa atggagtctg gtaaagtatc tggtatcgtg aatgggattg
     5221 atgctgatct ttataatcct cagacggatg ctcttttaga ctatcatttc aatcaggaag
     5281 atttgtctgg gaaagccaaa aataaggcaa aattgcaaga aagagttggc ttgcctgtta
     5341 gagcagacgt tccactggtg ggaattgttt ctcgtttgac acgtcaaaaa ggttttgatg
     5401 tggtggtcga aagtcttcac catatcttgc aagaagatgt tcagattgtt cttttgggaa
     5461 ctggcgatcc agcctttgaa ggagctttct catggtttgc tcagatttac ccagacaagc
     5521 tatcaacaaa tatcactttt gatgtcaaac ttgctcagga aatctacgct gcttgtgacc
     5581 tcttcctcat gccaagtcgt tttgaaccgt gtggcttgtc tcaaatgatg gctatgcgtt
     5641 atggaacctt gccattggtc catgaagttg gaggcttgcg agatacagtt cgcgctttca
     5701 atccaatcga aggaagcggt actggcttta gctttgacaa tctatctcct tattggttaa
     5761 attggacttt ccaaacagca ttggacttgt atagaaacca tccagacatt tggagaaacc
     5821 tacaaaaaca agctatggag agtgacttct catgggatac agcctgcaag tcataccttg
     5881 acttgtacca tagtttagtt aattaataga taaaatcgta tgatgacttc atacgatttt
     5941 taggctgttt agagaggaga actgatgtct caagtaaaag gcttgtgtgt tttggatgta
     6001 gatggaacct taatcctaga agaagtgatt gatcttttag ggagagaggc aggtcatgag
     6061 gcagaaattt cgcagattac aagtcgagca atgcgaggag agttggtctt tgaaagcagt
     6121 ttaagaaaaa gagtgtcctt gttggaaggt cttcctattt tggtctttga taacgtcttc
     6181 aactcaattc atctatcgct aaatgtccca gagttcatct ctattctcca aaagaatggt
     6241 atcctagttg gtctggtgtc tggtggattt acaccaatag ttggagagat tagcaaaatc
     6301 ccttggtatt gcctatttca ctgctaacca gcttgaagtc aaagaaggtc ttttaacagg
     6361 aaaattagtt ggacaaatta taagtcccca ggtcaaaaaa gaaactctgg aaaaatggag
     6421 aaagaaacta aaactttcta aagaaagaac ggtggcaatc ggtgatgggg gcaataatct
     6481 attaatgttg aagtcggcgg agttaggaat cgccttttgt tccaaagaaa tgctcaaaaa
     6541 agaaatacca catcatgttg acaagaggga ttttttagaa gttcttcctt tgattgactg
     6601 tttagaatga gggaaaatat gaagattgta attgcaccgg attcgtttaa ggaaagcttg
     6661 acagctcaac aggtagctga agcaataaaa agaggcttcc aacaatcgat agcagatgta
     6721 gaatgtctcc tctgccctgt tggtgatggg ggagaaggta ctgtagatgc tatccgacat
     6781 tctcttgacc taaaagaaaa atggatccaa gtgacagacc cttttggaca aaaagaagcc
     6841 atgcgctatt ttcaaaaagg ggaactggca ctatttgaag tagctgactt ggttggtctt
     6901 ggaaaaattc cgctagagaa acgaaatcca cttcaaatcc aaacttgtgg tattggagag
     6961 ttgattctcc atctcatttc taaagggatt aaagatattt atatcgatgt tggtggcacg
     7021 gccagtaatg atggaggact ggggattgct gctggtttag gttatcaatt ttatgatagg
     7081 gatggaaatg tcttgcctgc ttccggtcaa tccttattaa acttagcttc tgtttcaaca
     7141 gaaaattgct ataaaattcc tgaaggtgtt caaattcata ttttagcaga tgtcgtaagt
     7201 cccttatgtg gtcatcaagg tgcgacttat acttttggca atcaaaaagg tcgacatccg
     7261 actatgtttg cagtcgtaga tcaggcgatc caagattttt atgaaaaatt ctcacctgca
     7321 acattagaaa ttaaaggagc aggagcaggt ggaggccttg ctggtggttt gtgtgctttt
     7381 gctcaggcaa gtatcgtgtc tggaattgat acctgcttgg acttaatcaa ctttgataag
     7441 aaagtttcag atgctgactt ggttgttgtt ggagaaggaa gacttgatag tcaaagcttt
     7501 gctgggaaag cgcctatagg tgttgcaaaa agaacccctg ttggagttcc tgttattgct
     7561 atttgcggta gtcttgctga agatttacct cccctaccat ttgaaaatat ccaagctgtc
     7621 ttttctattt tggagaaaag tgaaccttta gaagatagtt tgaaaaatgc cagtctctat
     7681 ctggagcgca cggctgccaa tattgggcac ttattaaata tgtgcaagat ttagctaaac
     7741 catttcttcc ataaggatgt tttggctggt tcttcctttt taacttccca aagttttgga
     7801 aaagcaagtc gaaggtcttt ttcttctact tgaactggtg cattgctatg aata
//
LOCUS       DAVBCG010000089         7757 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBCG010000089 DAVBCG010000000
VERSION     DAVBCG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.089"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            570..1022
                     /locus_tag="VQ495_001567"
     CDS             570..1022
                     /locus_tag="VQ495_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2531505.1"
                     /translation="MIITKISHLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1041..2378
                     /locus_tag="VQ495_001568"
     CDS             1041..2378
                     /locus_tag="VQ495_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2531506.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2457..3341
                     /locus_tag="VQ495_001569"
     CDS             2457..3341
                     /locus_tag="VQ495_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2531507.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3341..4174
                     /locus_tag="VQ495_001570"
     CDS             3341..4174
                     /locus_tag="VQ495_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2531508.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4192..6285
                     /gene="nanB"
                     /locus_tag="VQ495_001571"
     CDS             4192..6285
                     /gene="nanB"
                     /locus_tag="VQ495_001571"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2531509.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6297..7400
                     /locus_tag="VQ495_001572"
     CDS             6297..7400
                     /locus_tag="VQ495_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2531510.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7574..>7757
                     /locus_tag="VQ495_001573"
     CDS             7574..>7757
                     /locus_tag="VQ495_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2531511.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIK"
BASE COUNT         2583 a         1193 c         1590 g         2391 t
ORIGIN      
        1 tgaaaacggt aacaaaacta atttagagaa tgaaatatag agtatttctc tcttaaaagt
       61 ttttggtgaa acgagatgta gaaaggagat ttagccaaag agtctattag tgctagaata
      121 atagattaga attattttag aaaaacgaag tgagcagctt ataaattcaa gtccccaaat
      181 agattcatac tagtatcttt tgcaaaaaat aaagggcgac ttccttcatg aatatcaatt
      241 tcatctataa ggaaggtagc taattgaact aacttattta ttctgtttgt cgctagaaaa
      301 atcagacctc cttgtgaaga ttgaggagat actgaatgaa aatcaaagaa gaaactagca
      361 agctagtagc agattgccca aaacaccgcc ttgaggttgt agataagact gacctatata
      421 atccaaggtg aagcgactgt ggtttgaaga gattttcaaa gagtataggc tagagagtag
      481 tgtttttatg tccttctagt agaaaatgct agacagaaga atggggaact tggataggaa
      541 aaatagattg agaaaggagg ttagaagaga tgattattac aaaaattagc catttaggaa
      601 cttatgtggg agtaaatcca cattttgcaa cattaataga ttttctagaa aaaacaggac
      661 tagaaaattt aacagaaggt tcgattgcta tcgatggtaa tcgattgttt gggaattgct
      721 ttacttatct agcagatggt caagcagggg ctttctttga aacacaccaa aaatatttgg
      781 atattcattt agttttggaa aacgaagaag ccatggctgt tacatcgccg gaaaatgtaa
      841 gcgttaccca agaatatgat gaagagaaag atattgaatt atacacaggg aaagtggaac
      901 agttggttca tttgagagct ggtgaatgcc tcatcacttt tccagaagat ttacatcaac
      961 ccaaggttcg tataaatgat gaacctgtga aaaaagttgt ctttaaagtt gcgatttctt
     1021 aatgtagaaa gagaagaacg atgaaaaaaa tgagaaagtt tttatgtcta gctggaattg
     1081 cgctagcggc tgttgccttg gtagcttgtt caggaaaaaa agaagctaca actagtactg
     1141 aaccaccaac agaattatct ggtgagatta caatgtggca ctcctttact caaggacccc
     1201 gtttagaaag tattcaaaaa tcagcagatg ctttcatgca aaagcatcca aaaacgaaaa
     1261 tcaagattga aacattttct tggaatgact tctatactaa atggactaca ggtttagcaa
     1321 atggaaatgt gccagatatc agtacagctc ttcctaacca agtaatggaa atggtcaact
     1381 cagatgcttt ggttccgcta aatgattcta tcaagcgtat tggacaagat aaatttaacg
     1441 aaactgcctt aaatgaagca aaaatcggag atgattacta ctctgttcct ctttattcac
     1501 atgcacaagt catgtgggtt agaacagatt tgttaaaaga acataatatt gaggttccta
     1561 aaacttggga tcaactctat gaagcttcta aaaaattgaa agaagctgga gtttatggct
     1621 tgtctgttcc gtttggaaca aatgacttaa tggcaacacg tttcttgaac ttctacgtac
     1681 gtagtggtgg aggaagcctc ttaacaaaag atcttaaagc agacttgaca agccaacttg
     1741 ctcaagatgg tattaaatac tgggttaaat tgtataaaga aatctcacct caagattctt
     1801 tgaattttaa tgtccttcaa caagctacct tgttctatca aggaaaaaca gcatttgact
     1861 ttaactctgg cttccatatc ggaggaatta atgccaacag tcctcaattg attgattcga
     1921 ttgatgctta tcctattcca aaaatcaaag agtctgataa agaccaagga attgaaacct
     1981 caaacattcc aatggttgtt tggaaaaatt caaaacatcc agaagttgct aaagcattct
     2041 tagaagcact ttataatgaa gaagactacg ttaaattcct tgattcaact ccagtaggta
     2101 tgttgccaac tattaagggg attagcgatt ctgcagccta taaagaaaat gaaactcgta
     2161 agaagtttaa acatgctgaa gaagtaatta ctgaagctgt taaaaaaggt actgctattg
     2221 gttatgaaaa tggaccaagt gtacaagctg gtatgttgac taaccaacac attattgaac
     2281 aaatgttcca agatatcatt acaaatggaa cagatcctat gaaagcagca aaagaagcag
     2341 aaaaacaatt aaacgattta tttgaggctg ttcagtagat gtaaaagact agaaaatagg
     2401 tgggctagtg atctgaaaag ctctagccca attttgtaaa agaagggaga aggagaatgg
     2461 ttaaagaacg taatttaact cgctggatat ttgttttgcc agctatgatt atcgtaggat
     2521 tactctttgt ttatccgttt ttctcgagta ttttttatag ctttaccaat aagcatttga
     2581 ttatgcctaa ttataaattt gttggtttgg ctaactataa agctgtgcta tcagatccca
     2641 acttctttaa tgcgttcttt aattcaatta agtggaccgt tttctcatta gttggtcaag
     2701 ttttagtagg gtttgtattg gctttagctc ttcacagagt acgccacttc aagaaattat
     2761 ataggacctt attgattgtt ccttgggcat ttcctaccat cgttattgcc ttctcttggc
     2821 agtggattct aaacggggtt tatggctact tacctaatct aatcgtaaaa ttaggtttaa
     2881 tggaacatac acctgcattt ttgacagata gtacatgggc attcctatgt ttggtgttta
     2941 tcaacatttg gtttggagca ccaatgatta tggttaatgt gctttcagct ttgcaaacag
     3001 taccagaaga acaatttgag gctgctaaga tagatggtgc ttcaagttgg caggtgttca
     3061 agtttatcgt ctttccacat attaaagtgg ttgtaggact tctagttgtt ttgagaactg
     3121 tatggatctt taataacttt gacattatct acctcattac tggtggtgga ccagccaatg
     3181 ctacaacgac gcttccaatt tttgcttaca acctgggctg gggaactaaa ttgttgggtc
     3241 gtgcttcagc agttacagta ctgctcttta tcttcttggt ggcgatttgc tttatctact
     3301 ttgctatcat cagtaagtgg gaaaaggagg gtagaaaata atgaagaaga aatccagtat
     3361 ttatttagat attctctcac atgtactttt agttggtgcg accatcgttg cagttttccc
     3421 attggtatgg attatcatat cttctgtcaa agggaaaggg gaattaactc agtatccaac
     3481 acgattttgg cctgaacagt ttacattaga ttatttcact catgttatca acgatttgca
     3541 cttcattgat aacattcgaa acagtttaat cattgccttg gctacaaccc ttattgcgat
     3601 tattatttct gctatggcag cctatggtat tgttcgattc tttcctaaat tgggagcaat
     3661 catgtcgaga ctactcgtca ttacctacat tttcccacca attttgttag caattcccta
     3721 ttcaattgcc attgctaaag ttgggttaac aaatagttta tttggcttga tgatggttta
     3781 tctatctttt agtgttccat atgcagtttg gctcttagtt ggatttttcc aaacagttcc
     3841 aattggaatt gaagaagcgg ctagaattga tggtgcaaat aaatttgtta cgttttataa
     3901 agttgtgcta ccgattgtag caccaggtat tgtagcaaca gctatttata catttatcaa
     3961 tgcttggaat gaattcctgt atgccttgat tttgattaac aatacaggaa agatgacagt
     4021 agcagtagcc cttcgttcac ttaatggttc agaaatacta gactggggag atatgatggc
     4081 agcgtctgtt attgtagttc ttccatcaat tattttcttc tctatcatcc aaaataagat
     4141 tgcaagtgga ttatcagaag gatctgtgaa gtagacgaaa gaaggaaaaa aatgaataaa
     4201 agaggtcttt attcaaaact aggaatttcc gttgtaggca ttagtctttt aatgggagtc
     4261 cccactttga ttcatgcgaa tgaattaaac tatggtcaac tgtccatatc tcctattttt
     4321 caaggaggtt catatcaact gaacaataag agtatagata tcagctcttt gttattagat
     4381 aaattgtctg gagagagtca gacagtagta atgaaattta aagcagataa accaaactct
     4441 cttcaagctt tgtttggcct atctaatagt aaagcaggct ttaaaaataa ttacttttca
     4501 attttcatga gagattctgg tgagataggt gtagaaataa gagacgccca aaagggaata
     4561 aattatttat tttccagacc agcttcatta tggggaaaac ataaaggaca ggcagttgaa
     4621 aatacactag tatttgtatc tgattctaaa gataaaacat acacaatgta tgttaatgga
     4681 atagaagtgt tctctgaaac agttgataca tttttgccaa tttcaaatat aaatggtata
     4741 gataaggcaa cactaggagc tgttaatcgt gaaggtaagg aacattacct cgcaaaagga
     4801 agtattgatg aaatcagtct atttaacaaa gcaattagtg atcaggaagt ttcaactatt
     4861 cccttgtcaa atccatttca gttaattttc caatcaggag attctactca agctaactat
     4921 tttagaatac cgacactata tacattaagt agtggaagag ttctatcaag tattgatgca
     4981 cgttatggtg ggactcatga ttctaaaagt aagattaata ttgccacttc ttatagtgat
     5041 gataatggga aaacgtggag tgagccaatt tttgctatga agtttaatga ctatgaggag
     5101 cagttagttt actggccacg agataataaa ttaaagaata gtcaaattag tggaagtgct
     5161 tcattcatag attcatccat tgttgaagat aaaaaatctg ggaaaacgat attactagct
     5221 gatgttatgc ctgcgggtat tggaaataat aatgcaaata aagccgactc aggttttaaa
     5281 gaaataaatg gtcattatta tttaaaacta aagaagaatg gagataacga tttccgttat
     5341 acagttagag aaaatggtgt cgtttataat gaaacaacta ataaacctac aaattatact
     5401 ataaatgata agtatgaagt tttggaggga ggaaagtctt taacagtcga acaatattcg
     5461 gttgattttg atagtggctc tttaagagaa aggcataatg gaaaacaggt tcctatgaat
     5521 gttttctaca aagattcgtt atttaaagtg actcctacta attatatagc aatgacaact
     5581 agtcagaata gaggagagag ttgggaacaa tttaagttgt tgcctccgtt cttaggagaa
     5641 aaacataatg gaacttactt atgtcccgga caaggtttag cattaaaatc aagtaacaga
     5701 ttgatttttg caacatatac tagtggagaa ctaacctatc tcatttctga tgatagtggt
     5761 caaacatgga agaaatcctc agcttcaatt ccgtttaaaa atgcaacagc agaagcacaa
     5821 atggttgaac tgagagatgg tgtgattaga acattcttta gaaccactac aggtaagata
     5881 gcttatatga ctagtagaga ttctggagaa acatggtcga aagtttcgta tattgatgga
     5941 atccaacaaa cttcatatgg cacacaagta tctgcaatta aatactctca attaattgat
     6001 ggaaaagaag cagtcatttt gagtacacca aattctagaa gtggccgtaa gggaggtcaa
     6061 ttagttgtcg gtttagtcaa taaagaagat gatagtattg attggaaata ccactatgat
     6121 attgatttgc cttcgtatgg ttatgcctat tctgcgatta cagaattgcc aaatcatcac
     6181 ataggtgtac tgtttgaaaa atatgattcg tggtcgagaa atgaattgca tttaagcaat
     6241 gtagttcagt atatagattt ggaaattaat gatttaacaa aataaaggag aaaaacatgg
     6301 ttaaatacgg tgttgttgga gcagggtatt ttggagctga attggctcgc tacatgcaaa
     6361 agaatgatgg agcagagatt actcttctct atgatccaga taatgcagag gcgattgcag
     6421 aagaattggg ggcaaaagta gcaagttcct tagatgagtt ggtttctagc gatgaagtag
     6481 attgtgttat cgtcgcaact ccaaataatc ttcataagga accggttatt aaggctgcac
     6541 agcatggtaa aaatgttttc tgtgaaaaac caattgcgct ttcttatcaa gattgtcgcg
     6601 agatggtaga tgcgtgtaaa gaaaacaatg taacctttat ggcaggacat attatgaatt
     6661 tctttaatgg tgttcatcat gcaaaagaac tcattaatca aggagttatc ggagacgttc
     6721 tatattgtca tacagctcgt aatggttggg aagaacaaca accgtcagta tcatggaaaa
     6781 aaattcgtga aaaatcaggt ggtcacttgt atcaccacat ccatgaattg gattgcgttc
     6841 aattccttat ggggggcatg cctgaaactg taaccatgac aggtggaaat gtggcccatg
     6901 aaggtgaaca tttcggtgat gaagatgata tgatttttgt caatatggaa ttttctaata
     6961 agcgttttgc cttgttagaa tggggttcag cttatcgttg gggtgaacat tatgtcttaa
     7021 tccaaggaag caaaggtgcc atccgcttag acttattcaa ctgtaaagga actcttaagc
     7081 tagatgggca agaaagctat ttcttgattc acgaatcgca agaagaagat gatgatcgga
     7141 ctcgtatcta tcatagtaca gagatggatg gagcaattgc ttatggtaaa ccaggtaaac
     7201 gtactccatt atggctatca tctgtcattg ataaagaaat gcgctatctg catgagatta
     7261 tggaaggagc tccagtatca gaagaatttg caaaactttt gacaggtgaa gctgctctag
     7321 aagcaattgc tactgcagat gcttgtaccc agtctatgtt tgaagatcgc aaagtaaaat
     7381 tgtcagaaat tgtaaaataa attttggtat tctcctattt ataggtcgac ttgctcctct
     7441 gaaagtactt ttagaggagc tgtttgactt tgctagtttt tgaaactgaa atctattata
     7501 ctacaaacta ttgaaagcgt tttaatttta aggtataata atctcataga aataaagaaa
     7561 aggaggaaag aggatgccac agattagcaa agaagccttg attgagcaaa tcaaagatgg
     7621 aatcatcgtt tcttgtcagg ctcttcctca tgaaccgctt tatacagaag cgggaggggt
     7681 gattcccttg ctggtcaaag cggctgagca aggtggagca gtcggtatcc gagcaaacag
     7741 tgttcgcgat atcaagg
//
LOCUS       DAVBCG010000090         7684 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBCG010000090 DAVBCG010000000
VERSION     DAVBCG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.090"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(363..2054)
                     /locus_tag="VQ495_001574"
     CDS             complement(363..2054)
                     /locus_tag="VQ495_001574"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2531512.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2190..2636
                     /gene="argR"
                     /locus_tag="VQ495_001575"
     CDS             2190..2636
                     /gene="argR"
                     /locus_tag="VQ495_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2531513.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2687..5221
                     /gene="mutS"
                     /locus_tag="VQ495_001576"
     CDS             2687..5221
                     /gene="mutS"
                     /locus_tag="VQ495_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2531514.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5368..7062
                     /gene="patA"
                     /locus_tag="VQ495_001577"
     CDS             5368..7062
                     /gene="patA"
                     /locus_tag="VQ495_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HET2531515.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(7392..>7684)
                     /locus_tag="VQ495_001578"
     CDS             complement(7392..>7684)
                     /locus_tag="VQ495_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET2531516.1"
                     /translation="RRFISCNEIIKHYKRLIDKAEKCVNDLMAEFNSVITTVTGIGNR
                     LGAVILAEIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMVKRGSPHLR"
BASE COUNT         2107 a         1492 c         1798 g         2287 t
ORIGIN      
        1 attatcaatc atgaactagg attctagttt tgcaggaagt ctattatttg gttaggtgaa
       61 ttagtgaagc gtttaggcaa gtgtctctgg ttacgacgtc atggactcta aatcgattat
      121 atttaggggt catgactagt gaagcagtta gctagttcgc atataagcgg ctagcgtcta
      181 acaattagga actttagttc caataacttt aagattacga cgttttagga cataaatcga
      241 tcatatttat gtcctaaaac tagtgaagta tgtcctaaaa ctagtgaagc gcctagccaa
      301 agtccgaata ggatttggcg ttagttactt agattgcttt gcaatcaagt aactttggcg
      361 atttacatct tctctggcgc ttctactcca agcaagcgaa gggcttcttt gagaacgact
      421 gcggttgcgt agctgagggc tagacggctg tcgcgttctg ggctttcatc caagatacgt
      481 gtatgtgcat agtatttgtt aaaggattga gccaggctaa ttgcaaattt agcaatgata
      541 gaaggttcaa agttatctgc cgcacggttg ataatacgtg ggaagtcttg aatgagttta
      601 atgatttccc agctttcagt atcattcaag ctatagttgc cagctgtttc tggtttgaaa
      661 tcggctttgc gtaagataga ttggatacga gcgtaggcat attgaacgta aggtccagtt
      721 tcaccctcga aggataccat agcctctagg tcgaagtcgt atccatttgt acggtcggtt
      781 ttgaggtcat agaatttaat ggctccaacc ccaacagcat gtgctacttg gtctttgttt
      841 tctagttcag gatttttagc ctcgatttgg accttggcac ggctaacagc ctctgcaaca
      901 gtaggctcta gcaagatgac attcccttta cgagtagaga gtttcttccc ttcttttgta
      961 accaaaccaa aaggaacgtg agtaatgtcg tcactccagt cgtagcccat ctcttgcaag
     1021 acagctttga gctgtttaaa gtgggcagat tgttcttgac caacgacata gatagattta
     1081 gcaaattggt attcgttttt acggtagagg gctgcagcca agtcacgtgt gatatagaga
     1141 gttgcaccat cagacttctt gatgagggct ggatgttcaa ttccgtattt ctcaagattc
     1201 acaacttggg caccttctga ttcaagaagt agtccttttt cagaaagaat gtctacaact
     1261 gcatccatct tatcattgta gaaggcttct ccgttatagc tgtcaaattc aaccttcaat
     1321 tcattgtaaa ggcggttaaa ttccactaaa ctttcatcgc ggaaccattg ccaaagagcg
     1381 agagcttcct catctccatt ttcaagttta cggaaccatt cgcgcgcttc ttcatccaag
     1441 ctagggtcat tttcagcttc agcgttgatg cggacataga gtttaaggag ttcatcgatt
     1501 ggatgagctt ttacagcttc ttcgtcgccc cattttttgt aggcaacaat caacatccca
     1561 aattgtttac cccagtctcc caaatggttg accttgaccg tttgataacc gattttttgg
     1621 aaaatatgtg acaagctatc tccgataaca gttgaacgca ggtggccaat agaaaatggt
     1681 ttagcgatat tcggactaga catgtcgata acaacatttt cttgtttacc aatattttgg
     1741 tcagcatagt gttctttttc agtggtaaca gcttgcaata cttgagcaga aatggcagat
     1801 ttatcaagga aaaagttaac gtaaggtcct gttgcgacaa ctttttcaaa ggcttggctg
     1861 ttcatttttt cagccagttc agccgcaatc atttgtggtg ctttacgttc gactttggca
     1921 agagaaaaag cagggaaagc gatgtcaccc atttctgagt ttttaggggt ttccagtaac
     1981 tttaaaatag cctcttggtc caggctatca atgatgctag ccaattcgct agcaatcaat
     2041 tcttttgtat tcattaagag ctcctttttg gacttttcta ctattttatc acaattttaa
     2101 agaaagaaga aaaaattttt gaaatctcct gtttttttgg tataatatgg ttataaatat
     2161 agttataaat atgcacgcaa gaggatttta tgagaaaaag agatcgtcat cagttaataa
     2221 aaaaaatgat tactgaggag aaattaagta cacaaaaaga aattcaagat cggttggagg
     2281 cgcacaatgt ttgtgtgacg cagacaacct tgtctcgtga tttgcgcgaa atcggcttga
     2341 ccaaggtcaa gaaaaatgat atggtgtatt atgtactagt aaatgagaca gaaaagattg
     2401 atttggtgga atttttgtct catcatttag aaggtgttgc aagagcagag tttaccttgg
     2461 tgcttcatac caaattggga gaagcctctg ttttggcaaa tattgtagat gtaaataagg
     2521 atgaatggat tttaggaaca gttgctggtg ccaatacctt attggttatt tgtcgagatc
     2581 agcacgttgc caaactcatg gaagatcgtt tgctagattt gatgaaagat aagtaaggtc
     2641 ttgggagttg ctctcaagac ttatttttga aaaggagaga cagaaaatgg cgatagaaaa
     2701 gctatcaccc ggcatgcaac agtatgtgga tattaaaaag caatatccag atgctttttt
     2761 gctctttcgg atgggtgatt tttatgaatt attttatgag gatgcggtca atgctgcgca
     2821 gattctggaa atttccttaa cgagtcgcaa caagaatgcc gacaatccga tccctatggc
     2881 gggtgttccc tatcattctg cccaacagta tatcgatgtc ttgattgagc agggttataa
     2941 ggtggctatc gcagagcaga tggaagatcc taaacaagca gttggggttg ttaaacgaga
     3001 ggttgttcag gtcattacgc cagggacagt ggtcgatagc agtaagctgg acagtcagaa
     3061 taattttttg gttgccatag accgcgaagg caatcaattt ggcctagctt atatggattt
     3121 ggtgacgggt gacttttatg tgacaggtct tttggatttc acgctggttt gtggggaaat
     3181 ccgtaacctc aaggctcgag aagtggtgtt gggttatgac ttgtctgagg aagaagaaca
     3241 aatcctcagc tgccagatga atctggtact ctcttatgaa aaagaaagct ttgaagacct
     3301 tcatttattg gatttgcgat tggcaacggt ggagcaaacg gcatctagta agctgctcca
     3361 gtatgttcat cggactcaga tgagggaatt gaaccacctc aaacctgtta tccgctacga
     3421 aattaaggat ttcttgcaga tggattatgc gaccaaggct agtctggatt tggttgagaa
     3481 tgctcgctca ggtaagaaac aaggcagtct tttctggctt ttggatgaaa ccaaaacggc
     3541 tatggggatg cgtctcttgc gttcttggat tcatcgcccc ttgattgata aggaacgaat
     3601 cgtccaacgt caagaagtag tgcaggtctt tctcgaccat ttctttgagc gtagtgactt
     3661 gacagacagt ctcaagggtg tttatgacat tgagcgcttg gctagtcgtg tttcttttgg
     3721 caaaaccaat ccaaaggatc tcttgcagtt ggcgactacc ttgtctagtg tgccacggat
     3781 tcgtgcgatt ttagaaggga tggagcaacc tgctctagcc tatctcatcg cacaactgga
     3841 tgcaatccct gagttggaga gtttgattag cgcagcgatt gctcctgaag ctcctcatgt
     3901 gattacagat gggggaatta tccggactgg atttgatgag actttagaca agtatcgttg
     3961 cgttctcaga gaagggacta gctggattgc tgagattgag gctaaggagc gagaaaactc
     4021 tggtatcagc acgctcaaga ttgactacaa taaaaaggat ggctactatt ttcatgtgac
     4081 caattcgcaa ctgggaaatg tgccagccca ctttttccgc aaggcgacgc tgaaaaactc
     4141 agaacgcttt ggaaccgaag aattagcccg tatcgaggga gatatgcttg aggcgcgtga
     4201 gaagtcagcc aacctcgaat acgaaatatt tatgcgcatt cgtgaagagg tcggcaagta
     4261 catccagcgt ttacaagctt tagcccaagg aattgcgacg gttgatgtct tacagagtct
     4321 ggcggttgtg gctgaaaccc agcatttgat tcgacctgag tttggtgacg attcacaaat
     4381 tgatatccgg aaagggcgcc atgctgtcgt tgaaaaggtt atgggggctc agacctatat
     4441 tccaaatacg attcagatgg cagaagatac cagtattcaa ctgattacag ggccaaacat
     4501 gagtgggaag tctacctata tgcgtcagtt agccatgacg gcggttatgg cccagctggg
     4561 ttcctatgtt cctgctgaaa gcgcccattt accgattttt gatgcgattt ttacccgtat
     4621 cggagcagca gatgacttgg tttcgggtca gtcaaccttt atggtggaga tgatggaggc
     4681 caataatgcc atttcgcatg cgaccaagaa ctctctcatt ctctttgatg aattgggacg
     4741 tggaactgca acttatgacg ggatggctct tgctcagtcc atcatcgaat atatccatga
     4801 gcacatcgga gctaagaccc tctttgcgac ccactaccat gagttgacta gtctggagtc
     4861 tagtttacaa cacttggtca atgtccacgt ggcaactttg gagcaggatg ggcaggtcac
     4921 cttccttcac aagattgaac cgggaccagc tgataaatcc tacggtatcc atgttgccaa
     4981 gattgctggc ttgccagcag accttttagc aagggcggat aagattttga ctcagctaga
     5041 gaatcaagga acagagaatc ctcctcccat gagacaaact agtgctgtca ctgaacagat
     5101 ttcactcttt gatagggcag aagagcatcc tatcctagca gaattagcta aactggatgt
     5161 gtataatatg acacctatgc aggttatgaa tgtcttagta gagttaaaac agaaactata
     5221 aaaccaagac tcactagtta atctagctgt agcaaggaga cttctttgac aattctccac
     5281 ttttttgcta gaatgacatc acacaaacag aatgaaaagg agctgacgca ttgtcgctcc
     5341 cttttgtcta ttttttaagg agaaagtatg ctgattcaga aaataaaaac ctacaagtgg
     5401 caggccctgg cttcgctcct gatgacaggc ttgatggttg ctagttcact tctgcaaccg
     5461 cgttatctgc aggaagtctt aggcgccctc cttactggga aatatgaagc tatttatagt
     5521 atcggggctt ggttgattgg tgtggccgta gtcggtctag ttgctggtgg actcaatgtt
     5581 gtcctcgcag cctatattgc ccaaggagtt tcatccgacc ttcgggagga tgccttccgt
     5641 aaaattcaaa ccttttctta tgctgatatt gaacaattta atgcgggaaa tctagtcgtt
     5701 cgaatgacaa atgatatcaa ccagattcag aacgttgtca tgatgacctt ccagattctt
     5761 ttcagacttc ccctcttgtt catcggttcg tttatcctag cggttcaaac cttaccttct
     5821 ctgtggtggg tgattgttct catggtagtc ttgatttttg gtttgactgc tgtcatgatg
     5881 ggaatgatgg ggcctcgttt tgccaagttt caaacccttc ttgagcgcat caatgccatt
     5941 gccaaggaaa atttacgtgg cgttcgtgtg gtcaagtcct ttgtccaaga aaaagagcaa
     6001 tttgctaagt ttacagaggt ctcagacgag cttcttggtc aaaaccttta cattggttat
     6061 gccttttcag tagtggaacc ctttatgatg ttggttggtt acggggcggt cttcctctct
     6121 atttggctgg tcgcgggaat ggttcagtcg gatccgtctg ttgttggttc catcgcttct
     6181 tttgttaatt acctaagcca gattatcttt accattgtta tggttggatt tttgggaaat
     6241 tctgtcagcc gtgccatgat ttccatgcgt cgtattcgag aaattcttga cgcagagcca
     6301 gctatgacct tcaaggatgt cccagatgaa gagttggttg gaagccttag ctttgaaaat
     6361 gtgaccttta cctatccaat ggacaaggaa ccgatgctga aagatgtgag ctttactatt
     6421 gaacctggtc aaatggttgg tgtagttgga gcgactggtg caggaaagtc aaccttggct
     6481 caattgattc cacgtctctt tgatccacag gaaggggcta tcaaaatcgg aggtaaggat
     6541 attcgagaag tgagtgaagg aaccctgcgt aaaacagttt ccatcgttct ccaacgtgcc
     6601 attcttttta gtggaacgat tgcagataac ttgagacagg ggaagggaaa tgctactcta
     6661 tttgaaatgg agcgcgcagc caatattgcc caggctagtg aattcattca tcgtatggag
     6721 aaaacctttg aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg acaaaaacaa
     6781 aggatgtcga ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt tgatgattcg
     6841 acctcagcct tggatgccaa atcagagcgc ttggtgcaag aagctttgaa taaggacttg
     6901 aaggggacga caaccattat tattgctcaa aaaattagct cggttgtcca tgcagacaag
     6961 atcttggttc taaatcaagg acgattgatt ggtcaaggca cgcatgcaga cttggttgcc
     7021 aacaatgccg tttaccgtga aatctatgaa acacagaaat gaaagacaaa ctataagaaa
     7081 agtcaatagt tttatctaaa ctatttctta tttcaatttg atgatttggc gatgatttta
     7141 gagcacggca aaaagccctt gaaaaagtcc attttttcaa aggtaatcct gtgttgattt
     7201 cagaaattac atcacttttt gttcgtcaaa tggcagctct tttttaggat ataaaacagg
     7261 gttcggataa gtttttttgc aaggtggatg atggctacat tgtaatgttt tccttgttat
     7321 aacttagtct taagataggc cttaaaagca ggtgaaaagt gagggcatgc tttggcagct
     7381 tgtatgagta cctaccgcag atgaggggaa ccccgtttga ccattcttcc agctaaatca
     7441 atctgacctg actgataaat agaagaatcc agtccagcga aagcttgtaa ttgagcagga
     7501 ttatcaaagg catgaatatt tcgaatctcg gctaaaatga ccgcccctaa acgattccca
     7561 atcccagtaa ccgtcgtgat gaccgagttg aactcagcca tcaagtcatt gacacatttt
     7621 tccgccttgt caatgagcct cttgtaatgt ttgatgattt cattacacga gataaaacgt
     7681 ctat
//
LOCUS       DAVBCG010000091         7549 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBCG010000091 DAVBCG010000000
VERSION     DAVBCG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.091"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            172..1563
                     /locus_tag="VQ495_001579"
     CDS             172..1563
                     /locus_tag="VQ495_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2531517.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            1677..2519
                     /locus_tag="VQ495_001580"
     CDS             1677..2519
                     /locus_tag="VQ495_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2531518.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            2792..3553
                     /locus_tag="VQ495_001581"
     CDS             2792..3553
                     /locus_tag="VQ495_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2531519.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            3923..4840
                     /gene="glyQ"
                     /locus_tag="VQ495_001582"
     CDS             3923..4840
                     /gene="glyQ"
                     /locus_tag="VQ495_001582"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2531520.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            5100..7136
                     /locus_tag="VQ495_001583"
     CDS             5100..7136
                     /locus_tag="VQ495_001583"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2531521.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            7178..7435
                     /locus_tag="VQ495_001584"
     CDS             7178..7435
                     /locus_tag="VQ495_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2531522.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         2365 a         1325 c         1688 g         2171 t
ORIGIN      
        1 aggtctacta ttctagcttc aatctactag aaattccata gatagaaaac tacataatct
       61 ctacagatac ggatgttgga gttgatgtaa gatgctttgg cttgctagag gaattgtgga
      121 ttgccaaatt gtatcattga aattattgct caaatttgtt atgatataaa tatgaataaa
      181 agtagactag gacgtggcag acacgggaaa acgagacatg tattattggc tttgattggt
      241 attttagcaa tttctatttg cctattaggc ggatttattg cttttaagat ctaccagcaa
      301 aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga aagatgatca attgagtgag
      361 ggaaatcaga aggagcattt tcgtcagggg caagccgaag tgattgccta ttatcctctc
      421 caaggggaga aagtgatttc ctctgttagg gagctgataa atcaagatgt taaggacaag
      481 ctagaaagta aggacaatct tgttttctac tatacagagc aagaagagtc aggtttaaag
      541 ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt tagttgcttt taagattgaa
      601 gagactgaaa agaccagtct aggaaaggtt cacttaacag aagatgggca accttttaca
      661 cttgaccaac tgttttcaga tgctagtaag gctaaggaac agctgataaa agagttgacc
      721 tccttcatag aggataaaaa aatagatcaa gaccagagtg agcagattgt aaaaaacttc
      781 tctgaccaag acttgtctgc atggaatttt gattacaagg atagtcagat tatcctttat
      841 ccaagtcctg tggttgaaaa tttagaagag atagccttgc cagtatctgc tttctttgat
      901 gttatccaat cttcgtactt actcgaaaaa gatgcggcct tgtaccaatc ttactttgat
      961 aagaaacatc aaaaagttgt cgctctaacc tttgatgatg gtccaaatcc agcaacgacc
     1021 ccgcaggtat tagagaccct agctaaatat gatattaaag cgactttctt tgtgcttggg
     1081 aaaaatgttt ctgggaatga ggacttggtg aagaggataa aatctgaagg tcatgttgtt
     1141 ggaaaccata gttggagcca tccgattctc tcgcaactct ctcttgatga agctaaaaag
     1201 cagattactg atactgagga tgtgctaact aaagtgctgg gttctagttc taaactcatg
     1261 cgtccacctt atggtgctat tacagatgat attcgcaata gcttggattt gagctttatc
     1321 atgtgggatg tggatagtct ggactggaag agtaaaaatg aagcatctat tttgacagaa
     1381 attcagcatc aagtagctaa cggctctatc gttttgatgc atgatattca cagtccgaca
     1441 gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc aaggttatac ctttgtgacc
     1501 ataccagaga tgctcaatac tcgcctaaaa gctcatgagc tgtactatag tcgtgatgaa
     1561 taagcaagaa aaaataggtc tgttagatat ttgacagact tattttttac agaatatagt
     1621 actacttaaa aaatgtttta tgctataatt gatgaataaa atagaaggag aagcatatga
     1681 atacctatca attaaataat ggagtagaaa ttccagtatt gggatttgga acttttaagg
     1741 ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc cttgaaggct ggttatcgtc
     1801 atattgatac ggcggcgatt tatcagaatg aagaaagtgt tggtcaagca atcaaagata
     1861 gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct ttggaatagt cagcaaacct
     1921 atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa actgggcttg gattatttgg
     1981 atttgtattt gattcattgg ccgaacccaa aaccgctcag agaaaatgac gcatggaaaa
     2041 ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta tcaagaaggg aaaatccgtg
     2101 ctatcggcgt tagcaatttt cttccccatc atttggatgc cttgcttgaa actgcaacta
     2161 tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt gtatcaagaa caagtcgtag
     2221 cttactgtcg tgaaaaggga attttattgg aagcttgggg gccttttggt cagggagaac
     2281 tatttgatag caagcaagtg caagaaatcg cagcaaatca cggaaaatcg attgctcaga
     2341 tagccttggc ctggagcttg gcagaaggat ttttaccact tccaaaatct gtcacaacct
     2401 ctcgtattca agctaatctt gattgctttg gaattgaact gagtcatgag gagagagaaa
     2461 ccttaaaaac gattgctgtt caatcgggtg ctccacgagt tgatgatgtg gatttctaga
     2521 aaatcataaa aagaattgta cattattcta atttttgata taatagtcag caggaaagaa
     2581 agtcttatgg cgttcttcaa gcgagcttgg gatagtggga gccaagtagg gcaaaataat
     2641 gagctggcgc tttctgtagt attttcaaaa acaatgaagt aataaattag ggtggaaccg
     2701 cgtttctgac gcccctaggt taaatcaacc taggattgtc ggacgcggtt cttttgctta
     2761 ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac ctttatcttg taaaagacga
     2821 tagtcaacta gctacatttc gtgattttgt agtaagaaat actgaaaagt tgaaagatta
     2881 tcaatctttt ttaaagaatg aacttgcagt ctgtgattta ccgcaagccg ttatttggtc
     2941 agattttaat gctgctacac agattattag ggaaagcgct gttccagcct atacaaataa
     3001 tagacgaatg gttatggcgc ctgatttagc tgtttggaaa gagttgtatt tgtatcaatt
     3061 gatggactac gagtgttctc agcaaactca agcaatagaa agtcactatc attctttatc
     3121 tgaaaatttc ctcttacaga ttgtaggaca tgagttagct cattggtcgg agcatttttt
     3181 agatgatttt gatggttatg actcttatat ctggttcgaa gaggggatgg ttgaatatat
     3241 tagtcgcaag tatttcttga cagaagagga atttcaagcg gaaaaaattt gtaatcaatc
     3301 tctcgtagaa ctttttcaga agaagtatgg ttggcattca ttgaatgatt ttggttcttc
     3361 gacttatgat aagaactatg caagtatttt ttatgaatac tggcgcagct ttttgacagt
     3421 agataagttg gtagaaaatt taggtagtgt acaagcggtc ttagattctt atcatttatg
     3481 ggcaaataca gaaaaaactt ttcccttgtt agattggttt gttcagcaga aattaattga
     3541 aaaagaaata taaaaactaa aggagtaaac aatgtctaag aaattgacat ttcactgcgt
     3601 cagtggcaga aacctcctta cagtcggact gccccacgct cagcactaga gtgcctgagc
     3661 tagacgcagt actaactcgt cttgcctcgt atgatcgacg aggcagactc atgtcgcaag
     3721 taattatttt ttattaagga gtaaacaatg tctaagaaat taacatttca ctgcatcagt
     3781 ggcagaaacc tccttacagt cggactgccc cacgctcagc accctagtgc tgagctagac
     3841 gcagtactaa ctcgtcttgc ctcgtataat cgacgaggca gactcgtgtc gcaagaaatc
     3901 attttttatt aaggagtaaa caatgtctaa gaaattaaca tttcaagaaa ttattttgac
     3961 tttgcaacaa ttttggaatg accaaggttg tatgcttatg caggcttatg acaatgaaaa
     4021 aggtgcgggg acaatgagtc cttacacttt ccttcgtgct atcggacctg agccatggaa
     4081 tgcagcttat gtagagccat cacgtcgtcc tgctgacggt cgttatgggg aaaaccctaa
     4141 ccgtctctac caacaccacc aattccaggt ggtcatgaag ccttctccat caaatatcca
     4201 agaactttac cttgagtctt tggaaaaatt gggaatcaat cctttggagc acgatattcg
     4261 ttttgttgag gacaactggg aaaacccatc aactggttca gctggtcttg gttgggaagt
     4321 ttggcttgac ggaatggaaa tcactcagtt cacttatttc caacaagtcg gtggattggc
     4381 aactggccct gtgactgcgg aagttaccta tggtttggag cgcttggctt cttacattca
     4441 agaagtagac tctgtctatg atatcgagtg ggctgatggt gtaaaatacg gagaaatctt
     4501 tatccagcct gagtatgagc actcaaaata ttcatttgaa atttcggacc aagaaatgtt
     4561 gcttgaaaac tttgataagt ttgaaaaaga agctggtcgt gcattagaag aaggcttggt
     4621 acaccctgcc tatgactatg ttctcaaatg ttcacatacc tttaatctgc ttgacgcgcg
     4681 tggtgccgta tctgtaacag agcgtgcagg ctatatcgct cgtatccgta acttggcccg
     4741 tgttgtagcc aaaacctttg ttgcagaacg caaacgccta ggttacccac ttttggatga
     4801 agaaacacga gttaaacttt tagcagaaga cgcagaatag tgagtaatac tgtactctaa
     4861 aatcgttaga ggcaagtcga agacttgcta gagggatatg caccgccgta ctgttatgtg
     4921 gggatttttg aaaccttagg ttcaaaaatc agtcagctaa atccactttg atgagattgt
     4981 tggaaatctt gataaattta gcattggatt tccatacaga ctcacagagt tagttagcga
     5041 cgtccccagt acctaaggtg catatcaaaa aagattgaag aaaatgtaaa ggaaaaaaca
     5101 tgacaaaaaa cttattagta gaactcggtc ttgaagaatt accagcctat gttgtcacac
     5161 caagtgaaaa acaactaggc gaaaaaatgg cagccttcct caaggaaaac cgcctgtctt
     5221 ttgaagccat tcaaaccttc tcaacaccac gtcgtttggc tgttcgtgtg actggtttat
     5281 cagacaaaca gtctgatttg acagaagatt tcaagggacc agcaaagaaa attgccttgg
     5341 atagtgatgg aaactttacc aaagcagctc aaggatttgt ccgtggaaaa ggcttgactg
     5401 ttgaagatat cgaattccgt gaaatcaagg gtgaagaata tgtctatgtc actaaggaag
     5461 aagttggtca agcagttgaa gccattgttc ctggtgttgt agatgtcttg aagtcattga
     5521 ctttccctgt cagcatgcac tgggctggaa atagctttga atacattcgt cctgtccaca
     5581 ctttaactgt tctcttggat gagcaagagt ttgacttgga tttccttgat atcaagggaa
     5641 gtcgtgtgag ccgtggccat cgtttcttgg ggaaagaaac caaaattcag tcagcattga
     5701 gctatgaaga agaccttcgt aagcagtttg taatcgcaga tccatgtgaa cgtgagcaaa
     5761 tgattgttga ccaaatcaag gaaattgagg caaaacatgg tgtacgtatc gaaattgatg
     5821 cggatttgct gaatgaagtc ttgaatttgg ttgaataccc aactgccttc atgggaagtt
     5881 ttgatgctaa ataccttgaa gttccagaag aagtcttggt gacttctatg aaggaacacc
     5941 agcgttactt tgttgttcgt gatcaagatg gaaaactctt gccaaacttc atttctgttc
     6001 gtaacggaaa cgcagagcgt ttgaaaaatg tcattaaagg aaatgaaaaa gtcttggtag
     6061 cccgcttgga agacggagaa ttcttctggc gtgaagacca aaaattggtg atttcagatc
     6121 ttgttgaaaa attaaacaat gtcaccttcc atgagaagat tggttccctt cgtgaacaca
     6181 tgattcgtac gggtcaaatc actgtacttt tggcagaaaa agctggtttg tcagtggatg
     6241 aaacagttga ccttgctcgt gcagcagcca tttacaagtt tgacttattg acaggtatgg
     6301 ttggtgaatt tgacgaactc caaggaatta tgggtgaaaa atacaccctt cttgctggtg
     6361 aaactccagc ggtggcagct gctattcgtg aacactacat gcctacatca gctgaaggag
     6421 aacttccaga gagcaaggtc ggcgcagttc tagccattgc agacaaattg gatacgattt
     6481 tgagtttctt ctcagtagga ttgattccat caggttctaa tgacccttat gcccttcgtc
     6541 gtgcaactca aggtgtggtt cgtatcttgg atgcctttgg ttggcacatt gctatggatg
     6601 agctgattga tagcctttat gcattgaaat ttgacagttt gacttatgaa aataaagcag
     6661 aggttatgga ctttatcaag gctcgtgttg ataagatgat gggctctact ccaaaagata
     6721 tcaaggaagc agttcttgca ggttcaaact ttgttgtggc agatatgttg gaagcagcaa
     6781 gtgctctcgt agaagtaagc aaggaagaag attttaaacc atctgttgaa tcactttctc
     6841 gtgcctttaa cctggctgag aaggcagaag gggttgctac agttgattca gaactatttg
     6901 agaatgacca agaaaaagct ttggcagaag cagtagaaac actcgtttta tcaggacctg
     6961 caagtcagca attgaaacaa ctctttgcgc ttagcccagt cattgatgct ttctttgaaa
     7021 atactatggt aatggctgaa gatcaggctg tccgtcaaaa tcgtttggca atcttgtcac
     7081 aactaaccaa gaaagcagct aagtttgctt gttttaacca aattaacact aaataaaatt
     7141 tgataaacgg actttatctt attacaaagg agaagaaatg gatccgaaaa aaattgctcg
     7201 tatcaatgag cttgctaaaa agaaaaaaac agaaggctta acaccagaag aaaaagtgga
     7261 acaagccaaa ctacgtgagg agtacatcga aggttatcgc cgcgctgttc gtcaccacat
     7321 tgaaggaatc aaaattgtgg acgaagaagg aaacgatgtt acaccagaaa aactacgcca
     7381 agtacaacgt gaaaaaggat tacatggccg tagtcttgat gacccaaatt cataatatgt
     7441 actctttctt ttaacaaaga tagatgaaac gataacaaag agactagcag tttgtgtttg
     7501 ctagtctttt ttcgctaaaa aaggaaccat aatggttcct aaaaactat
//
LOCUS       DAVBCG010000092         7397 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBCG010000092 DAVBCG010000000
VERSION     DAVBCG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.092"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            122..277
                     /locus_tag="VQ495_001585"
                     /pseudo
     CDS             122..277
                     /locus_tag="VQ495_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            477..3752
                     /gene="rexB"
                     /locus_tag="VQ495_001586"
     CDS             477..3752
                     /gene="rexB"
                     /locus_tag="VQ495_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2531523.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3749..>7397
                     /gene="addA"
                     /locus_tag="VQ495_001587"
     CDS             3749..>7397
                     /gene="addA"
                     /locus_tag="VQ495_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2531524.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2257 a         1559 c         1581 g         2000 t
ORIGIN      
        1 tagattgaaa taagatacga acagcttgat tagggaaatc aaattaattt ctagaaatat
       61 tttagcatcc acggtgtact attatagttt caatttacta tagttcaatc attctatcaa
      121 gaatattgaa tatataaagg cacgtaatat taaaacttct tctgatatcg aattagagtc
      181 cgatgttgat ggcgataaat cagacaatct accagtcgac attaagatac tgggacaaca
      241 cattgaagtg tattccgtac ctaagaaaca atcataaaac tcaaagcctt gttaccgagg
      301 cttttttatt atgtgatttt cagtagccag acttattcta tctaatacaa gtaaagctaa
      361 gtatttaaga tttagcagat gatgaagaaa agaaaggatg gcctaaatat tttaacagat
      421 ttacgaggct aatcatggta taatataggg tgatgaaaaa ttccaacgag gctgagatga
      481 aattacttta tactgatatt cgaacttctt tgacagaaat tctaacaaga gaggcagaag
      541 agctagttgc agctggcaag cgggtcttct acattgcccc caactctctt tcttttgaaa
      601 aggaacgcgc cgtgctggaa tgcttgtccc agcaggcttc tttttcgatt accgtcacgc
      661 gctttgctca aatggctcgt tatctggtct tgaatgattt accagttaaa actactcttg
      721 atgatatcgg tcttgggttg gccttttaca aatgccttgc cgaactcgat cccaaggact
      781 tgcgtgttta tggcgctatt aagcaggatc ctcaattgat ccagcagtta attgagcttt
      841 accatgagat gaccaaatct cagatgagtt ttttggactt ggagaattta acagatgagg
      901 ataagagggc ggatttactc ttgatttttg agaaagtaac agcctatctt aatcaaggtc
      961 agttagccca gggaagtcag ttgtcccatt tgattgaggc tattgagaat gacaaggtaa
     1021 gtagtgattt taatcaaatc accttggtca ttgacggctt tactcgtttt tctgctgagg
     1081 aagagcgggt tgtggactta cttcacggca aaggtgttga gattgttatc ggggcttatg
     1141 ctagtaagaa agcctatacc agtcctttta gcgagggcaa tctctaccaa gccagcgtaa
     1201 aatttctcca tcatctggct tctaaatacc aaacgcctgc tcaggactgt tctcaaactc
     1261 atgagaagat ggatagtttt gacaaggcct ctcgtttgtt ggagtcttct tatgactttt
     1321 cagaactcgc tttggatgtc gatgagaaag accgtgaaaa tttacaaatc tggtcttgtt
     1381 tgacgcaaaa ggaggagttg gagctagtag cccgtagcat tcgtcagaaa ttacatgaga
     1441 actcagacct gagctacaag cattttcgta ttctcttggg ggatgtagct tcttaccagt
     1501 tatctctcaa aaccattttt gaccagtatc agattccttt ttatcttggt agaagcgaag
     1561 ccatggctca tcatcccttg actcagtttg tcgagtctat tttagcttta aaacgttacc
     1621 gttttcgtca ggaggatttg attaatcttc ttagaactga tttgtatact gacctcagtc
     1681 agtctgatat tgatgctttt gagcaatata tccgctatct tggtatcaat ggcttgccag
     1741 cctttcagca aactttcacc aaatcccacc atggaaaatt taatcttgag cgtttgaatg
     1801 tcctccgcct gagaatttta gcacctcttg aaaccctctt tgccagccga aaacaaaagg
     1861 ctgaaaacct cctacaaaaa tggagtgtct ttctaaaaga aggagctgtg accaagcagt
     1921 tacaagattt gacaaccact ttggaagctg tagaacagga aagacaagcc gaagtttgga
     1981 aggctttctg ccatgtttta gaacaatttg cgactgtttt tgctggttca caggttagtc
     2041 tggaagactt cctagccttg ctccattctg gaatgagttt gtcccaatac cgtaccattc
     2101 cagcaacagt ggacactgtt ctggtgcaga gttacgattt gattgcacca ttgactgctg
     2161 actttgtcta tgctattgga ctaactcagg acaatttacc aaaaatttct caaaacacca
     2221 gtcttctgac agatgaagaa aggcaaaacc taaaccaaac gaccgaagaa ggcgttcaat
     2281 tactgattgc cagcagtgaa aatctcaaga aaaatcgcta cactatgctt tccttggtca
     2341 attctgctcg taagcagttg ttcttgtcgg ctccaagcct ttttaacgaa agtgaaagta
     2401 aggaatctgc ctatcttcaa gagttgatcc attttggatt taggcggaga gagaagagga
     2461 tgaatcacaa aggactgtct aaggaggata tggggtccta tcacagtctt ttgtctagtc
     2521 tggttgccta tcaccagcag ggtgagatga gcgatactga gcaagatttg acttttgtca
     2581 aggttctgtc gcgtgtcata ggtaaaaaac tagatcagca aggtctggaa aatccagcca
     2641 tcccaatcag tccaagcagc aagaccttag ccaaggacac cttgcaagct ctctatcctg
     2701 ccaaacagga gttttacctg tctacgtcgg ggttgacaga gttttatcgc aatgaataca
     2761 gttatttcct acgctacgtt ttaggcttgc aggaggaatt acgtttgcgt cctgatgccc
     2821 gtagtcacgg gaatttcttg catcgtatct ttgaacgcgc cttgcagttg cctaatgaag
     2881 attcctttga ccaacgtcta gaacaagcta ttcaagaaac cagtcaagaa cgcgaatttg
     2941 aagctattta tcaagaaagt ttggaagccc agtttaccaa ggaagttttg cttgatgttg
     3001 cacggacaac tggacatatt ctccgacaca atccagccat cgaaacaatc aaagaagaag
     3061 caaattttgg tggaaaagac caagccttta ttcaattaga caatggacgc agtgtctttg
     3121 tacgaggcaa ggtggaccgg attgaccgtt tgaaagctaa tggagcgata ggagtagtag
     3181 actacaaatc cagtctgact cagttccagt ttcctcattt ctttaatggg ctcaattctc
     3241 agttaccaac ctatcttgct gccctaaaaa gagaagggga gcagaacttt ttcggcgcca
     3301 tgtacttgga aatggctgaa cctgtccaat ctctgatggc ggtaaaaagt ctggcaggag
     3361 cagtggtaga agccagcaaa tctatgaaat accaagggct cttcttggaa aaagaaagca
     3421 gttatttagg cgaattttat aacaaaaaca aggcgaatca actgacagat gaggaatttc
     3481 agctcctact ggactacaat gcctatcttt acaagaaagc tgctgagaag attttagcag
     3541 gccggttcgc catcaatcct tatactgaaa atggcagaag cattgcccca tacgtccagc
     3601 aacatcaggc tattacaggc tttgaagcca attaccatct gggccaagcc cgtttcctag
     3661 aaaagttgga cctagctgat ggcaagcgtc tggtcggaga aaaactcaag caagcttggc
     3721 ttgaaaaaat aagagaggag ttgaatcgat gaagcttatt ccctttttaa gtgaggagga
     3781 gattcaaaaa ctgcaagaag cagaagcaaa ttcgagcaag gaacaaaaga aaactgccga
     3841 gcaaatcgaa gctatctaca cttctggtca gaatatcctg gtctcagcat cggctggttc
     3901 tggaaagacc tttgtcatgg cagagcgcat tctggaccaa ttggcgcgtg gtgtcgaaat
     3961 ttctcaactc tttatctcaa cctttaccgt caaggctgca actgaactta aagaacgttt
     4021 agagaaaaaa atcagcaaga aaatccaaga aacagatgat gtcgacctca aacaacactt
     4081 gggacgccag ttggcagacc taccaaacgc tgccatcgga accatggact ctttcacaca
     4141 aaaattcctt ggcaaacatg gttatctgct tgatattgca cctaatttcc gtattttaca
     4201 aaaccaaagc gagcaacttc ttctcgaaaa cgaagtcttt catgaggtct ttgaagcgca
     4261 ttaccaaggt aaacagaaag agacctttag tcatttgctg aaaaactttg ctgggcgtgg
     4321 caaggacgaa cggggtctgc gccagcaggt ctataaaatc tatgacttcc tccaatccac
     4381 cagtaatcct caaaagtggc tgagtgaatc tttcctcaaa ggatttgaga aagctgattt
     4441 taccagtgaa aaagaaaaac tgactgagca aatcaaacaa gccctttggg atttggaaag
     4501 ctttttccgt taccatctgg ataacgatgc caaggagttt gcaaaggctg cctatttaga
     4561 aaatgttcag ttaattctgg atgaaatttg ctccctaaat caggagtccg atagtcaggc
     4621 ttatcaggca gtgcttgcgc gtgttgtcgc catctctaag gagaaaaacg gtcgagctct
     4681 gactaatgcc agtcgtaagg ctgatttgaa gcccctggct gatgcctaca acgaagagag
     4741 aaagacccag tttgctaaac taggacaatt atcagaccag ataaccattc tcgactatca
     4801 agaacgttat catcaagaca catggaaact agctaaaacc ttccaaacct ttatgagtca
     4861 ttttgtggag gcttatcgtc agcgcaaacg ccaggaaaat gcctttgaat tcgctgatat
     4921 cagccattac accattgaga ttttagagaa tttcccacaa gttcgtgagt cttatcagga
     4981 gcgcttccat gaagtcatgg tcgatgagta tcaggatacc aaccatattc aagaacggat
     5041 gctggaattg ttgtctaatg gccacaatcg ctttatggtg ggagatatca agcaatccat
     5101 ctatcgtttc cgtcaggcag acccgcagat tttcaatgag aaattccaac gctatgcgca
     5161 aaatccccaa gaaggcaagc tcattctcct caaggaaaat ttccgtagta gttcagaagt
     5221 gctgtcagca accaatgatg tctttgaacg tctcatggac caagaggtcg gtgaaatcaa
     5281 ctatgataac aagcaccagc ttgtttttgc caataccaaa ctgactccca atccagacaa
     5341 caaggcagaa tttctcctct acgacaagga cgatacaggt gaggaagaag agagtcaaac
     5401 agaaacgaaa ctaacaggcg aaatgcgctt agttatcaag gagattctga aacttcatca
     5461 agaaaaaggt gttgccttta aggaaattgc ccttctgacc tccagccgca gtcgtaatga
     5521 ccagattctc ctcgccctgt ctgagtacgg gattcctgtc aaaactgacg gagagcaaaa
     5581 caattatctc caatccctag aagtgcaagt catgctagac actcttcgtg tcattcacaa
     5641 tcccctgcaa gactacgcct tggttgccct tatgaagtct ccaatgtttg gttttgatga
     5701 ggatgagcta gcacgtttgt cccttcagaa agcagaggat aaagtccacg aaaatctcta
     5761 tgagaaactg gtcaatgcac aaaaaatggc aagtagtcaa aaaggcttga ttcacacagc
     5821 tctagttgaa aaactaaagc aattcatgga tatcctagct tcttggcgct tgtatgccaa
     5881 aacccactct ctctatgact tgatttggaa gatttacaac gaccgttttt attatgacta
     5941 tgttggggct ttgccgaatg gtcctgctag gcaggccaat ctctatgccc tagcactgcg
     6001 tgctgatcaa tttgaaaaga gcaatttcaa aggtttgtcg cgttttattc gtatgattga
     6061 ccaagtctta gaagcccagc acgatttggc aagcgtggcc gtcgcaccgc caaaagatgc
     6121 agtagagctc atgaccatcc acaagagtaa agggctggag tttccttacg tctttatcct
     6181 caatatggat caagatttca acaagcaaga ctctatgtca gaagtcattc tcagtcgtca
     6241 gaatggtctt ggtgtcaaat atattgccaa gatggagaca ggggcagtag aagaccacta
     6301 tcctaaaacc atcaaactct ccattcctag tctgacctat aggcagaacg aagaggaatt
     6361 acagctagca agctattctg agcagatgcg tttgctgtat gttgctatga cgcgggctga
     6421 gaaaaagctc tatcttgtcg gcaagggttc tcgtgaaaag ctggaatcca aggaataccc
     6481 agcagccaaa aatgggaaac taaatagcaa tactagactg caagcacgga atttccaaga
     6541 ttggctttgg gctatcagta aagtgtttac taaggacaag ctcaacttta gttatcgttt
     6601 tattgacgaa gatcagttga ccagagaagc tatcggagag ttggaaacca agagtcctct
     6661 ccaagatagc tcccaagcag acaatcgtca gtcagatacc atcaaagaag ctctggaaat
     6721 gctgaaggag gtggaagttt ataatactct tcaccgcgca gctattgaac ttcctagtgt
     6781 tcaaacccca agtcaaatca agaaattcta cgaaccagtt atggatatgg aaggtgtcga
     6841 gattgctggt caaggtcagt cagtaggcaa gaaaatcagc ttcgatttgc cagatttttc
     6901 aaccaaagaa aaggtaactg gagctgagat tggtagtgct actcacgaac tcatgcagat
     6961 aattgacctc agccagcaac taacccttgc tagcttaaca gaaacactca aacaagttca
     7021 aactagccaa gctgtcagag acaagatcaa tcttgataaa attcttgctt tctttgacac
     7081 agcactcggt caggaaattc ttgctaatac cgaccatctc tatcgcgagc aacctttctc
     7141 catgctcaaa cgagaccaaa agagtcagga agactttgtt gtccgtggta tccttgatgg
     7201 ctatctgctt tacgaaaaca aaattgttct gttcgactac aagacagacc gctatgatga
     7261 accaagtcaa ctcgtagacc gctatcgtgg tcagttagct ctatacgaag aggctttatc
     7321 acgagcctat tcgattgaaa atattgaaaa atacttgatt ttactcggta aagacgaggt
     7381 tcaagttgta aaagtat
//
LOCUS       DAVBCG010000093         7316 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBCG010000093 DAVBCG010000000
VERSION     DAVBCG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.093"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(1..831)
                     /locus_tag="VQ495_001588"
                     /pseudo
     CDS             complement(1..831)
                     /locus_tag="VQ495_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(845..1711)
                     /locus_tag="VQ495_001589"
     CDS             complement(845..1711)
                     /locus_tag="VQ495_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2531525.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(1725..2984)
                     /locus_tag="VQ495_001590"
     CDS             complement(1725..2984)
                     /locus_tag="VQ495_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2531526.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(3076..5238)
                     /locus_tag="VQ495_001591"
     CDS             complement(3076..5238)
                     /locus_tag="VQ495_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2531527.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            5346..6206
                     /locus_tag="VQ495_001592"
     CDS             5346..6206
                     /locus_tag="VQ495_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2531528.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            6203..7138
                     /locus_tag="VQ495_001593"
     CDS             6203..7138
                     /locus_tag="VQ495_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2531529.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
BASE COUNT         2325 a         1524 c         1321 g         2146 t
ORIGIN      
        1 ttcactgccc cgttgcttat tcctgcgatg atatggcgtt ggaagaagag atagacaatg
       61 gtgatactga taatgccgac cacgtaagag gcaaagcttg gtccgtagtc gttgaaatat
      121 tggcctgcgt agttgtattg gaacaaaggc agagtccaca ttttggaatc ccggttcaag
      181 acaaggagtg gcaacatgaa gtcattccag aaccaaaggg cattgatgat catggttgtc
      241 gcatgcatcg gtttcatcat tgggaagatg atgcggaaat aggttgtaaa ttgattagcc
      301 ccatcgatct ctgctgcttc atccagactt tctggaatcg agattttgat atagccaaca
      361 tagagaaaga gggtctgtgg aatcgcatag gtcaagtaga gcaagatcaa accaaaggta
      421 ttagccaaac cgagtttact catcataacc gtaatcggaa tcatgatgac ttggaaaggt
      481 acgaagattc cgaggattaa gagggtatac atgatggtaa aggcttttct tttactcata
      541 ttgcgagcga tggagtaggc tgccataggg ataaagatca ttactgcaag taaagacaag
      601 acagtgatga cgacagagtt ccaataatag cctccaatcc ccatcagcta agagacggct
      661 aaagttgtcc catgtgaagt tggttggaaa gccaaagaaa ttatctacaa tatccttagt
      721 ggatttgaag gaactaaaga gggtagcaag gagcggcact aaaatcagaa ccgatcctag
      781 aatcaataga atgtatttgc caatcagggc ttttctttca tcttgtttca tcatgcttct
      841 cctcttaaat ttcaaatttc ttagatactc tcaattggat gatcgaaatc actacaatta
      901 agaagaacaa gattacggca atggcattgg cataaccgaa ttggttgttt ttaaaggcat
      961 agttataaac caagagccca agtgaggttg tggcattgtt tggaccacca ccggtcatgg
     1021 caaagacttg gtcaaaggca gtcagcccac cttttagggc taggataaag actatagaga
     1081 cacttggtag caagtaaggc aattcaatgt tccagaaaac ttgcttgcta gtcgcaccat
     1141 caatccttgc tgcctctgta atctcagttg gaatagattg caaaccagct aggaagatga
     1201 tgatgggcat agccacccct tgccaaagaa ggacaaagac agccgcaaag attgctcccc
     1261 acttagtccc taaaagactg gtttggagaa attcaatatg aagggcattc ccaatcgctg
     1321 gaagaccgta gttgaagact tgcttgaaga tcaaagccac tgtcaaacca gataaaacag
     1381 ctgggaagaa gaaccaagca cggaagaagg tttggccttt gattttagag ttcaagacac
     1441 gcgcaatgaa gatcccgagt gcaatctcac caaccaccat ggcaatcgta atgattgcgg
     1501 taaagccaat cgcattcatg aattttggat ccatgaagag gagcttaaag ttgtttaagc
     1561 cgacaaattt gtagttgtaa gtcaatcctg tccagttggt aaaactgtaa aaggctcctt
     1621 gaaacatcgg cacatagaag aaaattgctt gtaacaagag ggggatgacc acaaaagccc
     1681 atgcccaata tttttgtaat acttttttca tagtctctct actcctaatc cacatccgct
     1741 ttcatcgggt taaagaaggc attcaaatca ttgaccatgc cttgtttatc accggtcaag
     1801 acatagttca tggtcaaggt atggaagtct gcttcactgg tccagtattg ttgcaaccag
     1861 accaagtgac gatccgtaaa ggcatattcg gtcataccag caagcggtga atcttctcct
     1921 gcttgtttga ccccttcgat cgctgttgga gatccgtcca catcgtagta tttttgcatg
     1981 acttctgaac gggtcatata ttccacaaag gcattggctt cttttggatg tttggtggtg
     2041 gctgagatag accatgccaa gtctcccgca ccaacggtta agctttgtcc tttttctttt
     2101 cctggaatca tgaaggttcc aatcttaaag ttcggttttt gttcattaat cgctgtgatc
     2161 gcccaagacc catttggtgt catgaggaca tccccacgtg cgaaggctcc gataacatcg
     2221 gtatagccag caccttccca gttcttttgc ttagagccat taatgcgaag gatgtccatg
     2281 accttgatat catctttcat aatcggatcc gacaatttaa tagcatttgg ttgagaataa
     2341 cgaaggtatt gatttgcttc ttttcctcca cctgttgctg tcgcaaaggc taattgattg
     2401 taaccattga gtgtccaagc atctgcacct gcaattccaa atggtgtttg tcctttagca
     2461 acgatatctt tgactaactg ttcaaattca tcccaggttt caggaacctt caagcccagt
     2521 tcttcgaatt tatctttgtt gtagtaaatt ccataagcat tagctgtaaa aggaacgttg
     2581 taaacttttt cgtttacagc atatttttca gcgtagccat ttttcacgcg tttcaggtag
     2641 tctttgttac tcaaatcttc aaagacacct gcttttgccc attcttgcag ttcgatggac
     2701 tgtgggtaaa tattgaccac atcaggcaca tctcctgcga gaacgcgtgt cttcaatact
     2761 tcaccagcat ttggtacatt gacgactttg acctttatct tagggttttc cttctcaaaa
     2821 tcacgagtga tttcttccaa ggttttggtc atttcttttt tctggttgaa atactcgatg
     2881 gtcactgtgt catccgcaga tttaccatag ttggagcaag caccgagccc aaacaaagct
     2941 aaacctgtag ttgcaagaag tccgattttt ttataccatt ccattagaaa gcctccttta
     3001 taaatttata cacccttatc aaactgcact tcaaaagtta gatagaataa atctaacttt
     3061 tggggtcagt acatatcata gttttctaaa aatatactgt ctactcaaaa aatctccttg
     3121 ggataagata acagttaagc ccgcatacat tagttctgca cctgagtaaa cttcgccatt
     3181 ttcctgtaat ttatatagtc cctcttcatc caaatctttt aattttaaag ttgtttccat
     3241 ggtctctaca acagataaaa cgcgaacgta ggttacaatc gtttgatttc cgtaattaaa
     3301 ttgtacagct gcttcattgg atgcagtatc aggattaatt agtctatact gctgtcctaa
     3361 ctgaactact ggtcgtaatt ctttatacaa gttcacctga ttagcaatcg tagctttctc
     3421 ttcatctgat aaatttgtca aatcaagctc atagcccaaa tttcccatca ttgctacatg
     3481 gccacgtgtt tctaatggtg tcattcgtcc catctgatga ttcggtactg ctgacacatg
     3541 agcccccata gaaatggttg gatagaaata ggatgaaccg tattgaattg gtaaacgtgc
     3601 aatggcatca gtattatcac tagcccagac ttgtgggaaa tagcgtatca taccaagatc
     3661 atttcgtcca ccaccaccag agcaggactc aaagagaata tggctgtgct tctctgtcag
     3721 ataagaaacg agttcataaa gccccagcat gtactgatga gattgcatct gtgtctctag
     3781 ataagttaat ccattcccta gcttagtgat attgcggttc atatcccatt taatgtaatc
     3841 aatctcatga taagatagga gttgatctaa gacacttttc aagtattcta ctacctgagg
     3901 attggcaaga ttaagtacta attgattccg agaataagta tgctcatagt caggaacctg
     3961 aatagcccag tcagggtgtt gacgatacaa atcactatct acagaaatca tttcgggttc
     4021 taaccaaagt ccaaactgca aacctctttc atggatagct gaaatcagac tttctagact
     4081 tccacccagt ttttcctcat taacaaccca atcacctaaa gcacgattat catcaaaacg
     4141 attgccaaac caaccatcat ctaatacaaa aagttcaatg ccaactttct tagcttcatc
     4201 tgctaactct aacagttttt ctctctgaaa gtcaaagtaa gtagcttccc agttattgat
     4261 tagaattgga cgttcttttt tagaaaattc acttagaata atgtgcttca gtacaaaatt
     4321 ctgactttca tgactaatac cagttaatcc ctgatctgaa tgagtcacta aagctaccgg
     4381 tgtttcaaag tattcctcag gggctaactt ccaagaaaag ttttctggat taatgccaat
     4441 agccacccga acttcattca attgattttt ttgaacaaaa gcttcaaagt tgccactata
     4501 cattagttga atagcaaaca cattcccagc atcctctgtg actccttgtt cgcatagtag
     4561 aagagctggt gtttgagcat gaccagaagc acctcggttt gaactaatcg aaaagattcc
     4621 ttgttctacc tgttgacgtc taacagtctt ttcacgagca taagcaccct gcagagttgc
     4681 tatttcgtaa tctgcagctg gaaaatcagc cataaaagaa aaatctttat ggatgacaac
     4741 ttcctgatta ctattattat ctaatttact gtagctagta atagtcgcat cattattaaa
     4801 agtagtataa tacaaagtca gactaagttg agccttagaa tcttctaaca ttaagacaag
     4861 agtctctgta tcgtccatgc tatgtggaga aggtaagccc tgtggaccat tctgaccttt
     4921 taaaatcttt gcttctacaa atcgaaagtc tgttacttca gttacactat gctgaacctg
     4981 tatagttggt ttcctaaaat ctcctaagcc atgttgtcca aaaatctgtc gctgagtatc
     5041 taaactaaag gttcgattag tagccgttgg atttcctgaa aaggcatggt ctcgttcata
     5101 aacactattg gaacctttat agttcttaat agtctttcct aaatgtttca aaagtaagta
     5161 gccatttcga ttttcaataa tcaaacttag atttttactc tcaacataaa atagattatt
     5221 ctctatccta actcccattt acttcacctc atcactttat tgattatatt ttatcacctg
     5281 aaatcgcttt ccaaaataga aaaatgtctc aagaatatgg taaaatgtta ggtaggaggt
     5341 agcacatgtt agttttttca gaataccaga ctggaacaat cgaccttgcc ctaagctttt
     5401 atggatatga ggaatgcaca cctaattact cttttggtcc agccattcgt gatacatacg
     5461 ttctacatta cattactaaa ggacaaggaa aattttatta caagggtaaa attgttgatt
     5521 taaaagaagg agatttcttt ctattaaaac cagaggaact aaccttttat caagcagata
     5581 gtaaagaacc ttgggcctac tactggttag gaatcactgg agggaaatcc cctgattatt
     5641 ttgctctttc tcaaatttct gatcaatcct atctcatcca atctgaaact tgtcataccc
     5701 agactactgc aaaactcatc tcagacattg tccgcttcgc tcagattaca aaatcaagtg
     5761 aattagctca actccatatc atgggacaac ttcatgaact gatgtttcat ctgggaacta
     5821 ttgctcccaa tcagaaaaaa aagaatattt catcaaccca ccaactctat cttgaatgca
     5881 aacgattaat tgatagccac tatcctcaat cacttacaat tcaagattta gcaaaagaac
     5941 tatccgttca cagaagctac ttatcaagcg tattcaaaga atttaatacc ttatcaccca
     6001 aagaatacct actctacgtt cgaatgcacc gagctagaca acttctcgaa aatacccaag
     6061 agtccatcaa ggtaattgca tactcggtag gtttttcaga tccactccat ttttcgaaag
     6121 cttataaaca atactttaat cagactccaa gtcatacaag aaaagaatac tctcaatacc
     6181 aactagtgag aaaggcaaca ttatgaaatc ctaccaagct gtctaccaaa tcctatctaa
     6241 agaaaccgac tatatcagcg gagaaaaaat cgcagaaaaa ctatccctaa gccgaacagc
     6301 aatttggaaa gccatcaagc gactagaaca agaaggcatt gaaattgata gtatcaaaaa
     6361 tagaggatat aaactgatga atggtgacct tattcttcca gagattctag aagaaaatct
     6421 tccaattaaa gtcagcttta aacccgaaac aaaatcaaca caactagatg caaaagaagc
     6481 aattgattta ggccatgaag caaataccct ctatctagct tcctatcaaa cagcaggccg
     6541 aggccgtttt caacgttcct tctactcacc acaaggtggt atttatatga cactccatct
     6601 taaaccaaat ctcccctatg acaaattacc atcctacaca ctacttgtag ctggagctgt
     6661 ctacaaagcc attaagaacc taactttaat agatgtcgac ataaaatggg tcaatgatat
     6721 ctatctaaac aatcataaaa ttggaggaat ccttactgaa gcaatgacct ctgtagaaac
     6781 tggcttagtc acagatatca ttattggagt aggtatcaat ttcactatta aagacttccc
     6841 tcaggaatta aaagaaaaag ctgccagctt atttaaagct acagctccta taacaaggaa
     6901 tgaattgatc atagaaatct ggcgtgcttt cttcgaaaca ccagcagaag agctattata
     6961 cctatacaaa aaacagtcat tcattctagg aaaagaagtc actttcacac tagagcaaaa
     7021 agactacaag ggacttgcta aagacatctc agaaaatgga aaacttttag ttcaatgtga
     7081 taacggaaaa gaaatctggc taaatagtgg cgaaatttct ctcaatagtt ggaagtaaaa
     7141 taacacaatt ataatataaa cgatataaaa ataacttcag attagtaatt caattaagtt
     7201 ttacggatct gaagttttat tgtctctaaa aacaaaaaag agagttacaa actctcatta
     7261 aaacgggctc tataatattt gtagtgggta aattccctat ggatattatg gagcct
//
LOCUS       DAVBCG010000094         7314 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBCG010000094 DAVBCG010000000
VERSION     DAVBCG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.094"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            56..925
                     /locus_tag="VQ495_001594"
     CDS             56..925
                     /locus_tag="VQ495_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2531530.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            929..1885
                     /locus_tag="VQ495_001595"
     CDS             929..1885
                     /locus_tag="VQ495_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2531531.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1885..2649
                     /locus_tag="VQ495_001596"
     CDS             1885..2649
                     /locus_tag="VQ495_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531532.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2649..3359
                     /locus_tag="VQ495_001597"
     CDS             2649..3359
                     /locus_tag="VQ495_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531533.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3667..4323
                     /locus_tag="VQ495_001598"
     CDS             3667..4323
                     /locus_tag="VQ495_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2531534.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            4431..5526
                     /gene="prfB"
                     /locus_tag="VQ495_001599"
     CDS             join(4431..4502,4504..5526)
                     /gene="prfB"
                     /locus_tag="VQ495_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2531535.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5544..6236
                     /gene="ftsE"
                     /locus_tag="VQ495_001600"
     CDS             5544..6236
                     /gene="ftsE"
                     /locus_tag="VQ495_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2531536.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6229..7155
                     /locus_tag="VQ495_001601"
     CDS             6229..7155
                     /locus_tag="VQ495_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531537.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         2156 a         1266 c         1641 g         2251 t
ORIGIN      
        1 cctatcaaaa agtgagggaa aaccctcgga attataaata gaaagagtga atcttatgct
       61 ccaacaactc gtaaatggtt tgattctagg tagtgtttac gcgctgttag ccctaggata
      121 taccatggtt tacggaatta tcaagctcat caacttcgcc catggtgata tttatatgat
      181 gggagccttt atcggttatt tcttgatcaa ttctttccaa atgaatttct ttgtagcgct
      241 tattgtagct atgctagcga cagctattct tggtgtcgtg attgagtttc ttgcttaccg
      301 acctttgcgc cactctactc gtattgctgt tttgattacg gctattgggg tttctttcct
      361 attggagtat ggaatggtct atctggttgg tgccaatacc cgtgccttcc ctcaagcgat
      421 tcaaacagtt cgatatgatt tgggaccaat tagcttaaca aatgtgcagt taatgatttt
      481 ggccatttcc ttgattttga tgattttgtt acaagtcatt gtccaaaaga ctaagatggg
      541 gaaagccatg cgtgcagtat cagtagatag cgacgcggcg caattgatgg ggatcaatgt
      601 aaaccgtacg attagcttta ccttcgcttt gggttctgct cttgcgggtg cggctggtgt
      661 tctgattgct ctttattata actctcttga gcctttgatg ggggttactc caggtcttaa
      721 atctttcgtt gccgcagtac ttggtggtat cggaattatt cctggtgcgg ctcttggtgg
      781 ctttgtgatt ggtctattgg aaacctttgc gactgccttt gggatgtcag atttccgtga
      841 tgccattgtt tatggaatct tgttgttgat cttgattgtc cgcccagctg gtatccttgg
      901 taagaatgtg aaagagaagg tgtaaacgat gaaggaaaat ttaaaagtta atattctatg
      961 gttactcctt ttgttagctg gctatagctt gattagtgta ctggtttcag tcggagtact
     1021 taatctattc tatgtacaga ttttacaaca aattggaatt aatattattt tggctgttgg
     1081 tctcaactta atcgttggtt tttcaggaca attttcactt ggtcatgctg gtttcatggc
     1141 gattggtgcc tatgcagcag ctattattgg ttctaaatta ccaacctacg gtgccttctt
     1201 tggagctatg cttgtagggg ctttgctttc aggagcagtt gccttacttg tcggcattcc
     1261 aaccttgcgc ttgaaggggg actatcttgc ggtagcaact ctaggtgttt ctgaaattat
     1321 ccgtatcttt atcatcaatg gtggaagtct tacaaatggt gcggcaggta tcttagggat
     1381 tcctaacttt acaacttggc aaatggttta cttctttgtc gtgattacaa ccattgcaac
     1441 cttgaacttc ttgcgtagcc caattggccg ttcaaccctc tctgttcgtg aagatgaaat
     1501 cgctgctgag tcagttgggg ttaatacgac taaaattaaa atcatcgctt ttgtctttgg
     1561 tgccattact gcaagtattg ctgggtcact tcaggcagga tttatcgggt ctgttgtacc
     1621 gaaagattac accttcatta actcaatcaa cgttttgatt attgttgtat ttggtggact
     1681 cggttccatt acaggtgcga ttgtttcggc tattgttctg ggaattttga atatgcttct
     1741 ccaagatgtt gctagtgtgc gtatgattat ttacgcttta gccttggtat tggtaatgat
     1801 tttcagacca ggtggactcc ttggaacgtg ggaactgagc ctatcacgtt tctttaaaaa
     1861 atctaagaag gaggaacaaa actaatggca ttacttgaag taaaacagtt aaccaaacat
     1921 tttggtggtc taacagctgt tggagatgtg actcttgaat tgaacgaagg ggaactggtt
     1981 ggattaatcg gtccaaacgg agctgggaaa accacccttt tcaacctttt gaccggtgtt
     2041 tatgaaccaa gcgagggaac agtaacccta gatggtcacc ttttgaatgg gaaatcacct
     2101 tataagattg cctctttggg acttggacgt actttccaaa atatccgtct ctttaaagat
     2161 ttaacagttt tagataatgt tttgattgct tttggaaacc atcacaaaca gcatgttttt
     2221 actagtttct tacgcttacc agctttttac aagagtgaaa aagaattaaa ggctaaagct
     2281 ttggaattgt tgaaaatctt tgatttagat ggtgatgcag agactcttgc taaaaatctt
     2341 tcctacggac aacaacgtcg tttggaaatt gttcgtgccc ttgctacgga acctaaaatt
     2401 ctcttcttag atgaaccagc agcaggtatg aacccacagg aaacagccga attgactgag
     2461 ttaattcgtc gtatcaaaga tgaatttaag attacaatca tgttgattga acacgatatg
     2521 aatctggtca tggaagtaac agaacgtatc tacgtacttg aatatggccg tttaattgct
     2581 caaggaactc cagacgaaat taagaccaat aaacgcgtta tcgaagctta tctaggaggt
     2641 gaagcctaat gtctatgtta aaagttgaaa atctttctgt gcattacggt atgatccaag
     2701 cagtccgtga tgtaagcttt gaagttaatg aaggagaagt tgtttccctt atcggtgcca
     2761 acggtgcagg taagacaact attcttcgca ccttgtcagg tttggttcga ccaagttcag
     2821 gaaagattga atttttaggt caagaaatcc aaaaaatacc agctcagaaa atcgtggcag
     2881 gtggtctttc acaagttcca gaaggacgcc acgtctttcc tggcttgact gttatggaaa
     2941 atcttgaaat gggagctttc ttaaagaaaa atcgtgaaga aaatcaagct aacttgaaga
     3001 aggttttctc acgctttcct cgtcttgaag aacgtaagaa ccaagatgca gctactcttt
     3061 caggagggga acaacaaatg cttgccatgg gacgcgctct tatgtcaaca ccaaaacttc
     3121 ttcttttaga tgaaccatca atgggacttg ccccaatctt catccaagag atttttgata
     3181 tcattcaaga tattcagaag caaggaacaa ccgtcctctt gattgaacaa aatgccaata
     3241 aagcacttgc aatctctgac cgaggatatg tattggaaac aggaaaaatc gtcctatcag
     3301 gaacaggaaa agaactcgct tcatcagaag aagtcagaaa agcatatcta ggtggctaaa
     3361 acaatccagt ggattgtttt agtcggcaga tgaagattac gaagtaatca tcaatatagt
     3421 ccgggggccc tttttagtcg gtagattgag attgcaaaca aatctgcatc tacattgaaa
     3481 gcttaatttc taataattga aaaaatcgaa tgaaaagttt cttaccttca ttcacagagc
     3541 tcgattttag agctcttttt gctagcttat tcatactttt ctaaattttg aaaaagaaat
     3601 gtaagcgttt gatagatttg caaaaagatt gtataatagg gataagaata gaaaaggaga
     3661 agtctcatgg cagttaaaga ttttatgacc cgcaaggtag tttatattag tccagataca
     3721 acagtatctc atgcagcaga tttgatgaga gagcaaggtt tgcaccgtct gcctgttatc
     3781 gaaaatgatc aattggttgg tttggtgact gagggaacca ttgcacaagc aagtccatct
     3841 aaagcaacaa gtctttctat ctatgagatg aattatcttc tgaataagac aaaagtaaaa
     3901 gatgtcatga ttcgcgatgt tgtcactgtc tcaggctatg ctagtctaga agatgcaact
     3961 tatctgatgt tgaaaaataa gattggtatt ctccctgtcg tagataacca tcaagtatac
     4021 ggagttatta ctgaccgtga cgttttccaa gcctttcttg aaattgcagg ttatggcgaa
     4081 gaagggattc gtgtacgctt tgttacagaa gatgaagttg gtgttcttgg aaaaattgtt
     4141 tctttgattg tagaagaaaa tttgaatatc tcccatacag tcaatattcc gcgtaaggat
     4201 ggtaaggtga ttatcgaagt gcaaatcgat gaatcaattg atttaccagc cttgagagaa
     4261 aaatttgaag caaatggtat tcaagtggaa gaaatcgctc gcacttcagc aaaagtcttg
     4321 taagaaggga agtccaaagg cttctttttt catgaaaagg ggattagagc aaaagatgga
     4381 aagaaatgat aaaatatgct ataatgaaat aatgtaaaaa aggagtattt atggacattt
     4441 cagtaattcg tcagaaaatt gacgcaaatc gtgaaaaatt agcttctttc agggggtctc
     4501 tttgacctcg aagggctaga ggaagagatt gccatcttgg aaaacaagat gacagaacct
     4561 gatttttgga acgataatat tgcggcccaa aaaacgtcgc aagaattgaa tgaattaaaa
     4621 aacacttaca ataccttcca taagatggaa gagttgcagg atgaagtcga aattttattg
     4681 gattttttgg ctgaagacga gtcagtgcat gatgaactgg tagcgcagtt agccgaactt
     4741 gataagataa tgaccagcta cgagatgact ctactcttgt cagaacctta tgaccacaac
     4801 aatgccatct tggaaatcca tccaggttct ggtggtactg aggcgcagga ctggggtgat
     4861 atgttgcttc gtatgtatac tcgttatggt aatgctaaag gctttaaagt ggaagtgttg
     4921 gattaccaag caggtgatga ggctggtatt aagtcggtaa ctttatcatt tgaagggcct
     4981 aatgcctatg gtctcctcaa gtcagaaatg ggtgttcacc gcttagtgcg aatctcacca
     5041 tttgactctg ccaaacgtcg ccatacctct ttcacatctg tagaagtgat gccagaattg
     5101 gatgatacta ttgaagtgga aatccgtgaa gatgatatca agatggatac cttccgttca
     5161 ggtggtgccg gtggacaaaa cgtcaataag gtttcaacag gtgtacgttt aacccacatt
     5221 ccaactggaa ttgttgtcca atcaacagtg gatcgtaccc agtatggaaa tagagatcgt
     5281 gccatgaaga tgttgcaggc taagctctat caaatggagc aagataagaa ggctgcggag
     5341 gtagattctc tcaaaggtga gaaaaaggag atcacttggg gaagccaaat ccgttcttat
     5401 gtcttcacgc cttatactat ggtaaaagat caccgaacta gctttgaggt tgctcaggta
     5461 gataaggtta tggatgggga cctagatggt tttatagatg cttatctcaa gtggcgaatt
     5521 agctaagata gaaaggaact cacatgtcaa ttattgaaat gagagatgtc gttaaaaaat
     5581 acgacaacgg aacgactgct ctacgcggtg tttcggtcag cgttcaaccg ggggaatttg
     5641 cttacatcgt aggaccttca ggagcaggga agtcaacttt tattcgttct ctgtatcgtg
     5701 aagtaaaaat cgataaagga agtctatcag ttgctggttt taatctggtt aagatcaaaa
     5761 agaaagatgt cccgcttcta cgtcgtagtg ttggggttgt cttccaagat tataaattgt
     5821 taccaaagaa aactgtctat gaaaatattg cttacgctat ggaagtaatc ggggaaaatc
     5881 gccgtaatat caaaagacga gtgatggaag ttttggactt ggttggattg aagcataagg
     5941 ttcgttcttt cccaaatgaa ctctcaggtg gggagcagca gcgaattgcg attgcgcgtg
     6001 caattgtaaa taatcccaaa gtattgatag ctgatgagcc aacaggaaat ctggatccgg
     6061 ataattcatg ggaaattatg aatctcttgg aacggattaa cctacaaggg acaactattt
     6121 tgatggcgac tcataatagc cagattgtaa ataccttgcg ccaccgtgtc attgccattg
     6181 aaaatggccg tgtcgttcgt gacgaatcaa aaggagagta tggatacgat gattagtaga
     6241 ttttttcgcc atttatttga agccttaaaa agtttgaaac gaaatggttg gatgacagta
     6301 gctgctgtca gttcagtcat gattactttg accttggtgg caatatttgc atctgttatt
     6361 ttcaatacag cgaaactagc tacagatatt gaaaataatg tccgtgtagt agtttatatc
     6421 cgaaaggatg tggaagataa tagtcagaca attgaaaaag aaggtcaaac tgttacaaat
     6481 aatgactacc acaaggtata tgattctttg aagaacatgt ctacggttaa aagtgttacc
     6541 ttttcaagta aagaagaaca atatgaaaaa ttaaccgaga taatgggaga taactggaaa
     6601 atctttgaag gagatgccaa tcctctctat gatgcctata ttgtagaggc aaacactcca
     6661 aatgatgtaa aaactatagc cgaagatgct aaaaaaattg aaggtgtctc tgaggttcaa
     6721 gatggcggtg ccaatacaga aagactcttc aagttagctt catttatccg tgtttgggga
     6781 ctagggattg ctgctttgtt aatttttatc gcagttttct tgatttcaaa taccattcgt
     6841 attaccatta tttcccgcag tcgcgaaatt caaatcatgc gcttggttgg agctaaaaac
     6901 agttatatcc gtggaccgtt cttgttagaa ggagccttta tcggtttatt gggagctatc
     6961 gcaccatctg ttttggtctt tattgtttat caaattgttt accaatctgt caacaaatcg
     7021 ttggtagggc aaaatctatc catgattagt ccagatttat ttagtccgtt gatgattgcc
     7081 ctactatttg tgattggggt tttcattggt tcattgggat caggaatatc catgcgccga
     7141 ttcttgaaga tttaggtaaa atagctgctt ttatgaggag attgtaaaat ctcctttttt
     7201 gctacaagag tttttgaaaa gagatgcgta gaagaaaaga gcttccaaag aagtccccca
     7261 gagaagactt cttgatggct aatttgaact tgaaatttgg cgcactagaa attt
//
LOCUS       DAVBCG010000095         6993 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBCG010000095 DAVBCG010000000
VERSION     DAVBCG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.095"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            145..6831
                     /locus_tag="VQ495_001602"
     CDS             145..6831
                     /locus_tag="VQ495_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2531538.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTVEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQTSNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGFKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEDTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
BASE COUNT         2306 a         1346 c         1569 g         1772 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgttgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caaacttcaa atcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ttttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgtagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggttttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaaga tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtcaaac taataaagcc
     6721 cagttaccaa atacaggtag tgcggcaagc caagcagcag tagcagcagg tttagctctt
     6781 ctaggtttga gtgcaggatt agtagttact aaaggtaaaa aagaagacta gaaattttga
     6841 taccttcttt atcattaaag accaaagaaa aactcttgtc ctaaatagaa gataaagagt
     6901 ctggaacaaa atagttctca accacaaaaa agctagatat ttccaattgc ggaactctag
     6961 ctttttaatt ttgagtcgtt ctcttattgt ata
//
LOCUS       DAVBCG010000096         6988 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBCG010000096 DAVBCG010000000
VERSION     DAVBCG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.096"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            225..6656
                     /locus_tag="VQ495_001603"
     CDS             225..6656
                     /locus_tag="VQ495_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2531539.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPYGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAY
                     DDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
BASE COUNT         2759 a          943 c         1347 g         1939 t
ORIGIN      
        1 aatcttattt tttaacgtgt gctaaaaaat aaattaatgc atgttaattt ttcttgaatt
       61 aaattttttt aaatgatata ctgtttttat ggagagataa caattatata taaatgtact
      121 actctatttc ctagataata cttagtaaaa ctttttataa caaaggctag caaagttaaa
      181 gtttattatc tattggaagt taaataaata tgtaaggaat taaaatgaaa aaaagtacag
      241 tattgtcatt aaccacagct gcagttattt tagcagccta tgcccctaat gaggtagtct
      301 tagcagacac atctagctct gaagatgctt taaacatctc tgataaagaa aaagtagtat
      361 tagataagga aacagaaaat aaagagaaac atgaagatat tcataatgct atagaaactt
      421 cacaggattt taaagagaag aaaacagcag tcattgaggg aaaagaagtt gttagtaaaa
      481 atcctgtgac agacaataac actagcaatg aagaagcaag aatcaaagaa gattccaatc
      541 aatcccaagg agatcatacg gactcatttg tgaataaaaa cacagaaaat cccaaaaaag
      601 aagataaagt tgtctatatt gctgaattta aagataaaga atctggagaa aaagcaatca
      661 agcaactatc caatcttaag aatacaaaag ttttatatac ttatgataga atttttaacg
      721 gtagtgccat agaaacaact ccagataact tggacaaaat taaacaaata gaaggtattt
      781 catcggttga aagggcacaa aaagtccaac ccatgatgaa tcatgccaga aaggaaattg
      841 gagttgagga agctattgat tacctaaagt ctatcaatgc tccgtttggg aaaaattttg
      901 atggtagagg tatggtcatt tcaaatatcg atactggaac agattataga cataaggcta
      961 tgagaatcga tgatgatgcc aaagcctcaa tgagatttaa aaaagaagac ttaaaaggaa
     1021 ctgataaaaa ttattggttg agtgataaaa tccctcatgc gttcaattat tataatggtg
     1081 gcaaaatcac tgtagaaaaa tatgatgatg gaagggatta ttttgaccca catgggatgc
     1141 atattgcagg gattcttgct ggaaatgata ctgaacaaga tatcaaaaac tttaacggca
     1201 tagatggaat tgcacctaat gcacaaattt tctcttacaa aatgtattct gacgcaggat
     1261 ctgggtttgc gggtgatgaa acaatgtttc atgctattga agattctatc aaacacaacg
     1321 ttgatgttgt ttcggtatca tctggtttta caggaacagg tcttgtaggt gagaaatatt
     1381 ggcaagctat tcgggcatta agaaaagcag gcattccaat ggttgtcgct acgggtaact
     1441 atgcgacttc tgcttcaagt tcttcatggg atttagtagc aaataatcat ctgaaaatga
     1501 ccgacactgg aaatgtaaca cgaactgcag cacatgaaga tgcgatagcg gtcgcttctg
     1561 ctaaaaatca aacagttgag tttgataaag ttaacatagg tggagaaagt tttaaataca
     1621 gaaatatagg ggcctttttc gataagaata aaatcacaac aaatgaagat ggaacaaaag
     1681 ctcctagtaa attaaaattt gtatatatag gcaaggggca agaccaagat ttgataggtt
     1741 tggatcttag gggcaaaatt gcagtaatgg atagaattta tacaaaggat ttaaaaaatg
     1801 cttttaaaaa agctatggat aagggtgcac gcgccattat ggttgtaaat actgtaaatt
     1861 actacaatag agataattgg acagagcttc cagctatggg atatgaagcg gatgaaggta
     1921 ctaaaagtca agtgttttca atttcaggag atgatggtgt aaagctatgg aacatgatta
     1981 atcctaataa aaaaactgaa gtcaaaagaa ataataaaga agattttaaa gataaattgg
     2041 agcaatacta tccaattgat atggaaagtt ttaattccaa caaaccgaat gtaggtgacg
     2101 aaaaagagat tgactttaag tttgcacctg acacagacaa agaactctat aaagaagata
     2161 tcatcgttcc agcaggatct acatcttggg ggccaagaat agatttactt ttaaaacccg
     2221 atgtttcagc acctggtaaa aatattaaat ccacgcttaa tgttattaat ggcaaatcaa
     2281 cttatggcta tatgtcagga actagtatgg cgactccaat cgtggcagct tctactgttt
     2341 tgattagacc gaaattaaag gaaatgcttg aaagacctgt attgaaaaat cttaagggag
     2401 atgacaaaat agatcttaca agtcttacaa aaattgccct acaaaatact gcgcgaccta
     2461 tgatggatgc aacttcttgg aaagaaaaaa gtcaatactt tgcatcacct agacaacagg
     2521 gagcaggcct aattaatgtg gccaatgctt tgagaaatga agttgtagca actttcaaaa
     2581 acacggattc taaaggtttg gtaaactcat atggttccat ttctcttaaa gaaataaaag
     2641 gtgataaaaa atactttaca atcaagcttc acaatacatc aaacagacct ttgactttta
     2701 aagtttcagc atcagcgata actacagatt ctctaactga cagattaaaa cttgatgaaa
     2761 catataaaga tgaaaaatct ccatatggta agcaaattgt tccagaaatt cacccagaaa
     2821 aagtcaaagg agcaaatatc acatttgagc atgatacttt cactataggc gcaaattcta
     2881 gctttgattt gaatgcggtt ataaatgttg gagaggccaa aaacaaaaat aaatttgtag
     2941 aatcatttat tcattttgag tcagtggaag aaatggaagc tctaaactcc aacgggaaga
     3001 aaataaactt ccaaccttct ttgtcgatgc ctctaatggg atttgctggg aattggaacc
     3061 acgaaccaat ccttgataaa tgggcttggg aagaagggtc aagatcaaaa acactgggag
     3121 gttatgatga tgatggtaaa ccgaaaattc caggaacctt aaataaggga attggtggag
     3181 aacatggtat agataaattt aatccagcag gagttataca aaatagaaaa gataaaaata
     3241 caacatccct ggatcaaaat ccagaattat ttgctttcaa taaccaaggg atcaacgctc
     3301 catcatcaag tggttctaag attgctaaca tttatccttt agattcaaat ggaaatcctc
     3361 aagatgctca acttgaaaga ggattaacac cttctccact tgtattaaga agtgcagaag
     3421 aaggattgat ttcaatagta aatacaaata aagagggaga aaatcaaaga gacttaaaag
     3481 tcatttcgag agaacacttt attagaggaa ttttaaattc taaaagcaat gatgcaaagg
     3541 gaatcaaatc atctaaacta aaagtttggg gtgacttgaa gtgggatgga ctcatctata
     3601 atcctagagg tagagaagaa aatgcaccag aaagtaagga taatcaagat cctgctacta
     3661 agataagagg tcaatttgaa ccgattgcgg aaggtcaata tttctataaa tttaaatata
     3721 gattaactaa agattaccca tggcaggttt cctatattcc tgtaaaaatt gataacaccg
     3781 cccctaagat tgtttcggtt gatttttcaa atcctgaaaa aattaagttg attacaaagg
     3841 atacttatca taaggtaaaa gatcagtata agaatgaaac gctatttgcg agagatcaaa
     3901 aagaacatcc tgaaaaattt gacgagattg cgaacgaagt ttggtatgct ggcgccgctc
     3961 ttgttaatga agatggagag gttgaaaaaa atcttgaagt aacttacgca ggtgagggtc
     4021 aaggaagaaa tagaaaactt gataaagacg gaaataccat ttatgaaatt aaaggtgcgg
     4081 gagatttaag gggaaaaatc attgaagtca ttgcattaga tggttctagc aatttcacaa
     4141 agattcatag aattaaattt gctaatcatg ctgatgaaaa ggggatgatt tcttattacc
     4201 tagtagatcc tgacaaagat gcttctaaat atgaaaaact tggtgaaatt tctgaagata
     4261 aactcaaaaa tgcaaagagc ccagaggaaa ataccaataa taatcaagct aaggatgaag
     4321 attcaaaacc agatgaaaaa agttcagttg agggagaagc tagccttgaa ataaataaaa
     4381 ctatttcaac aattagagag tttgaaaata aagacctaaa gaaactcatt aaaaagaaat
     4441 ttagagaagt tgatgatttt acaagtgaaa ctggtaagag aatagaggaa tacgattata
     4501 aatacgatga taagggaaat attattgctt atgacgatgg tagtgcctta caatatgaaa
     4561 ctgaaaaact tgacgaaata aaatcaaaaa tttatggtgt tctaagccca tctaaagatg
     4621 gacactttga aattcttgga aagataagca atgtttctaa aaatgccaag gtatattatg
     4681 gaaatagcta taaatcgata gaaatcaaag cgactaagta tgattcacat tcaaaaacga
     4741 tgatatttga tttatacgct aatattaatg atattgtaga tggattagct tttgctggag
     4801 atatgagatt atttgttaaa gatgacaatc aaataaaagc tgaaactaag attagaatgc
     4861 ctgaaaaaaa taaggaaact aaagcagaat atccctatgt atcaagttat gggaatgtaa
     4921 tcgaattagg agaaggagat ctttcaaaga acaaaccaga caatttaact aaaatggaat
     4981 ctggtaaaat ctattctgat tcagaaaaac aacaatatct gttaaaggat aacatcattc
     5041 taagaaaagg ctatgcacta aaagtgacta cctataatcc tggaaaaacg gatatgttag
     5101 aaggaaatgg agtctatagc aaggaagata tagcaaaaat acaaaaggcc aatcctaatc
     5161 taagagtcct ttcagaaaca acaatttatg ctgatagtag aaatgttgaa gatggaagaa
     5221 gtacccaagc tgtattaatg tcggctttgg acggctttaa cattataagg tatcaagtgt
     5281 ttacatttaa aatgaacgat aaaggggaag ctatcgataa agacggaaat cttgtgacag
     5341 attcttctaa acttgtatta tttggtaagg atgataaaga atacactgga gaggataagt
     5401 ccaatgtaga agctataaaa gaagatggct ccatgttatt tattgatacc aaaccagtaa
     5461 acctttcaat ggataagaac tactttaatc catctaaatc taataaaatt tatgtacgaa
     5521 atccagaatt ttatttaaga ggtaagattt ctgataaggg tggttttaac tgggaattga
     5581 gagttaatga atcggttgta gataattatt taatctacgg agatttacac attgataaca
     5641 ctagagattt taatattaag ctgaatgtta aagacggtga catcatggac tggggaatga
     5701 aagactataa agcaaacgga tttccagata aggtaacaga tatggatgga aatgtttatc
     5761 ttcaaactgg ctatagcgat ttgaatgcta aagcagttgg agtccactat cagtttttat
     5821 atgataatgt taaacccgaa gtaaacattg atcctaaggg aaatactagt atcgaatatg
     5881 ctgatggaaa atctgtagtc tttaacatca atgataaaag aaataatgga ttcgatggtg
     5941 agattcaaga acaacatatt tatgtaaatg gaaaagaata tacatcattt gatgatatta
     6001 aacaaataac agacaagaca ctaaacatta agattgttgt aaaagatttt gcaagaaata
     6061 caaccgtaaa agaattcatt ttaaataaag atacgggaga ggtaagtgaa ttaaaacctc
     6121 atagggtaac tgtgaccatt caaaatggaa aagaaatgag ttcaacgata gtgtcggaag
     6181 aagattttat tttacctgtt tataagggtg aattagaaaa aggataccaa tttgatggtt
     6241 gggaaatttc tggtttcgaa ggtaaaaaag acgctggcta tgttattaat ctatcaaaag
     6301 atacctttat aaaacctgta ttcaagaaaa tagaggagaa aaaggaggaa gaaaataaac
     6361 ctacttttga tgtatcgaaa aagaaagata acccacaagt aaaccatagt caattaaatg
     6421 aaagtcacag aaaagaggat ttacaaagag aagatcattc acaaaaatct gattcaacta
     6481 aggatgttac agctacagtt cttgataaaa acaatatcag tagtaaatca actactaaca
     6541 atcctaataa gttgccaaaa actggaacag caagcggagc ccagacacta ttagctgccg
     6601 gaataatgtt tatagtagga atttttcttg gattgaagaa aaaaaatcaa gattaagaca
     6661 aaagctatag aaaaaaatgg tttatgtact gagattagat agtgaggtga tgacatagtt
     6721 ttgtgaaaac agccatttat aactcaatta tttagtttac ttcactattg atactatttg
     6781 gagttattaa tggacttagt ttagataact aatgaattga ttgaaagggt tagtattgac
     6841 aatattggtc atattgatta gaaaatagag tctatcaaaa tttaaaggct aatagaggtg
     6901 atgagacaat ttcggctctt tgtcaactgt agtgggttga agtcagctaa gctcgagaaa
     6961 ggacaaattt tgtcctttct tttttgat
//
LOCUS       DAVBCG010000097         6890 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBCG010000097 DAVBCG010000000
VERSION     DAVBCG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.097"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            71..1348
                     /locus_tag="VQ495_001604"
     CDS             71..1348
                     /locus_tag="VQ495_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531540.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            1361..2008
                     /gene="vex2"
                     /locus_tag="VQ495_001605"
     CDS             1361..2008
                     /gene="vex2"
                     /locus_tag="VQ495_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2531541.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            2060..3439
                     /gene="vex3"
                     /locus_tag="VQ495_001606"
     CDS             2060..3439
                     /gene="vex3"
                     /locus_tag="VQ495_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2531542.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            3619..3702
                     /gene="pep27"
                     /locus_tag="VQ495_001607"
     CDS             3619..3702
                     /gene="pep27"
                     /locus_tag="VQ495_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2531543.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            3751..4407
                     /gene="vncR"
                     /locus_tag="VQ495_001608"
     CDS             3751..4407
                     /gene="vncR"
                     /locus_tag="VQ495_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2531544.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            4404..5732
                     /gene="vncS"
                     /locus_tag="VQ495_001609"
     CDS             4404..5732
                     /gene="vncS"
                     /locus_tag="VQ495_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2531545.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            5873..6754
                     /locus_tag="VQ495_001610"
     CDS             5873..6754
                     /locus_tag="VQ495_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2531546.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
BASE COUNT         2112 a         1346 c         1515 g         1917 t
ORIGIN      
        1 ttttttttcg tatttcataa acatttcata tttgggtttt ataatagtct tacaaatatg
       61 gaggtgacaa atgaatccaa tccaaagatc ttgggcttat gtcagcagaa agcgactgag
      121 aagttttatt ttatttctga ttttattggt cttattggcc ggaatttcag cctgtttgac
      181 tctgatgaag tccaacaaaa cagtagaaag caatctttat aaatcactca atacatcttt
      241 ttctattaag aagatagaga atggtcagac attcaagttg tcagacctag catctgtaag
      301 caagattaag gggctggaaa atgtctctcc tgaacttgag acggtcgcaa aactaaaaga
      361 caaggaagca gtgactggtg agcagagcgt ggagcgtgat gatttatcag ctgcagacaa
      421 taacttggtt agcttaacgg ctcttgagga ttcatccaag gatgtaacct ttaccagttc
      481 ggctttcaat ctaaaagaag ggcgacacct tcaaaaaggg gattccaaga aaatccttat
      541 ccacgaagaa ttggctaaga agaacggtct ttcacttcat gacaagattg gcttggatgc
      601 tggtcagtct gaatctggaa aaggacaaac agtagagttt gagattatcg gcatcttttc
      661 tggtaaaaaa caagagaaat tcacaggctt gtcttctgac ttcagtgaaa atcaagtctt
      721 tacagactat gaaagtagcc aaaccctttt gggcaatagt gaagctcaag tcagtgcagc
      781 acgcttctat gtagaaaatc ctaaggaaat ggacggactc atgaagcagg tagaaaactt
      841 ggccttggaa aatcaaggct accaagtcga aaaggaaaac aaggcttttg aacaaatcaa
      901 agactcagtt gcaactttcc aaaccttctt gaccatcttc ctttatggga tgttgatagc
      961 aggagctgga gccttaattc tggttttgtc tctctggttg agagaacggg tctatgaagt
     1021 ggggatttta cttgcacttg gaaaaggcaa gagctcgatc ttcctacaat tctgtttaga
     1081 ggtagttttg gtatctcttg gagctttgct tccagcattt gttgcaggaa acggaatcac
     1141 aagttatcta ctccaaactc tactagcaag tggagatcag gcaagcttac aagatacact
     1201 agccaaagca agcagtttat caactagcat cttatctttt gcagaatcct atgtttttct
     1261 agttctgctt agttgcttat ctgtagccct ttgtttccta ttcttattta gaaaatcacc
     1321 gaaagaaatt ttatcatcta ttagttaaga aggagaaatc atgactttat tacaattaca
     1381 agatgttacc taccgttata agaatactgc tgaagcagtc ctatatcaga tcgattataa
     1441 ttttgaacct ggaaaatttt acagtattat tggggagtca ggagcaggaa aatccacact
     1501 cttgtcccta cttgctggtc tagatagtcc tgttgaaggt tctatccttt ttcaaggaga
     1561 ggatattcgt aagaagggct attcttacca tcgcatgcac catatttccc tggtctttca
     1621 aaattataac ttgatagatt atctttctcc gctggaaaat atccgcttgg tcaacaaaaa
     1681 ggcaagcaag gatacacttc ttgagcttgg tttggatgaa agccagatca agcggaatgt
     1741 tctccagtta tcaggtggtc aacagcaacg tgttgccatt gctcgcagtt tggtctcaga
     1801 agctccagtt attctagctg atgagccaac aggaaatctg gatcctaaaa ctgctggaga
     1861 tattgtcgaa ctactcaaat cacttgccca gaaaacaggt aaatgtgtga ttgtcgtaac
     1921 tcacagtaaa gaagtggcac aagcgtcaga tattacactt gaattaaagg ataagaaact
     1981 gactgaaacg cgcaatacta gtaaataatt tgagcttatt ttgatagaat gattaaaaca
     2041 aaatctagaa agggaatcta tgttacacaa cgcatttgcc tatgttacaa ggaagttttt
     2101 caaatcgatt gtcatcttcc tgattattct cctcatggcg agcttgagtt tggtcggctt
     2161 gtcaatcaag ggagctactg ccaaggcttc tcaggagacc tttaaaaata tcactaatag
     2221 cttctccatg caaatcaatc gtcgcgtcaa ccaaggaacg cctcgtggtg ctgggaatat
     2281 caagggtgaa gacatcaaaa aaatcaccga aaacaaggcc atcgagtctt atgtcaaacg
     2341 tatcaacgct atcggagatt tgactggata tgacctgatt gaaacgccag aaaccaagaa
     2401 gaatctcact gctgatcgtg ccaagcgttt tggaagtagc ttgatgatta caggtgtcaa
     2461 tgactcctct aaagaagaca agtttgtctc tggttcttat aaactagtcg aaggagagca
     2521 cttaaccaac gacgacaagg ataaaatcct cttgcacaag gacttggcag ccaaacacgg
     2581 ctggaaagta ggggacaagg ttaaactgga ctctaatatc tacgatgcag ataatgaaaa
     2641 aggagccaag gaaacagttg aagtgacaat caagggactc tttgatggtc ataataagtc
     2701 agcagtaacc tactcacaag aactttacga aaacacagct attacagaca ttcacactgc
     2761 tgcaaaactt tatggataca cagaagacac agccatttat ggggacgcaa ccttctttgt
     2821 aacagcagac aagaacttgg atgatgttat gaaagagttg aatggcatca gtggtatcaa
     2881 ctggaagagc tacacactcg tcaagagctc ctctaactac ccagctcttg agcaatctat
     2941 ctctggtatg tacaagatgg ccaacctcct cttctggggt agcttgagct tctcagttct
     3001 cctccttgcc ctcttgctca gcctttggat caacgcccgt cgcaaggaag tgggaattct
     3061 cctctctatc ggcctcaagc aggcaagtat cttgggtcaa ttcatcaccg aatctatctt
     3121 gattgctatc cctgctctag tttctgctta cttcctagct aattacactg cccgtgcaat
     3181 tggaaacact gtccttgcca atgtgacttc aggtgttgcc aaacaggcta gtaaggcggc
     3241 tcaagcctct aaccttggtg gtggtgcaga agtagatggc tttagcaaga ccttgtcgag
     3301 cctagacatt tccattcaga catcagactt tatcatcatt tttgtccttg ccttggttct
     3361 agtggttctc gttatggcgc ttgcttcaag caatctcctt agaaaacaac caaaagagct
     3421 cttgctggat ggtgaataaa tttgaaaaaa tgagtctaga ataaagattg catcttgtgt
     3481 ttctattcaa gaatagtgga taggaatggc tatttaacaa ttcaaaataa atccgaaagc
     3541 agtggtgaaa atcattgctt tcagttgctt tctttgtact ttagtgctta aatataatat
     3601 actaaagtta tggaatttat gagaaaggaa tttcacaacg ttttatctag tggtcagttg
     3661 cttgcagaca aaaggccagc aagagactat aatagaaaat agagtaggta tttattctaa
     3721 gaaaaataaa aaatagagag cagttaaagt atgaaaattt taattgtaga agatgaagag
     3781 atgatccgtg agggggtcag tgattatttg acggattgtg gctatgaaac tattgaggca
     3841 gcggacggtc aggaagctct ggagcaattt tctagctatg aggtggccct ggttttactg
     3901 gatatccaga tgcccaagct caacggctta gaagtcctag ctgagattcg taaaaccagt
     3961 caggttcctg tcttgatgtt gacagctttt caagatgagg aatacaagat gagtgccttt
     4021 gcctctttgg cagatggcta tctggaaaaa cctttctccc tctccctttt aaaagtgagg
     4081 gtggacgcga ttttcaagcg ctactacgat acaggacgaa tcttttctta caaggatacc
     4141 aaggtggact ttgaaagcta cagtgcaagc atcgcaggtc aagaagtgcc tatcaatgcc
     4201 aaagagttgg aaattctgga ctatctagtg aaaaatgaag gccgggcctt gactcgatct
     4261 cagattatcg atgccgtctg gaaagcgaca gatgaggttc cctttgaccg tgttattgat
     4321 gtttatatca aggaattgcg gaaaaagcta gacttggatt gtatcctcac tgtgcgcaat
     4381 gttggttata aattggagcg aaaatgaaac gaacaggttt atttgcaaag atatttatct
     4441 ataccttctc gatatttagt gttctggtta tctgccttca tttagctatt tattttcttt
     4501 ttccttcgac ttatctgagt catcgtcagg aaaccattgg tcaaaaggca acagccattg
     4561 cccagtccct agaagggaaa gataggcaga gtatcgagca agtgttagac ttgtattccc
     4621 agactagtga tatcaagggg accgtcaaag gtgagatgac cgaggacaag ttagaagtca
     4681 aggacagtct tcctctggac acagaccgcc agacaacctc tctctttatt gaggagcgcg
     4741 aggtgaaaac gcaagacggt gctactatga ttctccagtt tctagcttcc atggatttac
     4801 aaaaggaagc ggagcaaatc agtctccagt ttcttcccta taccttgctg gcctcctttc
     4861 tgatttccct cttggtggcc tacatctacg ctcggactat tgttgcaccg atcttggaaa
     4921 tcaagcgggt gacccgtcgg atgatggacc tggattccca agtgcgattg cgcgtggatt
     4981 ctaaggatga gataggtaat ctcaaggaac aaatcaatag cctctaccag catctcttga
     5041 ctgttattgc ggacttgcat gaaaagaatg aagccattct ccagctggag aagatgaagg
     5101 tcgaattcct acgaggagct tctcatgaat tgaaaacacc gctggctagt ttgaaaatcc
     5161 taatcgaaaa tatgagagag aatatcggtc gttataagga tagagaccag tatctgggag
     5221 ttgccttggg gattgtggat gaactcaatc accatgttct gcagatactt tccctctctt
     5281 ctgtgcagga attgcgagat gatagggaaa caattgacct cctccagatg acgcaaaatc
     5341 tggtcaaaga ttatgccttg ctagccaagg aaagagagct ccagatagac aatagtttga
     5401 cccatcagca ggcttatcta aacccatcag ttatgaagtt gattctttct aatctcatca
     5461 gcaatgccat taagcactct gttccaggtg gcttagttcg aattggagaa agagaaggag
     5521 aactttttat cgaaaatagc tgtagctcag aggaacaaga aaaactagcc cagtcttttt
     5581 ctgacaatgc cagtcgcaag gtcaaggggt ctggtatggg gctctttgtg gttaagagtc
     5641 tattagaaca tgaaaaatta gcttatcgtt tcgagatgga ggagaatcgt ttaaccttct
     5701 ttatagattt tccaaaagtc gtccaagact agggagagaa agggtttaca tagatggagt
     5761 tagaagaaaa tcaatcgaaa ctgcgggaaa aactagattt ttttggcaaa aagtgataaa
     5821 atgaacaatg taaatgggat gacccataaa aatatacagg aggcctgata aaatggcaat
     5881 cgtttcagca gaaaaatttg tccaagcagc ccgtgacaac ggttatgcag ttggtggatt
     5941 taacacaaac aaccttgagt ggactcaagc tatcttgcgc gcagcagaag ctaaaaaagc
     6001 tccagttttg atccaaactt caatgggtgc tgctaaatac atgggtggtt acaaagttgc
     6061 tcgcaacttg atcgctaacc ttgttgaatc aatgggtatc actgtaccag tagctatcca
     6121 ccttgaccac ggtcactacg aagatgcact tgagtgtatc gaagttggtt atacttcaat
     6181 catgtttgac ggttcacacc ttccagttga agaaaacctt aaattggcta aagaagttgt
     6241 tgaaaaagca cacgctaaag gtatctcagt agaagctgaa gttggtacta tcggtggtga
     6301 agaagacgga atcatcggta aaggtgaatt ggctccaatc gaagacgcta aagcaatggt
     6361 tgaaactggt atcgacttct tggcagctgg tatcggtaac atccacggcc cttacccagt
     6421 aaactgggaa ggtcttgacc ttgaccactt gcaaaaattg acagaagctc ttccaggatt
     6481 cccaatcgta ttgcacggtg gatcaggtat tcctgatgag caaatccaag cagctatcaa
     6541 acttggtgtt gccaaagtta acgttaacac agaatgccaa atcgcattcg ctaacgcaac
     6601 tcgtaaattt gctcgtgact acgaagcaaa cgaagcagaa tacgacaaga aaaaactctt
     6661 cgacccacgt aaattcttgg ctgacggtgt aaaagctatc caagcatcgg ttgaagaacg
     6721 tatcgacgta ttcggttcag aaggtaaagc ataatctagc tgaataatac agataaaacc
     6781 tgcctgtgag ggtgggtttt ttgatttcaa gctactcgtt ttagtatagt aaactgaatc
     6841 tagactagca caatgctgct tctatagcgt tttgaaacta gaataggaca
//
LOCUS       DAVBCG010000098         6795 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBCG010000098 DAVBCG010000000
VERSION     DAVBCG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.098"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..207)
                     /locus_tag="VQ495_001611"
     CDS             complement(<1..207)
                     /locus_tag="VQ495_001611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531547.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFF"
     gene            complement(275..916)
                     /locus_tag="VQ495_001612"
     CDS             complement(275..916)
                     /locus_tag="VQ495_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531548.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(918..2948)
                     /locus_tag="VQ495_001613"
     CDS             complement(918..2948)
                     /locus_tag="VQ495_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2531549.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(3217..4116)
                     /locus_tag="VQ495_001614"
     CDS             complement(3217..4116)
                     /locus_tag="VQ495_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531550.1"
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ495_001615"
     CDS             complement(4188..4748)
                     /gene="rnmV"
                     /locus_tag="VQ495_001615"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2531551.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(4748..5521)
                     /locus_tag="VQ495_001616"
     CDS             complement(4748..5521)
                     /locus_tag="VQ495_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2531552.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(5826..6719)
                     /locus_tag="VQ495_001617"
     CDS             complement(5826..6719)
                     /locus_tag="VQ495_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2531553.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
BASE COUNT         1908 a         1619 c         1172 g         2096 t
ORIGIN      
        1 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
       61 cacatcatcc actgtcagtt caaccgtttg aggtaaattt ttcaaacgat aatgcttttg
      121 cctttgccct tcactactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
      181 aaggagagga ttggtcatat tttccatgat gaggatatct tataaaccac ctctggcact
      241 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
      301 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
      361 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
      421 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gttttcgcaa
      481 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
      541 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
      601 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
      661 caaaattttg aaagagatag cccatctgat ctcgaaaata ctcacgagtg ggaatggttt
      721 ttaaatcttt cccctgatag agaacccttc ctccatctat cttttctagc tttcccaaga
      781 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
      841 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
      901 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
      961 ttgtcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
     1021 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
     1081 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
     1141 atcataagct cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
     1201 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc tacccgtttg
     1261 tcatttagta ccttggcaaa aaatagctgg ctttttacca agtaagaaac cttgtgatac
     1321 agaccttgct cttttagagc tgtgatgctg tctccatatc gatctagttt caaggcacta
     1381 ttagccgtag ttccccacaa catccctgca acaggtcttg ttccagtcgc ttgcggcgtt
     1441 agaactacaa taatcggatc ttttaggtac tggcgtgttg tcttgtaccc atcattatag
     1501 aggaaacgtt cctgtcctgt ttctgtaaaa gctagactta cctgtgggag gtagtatttc
     1561 tgactcgtca cttctacact ttcagatgaa aagttttgca ggtaatctgt aaacaatctt
     1621 tggtagtagg tagactgctc tcgcaagctc tcaggcaaga ttagcccaaa ctctccctca
     1681 gacaagtttt gcatcttgtc cataaactct gaagaaacgg taatcttttc ttctatcaga
     1741 tagcgtggtg agacatagat aacattccct gacggtgtgt agtcactgag acgattgcca
     1801 tctaggtcca cttctgctcc gtctgagaaa ttgtcaacat tgctcataat ataaaaagag
     1861 tctgtattgg ctaaccgttc ttcagtaaat gtctgccact cacgattatc cttacgcgtt
     1921 ccttcttcat cggcaaatgc actagaccaa ccaaaggata gacggtaacg gtctgaggac
     1981 tggctccatt tattgctagc tctctccatt tcctgcattt cacggtagtg gggtaggaga
     2041 gctgtcgcac tcgatccgac caccaataca gctaagagtt gccccaccat catcaatgtc
     2101 atcatacgtt tgagagggag tttccctttc aatagatcca ccagactatt ttcctgtaaa
     2161 ccaagtagat agacgacact tagtaaggta gaaatccctg ccagggtcaa tccataaagt
     2221 agaagagcaa tgatgaccag ttgcaccgtt gccataaaca aggcaccttg ataccagaga
     2281 atccccaatc ccaaaagact ggataccagc actgagcaga taaactgtct cacatcttct
     2341 aacactggtc tgagagcaac tccaaacaag ctctcaccag ctattaagcg aatccctgcc
     2401 tgacgaaggg atttgatccg ataaatcagg gtcagactca taaaggtcag cagaaaaata
     2461 gctaaactca gtagcaccat aggggtggcc gccaatagta agactatcga aaatggatct
     2521 tctccattcg aaacaaagcc ttgatagcca agttctttca aggtggtctg taagctcact
     2581 ccatccaaac ttcctgacac aatcaagtaa gaccctaata aatcactcgt ttctgcactc
     2641 tccttggagg aaatggtcag accttctgga agctctccct caccataagt ggcataggta
     2701 aagagtgttt ctccagcttc atttggctca acgattcgac gggcgatgag actattatgc
     2761 tctaccgcca agtgactcaa aacctctgtc acctcttcgc gactagctac tggttgattt
     2821 atcatcattc ctgtcacatc aacagaagga tagttaccaa ataaaatctc ttctcgatgg
     2881 tcaacggaag ttatcaccat ataaagcgat accaatacca ttgaaatcaa aataaataaa
     2941 cgtttcatca tttcctccaa aaagttactt ggtcacttag tgagaaggtg accacagtca
     3001 ctgcgatgtt aagaagctta ttaagttctt tctactagca acatcttcaa atacattata
     3061 tccgtttttt caagaactat caaggacatc aatgtcctta aaatctaact atttcatatt
     3121 atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact gttttgatac
     3181 gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt tgacatcctc
     3241 ttgccattcc tctgtcaatt tctttactag aaatccatct ccaattagct tcgcaaatgt
     3301 tttagaagac tgataaaatg attcagctaa actaaaatct ctttcttctc ttaatgcttg
     3361 cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt tatccataat
     3421 ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct tttcaagaca
     3481 acacattcct gaaataagag tatctctaat tataaagaag ttttctaatt tagttacctt
     3541 tttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt tggcaagatc
     3601 ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat ttgttgaata
     3661 aacttctttt tctatgatct gagacatatg ttcctcaagt attaactctg catattcagg
     3721 ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta attgttcttc
     3781 tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt ccttcttatc
     3841 gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca attctaaata
     3901 agcaggagga agagctgaaa aatccggcat aaggctataa actggaactc ctagccggcg
     3961 agcaatatag gctaacttgg gcaaactcgg ttgcgaagct ccactttcaa ttcgcgacag
     4021 ttgacgaact gtcagttctt gctcatctcc acaaaaatat ggacgactaa ctcctttttc
     4081 tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct tctacctgct
     4141 tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta ctcataagat
     4201 ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg tttgaggagt
     4261 tgcttgccgt tagaatagcc gattcggaga gtctctccga gatattctct acgcttacgg
     4321 ctgtctgccc ctgctagaaa accaaggcga atcaagtcgc tacgactaat gtcaaactga
     4381 ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc ttcatagctg
     4441 gcatgctcaa ttcccagaga acgccccttg gtcttggact ttggaacagc ttcatctcgc
     4501 ttgagaaagg catgccgaac tgttggaatg gccgtcataa tcatgcgccg aatccgctcc
     4561 ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgcag gcgctgaatc
     4621 cgctctatat cctgagcatt gatggcagaa cctcgagtct cataggtctc cacatcgaaa
     4681 taacgtttga gattgaccgt atcatcacgc ccttcaacca cgataacttg agaaattttc
     4741 tctttcatta cttgctgtcc aatccaaaaa tgcgttctgc atttgcagtc gttgctaccg
     4801 ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca tagcgagtat
     4861 aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc gcatctgttt
     4921 ccaccaaaat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca gttgccttct
     4981 taaaagtcac cactcctgag aaggaaatgg tcataccaag atccacaaac ttctctgccc
     5041 actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg ccctcactct
     5101 tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa ggcaaatcca
     5161 agtccttaga tagctgaatc tgacggcgaa aaacctgctc ctgcacctct ttgggcgctg
     5221 tcatccaatg gtaatctaag ccaatttcac ctaaagccac aaccttggga tgttttaact
     5281 tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta ggatgccaac
     5341 caatagtcgc atagagctga tcatactcat ctaccaactc caaggcacgc tcaatcgtcg
     5401 gtttatcaaa accaacaata ttcatctgtg tcacacccat ctcagcagcc aaggcaattt
     5461 cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta tcaaaaatca
     5521 tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg gcacctaatt
     5581 tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc tatccgatat
     5641 tgcccatctc ctattccaca gttagagaca gaagagattg gctacattga tagaatattc
     5701 ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca tcactaggat
     5761 tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag aattttctag
     5821 ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact tgttgccacc
     5881 actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg taattgtttg
     5941 ccagcaggtg actggtaggt aacatcttgc attgttcctg cagttgcttg cggtgcactt
     6001 gttgtcttag gagcagcatt aggagccact ggtctcttag gaactgtaac catgacaatg
     6061 gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc attttcaatc
     6121 tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc tttttcttta
     6181 tcagaaagcg gtgcgccttt gatttcatcc tgcttgtcct tggctgcttt ttcaatggca
     6241 tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg agctgagttt
     6301 tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgcctgcatt cgcattagca
     6361 tctgagcttc cagtattgcc tcccttatct ccacctggtg tattttctag cttatactct
     6421 ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt atcttttcct
     6481 agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc tccagctaca
     6541 ttgattgtcg ctccgtctaa tgctgaacca tttgcttgta aaatagcaac cttaactttt
     6601 tcttcatctg ttaaatgagt tatatctttt acatctactc tttgagcagg tgctttaaca
     6661 gttgattttt cagcagtcgg tttagtgcta tcttgtgctt ttggatcttg tgctaccaat
     6721 tgatttcctg gaattgtaat agctgaacca tctttaaaga cgattgtcgc tgcgccatct
     6781 ccagctactt caact
//
LOCUS       DAVBCG010000099         6777 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBCG010000099 DAVBCG010000000
VERSION     DAVBCG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.099"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            42..743
                     /locus_tag="VQ495_001618"
     CDS             42..743
                     /locus_tag="VQ495_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531554.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            745..3445
                     /locus_tag="VQ495_001619"
                     /pseudo
     CDS             745..3445
                     /locus_tag="VQ495_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(3541..4032)
                     /gene="tpx"
                     /locus_tag="VQ495_001620"
     CDS             complement(3541..4032)
                     /gene="tpx"
                     /locus_tag="VQ495_001620"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2531555.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(4155..5084)
                     /gene="psaA"
                     /locus_tag="VQ495_001621"
     CDS             complement(4155..5084)
                     /gene="psaA"
                     /locus_tag="VQ495_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2531556.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(5116..5964)
                     /locus_tag="VQ495_001622"
     CDS             complement(5116..5964)
                     /locus_tag="VQ495_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2531557.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(5961..6683)
                     /locus_tag="VQ495_001623"
     CDS             complement(5961..6683)
                     /locus_tag="VQ495_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2531558.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         1951 a         1328 c         1523 g         1975 t
ORIGIN      
        1 attttcgagg agtataattt gtttgataga gggtgggtct gatggcttat attgagatga
       61 aacactgtta caagcgttat caggttgggg acacggagat tgtggccaat cgtgatgtga
      121 attttgagat tgaaaagggg gagctggtta ttatccttgg tgcttcaggt gcaggcaagt
      181 caacagttct taaccttctt gggggaatgg ataccaatga tgaaggggaa atctggattg
      241 atggtgttaa tattgcggat tatagttccc accagcgtac caattaccgt agaaatgatg
      301 tggggtttgt ttttcagttt tataatctag tttctaatct gacagctaag gaaaatgtgg
      361 aactggcttc tgaaattgtg acagatgcct tgaatcctga tcaggtcttg acagatgtag
      421 gtctggctca tcgtctcaat aactttccag cccagctttc tggaggggag caacagcgag
      481 tctccattgc acgcgcggta gccaaaaatc ctaaaattct cctttgtgat gaaccgactg
      541 gagccttgga ttatcagacg ggcaagcagg ttttgaaaat tctccaagac atgtctcgtc
      601 aaaagggagc gacggtgatc atcgtgactc ataatggagc tttggcgccc attgctgatc
      661 gcgtgattca aatgcacgat gccagtgtca aggatgtggt gctcaaccag catcctcagg
      721 atattgacag tttggagtac tagcatgatc aagcgaaaaa cttattggaa ggacttaatt
      781 cagtccttca caggctccaa ggggcgtttt ttatccatct tgatcctgat gatgttggga
      841 tctctagcct tagtaggcct caaagtaacc agtcccaaca tggaggcgac agctaatgct
      901 tatttaacaa ctgctcaaac cttggatttg gcagtcatgt ctaactatgg cttggatcaa
      961 gcagaccaag aagaactaaa acagacggag ggcgcagagg tcgagtttgg ctatttgaca
     1021 gatgtgacta tggataatgg gcaggatgcc attcggctgt actccaaacc agagcgaatt
     1081 tcaacctttc agctaagaaa gggacgactt cctcagtcag acaaggaaat cgctttggcc
     1141 actcatttgc aaggccaata cagcgtggga caggagatta gttttaaaga aaaagaagag
     1201 ggtcattcct ctttaaaaga ccatacttat accattactg gttttgtgga ttcggctgaa
     1261 atcctctccc agcgagatat gggctacgca ggaagtggaa gtgggactct gacagcctat
     1321 ggggtgattt tacctagtca gtttgatcag aaagtctaca atatagctcg tttgaaatat
     1381 caagatttag caggtttaaa tgccttttca tcagcttatg aagaaaaatc caagcaacat
     1441 caggaagacc ttgaacaaac tttatcagat aatggcaagg tacgtctgca acttttgaaa
     1501 aaagaaggac aagagtctct agacaagggg caagagaccc ttgacaaggc tcagactaat
     1561 ttgcaggaag gcaagcgtcg tttagcagct gctcaagctc gtatacaggc tcaagaaagt
     1621 caactagcct tgtttcctca agttcagaga gagcaggcta gtgctcaact tacccaagcc
     1681 aagcaggaat tgggcaagga agaggataaa ctaaagcaag ctgaacaaaa tctagcccaa
     1741 gaaaaggaaa agttagaaaa acatcagcaa gtcttggacg atttggcgga gccaaggtat
     1801 caggtctata atcgtcagac catgccaggt ggtcagggct atcttatgta tagcaatgct
     1861 tcatccagta ttcgagcagt gggcaatatc tttcctgtgg tactttatgc cgtagcatcc
     1921 atggtgacct ttacgaccat gactcgcttt gtagacgaag agcgaactca tgcagggatt
     1981 tttaaggcct tgggttaccg tagtaaggat attatcacca agtttctcct ttatggacta
     2041 gtagctggga ctgtcggaac ggctctaggt agtatacttg gtcattattt gctagccagt
     2101 gtaatttcaa gtgtcattac aaaaggcatg gtggtgggag aaacccagat tcagttctat
     2161 tggacctata gcttactagc tcttgtcttg agctggttgg cgagtgtgtt accagcctat
     2221 ctggtggctt ggagggaact tcatgccgaa gcagcccagc ttctacttcc taaacctcct
     2281 gtcaaaggag ctaaaatctt attggagcgt atcggtttta tctggcgtcg tctcagtttt
     2341 actcataagg taacagcccg caacatcttt cgttataagc agagaatgtt gatgacaatc
     2401 tttggtgtgg caggttctgt agctctgctc tttgcaggtt tgggaatcca atcttctgta
     2461 gcaggagttc cgtctaaaca gtttcaacaa atccaacagt atcagatgct tgtctctgaa
     2521 aatcctagtg cgaccaatca ggacaaggta gagctagcag aagtgttgaa agggcaggag
     2581 atactagcct accagaaaat ctattctaaa acgctagaca aggatttcaa aggcaaggct
     2641 ggtcttcaaa acattactct tatgatgata gagaaggaag atttgactcc ctttatccat
     2701 cttcaacatc atcagcagga gctgacatta aaagatggca tcgttattac agctaaactc
     2761 gcccagctgg caggtgtcaa ggttgggcag actttagaaa ttgaaggtaa ggaactaaag
     2821 gtcgctgcta ttactgagaa ctacgttggt cactttattt atatgagtca ggctagctat
     2881 gagcaacttt acggacagct accccaagcc aacacttatc tggtctcatt aagggatacc
     2941 agtgcaacta gtatcgaaag tcaggcgggc ttgcttatga atcaatctgc ggtgtccagc
     3001 gttgtccaaa atgcttcagc cattcgactc tatcgctagc tcactcaatc agaccatgac
     3061 catcttggtc atcgtatcag ttctattagc tattgtcatc ctttacaatc tgaccaatat
     3121 caacgtagct gagagaatcc gtgaactctc cactatcaag gttctcggtt ttcataataa
     3181 tgaagtcacc ctctacattt accgtgagac gattgtgctg tcccttgtgg gaatcgtact
     3241 tggtctggta gctggtttct atttacacca atttttgatt caaatgattt cgcctgcgac
     3301 tattctcttt tatccgcagg taggctggga agtctatgta atcccagtgg caacagtaag
     3361 catcattttg accttgcttg gtttcttcgt caattattat ctgagaaagg ttgatatgtt
     3421 agaagccctg aaatctgtag agtaaggtag ttatttttag ctgattgaac ttctatttac
     3481 taatattcaa aaatcctctg tcacaaagag cagggagctc tttgtgacag aggatttttt
     3541 ctatagggct ttagcagctg caattgcggc ttcgaagttt ggctcagaat tgatattatc
     3601 cacgtattca acgtagcgaa tcgtattgtc agtatcgagg acaaagactg cgcgtgctaa
     3661 taggtgccat tcgttgatca agagggcata atcgcgcccg aaagaatggt caaagtagtc
     3721 tgaaagcata atggcattgt caaggccttc agcaccgcac caacgttttt gagcaaaagg
     3781 taggtccatt gaaacagtca atacgaccgt gttgtccagt ccagccaatt cttcattaaa
     3841 acgacgtgtt tgagttgagc agatgcctgt atcgatagaa ggaacgacac tcaagacttt
     3901 tttcttgcca tcaaaatcag ccagagattt tttagaaaga tctgttgtag taagagaaaa
     3961 atcaagcgcc ttgtcgccga cttgtagttg tttacctgta aagctcacag gatttccgag
     4021 aaaagttacc ataggatact ccaatctttt ttcttccatt gtatctgaaa cagtcagaat
     4081 tttccaatga tttgaccgga aatgtgggca tagaaaaaac gccagctcac atgagaatga
     4141 cgtttttcag aggcttattt tgccaatcct tcagcaatct tgtcaaggtt gtatttcatc
     4201 atgctgtagt agctgtcgcc ttctttacct tgttctgcga tagagtcagt aaagatttgt
     4261 gcgtagattg ggatgtttgt gtcttgagaa acagttttca ttggacggtc atccacactt
     4321 gattctacaa agagtgatgg aacttttgtt tggcgaagtt tttcaaccaa ggtcttgatt
     4381 tgttcaggag ttccttcttc ttcagtattg atttcccaga tgtaagcact tgggacacca
     4441 taggctttag agaagtattt gaatgctcct tcgctggtta caatgagttt cttttcagca
     4501 gggatcttat taaatttatc cttactttct ttatcaagtt tgtctaactt atcagtatat
     4561 tctttgagat ttttttcata gaattcttta ttgttagggt ctttggcgct caattgtttg
     4621 gcgatatttt tagcaaaaat aataccgttt tcaaggttaa gccaagcgtg tgggtcttct
     4681 tttccttttt cattttgacc ttcaaggtag ataacatcaa cgccgtcgct gactgcgaag
     4741 tagtctttgt tttcagtttt cttggcattt tctaccaatt ttgtaaacca agcattgcca
     4801 cctgtttcaa ggttgatacc gttatagaaa atcaaatcag cctcagaagt tttcttaacg
     4861 tcttcaggaa gtggttcgta ttcgtgtggg tcttgcccaa tcggaacgat actatgaagg
     4921 tcaattttgt caccagcaat atttttagta atatcagcga tgattgagtt tgtagcaaca
     4981 acttttagtt tttgaccaga agttgtatct ttttttccgc tagcacatgc tacaagaatg
     5041 attgcagaaa gaaagagaac gagtaatgta cctaattttt tcattagatc ctccaaatta
     5101 ttagggcttt gccccttatt ttaacaaatg tttatttttc agtttcaaat atcgttgttt
     5161 gggagcgata aagaagctaa tgagaaagaa actagcagct gtaagcacga tactagaacc
     5221 tgccgcaaca ttaaaactat agccaataaa gagtcccaaa actgaagcag tagctccgaa
     5281 ggttgaggaa aggaaaatca tgcttttcag actattagca tacagataag cagttgcagc
     5341 tggggtaatc agcatggcta caatcaggat agttccgaca ctttgcatgg ctgtcacaga
     5401 cacgagagtc aggagtacca tgagaaggta gtgatagaaa ttgacaggca ttcccatggc
     5461 tttagccaag agttcatcaa aggaagttat caagagttgc ttgaagaaaa tccagattaa
     5521 caagaggatg gctgccccca cacccatagt aataaacata tccgtatctt ggacggccag
     5581 gatattacca aaaaggatat ggaaaaggtc agttgaactt ttagcgacac caatcaagat
     5641 gataccgagg gctaagaaag aagaaaaggt aatgccgatg gcggtatcgc ttttgataat
     5701 cgagtttcct ttgatgtagg taatgatgat ggcagctagc aatccaaaga caatggctcc
     5761 gataaagaag tcaaggccca agatgaagga gagggctaca cctggtaaga cagcatgtga
     5821 aatggcatct cccatgagtg acatcccgcg tagaataatg aaacatccca cagctccagc
     5881 tacaatcccg acgacaatag ctgttatcaa ggcattttgt aggaaatgga atttttgcaa
     5941 tccatcgata aattctgcaa tcataggtca cctccattga aaaagagttg attaccgtaa
     6001 gcttctttta gattggtttc ggtaaaagtt tcttttgttg gaccaaaggc aatcacttct
     6061 cgattgacaa gtaagacttg atcgaagtag tggggaatct tgctgaggtc gtggtgaacg
     6121 atgagaaccg tcttcccagc ttttttcaaa tctctcagcg tattcatgat gatttcctca
     6181 ctgacagagt caatcccagc aaagggttca tccaagagga tatagtcggc ttcctgcacc
     6241 aaacatctgg caatcaaaac ccgctggaat tgacctccag acagttgact aatttgacgt
     6301 tcagcgtagt cagctaggcc gacgatttca agggcctctt gcactttctt ccaatgttta
     6361 gcctttaaac ttcgaaagag aggaatagag ggaaatagtc ctaacgagac gcattccttg
     6421 accttgatgg gaaagttgta gtcgatattg attttttgtt cgacataggc aattcggtgt
     6481 aaggattttt taacttcctt gtcatcgaga aatgcctgac cttgatgtgg gataattccc
     6541 agcatacctt ttaatagtgt tgatttccca gcgccgtttg gaccaatgat gccggtaatt
     6601 gttggtccat ggagcactag tgaaatatcc ttaagtgcca acgtttcttt gtaggagaca
     6661 ctgaggtttt cgatacgtat cataaacttg tattcctcct gtctcttaat atacattaaa
     6721 aaaaaaatta agtcaagtta atttttgaaa aaattaaaat aataactgaa aaataga
//
LOCUS       DAVBCG010000100         6750 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBCG010000100 DAVBCG010000000
VERSION     DAVBCG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.100"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(117..1541)
                     /locus_tag="VQ495_001624"
     CDS             complement(117..1541)
                     /locus_tag="VQ495_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2531559.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2085..4364)
                     /gene="pulA"
                     /locus_tag="VQ495_001625"
     CDS             complement(2085..4364)
                     /gene="pulA"
                     /locus_tag="VQ495_001625"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2531560.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4476..6434)
                     /gene="ligA"
                     /locus_tag="VQ495_001626"
     CDS             complement(4476..6434)
                     /gene="ligA"
                     /locus_tag="VQ495_001626"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2531561.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6527..>6750)
                     /locus_tag="VQ495_001627"
     CDS             complement(6527..>6750)
                     /locus_tag="VQ495_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2531562.1"
                     /translation="FFTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHL
                     SSFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         1983 a         1492 c         1227 g         2048 t
ORIGIN      
        1 ctgtactatt ctagtttcaa tatactataa taccaaaaag ttcgtatttt ttctataatt
       61 attattattt tatagcaaaa aataaaacca ggaaaacaat ttcctgattt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaattcct
      181 tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag gagattgccc
      481 tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttcaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa ttttttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg caaataagcc gcacgttcaa ttgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaataacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaagcttg atttttcaaa gtatactcaa taaaaataaa aaaatccact
     1681 acgtcaaggc gaggctaatg tggtttgaag aaattttcga agagcgtgaa tgagtatcat
     1741 ctatagtaaa ataaaaaaac tgaacaattt ggttggggac agccaaacca atttctcaca
     1801 atgtttcaga aacaagggtg tgctattcca atttcagcct actataactg tcatagattg
     1861 ctgaaacaaa gtctaggtaa aagtcttcat aataaaaaga cctcctatca aatgttcaaa
     1921 aactttgata ggaggtcttg tttcgtgaaa atatttatca aattttctat acaagtgagc
     1981 tgttagccag gttctttcta ttctttcaat ttcaatgaat ggatttttta ccaatactca
     2041 taactgggaa tttttctgtc taaaaatagc gagatagatg gtatttataa aacactcaag
     2101 acagctagac taatatcatt taaaacatta tcttcttttg agcgactgtt ggttaccaac
     2161 atagctaaat ttcctgcatt ttcaaattga tagggttctg atttagcatt cacaactacc
     2221 aagaggtgtt ctttgccgtg aacttcatag ataaggcagc cgctatgttc aatcgcagaa
     2281 tgcacaaaga catgatggta aatttcatca tagctagagt aagaaaaggc accagttttt
     2341 gtcttcaatc ggatgacttg acggataaac tcaatactgt cttgacgctc attaatcaag
     2401 ttccagttca cttggttcac actgtcagga gcattatagc tattcatcgc acgctctcta
     2461 tcatcatggg tcaactcacc attttcacca gtcgcaacca gtttggtacg accaaattct
     2521 tgaccgattt ccataaaggc catcccctgc atgagcagat tcatggctgt ggcagtttcg
     2581 accttgcgca tgatttgctc tgaactttgg tctggatgaa gggttgccaa taaatcgtga
     2641 agattgtaat tgtcatgggc ttctacatag ttaagcacct gatttggatg tgtatagctt
     2701 cctaattcac gacttcctag gattgcttta gctagaattg gctctgtcgc agcaccactg
     2761 acaaaacctg acttgatagc accataaact tctccccctt tgacagcatc gcgctgattg
     2821 tcattaaaga aaccaatatt tggcatctgg taggcattgt ccttcttggc cttatcataa
     2881 ggggcaagac ctgttcccat atcccatcct tctccataga ggataatgtt ggagtcaatt
     2941 tcatccaagc tttgacgaat catctgcatg gtcttgacat catgaatccc catcaagtca
     3001 aaacggaagc cgtcaatatt atattcctgc acccagtata gaagagaatc aatcatatac
     3061 ttgcgaaaca tttcgtgttc actggctgtt tcatttccaa cacccgttcc attctggaag
     3121 gtaccatctg gattcatacg ataatagtaa tcagggactg ttgtttggaa tggtgcatca
     3181 acaactgaga aggtatggtt atagactaca tccataatga ctccaatacc cgcatcgtga
     3241 taagcttgaa ccatcacctt caaatcacga atgacctgag ctggatcatc tggattagtt
     3301 gaaaaactag tttctggcgc gttatagttt tgtggatcat aaccccagtt gtaggttaca
     3361 tttccatcct catcgtattc tttatgacgg tctgcaattg gttgcaattg aacataattg
     3421 tagcccagct tcttgatgta atcaaaagca gttgactggc cgtattggtt aactgttcca
     3481 gcctgagcag cacccaagaa agttcctcga agatgttcat ctacacccga tgtaggtgat
     3541 ttagtcaaat cacgaatatg catttcacag ataactgcct tacatggatt ttccaagcgc
     3601 caagtagcct ccgaaccgtg cttaacctcg aagttttcaa cttgcttttc tacatggctc
     3661 agaatagctg aacgtttgcc atcagggctg gtcgcgattg tataaggatc acgtgtcagt
     3721 gtttggtgat gagggaattg gacttgatac tgataagtct tacctaccaa atcttcttca
     3781 acatccaaac tccagacacc gattgtattg tccttatgat tataagagta gctattgcct
     3841 cttttcatct caaaagtctt ccaaacgggt gcatcattag cagctgattc ataaacgaca
     3901 acttgcactt ctgtcgctgt aggtgaccag agggaaaaat gagcctgatt gtcctctaca
     3961 cggcaaccca attctccttg gtaaccccaa tgatgatcaa aactagcact gttaatggcc
     4021 ttatcaaagg caaaaggatt ttgattttta tagaaaggac tggcaatagc aggattttca
     4081 gagtaataaa tcctatcatc gccttccaaa atccagacct ctgttaatag gggatagtga
     4141 ttaaaacgga tagaatattc tttactagtt tgacctgtat gaaccacaaa attcaagctt
     4201 tctataacat gtgaacttgg gtgttcaaag ctaaataaag ctccaaaata atcttctttg
     4261 taggttagca aatcaattcg ttgatcctga ctttttacaa aggagcaagt gtcatattct
     4321 ccattcttac gatggtaatg aatgcgcata gggtagttat acatttttta tttttccttt
     4381 ttactttgtt tctatttcac taataaattt ttgtcaatct cgtctcaatt aacagacata
     4441 gtcatattct ctaaactctg tttttaaacg atccattaca aactttctag ccatgcctca
     4501 tctctgacct ggataccaag ttcttgtgct ttttgcagtt tacttccagc gtctgcacct
     4561 accacgacga ggtcggtctt tttagaaaca ctacctgtca ctttggcacc cagactttcg
     4621 agtttacttt tagcttctga gcgcttgagt cgttccaatt ttcctgtcaa taccacggtc
     4681 aaacctgaca aggccgcatc cgctactacc gtctgtcctt tatagtccag attgacccca
     4741 gtttctttca attctctgag cagaatttca gagccttctg tcgcaaaata agtctgaaga
     4801 cttttggcaa tcacgccacc tagactttca atactagcca cttcctctga atctgcctga
     4861 gacagatttt caattgaatg gaaatattga agtaaaagct gactagcctt gcttccgaca
     4921 tgacgaattc ccaaaccaaa taagagcttc tcggcagaat tttcctttga tgcttggata
     4981 gcctgataca gtttagcagc ggacttttcc ttaaccccct ctaaaaggag gaaatcctct
     5041 tcttgcaaac gataaatatc cgccacatcc ttgactaaat tagcagcaaa aagcttctca
     5101 acaatagatg gaccaaggcc tgtaatattc atagcatcac gagaagcaaa gtgaatcaag
     5161 ccttccatga tttgagcagg gcaacgcgga ttgatacaac gtagggccac ttcatcttca
     5221 aagtgcaaca agtcagagtt acaacttgga cagtttgtag ggatatctag tttttcttca
     5281 gaaacccgtt tggactctac cacacgtaaa acggcaggga tgatgtcacc agccttatat
     5341 acaatgaccg tatcgtcttt tcggatatct ttttcagcaa tataatctac attgtgcagg
     5401 gtcgcacggc taacagtcgt accggcaagt tgtactggtg ttagattagc agttggagtt
     5461 acaacaccgg tacggccaac tgtccagtca actgataaga gttgagcttc tttttcttca
     5521 gcagggaact tgtaggctac tgcccacttt ggagccttaa cggtaaaacc aagttcttct
     5581 tgacttgcta ggtcgttgac cttgattacc actccatcaa tatcgtaagg cagattttcc
     5641 cgttcctgtc ctacttcttg gataaaattc cagatttcat ctatgttttc agccaagatt
     5701 cgcttaggat tgaccacaaa acctagttgt tctaggtact tcaaaccctt ttcttggcta
     5761 tcacgagttg aagggctggc ttcttgatag agaaacgttg caagattacg cttggcaact
     5821 actgctgtat ccaactgacg cagagttcct gctgccgcat tacgaggatt agcaaattca
     5881 ggctctccat tttcttggcg cacttggtta acttggtcaa aggaagcgcg tggcatgtaa
     5941 cattccccac gaactgtgat atctagttct tctggcaaag tcaaagggat gtccttaaca
     6001 cgcttgaggt tttctgtgat attttcacca attgaaccat ctccacgtgt taccccagca
     6061 accaaaatcc ccttttcata agtcagcgag atagataagc catcgatttt cagctcacaa
     6121 atataggtcg gatgagccac ttccttacga acacgcgcat caaaagcatc tagctcctca
     6181 cgtgaaaaag catcctgcaa actataaaga ggatactgat gactgtattt ttcaaaacca
     6241 tctaaaacct tgccaccaac acgatgagtc ggactgtctg ctagcacttg ctctggataa
     6301 gcagtttcta actcgaccaa ctcacggtaa aggcggtcat actcactgtc tgaaaccgag
     6361 ggattatcgc tggtatagta ctcagtcgca tagcgattga gcaaagcgac taactcattc
     6421 attcttttat tcataagacc attttaccat aaaacaagcc ctcctcacaa acgagaaggg
     6481 cggaaaaaac acttagtttg aaattatttt tgaaactcaa gcaaccttat atcaattttt
     6541 caaaatgagt tcgaacataa ataaacgata tacaagacaa gatgataaca ccacttccaa
     6601 ttatcaggaa agaagagaga tgtacacttg gcaagactgt cataaatcct tttgcaatag
     6661 gcataaatag aatagctaag gtaaaaattg tactcagtac tcttccaaga aattcgctct
     6721 caaccttggt ttgtacttga gtaaaaaagt
//
LOCUS       DAVBCG010000101         6726 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBCG010000101 DAVBCG010000000
VERSION     DAVBCG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.101"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(81..1223)
                     /locus_tag="VQ495_001628"
     CDS             complement(81..1223)
                     /locus_tag="VQ495_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2531563.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(1389..2009)
                     /locus_tag="VQ495_001629"
     CDS             complement(1389..2009)
                     /locus_tag="VQ495_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2531564.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(2013..3293)
                     /locus_tag="VQ495_001630"
     CDS             complement(2013..3293)
                     /locus_tag="VQ495_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2531565.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(3354..4838)
                     /locus_tag="VQ495_001631"
     CDS             complement(3354..4838)
                     /locus_tag="VQ495_001631"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2531566.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(4914..6191)
                     /locus_tag="VQ495_001632"
     CDS             complement(4914..6191)
                     /locus_tag="VQ495_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2531567.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(6298..6381)
                     /locus_tag="VQ495_001633"
     tRNA            complement(6298..6381)
                     /locus_tag="VQ495_001633"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6345..6347),aa:Leu,seq:caa)
     gene            complement(6391..6462)
                     /locus_tag="VQ495_001634"
     tRNA            complement(6391..6462)
                     /locus_tag="VQ495_001634"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6428..6430),aa:Gln,seq:ttg)
     gene            complement(6476..6548)
                     /locus_tag="VQ495_001635"
     tRNA            complement(6476..6548)
                     /locus_tag="VQ495_001635"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6513..6515),aa:His,seq:gtg)
     gene            complement(6563..6633)
                     /locus_tag="VQ495_001636"
     tRNA            complement(6563..6633)
                     /locus_tag="VQ495_001636"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6599..6601),aa:Trp,seq:cca)
     gene            complement(6638..6718)
                     /locus_tag="VQ495_001637"
     tRNA            complement(6638..6718)
                     /locus_tag="VQ495_001637"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6682..6684),aa:Tyr,seq:gta)
BASE COUNT         2027 a         1592 c         1190 g         1917 t
ORIGIN      
        1 acaaagaaaa aacgaacgag aatcgcttcc catcacgaaa gctagatctc attcgtcaaa
       61 atgatgttac caagtcgggt ttatccattc attgaaacgt gaacgtgatc atagtgattt
      121 tctgtcacac taccacggtc tggcattggg ttccaagtgt tagctggccc atatttgcta
      181 tcgaatggag catagaaacg ttgtttccag atgatgtaac taatgccacg gctggccatg
      241 ttttgaatag catattccgc aatcttatcc cctaattctg aacgttctgg taccataaag
      301 tcgatagcca aaccttttcc gtgatctcca ctgtctcctg gacgataacc gctaaaggat
      361 gtaatgccaa acaagttagc aatttcttct ttaaaggcag ctgtttgtgg ttgaagacct
      421 gcattttcag attttgctac tgcaagtcca gcataatcgg gcgcagctgg agcctcgtaa
      481 gttgttgaaa ttattttcgt ctcttctggt tgataagtag aaactgctgc tgtagctcca
      541 tttgatgatg ctacttcttt tgcttctgaa cttgttgtag ttgcttccac tgctgatgca
      601 gcttgagggc ttgcttgtgt ttcctgcttc tcagctggag tcgttccctg aggagcttct
      661 tctgcaactg cactgcttgt ttcggccgtt tgctcctctg ggacagaagt gcccgtagat
      721 ggtgccactt cttcagaagc aatcactgtc tttgtaactt ctgaacttga tgctacttct
      781 tttggagctt ctgcaattgg ttgagaaagg tctgcaacct gaacagtttg atcatcaacg
      841 gtcacttgat tagtggtcaa atctgctgtc gcagttgtca cttcttcact agagtctgct
      901 tgaggtgttt ggatttcaac ttctgttact tcttctgctt cattgacagt cgttgtcaaa
      961 acagtttctg ggaaaatcaa gtccatatta gtgattttgt tcagattcgc aagcactgtg
     1021 acatctacac ccaaggcttc tgcaatggtg ctcaaagtat caccatactg tacggtataa
     1081 cttgttttgt tgtccgtttt agtcaaatcg ttttggattt gctcaacact acgtgcagtc
     1141 caaagaactt cttctgcttg agttgccaat actggggcaa atgacaaggc tactgttgac
     1201 gctaataaca ttcttttctt cattctttca aattcctttc aaatgagtac ctgtctatca
     1261 taacacaaaa aatgtagaaa aaaagccctc tcgggcctat ttaacagaac tgtctattcc
     1321 ttgtaacaaa ctagattact tgaaatagtt tgttaggtct ctgtcacaaa actgacacaa
     1381 tctttttatc acttagtctc aaaaatacgg gaaatatctg ctaacgcttg aatcctgtga
     1441 ttcccttcat aagtagactc taaaaagttg atactttgaa tcccactatt ctgggcaaat
     1501 tccacatcca gagtccgatc ccctatataa taagtattat cagaattcaa ctgatactta
     1561 tctagcagat aggtagccgc ttctggactt ggcttccgca caaagccact ctgactggtc
     1621 aaaatctctg taaaatagga ttccaccccc aagtccttga gaatggtaaa agtgttgttc
     1681 cccttatgag tatatataaa ctgctgaatt cctgattcgt ctgcccaagc tagcacctca
     1741 cgcgcacctg gcatcaaaac tacctgagca ttcttctcag ccagactctg ggcacgcacc
     1801 tgatttagca cctcaacatc cagatttcta tcttctgcca cccgcacaag caaattttgc
     1861 accgaatact tgaagataaa ctctctcacc ttctccttat cataaggaat agaaaactga
     1921 gcaaaagtct cctcaatccc tgataaaatc gcttcgtaag agtccaataa agtcccgtct
     1981 aaatcccaaa taaaagctgt tttttgcatt tcctatcctt tcaagctcat atagcgctgg
     2041 taacgatagc gtagaaataa ccagcgaaaa ccattatcca agagagaccc tgcccaaata
     2101 ccaggcaagc cccaaccaag cacaataccc atcagatatc ctgtcccaat gcggatacac
     2161 cacatgccta tacttgtcgc ataaaaaggg aggcgtgcat ttcctaatcc ctgccagact
     2221 gccgtataga tgactgttcc tgtcgtcata ggggtaccaa gtagtgaaaa cagtgtcact
     2281 agaacactag cctccaccgc tagagaatca gtcgtataga gatgagttaa tggtacaccc
     2341 aagacatata tactaaagga caggggcaac atgaggaaca gagaaagcca aaaggtttgt
     2401 ttactcaaac tagcaactct tttccaatca tcctctccaa ctgctcgggc caacagcatg
     2461 accgttgccg tagcgacacc aaaggcaggc atatagttaa actgggtcaa gacttctccg
     2521 attgcattcc cagcaactgc ctccgtccca aaagaaacga ccaaggcaat gatcactaca
     2581 tctccagccc tcatcataag tcgctctcca gctgctggta aagccaaggt caacagttcc
     2641 ttatctaaac caaaagttag cttcccataa ggcagtttta attgtgacca caaaatcaca
     2701 agaccaacca aacgagacag aattgtcccc caagcaacac cagctatccc catatccaga
     2761 acaaaaatag ctagacttga aaaaagaata ttcaaggcat tggataaaaa actaacatag
     2821 agaggcagac gtggattatg cgttgcacga atcaaggctc ccaaactagt cattaaacct
     2881 aagagaacaa tcgatccgcc taccaaagat agatacagtc caccactctc agctacatcc
     2941 ctctccgtcc ccaaaagtcc tatcatctct ttcccagcga agatggacaa aaatcctaaa
     3001 aggaaactta atagtaaggt aatcttcaac gcctcagtca catgataggc caacttcgac
     3061 tggtctttct gccctatgct ttttgaaata acactggaaa tagcagctcc cagagcgatg
     3121 aaaatcgcct gataaatggt gataatatta ccagctactg aaacccctga aatagctatc
     3181 aatcctaaat gagcaaccaa ataactgtcc accattccca ttagcatctg caaaaagttt
     3241 tcacccatag ctggcaatgc aatattaaga atgtctttat ttttcttaaa caatctctcc
     3301 tcctgatgaa aagaaactca gttggtttcc caaccgagtt tgctccctct gtcttaaagt
     3361 cctaaataag cctcaaccgc tgcttgcatg tcagcagctg ccactgttgt cttgtgacga
     3421 attggagcta tttcaagccc atcaactgct ggtggcactg caacgcctga gatttcatgt
     3481 aattgagcca aggcttcaaa gtctgtcaaa cctgcttttc cagttacagc ttctactgca
     3541 actactggga acttgtatgg actagctgtt gaagcaatca ctgtcttagt tacatctcca
     3601 gtaaccgctt ggtattttct ataaactgct gaggcaaccg ccgtatgtgg atcctcaata
     3661 taagaatctg actcataaac acgcttgatt tctgccgctg tttcttcctc agtcgcatat
     3721 tcagctgcaa agaggtccaa aatctctgca tcaaagtctg tcaacttata ttgtccttgc
     3781 gtgttcaagg cattcataag ttcagctgtc ttttcagcat tatttcccaa aagatggaaa
     3841 atcaagcgct ccaagtttga agataccaag atatccatag atgggctggt tgttacctta
     3901 aactcacgtt ttttgtcata gacacgtgtt ttaaagaagt ctgtcaagac attattgtca
     3961 tttgaagcac agattagctt gccaactggt aggccgattt gtttggcata aaaggcagcc
     4021 aagatatttc caaagtttcc tgttggtact gtgaagttaa ccttttcacc agctacaatt
     4081 tcaccagtct taaccaattg agcgtaagca taaacataat aaacaatttg tggcaccaga
     4141 cgaccaatgt tcatagagtt agctgatgaa aattgcaact tgttggtagt caatttttca
     4201 cgaagagcca cgtcgttaaa catgtgcttc acatttgttt gcgcatcgtc aaaattacca
     4261 tcaatagcaa taacatgagt attgtcacca ttctgagtgg tcatttgcaa ctcttgtacc
     4321 ttgctgacac caccctttgg ataaaagacg ataatctcag taccaggcac atccgcaaac
     4381 cccgccatag cagctttccc cgtgtcacca gatgtcgctg tcaagataac aatcttgttc
     4441 tccaaaccat gtttcttagc agcagtcgtc ataaagtatg gcaaaataga caaggccata
     4501 tccttaaagg caatcgttga accatggaaa agttccaaat tgtattgccc atctaatttc
     4561 actaatggtg caatagctgg agtatcaaat ttgctatcgt aggcattgtt gatacagtag
     4621 tccaactcct cagctgtaaa gtcatctaaa aatgctgaca aaactagctt agcaacttcc
     4681 tggtaagaag catctttcaa tttgtcaaag tccaaatcta cctttggata agtaaccggt
     4741 gtaaacaaac cgccgtccgt cgccaaacct tgcaaaattg cttggctggc agttactgta
     4801 ttgttggcat cacgcgttga ttgataaact aatgtcatta ctctctatcc tttatctata
     4861 gtctcgtcca ttatatcatg atttttcaga aaattctccc tttataagag aaattatagg
     4921 ctcaattctt ctctcaaagg ctgtaaatag gtcatttctt ccttatttct caccactagt
     4981 ccctcctcag ctagtaggag taagtctttg gaaaattcaa taatcgtagt ttcttcctca
     5041 tctgtaagat ttttcttaga aaattgtctc cttaaagact tgtaatcata accaaatact
     5101 ttaaagaaag gtgctgtttc taagtaagct tctaacttgt ctaaattaac caataatccc
     5161 aagtgaaaag ctgcagaagc aaaagtccta tcaagtggct gtgtacacac actacgaaac
     5221 tcaactgttc ctcgagtcgt taaatcttgg tactggtaac tacgatgagt ttcaaaatcc
     5281 ttctcttggg ggtaaataat aacctcatcc ccattcagag caaatgcttg gatttcggac
     5341 gtagccaaat agtccccagc ctgaatagga taaaaataat aggtctgccc atcacgttcc
     5401 gcagtaaaaa tcgcagaatg atttagatag tcaaaaaaat cagcttcatc attaaggagt
     5461 ctagcattga ccccaacatt ctctggatag ataccatgca tagattcttc ccagaaaata
     5521 tcccttgaaa ttttcgtatc ccaatccgca cccgaaaatt cagagtttgc aaataaataa
     5581 gccttagccg cttcaatttg agtaaaagca ttaatcaccc gtaagtagtt ggtttttgaa
     5641 atatccagct gaacctggct cccacagata aaagtaccat attcagggaa atgatgtaaa
     5701 tctgatttaa taatatttct actcaaattc aaataatcca tcaacatctg atagcgtgga
     5761 taagccactg gacaattctc atttttatcc cagttgggat ggataccaca gccaacaata
     5821 gcatgatttg attcagctaa ctttctctga attacattca tataattatt gaaacgattt
     5881 tcgacctctt gaatcgtttc agccttacca aatgcaaact caatcgttgt ataggaaact
     5941 tcaaataaaa tagcatcctg acttatcgga tctactaact ggatcagatt gccaaaatca
     6001 tctacctttg ctacggtgag atccaaagta gaaactaaat aatgaaatag atcctttata
     6061 acttcaacat ctgtagcatc cccttctaaa cttgcaacag gatactccaa ctcaattccg
     6121 acaaacaatt caggattttc tttaatattt ttcaaatacc gatgttttaa taaatcgata
     6181 gaacaagaca tactacccta cactttctta atctaaataa aatttgaata actataatta
     6241 taccaaaaat aatataaaaa aggaacgaag aataacaacg ttcctaacac tatactatac
     6301 cggcggccgg ggtcgaaccg gcacgtcctt gcggacactg gattttgagt ccagcgcgtc
     6361 tgccaattcc gccacgccgg caaatagtaa ctggggtagc tggattcgaa ccaacgcatg
     6421 agggagtcaa agtcccttgc cttaccgctt ggctataccc caataatata aaataggcga
     6481 gtgatgggga tcgaacccac gcatgccaga gccacaatct ggtgtgttaa ccacttcacc
     6541 acacccgcca taattctatt aacacgggca gtaggaattg aacccacact gaaggttttg
     6601 gagaccttag ttctaccttt aaactatgcc cgtaaaatgg aaggggaggg attcgaaccc
     6661 ccgaacccga aggagcggat ttacagtccg ccgcgtttag cctcttcgct acccttccaa
     6721 aatata
//
LOCUS       DAVBCG010000102         6684 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBCG010000102 DAVBCG010000000
VERSION     DAVBCG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.102"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            244..1110
                     /locus_tag="VQ495_001638"
     CDS             244..1110
                     /locus_tag="VQ495_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531568.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            1447..2004
                     /locus_tag="VQ495_001639"
     CDS             1447..2004
                     /locus_tag="VQ495_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET2531569.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            2225..3331
                     /locus_tag="VQ495_001640"
     CDS             2225..3331
                     /locus_tag="VQ495_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET2531570.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            3307..4698
                     /locus_tag="VQ495_001641"
     CDS             3307..4698
                     /locus_tag="VQ495_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531571.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            4695..5873
                     /locus_tag="VQ495_001642"
     CDS             4695..5873
                     /locus_tag="VQ495_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2531572.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            6298..6531
                     /locus_tag="VQ495_001643"
     CDS             6298..6531
                     /locus_tag="VQ495_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2531573.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
BASE COUNT         2353 a          787 c         1193 g         2351 t
ORIGIN      
        1 gaaaggggct gaaagaacta ggggaacagt tgtaattaga aggtgatagg tatgaaaagt
       61 ggcgttgaag ctggataagt ttataaagag tttgaatgaa gaaatgaaaa gtaaataagt
      121 tgatgatgaa ttgcaaaaat agattttata tacaatttgt atctttcaaa tcattgattt
      181 gaattttaga tttaatcatg ttacaattaa aaatgaaatc aaatagaaga ggaagatgca
      241 aacgtgggca cactattagc aacaagatta aaaaatagac gaaaagaatt aaaaatgtct
      301 cagcgagaac tagctgaagg tatctgtaaa caggggcaaa tcagtcgatt agagagcgga
      361 gagtttactc ctggagcaga tttcttgcat gcccttgcta agaagttaaa agtcagtatg
      421 gattattttt ttgatgagca ggttgtagag aaagttgagg agttatcaga atttaagaaa
      481 ttagcacaga catttattac aaatcgcaat tatgagtctt tgaaatatat atatgaattg
      541 gagaatgtaa aagctcaccg cctatcttta gcagataaaa tctacatgga atggataaaa
      601 tctctgatag atttttattt ttacggacag aaagaagatg cgattgtacg attagagggg
      661 gtattatcac agttaagtgt atctgatttg aactatctcc aagtttcaaa caccctattc
      721 aatttttatt atgatattgg agatttaggt cgtttcaata aaattcgtga aaaactagag
      781 tatcaagtaa atcacctcaa tttgagtaca atcgaagagt tagaactatc tattaagttt
      841 aactacaacg tttgtcgtta tctatggtta cagaaaaata ttgaagaagc tattactaaa
      901 attacagcta ctataaaaca atgtaaggct tacagaacga attatctttt ggctgatttg
      961 tacttattga tgggaaatgt aagtgaaaat ttttcttcta aaagttcggt gaaagaatat
     1021 tttgagacgg catattttct atacaaactt gaggagaaca tgtcaatgac tctgaaagtt
     1081 gagcattaca ttgcagatat aacagaataa tgaatacggt gttcaagtga gcatcgtttt
     1141 tcaaacctag ttcagataca aaaaagtatc aaataaaaaa aaataaaaaa acattataaa
     1201 aacaattgac aagctaattt tgaagtgata aaataaatgc aagaaaacaa aaggagaggt
     1261 atatgagaag tttgtttaag aaaatagttg ctgtattagt tattggctta attctgttag
     1321 gaattgcgag aacacctcaa gttcataaga tggctagagg gattgatcct ggtcctgcga
     1381 attttattta gaaatcaatt tctactaatt gagtactaat tgagtattaa tgggtaaaaa
     1441 gattgtatgg ataagaaaaa tttacttgta ttggttactg gttcggtagg tgccagtaat
     1501 gtagatacat acttatatta tataaaaaaa ttctataatg ttaaagtgat actttctgaa
     1561 aattctaaaa aatttataag taaggaatta attagttatt tttgtgataa agtttatgat
     1621 gaaatatttg ttgaagaggt agttccgcac gtttttctgc catatgaatc tgacttactt
     1681 cttattttac cagctacggc aaatgtgatt ggcaaaattg ctaatggtat tgctgatgat
     1741 ttagttacag caactgtttt aaactttaat aaaaaaataa ttttttgtcc caatatgaac
     1801 tctactatgt gggacaatca catagttcaa agaaatgtat caattctaaa ggagttggga
     1861 catatatttt tatttgagtc taaaaaaaca tatgaggtag gattgcgtaa agcaatagat
     1921 tcaacatgtt caatgttaca accacagtcg ttagtaaaag aacttatcaa attagaaaat
     1981 attgtccttg aagagggaca ttaaaaacta ctgagaatat taaggagggg aaaaaatgga
     2041 aaattcatca atcgatgtag atatgctgtt ggaagaattg acacaagaag caatggtcgt
     2101 tgttgctgtt gataaggact gttaatttaa acttatggca atatatgaaa ggttactgga
     2161 tgttttaaat tatgcaggca gtagcctttt attatataca aatggataaa gtaaggataa
     2221 tacaatgatt aataaaaaaa tacaacaagt tgttttggaa tcattacaga attttttgaa
     2281 tgggaacttc atttcgcctt gtgtagtcta tgattttggc ttgctggaaa ctgtacttga
     2341 tgaatttaaa aatcaaattc ctgtaacatt caattaccaa cttttttatg ccgttaaagc
     2401 aaattcaaat gagaagatac ttgaattctt agtagataaa attgatggag ttgatgtggc
     2461 gtcattatct gaattagatg tggctaaaaa atttttccca ccaactcaaa tttctgttaa
     2521 tggtcccgca ttttcttatg aaactttata taatctgatt aaaaaacaat ataaagttga
     2581 tattaacttt ttggaacatc ttcaacaatt ttccccaaaa gaatctgttg gaataagagt
     2641 aacggagcca gatgaactta ataatcgtat gagtcgattt ggaataaata tttgcagtga
     2701 taattggact agtaatttac aaaatccttt aattacacga ctgcattttc attttggaga
     2761 aaaagatgat aaatttattg ttaagttaga taaaatatta tttaagttac aagaaattaa
     2821 taaacttaga gaggttagag aaataaatct tggaggcggt tttatgaaat tatttatgga
     2881 aaatcgtttg aaagaatttt ttctatcact tatggaaatc tataaaaagt acgatattga
     2941 tagtactgtg actacaataa tagaaccagg tagtgcaatt acttcatttt ctgcctatat
     3001 gattactagc ccagttaatg ttagtgaggt gaatgagcag caggttatca cgttagacac
     3061 atcaatatac accaatacat tatggtttgt tccgcatatt attacaacgt taaattcaag
     3121 tagtaaagag cgttatagta ctattctcta tggtaatacc tgttatgaac atgacaagta
     3181 taaaatgaaa gtttcgcttc caaggttaac tcaaaatagc agtatagtgt tttttcctgt
     3241 aggagcttat ataaaaagca atcattcaaa tttacatcgt aatgatttta tgcgggaggt
     3301 atatttgtgg acaaaaaact tgacatatta gataaagtta aggaatattt aggaaataaa
     3361 actactcaaa ttctggataa tcaatataaa gaatttttga aacttaatga tataaggcga
     3421 gcgtttggta tttcagaaaa agtattaaac aattctttta attttacgag taaagaattt
     3481 aatgatttaa ttaataacga aaattattta ttcgaatatg catgtagaat tagagaggaa
     3541 tggagaaaaa aatgctttaa tcattcttat cgttttctat gctcacctat aattacagat
     3601 gattttctta acacgaagac attgagaagt agccaaattg aatataaata tgagcgatat
     3661 ttatcgaaaa gttcgatagg cgatagagcg gttgatggct ttgtttcctt caatacttta
     3721 acagctaatg gtatgtctgc tattaaacta tgtcttgaga tattaaactc tattttcttc
     3781 aagaagaaga ttgatttatt atattcaacc ggatattatg aaacaagatt tttattaaat
     3841 aatcttgcta aatcaggtat tagttgctat gaggtaagta attgtgaatt ggataaagat
     3901 aaattttata atgtattcat gatggaaccc aatcgagccg atttaacatt acaaaaaact
     3961 gatttcaaga tagtagaata ttttgttaag tataaaaata attcaataaa agtcgttatt
     4021 ttagatattt catatcaagg ttctaatttt aaattagtag aatttttaga gaaatttaaa
     4081 tttgcgaatg taattatttt tgtggtacga tctttgataa aattagatca aatgggatta
     4141 gaattgacaa atgggggaat aatagaagtg tttattccta atcatttgag aaagttgaaa
     4201 aattttattg aagaggaatt caataaattt agaaattctc acggagctaa tctaagcctc
     4261 tatgaatact gtttgcttga taattcttta accttaaaaa atgattggaa ctattctgat
     4321 ttagttatga aatttacgag taatttttat gctgatataa aagacttgtt catggaaaat
     4381 tctgatattg aaatcatcca tgaagaggga gtaccttttg tatttttaga tttaataggt
     4441 gaaggtaaaa aagaatatga aatgtttttt caatggttaa acttctttta caaacagctt
     4501 ggaatcacat tgtatgctag aaatagtttt gggtttcgga atctaacagt agagtatttt
     4561 ggaattattg ggacagaaag atatatattt aagatttgtc caggtgttta taaagggtta
     4621 agttattatt tgatgaaatt tttattaaaa tctttttcaa atgaatattt aaaaactact
     4681 gatgaggtta atagatgaaa aatttgataa agttgctaat aattagattg attgttaact
     4741 tagcagacag tgtattttat atagtagcat tgtggcacgt tagcaataat tattcttcga
     4801 gcatgttctt aggaatattt attgcagtaa attatctacc ggatttgtta ctaatctttt
     4861 ttggaccagt tattgacaga gtaaatccgc aaaaaattct tataatatca attttggttc
     4921 aattagcagt ggctgtaata tttttattat tattaaacca aatatcattt tgggtgataa
     4981 tgagtctagt gtttatttca gtaatggcta gctccataag ttacgtgata gaagatgtgt
     5041 tgattcctca agtggtagaa tatgataaga ttgtatttgc aaattctctt tttagtattt
     5101 cgtataaagt attagattct atttttaatt cattcgcatc atttttacag gtggcagtag
     5161 gatttatttt attggttaag atagatatag gcatattttt acttgctcta tttatattgt
     5221 tgttgttaaa atttagaact agcaatgcga atatagaaaa cttctctttc aaatattaca
     5281 agagagaagt gttgcaaggt acaaagttta ttttaaataa taaattatta tttaaaacca
     5341 gtatttcttt aacgcttata aacttttttt attcatttca gacagtagtt gtaccgattt
     5401 tttctattcg atattttgat ggtccgattt tttatggtat ttttttaact attgctggtt
     5461 tgggtggtat attgggaaat atgctagcgc caatcgtaat aaaatattta aaatcgaatc
     5521 aaattgttgg tgtatttctt tttttgaacg gctcaagttg gttagtagca attgttataa
     5581 aagactatac tttatcactt attttatttt tcgtttgttt tatgtctaaa ggagtcttca
     5641 atattatttt taattcgttg taccaacaaa tacctccaca tcaacttctt ggtagggtaa
     5701 atactaccat tgattctatt atttcttttg gaatgccaat tggtagttta gttgcaggaa
     5761 cgcttattga tttgaatatt gaattagtgt taattgctat tagcatacct tattttttgt
     5821 tttcttatat tttttatacg gataatggat tgaaagaatt tagtatatat tagaaatgtt
     5881 tatgttcatt caaaagcata atgactataa ctgaaaaaga aaagtgatat ctttaaggtt
     5941 gttcttcttg gtggtgagat tcgtgagaca acccaagctt ttgtcggaaa gattaccaat
     6001 gctttgatgg ataggatgta ctttagcaag atgtttttag tggtaacggt atcgtggatg
     6061 gacgtgtaat aacctcttct ttcgaggagt attttactaa aaaactagcc ttggagcgtt
     6121 ccccagaaac ggacttactc attgactctt caaagatttg gggagaagat tttgcttcat
     6181 ctgttccttg aaaaaagtca cagcagtcat cacagacgat agtactgaac aaaactatga
     6241 agagttagaa atttatacgc aggtgattgt ataaaggatc tggaaataga taagaagttg
     6301 attagtattg acctaggtgg tacaaatatt aagattactg ttctttcaaa tgacggtgag
     6361 attgaaactt tgtggagtat tacaacagat acaagtgaga aaggttctca aattatatcg
     6421 gacatcatca gttctattaa aaataaattg accgaacgga atattcctga tagcgacctt
     6481 cttggaatcg gtatgggaag ttgctcatca tactttcctt gtaaatcata ggggctataa
     6541 actctccgtc tacttgtcct gcaacaattg aagtctgctc aaaacgccgt ccgctaatct
     6601 tttcatagac tttctccctt ttaggagcct agctttctag tttgttcttt gatttttatt
     6661 gagtatacca ctattttact ccct
//
LOCUS       DAVBCG010000103         6659 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBCG010000103 DAVBCG010000000
VERSION     DAVBCG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.103"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            74..796
                     /locus_tag="VQ495_001644"
     CDS             74..796
                     /locus_tag="VQ495_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2531574.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVMDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            1013..2347
                     /gene="pepC"
                     /locus_tag="VQ495_001645"
     CDS             1013..2347
                     /gene="pepC"
                     /locus_tag="VQ495_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2531575.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAK"
     gene            complement(2403..3314)
                     /locus_tag="VQ495_001646"
     CDS             complement(2403..3314)
                     /locus_tag="VQ495_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2531576.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(3338..4141)
                     /locus_tag="VQ495_001647"
     CDS             complement(3338..4141)
                     /locus_tag="VQ495_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2531577.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(4169..5158)
                     /gene="manX"
                     /locus_tag="VQ495_001648"
     CDS             complement(4169..5158)
                     /gene="manX"
                     /locus_tag="VQ495_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2531578.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(5391..6410)
                     /gene="adhP"
                     /locus_tag="VQ495_001649"
     CDS             complement(5391..6410)
                     /gene="adhP"
                     /locus_tag="VQ495_001649"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2531579.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKNLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         1995 a         1376 c         1460 g         1828 t
ORIGIN      
        1 ggtttgaaga gtatgaaacg aaaaggaggt tggtcattgt accagcctcc ttttgatatg
       61 atataatagt tctatgagat tagataaatt tttagttgcc tgtgcggtgg gaagtcggac
      121 tgaggtcaaa aacttgctca aggctgggcg cgtgactgta aatggtaaaa aagaaaaatc
      181 agctaaattg cagattgatg aaaaaataga tgagattcgc tttgatgggc aagtgttgga
      241 gtatgaagag tttgtctact acatgatgaa caagcccaaa ggagttatct cagcgactga
      301 ggatcccaag cacagaaccg ttatggactt gttggatgac ttggcgcgga gtaaggaagt
      361 tttcccagta ggacgcttgg atattgacac gcatggtctt ttgctcttga ccaatgatgg
      421 tcagctggct catgttcttc tttcgcccaa gcgtcatgtg gacaagactt atctggcaca
      481 agtcaaggga atcatgaccc aagaagatgt ggagacattt gctgagggta ttcctctcaa
      541 agactttacc tgtcaacccg ctatactgga gcttgtatcc atagatacag aaaagaatca
      601 aagccaaatc cgtgtgacca ttgcagaagg gaagtttcat cagatcaagc gtatggtggg
      661 ctactgtggc aaggaagtgg tggacttgca acgattgact atgggaactt tagtattaga
      721 tgagaacctg gaacgagggg aatggcgtcg cttgaccaag gaagaattag aaattctccg
      781 cgctaatatt atttaattta gtttaatact caatgaaaat caaagagcaa actaggaaac
      841 tagccgcagg ctgtacttga gtacggcaag gcgacgttga cgtggtttga agagattttc
      901 gaagagtata aattcgaaaa aggtgaggga caatgtcctc gccttttatg ttttttagtt
      961 gcttcctttg tgaaaagagt tataatagac tgtagaataa aaggaggaat ctatgaacgc
     1021 gattcaagaa tcatttactg ataaactatt tgccaactat gaagcaaatg taaaatacca
     1081 agcgattgaa aatgctgcca gccacaacgg aatttttgca gctctggaac gtcgccaaag
     1141 ccatgtagac aacacacctg ttttctcatt ggatttaacc aaggacaagg tcactaacca
     1201 gaaagcgtct ggtcgttgct ggatgtttgc ggctctcaac accttccgcc acaaactcat
     1261 ctcgcaatac aaattggaga actttgagtt gtcacaagcc cacactttct tctgggataa
     1321 gtatgagaaa tcaaactggt tcttggagca agtcattgcg acttcagacc aagaattgac
     1381 tagccgcaag gttagcttct tactccaaac acctcaacaa gatggtggtc aatgggatat
     1441 ggtcgtttcc ctctttgaaa aatacggtgt cgtgcctaag tcagtttatc ctgagtctgt
     1501 ttcatctagc agcagtcgtg agctaaatgc gatccttaat aaattgcttc gtcaagatgc
     1561 tcaaatcttg cgtgatttgc ttgtttctgg tgcagatcaa gtgactgttc aagctaagaa
     1621 agaagacctc ttgcaagaaa tctttaactt tcttgctatg tcattaggac ttccaccacg
     1681 caagtttgac tttgcttatc gcgataaaga taacaactac aaaagtgaaa aaggaatcac
     1741 accacaagag ttctacaaga aatatgtcaa tcttccttta gaagactacg tttctgttat
     1801 caatgctcca actgctgata aaccttacgg aaaatcttac acagttgaga tgttggggaa
     1861 tgtggttggt agccgtgcag ttcgttacat caacgttcca atggagcgct tgaaagaatt
     1921 ggcgattgcc caaatgcaag caggtgagac tgtttggttt ggttctgatg tcggccagct
     1981 cagtaaccgt aaggctggaa tccttgcgac agatgtttat gactttgaat caagcatgga
     2041 cattaaactt actcaagaca aggctggacg tttggactat agcgaaagct tgatgaccca
     2101 cgccatggtc ttgacaggtg ttgacttgga cgaaaatggt aaatcaacca agtggaaggt
     2161 tgaaaactca tggggagaca aggtcggtac agatggttac tttgttgcct cagacgcttg
     2221 gatggacgaa tacacatacc aaatcgttgt tcgtaaggaa ttgctgacag cagaagaaca
     2281 agctgcctat ggagcagaac caattgtact tgcaccatgg gatccaatgg gagccttggc
     2341 taaataaaag catagaaaaa aggaatcaga ttttagaacc tggttccttt ttagttgctt
     2401 gattacatga tgtgaagaac atgtgccaca atacccactg cgaagagggc aaggataata
     2461 gtgattggag atactttttt cttaagtaag tacatgcaaa gtaaagtaag gagtagtcct
     2521 gataatccag gaatcaacat atccaagttt tgttggaaag tagtaacttt ttcaggagtt
     2581 tgagacaatc cttgtcctac ttgtgcgaat gcttcttgga tacctttaga cccttctggc
     2641 aatttatccc aatggatata agccttttca tctagttgaa ctttagaaac atcgaaagca
     2701 aatttaatat ttacccagcg ttgaacaagg acagcaagaa tgaacatccc aaggatagaa
     2761 gctcctttag tgatatcttg aaggatacca ccagacatat ctttagtgat ttctgatcca
     2821 gccttgtatc caatctcttg aacataccac aagaatgaca tacgaatcaa gttccatgca
     2881 acaaagaaga ggagtggccc caagatattg ccagtaaggg caagtgaagc accgagagat
     2941 ccaaggattg ggcgtactgt aaaccagaat actgggtcac cgataccagc aagaggtccc
     3001 atcataccga ttttaacccc ttggatagca gcgtcatcga tttccacacc gttagcacgt
     3061 tcttcttcaa gcgcaagagt aacccccatg actggagcag ctacgtatgg atgagtgttg
     3121 aagaactcaa ggtgacgctc aagagcagcg atttgatctt cttttttagt atagagtttt
     3181 ttgatagctg gaatgagtgt ataagcccag cccaagtttt gcatccgttc aaagttccaa
     3241 gacccttgta agaaggttga acgccaccaa acttttttac gatctgattt agttaattga
     3301 agtttttcag tcatgatgtt ttcagtcctt tcttatctta gtagtcttct aggatatcgc
     3361 cgattgggtc gttagaagtt gcggctcctc cgccaccatt tccaccagtt ttagaaaggt
     3421 gaaggtagat aagagcgata gcaacaccga tagcaccgaa tccgattaga gtaatatctg
     3481 acacagcagc gagaacgaaa ccaagagcga agaatggcca tacttcacga gttgccatca
     3541 tgttgataac catggcgtaa ccaacggcaa cgaccatacc accaccgata gccataccat
     3601 ctttgagcca gtctggcatg gcactaagga tactttgtac agtttcagtt ggtaccataa
     3661 ggagaagagc tgcaggaagc gcgatacgaa gtccttggaa gagtagcgca atgaaatgcg
     3721 cacgctccac agcgccgaag tcaccttttt tagcggcagc atctgcagta tgaaccaaac
     3781 caactgaaat tgtacgaaca atcattgtca agaaaagtcc agctacggca agagggatag
     3841 caaccgcttg ggcaacaccg ataccagtct tggtaaagtc accaccaaga accatgataa
     3901 tggcagcagc gacagaagca agtgcagcat caggagcgat agcagcaccg atatttgacc
     3961 aaccaagggc aatcatttga agcgatccac cgaggataat ccctgcttcc aagtgacctg
     4021 ttacaagccc aataagggta caggctacaa gtggttggtg aaattggaac tggtcgagga
     4081 tgccttcaag acctgcaaag aaggctacaa cgactactaa aaccatagaa ataatagaca
     4141 tgtttaaaat cctttcataa ataatggctt atttgacatt ggctttgtta atcaagtcaa
     4201 acaaatcttt tttagaatca tttggtactt tacggacatc aaattcaaca cccaagtcac
     4261 gcattttttc aaatgtagca acatcttctt tgtccataga caaaacggta ttgaccaatg
     4321 ttttacctgt tgagtgagcc atagaaccaa cgttaagagt cttgattggc acgccgcctt
     4381 cgatggcacg aagggcatct tgaggtgttt caaacaagat aagggcatgt gtttctccaa
     4441 aacgtgggtc ttttgaaatc tcaatcagtt tttgaattgg aaccacgtta gccttgacat
     4501 tacctggagc tgcttgttta atcaattctt tacgaaggtc gtctttagcc acgttatctg
     4561 aagcaacgat gatacgattt gcttttgaat ctggagtcca agcagttgca acctgaccgt
     4621 gaagtagacg tgtgtcaaga cgggcaagat tgattttcaa tttaccgtct ccgataacag
     4681 ttccttctgg gatagcagtt tgggcaactg gagcagctgc agcgcttgca acttcttcaa
     4741 ctggatttag ctcttctgga agagctttga tgccatcttt ggcttcttta atgatattag
     4801 cagcgacttt ttctacacct gcagcagcgt ccatgaggcg ctctgtgtag gcttgaatca
     4861 acatcggtaa gttaagtcct gtgatgatgg caaacttacg ctcaggattt tctcccatca
     4921 cgcgactagc ttggttaaat ggagaaccac tccaaaggtc agccaaaact agaacctcat
     4981 cttctgcgtc aaatgcagca acagcgttat taaacttagc gtatagatca tcaggacctt
     5041 catttggcat aaaggtcaca acttgaacct tttcttgttc accaaagatc atagatcctg
     5101 actgatgaat acccgcagca aattcgccgt ggctcgcaat aatgattccg atactcatta
     5161 ttgtcattcc tccttttttg ttttagtttt catttaagaa aactttaaga ctgtttaagt
     5221 ataaaccgtt ttcataaaaa aagcaactat ttatcataaa aaattaaaaa atttttattt
     5281 ttgtatgttg atatgttgag tttttgagga gtaatcttga attcttattt aaaatttaat
     5341 ataaaaaagt tggaagaagc ttccaacttt taaaataggt ttctattaga ttagtgggtg
     5401 aagtcgagta ccatacgtcc ttggatttgg cctttttcca tttcgtcgaa aatggcaaca
     5461 gcatcttcta ctggacgttt ttgaacaact gggactacca gaccttctgc accaaattgg
     5521 aaggcttctt ctaagtcttt acgagttccg acaagagaac cgatgacttg gattccatcg
     5581 aggactgttt taacgatgct gagttccatc atttcagaag gaagaccaac agcgacgacg
     5641 cgaccaccag cacgaatgga gtcaacagcc tggttgaagg caactttaga cacagcagtt
     5701 acgacagctg aatgagctcc tccatcagtt ttttctttaa tgagtccagc tacatcttca
     5761 acttcgaggc cgttaatcac aatgtcagcg cctacttctt ttgcaagggc aagtttgtca
     5821 ttgttgatat cgactgcgat aacatgagca ttgaatactt ttttagcgta ttgaacagcg
     5881 aggttaccaa gtccaccagc accgtaaaga acaacccatt ggcctggttc aacttttgct
     5941 tctttgatag ctttataggt tgttactcca gcacatgtga tagaagaagc ttgggctgga
     6001 tcaagtccgt caggaacttt gacagcatag tcagcagtta cgatacattg ttcagccata
     6061 ccaccgtcta ctgagtagcc agcatttttc actgtacggc aaagggtttc gcgaccagtt
     6121 gtacagtatt cgcaagtgcc acatccttca aagaaccaag caacgctgac gcggtcaccg
     6181 actttaaggt ttttcacatc tggagcaatc tctttaacga taccgatacc ttcgtgccca
     6241 agaacacgtc ctgggacttg accaaagtca ccatgagcaa cgtggaggtc ggtgtggcaa
     6301 acgccacagt attcaacttc tacaagtgct tccccagttt caagtggacg gagtactttt
     6361 tcttcaatag caacaccagt gctttctgga tttacaacaa cagctttcat agctatcctc
     6421 cttgatattg accgagaaca gggacaacgg gactggattg attttttgtc aacagagtcg
     6481 agtgtgacga gctctcttgt atttatatgt gaagtatatc acaaaagaaa gccttttcca
     6541 aggttttgga gacaaaaagt agaacaatcg attaactggt tgatgagact gtgaaaagag
     6601 cacaaagacc agcttgcaag ctggtcatac tcttcgaaaa tctcttcaaa ccacgtcag
//
LOCUS       DAVBCG010000104         6637 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBCG010000104 DAVBCG010000000
VERSION     DAVBCG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.104"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..189)
                     /locus_tag="VQ495_001650"
     CDS             complement(<1..189)
                     /locus_tag="VQ495_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2531580.1"
                     /translation="MEHIILLRGVTPNGKNAIPKMSYLVDILTEADFQQVRTYIQSGN
                     IILESNLALEEIREQVHTL"
     gene            complement(240..1412)
                     /locus_tag="VQ495_001651"
     CDS             complement(240..1412)
                     /locus_tag="VQ495_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2531581.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            complement(1471..2316)
                     /locus_tag="VQ495_001652"
     CDS             complement(1471..2316)
                     /locus_tag="VQ495_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2531582.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            2441..2998
                     /locus_tag="VQ495_001653"
     CDS             2441..2998
                     /locus_tag="VQ495_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2531583.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            3017..3484
                     /locus_tag="VQ495_001654"
     CDS             3017..3484
                     /locus_tag="VQ495_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2531584.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            3573..4202
                     /gene="upp"
                     /locus_tag="VQ495_001655"
     CDS             3573..4202
                     /gene="upp"
                     /locus_tag="VQ495_001655"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2531585.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            4376..4966
                     /gene="clpP"
                     /locus_tag="VQ495_001656"
     CDS             4376..4966
                     /gene="clpP"
                     /locus_tag="VQ495_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2531586.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            5045..5293
                     /locus_tag="VQ495_001657"
     CDS             5045..5293
                     /locus_tag="VQ495_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2531587.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            5394..6554
                     /locus_tag="VQ495_001658"
     CDS             5394..6554
                     /locus_tag="VQ495_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2531588.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         2071 a         1287 c         1386 g         1893 t
ORIGIN      
        1 tagagtatga acctgttctc gtatttcttc gagagctaag ttactttcaa gaataatatt
       61 tccactttga atataagttc gaacttgttg aaaatcagct tctgtcaaga tatctactag
      121 ataagacatt ttagggatag cattttttcc attaggagtg actcccctta gcaagataat
      181 atgttccaaa gtagttcctt tctttttttg gggggattgt tccatttagt aatgtatatt
      241 tagattggat ttgatcccgc cacccagccg gtacagaggg cagaagtgat gttaaagcca
      301 cctgtgtggg cattgatatc cataacttcg cctgcaaagt ggaggccagg taccagctta
      361 ctttcaaggg ttttaggatt gatttccttg agactgactc cacccttggt aacaaaggac
      421 tttgcaaggg acatttttcc agttacagga attttaagtt ctttaatgga ctggacaagt
      481 tgttctcgtt ccttttcagt cagttgtttg actttttcag gatatccttg tacaaaaaat
      541 tcggccaagc gttctggtaa caaggttttt aaagcgtttt tcaaggattt ttcccgattt
      601 tcttctagaa atgtaaccaa gtccttctca gaaagttgag gcaaaacatc gagtgagaga
      661 acctccccac ctttgacaaa gctagacatg cgtagggcag caggacctga caaaccaaag
      721 tgggtaaaga gtaaatcatg agtgatgaca tgcttaccat aacttagggt cacatcgtcc
      781 aaagaaatcc cttgtaaggc tttatgtgga aaatctgtta ataaaggact ttcagcagcc
      841 tcaagatcgg tgatggtatg cttaaaatgg cgagcaatct cgtgaccaaa accagtcgaa
      901 ccagtcgaag gataagactt accacctgtt gtgacaatga gtttctcaca agtgaaggtt
      961 tgatccgctg acttaaggac aaactggtca tctacttttt taacagaaac gatttctatt
     1021 tgagtagcaa cttgaccacc tagttcggtg attttctttt ccaaagcttc gataatagtc
     1081 cgagacttgt cactggctgg aaagacgcgt ccgtggtctt cgaccttaag tttaacacca
     1141 ttttctgtaa aaaagttgat gatgtcatga ttatcgaact gggagaaaac actgtaaaga
     1201 aagcgtccgt ttccaggaat tccagctagc aggttgtcta agctaccatt gttggtcaca
     1261 ttgcaacgtc ccccaccagt cccagctaat ttttttccaa gtttccgatt tttttcgatg
     1321 aggagggttt tctgtccata aaagctactg gaaatcgtag ccatcatacc agcaggtccc
     1381 ccaccgatga caatagtatc aaaatgtttc atagctctat tgtaccacaa aaaaacaaga
     1441 gatgatggtc acctcttgtc aagaatacaa ttaatcaatt tcatagccca tcagcaaacc
     1501 gccatcttct gcatagaaac tgcagagacc agaggttggt agaattttaa tatccgcttg
     1561 tgggaaggtt tcacggattc gctctgagag ctgttgacaa catttttcgt tattgcgttg
     1621 ggccatgaca atacggccac cagcatatcc agcttttact aactcatcat aggcagcttg
     1681 aactgatttc tttgatcccc ttgctttttg tagcaattcg agagttccag tttcactagc
     1741 ttttccgacc atacgaatgt tgagaaggcc aacgaccgta ccgataagct tgctcaaacg
     1801 gccgttcttc accaagttat cgactttggc taggacaaag agcaacttag ttttttcttg
     1861 ataggcggtg atagcttcaa ccacttcttc aaaagacaag ccctggtcaa tcaagtcatt
     1921 caatttttct acgagtaggt caacttcacc accagcagat aaactatcaa tcacatgaat
     1981 cttagtgtca ggatggtctt ccagataaat attctttgct agttgagcac tattgtgact
     2041 gccagaaagg gtacctgtga tggttactag gaaaatgttt ttggcacctt caaatgctcg
     2101 caaatagtca tctgggcttg gacaagccga ttttgaagct tctgcagttg catacatggt
     2161 ttccatcatt tggtcaatat cgagactggc gtcatcaaca aagacctgat cagctacttg
     2221 aatggttaag gggacactta caaaggttgt gttaatagct ggtgttggca gttgacgata
     2281 atcacaacca gagtcagcaa taatcttcca agtcatagaa attctccatc tttgtcagtt
     2341 atacattgac aaaggttctg tcttttttta caattatatc atgaaaaccc ttgaaacaaa
     2401 agcatcatcc gtctgttgtg acaagtagaa agaaaatgtt atgtctgaac gtagaatctc
     2461 tgaaaagtct cttgaaaatc tcagaaaatc aaaccaagaa tccaatttat taaccagaga
     2521 agccattgaa acagccctct tgcaactctt ggaaaaaaag gaactgacca agattagtat
     2581 ttctgaattg gtcaaacgtg caggtgtttc gcgtgcggcc ttttatcgca attatgactc
     2641 caaagaggaa attttagaga gcgtctttaa acgaactgtc cacaatatta tggaacagct
     2701 gcatcattac gatttaaaga cagaccttta tttggtttgg gttcaccttt tccgggaggc
     2761 cagaaaggaa gctagagtaa ttcaattggc cttggattac catctggaaa aaatctttgt
     2821 ccaagccatg caggaatttc tagaaaaata ccatgggaaa tcaaaaggtg tcagctctta
     2881 tcttcattcc ttctggagct cagccatcgt ctctgtcctt ctaaaatgga tcaaggatgg
     2941 catgaaggta ccagctgaaa agattgcaga tttaggttta ccatttttta aaaaatagag
     3001 aaaaaggaga aaagagatga ctgaaaaaag actggcctgg gatgagtatt ttgcagccca
     3061 agctctacta attgcgaatc gttccacttg taaacgtgct aaagtgggcg cgattctggt
     3121 aaaagataat aaggttattt ccactggtta caatggttcg gtgtcaggga ctgagcattg
     3181 tattgatcac gaatgtttgg tcattgaagg ccactgtgtt cgcacccttc acgctgaggt
     3241 caatgctatc cttcaaggtg ctgagcgtgg tgttcccaaa ggctttacag cctatgtaac
     3301 ccattttcct tgtctcaact gtacaaaaca attgcttcag gtcggctgca agcgtgtggt
     3361 ttatatcaac cagtaccgaa tggacgacta cgcccaatac ctttatcaag aaaaagggac
     3421 agaattgacc catttaccac ttgagacagt acagacagct cttaaagagg ctgatctaat
     3481 gtaaaaatta tcaaaaataa atggtttaga aagattttta acccgttttt tggtataata
     3541 agaagaataa attgaaagaa ggaattccaa aaatgggaaa aattgaagtt attaatcacc
     3601 cactgattca acacaaattg tcaatcttgc gtcgtacaga tacttctaca aaagcttttc
     3661 gtgagctagt agatgagatt gcaatgttga tggggtatga agtacttcgt gatcttccac
     3721 tagaagatgt ggaaatcgaa acaccaatta caaaaacagt tcaaaaacaa ttggcaggta
     3781 agaaattggc catcgtccca atcttgcgtg caggtatcgg gatggttgat ggtctcttga
     3841 gcttggttcc agctgctaaa gttggccaca tcggtatgta ccgtgatgaa gaaacatttc
     3901 aaccagttga atacttggtg aaattgcctg aggacattga ccaacgtcaa atttttgtag
     3961 tagacccaat gttggcaaca ggtggctcag caatcttggc tgttgattct cttaaaaaac
     4021 gtggcgcatc aaatatcaaa tttgtctgcc ttgtatctgc tccagagggt gtaaaagccc
     4081 ttcaagaagc tcatccagat gtagaaatct ttacagcagc cttggatgaa cgtttgaacg
     4141 aacacggtta tatcgttcca ggtcttggag atgctggaga ccgcttgttc ggtacaaaat
     4201 aagatcgaaa agaagaatga cttggaaaag tatttccagt cacgaaagga ggttgggttt
     4261 ttgtttctgt ctaatgaaag cagagcaaaa atttgacctt ttttgaccaa gatattataa
     4321 tagtcttatc ttcagtcatt tgaccaacaa aattaaaact caaaaggaga aatgaatgat
     4381 tcctgtagtt attgaacaaa caagccgtgg agaacgttct tacgatattt actcacgtct
     4441 tctcaaagac cgcatcatta tgctgacagg tccggttgaa gacaatatgg ctaactctgt
     4501 tattgcccaa ctgcttttct tggatgccca agatagtaca aaagatattt acctttatgt
     4561 caatacacca ggtggttctg tttcagctgg tttggcaatc gtagatacca tgaactttat
     4621 caaggcagat gtccaaacca ttgttatggg aatggctgca tctatgggaa cagtcatcgc
     4681 atcaagtgga gcaaaaggca aacgtttcat gcttccaaat gctgaataca tgattcacca
     4741 accaatgggc ggtacaggtg gtggtaccca acaaactgat atggctatcg ctgcagaaca
     4801 cttgctcaaa actcgtaata ccttggaaaa aatcttggct gaaaattcag gtcagtcaat
     4861 ggaaaaagtc catgcagatg cagaacgtga taactggatg agcgcccagg aaacacttga
     4921 atatggcttt attgatgaaa ttatggccaa caattcattg aactaatgat gatagaaggc
     4981 aaactcgact gggtttgctt tttttggtat aatagggaga gatttcttag aaagaggatt
     5041 tatcatgttt gaaaaagtca atcgctctgg cttgattatc tatctttact ataatcgtga
     5101 tgccaaaaaa ctgcaggatt atggagatat tacctatcat tccaagaaac atcgttactt
     5161 acaactctat gttccaactc aagaagtgga gcaattggtc ggacgcttga gcaaggaaaa
     5221 atttattaaa aaagttaggg tttgtcatat ccaagagttg gaaacaccct ttgtgggcaa
     5281 tctttatcga taggaaaacg ttatcatcga aaaagttcaa ggaaaacatt gacaattttc
     5341 tgataattcg gtatattctt aacagactat ttaagaaata aggagacaaa aagatgaaga
     5401 aaaaatttgc cctatcgttt gtggcgcttg caagtgtagc acttcttgca gcctgtgtag
     5461 aagtgaagtc tggagcagtc aacactgctg gtaactcagt agaggaaaag acaattaaaa
     5521 tcgggtttaa ctttgaagaa tcaggttctt tagctgcata cggaacagct gaacaaaaag
     5581 gtgcccaatt ggctgttgat gaaatcaatg ccgcaggtgg tatcgatgga aaacaaatcg
     5641 aagtagtcga taaagataat aagtctgaaa cagctgaggc tgcttcagtt acaactaacc
     5701 ttgtaaccca atctaaagta tcagcagtcg taggacctgc gacatctggt gcgactgcag
     5761 ctgcggtagc gaacgctaca aaagcaggtg ttccattgat ctcaccaagt gcgactcaag
     5821 atggattgac taaaggtcaa gattacctct ttattggaac tttccaagat agcttccaag
     5881 gaaaaattat ctcaaactat gtttctgaaa aattaaatgc taagaaagtt gttctttaca
     5941 ctgacaatgc cagtgactat gctaaaggga ttgctaaatc tttccgcgag tcatacaagg
     6001 gtgaaatcgt tgcagatgaa actttcgtag caggtgacac agacttccaa gcagccctta
     6061 caaacatgaa agggaaagac tttgatgcta tcgttgttcc tggttactat aatgaggctg
     6121 gtaagattgt aaaccaagcg cgtggcatgg gaattgacaa accaatcgtt ggtggtgatg
     6181 gattcaacgg tgaggagttt gtacaacaag caactgctga aaaagcatca aacatctact
     6241 ttatctcagg cttctcaact actgtagaag tttcagctaa agctaaagcc ttccttgacg
     6301 cttaccgtgc taagtacaat gaagagcctt caacatttgc agccttggct tatgattcag
     6361 ttcaccttgt agcaaacgca gcaaaaggtg ctaaaaattc aggtgaaatc aaggataacc
     6421 ttgctaaaac aaaagatttt gaaggtgtaa ctggtcaaac aagcttcgat gcagaccaca
     6481 acacagtcaa aactgcttac atgatgacca tgaacaatgg taaagttgaa gcagcagaag
     6541 ttgtaaaacc ataatagaaa aatgttgaaa tagggaatga gcctttgact cactccctgt
     6601 ttcgatattt aatactcttc gaaaatctct tcaaact
//
LOCUS       DAVBCG010000105         6476 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBCG010000105 DAVBCG010000000
VERSION     DAVBCG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.105"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            53..1015
                     /locus_tag="VQ495_001659"
     CDS             53..1015
                     /locus_tag="VQ495_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2531589.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1192..2199
                     /gene="gap"
                     /locus_tag="VQ495_001660"
     CDS             1192..2199
                     /gene="gap"
                     /locus_tag="VQ495_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2531590.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2266..3102)
                     /locus_tag="VQ495_001661"
     CDS             complement(2266..3102)
                     /locus_tag="VQ495_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2531591.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3228..5423
                     /locus_tag="VQ495_001662"
     CDS             3228..5423
                     /locus_tag="VQ495_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2531592.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5476..5628
                     /gene="rpmG"
                     /locus_tag="VQ495_001663"
     CDS             5476..5628
                     /gene="rpmG"
                     /locus_tag="VQ495_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2531593.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5638..5814
                     /gene="secE"
                     /locus_tag="VQ495_001664"
     CDS             5638..5814
                     /gene="secE"
                     /locus_tag="VQ495_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2531594.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5870..6406
                     /gene="nusG"
                     /locus_tag="VQ495_001665"
     CDS             5870..6406
                     /gene="nusG"
                     /locus_tag="VQ495_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2531595.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2012 a         1128 c         1418 g         1918 t
ORIGIN      
        1 cacggataca gttgggaaag aagctattaa acatttggag agtccttttg ggatgaacat
       61 gtcaaatatt actatagatg ggcaagaaat ggatctgagt caaacgatgc cttcttatgc
      121 tcaagtagtg agtctttatg atgatacaag tatctctgtt agtagtaatg agacggacaa
      181 agtgttggct ggctccctct atactgatac aaatgaacag ggattaacga ttccaagcag
      241 tttactaaaa aattggaatg aacagacagg aaaaaatttg acagctaatg atcttattgg
      301 caaatcagtc tcagccagca ttgtagaaag tgctgccgaa actagtaaga ttgctcaatt
      361 tcaaacgaag attgtacgtg taatcaatga tgaagatgac atggaggaca gcaacagttt
      421 catgctgtct catcaaatgg aaacgatttt gaaagaggct ggatttacga aagctgtatc
      481 ttattttatc ttggaactca aagatccatc acagacaaaa gtagtaacag aagaattaca
      541 gaaaaataag aagtatactg tgctttctca acagagagtt cttgatattg tgattatctt
      601 tattcgtgtt attcagggat tattgattgt gctttcatca caagctattg tggtagcagc
      661 ggttatgatt ggtatcatta tttacatcaa tatcatgcag cgttccaagg aaataggtgt
      721 catgaaggca gttggttatc agaatcgtga tgtcaaagga atttttattt acgaggctat
      781 ctggattgta ggcatcgcct tgctgctggc atttttggtt gcacaagggg tgggaagttt
      841 ggcgaatgcg attgtaagtc acttttaccc atccatcact aaagtttttg aattaaatct
      901 tttatctgtt ttaggaactc tagttttcgc tctattactt ggttatgtct cagcctactt
      961 cccggcgcgt aagattagta aaatggatcc tgtagaaccg ttacgctatg aatagtaagg
     1021 gattccttga tttacagtct taaaaagaaa ctaacataga aaaacaatgg acatacttgc
     1081 aattcaactc aaatagttgt ataataggtt tgttgaaagg tgatttgtag tgattcaagt
     1141 tactcttttc acaataaaaa tttcatgatt ttcataagga ggaaatcact aatggtagtt
     1201 aaagttggta ttaacggttt cggacgtatc ggtcgtcttg ctttccgtcg tatccaaaac
     1261 gtagaaggtg ttgaagttac acgcatcaac gaccttacag atccagttat gcttgcacac
     1321 ttgttgaaat acgacacaac tcaaggtcgt ttcgacggta ctgttgaagt taaagaaggt
     1381 ggatttgaag ttaacggtaa attcatcaaa gtttctgctg aacgtgatcc agaacaaatc
     1441 gactgggcta ctgacggtgt agaaatcgtt cttgaagcta ctggtttctt tgctaagaaa
     1501 gaagcagctg aaaaacacct taaaggtgga gctaaaaaag ttgttatcac tgctcctggt
     1561 ggaaacgacg ttaaaacagt tgtattcaat actaaccacg acgttcttga cggtactgaa
     1621 acagttatct caggtgcttc atgtactaca aactgcttgg ctccaatggc taaagctctt
     1681 caagacaact ttggtgttgt tgaaggattg atgactacta tccacgctta cactggtgac
     1741 caaatgatcc ttgacggacc acaccgtggt ggtgaccttc gccgtgctcg cgctggtgct
     1801 gcaaacatcg ttcctaactc aactggtgct gcaaaagcta tcggtcttgt aatcccagaa
     1861 ttgaatggta aacttgatgg atctgcacaa cgcgttccaa ctccaactgg atcagttact
     1921 gaattggtag cagttcttga aaagaacgtt actgttgatg aagtgaacgc agctatgaaa
     1981 gcagcttcaa acgaatcata cggttacaca gaagatccaa tcgtatcttc agatatcgta
     2041 ggtatgtctt acggttcatt gtttgacgca actcaaacta aagttcttga cgttgacggt
     2101 aaacaattgg ttaaagttgt atcatggtac gacaacgaaa tgtcatacac tgcacaactt
     2161 gttcgtactc ttgaatactt cgcaaaaatt gctaaataat tcttgagttg atagaaagca
     2221 aggctttgtg gtcttgcttt ttatatggaa aaatggatga cacgatcatc cattcttttt
     2281 taattctttt tcaaatgtat ttgaaagggt agtgaaagtt agcttctcta aagtaagtgg
     2341 gtgggtaaag gaaagtcgga aggcatgaag cataagccga cttgtctttg atttactatt
     2401 atagagaggg tcgcccagga taggaagatt atgatgcgaa aggtgcacac gaatctgatg
     2461 ggttcgccct gtctttagct tgcaacgagc caaggaagtc ttgtttgaga attgctttaa
     2521 tctgcttaca tacgtttcag catattgccc attttttgca tcaactattc tttttctacg
     2581 atcatggcga tcacgtccaa ttttgtctct gaaaacaagt tcttttctgt tgatatttcc
     2641 atcaactaga gcccaatatt ctctagaaat ctcttttttc tccaataagc gattgagaat
     2701 gggcaggata aaaggatttt tggcaaagag aactaagcca ctggtttcca tgtccagacg
     2761 atgaacgaca tagcaggttt ggccaacata ggtactgaca tggttaagaa gggcaatttc
     2821 gtttggttga ttaccatgcg ttttcatccc ctctggtttg tttacaataa tcaagtattg
     2881 atcttgataa acttcctgca ctaagtctgg gttgccccaa gggatcgtct tttggggata
     2941 atcttcctcg tcaaaagtca actggcaaac atctccagga tttacgattt cgttccagtg
     3001 gacttcttct tgatttatca aaatatgttt cttgattctc aaaaaatgac ggatttttct
     3061 agggatgagg agttgttcct caagtaattg ctttaccgtc atttgaggta gagaggcggg
     3121 taatgtaaat gtgaattgca tacagatatt gtaacaaaaa aagccctatt tggataggaa
     3181 atagctaaat tcttgtcttc ctatgatgaa gatgataaaa taaacgcatg aaattagata
     3241 aattatttga gaaatttctt tctcttttta aaaaagaaac aagtgaacta gaggactctg
     3301 attctactat cttacgtcgc tctcgtagtg atcgaaaaaa attagcccaa gtaggtccga
     3361 ttcgaaaatt ctggcgtcgt tatcatctaa caaagattat ccttatacta ggtttgagtg
     3421 caggcttgct agttggaatc tatttgtttg ctgtagccaa gtcgaccaat gtcaatgatt
     3481 tgcaaaatgc cttgaaaact cggactctta tttttgaccg tgaagaaaaa gaggctggtg
     3541 ccttgtctgg tcaaaaggga acctatgttg agctgactga tatcagtaaa aacttgcaga
     3601 atgctgttat tgcgacagaa gaccgttctt tctataaaaa tgacgggatt aactatggcc
     3661 gtttcttctt ggctattgtc actgctggac gttcaggtgg tggctctacc attacccaac
     3721 agctggctaa aaacgcctat ttatcacagg atcaaactgt tgagagaaaa gcgaaagaat
     3781 ttttccttgc cttagaatta agcaaaaaat atagtaagga gcaaattcta accatgtacc
     3841 ttaacaacgc ttattttgga aatggtgtgt ggggtgtaga agatgcgagt aagaaatact
     3901 ttggagtttc tgcatcagaa gtgagtctgg atcaagctgc gactctggca gggatgctca
     3961 aggggccgga actgtataat cccttgaatt ccgtagaaga ttctactaat cggcgcgata
     4021 ctgtcttgca gaatatggtt gcagcaggat atattgataa aaaccaagaa accgaagctg
     4081 ctgaagttga tatggcttcg caattgcacg ataagtatga aggaaaaatc tcagattacc
     4141 gttacccctc ttattttgat gcggtggtta atgaagctgt ttccaagtat aatctaacag
     4201 aggaagagat tgtcaataat ggctaccgca tttacacaga gctggaccaa aactaccaag
     4261 caaatatgca gattgtttat gaaaacacat cgctatttcc gagggcagag gatggaacgt
     4321 ttgctcaatc aggaagtgta gctctcgaac cgaaaacagg gggagttcgt ggagttgtcg
     4381 gtcaagttgc tgacaatgat aaaactggat tccggaattt caactatgca acccaatcaa
     4441 agcgtagtcc tggttctaca attaagcctt tagttgttta tacgccagca gttgaagcag
     4501 gctgggcttt gaataagcag ttggataacc ataccatgca gtatgacagc tataaggttg
     4561 ataactatgc agggatcaaa acgagtcgag aagttcctat gtatcaagcc ttggcagaat
     4621 cgcttaatct acctgctgtt gccgctgtta atgatttggg tgtcgacaag gcttttgagg
     4681 caggcgaaaa attcggactc aacatggaaa aggtcgaccg tgttcttggt gtcgccttgg
     4741 gaagcggtgt tgaaaccaac cctcttcaaa tggctcaagc atacgctgcc tttgcaaatg
     4801 aaggtttaat gcctgaagct cattttatta gtagaattga aaatgctagt ggacaagtta
     4861 ttgcgagtca taaaaattca caaaaacggg tgattgataa gtctgtagct gacaagatga
     4921 ccagtatgat gttggggact ttcaccaacg gtaccggtat tagttcatcg cctgcagact
     4981 atattatggc agggaaaact ggaacaactg aagcagtttt caatccggag tacacaagtg
     5041 accagtgggt aattgcttat actccggatg tagtgattag ccactggctt ggctttccta
     5101 ccactgatga aaatcactat ctagctggct ctacttcaaa cggtgcagct catgtcttta
     5161 gaaacattgc caataccatt ttaccttata cgccaggaag tacctttacg gttgaaaatg
     5221 cttataagca aaatggaatt gcaccagcca atacaaaaag acaagtacaa accaatgata
     5281 atagccagac agatgataat ttgtctgata ttcgagggcg tgcgcaaagt ctagtagatg
     5341 aggctagccg ggctatctca gatgcgaaga ttaaggaaaa ggctcaaaca atatgggatt
     5401 cgatagtcaa tctatttcgc taagatgctt gtcaaagcct agctttcttg ttataatgga
     5461 taggatggag gcgttatggc actaaaaaaa gcaagcctag cttgtgcggt ttgtggttcg
     5521 agaaactatt caatcaagat cagcggaaac cccaagccta cacgactaga agtaaataaa
     5581 ttttgtaagc attgtggcaa gtacactaca cacagagaaa cgagatagga gagagcgatg
     5641 cgttttattg gagatatttt tagacttctt aaagacacaa cgtggccaac tcgcaaggaa
     5701 agctggagag attttcgttc tatcatggaa tacacagctt tctttgtagt aattatttac
     5761 atttttgacc agttgattgt ttcaggtttg attcgattta ttaacatttt ttagaagatt
     5821 agtggagtta attacactag aaatcttcta tattatgaaa ggaaatatca tggatagttt
     5881 tgataaaggg tggtttgttt tacaaactta ttctggttat gaaaataagg tgaaagaaaa
     5941 tctattacaa cgtgcacaaa cctacaatat gttggataat attctacgcg ttgaaattcc
     6001 aacacaaaca gtgcaagttg aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt
     6061 tccaggttat gttcttgtag aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa
     6121 cacaccaaat gttacaggat ttgtcggatc acacgggaac agatcaaaac caactccatt
     6181 attggaacaa gaaattcgtg acattttggt atctatggga caaactgttc aagaatttga
     6241 tttcgatgtt gagattggtc aaactgtacg tattattgat ggtgcttttg cagactacac
     6301 tggtaagatt acagaaattg ataataacaa agtgaaaatg attatctcta tgtttggtaa
     6361 tgacacagtt gcagaagtaa acctaaacca aatcgcagaa ttataaccct gagagaggct
     6421 ggagcctctc ttttttgtgc agtttaggag ctaaagggaa cagaatggag aaaatg
//
LOCUS       DAVBCG010000106         6328 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBCG010000106 DAVBCG010000000
VERSION     DAVBCG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.106"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VQ495_001666"
     CDS             136..2316
                     /locus_tag="VQ495_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2531596.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VQ495_001667"
     CDS             2368..3183
                     /locus_tag="VQ495_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2531597.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETLVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            3323..4666
                     /locus_tag="VQ495_001668"
     CDS             3323..4666
                     /locus_tag="VQ495_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2531598.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VQ495_001669"
     CDS             5043..6233
                     /locus_tag="VQ495_001669"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2531599.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
BASE COUNT         1895 a         1157 c         1398 g         1878 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 actagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgatgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtgatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaaaat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgtg tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccacgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactttagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatgtt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaatt gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacta aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccaattg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggagatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttagcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagatgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa actaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgcgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt gacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacat
//
LOCUS       DAVBCG010000107         6325 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBCG010000107 DAVBCG010000000
VERSION     DAVBCG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.107"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            100..300
                     /locus_tag="VQ495_001670"
     CDS             100..300
                     /locus_tag="VQ495_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531600.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            325..741
                     /locus_tag="VQ495_001671"
     CDS             325..741
                     /locus_tag="VQ495_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531601.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            972..1679
                     /gene="ccdA2"
                     /locus_tag="VQ495_001672"
     CDS             972..1679
                     /gene="ccdA2"
                     /locus_tag="VQ495_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2531602.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1690..2247
                     /gene="sdbB"
                     /locus_tag="VQ495_001673"
     CDS             1690..2247
                     /gene="sdbB"
                     /locus_tag="VQ495_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2531603.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2368..3759
                     /locus_tag="VQ495_001674"
     CDS             2368..3759
                     /locus_tag="VQ495_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2531604.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPSWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            3902..4819
                     /gene="adcAII"
                     /locus_tag="VQ495_001675"
     CDS             3902..4819
                     /gene="adcAII"
                     /locus_tag="VQ495_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2531605.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4827..>6325
                     /locus_tag="VQ495_001676"
     CDS             4827..>6325
                     /locus_tag="VQ495_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2531606.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         1931 a         1151 c         1335 g         1908 t
ORIGIN      
        1 ctatatgtac tattctagtt tcaatatacc ataactgctc ctagtcaacc tgcaagtcct
       61 gtcccagctc caagctcagc tccgactcaa gatcctagag tggtagcaag cgcttcggct
      121 agctcaacga gcacacaagc tcaagagcaa gttgacaagt ctgaacttcg tgccttgagt
      181 caagagttag accaacgctt gaaagctttg gcaacagtat ctgatccaaa aatcgatgca
      241 accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt gacagagtaa
      301 ggtctgcata ctgcagtaga gtctgtgaag gctgccttgg actctctcaa agatgtgaaa
      361 gcaaatgcta gcgacagcaa gcctgcacag gacaagaagg atgcaaaaca aggaacggaa
      421 gatagtaagg attcagataa gatgactgaa acaaactcag ttccggcagg agtgattgtg
      481 gtcagtctac ttgccctcct aggcgtgatt gccttctggc tgattcgccg taagaaagag
      541 tcagaaatcc agcaattaag cacggaattg atcaaggttc taggacagct agatgcagaa
      601 aaagcggata aaaaagtcct tgccaaagcc caaaaccttc tccaagaaac ccttgatttc
      661 gtgaaagaag aaaatggctc agcagagaca gaaactaaac tagtagagga gcttaaagca
      721 atccttgaca aactcaagta aaacatgatt tatgaaatgt cagacctaaa agacattttc
      781 gttcacaaaa gcagtgacga gtaatccttg tcgttgcttt ttggctattt tgggggataa
      841 gatccgcctt gactagcaaa agaaaagatt ggtccaatag tgcccgatac gattttgcgt
      901 agcagaaagc ttacaaataa aattgaatat agtaaaatag gattagaatt attaagaaag
      961 ttggttcttt attggaaacg atagtatttt taatctctgt ttttctagca ggtgttttat
     1021 cctttttttc tccttgtatt tttcctcttc tgccagtcta tgctgggatt ttattggatg
     1081 atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg tcaggcttga
     1141 ttcgaacact ttgctttatc gctggtatct ctctcatttt ctttattcta ggttttggtg
     1201 ctggttactt tggtcatatt ctctatgcaa attggtttcg atatgtcatg ggagctatta
     1261 ttatcatttt gggtcttcac cagatggaaa tttttcattt gaagaaatta gaagttcaaa
     1321 aaagttttac ctttaaaaaa tcagattcta atcgttattg gtcagctttt ttacttggta
     1381 ttacctttag ctttggttgg acaccttgta ttggtccagt tttaagttct gttttagcac
     1441 ttgcggcttc tggaggcaat ggtgcttggc aaggagcgat ttatactctc atttacactc
     1501 tgggcatggc ccttcctttc ttggtcttgg cactagcttc aggtctagtc atgccatatt
     1561 ttagtaaaat caagcgtcat atgatgctac taaagaaaat tggtggtttc ctcattgttt
     1621 taatgggaat tttgttacta ttaggacaag taaatgttct agctggaatt tttgaataaa
     1681 ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt agttgtattg
     1741 atggcctgtg gtgaggaaga aactaaaaag actcaagcag cacaacagcc aaaacaacaa
     1801 acgactgtac aacaaattgc tgttggaaaa gatgctccag acttcacatt gcaatccatg
     1861 gatggcaaag aagttaagtt atctgatttt aagggtaaaa aggtttactt gaagttttgg
     1921 gcttcatggt gtggtccatg caagaaaagt atgccagagt tgatggaact agcggcgaaa
     1981 ccagatcgtg atttcgaaat tcttactgtc attgcaccag gaattcaagg tgaaaaaact
     2041 gttgagcaat tcccacaatg gttccaggaa caaggatata aggatatccc agttctttat
     2101 gataccaaag caaccacctt ccaagcttat caaattcgaa gcattcctac agaatattta
     2161 attgatagcc aaggaaagat tggaaagatt caatttggtg ctatcagtaa tgcggatgca
     2221 gaagcagcat ttaaagaaat gaactagaat cgataaaatc tgattacaag atgtagtcag
     2281 atttttttag aaaaacattt tataaatatt cacatatctc ctatatttat ggtaaaatag
     2341 aattatcagt ttattttgga gtcaaagatg aatatattta gaacaaagaa tgttagttta
     2401 gataaaacag agatgcatag gcatttgaag ttatgggatt tgattttgct gggtatcgga
     2461 gccatggtag ggacaggcgt ctttacaatc acaggtactg cagctgcaac acttgctggc
     2521 ccagccctag tgatttcaat cgttatttct gccttgtgtg tgggattatc agccctcttt
     2581 tttgcagaat ttgcctcgcg agtacccgct acaggaggtg cctatagtta cctctatgct
     2641 atcttaggag aattcccttc ctggttggct ggttggttaa ccatgatgga gttcatgaca
     2701 gccatatcag gcgtagcttc gggttgggca gcttatttta aaggcttgct ctctcaatac
     2761 gggatagcct ttcctcaggc tttaaatggt acctttaatc ctcaagcagg gacatttgtt
     2821 gatttattgc ctattcttgt cttggtcttg gtgacttcgc ttgttttact aaatgccaag
     2881 gcagccttac gctttaattc gattctagtt attttgaaat tttccgcttt agctctcttt
     2941 gttttagtag gaatttggaa tattaagttt gataattgga gcaattttgc tccttacggt
     3001 tttggacaaa tctatggagc tagcactggt attatggctg gtgcgtcctt gatgttcttc
     3061 ggttttttgg gatttgaatc catctctatg gctgtagatg aagtcaagac tcctcaaaaa
     3121 aatattccta gagggattgt cttatcgctt tctatcgtaa ccattctcta tgccttggtg
     3181 acccttgttt tgactggtgt ggttcactat agtcatttaa atgtcgatga tgccgttgcc
     3241 tttgcccttc gtagtgttgg gattagttgg gcagccaact atgtgtcatt agtggctatc
     3301 ttgaccttga ttacagtttg tatttcgatg acctatgccc tatcgcgtat gatttacagt
     3361 ttagcacgtg acggcttagt gcctgctgcc tttaaggaac tgacgaagac tagcaagata
     3421 ccaaagaatg ctactatctt gacaggtcta gcttcagcag tagcagcagg aatgttccca
     3481 ctagccagta ttgcagcctt tttaaatatt tgtaccttag cctacttgat catgctggct
     3541 tatggtctga ttcgcttacg gaaagaaaaa ggaatgccca aagctgggga atttaaaaca
     3601 ccattggtac ctctgttacc aattttatcg atcatcattt gtttatcctt catgttacag
     3661 tataatatga acacctggat tgctttccta gttgcgttgt tagtaggaag tattatttac
     3721 ttcacttatg gctataagca ttctaccata gaagaataaa gtgaaagtct attgagttgt
     3781 tgacactcag tagattttta tatgtgaaag aaatttcaaa ttttttctaa aaatatcttg
     3841 acagattaaa ttttcaggag tagaatattt actagttaat taaatggtta aggagttgtt
     3901 catgaagaaa caaaatttat ttttagtcct gttaagtgtc tttcttttgt gtttgggggc
     3961 ttgtggtcaa aaggaaagtc agacaggaaa ggggatgaaa attgtgacca gtttttatcc
     4021 tatctacgct atggttaagg aagtatctgg tgacttgaat gatgttcgga tgattcagtc
     4081 aagtagtggg attcactcct ttgaaccttc ggcaaatgat atcgcagcca tctatgatgc
     4141 agatgtcttt gtttaccatt ctcatacact cgaatcttgg gcaggaagtc tggatccaaa
     4201 tctaaaaaaa tccaaagtga aggtcttaga ggcttctgag ggaatgacct tggaacgtgt
     4261 ccctggacta gaggatgtgg aagcagggga tggagttgat gaaaaaacgc tctatgaccc
     4321 tcacacatgg ctagatcctg aaaaagctgg agaagaagcc caaattatcg ctgataaact
     4381 ttcagaggtg gatagtgagc ataaagagac ttatcaaaaa aatgcgcaag cctttatcaa
     4441 aaaagctcag gaattgacta agaaattcca accaaaattt gaaaaagcga ctcagaaaac
     4501 atttgtaaca caacatacag ccttttctta tctagcgaag agatttgggc ttaatcaact
     4561 tggtattgca ggtatctctc ctgaacaaga accaagtcca cgacaactaa cagaaattca
     4621 ggaatttgtt aagacctata aggttaaaac gatttttaca gaaagtaacg cttcttcaaa
     4681 agtagctgaa actcttgtca aatcaacagg tgtgggtctt aaaactctga atcctttaga
     4741 gtcagaccca caaaatgaca agacctattt agaaaatctt gaagaaaata tgagtattct
     4801 agcagaagaa ttaaagtgag gaaagaatga aaatcaataa aaaatatcta gcaggttcag
     4861 tggcagtcct tgccctaagt gtttgttcct atgaacttgg tcgtcaccaa gctggtcagg
     4921 ttaagaaaga gtctaatcga gtttcttata tagatggtga tcaggctggt caaaaggcag
     4981 aaaatttgac accagatgaa gtcagtaaga gagaggggat caacgccgaa caaattgtta
     5041 tcaagattac ggatcaaggt tatgtgacct ctcatggaga ccattatcat tactataatg
     5101 gcaaggttcc ttatgatgcc atcatcagtg aagaacttct catgaaagat ccgaattatc
     5161 agttgaagga ttcagacatt gtcaatgaaa tcaagggtgg ctatgtgatt aaggtagacg
     5221 gaaaatacta tgtttacctt aaagatgcag cccatgcgga caatattcgg acaaaagaag
     5281 agattaaacg tcagaagcag gaacgcagtc ataatcacaa ctcaagagca gataatgctg
     5341 ttgctgcagc cagagcccaa ggacgttata caacggatga tgggtatatc ttcaatgcat
     5401 ctgatatcat tgaggacacg ggtgatgctt atattgtccc tcacggcaat cacttccact
     5461 atattcctaa gagtgatttg tctgccagtg aattagctgc tgcccaagcc tattggaatg
     5521 ggaagcaggg atctcgtcct tcttcaagtt ctagtcataa tgcaaatcca gctcaaccaa
     5581 gattgtcaga gaaccacaat ctgactgtca ctccaactta tcatcaaaat caaggggaaa
     5641 acatttcaag ccttttacgt gaattgtatg ctaaaccctt atcagaacgc catgtagaat
     5701 ctgatggcct agtatttgat ccggcacaaa taacatatcg tacagtcaat ggtgttgctg
     5761 taccgcacgg agaccattat cactttattc cttattcaca actgtcacct ttggaagaaa
     5821 aattggctcg tatgattgct gttaagggac aaaatggtgc agtcttgcca ggaatgcatt
     5881 atttgaaacc agcaccaaaa ccgcaagttc aaccatcaac tgagaaaaaa caaactgatt
     5941 ttgcagtaga acaggttgtt agaaaagttg gagaaggcta tgttgtggaa attgctggtg
     6001 tcagtcacta tgtttttgct aaagacttgg ctaaggataa gattgatgct attgaaaatc
     6061 tcttgtctaa gaaaactcaa gagacacatg ctctagttgc taaaaaagaa aatgtcgctc
     6121 ctcgtgacca agaattttat gataaagctt acaatctgtt aacccaggct cataaagtct
     6181 tgtctgaaaa taaaggtcgt acttctgatt tccaagcctt agacaaatta gcagaacgct
     6241 tgaataatga atcttctaat aaagtaaaat tggtagatga tttattggca ttcctagcac
     6301 caattaccca cccagaacgc cttgg
//
LOCUS       DAVBCG010000108         6222 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBCG010000108 DAVBCG010000000
VERSION     DAVBCG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.108"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            31..1797
                     /gene="patB"
                     /locus_tag="VQ495_001677"
     CDS             31..1797
                     /gene="patB"
                     /locus_tag="VQ495_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2531607.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            1936..2625
                     /locus_tag="VQ495_001678"
     CDS             1936..2625
                     /locus_tag="VQ495_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2531608.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            2629..2808
                     /locus_tag="VQ495_001679"
     CDS             2629..2808
                     /locus_tag="VQ495_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531609.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            2795..4144
                     /locus_tag="VQ495_001680"
     CDS             2795..4144
                     /locus_tag="VQ495_001680"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2531610.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4268..5728
                     /locus_tag="VQ495_001681"
     CDS             4268..5728
                     /locus_tag="VQ495_001681"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2531611.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            5742..6176
                     /locus_tag="VQ495_001682"
     CDS             5742..6176
                     /locus_tag="VQ495_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2531612.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         1951 a         1189 c         1346 g         1736 t
ORIGIN      
        1 tgcgctaaat ataatataag gaggagtaaa atgaagacag ttcaattttt ttggcattat
       61 tttaaggtct acaagttctc atttgtagtt gtcatcctga tgattgttct ggcgactttt
      121 gcccaagccc tctttccagt cttttctgga caagcggtga cgcagctagc caatttagtt
      181 caagcttatc aaaatggcaa tccagaactt gtatggcaaa gcctatcagg aatcatggtc
      241 aatcttggcc tgctggtttt ggttctattt atctctagtg taatatacat gtgtctcatg
      301 acgcacgtga ttgcagaatc gaccaacgag atgcgcaaag gcctctttgg taagcttgct
      361 cagttgacgg tttctttctt tgaccgtcga caagatggcg atatcttgtc tcattttacc
      421 agtgatttgg ataatatcct ccaagccttt aacgaaagct tgattcaggt catgagcaat
      481 attgttttat acattggtct gattcttgtc atgttttcga gaaatgtgac gctggctctc
      541 atcaccattg ccagcacccc attggctttc cttatgctga ttttcatcgt gaaaatggca
      601 cgcaaataca ccaacctcca gcagaaagag gtagggaagc tcaacgccta tatggatgag
      661 agcatctcag gccaaaaagc cgtgattgtg caaggaattc aagaggatat gatggcagga
      721 tttcttgaac aaaatgagcg cgtgcgcaag gcaaccttta aaggaagaat gttctcagga
      781 attcttttcc ctgtcatgaa tgggatgagc ctgattaata cagccatcgt catctttgct
      841 ggttcggctg tacttttgaa tgataagtct attgaaacaa gtacagccct aggtttgatt
      901 gttatgtttg cacaattttc acagcagtac taccagccta ttatccaagt tgcagcgagt
      961 tggggaagcc ttcagttggc ctttactgga gctgaacgaa ttcaggaaat gtttgatgca
     1021 gaggaggaaa tccgacctga aaaggctcca accttcacta agttgcaaga aagtgttgaa
     1081 atcagtcata tcgatttttc atacttgcct gataaaccta ttttgaaaga tgtcagcatt
     1141 tctgccccta aaggccagat gacagcagtt gttgggccga caggttcagg aaaaacgact
     1201 attatgaacc tcatcaatcg cttttatgat gttgatgctg gtggtattta ttttgatggt
     1261 aaagacattc gtggctatga cttagatagt cttagaagca aggtgggaat tgtattgcaa
     1321 gattcggtct tgtttagcgg aacgattcga gacaatatcc gatttggtgt gccagatgct
     1381 agtcaggaaa tggttgaggt agcagcaaaa gcaacccaca ttcacgacta tatcgaaagt
     1441 ttgcctgata agtacgatac tcttattgat gatgaccaga gcatcttttc aacagggcag
     1501 aagcaattga tttcaatcgc tcgaaccctg atgacagatc cagaagttct cattctcgat
     1561 gaagcaactt caaacgtaga tacggtgaca gaaagcaaga ttcagcatgc catggaggtg
     1621 gttgtagcag gtagaactag tttcgtcatt gcccaccgct tgaaaaccat tctcaatgca
     1681 gatcagatta ttgtccttaa agatggagaa gtcattgaac gtggtaacca ccatgaactt
     1741 ttgaagctag gtggctttta ttcagaactc tatcacaatc aatttgtttt cgaataagaa
     1801 agaagttgtc ctatgtgggc agctttttct tgtccataaa aaatgtttat cacagcctta
     1861 aaaaaaacat attagacgaa agtcattttg agtgatatga taggactatc gttagcattc
     1921 gaaaggagag gcatcatggc tagaacggtt gtaggagttg ctgcaaatct atgtcccgta
     1981 gacgcagaag gcaaaatcat tcattcatct gtatcttgta gattcgcaga gatcattcgt
     2041 caagtcggtg gtctcccttt agtcattcct gttggtgatg agtcagttgt acgtgattat
     2101 gtggaaatga ttgacaaact cattttgaca ggaggccaaa atgttcatcc tcagttttat
     2161 ggagagaaaa agaccgtcga gagcgatgat tacaatctgg tccgtgacga atttgaattg
     2221 gcactcttga aggaagcgct tcgtcagaat aaaccaatta tggcaatctg tcgcggtgtc
     2281 caacttgtca atgttgcctt tggtggaacc ctcaatcaag aaatcgaagg tcactggcaa
     2341 ggactacctt tcgggacatc tcactctatt gagacagtag aaggaagcgt ggtggctaag
     2401 ctatttggga aagaaagtca ggtcaactca gtccatcgtc aaagcattaa agatttggca
     2461 cctaatttcc gtgtaactgc tattgattca agagaccaaa ctattgaagc gattgagtct
     2521 atcgacgagc accgcattat cggtttacag tggcatccag agtttctggt taatgaagaa
     2581 gatggcaatt tagaattatt tgagtattta ttggatgaac tgtaacaatt ggaacatctg
     2641 gtcgttcttt cttttatttt ttcaaaattt ttagaatgtg gtattttacg caaacgtttg
     2701 aattctgata aaaattggag aaattcgaca aaaaaacttg aaaaaaacga aggtaagcgt
     2761 tatgatagaa aagaagaaat attggaggaa gaacatgtca catattaaat ttgattattc
     2821 aaaagtttta gacaaatttg ttgcaccaca tgaagtggaa tacatgcaat cacaagtaac
     2881 agcagcagat gaattgatcc gtaaaggaac tggtgctggt agcgacttct tgggatggtt
     2941 ggaccttcct gaaaaatacg accgcgaaga atttgaccgc atcttgaaag ctgctgagca
     3001 aatcaagtca gacagcgatg ttttggttgt gatcggtatt ggtggatctt accttggtgc
     3061 aaaagcagca atcgacttct tgaatcacca ttttgctaac ttgcaaacaa aagaagaacg
     3121 caaagctcca caaatccttt acgcaggaaa ctcaatctca tctacttacc ttgctgactt
     3181 ggtagagtat gttgcagaca aagacttctc agtaaacgta atttctaaat caggtacaac
     3241 aactgaacca gcgattgctt tccgtgtctt taaagaactc ttggttaaga aatacggtca
     3301 agaagaagct aacaaacgta tctatgcaac aactgaccgc caaaagggtg ctgttaaggt
     3361 tgaagcagac gctaacggtt ggggaacatt tgttgttcca gatgatatcg gtggacgctt
     3421 ctcagtattg acagccgttg gtttgcttcc aatcgcagca tcaggagctg acataaaagc
     3481 tcttatggaa ggtgcgaatg cagctcgcaa agactacact tcagacaaaa tctctgaaaa
     3541 cgaagcttac caatacgcag ctgttcgtaa catcctttat cgtaaaggct atgcaactga
     3601 gatcttggta aactatgagc catcacttca atacttctca gaatggtgga aacaattggc
     3661 tggtgaatca gaaggaaaag accaaaaagg tatctaccca acttcagcca acttctcaac
     3721 tgacttgcac tcacttggtc aatttatcca agaaggaact cgtatcatgt ttgaaacagt
     3781 tgtccgtgtt gacaaacctc gtaaaaacgt gcttattcct actttggaag aagaccttga
     3841 cggacttggt taccttcaag gaaaagacgt tgactttgta aacaaaaaag caactgacgg
     3901 tgttcttctt gcccacacag atggtgatgt accaaacatg tatgtgactc ttccagagca
     3961 agacgctttc actcttggtt acactatcta cttcttcgaa ttggcaattg ccctttcagg
     4021 ttacttgaat gctatcaacc catttgacca accaggtgtt gaagcttata aacgtaacat
     4081 gtttgccctt cttggaaaac caggatttga agaattgagc aaagaactta acgcacgtct
     4141 ataatagaag aaaagagtgg tttgcccact ctttttactc tctttatcca tagaaattgg
     4201 actcagccaa gacttgtgat ataatataga aagcaaaaag gcagacgcct agataatagg
     4261 agaaactatg tcaaaagata tccgcgtacg ttacgcacca agtccaacag gactactaca
     4321 catcggaaat gctcgtacag cattgtttaa ttacttgtat gcgcgccatc atggtggaac
     4381 atttctcatc cgtatcgaag atactgaccg taaacgccat gtcgaggatg gtgaacgttc
     4441 acaacttgaa aaccttcgct ggttaggcat ggattgggat gaaagtccag aatcacatga
     4501 gaattatcgc cagtctgagc gtttggactt gtatcaaaaa tacatcgacc aactattagc
     4561 tgaaggaaaa gcctacaaat cttacgttac agaagaagag ttggcagctg aacgtgaacg
     4621 tcaagaagta gctggcgaaa caccacgcta catcaatgaa taccttggta tgagtgaaga
     4681 agaaaaagca gcttacatcg cagaacgtga agcagcaggg atcatcccaa ctgttcgttt
     4741 ggctgtcaat gagtcaggta tctacaagtg gcatgatatg gtcaaaggcg atatcgaatt
     4801 tgaaggtggc aatatcggtg gtgactgggt tatccaaaag aaagacggtt acccaactta
     4861 caactttgcc gttgttatcg atgaccacga tatgcaaatc tctcatgtta tccgtggaga
     4921 tgaccatatt gctaatacac caaaacagct tatggtctat gaagctcttg gttgggaagc
     4981 tccagagttc ggtcacatga ccttgattat caactctgag actggtaaga agttatctaa
     5041 acgtgatact aatacacttc aatttatcga agattaccga aagaaaggtt accttccaga
     5101 agcagtcttt aattttattg ctcttcttgg ttggaaccca ggtggtgaag atgagatttt
     5161 ctctcgtgaa gaactcatta aactttttga tgaaaaccgc ctcagcaagt caccagcagc
     5221 ctttgatcag aaaaaactag actggatgag caatgattat atcaagaatg cagaccttga
     5281 aaccatcttt gaaatggcaa aaccattctt agaggaagca ggccgtttga ctgacaaggc
     5341 tgaaaaatta gttgagctct ataaaccaca aatgaaatca gtagatgaga ttatcccatt
     5401 gacagatctt ttcttctcag atttcccaga attgacagaa gcagagcgcg aagtcatgac
     5461 gggtgaaaca gttccaacag ttcttgaagc attcaaagca aaacttgaag cgatgacaga
     5521 tgataaattt gtgacagaaa atatcttccc acaaattaaa gcagttcaaa aagaaacagg
     5581 tattaaaggg aaaaatcttt tcatgcctat tcgtatcgca gtttcaggcg aaatgcatgg
     5641 gccagaatta ccagatacaa ttttcttgct tggacgtgaa aaatcaattc agcatatcga
     5701 aaacatgcta aaagaaatct ctaaataaag aggatacaat aatggacatc tgggaaaaga
     5761 tgtacgaaga agcacagaaa ctatacaatc cacatgaagt atcagacttt gtttatgcca
     5821 atcatgttgt agctgcagta gaagcagaag atggacaaat atttacagga ttctgtatgg
     5881 agggaacctg tggtgttttc catctctgcg cagaacgggc ggccctcttc aatacgtacc
     5941 aattttcagg acaaactaag gttaagaaag tattagcctt tcgagacaaa ccaccttatg
     6001 gtggaagttc agccatgcct tgcggtgctt gtagagaatt ccttttagag cttaatgctg
     6061 aaaataaaga tgcagaattc atgatggact ataatataag aaaaacagtt aaagtcgcag
     6121 aactaatccc ttattggtgg ggagaagaac gtgcttctaa gtttaatgag cgatagaaga
     6181 gtcagtataa ttctggcttt ttttactata agttgaaaaa at
//
LOCUS       DAVBCG010000109         6046 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBCG010000109 DAVBCG010000000
VERSION     DAVBCG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.109"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            219..1988
                     /locus_tag="VQ495_001683"
     CDS             219..1988
                     /locus_tag="VQ495_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HET2531613.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGAFPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            2012..3967
                     /locus_tag="VQ495_001684"
     CDS             2012..3967
                     /locus_tag="VQ495_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2531614.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTIKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3969..4406
                     /locus_tag="VQ495_001685"
     CDS             3969..4406
                     /locus_tag="VQ495_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2531615.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4468..5604
                     /locus_tag="VQ495_001686"
     CDS             4468..5604
                     /locus_tag="VQ495_001686"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET2531616.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIDQIVEYI"
BASE COUNT         1847 a         1015 c         1232 g         1952 t
ORIGIN      
        1 ttcatgataa aaacacccca aaagttagat tttttctgtc taacttttgg ggtgcagttc
       61 atcatttcaa catcctatac tatgcgtttt ataattttta agcctttttg cccagcctcg
      121 tcaaaagtaa tgttttgaca caaaatctgt gacaaaactt tagttttaaa ggtttttaac
      181 tttgtatata ctagttttaa gaaaaggagg atgatctaat ggaagaaaaa gtatcattga
      241 aagtcagggt tcaaaaacta gggacatcgc tttcaaatat ggttatgccc aatattggag
      301 catttattgc ttggggagta ttgactgccc tctttatcgc tgatggctat ctgccaaatg
      361 aacagttagc tactgttgtt ggtcctatgt taacgtattt attgccaatc ctgattggtt
      421 acacaggtgg atatatgatc catggccaac gtggtgccgt tgtaggagct attgctactg
      481 ttggtgcaat cacaggttct agtgttccta tgtttatcgg agctatggta atgggcccac
      541 tgggaggatg gactatcaag aaatttgatg agaagttcca ggaaaaaatt cgtcccggat
      601 ttgaaatgtt agttaataac ttctcagctg gtctcgttgg ttttgcatta ttgcttttgg
      661 ctttctacgc aatcggtcca gtcgtatcga ctcttactgg agctgttggg aatggtgttg
      721 aggctattgt caatgctcgc ctccttccta tggctaatat tatcatcgaa ccggctaaag
      781 tccttttcct caataatgcc ctcaatcatg gcatttttac tcctctggga gtagaacagg
      841 tagctcaagc tggtaagtca attctcttcc tattggaagc taatcctgga ccaggcctgg
      901 gaattctatt agcttatgct gtattcggta aaggttctgc taaatcttct tcttgggggg
      961 caatggttat tcatttcttc ggagggattc atgaaattta ctttccttat gttatgatga
     1021 agcctactct atttttagct gctatggcag gaggtatctc tggaactttt acttttcaac
     1081 tcttagacgc tggtcttaaa tctccagctt caccaggttc tattattgcg attatggcta
     1141 cggcgccaaa aggtgtttgg cctcatctaa atgttctttt aggtgtttta gtggcagcag
     1201 ttgtttcttt ccttgtagca gcccttattc ttcatgcaga caagtcaact gaggattcgc
     1261 tcgaagctgc tcaggcggct acccaagcag ctaaggctca gtctaaaggt cagttagtat
     1321 caacttctgt tgatgcagtt gtttcgacag actcagtgga aaaaatcatt ttcgcctgcg
     1381 atgctggtat gggaagctct gctatgggag ctagtattct tcgagataag gttaaaaaag
     1441 caggtctaga gattccagta tctaatcagg caatctcaaa tttgcttgat acaccaaaaa
     1501 cattaattgt tactcaggaa gaactgacac caagagctaa agacaagagt ccaagtgcta
     1561 ttcatgtttc tgttgataat ttcttagcgt cccctcgtta tgatgaaatt gtagcttcat
     1621 taacaggagc ttttccaata gcagaaattg aaggagatat accaacttca gcaccagtag
     1681 atagtcagga aattgacctt aaccatattg atgctgtagt agttgcttat ggtaaagcac
     1741 agggaactgc aactatgggc tgtgaaacga ttcgggctat ctttagaaac aagaatattc
     1801 gtattccagt ttctactgcc aaaatttcag aattaggtga atttaattct aaaaacataa
     1861 tgattgtaac aactatttct ttacaggcag aagtgcagca agcagcaccg aattctcaat
     1921 ttcttattgt ggatagttta gtaacaacac cagaatatga caaaatggct gctagaatgt
     1981 acaaatagaa ctagaggttt ctaaattacg aatgctatta accaaacgag aagaacaatt
     2041 attgaaggct ttcctacatg tagggaagct ttcaatgcaa gatatgactg aaatcttaca
     2101 ggtttcatct agaacaattt atcgaacttt atcagatttg acagatagca tggagcaata
     2161 tggaatcgaa ataacgaagc atgggaaata ctatattttg actggagagt tggatgattt
     2221 gccgacagaa cttgaagtgt tagttgagta tagtccccaa gaaagacaag agttgattac
     2281 ctatcgcctt ctgactgaga gcggttttgt caccaatgaa gcattgcaag agtgcacgaa
     2341 agtcagtaat gtaactatta ttcaggatat ttcagatatt gataagcgtc ttttagactt
     2401 tgatctgaaa attgaacgac aaaaaggtta tcggatttct ggtgattcag ttggtaagag
     2461 aagatttttg gctattttac tgacaaactg tatctcagta gcagattttt caaccggtaa
     2521 ttttgggagc tttgatattt tagaagcaga tagaactagg ctggccagtc agattgttaa
     2581 taagcaactg tcaggttttc cagatatgga tgctaggatg aagatgtttt ttgcgatctt
     2641 gttatctctt ataggtcagg agcaaaacat tgaaaattca cctaatacta gtaagcaggc
     2701 tttggaaatt tctcaaaaaa tttttcaaac ttactctaag cagactgcac aattttatag
     2761 tattcaggaa attatctatt ttgcgagcat cttggatgaa ttaatcatta aacgtcagga
     2821 caatccgctc tttacggaga aatttgatgg tgaatttttc tacaatattt caaatctgat
     2881 tgatacggtt tccatgtata ccaagattga cttttttaag gacaaggttt tattcaattt
     2941 tcttttccat catattcggc tcagtttagg cgtccctatc ctttttcagg gtgaaaattt
     3001 gccagaatct gtccagattt tagttgaaag gaataaattt ctttatacag tcatcagtct
     3061 tttagtgaat gatatttttc cgaaatatct tcatacagag tatgagtatg gcatgattgc
     3121 cctacatttt atctctagct taggccgtag tccagagatt tatccagtcc gtgttttgct
     3181 tttaacggat gaacgtcggg tcactagaga tttattagtc agtaaaatta agagtgttgc
     3241 tccttttgta gagttgatag atattcagtc tctagtagat taccacagta ttgatctcag
     3301 tcagtatgat tatattttat ctaccaagcc gctgactaat caggaaatcg atgtaatttc
     3361 tagttttcca accatcaaag aattgcttga attacaggaa cgacttcagt atgtacaggc
     3421 acatcgtaca attgtcgcgc gtgatgctat cgctccagag aaaagttatg acttgcaaga
     3481 ttatttaata tctagtagtc agcttttgag tcaattcgag ttggttcaat tggagaataa
     3541 tcaatcattt gagcacacgg tagaacaaat catccaatat cagaagaatg tgagtgacag
     3601 agattaccta acaagaaaat tgttatctca cttccagaat agtcctatgg ctattcctaa
     3661 tactggtctg gtgcttttac atagtcagtc tagcaaagta acaacaaata gttttactat
     3721 gtttgaactc aaactaccta tctccgcatt gtcaatgaaa cgagaggaag aagaggtcaa
     3781 aaggtgtctg ctaatgctaa tgtctaaaga agctagcgag gaagcgagag atttaatgac
     3841 agctattagt cagtcgatta ttgaaaatca tctttataca gagatttaca agacgggaaa
     3901 tcaatccatt atttatcaga tgctaaatac tatttttaac gaaaaaatta agaaattgga
     3961 gaactaatat gaaacttgaa aaacatttga ttaagcttaa taaacaattt tctaacaagg
     4021 aggaagctat ttgttattgt gggcaagttc tttatgaggg tggatatgtt aatgaagact
     4081 atattgaagc catgattgag cgagataaag agctatctgt ttacatgggt aactttatcg
     4141 ccataccgca tggaacagat gcagcaaaaa aggatgtcct caagtctggt attacagtcg
     4201 ttcaagtccc tagaggggtt gattttggga atgtatctaa ccctcaagtg gcaacggttc
     4261 tttttggtat tgctggtatt ggtaatgaac acttagaaat tattcagaaa atttctatct
     4321 tctgtgcaga tgtagataat gttcttaaac tagccgatgc tcagtcaaaa gaggaagtat
     4381 tgcgcttatt tgatgctgtt gaataattga atttcgtcat ttgccatcta gtatatatgt
     4441 ccctcaaata ggaaaaggag aaattgaatg aaacattctg ttcattttgg tgccggtaat
     4501 atcggtcgtg gttttatagg tgaaattcta tttaaaaatg gtttccatat tgattttgtg
     4561 gatgtcaata atcagataat tcatgctcta aatgaaaagg gcaagtatga aattgaaatt
     4621 gcacagaaag gacagtctcg tatagaagta actaatgtgg ctggcattaa tagcaaagaa
     4681 catcctgagc aagtcattga agcgattcaa aagacggata ttattactac tgcaatcgga
     4741 cctaatatac tcccttttat cgctgaactt ctagccaaag gaatcgaagc tcgccgagtt
     4801 gcagggaata cacaggtatt ggatgttatg gcctgtgaaa atatgattgg cgggtctcaa
     4861 tttctttatc aagaagtcaa gaaatattta agtccggaag gtttgacatt tgctgataac
     4921 tacataggtt ttccaaatgc tgcagtagac aggattgttc cagcacaaag tcacgaagat
     4981 tccctttttg ttgtggtcga gccctttaat gaatgggtcg tggaaaccaa gcgtcttaaa
     5041 aatccagatt tacgtctaaa agatgtgcat tatgaagaag atttagaacc ctttattgag
     5101 cgaaaacttt tttcagtcaa ttctggacat gcaacttcag cttacattgg tgcgcattat
     5161 ggtgccaaga caattttgga agctcttcaa aatcctaata tcaaatctcg gattgaatct
     5221 gtattagctg aaattcggag tctcttgatt gccaaatgga actttgataa aaaagaattg
     5281 gagaattatc acaaagtcat tatagaacgg tttgaaaatc ctttcatagt ggacgaggtt
     5341 agtcgcgtag ctcgtactcc aatccgaaaa ttaggctata atgaacgatt catccggccg
     5401 atacgtgaat tgaaagaact cagtttgtca tataaaaacc tacttaaaac agttggctat
     5461 gcctttgact atcgcgatgt aaatgatgaa gaaagtattc gattaggtga attgttggct
     5521 aaacaatcag tcaaagatgt tgttatacaa gttacaggtt tagacgacca agaattgatt
     5581 gatcaaattg tagagtatat ttaatatttt tcgaaaatct cttcaaatca ggttagcatc
     5641 gctttgtctt aggcatatgt tactgactcc gtcagtctta tctgcaacct caaaacactg
     5701 tttgagttat ctgcggtaat ctttctagct tgtctttgat ttttgttgtt atttataagg
     5761 taaaagaagc tggacaaaaa gtcttcaaaa tcggaaaaag gcagcctatc gggtgttcaa
     5821 aaatcttgat aggatgtcct ttattatgga aagccttatt ggattttctc ctcagattga
     5881 gtttttgatc agctttatga gataggtttt gctagagatg tagcccatca tgctattttt
     5941 atggacagtg ggaaaattgt tgaaaaaaat aatgcccatc aattctttag tcgtccaaga
     6001 gaagaacgaa ccaagcaatt ttggaacgaa ttctttcgaa tgcgat
//
LOCUS       DAVBCG010000110         6034 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBCG010000110 DAVBCG010000000
VERSION     DAVBCG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.110"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..634
                     /locus_tag="VQ495_001687"
     CDS             <1..634
                     /locus_tag="VQ495_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET2531617.1"
                     /translation="KHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSV
                     APRSIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAV
                     ILAEIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKAC
                     ARFSPAFKAYLKTKLEQGKHYNVAIIHLAKKLIRTLFYILKKSCHLTNKK"
     gene            complement(833..2722)
                     /locus_tag="VQ495_001688"
     CDS             complement(833..2722)
                     /locus_tag="VQ495_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2531618.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2860..4425
                     /locus_tag="VQ495_001689"
     CDS             2860..4425
                     /locus_tag="VQ495_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2531619.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIALPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            4425..5165
                     /locus_tag="VQ495_001690"
     CDS             4425..5165
                     /locus_tag="VQ495_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2531620.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(5274..5489)
                     /locus_tag="VQ495_001691"
     CDS             complement(5274..5489)
                     /locus_tag="VQ495_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531621.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         1878 a         1358 c         1070 g         1728 t
ORIGIN      
        1 aaagcattca gaatatacct accaactctt gacgcgctac cctaatcctc agaaaaggat
       61 tgaggcagga tttgataagc tgatagaaat taagcgattg accgcttcta aaattcagga
      121 tatcctctca gtcgcacctc gttctatcgg aacaacatct cctgctcgtg aattcgaaat
      181 catcgaaatc atcaaacatt acaagaggct cattgacaag gcggaaacat gtgtcaatga
      241 cttgatggct gagttcaact cagtcatcac gacggttact gggattgggg gtcgtttagg
      301 ggcggtcatt ttagccgaga ttcgaaatat tcatgccttt gataatcctg ctcaattaca
      361 agctttcgct ggactggatt cttctattta tcagtcaggt cagattgatt tagctggaag
      421 aatgatcaaa cggggttccc ctcatctgcg gtgggcactc atacaagctg ccaaagcatg
      481 cgctcgcttt tcacctgctt ttaaggccta tcttaagact aagttagaac aaggaaaaca
      541 ttacaatgta gccatcatcc accttgcaaa aaaacttatc cgaaccctgt tttatatcct
      601 aaaaaagagc tgccatttga cgaacaaaaa gtgatgtaat ttctgaaatt aacacaggat
      661 tacctttgaa aaaatggact ttttcaaggg ctttttgccg tgctctaaaa tcatcgccaa
      721 atcatcaaat tgaaataaga aatagtttag ataaaactat tgacttttct tatagtttgt
      781 ctttcttcta tcttccatta tatcatactt tttcagtcta tggtagctct ctttaaaagg
      841 ctccgatact gccgccacct ccgcctccag agaagccacc gccagaactt ccacttccag
      901 aagatacgga gtaggtactt gctgtatttg cgacactagc ataatggctc atttgcgcgc
      961 ttgaatgata aaacatactg tgccagccat aagctacata gagattgata tctggatttt
     1021 caacttgaat ctgatgaacc ttcatcaaat gactaacctt gtccgcatag ccaaataagg
     1081 ttgcatagac caagagacga ttccaaacaa caatactttc caattcagcc ttatccagtc
     1141 gtgcaatctc acgtaacata ttttcaaaac tggtccagag atagtagacc tcagctcccg
     1201 cttcatttag aacaccatca cgattatcta gtcgaagctt ccaataatag aaaacagaca
     1261 aaactaaccc tagaaaacca agtattggca aagggaggta aagatagcca tgaacgtcta
     1321 aactgtacaa gaacaaacca aatccgataa ataggggcaa gatagtcaag acacccatac
     1381 ccacttgcaa agtcttttcc aaaccagtta aaggacgata gtaatctggg agcccccaga
     1441 aggaaactcg ttttctcact ccttcttgca tctggttcaa tacctcttca aaagaagatt
     1501 tgagttgaag ccctcttgct tgaatccgtt tttcatcaga aactttggct ctacgataaa
     1561 gactatcaga taccttgtaa tccgcaaaca aattggaaag agtggcttct tttttgcctg
     1621 aaaaggctag atttagacag tctctctcaa atcttgacaa accatcttct ttcaccaatc
     1681 tcaagccaac tgcatctcct tctgaaataa tagagacatt cccacggtct atcacatcta
     1741 gcaaggtagc ttgaataagt tgatcaaagg tgaattttcc agctcccttg accaagggac
     1801 tcacttcctc caaggaggtc gagtagactg cttctgataa aaccataggc tctaattcca
     1861 ttggtggttc atagagacga tgatttttgg catatttgac tgaaggagtg gtctttcttc
     1921 tataaataaa atagaagcag acactcaata acaaggagat agaaagtatc gaagggagga
     1981 cccaagtaac gagttgttta ctctgatctt tttctctaac aatcgagtct tctatcttat
     2041 taaactcttc taaacgattc cctttcaagc cctgatccct agcgctagca aaatcggtcc
     2101 gagaccaata ggcatgcaac tcaactccac gcttagccgg aagattgtct aaacggatag
     2161 tataatcaag gttactcttt tcaatcgttc cctctctaaa aagtttccct gtatggaaaa
     2221 agagtttttc agcccccttg tctcccctta catgaaattc aaacttttca atagactctg
     2281 aactatctgt cagaggttgc caatttaatt cagcgatatc atcataaagg aaaagtaaat
     2341 tttttaagtt ccagacgagg tcaacttcaa ctatgtcgcc ctcctgacct ggattataga
     2401 ctctcacagt ataaccatcc gcttcttctg ttacttcgct agtcacatct gctagttctg
     2461 caccgttttt cgcagcctga acctttggat gagggtcaat gtcaaacccg ctaggcatct
     2521 taccagcacg tccaagtccc acgatttggc ccttaaagtc ctcctcaaac tggtaaacta
     2581 tcttctgtct aaactctgcc gtattatctg catgaatata caaatcccct tgataagagt
     2641 ttatcttgaa atcaatggca aaaacagaga gtggaagaag gcaaaacaag cctaacacca
     2701 gtaagaaaaa agtttttttc atcaactaag cccccttttt atctttctta tcattatagc
     2761 atcttttcgt aagtaaaggc ttacttatgt tgaaaaatta cgcatttttc gataaaatag
     2821 gagacagtta ttttttaata gatcagaata ggagaaatca tgagaaaaat atacttatct
     2881 attttcacaa gtctcttgct gatgctagga cttgtcaatg ttgctcaagc cgatgaatat
     2941 ttacgcatcg ggatggaagc agcatatgct ccctttaact ggacccagga tgatgatagc
     3001 aacggagctg tcaaaatcga tgggaccaat cagtatgcca acggatacga tgttcaaatc
     3061 gccaagaaaa tcgctaagga cttaggtaaa gaacctttgg ttgttaaaac caagtgggaa
     3121 ggtctagtcc ctgcccttac ttctggtaag attgacatga ttatcgcagg tatgagtcca
     3181 actgcagaac gcaaacaaga aattgccttt tcgagcagtt actacactag cgaaccagtt
     3241 ttgcttgtca aaaaagattc tgcctacgca agtgctaaat ctttggatga ctttaatggt
     3301 gcgaaaatca cttctcaaca aggtgtctac ctctacgact tgattgcaca aatcccaggt
     3361 gctaaaaaag aaacagccat gggagacttc gctcaaatgc gccaagctct tgaggctggt
     3421 gtcattgatg cttatgtttc tgaacgtcca gaagcactga ctgctgaagc tgcgaactct
     3481 aagttcaaga tgattcaagt agaacctggt ttcaaaactg gggaagaaga tacagctatc
     3541 gctatcgggc ttcgtaaaaa tgacaatcgt attagccaaa tcaatgccag cattgaaacc
     3601 atttcaaaag atgaccaagt tgccttgatg gatcgtatga tcaaggaaca acctgccgaa
     3661 gctacaacaa ctgaagagac tagcagtagt ttctttagcc aagtcgctaa aattctttct
     3721 gaaaactggc aacaactctt gcgtggtgct ggtatcactc ttttaatctc tatcgtcgga
     3781 accatcatag gtctcattat tggacttgcc attggtgtct tccgtactgc tcctctctct
     3841 gaaaacaaag ccatttatgg ccttcaaaaa ctagtcggct gggttctcaa tgtctacatt
     3901 gaaattttcc gtggtacgcc aatgattgtt caatcgatgg ttatctacta tggaactgcc
     3961 caagctttcg ggatcaacct tgaccgtaca ctggctgcta tcttcatcgt ttcaatcaat
     4021 accggtgcct acatgactga aatcgtccgt ggtggtatcc tagcagttga caagggacaa
     4081 tttgaagctg cgactgctct tggtatgacc cataaccaga ccatgcgtaa gattgcccta
     4141 cctcaggtag tccgtaacat cctacctgca actggtaatg aatttgtcat caatatcaaa
     4201 gatacatctg tattgaacgt tatctctgtt gtcgaacttt atttctcagg aaataccgtg
     4261 gcaacacaaa cctatcaata cttccagaca tttacaatca tcgccgtgat ttactttgtc
     4321 ctcaccttca ccgtaacacg tatcctacga tttatcgagc gccgaatgga catggatacc
     4381 tatactacag gtgctaacca aatgcaaacg gaggatttga aataatgaca caagcaatcc
     4441 ttgaaattaa acacctcaaa aaatcctatg gacaaaacga agtgctaaaa gacatttcac
     4501 tcactgtcca caagggagag gtcatctcta tcatcggaag ctctggaagc ggaaaatcga
     4561 ccttcctacg ctccattaac ctacttgaaa caccaactga tggacaaatc ctttatcatg
     4621 gacaaaacgt cctcgaaaaa ggctatgacc tcacgcaata ccgtgaaaag ttggggatgg
     4681 ttttccaatc ctttaacctc tttgaaaatc tcaatgttct tgaaaacaca atcgtcgctc
     4741 agacaactgt cctaaaacgc gaacgcacag aagctgaaaa gattgccaaa gaaaacctgg
     4801 aaaaggtcgg catgggagaa cgctactggc aagccaaacc aaaacaactc tcaggtggtc
     4861 aaaaacaacg tgtggccatc gctcgtgccc tctccatgaa tccggacgct attctctttg
     4921 atgaaccaac atcagctctc gatccagaaa tggttggaga agtcctcaaa atcatgcagg
     4981 acctggctca ggaaggcttg accatgattg tcgtaaccca tgaaatggaa tttgcccgtg
     5041 atgtctctca ccgtgttatc tttatggata agggcgtgat cgctgaagaa ggtaaaccag
     5101 aagacctctt caccaatcct aaagaagacc gaacaaaaga gttccttcaa cgctatctca
     5161 aataaaaaga aaaggctgca tcaaccgtgc agtctttttg ctgtcctcaa aatgaaaagg
     5221 cagattctct atctctacta cttgaaggtc aagaatccgc cgttatccaa agattaatct
     5281 tcaaattttt cataattttt ttcatagaaa tcaattaagc ctagggctgt ttcaaatgaa
     5341 atatttttta cttttgcacg accctgcgcc agagcaatga tagacatttc acgcgcattc
     5401 gtttctttag agatacggta gcctgtgatt ttcttatcac gaacccagcc aacaactgat
     5461 tctacttttt caaagtttga cttagccatg tttttctcct attttcttct ttataatatt
     5521 taattgtata cgtttttact acttttgtca ataaaaaaaa catatattca acgaaaataa
     5581 ttagttcaga attttcttac ttccttttta aatccgataa tatataggtt ttgctagcct
     5641 ctacaccata aatatttgct aataaaacta ctgtattatt accctcttaa ggtgcgaaat
     5701 tattcaagtt cttaaaaaat gcttctctat ttccgtaaga tagcttattt cttcgattta
     5761 agaactgaag atgatagttc taacctttag aaatccaagc cttcactagc tcaaaaactc
     5821 ctatctttct tcctcaataa atcatctgag agataggtat tccttatatc cttcaaattc
     5881 atcactcctt tggatattga ggtgctagcc tagaagcaga cacccgatta tctaaaccat
     5941 ccttaataga gatgacaaga cagataagct cagttaatta cttatatatc tttttaatat
     6001 ggtatatttg tcaacaaaaa gaaatcaaat cata
//
LOCUS       DAVBCG010000111         5995 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBCG010000111 DAVBCG010000000
VERSION     DAVBCG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.111"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            520..1539
                     /locus_tag="VQ495_001692"
     CDS             520..1539
                     /locus_tag="VQ495_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2531622.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
     gene            1552..2655
                     /locus_tag="VQ495_001693"
     CDS             1552..2655
                     /locus_tag="VQ495_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531623.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLTNIKTDYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIIDLEDDKK
                     RLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSVKQRIVS
                     SISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAKSGLLYN
                     SLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIFSMLQVS
                     SVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            2684..3592
                     /locus_tag="VQ495_001694"
     CDS             2684..3592
                     /locus_tag="VQ495_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531624.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            3594..5492
                     /locus_tag="VQ495_001695"
     CDS             3594..5492
                     /locus_tag="VQ495_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2531625.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            5516..>5995
                     /locus_tag="VQ495_001696"
     CDS             5516..>5995
                     /locus_tag="VQ495_001696"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2531626.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         2250 a          644 c         1042 g         2059 t
ORIGIN      
        1 aacgacatgc gttaaaagtt agttgaaccg ccgtatgccg aacagcacgt acggtggtgt
       61 gagaggggct agagattatc ccctactcga ttgtctatag ctataggggt aaagttatta
      121 gagaggttgc tagtttatag agacatcttg atttgtaaaa agttaaattt tagacaatta
      181 tcccctttaa aaaatctatt aaaccatagc tagttgaagt aaatttttat agaacataat
      241 tttatttatc aagacattgt agagttgagg caaattgaaa aagaaagttt aaaagttgat
      301 aataagtcaa taagaaagct attaattcta acgagattat ttgatagtaa tttgtttttt
      361 cagctactat tcggacaatg ttattataat aaacacagga gagaacacca gaaggtaggg
      421 acatcagaag aaatagttca ggatatagtt aacataatca tcaaagaagc tcctagactc
      481 gctaatgtta atttggaaac atattatcca gatgaatata tgtcaaatac attgaagcaa
      541 ttactacttt ttagagagga gttcataaaa aatattttga atgggattga tgtggatgaa
      601 acagtagtaa tagaaaatga ttttgtagaa gagttggaga actttttaaa agagaatgat
      661 ttgctagatt tttcaaatag catatttttt cagttatctg gagataaagt aatagtgaat
      721 aatatttatc caggatattt agcatatttt aataggttcc ttacatttta tccgaaaata
      781 ttcgctatta aagaagttgg agaatatatt gactttttaa attttgaaaa gagaattgcg
      841 gatatgtttt attgttgggg atttaatgca aattctagag tgttgacagc tgaaaaaata
      901 attgaaatcc caaatcacca aatcaatgct aagtacataa aaaaagttat aaaaatgagt
      961 gaactcttgt ttggtgttga tagtaataac agaattcggt tgcttaacaa tagaagggaa
     1021 ttgataaaaa ctattttcca aggttctgta attaagtcag caattcctgc tctagctgct
     1081 actttggata caatcagtaa cagtggatca tttcaaattt ctttatcaaa gcagataatt
     1141 aataatttag tattaagtgg taaagataaa gttacaattc ctaggataga gttagataat
     1201 gttgttcttt caagacagaa gattattgta tcagtttcgt atataaatga ttttttggat
     1261 ttgagatttc cagataaaat agaatacatt ttaaattttt tagaaggatc tggtttcaat
     1321 actaaagtaa tggtctactt tgggaaaatt gtagaagaag ataagaacta ttttgttaat
     1381 cttgaaaaac ctcagtattt tgattttacc agtatacttt tcaataagtt attctttaaa
     1441 gaattaaaca agtttgatta catggttatt gaagaactca ttccaaatct tgatgaagat
     1501 gtaatgatga cagagtatat aaaagagtat actgaatagg agcaatctat tatgatttta
     1561 agtaaagtac gttgttacat atataattct aaaattagag gtaaagttct gtatgaatta
     1621 tatgaattta taaatgaaaa taagcaattg atggacgcaa ttaattcaat taaagttgac
     1681 agtagtaatg gttttagtat tcaaatatta catttaggag aacagcaaac aactgtctta
     1741 ttaacagatt tttttactaa aactttagac ttgtttaagg aatttagggt tacaaataag
     1801 agtactcttg agcaggtgaa aaatcaaatt agcatactaa attcttctgg tccaaaaatg
     1861 gatttaacga atataaaaac agattactca atagtggaag aaaaattaga ttggcaaagt
     1921 aaattatcta aaatttatcc gaatgttgaa tttataaaaa ttgaaaaatt aaaattgatt
     1981 ttgttgtgta aatatagaaa tcaaattatt gacttagagg atgataaaaa aagattgtta
     2041 ctattgaaat tattatattt gacttcaaag caattttgct atgatgaatt taaaggagcg
     2101 aaatttggat gtatgtcatt tttttcacat tatcaaggtt atatgagcag attagattca
     2161 ctcgactcag ataaatttgt ttcggttaaa cagaggattg tttcctctat ttctaaatta
     2221 gttgagcaat attcagatca tattataaat ctggatagaa ctttagttga ttctctaagt
     2281 gatacccttg agaatcagct atttgatgat tgggattata tattaagcga aataactaca
     2341 tatagcgaat tattagcaaa atcaggattg ttatataatt cgctagacta taagattgat
     2401 aatgtaaaaa aagaagtggc caagctaagt gactttcacc aatctttatt taaaattgaa
     2461 ggattttcag atttttttga tagttggcaa tttcaaagat ataggtctct cgtgaaccaa
     2521 gtttatgaaa ttttttcaat gttacaagta agtagtgtag aaagaactgt gttgtgcgga
     2581 gctctaagta ctattgtctt tagtaagtca aattttaact atcaagcttt ttttgagtat
     2641 ctaaaagata cgtagaattc gattttaaag gaggctgaaa tatatggatt atgtagatgt
     2701 aatattggat gagtatggga gtgtttttct gataaaggat cacataaaac taaaaattaa
     2761 taatctagaa ctatatcggt attttagtag ctttggaatg agtcatcttc aatttagaaa
     2821 agaagagctt ccaggaaaat taaagcagtt tgattttgaa aaaaataaaa gtgacagtta
     2881 tttttctaag aaaagtagtc tgattgattt tattaagaga aattattttt atgatattaa
     2941 gttatattct gataatccga aaaatccgat agagtcttgg aaaaataaga cagaaaaaat
     3001 taagattgct aaaagtacag aaattctttg gagtgaagta gcaaattttt cgtcaatttc
     3061 taaatctcct tactattgct tgttatataa tttaaagaat actgaaaatt acatattgat
     3121 aatcagtaag acatattttt cagccatcca aaaaatggtt tcgtataatc taaataatta
     3181 ttttgaatta gaagatgttt gttgtaaaag catagtgagc aagcgagaat tgatagaaaa
     3241 ttcaattaat attaacaaaa gcaaaatgaa actgctaaat gtttgcgaag gtagtaaagg
     3301 ttacacaaga agagtattta agtacaataa tatttttgtg ggacaattgg aatttcactc
     3361 taacataatc ttttcagaaa ttgctttaaa tgaattaagt attgttaagc ttttagattc
     3421 tgttgaaagt gttttatctg agcaactatt tggaaaatta aattgggata atttatttgt
     3481 tgttttatca gatgatttta atgattatct ggatataaat gaatctcaaa aatactactc
     3541 aaatggttct ttgagaggac tggagcttgg aatctttatg ggaggggttt aaaatgagaa
     3601 taattgaaat tgttgatgtt gaggaccaac ttttacaact tttaagtgca cataccaata
     3661 ttgatattag agcacaagag ttatcaacac ttgacccaga ttcaaaagtt gatttgatca
     3721 tatacttgaa atctcgacgt gatattgaaa acataaataa tattattatt aaatcaccta
     3781 acaggaatat cattgttata ataagtgctt ttgatattat atatgcattg tattttccaa
     3841 aatcaacatt tagaccttgt ttgaactgtc tattattaaa acttaaagat aacaaccctg
     3901 agcataatct attattattc ctgaaatacg gaacattacc tagtaaacat tttaatcttg
     3961 ataataggtt aaaatcaaat ttaatactat ttaatgagaa gttagaccct aaggtagagg
     4021 agtatagtat tgagggggta aatactacaa attttacttt ccattttagt tgtagctgtg
     4081 aaaatagtgg ttacgatatg tcggaaagaa tggcagatat tctacttggt gaaggaaaaa
     4141 aattatctcc taaaaattat cgtttaaaaa agagtgagca tctattcgat tttactagat
     4201 atctagatag aaattttgga ttttttcatc attcatatga tgaaaaaatc tccgaatatg
     4261 cgcctctcca tgcagtagag attaatgttg atgctggtta cgagtcaggc tggggaagga
     4321 cttttatgac agaggctgca acaaaatctg cttttttaga gtcattagag agatactgca
     4381 actctagaaa tagacgctat aaaaaattaa ttagagggtg tttttctgat ttatctaaaa
     4441 agtacaatac attaaatcct gttgattttt gtctttctaa taaagaatat agagatagtt
     4501 cctataaatt tcatgatttt gattctgaga aaaattatga ttggatattt ggtaaaaata
     4561 taactaccgg aaaaatttgc ttattgccct cgcaactagt attttataaa gactttagta
     4621 aaagcgaaga aaatgaaagg tttgtttatg aaacatcaaa tggatgtgct ttggggagta
     4681 gtattgagga agctattttt tatgggattt tagagattat tgaaagagat aattttttac
     4741 ttacatggta tttaaaagaa gaagtaaaac aattatcatg ggaatcttta ccatctcgcc
     4801 tacgtattat taatgaaaga ctaaaagaag ataactgtga gctcaaactt tttaatataa
     4861 ctaaagaagt gggcatacca gtaatttggg cattgtatat aaatcataat gaaaatgcta
     4921 gtattcgaag tttttcagct gcaggctgtc atattaatcc tttaagtgca attgaatcag
     4981 caatttttga agtaataact tctgtgccaa cttttgaaag attaattgaa gttagaaata
     5041 aagagtcatt tattgaggaa attcagaata atttttcttt aatacgagat attgacgatc
     5101 atatattgta tcatgctcat gcggattata aaaataaaat gcccttttta tttaaaaata
     5161 attatagtga atatcaaaat gaatttgatg agaatagaat ttttaatact tattcacatg
     5221 caacgcttac atcagattta caggcactaa taaaaattat tgaaaaaaaa tttggagata
     5281 tatatgtagt gaatataagt gataaagttt ccgaaaaatt tggactttat tgtgttaaag
     5341 tgttgattcc tggacttctt aatatgtcat ttggacattc aaatcggcgt gtaaatatag
     5401 agaggataaa aaattatgta gattatagag ggggaaatgt aaatagtatt acattgaatt
     5461 ccataaacca tcttccccat ccatttccat aagattatat ataaaggaat aataaatgaa
     5521 aagagattgg aaatcaaagc atttatttta tcatgatgtt caacaatttg ataaattggt
     5581 aatctattta tcaaaattta tggataaatt agttaatcag aatcagattg aaaaatattt
     5641 ctatattcgt tattgggaag gggggcctca tattcgatta agatatttgg tgagggatac
     5701 acaaaatttt gaaacaattg atagtatgat aaataagtat gcttataaat tttgggaaaa
     5761 taatcctcca atgaagttat taaataagac cgattataag aaaatttata atgagagtat
     5821 tgataacaac ggaaagatgg atagaaaatt gaggaaaaat gcgactattg aaaacattgc
     5881 ctatataccg gaaatagaga gatatggcgg ggaagaagct ctgatagtaa gcgaagatct
     5941 attttgtatc tgtagtcagg tttcaactgc gttacttaat gtggtagata actat
//
LOCUS       DAVBCG010000112         5940 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBCG010000112 DAVBCG010000000
VERSION     DAVBCG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.112"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(45..794)
                     /locus_tag="VQ495_001697"
     CDS             complement(45..794)
                     /locus_tag="VQ495_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2531627.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            927..3263
                     /locus_tag="VQ495_001698"
     CDS             927..3263
                     /locus_tag="VQ495_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2531628.1"
                     /translation="MDKNKIMGLTQREIKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3365..4042
                     /locus_tag="VQ495_001699"
     CDS             3365..4042
                     /locus_tag="VQ495_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2531629.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4029..4826
                     /locus_tag="VQ495_001700"
     CDS             4029..4826
                     /locus_tag="VQ495_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2531630.1"
                     /translation="MLASEVIHAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4843..>5940
                     /locus_tag="VQ495_001701"
     CDS             4843..>5940
                     /locus_tag="VQ495_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2531631.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKS"
BASE COUNT         1600 a         1194 c         1457 g         1689 t
ORIGIN      
        1 tatataaatg aaaaccttag ctgtttggcc aaggttcatt tcttctatgc aagttcttct
       61 cttttcttat ctgggttcca gatgaagctt gcgataaagc taaagatgat ggttaggata
      121 gcgagaataa tagcaaggat gagggcaggg atgcggataa accaccagag tggatttggt
      181 tttttatcat tgtgccattg tttcgtttct tcgtccagac gttggaattc tgtttgaata
      241 cgattggcga ctgtttcaat gccttcatca ttcattttct tgatatctga gatatcgatt
      301 ggatttccaa agttcatatc gacacgttca cggctaatca agcccttcaa agtcatggga
      361 ccggtgtagg taaccggcat gatacggacc ttggccattt tggcaatcag tgctgcgccc
      421 cccttgacat cgtttgagtg gcggctacca cttggaaaca tgatgagaga gcggtcactt
      481 tttttgagaa cgttgatagg atatttgatg gctgaggcgc tgggattttc acggtcgatg
      541 ggaaaggcgc cacacatacg aatccaccaa ccaaagatac ggttggtaaa gagttctttt
      601 tttgccataa agatgaactg ttttggcttg gtcgcaaagg ccatataaac aggatcccac
      661 caggtacggt gaggcgcaac taaaatataa ttttcatctt gattaggaat tttatcagta
      721 ttatgatagt gagcattgcc attgatggac cataagagca atacaactaa tccacgcaaa
      781 taagtataaa acatgcgatc tccttcgatt gttttcttgt tattattata ccttatcaaa
      841 ggagggctgg caaacttttc ccttgactag atgcatattt gggatgaaat tagaattctg
      901 ttaaaaaaaa tgatataata gaatttatgg ataaaaataa gattatggga ttaacccaaa
      961 gagaaatcaa ggaaagacag gctgagggtt tggtcaatga ctttaccgca tcagccagta
     1021 ccagcacttg gcaaatcgtt aaacgaaatg tctttaccct ttttaacgct ttgaactttg
     1081 ccattgcttt ggcccttgcc tttgtgcagg cttggagcaa tctggtcttc tttgctgtta
     1141 tctgctttaa cgctttttct gggattgtga ccgagctacg agccaaacac atggtggaca
     1201 agctcaatct catgaccaag gaaaaggtca aaaccatccg tgatggtcag gaagttgctc
     1261 ttaatcctga agaattagtg ctaggagatg tcattcgttt gtctgcagga gagcagattc
     1321 ctagtgatgc cttggttttg gaaggctttg cggaagtcaa tgaagccatg ttaacgggag
     1381 aaagtgattt ggtgcaaaag gaagttgacg gcttactttt gtcaggaagt ttcctagcca
     1441 gtgggtcagt tttatctcaa gttcaccatg tcggtgcaga caactatgct gccaaactca
     1501 tgcttgaggc taagaccgtt aaacccatca actcccgtat catgaaatcg ctggacaagt
     1561 tggctggttt tactgggaag attatcattc cctttggtct ggctctcttg ctggaagcct
     1621 tgcttttaaa aggcctgcct ctcaagtcat ccgttgtaaa ctcgtcgaca gctcttttgg
     1681 gaatgttgcc taagggaatt gcccttttga ccattacttc gctcttgact gcagtgatta
     1741 agttgggctt gaaaaaggtc ttggtgcagg agatgtactc tgttgagacc ttggcgcgcg
     1801 tggatatgct ctgtctggac aagacgggta ccatcaccca aggaaagatg caggtggagg
     1861 ctgttcttcc actgacggaa acttatggtg aagaggctgt tgccagcatc ctgaccagct
     1921 atatggccca tagtgaggat aaaaatccaa cagctcaagc cattcgccag agattccaag
     1981 gtcaagtagt ctatcctatg ctctctaatc ttcccttctc cagcgaccgc aagtggggag
     2041 ccatggagtt agaaggtttg ggaatagttt tcttaggggc acctgagatg ttgctggatt
     2101 ctgaggtccc agaagccaga gaggccttgg agagaggatc acgtgtcttg gtcttagctc
     2161 tcagtcagga gaaattagac catcacaaac cacagaaacc atctgatatt caggctctag
     2221 ccttgctgga aatcttggat cctattcgag agggagcggc agagacgctg aactatctcc
     2281 gttctcagga agtggggctc aagattatct ctggtgacaa tccagttacg gtatccagca
     2341 ttgcccagaa ggctggtttt gcggactatc acagctatgt cgattgttca aaaatcacgg
     2401 atgaggaatt gattgctctg gcggaggaga cagcgatttt cggacgtgtt tcccctcatc
     2461 aaaagaaact cattatccaa acgctgaaaa aagcgggtca tacaacagct atgactgggg
     2521 atggagttaa tgatatcctg gctcttcgtg aggcggattg ttctatcgta atggcggagg
     2581 gagacccggc aacccgtcag attgccaacc tggttctctt gaactcagac tttaatgatg
     2641 ttcctgagat tctttttgaa ggtcgtcgtg tggtcaataa cattgcccac atcgctccga
     2701 ttttcttgat aaagaccatc tattccttcc tgttagcagt catctgtatt gccagtgctt
     2761 tactaggtcg gtcagagtgg attttgattt tccccttcat tccgatccag attaccatga
     2821 ttgaccagtt tgtggaaggt ttcccaccat tcgttctgac ttttgagcga aatatcaaac
     2881 ctgttgagcc aaatttcctc agaaaatcca tgcttcgtgc cctaccaagt gccctcatgg
     2941 tcgtgtttag cgttcttttt gtgaaaatct ttggaacgag tcaaggttgg tctgagttag
     3001 aaatctcaac tctcctctat tatctcttgg ggtcaattgg tttcttatcc gtatttagag
     3061 cctgcatgcc attcactcta tggcgagtcc tcttgattgt ctggtcagta ggaggcttcc
     3121 tagccacagc tctcttccca agaatccaaa aactgcttga aatttcaacc ttgacagaac
     3181 aaacgttgcc tgtttatggt gtcatgatgt tggtctttac agtgattttc atcctgacta
     3241 gtcgctatca agctagaaaa taaagaaaga ctgcaatctg tggattgcag tttttttagg
     3301 tacaagattg ccagccgaaa tgtggtataa taagaggtaa tagagttttg gaaagtgaga
     3361 gaagatgatt tcaaagagat tagaattggt agcttccttt gtgtcacagg gggctatttt
     3421 actagatgtg ggaagtgacc atgcttatct gcctatcgag ttggttgaga gaggccaaat
     3481 caaaagcgct attgcaggtg aggtggtgga aggtccctat cagtctgcgg ttaaaaatgt
     3541 tgaggctcac ggcctaaagg agaaaatcca agtccgttta gccaatggct tggcagcttt
     3601 tgaagagact gaccaagtgt ctgtcattac cattgctggc atgggtggtc gtttgattgc
     3661 taggatttta gaagaaggtt tggggaagtt agctaatgta gagcgtttga tcctccagcc
     3721 caataatcgt gaagacgact tgcgtatctg gctacaggat catggattcc agattgtagc
     3781 agaaagcatc ttagaagaag ctggaaagtt ttatgagatt ttggtggtgg aagcaggaca
     3841 aatgaagcta tcagccagtg atgttcgctt tggtcccttc ttgtccaaag aagtcagtcc
     3901 agtatttgtc caaaaatggc aaaaagaagc tgagaagcta gagttcgctc tcggacaaat
     3961 cccagaaaaa aatctggaag aacgtcaagt tctagtagat aagattcaag ctatcaagga
     4021 ggtgctccat gttagcaagt gaagtgattc atgcttatga agccttttgc cctcaggaat
     4081 tttctatgga gggagacagc cgtggtctgc aaattggcac tttagacaag ggtatccaaa
     4141 gggtcatggt tgccctcgat attcgtgaag agacagtggc agaggccatt gaaaagggtg
     4201 tggacttgat tatcgtcaag cacgcgccta tctttcgtcc tatcaaggac ttgcttgcca
     4261 gccgtccgca aaatcagatt tacatcgacc tgattaagca tgacatcgca gtttatgtca
     4321 gccataccaa tattgatatc gttgaaaatg ggctcaatga ctggttttgt cagatgctag
     4381 gaatcgagga gacgacttat ctgcaggaaa caggtccaga acgtggaatt ggacgtattg
     4441 ggaatattca gcctcagaca ttttgggaat tggcccaaca ggtcaagcaa gtctttgacc
     4501 tagatagcct tcgaatggtg cattatcaag agaatgattt gcagaagcct atttcaagag
     4561 tagcaatttg tggtggaagc gggcagtctt tctataagga tgctttggca aagggggcag
     4621 atgtctatat cactggtgat atctactacc atactgctca ggatatgctg tctgatggct
     4681 tgttagcatt ggatccaggt cactatatcg aagtgctttt tgtggaaaaa atcgcagcac
     4741 tccttactca atggaaagag aaaaaaggct gggaactaga gattttacct agtcaagcat
     4801 cgaccaatcc tttccgtcat atctagttag aaagtgaaga caatgaaaaa agttgccatt
     4861 attggagcag gaattgtggg agcaacagct gcctactacc tctcgagaga aagtgaccta
     4921 gaggtgaccg tttttgacca tgagcaaggt caagccacca aggccgcagc aggaattatc
     4981 agtccttggt tttccaaacg ccgtaataaa gcctggtaca agatggcgcg cttgggggct
     5041 gatttttatg tggatttatt agctgattta gagaaatcag gacaaaaaat cgacttttac
     5101 cagcgttcgg gagtctttct cttgaaaaag gatgaatcca agttggaaga actttatcaa
     5161 ctggccctcc agcgcagaga agaatctccc ttgatagggc aattagccat tctggaccaa
     5221 gcctcagcta atgaattatt ccctggtttg cagggatttg accgcctgct ctatgcttct
     5281 ggtggagcga gagtagatgg ccaactcttg gtaactcgtc tgctagaagc cagtcatgtc
     5341 aagctggtca aagaaaaagt gactctgaca ccgttagcat caggctacca gattggtgaa
     5401 gaggagtttg agcaggttat tttggcgacg ggagcttggt tgggggacat gttagaaccc
     5461 ttgggttatg aagtagatgt tcgtcctcaa aaaggacaac tacgagatta tcagctggtc
     5521 caagacatgg aagcttaccc tgttgtcatg ccagaagggg agtgggattt gattcctttt
     5581 gcaggtggga aattgtcctt aggcgctacc cacgaaaatg acatgggatt tgatttgacg
     5641 gtagatgaaa ccttgctcca acaaatggag gaggccacct tgactcacta tctgattttg
     5701 gctgaagcta cttcaaaatc tgagcgtgtt ggaatccgtg cctacaccag tgatttctct
     5761 cctttctttg ggcaggtgcc tgacttaact ggtgtctatg cagccagtgg actaggttca
     5821 tcaggcctca caactggtcc tattattggt taccatttag ctcaactgat ccaagacaag
     5881 gagttgacct tggaccctga aaactaccca attgaaaact atgtcaaacg agtaaaaagc
//
LOCUS       DAVBCG010000113         5939 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBCG010000113 DAVBCG010000000
VERSION     DAVBCG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.113"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            405..1310
                     /locus_tag="VQ495_001702"
                     /pseudo
     CDS             405..1310
                     /locus_tag="VQ495_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1355..1795
                     /locus_tag="VQ495_001703"
     CDS             1355..1795
                     /locus_tag="VQ495_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531632.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            1804..2232
                     /locus_tag="VQ495_001704"
     CDS             1804..2232
                     /locus_tag="VQ495_001704"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531633.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            2245..3048
                     /locus_tag="VQ495_001705"
     CDS             2245..3048
                     /locus_tag="VQ495_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2531634.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(3081..3791)
                     /gene="treR"
                     /locus_tag="VQ495_001706"
     CDS             complement(3081..3791)
                     /gene="treR"
                     /locus_tag="VQ495_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2531635.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            3976..>5939
                     /gene="treP"
                     /locus_tag="VQ495_001707"
     CDS             3976..>5939
                     /gene="treP"
                     /locus_tag="VQ495_001707"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2531636.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATR"
BASE COUNT         1657 a         1104 c         1382 g         1796 t
ORIGIN      
        1 ctgaaagaat tttcgaaaaa ttttaaaaaa ttttgaaata ttattgacag gtgatgaaaa
       61 aggtggtagg ataggaagga tcaaaaaaga aagcagaaaa agaaatgaat aagagacaag
      121 ccgtaactat gaatcaaaac ttttacttta gctactttag atagtgtttg tagacatgtc
      181 accatggatg caaacagttc aagcaatttg tttgtatcta tgtggctaag atttttgatt
      241 gtggtccata tagatgaata tctaaatgga ccagctaagt tgtaacttgt taggttattt
      301 caagtacccg tttagatagt atctaggcgt ttttttgttt tatcttttct gagaaggagt
      361 ttcattatga aagttgttcg aaaatcacta gccccctctt ggtagtgggg atcctcttga
      421 cttctctgat cagtttgcat cagttgaagg cagataagaa aagagatgtc tttcgtatcg
      481 gtatttcgca gtttattacc caccagtcct tagacgctac tagagaagga tttgtggatg
      541 agctggccaa acaaggctat gttgaggggg aaaatattga gattgatttg caaaatgcac
      601 agggagaaca aagaaatcta aaaacgattt cccagcaact agcagaatcc agcgatgtcg
      661 ttctagccat cgcaaggcct tctgctcaga gcttggccaa tacgacgcaa acgacaccag
      721 ttatcttttc agctgtaaca gacccagtca gtgccaagtt ggttgagtca agagaacatc
      781 ctgggggcaa tgtaactggg acaagcgatc agtcatcaga tgccatttca acccaaatca
      841 atttgataaa gaaagtgttg ctaaaggcta aaacgattgg aatcctctat actcagagcg
      901 agcccaattc ggttgtttaa aaggatgaag ctaagcgatt tctgaaagaa aaaggcttta
      961 ccgttgttga aaaaacaatc ttggacagta ataacgtcaa ggcggcagca gagagtttga
     1021 tggcagaggt ggatatggtt tttgtcccaa ctgataatat catttcgtca accatggaaa
     1081 cagttaagca ggtttctatt aaacacaagg ttccagtatt cggtggttca acagaaatga
     1141 ttgctgttgg tggcttgtat aactacggaa ccaattatga agaattggga agacagacag
     1201 ctcgcatgct gattcgtgtt ttaaaaggtg aggaaccaga aaatatagca gttgagttgc
     1261 ctgaaaaact ggaattgcat actaatcaag aaatggcaga tgcattgtga attgatatta
     1321 gtaagttaga aggcaaggaa taaggaggat taagatgaat tttgtgttat ctagtttatc
     1381 agaaggtttg ttgtggtcga ttatggcaat tggggtctac ttgactttcc gtattttgga
     1441 tattgcggat atgactgctg agggagcctt tcctatgggg gcagcaatcg ttgtatcaca
     1501 gatacaggca ggggcaaatc cttggcttgc gactttactt gctttgctgg caggtatggt
     1561 agcaggtctt atatcaggaa tgctccacac caagatgaaa atcccagctc tcttgacagg
     1621 gattgtgacc ttgacagggc tctattcaat caatattaaa atcatgggca gtgtacccaa
     1681 tctttccttg ggagattctg caaccgtctt taaacagttg gcgagcttgg gtctgacaaa
     1741 tgaaggagct gttttctcac ttagtttagt ctgtctctta cttgtttggt tttgaccctt
     1801 ttgatgaaaa cagagattgg tttggtcttg cgttcgactg gggacaatat cccgatgagt
     1861 gaggccaatg gagtcaatgt aaacaccatg aagattgttg gttacatgat ttcaaccggt
     1921 ttgattgccc tatgtggttc cttgtttgcc caaaatgatg gattttcaga tgtgacttct
     1981 gggacaggaa ccattgttgt tggtttgagc tcggaagtct tgatacacga cttgaccatt
     2041 ggaggccgct tattatccat cggaatcggt gctattgttt accgtttgat tattttaaat
     2101 atctatgaaa ttccaaatct agatcaaaac ttggtacgtc tctttaatgc aatcttgctc
     2161 gctttggttt tatttgcacc agcattgcaa aagagattaa agattcgtgg tctgaaattg
     2221 agaaatgaat aggaggagaa agttatggca agtttgttaa cacttgaaaa tattcacaag
     2281 acatttgaag cagggacggt caatgagaac catgtcctca aaggattaga tttagaggtt
     2341 gaagagggag attttatctc tgtgattggt ggaaatggag caggaaaatc cactttgatg
     2401 aatatcttgg ctggtaatct atcggtggac gaaggggacc ttctactggc agggaaatcc
     2461 attaaacatc tgagtgtcag aaagagagcc aaggatattg cccgtgtttt tcaagatcct
     2521 aagatgggaa cagcttctcg tttgacgatt gaggagaata tggctattgc cttgagacgt
     2581 gggcaaaaga gagggcttgg ttggggcgtg aaggagaagg atagaatcca gttccaagag
     2641 gccttgaagg agttgaatat tggtcttgaa aaccgcttaa aagtagatac tcaatatctc
     2701 tcaggaggac aaagacaggc tttgacccta gtcatggcag ctctggtgaa actcaaactt
     2761 ttgcttttgg atgaacatac tgcggcactt gacccgaaaa ccagtcaaat ggtgatggac
     2821 ttgacgcaaa agattgtgga gtaccatcag tggacgactt tgatgattac ccatgatatg
     2881 aatcatgcga ttgagtacgg caatcgtctc attatgctct atcaaggcaa gatagtggtg
     2941 gatgtcaagg gagaagagaa aaagcatctg acagttgaag accttatgca tctcttccag
     3001 aaaaatagtg gccaaagtct ggtcagtgat gaattagttt tgggataaag aaaaaggctg
     3061 agatctggtg tctcagtctt ttatttcttg cgttttgcaa aatctacaaa tctaaatttt
     3121 tctaacttat ggcgactttc ggtaaactga aattggcgcc cgtcttggag atagactttt
     3181 gatttaatcg aaacgacata agggtctttg ccaatgtcca tgagaatctt gtctcggtcg
     3241 cttgagtggt caatggtgat ttccttctga gcataatcaa taaggagttt gaggccattt
     3301 tctatataag aatagataga ctgctcagca atttggcgag tgagatttgg gatgagttcc
     3361 atatccagat agtccgtatc cagaacggat accagatcat ccaccacacg ctggcggacc
     3421 accttccaaa ccatccgaaa ctctgggaaa ccggttatca gtgaggattt tttatcaata
     3481 ataatcttgt ccagactgac cacgttggtt ttagagcgca gtccaagttc tttaactagt
     3541 tcttggtagc tggttaggtt ggatacaggg aaattgacgg tttcttcttt gacgacttga
     3601 gaaccttgcc ctcttatctt tttgatcaat ccttcctctt ggaggagaga cagggccttt
     3661 cggacggtat cacgactcac ttgatattgc tccataaggt cgtgctcgct aggaaggaaa
     3721 tctccgacag cgtaagtctc gttttgaatg gtttcttgga tttgcttaaa taattgttgg
     3781 tatttcttca ttttgcctgc ttaaataggt ttttagtcta gtttaatgaa cacctgtatc
     3841 tattttacca tacaagtgct agaaaattta caaaaaaaca ccctttttat accaaacaag
     3901 ttgcagacaa gtttggtatc gtttacaata tacttatcaa atcaaacttg ctttgacaag
     3961 tataaggaga atcaaatggg aaaatttgaa caagaagcca aagatctgct tcaggcaatc
     4021 ggaggcaaag aaaatgtgac tgccgtaact cactgtgcaa cacggatgcg ctttgtttta
     4081 ggaaatgata agaaggctaa tgttaaagct atcgagtcaa ttccagctgt taaaggaacc
     4141 tttacaaatg caggtcaatt tcaggtaatc attggaaatg acgtgcccat cttttataat
     4201 gattttacag ctgtttcagg tattgagggt gtttccaaag aagcagccaa gtctgcagct
     4261 aagagtaatc aaaacgtggt ccaacgtgtc atgaccactc tggcggagat ttttactccg
     4321 attattccag ccttgatagt cggaggattg atcctcggtt tccgtaatgt cttggaaggt
     4381 gtccattggt cgatgttgga tggcaagacc atcacagaat cctctcagtt ttgggcaggt
     4441 gttaatcatt tcctctggtt gcctggtgaa gctatcttcc agttcttacc agtagggatt
     4501 acttggtctg tttctcgtaa gatgggaacc agccaaattt tgggaattgt tctcggaatc
     4561 tgtttggtat cgcctcagtt gctcaatgcc tatgcggttg cttcaacgcc agcagctgat
     4621 atcgcggcaa actgggtttg gaattttggc tattttactg ttaatcgtat cggttaccaa
     4681 gcccaagtta tcccagcctt gcttgcaggt ttgagtctat cttatcttga aatcttctgg
     4741 cgcaagcata tcccagaagt catttctatg atttttgtac ctttcttgtc attgattcca
     4801 gccttgattt tggctcatac tgttttggga ccaatcggtt ggacaattgg acaaggactt
     4861 tcatcagttg tcttggcagg tttaactggt ccagttaaat ggctcttcgg tgcaattttt
     4921 ggtgccctct acgctccatt tgtcatcaca ggtctgcacc atatgaccaa tgccattgat
     4981 acacaattga ttgcggatgc tggtggcact gccctatggc caatgattgc tctttctaat
     5041 attgctcaag gctcagccgt gtttgcctat tatttcatgc atcgccatga tgagcgtgag
     5101 gctcaggttt cacttcctgc aaccatttca gcctatctcg gtgttacaga accagctctt
     5161 tttggggtta acgtaaaata tatttatcca tttgttgctg ggatgactgg ttcagccctt
     5221 gcaggcatgt tatccgttac ttttaatgta actgcggctt ctattggtat cggtggtttg
     5281 ccaggtattc tctctattca acctcaatac atgctgccat ttgcaggaac tatgctagtt
     5341 gcgattgttg ttccaatgct cttgactttc ttcttccgca aggctggtct ctttacaaaa
     5401 acagagggcg atacgaactt gcaggcagaa ttcgttgctc aagaagaagc agaatttgtg
     5461 aaccatgaac cagtagaact tacttcggta gaaattatca gcccactaac tggccaagtg
     5521 aaagaattga gtcaagcgac ggatcctatt tttgcatcag gtgtcatggg gcaaggtcta
     5581 gtcattgaac caagccaagg tgagttgacc tctccagtta atgggacagt gacggttctt
     5641 ttccctacca agcatgccat cggcattgtc tctgacgagg gagttgaatt gctcatccac
     5701 atcggtatgg atacagtagg tcttgatggc aaaggttttg aaagtcttgt agtccaagga
     5761 gatcacgtta cagttggtca gcaactgatt cgttttgata tggatgtcat taaggctgca
     5821 ggtctggtga cagaaactcc tgttatcatc accaaccaag atgcttatac agcgactatt
     5881 cccggaactt atccgacaac gattcaagct ggagcatctc tcatggtcgc tacacgaat
//
LOCUS       DAVBCG010000114         5887 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBCG010000114 DAVBCG010000000
VERSION     DAVBCG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.114"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(119..751)
                     /locus_tag="VQ495_001708"
     CDS             complement(119..751)
                     /locus_tag="VQ495_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2531637.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ495_001709"
     CDS             complement(773..1645)
                     /gene="sdaAA"
                     /locus_tag="VQ495_001709"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2531638.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ495_001710"
     CDS             complement(1654..2325)
                     /gene="sdaAB"
                     /locus_tag="VQ495_001710"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2531639.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2567..3139
                     /locus_tag="VQ495_001711"
     CDS             2567..3139
                     /locus_tag="VQ495_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2531640.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(3191..4054)
                     /locus_tag="VQ495_001712"
     CDS             complement(3191..4054)
                     /locus_tag="VQ495_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531641.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            4520..4858
                     /locus_tag="VQ495_001713"
     CDS             4520..4858
                     /locus_tag="VQ495_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2531642.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            4836..5195
                     /locus_tag="VQ495_001714"
     CDS             4836..5195
                     /locus_tag="VQ495_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531643.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            5214..5426
                     /locus_tag="VQ495_001715"
     CDS             5214..5426
                     /locus_tag="VQ495_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531644.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
BASE COUNT         1819 a         1012 c         1229 g         1827 t
ORIGIN      
        1 atctacaacc ttaaaacagt gttttgagca gcccgcagct agtttcctag tttgctcttt
       61 gattttcatt gagtattact tcattttctt ctgaaataga gttgtcgccc agtctatgct
      121 attgaaaata cgccaaaact tctaagggtt tgtgagcgat ataatcaggt tgatagttta
      181 gtagatctgc ttgctctcca aatccccaag tgacggccaa tttctgaata cctgtttctc
      241 gagctcccag catatcaaac ttggtatctc cgatgatgat ggcttgttct ggtgctagtt
      301 gatgtgtctg taaggcttgg tgaatgacat ctgccttatg gggtgcttca gggctagaac
      361 cataaatacc atcaaagaaa tgatggattt ccaagttttt ggccatgtct tgagcagtag
      421 atgtattctt tgtcgtggtg atgtagagtg gataactgct cgataactcc tcaagcaagt
      481 ctataatctg aggaaagagt tgagcttcat agatgccttt tgccttatag taagaacgat
      541 atatctgcat ggcttcagaa atttggtctt tggacaggca ggtcgcaaaa ctactttcga
      601 gaggtggtcc cataaaacca cgaatagttt tggcatcagg gctaggcacc cccagctctt
      661 taaaggtata ggtaaaggca ttgtgaatcc cgatagaact atcaacgagg gttccatcca
      721 aatcgaaaaa aatcgctgtg atagaggtca tggtttctcc tatttgataa gcttattctc
      781 cgaaaatctc tttttggagg cgacgaccag taggggtggt agcgagtcca ccttcagctg
      841 tttcacgaaa ggcagttggc atgcttgctc ctacttggta catggcatcg atcacttcat
      901 ccacagggat tttagattcg atacctgcca aggccatgtc tgctgcgatg aaagcaaagc
      961 tagctcccat ggcattacgt ttgacacagg gaacttcgac caaacctgca acagggtcac
     1021 agatgaggcc tagcatattt ttaatgacaa aggcaatagc ttgactggcc tgataaggtg
     1081 ttccacctgc agccagagtc aaggcggcag cactcatagc agaggctgaa ccaacttcag
     1141 cttgacaccc accctcagca cctgagatgg aggcattgtt tgcgatgact agtccaaagg
     1201 caccagcagc aaagaggaaa tccaattgtt gctcgtggct gaggtctaat ttttcaatag
     1261 cagcagtgag aacggaaggg agacaaccgg cacttcctgc ggtaggagta gcgcagacca
     1321 agcccatttt agcattgtgt tcattgactg cgatagcatt tcgggcagca gagagaatcg
     1381 tgtaatctga taaagttttt tcgtttttga tgtagtgatc caatttggca gcatctccac
     1441 ctgtcaggcc actacgagat ttattttcat tgaggccaag ttggacagag gctttcataa
     1501 cttccagatt gcgttccatg agaaggaaga cttcttcacg ttcgcgaccg gtcaattcaa
     1561 actctgttgt aatcatgagt tctgcgacat ttccttgaaa gtccagatct gcttgctcga
     1621 ccaattcttt gatagaataa aacatgcttc ctcctattta aagaaattga cattgtggag
     1681 atgagggatt tttcgaattt cttcgatagc ctcatcacag ttgcgactgt caacttcgat
     1741 aatcataatg gctttttcac cagctttttc acgagtgaca ttcatctggg cgatattgat
     1801 accatagcgg gaaagcgcct ctgtaacaag ggcaatcata cctggaatat cttgatgaac
     1861 gatgatgata gtcggtgtat tcatattgag agagacggca aaaccattga gttcggttac
     1921 ctgaatattt cctccaccga tagaaatacc agtcacgctg atggtcttgt gggcattttt
     1981 aacagtaatt ttagtggtgt tagggtgagg ggcattgctg tctttctgaa tggtccagac
     2041 aatcttgata ccacgcttgt gggcaatttc cagactattt ggaatttcag gatcatctgt
     2101 atccattcct aaaatacctg caacaagggc taggtctgtt ccgtgaccac gataggtctt
     2161 ggcaaatgag ttaaaaagtt ggaattcaac ttctgtcgga gtatcatcaa aaatggaaga
     2221 gacaatcttc ccaatacgaa cagcaccagc ggtatggcta ctagatgggc caatcataac
     2281 tggtccgatg atatcaaaga cagattgaaa acgaagtgat ttcatcagtt tccccttata
     2341 aaaattctta tctctattat atcaaagaat gaggggcttg gctttaattg tggatgaaaa
     2401 cctttctaat acctcaaata gcataaaaat agtatctttt atgacaaaaa acaccttatt
     2461 tagggaaata aaaaataatt ttgtaatatt tctacataaa agtgtcaaga aacagtaata
     2521 tttaaagggt atgatagaac tatagaaaga aggagaattt tcgaatatga aatcaataac
     2581 taaaaagatt aaagcaactc ttgcaggagt agctgccttg tttgcagtat tttctccatc
     2641 atttgtatct gctcaagaat catcaactta cactgttaaa gaaggtgata cactttcaga
     2701 aatcgctgaa actcacaaca caacagttga aaaattggca gaaaacaacc acattgataa
     2761 cattcatttg atttatgttg atcaagagtt ggttatcgat ggccctgtag cgcctgttgc
     2821 aacaccagcg ccagctactt atgcggcacc agccgctcaa gatgaaactg tttcagctcc
     2881 agtagtaagt gaaacagttg tttcaactgt aagcggatct gaagcagaag ccaaagaatg
     2941 gatcgctcaa aaagaatcag gtggtagcta tacagctaca aatggacgtt atatcggacg
     3001 ttaccaatta acagattcat acctgaacgg tgactactca gctgaaaacc aagaacgtgt
     3061 agcagatgcc tacgttgcag gacgttacgg ttcatggact gctgctaaaa acttctggct
     3121 taacaatggc tggtattaag aactaacgaa caaataatat gtaaagagtc gaggaaatcc
     3181 ctcggctttt ttatctttcc ttcgcttggt aatagatctc tacttctttt ttgagatgag
     3241 acagcataga agtttcctcg gttagctcca gaagggagaa ccctttttgg ataagtttag
     3301 aatcgtctgt acaaatccca atacggacat agcagatagc cagcctatcg tagcttttct
     3361 tattcttggc gtcctccagt aaagtgtagc agatttgctt acacatgttt ttatcttgat
     3421 tgtatagata gagtgtggaa aggtttaata gaattgtcat tcgcaagtca tataaatgtt
     3481 gatagttttt atagacttct aggcgttgca atatttttcc agtgatgaga tggaggtatt
     3541 caatgggaaa actgaaaaga atggtattga ggatttttag atcactctca taccatgtat
     3601 cttgtttttc aatttttttc caaagttttt tgacaacctg ttctgcgtgt tttgatagtt
     3661 cccctgcccc gtgttgacgg agataaataa caacttcaag catatctctg atttcttcta
     3721 tagggagatc gtggtgggtc ttgaggtagt cttggcattt ttgaaataaa ttgacaagat
     3781 cgctggtccc gataatggaa cgcatattga gataggtttg cataatctct gtgcgttgac
     3841 ttggttgata aagttgacag atatattcaa actcttcaaa actcatattg atttgacgga
     3901 gaagaaattc catattttca tattttgggg ttgtcttgcc actttcgatt tttgataagc
     3961 tggttcttga gagaacaccc ccacagacct cttcttgggt cagccccttt gactcacgta
     4021 tttctttata gacctttccg aaatcataac gcatgatttt ctcctttctg tgaaaaaagt
     4081 taacaaacaa taaaattttg ttaaaaatat tgtatcatag caatgtaaaa agtaaaaaag
     4141 aaagtcgaaa taaatttgtg aaaaaagtta acgtaatttt aaaatgagct taataagagg
     4201 tatgatgtag gtagaaaata aagataggag aaaaagtatg aataaaaaaa tattttgtat
     4261 tctagtatgt attatatcat tgattagtct ggccataatc tttccatggg gctggtcgat
     4321 ataatgtaag ggagcttgag ttgttaaagg aggcttagtc cttggacaaa aatctgctgt
     4381 actacaatgc ttggaaagat atcaagaaca aagaatgata cgattgaaaa taaactgatt
     4441 caagaactac tgggcagtga ttgaggtttg ggtgacatca aagaaaagtt agattaattc
     4501 taaagaattg agtatttcta tgttaaaaaa atatccttgt acgatgcaac atgatcagtc
     4561 agattgtgct gcggcagttg tttcaacagt tcttttatct tacaaaaagg aactatcaat
     4621 catgaagatt cgggaaatta ttggtacaga catgtatgga acgactgtca gtggtattgt
     4681 ttcaggtctg aataagttga attttacagt aaaagctgtt cgagtagcac tggaagattt
     4741 gactccaaaa ttaacatttc ctgcgattct tcaagttaag aatgatttag gtcaaaatca
     4801 ttttgtggta ttacatagta taaaggagaa aataaatgga actcgtatta ccaaataatt
     4861 atgttgtgat tgatgaagaa gagatgatgt accttgatgg gggggctata tatataccca
     4921 ggtgggcaat tacaggagcc attactggtg cagcatatgc agcattagca gcagcaggag
     4981 gtggaggcct tcaactagtt cttgcatctt atggattacg ctccgcactg gtagctggga
     5041 ttgttaaagg tttaggagta ttaggaattc atattggaaa tgcttttgca aatactgtta
     5101 ttagaagtat tgcatctgct ggaattggtg ctggagctga ttggatttta ccaatattat
     5161 tgatggctgg gatgggcgac gtgataatca attgagaata ggataatagt attatgatgg
     5221 ttgttttaat ttcagttatt gttgcattcc tgtaccataa aattacagag tatgtcaaca
     5281 acaggaaaat tgatattgtt atttttctat cattgctgat ttctcatttt atttggaatt
     5341 actatttttt accaattgaa atattgatag caatcagtgg agcaaattta ttattaagtt
     5401 ttttaaaaaa attacagatt aaataatatt attccatatg acatatcaac taggtcatta
     5461 gattttattg aagatataaa ttaaaaacga ggttgggaaa aaactcaatt tgaaagtgaa
     5521 attgagtaaa tttttctatt atataacgca tattatcaag atttttgaac acctgataat
     5581 atgcactttt cttattttaa agattttttg cctttcttca tttcttgaat gaattggaga
     5641 gatgacttta tttaggtgtt tgacattact atagatagga tttagcagta ctaatatgaa
     5701 ctcatattat aggtgcagac ttaaattttg gaagcaagct aaaggatatc ttatctaggt
     5761 aactaagtgc tataaacaaa gtcaacgatc acttgttagg aaacgatcct aactatgctt
     5821 aataaaaaag gagaaaataa atggaactcg tattaccaaa taattatgtt gttcttgagc
     5881 aagaaga
//
LOCUS       DAVBCG010000115         5773 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBCG010000115 DAVBCG010000000
VERSION     DAVBCG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.115"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            203..751
                     /locus_tag="VQ495_001716"
     CDS             203..751
                     /locus_tag="VQ495_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2531645.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(988..>5773)
                     /locus_tag="VQ495_001717"
     CDS             complement(988..>5773)
                     /locus_tag="VQ495_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2531646.1"
                     /translation="QGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKG
                     AQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRND
                     ADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKG
                     EDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTV
                     IFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHT
                     ITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDE
                     LVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTW
                     EPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQ
                     GERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDG
                     KSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNG
                     KNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTT
                     TIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIK
                     GPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQG
                     KDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHST
                     DITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGK
                     YTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPL
                     AVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQD
                     GQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRI
                     RRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITT
                     KDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKE
                     TVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPD
                     GSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETT
                     VRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVT
                     GADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPT
                     VTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGT
                     VTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVK
                     PVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVA
                     PTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
BASE COUNT         1242 a         1337 c         1044 g         2150 t
ORIGIN      
        1 attctatttc tcccttcaaa caaaaccgat tttgaaagtg aaacagttct tactttttca
       61 gtcacaaatg actagagttt ccgaaatttt acaattttca aataaactct gagcaaattt
      121 cttgcaagtt cttttgtttt gttgtaatgt attttataac aacgagagag ttctcaaaat
      181 ttgaagaaaa aggagacaca tcatgtctaa aaaagtatta tttatcgtcg gatcactacg
      241 tcaaggttct ttcaaccacc aaatggcgct cgaagctgag aaagcacttg ctggtaaagc
      301 ggaagttagc taccttgatt attcagccct tcctctcttc agccaagatt tggaagttcc
      361 aacacatcca gctgtagctg ctgctcgtga agcagttctc gttgcggatg ctatctggat
      421 tttctctcca gtctacaact tctctatccc tggtacagta aaaaacttgc ttgactggct
      481 atctcgtgcc cttgacttgt ctgatacgcg tggcgtttct gcccttcaag acaagtttgt
      541 caccgtatca tctgtagcca atgcagggca cgatcaactt ttcgctatct acaaagacct
      601 cttgccattt atccgtacac aaggcgttgg tgatttcact gctgcacgtg ttaatgactc
      661 tgcctgggca gacggaaaat tggttcttga agaaacagtc ctaaactcac ttgaaaaaca
      721 agctcaagac ttggtcgaag cgattcagta aaaaatagaa aattttagaa agtctaaatc
      781 aattatagcc acctaatagg gtggcttttt atttctcaat caagattttc agacaaacat
      841 cagccatcca aaaaaacctt gagcgttacc actcaaggtt tttagcagtc ttttccatca
      901 taggaaaatc attctaagaa tattctttca ttcgagatct atcacttatc atcttctttc
      961 gagaaattag tgactgcttt tggctgatta agcttctttt cttcgtccta catataggct
     1021 caatagaata ccgctaccta aaagaacaga gatataatca gattggcttc cagtttcagg
     1081 taatttgtcc ccacggtttt ctttacctgt agttggtgct actgggttag catttactgg
     1141 agttgctggt tgagccggtg ttggtactac tggttgttct ggaaccgctg gtactggttt
     1201 aaccggtact tctggtgttg gaacagctgg agttggttgt actggcactt ctggtgtcgg
     1261 tacatttggt gttggttgcg ctggcacagg tttaaccggt acttctggtg tcggcacatt
     1321 tggtgttgga ggaactggtt tgtcatttga tggtgttact ggtttgtctt ggcatccaca
     1381 ttttccatct ttaccgtctt taccgtcttt gccgtctcga ccatcacgtc catctctacc
     1441 atctttaata actgtttttg tcacagttcc atcagaattg atgattgtga ttgtatgact
     1501 accatcacca ttatcttcaa cagaaacttt tggtgattta ccatctttac cgtctttaat
     1561 caccatttct gtcttacttc catctggatt agtgattgta acaacgtgag ttccatcgtt
     1621 attattttta acagttacag ttggtgaaac gctatctttg ccgtctttac catctttgat
     1681 aatagatttg tattcacgac catcagaatc tacgattgtt aatgtatgag ttccatctcc
     1741 gttatcaacg atgttcgctt ttggtgattt accatcaaag attgtagttg tcgttccttt
     1801 accatcagca cctgtcactt taatagtgtg tgaaccatcg ttgttatcaa taacttcaag
     1861 ttttggttct ctaccatcac gaactgtagt tgttgtagtt gttccgtcac catttagaac
     1921 agagatctta tgagttccat tttgttcatc agttatagtc acttttggtg atttaccatc
     1981 acgaactgtg gtttcagttg tcacaccttc tggatttgta ataacgattg tatgacttcc
     2041 gtctccatta tcacgaacag atgcggttgg agtacgtccg tcaacaccgt cacgtccatt
     2101 cttaacaaca aactcattct tagttccgtc tgggttagtg attgttactg tgtggcttcc
     2161 atctggattt tcagtagttg ttgctgttgc agctttaccg tctttaccat ctttgatgat
     2221 agcgttggta acattgccgt ctccatctgt aactttaaca gtatgagttc catcgttgtt
     2281 atccgttact tcaactttag gagtcttacc gtctttaccg tttttaacaa cagtttcttt
     2341 agttgaacca tctggatttg tcaccgtaat tgtgtggctt ccatctgggt tttctgttgt
     2401 agtgatattc gcagttttac cgtctttacc atctttaaca actgttgttg attcagaacc
     2461 atctgggttt tgaactgtga tagtgtgagt tccatctcca ttatcttttg tagtaatagt
     2521 tggagctgaa ccatcacgtc catctatacc gttataaata tctttactat ttaatacttt
     2581 atcgacacct tcgtcgtata ccccattgtc attaacatca tcataaactg taacacggat
     2641 accgttacgc gttggagttg cagcagcatc tgtatcgcgt ctaattcttg aaggtgaagt
     2701 aatatcttct actttgattt tcggtgcacg gccatcttta ccattcttac catcttttac
     2761 agtgaaggtt actggatctt tacctgatgt tgtgatagtt acaacagtgc tttggccatc
     2821 ttgtccacgt tgagtagtga ttgtaggagt ttgtccatcc tgaccggttg caccagtttc
     2881 tcccttagca ccatcttgaa caaagactgt agttggttgt gaaccatcat ggttatcaac
     2941 agtgatttct acacctttat gccctgcttc atctttacga acagctgtaa ctgtaggtga
     3001 ttttccatct actccatctc taattaccgc aagtggtttt tctggattta atggttgtcc
     3061 tgggttagct ggatcttctt ggtaaacttt agtttcttta ccatccttga cagtaagcaa
     3121 tgatttacca tctcgtccgt ccgcgccttt agcaccatct ttaatcattt ctgaacctag
     3181 aagttcatca gttcctggtg tatatttgcc gtcattgttt tcatctgtat agaatgtcac
     3241 aattgtatga gatggatttc cgttttttcc ttggacacga agtaaatcaa cttttggagt
     3301 tttaccgttc agaccatttt ctccgtcctt aacatttact gtaactggat ctttacctgg
     3361 aacagtgaat gtaatgtcag tactatggcc atctttacct ggagttacag ttacttctgg
     3421 agtcttacca tcttctccgc gttcaccttt gtcaccctta gggcctttga tgttgccctc
     3481 tttatcccag ttgccgttat tcttaacgaa gacgtcgcct gtttctgtat taacgtattt
     3541 atcgccgtct ttaccttgat gtgcttctgg gttagctttg ccgttcaaca cgtctttacc
     3601 gtcacgtcca tcacgaccta ctgcgccatc cgcaccttta gctccttgcg ctccagttag
     3661 gccgcgttcg ccttggtctc ctttttcgcc tttagcacca tctgcacctt tgtccccttt
     3721 agggcctttg atgttgcctt ctttatccca gtttccattg tttttaacat agacatcacc
     3781 ggtttcggta ttaatatatt tatctccatt tttaccttgg tttggttgtg gatttacttt
     3841 tccgtttaat acatctttac catcacgacc tgcttgtcct gtagctccat cacggatcgt
     3901 agttgtcaca tctggacttc ctggattatg aaccgttaat gtatgtgtgc cgttttgccc
     3961 ctcagttatt gttactgttg gggttttccc atcagcaccc ttttgtccat cctctacaaa
     4021 tgttgtttgt ggacgacctc ctactggtgt aattgttact aatacaccat ttttgccatt
     4081 ttgcacactt cgtgtcgctg taattgttgg tgattgtcca ttagtaccgt ttcttccatc
     4141 agtgatagtt gctaatggtt ggtttggatt aagtggttgt cctggtctag ccggatcctc
     4201 tacatagact tttgtttcag ttccattttt aacagcaatt agagattttc catctttacc
     4261 tggagttcct ggattacctt gttctccttt tggtccttgt ggtcccgctg gtcctctttg
     4321 tccatctgcg ccttttggtc cttgagctcc atcacgacct tgaagacctt gttcaccttt
     4381 gtcacctttg gcaccttgcg ctccagttaa accgcgttca ccttggtctc ctttttcacc
     4441 tttagatcca gttagaccgc gttcaccctg atctcccttt tcacccttga caccttgcgc
     4501 tccagtcagg ccgcgttctc cttgggctcc tttttcaccc ttggcaccat ctgcaccttt
     4561 gtcacctttc ggtcctttga tgtttcctgc tggttcccaa gtgttacctt tcttaacgaa
     4621 tacatctcct gtttgtgtat ttacgaatgt gtctccatct ttaccttctg ttgttgggtc
     4681 tacttttccg tttaatactt cttttccatc acgtccagat gctcctgcag ctccgtcttg
     4741 tccgtcttta actactgttg tttgaactaa ctcgtctttt cctgctgtgt aacttccatc
     4801 tccatctttg tcaatgtaga atgttaatgt tgtttgtttc ttcgcatcat ctcgttctgc
     4861 tttgactttt ggtgtttttc catcttcacc gtttttaacg attgttgtta atgatggtct
     4921 gttgtctggt tgtgtgattg tgatggtgtg tgttccgtta ttgttatctg taactgttac
     4981 ttctggtgtg aatccatctt ttccgttatc acctttgtca ccttttggtc cttgttttcc
     5041 atctgcgata acaacttctt taagttttgt atctccttca tcaaattgat tattatcatt
     5101 tagatcataa tagaaaataa cagttgttgt tttatctgct tcatttctat gagtagaaac
     5161 aagagggctt gcaccatcaa atccatctct tactactgta gtagttatac cacctttacc
     5221 atcgttaatt gtaattgtat gcgttccatc ttgaccatca gttactttaa ctgttggggt
     5281 tttcccatct tctccttttg gtccttgttc accatcacgc ccttgaagac cttgttcacc
     5341 tttgtcccct ttttcacctt tagcaccatc tgcaattaca acttctttta atttcttatc
     5401 agaagcatct aattcattgt ttccattttt atcgtaatag aagataacag ttgttgtctt
     5461 atctgcgtca tttcgttgag tcgcaactaa tggacttgcg ccatcgaaac catctcttac
     5521 aacagtactt gtaacattac ctctaccgtc gttaatagtg atagtatgag tgccgtcatt
     5581 tttattatct ttaacggtta cggttggagt tacaccgtca cgacctgcct gaccttgggc
     5641 tcctttttca cctttagctc cagttaaacc acgttcgcct cggtctcctt tttcaccttg
     5701 ggctccagtg agaccgcgtt caccttggtc ccctttttca cctttagctc cagttaaacc
     5761 gcgttcacct tgg
//
LOCUS       DAVBCG010000116         5771 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBCG010000116 DAVBCG010000000
VERSION     DAVBCG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.116"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(94..783)
                     /gene="rplA"
                     /locus_tag="VQ495_001718"
     CDS             complement(94..783)
                     /gene="rplA"
                     /locus_tag="VQ495_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2531647.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMGLVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(990..1415)
                     /gene="rplK"
                     /locus_tag="VQ495_001719"
     CDS             complement(990..1415)
                     /gene="rplK"
                     /locus_tag="VQ495_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2531648.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            complement(1538..2254)
                     /locus_tag="VQ495_001720"
     CDS             complement(1538..2254)
                     /locus_tag="VQ495_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2531649.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            complement(2257..2727)
                     /locus_tag="VQ495_001721"
     CDS             complement(2257..2727)
                     /locus_tag="VQ495_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2531650.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            complement(2729..3766)
                     /locus_tag="VQ495_001722"
     CDS             complement(2729..3766)
                     /locus_tag="VQ495_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2531651.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(3864..5189)
                     /gene="brnQ"
                     /locus_tag="VQ495_001723"
     CDS             complement(3864..5189)
                     /gene="brnQ"
                     /locus_tag="VQ495_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2531652.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5360..5452)
                     /locus_tag="VQ495_001724"
     CDS             complement(5360..5452)
                     /locus_tag="VQ495_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2531653.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(5495..5647)
                     /locus_tag="VQ495_001725"
     CDS             complement(5495..5647)
                     /locus_tag="VQ495_001725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2531654.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
BASE COUNT         1656 a         1295 c         1092 g         1728 t
ORIGIN      
        1 gacttacacc tcagatatac gacatgcccg tcgtacatca aaaaaagttg agacatagcg
       61 tcttcaactt ttttacatta ctttaactac tgattaaagt gagtttacgt caactttgat
      121 accaacacct tgagtagttg tgatagtcaa gtttgttacg taagttcctt tagctgtagc
      181 tggttttgct ttttggattg tttcgttgaa agctttaaag ttttcaacca atttttcagc
      241 ttcaaatgat actttaccga tgattgcttg aacgttacct gcacggtcag cacggtaagt
      301 gattttacca cctttagact cttcaactgc tttagcaaca tccattgtta cagtaccagt
      361 tttagggttt ggcatcaagt tacgtggtcc aaggacacgt ccaagacgtc caacaagacc
      421 catcatatca ggtgtagcga taactacgtc gaagtccaac caaccgtcgt tgattttagc
      481 aacaaggtca tcttcaccaa caaagtctgc accagcagct tttgcttctt cagcttttgc
      541 accacgtgcg aaaacaagaa cgcgtgaagt tttaccagta ccgtttggca ataccattgc
      601 tccacggatt tgttgatcag cttttttaac gtcgatgttc aagttgtaag caacttctac
      661 agttgcgtca aattttgcaa agtttgtttc ttttgcaagt gctacagctt cttctacgct
      721 gtatgctttt gtgctgtcga ttttctcaag agcagcacga agttgtttgc tttttttagc
      781 cattttctat tctccttgta agtggttcaa tcgattttca tctcccacgt cacttctcga
      841 aatgatgaag tcttgcgggt tttcagtcta ttagattctt ttcctacggc cttaagctcc
      901 cttgctgtac ccaagtacaa gcatcagtca cttgcctagt atgaaaagca actaatagac
      961 tgctgtgaga ttttctactg tgttattgat tagtcaataa cagtgaatcc catagaacga
     1021 gcagtacctt cgatcatacg cactgcagac tctacgtttg ctgcgttcaa atctggcatc
     1081 ttagtttctg caatttcttg tacttgtgca cgagtaactg tagcaacttt agttttatta
     1141 ggtgtacctg atcctttttc aacacctgca gcttttttca aaagaacagc agctggtggt
     1201 gttttcgtaa cgaaagtaaa tgatttatct tcgtaaactg agataacaac tggaatgatc
     1261 ataccagctt ggtcagctgt acgagcgttg aactcttttg tgaatcccat gatgttgata
     1321 ccagcttgac caagagcagg tccaaccggt ggagctggtg tagctttacc agcagggatt
     1381 tgcaatttta caagtttttc gactttttta gccattttta aatcctcctt tgtggttttg
     1441 gcggtaatta gagattttta cctcccacaa gtatgttttt cacataccca tctattatac
     1501 actatttatc tggaattgca agagaaaagt ttatttttta atcgacgtaa tctccacctt
     1561 caaagccgaa atactcttcc aaactgaggc cactagctgc aatctcttta gcctcttttc
     1621 cgacataacg aaggtgccat tcctcagcca tatagcctgt ttccttttcc ttacctttga
     1681 gataacgtac aacaaagcca taatcagccg catgatccaa gagccattgg gccgcttttt
     1741 cttctgtcac caaatcacca tttgtcccaa tcacatcaaa ggctaaacct gtctggtgct
     1801 cgctatagcc aggacgggca gaataacggt cggcagcttc tttcccatct tgattgacat
     1861 aatcttgata gagcttagtc tgagtttcat aacttctaaa gccactgtaa tgatcgctaa
     1921 tggggaaacc tgcctcttgc atagctttga tgagcttgac caactcagcc ttggctgttg
     1981 gattttcccc tggattgtag tcttttgaca atggatagtg tttgttggct acgatgattt
     2041 catcgtattt cccttgaatg ctgtaatagt ctcctttgtt aaccacttct gcttttttct
     2101 gggcagctcc ttgagactta cttcctactg ttccatcagc tttagcagtt tgctctgtct
     2161 tagctgctcc atcttcattt tttgcttttt cttgtgaaca agctgctaga ctcaaggcta
     2221 gcaaggctgt caagacaagg tatctttttt tcatctttac tcctttattt ctaatagttt
     2281 atctacttct gatgataaac gttcgactaa tcttttaatc tcatcaggtt ttgattggca
     2341 gatttctgct gtcatttctt caaagggaat ccaccatact ggaccacgtc cattgaggcc
     2401 ataaccattc atatcacctc ttcgtctagg aaacagatgc caatggagat gagcatcgcc
     2461 atttcctagt agttcgatat tcattttctc agcagcaaag gccttggcaa ctgcctcttg
     2521 gaccacactc atttcttcta gaaaacgtat tctagttttc ttttctaaat gatgcaattc
     2581 gctgacatgt tccttggcta gaaagagact atagcctgca aaatactggt ggtctccaat
     2641 cacaagatag cctgtttcca actctttgac aaaataggga ttttctccct tcttgatgag
     2701 ctcaattctc tggcaaatca aacacatatt agtctaccaa tgcactctta agataggcat
     2761 cgaccattcg ttctgttgcg accaccgagt caatatgagt gcgctcataa gaatgactag
     2821 actcgatacc cgcaccaaga agggcatgct tgacctctgc ccctgcagac atagccgctg
     2881 aagcatccga accataaaat ggatagatgt ccagcttaaa aggaatatct tgctctttag
     2941 ccaaggcaac caagtgttga cggaagtcat agtgataagg acctgaggca tccttgacac
     3001 agatagatac tgtgtactca tttgtctgct ggtcatctcc catagctccc atatccacag
     3061 ccagatattc tactacctga gcaggaatgt tagaattagc accatgtcct acttcttcaa
     3121 aaactgaaaa agcaaaatga gttgttactg gcaattcaat cttctctttc ttataaatac
     3181 gaagcaggtt gagcaacatc gctgcgctga ccttatcatc caaatgacga gacttgataa
     3241 aacctgtatc agtcacaaca gttcgtggat cgaagctgat aaaatcaccg acctcaatcc
     3301 caagagcacg agtttctttt tcattgctta cttttacatc caaacgcact tccatattgt
     3361 cctgcgtgcg ttctgcagtt cctgcatcct tgtagacatg gcaagaagtt tggtggatga
     3421 ggatggttcc taataccttt tgacctgtgc tagccacatg aacggtacag ttttcacctt
     3481 caatcatgtt ccaaggaaaa ccaccgatac ggtccaattt aagacgacca tctggtttga
     3541 cagcacgaac aatagcaccc agtgtgtcca catgggcagt cacatagcga tgttgctcat
     3601 cattttgacc tttgatggtc acattgacac cacccttggc tgtgcgaact ggctggtaac
     3661 caaaaccttc tagagtcttg actaaataat tcgaaatttc acgagtgaag cccgttggcg
     3721 atgcgatggc tgtcagttct ttgatatatt ctactgtttg attcatttct tcacctcttt
     3781 cgctacctat tataacacat ttatcatcgg ataaaaaaag aaaatcactc ctgtagactt
     3841 ggctacaaaa gtgatttaag tgattattcc atttcaaaaa catcgctttc ttgcttgttt
     3901 ggtagaacca atgagagcaa gattccaaca atggctggca ccaaccatgg gagagatgcc
     3961 ttggcaaaag gaagaacgct aacaagattt gcaagaaact caaccttaaa cgagcttcct
     4021 agtacgcttg caatggcaat aactgtaacc acagcaattg tcaactgcat acctggtttt
     4081 gaaagggcaa caaatttgtt gacaatgaca atcataacga tagcaatcgt gattgggtac
     4141 aagataacca gtactggaat tgagtacttg ataatcgcat caagacccaa attggcaata
     4201 gcaaatccaa tcaaggtaaa ggctgtcgca taaaccttgt agctgatttg tgggaagcgc
     4261 tcattaaaga actcagctgt tgacacaatc aaaccaacag tcgttgtgaa gcaggttacg
     4321 gtaaccatag ctgcaaggaa gagttgagct gttgagccaa agatttcttg agtggcttgt
     4381 gacaagatgt aaacacctgg tgttccaccc ttcatcgctt cagctggtac tgggaaatga
     4441 tttccaagaa aacctaaacc gatgtaaaga gcgctgaagg caagggcaac aacgatacca
     4501 acaacccaaa tagttgaaat gtattctttc ttacttgaaa atccaagttg tttcaaggtt
     4561 tgaactgcga ttacgctaaa ggccactgag gcaagggcgt ccaaggtatt ataaccttct
     4621 aggaaacctg taccaaaggc agaagcttga taagcagctg aagcagcttg aggacttgtt
     4681 ccaccatatt tgatagctcc cagaacgacc aagataacaa tcaaaattgc aaagactggc
     4741 gttaaaatac gtccaatgcg gtctaagatt tttgatggat taagcgaaat caaataggct
     4801 gccgcaaaat acagaaccgt aaatacaatc aagccaagtc ctttatttgc atccgacaaa
     4861 agggggctaa tccctacttc gtaagctgtt gtagcagtac gtggggtagc aaagaatgga
     4921 ccgattgaca agtaaagaac tgagaggtaa agagtcgcaa accaaggcgc tatcttcgtt
     4981 gaaatctcgt agatatatcc tttaggattt agcgttccaa taataagggt caagacggcg
     5041 ataccaacgc ctgaaaagac aaaacctgcg atggcaggaa gaaaatgttc tccagataga
     5101 gcacctagag aaggcggaaa aatcaagttc cccgcaccaa aaaatattcc aaacaggagc
     5161 aaacctgtta gggtaccttt tttagccata aaaatctcct tcatatttat aaaatactga
     5221 tctagtatac cacgattagg agtctgaaaa aagcatcacg aggcaaatat taaatgtttt
     5281 caattttctt aaaaataaga attatctaaa aataaaactc tcctacccag tccacaccaa
     5341 gtaggagaaa tgcaaaattt taaagttctt caaaggctaa catgccaccc tggacattga
     5401 taacattata accttgttct aataggaatt ggcaagcacg cgccgatctc attccagatt
     5461 tgcaaataat ataatgcaag cgatctttgt ccaattaatc ataactatca gccaattgac
     5521 taagcggtag gttgtgggca ccttctaaat gaagagctgc aaactcatcc acttctctca
     5581 catccactag agaaagctgg tcgttttggt aaagctggta aaatgcgtca aaggctattt
     5641 ctttcattct ttttcttcta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc
     5701 ttaccgtgcg tatgttaccg acttcgtcag ttttatctgc aacttcaaag ctgtactttg
     5761 agcagcctac g
//
LOCUS       DAVBCG010000117         5760 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBCG010000117 DAVBCG010000000
VERSION     DAVBCG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.117"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(52..1113)
                     /locus_tag="VQ495_001726"
     CDS             complement(52..1113)
                     /locus_tag="VQ495_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2531655.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ495_001727"
     CDS             complement(1106..3937)
                     /gene="uvrA"
                     /locus_tag="VQ495_001727"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2531656.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILSPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDNSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPNASKTLHEGALAPWNPISSNYYPNMLEQAMTAFGVAMDKP
                     FEDLSEEDKNLILYGSDGKKFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGEQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            4069..5013
                     /locus_tag="VQ495_001728"
     CDS             4069..5013
                     /locus_tag="VQ495_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2531657.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5025..5708
                     /locus_tag="VQ495_001729"
     CDS             5025..5708
                     /locus_tag="VQ495_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2531658.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASVFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
BASE COUNT         1597 a         1401 c         1170 g         1592 t
ORIGIN      
        1 aatttttttt gacttttttc gtcattttcg tacttttttc ttgacaaaca actaaatgac
       61 tatcaactct tttggagcta gggtcaataa ttcacaacct gtctctgtaa tcaggatatc
      121 atcctcgata cgaacgccat atttgccttc gatatagata cctggttcat cggtcaaggc
      181 catacctgtc ttaatagttt ctgtagaagt ctgactaaag tagggttcct catggatatc
      241 cagaccaata ccgtggccaa tgccgtgagt aaagtagtca ccataacctg cctcaatgat
      301 aatatcacga gggattttgt caaagtcacg gaaacctaag cctgccttag cttggtcaat
      361 caaggcttgg ttagctttta gaaccgtatt gtaaatctct gcctgctcat cgctaacatg
      421 ccctagatag atagtccggg tcatatcact gacatagtgg tcatagagac agccgaagtc
      481 catggtaatg gcttctccca actccactgg tttgtgcatt ggatgggcat ggggtttaga
      541 agaattgata ccgctagcta ggatcgtatc aaaagataag ccagatgctc ccaactcacg
      601 catgcggaaa tcaaggaagt tggcaatctc aatttcagtt tttcctggtt tgataaagtc
      661 aagcgcatcg cggaaagctt ggtctgagat agaacaagcc ttgcgaatcg ctgcaatctc
      721 tgcctcatcc ttaatcatac gaagaccttc cacaaactga gtttgtggaa gcaagttcaa
      781 acctgcaaaa gctgcctgca tacggtggta ataagacact gaaatctcat cttcaaaacc
      841 gatacgagtc aagcccatgt ccttaacaat tcctgcaatg acagccaatt catcacgatc
      901 agccacaatc tcaaaaccac tggtttcttg cttagctgcg atgatatagc gagagtctgt
      961 cactaatacc tgacggtcac ggctgataaa gactgttccg tttgagcccc aaaaaccagt
     1021 caaataatag acgtttttaa gattgttgat gatgacacca tctagttctt tttcttgcat
     1081 tttagctaga aatgcttgta cgcgtttatt catgatgtaa ctttcctttc aaatagtgtc
     1141 ctgtatagct ggcttcgttg gcagctactt cttctggagt tcctgttacg atgatggttc
     1201 caccaccgac accgccctca ggtcccaagt caatgatatg gtctgccgtc ttgataacat
     1261 ccagattgtg ctcgatgacg aggactgtat tgccatcgtc tacaaagcga gctaaaacct
     1321 tgagcaggcg agcaatgtcc tctgtatgaa gccctgtcgt cggttcatcc agaatgtaga
     1381 aagattttcc tgtcgagcgt ttgtggagtt cgctagctaa cttcatacgt tgggcttctc
     1441 ccccagaaag ggtggtagct ggctgtccca aggtcacata gcctagccct acatccttga
     1501 tggtctggag tttgcgttga attttcggaa tgtgttggaa aaattctacc gcatcgttga
     1561 ccgtcatatc caagacctgc gaaatattct tttccttgta gtgaacttct agggtttcac
     1621 tgttatagcg ggttccgtgg caaacttcac aagccacata aacatctggc aagaagtgca
     1681 tctcaatctt gataatcccg tcacctgagc aagcttcaca gcgacctccc ttgacgttga
     1741 aactgaagcg tcccttcttg tagcctcgaa tcttggcttc atttgtctga gcaaaaaggt
     1801 cacgtatatc gtcaaaaact cctgtatagg tagctgggtt agacctcggc gtccgtccga
     1861 tagggctctg gtcaatatca atcaaacggt cgacatgctc aatccctgta atagtcttaa
     1921 acttaccagg tttgtctgaa ttacggttga gcttctgggc aatggctttt ttgagaatgc
     1981 tgttgattag agtcgatttc cctgaacccg acacacctgt cactgcgata aattttccta
     2041 gtggaaagcg agccgtgaca ttttgcaagt tgttctcacg cgctcctatc acttcaataa
     2101 aacgaccatt tccgacacgg cgctcttctg gtactgggat gacacgtttg cctgacaagt
     2161 actgacctgt gatagacttg ctgttgcgag ccacttgctt aggtgtacct gctgcaacaa
     2221 tctcaccacc aaaaacaccg gcaccaggac caacgtcaat cagataatca gcttcacgca
     2281 tggtatcttc gtcgtgttcc accacgataa gagtattgcc caagtcacgc atctttttca
     2341 gactggcaat caggcggtca ttgtctctct ggtgaagacc gattgacggc tcgtctagga
     2401 tatagaggac gcctgatagg ttggaaccaa tctgggttgc caaacgaatg cgctgacttt
     2461 ccccacctga aagggttcct gctgaacgtg acagggttag atagttaaga cccacattat
     2521 taaggaaggt caaacgatcc ttgatttcct tgagaatggg acgagcaatg atggcttcat
     2581 tttcagacaa agttaactgg ctcaccaagt ccaagtgatc tgcgatagac aggtctgaaa
     2641 tttctccgat atgtggccct tgctcaccac ccacacggac agacaaggcc tggtcattga
     2701 gacgataacc gtgacaggtt ccgcaagtca gctcattcat gtagaggcgc atctgagtgc
     2761 gagtgtaatc gctattggtt tcatggtaac gacgtttgat attattgata actccctcaa
     2821 acggaatgtc gatatcgcgc acgccaccaa attcattctc atagtggaaa tggaatttct
     2881 taccatctga gccgtagaga atcaagttct tgtcttcttc tgacaagtcc tcaaaaggct
     2941 tatccatagc cactccaaaa gccgtcatgg cctgctctag catgtttgga tagtagttgg
     3001 atgagatagg attccaaggt gccagcgctc cctcatgtaa ggttttgctg gcatttggca
     3061 ctaccaaatc agtatccacc tccagcttga tgcccaagcc gtcacactca ctacaagagc
     3121 caaaaggagc attgaaagaa aagagacgag gctctaactc tgggacagta aaaccacaaa
     3181 ctggacaggc ataatgctca gagaacaaca actcagagtt gtccatggtg tcgataatga
     3241 cataaccttc tgcaatacga agggcagcct caatggaatc aaagagacga ctacgaatgc
     3301 cctccttgat aacaatacgg tcaaccacga catcgatatt gtgttgcttg ctcttagaca
     3361 actctggcac ttcggtcaca tcatagactt ccccatccac acggacacga acatacccgt
     3421 ctttctgaac cttctcgata acactcttat gttggccttt tttcttgcgg atgacaggag
     3481 acaagatctg caagcgctgg cgttcaggta actccaaaac cttatcaacg atttgctcca
     3541 cagaagaagc cttgatagcc ccatgcccgt tgatacagta aggcgtcccc acacgcgcgt
     3601 agaggagacg cagatagtca ttgatttcag tcgttgttcc caccgtcgag cgagggtttt
     3661 tgctagtcgt tttctggtcg atagaaatag ctgggctgag accatcaatg gcatctacat
     3721 ctggtttttc catatttccc aagaactgac gagcgtaggc ggacaaactc tctacatagc
     3781 gacgttgtcc ctccgcatag agggtatcaa aagccaaact ggacttccct gaacctgaca
     3841 agccagtcac gacaaccaac ttgtctcgcg gaatctccac atcaatattt tttaaattat
     3901 gggcacgcgc cccatgaatg acaattttat cttgcatctt tgttctttct agtccattat
     3961 tgcttaccat tataccaaaa aaagtgagat tctattaccc aaaaggccga ttttgtagta
     4021 taatagtaca gtgtgaaaaa atctgaaaaa tgagaaagga taagggatat gaaacaagtt
     4081 tttctctcta caacaactga atttaaagag atcgatacgc ttgaaccggg tacttggatc
     4141 aatctcgtca atccgactca aaatgaatca ctcgaaatcg ccaacacctt cgatattgat
     4201 attgccgacc ttcgagcacc gctcgatgcg gaagaaatgt ctcgtattac cattgaagac
     4261 gagtataccc tgattatcgt agacgtgccg gtcacggagg aaagaaataa ccgcacctac
     4321 tacgtaacca tcccgcttgg tattatcatc actgaggaaa ccattatcac tacgtgtttg
     4381 gaaccactac ctgtccttga tgtctttatc aaccgtcgat tgcgtaattt ctataccttc
     4441 atgcgttcac gttttatctt tcaaattctt tatcgcaatg cagagcttta cctaacagcc
     4501 cttcgttcaa tcgaccgcaa gagtgaacaa atcgaaagtc aactgcatca atcaactcgt
     4561 aatgaagaat tgattgagct catggaattg gaaaaaacta tcgtctattt caaggcctcc
     4621 ctcaaaacaa atgagcgcgt gattaagaaa ttgaccagtt caaccagcaa tatcaagaaa
     4681 taccttgagg acgaagacct gcttgaagac accctgattg aaacccaaca ggccatcgag
     4741 atggcagata tttatggaaa cgtcttgcat tctatgacag agacctttgc ctctatcatt
     4801 tctaacaacc agaacaacat catgaaaacc ttggcccttg tgaccatcgt catgtccatc
     4861 ccaaccatgg tcttttctgc ctacgggatg aactttaagg ataatgaaat tcccctaaac
     4921 ggagagccaa atgccttctg gttaatcgtc tttatcgcct ttgctatgag tgtctcgctc
     4981 actctctatc tcatccataa aaaatggttc taaaaggagt tcctatgtct caaattgatc
     5041 tacaaaaatt aactaagaaa aaccaagagt ttgtccacat tgctacccaa caattcatca
     5101 aagatgggaa aacagacgct gaaatccaga ctatttttga ggaagtcatt ccccaaatcc
     5161 ttgaggagca atctaaaggt acaactgccc gttccctata cggcgcacca actcattggg
     5221 ctcatagctt cactgtcaaa gagcagtacg aaaaagagca tccaaaagaa aatgatgacc
     5281 caaaactgat gattatggac tcagctcttt tcatcactag cctctttgcc cttgtcagcg
     5341 ccctcacaac cttctttgcg gcagatcaag ctttcggcta tggattgatt actcttctat
     5401 tagttggact ggttggtgga tttgccttct acttgatgta ctactttgtt taccaatact
     5461 atggaccaga tatggatcgc agtcaacgtc cacctttctg gaaatctgta ctagttatcc
     5521 tagcttctgt gttcctttgg ttgcttgtct tctttgcaac aagcttccta ccagctagcc
     5581 ttaacccagt actggatcca ttgccactag ctattattgg agcagccctc ctagcccttc
     5641 gcttctatct caagaaacgc ttgaatatcc gtagtgcaag tgcaggacca acacgctatc
     5701 aagaataaga aaacgataaa agcaactgca ggtgcggttg ctttttcact tacttttttg
//
LOCUS       DAVBCG010000118         5648 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBCG010000118 DAVBCG010000000
VERSION     DAVBCG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.118"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            153..1172
                     /gene="galE"
                     /locus_tag="VQ495_001730"
     CDS             153..1172
                     /gene="galE"
                     /locus_tag="VQ495_001730"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2531659.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAIEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1174..2127
                     /locus_tag="VQ495_001731"
     CDS             1174..2127
                     /locus_tag="VQ495_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2531660.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRKLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDDRLKKIEFI"
     gene            2279..2944
                     /locus_tag="VQ495_001732"
     CDS             2279..2944
                     /locus_tag="VQ495_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531661.1"
                     /translation="MLSKEDYTEEIGLIKKQNYVEAELYPIVADIIKPTLKDSLSKRY
                     VFGRQRRGLGQIYYGLSNFPDIVILDKTYENKSRKSIKIEEWKKLRGCVEVKNLNYSL
                     ITEEKIKSTISNSFEHITGEMEQLTGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKE
                     EIDNTIVEVVNKRIETEEAGNSFDWWKNIKDLSFNYTDICLSKDCRQEWNEFVKRVKE
                     IEW"
     gene            2983..3084
                     /locus_tag="VQ495_001733"
                     /pseudo
     CDS             2983..3084
                     /locus_tag="VQ495_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(3135..3341)
                     /locus_tag="VQ495_001734"
     CDS             complement(3135..3341)
                     /locus_tag="VQ495_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2531662.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            3393..3869
                     /locus_tag="VQ495_001735"
     CDS             3393..3869
                     /locus_tag="VQ495_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2531663.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            3878..4549
                     /locus_tag="VQ495_001736"
     CDS             3878..4549
                     /locus_tag="VQ495_001736"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2531664.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDFETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            4680..5018
                     /locus_tag="VQ495_001737"
     CDS             4680..5018
                     /locus_tag="VQ495_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2531665.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            5021..>5648
                     /locus_tag="VQ495_001738"
     CDS             5021..>5648
                     /locus_tag="VQ495_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2531666.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
BASE COUNT         1768 a          945 c         1254 g         1681 t
ORIGIN      
        1 atggcaaggc gacgttgacg tggtttgaat tggattttcg aagagtataa gcattttact
       61 gaaattttat caggtagttt aggatggcaa atgacattcc ctgtcaaaaa tggtaaaata
      121 agaaaaaata atctgagaat cgaggaaaaa agatgcaaga aaagattttg gtaactggtg
      181 gtgccggttt tattggaacc cacactgtta ttgaattgat ccaagcaggc catcaagttg
      241 ttgtggtgga taatcttgtc aatagcaatc gtaagagttt agaagttgtt gaaagaatca
      301 caggagttga gattcctttc tatgaggcag atattcgtga cacagctacc ctcagagata
      361 ttttcaagca ggaagaaccg actggtgtca ttcactttgc tggtttgaag gctgttggcg
      421 aatcaacacg tatccctctt gcctactatg acaacaatat cgctggaact gtcagccttt
      481 tgaaagccat agaagaaaac aactgtaaaa acatcatctt cagttcttct gcgacagttt
      541 acggggatcc gcacacagtg cccatcttgg aagatttccc actttcagtg accaacccat
      601 acggtcgtac taagctcatg ctagaggaaa ttttgactga tatttacaaa gcagactcag
      661 aatggaatgt tgtcttgctt cgttacttta acccaatcgg agcccatgag agtggtgatt
      721 tgggagaaaa tccaaacggt attccaaaca atctcttgcc atatgtgact caagtagccg
      781 ttggaaaatt agagcaagtg caagtgtttg gagacgatta cgatacggaa gatggaacag
      841 gtgttcgtga ctatatccac gttgtcgatt tggctaaggg tcacgttgca gctttgaaaa
      901 aaatccaaaa aggttcagga ctaaacgttt ataaccttgg aactggtaaa ggttactcag
      961 ttcttgaaat tatccaaaac atggaaaaag cggtgggacg tcctattcct taccgtatcg
     1021 tagaacgtcg cccaggtgat atcgctgcct gctactcaga cccagcaaaa gctaaagcag
     1081 aactcggttg ggaagcagaa ctcgacatca cccaaatgtg tgaagacgca tggcgttggc
     1141 aaagcaagca tccaaatgga tttgaagact aagatgatga tttcaatcat cgtcccttgt
     1201 ttaaacgaag aggaagtact tcctcttttt tatcaggctc tggaagcttt acttccagat
     1261 ttggaaacag aaatcgagta tgtctttgtc gatgatggat caagtgatgg gaccttggaa
     1321 ctcttaaagg cctatcggga gcaaaatccg gcagtccatt atatttcttt ctctcgaaat
     1381 tttggcaaag aagcagccct ttatgcaggc ttgcaatatg cgacaggaga tttggtggtg
     1441 gtgatggatg cagacctcca agatcctcct agtatgttgt ttgagatgaa aaatgtacta
     1501 gacaaaaatg tagacttgga ctgcgttggg acacggagaa ctagtcggga gggagaaccc
     1561 ttctttcgca gtttctgtgc tgttctcttt tatcgcctca tgcaaaaaat tagcccagtg
     1621 gctctgccgt cgggtgtccg tgattttcgt atgatgagaa ggtctgtagt cgatgccatt
     1681 ttaagcttga ccgagtccaa tcgtttttca aaggggctct tcgcctgggt gggtttcaaa
     1741 acctactatt tggactatcc aaatgtcgaa aggcaggctg gcaagaccag ttggagtttt
     1801 aggaaactct ttttctactc cattgaaggg attattaatt tttcagattt tcccttgatt
     1861 atagcctttg tagctggtct cctatcttgt tttctttctc tgctgatgac cttttttgtt
     1921 gtggttcgga ccctcatttt gggcaatccg acatctggtt ggacctctct gatggctgtt
     1981 attctctttc ttggaggcat tcaactcttg accattggga ttctcggcaa gtatatcagt
     2041 aagatttatt tagagactaa aaaaagacca ctttatctta tcaaagaaaa aagtgatgat
     2101 aggctaaaaa agatagaatt tatctgacat agtcactttt tgttatttgt aattctttta
     2161 gttcggagtt tgggtgattt ttaaaagaat tagggatttg aaatgattta aaaaatctat
     2221 gtagactaaa tttaaattgt agtgacttaa taaataaaaa atttaggagt attatgttat
     2281 gctaagtaaa gaggattata ctgaagaaat cgggctaata aagaaacaaa attatgtaga
     2341 ggctgaattg tatcctatag ttgctgatat aattaagcca actttaaaag atagtttatc
     2401 aaaaagatat gtcttcggaa gacaaagaag gggtttggga caaatttatt atggtctaag
     2461 taattttcct gatatagtta ttttagataa gacttatgaa aacaaatcta ggaaaagtat
     2521 aaaaattgaa gaatggaaga aacttcgagg atgcgtggaa gttaagaatt taaattatag
     2581 tttaattaca gaggaaaaaa tcaaatcaac catatcgaac agttttgaac atataactgg
     2641 agagatggaa caactaactg gagagatggg acaacttatt ggagaccttt tatggtataa
     2701 aaaagtaatt tatacaaatg gaattgaatg gagatttcta agtttagatg acaaggaaga
     2761 gatagataat acaattgttg aagtagttaa caaaagaata gaaacagaag aagcgggaaa
     2821 ttcttttgat tggtggaaga atattaaaga tttatcgttc aattatacgg atatatgcct
     2881 atctaaagat tgtagacaag agtggaatga atttgtaaaa agggtaaagg aaatagaatg
     2941 gtagaagtct attctcattg agaaatttag attttaagtt acagtaagat ttatttagag
     3001 actaaaaaaa gaccacttta tcttatcaaa gaaaaaagtg accttcctga ttttacagaa
     3061 aaaaataaag tgaaaagact ataattttac atggaaatgt gctaaactag agggagtgga
     3121 ttatcgccat tgatttaggc ttcctggttt ccaatcaggg cgcgagttgg gcaattttta
     3181 acagcctcta agacatcctg actaggagaa atttcttttt ccagttggtc agggtcatcg
     3241 taaaaacgca cgattccatt atcgtggtaa tcaaataaat cagaataagt ttggcaaagc
     3301 ccacaggcga tacatcgttc aggtataagt gtgattttca tgttcatatt tatattgtaa
     3361 taagaaaagt aaaaaaaaac aaggagtaag gtatggcaaa agaaccgtgg caagaagata
     3421 tctatgatca agaagaatca agagcagagc gtcggcatcg aaaccacgga ggggctgata
     3481 ggatggctaa tcgtattttg acgatcctag ctagtatttt ctttgtaatt gtggtggtga
     3541 tggtcatcgt tctcatctat ctatcatcgg ggggaagtaa tcgcacagca gccttaaaag
     3601 actttcatga ttctgatgca agtgtagtac aaatctcatc ttcaagcagt tctcagcctg
     3661 agcagagttc agagccagaa tctacttcta gtagttcaga agaagctgct aatcctgaag
     3721 gaacgattaa agttctcgca ggagaagggg aagcagctat tgccgctcgt gcaggaatct
     3781 ccattgctca gttagaggcc ttgaatcctg ggcacatggc tacaggatct tggtttgcta
     3841 atccaggtga tgttataaaa ataaaatagg agccgatatg aaaacaattc aaattgctat
     3901 tgatggtcct gcttccagcg gtaagagtac ggtcgcaaag attattgcta aggattttgg
     3961 attcacctac cttgatacag gagctatgta tcgtgcagcg acctatatgg ctcttaagaa
     4021 ccaattagga gttgaagaag tcgaagccct tctagccttg ttggaccagc atccaatcag
     4081 ctttggacgt tcagaaactg gagaccagct tgtttttgta ggagatgtgg atattaccca
     4141 tcctatccgt gaaaatgaag tgaccaatca tgtttctgct attgcagcaa ttcctgaagt
     4201 gcgtgagaaa ctggtttctc tccaacaaga aattgcccag caaggcggga ttgtcatgga
     4261 tggtcgcgat attggaactg ttgtattgcc acaagcagaa ttgaaaattt tcctagtagc
     4321 ttctgttgat gagagagcag agcgtcgtta caaggaaaat attgccaagg gaattgaaac
     4381 agactttgaa accctaaaaa aggaaattgc tgcgcgtgac tacaaggata gtcatcgtga
     4441 gacttctcct ctcaaacaag cagaggatgc tgtctacctt gatacaactg gtttgaacat
     4501 tcaagaagta gttgaaaaaa tcaaagcaga agctgaaaaa agaatgtaaa tgtagaaaag
     4561 ccgatagatt gatatcggtt tttttctaaa tttgtcaaaa tgctgatttt agggtataat
     4621 agttgctgtt cgagaaattt tgattttcaa agaatagtga atgatgataa ggagaaacca
     4681 tgaacaattt accaaattgt ccaaaatgta actcagagta tgtctacgaa gacggtgccc
     4741 tactggtttg cccagagtgt gctcatgagt ggaatcctgc tgaagttgca gaagtagaag
     4801 aaggtcttgt cgctatcgat gccaacggaa ataaattggc tgatggtgat acagtaactc
     4861 tcatcaagga cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg acgcgcgtga
     4921 aaaatatccg catcgtagaa ggcgaccaca atatcgactg taagattgat ggttttggtg
     4981 ccatgaaact taaatcagag tttgtgagga agatttaagc atgtcaaaac actataaact
     5041 tgtattttat agccgtatct tcttgtttct agcggctttt acgggagttt atcttgaaat
     5101 tactaagcat ggtggttttg ggatgcttct ctattacacg gttctgtcca acctcttggt
     5161 aactattttt accctttatc ttctaaaggt tatgagccgt gtaggtgaaa attggcaaag
     5221 gccaagtctc ttgcgcttaa aaggtggggt caccatgagt atcatgatta cctgtgtgat
     5281 ttaccatttc ctcttggcgc ccattgcgac taatttctat accctagaaa atttcctttg
     5341 ccactatatc gttcccatct ggtttttagc ggataccctc ttttttgaca aacagggtca
     5401 atacaagatt tgggatccag cagtgtggac cattttaccc tttctgtata tgatgtttgc
     5461 tctttttaat ggcttggttc taaaactcaa tattccaaat gccaaggata atcctttccc
     5521 ttacttcttt ttgaatgtga acaagggttg gaatgttgtg tttaagtggt gtctgattat
     5581 ctttgttgcc tatatggtag caggatttat tttctacttt atcaagcaaa tcaagagaaa
     5641 gtcatcct
//
LOCUS       DAVBCG010000119         5635 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBCG010000119 DAVBCG010000000
VERSION     DAVBCG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.119"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..1958)
                     /gene="nrdE"
                     /locus_tag="VQ495_001739"
     CDS             complement(<1..1958)
                     /gene="nrdE"
                     /locus_tag="VQ495_001739"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2531667.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGL"
     gene            complement(2039..2257)
                     /gene="nrdH"
                     /locus_tag="VQ495_001740"
     CDS             complement(2039..2257)
                     /gene="nrdH"
                     /locus_tag="VQ495_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2531668.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            2608..2871
                     /locus_tag="VQ495_001741"
     CDS             2608..2871
                     /locus_tag="VQ495_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2531669.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            2877..4610
                     /gene="ptsP"
                     /locus_tag="VQ495_001742"
     CDS             2877..4610
                     /gene="ptsP"
                     /locus_tag="VQ495_001742"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2531670.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQVAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            4865..>5635
                     /gene="phtA"
                     /locus_tag="VQ495_001743"
     CDS             4865..>5635
                     /gene="phtA"
                     /locus_tag="VQ495_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2531671.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNW"
BASE COUNT         1776 a         1044 c         1213 g         1602 t
ORIGIN      
        1 agtccttgat ccacgtgttc agtcgcagca gcgtaaacat cgatgacttt acgcatatcc
       61 atatcgtagg cagaagtgta gtaaggaatg gtttctgtag acaagccagc agcagggtag
      121 tagattttac caattttctt ctcttggcgt tcttcgatac gttgcgtaat cgggtggata
      181 gaagcagaaa cgtcgttgat atagctgata gaaccatttg gcgctacagc aaggcgattt
      241 tggtggtaaa gaccatcttc ttgaaccttg tcgcgaagtt cagcccaatc agcaacacca
      301 gggataaaga catttttgaa gagttcttta acacggtctg atgttggaac aaattcacca
      361 gttacatact tgtcaaagta acttccgtta gcatagtctg atttttcaaa gttgtggaag
      421 gtaataccac gttcacgcgc aatattgttt gactctacca aggtccagta gttcataagc
      481 ataaagtaga tgcttgtaaa ttcaacagac tcaggtgaac catattcaat gagttgttgg
      541 gcaaggtagc tgtgcagtcc catggcaccg agaccaaagg tgtgggcttg gctatttcca
      601 tggtcaatcg ttggtacagc tacgatatgt gaactatctg taacgaaagt aagggcacga
      661 accatagcac ggatagaacg accaaaatca ggtgaagtca tcatgttaac cacgttggtt
      721 gaacccaggt tacatgaaac atctgttccc atttgaagga attcttgagc atcgttgatc
      781 aagcttggtt cttgaacttg aagaatctca gaacacaagt tactcatgat aatctttcca
      841 tcaacaggat ttgcacggtt agccgtatcg atgttgacta cataaggata gccagactct
      901 tgttgcaatt tagagatttc agtttccaaa tcccgcgcct tgatttttgt cttgcgaata
      961 tttggatttg cgaccaattc atcgtatttt tcagtaatgt cgatgtaatt gaatggcaca
     1021 ccgtattctt tttctacaga gtaagggctg aagaggtaca tttcttcatt tttacgagcc
     1081 aattcgtaga atttatcagg tactacaaca ccaagtgata gagtcttgac acgtactttt
     1141 tcatcagcgt tttctttctt agttgaaagg aaagcgatga tatctgggtg aaagacgttg
     1201 aggtagacaa caccggcacc ttgacgttgc cccaattggt tggagtaaga gaagctgtct
     1261 tcaaaaagct tcataacagg aacgacacct gaagcagctc cttcatagcc tttgataggt
     1321 gcaccagctt cacgaaggtt gctgagggta attcccacac caccaccaat acgtgaaagt
     1381 tgaagagctg agttgataga acgtccgata gagttcatat catccgtcac ttggattagg
     1441 aaacaagata ccaactctcc acggcgagca cgtccagcat tcaagaagga aggagtagca
     1501 ggttggtagc gttggtggat gatttcattg gcaatatcga ttgcaacagc ttcattccca
     1561 tcagcgaaat aaagggcgtt gaagaagaca cggtcttcca tattttcaag atagtattca
     1621 ccgtcattag tctttaaggc atattgattg taaaatttat aagctgccat gaatgacttg
     1681 aattggaagt tttggtcttt gataaattga gctaattctt ccaagaactc tggacggtat
     1741 ttcttgataa aggctgtttc gatgtagttg tgttcaatga ggtaattgat tttgtctttg
     1801 attgaatcaa aaaccatagt gtttggaact acattttctt taaagaaagc atccaaggct
     1861 tccttgtctt tatgaagcat gatttgtcca ttaacaggac ggttaatttc gttattaaga
     1921 cggaagtaag tcacgtcttc aagatgtttt aatcccataa aatttccctt atctaattac
     1981 aaaagaaagg cttctaagtt agccctaaaa gcagtttctt ctggatgatg tactaagatt
     2041 atgctaattg tttcagtttt cctggttgga aacctgaaaa gacttcagtt ggtgtttgga
     2101 taacaggagc tgcgctaaaa ccgagctctt taacttgatc gacgtactca ggttgctcat
     2161 caagattgat ttcacgataa gagacattat tactgtccaa gaaacgcttg gtcattttac
     2221 attggacaca attgttttta gaataaacgg ttaccattgt gtaactcctc ttcaaaattt
     2281 aatactatct tagtatatca gaaaataaaa ttttgtcaat gatttgaact aaatatagtg
     2341 gtgttctttt gttgttttta tatataaaca caatatatag tgtttgtatt gtgaaatagc
     2401 aatgattatc ctataaattg tttttaaaga aactatatcc tgtgttttgt tatctaggat
     2461 agaagatttt cagcaagtta tctgaaagga aattgaagga aaatccagaa aatgcttgaa
     2521 aaaaatccta gaagatggta taatactaaa ttgtaagggt tatcacatat aactcaaaaa
     2581 aagaaagaac aaaaggagag tcaaactatg gcttctaaag atttccacgt agtggcagaa
     2641 acaggtattc acgcacgtcc agcaacattg ttggtacaaa ctgctagcaa atttgcttca
     2701 gatatcactc ttgagtacaa aggtaaatca gttaacctta aatcaattat gggtgttatg
     2761 agtcttggtg ttggccaagg tgctgacgta actatctcag ctgaaggtgc agatgcagat
     2821 gacgctatcg ctgcaatctc agaaacaatg gaaaaagaag gattggcata agggaaatga
     2881 cagaaatgct taaaggaatc gcagcatctg acggtgttgc agttgcaaaa gcatatctac
     2941 tcgttcagcc ggatttgtca tttgagacta ttacagtcga agatacaaac gcagaagaag
     3001 ctcgccttga tgccgctcta caggcatcac aagacgagct ttctgttatt cgcgagaaag
     3061 cagtaggtac gctcggtgaa gaagcagctc aagtttttga tgctcactta atggttcttg
     3121 ctgacccaga aatgatcagc caaatcaagg aaactatccg tgcgaagaaa gtgaatgcag
     3181 aagcaggtct gaaagaagtt acagatatgt ttatcactat ctttgaaggc atggaagaca
     3241 acccatacat gcaagaacgc gcagcggata tccgcgacgt gacaaaacgt gtattggcaa
     3301 accttcttgg taaaaaattg ccaaacccag cttctattaa tgaagaagtg attgtgattg
     3361 cgcatgactt gacaccatct gatacagctc aattggacaa aaactttgta aaagcttttg
     3421 taaccaacat tggtggacgt acaagccact cagctatcat ggcacgtaca cttgaaattg
     3481 ctgctgtatt aggtacaaat aacatcactg aaatcgttaa agacggtgac atccttgctg
     3541 ttaacgggat cactggagaa gtgattatca acccaacaga tgaacaagtg gcagaattta
     3601 aagcagctgg tgaagcctat gcgaaacaaa aagctgaatg ggcacttttg aaagatgctc
     3661 aaacagtgac tgctgacggt aaacacttcg agttggctgc caatattggt actccaaaag
     3721 acgttgaagg tgttaacaac aacggtgcag aagctgttgg actttaccgt acagagttct
     3781 tgtacatgga ttctcaagac ttcccaactg aagatgagca gtatgaagca tacaaggctg
     3841 ttcttgaagg aatgaacggt aaacctgttg tcgttcgtac aatggatatc ggtggagata
     3901 aggaacttcc ttacttcgat atgcctcacg aaatgaaccc attccttgga ttccgtgctc
     3961 ttcgtatctc tatctctgag actggagatg ctatgttccg cacacaaatc cgtgctcttc
     4021 ttcgtgcgtc tgttcacggt caattgcgta tcatgttccc aatggttgcg ctcttgaaag
     4081 aattccgtgc agcgaaagca gtctttgatg aagaaaaagc aaaccttctt gctgaaggtg
     4141 ttgcagttgc ggataacatc caagttggta tcatgatcga gattcctgca gcggctatgc
     4201 ttgcagacca atttgctaaa gaagttgact tcttctcaat tggtacaaac gacttgatcc
     4261 aatatacaat ggcagcagac cgtatgaacg aacaagtttc atacctttac caaccataca
     4321 acccatcaat cctacgcttg attaacaatg tgatcaaagc agctcacgct gaaggtaaat
     4381 gggctggtat gtgtggtgag atggctggtg accaacaagc tgttccactt cttgtcggaa
     4441 tgggcttgga tgagttctct atgtcagcaa catctgtact tcgtacacgc agcttgatga
     4501 agaaactcga cacagctaag atggaagagt acgcaaaccg tgcccttaca gaatgctcaa
     4561 caatggaaga agttcttgaa cttcaaaaag aatacgttaa ttttgattaa tcgaaaagtc
     4621 cctgcaactc agttacaggg actttttttg atattttaaa aagaattttc aagaaaatct
     4681 ttcttataaa aagtccaacc ttgaaaaagt agtggtcaga acaaaaaata cttaaatggt
     4741 tcataaaatt cttgacaagt tggagattta ggagtaaact attaaccagt taagtaatag
     4801 agaggagttt ctgcaattta gaaatgaatt gcaactagaa atatcaaata gaaagagagt
     4861 ttcgatgaaa attaataaga aataccttgt tggttctgcg gcagctttga ttttaagtgt
     4921 ttgttcttac gagttgggac tgtatcaagc tagaacggtt aaggaaaata atcgtgtttc
     4981 ctatatagat ggaaaacaag cgacgcaaaa aacggagaat ttgactcctg atgaggttag
     5041 caagcgtgaa ggaatcaatg ctgagcaaat cgtcatcaag ataacagacc aaggctatgt
     5101 cacttcacat ggcgaccact atcattatta caatggtaag gttccttatg atgcgatttt
     5161 cagtgaagaa ttactcatga aagatccaaa ctataagcta aaagatgagg atattgttaa
     5221 tgaggtcaag ggtggatatg ttatcaaggt agatggaaaa tactatgttt accttaagga
     5281 tgctgcccac gcggataacg tccgtacaaa agaggaaatc aatcgacaaa aacaagaaca
     5341 tagtcaacat cgtgaaggtg ggactccaag aaacgatggt gctgttgcct tggcacgttc
     5401 gcaaggacgc tatactacag atgatggtta tatctttaat gcttctgata tcatagagga
     5461 tactggtgat gcttatatcg ttcctcatgg agatcattac cattacattc ctaagaatga
     5521 gttatcagct agcgagttgg ctgctgcaga agccttccta tctggtcggg gaaatctgtc
     5581 aaattcaaga acctatcgcc gacaaaatag cgataacact tcaagaacaa actgg
//
LOCUS       DAVBCG010000120         5469 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBCG010000120 DAVBCG010000000
VERSION     DAVBCG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.120"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(2..196)
                     /locus_tag="VQ495_001744"
     CDS             complement(2..196)
                     /locus_tag="VQ495_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2531672.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(656..1384)
                     /locus_tag="VQ495_001745"
     CDS             complement(656..1384)
                     /locus_tag="VQ495_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2531673.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1542..2951
                     /locus_tag="VQ495_001746"
     CDS             1542..2951
                     /locus_tag="VQ495_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2531674.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2969..4264
                     /locus_tag="VQ495_001747"
     CDS             2969..4264
                     /locus_tag="VQ495_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2531675.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPSAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            4269..4577
                     /locus_tag="VQ495_001748"
     CDS             4269..4577
                     /locus_tag="VQ495_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2531676.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4574..4882
                     /locus_tag="VQ495_001749"
     CDS             4574..4882
                     /locus_tag="VQ495_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2531677.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1848 a          921 c          970 g         1730 t
ORIGIN      
        1 actatagtaa agttgacttt cctgtaccat ttttaccagt aattaatgtc acgtctgaac
       61 tcgaagtatc aaaagaaata ttatcaaaca atatacgatg atccacccta tacgaaatat
      121 tttctacttt cattttttct ccttttgaac taatagcaaa agaaacagtt ctgttttcat
      181 actctttccc cttcatctat attattcgta atgaaaaata aaaaaataaa atttttttaa
      241 atgattatga tccagaattt atttaatata ttaaaataat atataagcaa ataaattggg
      301 agattgaaat tcatttctag caatttgtta gaagtcacag tctactattc taacctcagt
      361 atactatatg tgttacaaga aaagactcaa aaacttctcg tcagtgtaag aatgtttgaa
      421 acactattta aaactaaata actataagaa gtagatcagg tgaacaattt cagtacctac
      481 tctataagct atacgaagat atttttcaac atgatggata aaacgatcac tcttttaaag
      541 ccatcttatt tagaaaagag aaaccattta agcatagatg ggaccttctt gccctgtttt
      601 aaatttttct taaactagag cctgtaaaag attggcttca ttttcacatt ttctcctagg
      661 aggaatgtcg taatgcaaaa gaattaaatt caaataaatc atagcgatga cgactaatcg
      721 agtactcaaa cggagttccg ttatccaaat aagccacttg ttctacttca aataccggtt
      781 ctgttggaag gagctgcaag taatgttgct cattttctga actagaagaa gctcgcaaga
      841 tttttgtagc actggcaata tgcaatccta actttccttc aatgtaattg taaatcgatt
      901 tttgtaaaat atcttcagta atacctggaa taacatcggt actcatataa gtttgttcca
      961 gaacataagg tttaccgtca ataatacgca ggcggtaaat attataaact ggactctgca
     1021 aagcgacctg tagtttttct gctaaaaatt catttgcaaa ttctaatttg aagtgaataa
     1081 tcctcgagtc aattgtagaa tgagcatctt cagatatttt tgtcagtccc cggatatctc
     1141 tttctggaac gattaatttt ctttttgagc tgccacgaga gagaacaaag gttccctgcc
     1201 cacgttttct aaaaattaag ccctcagaaa ctaacaagtc taaagctttt ttgatcgtca
     1261 ttcgactaca ggcaaactgt ttagctaatg tcatttcatc aggcaattgt tcgttcatgg
     1321 tatatttatt acagagaatc ttttcacgaa tctcattgta aataaataaa tattttgtac
     1381 tcatttaatc tggttctctc tataatattc taatagtagt ataacaaagt tcaagaaatg
     1441 aggcaaacac aatatatgta tcaacaattt ttaaaaatgt ctagacaaat tttaaaatta
     1501 tgctatactt tatgtatagc ataatagaaa ggaggttgag catgaaaaaa aagttagtat
     1561 ttcctaatct gttttggtgg ggagctgctt ctagcggacc tcagacagaa ggtcaatatg
     1621 gaaaagtaca tgaaaatgtg atggactact ggttcaaaac gcatccagaa gattttttcg
     1681 ataatgtcgg acctcttgta gccagtaact tttttcatac ttacaccgaa gatttccact
     1741 tgatgaagga aattggagtt aattctttcc gcacttccat ccaatggagt cgactcatca
     1801 agaatttaga gacaggtgag cctgatccaa aaggtattgc tttctacaat gccatcattg
     1861 aagaagctaa aaagaaccag atggatcttg tgatgaattt acatcatttt gatttaccag
     1921 tggaacttct tcaaaaatac ggtggttggg aaagcaaaca tgtagtggag ttattcgtga
     1981 agtttgccaa gactgctttc acatgctttg gagataaggt tcattactgg acaactttca
     2041 atgagccaat ggtcattcca gaagcaggat acttatatgc tttccattat ccaaatctaa
     2101 aaggaaaggg aaaagaggcc gtacaagtca tctataatct aaaccttgct agtgcaaaag
     2161 tgattcaact atatcgctca ttaggacttg atggaaagat tgggattatt ttaaacttga
     2221 cacctgctta tccaagaagt aattctccag aagacttaga agcaagtcga tttacagaag
     2281 acttctttaa caaagtcttc ttgaatccag ctgttaaagg aactttccca gaaagattgg
     2341 taaaacagct agagagagat ggcgtgttat ggagtcatac cgaaaaagag cttcaactga
     2401 tgaaatcaaa tacggttgat tttcttggag taaactacta ccatccaaaa cgtgttcaag
     2461 cacaagcaaa tcctgaggaa tatcagacgc cctggatgcc agaccaatac ttcaaagagt
     2521 atgaatggcc ggagcgtcgc atgaatccat atcgtggttg ggaaattttt ccgaaagcca
     2581 tttatgatat tgctatgatt gtgaaggaag aatatggtaa tatcccatgg tttatcagtg
     2641 agaacggaat gggtgttgaa aacgaagcac ggtttatcga tgaaaatgga gttatcgatg
     2701 acgtgtatcg tattgaattt tatgaagaac atttaagatg gctacataaa gccattgaag
     2761 agggaagtct ctgttttgga taccacgctt ggaccgcatt tgattgctgg tcttggaata
     2821 atgcatataa gaatcgttac ggatttatct ccgttgattt agaaacgcaa aagagaacca
     2881 tcaagagctc aggaagatgg tatcgcaaag taagtgacaa taacggtttt gaagtagaaa
     2941 ttgaggagta aagagaggag atattcagat gaaaaactca aaatttatag atcaatttgc
     3001 cacctttgct ggtaaactag ggaaccaaat tcatttaaaa accctaagag atgcattcgt
     3061 aacagtaatg ccattatata ttttggcagg tttaatcgtt cttttgaaca acacggtatt
     3121 taagtggatt ttccaagggg atacattaac aaaattccaa tattggggaa taacaattgc
     3181 aaacggtact ttaagtattt caggtatgat tattgctgta atggttggtt atttcttagc
     3241 taaaaacaga gatttcgaaa acccgtcagc agcatcaatg ctatcattag tttctttaat
     3301 tgtgatgatg ccaaatacag tttctgtagt tcctgacgga gcaaaagatg ctgtaaacat
     3361 ttcaggtgtt ctttcattca acaacacagg tacaggcgca atgttcgccg gggttatcgt
     3421 agcgattatt gcaacagaat tattcattga attatcaaac gttaaagctt tacaaatgaa
     3481 ccttggtgaa aatattccac cagctgttag tagatcattt agcgtattac ttccagtcat
     3541 gactgtcatc tccttatttg ggattgtttc agcattatta ttcaatataa ctggaatgaa
     3601 cttaatctca atcattacaa tctttattca agaaccaatt cgtcatattg gtacaagctt
     3661 aatcggggtc attattattt actctttagg aaatatgtta tggctatttg gtattcacca
     3721 agcagttatt tacagtgcca tcctagaacc attactatta attaacatta ctgaaaacat
     3781 cactgcagca aataatggac aagccattcc acacatcatc aacttatcac aaatacaaac
     3841 attcgcttta atgggtggta gtggatctac attatgttta ttaatagcaa cattcttagt
     3901 gagtcgcaat gctgtttcta aaaacgtggc taaattatct tttggacctg gtatcttcaa
     3961 tatcaatgaa ccagtattat tcggttaccc aatcgtttat aacatttcat tagctattcc
     4021 atttatcaca gttccagtcc ttggtatttt aatcagctac ttagcaacag ttacaggatt
     4081 catgagtcct gcatttatac aagttccttg gactacacca gtattcttaa atgcatggtt
     4141 agcaacagca ggggacgtga gagcagttct agttcaattc atcatctttg cacttggagt
     4201 tcttctatac attccattta tcaaagttaa tgacaaagtt gttgagcaag aaatggaagg
     4261 ttaagattat gaaacaaatt ttattagtat gtaatgcggg aatgtcgaca agtatgcttg
     4321 ttaaaaaaat gcaacaaagt gcgactgaac gtgggattga aatttctatc caagcaaaat
     4381 caatgacaga agcgaaaaag aatatccatg aagcagatgt cattctaatt gggccacaaa
     4441 ttcgttatga gttacttgct gtgaaagaaa ttgcaggaaa tatccctgta gataccattg
     4501 atatgcggga ttacggaatg atgaatggag caaaagttct ggaacaagca cttgcatgga
     4561 taggagaaat aagatgacag aagaaatgga atatatctgt tttcagctca ttgccaatag
     4621 tggagcagcc aaatcatcct ttattgaagc catccaacta gctaaggcag gaaatttaaa
     4681 agaagcgaaa attaaagttg aagaagcgga agactcctta gtagaagcgc acaagattca
     4741 ctctaatctc atacaaaaag aagctactgg agaaaaaata ggattctcct tgctatttat
     4801 gcatgcagaa gaccaaatgg cttcaacaga gattattcag ttgctatcta aagaattcat
     4861 cgatctctat caaaataaat aggaacactg tcgttcccat ctgtctttat taatgacggc
     4921 tacattgtag gaacctccaa aagttggaca aaatgattga aaggattaga tcctgcatta
     4981 cacaagatcc aatccttttc ttttactcta gtttttataa tgtgttgaat ttagaatagt
     5041 ccactgcagt ttctaaaaaa ttgctggaaa tagatgttaa gctccaattt atttgtttat
     5101 atcttatttc agtttactat actagcttat tattctagta aaaaattgaa tgagccacta
     5161 tttatttaat atgtatgatg agggaatgac tcaaaaactt cttgtcgata taagaacatc
     5221 tgagatagta ttttaaaacg aaaggactat aaaagctcat ttccttcgca cttattttat
     5281 agagatctat cattatactc tttagaaatc aaaaagcaac acaccttgtc atcgttttat
     5341 cgtaaacctg ttaatgaggg gctagtctta tctgcaatct caaatcagtg ttttgagctg
     5401 aatggcactc gttttctagt ttgctctttg attttaattg ggccttagta aatactactc
     5461 ttaaattaa
//
LOCUS       DAVBCG010000121         5405 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBCG010000121 DAVBCG010000000
VERSION     DAVBCG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.121"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(60..2114)
                     /locus_tag="VQ495_001750"
     CDS             complement(60..2114)
                     /locus_tag="VQ495_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2531678.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            complement(2126..2380)
                     /locus_tag="VQ495_001751"
     CDS             complement(2126..2380)
                     /locus_tag="VQ495_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531679.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            complement(2373..2930)
                     /locus_tag="VQ495_001752"
     CDS             complement(2373..2930)
                     /locus_tag="VQ495_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2531680.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            complement(2914..3870)
                     /locus_tag="VQ495_001753"
                     /pseudo
     CDS             complement(2914..3870)
                     /locus_tag="VQ495_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            3962..4321
                     /locus_tag="VQ495_001754"
     CDS             3962..4321
                     /locus_tag="VQ495_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2531681.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            4532..5388
                     /locus_tag="VQ495_001755"
                     /pseudo
     CDS             4532..5388
                     /locus_tag="VQ495_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1652 a         1179 c         1011 g         1563 t
ORIGIN      
        1 cacatatata atccaaggcg acgctgacgt ggtttgaaga gattttcgaa gagtattaac
       61 taatttcttc ttaccaattc caccatatca tacggcaggg tattggcagc ttccttcaag
      121 gaatagttct ctaagttatt tacattttgt cgtaatttct tggcatactt agtcgtaatc
      181 aatcgttttt cttcgtattc gaaaatcaac ttgcgctcca gataatagcc tctcagcatt
      241 tcatcgatat tgttgggttt gacacgattg ataacccgtt cgacaaaggc accactgctg
      301 ataatagctg tttctcgaag acgagactcc tgcataaaac taatcaaaga gcgtctgtag
      361 actcccttca ggttttccaa actttcaata atcatctctg tattggcaag atagagctct
      421 gcaatttggt cataatcaag agcacggaga cggctttgct ccttgtcctt ccagctacgg
      481 aaggtctttc caagagtaaa aacttcatga aggagaaaac gtaaaatcct caaggaaaca
      541 agaaaataat aggtcagtct tgaggcaagt ttacgattga ttccttgttc tatatttttc
      601 agataacgtt ggtaaactcg gtaagcacga ttgctaatgt tcccctcttc ataggcctgt
      661 tccaaaccat cactttcaat actaagaatc aagagtttca aagcagccca atcttcttga
      721 tcatcctggt tttcttggct taaaatgaga ttttcaatac gtccatgata attgtcaata
      781 gccgcataga ggggaagttt atttctggtg tcttccaact ctttttccaa ctctagcgtt
      841 acttcattca aaatggcgat atgcatgaga taatccttgc tttcttcctc ttcatcagaa
      901 agatgaggca agaccaagag acctgttaaa aagctgacaa gcgtcacacc tgcaacaagg
      961 aaaagcaaaa gaggatactc ctgttctaga ttacttggta tcaagagaat cgtagcaatc
     1021 gacaccgttc ccttaacacc tgaaaaggtc aagagaaaca tgtccttcat atacttattt
     1081 agctttttct tgaggcgtcg ggttctatag gcataatagc catagatcat aataaaacga
     1141 atgacaaaga ggacaaaggt aagggcgatg agagatagca ataaaagtag aggattatag
     1201 attggattgg tcaagatagg ttctgctatc atttccaact ccatccctaa aatcacaaag
     1261 acagaaccgt tgagcataaa ggtcactgta tgccagaccg tctcggtcac cgtatccact
     1321 tgggcttcga ggagcgtgat tttcttgaag cgacttgcct ttaaaattcc agcaactacg
     1381 acggcaataa tacctgaaac atgaacttct tctgccagaa agaaggtcac tagaggcaaa
     1441 ctcaattcta ataaaagttc actggcaata tccgttgcgc gcacacttag caagaaggta
     1501 tggaggaagc ggttggtcat ggccgttaaa aatccaatta aaaaaccgcc taggattgaa
     1561 aagatgagcg aactgctagc ttgccccaga gaaaaagctc cagttgtcca agctgtcaaa
     1621 gctacctgaa aagccaccaa accagaagca tcattcaaga gtccttcgcc cttaagaata
     1681 ttggacacgc gcttaggaaa gctaaaacgc tccgaaagag aggcaaaggc caccaagtcc
     1741 gtaggaccaa gggctgcccc aacagccaag caagctgcca agggaaggct gaaccaaaga
     1801 agatgggcca agccacccaa actcaaggtc gagataaaaa tcactggaaa tatgagataa
     1861 acaatgattc gccagtgttt taaaatagcc gtaacatctg cttcttcagc ctctcggaaa
     1921 agcaagggtc cgataaccag tgccaaaaac aactccgtat taaggtgaaa gtcagtattg
     1981 ggtaaaaaga gaccaatcac aattcccaaa agaatctgca ccaaagggag aggcaaaaag
     2041 ggcaggagct tattggttgt acttgagaca atcaaaacca gtaaaaatag gatgaggtaa
     2101 atcagtaatt ccacgcacgt cctccttaat cttttttaca acaggattca aatatctcct
     2161 tctgctcttt gattttttgg tcaatcttgg aacagtcttt gtgctcaatt tttctctggc
     2221 accgttccat ttcaagagca actaattttt tcttgatttt aagcattttt ttgctcatat
     2281 gcgcttggtc tagcacgccc accgatcgtt cgtggtgggt tgattcaaca aaattctggc
     2341 gcatggcatc cagcttttcg tgtaggtatt gtttatccat gtctgtatct ctctaatttt
     2401 tcaatcatca ctaaaaacgg cgggttgttg acttggttta aagttcggta aatggcagct
     2461 gtgtactctt gttggttcaa ctggatcaca aaatccaaga cagcatctct ctcgagatcg
     2521 cctccttcat gaccatagta aatcataata gcaattcgtc cacctttgac aagtaagcca
     2581 catagctttt ctaatgcctc aatcgttgtc tgcggtcggg tgatgacaga cttatcagct
     2641 gacggcaaat agcccagatt aaaaatccct gccttagctt ttatcacaaa ctggtccagt
     2701 gtctcatggc cttgcaagat taactgggca tttgtcaagt cagcctgatg caaacgctct
     2761 tgggtctttt ccaaggcttg cttctgaata tcaaaggcat agacttgctt ggctagcttg
     2821 gctaaaaaaa gcgtgtcatg accatttccc atagtcgcat ccactacgac atcctctttt
     2881 gtcacgacct cagccaaaaa atcatgtgcc atctcaagtg gtcttttcat tttcaaactc
     2941 ctgttttaca gccttgcatc cttgaacact tccacgacgt cgcatctcca tctcaatgct
     3001 gttgagagct tcccatttat tgaggctcca cataggccca agcagcatat ccctaggcgc
     3061 atctcctgta attcgatgga tgacgatatg tttgggaata atttccagtt ggtcacagat
     3121 gaccctgaca tattcgtcct gactcatcaa ttgtaaacgc ccctcatggt aatctcgttg
     3181 catacgagta tttgtcataa gatggagcaa atgcagttta atcccttgaa tatcgttatc
     3241 cgtgacacaa cggcggacat tttcaaccat catctcatgg gtttcaccag gcaaaccatt
     3301 gatcaaatgg gaaacaatct caatttttgg atactttctc aaacgcttga ccgtttccac
     3361 ctacaattca taagaatgcg cacggttaat caggtcagag gttgcttcat aagtagtttg
     3421 caagcccaat tcaaccgtca catgcatgca ctccgataac tcagccaaat attcgatggt
     3481 ttcgtctggt aaacagtctg ggcgcgttcc aatattgatt cctaccacac ctggctcatt
     3541 gatagcctgt tcataacgct ctcgaataac ttccaccttt tcatgggtgt tggtaaaatt
     3601 ttgaaaataa accagatact tctgaacatc tggccactta cgatgaataa agtcaatttc
     3661 cttataaaat tgctcacgga taggcgcatc tggtgccaca atggcatctc cagaaccgga
     3721 aaccgtacaa aaagtacagc ctccatgagc cacagttcca tcacgattgg gacagtcaaa
     3781 tcccgcatca atagggactt taaaagtctt ttctccaaag agttttcgat aataatcatt
     3841 caaggtatta taagatttta taactttcat tataacaaaa atcacccaca atctcaaaag
     3901 cctgactttc ctataaattc ctctgtttct cgtttccatt agcctttttt tatgatacaa
     3961 tatgggtatg attttaatga aattagcatc tattttatta ttgatactga ccttagtcgt
     4021 ctgcattatc ctaaccaaac tttttagatt aaaaaaacta ggacgaaact ttgcggattt
     4081 ggcttttcca gtcttggtat ttgagtatta cttgattaca gctaaaacct ttacccataa
     4141 tttcctccct agactggggc tagccctctc gatcctagcc attattctcg tctttttctt
     4201 ccttttgaaa aaacgcagct tttactatcc taaatttatc aaattcttct ggcgtgcagg
     4261 attcttatta acccttatca tgtatataga aatgattgtt gaattgttct taatgaaata
     4321 gtcgaatccc taagcatttt ctagggattt ttgctttctc tacaaaatag tatagacaat
     4381 aacactatac aattttatac aaagaaaaga gtctgggaca atagtctctt atctccaaaa
     4441 aaggcaacgg atttgccgtt gcttttttgc atggttgcga tagtcttggt aaaatagaat
     4501 tgcttaataa accatttaga aaggctatcc catgcatatt cactataaca caaatcaaac
     4561 aactttacca ctagaaatca gttccttctt gccacaagat catctcgttt ttactattga
     4621 aaaagtggtg aataccttgg aggattgtca cttccacgcc ttctatcatg cctttgatcg
     4681 cccgtcttat caccctaaaa tgcttgtatc tactcttcta tttgcctatt cacaagggat
     4741 tttctctggt cgaaaaattg aaaaatggaa gagttagtga ccttagattg tttgtttatt
     4801 gacggaacta agattgaagc caatgccaac aagtatagtt ttgtgtggaa gaaaacgaca
     4861 gagaaattct ccgccaaact tcaagaacag atacaggtct attttcaaga agaaatcact
     4921 ccccttctga ttaaatatgc catgtttgat aaggaacaaa agagagggta taaacagtca
     4981 gctaaaaact tagcgaattg gcactataat gacaaggagg atagctacac acatcctgat
     5041 ggctggtgct atcgttttca ccatatcaag taccagaaaa cacagacaga ctttcaacag
     5101 gaaatcaagg tttactacgc tgatgaacct gaatcagtcc ctcaaaaggg actgtatatg
     5161 aacgaacgct atcaaaactt gaaagctaaa gaatgtcagg cgcttttatc tccccaaggt
     5221 agacagattt tcgctcaacg caagattgat gtggaacctg tctttgggca gataaaggct
     5281 tgtttgggtt acaagagatg taatctgaga gggaagcgtc aagtgagaat tgacatggga
     5341 ttggtactta tggccaataa cctcctaaaa catagtgaaa tgaaataaga acaggacaaa
     5401 tcgat
//
LOCUS       DAVBCG010000122         5300 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBCG010000122 DAVBCG010000000
VERSION     DAVBCG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.122"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..987
                     /gene="ply"
                     /locus_tag="VQ495_001756"
     CDS             <1..987
                     /gene="ply"
                     /locus_tag="VQ495_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2531682.1"
                     /translation="VPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGE
                     KQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQ
                     VYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVV
                     TGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNG
                     DLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVR
                     NLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND"
     gene            1868..2584
                     /locus_tag="VQ495_001757"
     CDS             1868..2584
                     /locus_tag="VQ495_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2531683.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            2708..2938
                     /locus_tag="VQ495_001758"
     CDS             2708..2938
                     /locus_tag="VQ495_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2531684.1"
                     /translation="MDKPMLVFKRFGHQTYLMVQKEAKRCGIEFMGGPQGQVVRFWDN
                     REKNQDLVLIKDIEQELKITKSVASNLVKRIV"
     gene            3065..3179
                     /locus_tag="VQ495_001759"
                     /pseudo
     CDS             3065..3179
                     /locus_tag="VQ495_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            3196..4017
                     /locus_tag="VQ495_001760"
     CDS             3196..4017
                     /locus_tag="VQ495_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531685.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQGTNLDELWQPA
                     SMMMGMPFLAFLSVVAVGFFASRVAAFYISRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGPIMAIWAIGKILGHSEYWLWAVLVAVIVNVLMTTV
                     LMTLAFPKQSLIQGLTDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIVFMSYAM"
     gene            4024..5055
                     /locus_tag="VQ495_001761"
     CDS             4024..5055
                     /locus_tag="VQ495_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2531686.1"
                     /translation="MGFLLMGALFIVLPRTMVSAKRINQVLYLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQKVAIVGPTGTGKTTIVNLLMKFYEIDKGSIHI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGSFYADLYNS
                     QFTEDEAEE"
     gene            5121..>5300
                     /locus_tag="VQ495_001762"
     CDS             5121..>5300
                     /locus_tag="VQ495_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HET2531687.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLTCLDRDVTYGDFF"
BASE COUNT         1577 a          916 c         1242 g         1565 t
ORIGIN      
        1 gtcccagcta gaatgcagta tgaaaaaatc acggctcaca gcatggaaca actcaaggtc
       61 aagtttggtt ctgactttga aaagacaggg aattctcttg atattgattt taactctgtc
      121 cattcaggcg aaaagcagat tcagattgtt aattttaagc agatttatta tacagtcagc
      181 gtagacgctg ttaaaaatcc aggagatgtg tttcaagata ctgtaacggt agaggattta
      241 aaacagagag gaatttctgc agagcgtcct ttggtctata tttcgagtgt tgcttatggg
      301 cgccaagtct atctcaagtt ggaaaccacg agtaagagtg atgaagtaga ggctgctttt
      361 gaagctttga taaaaggagt caaggtagct cctcagacag agtggaagca gattttggac
      421 aatacagaag tgaaggcggt tattttaggg ggcgacccaa gttcgggtgc ccgagttgta
      481 acaggcaagg tggatatggt agaggacttg attcaagaag gcagtcgctt tacagcagat
      541 catccaggct tgccgatttc ctatacaact tcttttttac gtgacaatgt agttgcgacc
      601 tttcaaaata gtacagatta tgttgagact aaggttacag cttacagaaa cggagattta
      661 ctgctggatc atagtggtgc ctatgttgcc caatattata ttacttggaa tgaattatcc
      721 tatgatcatc aaggtaagga agtcttgact cctaaggctt gggacagaaa tgggcaggat
      781 ttaacggctc actttaccac tagtattcct ttaaaaggga atgttcgtaa tctctctgtc
      841 aaaattagag agtgtaccgg gcttgcctgg gaatggtggc gtacggttta tgaaaaaacc
      901 gatttgccac tagtgcgtaa gcggacgatt tctatttggg gaacaactct ctatcctcag
      961 gtagaagata aggtagaaaa tgactaggag aggagaatgc ttgcgacaaa aagaggcgat
     1021 gatctctctg cggatatctg aaagtttatc tcttgcctag cgatttccat tggagtatgc
     1081 tcgtgatgtt aaaatgaatc tatcaaacag aatgtgtaaa aaagtaaaaa gggtagccaa
     1141 ttccttgata tacaggggat tgattatcct ttacttgttt tagatgattg gaaattcaag
     1201 aacgacacgt atcggaaata ttttgctgtt tatatctaat acccatttat ggaagaaatg
     1261 aaattaaata tgaacaaatc aattgctagc aatggtttag aagtgccagt gtactagtct
     1321 aacttctcca tcttgggaac acgtaaatta tactcttcga aaatctcttc aaaccaggtc
     1381 agccctatcc gcaacctcaa aacagtgttt tgagcaacct gcggctagct ttctagtttg
     1441 ctctttgatt tttattgagt attagaggtt tgcttagaaa ctcgtctatt aatttcttag
     1501 aatacaatag cgcctataag aactgttgga ttgcaaagta tttactggaa ctatgatatg
     1561 tctggagtag actgtcaaca aaaggtgcag tatgattgtt tttgtcgctt atatctacaa
     1621 acttcaaaca gtaatttgag ctgactttgt caaacttggc tgaaactttc tatttgcaga
     1681 taatggtcta gtttgttttt tgaatgtgga tgagtgtctg ttgataaaga tagaaaggca
     1741 agaccatctt agcaggtaaa gtactatcct agcttgattt gctaataaaa tccatttcct
     1801 ctagtgaaaa tcaaaaaaac ttgtgttata ataagaaaga ttaaaatgtg aaaaaaggag
     1861 attcctaatg ggacgtaaat gggccaatat cgtagccaag aaaacggcta aagatggagc
     1921 caactctaaa gtatatgcaa aatttggtgt agaaatctat gtagcagcta aaaaaggtga
     1981 tccagatcca gaatcaaact cagctttgaa attcgttatc gaccgtgcta aacaagccca
     2041 agtgccaaaa cacgttatcg ataaagcgat tgataaagcc aaaggaaaca cagacgaaac
     2101 ctttacagaa ggacgttacg aaggttttgg gccaaatggc tctatgctaa ttgtggatac
     2161 tttgacttct aacgttaacc gtacagcagc caatgtccgt gcagcctttg gtaaaaacgg
     2221 cggaaacatg ggcgcttcag gttctgtttc ttacctcttt gacaacaaag gtgttattgt
     2281 atttggaggt gaagatgcag acgcagtctt tgagcaattg ctcgaagcgg atgtggatgt
     2341 ggatgacgta gaagcacaag aaggtacaat cacagtttac acagcgccaa ctgaccttca
     2401 caaggctatc gttgccctcc gtgagtctgg tattgaagaa ttccaagtga ctgaattgga
     2461 aatgattcct cagtcagaag tggaattgtc aggcgaagac cttgaaactt ttgaaaaact
     2521 ttacagcgtt cttgaagacg acgaagacgt ccaaaagatt tatacgaacg tagatgggtt
     2581 ttaatttaac ctatagaaca tgatcctaag tgagaaaatc acttgggatt tttttaatca
     2641 agaaaatagt tctcatagaa atttaattat tctccagaga actattttta tttagaaggg
     2701 ggagattatg gataaaccga tgttagtctt taagcgtttt ggtcatcaaa cttacctgat
     2761 ggtgcaaaag gaagccaaac gttgcggcat tgaatttatg ggtgggccgc aaggtcaggt
     2821 tgtgcgtttt tgggataatc gcgagaaaaa ccaagacttg gtcttgatta aagatatcga
     2881 gcaggaactc aaaattacca agtctgttgc tagtaatctg gttaagcgta tagtgtaaaa
     2941 tggtttggtg gaattggagg cgagtcctgt tgataagcgg gctaagtttg ttcgtctgac
     3001 ggacaaagca cgttctcaga tgcaacaggt taaggctttt tttgaacgca tagacaagca
     3061 gttgatggaa gacattgatg aagatgaatt actgattttt gagaaagttc tcggtcaact
     3121 acaggcaaaa tatcaaggga ataggaggag agaataaaga aattagccaa aagaattagt
     3181 agaaaagaat gggggatgat tttactagcc attctcttta cttgcttttc ggtctatcta
     3241 gagttggaag tgccgaccta tatctcgaaa attacggatt tgctaggtag tcaaggaact
     3301 aatttagatg agttgtggca gccggcaagc atgatgatgg gaatgccctt tcttgccttc
     3361 ttgtccgtag ttgcagttgg attttttgca tcccgagtgg cggcttttta tattagtagg
     3421 ctgagaagtg atatttttaa ccgagttttg gattactcgc agacagagat taagaaattt
     3481 tcaattccta gcctcttgac gcgtactacc aatgacatta ctcaagttca aatgttgatt
     3541 actatgggct tgcaagtggt aacgcgtggt ccaattatgg ctatctgggc tattgggaag
     3601 attttaggtc attcagaata ctggctctgg gccgtacttg tggcagtgat tgtcaacgtc
     3661 ctgatgacga ccgttttgat gacgctagcc tttccaaaac agtccttgat tcaggggctg
     3721 acagataaac tgaacagtat cactcgtgag agtttaacag gtattcgtgt cgttcgtgcc
     3781 tacaatgcag aggattatca aaatgaaaaa tttgcagcag taaatgatga attgacccgt
     3841 ttgaatttgt ttgtcaaccg tcttatggct attttgaatc ctatcatgat ggggatttca
     3901 agtggtttga gtgtggcgat ttactggatt ggggcctatg tgattaacga cgctgctccg
     3961 atagcgcgtc tgcctctctt tagtgacatg attgttttca tgtcttatgc catgtaggtt
     4021 gtcatgggct tccttctcat gggagcactc ttcatcgttc ttccccgaac tatggtctct
     4081 gctaagcgga ttaatcaagt tttatatttg cattcttcta tccaaaaccc tgttcaagtg
     4141 cagctgactg atgaaaactt caaaggtcag gtcgagttta aggatgtgac cttccgctat
     4201 gcggcaaatt cggaggcagt tattgaacat gttagcttta aagcagaaac tggtcaaaca
     4261 gtggccttta ttgggtcaac aggttctggt aaatcaactc tggtcaatct gattccacgt
     4321 ttctacgacg tgtcagcagg agaaattctg gtggacggtg tcaatgttca agactatgac
     4381 ttctctgcga cagctcatgc tggtcaaaag gttgccattg ttgggccgac tgggactggt
     4441 aagacaacca ttgtcaatct tttgatgaaa ttctatgaga ttgataaggg aagtattcac
     4501 attgatggtg tggataccaa ggctatgacg cgttcagaag tgcatgatgc cttttcaatg
     4561 gtcttgcagg atacctggct ctttgaagga actattcgag acaatctcat ctataatcaa
     4621 atagggatta gtgatgaacg aatgatggaa gctagtaagg ctgtgggaat tcaccacttt
     4681 attatgacct tgccagatgg ctatgatacc atcttggatg acaccgtgac cttgtctgta
     4741 ggacaaaaac aactattgac tattgctcgt gcccttctta aggatgcacc gcttttgatt
     4801 ttggatgagg cgacttcttc tgttgacaca cggacagagg aattgatcca aaaagccatg
     4861 gaccgtttga tggaaggacg cacatccttt gtcattgccc accgcttgtc aaccatccga
     4921 aatgcagact tgatcttggt catgaaagat ggaaatatca tcgagcaagg caactatgag
     4981 gaactgatgg cgcaaggtag cttctacgct gacttgtaca atagtcaatt tacagaagat
     5041 gaagcagaag aataaatcaa aagaaggctt gacggccttc tttttcttat ttatactagt
     5101 acacatagaa agagagaagt atgagtcaga aattatacaa tatgaaattt gctgctgtct
     5161 acttagcctt gattgcaaaa gttgagcgaa agggtggaaa ggcagagtca gttcaccaag
     5221 tgaccagttg gttgacaggg tatgaagtga gtgacgttct tacttgtctg gatagggatg
     5281 ttacttacgg agattttttt
//
LOCUS       DAVBCG010000123         4988 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBCG010000123 DAVBCG010000000
VERSION     DAVBCG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.123"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(44..283)
                     /locus_tag="VQ495_001763"
     CDS             complement(44..283)
                     /locus_tag="VQ495_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2531688.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(303..575)
                     /gene="rpsP"
                     /locus_tag="VQ495_001764"
     CDS             complement(303..575)
                     /gene="rpsP"
                     /locus_tag="VQ495_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2531689.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            745..954
                     /locus_tag="VQ495_001765"
     CDS             745..954
                     /locus_tag="VQ495_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531690.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            1433..1654
                     /locus_tag="VQ495_001766"
     CDS             1433..1654
                     /locus_tag="VQ495_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531691.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            1737..2540
                     /locus_tag="VQ495_001767"
     CDS             1737..2540
                     /locus_tag="VQ495_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2531692.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(2596..4137)
                     /locus_tag="VQ495_001768"
     CDS             complement(2596..4137)
                     /locus_tag="VQ495_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2531693.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(4139..4621)
                     /locus_tag="VQ495_001769"
     CDS             complement(4139..4621)
                     /locus_tag="VQ495_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2531694.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(4621..>4988)
                     /locus_tag="VQ495_001770"
     CDS             complement(4621..>4988)
                     /locus_tag="VQ495_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET2531695.1"
                     /translation="KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNI
                     KKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQ
                     LLFNTMKRDRQKAVAAVNP"
BASE COUNT         1529 a         1058 c          935 g         1466 t
ORIGIN      
        1 cttctattcc acacaaaaaa gcgagacatc cgtcccgccc ttcttatttt tcgtcaataa
       61 cgattcttac ttttttgtat tcagttggga cagagtagac aatcgttctt atcgcagaaa
      121 tagtgcgacc cttacgaccg attacacgac ccacatcgct ttgatcaaga ttcaaatgat
      181 attccaaaaa ttctggtgta tcctcaatct tgatagttaa ggcatctggt tgtgaaatta
      241 agggtttcac aatcgcaata atgagatttt caatcgtatc catctgtcaa cctactttaa
      301 acttattttg aaaatttaga atcgtggaat tttttcaata cgccttcttt tgaaaggatg
      361 ttacgtactg tgtctgaagg ttgagctcca ttagccaacc atgcaagaac gcggtcttct
      421 ttcaaagtta cttggttttc agcaacaagt gggttgtaag ttccaactgt ttcgatgaaa
      481 cgtccgtcac gtggtgaacg tgaatctgct acgttgatac ggtagaaagg ttttttctta
      541 gaacccatac gagtcaaacg gattttaact gccattttta aagtctcatt tctttaattt
      601 tttatttcgg tgaaatagct gagctattta gcacatgttc tattatagca gatttctggc
      661 atgtgtcaag aaaaaaactt gacagactat aaatttctta aagtatttag aaatttgtta
      721 gaataggaaa taaatgtttg aaagatgcta tccgtgaata ctattttaga aaaattttat
      781 aaagaacacc aagtcaaacc cttcatatcg cctgaacgag agttggatac ttggcttcta
      841 agtcccaagc ccgttcccaa gcgaaatatg gacttattag tagatgattc actagcagga
      901 gatatcattt tactatggag aatccagttt ggtactttta ccactgaaac atgattttaa
      961 aaactcccat tcgaatatga ttcaaaattc gcttagaatc aatgttttcc cgctttttca
     1021 aaaaataaat tttcatctaa tttaaataac tttcaattaa gtgtgaattt tgcttaaaat
     1081 cattaaaaag caccccatgg tgtgaaccat gaagtgttgt aaatgctgtg ttgtagctag
     1141 ttcttagcgt ttagagaact ggctagcttt acgagctttc ttaagatctg gtttccaaaa
     1201 tactttgagt acacttatgg tattgatgcc cctaaacatc taaaaactct ggtagaaaaa
     1261 gggtatgctc ttgtcgaaac ttcttttgac tcacttgacc atctaaataa taccatgaaa
     1321 aagaatatcc tgaagaagaa gggagttact ggactttcta aaatgaaatc ttgcgacctg
     1381 gatcaagccc ttcatgagca tttttcagaa gaagaattag ctggtcactt tcatgtcctt
     1441 ctatggactt ttttacaatg gcattgctat cacacccaat acctatctaa gcgcctggtt
     1501 cgtaaacttt attgcagctc ttcctctaaa tttcctaatt gttgaaccaa ttgcccgttt
     1561 tatactaagt tcttttcaga aaccatttac tggggaagaa gttgaagaag ttgaagattt
     1621 tcaagatgat gatgaaatcc caactattat ctaagccagt tctgtaaact actaatattt
     1681 gaaatccact tctttttagg gtacaatggt ataaatgaat ttttgagagg atcagaatga
     1741 aaaaactagc aacccttctt ttactgtcta ctgtagccct agctgggtgt agcagcgtcc
     1801 aacgcagtct gcgtggtgat gattatgttg attccagtct tgctgctgaa gaaagttcca
     1861 aagtagctgc ccaatctgcc aaggagttaa acgatgcttt aacaaacgaa aacgccaatt
     1921 tcccacaact atctaaggaa gttgctgaag atgaagccga agtgattctc cacacaagcc
     1981 aaggtgatat tcgcattaaa ctcttcccta aactcgctcc tctagcggtt gaaaatttcc
     2041 tcactcacgc caaagaaggc tactataacg gtattacctt ccaccgtgtc atcgatggct
     2101 ttatggtcca aactggagat ccaaaagggg acggtacagg tggtcagtcc atctggcatg
     2161 acaaggataa gactaaagac aaaggaactg gtttcaagaa cgagattact ccttatttgt
     2221 ataacatccg tggtgctctt gctatggcta atactggtca accaaacacc aatggcagcc
     2281 agttcttcat caaccaaagc tctacagata cctcttctaa actccctaca agcaagtatc
     2341 cacagaaaat tatcgaagcc tacaaagaag gtggaaaccc tagtctagat ggcaaacacc
     2401 cagtctttgg tcaagtgatt gacggtatgg atgttgtgga taagattgct aaggccgaaa
     2461 aagatgaaaa agacaagcca actactgcta tcacaatcga cagcatcgaa gtggtgaaag
     2521 actacgattt taaatcttaa aaaccaaaaa aatacagtat ccacattcgg tactgtattt
     2581 cttttactct cattcttaag ttaaattatt aaaatcccat atttggtcta tccagccttc
     2641 ataaaagtct ggctcgtggc agaccataag gatagatccc ctatattctt tgagagcgcg
     2701 tttgagctca tcctttgcat ccacatccaa atggttggtc ggctcgtcca gcactaaaac
     2761 gttgttttca cgattcatca agagacagaa acgaaccttg gcttgctctc cccctgataa
     2821 tacttgaatc tggctttcaa tatgtttggt tgtcaaacca caacgggcaa gggctgcacg
     2881 gacttctgct tgattaaggg caggaaaggc attccagaca gcttcaagag gagtttggcg
     2941 attaccgcct tctacttcct gctcaaaata accaagttct aaataatctc cacgctccac
     3001 ttccccagcg attggcgaga taatgcccaa gagactcttc aagagagttg tttttccaat
     3061 accattagca ccaataatcg caaccttttg attgcgttcg aaggtaagat ttaaaggctt
     3121 agtaagagga aggtcgtaac caatttgcaa gttcttggct tggaagataa agcgccctgg
     3181 tgtacgagct ggtttgaaat caaaggatgg ttttggtttc tcactttgga gttcgataat
     3241 atccatctta tccaatttct tttgacgaga catagccata ttacgagttg caacacgggc
     3301 tttattacga gccacaaagt ccttgaggtc tgcaatctct ttctgctggc gttcgtaggc
     3361 tgcctctagc tgagatttct tcatagcata aacttcttgg aactggtagt agtcaccaga
     3421 gtaacgcgtc agctgttgat tttccacatg atagacaata ttgataacgt cattgaggaa
     3481 tggaatatcg tgcgaaatga gaacaaaggc attctcatag ttttggagat agcgcttgag
     3541 ccaatcaata tgctcagcat ccaagtagtt ggtcggctcg tccaacagca agatatcagg
     3601 cttttcaagg agaagttttg ccaaaagcac cttggttctt tgcccacctg acaaagaagt
     3661 tacatccgta tccatgccaa agtccataac accaagagca cgcgctactt cgtcaatctt
     3721 agcatccaag gtatagaaat cacgactctc cagacggtct tgaagttctc ctacttcttc
     3781 catgagagca tcaacatccg caccgtcttc agccatttcc atatagaggt cattgatacg
     3841 agcttcagct ttgaaaagct catcaaaagc cgtacggaga acatcacgca ccgactgtct
     3901 ttcagcaagg acagagtgct gatccaagta accagccgtc acatatttgg accactcaac
     3961 ctttccttca tctggcagca ttttaccagt cacgatactc ataaaggttg attttccttc
     4021 accattggca ccgaccaggc cgatatgttc tcccttgagg agacggaagg acacatcttc
     4081 aaaaattgca cggtcaccaa aaccgtgact cagattttta acttctaaaa tactcatttt
     4141 aattccttat cttgttttta tgtaatcgtt tataaaggag ccaagccaga tagccaccca
     4201 aagtgttggt ccacaaatca tcaatctcaa agacgcgatt gaaatcaaag aaaaagtcca
     4261 agattaattg cgtacactcg attccaagac tcacaagaaa actaaaaaga aggacctttt
     4321 ttgttttccg caaatttgga aatagataaa ggagttggaa aatcagagga aaaaacaaga
     4381 agacattgag gatattttgt aaaaaaatcc aacataattg tccaatgtca ctcacttcgc
     4441 ccagtttcca gagagaattg aaaggagtca aaagaaaaac caggcgtcca agatgctgaa
     4501 tatctggagt tcccactccc acggtagatt gttcttgagg agtaaagcaa aaatagacaa
     4561 tgcaaatgct atagaaaatg actccccaga ccaaaatatg attataagtc ttcttcatca
     4621 ttaaggattt accgctgcga ctgccttctg gcggtcacgt ttcattgtgt taaagagcaa
     4681 ttgtccacaa gctgcgtcaa tatctgtacc atgctcttga cgaaccacac agttgccccc
     4741 ttttttctta agcgtatcat agaaagccaa cacgcgctct ttgggactac ggctatattg
     4801 gtcatgctca ctaactgggt tataaggaat caagtttaca taagacgatt tcttgatgtt
     4861 cttgagcaat tcagccaatt ccaaggcttg ttccacaccg tcgttgactt cattaagcat
     4921 gatatattca aaggttacac gacggtttgt tgtctcaatg tagtattcaa tagcagcaaa
     4981 gagttttt
//
LOCUS       DAVBCG010000124         4862 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBCG010000124 DAVBCG010000000
VERSION     DAVBCG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.124"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            98..1357
                     /locus_tag="VQ495_001771"
     CDS             98..1357
                     /locus_tag="VQ495_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2531696.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..2107
                     /locus_tag="VQ495_001772"
     CDS             1607..2107
                     /locus_tag="VQ495_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2531697.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2183..2551
                     /gene="rplL"
                     /locus_tag="VQ495_001773"
     CDS             2183..2551
                     /gene="rplL"
                     /locus_tag="VQ495_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2531698.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            2738..2908
                     /locus_tag="VQ495_001774"
     CDS             2738..2908
                     /locus_tag="VQ495_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531699.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2908..3156
                     /locus_tag="VQ495_001775"
                     /pseudo
     CDS             2908..3156
                     /locus_tag="VQ495_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3170..3510
                     /locus_tag="VQ495_001776"
                     /pseudo
     CDS             3170..3510
                     /locus_tag="VQ495_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3503..3883
                     /locus_tag="VQ495_001777"
     CDS             3503..3883
                     /locus_tag="VQ495_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2531700.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            3896..4039
                     /locus_tag="VQ495_001778"
     CDS             3896..4039
                     /locus_tag="VQ495_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531701.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            4132..4719
                     /locus_tag="VQ495_001779"
     CDS             4132..4719
                     /locus_tag="VQ495_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2531702.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
BASE COUNT         1535 a          828 c         1034 g         1465 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg
      481 tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagatc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgcga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca aagaagacgc
     2101 ggcttaatct taagctacac agcgtagcct agctacgaaa aaaactatta taaaatttaa
     2161 aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg ctgaaattaa
     2221 agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag aatttggtgt
     2281 aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg gtgctgctaa
     2341 agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg ttatcaaagt
     2401 tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg acggtgcacc
     2461 agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaaa ctaaattgga
     2521 agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat tgcggaatcc
     2581 ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat tgatttggtt
     2641 tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg gtagacacta
     2701 gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag gacagtcatc
     2761 acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg taaaagcaac
     2821 tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat ttggaaatgt
     2881 tggaatttta gtcttggagg gacaataatg aagtgtatta ccgctaatca atatcagaca
     2941 tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta taagaatatg
     3001 aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt gtctttgagc
     3061 aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc aaatttgatg
     3121 gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag tgacattttt
     3181 agatgattac cataaaaaac ataactaccc actattttac gaatcctatc ttcaaaacgt
     3241 tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg tagatgatca
     3301 tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa aagagggaat
     3361 acttacaccc caagtaactg taaaagctta tgatgaagac aagaaaccga ttaacttcgc
     3421 aaatttatta gattccttaa tttattagat tccttaatcg tgtcagaata tcaaatggaa
     3481 ccgaatcttt gggaggtctc ccatgattga tctctatcta agtaaaaata gacaaagaaa
     3541 tcaacttctt ttagatttct tccaaaacta tggcatagag gtatcttgtc attcagtttc
     3601 tgaaatgaca aaggacaaat taattgagat gatgagctat tcttcagatt gttttgagtt
     3661 tttatctccc aatttattac gatttaagaa ccgtgacaat ctaagattaa cggatttcgt
     3721 tgagataatt ttaaaaaatc ctgaactaac catcagactt cctcttgcgg tttcaaataa
     3781 gcgagtttat ccaagtctga atttggaaga ggctagagct ctattgccaa gagatacgaa
     3841 acaattgatt tacatggcgc aaacacatta tttatctaac taaaggagaa gcaatatggc
     3901 tgaacgattt tgggagaact tgtccattat tttggctgaa agaaatatca gctggattga
     3961 gttaaccaga aaaatgtttg cgggagagtt tcactatcca agtgaattga atcgtttgta
     4021 tcaaaagatt cgccactaga agatggaaca atgaatgcct caaagtccat gggtagaaag
     4081 gattgtgcag gtattagatt tagattatga agatttattt cggaggtaac tatgaaaaga
     4141 ataattccag tttatatatt ccaacaagta aatgtcctat tggtatctct atacttattg
     4201 aaatttcttt gtatcggtga gttaactata ctacagattc tctatggttc gtcactcatt
     4261 tcttttttat ggatgtatgg ccaacgcaaa caagcgcata aggtcaatat gaaatctagg
     4321 atgaaatggc ttggtgttga atttgttagc ctactgatta taagtctatg ttttagtctg
     4381 attcatgctc aaggaagcac gaatcaagca aacttaattg gccttcaaca tcaaattcct
     4441 tggttttcat ttttattgtt cttaatcaat gcgagtatgg ttgaagaatt tctgtatcga
     4501 gaaattttat ggaatttggt cagaaaatta gatatcagag ttgctttgac aagtgtttta
     4561 tttgccttag cacatcatac aggaaccatt ctagcttggt gtttgtatgt ttcacttggg
     4621 atgtttttag gtatggtgcg ctataaatct gacttatggg gcagtatggg actacatttg
     4681 gtgtggaacc tactagttta tagtttgctg cttttttaaa ataggtgacc tgatttttag
     4741 gtaacttcta aaactaactt ccagttttgg gagagagatg gaagttactt tgaaaagtta
     4801 cggaaacatt attttctatg tgacaacatt gtataattgc ttgcatttta ctggtggaga
     4861 ga
//
LOCUS       DAVBCG010000125         4845 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBCG010000125 DAVBCG010000000
VERSION     DAVBCG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.125"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            165..383
                     /locus_tag="VQ495_001780"
     CDS             165..383
                     /locus_tag="VQ495_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2531703.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            1428..2420
                     /locus_tag="VQ495_001781"
     CDS             1428..2420
                     /locus_tag="VQ495_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2531704.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2422..3240
                     /locus_tag="VQ495_001782"
     CDS             2422..3240
                     /locus_tag="VQ495_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2531705.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3242..4027
                     /locus_tag="VQ495_001783"
     CDS             3242..4027
                     /locus_tag="VQ495_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531706.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4066..4344
                     /locus_tag="VQ495_001784"
     CDS             4066..4344
                     /locus_tag="VQ495_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2531707.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4329..4526
                     /locus_tag="VQ495_001785"
     CDS             4329..4526
                     /locus_tag="VQ495_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531708.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1397 a          791 c         1015 g         1642 t
ORIGIN      
        1 ttttcattta gtataacaga ttgttagaag ttggtttaaa tttcctaatc agtttgttca
       61 catttacctt cgatatatta tatcccatag ttaaggttgg tcatacagat gattatagtc
      121 atggagccgt aaaacttagt gtttctttag ttgacaaaga tgccatgaaa aaaatatctg
      181 taactgtaat aggatatttt gaaataaata tagatgaaaa tatcaccgat attctatacg
      241 taaatggtac tgctattctt tatcattatt tacgttctat tgtttcaata gtttcggcaa
      301 ttgatagcag tgaagcaatg ttgctaccta ccattaatgt tttagagtta ctagataaat
      361 ctcaaccttt tgaagaagaa taatttatta gctcactaaa ttgagggtaa ggaaaagtaa
      421 aatcagtaag aaaaatgtct tgcattatac agcaaccttt tgggaatgag tagatggatt
      481 gaataaaatt ttattaagag tggatgattt atctgtagat tattattgga cagttagtct
      541 tgaagtagtc taagaattag gttataatca gtagaatcct tgctaataat gaggaggtta
      601 gtttatgtat agtagactga atctaaaata gtacgaaaca attgctaaaa catttataga
      661 aattaatttt actttcccaa tcgatttgtt ctcatcttat ttcaatccgc tatatattat
      721 ggtatcgaat cttcatcaga atgataaaat taatcaattg atatctgatt acaaacagaa
      781 tatgaaagct ttttatatca ctattgaaaa atttatacga gatgatgaaa gccttaagtg
      841 ttattttata aaggttattt caagtcgttc caaggtaaca agtctagatc agattgaagc
      901 tgataaaacg atacaaagaa aatattcaag tgagctaaaa aaatttattg gattttataa
      961 tgagattatt tgtgaggaaa atagtttcct acatgtacga aagaggtggt cgagttggtt
     1021 taggtagtcg atgcgtgagt tgataattct cagggtatgg acttcttttt catgaatgag
     1081 gtaaaagagc aggtattgtt tagagacaat cattctgagc atattttctg gatagaggga
     1141 gtatccgatt ttatgatcaa agttaatacc gccctctggt gagaagatga gtaggttggt
     1201 aatttaaact attaaacaga atttttgatt aaaagtatta ttttatgaga gaaatcctaa
     1261 tttcacaatc cataggcaaa ctcttgcatt tcgtttttta ttggactata ataggttggt
     1321 ataaagcctt ttgtagtaat aaaatgtaga aggtgtagaa agcaaggatt tagaatattt
     1381 gtagttaaaa acacaatgtt gctattcctt acgataggga gatagatatg gcaatgatag
     1441 aagtggaaca tcttcagaaa aattttgtga agactgttaa ggaaccgggc ttgaaggggg
     1501 ctttgcgctc ctttattcat cctgaaaagc agacctttga agcggtcaag gatttgacct
     1561 ttgaggttcc aaaagggcag attttaggat ttatcggggc aaatggtgct gggaagtcga
     1621 caaccattaa aatgctgaca ggaattttga aaccaacatc tggtttttgt cggattaacg
     1681 gcaagattcc ccaggacaat cggcaagatt atgtcaaaga tattggcgta gtctttggac
     1741 aacgcaccca gctatggtgg gatttggctc tgcaagagac ctacactgtc ttaaaagaga
     1801 tttatgatgt gccagactcg ctctttcata agcgtatgga ctttttgaat gaagtcttgg
     1861 atttgaagga ctttatcaag gatcccgtgc ggactctttc actgggacaa cggatgcggg
     1921 cggatattgc ggcctccttg ctccacaatc ccaaggttct ttttttagat gagccgacca
     1981 ttggtttgga cgtttcggtt aaggataata ttcgtcgggc aattactcag atcaatcaag
     2041 aggaagaaac taccattctt ttgaccactc acgatttgag tgatattgag caactttgtg
     2101 atcggatttt catgattgac aaggggcaag agatttttga tggaacggtg agccaactca
     2161 aggagacctt tggtaagatg aagactctct cttttgaact gctaccaggt caaagtcatc
     2221 tcgtctctca ctatgacggt ctgtctgata tgaccattga tagacaagga aacagcctca
     2281 acattgaatt tgatagttct cgctaccagt cagctgacat tatcaagcaa accctgtctg
     2341 attttgaaat ccgcgatttg aagatggtgg atacggatat tgaggatatt atccgtcgct
     2401 tctaccgaaa ggagctctag gatgatcaaa ttgtggagac gttataaacc ctttatcaat
     2461 gcaggggttc aggagttgat tacttaccga gtcaacttta ttctctatcg gattggcgat
     2521 gtcatggggg cttttgtggc cttttatctc tggaaggctg tctttgattc ttcgcaagag
     2581 tctttgattc agggcttcag tatggcggat atcaccctct acatcatcat gagttttgtg
     2641 accaatcttc tgactagatc cgattcgtcc tttatgattg gggaggaggt caaggatggc
     2701 tccattatca tgcgtttgtt gcgaccagtg cattttgcgg cctcctatct tttcaccgag
     2761 cttggttcca agtggttgat ttttatcagc gttggccttc catttttaag tgtcattgtc
     2821 ttgatgaaaa tcatatcggg tcaaggtatt gtagaggtgc taggattaac tgtcatttat
     2881 ctttttagct taacgctcgc ctatctgatt aactttttct ttaatatttg ctttggattt
     2941 tcagcctttg tgtttaaaaa tctttggggt tccaacctac ttaagacttc catagtggct
     3001 tttatgtcgg ggagtttgat tcccttggca ttctttccaa aggttgtttc agatattctc
     3061 tcctttttgc ctttttcatc cttgatttat actccagtta tgatcattgt tggaaaatac
     3121 gatgccagtc agattcttca ggcactcctt ttgcagttct tctggctctt agtgatggtg
     3181 ggattgtctc agttgatttg gaaacgggtc cagtccttta tcaccattca aggaggttag
     3241 tatgaaaaaa tatcaacgaa tgcatctgat ttttatcaga caatacatca aacaaatcat
     3301 ggaatataag gtggattttg tggttggtgt cttgggagtc tttctggctc aaggcttgaa
     3361 tctcttgttt ctcaatgtca tctttcaaca tattccattc ctagaaggct ggacctttca
     3421 agagatagct ttcatttatg gattttcctt gattcccaag ggaatggacc atctcttttt
     3481 tgacaatctc tgggcactag ggcaacgcct agtccgaaaa ggggagtttg acaagtatct
     3541 gactcgtccc atcaatcctc tctttcacat cctagttgaa acctttcaga ttgatgcctt
     3601 gggtgaactc ttagtcggtg gtattttatt gggaacaaca gtgaccagca ttgtttggac
     3661 tcttccgaaa ttcctgcttt tcctagtttg tattcctttt gcgaccttga tttatacttc
     3721 tcttaaaatc gcaacagcca gtatcgcctt ttggactaag cagtcaggcg ccatgattta
     3781 catcttctat atgttcaatg actttgctaa gtatccgatt tctatttaca attctcttct
     3841 tcgttggttg attagcttta tcgtgccttt cgcctttaca gcctactatc cagctagcta
     3901 tttcttacag gaaaaggatg tgttctttaa cgtaggaggt ttgatgttga tttctctggt
     3961 tttctttgtt atttccctta aactttggga taagggctta gattcctacg aaagtgcggg
     4021 ttcgtaaaag ctaaagtaag actaaaatca agaaagaaac ttatgatgtt tgtaattgaa
     4081 gaagtcaagg atgaaaatca aaaaaaggca gttgtagctg aggttttgaa ggatttgcca
     4141 gaatggtttg gaatcccaga aagcacacaa gcctatatag aaggaaccac gacactgcaa
     4201 gtttggaccg cctatcagga gagtgatttg actagatttg taagcttatc ctattcgagt
     4261 gaagattgtg cagagattga ttgtctcggc gtaaaaaagc ttatcaaggt agaaaaattg
     4321 ggagccaatt gcttgctact ttagagagtg aagctcgtaa aaaagttggt tatctgcagg
     4381 tcaaaacagt ggcagaaggt tctaataaag attatgatcg aacaaatgac ttttatcgag
     4441 gtcttggctt taaaaagtta gagatttttc ctcaactatg gaatccgcaa aatccttgtc
     4501 agattttgat taaaaagctt gaataatatt acttgacatc tattctcaga gtgccatact
     4561 gtaagtgtaa tcgccgattt agctcagttg gtagagcaag gcactcgtaa agcctaggtt
     4621 ataggtagat aaacgactga ggagttgaaa aaatagatag gtagaagata accgttaagc
     4681 cttattctta gcggttattt atattgttta atagcgctga tatttcatca attatgcctg
     4741 ttttcgtgtt tctggtagtt gttcaagttt attgctacta tttttgatgg tatgaatgtg
     4801 cttataatgt atcccggtta acgaaagttt tggacttata ctctt
//
LOCUS       DAVBCG010000126         4767 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBCG010000126 DAVBCG010000000
VERSION     DAVBCG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.126"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(6..1220)
                     /locus_tag="VQ495_001786"
     CDS             complement(6..1220)
                     /locus_tag="VQ495_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2531709.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1376..1669)
                     /locus_tag="VQ495_001787"
     CDS             complement(1376..1669)
                     /locus_tag="VQ495_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531710.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1831..2283
                     /locus_tag="VQ495_001788"
     CDS             1831..2283
                     /locus_tag="VQ495_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2531711.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2318..3706)
                     /locus_tag="VQ495_001789"
     CDS             complement(2318..3706)
                     /locus_tag="VQ495_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2531712.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3772..4734
                     /locus_tag="VQ495_001790"
     CDS             3772..4734
                     /locus_tag="VQ495_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2531713.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
BASE COUNT         1395 a         1112 c          945 g         1315 t
ORIGIN      
        1 aagccctatc tacgatactg tttcaagaaa cgagtcatct tttcttggat ttgggctaac
       61 tcatcaacac gaggaaggta aacgatacgg aagtggtctg gttcctgcca gttaaagcct
      121 cgaccatgaa ccaagagaac cttttcctgc ttcaagaaat caaggacaaa ctgctcatca
      181 tcatcgatac ggtacatatt gcggtcgatt tttgggaaga tatagagccc cgccttgggt
      241 ttaacggcag acaaacctgg aatatcttga atggcattat agatgaaatt tctttgctcg
      301 tagattcgtc caccaggaag aagcaattca tcgactgatt ggtgaccccc caaggaagtt
      361 tgtacgactt gttgggccaa aacgttagag caaaggcgca tattggacag catattgagc
      421 ccttcgatat agcccttaac atgagtctta gggccagaca agaccatcca acccacacgg
      481 aaacctgcta tgcggtggga ttttgacaga ccattcatgc tgacacagaa gacatctggt
      541 gccaagctcg ccacaggcgt atgcacatgt ccgtccatta ccatgcggtc atagatttca
      601 tccgcaaaga taatcaaatc gttttgacgg gcaatctcaa taatctccag caagagttcc
      661 ttaggataaa gggctccagt tgggttattt ggattgataa ggacaattgc cttggtattg
      721 gaagtgattt ttgacttgat atcgtcaata tctgggtacc attccacagc ttcatcacag
      781 atatagtgaa cggcatttcc tccagctagg ctgacagccg ccgtccatag aggatagtct
      841 ggcattggca ctaagacctc atccccattg tccaaaagcc cctgcatgga catgacaatc
      901 aactcactga caccatttcc aaggtaaata tcatcaatat ctacgtttgg gaatttcttc
      961 agctggcaat actgcatgat ggccttacgg gctgagaaaa tccctttgga gtcagaatag
     1021 ccttcactat cacgcgcatt cataatcaag tcatgaatga cctcgtctgg cgctgtaaag
     1081 ccaaattctg ctggatttcc tgtattcaga cgtaaaatct tttctccatt cgctcgcatc
     1141 cgcatggctt cttccaatac aggaccacgg atatcataag caacatgctc taacttacta
     1201 gacttgttat attctttcat cttgtttcct caattcatca ggatagtaac attataccac
     1261 tagaaagaga atgcgacaag tttactgaaa tgtgttacta gtttcgtata gaagaaaaac
     1321 cttgctaaag gcgaggttaa gaacgtagag atagcaggca ttttgcaaag acacatcaca
     1381 taccaaaaga taaacctaga aaaagcaagc ctagaaagga gtcataaaga agattaaaca
     1441 tgaggaaaag cccccatatc atcaagtaat cccaacgccc acttcgtttt gcaactagaa
     1501 ataagattag atggtagagt acatgaaaga ctaaaaatcc aataaaactt ggataaagaa
     1561 gcggtaccag actctctaat tgacagatca tgatcacttt taccaagact gaaactagga
     1621 taattccata atacaggaaa tttgattttt gaaaagagtt tttcatcatt ctcccttcct
     1681 tcacttcttt ccttctatcc tttcttttat tttatcatat atttatatag attttaagcc
     1741 ctaatattct tttgaaaaat actttttttg ctttacttct atagtgaaaa ggtttacaat
     1801 tatagtaaga aaatttcagg aggtttcatt atggcacaac gttaccaaaa tatcatggtc
     1861 gcaatcgatg gttctaagga agcggacttg gcttttgtca agggagttca ttctgctcta
     1921 cgaaacgacg ctaaactcac catcgcacat gtcattgaca cacgcgctct ccaaagcgta
     1981 tccacctttg atgctgaagt ttacgaagaa ctccaagtcg acgctgaaag tctgatgaaa
     2041 gagtacgaaa aacgtgctaa agatgctggg gtagcagatg ttcatatcgt cattgaaatg
     2101 ggaaatccaa agaccctgct agcacgtact attccagatg ccgaggaagt ggacctcatc
     2161 ctcgttggcg caactggtct caacgccttt gaacgcctct tggtcggctc ttcatctgaa
     2221 tacatactcc gccatgctaa ggtcgatttg ctggttgtga gagaacaaga aaaaacctta
     2281 taatcacaaa gaaaaggagc ccctagctcc tttttgttta cgatttattt ctctctttat
     2341 ggcgttcgta agccttgagc tggcgctgca gttccttttt aatagcaggt tctggagcat
     2401 atttttcttc ccaattatct ggttttaaga ttttatgggt cactggatca aaatgagcct
     2461 tgccatctgg aaaaattttc cccatattgg cctgatggac aatatcaaaa atacgttctg
     2521 ggtccacccc catcaagaca aaactgccgt aggtgaagta aagcgtgtca atcaaggcat
     2581 ccacttgccc tatcaaatct tgctgagcag gtgtcttctt ggctacttta cctgctgcct
     2641 tatcaagggc ctgatgaagt tgcgatacag cttgaccaaa atcttcttca gaaggactgg
     2701 ctgctcgaac aaactccacc aattcttcta ttttaaaacc agccctatgg gttgcaccct
     2761 ctaaatccca agctcgaggt tcttcttggg ttcgttcatc catcatgtgg tggaaggtct
     2821 tgaccttatt gaaatgatag tcacggctga caaagacttt ttctgaagac aaaacaccaa
     2881 aatgttccaa ggccttgtgg atcccatctt gttgattgct agtggtaatg tgcttggcag
     2941 cttccttgac actgctacta ccatttccca tagcgaccga cataccgacg ccagccagca
     3001 tttcaaggtc attatctgag tcaccaaaag ccatgacttg gttgaggtca aagccatatt
     3061 ctttcccaac tcggcgaatg ccttctaatt tagaatttcc ctgattgatg acatccgatg
     3121 caaaaggatt gctacgtgtc aatttcaagt cttcaaaatc agctgccgcc ttctcagatt
     3181 cttctggtgt catcagcatc aaaacttggt agataggctg attcatcagg tgaagcaggt
     3241 cctcttcctt ttggggaaca accttgctga ccatgcgatt aaaagactga ctcaccgtcc
     3301 gagttaaaac agagggaacg aagcgactaa ttcgttggga aaaagaaccc agaccaaagg
     3361 acatgatttt agaacccaac atggcatcct tggtccctag ggcaatctcc gtgccctctt
     3421 ttttagcata gctaattaga tggcgcaaat gtaacttgga aatagggctc gtgaacaaga
     3481 ctctgtcttt actaaagata tactggccat tataggttac cgcaaaatcc agatccaaat
     3541 cgtccatcaa ttccttaaca aaaaaaggtc ctcgccctgt cgctacgcca actagtaccc
     3601 cttgttcttt gacaatctta atcgcgtcct tagtggattt caaaacactc ttgcgattgt
     3661 tgaccaaggt tccatcgata tcaaaaaaaa cagctttgac ttccatccta ttccaatctc
     3721 cccttttgtg atacaatgat tataccacat ttcagaaaga gtgagtaaat catgcctaag
     3781 aaaatccttg ttttacatac gggtggaact atttccatgc aggccgatgc ttctggcgct
     3841 gttgtgacga gttcagataa tcccatgaac catgtgtcca acccacttga aggaatccaa
     3901 gtccacgcct tggacttttt taaccttcca agtccccata tcaaacccaa acatatgctg
     3961 gtcctctacc agaaaattaa agaggaagca gataactacg atggagtggt aatcacacac
     4021 ggaaccgata ctttagagga aacagcctat ttccttgata ccatggaagt tccccatatg
     4081 cctatcgttc taacaggagc catgcgtagc tccaatgagc tcggtagtga tggtgtttat
     4141 aattacctaa gtgctttacg agtggccagc gatgacaggg ctgctgacaa aggagttttg
     4201 gtcgttatga acgatgaaat ccacgctgcc aagtatgtca ccaaaacaca tacgactaat
     4261 gtcagcacct tccagactcc aacacatggc cccctcggtc tcatcatgaa acaggaaatc
     4321 ctctacttca aaacagctga acctcgtgtt cgctttgacc ttgatcacat acaaggttta
     4381 gtccctatca tctcggctta tgctggtatg acagatgagc tgattgatat gctggattta
     4441 gaacacttgg acggtttgat tatccaagcc ttcggagctg gtaatattcc caaagaaacg
     4501 gctcaaaaat tagaaagcct tctgcaaaaa ggaatcccag tcgctctggt atcacgatgc
     4561 tttaacggta ttgccgagcc tgtttatgcc taccagggtg ggggcgtaca gttgcaaaaa
     4621 gcaggcgttt tctttgttaa agaactcaac gcccaaaaag ctcgcttgaa actcctcatc
     4681 gccctcaatg ccggactaac aggacaggct ttgaaagact atatggaagg ctaatactct
     4741 tcgaaaatct ctgcaaacca cgtcaga
//
LOCUS       DAVBCG010000127         4746 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBCG010000127 DAVBCG010000000
VERSION     DAVBCG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.127"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..929
                     /gene="pncB"
                     /locus_tag="VQ495_001791"
     CDS             <1..929
                     /gene="pncB"
                     /locus_tag="VQ495_001791"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2531714.1"
                     /translation="ANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYAATHK
                     NCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLDEAGF
                     TEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIEDETG
                     QMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKMFHPT
                     YTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRVLNPQ
                     HYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            926..1750
                     /gene="nadE"
                     /locus_tag="VQ495_001792"
     CDS             926..1750
                     /gene="nadE"
                     /locus_tag="VQ495_001792"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2531715.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            2148..2699
                     /locus_tag="VQ495_001793"
     CDS             2148..2699
                     /locus_tag="VQ495_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531716.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            3399..4106
                     /locus_tag="VQ495_001794"
     CDS             3399..4106
                     /locus_tag="VQ495_001794"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2531717.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            4160..4549
                     /locus_tag="VQ495_001795"
     CDS             4160..4549
                     /locus_tag="VQ495_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2531718.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT         1547 a          830 c         1060 g         1309 t
ORIGIN      
        1 gcgccaatgg aaccagcaac gtgcgtgcgg gtaagctctt tgacattcct gttttgggaa
       61 cccatgccca tgccttggta caggtttatg gcaatgacta tgaagctttc aaggcttacg
      121 ctgcgaccca caaaaattgt gtctttcttg tggataccta tgacaccctt cgcatcggtg
      181 taccagctgc cattcaggtg gcgcgtgagc tgggtgatca gattaacttt atgggtgtgc
      241 ggattgactc tggggatatt gcctacattt ctaagaaagt ccgtcagcaa ctggatgagg
      301 ctggatttac agaggctaag atttatgctt ctaatgatct agatgaaaat accatcctca
      361 atctcaagat gcaaaaggcc aagattgatg tctggggcgt gggtaccaag ctgattacag
      421 cttatgacca gccagctctt ggtgcagttt acaagattgt tgcaatcgaa gatgaaactg
      481 gtcagatgcg caatacgatt aagctgtcta ataatgcgga aaaagtctct acgccaggta
      541 agaagcaggt gtggcgcatt accagtcgtg aaaaaggcaa gtcagaaggc gactatatca
      601 cttatgatgg tgtggatatt agcgacatga cagaaatcaa gatgttccat ccgacctata
      661 catacatcaa gaagacggtt cgtaattttg atgccgttcc tctcttggtg gatatcttca
      721 aagaaggaat attagtttac aacttgccta gtttgactga cattcaggat tatgcccgta
      781 aggaatttga caagttgtgg gatgagtata agcgtgtgct caatccgcag cactatccag
      841 tggatttggc gcgtgatgta tggcaagata agatggactt gattgataag atgcgcaagg
      901 aagcccttgg tgaaggagaa gaagaatgag tttgcaagaa acgattatcc aagagctggg
      961 tgtcaaacca gtgattgatg cccaggaaga aatccgtcgt tctattgatt tcttaaaaag
     1021 atatctgaaa aaacatccct tcctaaaaac ctttgtacta gggatttctg ggggacaaga
     1081 ctcaaccttg gcagggcgtt tggcgcaatt agctatggaa gaactgcgag ctgaaacggg
     1141 agacgacagc tacaaattta tcgctgtccg cctgccatac ggagtgcaag ctgatgaagc
     1201 agatgctcaa aaagccctag ccttcatcca gccagatgtc agcttggttg tgaatatcaa
     1261 ggaatcagct gatgccatga cagctgcagt tgaagcgaca ggtagtcctg tttcagactt
     1321 caacaagggg aatatcaagg cacgttgccg tatgattgct cagtatgccc ttgctggttc
     1381 ccatagcgga gcggtcattg gaacagacca cgccgcggaa aatatcacag gtttctttac
     1441 caagtttggt gacggcggtg cggatattct ccctctttac cgcctcaata aacgccaagg
     1501 aaaacagctc ttgcagaaac ttggcgcaga gccagccctt tatgaaaaaa tcccaacggc
     1561 agacctagaa gaagataaac caggcctagc tgacgaagtc gcacttggag tcacctacgc
     1621 agagattgac gactacctag aaggcaaaac aatcagccca gaagctcaag cgaccattga
     1681 aaactggtgg cacaaaggcc aacacaaacg ccacttaccc atcactgtat ttgatgactt
     1741 ttgggagtaa aaacctccat tggaggtttt taccctgagc ctagaaatga gaaagcgaag
     1801 aaggtccggg gaccttttta gcttcttgcc ctgaaattaa aaagcaagaa aaacctccac
     1861 tggaggtttt cagcctctca tcttgaaata agaaagtgag agaaggtctg ggggatcttg
     1921 aaccccgagt ttagaaataa gaaaatgagg cagattcggt aactcgaaga gttcgatttc
     1981 atcgtcttac ccctgcaacg atgactaggt ttgaaaaagc ttgctagagc gcatttcaaa
     2041 ccaggcagcg actgcgtcaa gaaattagaa gacaaactcg ttttctagct gttactgagt
     2101 tgagcctttt tactacgagc atagaaataa ggaagtgagg tagcatcatg aaatctatcg
     2161 gtacgcaaat attacagaca gaacgtttga ttttaagaag atttgtggag agtgatgcag
     2221 aagccatgtt tcaaaattgg gcttcatctg ctgagaatct gacctatgtt acctgggatc
     2281 cccatcctga tatcgaaatc actcgaaact cgattcgcaa ttgggttgct tcctatacta
     2341 atctcaacta ttataaatgg gccatttgtc taaaagaaaa cccagagcaa gtgataggag
     2401 atatcagcat cgttaagata gacgaggctg atttaagctg tgaaattggc tatgtgttag
     2461 gcaaggctta ctggggaaat ggtatgatga cagagacttt gaaagctatc ttggactttt
     2521 gttttactca agcaggtttt caaaaggtca gagcacgtta tgccagtctc aacccagctt
     2581 caggtcgtgt catggaaaag gctggaatgt cctatctaca aaccattgtt aatggtgtag
     2641 agagaaaagg ctatcttgcg gatcttattt attatggtat aagtagggaa gaatgttgaa
     2701 ttctattttc tgtttctatc gaagtcaact atttattgta aatataataa ttagcattcc
     2761 aagtttattt gaaactttaa aatagcatat tgattagtac aagacagatg ttctagttcc
     2821 ttctttaatc tggtttagta ttagttaaaa aatcgcttta agcttgtaac taagagggag
     2881 ctaatcgact agattctcca gccgaacagg tggtaatgta ctttttatag tgtaatccta
     2941 gctgttgtta aatttaaaat agaatcctct atcgagttag ggaattaaat tcaaccaatt
     3001 ttattcatgt tttttctatc aaattatcta atattaaaat agtctcattc tgatgagaaa
     3061 actattctat ttatactgca tagttgtata aggaatgatg aaagatgagt gaaaattatc
     3121 aagttggaat gtttgtatct aaatatatta gcatgtattt agataagatg tctgcaatcc
     3181 tttatatatg agattgatta cgatatagtt aaagtgttag ttcaataatc aatatatcat
     3241 tctaagtcta ttttatatat aaagtatatt gtttaacgtt tgatagaaaa gatgctatac
     3301 tagaagtagg atgtaatggc aaaatctaat actaataagc gctatgcatt acgtaaactc
     3361 ggaaagattt ttggttctgc ggtaatcggg ctaactttat ttttaggagg aatctgaata
     3421 caagttttgt tcatgctgct gatgggattc aatatgtcag agatgatact agagataaag
     3481 aagagggaat agagtatgat gacgctgaca atgggggtat tattgtaaaa gtagcgacta
     3541 aacctaaggt agtaaccaag aaaatttcaa gtacgcgaat tcgttatgaa aaagatgaaa
     3601 caaaagaccg tagtgaaaat cctgttacaa ttgatggaga ggatggctat gtaactacga
     3661 caaggaccta cgatgttaat ccagagactg gtcatgttac cgaacaggtt actgttgata
     3721 gaaaacaagc cacggataca gttatcaaag ttccagctaa aagcaaggtt gaagaagttc
     3781 ttgttccatg tgctactaaa tatgaagcag acaatgacct ttctgcagga caggagcaag
     3841 agattactct aggaaagaat gggaaaacag ttacaacgat aacttatgat gtagatggaa
     3901 agagtggaca agtaactgag agtactttaa gtcaaaaaga agactctcaa acaagagttg
     3961 ttaaaaaagg aaccaagccc caagttcttg tccaagaaat tccaatcgaa acagaatatc
     4021 tcgatgaccc aactcttgat aaaagtcaag aagtagaaga agtaggagaa attggtaaat
     4081 tactcttact acaatctata ctgtagatga acgtgatgga acaattgaag aaactacttc
     4141 tcgtcaaatt actaaagaga tggtaaaaag acgtataagg agagggacga gagaacctga
     4201 aaaagttgtt gttcctgagc aatcatctat tccttcgtat cctgtatctg ttacatctaa
     4261 ccaaggaaca gatgtagcag tagaaccagc taaagcagtt gctccaacaa cagactggaa
     4321 acaagaaaat ggtatgtggt atttttataa tactgatggt tccatggcaa caggttgggt
     4381 acaagttaat ggttcatggt actacctcaa cagcaacggt tctatgaaag tcaatcaatg
     4441 gttccaagtt ggtggtaaat ggtattatgt aaatgcatcg ggtgagttag cggtcaatac
     4501 aagtatagat ggctatagag tcaatgataa tggtgaatgg gtgcgttaac agattcctcc
     4561 ctgcgacaaa attgataata gtatgtctga atatataatt atatataatt atatataaaa
     4621 agctatagat aaggtcatct tatagcgact ataaaaaagt gaacaagcct agtcttctgt
     4681 acattagaat tcagatttgt tcactttttg tttaatactc aatgaaaatc aaagagcaaa
     4741 ctagaa
//
LOCUS       DAVBCG010000128         4716 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBCG010000128 DAVBCG010000000
VERSION     DAVBCG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.128"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            284..1663
                     /locus_tag="VQ495_001796"
     CDS             284..1663
                     /locus_tag="VQ495_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2531719.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            1677..2342
                     /locus_tag="VQ495_001797"
     CDS             1677..2342
                     /locus_tag="VQ495_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2531720.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            2697..3146
                     /locus_tag="VQ495_001798"
     CDS             2697..3146
                     /locus_tag="VQ495_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2531721.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            3303..3713
                     /locus_tag="VQ495_001799"
     CDS             3303..3713
                     /locus_tag="VQ495_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2531722.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQIAKSVYGFN"
     gene            3829..>4716
                     /gene="pavB"
                     /locus_tag="VQ495_001800"
     CDS             3829..>4716
                     /gene="pavB"
                     /locus_tag="VQ495_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2531723.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLV"
BASE COUNT         1320 a          893 c         1063 g         1440 t
ORIGIN      
        1 tgcaacctca aaacagtgtt ttgagcaatc tgtgactagc tttctaatcg atgccttggt
       61 tctcattgcc tataatcaaa aagagaaatt ttctcctgaa aagcatatag agtagctggc
      121 gttaaaagct cctgtcttgc ttttttgacc tatagtcaca tctatcaagt attgttcttg
      181 cctaagctat caataaaaag gtggcatttt ttaggcttgg tgttagtaga ttttgcctta
      241 tcctatctaa gtcatttcga actttttatg gtacaatgga aacatgttat tcaaattatc
      301 taaggaaaaa atagagctag gcttatctcg tttatcgcca gcccgtcgta tttttttgag
      361 ttttgccttg gtcattttac taggctctct tcttttgagc ttgccctttg tccaagttga
      421 aagctcacga gcgacttatt ttgatcatct tttcactgct gtctctgcag tctgtgtgac
      481 aggtctctca acccttccag tagctcacac ctataatata tggggccaaa taatctgttt
      541 gctcttgatt cagatcggtg gtctagggct catgaccttt attggggttt tctatatcca
      601 gagcaagcaa aagcttagtc ttcgtagccg tgcaactatt caggatagtt ttagttatgg
      661 agaaactcga tctttgagaa agtttgtcta ttctattttt ctcacgacct ttttggttga
      721 gagcttggga gctattttgc ttagttttcg ccttattcct caacttggct ggggacgtgg
      781 tctttttagt tccatttttc tagcgatctc agccttctgt aatgccggtt ttgataattt
      841 agggagcacc agtttatttg cttttcagac cgatttactg gtcaatctgg tgattgcagg
      901 cttgattatt acaggcggcc ttggttttat ggtctggttt gatttggctg gtcatgtagg
      961 aagaaagaaa aaaggacgtc tgcactttca tacgaagctt gtactattgt tgactatagg
     1021 tttgttgtta tttggaacgg caactactct ctttcttgag tggaacaatg ctggaacgat
     1081 tggcaatctc cctgttgccg ataaggtttt agttagcttt tttcaaacag tgacgatgcg
     1141 aacagctggc ttttctacga tagattatac tcaggctcat cctgtgactc ttttgattta
     1201 tatcttacag atgtttctag gtggggcacc tggaggaaca gctgggggac tcaagattac
     1261 gacatttttt gtcctcttgg tctttgcacg aagtgagctt ctaggcttgc ctcatgccaa
     1321 tgttgcgaga cgaacgattg cgccgcgaac ggttcaaaaa tcctttagtg tctttattat
     1381 ctttttgatg agcttcttga taggattgat tctgctaggg ataacagcca aaggcaatcc
     1441 tccctttatc cacctcatat ttgaaaccat ttcagctctt agtacagttg gtgtaacggc
     1501 aaatctgact cctgaccttg ggaaattggc tctcagtgtt atcatgccac ttatgtttat
     1561 gggacgaatt ggtcccttga ccttgtttgt tagcttggca gattaccatc cagaaaagaa
     1621 agatatgatt cactatatga aagcagatat tagtattggt taagaaagga aagagcatgt
     1681 cagatcgtac gattggaatt ttgggcttgg gaatttttgg gagcagtgtc ctagctgccc
     1741 tagccaagca ggatatgaat attatcgcta ttgatgacca cgcagagcgc atcaatcagt
     1801 ttgagccagt tttggcgcgt ggagtgattg gtgacatcac agatgaagaa ttattgagat
     1861 cagcagggat tgatacctgc gataccgttg tagtcgcgac aggtgaaaat ctggagtcga
     1921 gtgtgcttgc ggttatgcac tgtaagagtt tgggggtacc gactgttatt gctaaggtca
     1981 aaagtcagac cgctaagaaa gtgctagaaa agattggagc tgactcggtt atctcgccag
     2041 agtatgaaat ggggcagtct ctagcacaga ccattctttt ccataatagt gttgatgtct
     2101 ttcagttgga taaaaatgtg tctatcgtgg agatgaaaat tcctcagtct tgggcaggtc
     2161 aaagtctgag taaattagac ctccgtggca aatacaatct gaatattttg ggtttccgag
     2221 agcaggaaaa ttccccattg gatgttgaat ttggaccaga tgacctcttg aaagcagata
     2281 cctatatttt ggcagtcatc aacaaccagt atttggatac cctagtagca ttgaattcgt
     2341 aaagagggat gacccctctt ttttgatgcc caagatggca aatagagaca gaagcccctt
     2401 gtcttctagt aaaagttctt caaaggctgg actttatggt aaaatagaag gaagtgacaa
     2461 gagagagtaa tactcaatga aaatcaaaga tcaaactcgg aaactagctg cgggctgctc
     2521 aaaacactgt tttgaggttg tggatagaac tgacgaagtc agctcaaaac actgttttga
     2581 ggttgcagat agaactgacg aagtcagtaa catctatacg gcaaggcgac gttgacgcgg
     2641 tttgaagaga ttttcgaaga gtataagaaa aaatcagtcc cctaaaggag cagattatga
     2701 agttattgtc tatcgccatt tctagctata atgcagcagc ctatcttcat tactgtgtgg
     2761 agtcgctagt gattggtggt gagcaagttg ggattttgat tatcaatgac gggtctcagg
     2821 atcagactca ggaaatcgct gagtgtttag ctagcaagta tcctaatatc gttagagcca
     2881 tctatcagga aaataaatgc catggcggtg cggtcaatcg tggcttggca gaggcttctg
     2941 ggcgctattt taaagtagtt gacagtgatg actgggtgga tcctcgtgcc tacttgaaaa
     3001 ttcttgaaac cttgcaggaa cttgagagca agggtcaaga ggtggatgcc tttgtgacca
     3061 attttgtcta tgaaaaggaa ggtcagtctc gtaagaagag tatgagttac gagtcagtct
     3121 tgcctgttcg gcagattttt gactaggacc aggtcggaaa tttctccaaa ggccagtata
     3181 tcatgataca ctcgctgatt tatcggacag atttgttgcg tgctagccag ttctaactgc
     3241 ctgaacatac tttttatgtc gatgatctct ttgtctttac gccccttcag caggtcaaga
     3301 ccatgtacta tctgcctgtc gatttctatc gttatttgat tgggcgtgag gaccagtctg
     3361 tcaatgagca agtgatgatt aagcgcattg accagcaact caaggtcaat cgactcttgg
     3421 tagaccagct tgatttgtcc caagtgagtc atcctaaaat gcgagaatat ctgctgaatc
     3481 atattgaaat cacgacggtg atttccagta ccctgctcaa ccgatctgaa acagcggagc
     3541 atctggcaaa aaaacgccaa ttgtggacct atattcagca gaaaaatcca gaagtctttc
     3601 aggctattcg taagaccatg ttgagccgtt tgaccaaaca ttctgtcttg ccagatcgca
     3661 aactgtccaa tgtcgtctat caaatcgcca aatctgttta tggatttaat taatataagt
     3721 gttttataag agggatttaa gaaaaatttt aactttttct tagtcctttt taatttcagg
     3781 agattatact agagtcatca aataaagaaa gactctaagg agaatcctat gaaattcaat
     3841 ccaaatcaaa gatatactcg ttggtctatt cgccgtctca gtgtcggtgt tgcctcagtt
     3901 gttgtggcta gtggcttctt tgtcctagtt ggtcagccaa gttctgtacg tgccgatgtg
     3961 gtcaatccga ccccaggtca agtcttacct gaagagacat cgggaacgaa agagggtgac
     4021 ttatcagaaa aaccaggaga caccgttctc actcaagcga aacctgaggg cgttactgga
     4081 aatacgaatt cacttccgac acctacagaa agaactgaag tgagcgagga aacaaactct
     4141 tctagtctgg atacactttt tgaaaaagat gaagaagctc aaaaaaatcc agagctaaca
     4201 gatgtcttaa aagaaactgt agatacagct gatgtggatg ggacacaagc aagtccagca
     4261 gaaactactc ctgaacaagt aaaaggtgga gtgaaagaaa atacaaaaga cagcatcgat
     4321 gttcctgctg cttatcttga aaaagctgaa gggaaaggtc ctttcactgc cggtgtaaac
     4381 caagtaattc cttatgaact attcgctggt gatggtatgt taactcgtct attactaaaa
     4441 gcttcggata atgctccttg gtctgacaat ggtactgcta aaaatcctgc tttacctcct
     4501 cttgaaggat taacaaaagg gaaatacttc tatgaagtag acttaaatgg caatactgtt
     4561 ggtaaacaag gtcaagcttt aattgatcaa cttcgcgcta atggtactca aacttataaa
     4621 gctactgtta aagtttacgg aaataaagac ggtaaagctg acttgactaa tctagttgct
     4681 actaaaaatg tagacatcaa catcaatgga ttagtt
//
LOCUS       DAVBCG010000129         4635 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBCG010000129 DAVBCG010000000
VERSION     DAVBCG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.129"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(85..1833)
                     /locus_tag="VQ495_001801"
     CDS             complement(85..1833)
                     /locus_tag="VQ495_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531724.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1835..3559)
                     /locus_tag="VQ495_001802"
     CDS             complement(1835..3559)
                     /locus_tag="VQ495_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531725.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3617..4555)
                     /gene="msrB"
                     /locus_tag="VQ495_001803"
     CDS             complement(3617..4555)
                     /gene="msrB"
                     /locus_tag="VQ495_001803"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2531726.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
BASE COUNT         1383 a         1020 c          969 g         1263 t
ORIGIN      
        1 agtaaacaat ttttctagct attgtaacaa aaaagtcact cgaaggcaac ttttttcgtc
       61 cataaacttt caaaagagaa tggcttattc agaactaaaa gccgcagctt tttgcatttg
      121 gtaatactta ccctttctat ccatgagttc ttgatggtta ccatattcaa caatatcacc
      181 atctactaag acaagaatta aatccgcatc ctgaatggtt gacaaacggt gagcaatgat
      241 gaagcttgtg cggcccttca tgagttttgc aaaggcatcc tgtaccagca cttctgtccg
      301 tgtatcaatg gaagaagttg cctcgtctaa gataagaatc tttggaatag ccagaaagac
      361 tcgggatatg gtcaagagct gagcttggcc gacagagaga gattctccag cattttccaa
      421 cttggtatcg tatccctgtg gcaactgttg gatgaaaaag tctgcattag ctgctttggc
      481 agcagcaatt acttgctctc gactggcttc aggattgcca aaggcaatat tatcatgaat
      541 ggtcccttgt gtgagccagg tttcttgaag caccatacca aactgctgtc tcaatgatac
      601 tcgtgtataa tcataaatgg attgcccatc cagcaagata tctcccgagc taatgggata
      661 aaaacgcata aggagattga taagagttga ttttccagca cctgtcggac caacgatggc
      721 taccttacta ccagctggaa tatcgataga caactcctta atcaaaattt tttcaggatg
      781 gtagccaaaa gagacatgtt taaaggaaat agctccctta acttggtcac tggtcaagac
      841 ttccttacct gtttcagcca cttcagggct atctaagacc ccatagatac gctctacgca
      901 agccagagca ctttgcaact cagctagcac tgaagaaata tcgttaaagg gcttggtgta
      961 ttgctgaaca tagttcaaaa aagtcactaa acgaccgacg gtcaaggttg aacccatcat
     1021 gatacgataa gctcctactc cagctaaaag ggcataaatg agtgcattta caaagcgagt
     1081 cgaaggattg accgttgaag aataaaagat ggctgactga gaatagcctg agtagttgtc
     1141 atgagcctca cgcaatcttt ggataaattc tgtttgagca ttgaaggact ggattatagt
     1201 ctgctggcta agcgattctt caatcaactg agtctgaatt cccctcgtct ctgtttgctt
     1261 ctggaagaga tgataggatc tcttggcaat aaagcgtgaa atcaccatgg acagtggcgt
     1321 caacagcaag actaagagag tcatgaggag atgaatttgg agcatggcta gaatactaac
     1381 caaaatcatc aaaacaccaa tgaaaaattg gttaaaaatc atggtcaagc cagctgccaa
     1441 ctgttcgata tccgtggtta cacgactgac catctctcca ttgccttgcc gatccgcaaa
     1501 agcaatcggt aaccgatgga gtttatggat gattcgctct cgtaaatctc tggtataaga
     1561 gaagattaga cgattataga ggagaggatt ggcccattgt accagagtat ttcctattac
     1621 caccaagagc atctggagaa aaatctgcca aaaaactggt gatgaaccag ccactaggac
     1681 ttggtcaatg acctgcccaa tcagaatagg taggtaaatt gataagccaa cttgggcaat
     1741 agttcctaga aaggctagga aaaggaggaa gggatggttt gctaaatcta cgactaaacg
     1801 tttgagcgtc tggtttacag tttgtcgttt cattctagtc ctcctttcca tgttgggatg
     1861 cattgatttc acgatagact tgactggatt tcatcaagtc atcgtgcttg ccaacagcta
     1921 gcaactcacc tttttccaag aggagaatct ggtccgccat ctggagtgtc gaagtccgtt
     1981 gagaaatcaa gattaagctc atatttggca gattttcgcg gatagctttc aagagcttgg
     2041 actctgtaat ggtgtccagt gctgaggtcg catcatctag gatgagaaac ggagcctggc
     2101 gcaagactgc tcgtgcgata gacagccttt gtttttgtcc acctgagaaa tttcgccctc
     2161 ctgcttcaac tagagcatcc aagagtcctt ccttatcact gacaaaatcc ttagcttgcg
     2221 caatctccaa ggcttgccag agttcttgat cagtcacttc ttgatttaaa cctaaagtca
     2281 agttggaacg aatagttccc ttaaagagtt cgaccttttg aggtacatag gcaatccaag
     2341 accgccactg ctccaaatta agaggactac gtccattttg ataaaggtca atgttcccct
     2401 tgtctactgg ataaagtcca agtaagagtt gcaccaagct tgatttacca gaaccagtcc
     2461 ccccaatgat accaaggatt tgtccttgag tcatatcaaa ggaaatgtct ctcagagaag
     2521 gctgggccgc atcaggatag gtaaaagtca attcttggac ttgtaaaacc cgaccactgg
     2581 taacttgctt ttgttctaat tctgaatgga tgttttcggg agattccgca aagacctcct
     2641 cgattctctt ggctgagata taggactggt tgagggaatt gatcagcatg gctagcttga
     2701 ccaattccac caaaatctgt aagaggtaat tgataagagc aatgagagca ccttgactga
     2761 gtactcctcc ttgaattgaa atatagcctt gccagataat aacgagaaga gttccattga
     2821 caatcagata ggtcagaggt gttaataaac tagacaagaa acctgtcttt tcttgtaatc
     2881 tagcataaac ttggttaagg gtttgaaaaa tctgtaactc tcgtttttct tgaccaaaag
     2941 cacgaataac ccgcatccct tgcaattgct ggcgcgtttc ctgaaccagt tggtccgttt
     3001 tctttctgag actactgtag agaggattga ccaatcgaga taaccctaca atgacaatgg
     3061 tcaaaatggc aaccatgact aagaaccaga aagtcaactc agctgagatt cgataagcca
     3121 taaaaatggc accaaaaacg ataatgggcg ctcgtaaaaa gagacgcagg aattgattga
     3181 taccagtctg aatctggtag gtatccgaag tcaagcgagt aaccaaacta gaagttgtca
     3241 gacggtctct gctgtccttt ggcaaggaaa gaatatgacg ataaagatcg tttgtcaatt
     3301 ccttagcaaa acctactgct gcctttgctg agtaaaattg agctatcaag gccactaaaa
     3361 cgccaattac tgcaaagata aggagcaggc caatctgcat ccagagatga ccttgatctc
     3421 cctgaggtaa agattggtca acaatcccag caatcaccat gggaaccaag agctcaaaaa
     3481 cagcttctaa cagcttgaac aagggggcta aaattgattc cttgatgtag ggtttgaagt
     3541 aagataatag gtgtttcata aatcccttct attcatattc tagaaatgaa gaaagtggga
     3601 agccccactc tctgttttat ttgtttaagt aaggcaatag atagccatat cctgcttttt
     3661 ccatctcatc cttggccaca aagcgtaaag aagcagaatt gatacagtaa cggaggccgc
     3721 ctaactcccg cggtccatct gtgaaaacat gacccaagtg agcactgcct gaacgagaac
     3781 gaacttcaat tcgctccatt ccatggctca gatccttgta ataatgaatc aactctttgg
     3841 aaatcggacg gctaaaactt ggccaaccac aacctgaagc aaacttatcc ttggcaaaaa
     3901 agagtggctc acctgtcgta atatctacat aaatcccctc ttcaaaggtt tggtcatagg
     3961 cattggtaaa tggagcctct gtagcagctt cttgtgtgac acgataagac tcttcagata
     4021 gactggcctt caacacctct tgactaggct tttcatagtt tgctgcatca atcaatggct
     4081 tatcagcatc ggtcacatcg atatgacagt aacctgaagg attcttcctg agatagtctt
     4141 ggtggtagtc ttcagccaga atgtagtggc gtaattgctc cacttctact gcaatctttc
     4201 gacccagcat gcgttcctgc tcctgcacca ctgtgtagat agctggcaaa tctgcttcat
     4261 cctgataata aatcccagtt cgatattggc gaccacggtc attcccttgt tgattgatag
     4321 atagaggatc gataactcgg aaataataaa gtaaaatctc tctgagtgac acttccttct
     4381 catcgtaaat cacttggacc gtttctgcat ggtctgtttc cttgatcaac tggtaattgg
     4441 tcgtttcgac ttgaccatta gcgtagccaa cactggtttc tagcactcca gaaatgcgtg
     4501 aaaaatattc ctctaggccc caaaaacaac cacctgctag ataaatttct gccatcttta
     4561 tttctccttt atcacgtttt taactaatac tcttcgaaaa tctcttcaaa ccacgtcagc
     4621 gtcaccttac cgtag
//
LOCUS       DAVBCG010000130         4596 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBCG010000130 DAVBCG010000000
VERSION     DAVBCG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.130"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            537..1190
                     /gene="rr06"
                     /locus_tag="VQ495_001804"
     CDS             537..1190
                     /gene="rr06"
                     /locus_tag="VQ495_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2531727.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            1187..2527
                     /gene="hk06"
                     /locus_tag="VQ495_001805"
     CDS             1187..2527
                     /gene="hk06"
                     /locus_tag="VQ495_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2531728.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            2610..3137
                     /locus_tag="VQ495_001806"
     CDS             2610..3137
                     /locus_tag="VQ495_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2531729.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            3216..>4596
                     /locus_tag="VQ495_001807"
     CDS             3216..>4596
                     /locus_tag="VQ495_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET2531730.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGDEVESHLKKILSEIQTQLDRKRHAKTVALINKLQDIKRTYSYKLNVLEEK
                     PKAELTSKTKEELTAAFEQFKKDTLPTEPGKKVAEAEKKVAEAEKKAEDQKEEDRRNY
                     PTNTYKTLELEIAEFDVKVKEAELELLKEKAKKPLNEGTIKQAKEKVESKKAEATRLE
                     NIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAPKPENP
                     APKPEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQ
                     PSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYL
                     NANGSMATGWLQNNGSWYYLNANGSMATGWLQYNGSWYYLNANGDMATGWLQYNGSWY
                     YLNANGDMAT"
BASE COUNT         1598 a          705 c          960 g         1333 t
ORIGIN      
        1 tttgaggttg cagatagagc tgacgtggtt tgaagagatt ttcgaagagt atgaaactaa
       61 aacctatagc ttctaaacga tccgtggttt tcaccattca acacaaaatt catatgttta
      121 ttaccctccg tcgtatttgt cttagagcgt gtgtagtaga aaaagagcag tcttatctga
      181 aatttttatt ctttcaaaag agacctgttt cttttttgca tgtcaaatcc gttctagctg
      241 gtatttgaaa aatcaaacta atattcaatg aaaatcaaag aacaaactag gcagctagcc
      301 acaggttgct caaaacactg ttttgaggtt gtagatagag ctgacgtagt ttgaagagat
      361 tttcgaagag tataagctgc aagatgaatg attttcttgt attgacgttg ttgttgacaa
      421 aaagtagcgg ataaatgaaa tccattccat tatcatagat gataggctgg taggaaattt
      481 tcaaatagca tacaggaaat agatgtatgg agttctggta gtagaaaggg agagagatga
      541 acattttagt tgcagatgac gaggaaatga ttagagaagg aattgcagca tttctgacag
      601 aagagggtta tcatgtcatt atggctaagg atggacaaga ggtcttggaa aaatttcaag
      661 atctccctat ccatctcatg gtattggatt taatgatgcc taggaagagt ggttttgaag
      721 tgttaaaaga aatcaatcaa aagcacgata ttcctgtcat cgtcttgagt gctctgggag
      781 atgaaactac tcagtcacag gtatttgatc tctatgctga tgatcatgtg acaaaacctt
      841 tttctttggt actgcttgtc aagcgtatta aggcgcttat cagacgttac tacgtcatag
      901 aggatatttg gcgatatcag gatgtaacag tggattttac ctcttacaaa gcacattata
      961 aaaatgaaga aattgatctc aaaccaaagg aattactggt actaaagtgt ttgattcagc
     1021 ataaaaatca agttttaagt agagagcaga tattggaaga aatttcaaaa gatgtagctg
     1081 atttaccttg tgatagggtc gttgatgtct atattcgtac tcttcgcaaa aaattagctt
     1141 tagattgtat cgtgactgtg aaaaatgttg ggtataagat tagcttatga taaaaaatcc
     1201 taaattatta accaagtctt ttttaagaag ttttgcaatt ctaggtggtg ttggtctagt
     1261 cattcatata gctatttatt tgacctttcc tttttattat attcaactgg agggggaaaa
     1321 gtttaatgag agcgcaagag tgtttacgga gtatttaaag actaagacat ctgatgaaat
     1381 tccaagctta ctccagtctt attcaaagtc cttgaccata tctgctcacc ttaaaagaga
     1441 tattgtagat aagcgactcc ctcttgtgca tgacttggat attaaagatg gaaagctatc
     1501 aaattatatc gtgatgttag atatgtctgt tagtacagca gatggtaaac aggtaacagt
     1561 gcaatttgtt cacggggtgg atgtctacaa agaagcaaag aatattttgt ttttgtatct
     1621 tccatataca tttttggtta caattgcttt ttcctttgtt ttttcttatt tttatactaa
     1681 acgcttgctc aatcctcttt tttacatttc agaagtgact agtaaaatgc aagatttgga
     1741 tgacaatatt cgttttgatg aaagtaggaa agatgaagtt ggtgaagttg gagaagttgg
     1801 aaaacagatt aatggtatgt atgagcactt gttgaaggtt attcatgagt tggaaagtcg
     1861 taatgagcaa attgtaaaat tgcaaaatca aaaggtttcc tttgtccgtg gagcatcaca
     1921 tgagttgaaa acccctttag ccagtcttag aattatccta gagaatatgc agcataatat
     1981 tggagattac aaagatcatc caaaatatat tgcaaagagt ataaataaga ttgaccagat
     2041 gagccactta ttggaagaag tactggagtc ttctaaattc caagagtgga cagagtgtcg
     2101 tgagaccttg actgttaagc cagttttagt agatatttta tcacgttatc aagaattagc
     2161 tcattcaata ggtgttacaa ttgaaaatca attgacagat gctaccaggg tcgtcatgag
     2221 tcttagggca ttggataagg ttttgacaaa cctgattagt aatgcaatta aatattcaga
     2281 taaaaatggg cgcgtaatca tatccgagca agatggctat ctctctatca aaaatacatg
     2341 tgcgcctcta agtgaccaag aactagaaca tttatttgat atattctatc attctcaaat
     2401 cgtgacagat aaggatgaaa gttccggttt gggtctttac attgtgaata atattttaga
     2461 aagctatcaa atggactata gttttctccc ttatgaacac ggtatggaat ttaagattag
     2521 cttgtagaca gattagtttt ttattaaagt tcatataggg ttaacataag tgtgttattc
     2581 tttgtgtaga taaaagaaag gatactaata tggtattagc gattatttta gtaacattct
     2641 ttattcgatt gattttttta aagcgttcga tagagaatga gaaacgaatc cttagcaatg
     2701 gcgggaaaga atttggagtt gagaatacaa aacgattaac tatggctcat attgtttttt
     2761 atctctcttg cttggttgag gcaatggtgc acaagacaat ttttgatggc atgggcatgg
     2821 ttggtttagt cttgcttatt ttttctatgc tgatgttaat gttggtgatt cacttgttgg
     2881 gagatatttg gacagtgaag cttatgcttg tcaataatca caaatatgta gatcatatct
     2941 tgtttaggac agtaaaacat cctaattact ttttaaatat tcttcctgag ttgattggct
     3001 tgaccttgtt gagtcatgct tatgtgactt ttgttttagt ttttccagtt tatgcagtta
     3061 ttttgtatcg acgaatagct gaagaggaaa agctattaca tgaagttata atcccaaatg
     3121 gaagcataaa gagataaata caaaattcga tttatataca gttcatattg aagtgatata
     3181 gtaaggttaa agaaaaaata tagaaggaaa taaacatgtt tgcatcaaaa agcgaaagaa
     3241 aagtacatta ttcaattcgt aaatttagta ttggagtagc tagtgtagta gttgctagct
     3301 tgttcttagg aggagtagtt catgcagaag gggttagaag tggggatacc cccaaggtta
     3361 catctagtgg ggatgaagtc gagtcgcatc taaaaaaaat attgagtgag atccaaacac
     3421 aattagatag aaaacgacat gccaaaactg tagcgctaat taacaagttg caagacatta
     3481 agagaacgta ttcgtataaa ttaaatgttt tagaagagaa gccgaaagct gagttgacgt
     3541 caaaaacaaa agaagagtta accgcagctt ttgagcaatt taaaaaagat acattaccaa
     3601 cagaaccagg aaaaaaggta gcagaagctg agaagaaggt tgcagaagct gagaaaaaag
     3661 ccgaggatca aaaagaagaa gatcgtcgta actacccaac caatacttac aaaacgcttg
     3721 aacttgaaat tgctgagttc gatgtgaaag ttaaagaagc ggagcttgaa ctattaaaag
     3781 agaaagctaa gaaaccttta aacgagggca caattaagca agcaaaagag aaagttgaga
     3841 gtaaaaaagc tgaggctaca aggttagaaa acatcaagac agatcgtaaa aaagcagaag
     3901 aagaagctaa acgaaaagca gcagaagaag ataaagttaa agaaaaacca gctgaacaac
     3961 cacaaccagc gccggctcct caaccagaaa aaccagctcc aaaaccagaa aaaccagctc
     4021 cagctccaaa accagagaat ccagctccaa aaccagaaaa accagctcca gctccaaaac
     4081 cagagaatcc agctgaacaa ccaaaagcag aaaaaccagc tgatcaacaa gctgaagaag
     4141 actatgctcg tagatcagaa gaagaatata atcgcttgac tcaacagcaa ccgccaaaaa
     4201 ctgaaaaacc agcacaacca tctactccaa aaacaggctg gaaacaagaa aacggtatgt
     4261 ggtacttcta caatactgat ggttcaatgg cgacaggatg gctccaaaac aatggctcat
     4321 ggtactacct caacagcaat ggcgctatgg cgacaggatg gctccaaaac aatggttcat
     4381 ggtactatct aaacgctaat ggttcaatgg caacaggatg gctccaaaac aatggctcat
     4441 ggtactacct aaacgctaat ggttcaatgg cgacaggatg gctccaatac aatggttcat
     4501 ggtactacct aaacgctaat ggtgatatgg cgacaggatg gctccaatac aatggttcat
     4561 ggtactacct aaacgctaat ggtgatatgg cgacag
//
LOCUS       DAVBCG010000131         4579 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBCG010000131 DAVBCG010000000
VERSION     DAVBCG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.131"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            376..1311
                     /locus_tag="VQ495_001808"
     CDS             376..1311
                     /locus_tag="VQ495_001808"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2531731.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            1345..2382
                     /locus_tag="VQ495_001809"
     CDS             1345..2382
                     /locus_tag="VQ495_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2531732.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            2555..3160
                     /locus_tag="VQ495_001810"
     CDS             2555..3160
                     /locus_tag="VQ495_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2531733.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            3316..3846
                     /locus_tag="VQ495_001811"
     CDS             3316..3846
                     /locus_tag="VQ495_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2531734.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            3867..>4579
                     /gene="rlmN"
                     /locus_tag="VQ495_001812"
     CDS             3867..>4579
                     /gene="rlmN"
                     /locus_tag="VQ495_001812"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2531735.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFP"
BASE COUNT         1418 a          846 c          984 g         1331 t
ORIGIN      
        1 tcctgttctt gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc
       61 tcgtaataga taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta
      121 ttgcaaggtt ttctagagca acagagtcat ggaatggact catggttgag atttctcctt
      181 gttgcttgga cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact
      241 agctgtttcc atagcatgac ttctgtacta gactttcttt tccgaataaa tagatagaac
      301 cacagaatct agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc
      361 tggaggatta gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat
      421 tgcttgatga atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa
      481 aactcagcgg caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat
      541 cctgagccac gctaccaaga tgttccactt ggttccatca actctatggg cttgccaaat
      601 aatggcttag actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga
      661 actttcttct tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa
      721 gtccaagaga gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca
      781 ggtaaacctc agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt
      841 gcttacttca ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt
      901 gaccaagcgg cagctatttt caacaaatat ccgctcaagt ttgtcaactg tgttaactct
      961 atcggaaacg gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt
     1021 ggtggaattg gtggagaata catcaaaccg actgctctag ccaatgttca cgccttttat
     1081 caacgtttaa atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat
     1141 gcctttgaac acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa
     1201 gaaggcgtca gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa
     1261 ggctacgaga gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatcg
     1321 aaaaatctga agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta
     1381 tcaaatttac cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg
     1441 aagagtcaag tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt
     1501 gatatgaaag aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt
     1561 gcggatgaga aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc
     1621 tttttgacag atgatgagct taagtcattt gaggaatacc ttgacaattc ttctccaaca
     1681 accaagttga taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta
     1741 cttaagcgtg atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag
     1801 tatttccaaa agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat
     1861 ctcctcatca agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag
     1921 tcctataagg cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc
     1981 ttgcaggaca atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg
     2041 cgcgatttgg tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc
     2101 atgctgggac aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca
     2161 gaatcgcaga ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa
     2221 atcaagtttg cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt
     2281 tcctatttga ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt
     2341 tttgaaaagg cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa
     2401 cccgcgtcag gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt
     2461 atgtcagtga tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat
     2521 cattttcata tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc
     2581 atatgcatac gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga
     2641 caaacaccat caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat
     2701 cggtgaagac cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca
     2761 agcacttatc aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac
     2821 tcccgagaaa acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc
     2881 atttgaagaa ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg
     2941 ggcatggttg gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga
     3001 cacaccaatc tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta
     3061 ctacgtgaaa taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa
     3121 ctggaataaa gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat
     3181 tgttcttctc tttttaggtt ataagattct ggtctgacaa aatcgtcaga cttttttcat
     3241 ttttatgaga aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt aagtaatcgt
     3301 ggaaaaagga tatctatgcg aaaagaaatt gcacctgaat tatacaacta taacaagttt
     3361 cctggtccgg agttccattt acacggggat aaggtcgaaa cggaagggat agctttttcc
     3421 ttggttgaaa atatcaagga tgcctttgat gtgacgactt ttaatcagcg tttttcagaa
     3481 gtcttaacca agtttgatta tatcgtgggg gactggagca acgaacagct tcgcctacga
     3541 ggtttttaca aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg tttacaagac
     3601 taccttttag agtattgtag ttatggttgt gcctattttg tcttagaaaa tgaagcccct
     3661 aagcgagcat catttgacaa gaaaatgcgt aagacggaag aagaaacacc ttctagaaaa
     3721 ggaaagaaac cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa tagacgtcgt
     3781 cagaaagacc agcattctca gaaagaggac aaggaacaac gtcattttgt cattcgtcag
     3841 aagtgagtag agaataagga gaagaaatga aaccctcaat tcatagtttg gcacatcaaa
     3901 ccatgcagga atgggtattg gagcagggag aaaagaaatt ccgtgcagat caaatctggg
     3961 aatggctcta ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt tccaaggatt
     4021 tgattgctaa gctcaatgac cagtttgtgg tcaatccctt gaaacaaggt attgtgcaag
     4081 agtctgctga tggtacagtc aaatatctct ttgaattgcc tgacggtatg ttaattgaga
     4141 ctgtactcat gtgtcaacac tatggtttat ctgtctgtgt gaccactcag gtcggctgta
     4201 atatcggttg taccttctgt gcctctggtt tgattaagaa gcagcgtgac ctcaataacg
     4261 gtgaaatcgt agcgcaaatc atgctggttc agaaatactt tgatgagcgt ggtcaggatg
     4321 aacgcatcag ccatatcgtt gtcatgggaa tcggtgagcc ctttgataat tacaacaatg
     4381 tcttgaattt ctttcgtact atcaatgatg ataaaggaat ggctatcggt gctcgtcaca
     4441 tcacggtttc aatctcaggt ttggctcata aaattcgtga ctttgctgat gaaggggttc
     4501 aggtcaatct tgccgtgtct cttcacgcac ccaacaatga acttcgttca agtatcatga
     4561 agattaaccg tgcctttcc
//
LOCUS       DAVBCG010000132         4575 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBCG010000132 DAVBCG010000000
VERSION     DAVBCG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.132"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            157..1416
                     /locus_tag="VQ495_001813"
     CDS             157..1416
                     /locus_tag="VQ495_001813"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2531736.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            1432..1983
                     /locus_tag="VQ495_001814"
     CDS             1432..1983
                     /locus_tag="VQ495_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531737.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            1976..3253
                     /locus_tag="VQ495_001815"
     CDS             1976..3253
                     /locus_tag="VQ495_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2531738.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3269..4129
                     /locus_tag="VQ495_001816"
     CDS             3269..4129
                     /locus_tag="VQ495_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2531739.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            4352..4462
                     /locus_tag="VQ495_001817"
                     /pseudo
     CDS             4352..4462
                     /locus_tag="VQ495_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT         1373 a          738 c         1051 g         1413 t
ORIGIN      
        1 tctttgattt tcattgagta ttagtaattc agttactaac tcgtcaactc tgatttatcc
       61 aataaaattg aaaaggatgg aaaaaaggat aaatttatga tatactttat tttgaagacc
      121 ttattagaaa tcttgaaaga gtattgaaaa cttagaatga gaaaaattgt tatcaatggt
      181 ggattaccac tgcaaggtga aatcactatt agtggtgcta aaaatagtgt cgttgcctta
      241 attccagcta ttatcttggc tgatgatgtg gtgactttgg attgcgttcc agatatttcg
      301 gatgtagcca gtcttgtcga aatcatggaa ttgatgggag ctactgttaa gcgttatgac
      361 gatgtattgg agattgaccc aagaggtgtt caaaatattc caatgcctta tggtaaaatt
      421 aacagtcttc gtgcatctta ctatttttat gggagcctct taggccgttt tggtgaagcg
      481 acagttggtc taccgggagg atgtgatctt ggtcctcgtc cgattgactt acaccttaag
      541 gcgtttgaag ctatgggtgc cactgctagc tacgagggag ataacatgaa gttatctgct
      601 aaagatacag gacttcatgg tgcaagtatt tacatggata cggttagtgt gggagcaacg
      661 attaatacga tgattgctgc ggttaaagca aatggtcgta ctattattga aaatgcagcc
      721 cgtgaacctg agattattga tgtagctact ctcttgaata atatgggtgc ccatatccgt
      781 ggggcaggaa ctaatatcat cattattgat ggtgttgaaa gattacatgg gacacgtcat
      841 caggtgattc cagaccgtat tgaagctgga acatatatat ctttagctgc tgcagttggt
      901 aaaggaattc gtataaataa tgttctttac gaacacctgg aagggtttat tgctaagttg
      961 gaagaaatgg gagtgagaat gactgtatct gaggacagca tttttgtcga ggaacagtct
     1021 aatttgaaag caatcaatat taagacagct ccttacccag gctttgcaac tgatttgcaa
     1081 caaccgctta cccctctttt actaagagcg aatggtcgtg gtacaattgt cgatacgatt
     1141 tacgaaaaac gtgtaaatca tgtttttgaa ctagcaaaga tggatgcgga tatttcgaca
     1201 acaaatggtc atattttgta cacgggtgga cgtgatttac gtggggccag tgttaaagcg
     1261 accgacttaa gagctggggc tgcactagtc attgctgggc ttatggctga aggtaaaact
     1321 gaaattacca atatcgagtt tatcttacgt ggttattctg atattatcga aaaattacgt
     1381 aatttaggag cggatattag acttgttgag gattaaaccg tagaggtgtt tatgaatatt
     1441 tggaccaaat tagcaatgtt ttcttttttt gaaacggatc gcttgtattt gcgtcctttc
     1501 ttttttagtg atagtcagga cttccgcgag atagcttcaa atccagaaaa tcttcaattt
     1561 attttcccaa cgcaggcaag tctggaagaa agtcaatatg cactggccaa ttactttatg
     1621 aagtcccctt tgggagtgtg ggcaatttgt gaccagaaaa atcaacaaat gattggttct
     1681 attaaatttg agaagttaga tgaaatcaaa aaagaagctg agcttggcta ttttttgaga
     1741 aaagatgctt ggtcgcaagg atttatgaca gaggttgtta gaaaaatttg tcagctttct
     1801 tttgaggaat ttggcttaaa acaattattt atcattaccc accttgaaaa tgaagctagc
     1861 caaagagttg ctcttaagtc tggatttagt ttgttccgtc agtttaaggg aagtgatcgt
     1921 tacacaagaa aaatgcggga ttatcttgaa tttcggtatg taaaaggaga gttcaatgag
     1981 taagcatcag gaaattctaa gctatttgga ggaattacca gtaggtaaaa gggtcagtgt
     2041 tcgtagcatt tcgaatcatc taggagttag tgatggaaca gcctatcgag ctattaaaga
     2101 agctgaaaac cgtggaattg tggagacccg tcctagaagt ggaacaattc gtgttaaatc
     2161 ccagaaagtt gctatagata gattaacgtt tgctgaaatt gcagaagtga cttcttctga
     2221 ggttctggct gggcaagaag gtttagagag agaatttagt aagttttcaa ttggtgccat
     2281 gactgaacaa aatatcttgt cttaccttca tgatgggggg ctcttgattg tcggagaccg
     2341 aacccgtatt cagttgctag ccttggaaaa tgaaaatgca gttctggtta cagggggatt
     2401 tcaggttcat gatgatgtgc ttaaactggc caatcaaaaa gggattcctg ttctaagaag
     2461 taagcatgat acctttaccg tcgctaccat gatcaataaa gccttgtcaa atgtccaaat
     2521 caagactgat attctgacag ttgagaaact ttatcgccct agtcatgagt atggttttct
     2581 gagagagaca gatacagtta aagattattt ggacttggtt cgtaagaatc gtagcagccg
     2641 tttccctgtt atcaatcaac atcaggtcgt tgttggcgtt gtaaccatga gagacgctgg
     2701 tgataaatca ccaagcacga caattgataa ggttatgtct cgtagtctat ttttggttgg
     2761 attatcgaca aatattgcca atgtgagtca acggatgatc gcagaagact ttgaaatggt
     2821 accagttgtt cgaagcaatc aaactttgct tggcgttgtg acgcgacgag atgtcatgga
     2881 gaagatgagc cgttcccaag tttcggctct accaactttt tctgagcaga ttggacaaaa
     2941 gctctcttat caccacgatg aagtagtcat tacagtggaa ccctttatgc tagaaaaaaa
     3001 tggagttttg gctaatggtg tattggcaga aattctgacc cacatgaccc aagatttagt
     3061 tgttaatagt ggtcgcaatc tcattatcga gcagatgctg atctactttt tgcaggccgt
     3121 tcagatagat gatatattgc gcattcaggc acggattatt catcatacga gacggtcagc
     3181 tataattgat tacgatattt atcatggtca ccagattgtt tcaaaagcaa atgtgactgt
     3241 taaaattaat tagaaactag gagaaaagat gataacatta aaatcagctc gtgaaatcga
     3301 agctatggac aaggctggtg attttctagc aagtattcat ataggcttac gtgatttgat
     3361 taagccaggc gtagatatgt gggaagttga agaatatgtc cgccgtcgtt gtaaagaaga
     3421 aaatttcctt ccacttcaga ttggggttga cggtgccatg atggactatc cttatgctac
     3481 ctgttgctct cttaacgatg aagtggctca cgctttccct cgtcattata tcttgaaaga
     3541 tggtgatttg ctcaaagttg atatggtttt gggaggtccc attgctaaat ctgacctaaa
     3601 tgtctcaaaa ttaaacttca acaatgttga acaaatgaaa aaatacactc agagttattc
     3661 tggtggttta gcagactcat gttgggctta tgctgttggt acaccgtccg aagaagtcaa
     3721 aaacttgatg gatgtaacca aagaagctat gtacaagggt attgagcaag ctgttgttgg
     3781 aaatcgtatc ggtgatatcg gtgcggctat tcaagaatac gctgaaagtc gtggttacgg
     3841 tgtagtgcgt gatttggttg gtcatggtgt tggcccaact atgcacgaag aaccaatggt
     3901 tcctaactat ggtattgcag gtcgtggact ccgtcttcgt gaaggaatgg tcttaaccat
     3961 tgaaccaatg atcaatacag gcgattggga aattgataca gatatgaaaa ctggttgggc
     4021 gcataagacc attgacggtg gattgtcatg tcagtatgaa caccaatttg tcattacgaa
     4081 agatggacct gttatcttga ctagccaagg tgaagaagga acttattaat aaaaagtgaa
     4141 aagactactg gaagtttatt atgaaaaaat ccagtagatc ttttcataat aaaacgcatt
     4201 gtatcaagtg ttaggggctg atatcatgcg tttttctgct tttaagattt tttccaactc
     4261 tgtttgtaag cgcatcataa caaagggtct aggattcagg gctctcctcc tatatactat
     4321 tagtaaagta aaactaaggg aggatatttt agtgtcgcag tctattgttc ctgtagagat
     4381 tccacaatat tgtcgttttg attctaaaaa gagaaatgga attctgttta atgttcgtat
     4441 tgccaatctt aaatttacct tttttagatt agacttcctg cgaaacaaaa catggtatag
     4501 tagttctatg aatgatgaag caagtaaaca actaactgat gcacgattta agcgtcttgt
     4561 tggtgttcaa cgcac
//
LOCUS       DAVBCG010000133         4556 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBCG010000133 DAVBCG010000000
VERSION     DAVBCG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.133"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            63..227
                     /locus_tag="VQ495_001818"
     CDS             63..227
                     /locus_tag="VQ495_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2531740.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            306..1034
                     /gene="yaaA"
                     /locus_tag="VQ495_001819"
     CDS             306..1034
                     /gene="yaaA"
                     /locus_tag="VQ495_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2531741.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1085..2569)
                     /locus_tag="VQ495_001820"
     CDS             complement(1085..2569)
                     /locus_tag="VQ495_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2531742.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEGYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            2785..>4556
                     /locus_tag="VQ495_001821"
     CDS             2785..>4556
                     /locus_tag="VQ495_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2531743.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVRN
                     RVDFSIRYSGPGEFVNNNFILDKGNGFGEPSRATVSTPKLRDQVKNISKGGNFVSHSG
                     YSLEAAVETSTNQTIKFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDG"
BASE COUNT         1552 a          749 c          910 g         1345 t
ORIGIN      
        1 aaggtttatt taataaatat atgttataat aaggtaaatt aagcctaatg gaggacaaag
       61 aaatgggttt ttatttgatg gttgcttcaa tgctactcgg actgctcgct ttgaagatag
      121 gtttttctca gttcaaggag aaaaaagata aattcttatc tatcttaaca agtttagctg
      181 gcacagccct tgttctcgtg gctgtctggt taggatggcc caaataaata gaaaaacctc
      241 ggtcaaaccg aggtttttca aatgaatttc ttggttgtgg ctaaaaaata tgctacacta
      301 tcaatatgaa aattttaatc ccaacagcaa aagaaatgaa cacagacttg ccaagtatcg
      361 aggcaattcc tttaaaacca gaaagtcaga ccgtgcttga tgccttggct ctctattctg
      421 ccagtcaatt ggagagtttc tacaaggtat cagctgagaa agcggcggaa gaatttcaaa
      481 atatccaagc tttgaaaagg caaactgctc aacactatcc agccttgaaa ctttttgatg
      541 ggcttatgta ccgcaacatt aagagagata agctgaccga ggcggaacaa gattatcttg
      601 aaaatcatgt cttcattacc tcggctttgt acggtgttgt tccagccttg tcacccatgg
      661 ctcctcaccg tttggatttt ttgatgaaat taaaagtcgc tggtaagact ttgaagagcc
      721 attggaaggc agtctatgat gaagctctga agaaggaaga agtgattttc tctctcttgt
      781 catcagagtt tgagactgta ttttctaagg aaatcagagc aaagatggtg accttcaaat
      841 tcatggagga tagaggcggt cagctgaaga ttcactcaac tatctccaag aaagcgcgcg
      901 gggcctttct aacagcttta atagaaaatc aagtacaaac tgtgggggaa gcacgtcgct
      961 tgaactttgc tggatttgtt taccgagaag atttgtcaca accacaggga ttggtttttg
     1021 taaaggaagt atagaaatca gataagagaa aagtcagcac gattatgctg acttttttga
     1081 taacttataa agtattggac aaatgaatct catttgataa ggtgttaaga tgattaacca
     1141 aattcatgat tgacagatga tttcggcaaa taaacttctt ggtaatccct gaaatataga
     1201 aattagacac gatgatatcg taatctgtct gatttaaaat ctcaggactt gtttttaatt
     1261 catcccaaat atcgaaggta aagcggttat tacagtaata ggaaagcata tcaacaaagg
     1321 taagggatat agcatggtca aagttactga taatcaaaac cttgataggt ggtcgatttt
     1381 ctaacaactg agtagagagg ttttcagcat gggttaagat ggtgtacatc aagtgttcca
     1441 actgttcagg atggtcatgc tgtccaattg cctgacggta ttgagccagt tcttgacgcg
     1501 cacttcccat aaagtctggg aagtaacctt caaatttttt aatcgtcaat gctttttgtt
     1561 caaataagat tggggtagaa aagatttcct gtctttcaaa gaaagcagtg ttgtgtaaat
     1621 gccaaatcag ctcatcgtgg ttagtaaaag taatcttata ttgttttgat aggcgttcta
     1681 atatttgact aagtaattgg taagagtatc ttgcttgact gttatcttct aaagagttga
     1741 agaattcttg aggatctagg aaaatatcat tctgaagaaa agaaataaaa atttgagcga
     1801 ttgtatctgg agtcatttca agcccaaaat gtttagagaa ataagcaagt gtctcctgga
     1861 aatttggaat gctctttaaa agaggataat attctttgta aaagtggttg gggaggtcaa
     1921 tgaagtgacc atttttaacc ctgtggatac tgatagctat catcagtttg tacattctat
     1981 aaattgaaaa tcgcattgga taattcgtga ttttatagaa gaagtcagca aactctgtta
     2041 aatcttcctc aggaaggtca ggaaaaggcc aatctaggaa ataatagcgc tcagaaaagt
     2101 attgggcaaa aaagtaacga atatcgatct catttccacg tatctcggat ggagtaaaag
     2161 ataattcaat ttgaaactga gatgaaagaa cctttgtaat atttctgccc agtcgataga
     2221 ggttggcact actaaagtaa agattttcta ttgttcgata aataggtagc ccctcattga
     2281 agaacatata ctctagtaat tgaaaagatt gagaattagc aagaaaatat tgataaatat
     2341 cttctacact agtgttaact tctaaatcta tcatgattcc gttaacagaa gattggatgt
     2401 taatagatgg aaaggcaata cgtaattcat ttaaatcact ttttaagatt cgttctgtac
     2461 atcctaattt ttcggctagt tcatgtaatt tcatccaatt acgattctga attagggttt
     2521 ccattaagcg taattgtctt tgaacttttg tggataaaag gtttctcata cgggtatttc
     2581 ctccataatt cattatatct ttttttgtct aagtagtata aaaaaattag agcaagaaaa
     2641 ttgtcgttag taatcggaaa aattagaata atataagtgg cttttaagtg caaaaacaga
     2701 atatattaat tgttcagaaa atattgatta atgcctcaaa tagttgtaca ttgtaaacta
     2761 taaaatacta tctaggagtt taaaatgaaa cattcacata aaaaatcatt tgactggtat
     2821 agcatgcaac aacgttattc tattcgtaag tattactttg gtgcagttag tgtcttgctc
     2881 ggtaccgctt tggtattagg tgcagcagct agtgtccaaa cagtacaagc ggaagaaaac
     2941 aaacaagaaa ctaccaatag tatttctgtt ggtaggggag aagcagctac taaaccagca
     3001 gaggtttctg cgtctaataa agagaaaacc tatgcagctc caactgtagc taatccagta
     3061 gaaacgactc cagttaaaac tggagaagtt actaaaccag cagaaaaagt tgaagaagca
     3121 aaagacaaaa aagaggaagt aacgcatcaa gatgctattg acaagtcaaa attgttaacg
     3181 gctctttcgc gtgctaaaaa gttagaaagc aagttatata cagaagcaag tgctgctaac
     3241 ttgcaaacaa gtatccaagc tggtcaaagc ttgcttggga aagcagatgc atctgaagct
     3301 gaattatcag cagcagagtc atctattcaa tcatctatta ttggtctaga acttcgttct
     3361 aactctaaca aaggaactgt atcagaaact cctgtagcga agaaagctaa tatagttgaa
     3421 gcgaaggaag agactaatcc agctgcaaca actgatcgtt cagctgttga tagtgctgtt
     3481 ttgccaacta gcacagctgc caaagtagaa acaacttcag ctccagcatc tattaatgaa
     3541 atcttgaaac caggtttgag cctttctgat gctcgccaaa atccagctat ccgtaaggaa
     3601 gatgttgata gagggtatag tggttttaga gcggcgcctg ctccaacgaa ccaaccagta
     3661 actaatctag gaaataatac tgttttcact gatattagca gaggttctca tactttccgt
     3721 ggtaatggaa attcacgtcg tggaaacccg attcattttg atgtaacaac aactagagtt
     3781 agaaatagag tagatttctc aatcagatat tcaggaccag gtgagtttgt taataacaac
     3841 tttatcttag acaaaggaaa tggattcgga gaaccttctc gtgcaactgt ttcaacacca
     3901 aaattaagag accaagttaa aaacattagt aagggtggaa actttgtttc tcattcaggc
     3961 tacagtttag aagctgcagt agaaacaagc accaatcaaa caattaagtt tagtttacct
     4021 atagccaacc ctaatggtga tctgtctgtt cgattaagac ctgttacttt taatgtaggt
     4081 caaggggaat caggatctgc aactagtaat gatccatata gtaactctaa ctattacttc
     4141 agagaaaaac cactcctctt ggatgcaaat ccaaatggtg gtactaataa taagactgtt
     4201 tcagaagaca ttgacttcca aactgtctat cttccaacta gtaagttacc agaaggtcaa
     4261 actagattag ttcgagaagg tgaaaaagga caacgtcaaa tcatctataa agttcatcaa
     4321 ttgggtaacg aaacaatcct aggattgcca attagtaatt cagttattaa agaagttaag
     4381 ccacgtatta tgcaaattgg tgtggctaaa gatctaatcg atacagtaaa accacgtgtt
     4441 gatcaaaata aagtcggtga tacaaataac ctcactttct atcttgataa cgatggaaac
     4501 ggtgtttata ctgagggcgt agacgaactt gttcaaaaaa ttgctattaa agatgg
//
LOCUS       DAVBCG010000134         4438 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBCG010000134 DAVBCG010000000
VERSION     DAVBCG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.134"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..119
                     /locus_tag="VQ495_001822"
     CDS             <1..119
                     /locus_tag="VQ495_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2531744.1"
                     /translation="QQGFTDRLVGYSSSIALVFTTLIGMISLVQRRVLKEDD"
     gene            121..966
                     /locus_tag="VQ495_001823"
     CDS             121..966
                     /locus_tag="VQ495_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2531745.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            976..2295
                     /locus_tag="VQ495_001824"
     CDS             976..2295
                     /locus_tag="VQ495_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2531746.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(2503..2575)
                     /locus_tag="VQ495_001825"
     tRNA            complement(2503..2575)
                     /locus_tag="VQ495_001825"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2540..2542),aa:Lys,seq:ctt)
     gene            complement(2839..4110)
                     /locus_tag="VQ495_001826"
     CDS             complement(2839..4110)
                     /locus_tag="VQ495_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2531747.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         1294 a          809 c          976 g         1359 t
ORIGIN      
        1 atcaacaagg ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta
       61 caacgttaat tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag
      121 atgaaacgat ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga
      181 ctcttccttt ttcctatggt ttggatggta gtttcagcta tgaagccaga agcggatgtt
      241 tataaaaatt tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt
      301 aaaccttatc aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt
      361 ttttatgctg gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct
      421 tttgcaaaag tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt
      481 attattccag tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg
      541 gtcaataatc gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta
      601 tttaggaatt tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga
      661 gcaagtatcg ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct
      721 acagttggtg ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt
      781 ttgacagatt cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa
      841 ccagtttata tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg
      901 gtctatgtag tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata
      961 aaataggata tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata
     1021 gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag
     1081 attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata ccatctcttt
     1141 tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca tgctaaaagt
     1201 aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca agattatgac
     1261 cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac
     1321 actggacata tcgaagaaga aaccggtgtc cgccaagtgc aaaatatggc attttcagat
     1381 gacgggattc actttgaaaa gatttcccaa aatccagttg caactggatc agacttgcca
     1441 gatgagttga ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat
     1501 tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc
     1561 gataacctag tagaatggca gttcgaatcc atctttttaa aaggaggaga acaccaaggc
     1621 tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca
     1681 cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc
     1741 acgggtaagg tagattggag agaaaaacgt tttatcccag aatcagttca agaaattgat
     1801 catggccaag acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg
     1861 attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga acacaaatgg
     1921 gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact aagacaattc
     1981 cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga
     2041 aatgatacag attatcttga atttggttat gacagtaatg cgcagcaagt ttacattgat
     2101 cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat
     2161 gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt
     2221 gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta
     2281 tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata
     2341 gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat
     2401 tggtgttgga ttatgattta agctagtttt ctaaagaatt tgaaaaaaat tttatttaag
     2461 caaaaaaacc ttggttccaa ggcttttcct gttgtattta gatgccccct acagggatcg
     2521 aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag gaggcaaaga
     2581 aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa ttaagcaggt
     2641 gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc tgttagaaga
     2701 cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg aagtcgtaca
     2761 ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag aggaaagtgc
     2821 aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct ttctttgagc
     2881 caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat tacgaatttt
     2941 ttctggcaga tagtcttgct tgatccaatt tccaggatag ttgtgtggat agagatagtc
     3001 ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt gtcgcggaat
     3061 aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta cataggctga
     3121 gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa tgcgggcttc
     3181 ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct gggcctcagg
     3241 attggctaag ccaatatctt cataggcgat aacagtcaag cgacgagcga gactaggcag
     3301 atccccagcc tcaatcaagc gggcagcata gtggagactg gcatccacat ctgagccacg
     3361 gatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct tgtccatagt
     3421 gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc gaatgccctc
     3481 gtcattctca ggggtagaga gaacagccaa gtccagtgag ttaaaggcag agcgaaggtc
     3541 tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta ttggaaaatc
     3601 aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt cttggttaga
     3661 cagaggttcc aactcgaaaa tttgaacacg gctacgaatg gcaggagtga cagagaagaa
     3721 aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga gaggcaagag
     3781 gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga cgagaccacc
     3841 agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa ctgtcgcatt
     3901 gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac tggttttgcc
     3961 gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca ccatgcggcg
     4021 gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga tggttttagg
     4081 gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg atttgagtag
     4141 aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt gatatttcaa
     4201 tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat ttaatgagga
     4261 gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata gttgagccta
     4321 aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta tgaactataa
     4381 tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt ttgatttt
//
LOCUS       DAVBCG010000135         4416 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBCG010000135 DAVBCG010000000
VERSION     DAVBCG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.135"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..179
                     /locus_tag="VQ495_001827"
     CDS             <1..179
                     /locus_tag="VQ495_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein D"
                     /protein_id="HET2531748.1"
                     /translation="TGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNT
                     TVDGYKVNANGEWV"
     gene            580..1701
                     /gene="mnmA"
                     /locus_tag="VQ495_001828"
     CDS             580..1701
                     /gene="mnmA"
                     /locus_tag="VQ495_001828"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2531749.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1842..2297
                     /locus_tag="VQ495_001829"
     CDS             1842..2297
                     /locus_tag="VQ495_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2531750.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2307..4220
                     /gene="mnmG"
                     /locus_tag="VQ495_001830"
     CDS             2307..4220
                     /gene="mnmG"
                     /locus_tag="VQ495_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2531751.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1275 a          893 c         1077 g         1171 t
ORIGIN      
        1 caacaggttg ggtgaaagat ggagatacct ggtactatct tgaagcatca ggtgctatga
       61 aagcaagcca atggttcaaa gtatcagata aatggtacta tgtcaatggt ttaggtgccc
      121 ttgcagtcaa cacaactgta gatggctata aagtcaatgc caatggtgaa tgggtttaag
      181 ccgattaaat taaatcatgt taagaacatt tgacatttta attttgaaac aaagataagg
      241 ttcgattgaa tagatttatg ttcgtattct ttaggtacct atcttatgat ttcaggaaat
      301 gtcattaaaa aaacgactcc ttttctctaa cttgaaaaat agattagaga aaatgggttg
      361 ttttatttat tatagttatt tgaatgaaga taagaagaag gtatactcac atcattcaca
      421 taatctgtat gttgactata agtttaaaaa aacaattttt aagctcttcc ttgtcttctc
      481 caaccaagcg tgttataatg aatactgctc aagcgacctt caatcgtgaa gcacacacga
      541 ccttcaatcg tgaataaacg aatagatggg agacttacca tgagtgataa ctctaaaaca
      601 cgtgttgtcg tggggatgag tggtggtgtt gattcgtcgg tgacggctct tttgctcaag
      661 gagcagggct acgatgtgat cggtatcttc atgaagaact gggatgacac agatgaaaac
      721 ggcgtctgta cggcgaccga agattacaag gatgtggttg cggtggcaga ccagattggc
      781 attccctact actctgtcaa ttttgaaaaa gagtactggg accgcgtttt tgagtatttc
      841 ctagcggaat accgtgcagg gcgcacgcca aatccggacg ttatgtgcaa caaggaaatc
      901 aagttcaagg cctttttgga ctatgccatg accttggggg cagactatgt agcgactggg
      961 cattatgctc gagtggcgcg tgatgaggac ggcactgttc acatgcttcg tggcgtggac
     1021 aatggcaagg accagaccta tttcctcagc caactttcgc aagaacaact ccaaaaaacc
     1081 atgttccctc tgggacattt ggaaaagcct gaagtacgca gactagcaga agaagcaggc
     1141 ctttcgactg ctaagaagaa agactcgaca gggatttgct ttatcggaga aaagaacttt
     1201 aaaaactttc tcagcaacta cctgccagct cagcctggtc gcatgatgac tgtggatggt
     1261 cgcgatatgg gcgagcatgc aggtcttatg tactatacaa tcggtcagcg tggcggactc
     1321 ggtatcggtg ggcaacacgg cggtgacaat gccccttggt tcgttgtcgg aaaagatcta
     1381 agcaagaata ttctctatgt aggacaagga ttctaccatg attcgctcat gtcaactagc
     1441 ctagaagcca gtcaagtcca ctttactcgt gaaatgccag aagagtttac gctagaatgt
     1501 acggctaaat tccgttaccg tcagcctgac tctaaggtga ccgtccatgt caaaggagac
     1561 aaggcagaag ttatctttgc ggaaccacaa cgcgcgatta caccaggaca ggcagttgtc
     1621 ttttacgatg gcgaagagtg tctaggtggc ggtttgattg acaatgctta ccgcgatgga
     1681 caagtttgtc agtacattta gattgacaaa ttttctcaat ttgctacaat aataacagca
     1741 atagaaatga tggtcaaagc tcatggatgt tgcaggcttt tttgtcctgc acttctttgt
     1801 agttttgact gtttttgtgt cgtttaaggg aaaggacaag aatgactcaa caagactttc
     1861 ggacaaaagt agacaatacg gtttttggtg ttcgggctac agccttgatt gtccaaaatc
     1921 acaagcttct agttaccaaa gacaagggca agtattacac tattggaggt gcgattcaag
     1981 tcaatgaaag cacggaagac gcggtagtcc gtgaagtgaa ggaagaactg gatgtcaaag
     2041 ctcaagctgg gcagctagct tttgtggttg aaaatcgttt tgaagtggac ggcgtttcct
     2101 atcacaatat cgagtttcat tatctggtgg atttgcttga agatgccccg ttgaccatgc
     2161 aggaagatga gaaaaggcag ccctgtgagt ggattgactt ggataagctc cagaatatcc
     2221 agctagttcc agccttttta aaaacagccc taccagattg ggaaggccaa ctaagacaca
     2281 ttcatcttga ggaataggag agaaacatga cttatcattt tactgaagaa tacgatatta
     2341 ttgtaattgg tgcgggacac gctggggttg aggcttcctt ggccgctagc cgtatgggct
     2401 gtaaggtcct gcttgcgacc atcaatattg aaatgctggc tttcatgcct tgtaatccct
     2461 ctatcggtgg ttctgctaag gggattgtcg tacgtgaagt cgatgccctc ggtggcgaga
     2521 tggccaagac cattgacaag acttacatcc agatgaagat gctcaacaca gggaagggac
     2581 cagccgttcg tgcccttcgt gcgcaggctg ataaggaact ttactctaag gaaatgcgca
     2641 agacagttga aaatcaagaa aatctgaccc ttcgtcaaac catgattgat gagattttgg
     2701 tggaagatgg caaggttgtc ggtgtgcgta cagccaccca tcaagaatat gctgctaagg
     2761 ctgttattgt gacgacaggg actgctctcc gtggggaaat tatcatcgga gacctcaagt
     2821 actcatcagg tcctaaccac agcttggctt ctattaacct agctgacaat ctcaaggaac
     2881 tgggtctcga aatcggtcgt ttcaagacag gaacccctcc acgtgtcaag gcttcttcta
     2941 tcaattacga tgtgacggaa attcagccag gagacgaagt gcctaatcat ttctcataca
     3001 cttcacgtga tgaggattat gtcaaagatc aagtgccatg ctggttgacc tataccaatg
     3061 gtaccagtca tgagattatc caaaacaacc tccaccgtgc gcctatgttt acaggtgtgg
     3121 tcaagggagt ggggcctcgt tactgtccgt cgattgaaga caagattgtg cgctttgcgg
     3181 acaaggaacg tcaccaactc ttccttgagc cagaagggcg caatactgag gaagtctatg
     3241 ttcaaggact ttcaaccagt ctgcctgagg atgtccagcg tgacttggtt cattccatca
     3301 aaggtttgga aaatgcagag atgatgcgga caggttatgc tattgagtat gatatggtct
     3361 tgcctcatca gttgcgtgcg actttggaaa ccaagaaaat ctcaggtctc ttcactgctg
     3421 gtcagacaaa tggaacatca ggttatgaag aagctgctgg ccaagggatt atcgcgggta
     3481 tcaatgcggc tctgaaaatc caaggtaaac ctgagttgat tctaaaacga agtgacggtt
     3541 atatcggggt gatgatcgac gacttggtga ccaagggaac cattgaacct taccgtctct
     3601 tgaccagtcg tgctgaatac cgtctcattc ttcgtcatga caatgctgat atgcgcttga
     3661 ctgagatggg acgcgagatt ggccttgtgg atgatgaacg ctgggctcgt tttgaaatca
     3721 agaaaaatca atttgataat gagatgaaac gcctagacag tatcaaactc aagccagtca
     3781 aggaaaccaa tgctaaggtt gaggaaatgg gcttcaagcc gttgacagat gcggtgacag
     3841 ccaaggaatt ccttcgccgt ccagaagttt cttaccaaga tgtggtggcc ttcatcggac
     3901 cagctgcaga agacttggat gacaagatta tcgaattgat tgaaacagaa atcaagtacg
     3961 aaggctatat ttccaaagcc atggatcagg ttgctaagat gaaacgtatg gaagaaaaac
     4021 gcattccagc caatatcgac tgggatgaca tcgattctat tgcgacggaa gctcgtcaga
     4081 agttcaaact catcaatcca gaaaccatcg gccaagccag ccgtatttcg ggagtaaacc
     4141 cagcagatat ttctattttg atggtgtatc tggaaggtaa aaatcgtagt atttctaaaa
     4201 ctcttcaaaa atcaaaatga tacgtcgtcg gcttcttacg aatgagttca aagcttggct
     4261 ttgattcatc tccaacctcc catagttccc cgaactatgg gagctaactc aagttatgca
     4321 ttttcctagc cttctggtct gattttcatt gagttttaat ctcaaaagaa agcatagaga
     4381 aaaacagctc cgaagacggg gctgttttgt tgactt
//
LOCUS       DAVBCG010000136         4403 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBCG010000136 DAVBCG010000000
VERSION     DAVBCG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.136"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(56..289)
                     /locus_tag="VQ495_001831"
     CDS             complement(56..289)
                     /locus_tag="VQ495_001831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531752.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(282..398)
                     /locus_tag="VQ495_001832"
     CDS             complement(282..398)
                     /locus_tag="VQ495_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2531753.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(613..966)
                     /locus_tag="VQ495_001833"
     CDS             complement(613..966)
                     /locus_tag="VQ495_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2531754.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            1219..3852
                     /gene="polA"
                     /locus_tag="VQ495_001834"
     CDS             1219..3852
                     /gene="polA"
                     /locus_tag="VQ495_001834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2531755.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3937..4374
                     /locus_tag="VQ495_001835"
     CDS             3937..4374
                     /locus_tag="VQ495_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2531756.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         1189 a          814 c         1068 g         1332 t
ORIGIN      
        1 taggctccat aatatccata ggggatttac ccactatgga tattatggag ccattttaca
       61 acagtgcctc aaattcagat actgacatac ctaattgctg gctggcaacc tcagaagtca
      121 gaagaccttg gcgtactatg tctaggaagg caaaaagttt cctttctgcc cttccttcaa
      181 ctttcccacg ctctagtcct tgttcaaggc cttcagccct agcagtttcc aaggcatagt
      241 cctgtgctaa caagacttgt tcttctcgca tatgtagttg actaaacatt ttcctgtcct
      301 cctcggacca gctcttgtag tccagcagtt gatttgcttg ggtaatggct cgttgcggtt
      361 gctgggtaaa gggcttgttg ccgaaaaact ccaaccatgg cttgcgaacc ttgtctttgc
      421 tggtttctct gtatttttta attccaagaa tgccatctta accagatggt tttcctgtcc
      481 attgttggta atcgccaata cctcacctgt tgtgtcttcg cgcatactaa agctatgaaa
      541 agccaggtca tctgagaaat aattactatc cacgatagca atgacgtaaa caggagcgat
      601 gtgtttgtag atctagtgag tatcaccttc tcgctgacga attttttcaa gattttgatt
      661 aacctgactg cacaggtaag cccacaagtg attgatgaaa aaattctgat gatggacttg
      721 aatctcaata attacttgag taccgttatc caactccgcc aagacgtcta tactggtata
      781 aaaatcctgc gctgagtagg gcatggaagg caagacatga atgttacttc cctccaaaat
      841 agtcacattt ttggctggca agtccagcat atcgcggata aattgacaag tgatttctgg
      901 gttgctgaaa attttcttag caaccaagtc attggtcggg ctaattccca gatgtctgag
      961 aatcatcctt ttcctccttc tatagtgaat tgaaataaga tgtgaacaac tctatcagga
     1021 aagtcaaatt aatttataga aatattttag cagtcaaggt gtactattat agattcaata
     1081 tactatatta tagcacattt ttaaatctag gtttactaaa ttctctggct ctatctattt
     1141 attcggaaaa agtttgaaaa atacttgctc tagctcttcc caatggtatt ttttgattct
     1201 ttcctttata atgggtgtat ggataagaaa aaattattat tgattgatgg gtcttctgta
     1261 gcttttcggg cgttttttgc gctgtatcag cagttggacc gttttaagaa tgcggctggt
     1321 ttgcatacca atgcgattta tggttttcag ttgatgttga gtcatttgtt ggagcgggtt
     1381 gagccgagtc atattttggt ggcttttgat gcgggaaaga cgaccttccg gacagagatg
     1441 tatgcggact ataagggtgg tcgggccaag actcctgatg agtttcgtga gcaatttcct
     1501 ttcattcgtg agttgctgga tcatatgggg attcgtcact atgagctggc tcagtatgag
     1561 gcggatgaca tcattgggac gttggataag ctagcagagc aggatggttt tgatattacc
     1621 attgtcagtg gggacaagga tttgattcag ctgacggatg agcatacggt ggttgaaatt
     1681 tccaagaaag gtgtggctga gtttgaggcc tttacgccag attacctcat ggaagaaatg
     1741 ggcctcacac cagctcagtt tatcgatctc aaggcgctca tgggtgataa gtcggataat
     1801 atccctgggg tgaccaaagt cggtgaaaag acgggtatta agctcttgct ggagcatggt
     1861 tcgcttgagg ggatttatga aaatattgat ggaatgaaga cttctaagat gaaggaaaat
     1921 ctcatcaatg acaaggaaca ggcctttttg tctaaaacac tagcgaccat tgataccaag
     1981 gcaccgattg cgattggttt agaggacttg gtctatagtg gtccagatgt tgaaaatctt
     2041 gggaaatttt acgatgagat gggattcaaa cagctcaagc aggctttaaa tgtgtcgtca
     2101 gctgatgtgg ctgagggttt ggattttact attgttgacc aaatcagtca agatatgctg
     2161 agtgaagagt ctatcttcca ctttgagctt tttggtgaga attatcatac ggataatttg
     2221 gttggatttg cctggtcttg tggggataag ctctatgcca cagacaagct tgagctgttg
     2281 caagacccga ttttcaagga tttcttagaa aaaacatctc tgagagttta tgactttaag
     2341 aaggttaaag ttcttttgca acgttttggt gtggatttgc aggcgcctgc ttttgacatc
     2401 cgtttggcta aatacctcct ttcgactgtg gaggacaatg aaattgcgac catcgctagt
     2461 ctttatggtc agacttactt ggttgatgat gaaactttct acggtaaggg tgttaaaaag
     2521 gccattcctg aacgtgagaa attcttggaa cacttagctt gtaaacttgc tgttttggta
     2581 gaaacagagc ctattttact tgaaaaactc agcgaaaatg ggcaattaga gcttctttat
     2641 gatatggagc aacctctggc ttttgttctt gccaagatgg aaattgctgg gattgtggtc
     2701 aagaaagaga ccttgcttga gatgcaggct gaaaatgagc ttgtcattga aaaactgact
     2761 caagagattt acgagctggc tggtgaggag tttaatgtca actcgcctaa gcagttgggc
     2821 gtgcttctct ttgagaaatt gggacttcct ctagaataca ctaagaaaac caagacaggt
     2881 tattcgacag cagtggatgt gttggagcgt ctcgctccta ttgctccgat tgttaagaaa
     2941 atcctggatt accgtcaaat tgctaagatt caatctactt atgtaattgg cttgcaggac
     3001 tggattttgg ctgatggaaa gattcatact cgctatatgc aggatttgac ccagaccggg
     3061 cgtttgtcta gtgtggatcc aaacttgcaa aatattcctg cccgattgga acaagggcgc
     3121 ttgattcgga aggcttttgt gccagagtgg gaggatagtg tgctactcag ctctgactat
     3181 tcacagattg aattgcgcgt tttggcgcat atttctaagg atgagcactt gattaaggcc
     3241 ttccaagagg gggcagatat ccatacttcg acagccatgc gggtctttgg cattgagcgt
     3301 cctgataatg tgactgcaaa tgatcgtcgc aatgccaagg cagttaactt tggagtggtt
     3361 tatgggattt cagactttgg cttgtctaat aatttgggaa ttagtcgtaa ggaagccaaa
     3421 gcctacattg atacctactt tgaacgtttt ccaggtatta aaaactacat ggatgaagtg
     3481 gtgcgggagg cgcgtgataa gggctatgta gagaccctct ttaagcgtcg ccgtgagttg
     3541 ccagatatca attcgcgcaa cttcaatatt cgtggttttg cggagcgaac tgctatcaac
     3601 tcacctatcc agggttcggc agcagatatt ctcaagattg ccatgattca gctggataaa
     3661 gccttggttg caggtggtta tcagactaag atgctgttac aagtgcacga tgaaatcgtc
     3721 cttgaagtgc ctaaatctga attggtagag atgaaaaaat tggtgaaaca aaccatggaa
     3781 gaagccattc aactcagtgt tcctcttatc gcagatgaga atgaaggggc aacctggtac
     3841 gaggctaaat aaaaaggggg ctagtcctcc ttttttgtag tagaattctg caaacctttt
     3901 caaaatgtgc tatactgatg aaaaaggagg ttttctatga gtcaagaatt tatcaatcca
     3961 agtgatggcg tgattcgtca gtatctcgca acgagtaaaa cccttgctgt ggtgggtttg
     4021 tctgaccgtg aagaaacaac cagcaatcga gtgacaaagg aaatgcaggc tcggggctat
     4081 aaaatcatcc cagtcaatcc caaggcggca ggtggcgaaa tcttgggtga aaaggcttat
     4141 gctagcctcg ctgaaattcc ttttcctgta gatattgtca atgtttatcg tcgcagtgaa
     4201 tttctgcccg atgtggcgcg tgattttctc aaagctgatg ccaagatttt ttgggcacag
     4261 ctaggacttg aaagtttaga agcgaaagag attttgcgtg atggtggatg tgatgatatc
     4321 gtgatgaatc gctgtatcaa gagagaacat actcgcttga ttgaggaagc ataagaaaaa
     4381 ggtagctgat gggctacctt ttg
//
LOCUS       DAVBCG010000137         4393 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBCG010000137 DAVBCG010000000
VERSION     DAVBCG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.137"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(26..1036)
                     /locus_tag="VQ495_001836"
     CDS             complement(26..1036)
                     /locus_tag="VQ495_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2531757.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1185..2354
                     /locus_tag="VQ495_001837"
     CDS             1185..2354
                     /locus_tag="VQ495_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2531758.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2351..3121
                     /gene="recO"
                     /locus_tag="VQ495_001838"
     CDS             2351..3121
                     /gene="recO"
                     /locus_tag="VQ495_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2531759.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3118..4110
                     /gene="plsX"
                     /locus_tag="VQ495_001839"
     CDS             3118..4110
                     /gene="plsX"
                     /locus_tag="VQ495_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2531760.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4116..4349
                     /locus_tag="VQ495_001840"
     CDS             4116..4349
                     /locus_tag="VQ495_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2531761.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
BASE COUNT         1276 a          836 c         1127 g         1154 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtgttaga aaatgccgat aagggtctgc ataccaagag
       61 tggtgaggat gatggcaatc cagcagatgg ctccaagaag aatggatttt ccactggatt
      121 tgaccatagc gaccagattg gttttgagac cgatagcact catggccatg acaataagga
      181 atttagagag ttctttgaga ggggtaaaga aactactgga cacacctaga gaggtgagta
      241 gtgtagtgag gagagaggca aggataaagt aaaggataaa aagtgggaag acttttttca
      301 gttgtaggct ttgcttgttt tcttgttggc gactttgcca gtaggagaga aagagcgtga
      361 tagggataat agccaaagta cgtgtgagtt taacaatggt tgcagactcg agggtattgc
      421 tttggtaaag actgtcccaa gcgctggcgg cagccgttac agaggaagtg tcgttgaccg
      481 cagtccctgc aaagagggcg aagccttcat tggatagatg aagccaggtg ccgagggttg
      541 gaaagatgag cgcagccaag acattgaaga aaaagataac ggaaatggct tgggctactt
      601 ccttttcctt agcatcaata acgggcgctg tcgctgcaat ggcagaaccc ccacagatag
      661 aagaacctac tccaaccaag gtagccagtt ttgtatccag tgcaaagaaa cgctggaaga
      721 ggtaggcaat aatcagagct attgagatag tggacaggat gacagggagt gaagattggc
      781 caactgcgaa gacctgcgag atattgagac caaaaccaag caagacaacg gcatattgga
      841 gcaacttctt ggaactaaag gtcaaaccag catccagttg tttataggac gagagaaagg
      901 gatgtaggag catgcctatg aaaatggcaa aaacgggcgc gccaatcaca gggaagaatc
      961 ctcctaagta ccaagatacg atagaaatga gaaggcaggc caagatacct gctccatttt
     1021 ttgatagaaa tgacataaaa acctccaaat aaaatctatt accattatag acctataaac
     1081 aggaaaagta aaatagaaag tggaaagcta ttctctaatg tatttttgcg gtcggggggc
     1141 ttttgtagta taatagagat acgttttgaa agtaggaggt atctatggac ttaactaagc
     1201 gctttaataa acagttagat aaaattcaag tttcgttgat tcgtcagttt gaccaggtta
     1261 tttcggagat tcctggggtc ttgcgtttga ccttggggga acctgatttt acaacgccag
     1321 accatgtcaa ggaggcggcc aagcgagcga ttgatcagaa ccaatcctac tatacaggga
     1381 tgagtggtct gctgactcta cgtcaggcag ccagtgactt tgttaaggaa aagtaccaac
     1441 tggactatgc tcctgaaaat gaaattttgg ttacaattgg ggcgacagag gctttatctg
     1501 cgactttgac ggctattttg gaagagggag acaaggtact tttgccagct cctgcttatc
     1561 caggctatga accgattgtt aacttagttg gggcagaaat tgttgagatt gatacgactg
     1621 aaaatggttt tgtcttgact cctgagatgt tggagaaggc cattttggag cagggtgata
     1681 agctcaaggc ggttattctc aactatccag ccaatccgac aggaattacc tacagtcgag
     1741 agcagttaga ggccttggca gctgttttac gcaagtacga aatttttgtt gtctgtgatg
     1801 aggtttactc agaattgacc tacacaggcg aagcccatgt gtctctagga acgatgttga
     1861 gagaccaggc tattattatc aatggtttgt ctaaatcgca tgccatgaca ggttggcgtt
     1921 tggggctgat tttcgctcct gcggccttca cagcccagtt aatcaagagt caccaatact
     1981 tggtcactgc cgcaaatacc atggcgcaac atgctgcggt agaagccttg acggctggta
     2041 aaaatgatgc ggagcccatg aagaaggaat atatccaacg tcgggactat atcatcgaaa
     2101 aaatgactgc tcttggtttt gagattatca aaccagacgg tgccttctat atttttgcta
     2161 aaattccagc gggctacaat caagactcct ttgcttttct gaaggatttt gctcagaaga
     2221 aggccgttgc ctttatccct ggtgcagcct ttggacgtta cggggaaggc tacgtccgcc
     2281 tatcttatgc agccagcatg gagactatca aagaagccat gaaacgactt gaggagtaca
     2341 tgagagaagc atgattcagt ctatcacgag tcaaggcttg gtgctttaca atcgcaattt
     2401 tcgtgaggat gacaagctcg tcaaaatttt tacagagcag gttggcaaac gcatgttttt
     2461 tgtcaaacac gctggtcagt ctaagctggc gcctgttatt cagcccttgg tgctggcacg
     2521 atttctcttg cgaatcaatg atgacggact cagttacatc gaagactatc atgaggtcat
     2581 gacttttccc aagattaata gtgacctctt tgtcatggcc tatgcgacct atgtggcagc
     2641 tcttgcagat gctagtttgc aggacaatca gcaggatgct cccttgtttg cttttttgca
     2701 aaagactttg gagttgatgg aagcaggctt ggattatcag gttttgacca atatttttga
     2761 aattcaaatt ttgactcgat ttggaatcag cctcaatttt aatgagtgtg tcttctgcca
     2821 tcgggttggt caggcttttg acttttcttt caaatatgga gcctgcctct gtccagagca
     2881 ttatcatgag gataagagac gttgtcatct caatcccaat atcccctatc tgctcaatca
     2941 atttcaagct attgattttg agactttgga gaccatttcg ctcaagcctg gaatcaagca
     3001 agagctacgc caatttatgg atcaattata tgaagagtac gttgggattc acctaaaatc
     3061 aaagaaattt attgattccc tagcagactg gggacaatta ctaaaagagg aaaagaaatg
     3121 aaaaaaatcg cagtagatgc catggggggc gattacgcac ctcaggccat tgttgagggt
     3181 gtcaatcaag ccctatctga cttttcagat atcgaggttc aactttacgg agatgaagct
     3241 aaaatcaagc aatatctgac agcgacagag cgcgtcagca ttatccatac ggatgagaag
     3301 attgattcgg atgatgaacc tacgagagct attaggaata agaaaaatgc cagtatggta
     3361 ttggcagcca aggctgtcaa agatggtgaa gcagacgctg tcctttcggc tgggaataca
     3421 ggtgccttgt tggcagcagg attcttcatc gtgggtcgta tcaagaatat cgaccgtcct
     3481 ggactcatgt ctaccttgcc taccgttgat ggaaaaggtt ttgacatgct agaccttggt
     3541 gccaatgcag aaaatacagc ccagcacctc catcaatatg cggttctagg ttccttctat
     3601 gctaaaaatg tccgtggcat tgcgcaacca cgcgttggtt tgctcaacaa cggaacagag
     3661 agtagcaagg gcgacccgct tcgtaaggaa acttatgaat tactggcggc tgatgaaagt
     3721 ttgaacttta tcggaaacgt ggaagcgcgt gatttgatga atggcgttgc agatgttgtt
     3781 gtggcagatg gtttcacggg aaacgctgtg ctcaaatcca tcgaagggac agctatggga
     3841 atcatgggct tgctcaagac agctattaca ggtggtggtc ttcgagcgaa actaggtgcc
     3901 ctccttctca aggacagcct cagaggtttg aaaaaacagc tcaattattc agatgttggt
     3961 ggagcggtct tgtttggtgt taaggcacct gttgtcaaga ctcatggctc aagcgatgcc
     4021 aaggctgttt atagtacgat tcgtcagatc cgtaccatgc tagaaacaga cgtggttgcc
     4081 cagactgcgc gtgaattttc aggagaataa aagagatgac agaaaaagaa atttttgacc
     4141 gtattgtgac cattatccaa gagcgacagg gagaggactt tgtcgtgaca gaatccttga
     4201 gtctgaaaga cgatttggat gcggattctg ttgacttgat ggagtttatc ttgactctgg
     4261 aagatgaatt tagtatcgaa atcagcgatg aggaaattga ccaactccaa agtgtaggag
     4321 atgtggttaa aatcattcaa ggaaaatagc aatcggagtt ccaagtcaac ggaagtagat
     4381 ggtttttaga aat
//
LOCUS       DAVBCG010000138         4304 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBCG010000138 DAVBCG010000000
VERSION     DAVBCG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.138"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..617)
                     /locus_tag="VQ495_001841"
     CDS             complement(<1..617)
                     /locus_tag="VQ495_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2531762.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLEN"
     gene            complement(726..1244)
                     /locus_tag="VQ495_001842"
     CDS             complement(726..1244)
                     /locus_tag="VQ495_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2531763.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VQ495_001843"
     CDS             complement(1471..2340)
                     /gene="rsmI"
                     /locus_tag="VQ495_001843"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2531764.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VQ495_001844"
     CDS             complement(2343..2660)
                     /gene="yabA"
                     /locus_tag="VQ495_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2531765.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2700..3590)
                     /locus_tag="VQ495_001845"
     CDS             complement(2700..3590)
                     /locus_tag="VQ495_001845"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2531766.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3587..4225)
                     /locus_tag="VQ495_001846"
     CDS             complement(3587..4225)
                     /locus_tag="VQ495_001846"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2531767.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
BASE COUNT         1194 a          979 c          685 g         1446 t
ORIGIN      
        1 taattctcaa gctgtgagtt cattatgatt tgtaaatccg atatatcttc agcagctaaa
       61 aacacatttt gaaatgttcc aagtccgttt tcaccaaaac tatattctct cttactatac
      121 ttactcttat tattctctat atagttagag tcaaggttct taacctcttg aagttcattt
      181 tctttacttc tgaggttaaa atcttttact tctgaagtta agttttttga cttctgtaag
      241 tcattttctt ttacctgaaa tatactcata aagtctttaa cataaatgat gttcggctta
      301 ccaagtccaa gccttactct ttcgatcagt cctattcctt tttttacatc aagctcatct
      361 aatgttttta tggcagttgg ctttgagata tttcttcttt tcataatttc ttcgacagta
      421 aaatagataa atactctgcc ttccttgtct atccagttat tcttaaatga cattcctgtc
      481 tgtttaagaa gtatggaata aaggataatt gcttctgctg ataagccccg aaattcttct
      541 ctatcaacta atatctccgg tacttttaag aagttaaatc tttccgcttc tctgttataa
      601 aaatattcaa agtccatgca agatcacctc ccttcttaaa aatttgcaaa gaaaaagacg
      661 atagtttttc ttctatcgcc tttggtaacc atttttatga aattttttaa agttaaaaat
      721 ataatttata atactatctt cttccatttt ctgattttag ttctgaaagt tccaaaaggt
      781 gcaaccgtat ttacatgaat aaacttatac acttcccaag tcgctgtttt agtcgcttca
      841 tcagcccact ttctcatatg cggttcaaat aattcttcct cactcaacga atcaatcatt
      901 gcagagatag aattaatatt ttcatttaat tttgctttca actcttgcag agataaatga
      961 gcgtaggtat ctgtgaacca ctgatataat tcaccaagtt gattccattt aaatttatcc
     1021 gatggtgttt ttacttgaag cccctttctt tcatcttctt cccatttaag aaccaagttg
     1081 gtccaaccaa cctgataagc aaggttttct gctggagttc tgtcaacttc atcagctctc
     1141 ttatctttta aattttctgg aatattatca aattctaaaa tatatttctc aaatgttttc
     1201 tctatctcag cttttagttc ttctttattt tcgtatgttc gcaaaaaatc accttctttc
     1261 tgatttatca atcctttgat ttaaaatgac cttatttttc tatttatacc ttctaagaac
     1321 gctataactg ctgctccaaa catcggtatt ccgtatgggc ttaataaaaa acttaatacc
     1381 aaagcttcta taccaagtga tacatcttta tattatcatc actaattaag ttaaacagaa
     1441 acagacagaa ttattacatc ctgtctccct ttattgtttt tcttcccaat cgtggtaggc
     1501 agcgtagagt tgactcttat tccactggta aatcttagct acttccttaa tagcttgatt
     1561 tttcttcatg ccttgctgga tacgggcttg gatttctaag aacaagtcct cctcgtcttt
     1621 ttcctccaca tcctgactgg caccttcaac aataagaagg cattcgccct tgagtggcgt
     1681 ttcagcgatg ctttccagta attcagaaat tgtacctcgt tggtattctt catagatttt
     1741 ggtcaattcc ctgaccaaaa caaccgagcg gtcaccgtag acttctaaca tattttccaa
     1801 cgtgtctgct acacgatgag gtgattcata aaaaatctgt gtttcaggat aatctttttt
     1861 agagccaaaa aattgcttct gttgacctga ttttctcggt aaaaaaccgt aaaagatatg
     1921 tggctgtggc gctaaaccac tggcaatcaa ggcagaaatt cctgcagagg tacctggaac
     1981 agtcacaact gcaatttctt cctcaatagc tgccttaact aaatcatgac cagggtctga
     2041 aatgctaggc aaaccggcat cagagacctg agcaatactt tgccctgctt tcaagaaacc
     2101 aatcaaatca ggaatttttt ccttggcatt gtgctcatga aaactgatct gcttggtgga
     2161 aatgtcaaaa tgcttgagca aaagccctgt attgcgcgta tcctcagcag caatccagtc
     2221 cacttctttc aaggtctgga tagcacgaaa agtcatatca tctagattgc caatcggcgt
     2281 tgccactaga tacagcttgc catagggaga ctgcccctta aaactttttt gaatttgcat
     2341 gcttactccc tgtataacaa ctcatcacag aacatacatt cttcgtcctg ctctcgacgt
     2401 tgtccataaa aatcattaca tacgtgaaat ccatcacggt aaatacgacg gacactttcg
     2461 cgaacatgct tggccttgac gggagcgtct gcttccacct cacccaagcg ttctcgcaac
     2521 ttactatttt ccaagcgaag agctgtattt tcttctacca agctcttgag atttttcttg
     2581 atggcttcca catcggctaa agttaccaat aattgttggg aaaaatcatc cagcgcgtca
     2641 aataattctt ttttgtccat aaaacagccc tttcctttct ttatcattca tttttgagtt
     2701 tatatttctt tcaagaccag atattccatg gcattttgaa agctgacatt agcttgccac
     2761 atttttctag cttctagtaa atcttgtaga atcactctta ctcttacctg caagaggtcc
     2821 tgcccacaga gaacttcaag aatccgtaaa acctgatcct gtttttcctt atcatctgcc
     2881 aagttggcta atttggcaac ctgtagataa ctttcttttt tcttagctac taaccaagtc
     2941 agcaggcgtt cactttcatc gaccaaggtc caaaaacttg cctgattagc caacttttct
     3001 gcttcagctc gcgattgact aaacttagct aaaagagtcg cttttttctt aacaagtccc
     3061 atttgttcta agagtaagat aagtttttct tcttgctttt taaagtggaa gatctgagtc
     3121 cgacttcgga ttgtcggtaa catcttttcc tcatcgctag tcaagaagaa aatataaact
     3181 tcactctggg gttcttcgat gaccttgagc agagaattgg ctgcgttggg atgcatttta
     3241 tccgcttgct cgatgataaa gacctgttgc tggctttcaa tccctgcttg agaaaactgt
     3301 cccaccaatt cccgaatacg ttctgtcttg atgacctgat tgactggctt aatcaaggtg
     3361 acatctggaa actcttcctg ttcaatcagc ttgcaacttc ggcatttctc acatggtaag
     3421 acgccaactt tatccgtaca aaagaggctc ttagctaaaa attgcgccat ttccaagctt
     3481 ccaaagaaac ctgaaaagag ataggcgtga ttgagctggt cttgttctaa gatacggaca
     3541 aaacggtcaa actgagctgg ttgccaagcc tttagttgat cttgtttcat ttggccaagc
     3601 ccattccgtc aaacaagaca gccttggtag tttccacaac ttgctccaaa gggagactag
     3661 catcaatctt gacaatgcga tttccctctt tatccagaag agaaaggtag ccttgacgaa
     3721 cttttttatg caagtccaac ccttccaaat ccaaacgatt aacctcgcgg tcactattag
     3781 cagcaatacg agccagccct tcttccacct cgatgtcaaa atagagtgtc aaatcgggtt
     3841 tgaggccatc tgtcgcaaac tgattgagcc agtcaatggc ttcaatatct aagccacgac
     3901 caaatccctg ataggcaaca gaactatcga taaaacgatc catgatgacc aacttgccag
     3961 cttcaagggc tggaagaact ttttccacca aatgctgtct gcgactggca atatagagaa
     4021 gtagctctgt tttagcatcc atctgagtat gacttggatc caaaatcact tcccgaatct
     4081 tctccccaat caagactccg ccaggttcac gggtcgtcag cacctttact cctttttcct
     4141 ctaaaattgg tagcagagcc tctaaaacac tggtcttgcc tgctccctct ggtccctcaa
     4201 gagagactaa aaatcctttt gacatgtcta actcatttct tttttactac ttctattcta
     4261 tcaaaaaaat aggggtttgt gacaatcttt ttgtcttctc attt
//
LOCUS       DAVBCG010000139         4274 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVBCG010000139 DAVBCG010000000
VERSION     DAVBCG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.139"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..131
                     /locus_tag="VQ495_001847"
     CDS             <1..131
                     /locus_tag="VQ495_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531768.1"
                     /translation="PPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSCFN"
     gene            complement(124..297)
                     /locus_tag="VQ495_001848"
     CDS             complement(124..297)
                     /locus_tag="VQ495_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2531769.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(455..2062)
                     /locus_tag="VQ495_001849"
     CDS             complement(455..2062)
                     /locus_tag="VQ495_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2531770.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2215..3699
                     /locus_tag="VQ495_001850"
     CDS             2215..3699
                     /locus_tag="VQ495_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2531771.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3794..>4274
                     /locus_tag="VQ495_001851"
     CDS             3794..>4274
                     /locus_tag="VQ495_001851"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2531772.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1239 a          982 c          811 g         1242 t
ORIGIN      
        1 ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat atcaaaaagc
       61 acctcaaaaa ggtattacca agttgcaata ccttttatga ggctcttttg tcttgttctt
      121 gtttcaattg actatattat cagtgtaata gtctaataga tagttaaatg tagttggatt
      181 tccactaaag gttgatgagt aagtttttgt atctgaacct gattggccgc aagcagctaa
      241 aagcaaaaca gatgcaaaag tcaggcctgc actaaggaca cgcttcttta tgttcatctt
      301 ctttctcctt aatagtggga atttgtaaag ttaattgaat ttcgagaatg aaagtttata
      361 aaccttggtt ataaaaaaca aaggatttct gcttttatac agtcctccct tgtttttata
      421 tgatttcaat tttaaatttt tctgcaaaaa atatttatag taattccaca cagaaagcat
      481 cccatggaac taagatttgt ttttcaaaga cttcttgagc tagggtgttt tcaatcaaga
      541 cagatttgac ttttccttct actgtcaagt cttgctcttc attggacaag ttagccacaa
      601 ctaggaagcg acggtcgcca tccttacgta tataagcaaa gaccttatca gccgtatcaa
      661 gcaattcaaa gtcagctcga actagccagc tattctcctt gcgaatttgg accagtttct
      721 gataggtata gaaaatagaa tctggatttg ccagcgcttc ttggacattg atcatctcgt
      781 aatttggatt aaccgccaac caaggttgac ctgttgagaa accagcgttt ttgctctcgt
      841 cccattgcat aggggtacgg gcattgtcac gtccaataac acggatactg tccatgattt
      901 cttcaatcgg aacacctttt tcaagagcct cacgcgcata gttgagagat tcaatatctt
      961 ctacttgatc cagtgtttca aacggatagt tggtcatccc aatctcctca ccttggtaga
     1021 tataaggagt tcctctcata agatgaagca agattgcaaa ggctttggca gatttttcgc
     1081 ggtattcttg gtcatttccc cagattgaga caatacgagg gaggtcatgg ttgttccaga
     1141 agagggaatt ccagccgtcc tcaactccta actctgtctg ccatttgttg aagatttctt
     1201 ttaacttagc gatattcagc tctttttgat agtgccattt aggctgacct tcctgatact
     1261 gaagaccgat atgttcaaac tggaagacca tagacaattc ttgccccttt ggatcagagt
     1321 agagcttggc aatctctggc gttgctcccc aagtctcccc tactgtcaag agatccttat
     1381 ctccaaaggt cgcctgattc atttccttga gatagggatg gagcatagga ccattattga
     1441 ctaccttctc gtcaggaatt ttgccaatca tgtcaataac atccatacgg aaaccaccaa
     1501 tacccttatc aatccagaag ttcatcatct cataaatttt ctggcgaagt ttttcatttt
     1561 cccagttgag atccggctgt ttcttgctga aaaagtggag atagtattga cctgactttt
     1621 catcgtattc ccaagcagac ccactaaaga tagaatctaa atcgttaggc tcatcccgcc
     1681 agatatagta gtctcgctca gggctattag gattttcaca ggcctctaca aaccaggcat
     1741 gctcatccga ggtatgattg accaccaagt ccatgatgat acggatatca cgcttcttag
     1801 cttctgcaat cagctgatcc atgtcctcca tggttccaaa aatagccgca atcgcttgat
     1861 aatcagcaat atcgtagcca ttatcatcca tagggctgtc ataaacggga gaaagccaaa
     1921 ttgctgtgat tcctagcttg gctagatagt ccaacttact ggtaattcct ggcaaatcgc
     1981 caattccatc tccattacta tccataaaac tctttggata gacttgatag actacggcat
     2041 tatgccacca tttttcttgc atctgtttac ctcattattt tcataatcta tagtctatga
     2101 tagcactttt taaaattttg tgcaagcgtt tgcttaatct ttttaatttc ttttttagct
     2161 ccgtagtttc ctaacttcca attttttatt ataatagagt ccagaggtaa aaaaatgaaa
     2221 aaacaagctt ttagttctga acagtatttg aatttacaac gcgaccatat tttggagcgc
     2281 attaaccaat ttgacggcaa gctctactta gagtttggcg gtaaaatgtt agaagatttc
     2341 cacgctgctc gtgtccttcc tggttatgaa cctgacaaca aaatcaagct cttgcaagaa
     2401 ttgaaagagc aggttgaggt tgtgattgcc attaatgcta gcaacattga acattccaaa
     2461 gcacgtggcg acttgggcat ttcttatgac caagaagttc ttcgtttgat tgacaaattc
     2521 aatgaattag ggatttttgt tggttccgtt gtcattacac aatacgctgg ccaacccgct
     2581 gcagatgcct tcctcaacca acttgagaaa aacggaattg attcttatct tcattatcca
     2641 atcaaaggat atccgacgga tatggatcac atcatttccc cagaaggcat gggcaaaaac
     2701 gactacatca aaaccagtcg caacttgatt gtcgtaaccg ctcctggacc cggttctgga
     2761 aaattggcaa cgtgtatgtc caatatgtac cacgaccaaa tcaatggcat caagtctggc
     2821 tacgctaaat ttgaaacctt ccctgtttgg aatcttcccc ttcatcatcc agttaacttg
     2881 gcttatgaag ctgccacagc tgaccttgat gatgtcaaca tgattgaccc cttccatctt
     2941 caaacctatg gagaaaccac tgtcaactac aaccgtgata tcgaaatctt cccagtgctc
     3001 aagcgcatgt tggaacgtat tctcggaaaa tcaccatacg cttcaccgac agatatgggt
     3061 gtcaacatgg ttggtttcgc tattacagat gacgaggctg ctgtcgaagc ttctaaacaa
     3121 gaaatcatcc gccgttacta tcaaacagtt cttgacttca aagctgaaaa agttggcgaa
     3181 gctgccgtca agaaaattga gttgctcatg aacgacctcg gtatctcacc tgcagaccgt
     3241 aaggttgctg tcgttgcgcg ccaaaaagca gaagaaactg gcggaccagc cctagctctt
     3301 gaattaccaa atggtgacat tgtcactggt aaaaactcag aactctttgg ccctacagcc
     3361 gctgccttga tcaacgccat caaaaaatca gctgacatcg ctaaagaagt aaaactaatc
     3421 gagcctgaag ttgttaagcc aatccaagga cttaaaatcg atcatctcgg tagccgcaat
     3481 ccacgccttc attcaaatga aatcctgatt gcacttgcta tcacagctac agaaaatcct
     3541 gatgctgccc gcgctatgga agaactcggc aacctcaaag gaagcgaagc ccactcaacc
     3601 atcatcttga ctgatgaaga caagaatgtc cttcgtaaac tgggtatcaa cgtaaccttt
     3661 gacccatact accaatacga ccgcttatat cgtaagtaaa gatttcaacc tctccagcct
     3721 cggagaggtt ttctctctgt ctcaggagca agctttatga tataatgaga gaagaaaact
     3781 gaaaggattt accatgtcaa aagaagttat tgtcgaaagt tttgaacttg accacaccat
     3841 tgtcaaagca ccctatgttc gcttgattgg ggaagaaaca ggaccaaaag gagacatcat
     3901 ctccaattat gatattcgct tggtgcaacc aaacgaagac tctatcccta ctgccggcct
     3961 tcacactatc gagcacctct tagccaaact catccgtacc cgcattgacg gcatgattga
     4021 ctgttcacca tttggttgcc gcacaggctt ccacatgatt atgtggggac gtcacaccag
     4081 tgctaaaatc gcagctgtta tcaaggattc gctcaaggaa atcgctgaaa ctactacttg
     4141 ggaagatgtc ccagggacaa ccatcgaatc ttgcggaaac tacaaggacc acagcctctt
     4201 ctctgctaaa gaatgggcaa aactcatctt ggaacaaggg atttcagatg atgcctttga
     4261 acgtcatgtg attt
//
LOCUS       DAVBCG010000140         4242 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVBCG010000140 DAVBCG010000000
VERSION     DAVBCG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.140"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(60..659)
                     /locus_tag="VQ495_001852"
     CDS             complement(60..659)
                     /locus_tag="VQ495_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2531773.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(660..1757)
                     /locus_tag="VQ495_001853"
     CDS             complement(660..1757)
                     /locus_tag="VQ495_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2531774.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(1757..2494)
                     /locus_tag="VQ495_001854"
     CDS             complement(1757..2494)
                     /locus_tag="VQ495_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531775.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(2496..3380)
                     /locus_tag="VQ495_001855"
     CDS             complement(2496..3380)
                     /locus_tag="VQ495_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531776.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(3367..3561)
                     /locus_tag="VQ495_001856"
     CDS             complement(3367..3561)
                     /locus_tag="VQ495_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531777.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(3558..3764)
                     /locus_tag="VQ495_001857"
     CDS             complement(3558..3764)
                     /locus_tag="VQ495_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531778.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
BASE COUNT         1343 a          942 c          725 g         1232 t
ORIGIN      
        1 tattgttcca ttcactaccg tcaattttag cacatagtct tcatgaaaat attatatcat
       61 cataaccaac cagattcttt tgcgatatta gctgcctctg ttcgattacc agcatccagt
      121 tttgaaagaa tattggtgac atagtttcga atagttccgt ttgagaggta aagctgatct
      181 gcgatttctt ggttggataa gccccgggcg attcccttta agacagcaat ctcttgttct
      241 gtcaacggat tgggacgggt catcaccact tccatcaatt caggcgaata ctccttgcgt
      301 ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa tgcttcgttc ctttaataca
      361 taagcatcca ctccagcctt gaccgcacgt tcaaaatacc cagcacgctt gaaggtcgtc
      421 accacaacca cctttgtttc aagcctttct gatcgtatcc actccaagac ttcaagacct
      481 gtcttaacag gcatttctac gtcaaggatg gcgatatcta cagactcctt ttctaatagt
      541 tggattgctt cttgcccatt cttggcttga aggacagact ccacatctgg ttgaagcgtg
      601 agcaattgac acatagcatc tcgcaacata ctttgatctt ctgcgactaa tactttcatc
      661 tactttctct ccttataagg tagtcgaact tgcacttccg ttggatgttt ctgactgatt
      721 acacttactt ctcctgaaaa tggaaggaca cgttctcgaa ctgtatggag ctcattttct
      781 gttacagttt caaaaccttt cccgtcatct ctgaccatca ggagcaattc ctgatctgtg
      841 cgttctaatt ttaagtaggc tttagacgct ttagcatgtt tgatgatgtt ggtcactaat
      901 tcaagcaaaa tcatggaagc cgttgactct aattcctgag ttaagctagc tgtatctagt
      961 tgattagcta tttccacctc aattccagca atttctaaca tctttttcac agtctctagt
     1021 tcggatgtca aagttctaga cttaagattt tccacaatgg ttcgcacttc acgcattgat
     1081 tctttgctaa tctgctgtat ttctcttaat tccttttcca cttgtggata agcctgtatt
     1141 tgaaataact gcaaggctag atcagtttta acactaagca tagcaaaggt atgccccaga
     1201 ctatcatgca aatcttgacc gatacgattg cgctcatttt ctgctagcaa tagatttatc
     1261 tgggtatttt gcttagtctg agcttctttc aaatcctcga caaggcgaat tcgaaccgag
     1321 cccaaagtca tcaaatcaac aaaagtaaga attccaagca gatagactag aaactcaact
     1381 tcgattctct gaaaaatcaa aagtcgcccc acaacaagga cttgagcaag aagaaaagtc
     1441 cagacatgta aagactttaa actacgtacg ctgaaatgat aacttaagag attggaaagg
     1501 aaaaagaaaa accagatata attcacagca acaaaggcgg tattcccagc tacatagatc
     1561 agcatgatcc cccagaaaag ccaagatagg cgctggctct tagttgttaa aacacccaaa
     1621 tatgccacta caaatagaat atcaatcaat aaatgccagg cagaaagcca cccagtcact
     1681 acaggtagga tggggaaaat cataaaaatt aaactggccc aaaacatata gtgtatgctt
     1741 ttcagtcttt caagcattaa gcattctcct tatgaccttg aaggtaaatg gtcaaaccaa
     1801 acaaaactgc tgaaaaaaca agtagataaa ctgtggctga tagattgatg ctaccctcgt
     1861 ttaagaaggt cttgagcaac tccatcaact gataagttgg taggcacttc ccgattgctt
     1921 gcatccagtc tggaaataaa gagatgggca tccagagtcc acctaaaaca gccaagccta
     1981 gatagagaag attgcccacg acagacatca gctgactaga aggcaagagt gtcaaggtca
     2041 agccaagcgc tacaaaagct atacttccta ctatcagcaa gaacgcagcc ccaatccagc
     2101 ttcctaaaga catatccaca cctctgacca agtgcccaac tgagaaaaca accaggattg
     2161 agaccaaata atcaaccatc atacttgtta tctttgatag ataatattct accatattta
     2221 cagggctatg acgcaatgtt ttctgccagt tgttcatctt atcggtatgt aagacagctg
     2281 ggaatgaaaa gatagcggtc gacatcatgg aaaaggcagt catggagatg aggtagtcgc
     2341 gcataaaatt agccggtcca cctggtgtgt cctggtacat gcctgaaaag aatagataga
     2401 aggctgtcgg catccctaca gataataaat aatagactga ttgccgtttg gtcaatagaa
     2461 attctatctt gtttagtgca atccatcgtt tcatcttagt catctccctt ctgtgtttct
     2521 tcaaagattg tatccaacaa actacgatta ttgacttcga tttcttgtat cctacatcct
     2581 gcttgaacta acagttccca gaaagcatcc gcttcgcatg tgactacttg taaggcatct
     2641 tgtttttgta cccagttttc aaccaagact gacttctcaa cgacttcctt gtaagctagc
     2701 ggaaggataa aatgcttttc aatctcctca ctacgcatag ctagaggcgt cgtgtcacga
     2761 atcaactctc ccttatttaa aaccaaaatc cggtcagctg tatgctctac ctcttcaata
     2821 taatgagacg aatagagaat ggtgactcct tgcgctttta ggtcccgaac aatttcccaa
     2881 aaacgttgac gagttgaagt atccatggca gcagttggct catctaaaaa gacaagcttt
     2941 ggtcgcccaa ttaaggtcaa gataaaagat aaaagacgct tttgcccacc tgacaatttt
     3001 tctgcgaatt gctctttctg ttgctggtca aactgtaata gttgatcgat ttcctggttg
     3061 ttcaagggat ttggatagat acgttgaaag aaagcaatca actctttaac ctttagtttt
     3121 tgaacaatta cattttcttg agggagatag gatctcgtat agtctaactt agagctcgtt
     3181 accggcaaat cttggatata tacttgccca ctggtgacca atttatctcc aagcaaacag
     3241 tccaagagtg tcgtcttacc agctccattt ggcccaatca aggcgacgca ttcaccttca
     3301 gctacctcaa aggaaatatc cttcaaaata gccttacttt tgatgttttt atgtaagcct
     3361 tgtaccttaa tcatgttcat aatattctcc tttcaaccac tccattccca tagggaaaac
     3421 gacggaaatc ataaatccta atccccaggc gccgcgaata acttggtgaa gaaaggattg
     3481 atcaaaccaa cctgtaaaca tttctaccaa ccataccact agtgatagtc caataaaaag
     3541 aaagagaatc gcttttttca tttttcaagc tcctttttca catctcttac taatttcaag
     3601 ccttctcgta tcaaccaaga catgattcca aagcttgcaa acaactccca aggaaaatga
     3661 tagaatcctt cgtccaatcc tgagaacatg agataagtca tgactcctgc tactactaaa
     3721 ctcactgcga taatcatttt atttctcatc tcttcttcct ccatttcata ctacaattat
     3781 agtcttttga aatcagagga gatagaagct tctgtcacta aaaaatatga caaatgtcat
     3841 aaaaaattct gttcaaaaca agcaagatac actatacaat aaaacacaat tagataaatc
     3901 taaggcaact tcctcaaaag agatatcaaa ccccattcac actataatgt aaactaatac
     3961 ttatttaaag tcaaaagagc ggaaattttt atcgtacaaa catatatatt ataggtaaga
     4021 caaaaaactc ttcaattact ttcttaaaaa tttataggag ttataaaatc tattaaattt
     4081 caatgtggat acctaaacta cagtatttat tgaaaagact ggagacaaaa agtatacgct
     4141 gccaaaatga attgctgaaa atcaaaaaag agagaagcaa actgattctc tcttaatgta
     4201 tataatatct agttataaaa atacacactc acatatctct gt
//
LOCUS       DAVBCG010000141         4190 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVBCG010000141 DAVBCG010000000
VERSION     DAVBCG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.141"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..1275)
                     /locus_tag="VQ495_001858"
     CDS             complement(<1..1275)
                     /locus_tag="VQ495_001858"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2531779.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQTLRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLG"
     gene            complement(1353..2021)
                     /locus_tag="VQ495_001859"
     CDS             complement(1353..2021)
                     /locus_tag="VQ495_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2531780.1"
                     /translation="MSLSMSSADGENLKNEKLSIKDQFLTIYRNLRLVFLKSGQKNFG
                     FMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALFINQFCVLVAVIISSLTGND
                     YFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFFTLYVSGKVQPKISAMLMKN
                     LAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLTWMLFVGLSLLAIFLTILNL
                     KNDI"
     gene            complement(2333..2599)
                     /locus_tag="VQ495_001860"
     CDS             complement(2333..2599)
                     /locus_tag="VQ495_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531781.1"
                     /translation="MKLLFRNQAYRLLTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFF"
     gene            complement(2789..3640)
                     /gene="rgg"
                     /locus_tag="VQ495_001861"
     CDS             complement(2789..3640)
                     /gene="rgg"
                     /locus_tag="VQ495_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET2531782.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELLV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSYNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNFFHTILLNGFLASIECEKFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            3764..4105
                     /locus_tag="VQ495_001862"
     CDS             3764..4105
                     /locus_tag="VQ495_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2531783.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIEDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         1328 a          883 c          819 g         1160 t
ORIGIN      
        1 tcctagtttt tcaaagattt ctgagaagtt ttggctgatt gtctcaagtg acacttgcac
       61 ttcttctcgg gtttggttgt tcttgaccgt cacttgtccg ctttcgactt cgctctctcc
      121 tagggtgatg agggtcttag ccgcaaagac atctgctgac ttgaactgag ctttgagctt
      181 acggttgagg taatcacgct ctgctttgaa accttgttgg cgaagagttt ggactagttc
      241 tagggctttg acatttgctc catcacccaa gactgcgata tagacatcta gggcgttttc
      301 gatagggagg gccacgcctt gtttttcaag gatgagaagc aggcgctcta caccaagccc
      361 aaaaccaaat ccagcagttt cagggcctcc aaagtaagca accaaaccat cgtagcgacc
      421 acccgcacag actgtcaagt cattgccctc aatctctgtg ataaactcga aaatggtgtg
      481 gttgtagtag tccagaccac gcaccatatt ggtatcgatg atgtaatcta ctccaagatt
      541 ttccaacatc tgacgcacag catcaaaatg agcttggctt tcttcatcaa gaaagtccaa
      601 gatagacggc gcattctcta ctgccacctt gtcttctttt tccttagagt ccaagacacg
      661 aagaggattt tcctccaagc gacgttggct atccttagac aaggtctcct tgagcggtgt
      721 caaatagtca atcaaggctt ggcggtaggc tgcacggctc tcaggatttc caagagtgtt
      781 gaggtgcaat ttgacacctt gaataccgat ttccttcaaa aaatgggctg ccataacgat
      841 tgtttccaca tcggtagctg gattgctaga gccaaaacac tcaacaccaa tctggtggaa
      901 ttggcgcaag cgccctgcct gtggacgctc ataacggaac ataggtccca tgtagtagaa
      961 cttgcttggc ttttgcactt ctggggcgaa gagtttattt tccacatagg aacggacaac
     1021 gggcgcagtt ccttctggac ggagggtaat atgacggtca cccttgtcat aaaaatcgta
     1081 catttccttg gttacgatat ccgttgtatc tccgacagag cgactgataa cctcgtaatg
     1141 ctcaaaaata ggcgtgcgca cttctgcgta gttgtagcgt ttgaaaattt cacgggcaaa
     1201 gccctcaacg tactgccact tggcagattc agcaggtaaa atatcctgcg ttccttttgg
     1261 tttttgtaat ttcataggga atcctcttta aacttaatag tcttatttta ccataaatag
     1321 agggattaaa acagtgagaa aaaaattagg atttagatat catttttgag attaagaatt
     1381 gttaaaaaaa tagctagcaa ggaaagacca acaaatagca tccaagtcaa ctgcatgttc
     1441 catacggcta ctagtgaaaa acaagctgtt cccacaggta tggataaggt aaacaataga
     1501 cctaaaaaat tactagtacg agctagaacc tctggagcta gatttttcat gagcatggca
     1561 ctaatctttg gttgaacttt accagacaca tacagagtaa agaagagaaa tagcaaacca
     1621 agcacgactt gattgaataa attagccaaa ccaactagac taagtcctac ggtcgcccac
     1681 atcatcaatc taggcaagga ctgcttccca aaataatcat tgcccgtaag gctactgatg
     1741 atgactgcta ctaaaacaca gaattgattg ataaatagtg cctctgtata agaaaaattc
     1801 aagagagaat ggctcaaaaa gaagatatta taaattccac ccaaagcgcc acccaaggaa
     1861 ttaataagca agacagcaaa gagcataaaa ccaaagtttt tctgtccact tttaagaaaa
     1921 acgagacgta aatttcggta aattgttagg aactggtctt tgatagaaag cttctcattt
     1981 tttaagtttt caccatcagc agatgacatt gacaggctca atttgctttt tcctaaaaag
     2041 aggatagtgg ctgatacgag gaaaaagcaa gcattaattc ccgcaacgag ggaaaaattg
     2101 ttgaccgata gagctaagag ccagactccg aaagcttgac caccaatagc tgaaatatag
     2161 gtgatgaact gtgaaaaaga ataagcctcc atcagatcat cttcagctac tttttcctta
     2221 ataagaggca tacgcaggcc acctgcaaaa tcactgatga tatcactaat gacattgatc
     2281 aaacacaggc tagaaaaggc aaagagacta gcttgctgaa caactagggc tgctagaaaa
     2341 atagaaccgc ctgaaacaaa ccgctataga ccatccattt gaccttgtcc ctcgtgtaat
     2401 ctgcccgaat ccctgcaaaa actgtaaaga aggtcggaag aatcatgaca atattcgcca
     2461 tagcaacagc aaaagatgct tgtgacaagg tcgatgcata gacgataaag accaggttga
     2521 aaatcgaagc accaaaagca ttgaagaagc gtgacaaagt caataggcga taagcttgat
     2581 ttctaaacaa tagtttcata gataatctcc ctccttgttc taagttctac atcaagaata
     2641 gctttagtat agcacttatg aatttgcgaa acgattcttt tgaatatatg caacaaataa
     2701 aatgagcaaa aaacgataga acttttgtaa tgacctgaga aagggcaaca gttctatcgc
     2761 ttcaaatttt ttcttagttt gcagatattc aacattcggt cgtatttcta tagtattcgg
     2821 cagatttatt acagccaagc atctcaaaaa tacggacagc atcctccatc tttttctggc
     2881 cttccttgac tctaccttgc ttgctatcaa ggagaccttc tgcccacaga taaacaattc
     2941 ggaaataggt ctcattttcc ttgtagaaat gctcttcgat aacacgttta aaataatagg
     3001 cattggtaaa tttttcacac tcaatactag ctaaaaagcc attcaatagt atagtatgaa
     3061 aaaagtttcg attaccagac atttccatta gaaaatcaga tttacgtacc atttctcgta
     3121 catatctagt aaaaagagaa acagataaaa atggagaact gactgaaaat aaattgagtt
     3181 catagattcc ccagatctcg gtagaaaaca aataatcatg aaggactttt ccttcctctg
     3241 ctgttaactc taccctttca tctatgctct tcatataaga cttgataata atggcattta
     3301 gaatatgttt ctgtttgttg taagaatggg catgcttttg atactccctg cgatataagt
     3361 cctcaagagg tgctatattc tttggttcca agacatctgt aatttctttt ctcaactcag
     3421 aatctgtatc atactggaaa cctctcgcca gaaagaggat ctcctccaca ctggcagata
     3481 tattttccag agcaaataga aacttttcca ccaaaagctc actctgacct gtttcaaaac
     3541 gggacaacat agacggcgaa aattgtcctc cggttgcttg tctcagtgag atatttcttg
     3601 actctcgtaa ttgtctaaag acttttccaa tctgctccat agacttcccc ttgattccgt
     3661 attttcttca ttttatcata tttttcagaa aattcatcaa aaacttgcca aattgtcaga
     3721 attatgagaa aatagaggat atttatcacg tggagggact gctatgagag acgatatcaa
     3781 aatcaatgac cgtgctttgg ccttgcaaga ccaaattatc gaaaaactag agaaagtttt
     3841 tgatacagat gtggaattgg atgtttacaa tctaggtctg atttatgaaa tcaatctgga
     3901 tgaaacgggg ctctgcaaga ttgtcatgac cttcaccgat actgcctgtg attgcgccga
     3961 aagcctgcct attgaaatcg tggcaggtct gaaacaaatc gagggtatcg aagatatcaa
     4021 ggttgaagtt acctggtcgc ctgcttggaa aatcacacga atcagtcgct atggccgtat
     4081 tgcccttgga ctaccacctc gttaagcagg ccaatcactt ttaaagatga aaaaagcaag
     4141 ccgaaaatgg cttgcttttt gatactcaat gaaaatcaaa gggcaaacta
//
LOCUS       DAVBCG010000142         4180 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVBCG010000142 DAVBCG010000000
VERSION     DAVBCG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.142"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            127..1293
                     /locus_tag="VQ495_001863"
     CDS             127..1293
                     /locus_tag="VQ495_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2531784.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            1409..2446
                     /locus_tag="VQ495_001864"
     CDS             1409..2446
                     /locus_tag="VQ495_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2531785.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            2654..3508
                     /locus_tag="VQ495_001865"
     CDS             2654..3508
                     /locus_tag="VQ495_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2531786.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            3658..3915
                     /locus_tag="VQ495_001866"
     CDS             3658..3915
                     /locus_tag="VQ495_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2531787.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         1249 a          749 c          956 g         1226 t
ORIGIN      
        1 agattttagt tggaactcga ttcaattcat gtgacaacgt gaaaatcgtt agagcatttt
       61 atatagaata tacatgggaa tgtagcttac tcccattccc atatttaata gaaaaagagg
      121 aactcaatgc tacattatac aaaagaagac ttgctcgaat tgggtgcaga aatcactacg
      181 cgtgaaatct accaacagcc tgatgtatgg agagaagctt ttgaatttta tcaagcaaaa
      241 cgtgaagaaa ttgcagcctt cctacaagaa atcgctgata aacatgacta tattaaggtt
      301 atcttgacag gtgctgggac ttctgcttat gtgggagata ccttgctacc ttattttaag
      361 gaagtctatg acgaacgcaa atggaatttc aatgctattg cgacaacaga tatcgttgcc
      421 aatccagcaa cctatttgaa aaaagatgtg gcaactgtcc ttgtgtcttt tgctcgtagt
      481 gggaattcgc ctgaaagttt ggcgactgtt gatttggcca aatccttggt ggatgagctt
      541 tatcaagtga cgattacttg tgcagcagat ggtaaattgg ctcttcaagc tcacggtgat
      601 gatcgtaatc tcttgctctt gcaaccagct gtctctaatg atgctggatt tgccatgact
      661 tctagcttta cgtctatgat gttgacaact ctcttggtct ttgatcctac agaatttgct
      721 gttaagtctg aacgttttga agttgtatct agtcttgccc gtaaagtttt agacaaggca
      781 gaagatgtca aagagctcgt tgatttagac tttaaccgtg tcatctatct aggcgctggt
      841 cctttctttg gacttgctca tgaagctcag ctcaagattt tggaattaac tgctggtcaa
      901 gttgcgacca tgtatgaaag tccagttggc ttccgtcacg gtccaaaatc tcttatcaac
      961 gacaatacag ttgttttggt ctttggtaca acgacagact acactcgtaa gtacgacttg
     1021 gacttggttc gtgaagttgc tggtgaccag attgctcgtc gtgttgtgct tttgagtgat
     1081 caagcttttg gtcttgaaaa tgtcaaagaa gtggcccttg gttgtggcgg tgtcttgaat
     1141 gatatttacc gtgtcttccc ttacatcgtt tatgcccaac tctttgcttt attgacttca
     1201 ctcaaggtag aaaataaacc agatacaccg tctcctacag gtacagtaaa ccgtgtagta
     1261 caaggtgtca taattcacga atatcaaaag taagacagtg tttatgaatt cttgacaaga
     1321 ggatttgtaa attatcagat aaaccataga ttgtcagtac gctttctatg gtttgtttgc
     1381 ttgagagaaa tagtaaaagg agaacagaat gaaagcatac acagagcgtg tatttggaaa
     1441 tgttgagggt gaggatgtct tggcctatcg atttgagaca gacggtggct accaacttga
     1501 ggttatgact tatggtgcga ctatcttgcg ctatgtcgca cctgacaagg ctggaaattt
     1561 tgccaatgtt atcttgggat ttgatgactt tgatagttat gtaggcaata gtcccaagca
     1621 tggagcaagt gtaggtcctg tagcgggtcg tattgcaggt gcgacctttg agctcaatgg
     1681 taagacctat gaccttgagg ttaataatgc tagcaactgt aatcacagtg gttcaactgg
     1741 ttgggattcc agcttgtttg aagttgaaga agtaagcgat catggcttga ctctctacac
     1801 agagcgtaca gatgggacag gagggttccc tggaaatctc aagatttgga tcagttatca
     1861 cttggaagaa actggtgcct atgaaatcag ctacaaggta acgaccgatc aggatacgct
     1921 ggtcaatcca accaaccaca gctatttcaa cttgtctggt gatttcacgc agacgattga
     1981 ccgtcatgtc ttccaactaa acacagaggg catttactca atcgctcctg acggtgttcc
     2041 tgccaaaact ccagaagcca accgtgatgt ggtcaaacac gtctacaatg gtaccttgtt
     2101 gaaggatatc tttgcagaag aagatgagca aatccagctg gcatcaggtt tggatcatcc
     2161 atttgccctt cctgcaggcc atgacaatgc tggattcctt tatgaccaaa attcaggtcg
     2221 cttcctgctt ttcaagacag aagctccttg ctttgtggtc tacacagcaa actttgtgga
     2281 tgaaagtgtc atcataggag gtcagccaat gctacagcac aatgggattg ctcttgaagc
     2341 gcaagcttta ccagatgcca ttcacagtga ccttaaaggc caagtcattc ttaaagctgg
     2401 tcaaaccttc accagtaaga cacgttatga acttgttgtg aaataaaaaa agctggtcaa
     2461 aaagtcttta aaattaaaat aacgcatatt gtcaggtgat gaaaagtttg ataatatgcg
     2521 ttttattatg aaatgattta ctttatatta tcctgaaatg gtgttatttt tgagttatct
     2581 ggcttgactt tcttgatgga aattatataa tagttgtaac aatgataatt acaactagaa
     2641 aagaggacca tttatgacct atgaatacaa gagtcacatt tatttggcag agacagcttt
     2701 aaatgtaaag gatttggcca gtcaaacagc cttttatcag caagttattg gtttagaaat
     2761 cttatctcaa actgagacag agtccattct aggccttggt ggaaaagtct tggtacagct
     2821 gattcaagca caagagagcg gagaagtgag ggaatattat ggtctttacc atttggccat
     2881 tctcttgccc acacgcaagg ctttagctga tgtcttgaaa cacctgacag atttacaaat
     2941 tcctcttgtc ggcggtgcag accatggtta cagtgaagct atttacttgg aagatttgga
     3001 gggaaatggc attgaactct atcgagataa gccagtttcc acatgggata ttcgagaaga
     3061 tggacgcatt atcggagtga cagaggctct tgcggcgcag gacatttatg agttggggga
     3121 aagagtagag ccctttatcc tggcagaagg tacgagaatg gggcatattc atctttccgt
     3181 caaggatagt cgaaagtcca gccagtttta tcaaaaggtg ttagggctag aggataaatt
     3241 cagtgtgcct agcgctagtt ggattgcggc tggagattac catcatcatt tagcagttaa
     3301 cgaatgggga ggaaaaggtc tggcttcgcg taagcaaggc ttaccaggtt tagcctacta
     3361 tgtcatcgaa gtcgcacaca aaaaagaact attaacgatt gcccaacgag cacaagaagt
     3421 tgacgcacca atcaaatgga tgacatcgag ccaattggaa atcacagact cagatggcat
     3481 cgtgacccgt attcgtttag caaggtaaaa ttaggaattt ttaataagtt ataagtattg
     3541 ctagtttgct ttttgaacgg tctttggtaa cagaactgcg tgagctggat gggcgagaat
     3601 tttgacaaag aagctgatta gagatataat gaagaaaaat cgagcaagga aaaagaaatg
     3661 acaaattcaa agtatattac ttgtttgaaa cgttcagagg gccagttgtg tgggattcaa
     3721 aagatgattg aaggagatcg tgactgtgct gatattgtga cgcaactgac agcagtgaga
     3781 tctagtgtag agcgcgtgat tgagatgata attaccgaaa atcttactga atgtatcaat
     3841 cagccactag atgattctga agctcaaaag gaacgcttag aaaaggctat ccgatacttg
     3901 attaaacgga aataaatgaa tgttttgaga cgatatgtgc aaaggtctat taactattag
     3961 aatagtttaa tgggagtcaa aagaagttgt aaaaaatctt taaaattaaa aaaacgcata
     4021 gtatcaggta ttgaaatgta ctgaccccaa aaagttagat tttttctgtc taacttttgg
     4081 ggtgcagttc ataagaacct tggtaatatg cgttttatta tggggagatt tatacttttc
     4141 gaaaatctct tcaaaccagg tcagcttcac cttgccgtag
//
LOCUS       DAVBCG010000143         4138 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVBCG010000143 DAVBCG010000000
VERSION     DAVBCG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.143"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            40..681
                     /locus_tag="VQ495_001867"
     CDS             40..681
                     /locus_tag="VQ495_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2531788.1"
                     /translation="MRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAGA
                     FSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNEEFKTSAQLLT
                     NKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRI
                     PATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(723..2711)
                     /locus_tag="VQ495_001868"
     CDS             complement(723..2711)
                     /locus_tag="VQ495_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531789.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2713..3471)
                     /locus_tag="VQ495_001869"
     CDS             complement(2713..3471)
                     /locus_tag="VQ495_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531790.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3899..4090
                     /locus_tag="VQ495_001870"
     CDS             3899..4090
                     /locus_tag="VQ495_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531791.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1257 a          819 c          803 g         1259 t
ORIGIN      
        1 cacgaaattg attcaaattt tgcaggtcgt ttaaatatcc tgcgtgcggg tgttcttgat
       61 gctaacgatg gaattatttc cattgctggt gtggttatcg gggttgccag tgccacgacc
      121 aatatctgga ttatcttttt atcaggattt gcggctatct tagctggtgc cttttcaatg
      181 gctggtggag aatatgtatc cgtttcaact ccaaaagata ccgaggaagc tgccgtttcg
      241 cgagaaaaac tcttgctaga ccaagatagg gaactagcca aaaaatccct ctatgctgct
      301 tatatccaaa atgaagaatt caaaacttct gcccaactct tgactaataa gatctttctt
      361 aaaaatccac tcaaggctct ggtagaggaa aaatatggga ttgagtatga agaatttacc
      421 aatccttggc acgctgccat ttctagcttc gttgcctttt tccttagaag tttgcctcca
      481 atgctgtcag tgaccatttt tccaagtgat taccgcatcc ctgctaccgt ccttattgtc
      541 ggtgtggccc ttcttctcac tggttacaca agtgctagac ttggaaaagc cccaaccaaa
      601 acagctatga ttcggaacct tgctattggt ctcttgacca tgggagttac cttcctgctc
      661 gaacaacttt tcagcattta gaatacaaga aatacctcga ttttgaagtc gaggtatctt
      721 ttttacattt gcacaatctt gcgataactt cttgaagtaa tcatgaaaat cagcacatag
      781 gcgatgagga agatagcgca gatagacaag gtcacaatca acatcatagt cgtatccagt
      841 acaccaatca cttttaaaat cagactaagc atatggtagg caaaggctag atgtaggaag
      901 gcaaagagca aaggaaggaa gaatacagtt aaaacctgtt tgttgatggt ttgcttgatt
      961 tgcttttgat ctaaaccgac tttctgcaag ataataaagc gctcacggtc ttcatatcct
     1021 tcagaaattt gtttgtagta gatgaccaga acggttccga ccataaagat aatggataag
     1081 aaaataccga taaagaagac accaccaaag agggcactca tctgagcact agcatctgct
     1141 agagtgctac catacacata gttaccttca gtgtttaatt gagcattaaa cttttgtaag
     1201 tatttttcat actcctcagc gaccttgagt tgttctgctt cactggcatt tacattcata
     1261 ccaccgtaaa actgattata gatagccgaa tctgggaatt gatccaaaaa ggcttgtaaa
     1321 tcaggtacaa caaggtaatt gtaatcagca gtcaaaatat taaactgatt tggaacatgg
     1381 ttgacaataa aatctttagt aaattcttct tttacagaaa attgatgatc atttagagtt
     1441 agagctttct gttctttaac tccctcattc tttgcaaaga gtccgacctc atttcctgat
     1501 agagacagtt tttgaccagt catattttca taatcttttt ggtcaaatac catgaaaact
     1561 gtttttggtt ggacacggtt ttgtcctttt tcaaaaatag ttaacttagt tccttcttga
     1621 tttgcaatac caaagttact gtaacgaagt acttctttct ctttgacact ataacctttg
     1681 tcacttgcaa actggctcaa gagtttgtcc aagtcttctt tttcaacatt ttgccctgaa
     1741 accccaaaat catgaggatt tagaactttt ttaaagcttt ctgaggaatt gaaaatgctt
     1801 gtcgctgctg acatggttac caaaaccatt gttgacaaga tagcgatggt ggctagtcca
     1861 actgcatttt tcttcatacg gaaaatcaag ttagaaacag atatgagatt atttggttgg
     1921 taatagtatt tcttattttt ctttaagatt tggaggaaaa cggtaatccc tgcattaaac
     1981 aagagataag tcccaaagat aaccagtaaa acagctatga agaaggttgt taaggctgta
     2041 agaggatctt ttaccgtaag ggcaagataa tagccaattc ctaaacttat ggaaccaaga
     2101 atggtttgga gaggaaggaa gcgacctttt ttctctccac tagctttctc acgagagagc
     2161 tggagggcat tcatacgggc gattcgaagg gcattcagga acatgaggcc taggaaaatc
     2221 aaaccgaaga caacaagcac tgtaatgaca actttcgtct ggaaggtagc aaccagctca
     2281 accttcaatt tcattagttt gagcaggaaa gcgaaaatta acttgtcaaa caaggctcca
     2341 ataccgatac ccgctccaac agttagaatc ccaaatacca ctaactcctt aaaggtcata
     2401 ctgataagat gacgcttctc caagcccaac atgccataaa ttcctagttc cttggaacgg
     2461 ttcttcatga caaaactatt ggcatagaga acgataatgg ctgacgcaag ggtgacgaca
     2521 aacataccaa atccaagtgt agcctgaatg gttgttcctc cacgaatttc agcaatcttg
     2581 ggattgaagg ttagagagta aaagagatag gtgacagtga ctgccaagag aacagccagc
     2641 gcaaaaggat agtagagttt gcggttttta atcaagttcg ataccgctaa cttattggtt
     2701 aatcgaaaca tactaattca cctcgcttgc catgacagtc aaggtatcag agatttcttg
     2761 gaacatctga cgttctgtct tctctccacg gtagatttga ttgtaaagaa tgccgtcttt
     2821 gataaagagt acacgtttag ctctgctagc tgctgctgtt gagtgggtta ccatgagaat
     2881 ggtttggccg cgctcattga tttcatcaaa gacatcaagt aaggctgcag atgacttgga
     2941 gtcaagagct cctgtcggtt cgtccgcaag gagaatttca ggttcagtga tgatggcgcg
     3001 tgctactgct acacgctgtt tctggccacc agaaatctcg taagggtact tctcttgtaa
     3061 ttggtttatt cccagattct cagctgtcac caccaatttc ttcatcatct ctgtaatagg
     3121 tcttcttgac aagacaagcg gaagcaagat attgtccttt acagatagag tatctagcaa
     3181 gttaaagtct tggaagacaa agcccaactt ttcacgacgg aagctagagg cctgtgaatt
     3241 tttaatggtt gcggtgtcag ttccattcaa gtaaacctga ccacgacttg gtttatccag
     3301 catagctaga atattgagaa gagttgattt accagaacca gactcaccca tgatggcaac
     3361 gtagtcaccc ttttcgacgg taaagtgaat atccttgagg gcttctactt ggttgccctg
     3421 aaaacgtgtt ttataaattt tttgaacgtg ttttacatct aaaagtgtca tgagaatctc
     3481 ctttcttaat atcccatcaa atgaaatgta gcttgcatca tagtgcatcc cagctgaacg
     3541 cgctcaattt ctttgtgact tggttttcta gttttcttat gtaagatttg tttcatgatg
     3601 ttactccttt gttctttatg agtctagttt acatcaaaaa aagaccgctt gccataacct
     3661 aaccttacaa aagagacttt aatcttacat ttttgtaaga ttgggtgaaa tataagcaaa
     3721 attacttaga aaatgatttg atttcctatt tttaaagttg taaaatataa gtatgaaaag
     3781 gcttactttg gaaaggagga agcgggaact atcatttgag taagcttacc tagataacat
     3841 accattcaat ttaacacttg caagtcgttc aacctataag gaaagaaggt gcgctttaat
     3901 gaaacacaaa gaacatatac tcatcggact tctctatctg ctctctccat tcatcggtca
     3961 gctcttagtt gaacacacac actttatcgg tacagaattt accggcactg cttatgttat
     4021 ttgctggtta tcagttgtta tcagcatcca ccatttttcc aaaaatgtat tgtctcagca
     4081 gcaaaaatag attaaaaaac tgagtttaaa ttactgcttt tataataaaa tgaaataa
//
LOCUS       DAVBCG010000144         4111 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVBCG010000144 DAVBCG010000000
VERSION     DAVBCG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.144"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            278..505
                     /locus_tag="VQ495_001871"
     CDS             278..505
                     /locus_tag="VQ495_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531792.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            793..3690
                     /gene="nanA"
                     /locus_tag="VQ495_001872"
     CDS             793..3690
                     /gene="nanA"
                     /locus_tag="VQ495_001872"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2531793.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGNRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1417 a          665 c          888 g         1141 t
ORIGIN      
        1 atgagtttct gttttctttt tatggactat aaatgttcag ctgaaactac tttcaaggac
       61 attattatat aaaagaattt tttgaaacta aaatctacta tattacacta tattgaaagc
      121 gttttaaaaa tgaggtataa taaatttact aacgcttata aaaagtgata gaatctatct
      181 ttatgtatat ttaaagatag attgctgtaa aaatagtagt agctatgcga aataacagat
      241 agagagaagg gattgaagct tagaaaaggg gaataatatg atatttaagg cattcaagac
      301 aaaaaagcag agaaaaagac aagttgaact acttttgaca gtttttttcg acagttttct
      361 gattgattta tttcttcact tatttgggat tgtccccttt aagctggata agattctgat
      421 tgtgagcttg attatatttc ccattatttc tacaagtatt tatgcttatg aaaagctatt
      481 tgaaaaagtg ttcgataagg attgagcagg aagtatggta taaatagcat aagctgatgt
      541 ccatcatttg cttataaaga gatattttag tttaattgca gcggtgtcct ggtagataaa
      601 ctagattggc aggagtctga ttggagaaag gagaggggaa aattggcacc aatttgagat
      661 agtttgttta gttcattttt gtcatttaaa tgaactgtag taaaagaaag ttaataaaag
      721 acaaactaag tgcattttct ggagtaaatg tcttatttca gaaatcggga tatagatata
      781 gagaggatca gtatgaatcg gagtgttcaa gaacgtaagt gtcgttatag cattaggaaa
      841 ctatcggtag gagcggtttc tatgattgta ggagcagtgg tatttggaac gtctcctgtt
      901 ttagctcaag aaggggcaag tgagcaacct ctggcaaatg aaactcaact ttcgggggag
      961 agctcaaccc taactgatac agaaaagagc cagccttctt cagagactga actttctggc
     1021 aataagcaag aacaagaaag gaaagataag caagaagaaa aaattccaag agattactat
     1081 gcacgagatt tggaaaatgt cgaaacagtg atagaaaaag aagatgttga aaccaatgct
     1141 tcaaatggtc agagagttga tttatcaagt gaactagata aactaaagaa acttgaaaac
     1201 gcaacagttc acatggagtt taagccagat gccaaggctc cagcattcta taatctcttt
     1261 tctgtgtcaa gtgctactaa aaaagatgag tacttcacta tggcagttta caataatact
     1321 gctactctag aggggcgtgg ttcggatggg aaacagtttt acaataatta caacgatgca
     1381 cccttaaaag ttaaaccagg tcagtggaat tctgtgactt tcacagttga aaaaccgaca
     1441 gcagaattgc caaatggtcg agttcgcctg tatgttaatg gtgttttatc tcgaacaagt
     1501 ctgaaatctg gcaatttcat taaagatatg ccagatgtaa cgcatgtgca aatcggagca
     1561 accaagcgtg ccaacaatac ggtttggggg tcaaatctac agattcggaa tctcactgtg
     1621 tataatcgtg ctttaacacc agaagaggta caaaaacgta gtcaactttt taaacgctca
     1681 gatttagaaa aaaaactacc tgaaggagcg gctttaacag agaaaacgga catattcgaa
     1741 agcgggcgta acggtaaacc aaataaagat ggaatcaaga gttatcgtat tccagcactt
     1801 ctcaagacag ataaaggaac tttgatcgca ggtgcagatg aacgccgtct ccattcgagt
     1861 gactggggtg atatcggtat ggtcatcaga cgtagtgaag ataatggtaa aacttggggt
     1921 aaccgagtaa ccattaccaa cttacgtgac aatccaaaag cttctgaccc atcgatcggt
     1981 tcaccagtga atatcgatat ggtgttggtt caagatcctg aaaccaaacg aatcttttct
     2041 atctatgaca tgttcccaga agggaaggga atctttggaa tgtcttcaca aaaagaagaa
     2101 gcctacaaaa aaatcgatgg aaaaacctat caaatcctct atcgtgaagg agaaaaggga
     2161 gcttatacca ttcgagaaaa tggtactgtc tatacaccag atggtaaggc gacagactat
     2221 cgcgttgttg tagatcctgt taaaccagcc tatagcgaca agggtgatct atacaagggt
     2281 aaccaattac taggcaatat ctacttcaca acaaacaaaa cttctccatt tagaattgcc
     2341 aaggatagct atctatggat gtcctacagt gatgacgacg ggaagacatg gtcagcgcct
     2401 caagatatta ctccgatggt caaagccgat tggatgaaat tcttgggtgt aggtcctgga
     2461 acaggaattg tacttcggaa tgggcctcac aagggacgga ttttgatacc ggtttatacg
     2521 actaataatg tatctcactt aaatggctcg caatcttctc gtgtcatcta ttcagatgat
     2581 catggaaaaa cttggcatgc tggagaagcg gtcaacgata accgtcaggt agacggtcaa
     2641 aagatccact cttctacgat gaacaataga cgtgcgcaaa atacagaatc aacggtggta
     2701 caactaaaca atggagatgt taaactcttt atgcgtggtt tgactggaga tcttcaggtt
     2761 gctacaagta aagacggagg agtgacttgg gagaaggata tcaaacgtta tccacaggtt
     2821 aaagatgtct atgttcaaat gtctgctatc catacgatgc ataatggaaa agaatacatt
     2881 atcctcagca atgcaggtgg accgaatcgt gaaaatggga tggtccactt ggcacgtgtc
     2941 gaagaaaatg gtgagttgac ttggctcaaa cacaatccaa ttcaaaaagg agagtttgcc
     3001 tataattcgc tccaagaatt aggaaatggg gagtatggca tcttgtatga acatactgaa
     3061 aaaggacaaa atgcctatac cctatcattt agaaaattta attgggaatt tttgagcaaa
     3121 gatctgattt ctcctaccga agcgaaagtg aagcgaacta gagagatggg caaaggagag
     3181 atgggcaaag gagttattgg cttggagttt gactcagaag tattggtcaa caaggctcca
     3241 acccttcaat tggcaaatgg taaaacagcg actttcctaa cccagtatga tagcaaaacc
     3301 ttgttgtttg cagtagataa ggaagatatc ggacaggaaa ttattggtat agctaaagga
     3361 agcatcgaaa gtatgcataa tcttcctgta aatctagcag gtgccagagt tcctggcgga
     3421 gtaaatggta gcaaagcagc ggtgcatgaa gttccagaat ttacaggggg agttaatggt
     3481 acagagccag ctgttcatga aatcgcagag tataagggat ctgattcgct tgtaactctt
     3541 actacaaaag aagattatac ttacaaagct cctcttgctc agcaggcact tcctgaaaca
     3601 ggaaacaagg agagtgacct cctagcttca ctaggactaa cagctttctt ccttggtctg
     3661 tttacgctag ggaaaaagag agaacaataa gagaagaatt ctaaacattt gattttgtaa
     3721 aaatagaagg agatagcagg ttttcaagcc tgctatcttt ttttgatgac attcaggctg
     3781 atacgaaatc ataagaggtc tgaaactact ttcagagtag tctgttctat aaaatatagt
     3841 agattgaaat aagatgtgaa caactctatc aggaaagtca aattaattta tagaaaaatt
     3901 ttagcagtca aggtgtactg ttatagattc aatatattat gtgactatta accttctctt
     3961 ctcctaaaat tgactttctt gttttcttat cttgtccact cgaaacaagt attgtaagaa
     4021 tttgattatt tttgaaagta cttttaatat acttgatata gttaaaaaag atttgaaact
     4081 aaattccaaa ttagaaaaag acttgaaata c
//
LOCUS       DAVBCG010000145         4046 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVBCG010000145 DAVBCG010000000
VERSION     DAVBCG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.145"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..370)
                     /locus_tag="VQ495_001873"
     CDS             complement(<1..370)
                     /locus_tag="VQ495_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531794.1"
                     /translation="MLLVLVSLVHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYKLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSG"
     gene            complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQ495_001874"
                     /pseudo
     CDS             complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQ495_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQ495_001875"
     CDS             complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQ495_001875"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2531795.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2422..3018)
                     /locus_tag="VQ495_001876"
     CDS             complement(2422..3018)
                     /locus_tag="VQ495_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2531796.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQ495_001877"
     CDS             complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQ495_001877"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2531797.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1136 a          927 c          734 g         1249 t
ORIGIN      
        1 aaccactccg acgctgatca tggtggtaca gcgtgattgc ttgattctca gctttcccag
       61 acaaaaaagt ccaatagtag gtcagatggc tatcactctc tagaacccga taagaggttt
      121 catccgcatg aagaagaggt tgttctaaca acttttctcg taaaagctta taaagggact
      181 ctaaatagta ttgacttgcc ttgatatgcc aattagagat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattggg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaaactg agccaaagct atgcaccaaa gagaccagca
      361 ccaacaacaa gataattgta catcttaagc tcctttggag ttcattatct gcctctttta
      421 ttagactaga aaatcattct actacttatc tcacttcttg tttgtaaaat tcttgcaatg
      481 catcttgcca agttggaata acaaatccag tagctttggc tttggccagg ctcatcgttg
      541 agtttagcgg acgttgagct ttggctggaa attgactgga atctactggc ttgacttcga
      601 catctgtatc tttcaaaatt tcaactgcaa aatcatacca tgttgtgtct tctgtcgcat
      661 catttgacaa atgataataa ccaaattcct tacgattttc agctaggtag atcatgaatt
      721 cagccaaggt acgagtccaa gtcggacgac cgtactggtc atttacaact gttaaagtct
      781 tatgagtttt cgcaagattt tgcatggtaa aaacgaagtt tttgccataa tttccaaata
      841 cccaggcagt acggataata tagaaattag acacatgctt ctcaactaac tcttccccca
      901 tacgcttagt gcgtccatat tctgtctgtg gatctggtcg gtcatcaact tcccactctt
      961 gtccaactgg tttcttaccg tcaaagacct agtccgtaga aatataaact agagttgcac
     1021 catgcttttc agatgctttt gcgacatttt ttgtccccat cacattgatg gcgaagtcca
     1081 actcttttcc ttcatcctct gctgcatcaa cagcggtgta ggctgcacaa tggtagacta
     1141 aagtcggttt cacctcttca aaaactttct caaccatttc ttcattggta atgtccatct
     1201 cagccacatc tactgccacg tattcttcat tacgttcatc caataaatag cgaagttccg
     1261 ttcctaattg gccatttgcc cctgtaatta aaatcatctc tagacctttc taatatgtaa
     1321 ctcttcccaa tatagcaata aaaagcagcg atttcctgct ttttatactg taataatctc
     1381 ctgagtctta gcataattgg cttcaacagc ttctttttct gctttccacc attcttggtt
     1441 atctgtatac cacttgattg ttgccttgag cccagcttca aagttggtaa attcaggttt
     1501 ccaccccaac tcatcacgga gcttgctggc atcaatcgca tagcgaaggt catgtcctgc
     1561 acggtcagtc acatgatcat aggcatccgt agcttgtccc atttccttaa ggataagttc
     1621 caaaacttcc ttattgttct tctcaccatc agccccaatc aagtaggttt caccgatttg
     1681 cccttttgtc aagattgtcc aaactcctga agaatggtca ttggtatgaa tccagtcacg
     1741 aacgttctta ccttcaccgt aaagttttgg cttaattcca cttaggatgt tagtaatctg
     1801 acgtgggatg aatttttcga tatgttgata aggaccgtag ttatttgaac agttggaaat
     1861 cgttgccttg actccaaaag aacgcaccca ggctttgaca atcaagtctg atgcggcctt
     1921 ggttgatgag tacggagagc ttgggttgta ttttgtttca gcagtaaatt tctcacctgg
     1981 tccttcacca tgacctggca aatcttctcg taaagggaga tccccataaa cttcatctgt
     2041 cgatacatgg tggaagcgaa tatcatactt acgagcagct tctaaaagag tatagattcc
     2101 aatgaagtta gtatgaataa atggcgatgg atcattgagc gaattatcat tgtggctttc
     2161 cgctgcataa tgaacgatag catctgcttg agcaaccaac ttgtctacca actccgcatc
     2221 agcaatgtca ccaacaacta actcaacacg attgcctaaa atttcctcaa tattcgcgcg
     2281 gtttccagca taagtcaact tatctaggac tgtcacgtga acatctggaa agttctcgta
     2341 aacataatgg acaaagttag aaccgataaa gccagctcca cctgtcacga taatattttt
     2401 gtattcagtc atattctttc cttattccaa atcttctttt ttcaaaggct tcacgtcctt
     2461 aagtagggga tgatgtttgt ctgcttcaga tacgtctgct tcttccagat tttcccattc
     2521 aataccaagg cttggatcag cgtagttcac aaaggcatac ttgggtttga gttcaagagc
     2581 ccagtaatca ttgaccagat agctatatga cactgtatct gataaaactt ggaagccatt
     2641 agctacgcct cgaggaacaa agattccctt gcttgcatca attactgtct gataggtatt
     2701 cccaaaggtt tcaccctcgc gtagatcaac ccaagaaccc agaactttcc ctccatctgc
     2761 tacagagatg tacttatccc aaggctctgc gtggaggcct cgaaggacat ttttacgtga
     2821 gaaggataca ttgttttgca attttccttc tgcaaagaaa gactctggaa atccaagtgg
     2881 aagcactttt tccttttgga aattttcttt aaaccagcca cgattatctc catgaacggg
     2941 gatatcaaac tccaacatgc ctggaatagc ttcaaccttg cgtgccgcaa gcgtcttacc
     3001 gaaaaaatta tctgtcatct atgcttctcc aatcaaacgg agcagatact gtccgtattc
     3061 atttttctta agtggttggg ctaaggccaa tacatcttct cgactgatat agcccatacg
     3121 gtaagcaatt tcttctaagt ttgctacctg aacattttgc atccgttgca ctgtttcgat
     3181 gtactgtgaa gcctctagta aactttcatg agttccagta ttcagccaag caaagccacg
     3241 tcccataagc tcaacagaca aatcaccacg atctaggtaa gccttgttta catctgtaat
     3301 ttccagttca ccacgagggc ttggtttaat actcttggca atttcgacaa catcgttatc
     3361 atagaaatac agtcctgtca ctgcatagtt ggagcgagga tactccggtt tttcttctat
     3421 ggaaatagca ttcatgtctg tatcaaactc aaccacacca aaacgctctg gatccttcac
     3481 ttggtagcca aaaacagtcg cacctttctc tttcttggct gctttttgaa gcattgtgct
     3541 cagaccagga ccatggtaga tattgtcgcc taaaatcaag gcaacgctat cgtcagaaat
     3601 aaactcttcc ccaattgcca aaccatctgt acttggttgc tctgcataag aaagtttgat
     3661 cccaaactca gatccgtctt gaagaagctc ttgaaatcga tgtaaatcct gtggagtgga
     3721 aataatcaaa atatccctaa tcccagccaa catcaatgtt gaaagtgggt agtaaatcat
     3781 cggtttatca taaaccggca taagttgttt tgacgcagcg cgagtcaaag gatataaacg
     3841 tgtccccgaa ccacctgcta gaataatacc tttcatagta gggtaccttt ctttgttatt
     3901 ttagagtcaa ttactattaa ttgtgtactg ctctaaaaag aaaacaatca tcccatgttt
     3961 tatatggctc tataatattt gtagtgggta aatccactat agatattatg gagcctattt
     4021 tattgtagaa aaaaagtccc ataaga
//
LOCUS       DAVBCG010000146         3889 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVBCG010000146 DAVBCG010000000
VERSION     DAVBCG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.146"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(112..1782)
                     /locus_tag="VQ495_001878"
     CDS             complement(112..1782)
                     /locus_tag="VQ495_001878"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2531798.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2012..2701
                     /gene="coaB"
                     /locus_tag="VQ495_001879"
     CDS             2012..2701
                     /gene="coaB"
                     /locus_tag="VQ495_001879"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2531799.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2713..3264
                     /gene="coaC"
                     /locus_tag="VQ495_001880"
     CDS             2713..3264
                     /gene="coaC"
                     /locus_tag="VQ495_001880"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2531800.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3248..3811
                     /locus_tag="VQ495_001881"
     CDS             3248..3811
                     /locus_tag="VQ495_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2531801.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         1163 a          873 c          699 g         1154 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaatc ttattgatga actgcttgca gtctgagaaa
       61 aaatgagctt ggatattatt tccaaactca cttaaagtca atttcaatcc actagaacaa
      121 gcctagtaca gttccatcgc tttcaacatc catgttgaga gctgctggac gttttggaag
      181 acctggcatg gtcataacat caccagttaa ggcaacgatg aagcctgcac ctaattttgg
      241 taccaattca cgaatggtaa tttcaaagtt ttctggtgct ccaagcgcat ttggattgtc
      301 tgagaaactg tattgagttt tagccataca gattggcaat ttgtcccaac cgttttgaac
      361 gatttgagca atttgtgttt gagctttctt ctcaaagttc actttgctac cacgatagat
      421 ttcagtgaca attttttcaa tcttttcttg gacagaaagg gcattatcgt acaaacgttt
      481 atagttagct ggattttcag caattgtctt aacaactgtt tcggcaagtg ctactccacc
      541 ttctgctcca tcagcccaga cactagccaa ttcaactggt acatcgattg aggcacagag
      601 ttcttttaag actgcaattt cagcttctgt atcagataca aattcgttaa tagctacaac
      661 tgctggaata ccgaacttac ggatattttc aacgtggcgt ttcaagttag caaaacctgc
      721 acgaactgcc tctacatttt cttcagtcag agcgtcttta gccacaccac cattcatctt
      781 aagggcacga agggttgcga caatgacaac tgcatctgga gatgttggca agtttggtgt
      841 cttgatatca aggaatttct cagcaccaag gtccgcacca aaaccagctt cagtaacagt
      901 gtaatcagcc aagtgaaggg ctgttgtcgt cgccaaaaca gagttacagc catgagcgat
      961 attggcaaat ggaccaccgt gtacaaaggc aggtgtaccg taaattgtct gaaccaagtt
     1021 tggcttaata gcatccttca aaatcaaagc caaggcaccc tcaacctgca aatcacctac
     1081 agaaacaggc gtacggtcat agcgataacc aataacgata ttcgccaaac gacgtttcaa
     1141 gtcctcgatg tccgttgcca agcaaagaat tgccatgatt tctgaagcaa ctgtaatatc
     1201 aaaaccatcc tcacgtggaa taccgtttag aggaccacca agaccaacag tcacatggcg
     1261 gagcgcacgg tcgttcaagt ccacaacgcg tttccagagg atacgacgtt gatcaatttc
     1321 cagctcattc ccttggtgca agtggttgtc aatcaaggca gaaagggcat tgttggcagt
     1381 tgtaatagca tgcatatctc cagtaaagtg gaggttgatg tcttccattg gcagaacttg
     1441 tgcataccca ccaccagcag caccaccctt gatccccatg actggaccaa gagacggttc
     1501 gcggatagca atcatggttt tcttgccaat cttgttcaag gcatccgcaa gaccaatggt
     1561 aagcgtcgac tttccttcac ctgcaggtgt tgggttgatg gcagtaacca agatcaattt
     1621 accgactgga ttgctctcaa ctgcacgaat tttatcaaag ctgagtttag ccttgtactt
     1681 tccgtacaac tccaaatcgt cataagaaat accaagtttc tctacaacat caacaattgg
     1741 cttcaactca atactctgtg cgatttcaat atctgttttc attcaaaatt tctctaacct
     1801 cttatatgat aattcattat atcacaaaac aagattttta acatcctaaa actctctaaa
     1861 cgttcgtaaa tatctctgtt tttaaggctt ttagagtcct ttcttaaatt ttatatggct
     1921 ttatagtttg aaactatagt aaatcttcgt ttttaccaaa aatttatcac tttcatttta
     1981 cttaccgctt atttttgtgt acaatagtgc tatgaaaatt ttagttacat cgggcggtac
     2041 cagtgaagct atcgatagcg tccgctctat cactaaccat tctacaggtc acttggggaa
     2101 aattatcaca gagactttgc tttctgcagg gcatgaagtt tgtttaatta cgacaaaacg
     2161 agctctgaag ccagagcctc atcctaacct aagtattcga gaaattacca ataccaagga
     2221 ccttctaata gaaatgcaag aacgtgttca ggattatcag gtcttgatcc actcaatggc
     2281 tgtttctgac tacactcctg tttatatgac agggcttgag gaagttcagg ctagctccaa
     2341 tctaaaagaa tttttaagca agcagaatca tcaggccaag atttcttcaa ctgatgaggt
     2401 tcaggttttg ttccttaaaa agacacccaa aatcatatcc ctagtcaagg aatggaatcc
     2461 cactattcat ctgattggtt tcaaactgct ggttgatgtt accgaagatc atctggttga
     2521 cattgcacga aaaagtctta tcaagaatca agcagattta atcatcgcga atgacctgac
     2581 tcaaatttca gcagatcagc accgagctat atttgttgag aaaaatcagc ttcaaacagt
     2641 ccagactaaa gaagaaattg cagaactcct ccttgaaaaa attcaagcct atcattctta
     2701 gaaaggaaaa ctatggcaaa cattctcttg gctgtaacgg gttcaatcgc ctcttataag
     2761 tcggcagatt tagtcagttc tctaaaaaaa caaggccatc aagtcactgt cttaatgact
     2821 caggctgcta cagagtttat ccaacctttg acactacagg tactctcaca gaatcctgtc
     2881 cacttggatg tcatgaagga accctatcct gatcaggtca atcatatcga acttggaaaa
     2941 aaagcagatt tatttatcgt ggtacctgca actgctaaca ctattgcaaa actagctcac
     3001 ggatttgcgg acaacatggt aaccagtaca gctctagccc taccaagtca tattcccaaa
     3061 ctaatagctc ctgctatgaa tacaaaaatg tatgaccatc cagtaactca gaataatctg
     3121 aaaacattag aaacctacgg ctatcagctg attgctccta aggaatccct actagcttgt
     3181 ggagaccacg gacgaggagc tttagctgac ctcacaatta ttttagaaag aataaaggaa
     3241 actatcgatg aaaaaacgct ctaatattgc acccattgct atcttttttg ctaccatgct
     3301 cgtgatacac tttctgagct cacttatctt taaccttttt ccatttccaa tcaaaccgac
     3361 cattgttcat attcctgtca ttattgccag cattatttat ggtccacgag ttggggttac
     3421 acttggattt ttgatgggat tacttagctt gacggttaac acgattacga ttctaccgac
     3481 aagctacctc ttctctccct tcgtaccaaa cggaaacatc tactcagcta tcattgccat
     3541 cgtcccacgt attttgattg gtttaactcc ttacttagtc tataaactga tgaaaaacaa
     3601 gactggtctg attttagctg gagcccttgg ttccttgaca aatactatct ttgtccttgg
     3661 aggaatcttc ttcctatttg gaaatgttta taatggaaat atccaacttc ttctggcaac
     3721 cgttatctca acaaattcaa ttgctgaatt ggtcatttct gcaattctaa ccctagccat
     3781 tgttccacga ctacaaacct tgaaaaaata aaaacaatct ccgctttcaa acctgaaggt
     3841 ggagattttg ctctatacag tttcttttta atgaaatctt tactatagt
//
LOCUS       DAVBCG010000147         3814 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVBCG010000147 DAVBCG010000000
VERSION     DAVBCG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.147"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            83..1057
                     /gene="folP"
                     /locus_tag="VQ495_001882"
     CDS             83..1057
                     /gene="folP"
                     /locus_tag="VQ495_001882"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2531802.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            1059..2381
                     /locus_tag="VQ495_001883"
     CDS             1059..2381
                     /locus_tag="VQ495_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2531803.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQDTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCRPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            2362..2916
                     /gene="folE"
                     /locus_tag="VQ495_001884"
     CDS             2362..2916
                     /gene="folE"
                     /locus_tag="VQ495_001884"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2531804.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            2959..3771
                     /gene="folK"
                     /locus_tag="VQ495_001885"
     CDS             2959..3771
                     /gene="folK"
                     /locus_tag="VQ495_001885"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2531805.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQVNLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
BASE COUNT         1084 a          742 c          967 g         1021 t
ORIGIN      
        1 aggaaccgac ctctttctac tagagaaaga tgaatgtaat cgtgtccatc tttttctttt
       61 tatggtaaaa tagaaaaata atatgatgaa aatccttgag ggagtgaccg atatgtcaag
      121 taaagccaat catgcaaaga cagttatttg cggaattatc aatgtaaccc cagactcctt
      181 ttcggacggt ggtcaatttt ttgctcttga gcaggcactc cagcaggctc gtaaattgat
      241 agcagaagga gccagtatgc tcgatatcgg cggagaatcg actcggccgg gcagtagcta
      301 tgttgagata gaagaggaaa tccagcgtgt tgttccagtg atcaaagcga ttcgcaagga
      361 aagtgatgtc cttatttcta tcgatacttg gaaaagtcag gtggcagagg ctgctttggc
      421 tgcgggtgcc aatctagtta atgatatcac tggtctcatg ggtgatgaaa aaatggccca
      481 tgtggttgct aaagctggag cgaaagtggt catcatgttt aatccagtta tggcgcgacc
      541 tcagcatcct agttcgctta tcttccctca ttttggcttt ggtcaaacct ttacagaaaa
      601 agagttagct gactttgaaa cattgccaat tgaagaattg atggaggctt tctttgaacg
      661 agcactagcg agagcggcag aagctggtat tgcaccagaa aatatcctgt tggatccagg
      721 aattggcttt ggtctgacca agaaagaaaa tctgcttctt ttacgggacc tggataaact
      781 acatcagaag ggctatccaa tctttctcgg agtgtcgcgc aagcgatttg tcatcaatat
      841 tctagaggag aatggttttg aagtcaatcc tgagacagag cttggtttcc gaaatcggga
      901 cacggcttcg gctcatgtaa ctagtatcgc tgcgagacag ggtgtagaag tggtgcgcgt
      961 gcatgacgta gctagtcaca ggatggcagt tgaaattgcc tctgccattc gtctggctga
     1021 tgaagcggaa aatttagatt taaaacagta taaataagat gaaagaaatt gaaaacaatc
     1081 agtggattgc taactaccgg acggatcaac cgcattttgg cttggaacga atggtggaac
     1141 tgttagcttt gcgtggcaat ccccatctca aactcaaggt tctccatatt ggagggacta
     1201 acggcaaggg ctcgactatt gcttttttga aaaagatgct agaaaagcta gggctgagag
     1261 ttggcgtgtt tagttcgccc tatctcattc attacacaga ccagattagc atcaatgggg
     1321 aatcgatctc agaagcgagg ctagaagctc tcatggcaga ctatcagtct ttgctggagg
     1381 gagaagcggt cgccaattta caggacacaa ccgagtttga gattatcaca gccctggcct
     1441 atgactactt tgcctcagag caagtagatg tggccatcat ggaagttggc atgggcggac
     1501 ttttggatag taccaatgtc tgtcggccca ttttgacagg aattacaact attggcttgg
     1561 atcatgtggc cctacttggt gacaccttgg aggccatagc agagcagaag gcaggtatta
     1621 tcaaacaagg gatgcccttg gtaacagggc gtattgctcc agaagccttg actgtgattg
     1681 accgcattgc ggaagggaaa gatgcgccga gacttgccta cgggacagat tatcaggttc
     1741 gtcatcaaga aagtgtggtg acaggggaag tctttgacta tacaagtgct gtcagacaag
     1801 gtcgcttcca gactagcctg cttggtttgt accaaataga gaatgctggg atggccatag
     1861 ctttacttga tactttttgt caagaagatg gtcgagagct agcaagcaat gactttcttg
     1921 gtcaagcctt ggaagaaaca agttggccag ggcgtttgga aatcgtgtca agagatccct
     1981 tgatgatttt ggatggagcc cataatcccc atgctatcaa ggccttgttg gtaaccttgc
     2041 aagaacgttt tgcggattat cataaggaaa tcctcttcac ttgtatcaaa accaaagcct
     2101 tggaggatat gttggacttg ctgggagcca tgccagatac cgagcttact ctaacacatt
     2161 ttgcggatag tcgggcgacg gatgaaagcg tgctgaaaga ggcagctaag tctagaaatc
     2221 tcagctacca agattggcat gattttctag agcagaattt gacagataaa aaagaagaga
     2281 aacaaacagt taggattgtc acaggttcct tgtatttctt gagccaagtg agggcctatc
     2341 tgatggagag gaagaacgag aatggataca caaaagattg aagcagctgt aaaaatgatt
     2401 atcgaggctg taggagagga cgctaatcgc gagggcttgc aggaaacacc tgctcgtgta
     2461 gcccgtatgt atcaagagat tttttcaggt cttggtcaaa cagcagagga acatttgtca
     2521 aaatcctttg aaattattga cgataatatg gtggtagaaa aggatatctt tttccatacc
     2581 atgtgtgaac accacttctt gccattttat ggtagagcgc acattgccta cattccagat
     2641 ggtcgtgtgg caggcttgtc taagctagcc cgtacggttg aagtttattc gaaaaaacca
     2701 caaattcaag aacgtttgaa tatcgaagtg gccgatgcct tgatggacta tctaggtgct
     2761 aaaggagcct ttgttgtcat tgaggcggaa catatgtgta tgagtatgcg tggtgttaga
     2821 aaaccaggca ctgcaacctt gacgacagta gctcgtggtc tatttgaaac agataaggac
     2881 ctccgcgacc aagcttatcg tttaatgggg ctataaaaag aatccgcttc aagcggattt
     2941 ttctagaaag gaatcattat ggatcaactg cagattaagg atttggaaat gtttgcctat
     3001 catggtcttt ttcctagtga gaaagaattg gggcagaaat ttgtcgtttc agccatccta
     3061 tcctatgata tgaccaaggc agctacagac ttggatttaa cagcctctgt ccattacgga
     3121 gaattgtgtc agcagtggac gacttggttt caggaaacga gtgaagattt gattgaaacg
     3181 gtagcctata aactggtgga acgtaccttt gagtcttatc ctcttgtcca agaaatgaag
     3241 ttggaactga aaaaaccttg ggcaccggtg catttgtcac tagatacttg ctcggtaacc
     3301 attcatcgcc gcaagcaacg agcctttatc gccctaggaa gcaatatggg agataaacaa
     3361 gtaaacttga agcaagccat tgacaaactg cgagctcgtg gcatccatat tctcaaagag
     3421 tccagtgtct tagcgacgga gccttggggt ggagtggagc aggatagctt tgccaatcaa
     3481 gtggttgagg tggaaacctg gctaccagca caagacttgt tagaaacctt gttagccatt
     3541 gagtcagagc tgggacgggt gagagaagtg cattggggac ctcgtttgat tgatttggac
     3601 ttgctctttg tggaggacca gatcctttat acagacgacc tcatattgcc tcatccttac
     3661 atagcggaac gcctttttgt ccttgagtcc ttacaggaaa ttgcgcctca ttttatccat
     3721 ccgatattaa aacaaccgat ccgcaacttg tatgatgctt tgaaaaaata gaaaaactct
     3781 agttttcagt tacttgcaac tgaaggctag agtt
//
LOCUS       DAVBCG010000148         3771 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVBCG010000148 DAVBCG010000000
VERSION     DAVBCG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.148"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..156)
                     /locus_tag="VQ495_001886"
     CDS             complement(<1..156)
                     /locus_tag="VQ495_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2531806.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRK"
     gene            complement(137..322)
                     /locus_tag="VQ495_001887"
     CDS             complement(137..322)
                     /locus_tag="VQ495_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531807.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(626..2188)
                     /gene="guaA"
                     /locus_tag="VQ495_001888"
     CDS             complement(626..2188)
                     /gene="guaA"
                     /locus_tag="VQ495_001888"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2531808.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2330..3028
                     /locus_tag="VQ495_001889"
     CDS             2330..3028
                     /locus_tag="VQ495_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2531809.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(3071..>3771)
                     /locus_tag="VQ495_001890"
     CDS             complement(3071..>3771)
                     /locus_tag="VQ495_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2531810.1"
                     /translation="KDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTATVLQYVCP
                     VGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLFWGLFSALT
                     YALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFLLAFAGIIL
                     IGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDFLGMAMILF
                     AVTLISLKDLFLEK"
BASE COUNT         1195 a          789 c          731 g         1056 t
ORIGIN      
        1 tttacgtcca ccacaaaaga gaaaaacttg acctgagaaa ggatctaatt caaagtgggt
       61 tttaaccaga taagccagtg aatcaatgcc ttgcctcata tccgttttcc cacatacgag
      121 atagacctgc cctaggctag atagatgaat tgtcatagag caacacctta tccaaaagct
      181 gttctaccat ttcgagattg agagattgaa aaaaacttac ttcaagcttg ccaatacgaa
      241 ttttaagcag aatatcattt ctctttttag aatcaaaagg acgagattgt ggaatctcta
      301 caggaacaat aggttgtggc attaaaatat cctccaatag ttttacttta ctaatagtat
      361 acaggaggaa ctccatgaat gatagatacc ttgttatgac gcgattacta tattagaaag
      421 ttggctctct aattaaatta attctcatta tatatatcaa aagaccgcag cttgaaaact
      481 acggtctaat tttttgtcca aatgtaaacc acttggaact attattcaat ttattctttt
      541 tagtaaatgt tcccgtttta atcccagtta cagtttttaa tcctctgtaa ccgttgattt
      601 tacaaagctt ttaatttttt tgcgattatt cccactcaac tgttgcaggt ggtttacttg
      661 taatatcgta gacgatacgg ttaacatgat ccacttcatt tacgatacgt actgagattt
      721 tttgaagtac ttcccatgga attttggcaa aatcagcagt cataccatcg atagaagtga
      781 tagcacggat tgcaatcgtg tagtcatatg tacgaccgtc acccataaca ccgactgaac
      841 gaacgcctgt gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca gctttagcga
      901 tttcttcacg aagaatagcg tctgattcac gaacggtttc aagtttctct tcagtgattt
      961 cacccatgac acggatagca agtcctggtc ctgggaatgg ttggcgccat acgatatggt
     1021 ctggcatacc aagctctgta ccaagagcac gaacttcatc cttgtaaaga gtattgagtg
     1081 gttcaatcaa ttcaaactgc atatcttctg gaagaccacc cacgttgtgg tgtgacttga
     1141 tagtttgagc tgtatccgta ccagactcga taacatctgt atataaagta ccttgagcaa
     1201 ggaatttcac atctttgagc ttgcttgctt cgtcatcgaa tacatagaca aactcgttac
     1261 cgatgatttt acgtttttgt tcaggatcag aaacgccagc aagtttgtca aggaaacgtt
     1321 tagcagcgtc tgctttgacg atattcaaac caaacttacc accgagcatg tccataactt
     1381 gatcagcttc gcctttacga agaagaccgt ggtctacgaa gatacagatc aattgatcgc
     1441 caatcgcttt ttggagaaga accccaacga cagatgagtc aacaccacct gatagaccaa
     1501 gaaggacacg tttatcaccg acggtttcac gaattttttt gatctgcatg tcaatgaaat
     1561 tatccattga ccagtcacct ttagccttac aaatgttaag ggcaaagtta cgaaggatat
     1621 catttccgta tacagaatga cgaacttctg ggtggaattg gataccgtaa atgtgtttat
     1681 ctgggttttc gatggctgcg tatgggcagt cagctgatgt acctgtacga acaaagtcag
     1741 caggaatctc agtaaccgca tcaccatggc tcatcaaaac agtctgttca tcaggtgttg
     1801 attcaaaaag cgctgatggt gtgtgagtta gggttgattg accgtattca cgatttccag
     1861 catcacctgc aggaacaact tttcctccaa gtttatgggt caataactgc ataccataac
     1921 agattcccaa aattggaatt ccgagttcga agatttctgg gtcaatatca aatgaaccat
     1981 cttcatatac agaatttgga ccacctgata gaataattcc tacaggattg acttcacgaa
     2041 cttcagcagc tgaaatttta tggcttttta gttctgaaaa aacaccaatc tcacggatac
     2101 ggcgtgaaat cagctggttg tactggctac catagtccaa tacgatgatt ttttctacat
     2161 cttgcaaatc agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa tatcttttct
     2221 caatattcta tcacaaataa gggcgaatta ccaactaaac aaggcgaagt ttgacttttt
     2281 ctagtttatt ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc
     2341 ttatatgaaa atccatgatc agataaaaaa ggatattgac gagcaccgtt gggctattgg
     2401 tgagaggctt cccagtgaaa gagatttagc tgagcagttt gcggtcagtc gcatgaccct
     2461 ccgccaagcc gtatctctat tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg
     2521 cacctttgtt tccagtactc gagtacaaga aaagatgcga gggacaacca gttttactga
     2581 aattgtcaaa tcccaaggta aagttccctc tagccagctc atttcctaca aaaaaaccat
     2641 tcccaatgag caagaagttg ccaagctagg aatttttcca acggacaata ttatccgaat
     2701 ggagcgggtc cgctatgccg accaagttcc cctagtttat gaagttgctt ctattcctga
     2761 aaaattcatt aaggacttta aaaaagaaga aatcacaagt catttcttcc aaaccttgca
     2821 aaaacatggc tatcgtatcg gcaaatctca acagaccatc tatgctcgcc ttgctaaaga
     2881 aaagattgcc cactatttgg aagttgaaaa aggacatgct attcttggat tgacccaggt
     2941 ttcctaccta gaagatggta ctgcttttga atacgtaaaa agtcaatatg taggcgaacg
     3001 ctttgaattt tatcttgaaa ataattagat taccacgcaa aaactctctg tcacggacaa
     3061 agagtctttt ttatttttct aagaataaat ctttcaaaga aatcaaagtt acagcaaaca
     3121 atatcattgc cataccaaga aaatcaatgg gataaaattg ttcattcatt attaagaagg
     3181 caaagaaaat cgccgatatt ggctcaattg aagccaacaa gcttgacttg accggtccta
     3241 tcagactggc tcctttaagg aaagctgtat aggcaaagac agtcccgata aggataatgc
     3301 ctgcaaacgc aaggagaaaa tcaagactag tcgggatatc ggcctgtaga acccctgtaa
     3361 aaggaagggc gaccaaacct gctatgacca ttcccacacc aatgagcaag ctgctccccc
     3421 actttttaat caaggctatg ggtaaaatga tatacagagc ataagtcaag gcagaaaaga
     3481 gaccccagaa cagaccagca ggtgtcatgg ataactggtc caactgccca tgtgttgcga
     3541 tcaggaaggt tcctccgatg gcgaatatga tggaaactat ctctcccagt gtcggtgcca
     3601 ccctatcctt gatacagcta taaattaaaa ttccgacagg acaaacatac tgaagcaccg
     3661 tcgctgttcc cgcattggtc tcctgaatag cagacagata ggcgaattgg ttgagaaaaa
     3721 gaccaatcag agcaaaaata agaagagaca gcaaactctt tctatccttt a
//
LOCUS       DAVBCG010000149         3751 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVBCG010000149 DAVBCG010000000
VERSION     DAVBCG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.149"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..102
                     /locus_tag="VQ495_001891"
     CDS             <1..102
                     /locus_tag="VQ495_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2531811.1"
                     /translation="VPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            508..1314
                     /locus_tag="VQ495_001892"
     CDS             508..1314
                     /locus_tag="VQ495_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531812.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            1606..2048
                     /locus_tag="VQ495_001893"
                     /pseudo
     CDS             1606..2048
                     /locus_tag="VQ495_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2438..2647
                     /locus_tag="VQ495_001894"
     CDS             2438..2647
                     /locus_tag="VQ495_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531813.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            2637..3125
                     /locus_tag="VQ495_001895"
     CDS             2637..3125
                     /locus_tag="VQ495_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531814.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            3241..>3751
                     /locus_tag="VQ495_001896"
     CDS             3241..>3751
                     /locus_tag="VQ495_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-diphosphate and nicotinate from nicotinate
                     D-ribonucleotide and diphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2531815.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWG"
BASE COUNT         1229 a          543 c          707 g         1272 t
ORIGIN      
        1 gtcccacaac ccgttcaatc aggagacatg gttcgagctc ttaaagaggg gcttatcaat
       61 ctttataagg aagatggaac cagcgtcaca gttcgtgctt aatgtagttg tttagtttta
      121 aaaaattatg caaagctcca tatacaacac ttaaacgaga ttaaagaatg gcgaaatccc
      181 ttgatgcgca agagattagc tgtctttttt attttttaag tgataaagtc ggagcttagg
      241 tatcaaagcc tatcaaatta aacaaagaag cgatgtctta gatattttga aaaaaattaa
      301 taagcagaaa actctctatt attttgttgt agagagtttt ttgttaataa aattttacaa
      361 aatgacattt atatattgca tcaagttaga tatatgatat aatattgtta aaaagaggcg
      421 caacttttta aactcaataa gaatcaaaga gtaaaccatg aatattaatg taggaataaa
      481 aatgtaagta agcattatgg tcattcaatc attctcaaag atataaattt tgcacttaac
      541 aagggtgaaa ttgttggtct agtagggaga aatggagttg gtaagagtac gttgatgaaa
      601 attcttgttc aaaataatca accgacttca ggtaatatta taagcagtga taatgttggg
      661 tatttaatcg aagaaccaaa attattttta tctaaaacag gtttagagaa tttaaaatat
      721 ttgtcaaatt tatatggtgt tgactacaat caagaaagat ttagaagttt gatccaagag
      781 ttagatttga ctcagtctat taataaaaaa gtaaagacct attctttggg tacaaaacaa
      841 aaattagctt tgcttctaac tctcgttacg gaacctgata tattgatttt agatgaaccg
      901 actaatggtt tagatattga atcatcacaa atagttttag cggttctaaa aaaattagct
      961 ttaaatgaaa acgtgggaat tttaatatcg agtcataaat tagaagacat tgaagaaatt
     1021 tgtgagagag ttcttttctt ggagaacggg cttttgacat ttcaaaaagt aggaaaagat
     1081 agtcataatt tcttgtttga gatagctttt tcatcagcta cagatagaga cattttcatt
     1141 accaaacaag aatttgggga tattgttcag gaagagggat tgagaattac tatgtctggg
     1201 aatattcaaa gtagtgagct ttttaaattt tttaacgaaa actctattaa agtagttgat
     1261 tttgaaacta aaaaagagac gcttaaagat atttacctaa atcgttcaaa ataaaggaag
     1321 gttataatca tgaaattaaa taaattgaat tttcttaagg aaaatataag aaatttatat
     1381 tcatcaggcg taatatatct cagtttgctt atctcgttta taccgccgat attgcttaca
     1441 ttctttattc taaagactta agggacatcg cttggtatta agcatatttc aaacttttat
     1501 gctatgctcg gtatgttaat ggctgttata catgctaatc gagtcattag tagagatttt
     1561 tcccacaata cggtaagttt gttttataat caacagaaga atcggatgat ttacgtcttg
     1621 tctaattttc tatatgccat ctcagcttcc attatttatg ctttgaatgg cattgtgtta
     1681 ctagtcatag taagtaaatt gggtattcca ggtgatttag gattagattt tatagtagct
     1741 attgtagtca atacaatttt gttagtcctg ttttattttc tattatctta cattttctat
     1801 ttatacaaat tgaaaagtgg cttggtattt tagtagcttt actactcttt atccctaata
     1861 tattaaatac gatgatgaat actagtaatg atttgtttat caaagcaatt gaacttcttc
     1921 ctttttattc tttacctgta tttgtggctt caaatacgat gtctattagt cagtatcttg
     1981 tggcaatcac tacaatcatt ttattgtact ttttcactct caagaaaagc aagaagtatt
     2041 cattttagtt tcatttagta ttattttgca tacctaaaat acagtaaaaa atcagtcata
     2101 ttggtatgct cctgctttca ctattcaaca cgtttttgac ttatactagg ctcatttcca
     2161 aaagcattat ataatagtga tatgaaacca actaaactaa acaagaaata taagcaataa
     2221 aaactcgttt aaaagatcct actaaagcta atgctaaata aaaataaaaa taaaagagta
     2281 aactaggaag tttatttcaa acaacctaaa atactgattt tcggctgaag ataatactgg
     2341 agtgcaaatt aatggggtta taataaatag ctgacagctt gtgttagttt ggatttttta
     2401 agagtagatg agtattaaaa ctataaggag gacgaaggtg gctaaaaatt taaaattaaa
     2461 attagctcgg gtagagcgtg atttaacaca aggtcaactg gcagaggctg tcggggtgac
     2521 acgccagact attggtttga tagaggcggg aaaatacaat cccagtctct cgctctgcca
     2581 gtctatttgc agatgtttag ggaaaaccct agaccaacta ttttgggagg aagaagatga
     2641 aaaatagatt ttattattat caattactag acgaaagaga agaacaactg atcaataaag
     2701 cgggctctga aagtttctat atctgcatcg ctttgtcgct cctatcttat atcatttcag
     2761 tattagcacc aagccttttt aattctaata tgctgctaat cgttatcatc atagggacat
     2821 tttacttttt taaccgtgcc cgttatctgg gagtgaccta ctatagtcgt tttcatttta
     2881 cgattttggg ttgttttttc ctaaccttgg ctattacggc tcttttgatg ttgcagaatt
     2941 atcaattcaa catagaaatt tatcagcaca atcctttgaa ttttaaatac ctgtctgctt
     3001 gggtcattac ttatgtcatt taccttccgt gggtctttat tggcaatctt ggtcttaaga
     3061 gctatggcga atgggctcag aaaaaatttg aacaagatat ggatgaattg gagagtggag
     3121 aatagcttgt tactcttttc tcaatccagc taaaatgtga tataatagta ctaatttatt
     3181 ggaatacatg aaagttcttg aaaattttca tgggtttcta gctaaggaag taggaaaagt
     3241 atgtatccag atgatagttt gacattgcac acggacttgt accagatcaa catgatgcag
     3301 gtttactttg accaagggat tcacaataag aaggcggtct ttgaggtgta tttccgccaa
     3361 cagcctttta agaacggcta tgcggttttt gcaggtttgg aaagaattgt gaactatctt
     3421 gaagacttgc gtttttcaga tagtgatata gcctatttgg agtcgcttgg ttatcatggg
     3481 gcgttcttgg attaccttcg caatttcaag ttggagttga ccgttcgttc tgcccaagaa
     3541 ggggatttgg tttttgctaa tgaaccgatt gtgcaggtgg aaggacctct agcccaatgt
     3601 cagttggtcg aaacggctct tttgaacatc gtcaactacc agaccttggt ggcgacgaag
     3661 gcagctcgta ttcgttcggt tatcgaagat gaacccttga tggagtttgg gacacgtcgg
     3721 gctcaagaaa tggatgcggc catctgggga a
//
LOCUS       DAVBCG010000150         3695 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVBCG010000150 DAVBCG010000000
VERSION     DAVBCG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.150"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..1403)
                     /locus_tag="VQ495_001897"
     CDS             complement(<1..1403)
                     /locus_tag="VQ495_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HET2531816.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGTGLDSVYHVPIYYKLTVTNDGSKLTFTYTVTYVDPTTRALGNLSKMGHG
                     YSIYNTGTSIQTMLTLGSDLGTPSDVKNYITDKDGVLVRSYNTSTMTTWGSGYTWGNG
                     AQMNGFFAKQGYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFKGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASESASTSASASASTSASASASTSASASASTSA
                     SASASTSASESASTSASA"
     gene            complement(1401..1514)
                     /locus_tag="VQ495_001898"
                     /pseudo
     CDS             complement(1401..1514)
                     /locus_tag="VQ495_001898"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(1752..2213)
                     /locus_tag="VQ495_001899"
     CDS             complement(1752..2213)
                     /locus_tag="VQ495_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531817.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(2180..2323)
                     /locus_tag="VQ495_001900"
     CDS             complement(2180..2323)
                     /locus_tag="VQ495_001900"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2531818.1"
                     /translation="MAYSTDFKQGALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KGT"
     gene            2592..2972
                     /locus_tag="VQ495_001901"
     CDS             2592..2972
                     /locus_tag="VQ495_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2531819.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            3071..3292
                     /locus_tag="VQ495_001902"
     CDS             3071..3292
                     /locus_tag="VQ495_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2531820.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            3309..3623
                     /gene="trxA"
                     /locus_tag="VQ495_001903"
     CDS             3309..3623
                     /gene="trxA"
                     /locus_tag="VQ495_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2531821.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
BASE COUNT         1110 a          751 c          713 g         1121 t
ORIGIN      
        1 gctgacgcac tcgttgatgc agattcagac gctgaggtac ttgctgaagc tgacgcactc
       61 gttgatgcgg aagctgacgc actcgttgat gctgaggctg aggcacttgt acttgctgag
      121 gctgatgcgc tggttgaggc cgattcagaa gctgaggtgc ttgctgaggc tgaggcgctt
      181 tgactagcac ttactgagag tgacttagac tgtgaaagtg actgactggt cgtgctagat
      241 tcaactacct ttcctctacc cttgaagtag ttagttctaa tgttatctgt tttagcagcg
      301 taaggggtaa atgtaaagga tgtatccgtt ccagtaattg gtacagtcca agatgatgtt
      361 aatccatatc cttgcttagc aaagaaacca ttcatttggg caccatttcc ccaagtatac
      421 ccactacccc acgtcgtcat tgtagatgta ttataggatc gaaccagtac accatctttg
      481 tcagtaatgt agttctttac atctgaaggg gtaccaagat cactgccaag ggttaacatt
      541 gtttgtattg aagtacctgt attatagata gaatacccat gacccatctt tgaaagatta
      601 ccaagagccc ttgtggtagg atctacatac gtaaccgtat aggtaaaggt cagttttgaa
      661 ccatcatttg tcactgtcaa tttatagtaa attggaacat gatataccga atctaaacct
      721 gtacctgtat aaacttttgt ctcactttta gcaatgttta gacttgcatt aattgcaggg
      781 gcgccattta caatcgtatt gccagaaaaa acagcagctt ttatagaagc cagcaattgc
      841 tcgatggaat ccactgaacg cttgcgtcgc ttagcataag attggagatt gacatttgta
      901 actgatgctg cataatcact agctggtttc ttacgatctt cttcgacctt cttagcagtt
      961 gcttctgtag cggcagctgt ttgtgatcct accacagtag atgatgcaga gatacttgtc
     1021 gaagccgatg tacttgctga ttctgatgca cttgtactcg ctgaggtcga agcgctggtt
     1081 gaggctgact cagatgcaga tgtacttgcc gactctgaag ctgacaaact agttgaactc
     1141 gctgaattac ttgtagatat cgtacctaga actactgtat catttgttgc caaagcatcc
     1201 gttttctcta cagttttttc aagtactgcc gactcatttg taaatacctt cgtttgagtt
     1261 gcaacggttc cacttatgac agttcccgca gcaactattc ccttgaggat atcaagccca
     1321 gtaattgaat gacttacttt atcttctacc gtttcggtca tgacctgagt agtatcaaca
     1381 ccaccacgca tgaccttaaa caagccaaaa agagaagtcg aggcccgtag ccaatgcttc
     1441 cctgatttga ttaatttgaa ccgagttact cggtctgttt ctctatattc acccttctga
     1501 cgtctaaaaa atattttgaa atttcctttc gagtattatt ttcatgtatg gatataaata
     1561 aattaccata ctataattat agaataggga acccaaaata gcaagaaact tgaggtattt
     1621 ttgctaaaaa aaaaaagcat tttactattt tatatatata ttataagata agttttttat
     1681 aaagacacgc aaatgagtat aaatcagcta atttttggtt tatattccta tttttatgca
     1741 taattattct ttcattctct aatttgtgaa tcaatatatc tgattagaaa actaccattg
     1801 agtttttgct ccacctcatt agtccgctca aaacgccgtc cgctaatctt gtcatagact
     1861 ttctcccctc taggagcccc tgcataagga cgatagaggt agcggttgat tcccgtttcg
     1921 tcaatatata ggactggtaa atcttttagg ttatccaaaa tatcaagaaa ctcagaaact
     1981 ttctcaggct tttgttccct aaaactggtc gtctttttta aaatgacctt aatctgcttt
     2041 aaaactgccc atacggaggg caaagcacaa tcaaaacggg ccgcaatttc ccgtaaaaaa
     2101 gcgtctggat gagcctctac aaaggctttc aattcttcta aagggatctt acgctttttg
     2161 acgactcgct ttttccgctc taagttccct tgtttacgtc tttcttttcc cacgtgaaga
     2221 gagttctgac gccaacacca aaaaacttgg ctgcctcgac atgactgtgc ccctctttga
     2281 tggaatctaa tgctccttgt ttaaaatcag tactatatgc catagcttta ttagaccatg
     2341 cttattttaa actaagcaac tatagataga gaaatgaaat aaaatctaaa ttcgaatgtg
     2401 ataaggagtt agaaattgaa acaataggat tcccccatta ttcacacaaa aataaaacca
     2461 ctaaaaaaca gagcttgcca gcattcttga aaagtagtat aattattgca tgcgcaatca
     2521 tcttgtgacg cagagactgc ccgttaatgg actttcttct atattatgac tctaaaaaga
     2581 aaggagacac tatgacctat ttggaaaaat ggtttgactt caatcggcgt cagaaagaaa
     2641 ttgaaagtct cttggaagag accattgccc aacagagcga gcaaagtctg accttgaaag
     2701 agttctacct gctctactat ctggacttgg ctgaagaaaa atctctacgc cagattgacc
     2761 tgccagataa acttcatctg agcccgagcg cagtttctag gatggtggct cgcttggaag
     2821 ctaaaaattg cggtctactc agtcgcatgt gttgccatca agatagacgc tctagtttta
     2881 tctgcctgac aaatgatggg caaaagacac tggcctcgct acaaaagact gtcgaagaaa
     2941 gcttggaagc aggtttggat ttcttgattt aagatgattt taaaaaaagt tgcgtgcgca
     3001 atcatttttc ttgacattct cttttataag gagtaaaata aagttagcat taaacaaagg
     3061 agttttaaat atgattgaaa ttacctatat agatgccagc aagaacgaaa gaactgtgac
     3121 tttcgagtct tatgaagact ttgaacgttc acaacaagct tgccttatcg gcgtcgcaga
     3181 ctactaccct gtccaaaaat taacttacaa gggtcataat ttggactacc atgggactta
     3241 tggagatatc ttcttctatc tcatgaaaca agatttaagc caatataact aaaaaaagga
     3301 gaaatacaat ggcaaaagca attacagatg caacattcga acaagaaaca aaagacggtt
     3361 tggtcttagt agacttctgg gcaacttggt gtggtccatg tcgtatgcaa ggtccaatct
     3421 tggacaaatt gtctgaagaa ctttcagaag atgtcttgaa aatcgttaaa atggacgttg
     3481 atgaaaatcc aaacacagct cgtgcttttg gaatcatgtc tattccaact cttctcttca
     3541 aaaaagacgg ccaagttgtc aaacaagttg caggtgttca cacagcagaa caaatcaagg
     3601 ccatcattgc tgaattgagc taatcaaacg agagaccaag tactttcttt ggtctctttt
     3661 ttcttgccct ttgccatttt tcaaaaaata tgcta
//
LOCUS       DAVBCG010000151         3633 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVBCG010000151 DAVBCG010000000
VERSION     DAVBCG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.151"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(111..1862)
                     /locus_tag="VQ495_001904"
     CDS             complement(111..1862)
                     /locus_tag="VQ495_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531822.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(1852..3597)
                     /locus_tag="VQ495_001905"
     CDS             complement(1852..3597)
                     /locus_tag="VQ495_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2531823.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGYDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
BASE COUNT         1109 a          809 c          805 g          910 t
ORIGIN      
        1 aaaggcggtg aaaaaaatct gtaatttgaa ggatttcctt ctagtactca atgaaaatca
       61 aagtacagct ttgagattgc agataaagct gacatggttt aaagagattt tcaaagagta
      121 taggccatgg cccctgcctg caaactatac atcttgtgat aggttcctcc cagagccaag
      181 agttcctcat gggttccact ctcgataatg cgtcccttat ccaagacata gatgcagttg
      241 gcgtcttgaa tagtagaaag gcggtgagct atagcaatag ttgttcggcc ctgtctcatc
      301 ttcgccagag aagcttgaac caagctttct gtttcagagt caatattggc tgtcgcttca
      361 tccaaaatca ggattttagg ctggctggcg actgttctag caaaggcaag aagctggcgt
      421 tgcccagtag agaagctcga accacgctcg gaaacagggg agtcgtaccc ctgaggaagt
      481 tcttgaataa aggaatctgc atccacaaag gctgccgcag cctgaacctg ctcatcactg
      541 atttcttggt acatggcgat attggactta atagttccat gatagaggaa gggttcctgc
      601 aagaccaaac cgatgttttt tctcagctct tcttgactga aatccctgat atccacatca
      661 tccaagagaa ctctccctga ctggaattca taaaagcgca tgaggacatt gataatagac
      721 gatttccctg aacctgtatg acctacaaag gcaatggttt cacccttatt aacagagaaa
      781 gaaatgtcat ccagaatcgg atgtttaccg tcatatgaga aacacacatg ttcaaagcgg
      841 atattgcctt cttggacttt ggcttgccca ttttcttgaa gaggttcata ggtcctctcg
      901 tctaccaggg caaagacacg acctgcagaa accatagacg tttgcagtgt tgaaaagttt
      961 tgcgtcacct caatcaaagg gtcaaagagg cggttgatgt actggataaa agcatacatg
     1021 gtcccggccg ttatcccgat agaaagacca cgatagccaa aataggccat caagacagca
     1081 taacctagaa gtttcaacaa actcatggca ggtctcaaaa agagggcatc caaggctaca
     1141 gaacggttgg catagaccaa gtgctcttgg ttgatttcat caaattctgc ctgcaggcgc
     1201 ttctcttgat taaaggcctg gattattctg attccctcga tgttttcagc cagcttgcta
     1261 ttgatatctg acaagagact tctggttttc tcaatgattt tcactgattt tttccgatag
     1321 agattgacca aaaggaaaat caaggggaga aagagcaaga ctaatgctgt caaacgaaaa
     1381 tccaatacca acatggtata aagggttgtc agaaagatga aaactgctga gataaagcta
     1441 gataaaatcc cagaaaacat atcactaatc gtctcggtat cgttggtcaa acgagaaacg
     1501 atagaacctg ctggcgtctt gtcaaagtaa gacatgccca gtttctccat attggcaaag
     1561 gcatcccgac gaatatccct aacaatactg taagacacgc gcgcaaagag aagattgccg
     1621 acatactgaa ctacagtttg taggatgtag agaccatagt agaccagcaa aacggtaacg
     1681 gctagttggt taagattgct gagatactgg tcgataaagt gggaagccac gaggggtatg
     1741 acacttttaa tgaccgtcgt cgctaggaga aaactgagtg ccaaaaaggt caggagtcta
     1801 taaggcttga gataagacat caagcgcttc aatacagccc attgttcttg tttattctgc
     1861 atcttcttct cctttcattt ctaactgctg agactggtag gtttgggcat accagccatc
     1921 catcgctagc aagtcttcgt gcctgcctcg ttcgataatt tgaccatttt gtagaactaa
     1981 aataaaatct gcatggacaa cagcactgag gcgatgggca gttataatgg ttgtcttgtc
     2041 ctttcgcgtc tccttgagat tgtcgataat cgcatactct gtcttggcat ctacggcgga
     2101 taaggaatca tccaaaatca agatatcagg gtttaaaatc atagcccgac tcatagccaa
     2161 ccgttgcttt tgaccaccag aaagactgac tcctttttca ccaatcagcg tatcaaatcc
     2221 ttgaggcatg tctacaatat cttggtaaac tcgggctagc ttagtagctt cctcaacctc
     2281 tgaaaggggc aagttaggat tgccaaagcg gatattgtct aggattgaag tcgcaaagag
     2341 aaactggtcc tgaggaacat agcccatgag actgcgaagt tctgtcaaac gatagtcccg
     2401 aatatcgtaa ccgtttagat aaatggcacc cttatccaca tcgtattcac gcaagaggag
     2461 cttgattaag gacgttttcc cagagcctgt ctgcccaacc aagcccagtg tttgcccttt
     2521 tgccaaacta aagtgaatat ccgtcagtgt ttcctcattt tcaaaagcaa agctgtcaat
     2581 ggcatactcc aaacgcccat tttcaatacc atccagagga aactcagggt cttgtacagg
     2641 agattcctga gacaaaagat tttcaatccg ctggtaagaa accttccctc gctgagtagt
     2701 attaaagagg aagccgatgg ccataagagg ccagaccagc atatccaaat agctgataaa
     2761 ggtgactaga ttaccaactg taatctgccc ttcctgaacc atcaaggagc caaccaaaag
     2821 cgttaaaaca taggacgaac caacaaacaa gagaaccata gggtcaaaga gactatcata
     2881 tttcatggtt tgcaggttct tttggaaggt taattcattg actgcctgaa aagacttcaa
     2941 ctcgtctgcc tgataaccga aagacttggt cactttgata cctgatacgg actcctgtac
     3001 cttgttattg agttcagaaa aagcagcttg ggattcgcca aaggccttat gagtctttct
     3061 ccctaggcga ctagtcgcat aggccatgaa aggtaggggg agaatggcaa caagagtcat
     3121 ctgccatgag atgctaaaga gcatggtcaa caaagtcacc agagccgtga tagaggcatc
     3181 caccgcagac atgacaccgc cacctgctaa acgagtcaag gcattgatat cattggttgc
     3241 gtgtgccatc agatcacccg tccgataggt ttgataaaag gctggcgaca tttttgtgaa
     3301 atgcttaaac aagcgagacc gcatgatctg tcccaagcaa taagaggtcc caaggatata
     3361 catacgccac acatagcgca aatagtacat accaaaggct gcaagtagca agtaaaatag
     3421 gctaagaagg aggtcctgct gggttaattg ccccgatgtg atggcatcaa tgactcgccc
     3481 cataaccata ggaggaatga gattgagaac ggaaaccaag accagggcca caatcccgac
     3541 tagataacgg tgtttttcta acttgaaaaa ccaccagagt ttttgaataa tggacataaa
     3601 atccctttct gattgcaaat ggaaacctga ggc
//
LOCUS       DAVBCG010000152         3621 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVBCG010000152 DAVBCG010000000
VERSION     DAVBCG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.152"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(1..801)
                     /locus_tag="VQ495_001906"
     CDS             complement(1..801)
                     /locus_tag="VQ495_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2531824.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            972..1577
                     /locus_tag="VQ495_001907"
     CDS             972..1577
                     /locus_tag="VQ495_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2531825.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            1590..2990
                     /gene="pepV"
                     /locus_tag="VQ495_001908"
     CDS             1590..2990
                     /gene="pepV"
                     /locus_tag="VQ495_001908"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2531826.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            3044..>3621
                     /locus_tag="VQ495_001909"
     CDS             3044..>3621
                     /locus_tag="VQ495_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2531827.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
BASE COUNT         1079 a          742 c          798 g         1002 t
ORIGIN      
        1 ttattgtaac tgagattgat ctgggaggta agaaccacct agataggtat tgctgagttt
       61 ttcaagggtt ccgtcttgat agagttcttt gagtgcttta tcaaattgct ctttaaactc
      121 tttttggtcg cttgagaaaa tgatataatt gctggggcta tctgcagaag gtaaatcaac
      181 gactgagagg tctaaaccac ggtccttgat aatcttttga acggatacct tgtcaaaaac
      241 taggaaatca aactctccgt tagcaaggtc taggattcgt ttaccaatat cctcaccaga
      301 aaaattaatt gtagcgggat tatcagtgtg tttctgattc cagttattga tgaattgagc
      361 gttagaagtt ccggtatcct cttgtgttgt tttaccagcg atctggtcaa gagaagtcaa
      421 aggatttttc ttgttgctga caaggacgag gggattgttg gaaatcggaa gcgagtaaag
      481 gtatttttca gcacgctctt ttgtgtaact caaattattg gccgcagcct gatagtgacc
      541 agaatcaagt cctgggaaga tactttccca ggcggttctt tgaaattgaa tctcgtagtc
      601 gctgagtttt tcatctactg cctttagaac ttcgatatcg aagcctgtca gattgccttt
      661 atcttcgtag tcaaatggtg gcacgtcgcc agctgtagca aggacgattg tcttttgagc
      721 gctagtctct ttgggtgtag cttgattccc acaggcaacc aaaaatggta ggatagctag
      781 taataggcta aattttttca tactgtctcc attcaaatgt aaagtacttg ctagtttatc
      841 agaaactttc ctgataaacc aatatatatc ttttatgtct gtgatagaaa aacttaatca
      901 tgaaaaatcc ctgcaagctc tttcaaatta tggtagaatg gaagcagtag aattagaaaa
      961 ggaaatcgat tatgaaattt cttgaattaa ataaaaaacg tcatgcgacc aagcatttca
     1021 ctgataagcc tgttgatcta aaagatgtgc gtacggctat cgaaattgca accttggcgc
     1081 caagcgccca caacagccag ccttggaaat ttgtagttgt tcgtgagaaa aatgctgaac
     1141 tggcaaagtt ggcttatggt tcaaactttg agcaggtatc atcagcacct gtaaccattg
     1201 ccctatttac agatacagac cttgccaaac gcgctcgtaa gattgcccga gttggtggtg
     1261 ctaacaattt ctcagaagaa caacttcaat acttcatgaa aaatttgcca gctgaatttg
     1321 cacgctacaa tgaacaacaa gtcagcgact acctagcact caatgcaggt ttggttgcta
     1381 tgaacttggt tctcgctctt acagaccaag gaattggttc caatattatc cttggttttg
     1441 acaaatcaaa agtcaatgag gttttggaaa ttgaagaccg tttccgccca gaactcttga
     1501 tcacagtggg ttacacagac gacaaattgg aaccaagcta ccgcttgcca gtagatgaaa
     1561 tcatcgaaaa aagataggaa gaagaaaaaa tgatagcaat tgattttaca gcagaagtag
     1621 aaaaacgcaa agaagacctc ttggctgact tgtttagcct tttggaaatc aactcagaac
     1681 gtgatgatag caaggctgat gcccaacatc catttggacc tggtccagta aaagccttgg
     1741 aaaaattcct tgaaatcgca gaccgtgatg gttatccaac taaaaatgtt gataactatg
     1801 caggacattt tgagtttggt gatggagaag aagttctcgg aatctttgcc catatggacg
     1861 tggttccagc tggtagcggt tgggacacag acccttacac accaactatc aaagatggtc
     1921 gcctttatgc gcgtggtgct tcagatgaca agggacctac aacagcttgc tactatggtt
     1981 tgaaaatcat caaagaattg ggccttccaa cttctaagaa agttcgcttc atcgttggaa
     2041 cagacgaaga atcaggctgg gcagatatgg actactactt cgagcacgta ggactcgcca
     2101 agccagactt tggtttctca ccagatgctg aatttccaat catcaatggt gaaaaaggaa
     2161 acatcacgga atacctccac tttgcaggtg aaaatacagg tgcagcccgt cttcacagct
     2221 ttacaggtgg tttacgtgaa aatatggtac cagaatcagc aacagcagtc gtttcaggtg
     2281 acttgacaga cttgcaagct aaactagatg cctttgttgc agaacacaaa cttagaggag
     2341 aactccaaga agaagctggc caatacaagg tgacgatcat tggtaaatca gcccacggtg
     2401 ctatgcctgc ttcaggtgtc aatggcgcaa cttaccttgc cctcttcctc agccagtttg
     2461 gctttgctgg tccagccaaa gactaccttg acatcgcagg taaaattctc ttgaacgatc
     2521 atgagggtga aaatctcaag attgctcatg tggatgaaaa gatgggtgct ctttctatga
     2581 atgccggcgt cttccgcttc gatgaaacaa gtgctgataa taccattgcc cttaacatcc
     2641 gctatccaaa aggaacaagt ccagaacaaa tcaagtcaat cctagaaaac ttgccagttg
     2701 cttctgtcag tctatcagaa catggtcata cccctcacta tgtgccaatg gaagatccac
     2761 ttgtgcaaac cttgttgaat gtctatgaaa aacaaacagg ccttaaaggt catgaacaag
     2821 tcatcggtgg tggaaccttt ggtcgcttgt tagagcgcgg agttgcctat ggtgctatgt
     2881 tcccagactc aattgatacc atgcaccaag ccaatgaatt tattgccttg gacgatctct
     2941 tccgagcagc agcaatttat gccgaagcta tttacgaatt gatcaaataa aacgatagaa
     3001 gtctgagatc ttatgcttgg acttcttttt ggagggaaag tagatgtctc aaatcgaaag
     3061 aatcaaacag gctatcatgg cggattcgca gaatgccagc tatacagagc gtggcattga
     3121 gcctctcttt gcagcgccaa aaactgctcg catcaatatc atcggtcagg ctccgggact
     3181 taaaactcaa gaagcaggcc tttactggaa agataaaagt ggtgaccgtt tgcgggactg
     3241 gctaggtgtg gatgaagata ccttttacaa ttcaggttat tttgctgttt tgcctatgga
     3301 tttctacttt ccaggacatg gcaagtcggg tgatcttccg ccttgtacag gttttgcaga
     3361 aaaatggcat ccgcagctct tacaggaatt gcctgatatt cagttaaccc tcttgattgg
     3421 gcaatatgcc caagcctact atttacagga gaaaatcagt gggaaggtaa cggagagggt
     3481 gaaacactat aaagactatc tgccagccta ttttccgcta gttcacccat caccacgaaa
     3541 tcaaatctgg atggccaaaa atccttggtt tgaggcagaa gtagtgccag atttgaaaaa
     3601 aagaattaaa accattttat a
//
LOCUS       DAVBCG010000153         3582 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVBCG010000153 DAVBCG010000000
VERSION     DAVBCG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.153"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            94..954
                     /locus_tag="VQ495_001910"
     CDS             94..954
                     /locus_tag="VQ495_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2531828.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            1165..1875
                     /gene="ccdA2"
                     /locus_tag="VQ495_001911"
     CDS             1165..1875
                     /gene="ccdA2"
                     /locus_tag="VQ495_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2531829.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            1895..2461
                     /locus_tag="VQ495_001912"
     CDS             1895..2461
                     /locus_tag="VQ495_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2531830.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            2472..>3582
                     /gene="msrB"
                     /locus_tag="VQ495_001913"
     CDS             2472..>3582
                     /gene="msrB"
                     /locus_tag="VQ495_001913"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2531831.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT          986 a          661 c          795 g         1140 t
ORIGIN      
        1 cctcaaagca gggctttgag caacctgcgg ctagcttcct agtttgctct ttgattttca
       61 ttgagtataa gattataaat gaaggagtat gacatgaaaa agtggtggaa agagctgata
      121 gacaagcctt tattaaaagc ttttttgcat tattatcaag catcagatag tgagttgacc
      181 agtgtcgcag tagcctacta ttggttgatt tcgattttcc ccctgctttt ggtggtggtc
      241 aatatcctcc cctattttca gattcctgtg ggagaatttc tgtgctttat gaaagacgtt
      301 ctgcccccaa gcctctatga aggtgtggaa aaaatagcca gagaagtttt gacgcaacct
      361 tcaacaggtt tattgagttt ttctgtttta tcggcgttat ggagtttttc aaaatctatg
      421 aatttcttgc aaaaagcttt taacaaggct tatggcgtcg aaaagagtcg tggacttatt
      481 tcccatcaga tgctaagtct cctagtcagt tttggcttgc agctcctctt tgcgtttgcc
      541 ttgtttttga tcttatttgg tcagatgatt ttggctttac ttgctcatta ttggacaaaa
      601 gatggcatta tctatcaggc tttacaaggg ttggcaggtc ctctgattta cgccctcctc
      661 tttgctatct tggtcatgct ttattatttt cttcccaatc tatctaacag gaaaattagc
      721 tataccctac caggcagtgc ctttgttctc ttagtgattt taggtttact aactctgttc
      781 tctagttatc tcaattacta tgtccatcat ttggtggatg tccgaatttt aggttccgtt
      841 cttcttgttg ttatgatgtt ttggtttatc ttgattgcta aaattgttat cctaggcgcg
      901 gttatcaatg ccagtatgca gagtttgaaa gatccggtct ttaaaaaaga ctaactattt
      961 atccattaca aaagcgcata gtatcaaaac tttatagaac cttgatacta tgcgcttttg
     1021 tgctttgaca gttttttgta tagtttttgt ccaagatttc taaatcatct gattaaattc
     1081 ctattttccc ctatcattgt ccctgttttt tcgaaaatgg aaggagtata ataaatctat
     1141 caataaaatg ataggaggaa ctttatgggt catattttct tttttctaag tgtctttttg
     1201 gcagggattc tatccttctt ttctccttgt atcttacctt tgttaccggt ctatacagga
     1261 gtgttactag atgataagga tggtgctcag gcttctagcg gcaaattttc aatctcagtt
     1321 actagtttat tacgaactct ggcctttata gcaggaattt cctttatatt tattttgttg
     1381 ggctatggag ctggtttttt aggcgatttg ctttatgctt cttggttcca atatcttact
     1441 ggggcaatta ttatccttct tggtttgcac caaatggaga ttctacactt taaggggctt
     1501 tataaggaaa agaggctaca actgcaagga caggggcaaa atggtaaggg ctatagtcag
     1561 gcatttttat tgggcttgac ctttagtttt gcttggacgc cttgcgtggg gccggttctg
     1621 gggtctgttt tggccttggc ggcttcaggt ggttcaggag cttggcaggg agctggtctc
     1681 atgttggtgt atacgctggg cttggcgcta ccattcttgc ttctagctct gacctctagt
     1741 tatgttttga aacatttccg aaaacttcat ccctatctcg gaatcctcaa aaaagtgggt
     1801 ggttttctca ttattgtgat gggactcttg gttctgtttg gaaatgcttc aattttaagt
     1861 caattatttg aataaaatgg aaaggaatat caatatgaaa aaatggcaaa catgtgttct
     1921 tggagcaggt tcgctccttt gtttgacggc ttgttcaggc aagtccgtga ctagtgaaca
     1981 ccaaacgaaa gatgaaatga agacggagca gacagctagt aaaacaagcg cagctaaagg
     2041 gaaagaggtg gctgattttg aattgatggg agtagatggc aagacctacc gtttatctga
     2101 ttacaagggc aagaaagtct atctcaaatt ctgggcttct tggtgttcca tctgtctggc
     2161 tagtcttcca gatacggatg agattgctaa agaagctggt gatgactatg tggtcttgac
     2221 agtagtgtca ccaggacata agggagagca atctgaagcg gactttaaga attggtataa
     2281 gggattggat tataaaaatc tcccagtcct agttgaccca tcaggcaaac ttttggaaac
     2341 ttatggtgtc cgttcttacc caacccaagc ctttatagac aaagaaggca agctggtcaa
     2401 aacacatcca ggattcatgg aaaaagatgc aattttgcaa actttgaagg aattagccta
     2461 ggaggtgtct tatgaatgat aagttaaaaa tcttcttgtt gctaggagta ttttttctag
     2521 ccataaccgg tttctatgtt ctattgatac gaaatgcagg gcagacagat gcctcgcaaa
     2581 ttgaaaaggc ggcagttagc caaggaggaa aagcagtgaa aaaaacagaa attagtaaag
     2641 acgcagactt gcacgaaatt tatctagctg gaggttgttt ctggggagtg gaggaatatt
     2701 tctcacgcgt tcccggggtg acggatgccg tttcaggcta tgcaaatggt agaggagaaa
     2761 caaccaagta cgaattgatt aaccaaacag gtcatgcaga aaccgtccat gtcacctatg
     2821 atgccaagca aatttctctc aaggaaatcc tgcttcacta tttccgcatt atcaatccaa
     2881 ccagcaaaaa taaacaagga aatgatgtgg ggacccagta ccgtactggt gtttattaca
     2941 cagatgacaa ggatttggaa gtgattaacc aagtctttga tgaggtggct aagaaatacg
     3001 atcaacctct agcagttgaa aaggaaaact tgaagaattt tgtggtggct gaggattacc
     3061 atcaagacta tctcaagaaa aatccaaatg gctactgcca tatcaatgtt aatcaggcgg
     3121 cctatcctgt cattgatgcc agcaaatatc caaaaccaag tgatgaggaa ttgaaaaaga
     3181 ccctgtcacc tgaggagtat gcagttaccc aggaaaatca aacagaacga gctttctcaa
     3241 accgttactg ggataaattt gaatccggta tctatgtgga tatagcaact ggggaacctc
     3301 tcttttcatc aaaagacaaa tttgagtctg gttgtggctg gcctagtttt acccaaccca
     3361 tcagtccaga tgttgtcacc tacaaggaag ataagtccta caatatgacg cgtatggaag
     3421 tgcggagccg agtaggagat tctcaccttg ggcatgtctt tacggatggt ccacaggaca
     3481 agggcggctt acgttactgt atcaatagcc tctctatccg ctttattccc aaagaccaaa
     3541 tggaagaaaa aggctacgct tatttactag attatgttga tt
//
LOCUS       DAVBCG010000154         3477 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVBCG010000154 DAVBCG010000000
VERSION     DAVBCG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.154"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            65..1305
                     /locus_tag="VQ495_001914"
     CDS             join(65..404,404..1305)
                     /locus_tag="VQ495_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2531832.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYLKKFKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
     gene            complement(1540..2148)
                     /locus_tag="VQ495_001915"
     CDS             complement(1540..2148)
                     /locus_tag="VQ495_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2531833.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2314..2484
                     /locus_tag="VQ495_001916"
                     /pseudo
     CDS             2314..2484
                     /locus_tag="VQ495_001916"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            2473..3084
                     /locus_tag="VQ495_001917"
     CDS             2473..3084
                     /locus_tag="VQ495_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2531834.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
BASE COUNT         1313 a          443 c          620 g         1101 t
ORIGIN      
        1 gcaaggacaa agcttcaaac agctttcaaa aagatttggt gtggatgttt ctggtctaaa
       61 gtacatggtg aaattaattg accgttatgg catagaaatc gttaaaaaag ggaagaatcg
      121 tcactattct tctaaattga aacaagaaat gatggataaa gccttactgg aaggttgttc
      181 acaaagaagt attagtcttg attatgccct cccaaaccaa ggaatgcttt cattttggcc
      241 ggcacaatac aagaaaaacg ggtatactat tgttgagaaa acaagaggga gaccagctaa
      301 aatgggacgt aaacggaaga agacttggga agaaatgaca gaactagaac gactccagga
      361 ggagaatgaa cgcttacgga ctgaggtggc ctacctaaaa aagttaaaag agttagagga
      421 aagggacgaa gccctagagc gagaaaggca gagacagtta gagaaatggt ttcaggagga
      481 tttcgactag attttcttct tgaaacagca cgtttagctc gctcgactta ctattatcag
      541 ttgaaacaac tggatggggt tgataaagat aaagagatta aaaccgaaat tcagggcatt
      601 gataatgaac ataaaggcaa ctatggctat cgtcgaatcc atttagaact aaggaatcgt
      661 ggttttgtgg tcaatcataa gaaagttcaa cggttgatga gaattcttgg tttaacggct
      721 cgaattcgcc gtaaacggaa gtattcttcc taccaaggag agattggcaa gaaagcagag
      781 aatctcattc aacgccagtt cgaagcatcg aggccaatgg aaaagtgcta tacagatgtg
      841 acagagtttg ccattccaaa tagcacacag aaactctatt tatcaccagt tttagatggt
      901 tttaacagtg aaatcattgc ttatcatctt tctacttcgc caaacttaga acaagtgaag
      961 agtatgttag aacaggcctt tacagagaaa tactatgaga atacgattct ccatagtgac
     1021 caaggctggc aataccaaca cgattcttat catcggttcc tagagagtaa gggaattcaa
     1081 gcatccatgt cacgcaaggg caacagccca gacaacggca tgatggaatc tttctttggc
     1141 attttaaaat ccgaaatgtt ttatggctat gagaagaact ttagatcttt agaaaacctt
     1201 gaacaagcta ttgtggacta cattgattac tacaacaaca aacgaattaa ggtaaaacta
     1261 aaaggactta gttctgtgca gtacagaact aaatcctttg gataaattat ttgtctaatt
     1321 ttttggggtc agtacagtat taaataccat gatcttatgc gttttataat aaaaaatctg
     1381 ttcttgtttt atttttagaa aatttactaa agactaaaaa tattatgaat aatttaaaaa
     1441 atagtcaggt tatatagttt tttgaaatta aaattatttt atcgttgaaa ttagttaaaa
     1501 atttttataa aatcaagatg caaggctatg agatgttctc tagtttaatt tttcatactt
     1561 cgagaatata aagttatata aaaatgcatt agcatatttt gttattgagg gaagtataaa
     1621 aatcattaaa gttatccaaa ctagtaattg attctgtaat ataaagaaga gtagaactgg
     1681 gataatttca ataattctaa taagaacggc aataaaaaaa tgtttatagg ctaacatgat
     1741 tgaattgtgt agagaatttt ttaaagtgtt attatatcta ccgattaaag gaattagaaa
     1801 tgttgttgct attaaatgaa gtatgatata tattactcca atatgtcgta agatagaaat
     1861 gatatatata ggtaatggta tgtagggtag gaagaacaaa ctgaataata agattaaaat
     1921 tggtaaatta gatattaatg ctgctcctaa atttttccaa taatctttaa aaaaactgtt
     1981 tgcgaagtct ctatagggat aggattcctc gtgtcgtaca atttttatta gagttgctat
     2041 tagggcactt tcacctgatc cgatagcaat aactgttact actgacgcta catagagtag
     2101 atttaacatt ataagatttg aaactttatc aataaatttt atctgcatct ttaactcctg
     2161 ctgtttttat acttataata tcacatcatt tgagtttcat caactttgaa tacattaaga
     2221 aagaatataa ctatgaggaa aggaaaaaat tattatgaat aaatccaaaa aatataaaga
     2281 aagaattcat tatgaatacg ataatcttac taaatcagag aaaaaattgc tgactatatt
     2341 ttagttagag atagtgaaat agtttatgaa acgtcttcag aaattgttag taaagtaaaa
     2401 gttggagagg gaactcttaa tcgattttgc aaaaagattg gatactctgg ttttcaggaa
     2461 ctaaaattaa aaatgacgaa agatatatta gagttagaat ctcaaaaaat gagtagtgac
     2521 acatttattg atgaaataaa aaataattat ttatctattg tggaaagtac aagaaaatta
     2581 attgatggta gacaaataga attagctata aaattaataa gagaagcaaa ccagatttta
     2641 atgattggtg tgggttctag tggaaatgcg gctagagaat ttgagagtag tttgttaaga
     2701 attggaataa taagtaagac ggtaattgat acccattttc aactcatgca tactgcgtta
     2761 ttgaaagata atgatttgat aatagcgttt agtttatctg gttccacaaa agaagttgag
     2821 gaaactttat taaacgcaaa aagaaaaaat gttaaaatta tttcaataac aaattattcg
     2881 tccagaaata tagcaaaatt atctgactgc gttcttttga catcaaaaaa agaatcttat
     2941 ttagagggtg gtagtttaat ggctaaggct tctcaactat tcataattga tgtaatatgc
     3001 actagactct ctttaataaa ttatgaagat actatatgta aaaaagaaga gattgcttct
     3061 ttgctgtcaa ataaagtgga ataaatcaat aatttttaaa ttttaataaa aattttttaa
     3121 aataaattaa aaaacgatcc taaaagggtc gtttttttgc taaagtttta aaaataaaca
     3181 ataattaaga ttatagtgcg ttgaatctat attaggacat aacaattctt aaatcattgt
     3241 tagaaattag tttgaatttt ccaatcaatt tgttcgattt ttacatcatt gcattatatc
     3301 acgtgactat taaccttgtt ttctcctaaa attgattttc ttcttttctt atcttgtcca
     3361 ctcgaaacaa gtattgtaag aatttgatta tttttgaaag tacttttaat atatttgaga
     3421 tagttaaaaa agatttgaaa ctaaattcca aattagaaaa agacttgaaa tgcttaa
//
LOCUS       DAVBCG010000155         3262 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVBCG010000155 DAVBCG010000000
VERSION     DAVBCG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.155"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQ495_001918"
     CDS             complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQ495_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2531835.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1022..2074)
                     /locus_tag="VQ495_001919"
     CDS             complement(1022..2074)
                     /locus_tag="VQ495_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2531836.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2071..2769)
                     /locus_tag="VQ495_001920"
     CDS             complement(2071..2769)
                     /locus_tag="VQ495_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2531837.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2936..>3262)
                     /locus_tag="VQ495_001921"
     CDS             complement(2936..>3262)
                     /locus_tag="VQ495_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2531838.1"
                     /translation="VKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT          912 a          735 c          626 g          989 t
ORIGIN      
        1 ttttttacga aaatctattg aatatgccat aaaaggatta taccacattg tgtactattt
       61 ttggttcatt ttactatatc actttggact atatcggtct agttatgtct aaacgattat
      121 agctggatac taagagaaat caaaaaagca ctaagggaga acgtcccaaa gtgcttaata
      181 tcaaggcctc aaagcctgtc ttattcaata aaatattaca acatcttgtt gtagaattca
      241 acgacaagtg cttcgttgat ttctgggttg atttcgtcgc gttctggcaa gcgagtcaat
      301 gaaccttcca atttttcagc gtcgaatgat acgaatgctg gacgtccaag agtagcttct
      361 actgcttcaa ggattgctgg aactttcaat gatttttcac gaactgagat cacttgacct
      421 ggagttacgc ggtatgatgg gatatcaacg cgtttcccgt caacaaggat gtgaccgtgg
      481 tttacgaatt gacgagcttg acgacgagta gtcgcgagac caagacggta aacaacgtta
      541 tccaaacgac gttccaaaag aagcataaag ttgaaaccta ggattccgcc tttgattttt
      601 gtagcttgta cgaacaagtt acggaattgt ttttcaccta caccgtaagt gaaacgaagt
      661 ttttgttttt cagccaattg caaaccgtat tctgacaatt tagaacggtt gtttggtccg
      721 tgttgtcctg gtacgtagtt acgacgtgcc aattctttac ctgtacctgt aagtgaaagg
      781 ccaaggcgac gagcttgttt ccaagatggt cctgtataac gtgacatatg aatgtcctcc
      841 tgatataaat aatatttcgg tggaaatagt cacttagaaa gccctgattc gtgcagatgc
      901 ccttcgccta aacagccaag gttacttgtc ataagacacc tgttgacgag cttcatgctt
      961 tcctgctgat atttcacaca aaatccattt taccatgaat agctggattt gtaaaggggt
     1021 tttaggcttt attttcacta ccagagagat tgagaacgag ggtaaaggta cttcctagac
     1081 cgtactggct gctgactgtg atttccccac ccaattgatg ggccaattca cgcgcaatcg
     1141 caagtcctaa tccatgacca cctgtcttca tgttacgcga agtttcgaca cgataaaggc
     1201 gtttgaaaat attttccaaa tcctctgggg caatgccctg cccttcatcg gtcacactga
     1261 ttgaaagctg gcccttctcc agcttagtca ccacttccag cttggttcct ggagcagaat
     1321 atttaaaagc gttatcgacc agattcacca agatacgaga aagcttagca taatctccct
     1381 caatccgggc agactctggg attacctgca agtggacatc tcttctctcc tgctcaatca
     1441 aaaactgaaa ttcactcatg cactcaatta agagcttgtc cagaaaaata ctgtctttac
     1501 tggtagtttc cacctgattt ctagctgtgt ttagggtcaa aaaattcaac tcctcaacca
     1561 gtttattgag cctctccgtc tggcgtccaa tggttgctag ataatgagct tgctccgact
     1621 ccttgataat cccatccaaa atcccttcta ccgtcgcttg gatcgaagtg ataggggtct
     1681 taatatcatg cgacaactgg gcaatcatca agcccttttc tcgttcgctt tcttccaagg
     1741 aatcaaagct tacctgcaaa tcatgggaca tctcattaaa agtttgccct aattgctgaa
     1801 attctacagg accttgaacc tccaaatttg aaggaaaatc cttggccgct acccgcttgg
     1861 catgctcctt gagtttgccc aacgacgtaa agactggcaa taggagaaag agactaatcc
     1921 cagcaccgac aaggctggca acgatggtca tcccaagcaa aaagtaaatc tcgcctttcg
     1981 caatcagcat tttttgaaca gcccaaaaaa caaccaaaat ggttaagagg gttgaaataa
     2041 tatatccaac caaaatataa ctttttagtt tcatgtttgt cctctcggtt tctctatctt
     2101 atatcccaac ccccaaactg tcttaatagt gggcgtttgg tcactactat attttgccag
     2161 ctcctgtcga agagcatgga tatgcacatt caaggtattg gtgtcatcca cgtagtcttc
     2221 tttccagatc ttttcataga ggtctgtctt ggagaaaact cgctctggat tgctagctaa
     2281 aatccacagc aattcaaatg atttaacagt taaatccagc atttcttctc ctatttgaac
     2341 ttcatgacta ctatgattca tttttagatt gccaagggaa atcagctctg tttcgccccc
     2401 acgatgaagg cggcgcaaaa tattgtggac acgcaaaacc agctcacgtg ggctaaaagg
     2461 cttagcaata aaatcatctg ctcccaagct caggccgtaa atcttgtcct gttcactggt
     2521 cttagcagta ataaatagga aaggctgctc tggtgataag tattgaacct cactgattaa
     2581 atcataacca tccatccgag gcatcataac atctgtgata atcaaatcaa tcggtttttt
     2641 cttgaacagc tctagcgctt ccagtccatc atgggctacc aagacctgat aacctgcctg
     2701 aattaagtat ctctgatgaa tatctgtgat ttctacctcg tcgtcaacga gtaaaattgt
     2761 ctttcccatc tgtctctcct ttgataaaaa cagtgctata ctgctttaag tataacacta
     2821 tttttattgt tttacgatga ttttaatctg ctattgaaag aaagtcctga ttgctgattg
     2881 agttgttaaa ctaatcttag tcagcccact atccatcatt tatttcctta gctgattagt
     2941 tttcttcttt tttgtttttc aaacctagac caccgagtaa acctgcaagc gcaagcccaa
     3001 ggaaaccaat acttgccatt gatgtttgag tctcaccagt atttggtagc atagctttat
     3061 cctctgacat catcgtatca gacatcttgt tagcagaagc agccatgttt tcacctgcca
     3121 tcgtgttggt agaacttgtc atggtgtcag caggcatgct atctgtagta cctgtagcat
     3181 gattgtgatt catcggagtc acgccagaac cagagttaga aggtgataat gaaccatttg
     3241 ctgtgtctga tgtttcttta ac
//
LOCUS       DAVBCG010000156         3242 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVBCG010000156 DAVBCG010000000
VERSION     DAVBCG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.156"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            200..2359
                     /gene="pfbA"
                     /locus_tag="VQ495_001922"
     CDS             200..2359
                     /gene="pfbA"
                     /locus_tag="VQ495_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2531839.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            2343..2639
                     /locus_tag="VQ495_001923"
     CDS             2343..2639
                     /locus_tag="VQ495_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531840.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            2881..3189
                     /locus_tag="VQ495_001924"
                     /pseudo
     CDS             2881..3189
                     /locus_tag="VQ495_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
BASE COUNT         1266 a          400 c          608 g          968 t
ORIGIN      
        1 ttcagtctac tatagaactg accaagtcag taacctagac ttagggcaag gcggcactga
       61 cctagtttga agagatttcc gaagagtata aattttaata ttttcttgtg ttattccttg
      121 acaattcaat ttggaaaata tatgataaag ataatgacag cggtgtcatt ctatctattt
      181 taagaaaagt aataatcaat tgttaaaaat agtaaaaaaa ttggaggttc tgatgaaata
      241 ttttgttcct aatgaggtat tcagtattcg taaattaaag gtggggactt gctcggtact
      301 attggcaatt tcaattttgg gaagccaagg tattttatcg gatgaagttg ttactagttc
      361 ttcaccgatg gctacaaaag agtcttctaa tgcaatcact aatgatttag ataattcacc
      421 aactgttaat caaaatcgtt ctgctgaaat gattgcctct aattcaacca ctaatggttt
      481 agataattcg ttaagtgtta atagtatcag ctctaatggt actattcgtt ccaattcaca
      541 attagacaac agaacagttg aatctacagt aatatctact aatgaaaata agagttataa
      601 ggaagatgtt ataagtgaca gaattatcaa aaaagaattt gaagatactg ctttaagtgt
      661 aaaagattat ggtgcggtag gtgatgggat tcatgatgat cgacaagcaa ttcaagatgc
      721 aatagatgct gcagctcaag ggctaggtgg aggaaatgta tattttcctg aaggaactta
      781 tttagtaaaa gaaattgttt ttttaaaaag tcatacacac ttagaattga atgagaaagc
      841 tacaattcta aatggtataa atattaagaa tcacccttcc attgttttta tgacaggttt
      901 atttacggat gatggtgcgc aagtagaatg gggcccaaca gaagatatta gttattctgg
      961 tggtacgatt gatatgaacg gtgctttgaa tgaagaagga actaaagcaa aaaatctacc
     1021 acttataaat tcttcaggtg catttgctat tgggaattca aataacgtaa ctataaaaaa
     1081 tgtaacattc aaggatagtt atcaagggca tgctattcaa attgcaggtt cgaaaaatgt
     1141 attagttgat aattctcgtt ttcttgggca agccttaccc aaaacgatga aggatgggca
     1201 aatcataagt aaggagagca ttcagattga accattaact agaaaaggtt ttccttatgc
     1261 cttgaatgat gatgggaaaa aatctgaaaa tgtgactatt caaaattcct attttggcaa
     1321 aagtgataaa tctggggaat tagtaacagc aattggcaca cactatcaaa cattgtcgac
     1381 acagaacccc tctaatatta aaattttaaa taatcatttt gataacatga tgtatgcagg
     1441 tgtacgtttt acaggattca ctgatgtatt aatcaaagga aatcgctttg ataagaaagt
     1501 taaaggagag agtgtacatt atcgagaaag cggagcagct ttagtaaatg cttatagcta
     1561 taaaaacact aaagacctat tagatttaaa taaacaggtg gttatcgccg aaaatatatt
     1621 taatattgcc gatcctaaaa caaaagcgat acgagttgca aaagatagtg cagaatattt
     1681 aggaaaagta tcagatatta ctgtaacaaa aaatgtaatt aataataatt ctaaggaaac
     1741 agaacaacca aatattgaat tattacgagt tagtgataat ttagtagtct cagagaatag
     1801 tatattcggt ggtaaagaag gaattgttat tgaggattca aagggtaaaa taaccgtttt
     1861 aaataaccaa ttttataatt tatccggtaa gtatatatca ttcatcaaat ctaatgcaaa
     1921 tgggaaagaa tctgttatac gtgatagcga tggtaatttc aatattgtaa cggagaatgg
     1981 gctttacaaa attgtaacaa ataatttaag tgataaaaac gaaaaagaaa aaaacaaaga
     2041 ggaaaaacaa tctaattcaa ataatgtaat tgatagtaac cagaagaacg gagagtttaa
     2101 ctcaagtaaa gataatagac aaatgaatga caagatcgac aataaacaag ataataagac
     2161 agaagaagta aactataaaa tagttggaga tggcagagaa actgaaaatc atattaataa
     2221 atctaaagaa atagtagatg taaaacaaaa attaccaaag actggttcga acaagattat
     2281 ggaactattc ttaacagtga caggaattgg tttacttttg acactaaaag ggttgaagta
     2341 ttatggtaaa gataaataaa atttgttcga tacaaggaag ttccgtagaa aatgaggata
     2401 ttgtaggttc acaaaatcag tatttttgga ttattggtgg agctacagat ttatataatt
     2461 ctaaagagga gataggttat tcagtctcag aagttgtaca tattttatca gaaagtttgt
     2521 cggttaattg taaagaatca aaaactctta aacaaatatt tgaaactgct ttactcgagg
     2581 ttaaagatga aatcggttta aactcatatg aattaacaga gtatagtaaa atgaaataat
     2641 aataggacaa atcaatcagg acagtcaaat cgatttctaa caatgtttta gaagtagagg
     2701 tgtactattc tagtttcaat atactatatt atcgcttacc aatatttgct aaactttcag
     2761 aaaaaaagtt ggagtatatg atgctaggtg actgcgtcat gttagtaaac gaaatggaaa
     2821 taactgatca tagagtagat aacttatttg aaaaagggaa aaatgagata aaagattcaa
     2881 gtagtagatt tagcaatagg acatataaaa gctttagaaa aagtatctga aaaaacagat
     2941 gtttatatct ataacctagg ctcgggagaa ggcactagtg tattacaact tgtaaataca
     3001 tttgaaagtg ttaataagat ccctattcct tataaaatag ttccaagacg ttcaggagac
     3061 gttgcaactt attatgcaaa tgcagacaaa gcatataagg aattaaattg gaggacaaca
     3121 aaatcgattg aagacatgtg tagagataca tggaattggc aatcaaaaaa tcccaatggc
     3181 tataattgat gatactgtct atatgagtgc tgtattttgg aaaaattact taacctttta
     3241 at
//
LOCUS       DAVBCG010000157         3240 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVBCG010000157 DAVBCG010000000
VERSION     DAVBCG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.157"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            116..2245
                     /locus_tag="VQ495_001925"
     CDS             116..2245
                     /locus_tag="VQ495_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2531841.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            2232..2681
                     /locus_tag="VQ495_001926"
     CDS             2232..2681
                     /locus_tag="VQ495_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2531842.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            2740..3012
                     /locus_tag="VQ495_001927"
     CDS             2740..3012
                     /locus_tag="VQ495_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2531843.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT          946 a          629 c          737 g          928 t
ORIGIN      
        1 aacctcaaaa cagtattttg agcagcctgc ggctagcttc ctagtttgct ttttgatttt
       61 cattgagtat gaaaaggatt ggagaaagcc ttccagcttg tggtataata gatttatgga
      121 taaaaaatat gaaaaaatct ctcaggattt gggagtgatg ttaaagcaaa ttgataccgt
      181 tctaagtttg acagctgaag gggcgactat tccctttatc gcgcgttatc gcaaggacat
      241 gactggtagt ctggatgagg tggcgattaa ggctattatt gatttggata aaagtctgac
      301 aaatctcaat gaccgtaagg aagctgtctt agctaagatt caagaacaag gtaagttgac
      361 caaggaattg gaagaagcta tcttagttgc cgaaaaatta gcagacgttg aagaactcta
      421 tcttccttat aaggaaaagc gtcgtaccaa ggcaaccatt gcccgtgaag ctggactctt
      481 tcctcttgct cgtttgattt tgcagaatat agttgactta gagaaagaag ctgaaaagtt
      541 cgtctgtgaa ggatttgcga ctggcaagga agccttgacc ggtgcagttg atattttggt
      601 cgaagcctta tcggaagatg tgaccttgcg ttctatgact tatcaggaag tgctgagaca
      661 ctctaaactt acttctcaag ccaaggatga aagtcttgat gaaaagcagg tttttcagat
      721 ttattatgat ttttcagaga cagttggaac tatgcaaggc tatcgtacct tggctctcaa
      781 tcgtggggag aaacttggtg tcttgaagat cggttttgaa catgcgacgg accgtattct
      841 tgccttcttt gctactcgtt tcaaggtgaa aaatgcttat attgatgaag ttgttcagca
      901 atccgttaag aaaaaggtct tgcctgctat cgagcgtcgt attcggacag aattaactga
      961 gaaagctgaa gaaggagcta tccaactttt ttctgacaat ctgcgcaatc tcctcttggt
     1021 tgctccactg aaagggcgcg tggttcttgg atttgaccca gcctttcgta caggtgccaa
     1081 gttagctgtc gtggatgcaa caggaaaaat gctgacaact caggttattt atcctgttaa
     1141 accagcatca gctcgtcaaa tcgaagaagc caagaaagat ttagcagatt taattggtca
     1201 atacggtgta gagattattg ccattggaaa tggaacggcc agtcgtgaaa gtgaagcttt
     1261 tgtagcggaa gttctgaaag atttccctga agtcagctat gttatcgtta atgaaagtgg
     1321 tgcttctgtc tattctgcca gcgaacttgc tcgtcaggag tttccagact tgaccgttga
     1381 aaaacgctct gccatttcta tcgcccgtcg tttgcaagat cctcttgcgg aattggtcaa
     1441 aatcgatcct aagtcaattg gtgtcggtca ataccaacac gatgtcagtc agaagaaact
     1501 atctgagagt ctggactttg ttgtcgatac agtggttaac caagttggtg tcaatgtcaa
     1561 tacagctagc ccagctcttc tttcacacgt agctggactc aacaaaacta tctctgaaaa
     1621 tattgtcaaa taccgcgagg aagaaggaaa aatcacttca cgcgcccaaa tcaagaaagt
     1681 tcctcgtctg ggagccaagg cctttgagca ggctgctggt ttccttcgta tccctgaaag
     1741 tagcaatatc cttgataata caggagttca cccagagaat tacactgcag ttaaggaact
     1801 cttcaaacgt ttagatatta aagacctaaa cgaagaagcc caaagcaaac tcaagtccct
     1861 ttcagtcaag gaaatggctc aagagctaga cctcggtcca gaaaccctta aagatatcat
     1921 tgcagatctt ctcaaaccag gtcgagattt ccgtgattcc tttgacgcac ctgtgcttcg
     1981 ccaagatgtc ctagatatca aagacctagt ggtaggtcag aagctagaag gtgttgtgcg
     2041 taacgttgtt gacttcggtg ccttcgttga tatcgggatt cacgaggacg gcttgattca
     2101 tatttctcat atgagtcgca agtttatcaa acaccctagt caagtggtgt cagttggaga
     2161 tttggtgacc gtttgggtca agaaaattga tactgaacgt gaaaaagtta atctgtcgct
     2221 cctcgctcca aatgaaactg actgattatg tcaagcaggt ttcactagaa gacttcggga
     2281 gagcttttat ccatcatgtc cagtggaata ggcgtctacg ttcgacaggt gggcgatttt
     2341 tccccaaaga tgggcatctg gattttaatc ccaaggttta tcagaaactg ggtatggagg
     2401 tttttaggaa aattgtccga catgaactct gtcattatca cctttatttc caagggaagg
     2461 gctatcaaca caaggatcgg gattttaagg aactgttgaa agcagtggat ggattaaggt
     2521 ttgtaccatc cttgcccaat agtaactcta aaccactcaa gctttatcgt tgtcaatcct
     2581 gccagcaaag gtatcagcgc aagcgtagga ttgataccaa acgctatcgc tgtggacttt
     2641 gtcgaggtaa attgcttctg ataaatcagc ctgaggactg atgaaagccg agcctccccg
     2701 tgatatacta tgtctaacct agcagaaaga ggaaatatca tgaacagtaa attttataaa
     2761 atgagacgca atcgcatgat ttcaggagtt ctggcaggtc tgtcagacaa gtggaatttt
     2821 gatgttaccc tagttcgttt cttgtttgcc atttttacag ttgcaaactt tggacttggc
     2881 gtgattatct atatcatcct agcttctatc atgcctacca aggaagaaat cgaagcagaa
     2941 atgtacggaa caggcccacg taaacgcaaa gaagcccaag ccattgacga caatgaaggc
     3001 tggttttggt gaggttctac agaagatttg gaataagcaa aaagctttaa atcaatagtc
     3061 taaactgctt gatttaaagc ttttttactt ttatactcaa taaaaatcaa agtgcaaact
     3121 aggaagccag ccacaggctg taatttagtc ggcaatgtga agttgacata gtttgcactt
     3181 tgatttttga atagtattaa taatatttcg ccagcttcat ccacaaacta tagtggattg
//
LOCUS       DAVBCG010000158         3231 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVBCG010000158 DAVBCG010000000
VERSION     DAVBCG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.158"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            135..425
                     /locus_tag="VQ495_001928"
     CDS             135..425
                     /locus_tag="VQ495_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531844.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKNAKNCVRLSQLSLNKR"
     gene            449..1165
                     /locus_tag="VQ495_001929"
     CDS             449..1165
                     /locus_tag="VQ495_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2531845.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(1294..2109)
                     /locus_tag="VQ495_001930"
     CDS             complement(1294..2109)
                     /locus_tag="VQ495_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2531846.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2106..3011)
                     /locus_tag="VQ495_001931"
     CDS             complement(2106..3011)
                     /locus_tag="VQ495_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2531847.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
BASE COUNT         1082 a          543 c          675 g          931 t
ORIGIN      
        1 tataccatat tttgtttcgc aggaagtcta atgctaaaat cttatcagtg agaagatttc
       61 ttaaagttta ttctttgtag tttcatctgt attagtacat aatttaactt agagaaaata
      121 ggaggactaa tcgaatggaa ttaaaagatt ttacagaaaa agaacaggaa atgattaaga
      181 aaaggcttac aatgtctaat attagtgaca aagaaactac tgagaagatt ctggcgctag
      241 tcccacaaga cttgattaag cgaatcccgt tttttgtcag aaaacatgct acaacacgta
      301 cgattaaacg tatttcaatt gaataccctg aactctacgc tgtagctcaa acaagtggtg
      361 agattccaga aaaaaacgcg aagaattgcg tcagattatc acaactatct ttgaacaaaa
      421 gatgaataag catagtatta agtagagaat gaaaaaatat tttattggcg gtttgggaag
      481 caatgcctat catagcaagg attttcttca agaactagat tcgcaggtct attttctaaa
      541 tccatatgaa aagcatcttc gagatgaaac agaattgaaa tcatggttta aaaatgagat
      601 tgtagaggaa gaatctatct gtctgatagg tcattctatt ggaggcgatt tagctcgtta
      661 tttcgcatcg gaatttgaag aagtaaagaa actgattctt ttggatggtg gctatctaga
      721 tttagataag attttacctt tggatacaga gttagaggaa actaaaaatt atatcaaatc
      781 tcaaattatt tcggacttag atgttcttac ttctaaagaa aaatctgaag caaagcattg
      841 gtcagaaaat atggagaaag ctgtaagaca gtcctatcac tggaatgttg agtataatag
      901 atatgagttg gctataaatt atgaaaatat agaagcgata ctccgcctac ggagaaaaat
      961 acaagctttt aagagagaag tgggagatac cttgtttatt agtcctcgct atcctaatga
     1021 agctacatgg agagaggaag ccctaaaaga attgccagac tattttgata ctattttttt
     1081 agaaaacttt ggccatgagc tttatactca agcacctaaa gaaatcgcta gtctaatgaa
     1141 tgagtggctc gcttattttc tatgagctac ttgtattgct ggaaatggta taagatgaaa
     1201 atctacaagt cactaatttt catataacaa aaaacgaaca ggctcttttt ctgttattca
     1261 gaagagaact tgttcgtttt tatcttgatt ttcttaaaaa gctctatata catagggatt
     1321 ttctggttta tcgactttgg taaagctgtc gatttccaag gttggaaggt tttgtttgag
     1381 ttctttatgg tagtgattga ccagttttcc tttggctgtt atccaagtgt tgttttcata
     1441 ctgccgggta ttgcccttgg tcagcaatcc atagacacca gaatctgcga tacagtggat
     1501 aatgccgaat cggaacagaa attgactatt ggcatgactg gggtcgttat agacaaagcc
     1561 tgtaaactgg attgtcttgc cctcaaactc atctggatag tcgtagatag cctccatgac
     1621 ttccatatag ttttcattag tgatctgaat actatcttgg gataagtatt tatccgccgc
     1681 cgttcgcatt tccttttcat aggctgattt tgaaaaataa gaactggtat ctggtttcaa
     1741 atattggctt gtcgtccctt cgcttgtctg aatggctaga tctgttcctt ccgatagggg
     1801 gaaatgataa cctttagcag aaacggtctg agaatccaag ctaacagttg ggaaagttaa
     1861 gccgatgaca agtggaatag ccagaagaga aatactcgtc atcttggcca atcggctgtt
     1921 cagatgactg tgggttttga cttgcttcat ccagatatac aattgaacaa ttgccaagat
     1981 aaaggaaagc accatggaga tataggccag ataggaatag tgcatgttga tgtactggtt
     2041 tagtttgccc gacagatgga ggtaaatagt cagttcaaaa tagccagcta aaactaaaaa
     2101 tcgaatcata ggatgactcc aatcaagaga gaatagacta agacgacaag agttacaatc
     2161 gttatgaagt gactgataaa tcgtgctttc aagtaatttt tcatcatgag aatatttttg
     2221 atatccagca ttggaccaat gacgagaaag gccagaactg gtgccaaacc gaaactcgag
     2281 agaagagaag cacctataaa ggcatccgcc tcactacaga gcgaaagaag aaaggccaaa
     2341 atcatcaaga gcaggatggc aaaaagagga gtcgcactga tagaggtcag aatccgagtc
     2401 ggaacgtaga cctgtattat agaagcaaag aggcagccaa ataccaaata acgccccgta
     2461 tcaaaaaatt catcaatggc ctgcacaaag acttgaaaaa ctttttttgc agaactcaag
     2521 taagaaaaat catgctcatg acaagccaga cgattttctt tctgaatcgg ttcttgccag
     2581 aaaaatccta gaaaaattcc tagtattaca gccacaagaa tggaacccag agctcgtaat
     2641 agggcgacat ggaaggagtt gccaaaggca gaataggtcg caaaaagaac aatgggattg
     2701 ataacaggtg ctgtcacaag aaaaggaacg gctgtgtaac tgggaacctt tttttccaga
     2761 aaacgattga tgatggggac gattccacat tcacaagaag ggaaaagcat accgacaaag
     2821 gtcccaaaaa agattctccc ccaacgattt cgagggagaa aatgataaac cttgtcaggt
     2881 gtgatataaa cttcaatcag ccctgagaca atgcttccta ttagaacgaa gggaagggct
     2941 tcaatgataa tggagagaaa aatggctcca gtttgaagta cactagatgg gagttgttga
     3001 aaagcagtca tctagttttt cttttctgct ttggcttttt tctctttgtc atcagggaaa
     3061 gtaacttgtt tcaaatcagg gagtttagca aactcttcaa acatcttgtc caagtcgtct
     3121 cgttttgtaa attttacagc cataggtgta cctcgattct aaagtctacc tctattatac
     3181 tattattttg cggaaaaggg agttttttaa tttgttttcg atagttgata c
//
LOCUS       DAVBCG010000159         3219 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVBCG010000159 DAVBCG010000000
VERSION     DAVBCG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.159"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(19..726)
                     /locus_tag="VQ495_001932"
     CDS             complement(19..726)
                     /locus_tag="VQ495_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2531848.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLVLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(779..2197)
                     /locus_tag="VQ495_001933"
     CDS             complement(779..2197)
                     /locus_tag="VQ495_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2531849.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLTMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNDENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            2387..3199
                     /locus_tag="VQ495_001934"
     CDS             2387..3199
                     /locus_tag="VQ495_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2531850.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
BASE COUNT         1097 a          700 c          579 g          843 t
ORIGIN      
        1 gttcctacat aaaaactttt acacagaaat tcctaatgtc cgactcataa tcaccacaag
       61 agccaacaaa cagaaagcaa tcccattaac aatcatgtga agcaagatcg acatttccaa
      121 acgttgggtc ttgtaggccg tccaagatag aactgtcgac atacctccat aaatcaataa
      181 agaaggtaaa ttacttggtt gatgcaataa agcaaacaca atcgtaccga ctacaaatcc
      241 caagttctcc ttgcctcgga aaatcttttt aggaacaatc ccacggcaca agatttcctc
      301 acaaattgga gcaagcaagg ctagcaagaa gaagctagaa atcaacgaac tattctgaac
      361 catatcgtta atctgagact ggttagctgt tgtcgtctca tttgacagtt gcaacaagat
      421 agaaccaagt atatttgaac agataatgac taaataactc aagaccaaac gtgccaaatc
      481 tttagctcta aaaaaagaaa aattaaaact agctaacttg gttttacgag ctcccataat
      541 aaatagagcc agaaccacaa ttgaaaggcc agcaactatc agccctgact gtagcagggc
      601 tacttctttt aaagtcaaaa tagaggtaac cactagggga acctgatata aaaggacggc
      661 aagtaaaaag accaaaatcc agcctgcacg attcaacaat tctttccaca tatttttctc
      721 tttcatcacc tatctccttt gtattaaaaa aagggggaaa tccccctggg tcattctatt
      781 ataaggccat gctgatgtag ttaaggataa acaaggcatc caaaatccaa atcatggcat
      841 gaacatcttt aacttgacct ttgacaagct tagtcaaagt gtaagtcaag aaaccaactg
      901 caatcccttg agtgatagag tagctgaatc ccataaagat agatgtgaag aaggcaggaa
      961 ctgcttcaga catatcatcc caatggatat ttttcaagct accaagcatc ataatcccaa
     1021 cgataatcaa gattggagct gtagccgctg ttggtacgat cgctagaagt gggctaaaga
     1081 agcttgaaat cgcaaaacag atagctacaa ccaaggctgt caaaccagta cgtccacctg
     1141 caccgatacc agcagcagac tcaacataag tcgttacgtt tgaagtacct gcaatggcac
     1201 cgactgtcgt tccaatcaaa tcagagtaaa gagccttatc caatttggct gattggtgat
     1261 tttcatcatt tgtcgctaca ataccaactt tttcacctgt accgatcaag gtaccaattg
     1321 tgtcaaaaat atctgtcaat gagaaggcaa gaatggccat cagagtttca ggcaagcgag
     1381 ctgtatctga aaccaaagct cccaaacctt ctgaaccaag agctgcacca aagattgtct
     1441 tcaaatcttc aaaagctgca ccaacatggt tattagcaaa atcgatacta gacaaatcaa
     1501 ccaaaccaac tgcaatagca agaacagttg ttgtcaagat agagagaata attccccctt
     1561 taatcccttt gatgacaaag aagatagtaa tggcaagtcc tgcaagagcc actaaaacag
     1621 ctggattatt aaagctgacc aatcctggaa ctgctgaaga gtttgctgca atcgttgctt
     1681 gagctttgtc agccccttct cctacaacag tatagttgcc tggatcaatc gtgaatttca
     1741 aaagtccagc attcttaatc cctacatagg caaggaagac accgatacca gctgaaatag
     1801 ctgagcgaag agcattggga atcgattcaa tgatcatttt acgaacattt gtcaaggtaa
     1861 taatcaatga aataatccca cagatgaaga ccatagctag ggcttcttgc caagaataac
     1921 caagtccaaa tacgactgta aaggtaaaga aggcattgag tcccatacct ggtgcttgag
     1981 cgtatggcaa gttagcatag aaggccatcg tcaaggtacc agcaactgca ccgataatcg
     2041 ttgcaagaaa cacaccctga gcaggcattc ctgtttgtga aagtatttgt gggtttacaa
     2101 agagaatata gctcattgca aagaaagttg ttaaaccagc gagaacctct gtacgaacgt
     2161 ctgtaccgtt ctcttttagt ttaaataatt tgtccatcat caatctcctt ttaattttta
     2221 cgaacaataa tagaattata tcatttatca ttcacttttt caatgtcttt gtttgatttg
     2281 tttaagaaat taatccaatt tcattttttg tttggaatct gcttttctgc ctgctttctg
     2341 ttataataga agacatctta tctgaaaaca cactagaaag gtccatatga cgaaaaaaat
     2401 tattgcagtt gacctagatg gaaccctgct caactcagac agtcaaattt ctgactttac
     2461 caaaaaaacc atcaaaaaag ttgctgaaaa aggccatcag gttattatta cgacaggtcg
     2521 cccttaccgt atgtcaaaag atttttaccg tgaactgggc ttagacactc ctatgattaa
     2581 cttcaacgga tcccttactc atttaccaga ccaagtttgg gattttgaaa agtgtttgac
     2641 tgtagacaaa aaatatctgc tagatatggt tcaacgttca gaggacattc aagccgattt
     2701 tatcgctgga gaatatcgta aaaaattcta cattacaaat cccaatgaag aaattgccaa
     2761 tcccaaacta tttggtgtag aagctttcca gcctgaagat caattccagc ctgaattggt
     2821 gaccaaggac cctaactgta tcctcttgca gaccagagcc agtgacaaat atgccttggc
     2881 aaaagaaatg aacgccttct accagcatca actttctatc aatacctggg gaggtccgct
     2941 caatatcctt gaatgtaccc caaaaggtgt caacaaggcc tttgctttgg actacttgct
     3001 caagataatg aatcgtgaca aaaaagattt gattgccttt ggagatgaac acaatgatac
     3061 cgaaatgctc gcttttgctg ggaagggtta tgccatgaaa aatgccaatc cagagctact
     3121 cccttatgca gatgagcaaa tttcccttac caacgaccaa gatggggttg ccaaaaccct
     3181 acaagactta ttcttataac ctatactgat actcaatga
//
LOCUS       DAVBCG010000160         3192 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVBCG010000160 DAVBCG010000000
VERSION     DAVBCG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.160"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(381..914)
                     /locus_tag="VQ495_001935"
     CDS             complement(381..914)
                     /locus_tag="VQ495_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2531851.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ495_001936"
     CDS             complement(1003..1779)
                     /gene="recX"
                     /locus_tag="VQ495_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2531852.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYKLKQRLTQVLARKGYDFSDIASALREYL"
     gene            1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ495_001937"
     CDS             1817..3172
                     /gene="rlmD"
                     /locus_tag="VQ495_001937"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2531853.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1028 a          562 c          581 g         1021 t
ORIGIN      
        1 atttcatttt actatactgt tacgaaatta gatatataaa tgaaaacttg cctcaaggga
       61 ggttttttgc tagtagaaaa gcgatagaca gaaattgttg gactcttaaa aaaatgaagt
      121 tttgctcaaa agagcttctt aaattaaaac gagcaattat cagttcaaat ctgtttcaaa
      181 agaagaacgg ataaattaat cgatgctttg tttttgagtt aggaagttaa gcatgccaaa
      241 ctgagtcgta ggaataaatt tctttataga aatgtgttgt aactctgcta taatggattt
      301 attccttttt gtgtttacac aatttatttt atactaccaa aaaaacaagg tcaggatttt
      361 gttcctgacc tttgacaact ttaccgattc tttagttcta catagcgctt gtaccaaatg
      421 tttacatagg cttctgagaa aggaccacgt ccattgttaa tccaatcaac aagaattttg
      481 acatgttctt ttaaaatata gtccaagtca tcagaataat tcattttgcg tttgtgacgc
      541 tcgtactctt caacgtccaa gagacgtttt tccccatctg taaaaatttt aacatccaaa
      601 tcgtaatcaa tatacttcag tgcttcttca tccagatagt aggggctagc catattgcaa
      661 tagtaagaag ttccattatc acgaatcatg gcaatgatat taaaccaata tttcttgtga
      721 aagtaaacaa tagccggttc tcgagtgacc caacgacgac catcactttc ggtaacaagt
      781 gtatgatcgt tgacaccaat aatggcgttt tctgttgttt ttagtaccat ggtgtcccgc
      841 caagttcggt ggagactccc atcatgctta taactttgaa ttgtaataaa gtcgccttct
      901 tttggaagct tcataactaa ccaactttct acaatttata agtttatcat ttactattgt
      961 accataaaat tacctaaaat ctgtgaattt cacttggaaa tattaaagat attctctaag
     1021 agcgcttgct atatccgaaa aatcgtagcc ctttcgtgct aaaacttgag ttaaacgctg
     1081 cttcagtttg tatccttcat actttcgggc atacttagta tattgcttat caagttcctt
     1141 gaagatgagt tcctgagtcg tttcttcatc aacttgacta tccaattcgt caaaggcaat
     1201 tttagcatca aaataagaga agcccttgtt agtcaagttc tggataatct tatcttgcag
     1261 ggcacgagct ggaagttttc cctcatattt tttcaatagt ttattggcta cacgttgagc
     1321 aacttccgaa aaatcaaaat cattcaagat ttcttctata gtagattttg aaattccttt
     1381 ttgtgctaat ttctgagtca gtacataagg tcccttgtct cctgaaagtt gattggcatt
     1441 gatgatagca taagcgtact gaccatcatt aatccactta tcttctttaa gattagcaat
     1501 aacttgagaa acgatgtttt tatcaatatc gtattttttc agatattctc tgacttcttt
     1561 ttcagtgcgt gctttaaagg ataagtggta gagggccaga ttcttaccat aagaaaattg
     1621 agcaaagtct tgaatctctt tcaattcctc ttcgcttatc accttatctc tcgataacat
     1681 aaaacgaaca attgtatctt cggtgatata gcatttgtcg ccattatcaa gctccatcag
     1741 atagagtctt tttttctttt caagttttgt gattttcata gctctattat aactcaaaat
     1801 gtgataagat aggggtatga atctgaaagt gaaacaaaaa ataccattaa aaatcaagcg
     1861 catgggaatt aacggtgagg gaatcggctt ttaccaaaaa acattagtct ttgtaccagg
     1921 agctctcaaa ggcgaagata tctattgtca gattacttct attagacgca actttgttga
     1981 agcaaaatta ctgaaggtca acaagaagtc taaatttcga attgtgccat cttgtactat
     2041 ttataatgaa tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt
     2101 caagacggac ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta
     2161 tgaaattcgt ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt
     2221 tcagactcga aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta
     2281 tttagtagag ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg
     2341 cttagcagaa ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg
     2401 tgtccgtact attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt
     2461 tacaaaccgc cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga
     2521 agttgtgaca gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa
     2581 gacagagatt atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc
     2641 actatcccct cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga
     2701 agcagtaaaa gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt
     2761 tggaacgatt ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat
     2821 tccagaagct attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca
     2881 ttatgaagct ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc
     2941 agatgctttg attgttgacc caccacgtac aggtctggat gataagttat tagatactat
     3001 tcttacttat gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg
     3061 tgatttggta cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt
     3121 cccacataca gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaaggtg
     3181 ttgacaaaac at
//
LOCUS       DAVBCG010000161         3046 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVBCG010000161 DAVBCG010000000
VERSION     DAVBCG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.161"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            89..847
                     /locus_tag="VQ495_001938"
                     /pseudo
     CDS             89..847
                     /locus_tag="VQ495_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            887..1651
                     /locus_tag="VQ495_001939"
     CDS             887..1651
                     /locus_tag="VQ495_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2531854.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1664..2341
                     /locus_tag="VQ495_001940"
     CDS             1664..2341
                     /locus_tag="VQ495_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2531855.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2351..3010
                     /locus_tag="VQ495_001941"
     CDS             2351..3010
                     /locus_tag="VQ495_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2531856.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
BASE COUNT          843 a          732 c          578 g          893 t
ORIGIN      
        1 tatagtgaat tttttcagat atggtttgac aagaacattc tttcattgta gaatcgtgtt
       61 ataaaatata acacaaagga gatctctcat ggctttagta gaatttaaaa acgtcgaaaa
      121 atattacgga gactaccacg cactccgcaa catcaatctc cgttttgaaa aaggacaagt
      181 tgttgtcctg cttggacctt ctggctctgg gaagtccact cttatccgta cgatcaatgg
      241 tttagaggct gttgacaaag gaagtctcct agtcaatggg caccaagttg ctggtgccag
      301 ccagaaagat ttggtacctc ttcgcaagga agtcggcatg gtttttcaac attttaacct
      361 ttatccacac aaaacggtgt tagaaaacgt gacacttgcg cccattaaag ttctaggaat
      421 tgataaaaaa gaagctgaaa aaaccgccca aaaatatctg gaatttgtaa atatgtgaga
      481 caagaaagat tcctatcccg ccatgctatc tggtggacaa aaacagcgga tcgccatcgc
      541 tcgtggtctt gctatgcatc cggaactcct cctctttgat gaaccaacat ctgctcttga
      601 tcctgagact atcggagatg ttctagcagt tatgcagaaa ctggcgcatg atgggatgaa
      661 catgatcatc gttacccacg aaatgggctt tgctcgagag gttgcggacc gcattatctt
      721 tatggccgac ggagaagttt tagtagatac gacagatgtc gataactttt ttgacaatcc
      781 aagcgaacct cgtgcccaac aattcctcag caaaattatc aaccacgaaa gtgacaaagt
      841 caaataagga ggcgcctatg aaaaaaaatt ctttttatca gcattattga ttagcctttt
      901 cggccttgct gctgccaaac cagtccaggc tgatactagt atcgcagaca ttcaaaaaag
      961 aggcgaactg gttgtcggtg tcaaacaaga cgttcccaat tttggttaca aagatcccaa
     1021 gaccggtact tattctggta tcgaaaccga cttggccaag atggtagctg atgaactcaa
     1081 ggtcaagatt cgctatgtgc cggttacagc acaaacccgc ggcccccttc tagacaatga
     1141 acaggtcgat atggatatcg cgacctttac catcacggac gaacgcaaaa aactctacaa
     1201 ctttaccagt ccctactaca cagacgcttc tggatttttg gtcaataaat ctgccaaaat
     1261 caaaaagatt gaggacctaa acggcaaaac catcggagtc gcccaaggtt ctatcaccca
     1321 acgcctaatt actgaactgg gtaaaaagaa aggtctgaag tttaaattcg tcgaacttgg
     1381 ttcctaccca gaattgatta cttcccttca cgctcatcgt atcgatgcct tttccgttga
     1441 ccgctctatt ctgtctggct acactagtaa acgcacagaa ctactagatg atagtttcaa
     1501 gccatctgac tacggtattg ttaccaagaa atcaaataca gagctcaacg actatcttga
     1561 taacttggtt actaaatgga gcaaggatgg tagtttgcag aaactttatg accgttacaa
     1621 gctcaaacca tctagccata ctgcagatta aggaggacac cccatgatag atttatcatc
     1681 ttggacagcc tattttcagg attttggaca atttttcaat ggtttcctct ttactcttgc
     1741 cctagcgatt ggatccttta tcctcgccat ggtcttagga atcttctttg gtgccatgtc
     1801 aaccagtaaa cgtccaattt tacgcatttt agctcacatc tttgtggaat tttaccaaaa
     1861 cactcccctc ttggtgcagt ttgttattgt tttttatggc ctacctctta tcagtgacca
     1921 tactatcatg attccaattt attggacagc tgttctctgc atgggactct atcacggcgc
     1981 ttatatcgct gaggttattc gttcagggat tcagtctatt cctagtggtc agatggaggc
     2041 cgccttgtcg caaggtttta cctatatcag tgccatgcgc ttgattatct tgcctcagac
     2101 cttccgcatc attctccctc cattgaccaa ccaaattgtt aacctcatca agaacacctc
     2161 tactgtagct atcatctctg gagtagactt gatgtttgtg actaagtctt ggtcggctct
     2221 caacggaaac tatatcccag cctttttagg tgctgctctt ctctactttg ctctatgctt
     2281 ccctgttgcc cagtttggtc gcaagatgga gcaagccaac aaaaaagcct attcacttta
     2341 ggaggttact atggaatcta ttttagaagt tttaacccca gataacctag tctttatctt
     2401 taaaggattt ggcttgaccc tctatatttc tctgattgcc atcatcctct ctactatcat
     2461 cggtacggtg ctagctgtca tgagaaatgg caaaaatcct gtcttacgca ttatttccag
     2521 tatttatatc gagtttgtgc gcaacgttcc caaccttctc tggattttta ctatcttttt
     2581 ggtgttcaaa atgaaatcca caccagcagg tattacagcc tttactctct ttacatcagc
     2641 agccttggct gagattattc gaggcggtct caataccgta gacaagggac agtacgaagc
     2701 aggaatgtca caaggcttca cctcagccca aatcctctac tacatcattc tcccacaagc
     2761 catccgcaaa atgctaccag ccatcatttc tcagtttgtt accgtgatta aggataccag
     2821 tctcctctac tctgttatcg ccctacaaga actctttgga gccagccaaa ttctcatggg
     2881 ccgttatttc gaaccagagc aggtcttcag tctttgcatc ctgattgccc tcatctactt
     2941 cagctttaac ctagcaattt ctagcctgtc tcatatgcta gccaaacgtt ggcaacaagc
     3001 tgcagaataa tactcttcga aaatctcttc aaaccacgtc agcttc
//
LOCUS       DAVBCG010000162         2960 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVBCG010000162 DAVBCG010000000
VERSION     DAVBCG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.162"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            111..740
                     /locus_tag="VQ495_001942"
     CDS             111..740
                     /locus_tag="VQ495_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2531857.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(863..2755)
                     /gene="pepO"
                     /locus_tag="VQ495_001943"
     CDS             complement(863..2755)
                     /gene="pepO"
                     /locus_tag="VQ495_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2531858.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT          789 a          693 c          593 g          885 t
ORIGIN      
        1 aagctagccg caggctgttc aaagcactgc tttgaggttg cagataaagt tgacgcggtt
       61 tgaagagatt ttcgaagagt attagtacat tctttgagat tggagttagt atgaaaatcc
      121 ataaaaccgt gaatcctgtt gcctatgaaa atacctatta tctagaaggc gaaaagcacc
      181 tcatcgtcgt cgatcctggt agtcattggg aagccattcg tcagacaatc gagaagatca
      241 acaaaccgat ctgtgctatt ctcttgaccc acgcccatta tgaccatatc atgagtctgg
      301 acttggttcg cgagacgttt ggcaatcctc ctgtctatat cgcagagagc gaagccagct
      361 ggctctacac tcctgtcgat aatctctccg gtctccctcg ccacgatgat atggcagatg
      421 tggtcacaaa acctgcagaa cacacctttg tctttcacga agaataccaa ctagaggaat
      481 ttcgttttaa ggttctaccg accccagggc actctatcgg tggtgtttcc ctagtctttc
      541 ctgatgctca cctagtcttg acgggagatg ctctattccg cgaaactatc ggacggaccg
      601 accttccgac tggtagcatg gagcaactcc ttcatagtat ccagacccaa ctcttcaccc
      661 taccaaacta cgatgtctat ccaggacatg gtccagctac tactatcgct cacgaaaagg
      721 ccttcaatcc ctttttctag caagatgatg acaatcgaaa tttaagtaaa ctatccagca
      781 aatctttcta ttacaaaagg catcctatca aggttttcac acgtgattgg atgccttttt
      841 tctgatgact agattttttg tattaccaaa taatcacgcg ctcctctggt gaacgccaca
      901 ttccgtctcc ttctttgaca tcataggttg taaagaaatc gtcgaagttt ggtacttgca
      961 cattgacacg gagtttggct ggtgcgtgca catcgacgct agccaaaagt ttcataaatt
     1021 ctggtcgacc tttcatgcgc cagatgcgac cgaagttgta gaagaactct tctgctgaga
     1081 agtctgcttc tctcttagct gcttcaagcg ctgctgcgat tcctcccaag tcagccacgt
     1141 tttctgatac agtcaattta ccgttaatgg ttgctccata agaatcctgt ccatcaaatt
     1201 ggtcaatgac tttttgtgtt ttctccttga aggcagcata gtcgctctct gtccaccaat
     1261 ccttgaggct accattttcg tcaaaggaag ccccgttagt atcaaaggcg tgggaaattt
     1321 catgggcaat cactgcccca ataccaccgt agttagcaga agatgactga tgcaagtcat
     1381 agaaaggcgc ctgtaaaatg gccgctggaa agacaatcag gttcttctga ggattgtagt
     1441 aggcattgac catatgagca ggcatgcccc attccttata gtctacaggc tggttccact
     1501 tactccaact gtgcttgatt tccacacgcg caaaggctag agcattctca aaaagactgg
     1561 cagtttcatt cactacctta tccttgtaac gtgcaggcaa ttcttctgga tagccaatat
     1621 aaggtttgat cacattgagc ttcacgatag cctgtttaca ggtttctgga gtgagccagt
     1681 cattcttaag cagacgctcc ttatagacat caatcatggt tgccactttt ttctccacat
     1741 ccgccttggc ttctggagag aacttctcac gggcgtacca aagccccagg gcttgcttga
     1801 aaggttcctg tgctagatga taagctgctt tgaccttatc ttttgcctct ggaactccag
     1861 aaagggcacg gctgtaggca ccagacaaaa cacggatatc ctctgttaaa tagctggttg
     1921 aaagattgac aacactcaaa atcaaggttg ctttaaggag agaccaggct tcctcactgt
     1981 aaaattgctc tgctgcttgc cagaaacgtt cctcgtctac aataaccttg tctggtaatt
     2041 gcccaataac tgctttgaag aagtcatcca aaggtagggc aggcgcgaat ttcttgaaat
     2101 cttcgtaaga atatggatga tagagtttag catattctga actttcttca ttagagagca
     2161 ccactgccgc aactcggcgg tccaattcaa gtcttttttc tagcaagtct tcaatttctt
     2221 catcagagaa atcataagcc ttgaggagat ttgcgctgct ttctttccaa agagtcaaga
     2281 gctcttcgcg ctgaggatgt tcttctgcat agtaggtcgt atctggcaag attgtgcttg
     2341 gagcgctagc ccatagaaca ttgattctag catccataaa gtctggcgat acaccaaaag
     2401 gaaggaagtt tggttttcct gcaagctcaa actctgctag tttagctgta aaatccgcaa
     2461 aagtttccaa ttcttggaat tctttaagga gtggtaagac aggtgtgata ccgtcagctt
     2521 ctctcttgtc aaaatcacga actaggcggt ggtatttgac aaagttttcc aagatagcat
     2581 cctcaggcac ttcttcacct gctaaccact tgtctgttgt cgccagcatc aggtcttcaa
     2641 tttcctggtc taaatcaaca aaacctcctg tttgagactt atctgctggg atttcagctg
     2701 tctgttgcca ttctccattg atagcatcat aaaaatcatc ttgataacgt gtcatcttgt
     2761 tctcgctttc atttgtattt gcatttatct taacaaaaat cactagggaa tgcaattaat
     2821 aaccatttaa ctattatttt ttagctatta taaaaaacct ttccatgata aaacgcataa
     2881 ttccaagttt tttacacttg atactatgcg ttttataatt tagaaattta tcctgaaagt
     2941 tatctttaga atctattttt
//
LOCUS       DAVBCG010000163         2891 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVBCG010000163 DAVBCG010000000
VERSION     DAVBCG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.163"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(91..1140)
                     /locus_tag="VQ495_001944"
     CDS             complement(91..1140)
                     /locus_tag="VQ495_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2531859.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(1137..1868)
                     /locus_tag="VQ495_001945"
     CDS             complement(1137..1868)
                     /locus_tag="VQ495_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2531860.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            1936..2346
                     /locus_tag="VQ495_001946"
     CDS             1936..2346
                     /locus_tag="VQ495_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2531861.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            2356..2643
                     /locus_tag="VQ495_001947"
     CDS             2356..2643
                     /locus_tag="VQ495_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531862.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT          967 a          649 c          549 g          726 t
ORIGIN      
        1 tctctacgga ccaaccatta tcctgtagta acaaaaaaga agctgagaca gactgaactc
       61 aacttctttt ttagtgtcgt atcagcaatg ttagtcctgc atctgacgtt ttacctgata
      121 aggcaaatac aagactagta aaaccaaacc agctcctagt aaggctagaa gggccagttt
      181 ttcttggaag gtaatcaacc caacaactaa ttccacaagt aaaacaaaac tggtcaatcc
      241 tcgaactacc tcctgtaagc ctttttcctt ttgccccttg tccagcggaa agagttgggt
      301 caaatactgg tagtcaaagg catgatagag ggccagcaac tggaagagca agaggtagtt
      361 aaagagaact accactgctg tcgcaatcca agcttgctcg ataaaaacct gcgccagtac
      421 ggaaagtaat agaagacgaa gactgagagc aaagaggtcg ccatttcgca gataagaacg
      481 cagatagaga ttttgccaaa tcttcccagg caccttctga acagccttta agataaagtc
      541 cagataggca cgacgcttaa cgctgtttga aattcccttg acctgcgtaa agagggcaaa
      601 gaaacgaagc aagacttgct tacgcttgct ttcttgagaa ataacatagt cccagtccag
      661 tccagtttca gtaaaaaatt tgctggcctt ttgacgaaag tggaaatatt ttcctacccc
      721 caataaaagc acatatagca gaaaaactgg caagccataa cccattgcta aaaataaagg
      781 cgcaaataac agcaagaaaa gggtctgtac aaagagccaa aagactaggg aaatgccagt
      841 ttgacgcttg agatggagct tgattgcctc ttctccaact aagagaaaga gcttgtctgg
      901 agcctccata taggtggcaa ttcctcccca aagtaagagt aaaacagacg taattcctac
      961 aaacaaaagg ataggccaat gattttcagg aaaatgttgt aagagttgac tgtactggta
     1021 ggctagaaag cccaacagga caagcaggaa caagacaaag tggtcattga gcacatagcg
     1081 cagataacaa agacactcct tacgaaaggc ctgctttctc tttaaaaaca agtctttcat
     1141 agatcctcct ctttggtcag agccaagtaa atatcattca aactagcctc aggcatatca
     1201 aaggcttcac gtagttgcag gagattgcct ttggcacgca cctctccctt gtgaagaatg
     1261 acaaaggcat cacacatctt ttccgccgaa tccagcacgt gggtactcat gagaatagac
     1321 ttgccctttt gcttctccac ttccaaaagc tgaatcaaat cagaaatagc cagcggatca
     1381 agaccaagga aaggctcatc cacgatgaaa agacttggat ccaccacaaa agcacagata
     1441 atcatgacct tctgcttcat cccttttgaa aaatgaacag ggaaccagtc taatttctgt
     1501 tccaaacgga acatttttaa caagggctct actcgttcga aagccacttt ttgctcaata
     1561 ccgtaagcca tagcaaccgt ttcgatatgc tctctgaggg tcaattcctc atacagacta
     1621 ggcgtctcag gaatgtagcc aatctgcttg cggtagctag tcgcatctcc ttgcagagtc
     1681 aggccattga tattgatgga gccactataa ggtgccaaca gaccgataat ctcattgatc
     1741 gtcgttgatt tcccagcacc attgagacca atcaaaccga ccaactgccc actttcaaca
     1801 gtaaaggaca catctttcaa aacaggaaca tgaacatagc cacctgtcag gtttttaatt
     1861 tctaacatat tttctccaaa tctggtataa tgtagctata ttatatcaaa attcagtaca
     1921 gtagaggtag attttatgtc agattgcatt ttttgtaaaa tcatcgcagg ggaaattcct
     1981 gcttcaaaag tatatgaaga tgagcaggtc cttgcctttc ttgatatctc tcaagtaaca
     2041 ctaggacaca ccttggtcgt gccaaaagaa cactatcgca atcttttgga gatgaatgct
     2101 acgagcgcca gccaactctt tgcccaagta ccaaaagtag ctcaaaaagt catgaaagtc
     2161 actaaggctg ctggtatgaa tatcatttcc aactgtgaag aagtcgctgg tcaaacagtt
     2221 tttcatactc acgttcacct tgtgcctcgc tacagtgctg acgatgacct caagattgat
     2281 tttatcgccc acgaaccaga ctttgacaaa cttgctcaag tcgctgaaac catcaaaaac
     2341 gcttaaggag ttgctatgaa attatccaac ctactgctat ttgcaggagc tgcagccgga
     2401 agttatctgg ttacaaaaaa tcgccaaacc atcacagatg aagtcttgaa taccactgac
     2461 cgcgttcaag ctatcaaaga cgatgtggat attatccaaa atagcctgca aatcatcgac
     2521 cagcaaaaag aacttatcaa ggaataccaa gaagacttga cttacaagtt taaggtcttg
     2581 gaaaaggata tccagactag actagctgtg ataaaagaaa tgcagggaac tgaagataag
     2641 taaaaagagc cccccggctc ttttttaaaa cgtaaacaag ttatagtgaa ttgaatttag
     2701 aatagcacat catagtttct aaagcattgt tagagattac tttaaaatcc tcttatcaat
     2761 ttgttcatat tctatttcaa tctactatat atatcccatc aactatgcat cataatttag
     2821 gtaactcata ctcaataaaa atcaaaaagc aaactagaaa gctaggcaca gactgctcaa
     2881 aacaccgttt t
//
LOCUS       DAVBCG010000164         2860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVBCG010000164 DAVBCG010000000
VERSION     DAVBCG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.164"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(4..945)
                     /locus_tag="VQ495_001948"
     CDS             complement(4..945)
                     /locus_tag="VQ495_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2531863.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(923..2686)
                     /gene="aspS"
                     /locus_tag="VQ495_001949"
     CDS             complement(923..2686)
                     /gene="aspS"
                     /locus_tag="VQ495_001949"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2531864.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGTVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKEAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVFFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETVHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          829 a          678 c          569 g          784 t
ORIGIN      
        1 tcactaatta tctatctcaa caaatcttcc tagaatatga acattttccg agacagagac
       61 aaaggagctt ggatccactt gtgtcataat ctgtttaaat tcattaaact ctgcacgtgt
      121 aatgacagtg attaaaactg cctttctctc gtgattatag gttccttctg catcgtggat
      181 catggttgct ccgcggtgca attttttatg gattttttca attaccttct ctggatgatt
      241 tgtcacaatc atggcctgca tacgcttttg cttagtaaag actgcgtctg tcacacggct
      301 agagacaaag atggtaatca tagaataaag agcgtatttc caaccaaagg tcaaacctgc
      361 tatcagcatg atagttccat ttaccaagaa agaaatacta ccgacattct tacccgtttt
      421 cttacgaata gtcaggctga cgatatccgt cccaccactg gagatattgt ttcgaagagc
      481 aaaaccaatc cccaaaccca taacaacacc cccaaaaagg gaattgataa tgggatcctc
      541 cgtcaaggtt gccacaggga caaactggat aaagaaggaa ctcatagata ccgtgataaa
      601 ggtaaagacg gtgaacttat ggccaatctg ataccaagct aagaccatca aagggaagtt
      661 aatggcgtag aagcttagcg aaatcggaat atgaaaacca aaccagtgat tactcaaggc
      721 agagataatc tgtgccagac ctgttgcacc actcgaatac acatgccctg gttggaaaaa
      781 gaaattaact gctactgctg ataaaaaacc atagaccaga gaggccgaaa tcttctcatc
      841 atacttttct cgagagatac tttgtaagac acgtaaaatt tttatctgat aagcaaagcg
      901 gcgcagataa tagcgccacc gcttaattcg ttttgtttgt ttcatcttct tctacttgta
      961 agctgagttc ctctagttgt ttgagagcga ctgttgatgg agcttgtgtc attgggtcag
     1021 ttgccttgtt gttcttagga aaggcaatga cttcacggat attttcttct ccagcaagca
     1081 acatgacaaa acggtcaagc ccgatagcca aaccaccgtg tggtgggaaa ccatagtcca
     1141 tggcttcaag aaggaaacca aactggtcat tggcttcttc agttgagaaa ccaagagcct
     1201 tgaacatgcg ttcttgaagg tctttttggt tgatacgaag gctaccacca ccaagctcat
     1261 aaccgttcaa gacgatatcg taagcaatgg cacgaacctt agccaaatca ccttctaatt
     1321 cgtgaacagt ctcttcctgt ggaagggtga aaggatggtg ggcgctcatg tagcggcctt
     1381 cttcttcaga ccattcaaac atcggccagt caaccaccca aaggaagttg aacttatcat
     1441 tatcaatcaa gccaagctct ttagcaatac gtccacgaag ggcacccagt gttgcattag
     1501 ccacttcaag cgtatccgcc acaaagaaaa ccaagtcctt atcttcaaga gcaagcgctg
     1561 ttgtcaattc ttcttggata ccagtcaaga acttggcaac tggtccgttt aattctccat
     1621 caaccacctt gacccaagca agacctttgg caccatactg tttggctact tccgtcatct
     1681 tgtcgatgtc tttacgtgaa tagttgtccg cagcttcttt gaccacaatc gcttttacag
     1741 caggtgcttc tgaaaagact ttaaagtcta cacctttgac cacttctgtc aagtcctgaa
     1801 gcaacatgtc aaaacgagta tctggcttgt cagaaccgta aagagccata gcatcatcgt
     1861 atttcatacg agggaatggt agcgttactt cgatgccttt tgtttccttc atcacgcgcg
     1921 cgatcaagcc ttctgtaata tcttggattt cttgctcagt aaggaaggac gtttccaagt
     1981 cgacctgagt aaattcaggc tggcggtctc cacgcaagtc ctcgtcacgg aaacatttaa
     2041 cgatttggta gtaacggtca aaaccagcat tcatcaagag ctgtttcgtg atttgtggac
     2101 tttgaggaag agcgtaaaaa tgccccttat taacacgaga cggcactaaa taatcacgcg
     2161 ccccttcagg cgttgactta gaaaggaatg gtgtctccac gtcgataaac tccaactcat
     2221 ccaagtagtt gcggatagag tgggtcacct tggcacgaag tttaagattt tccaacattt
     2281 ctggacgacg aaggtcaagg taacggtaac gcaaacgtgt atcgtcattt gcctcaatgc
     2341 catccttaat ctcaaatggt gttgtcttag ctgtgttaag cacaataaga gctgtcacgt
     2401 ttaactcaac cgtaccagtt ggcaacttat cattggcttg ctcacgcgca gcgacctgac
     2461 cagtcacctc aataacaaat tcgctacgaa ggctttcagc tgttgccata acctctgcag
     2521 atactttttc agggttgata accaactgca tgattccttc acggtcacga agatcgataa
     2581 agatcaaacc accaaggtca cgacgacggc caacccatcc tttcaaggtt atttcttgtc
     2641 cgatgtgttc ctcacgaaca cgaccagcat acatactacg tttcattatt tctctcctct
     2701 tttattctgt tactatttta ccataaaagc gcaggctctt catgaaaatc atcagaaaag
     2761 tttgccagtc tttaaaagtc aggtgaaagc cctaaaaatt agcgctaata ctcttcgaaa
     2821 atctcttcaa accacgtcag cgtcgccttg ccgtaggtat
//
LOCUS       DAVBCG010000165         2848 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVBCG010000165 DAVBCG010000000
VERSION     DAVBCG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.165"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            29..721
                     /locus_tag="VQ495_001950"
     CDS             29..721
                     /locus_tag="VQ495_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2531865.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            764..1990
                     /locus_tag="VQ495_001951"
     CDS             764..1990
                     /locus_tag="VQ495_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2531866.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
BASE COUNT          843 a          565 c          593 g          847 t
ORIGIN      
        1 tagtataata aaaacaaagg agaacagcat gctgaaatgg gaagacttgc ctgtggaaat
       61 gaaatcaagc gaggttgagt cttactacca gcttgtctct aaaaggaagg gttcgctgat
      121 tttcaagcgt tgcttggact gggttttggc cttggtctta ctggttctga cctctcccat
      181 ctttctcatc ttgagcattt ggatcaagtt ggatagcaag gggccagtga tttacaagca
      241 agagcgtgtg acccagtaca accgtcggtt caagatttgg aagttccgta ccatggtgac
      301 ggatgcggat aaaaaaggaa atctggtgac ttctgctaac gatagccgta ttaccaaggt
      361 tggaaatttc atccgacgtg tccgtttgga cgaactgcct cagttggtca atgtccttaa
      421 aggtgagatg tcctttgtcg gtacacgacc tgaagtgcca cgttatacag agcagtatag
      481 cactgaaatg atggcaacct tgctcttgca agcaggaatt acctctccag ccagcatcaa
      541 ctacaaggat gaggacacca tcatcagtca aatgacagag aaaggtctgt cagttgatca
      601 ggcctatgtg gagcatgttc ttcctgaaaa gatgcgctat aacctcgcct atctccgaga
      661 gtttagtttc tttggggaca tcaaaatcat gtttcaaacc gtgtttaagg tactaaaata
      721 aagtagtcat aagagaatga gtacagataa aaggagcaaa tcaatgccaa attacaatat
      781 tccattttca ccgcctgata tcacagaagc agaaattgct gaagtagcgg ataccctgcg
      841 ttctggttgg atcacaacag gtcctaaaac aaaagaactg gagcgccgct tgtctcttta
      901 cacacagaca cctaagactg tttgtctcaa ctctgcgaca gccgctctgg agttgatttt
      961 acgcgttttt gaagtgggac ctggtgatga agtcatcgtt ccagccataa cctatacggc
     1021 ttcatgtagt gtcattacgc acgtgggagc aacccctgtc atggtggata tccaagcaga
     1081 tacgtttgag atggactatg acctgcttga gcaagctatc actgagaaaa ctaaggtgat
     1141 tatcccagta gagctcgcag ggattgtttg cgattatgac cgtttgttcc aagtcgtgga
     1201 gaaaaaacgt gacttcttta ccgcttcaag caagtggcaa aaggccttta accgtattgt
     1261 cattgtctct gatagtgccc acgctttggg atctacttat aaaggacaac cttctggttc
     1321 tatcgctgac tttacttcct tctcattcca tgccgttaag aactttacaa cggcagaagg
     1381 tggaagtgcg acttggaaag ccaatccagt gattgatgac gaagagatgt acaaggaatt
     1441 ccaaatcctt tcccttcacg ggcaaactaa ggatgctctt gccaagatgc aactggggtc
     1501 atgggaatac gatatcgtta caccagccta taagtgcaac atgaccgata tcatggcttc
     1561 acttggtttg gtacaattgg accgctatcc aagtttgttg caacgccgta aggacattgt
     1621 ggaccgctat gatagtggtt ttgcaggttc tcgcatccat cctttggcac acaagactga
     1681 aactgtcgaa tcttcacgcc acctctacat cacccgtgta gaaggagcaa gcctagaaga
     1741 acgcaacctc atcatccaag aattggctaa agcaggaatt gcaagtaatg ttcactacaa
     1801 accgcttcct ctcttgacag cctataagaa tcttggattt gatatgacga actatcctaa
     1861 ggcctatgcc ttctttgaga atgaaattac cctccctctt catactaaat taagcgatga
     1921 agaagtagac tatatcattg agactttcaa aacagtttct gaaaaagtgc taactttatc
     1981 aaaaaaatga caaactacag tcaagcgaaa gtgatcctgt ccctaaaaag tctaattgag
     2041 tgtaaaaact gttgttttca attgataata gtttacacct gtagttgagg cctctctctc
     2101 ctcagagaga gaatttttat aggattttcc tttcttgtgg gagtcccgtg gtttgaaata
     2161 agatgtgagc aatttggtgt agcatttaga atccttacta gacatcattt agaaaatcta
     2221 gtgtcttgtt ctagttttta attcacccta ttttttgaaa gacgtgagtt tccatgagtg
     2281 agattgtgga aactcgcgtc tttttttgtt ttcagaatat tgttcaaagt tttgtgcctg
     2341 tctttcatgt tctagtcatt cttttgcatg atagaattta tagcatattg acattataat
     2401 aacacaaata ttctatatgt ttagtgatgc ttgctataca tcattagatc tcccgcgaga
     2461 caatctataa aacacttgtc tacgattacc tatatgccct attccagtat tttagaagca
     2521 ctgcatctat ttttatcgag gttaaatcta gcttttatag aaggtctatt taagaaatat
     2581 attgtagtgt tttagtttca atccgccata tgagcgatat tcatgtaaat atccctggcg
     2641 aatgcttgta tgacaagata tttgttcttt catttataat ttacaacata tcaacaaatt
     2701 taaatatagt aaatgggata ttttatattc aagctaagaa agatagcatc acttttgaat
     2761 ggaaggctaa agagcaaact aggaagttgg ccatagatag ctcaaaaccc tgctttgagg
     2821 ttgtagatat aataaaatga aataagaa
//
LOCUS       DAVBCG010000166         2822 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVBCG010000166 DAVBCG010000000
VERSION     DAVBCG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.166"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..916)
                     /gene="cysK"
                     /locus_tag="VQ495_001952"
     CDS             complement(<1..916)
                     /gene="cysK"
                     /locus_tag="VQ495_001952"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2531867.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYE"
     gene            complement(1201..1836)
                     /locus_tag="VQ495_001953"
     CDS             complement(1201..1836)
                     /locus_tag="VQ495_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2531868.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITTIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            1892..>2822
                     /locus_tag="VQ495_001954"
     CDS             1892..>2822
                     /locus_tag="VQ495_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2531869.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASE"
BASE COUNT          835 a          541 c          559 g          887 t
ORIGIN      
        1 attcataaag tgctgtagag agataacgtt caccgttatc tggtgctagg gcaaggactt
       61 ttttacctgt acctaatttt ttggcaacct cgatggctcc gtagatagct gcagctgagg
      121 aaatccctac aaggaagcct tcttttccac caatttcacg tccgagtgca agagcgtcat
      181 ctgatgttac acgaacgata ccatcatagg ctttagtatc aagtgtatca ggaataaatc
      241 cagctgagat accttgaatt ttgtgaggac caggtttttc accagataga atagcagatt
      301 catctgcttc tactgcaaaa acttgaatgt tagaattttc tgatttgagt gcatgagaaa
      361 caccagaaat cgttccacca gtacctactc cagcaacaaa ggcatctaat ccatctttac
      421 cgaaagcagc tagtatctca gctcctgttg ttctttcgtg tacttctgga ttagctggat
      481 tgtcaaattg aagaggaagg aaaccatcac gttcagcagc gatttcttga gccttagcaa
      541 tagcaccttt cattccctcg ctaccaggag ttaggacgag ttcagcacca taagcttgga
      601 taattttacg tcgttctaca ctcatagttt caggcataac gatgacgact ttataccctt
      661 tagcagcacc tacccatgaa agtccaatac cggtgtttcc acttgttgct tcaacaatag
      721 tagaaccaag tttcagaata ccatcttgtt cagctttttc aatcatgcta agggcaatac
      781 ggtcttttac agatgaacca ggattaaatg cttcaagctt tatatagacg tctgcagcac
      841 cttctggcac gatgttgtta agtttaacaa tcggtgtttg accgattaat tcagtaatgt
      901 tgttataaat agacatatga atacctcttt gtataagata tctttagtat aacgctatcc
      961 ataccatttg taaaatatag aaatcctatc gaagcgatag gatttctata tatcatagat
     1021 caaagtcatt aattttcaag tgattgcgtt cagttaattc tagtaaatag gtgtgaagga
     1081 aaacgatatc aggtttcgtt tgaattgctt cattttctag tatattttgg tacgcatctt
     1141 tttattttgc tcgctaaagt agttgtgaga atgtcaacga aacgctgtat tattcattgt
     1201 ttacactaag tttacaggaa cttcaacctc tcgtaaacct tggtcagtta aagtgacttt
     1261 tccattaaaa aactccacaa gtgcagcttt aatagtttct ttttcttctt tatcaacata
     1321 aatcatcgta tcaacttgat ctgtaaagtt tgtatccagc tccatgagat catgttcttt
     1381 aaggaagtta ctgtactctt ggtactgagc ataagacatt tgaatagcaa tgccagcctg
     1441 ttcttttatt tcaataatac caatttcttt gacagctaag gcgacactgc cggcgtaagc
     1501 acgaattagt cctccagcgc ctagtttaat accaccaaag tagcgtgtca cgaccacaca
     1561 gacattggtg agattgtgat tttctagtac cccaagcatg ggaacaccag cagtaccact
     1621 aggctcacca tcatcacttg tacgtttaat ttcactacgt tctccaataa taaaggcaga
     1681 gcagttatgt gtcgctttgt agtgttcttt tttgatggta gtaatgaagt cacgagcctc
     1741 ttcttcgcta taaacacgct tggcatggca gataaagcga gattttttga tttcttcttg
     1801 gacttggccg tcctctttaa ttgtcctaaa ttccatgatt taattgtact aaaaaatcat
     1861 ctaaagtgct agtttttacg aataaagaag tatgaaagta aatttagatt atctcggtcg
     1921 tttatttact gagaatgaat taacagaaga agaacgtcag ttggcggaga aacttccagc
     1981 aatgagaaag gagaagggga aacttttctg tcaacgttgt aatagtacta ttctagaaga
     2041 atggtatttg cccatcggtg cttactattg tcgagagtgc ttgctgatga agcgagtcag
     2101 aagtgatcaa actttatact attttccgca ggaggatttt ccgaagcaag atgttctcaa
     2161 atggcgcggc caattaactc cttttcaaga gaaggtgtca gagggactgc ttcaagcagt
     2221 agacaagcaa aagccaacct tagttcatgc ggtaacagga gctggaaaga cagaaatgat
     2281 ttatcaagta gtggctaaag tgatcaatgc gggtggtgca gtgtgtttgg ctagtcctcg
     2341 catagatgtt tgtttggagc tgtacaagcg cctgcaacag gatttttctt gcgggatagc
     2401 tttgctacat ggagaatcgg aaccttattt tcgaacacca ctagttgttg caacaaccca
     2461 tcagttattg aagttttatc aagcttttga tttgctgata gtggatgaag tagatgcttt
     2521 tccttatgtt gataatccaa tgctttacca cgctgtcaag aatagtgtaa aggagaatgg
     2581 attgagaatc tttttaacag cgacttcgac caatgagtta gataaaaagg tccgtttagg
     2641 agaactaaaa agactgaatt taccgagacg gtttcatgga aatccgttga ttattccaaa
     2701 accaatttgg ttatcggatt ttaatcgcta cttagacaag aatcgtttgt caccaaagtt
     2761 aaagtcctat attgagaagc agagaaagac agcttatccg ttactcattt ttgcttcaga
     2821 aa
//
LOCUS       DAVBCG010000167         2781 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVBCG010000167 DAVBCG010000000
VERSION     DAVBCG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.167"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            87..>2781
                     /locus_tag="VQ495_001955"
     CDS             87..>2781
                     /locus_tag="VQ495_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET2531870.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSDHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEI"
BASE COUNT          856 a          451 c          609 g          865 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctgatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcat
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcga atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caaggattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tgtttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgacca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt a
//
LOCUS       DAVBCG010000168         2762 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVBCG010000168 DAVBCG010000000
VERSION     DAVBCG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.168"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(115..1356)
                     /locus_tag="VQ495_001956"
     CDS             complement(115..1356)
                     /locus_tag="VQ495_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2531871.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            1459..2568
                     /locus_tag="VQ495_001957"
     CDS             1459..2568
                     /locus_tag="VQ495_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2531872.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          807 a          467 c          529 g          959 t
ORIGIN      
        1 acctgcggct agcttcctag tttattcttt gattttgagt attagattta ctcaaaatca
       61 aggattttga agatgaactt gtatcaaaaa atcgcggttt aaaatcgcga ttttttataa
      121 tttctcgtta acaaagcgga caaactgatt ccaccaaact tttaagaaga aggctttttc
      181 aattttcttg tctgctacca tttcgaaact agggcgctct gtggtgatgt aaccttgacc
      241 aatcaagtcc ttgtcttcat aagtcaaatg gccaaccact gttccagctt caagtggtgc
      301 tgggattgct ttggaatcag gtgtgaattg aacagattgg gaagattgat tcccaacacg
      361 ttcgattaga tagatatcct ctggagccac tgcagttact gtatcttctt ttccatcttg
      421 tacaggggct ttgctatctt gataggcatc gccttgttga acgattttgc gaagtgtaaa
      481 tgtagaagaa atataatcca ttagggaaga tgtagctgta aatcgagcgt aaggattatt
      541 gtcttgatga tctgcattta aaacaactgt gatgactctc atgccttttt cgacagtagt
      601 accaacaaaa gactctccag ccttatctgt tgttcctgtt tttagcccat caaaaccacc
      661 acggtaagca ggcatacctt ctaacatgta gttggttgaa gtgattgtca tcccagcaaa
      721 agtagaagaa ggttttttgg tgatttctaa gacttgtggg tattttttga tgaggttgcg
      781 agcaacgata gcgacatcat aagcactaag cttattttcc tcatcttttt tagaacctgg
      841 gtaaatgtta tcccctagag tttcattgtt aagacctgtc gtattgacaa cagtggcatc
      901 ctgaattccc cattccaaga gttttgcccg catcatatcg atgaaatctt tttctgagcc
      961 agcaattttc tcagctaggg caatagcggc gctgttggca ctagatacca gagttgcttc
     1021 aagcaactct tcgacagtat aattacgggc ctccatagga atattactgg cttcagaatt
     1081 tgtcgtcaat tgataaggat aatcagaaat atctacagga gtggagaggg taatacttcc
     1141 gttttccaaa gcttcataga ccagataagc agtaatcaat tttgttatgg aagcaatttc
     1201 gacaggttgc gttgcatcct tctcatagag aattttacca gtatttgcct caacagcaat
     1261 cgcatgttta gcggcaatgg taaaatcttg agcaacagca gtagaagcac cccctaaaag
     1321 agagacagtt aacaaagtta aaaatatttt tttcatagta gtcttattct atcataaaga
     1381 aaaaaaatat tcttgcttta ataattcatc tgttaagctt tttgaaaata tggtaaaata
     1441 aagtgaggga ggtaactcat gtttcgtaga aataaattat ttttttggac cacagaaatt
     1501 ttactcttaa ccatcatctt ttacctatgg agacagatgg ggtctttgat taaccctttt
     1561 gttagcgtgc ttaatacaat tatgattcca tttttattag ggggctttct ttattatttg
     1621 acaaacccta ttgttacttt cttaaataaa gtctgtaaac tcaatcgttt gcttggtatt
     1681 ttaattacct tgtgtacttt ggtctgggga atggtcatag gtgttgtcta tctcttacct
     1741 attttgatta atcagttatc tagtttgatt atatctagtc aaactattta tagtcgagta
     1801 caagacttaa tcatagactt atctaattat cctgcgctcc agaatttgga tgtagaagct
     1861 acaattcagc agttaaactt atcctatgtt gatattcttc aaaatatcct aaatagcgta
     1921 tcaaatagtg tggggagcgt cttgtcagct cttatcagta ctgttttgat tttgattatg
     1981 actccagttt ttttggttta tttcttatta gatggacata aattcttgcc catgcttgaa
     2041 agaacgattc taaagaggga tcgcttgcat attgcaggct tattaaagaa tttaaatgcg
     2101 acgattgctc gctatattag tggagtttcg attgacgcaa tcattatagg ttgtttggct
     2161 tatattggct atagtattat tggtttaaaa tatgctttag tttttgccat tttttctggt
     2221 gtagccaatt taattcctta tgtggggcca agtattggtt tgattcctat gatcatcgca
     2281 aatatattca ctgatcccca tagactgctg attgcagtga tttatatgct tgttgttcag
     2341 caggtagatg gcaatatctt atatcctcga atcgtaggaa gtgttatgaa ggttcatcca
     2401 atcacgattt tagttttact tttgttgtca agcaatatct atggtgtagt tggaatgatt
     2461 gtcgcagtgc caacctattc tatcttgaaa gaaatttcta agttcttatc ccgtttgtat
     2521 gaaaatcata aaataatgaa agaacgagaa agagaattag ctaagtaaaa gtcaggagaa
     2581 ccctgatttt tctttactgg aagtggcctt tagattagaa gactgaaaat aagttaaagt
     2641 cttaaactaa ttttcacagc taagaatagt agaagttaat ctgataaaaa tcgaaaaaac
     2701 cagtggaatt ctgtgtcagg gtaagttcca ctggttttca tagtctatta aagttcgaat
     2761 ga
//
LOCUS       DAVBCG010000169         2761 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVBCG010000169 DAVBCG010000000
VERSION     DAVBCG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.169"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(264..1574)
                     /locus_tag="VQ495_001958"
     CDS             complement(264..1574)
                     /locus_tag="VQ495_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2531873.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1811..2683
                     /locus_tag="VQ495_001959"
     CDS             1811..2683
                     /locus_tag="VQ495_001959"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2531874.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          834 a          478 c          649 g          800 t
ORIGIN      
        1 tctcaaattg agtatgacga agtgcgctcc catgtcctgg gaacgcactt tcttcatatt
       61 tttcatattc ttgaatccat cgataaagac tattgggatg aatttttaaa gttgaactaa
      121 tcatttttac aggatgaggt ttacagcaga gagtttgaag gctttatcaa aggtttttct
      181 tggcataatg acttttcctc gtttccactt aattttgtgt ctactttatt ataccaagtc
      241 cacccttaag ttagataata aatctaactt aaggaagcta gaaggatgag aatccaggtg
      301 gtcaagagtc ccaaacttaa gctgattggg acacccagaa taatttgctt tttgaaggca
      361 aggccacgtt cctctatatt gggaagtgag agttgaatga gagaaccagc tgatgaaaag
      421 ggtgagatat tagtagatag agcgccaata acggtggctg ttgtgagtaa gtgaatatca
      481 atctggggat tttgagcact gatgatagca atgatgggaa agagggctgg agctacaacg
      541 gatagggtgg aactaaagag tgacatcacc ccggctatca cacaaaagaa cagaggtaac
      601 cagaaatgag gaatggatgt tgtcatgagg tgccctatca gtgtgactaa acctgacttg
      661 accgctagag acattagtaa gctcatgccg cagagcatga taattgtagc ccagggaact
      721 ttagccaaaa tggcttcttg cttccctaat ttgagcctta aggcgaggca gaccatgagt
      781 attgagacaa agccaatatc aaatgttttt cgataagtag ctatccaggc gatgtttggg
      841 aaaatgagat gcaacaaggg aaaaagccaa accaaaacca tgctgctgat catgagcaag
      901 gtggtttgtc tttgaacctt gctgaggagt ggtggttggt caatagtcaa ggatgagttt
      961 gttcttccct tactatagtg actgtaacag gataataaaa gcaagacaat gagtgggtag
     1021 ataatgctga cgataaagat atgattgcca agtgaaaaag cttgctcttc ccatcccatt
     1081 tgcttaaaca ggccttgaaa gacaatgcct gagctactgg ttatcaaatt agcccctcct
     1141 gaagctcccc aattgacggc ttgagctcca atcaaagggt gtttgtccgc tttttgacag
     1201 agagtaatcg ctagaggaca gcaaacggcc atagtagtga aaaatccagc acctaaagca
     1261 gacaaaaggg ttgccatcag gtataaaatc atgtagaggg cgttagggta ggtgcgtgtg
     1321 cggtagagaa tgtgttgagc caaaacatca agagtaccgt tagttattgc aaagttataa
     1381 aagagagaga cgctaaaaat ggtaaaaaag agtgaggttg gccaaaaatg aagaagttct
     1441 ttggggctta atcccatgag agtggttgcg atgaggtaag aaaaagcaat agccagcagg
     1501 ccaatattga ttttggtgcg gtaaccaatt ccaatggcta gagcaatggc gctaatcatt
     1561 attaaatgaa tcattgaatt gtcctttcag ttagaatatg gaaataggtt ggcaattttg
     1621 gcgctcaaaa gaccacctgt ttcctattgg tacttgacta ttatacacaa aaaaaataca
     1681 attgtcttga caattgtatt gacacttgtt agaattaaaa atgaggtatt caggcttgtg
     1741 atttcctatg gaagttaata gtgattgcct ctaatgctta caagtgatat taaaaataga
     1801 ggacctagtg atgtcaatca tttcaactga tttaacccct tttcaaatag atgatacatt
     1861 gaaagcagcc ttgcgagaag atgttcattc cgaagattac agtaccaatg ccatttttga
     1921 tcatcatggc caagccaagg tgtcgctttt tgccaaggaa gctggtgttt tagcggggct
     1981 aaccgttttt caaagggttt ttaccctatt tgatgccgag gtgaccttcc agaatcctca
     2041 tcaatttaag gatggggatc gtttgactag tggcgatttg gttttagaaa tcataggctc
     2101 ggtgagaagt ctcttaacat gtgaacgcgt tgccttgaat tttttacaac atttatcagg
     2161 gattgcttcg atgacagctg cttatgtaga agccttaggc gatgattgca ttaaggtatt
     2221 tgatactcga aaaactactc ctaatttacg tctttttgag aaatatgcag tgagagttgg
     2281 cggtggctat aatcatcgct ttaatttatc agatgctatc ctgctaaaag acaatcacat
     2341 tgcggcagta ggtagtgttc aaaaggcaat tgctcaagcg cgtgcctatg ccccttttgt
     2401 gaaaatggtc gaggtggaag tggaaagcct tgctgctgcc gaagaagctg cggcggcggg
     2461 tgctgatatt atcatgttgg ataatatgtc attggaacag attgaacagg ccattaccct
     2521 aattgcagga cgttctcgga ttgaatgttc tggaaatatt gatatgacca ctattagccg
     2581 ttttcgtggt ttagcgattg attacgtctc cagtggtagt ttaacccata gtgctaagag
     2641 tcttgatttt tccatgaagg ggttaaccta ccttgatgtc taagttgtaa aataaactaa
     2701 ctttttaaag gatgtctttc ctctagaacg agttttatgt cagatagttt aaacgcctct
     2761 t
//
LOCUS       DAVBCG010000170         2665 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVBCG010000170 DAVBCG010000000
VERSION     DAVBCG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.170"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            239..2563
                     /gene="pflB"
                     /locus_tag="VQ495_001960"
     CDS             239..2563
                     /gene="pflB"
                     /locus_tag="VQ495_001960"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2531875.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKSNFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT          844 a          554 c          522 g          745 t
ORIGIN      
        1 ttcgatttcc attgagtgtt actgcttatt ttcttttatt ataccctttt ttctgaaaaa
       61 aagaaaaaag gactttattt tttcaaaaat ataatacagt ttgaaataaa atatagactg
      121 ttttagaaaa gaaagtgtaa aaatagggaa ttttcacttg ttgaaatcgg ttactatatg
      181 gtatacttgt cttatgaatg taacagatga ctgttactag aaaaaagagg acattaatat
      241 ggttgttaag acagttgttg aagcacaaga tatttttgac aaagcttggg aaggcttcaa
      301 aggcgtagat tggaaagaaa aagcaagtgt atcacgattt gtacaagcta actacacacc
      361 ttatgatgga gacgaaagct tccttgcagg accaacagag cgttcacttc acatcaagaa
      421 aattgtagaa gaaactaaag cacactacga agaaactcgt ttcccaatgg acactcgtcc
      481 aacatctatc gctgatatcc ctgctggatt tatcgacaaa gaaaatgaag ttatcttcgg
      541 tatccaaaac gatgaactct tcaaattgaa cttcatgcca aaaggtggta tccgtatggc
      601 tgaaactact ttgaaagaaa atggatacga accagaccca gctgttcacg aaatcttcac
      661 taaatatgta acaacagtta acgacggtat tttccgtgcc tacacttcaa atattcgtcg
      721 cgctcgtcac gcacacactg taactggtct tccagatgca tactcacgcg gacgtatcat
      781 cggtgtttac gcacgtcttg ctctttacgg tgcagactac ttgatgcaag aaaaagtaaa
      841 tgactggaat gcaatcaaag aaatcgatga agaaacaatc cgtcttcgtg aagaagtaaa
      901 ccttcaatac caagcattgc aacaagttgt tcgcctgggt gacctttacg gggttgatgt
      961 tcgcaaacca gcgatgaacg tgaaagaagc aatccaatgg gttaacattg ctttcatggc
     1021 tgtctgccgt gtgattaacg gtgctgctac atctctaggt cgtgtaccaa tcgtattgga
     1081 catctttgca gaacgtgacc ttgctcgtgg tacatttact gaatcagaaa tccaagaatt
     1141 cgttgatgat ttcgttatga aacttcgtac agttaaattt gctcgtacaa aagcttatga
     1201 ccaattgtac tcaggtgacc caacctttat cacaacttct atggctggta tgggtaacga
     1261 cggtcgtcac cgtgttacta agatggacta ccgtttcttg aacactcttg acaacatcgg
     1321 taactcacca gaaccaaact tgacagttct ttggactgac aaattgccat acaacttccg
     1381 tcgctactgt atgcacatga gccacaaaca ctcttctatc caatacgaag gtgtaacaac
     1441 aatggctaaa gacggatatg gtgaaatgag ctgtatctca tgctgtgtgt ctccacttga
     1501 tccagaaaat gaagaacaac gccacaacat ccagtacttc ggtgctcgtg taaacgttct
     1561 taaagccctt cttactggtt tgaatggtgg ttacgacgat gttcacaaag actacaaagt
     1621 atttgatatc gaaccaatcc gtgacgaagt tcttgaattt gaatcagtta aatcgaactt
     1681 tgaaaaatct cttgactggt tgactgacac ttacgtagat gccttgaaca tcatccacta
     1741 catgactgat aggtacaact acgaagctgt tcaaatggcc ttcttgccaa ctaaacaacg
     1801 tgccaacatg ggattcggta tctgtggatt tgctaacact gttgatacat tgtcagctat
     1861 caaatacgct acagttaaac caatccgtga cgaagatggc tacatctacg attacgaaac
     1921 aatcggtgac tacccacgct ggggtgaaga tgacccacgt tcaaacgaat tggcagaatg
     1981 gttgatcgaa gcttacacaa ctcgtctacg tagccacaaa ctatacaaag acgcagaagc
     2041 tacagtatca cttttgacaa tcacatctaa cgttgcttac tctaaacaaa ctggtaactc
     2101 accagttcac aaaggtgtat acctcaacga agatggttct gtgaacttgt ctaaacttga
     2161 attcttctca ccaggtgcta acccatctaa caaagctaaa ggtggttggt tgcaaaactt
     2221 gaactcactt tctagccttg actttagtta tgcagctgac ggtatctcat tgactacaca
     2281 agtatcacct cgcgctcttg gtaagactcg tgatgaacaa gttgataact tggtaacaat
     2341 ccttgatggt tacttcgaaa acggtggaca acacgttaac ttgaacgtta tggacttgaa
     2401 cgatgtttac gaaaaaatca tgtcaggcga agacgttatc gtacgtatct ctggatactg
     2461 tgtaaacact aaatacctca ctccagaaca aaaaactgaa ttgacacaac gtgtcttcca
     2521 cgaagttctt tcaatggatg acgccttgga tgcattgagc taatcaagtt cttgaataat
     2581 aaaaaggaac cctcggtcaa acgactgagg gttttgtgct tgggatagta tgagcaattc
     2641 cttcggcgca atatgcaatg ttgtt
//
LOCUS       DAVBCG010000171         2640 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVBCG010000171 DAVBCG010000000
VERSION     DAVBCG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.171"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(563..1432)
                     /locus_tag="VQ495_001961"
     CDS             complement(563..1432)
                     /locus_tag="VQ495_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2531876.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(1655..2278)
                     /locus_tag="VQ495_001962"
     CDS             complement(1655..2278)
                     /locus_tag="VQ495_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2531877.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDKPTPEPGTPKAETPVNPDPE
                     VPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFFKKKEDEK"
     gene            complement(2236..>2640)
                     /locus_tag="VQ495_001963"
     CDS             complement(2236..>2640)
                     /locus_tag="VQ495_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2531878.1"
                     /translation="TFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYK
                     EGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANA
                     YQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
BASE COUNT          735 a          551 c          508 g          846 t
ORIGIN      
        1 aacctcaaaa ctgtgttttg aacaacctgc ggctagcttc ttagtttgcc ctttgatttt
       61 cattgagtat aaaattgcag ataaaacgat aacaatcata ggactcagca gagtcaatgt
      121 aggtcgaaga atagcccccc taatcgctta ttttatagca aaatgaaata gaaactgaac
      181 attttgttga ggaaggtaaa tcaattcctc acaatgtttg agaaagaagg gaggactatt
      241 ccaacttcaa cccactataa agcagactta caagataggg aacccatctc cgataatata
      301 tagtagaatg aaaactgcac aatttgattg gagaattcaa accaatttct aacaatgttt
      361 tagaaacaag ggtctattat ttcaactgca acctactata gcccattgaa gctggaatag
      421 cccactgaga cttctaaacc actgctggag aggagtttgt ttagagtttc tttcatttcc
      481 ctaaaaaatc aaaaagagag aaccagtctg atcctctctt acttatagat ggtgctcact
      541 caaatgttaa aggagctatt tcttagagat aaaggaattt gaatacagct actgccgcaa
      601 ttgctgctgc gataggtgct actactggta cccaagaata ccaccatttt gaatcgcctt
      661 tatgctcacc aagaactgat tttggaagga aagcatgaag gagacgtggt cccaagtcac
      721 gggctgggtt caaggcaggt cctgtaggtc ctccaagtga tgttaccaaa gccataacga
      781 ggaatccaag tgccaagtga gccacagaaa gtcctgaagc agtgtgtgga gccacctgtg
      841 ctttaatagc caagtcagaa aaatcaactg tttgtcctgc ttctattgcc ttttgtttca
      901 tgaattgaag cacttcagca ccaaagaagt ttttagtcaa accaagagct gcaaagaaca
      961 aaacaaatga accaacaaac tcattaatca aaccattgac agttgctgca tagcgacttt
     1021 cttttgtacc atggtcaata cttgaaatag ttgagaaagt tcccaagatg ttatttggtt
     1081 tttcagtttt caagtagaat ggacggtatg ttgccacaac taaggcttgg ccaaagatag
     1141 cccccaagac ttgcgcgata atgtaaggta ccacttgtgc ccaagggaaa agaccgctaa
     1201 ctgcaagccc tagagtgaaa gcagggttga tgtgattccc agatacgtta ccaaacatca
     1261 aggctgggat cataaccccc ataccataac caacagcgat gacgatccag ccactttggt
     1321 gacctttcgt acctttaagt tcaacgttgg caactgcacc attcccaaga atgatcaaaa
     1381 tggcagttcc caaaaattca gtggcatact tcagtgccca tgtgaaatcc atttgctaga
     1441 ttctccttaa aattttttag atattcctta ttttatcaat ttttacccta tttagcaact
     1501 gatttctgga aatattttta aaaaaatagg attcatagtt tcccagttca acgtacttgt
     1561 aagcgttttt attcttcacg aaagacctca ttctcaaaac caggaaaaag cccacatctg
     1621 tgatcttttc ctggttccta gattctaaaa tctattattt ttcatcttct tttttcttga
     1681 agaatcctaa tcctagacca gctaacaagg tcatgatacc agcactagcc aaggtagcat
     1741 tagcttctgt acctgtgttt ggcaattcct ctctcttacc tgtctcataa gtcggaactt
     1801 ctgggtctgg attcactgga gtttcagctt ttggagtacc tggttctgga gttggtttat
     1861 ctggtgttgg agtctcttga tttggtagtt ctgggtttgg tgtcggaaca cttggatgct
     1921 ctggatttgg ttgtgggatt ggtttttctg gagttggttt atctggtgtt gataaacggt
     1981 cataccttac cgttatttct ttatcactag agtctgacgt aacttcttgt gattcaactg
     2041 ttggaatatc tggatctttg tacttgtcaa tcttaccaga tataacctcg tcccagtttc
     2101 ctgttgtcca ttcaccgtag gttacaactc ccgtgacctt gttctcagtt tttgtacggc
     2161 ttaaggttac aggttgaaca acatcttctt ttacattttg gttcgtaact ttatcaacgt
     2221 aatgaatgat acgcgttata gtcttcgtct cagtagaggt tgctgttttg ggaaccactg
     2281 tttcctcaac attctcacgg tagtaatagt caactgttgc accgtcttct ggtacgcatt
     2341 tgcaggagtt gctaccaagg tgtatgttgt tttccttgtg ataactcggt cttctttgtc
     2401 ctcagttgtt gttttccctt caatagtttt tgattctgtg gtatactcag aacctatcgc
     2461 taaatcagct tttataacag actctgccaa cttctcttgg ctaccttctt tatagtaatt
     2521 ggctgttact gtagcagtgg ttggcgcttc gctttctcta taaactaagg tcactgttct
     2581 accttcgctt acaatattcc cagttaaact tgcagaattt gtatctgctt ctttaaaagt
//
LOCUS       DAVBCG010000172         2603 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVBCG010000172 DAVBCG010000000
VERSION     DAVBCG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.172"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            410..2515
                     /locus_tag="VQ495_001964"
     CDS             410..2515
                     /locus_tag="VQ495_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2531879.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTSEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
BASE COUNT          839 a          469 c          605 g          690 t
ORIGIN      
        1 tatagttttg agatggttga tagaactgac gaagtcagct caaaacatgg ttttgaggtt
       61 gtagataagg agaagctgat gtggtttgaa gaggttttcg aagagtgtaa aaaaatattg
      121 gtcagttaaa gccaaaactc ttgacctttt ctgaccaatg tagtatatta tgaacgtaag
      181 gtaaatgaaa gccttactag aacacttatt taaagtaaaa tagaaagagg tgggtctatg
      241 tttcatctag aaatcttcag aagtaaagat ggtctactcc tgcttgaaaa agaaaaacca
      301 gaaatagtac gtagggtagc ggtttaacta gtctaaattt tttaaaacaa tggtcaaaga
      361 tagtcaatat cagtaatcat aactaagtaa acaaaaagag gtaaagaata tgaataacaa
      421 ctttaataat tttaataaca tggatgattt atttaaccaa ttgatgggtg gtatgcgagg
      481 atatagttct gaaaatcgcc gttacttaat taatggacgc gaagtcacat ctgaggaatt
      541 tgctcactat cgtgcgactg gtcaattacc aggaaatgca gaaactgatg tgcaaatgcc
      601 acaacaggca tcaggtatga aacaagacgg tgtccttgca aaactaggtc gaaacttgac
      661 agcagaagcg cgtgagggca agttggatcc tgttatcgga cgaaacaagg aaattcaaga
      721 aacatctgaa atcctctcac gccgcaccaa gaacaatcct gttttggtcg gagatgcagg
      781 tgttggtaag acagcagttg tcgaaggtct agcgcaagcc attgtgaacg gagatgttcc
      841 tgctgctatc aagaacaagg aaattatttc tattgatatc tcaggtcttg aggctggtac
      901 tcaataccgt ggtagctttg aagaaaatgt ccaaaactta gtcaatgaag tgaaagaagc
      961 agggaatatt atcctcttct ttgatgaaat tcaccaaatt cttggtgctg gtagcactgg
     1021 tggagacagt ggttctaaag gacttgcgga tattctcaag ccagctctct ctcgtggaga
     1081 attgacagtg attggggcaa caactcaaga cgaataccgt aacaccatct tgaagaatgc
     1141 tgctcttgct cgtcgtttca acgaagtgaa ggtcaatgct ccttcagcag agaatacttt
     1201 taaaattctt caaggcattc gtgacctcta tcaacaacat cacaatgtca tcttgccaga
     1261 cgaagtcttg aaagcagcgg tggattattc tgttcaatac attcctcaac gtagcttgcc
     1321 agataaggct attgaccttg tcgatgtaac agctgctcac ttggcggctc aacatccagt
     1381 aacagatgtg catgctgttg aacgagaaat cgaaacggaa aaagacaagc aagaaaaagc
     1441 agttgaagca gaagattttg aagcagctct aaactataaa acacgcattg cagaattgga
     1501 aaagaaaatc gaaaaccaca cagaagatat gaaagtgact gcaagtgtca acgatgtggc
     1561 tgaatctgtg gaacgaatga caggtatccc agtatcgcaa atgggagctt cagatatcga
     1621 acgtttgaaa gatatggctc atcgcttgca agacaaggtg attggtcaag ataaggccgt
     1681 agaagctgta gctcgtgcta tccgtcgtaa ccgtgctggt tttgatgaag gaaatcgccc
     1741 aatcggcagc ttcctctttg tagggtctac tggggttggt aagacggagc ttgctaagca
     1801 attggcactc gatatgtttg gaacccagga tgcgattatc cgtttggata tgtctgaata
     1861 cagtgaccgc acagctgttt ctaagctaat tggtacaaca gcaggctatg tgggttatga
     1921 tgacaatagc aataccttaa cagaacgtgt tcgtcgcaat ccatactcta tcattctctt
     1981 ggatgaaatt gaaaaggctg atcctcaagt tattaccctt ctcctccaag ttctagatga
     2041 tggtcgtttg acagatggtc aaggaaatac agtaaacttc aagaacactg tcattattgc
     2101 gacctcaaat gctggatttg gctatgaagc caacttgaca gaagatgcgg ataaaccaga
     2161 attgatggac cgtttgaaac ccttcttccg tccagaattc ctcaaccgct ttaatgcagt
     2221 catcgagttc tcacaattga ctaaggaaga cctttctaag attgtagatt tgatgttggc
     2281 tgaagttaac caaaccttgg ctaagaaaga cattgacttg gtagtcagtc aagcggctaa
     2341 agattatatc acagaagaag gttacgacga agtcatgggg gttcgtcctc tccgtcgcgt
     2401 ggttgaacaa gaaattcgtg ataaggtgac agacttccac ttggatcatt tagatgctaa
     2461 acatctggaa gcagatatga aagatggcgt tttggttatt cgtgagaaag cctaagacag
     2521 aattttgagg ataaaaaaga aggagccagc tgaaaaaact ggttcctttt gaggtacgac
     2581 gggcatgtcg tatagtagaa gtg
//
LOCUS       DAVBCG010000173         2560 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVBCG010000173 DAVBCG010000000
VERSION     DAVBCG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.173"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            176..490
                     /locus_tag="VQ495_001965"
     CDS             176..490
                     /locus_tag="VQ495_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531880.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            500..706
                     /locus_tag="VQ495_001966"
     CDS             500..706
                     /locus_tag="VQ495_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2531881.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            706..903
                     /locus_tag="VQ495_001967"
     CDS             706..903
                     /locus_tag="VQ495_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531882.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            906..1628
                     /locus_tag="VQ495_001968"
     CDS             906..1628
                     /locus_tag="VQ495_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2531883.1"
                     /translation="MKKKRRLLFLMSIVLGMFVGMFKARVESHEIILDVKALMPWISA
                     ICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITG
                     VAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFAT
                     PKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQLLAFL
                     LVGAIHVYINVMQLPMVKRYFK"
     gene            1640..2281
                     /locus_tag="VQ495_001969"
     CDS             1640..2281
                     /locus_tag="VQ495_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2531884.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2299..2529
                     /locus_tag="VQ495_001970"
     CDS             2299..2529
                     /locus_tag="VQ495_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531885.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          753 a          422 c          515 g          870 t
ORIGIN      
        1 aagagatttt cgaagagtat taatgataga taaactggtc agtaacctac tcctgacctt
       61 tttcttttgc aaaatgacaa aaatcataaa ctttttgaca actatacttg tcaaaaagaa
      121 aaagatgtgt tacaatgaat tcaagttaag aaataggaag cagaaaggag ttataatgtg
      181 ggtactagta tttatactat ttatgatttt cttttattct aataattcta aaaaaatcaa
      241 gaaactagag aataaaatca aaaaacttga gcgaaaagag aaaggaaacg cagaaatgtc
      301 gagattattg caagaaatga ttggaaagga accaattata acgggagtgt atattgggcc
      361 agataactgg gaagttgtgg atgttgatga ggaatgggta aagctacgac gtgtagataa
      421 tacgggaaaa gaaaaattca agttgcaacg cattgaggat atccaaaccg ttgaatttga
      481 cggagagtag gtgtgtaaca tgcaacttaa aaatcgtcta aaagagctta gagctcgcga
      541 tggtctcaat caaaccgacc tagccaaact ggcaggggtt tccagacaga ccattagcct
      601 actagaacgg gatgagtaca ccccatccat tatcattgcc ctgaaaatat ctcaaatttt
      661 caacgaaaca gtcgaatcgg tatttcgttt ggaggaggac gagtgatgaa caagtataaa
      721 gtgatttatt atgtagtggt catagcttta ttagtcagtg tatttctact gattgggatg
      781 gacctaagct ggtttaatcc ctatcaaagc gaccaatttg tttgggtcta ctttgctctc
      841 atcccagtaa ttgaatggat tgaaaagaaa tccaaaaatc tagcaagtga aaaaggagaa
      901 tgaatatgaa aaagaaacgt agattgttat ttttgatgtc tattgtcttg ggcatgtttg
      961 tagggatgtt taaggcacgt gtcgaatccc acgaaattat tttagatgta aaagccttga
     1021 tgccatggat atcagctatt tgtttactga taggtttcat tagtatgttt ttgactttca
     1081 atttcttaaa gaaaagcaga aaatttcatt ccttgtatca agaggaaatg gatgacgatc
     1141 tgaatgaaac ctattatgtg caaatgtatc ggaatcttga gtttggaacc attgctttta
     1201 atattacagg tgtagcgatt ccattggcta tttttatttc attaagtgag gtgattatat
     1261 tgcatacaaa ccctcaaaca tttttccttt ctttcttact ctttgtggta ttcttagtcg
     1321 ctcaaaaatc tctttttaaa accattgcga ttgttcgtca gtttgattta gaatttttcg
     1381 ctacaccaaa ggatgtcttg aactatataa attcttatga tgaaggggag cgtcaggcta
     1441 atttggaaca gagttttcga attttattcc aattacacca atatgtctta ccagccttat
     1501 atatttttct tattatcatt tctttcttga caggagagat tcagttacta gctttcttgc
     1561 ttgtaggagc catccatgtt tatatcaatg tgatgcagtt acctatggta aaacgttatt
     1621 tcaaataaag gagttatcta tgaaattact taaaaacctt ggctggattc ttctagccct
     1681 tctatccttt ttatttatct atggctttat tcaggggctc gcgactgcgt cgcttgcttt
     1741 aggcgcttca ccctatgctg ttactctact ctatgtggcc ttggctggag tatatgtgta
     1801 cggcatttac aaatggtatc agaaggctcc tgttcatatt gagaagagcg gctttaatcg
     1861 atttatttgg cttcctgtct tggtttggtt cttatctctg gtcgttcaat tcttcttgcc
     1921 agatgatcct tcagtaaatc agcaaatagc gacagacttg accttgtctc aaccactttt
     1981 ctcattcttt gctgtggtta tttttgctcc tttgacggaa gagattgttt ttagagggat
     2041 gttagcacgc tatctctttc ctaagcagga caatagtaaa cgaaccctga tttttcttct
     2101 ggtatctagt cttctatttg ccttgattca ttttccaggt gatgtgcaac aattttttgt
     2161 ctattttagc cttggtttta gtttgggttt ggcttacatt agcagaaaag gtctggtcta
     2221 cagtatttct cttcacgctt tgaataattt agtcggcttt ttgatgattc tcatgctata
     2281 atagagtcag gaggtcacat gaaacgagta attttattag cagtgattca ggcagtcgtt
     2341 ctatttttca tcattggagc gctagcttat gccttcaaag gcgatttctt ctataactat
     2401 ctagcagttg tctttgctcc tattgcaggt gtactgcgtt ttgggacggc ttacataacg
     2461 gaaattgtct tgcctcgaaa ggcagccgaa atcgctgaaa agcgtaaagc aggcaaaaat
     2521 tcaaaataaa agagtccggt gaagttcatc ggatttttgt
//
LOCUS       DAVBCG010000174         2541 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVBCG010000174 DAVBCG010000000
VERSION     DAVBCG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.174"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            76..993
                     /locus_tag="VQ495_001971"
                     /pseudo
     CDS             76..993
                     /locus_tag="VQ495_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(1033..1977)
                     /gene="manA"
                     /locus_tag="VQ495_001972"
     CDS             complement(1033..1977)
                     /gene="manA"
                     /locus_tag="VQ495_001972"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2531886.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2109..2390)
                     /locus_tag="VQ495_001973"
     CDS             complement(2109..2390)
                     /locus_tag="VQ495_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2531887.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
BASE COUNT          677 a          591 c          465 g          808 t
ORIGIN      
        1 gtgcatcagt tagttgttta cttgcttcat cattcataga actactatgc catattttgt
       61 ttcgcaggaa gtctgatcat ttcaaatcca gccattgctc taggagctca agcgaccttt
      121 atcctattga atatcttcat tgtatcacgt ggggttcaaa aagggattga aagagcttcg
      181 aaagtcatga tgcccctgct ctttatcatc tttgtcgtca tcatcgaacg ctctctcagt
      241 ttgccaaatg ccatggaagg ggttctttac ttcctcaaac cagatttttc aaaactgact
      301 agcgctggtc tcctctatgc tctgagacaa tctttctttg ccctctcact aggggttaca
      361 gccatgttga cctatgcttc ttacttggac aagaaaacca atctggtcca gtcaagaatc
      421 tccatcgtag ccatgaatat ctcggtatcc atcatggcag gtctagccat tttccaagct
      481 cggtccccct tcagactgaa tattgaaggg ggaccgagct tggtctttat cgtcttgcct
      541 caactctttg acaagatgcc ttttggaacc attttctatg tcctcttcct ctttgcgact
      601 gtcacttctt ctgtcgttat gctggaaatc aatgtagaca atatcaccaa tcaggacaac
      661 agcaagcgtg ctaaatggag tgttatttta ggaattttga cctttgtctt tggcattcct
      721 tcagccctat cttacggtgt catggcggat gttcacattt ttggtaaagc cttctttgac
      781 gctatggact tcttggtttc caatctcctc atgccatttg gagctctctt cctttcactt
      841 tttacaggct atatctttaa aaaggctctt gcaatggagg aactccatct cgatgaaaga
      901 gcatggaaac aaggactgtt ccaagtctgg ctcttccttc ttcgtttcgt cattccaatc
      961 atcatcgtgg tcttcatcgc ccaatttatg taatcaaaag gacttgagta atgaactcag
     1021 gtcctttctt ttttatggat gactaacaat taattctaaa tcttgccctt ctagagtcca
     1081 agattcaaca tcacttggta ggataaagtg tctccctttt tggattggat agtttttccc
     1141 gtcaacagtt agttgacctt gaccagccaa gacactcaac aagctgtagt cagctgtctt
     1201 ttcaaagtta acttttccag taatttccca tttgtaaact gcgaagaaat cattggatac
     1261 aaggatagtg gaacgtagat catctgcttt gattgtcact ggacggctat ttgcgggttc
     1321 accaatgttc aagacatcga tagatttttc aaggtgaagt tcacgtaaat tgcccttgtc
     1381 atccttacgg tcaaagtcat agacacggta agtggtatcg ctagactgct gggtttcaag
     1441 aatcaagata cccgccccga tagcgtgcat ggtaccgctt ggtacataga agaaatctcc
     1501 tgctttaact ggcacttttg tcaacagcgc atcccagttc ttatcctcga tttgctggcg
     1561 gagttcttct tttgacttgg cattgtgacc gtagataatc tctgaacctt catctgctgc
     1621 aatgatgtac caacattctg tttttccaag ttcgccttca tgctcgagtc cataagcatc
     1681 gtctgggtga acttggacac tgagccagtc gttggcatcg aggatcttgg tcaaaagtgg
     1741 aaatacaggt tctggacgat tgccaaataa ttcacggtgt tccgcataca aagtagcaag
     1801 atctgttccc tcgtaacgac cattggcaac tttagaaaca ccatttagat gagctgagat
     1861 agcccagtac tctccgactt tttcacttgg gatgtcgtag ccaaactcat cacgtagctt
     1921 ggtaccaccc cagatttttt cttgcataac tgattgtaaa aataatggtt ctgacatctt
     1981 aatctcctgt ctgatttttc tcccctcatt atagcaaaaa aagagttcca tttgaactct
     2041 tttttacatc ttataaagca gggagaagat tttataaaaa tagtaaacaa atgtgctcta
     2101 ctcgatgctt acaccattgc tataaatgac atgtaccaat agaaggactt cttcttgcta
     2161 cgtttgagag tcccatttcc tacattatct cgatctacat agataaagcc atagcgctta
     2221 ttcatttccc ctgtgccagc tgaaaccgaa tcgatacagc cccatgtcgt ataaccaagc
     2281 aagtcaaccc cgtctttgta aatggcatct cgcatggcct tgatgtgggc ctctaagtaa
     2341 gtaatccgat agtcatctgc tacataacca ttctcatccg gtgtatccat agcaccgagt
     2401 ccattttcta cgataatact atactaaaat caaaaagcat tatataatag tgatatgaaa
     2461 tcaactaaag aagaaatcca aaccatcaaa acacttttta aagactctcg tacagctaaa
     2521 tatcataaat gccttcaaat c
//
LOCUS       DAVBCG010000175         2473 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVBCG010000175 DAVBCG010000000
VERSION     DAVBCG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.175"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..1545
                     /gene="phtA"
                     /locus_tag="VQ495_001974"
     CDS             <1..1545
                     /gene="phtA"
                     /locus_tag="VQ495_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2531888.1"
                     /translation="HVESDGLVFDPAQITSRTARGVAVPHGDHYHFIPYSQMSELEER
                     IARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSNSSLVSQLVRKVG
                     EGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQEFYDK
                     AYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPITHPER
                     LGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSHWIGK
                     DSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLVRLPY
                     MVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVEHPDE
                     RPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVEAQLK
                     EAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSNPSSV
                     SKEKIN"
     gene            1703..>2473
                     /locus_tag="VQ495_001975"
     CDS             1703..>2473
                     /locus_tag="VQ495_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2531889.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNW"
BASE COUNT          868 a          454 c          501 g          650 t
ORIGIN      
        1 catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg aacagctaga
       61 ggtgttgcag tgccacacgg agatcattac cacttcatcc cttactctca aatgtctgaa
      121 ttggaagaac gaatcgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca
      181 gattcaaggc cagaacaacc aagtccacaa ccgactccgg aacctagtcc aggcccgcaa
      241 cctgcaccaa atcttaaaat agactcaaat tcttctttgg ttagtcagct ggtacgaaaa
      301 gttggggaag gatatgtatt cgaagaaaag ggcatctctc gttatgtctt tgcgaaagat
      361 ttaccatctg aaactgttaa aaatcttgaa agtaagttat caaaacaaga gagtgtttca
      421 cacactttaa ctgctaaaaa agaaaatgtt gctcctcgtg accaagaatt ttatgataaa
      481 gcatataatc tgttaactga ggctcataaa gccttgtttg aaaataaggg tcgtaattct
      541 gatttccaag ccttagacaa attattagaa cgcttgaatg atgaatcgac taataaagaa
      601 aaattggtag atgatttatt ggcattccta gcaccaatta cccatccaga gcgacttggc
      661 aagccaaatt ctcaaattga gtatactgaa gacgaagttc gtattgctca attagctgat
      721 aagtatacaa cgtcagatgg ttacattttt gatgaacatg atataatcag tgatgaagga
      781 gatgcatatg taacgcctca tatgggccat agtcactgga ttggaaaaga tagcctttct
      841 gataaggaaa aagttgcagc tcaagcctat actaaagaaa aaggtatcct acctccatct
      901 ccagacgcag atgttaaagc aaatccaact ggagatagtg cagcagctat ttacaatcgt
      961 gtgaaagggg aaaaacgaat tccactcgtt cgacttccat atatggttga gcatacagtt
     1021 gaggttaaaa acggtaattt gattattcct cataaggatc attaccataa tattaaattt
     1081 gcttggtttg atgatcacac atacaaagct ccaaatggct ataccttgga agatttgttt
     1141 gcgacgatta agtactacgt agaacaccct gacgaacgtc cacattctaa tgatggatgg
     1201 ggcaatgcca gtgagcatgt gttaggcaag aaagaccaca gtgaagatcc aaataagaac
     1261 ttcaaagcgg atgaagagcc agtagaggaa acacctgctg agccagaagt ccctcaagta
     1321 gagactgaaa aagtagaagc ccaactcaaa gaagcagaag ttttgcttgc gaaagtaacg
     1381 gattctagtc tgaaagccaa tgcaacagaa actctagctg gtttacgaaa taatttgact
     1441 cttcaaatta tggataacaa tagtatcatg gcagaagcag aaaaattact tgcgttgtta
     1501 aaaggaagta atccttcatc tgtaagtaag gaaaaaataa actaatgaaa aatgaaagtc
     1561 tcgataaaga ggctttcatt tttattatgt atatatgtaa aattcttgac aagcaatatt
     1621 aaaaagagta aactattaac tagttaatta accggtttat tactttatag tgaatcaaat
     1681 atacttaaga aaagaggaaa gaatgaaaat caataaaaaa tatctagctg ggtcagtagc
     1741 tacacttgtt ttaagtgtct gtgcttatga actaggtttg catcaagctc aaactgtaaa
     1801 agaaaataat cgtgtttcct atatagatgg aaaacaagcg acgcaaaaaa cggagaattt
     1861 gactcctgat gaggttagca agcgtgaagg aatcaatgcc gaacaaatcg tcatcaagat
     1921 aacagaccaa ggctatgtca cttcacatgg ggaccactat cattattaca atggtaaggt
     1981 tccttatgac gctatcatca gtgaagaatt gctgatgaag gatccaaact accagctaaa
     2041 ggacgaggat attatcagtg aaatcaaggg tggttatgtg attaaggtag atggaaaata
     2101 ctatgtttac cttaaggatg cagctcatgc ggataatgtc cgtacaaaag aagaaatcaa
     2161 tcggcaaaaa caagaacata gtcagcatcg tgaaggaggg acttcaacaa acgatggtgc
     2221 ggtagccttt gcacgttcac aaggacgcta tactacagat gatggttata tctttaatgc
     2281 ttctgatatc attgaggaca cgggtgatgc ttatatcgtt cctcacggcg accattacca
     2341 ttacattcct aagaatgagt tatcagctag cgagttagct gctgcagaag ccttcctatc
     2401 tggtcgggga aatctgtcaa attcaagaac ctatcgccga caaaatagcg ataacacttc
     2461 aagaacaaac tgg
//
LOCUS       DAVBCG010000176         2328 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVBCG010000176 DAVBCG010000000
VERSION     DAVBCG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.176"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            121..798
                     /locus_tag="VQ495_001976"
     CDS             121..798
                     /locus_tag="VQ495_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HET2531890.1"
                     /translation="MYRGTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIH
                     LVNDAEINVFNGDLGAITDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAM
                     SIHKSQGSEFPVVILPITSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIG
                     TARKTYLIERFSDLLENVEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTD
                     LKEIFGK"
     gene            complement(846..2129)
                     /locus_tag="VQ495_001977"
     CDS             complement(846..2129)
                     /locus_tag="VQ495_001977"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2531891.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     ATAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT          641 a          506 c          438 g          743 t
ORIGIN      
        1 aaagctgacc gttcctactt tgaaattgct agtggccata ttcctgccac cattgaaaaa
       61 atcttaggtg ccgccctcag aaatggtatt cctgcccgtg atatccaagt tctggctcct
      121 atgtaccgag ggacggcagg gattgatgct atcaatcagc tcatgcaaga cctgctcaat
      181 cccccacaaa aagatcaact cagttttgaa gctcctcagt gccactatcg taagggagac
      241 aaggtcattc atttggttaa cgatgctgaa atcaatgtct ttaatgggga tttaggagct
      301 atcacagacc tgattcctgg taaatacacc gaatcgaaac aagacgagat tgtcattgat
      361 tttgatggta atgaagtctc ttacccccgt aacgaatggt acaagattcg cttggcctat
      421 gccatgagta ttcataagtc tcagggaagt gagttcccag ttgtcatcct accgattact
      481 agtgctagcc gacgtatgct ggagcgaaat ctcatctaca cagccattac acgtgccaaa
      541 agcaaactca tcttactagg cgaattacaa gccttcgact atgctaccca acacatcgga
      601 actgcccgaa aaacctatct gattgaacgc ttcagtgatc tactggagaa tgttgaagaa
      661 aagcaacaag ctgtctctga aacagtcaca tcaagtgcct ctgaacaatc ctacatccta
      721 accgaagaaa actgggacag cattccagcc atgattggga ttacagacac agacctcaaa
      781 gagatttttg gaaaatagtg catcagaaaa accaccaatt caggtgggtt ttctgtttat
      841 taagattatt ttactgttgc tgtgcttgta atcaattcaa cagctttttt gatagtgata
      901 tcatgtttca acatgtcagc tgaaagcaag ttttgaactt gtgcaacttc catgttgtag
      961 tctgctgcca attgctcaac ttctttttgg atttcttctt ctgaagcatc aaatccttca
     1021 gctttggcaa ctgcttcgat aacaaggtta gtcttagtac gtgactcagc ttctgcttgg
     1081 tattggttgt gaaggtcttc ttgagtagtt ccagtgattt ggaagtacat gtcagggttg
     1141 atcccttgac gttgcaagtt cccaaggaat tcatttactg aacggtgaac ttcttcgtgg
     1201 atcatttctt ctggaagttc tacgatttca gcattttcta cagctgtatc aattgctgca
     1261 ccttcaactg catctttgta agcttcttct ttagcagtag ccaattcttt gcggtatttt
     1321 tctttcaagt cagcaagtgt ttcaacttct tcatcaatgt cttttgcaag ttcatcgtca
     1381 agagctggaa cttctttagc ttttacttcg tggatagttg tcacgaattt agcttcttta
     1441 cctgcaaggt cttctgcttg gtagtcttct gggaatgtta cgataacatc aacggtttcg
     1501 ccagctgagt gacctaccaa ttggtcttcg aaaccaggga tgaattgacc tgaaccaagt
     1561 ccaagtgaga agttttcacc ttttccaccg tcaaattcaa caccgtcgat agaaccaacg
     1621 aagtcgatca caacagtgtc gccgttttca gcagcagctt ccttgataac caattcagcc
     1681 agattgttgc gttcgcgttc gatacgctct tcaacatcag cgtcagttac ttctttttct
     1741 acatcaactg atacttcaag gtttttgtag tcacccaatt ttacttcagg ttttgtaacg
     1801 acttcagcag tgataaccca gtcttgacct ttttccattg aagttacgtc aatttttggt
     1861 tgggcaacca cttcaagacc agcttctttt acagctgctt cataagcgtt tggcaaaaga
     1921 gcgttcataa cgtcttggta aagtgattct tcaccaaatt ttttatcgaa gataggacgt
     1981 ggaaggtgac ctttacggaa acctggaaca ttaagagatt tcttcactga cttgaagaca
     2041 cggtccaatt ctggtttgat ttggtcttga gagatagtga aagtcaagac accacggttt
     2101 gtttctttgt tttcaaatga tacagacatt ctgtcatttc tccttaaaat tttttaaata
     2161 cagtctatta taacattttc tataaataaa atctagacta atgcagccaa ttatagacaa
     2221 caagcctttt tcctaactaa aacactcata aagtatagac aattttagac tactttggat
     2281 atcttttggt tagtcatttt ccaaaactaa tttatctaat ttttcagt
//
LOCUS       DAVBCG010000177         2217 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVBCG010000177 DAVBCG010000000
VERSION     DAVBCG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.177"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQ495_001978"
     CDS             101..1078
                     /locus_tag="VQ495_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2531892.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYKTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            1075..2157
                     /locus_tag="VQ495_001979"
     CDS             1075..2157
                     /locus_tag="VQ495_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2531893.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
BASE COUNT          701 a          500 c          402 g          614 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt ctcgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattacc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt gttggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacaa aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcattaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagcccctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagaaaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgacca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattgactg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggccgtatg
     2041 ggcaaggaag gatatgttat tactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aatttta
//
LOCUS       DAVBCG010000178         2115 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVBCG010000178 DAVBCG010000000
VERSION     DAVBCG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.178"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..91)
                     /locus_tag="VQ495_001980"
     CDS             complement(<1..91)
                     /locus_tag="VQ495_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2531894.1"
                     /translation="MNYEASKQLTDTRFKLLVGVQRTTFKEMLA"
     gene            216..674
                     /locus_tag="VQ495_001981"
     CDS             216..674
                     /locus_tag="VQ495_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2531895.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            687..1295
                     /locus_tag="VQ495_001982"
     CDS             687..1295
                     /locus_tag="VQ495_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531896.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1366..1698
                     /locus_tag="VQ495_001983"
     CDS             1366..1698
                     /locus_tag="VQ495_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531897.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1709..>2115
                     /gene="ply"
                     /locus_tag="VQ495_001984"
     CDS             1709..>2115
                     /gene="ply"
                     /locus_tag="VQ495_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2531898.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWH"
BASE COUNT          657 a          387 c          457 g          614 t
ORIGIN      
        1 cagctaacat ctctttaaaa gtggtacgct gaacaccaac aaggagctta aatcgtgtat
       61 cagttaattg tttacttgct tcataattca tagaactact ataccatatt ttgtttcgca
      121 ggaagtctaa taagttatag tccagttttt actatacttt gatattcaaa tatgttatac
      181 tatgattatc aaagtaaatg aaaggagtta cgaatatgac gccagaagaa atgtacctga
      241 cagagcgatt agacgtacag atagctcatt ttttaaagaa aagcgttcaa catcgtaggc
      301 gctataaggt attaaaaata acagaaatcg tggcaggttt cctcatagct gtcttttgtg
      361 ctattcctat gccaggtgat cgctaccgtt tgatttcggt tgccttatcc agtctcggct
      421 tgctgtgtga ggggattatc aatttgtata atgcaaagga aaattggatt tcttaccaaa
      481 aaactgcgca actcctggag aaagaaaaat tcctctatca atgccaaacg gagaaatatg
      541 caggaaagac caaggctttt gccctatttg tcaagacatg cgaaggtctt atctcagagg
      601 agattaacca gtgggaaagt atccagtcaa aagaagtggc agctagtgca gatgctccag
      661 ttaaaaaaga gtaggaggta gaggaaatgt ctcaatccag ctacctgtcg cccttgctct
      721 ggttaaaaaa agaagccgat aaggaaaaga tgagcgcgac ccagtgccag atatttttct
      781 tttactatca aatgtttgag ctcttatttg ctagagaaag cgacatgaaa gacttatgtc
      841 tgggaacgaa aggtttttat ttctcgcagt tagagaaaaa tttgctttct ggagtttccc
      901 gatttctaaa aaacttggag gggaaagtaa ctctcaaggc taaccaagaa gtatcagctc
      961 gcaaagccct ttttctagcc ttgacaacta gccaatcaga ttggcaggag ttagctcctg
     1021 tttttgattt ttatcagact atcgggaggc ttgaaaatcc ttctctcttg agttctcagg
     1081 acagacaaca tctgatgtgg atttaccagt cagctttgga gaaggattat attgtcaagg
     1141 ttattggcga caagcatttt gtattgaaga gacaagatgc tactaaattg acagcgcgcc
     1201 aaactcaaac tttggaaatt ctgagtcaat cagaagactt ggtcaatcct gtctatgtta
     1261 cattaggaga aaagggggtg ctcttgcttg attaagagag gagatgttgt agctctttat
     1321 ttgccttttc cgactattag tagcgatttg gctgtgaaga atcatatgta tatctgtatt
     1381 gacaacagca tgactaaaaa caaagagttg gttaaaaatc agaccttcaa accagctctc
     1441 ttgaccagac gtttggtcaa gaactttatg atagaagagc cagatctagc tcgtaatcct
     1501 tttacaagac caaccttgat tgacttagat aaggtattta tgttggataa tacggtcatt
     1561 ccgacttctt atctagccag acggcgacgc aatgtctcag aagaattgta cgaggaaatt
     1621 ttggattact tagtccaacc acggctgatt tcgctgaaca agtctgagtt tatgcaactc
     1681 aatccaggaa cttattagga ggtagaagat ggcaaataaa gcagtaaatg actttatact
     1741 agctatgaat tacgataaaa agaaactctt gacccatcag ggagaaagta ttgaaaatcg
     1801 tttcatcaaa gagggtaatc agctacccga tgagtttgtt gttatcgaaa gaaagaagcg
     1861 gagcttgtcg acaaatacaa gtgatatttc tgtaacagct accaacgaca gtcgcctcta
     1921 tcctggagca cttctcgtag tggatgagac cttgttagag aataatccca ctcttcttgc
     1981 ggtcgatcgt gctccgatga cttatagtat tgatttgcct ggtttggcaa gtagcgatag
     2041 ctttctccaa gtggaagacc ccagcaattc aagtgttcgc ggagcggtaa acgatttgtt
     2101 ggctaagtgg catca
//
LOCUS       DAVBCG010000179         2113 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVBCG010000179 DAVBCG010000000
VERSION     DAVBCG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.179"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            100..1593
                     /locus_tag="VQ495_001985"
     CDS             100..1593
                     /locus_tag="VQ495_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2531899.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1606..>2113
                     /locus_tag="VQ495_001986"
     CDS             1606..>2113
                     /locus_tag="VQ495_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2531900.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLL"
BASE COUNT          745 a          321 c          417 g          630 t
ORIGIN      
        1 tattcaggtg acgcatagat atagttaatt gaagctttgt ttgaaatctg ataaaataat
       61 gatattacta agttttaaaa actaaagaaa agggaagata tgattacagg cgaattaaaa
      121 aataaaatcg atcagctgtg ggaaattctt tggacagaag gaaacgcaaa tcctttaaca
      181 aatattgaac agttgactta tctcttattt atgaaagatt tggatagtgt cgagcttgga
      241 cgtgaaagtg atgctgaatt tctagggatt ccttatgagg gagtttttcc aaaagataaa
      301 cctgaatacc gttggtcaac ttttaaaaat ataggagatg ctcaggaagt ttatcgttta
      361 atgactcagg agatttttcc gtttattaaa aatctcaagg gggatacaga tgatacagcc
      421 ttttcacgat atatgcgaga agctattttt caaataaata aacctgctac gcttcaaaag
      481 gcaatttcta tcttagatgt ttttccaact aggggattag atgtagattt tgataatgac
      541 aaacaaagta ttactgatat cggagatatc tatgaatatc tgttatcaaa attgtcgacc
      601 gcaggtaaaa atggacagtt ccgtacacct cgtcacatca tcgatatgat ggttgagttg
      661 atgcaaccga ctatcaaaga tatcatctca gatcccgcta tgggttctgc tggcttctta
      721 gtatctgcta gccgttactt aaagcgtaag aaagatgaat gggaaaccaa tacagataat
      781 atcaatcatt ttcataatca gatgtttcat ggaaatgata cggatacgac tatgttgaga
      841 cttggggcga tgaacatgat gctacatgga gtagaaaatc cacaaatcag ttaccttgac
      901 tcgctgtctc aagataatga agaagccgat aaatatactt tggttttagc aaatcctcct
      961 tttaagggct cacttgacta caattcaacc tctaatgacc ttcttgcaac cgtaaaaacc
     1021 aaaaaaacag aattactctt tctttctctt ttcttgcgaa ctttaaaacc aggtggacga
     1081 gcagcagtta tcgtacctga tggtgtcctt tttggttcgt ctaaagctca taaaggaatt
     1141 cgtcaggaaa ttgtagagaa tcataagctt gatgctgtaa tctcaatgcc tagtggtgtg
     1201 ttcaagcctt atgctggagt ttcaactgcc attctcatct ttacaaaaac tggtaatggt
     1261 ggtactgaca aagtctggtt ttacgatatg aaagcggatg gtttaagttt ggatgataag
     1321 cgacaaccga ttagcgacaa tgatattcca gatattatcg aacgctttca tcatcttgaa
     1381 aaagaagcag aacgtcagag aacggatcaa tctttctttg ttccagttgc tgagataaag
     1441 gaaaatgatt atgatttgtc tatcaataaa tataaagaga ttgagtatga aaaagttgag
     1501 tatgaaccaa cagaggtcat cttaaagaaa atcaatgatt tagaaaaaga aattcaagct
     1561 ggcttggctg aattagaaaa attactcaag tagagaggtg gctgtatgaa aaaagtgaag
     1621 ttgggggaag tcttatctct aaaaaaaggc aagaaagcca ctgtacttgc tgaacaaaca
     1681 actctaagcc aacgttatat tcaaatagat gatttaagaa ataataataa tttaaaattc
     1741 actgaaagtt taaatatgac tgaagcactc ccagatgata ttctgatagc atgggatgga
     1801 gctaatgcag gaacagttgg ttatggatta tcgggagctg ttggtagtac aattacggtc
     1861 ttaaaaaaga atgagcgata caaagaaaaa attatatcag attacttggg agtctttttg
     1921 gaaagtaaat cgcagtattt acgagaacat tcaacaggtg caacaattcc tcatttaaac
     1981 aagaatatat tacttgattt acaattagaa ttgctaggta tcgaagaaca agagaacatt
     2041 atctgtattc ttaatacgat taaaaggctt attactaaaa gaaaattgca gttagatgaa
     2101 ctaaacttgc tcg
//
LOCUS       DAVBCG010000180         2089 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVBCG010000180 DAVBCG010000000
VERSION     DAVBCG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.180"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(88..292)
                     /locus_tag="VQ495_001987"
                     /pseudo
     CDS             complement(88..292)
                     /locus_tag="VQ495_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            649..>2089
                     /gene="pcpA"
                     /locus_tag="VQ495_001988"
     CDS             649..>2089
                     /gene="pcpA"
                     /locus_tag="VQ495_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2531901.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGS"
BASE COUNT          754 a          293 c          336 g          706 t
ORIGIN      
        1 gtgtttaagt tgataagctg tttttaacac agctaacatc tcttcaaaag tggtgcgctg
       61 aacaccaaca agacgcttaa atcgtgcatc ggttagttgt ttacttgctt cataatttcg
      121 cagggagtct attgactctt tggtaggtgt caatgttttt ttcatctatc ccgagaatta
      181 ttttcccgcc atttgtattt gcaaatgctg agtaggtttc ccagaaagac tctggaagat
      241 tgtttttagc ttttttgtat tctaaatcaa ccccttcaaa ttttaagtcc atatttttcc
      301 tttacatctg ttttttgtgg ttctggtatt tgttcaagtt gagtgataat atagcgaatt
      361 gaatttcgag agtttttact cagttaattt cttttttaac ccactttaat ttctttttta
      421 acccacttta atttcttttt taacacgggt taaaaaagaa attaaagtgg gttaattttt
      481 cttgacttaa aatttaaaaa tgatatactg tgtttatgga gagatgacaa tttataaaat
      541 gtactactct attctactag ataatgattg aataaaactt tttataacaa cggctagcaa
      601 agttataatt ttatatctat tagaaaataa atatgtaagg aaatttaaat gaaaaaaact
      661 acaatattat cattaactac agctgcggtt attttagcag catatgtccc taatgaacca
      721 atcctagcag atactcctag ttcggaagta atcaaagaga ctaaagttgg aagtattatt
      781 caacaaaata atatcaaata taaggttcta actgtagaag gtaacatagg aactgttcaa
      841 gtgggtaatg gagttactcc tgtagagttt gaagctggtc aagatggaaa accattcacg
      901 attcctacaa aaatcacagt aggtgataaa gtatttaccg ttactgaagt agctagtcaa
      961 gcttttagtt attatccaga tgaaacaggt agaattgtct actatcctag ctctattact
     1021 atcccatcaa gcataaaaaa aatacaaaaa aaaggcttcc atggaagtaa agctaaaact
     1081 attatttttg acaaaggcag tcagctggag aaaattgaag atggagcttt tgatttttct
     1141 gaattagaag agattgaatt gcctgcatct ctagaatata ttggaacaag tgcattttct
     1201 tttagtcaaa aattgaaaaa gctaaccttt tcctcaagtt caaaattaga attaatatca
     1261 catgaggctt ttgctaattt atcaaattta gagaaactaa cattaccaaa atcggttaaa
     1321 acattaggaa gtaatctatt tagactcact actagcttaa aacatgttga tgttgaagaa
     1381 ggaaatgaat cgtttgcctc agttgatggt gttttgtttt caaaagataa aacccaatta
     1441 atttattatc caagtcaaaa aaatgacgaa agttataaaa cgcctaagga gacaaaagaa
     1501 cttgcatcat attcgtttaa taaaaattct tacttgaaaa aactcgaatt gaatgaaggt
     1561 ttagaaaaaa tcggtacttt tgcatttgca gatgcgatta aacttgaaga aattagctta
     1621 ccaaatagtt tagaaactat tgaacgttta gccttttacg gtaatttaga attaaaagaa
     1681 cttatattac cagataatgt taaaaatttt ggtaaacacg ttatgaacgg tttaccaaaa
     1741 ttaaaaagtt taacaattgg taataatatc aactcattgc cgtccttctt cctaagtggc
     1801 gtcttagatt cattaaagga aattcatatt aagaataaaa gtacagagtt ttctgtgaaa
     1861 aaagatacat ttgcaattcc tgaaactgtt aagttctatg taacatcaga acatataaaa
     1921 gatgttctta aatcaaattt atctactagt aatgatatca ttgttgaaaa agtagataat
     1981 ataaaacaag aaactgatgt agctaaacct aaaaagaatt ctaatcaggg agtagttggt
     2041 tgggttaaag acaaaggttt atggtattac ttaaatgaat caggttcaa
//
LOCUS       DAVBCG010000181         2053 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVBCG010000181 DAVBCG010000000
VERSION     DAVBCG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.181"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(271..1878)
                     /locus_tag="VQ495_001989"
     CDS             complement(271..1878)
                     /locus_tag="VQ495_001989"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2531902.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT          576 a          485 c          394 g          598 t
ORIGIN      
        1 ctgatagaga tgttcacatc ttatttcaat tcactataca atccagttcc taaaactttg
       61 ctagaaattg atttggcttt ccgaattaat ttgttcaaat tttatttcat ttcaatatac
      121 tgataaatcc attcactagc acagggcaag agggagtcgc tattgatttc aacggtagca
      181 ggtacataca gaggccccct acctcagata tactgcaata tcatcgtatg caaaaaacct
      241 ctgagatttt tctcaaaggt tctgattttg ctaattgctg ttctcaaccg ctgcagtaac
      301 aaaggcagtg tagagttctt ctggtcggtt tggacggctt gacagttcag ggtgatactg
      361 acaagctaca aagaatttat tttcaggaat ttccacgatt tctaccaaac gattgtctgg
      421 agaaactcct gaaaagacaa aacctgctgc ctcaaactgc tcacggaagg cattattaaa
      481 ctcataacgg tgacggtgac ggcgttgcac cacttcttga ttgtgataag cagcagcagc
      541 cttagagcca cgtttcaact tagacggata aagtcccaaa cgaagggttc cacccatatc
      601 ctcaatatca atctgatcac gcatgatatc aatgataggg tattttgttt ctggtgcaag
      661 ctctgcagaa ttggcacctt caagacctaa aacgtgacga gcaaactcga tacatgtcaa
      721 ctgcattccc aagcagactc ccaacattgg aacatcattt tcacgcgcat agcggatggc
      781 ttggattttc ccttctgtac cacgttgacc aaaaccacct ggtacgatga tcccgtccgc
      841 atcagacaag agttctgcta cattctctgc tgtcacatca ttggcattga cccaattgat
      901 tttaacttct acatcattga catagccaga gtgtttcaag gcttcgacca ctgagatata
      961 ggcatcttgc aactccacat acttaccaac aagggaaatc ttaacttgtt tcttgaggtt
     1021 catgaccttg tccaccatgg ctgaccattc tgtcatatcc gctgctggtg cgtctaattt
     1081 caaatgatca caaacaattt ggtccatccc ttgtgcctgc aagttcagtg gaatttggta
     1141 aaggtgttca acatccaacg attcgataac ggcttctggt gccacatcac agaactgggc
     1201 cagtttattt ttaattcctt gaccagctgg ctcttctgta cgaataacca acatatttgg
     1261 ttggattccc aagccacgca attctttgac agagtgttgg gttggtttgg ttttcatttc
     1321 accagcagcc ttgaggtaag gaagcaaggt tgtatggata tacatgacat tatccgcacc
     1381 cacatctgcc ttcatctgac gaagagcctc taggaatggc aaggactcga tatctcctac
     1441 tgttccacca acctctgtga taatgacatc agagtcggtc gttagagcgg cacgcttgat
     1501 tttttctttc aaagcatctg tgatatgagg aatgacttga acagttgccc caaggtattc
     1561 tccacggcgt tctttacgaa gaacttcact gtaaattttc ccagttgtca cgttggaata
     1621 tttgttgaga ttgatatcga tgaaacgttc atagtgaccc aagtccaaat ctgtctcagc
     1681 tccgtcatct gtcacaaaaa cttccccgtg ctggtaagga ctcatggttc ccggatcaat
     1741 attgatataa gggtcaaact tttgaatggt tactttgaga ccacgatttt tcaagagacg
     1801 gcctagactc gctgccacaa tccctttccc aatagacgat accacaccac cagttacaaa
     1861 aatatatttc gtagacatag attcctcttt ctaaaatgct caaggtcttg ttaactacga
     1921 ggggatatag aaaacaagac cctactaata agaataatct gggacgactg caccagattc
     1981 acaactaata ctcttcgaaa atctcttcaa accgcgtcaa cgtcgccttg ccgtagatat
     2041 gtgttcctga ctt
//
LOCUS       DAVBCG010000182         2029 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVBCG010000182 DAVBCG010000000
VERSION     DAVBCG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.182"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            73..822
                     /gene="truA"
                     /locus_tag="VQ495_001990"
     CDS             73..822
                     /gene="truA"
                     /locus_tag="VQ495_001990"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2531903.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            812..1585
                     /locus_tag="VQ495_001991"
     CDS             812..1585
                     /locus_tag="VQ495_001991"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2531904.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            1563..2024
                     /locus_tag="VQ495_001992"
     CDS             1563..2024
                     /locus_tag="VQ495_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2531905.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT          557 a          396 c          470 g          606 t
ORIGIN      
        1 agatttttat acccatacaa tatttgtttt aggtccttgt ttacagacta atttcttgat
       61 aaaatagagg ttatgacgag atataaagca actatttcct atgatggtta tgcctttgct
      121 ggctttcagc gccagcctca tgcgcgtagc gttcaggaag aaattgaaaa aaccttgacc
      181 agattaaata aagggcaagc cattactgtt cacggtgctg gtaggacaga tagtggggtt
      241 catgccctgg gacaggtcat tcattttgac ctgccttatc agatggatga ggagaaactc
      301 cgttttgcct tggataccca gtctcctgaa gatattgatg tgatttcgat tgagcttgtg
      361 gcagatgatt ttcattgccg ttatgccaag catagcaaga cctatgagtt tactgtggat
      421 agaggacgtc ccaaaaatcc gatgcgccgt cactatgcca cccactttcc ctacccactc
      481 gatgtggaac gaatgcagat tgcaatcaaa aagctagagg gaacccatga ttttaccggt
      541 tttacagcct ctgggactag tgtagaggat aaggttcgca ccatcacaga agctagttta
      601 atagtcgatg agacaggaca atttttgacc tttacctttt caggaaatgg tttcttgtat
      661 aaacagattc gcaatatggt ggggactctt ctcaaaatcg gtaacaaccg catgccagta
      721 gagcagattg atctcatctt ggagaagaag gacaggcaac ttgcaggtcc cactgcagca
      781 ccaaatggtt tgtatttaaa ggagattcgt tatgaagaat aatcgtattt tagcactttc
      841 tggaaatgat atttttagtg gtggtggact gtcagctgat ttggctacct ataccttgaa
      901 cggcttgcat gggtttgtag cagtgacttg tttgacagcc ttgacagaaa aaggatttga
      961 agtctttcca actgatgata ccatttttca acatgaatta gatagcttgc gtgatgtgga
     1021 atttggggga attaagattg gtcttctccc tactgtcagt gtggctgaga aggccttgga
     1081 ctttatcaaa caacgcccag gagtacctgt ggtgttggat cctgtcttgg tctgcaagga
     1141 aacgcatgat gtagctgtca gtgagctctg ccaagagttg attcgctttt tcccttatgt
     1201 cagtgtgatt acgcctaatc tcccagaagc agaattatta tccggtcagg aaattaaaac
     1261 cttggaagac atgaaaactg cagcgcagaa attgcatgat ttaggagcgc cagcagtcat
     1321 tatcaaggga ggcaatcgtc ttagtcagga caaggctgtg gatgtctttt atgatggaca
     1381 gacctttact atcctagaaa atccagttat ccaaggccaa aatgctggtg caggttgtac
     1441 ctttgcctct agcattgcca gtcacttgat taaaggtgat aaacttttgc cagcagtaga
     1501 aagctctaag gctttcgttt atcgtgctat tgcacaagca gatcagtatg gagtaagaca
     1561 atatgaagca aacaaaaaca actaaaatcg cccttgtatc cctattaacc gccctttctg
     1621 tggttctagg ttatttctta aaaatcccaa cacctacagg aattctaact cttttagatg
     1681 ctggtgtctt ctttgcggcc ttttactttg gtagtcgtga aggagcggta gtcggaggac
     1741 tagcaagttt cttgattgac ctcttatcag gctaccctca gtggatgttc tttagcttgg
     1801 tcaaccatgg cttgcaggga tttttcgcag gatttaaagg aaaaagtcag tggttaggcc
     1861 ttattttagc aactattgcc atggtaggag gctacgcctt gggttctact ttgatgaatg
     1921 gctgggcagc agccctccca gaaattctac caaatttcat gcaaaatatg gtagggatga
     1981 ttgtaggatt tattcttagt caaagtatca agaagattaa gtaatactc
//
LOCUS       DAVBCG010000183         1988 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVBCG010000183 DAVBCG010000000
VERSION     DAVBCG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.183"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            450..1925
                     /locus_tag="VQ495_001993"
     CDS             450..1925
                     /locus_tag="VQ495_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2531906.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          592 a          366 c          448 g          582 t
ORIGIN      
        1 ttttgttttt tatcaaaaaa tactttacaa gttcttaaaa acatgatata gtaataaagc
       61 ttagaaaatg agatgatgtt ttctagcaaa tataaacccg agtaaaaaat gcctacggac
      121 aggcagggtt gaatgccgaa gcgtggttga aaagccacat tattgatagg gttaaaagcc
      181 tacttttata agttgatgtt aggacacttg tcctaattca taaattttta gtgtggtgaa
      241 agcacacgtc atcttgtgaa acgatcaata aagtacgtaa tatttgctac tagagagtta
      301 ggaaacatcg ggaacagaca tactcaacag aaaccaaaat aaacacgtca gaagattgca
      361 gagcaggtga aaacctgctc ttttttcatg agtcaacctt tagttcctta gttttcataa
      421 ggtcctaaaa atattgaaag gagtatgttt tgaaagagtt agatcaaaac caagccccaa
      481 tttatgaggc cttggtgaag ttacgcaaga aaaggattgt tccctttgat gttccaggtc
      541 acaagcgtgg acggggaaat ccagaacttg tcgaactctt aggagaaaaa tgtgtaggca
      601 ttgatgtcaa ttcgatgaaa cccttggata atttaggcca tcctatttcg attattcgtg
      661 atgcagagga gctggctgca gatgcttttg gagctagcca tgcctttcta atgattggtg
      721 gaacaacttc atcggtgcag actatgattc tggcaacctg caaggcagga gataagatta
      781 ttctgccacg aaatgtccat aaatctgcta tcaatgcgtt ggttctatgt ggtgccattc
      841 ccatctatat cgagatgagt gtagatccta agattggtat cgctttaggt cttgaaaatg
      901 accgagtagc acaggccata aaggaccatc cagatgctaa ggctatccta atcaacaatc
      961 ctacttacta cggcatctgt tcagacctaa aggggttgac agaaatggct catgaagctg
     1021 gcatgatggt tttagtagat gaagcccatg gagcgcattt gcatttcact ggtaaacttc
     1081 caatttctgc tatggatgca ggagctgata tggcagcagt ttccatgcat aagtctggtg
     1141 ggagtttgac acaaagttcg cttcttttaa tcggggagca gatgaatccc gagtacgttc
     1201 gtcagattat aaacctgacc cagtctacat ctgcctctta cttattgatg gctagtttgg
     1261 atatttcacg tcgcaacttg gcccttcgtg gtaaagagtc gtttgaggaa gtcattgagc
     1321 tatccgagta cgctcgtcat gaaatcaatg ctatcggtgg ctactatgcc tactcaaaag
     1381 agttaataga cggtgtttcg gtttgcgatt ttgacgtaac taagttgtca gtttacactc
     1441 agggtattgg cttaacaggt atcgaggttt atgacctctt gcgagacgaa tacgacattc
     1501 agatcgagtt tggtgatatc ggcaatatct tggcctatat ttccatcggc gaccgcatcc
     1561 aagacatcga gcgcttggtt ggtgctctgg ctgatattaa gagactctat tcaagagatg
     1621 gaaaagattt gatagcagga gaatatattc agcccgagtt agtgctgtct ccgcaagaag
     1681 ccttctattc agaaagaaaa agtttaactt tggatgattc tgttggacag gtctgtggag
     1741 aatttgttat gtgttaccct ccaggtattc ctatcttggc tcctggtgaa cgcattacac
     1801 gagaaattgt cgactatatc caattcgcca aggaacgtgg ttgctccctc caagggacgg
     1861 aagatccaga ggtcaatcat atcaacgtta ttaagagaaa gacaaactat aagaaaagtc
     1921 aatagtttat ctaaactatt tcttatttca atttgatgat ttggcgatga ttttagagca
     1981 cggcaaaa
//
LOCUS       DAVBCG010000184         1927 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVBCG010000184 DAVBCG010000000
VERSION     DAVBCG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.184"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            368..1714
                     /gene="gdhA"
                     /locus_tag="VQ495_001994"
     CDS             368..1714
                     /gene="gdhA"
                     /locus_tag="VQ495_001994"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2531907.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          605 a          352 c          363 g          607 t
ORIGIN      
        1 ttggatttct tctttagttg atttcatatc actattatat aatgattttt gattttagtc
       61 tggtataaat attgctttcc tccaaaatgg tcataatttt actagcaaat ctaacatatc
      121 acggataaat taacaagtga tttctgaatt gctaaacatt ttcttttctt atagcatacc
      181 atactttaag attttgtctt tgagaaagat atttccaaga aaaacgttcg tttttttatc
      241 tattttcaaa aaaaccaaaa actgaattga aagaattttt agaaaatttc tgttttttct
      301 ttacagtgaa aaaaaattct gctataatgt cttatgtaat aaaatatgac aataaaagga
      361 gaacgatatg acatctgcta aagaatatat ccaaagcgtg tttgaaactg tgaaagctcg
      421 taacgggcac gaggctgaat tcctccaagc tgttgaagaa tttttcaaca ctttagaacc
      481 tgtatttgaa aaacaccctg agtatatcga agaaaatatc ttggcacgta ttactgagcc
      541 tgagcgcgtg gtttctttcc gtgttccttg ggttgaccgt gatggaaaaa ttcaagtaaa
      601 ccgtggttac cgtgttcaat tcaactcagc tgttggacca tacaaaggtg gacttcgttt
      661 ccacccaact gtaaaccaag ggatcttgaa attcctcgga tttgaacaaa tctttaaaaa
      721 tgtcttgact ggacttccta tcggtggagg taaaggtgga tcagacttcg atcctaaagg
      781 taaaacagat gctgaagtga tgcgcttctg ccaaagcttc atgactgaat tgcaaaaaca
      841 catcggacca tcacttgacg tacctgctgg tgatatcggt gttggtggac gtgaaattgg
      901 ttacctttac ggtcaataca aacgtcttaa ccaatttgat gctggtgtct tgactggtaa
      961 acctcttgga tttggtggta gcttgattcg tccagaagca actggttacg gtttggttta
     1021 ctatactgaa gaaatgctca aagctaacgg taacagcttt gctggtaaga aagtcgttat
     1081 ttcaggttct ggtaacgttg ctcaatatgc tcttcaaaaa gcaactgaac tcggtgcaac
     1141 tgttatttct gtttctgact caaatggtta tgtcatcgat gaaaatggta tcgacttcga
     1201 tcttttggtt gatgttaaag aaaaacgtcg tgctcgtttg actgagtatg cagctgaaaa
     1261 agcaactgca acctaccacg aaggtactgt atggacttac gctggaaact atgacattgc
     1321 tcttccatgt gccactcaaa acgaaatcaa cggtgaagca gctaaacgtt tggttgctca
     1381 aggcgttatc tgtgtatctg aaggtgccaa catgccgagc gaccttgatg ccatcaaagt
     1441 ttacaaagaa aatggtatct tctacggacc tgcaaaagct gccaacgctg gtggtgtagc
     1501 cgtttcagct cttgaaatga gccaaaacag tcttcgcctc tcatggactc gtgaagaagt
     1561 tgatggccgt ctcaaagaca tcatgacaaa catcttcaac acagctaaaa cgacttcaga
     1621 aacatacggt cttgataaag actaccttgc aggagctaac attgctgcct ttgaaaatgt
     1681 agcaaatgcc atgattgcac aaggtattgt ttaagattta tttactcaat cctcaatcac
     1741 tacgattggg gatttttagc ttgaattaaa aaattggaaa atctaattaa ataaaatgaa
     1801 atgaaataaa aactgaacaa attgattgcg gaattcaaac caatttctaa catacttcta
     1861 gaaattaatt tgacttttct aatcgatttg ttcatatctt atttcaattt actatactta
     1921 cttaacc
//
LOCUS       DAVBCG010000185         1860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVBCG010000185 DAVBCG010000000
VERSION     DAVBCG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.185"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(3..968)
                     /locus_tag="VQ495_001995"
     CDS             complement(3..968)
                     /locus_tag="VQ495_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2531908.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VQ495_001996"
     CDS             complement(1086..1499)
                     /locus_tag="VQ495_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET2531909.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VQ495_001997"
     CDS             complement(1496..1732)
                     /locus_tag="VQ495_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET2531910.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          544 a          362 c          274 g          680 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagcctc
      241 tggacttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtacgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctgata ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaatccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga ggcttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcaaata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
//
LOCUS       DAVBCG010000186         1843 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVBCG010000186 DAVBCG010000000
VERSION     DAVBCG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.186"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(283..675)
                     /gene="rpsI"
                     /locus_tag="VQ495_001998"
     CDS             complement(283..675)
                     /gene="rpsI"
                     /locus_tag="VQ495_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2531911.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(694..1140)
                     /gene="rplM"
                     /locus_tag="VQ495_001999"
     CDS             complement(694..1140)
                     /gene="rplM"
                     /locus_tag="VQ495_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2531912.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1496..1813
                     /locus_tag="VQ495_002000"
     CDS             1496..1813
                     /locus_tag="VQ495_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2531913.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
BASE COUNT          535 a          363 c          347 g          598 t
ORIGIN      
        1 tcctcggttt gtcctattct tatttcacct tactataaaa ataaggtaaa cttttcaaaa
       61 aaacacgaaa aaagcaccca taaggtaaac ttttgagtgc tttgaaacgt tgatataatc
      121 gtattgatta acgttttgag gcaactgacc atgtcaggtt gcagtcacat ttgtaagcga
      181 ctaaagtcgc aacaactgtg tcaattgcac caatttagta agaaagtata aaaaaagaac
      241 accccgaaag gtgctctgta taagtatagt aattctttcg aattaacgtt tactaaattg
      301 tgatgcttta cgagctttct taagacctgg tttcttacgt tcaactttac gtgagtcacg
      361 tgtaagaagt cctgcgcgtt tcaatgaatc gcggaagtct gggtctactt gaagaagggc
      421 acgagcgata ccgtgacgga tagctcctga ttgaccagcg tatccaccac ctataacgtt
      481 aacgaaaacg tcgtatgaac ctacagttga agtaactgcg aatggttggt tgatgacaag
      541 acgaaggtca gcgtgtggga tgtactcttc aacatctttt ttgttaacag tgattttacc
      601 agttcctgga acaaggcgaa cgcgtgcaac agcgttttta cgacgtccag tacctgcata
      661 ttgtgcttgt gacatacttt attgttcctt tccttagata agtcctgaaa tgtcaagaac
      721 ttctggttgt tgtgcagcgt gagtgtgctc agctccaaca aatactttca acttcatacc
      781 ttgagcgcgt ccaagtgtat tgtgtggaag cataccttta actgatttct caatcaaacg
      841 tactgcattt ttagaacgaa gttcacctgc agagatttgt ttcaatccac ctgggtggtt
      901 tgagtgagtg tagtagattt tatcagttgc ttttttacca gtcaatttaa ctttttcagc
      961 attgataaca atcacgaagt cacctgtatc agtgtgtggt gtaaatgttg gtttgttttt
     1021 tccgcgaagt acgctagcaa ctactgcaga aagacgtcca agtggtacat cagttgcgtc
     1081 aactacgtac catttacgtt caacttggcc tggtttagcc ataaatgttg ttttgttcat
     1141 gatttctcct atatatagtt cgatttttgt ttacggtgat gggtgttcct tcccatcgaa
     1201 aagtatttgg aaggttccgg ggcctttcaa atggggtaaa caataccgcc tactatcata
     1261 ccaaatttta cccctaaaag tcaatagata tgaaaaatat ttttcggtaa cttctcaaag
     1321 taactgaaag ttatataatc aagttaattt gggaatttac ctattaactt cggtagattt
     1381 ttaggaattt tttccctatt tctccctgta actaaaatcc ttttcaaaaa atcacctttt
     1441 cttgattttt aatcctattt gctgtatgat aagggaaaag aaaggggaca gagatatggc
     1501 ttttaccaat acccacatgc gatctgctag ttttggcatt gttaccagct tgcctgatga
     1561 catcattgac tctttttggt atatcatcga ccatttctta aaaaatgtct ttgaattgga
     1621 agaagaactc gagtttcaat tgcttaataa ccaaggaaag attaccttcc acttttcaag
     1681 tcaacacctc cctacagcca ttgattttga ctttaaccat cctttcgacc ctcgttatcc
     1741 cccaagagta ctggttttag acatggacgg tagagaaact atcctcctcc cagaagaaaa
     1801 tgacctattt taatactcaa tgaaaatcaa agagcaaact agg
//
LOCUS       DAVBCG010000187         1781 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVBCG010000187 DAVBCG010000000
VERSION     DAVBCG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.187"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..239
                     /locus_tag="VQ495_002001"
     CDS             <1..239
                     /locus_tag="VQ495_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET2531914.1"
                     /translation="EELSNRGYDVHCDAVMVKDVNEDLMKGYDIFTPIAATDLGFEPG
                     IPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            287..1762
                     /locus_tag="VQ495_002002"
     CDS             287..1762
                     /locus_tag="VQ495_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2531915.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          497 a          362 c          360 g          562 t
ORIGIN      
        1 aagaagaact ttcaaaccgt ggttatgatg ttcactgtga tgcagtcatg gtgaaagatg
       61 taaacgaaga ccttatgaaa ggttacgata tctttacacc aatcgctgca acagaccttg
      121 gttttgaacc aggtatccca gttatcgaag ctgggccaat cttattccgt atcccagcaa
      181 tgagcgctcc agtatttgac aatattgaag cagctattaa agaacacgga ttaagctaat
      241 tctaatttgt taacattcat ataacataaa aaagggagga actgttatgg atgcaatctt
      301 tgacctaatc ggaaaggttt tcaatcccat tttagaaatg ggtggacctg tcatcatgtt
      361 aatcattttg acagtattgg ctttactttt tggagtgaaa ttctccaaag cgcttgaagg
      421 tggtatcaaa cttgccatcg ctcttacagg tatcggtgct atcatcggta tgctaaatgg
      481 tgctttctca gcatcacttg caaaattcgt tgaaaacact ggtatccaat tgagtattac
      541 cgacgttggt tgggcaccac tcgcaacaat cacttggggt tctgcttgga cactatactt
      601 cttgctcatc atgttgattg tcaacatagt gatgctagct atgaagaaaa cagatacact
      661 tgacgttgat atctttgata tctggcactt gtctatcaca ggtctcttga ttaaatggta
      721 tgctgataac aatggtgtga gtcaaggggt ttcactcttt attgctacag cagctatcgt
      781 ccttgtcggt gtgttgaaaa ttatcaactc tgacttgatg aaacctacat ttgatgacct
      841 tcttaacgcc ccaagttcat caccaatgac atcaactcac atgaactaca tgatgaaccc
      901 agttatcatg gttttggata agatttttga aaaattcttc ccaggccttg ataaatatga
      961 ctttgatgct gctaaattga acaagaaaat cggtttctgg ggatctaaat tcttcatcgg
     1021 tttcatcctt ggtatcgtta tcggtattat gggaactcca catccaattg caggtgttgc
     1081 agatgcagat aaatggcgtc ttgttatcaa aggatggttg tctcttggtt tgactgccgg
     1141 tgtatctttg gaactcttct cacttatcgg ttcatggttc atcgcagccg tagaaccact
     1201 atcacaaggt attacaaacg ttgctactaa acgtcttcaa ggacgtaaat tcaatatcgg
     1261 tcttgactgg ccattcatcg ctggtcgtgc tgaaatctgg gcttgtgcca acgtacttgc
     1321 accaatcatg ttgattgaag cagtgcttct ttcaaaagtt ggaaatggta tcttgccact
     1381 tgcaggtatc atcgctatgg gtgttactcc agctctcttg gttgtaactc gtggtaaatt
     1441 gctccgtatg attatcttcg gaacactctt gttgccactc ttccttcttt caggtacact
     1501 tattgcacca tttgcaacag aacttgctaa aggtgtaggt gccttcccag aaggtgtgag
     1561 ccaaactcaa ttgattactc actctactct tgaaggacca atcgaaaaac ttcttggttg
     1621 gacaattggt aacactacaa ctggtgatat caaagcaatc cttggtgcag tagtcttcct
     1681 tgtattctat atcggtatct ttgcttggta cagaaaacaa atgatcaaac gtaacgaaga
     1741 gtacgcagca aaagcaaaat aatgcgctcc tataaaatat a
//
LOCUS       DAVBCG010000188         1702 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVBCG010000188 DAVBCG010000000
VERSION     DAVBCG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.188"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(138..>1702)
                     /gene="lepA"
                     /locus_tag="VQ495_002003"
     CDS             complement(138..>1702)
                     /gene="lepA"
                     /locus_tag="VQ495_002003"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2531916.1"
                     /translation="PGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLANVYLALDN
                     DLEIMPIINKIDLPAADPERVRTEIEDVIGLDASEAVLASAKAGIGIEEILEQIVEKV
                     PAPTGDVTAPLKALIFDSVYDAYRGVILQVRVMDGVVKPGDKIQLMSNSKTFDVAEVG
                     IFTPKAVGRDFLATGDVGYIAASIKTVQDTRVGDTVTLATNPAAEPLHGYKQMNPMVF
                     AGLYPIESNKYNDLREALEKLQLNDASLQFEPETSQALGFGFRCGFLGLLHMDVIQER
                     LEREFNIDLIMTAPSVIYKVNLTDGESMDVSNPSEFPDPTKIATIEEPYVKAQIMVPQ
                     EFVGAVMELAQRKRGDFVTMDYIDDNRVNVIYQIPLAEIVFDFFDKLKSSTRGYASFD
                     YELSEYRPSKLVKMDILLNGDKVDALSFIVHKDFAYERGKLIVDKLKKIIPRQQFEVP
                     IQAAIGHKIVARTDIKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRMKSIGSVEVPQ
                     EAFLSVLSMDEE"
BASE COUNT          497 a          401 c          349 g          455 t
ORIGIN      
        1 tacggtaagg cgacgctgac gtggtttgaa gagattttcg aagagtataa aaactaaaaa
       61 agcagaccat ctaagcctgc tttactattg attcttatat aaatttcctg tgaacaagga
      121 aaggcatttc tgataactta ttcttcatcc atactcaaga cgctgaggaa ggcttcttgc
      181 ggaacttcaa ctgatccgat ggatttcatg cgtttcttac cagctttttg tttttcaagg
      241 agtttacgct tacgagaaac gtcaccacca taacatttag caagtacgtt cttacgaagg
      301 gccttgatat cagtacgagc gacaatcttg tgtccaatag ccgcttggat tggaacttca
      361 aattgttggc gagggatgat tttcttgagt ttatcaacga tgagtttccc acgttcgtag
      421 gcaaagtcct tgtgaacgat aaagctgagg gcatccacct tatctccatt gagaagaata
      481 tccattttca ccagcttaga tgggcgatat tctgacaatt cgtagtcaaa gcttgcataa
      541 ccacgtgtcg aagacttaag tttatcaaag aagtcaaaga caatttcagc aagaggaatt
      601 tgatagataa cattgacacg gttatcatca atatagtcca tagtcacaaa gtccccacgc
      661 ttacgctgag ctagctccat tactgctccg acgaactcct gtggtaccat gatttgcgcc
      721 ttgacataag gctcttcaat ggtcgcaatc ttagttgggt ctggaaactc agatgggtta
      781 gacacatcca tagactcacc gtcggtcaaa ttaactttgt aaataacaga cggagctgtc
      841 atgatgaggt caatattgaa ctcacgctct aaacgttcct ggataacatc catatggaga
      901 agtccaagaa atccacaacg gaaaccaaat ccaagtgcct gagatgtttc tggttcaaac
      961 tgaagactag catcattcag ttgcaatttt tcaagcgctt cacgcaggtc attgtacttg
     1021 tttgattcga ttgggtagag acccgcaaag accataggat tcatctgctt ataaccatgt
     1081 aatggttctg ccgcaggatt ggttgccaag gtaacggtat cacccacacg agtatcctga
     1141 accgtcttga tagacgccgc aatgtaacca acatcaccag tcgcaaggaa atcacgacca
     1201 accgcttttg gtgtaaaaat accgacttcg gccacatcaa aggtcttact attgctcatg
     1261 agctgaatct tatcaccagg tttgaccact ccgtccatga cacgcacttg gaggataacc
     1321 ccacggtaag catcgtaaac agagtcgaaa atcaaggcct taagtggcgc cgtcacatca
     1381 cccgttggtg ctggtacttt ttctacaatt tgctcgagga tttcttcaat cccaatacca
     1441 gccttggcag aagccaaaac tgcttcactg gcatccaaac caatcacatc ttcaatctct
     1501 gtacgcacgc gctccggatc tgcagccggc aggtcaattt tattaatgat aggcatgatt
     1561 tccaaatcat tatccaaagc cagataaacg ttggcaagag tttgagcctc aattccttga
     1621 gccgcatcga ccaccaaaat agcaccctca caggcagcta gcgaacgtga aacttcatag
     1681 gtaaagtcaa cgtgccctgg tg
//
LOCUS       DAVBCG010000189         1649 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVBCG010000189 DAVBCG010000000
VERSION     DAVBCG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.189"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            110..409
                     /locus_tag="VQ495_002004"
     CDS             110..409
                     /locus_tag="VQ495_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2531917.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            411..1181
                     /locus_tag="VQ495_002005"
     CDS             411..1181
                     /locus_tag="VQ495_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2531918.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNASFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1201..1365
                     /locus_tag="VQ495_002006"
     CDS             1201..1365
                     /locus_tag="VQ495_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531919.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          606 a          181 c          269 g          593 t
ORIGIN      
        1 agataactat tattacgaaa aaaaattctt gtgtaaaatt ctattgactt tctatattat
       61 ttttgtattt cataacgaat gtaatttaac aagtgaagaa attttaaaga tggattccta
      121 tgctattaat ttctgggaga gtaaaggggt cggtattgac aaaaaaatct tggaatttat
      181 tcaaaaaaat attacagtta ttaaaaataa aaattttttg aataaagatt ttgatattta
      241 tgttaaaaag attcataatt tattctttgt catttctact ttagtttcaa aagagcaata
      301 catggggata gtttcttccc aaatacatat gttcgcaaat aggatgggaa tttctcccac
      361 cattgaagtt accatgctag atttttttct gagggaggat tctgattaat atggaaaaat
      421 atttttttga tatttctatt tttgaaaaaa taaaaaaagt attatcatac caatctaaat
      481 attgggaaat atcgaagcct agggataagt acagtttcaa aagtcaaaat agaatatctg
      541 gctctaatag cattaaattt aaagattttg agttagatta tgaactggag tcacttggta
      601 aaatactttt aaaaaggaga tcgtttttac taaatgaatt tgataagaat gcgagttttg
      661 acaaactgat ttacttgttg aaaactgtat tctctcctac aaattgcaag tttgtatttg
      721 gagaagaggt tcctttattt agctatccta catcaggtgc gttaaattca attactcctt
      781 ttgtttatat tgataatatg gatgaagtaa agaatggtat atatgaagta aagtatgacg
      841 gacttgaatt taaaaaaagt atgaatatta tagacttaga aacatttacc ccaataacaa
      901 gtaaaaacag tgacgtacaa aataattcat ttaaaaaggt aaatatgata attatgttat
      961 gttcgaatta tgaaaatatt ttcaataaat atggattatt agcttatcga cttttgcatt
     1021 tagaggctgg tcatattgca caaaatatcc agcttgtttc tactgcgcta ggaataaaaa
     1081 gtttaccgat gggaggttgg tttgatgata aaatagaaac ttttattaat gaaattagtg
     1141 ataaaaaaaa tatttatctc tatgcaattg cagttggata aatttaagga ggaagtgtag
     1201 atgaacgcag aggataaatt tcgcgaacta gaaaatgaaa ataaacttct aaaacttcat
     1261 aacaaggagc ttgagaatga aattcaaaat ggatggaaat tttggatatg ggtaactgtc
     1321 cctattttag gtttgatata tgctattatt caaaaaattt tttaattatg ctagtttgtc
     1381 aggttgagta gtttttgttc aaaatttaat aaactttttt caatgaagat tatctaaaga
     1441 ttgcttctca gatgagaatg gcttctctat cttatataaa ttatcttcat agagtgtcct
     1501 gttattcatg ttatcacaga atagatagtt tttttggctc tataatattt gtagtgggta
     1561 aatcccctat agatattatg gagcctattt ttgtgtagaa aaaaagtccc atatcatcta
     1621 taatgaaaag caaccaaact atcattaga
//
LOCUS       DAVBCG010000190         1635 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVBCG010000190 DAVBCG010000000
VERSION     DAVBCG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.190"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            119..1381
                     /locus_tag="VQ495_002007"
     CDS             119..1381
                     /locus_tag="VQ495_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2531920.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFSGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT          424 a          370 c          308 g          533 t
ORIGIN      
        1 attttctttg taaattttct atcttttttc aaaatattct tgcatttttc caaaattttt
       61 tgtagaatat agattacata tacagattat tctgaaaatt caaaaaggag tatttatcat
      121 gtcgcaagct atttccttaa atcaatcaac ctgggcaagc aagctaaaag caatgggacc
      181 tggaatccta atggcaactg ccgctgttgg aggttcccac attgtatcct caactcaagc
      241 tggcggttct tacggttggt ctctacttct cttggtcatc ttagccaatg tctttaaata
      301 tccatttttc cgttttggtg ctgaatacac agctgatact ggaaagactt tggttgaagg
      361 ttatgccgaa aaaggaaaac tctatctctg gattttcttt atcctcaatg tcttttcggc
      421 tatggtcaac acggctggtg ttgccattct gtgctcagct atcatcgcca gtgccttccc
      481 aatgattgga cttagcatta ctcagtggtc cctcattctc gttgcaatca tttgggctat
      541 gctactcttt ggaggctaca aacttttaga cggcatggtc aaatggatta tgtctgcctt
      601 aaccattgcg actgttctgg cagttatcat tgcggcggtc aagcatccag aatacagttc
      661 tgattttgtc gagaagacac cttggcaaat ggcagctctg cccttcatcg tctccctcct
      721 aggatggatg ccggctccta ttgaaatttc agccatcaat tcactttggt cagctgaaaa
      781 gagaaagacc gtcaacttta acacagaaga cgctctgttt gactttaaca ctggttatat
      841 tggaacagct atcctagccg tcttctttgt ggcactggga gcactgattc agtatcctac
      901 agggcaggcg gttgaagctg cttcagccaa atacatctct caattcgtgg gcatgtatgc
      961 ctctgttctt ggcgaatggt cccgttactt gattaccttt attgccttcc tctgtatctt
     1021 tggaacagtt ataactgtta tcgatggcta ttctcgcgtt aatcaggaat ctctccgact
     1081 gctaatcagt caaaaagagg acaatcgtaa atctttgaac atctggatga ccatcactgc
     1141 tatcatcggt atcgtcatta tcaagttctt ctctggtcag gtttcaacca tgctccgctt
     1201 tgccatgatt ggctctttcc tgacaacacc tttctttgct cttttgaatt acgccttggt
     1261 aacgcgtgaa aacaaaaatc ttccttcttg gctcaaacac cttgccattg cgggattgat
     1321 tttcctcttt ggcttcgcca tcttctttat ctacgcactc gcaatcggaa aagcagggta
     1381 agggacaagc gcgagatgaa gataaggttt catttcaaga gaaaattcag caaatatttc
     1441 tatgataaaa agcataagaa caaggttttg aagacctgaa cttatgcttt tttacgttct
     1501 taaagactgt ttatactcaa aaaacagttg aacaacttca accacctctt ataagaattt
     1561 tatactattc gagaatctct tcaaaccacg tcagctctat ctgcaacctc aaagctgtgc
     1621 tttgagcaac ctgcg
//
LOCUS       DAVBCG010000191         1580 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVBCG010000191 DAVBCG010000000
VERSION     DAVBCG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.191"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(119..1315)
                     /gene="tuf"
                     /locus_tag="VQ495_002008"
     CDS             complement(119..1315)
                     /gene="tuf"
                     /locus_tag="VQ495_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2531921.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
BASE COUNT          441 a          311 c          328 g          500 t
ORIGIN      
        1 atagtttgct ctttgatttt cattgagtat tagttcaata gctaaaaaaa gaaccttgcc
       61 aagcaagatt cttttagtgt ctgacttaaa taattgttct tctgggaact aaatcgaatt
      121 aagcttcgat ttctgtaacc atacctgaac caacagtacg tccaccctca cggatagaga
      181 atgtagtacc ttgttctacg gcgattgggt gaatcaactc aacatcgatt gtcacgttat
      241 caccaggcat taccatttca gtacctgctg gaagttcgat tgaacctgta acgtcagtag
      301 tacggaagta gaattgtgga cggtagttgt tgaagaatgg agtgtgacgt ccaccttctt
      361 ctttagtaag gatgtagact tcacctttga atttagtgtg tgggttgatt gaacctggtt
      421 tagcgataac ttgtccacgt tcgatttcat cacgttgaac accacgaaga aggacaccta
      481 cgttatctcc agcaagacct tcgtcaagtt gtttacggaa catttcaaca ccagtaacaa
      541 ctgctttttg agtttcttct ttgataccaa cgatttcgat ttcgtcgttg actttaacga
      601 taccacggtc gatacgtcct gaagcaactg taccacgtcc agtgattgag aatacgtcct
      661 cgactggaag aagcaatggt ttgtcagtgt cacgttctgg ttctgggata tactcatcaa
      721 ctgtgttcat caattccata acgatgtctt cgtatttaga gtcaccttca agagctttaa
      781 gtgctgaacc ttggataact ggaagatcgt cacctgggaa gtcgtattct gacaataggt
      841 cacggatttc catttcaacc aattcaagca attcttcgtc gtcaaccaag tcaactttgt
      901 tcatgaagac gataaggtgt ttaacaccaa cctgacgtga aagaaggatg tgctcacgag
      961 tttgtggcat tggtccgtca gttgaagcta ctacaaggat agctccgtcc atttgagcag
     1021 caccagtgat catgttttta acgtagtccg cgtgtcctgg agcgtcgatg tgagcgtagt
     1081 gacgtttttc agtttcgtac tcaacgtgcg cagtgttgat agtgataccg cgttcgcgtt
     1141 cttctggagc agcatcgata gacgcatagt ctttaggttg gttaactgat gaaggcaagc
     1201 gacgtgccaa aacagttgtg atagctgcag ttagggtagt tttaccgtgg tcaacgtgtc
     1261 cgatagtacc aatgttaacg tgtggtttac tacgatcgta tttttctttt gccatttgag
     1321 taaaagcctc caataaaata tattttatag atagacagta ggcaatacag tctaactttc
     1381 cttactattt tatcaaattt cagctaaatt gcaagtgttt tacaacgttt ttgtgaatta
     1441 ttcttggtaa aaatttaact aattaataga aagtactaaa tagacttatc cttcaaggag
     1501 ttttcagcat tatccatcca ctcacttgct gaaaatgtaa acagtctctg gtttatatag
     1561 taaaatgaaa caagaataga
//
LOCUS       DAVBCG010000192         1562 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVBCG010000192 DAVBCG010000000
VERSION     DAVBCG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.192"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(232..996)
                     /locus_tag="VQ495_002009"
     CDS             complement(232..996)
                     /locus_tag="VQ495_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2531922.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            1066..1227
                     /locus_tag="VQ495_002010"
     CDS             1066..1227
                     /locus_tag="VQ495_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2531923.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            1217..1513
                     /locus_tag="VQ495_002011"
     CDS             1217..1513
                     /locus_tag="VQ495_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2531924.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
BASE COUNT          488 a          391 c          296 g          387 t
ORIGIN      
        1 tggtttgaaa agattttcga agagtatcat ttcatctcac tctaaatctt accaacataa
       61 aacgcatagt atcaaggttt tcatcacctg atatgatacg tttttctgat tttaaagatt
      121 tttcccaacc tcaacatgct taggacaaga ggaaggtgac ctcgtttaaa catatttttg
      181 aaaagtatga gctagacatt ttatgataaa acaaatcctt tgccagtaca actaaaggtg
      241 gtgaacactt gctaaactca gttctagcgc cttattaaaa gcttccgagc cccagtcacg
      301 actgtcgtac tggtccaagt ccgctagaga atctgctgtg aacaacaatt caccccagag
      361 aaccccacgc aattgagcta ctgccgcaag agcagaacac tccatctcca caacagcaca
      421 gccttcttcc ttacgataag ccaccttttc agccgtttct cggtaaaaac cgtctgtcgt
      481 ccaggtcatg acttcttcat aaggaatccc tctgtcttcc aaaacttcct caatagcagc
      541 aatagcctct ggctgcattt ccatataacg agaaggtgcc acatagtggt aactggctcc
      601 ttcatctcgc agagcgcgaa cagggactag aaaggcattt tcctctatat cagctagaac
      661 accacaggtc ccagtagaga taatctgctc cacaccatag ccaatcaacc aatccataaa
      721 ctgggctgct ggagcggagc caacaggagc ctgagccaga cagacctcct cacccttgta
      781 gttgatgaca tagactggat aggtcttggt agcagaaacg aattcgccga cacaatccgc
      841 ccctacttcc cttgcatagc ggtcaatctc ctcatctaaa aatgcataaa cacacttctt
      901 tggcaactgc aagtccagcc cctcgtgatt gggcatgata accgcctgag gattgtcatc
      961 aaactctaaa ataggaatcg catgtttctg aatcatagcc cacctcctct aattttgtac
     1021 tattgtatca aaaactgaca aagtgtcaag gaggaagctg atgaactgga caaaaaacaa
     1081 gcctacatcg tcagctgcca cagtggtttg cgcagctata tcgcagagcc tatcctcaag
     1141 caagcaggat ttaccgccca aaaccttgac ggcgcttatt cactatacaa aatggttaac
     1201 ccagaaggag tagaatatgg aaactagtat cagcatggct gacttttatg gaaaatacca
     1261 aaatgaaaat ctagaactta tcgatgtccg tgaagcacat gaattccaag caggacatgc
     1321 accaggtgcc aaaaatcttc cattaagtac cttagagcaa ggctacaaag aactcaaacc
     1381 tgaccatgaa tactatgtta tttgtcaagg aggagtccgc tctgcatcta cctgccaatt
     1441 tctcatctcc caaggcctca ccgttaccaa cgtagaaggt ggtatgaatg cttggcctgg
     1501 tcaagtagaa taatcaagag gaactgggca aaaactcaat ttcatgagaa gatgaagtaa
     1561 ta
//
LOCUS       DAVBCG010000193         1549 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVBCG010000193 DAVBCG010000000
VERSION     DAVBCG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.193"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(6..605)
                     /locus_tag="VQ495_002012"
     CDS             complement(6..605)
                     /locus_tag="VQ495_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2531925.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            1075..1374
                     /locus_tag="VQ495_002013"
     CDS             1075..1374
                     /locus_tag="VQ495_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2531926.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          582 a          296 c          244 g          427 t
ORIGIN      
        1 tgccactagg caaaaagcat actgagataa aaaatgctca tcataatagg aacaccaaga
       61 atggtctttt catgatagaa aatcgtcaaa taggctgaaa agacatcgcc aaggacaaaa
      121 ctactaagca ggctaacaaa tatgaaccct tcacgcaaaa aaggagtgtg cttagttcgg
      181 aaataatctc caaaagctag cattgttcgt ttgatattcc ctgtcataaa ggcattattg
      241 taggcaatac ccgaaacctc accaaaagca gttgtcacca gtcccataca gaaggccaag
      301 ggctgcacta gatagatatt atctacagtt tgtggcacaa aaccaataat tattgatagg
      361 attgctaaag gaatcaagga cagaataggt tttttcacaa ttctcaattt ttccttataa
      421 atcgttaata aaaagactcc cattataaac gttattaagg ttaaaagctt atccctaaca
      481 tccgaaacat tattttgtat tagctctacc gaaagaaaga caacatttcc agcttgtcca
      541 gctacaaggg tattccctcg aacaataaaa gtgtaagcat ccacatatcc agcacaaaat
      601 gtcaaaaaag tgctaacctt ttagactgac gtgatatttt tcttataggt aataacctca
      661 ttttccctcc cattgtattt tctcttagaa atattgtacc atttctttct aaaaaatcgt
      721 agactaccat ttagatttta ctattagcat aaaaataata atagacaact atttatccaa
      781 aaatagatag aggtaacatg tttgcaaaca aagcatacga acctttagta aaatcatttc
      841 catgaaacta gaatagagcc cctcttagca aaaatcatta ttttaattta tttctaatca
      901 ctccttgaca taaataactc tcaccaataa aagactatgt cttaaaaaaa tggtataata
      961 aaatcaatac ctgggcttga tggctatgct actaataaca attaggagag aaaatcaggc
     1021 acttgttaac aacaaggatt atccccttga gatgaaagga actttagaaa tcttatgatg
     1081 aacatgcaaa acatgatgcg tcaagcacaa aaacttcaaa aacaaatgga acaaagccaa
     1141 gctgaacttg ctgctatgca atttgttggc aaatctgctc aagatcttgt ccaagcgacc
     1201 ttaactggcg ataagaaagt tgtcagcatt gatttcaatc cagctgtcgt tgacccagag
     1261 gaccttgaga ctctttctga tatgaccgtt caagccatca actctgctct tgaacaaatc
     1321 gatgaaacta ctaagaaaaa actgggtgct ttcgctggga aattgccatt ctaaaaacaa
     1381 ggagctagaa caatacttgt cgataacaaa ggctaagaaa ggtgcaaaaa ttataaaaaa
     1441 cgcatagtat caagcaacct aaaactttgg gctctataat atttgtagtg ggtaaatcca
     1501 ctatagatat tatggagcct attttattgt agaaaaaaat cccataaga
//
LOCUS       DAVBCG010000194         1453 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVBCG010000194 DAVBCG010000000
VERSION     DAVBCG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.194"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            122..1273
                     /locus_tag="VQ495_002014"
     CDS             122..1273
                     /locus_tag="VQ495_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2531927.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
BASE COUNT          362 a          267 c          320 g          504 t
ORIGIN      
        1 acaagggatt cattttcgaa tttaagaagg agtctacaaa ccaatgaggc ttcttctttt
       61 cttgcttatt tgataactat caaaaaagta gaagattaag agaaatatag aaagagattt
      121 catgaaacaa tttttagaac gggccagcat tttggctctc tccctcgttt tgattacctc
      181 cttttccatt tcgagtgccc taccagccat gtttgactat tatcagggtt attctaagga
      241 acaaattgag ctcttggtga gcttgccttc ctttggaatc atgatgatgt tactgctaaa
      301 tggtttctta gaaaaaatat ttcctgagcg cttacagatt agtttgggct tgctgatttt
      361 atcattgagc ggtacagctc ccttctggta ccaagcctat ccctttgtct ttggaacacg
      421 gcttctcttt ggtttgggtc ttgggatgat caatgccaag gccatttcta ttatcagtga
      481 acgctaccaa ggaaaaaggc gaattcagat gttagggcta cgcgcttctg cagaggtcgt
      541 tggagcttct ctcattacct tggccgtcgg tcagttgttg gcctttggtt ggacagctat
      601 ctttctagcc tatagtgctg gatttttggt gctgcccctt tatctgctct ttgtccctta
      661 tggaaaatca aagaaagaag tcaagaaaag agcgaaggaa gcaagtcgtt taactcgaga
      721 aatgaaaggc ttgattttta ccttagctat cgaagcggca gttgtagttt gtaccaatac
      781 agctattacc atccgtattc caagtttgat ggtggaaaga ggattggggg atgcccagtt
      841 atctagtttt gttcttagta tcatgcagtt gatcgggatt gtggctgggg tgagtttttc
      901 tttcttgatt tctatcttta aagagaaact gctcctctgg tctggtatta cctttggctt
      961 ggggcaaatc gtgattgcct tgtcttcatc cttgtgggtg gtagtagcag gaagtgttct
     1021 ggctggattt gcctatagtg tagtcttgac gacggtcttt caacttgtct ctgaacgaat
     1081 tccagctaaa ctcctcaatc aagcaacttc atttgctgta ttaggctgta gtttcggagc
     1141 ctttacgacc ccatttgttc taggtgcaat tggcttacta actcacaatg ggatgttggt
     1201 ctttagtatc ttaggaggtt ggttgattgt aatctctatc tttgtcatgt acctacttca
     1261 gaagagagct taggattgat tcctaagttt ttcttttgat actcaatgaa aatcaaagag
     1321 catagttgat tgagtttgga atagtacgct gtagctgcta aaatatttct agaaattaat
     1381 ttgacttttc taatagagtt attcatatct tatttcaatc tactatagaa agctagccgc
     1441 aggttgctca aaa
//
LOCUS       DAVBCG010000195         1452 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVBCG010000195 DAVBCG010000000
VERSION     DAVBCG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.195"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            298..1170
                     /locus_tag="VQ495_002015"
     CDS             298..1170
                     /locus_tag="VQ495_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2531928.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            1183..1347
                     /locus_tag="VQ495_002016"
     CDS             1183..1347
                     /locus_tag="VQ495_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531929.1"
                     /translation="MTVILGFLFLFLMTWWVISIVNSQKPRKNETFIGYIQRYDIDGN
                     GYQSLPCPQN"
     gene            complement(1316..1452)
                     /locus_tag="VQ495_002017"
                     /pseudo
     CDS             complement(1316..1452)
                     /locus_tag="VQ495_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          484 a          259 c          284 g          425 t
ORIGIN      
        1 tactatgttt ttcacctcta acaatcgcaa tctcttcttt atccatgaat gaaatcgctt
       61 tctatttttg taagtaaagc atcacacgaa atccacgaaa atgaaaacct ttgttgtgtt
      121 ttcgtaaaaa atttgttgac agagcacgaa acgcctacta taattaaatc aagcttaagg
      181 aaataacaaa aaaacaaatc ggagggaaag acgatgaaca cattaaatga gaaagtaatc
      241 aatatctgta aagcagtagt taaagaaact ttaatccaag acatttagga gaaagaaatg
      301 aagaaatcaa gaaaattagc cacattagga atctgttcag ccttgttttt aggcttggct
      361 gcttgtcaac aacaacatgc tacttctgag gggacgaatc aaaggcaaag cagttcagcg
      421 aaagttccat ggaaagcttc atacaccaac ctaaacaacc aggtaagtac agaagaggtc
      481 aaatctctct tatcagctca cttggatcca aatagtgttg atgcattttt taatctcgtt
      541 aatgactata ataccattgt cggctcaact ggcttatcag gagatttcac ttcctttact
      601 cacaccgaat acgatgttga gaaaatcagt catctctgga atcaaaagaa gggcgatttc
      661 gttgggacca actgccgtat caatagttat tgtcttttga aaaattcagt caccattcca
      721 aagcttgaaa agaatgacca attgcttttc ctagataacg atgcgattga taaaggaaag
      781 gtctttgatt cacaagataa ggaagagttt gatattctat tttcgagagt tccaactgag
      841 gcaactacag atgtcaaggt tcacgctgaa aagatggaaa cattcttctc acaatttcaa
      901 ttcaatgaaa aagctcgaat gctgtctgta gtcttgcacg acaatctgga tggcgagtat
      961 ctgtttgtag gccatgttgg ggtcttagta cctgctgatg acggtttctt atttgtagag
     1021 aaattgactt tcgaagagcc ctaccaagcg attaaatttg ctagtaagga agattgctac
     1081 aagtatttgg gcaccaagta tgcggattat acaggcgagg gactggctaa gccttttatc
     1141 atggataatg ataagtgggt taaactttaa tagtgttatg atatgacagt aatcttagga
     1201 tttttatttc tatttttgat gacttggtgg gttatttcaa tcgttaatag ccagaaacca
     1261 cgcaaaaatg aaacatttat tggctatatc caaagatacg atattgatgg gaatggctat
     1321 caatcattac cgtgtcctca gaactgagag gagttcttga aaccgtaaaa ccgctttgaa
     1381 caagagttac ttcaacccat tggctccgac ggagtaagtt gctttcgtga ataccaaaat
     1441 cagccgcaat tt
//
LOCUS       DAVBCG010000196         1445 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVBCG010000196 DAVBCG010000000
VERSION     DAVBCG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.196"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(105..1295)
                     /locus_tag="VQ495_002018"
     CDS             complement(105..1295)
                     /locus_tag="VQ495_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2531930.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT          457 a          232 c          314 g          442 t
ORIGIN      
        1 aatattctta tcaattgatt tgaatgccaa aattccatcg ttccggtttt atttcatttg
       61 gctataaaaa actctcctgg gaaatcccag gagagtatga ttacttattt gaacttgaat
      121 ccttcgtaaa taagctctgt ttttggattt tgtttcttaa tctgtttggc aagtgccttc
      181 atcatagaaa taggaccaca catatagacg gttgcatgtt cgggcacttc tttttgttca
      241 aaattaagat agccgtcttt cgtactgtcg attagatgga gttcaaaatt aggatttttc
      301 tgagcatagt tacggagtaa atctaggtag actgcatttt catctccacg gaagctatag
      361 tagaagtgaa cctgtttatc taaaatagga tgttcacgga tgtaagagat gaagggggtg
      421 atcccaatac ctccagcaat ccaaacctga ttttctcgtc cttcttctat gatcatgtgt
      481 ccgtaagctc tgtctagggt tactttgctg ccggcttgaa gattatcata gatattattg
      541 gtatggtcgc ctgaattttt aacagtaaag taaagagttt gaccatgacc tcctgagata
      601 gaaaagggat gcggagcact ttcaaagcct tcttggaaaa tctttagaaa ggcaaattgt
      661 cctgattgat agttgaaagg tctgctaaga tggatttgaa tttctctagt atcgtgattt
      721 aagcgtttga gatgggtaat ttttcctaga taggggaagg aaatcttttg atatagaaaa
      781 atgatataaa aaccagctag taagcctaaa agggcatagc taccaacaag aaaacttaga
      841 agattaaatg taaggagacg attgcccatt atcatgtaga tgtgaaagag tcctaaaata
      901 taggctaggt aaaccaggcg gtgaatccat cgccaagctt cgtattggat gtatttgcct
      961 aaataggcga caaggatgat gctggcaaag atatagatgg caagattacc aaactgagca
     1021 gctaagcgag agccccacaa accgcccata ctaaagttat gaaagattag taggatgatg
     1081 gagagaaagg ctgtgaattt gtggacggtg tagaccttct ccaaaccgtg aaaccagctt
     1141 tctagtagtg ggagacgagt ggctaggata aaagtcagag ataggcttgt taaagctagt
     1201 cctggaatca tgaattgggg agaagtgttc atccaagtca aaagagtcaa gataaaacta
     1261 gctatgataa agagtagtcc tttgactgat ttcatagaaa attccatttc atttagattt
     1321 cgatttgttg taaatgaatt tgttacattt tatcatagaa aatgtatggt gtcaaattga
     1381 ggtctataaa tatctactct catcaaaaaa ctctccaatt gaactggaga gtggctgttt
     1441 atact
//
LOCUS       DAVBCG010000197         1380 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVBCG010000197 DAVBCG010000000
VERSION     DAVBCG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.197"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            256..1266
                     /gene="ccpA"
                     /locus_tag="VQ495_002019"
     CDS             256..1266
                     /gene="ccpA"
                     /locus_tag="VQ495_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2531931.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKVGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
BASE COUNT          434 a          226 c          316 g          404 t
ORIGIN      
        1 ctttagaagg gcttagtttc ctccttttta tttagtctaa ggtggtagca aggagctaaa
       61 cctaggtttt ctatgaaatt ttttcaatca atcttcaaaa aacacttgaa agtgtttaca
      121 attagtgata gaatggaata agtagaaata tgaaaacgaa attttcacat cttcactttt
      181 gtaagtagat atagagcttc aatcagtacc tctacttgta aaagaatgtg tgagatagaa
      241 aggacaggaa aaggaatgaa tgcagatgat acagtaacca tttatgatgt cgctcgtgaa
      301 gcaggtgttt ccatggcgac ggtcagccgt gtggtcaatg gcaataaaaa tgtaaaagag
      361 aatacccgta aaaaagtgct agaggtaatt gatcgtttgg attatcgtcc aaatgcagtt
      421 gcgcgtggtc ttgcaagtaa aaagacaacc actgtcggtg tcgtgattcc aaatattacc
      481 aatggttatt tttcgagttt ggctaagggg attgatgata ttgcagaaat gtacaagtac
      541 aatattgtcc tagctaatag cgatgaagat aacgagaaag aagtttctgt tgtcaatacc
      601 ctcttttcaa agcaggtaga tggcattatc tatatgggtt atcacttgac agataaaatt
      661 cgctcagaat tttcgcgttc acgtactccg attgttctcg caggaactgt cgatgttgag
      721 caccagttgc caagtgtcaa tattgactat aagcaagcaa caattgatgc agtgagctac
      781 cttgctaaag aaaatgagcg tattgctttc gttagcggtc cgctagtgga tgacatcaat
      841 ggtaaggttc gtttagttgg ctacaaggaa accttgaaaa aagtaggaat cacttatagt
      901 gagggtttgg tatttgaatc taaatatagc tatgatgatg gttacgcctt agcagagcgt
      961 ttgatttcat caaatgcaac tgcagcagtt gtgacaggtg atgagttggc agcaggagtc
     1021 ttgaacggtt tggctgataa gggtgtttct gtgccagaag attttgaaat tattactagt
     1081 gatgattcac aaatctcacg ctttacccgt ccaaacttga caacgattgc ccaacctctt
     1141 tatgaccttg gtgccattag tatgcgtatg ttgaccaaga ttatgcataa ggaagagttg
     1201 gaagaacgtg aagttctctt acctcatggt ttgacagaac gtagctcaac acgaaaacgt
     1261 aaatagaaaa aatcagggaa tcgagaagat ttcctgattt tgtgctctta aacaagagat
     1321 gttgacttaa tgaataccat agagcggact aggcggctgg ttaaaggctg cgcaacacac
//
LOCUS       DAVBCG010000198         1361 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVBCG010000198 DAVBCG010000000
VERSION     DAVBCG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.198"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(86..226)
                     /locus_tag="VQ495_002020"
     CDS             complement(86..226)
                     /locus_tag="VQ495_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531932.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(223..405)
                     /locus_tag="VQ495_002021"
     CDS             complement(223..405)
                     /locus_tag="VQ495_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531933.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(407..1190)
                     /locus_tag="VQ495_002022"
                     /pseudo
     CDS             complement(407..1190)
                     /locus_tag="VQ495_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          451 a          294 c          198 g          418 t
ORIGIN      
        1 ttactgctta tattatacca tatggagaga gattaaaaaa ctagcagtgg agttcctgct
       61 agttttctgc gacttggatt accatctaga taagtttaaa gaaccaatcc aatatcttgt
      121 aaaacagcaa ttgaactgca aaagaaatga tcatccaaaa gaatttcaca atcccaataa
      181 tcggtttgat aaaaagaata gcaagaattg tccagaaaaa tttcattctc tctcctctgg
      241 cttaatgagc caccgagccg tatccattag cttatctgca ataaagagtt ttcccagacc
      301 aacaacgaca tggtcatctt tctttatcgt tttcatcact tttacacctt gcacggtcaa
      361 aaagtaatta ccataaattt tagcaaaagc tttgatagga ttcatattat tctcctttga
      421 tgatttcagc ctcttttcgg attgtttcaa catcttcttg atattgaact tcactatagt
      481 tgactagctt ggataattct ttctgcaagc tttcccccat tggtttcata gttgcaaagg
      541 ttaaaccacc tgaaatgata ccacccaaga taggaataaa tttccccatt cctttggcca
      601 accctccctt ggtaagattc acaccaaata tttttaagac ttttttcaaa atagggtacc
      661 aaagcgtctt tgttaaagct ttattaggca ctgttttcat tacctgtttg gcaattgtta
      721 taccaccagc acgtagcaaa caaagcagcg gttccattca cccctaacat tacgcctaga
      781 taaagcaaga gggtattttg agcatcttca ctcaactcct cacgtgaagc ccaaagatcc
      841 tcataaccat aaatataacc taattcttgg gccaatttca gagagaaacc ataaaattga
      901 gccacataag ctggaatggt aattgccata gcaagccctc caggtaatcc tgccaaaaca
      961 gaagtcccac tcgctaataa aacattatcc ctaatacagg cattagccac tcgatctaag
     1021 atttcttgag ataagagaga cgttggacct tgttccaata aggtaggaat gtcctgtgga
     1081 cccaattctt tggaaaactt atccactaaa aatttcgaac gatcaacctt aacaactggt
     1141 agttttacca cttgttgtaa tacttgcata gctaagtctt gttcagccat atacaaactc
     1201 cttatttctt ttttgcacct tatcatatta tatttcctac aaaaaaagca ttttctaatt
     1261 agataacatt aaaaaataca gaaccccttt tttatagtaa aataaaacaa gaacaggaca
     1321 aatcgatcag gacagtcaaa tcgatttcta acaatgtttt a
//
LOCUS       DAVBCG010000199         1266 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVBCG010000199 DAVBCG010000000
VERSION     DAVBCG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.199"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>1266
                     /locus_tag="VQ495_002023"
     rRNA            <1..>1266
                     /locus_tag="VQ495_002023"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          334 a          282 c          373 g          277 t
ORIGIN      
        1 agcgaagttt agtagcgaag ttagtgacgt cacactgcca agaaaagctt ctagcgttta
       61 aacatactct acccgtaccg caaaccgaca caggtagtcg aggcgagtag cctcaggtga
      121 gcgagagaac tctcgttaag gaactcggca aaatgacccc gtaacttcgg gagaaggggt
      181 gctgacttta agtcagccgc agtgaatagg cccaagcaac tgtttatcaa aaacacagct
      241 ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct gcccggtgct ggaaggttaa
      301 gaggagtgct tagcgtaagc gaaggtatga attgaagccc cagtaaacgg cggccgtaac
      361 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg cacgaaaggc
      421 gtaatgattt gggcactgtc tcaacgagag actcggtgaa attttagtac ctgtgaagat
      481 gcaggttacc cgcgacagga cggaaagacc ccatggagct ttactgcagt ttgatattga
      541 gtgtctgtac cacatgtaca ggataggtag gagtctaaga gatcgggacg ccagtttcga
      601 aggagacgct gttgggatac tacccttgtg ttatggccac tctaacccag ataggtgatc
      661 cctatcggag acagtgtctg acgggcagtt tgactggggc ggtcgcctcc taaaaggtaa
      721 cggaggcgcc caaaggttcc ctcagaatgg ttggaaatca ttcgcagagt gtaaaggtat
      781 aagggagctt gactgcgaga gctacaactc gagcagggac gaaagtcggg cttagtgatc
      841 cggtggttcc gtatggaagg gccatcgctc aacggataaa agctaccctg gggataacag
      901 gcttatctcc cccaagagtt cacatcgacg gggaggtttg gcacctcgat gtcggctcgt
      961 cgcatcctgg ggctgtagtc ggtcccaagg gttgggctgt tcgcccatta aagcggcacg
     1021 cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgcgg gcgtaggaaa
     1081 tttgagagga tctgctccta gtacgagagg accagagtgg acttaccgct ggtgtaccag
     1141 ttgtcttgcc aaaggcatcg ctgggtagct atgtagggaa gggataaacg ctgaaagcat
     1201 ctaagtgtga aacccacctc aagatgagat ttcccatgat tatatatcag taagagccct
     1261 gagaga
//
LOCUS       DAVBCG010000200         1254 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVBCG010000200 DAVBCG010000000
VERSION     DAVBCG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.200"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            159..1190
                     /locus_tag="VQ495_002024"
     CDS             159..1190
                     /locus_tag="VQ495_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2531934.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRALFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
BASE COUNT          373 a          256 c          258 g          367 t
ORIGIN      
        1 gcaggttgct caaagcacag ctttgaggtt gcagataaaa ctgacgtggt ttgaagagat
       61 tttcgaagag tattaaaagt attcttctga aatcccacat agctttctct tatattttgt
      121 gataaaatag gctcaatcta tttctaggag gatgagatat ggtttctact attggtattg
      181 ttagtttatc tagtggcatt atcggagagg attttgtcaa acacgaagtg gacttgggta
      241 tccaacgtct caaggatctg ggactcaatc ccatcttttt gccccattcg ttaaaaggat
      301 tagactttat caaggaccat cctgaagctc gtgcagagga tttgattcat gccttttctg
      361 atgatagcat cgacatgatc ctatgtgcca tcggtggaga cgatacctat cgcttgctac
      421 cttatctttt tgaaaatgac caactccaaa aggttatcaa gcaaaaaatt tttcttggct
      481 tctcggatac aaccatgaac cacctcatgt tgcataaatt aggaatcaag actttttatg
      541 gtcaatcctt tttagcagac atttgtgaat tagacaaaga aatgctagcc tatagccttc
      601 actactttaa agaattgatt gagacgggaa gaatttcaga aatccgccct agtgacgttt
      661 ggtatgagga acgaactgat tttagtccca cggccctggg aacacctcgt gtcagtcata
      721 caaatacagg ttttgacttg ttgcaaggaa gggcactatt cgaggggaaa atcctcggtg
      781 gttgcctcga atccctctac gatatctttg acaactctcg atacgcagat agcacggagc
      841 tctgccaaaa atacaaactt ttccctgact tgtcagactg ggaagggaaa atcctcttgc
      901 tcgaaacaag cgaagaaaag cctaagccag aagacttcaa aaagatgttg ctaactttaa
      961 aggacactgg catattcgcg gtcataaatg gactcttggt cggaaaacct atggatgaaa
     1021 ctttccatga cgactataaa gaggcactat tggatatcat tgacagcaat atcccgattg
     1081 tctataatct gaatgccggc cacgcaactc caagagcaat tgtacccttt ggggtccatg
     1141 ctcatgtaga tgcacaggaa caagtcattc gctttgacta taacaaataa agctgggaaa
     1201 agtttctcag cttttttctc ggtatcatta ctggcatttt catcacatat taca
//
LOCUS       DAVBCG010000201         1205 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVBCG010000201 DAVBCG010000000
VERSION     DAVBCG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.201"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            503..728
                     /locus_tag="VQ495_002025"
                     /pseudo
     CDS             503..728
                     /locus_tag="VQ495_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          454 a          237 c          208 g          306 t
ORIGIN      
        1 ctagaaatgt tttagcagct acagcgtact attccaaact caatcaacta taaaacaata
       61 tagactagta atggttggac tcaataagaa ttgggtacta agattggtat aagtaaaaac
      121 accattggaa attatgaaaa aagggttagg tctactaaga aaaatactat atttgactta
      181 gctaaagtat tcagtagctt gattgatacc ctctttcctc cagttcaaaa ggattctcct
      241 agcgatatcc aatctatcta cgaccaacgg gcaccaccca gacaaggtaa agttctgacc
      301 tatgccgaga ggcaactgta tgagcagaaa aacgaaatat cgtaaaaatc atcagactgg
      361 acgactacag acagactact tactctcgtg ttaggtagtt tatctctgca ggtagccgct
      421 cgatgcagga cgacgattta gttatggagt ttccattatc ccagacgact acgacccaat
      481 cgcttatgtc gtcagcaagt ccatggagcc aaagataaga aacagcgact acctatttat
      541 caagaatacc ccacagattg actataatac catcggcatc ttccaagtag acggcgctaa
      601 ctatgtcaag aaactccgcc aaggctacct taaaaaaact caatcccaaa taccccgaca
      661 tccacctaga cgaaagcaac catattcgta ccatcgggaa agttgtcagg ctatataggg
      721 agaattaaaa aagggggaga atggcgatat aaaacaaaca gattttaaaa gattcgaaaa
      781 actttgaaaa agttcattct acatttcaaa aaaagaataa gtgacctaaa gtagattaga
      841 ttttgttaaa caatagtgat atgtaaattt agagaaaggc atttaacatc actttgcaaa
      901 aggcaaatac taaactagag aaaccgataa accagcccct tagttcccat attttcaggc
      961 acacactcct tagtacacta gctgagaaga atgctataat ggttagagtg gaacacaaaa
     1021 acgctaaaac agtaaataac atctacactc acgtaagtaa aagaatggag caagctactt
     1081 tggaggtttt aaataccata tcattaaatc gaaaatatat cagatcgaat ctagacaagt
     1141 acattacgat tgctaaaaca tttgtagaaa ttcatttgaa tttcctaatc aatttgtgca
     1201 tatct
//
LOCUS       DAVBCG010000202         1203 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVBCG010000202 DAVBCG010000000
VERSION     DAVBCG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.202"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            236..676
                     /locus_tag="VQ495_002026"
     CDS             236..676
                     /locus_tag="VQ495_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2531935.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            673..1131
                     /locus_tag="VQ495_002027"
     CDS             673..1131
                     /locus_tag="VQ495_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2531936.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          375 a          269 c          229 g          330 t
ORIGIN      
        1 ccttgccaga gggagtaaaa tagtggtata ctcaataaaa atcaaagagc aaactagaaa
       61 gctaggcgca gacagctcaa aacactgttt tgaggttgta gataaaactg acaaagtcaa
      121 caactatcct acggcaagac gacgctgacg tggtttgaag agatttttga agagtattaa
      181 taaaggtgta caagaagtga gattaatttt gtatgaaaaa caaggagaga aatttatgaa
      241 agtagaatta cagattagtg agacttacga ggaggaaaag ctgattgtcc aagcacctca
      301 gccgacagat aaagttcaga aagtcatcga gttcgcagaa aatctggacc aaagagaaaa
      361 aatcaaaggg aaaatcgatg atcaggtcta tctagttgag attggcaaga ttcagcgctt
      421 ctatatcgag aatcggaagg ttctagcaga aaccgcgagt cagacctaca gcattgattt
      481 gcgactctat caggttctta aactcttgcc aaccaatttt atccaaattt cccaatcaga
      541 aatcatcaat atcgattcca tctctcacct caagctcacg ccaaacggtc tggtagaaat
      601 tttcttgaaa aacgaaagct tcacctactc ttcacgccgt tatctaaaaa ccatcaagga
      661 gaaattagaa ctatgaaaaa acaagtattt cacgatgcag ctgccggtgt tcttatcggc
      721 ctcatcctct ctatcctctt ttcactcatt tatgcaccaa atacctacgc accactaaat
      781 ccctactctc tcatcggcca agtgatggat cagcatcagg ttcacggtgc cttggtcttg
      841 ctctactgca cacttatctg ggcagccatt ggtatgctct tcaactttgg caatcgctta
      901 ttcagccgtg actggagcat gcttcgtgcc actctgactc atttcttcct tatgctggct
      961 ggctttgtcc cactagcaac tcttgctggt tggttccctt tccactggat tttctacctc
     1021 cagctcatta tcgagtttgc gattgtctat ctcatcatct gggctattct ctataaaaga
     1081 gaggctaaaa aagtagatca catcaatcaa ctcttggagc atagaaaata ggaacctcat
     1141 aataagcaaa tccgagaaag aaacttctcg gtttttcttt atcctgctgc accttactat
     1201 agt
//
LOCUS       DAVBCG010000203         1190 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVBCG010000203 DAVBCG010000000
VERSION     DAVBCG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.203"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            239..>1190
                     /locus_tag="VQ495_002028"
     CDS             239..>1190
                     /locus_tag="VQ495_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2531937.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          413 a          210 c          249 g          318 t
ORIGIN      
        1 agccacctta ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt
       61 catttgataa caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt
      121 ttagctgaaa aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata
      181 agaaaacacc tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat
      241 gcaagaacat tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga
      301 gcggtggaag aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcgcc
      361 tcaaacgatt cataatgaag tcaaaagagg tacaacttta caacaagtga gaaaagggct
      421 atacaaaaag gtctattctg ccgattacgc acaaactgtt taccaattca atcgaaaacg
      481 gtcggtgaaa aagttaattt taacaaagga aatcagagag aagatcttac actatcataa
      541 gcaaaaattt tcgcctgaaa tgatggttaa caagaagcaa gtgaaagttg gtatttcaac
      601 catctactac tggtttcata atggtcattt aggattgacg aaggccgaca tgctttatcc
      661 cagaaaaagg aaaggtgtca agaagcaagc tagtccgaac tttaagccgg caggtaaatc
      721 tatcgaagaa cgtcctgacg ttattaatct tcgcttggaa aatggtcatt atgaaattga
      781 taccgtccta ctgactaaga taaaaaatta ttgcctgtta gtcttaaccg accggcggag
      841 cagacaccaa attataaggt taattccaaa taaaactgct gaatctgtca atcaggcgct
      901 tacgttacta ttaggggagc atcgtattct gtccattact gcagataatg gttcggagtt
      961 caaacgattg tctgaggtat ttcctgagga acatatctac tacgcacatg cttactcttc
     1021 atgggagaga ggttcaaatg aaaatcataa tcgattaatt cggagatggt tacctaaagg
     1081 aaccaagaaa acgactccga aagaagtagc ttttatcgaa aattggatta acaactaccc
     1141 taaaaaatgc ttggactaca agtcgccaag tgaatttctt ttgggtggct
//
LOCUS       DAVBCG010000204         1114 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVBCG010000204 DAVBCG010000000
VERSION     DAVBCG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.204"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(452..>1114)
                     /locus_tag="VQ495_002029"
     CDS             complement(452..>1114)
                     /locus_tag="VQ495_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531938.1"
                     /translation="EIIRKVQEPEFWKEVNLSDLEKIRLAIRDLLQFLDKTDRKPYYV
                     NFEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKLYHNKKLTSDDMLA
                     LEKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSKFLSDENLNARQIS
                     FVKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNIVQIIELINNRAGE
                     AA"
BASE COUNT          369 a          202 c          197 g          346 t
ORIGIN      
        1 tcctgttcct tatgaaccac ctagaagaaa agcgtcgtta atgatgcatt aaagaacagt
       61 cgagatgaaa aaatcgtgtg acgcaccaag ggaggagtct gcccttttga ggaaatctag
      121 cgaggaaaaa cgatactgga acagcagaaa gtaaaactga cctcatgagg aggaagaaag
      181 tggctcatga ggtcaggggt tttgtaagtt acatctagtt gagagaggta tgaatgattt
      241 gggttaaaat caaagtagct cttttgttaa taactcgatc aacaaacatc gtggtacaag
      301 tatctacttt gaaatttatc aaccacttaa caactgatac tgtattctag gaaaacgatg
      361 acattcttcc taataaaact tctcatatat agcataaatt tctactcttt ttaattttaa
      421 atgagtctca gcatggcaat cactttagaa tttaagccgc ttctccagct cgattattga
      481 taagttcaat gatttgaaca atattacgaa gtactggtag ttggtgttgg aagagtagtt
      541 gaacagaacc ataagattta aacggctctt gcgttaacac tttcgtctct aaaaaaccat
      601 tttctacaat gtagtctaca atcaatttta caaatgaaat ctgcctggca ttaagattct
      661 catccgacaa aaatttagag aaaatacgat tggcagactc tctatctaag ccaataatct
      721 cacgaaccaa tctcggaatt gccttatttt cataatgact ttggtagtct gctttactac
      781 ctaatttttc ccaaagcaat ttttcaagtg caagcatatc atcagatgtc aactttttat
      841 tatggtatag cttagaaatg gactcctcat ccagatgagt tttcaaataa tgctcaactt
      901 tttcattgta agaccgaaga tcgttgacct gcaaaaatgc tgtggtctcg tgaacagtgg
      961 agagtatacg atcttcaaag ttaacatagt agggtttacg gtctgtttta tccaaaaact
     1021 gtaataaatc tcgaatagca agacgaattt tttccaaatc agacaagtta acttctttcc
     1081 aaaattcagg ctcctgtact ttcctgataa tttc
//
LOCUS       DAVBCG010000205         1105 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVBCG010000205 DAVBCG010000000
VERSION     DAVBCG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.205"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(53..721)
                     /locus_tag="VQ495_002030"
     CDS             complement(53..721)
                     /locus_tag="VQ495_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2531939.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(936..1024)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          368 a          239 c          174 g          324 t
ORIGIN      
        1 agattttcga agagtcttac ctcatcaaat ttgtaaatat catgagcctt ctctagacat
       61 cgtaaccaat atcaaaaaaa gctaattcta aagcgactgc ttgattccag cgttgctgaa
      121 gttctgtcaa atcttctcga tttttatcga cacgattgag ttcgtcaacc agaaattgaa
      181 cccactctgc aaagaaagga cctctgtgga gattgatcca ttccgaatga atatagactt
      241 caggtaaagc caaatcttta gaaccccagt ctaaatagag accttctgca atgaccagca
      301 tgaccaaaag atgggcatag tctgatgaag ccaccgccga atacattaga tcctgaaagg
      361 cttttgttac agggtgcaaa gtcacttcta gatagtcatt ctctgctact tttaactctt
      421 taaaagcctt ttggaaataa ccatcttcat ctgcttcaag aaagcctagt tgcttggcaa
      481 aacgaagctt ggattcaagt ttatctgcgt gagctacgca ggcacccagc atggataaga
      541 aggcatcaaa gaagtgataa tcttgaatca gatagtcctt taagacctta ttctcaattg
      601 tccccgcaaa aagttcctta acaaaacgat gattgattgc agcctgccaa tccttctgac
      661 tgctttttaa taattctcca acagtcaaac ctggctgaaa tgcatagtct tgtgtttcca
      721 tatttacttc tcctctcttt acttgttagt aattaataaa acaccaagaa atatcaagca
      781 aaatcgtaat tccacttgat ccttttaaag cacatcgaga gcatttgcag agagctaact
      841 aaacaagcct atccagttta tataaacaaa aaactccaat tacaatcaag aattagagtt
      901 gacttacaag attagatcgt tcatttcgcc atacgaaaaa acgtttcaca tttcccttcg
      961 ccagtcttaa ctgtatcagg ttcaatgggt attatctcag cctaaagcac cccaaatgtc
     1021 tctattattt aattactgaa ccagtatagc aaaaaatgaa agccctagca agatatttga
     1081 ccgaaaaata tctttatata taata
//
LOCUS       DAVBCG010000206         1095 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVBCG010000206 DAVBCG010000000
VERSION     DAVBCG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.206"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..684
                     /gene="queA"
                     /locus_tag="VQ495_002031"
     CDS             <1..684
                     /gene="queA"
                     /locus_tag="VQ495_002031"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2531940.1"
                     /translation="SAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLD
                     DRERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVD
                     NLDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADS
                     GWTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFF
                     SFGDSMFIY"
BASE COUNT          305 a          259 c          200 g          331 t
ORIGIN      
        1 agcgctgtcg ttacagaaga attgacccac gggggacgca ttgtccgctt tgaataccaa
       61 ggaattttcc tagaagtctt ggaaagtctg ggagaaatgc ctctgccacc ttatatccac
      121 gaaaaattag atgaccgtga acgttatcaa accgtctacg ccaaggaaag cggctctgct
      181 gcagcaccga ctgctggtct tcacttcacc aaagaactgc tggcagaaat ccaagctaag
      241 ggtgttcatc tagtctatct gactctccat gtcggactcg gaacctttag acctgtttct
      301 gtggataatc tggacgaaca cgaaatgcat tcagagttct atcaactttc tgaggaagct
      361 gctgccaccc ttcgctctgt caaagaaaat ggtggtcgcg tcatcgctgt cggaaccact
      421 tctatccgca ccttggaaac tattggttcc aagtttgatg ggcaaatcca agcagattct
      481 ggttggacca atatctttat caaacctggg tatgagtgga aggtcgtgga tgccttctca
      541 accaacttcc atctgccaaa atcaactctg gtcatgttgg tttctgcctt tgcaggccgt
      601 gaattagtct tagatgccta ccaccatgcc atccaagaac actaccgctt cttcagtttt
      661 ggtgactcca tgtttattta ttgagaaaga atttctctaa atcttctaat accaataaat
      721 cgctaagata ttatttcaaa gaacatctac aattgaaact ctagctagct gtagaagacg
      781 cctagtacat tgaaattaaa atgcttcccc ctagcttcga aaatattgcc atagattgcg
      841 ttgactctcc aaattgattc atctatattt tatttcagct tcctatattt tcttcgctgt
      901 ttgtaaatca aaatgcaaga cacatgagta gcaccatatt tgttactctt atctgtcctc
      961 tcaagagact attatgagtt atttcagaat cattcactac tttgaccctg actctcctta
     1021 gtctcaaaat caaagactta tactcttcaa aaatctcttc aaactgcgtc aacgtcacct
     1081 tggattatat atgtg
//
LOCUS       DAVBCG010000207         1089 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVBCG010000207 DAVBCG010000000
VERSION     DAVBCG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.207"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            42..>1089
                     /gene="dinB"
                     /locus_tag="VQ495_002032"
     CDS             42..>1089
                     /gene="dinB"
                     /locus_tag="VQ495_002032"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2531941.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGIT"
BASE COUNT          351 a          212 c          231 g          295 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaaa aggtgctagg catgttgatt tttcctttgt
       61 taaatgattt gtcaagaaaa atcatccata ttgacatgga tgcctttttt gctgcagtgg
      121 aaatcaggga taatcctaaa ctcagaggaa aacctgtcat tattggaagc gaccctcggc
      181 aaacaggtgg acggggagtc gtttctacct gtagttatga ggcaagagct tttggtgtcc
      241 attctgccat gagttccaag gaagcttatg aacgttgtcc ccaggctgtc tttatctcag
      301 ggaattatga gaaatacaag tctgtgggac tccagattcg agctattttt aagcgctata
      361 cagatttgat tgaacccatg agcattgacg aagcctattt ggatgtgaca gaaaataaac
      421 tcggtatcaa gtcagcggtc aaaattgctc gcctcattca aaaagatatc tggcaagaac
      481 tccatctaac tgcttccgca ggcgtttctt acaacaaatt cttagctaaa atggcgagtg
      541 attatcaaaa accacatggt ttgacagtga ttctacctga acaggctgag gattttctca
      601 aacaaatgga tatttccaaa tttcatggag taggaaaaaa gacagtagaa cgtcttcatc
      661 aaatgggcgt ttttactggt gctgatttac ttgaagttcc tgaggtaacc ctaatagacc
      721 gttttggtag actaggctat gatctgtatc gaaaggctcg tggcattcac aactctccag
      781 tcaaatccaa tcgcatccgt aaatcaatcg gcaaggagaa aacctacggg aagattctcc
      841 ttgctgagga agatatcaaa aaagagctga ctcttctatc agaaaaagtc gctctcaatc
      901 tacatcaaca agaaaaagct ggaaaaattg tcattttgaa aatccgctac gaggactttt
      961 caactcttac caaacgaaaa agtcttgctc aaaaaacaca agatgctagt cagataagcc
     1021 aaatagccct gcaactctat gaagaattaa gcaagaaaga aagaggtgtc cgcctattgg
     1081 ggattacca
//
LOCUS       DAVBCG010000208         1081 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVBCG010000208 DAVBCG010000000
VERSION     DAVBCG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.208"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>1081
                     /locus_tag="VQ495_002033"
     rRNA            <1..>1081
                     /locus_tag="VQ495_002033"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          315 a          213 c          325 g          228 t
ORIGIN      
        1 gggacacgtg aaatcccgtc ggaatctggg aggaccatct cccaacccta aatactccct
       61 agtgaccgat agtgaaccag taccgtgagg gaaaggtgaa aagcaccccg ggaggggagt
      121 gaaatagaac ctgaaaccgt gtgcctacaa caagttcgag cccgttaatg ggtgagagcg
      181 tgccttttgt agaatgaacc ggcgagttac gttatgatgc gaggttaagt tgaagagacg
      241 gagccgtagg gaaaccgagt ctgaataggg cgccttagta tcatgacgta gacccgaaac
      301 catgtgacct acccatgagc aggttgaagg tgcggtaaga cgcactggag gaccgaacca
      361 gggcacgttg aaaagtgctt ggatgacttg tgggtagcgg agaaattcca aacgaacttg
      421 gagatagctg gttctctccg aaatagcttt agggctagcg tcgacattag agattcttgg
      481 aggtagagca ctgtttgggt gaggggtcca tcccggatta ccaatctcag ataaactccg
      541 aatgccaatg aattatggtc ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg
      601 gaaacagccc agaccaccag ctaaggtccc aaaataattg ttaagtggaa aaggatgtgg
      661 ggttgcacag acaactagga tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa
      721 tagctcacta gtcgagtgac cctgcgccga aaatgtaccg gggctaaaac aatttaccga
      781 agctgtggat acctttatag gtatggtagg agagcgttct atgtgtgatg aaggtatacc
      841 gtgaggagtg ctggaacgca tagaagtgag aatgccggta tgagtagcga aagacaggtg
      901 agaatcctgt ccaccgtaag actaaggttt ccaggggaag gctcgtccgc cctgggttag
      961 tcgggaccta aggagagacc gaaaggtgta tccgatggac aacaggttga tattcctgta
     1021 ctagagtatg tagtgatgga gggacgcagt aggctaacta aagcagacga ttggaagagt
     1081 c
//
LOCUS       DAVBCG010000209         1054 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVBCG010000209 DAVBCG010000000
VERSION     DAVBCG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.209"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..555
                     /locus_tag="VQ495_002034"
     CDS             <1..555
                     /locus_tag="VQ495_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2531942.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(503..1048)
                     /locus_tag="VQ495_002035"
     CDS             complement(503..1048)
                     /locus_tag="VQ495_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2531943.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          165 c          153 g          356 t
ORIGIN      
        1 aaagacgaaa caacctctta tcctatatat gaaatcccag aagcatggag atatattaaa
       61 tttgctagcc tagttaactt cagaatagga aaaactcctc cacgcagtga agctactttt
      121 tggggaactg aaataccttg ggtatctata tcagatatgc ctatatctgg ttatgtaact
      181 aatacgagag aatctatttc taaattagca ttaaaatcaa agaaaataga catttcacct
      241 aaaggaactt tattaatgag ttttaaatta tctattggaa aagttgccat attggatatt
      301 cctgccactc ataatgaagc cattatatcg atattcccct atgctaataa agaaaatatt
      361 atcagagatt acttaatgat atttttgcca cttatctcta ctttaggtga ttcaaaagat
      421 gctataaaag ggaaaacatt aaatagtacc agtatctccg aattattaat ccctatttct
      481 aatcatgaag agatgaaaag aatcatttcc aaagttgatt tactttttca aaaagtttct
      541 caactttttg agtaataagt tcctgttcct caaaaggagc taacggaatt aatagctcgc
      601 ttagtgtagt tttaggaata ttatataaag cttgacctga tagtttagtt attgctttca
      661 attgtttata aaataacgga gaggacaagt taaataatag aaattttgaa ataatctctg
      721 aactttcgaa tggtgttaat tggaaaataa atccaccagc cacaacacca tcatagtctt
      781 tatcgattct tgcaaacttt ccaatatgtt ctaaagaggt tgatacaggt gttattagct
      841 gattatgttt taaataaact tgctcagagg agatgaattg tgtatcaatg tagtaatcat
      901 tatccaacag agaaaattct aaaggcttaa tattaccacc acgtataatt ctaacacctt
      961 tattattaat gcttaaatcg cccttcttgt aagaaagacc tgtatttatt gaaaaaatat
     1021 cttttatttt tataacaacc caattcatag gtat
//
LOCUS       DAVBCG010000210         1035 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVBCG010000210 DAVBCG010000000
VERSION     DAVBCG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.210"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ495_002036"
     CDS             complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQ495_002036"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2531944.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSL"
BASE COUNT          270 a          226 c          183 g          356 t
ORIGIN      
        1 caatgagaaa tagtttatcc atcctcgaat agacaaattc agttgctcaa tacgtcttgt
       61 taggtctata ctccatttcc tctgtgttag tttcttcaat ttaagcttaa atctccaaac
      121 actatcttga tgtggacgac ttttccaacc atctgatgat ttccagaacc cgaaacctag
      181 atatttcaac tctcttggtc tggtaatctt agtcttggtc atgtttactt tcagacctag
      241 ccgtttctca ataaaacgac tgactgaata catcacacgc ttagcggctg cctcgcttcc
      301 gaccgtaatc acacaatcgt ctgcgtagcg aacaaatcga agccctctct tttctaattc
      361 cttgtccaat tcattaagca tgatattgga taagagagga gataaatttc ctccctgtgg
      421 cgtgccaact agcgttttat aacgttgacc gttaatgata acacctgaat gaagatattt
      481 acgaatcaag gattccgtat ctccgtcttc gataatgtta tgtactaagg acatcaatct
      541 atcttgagga actgtatcga aaaatttctc taggtctatg tccactatcc actcatagcc
      601 gtcatttaag tactctaaga gcttcatgat ggctttttca catgacctat ttggtctgaa
      661 tccatagctc gtatctgaga aatggggttc acaaataggg ctaataactt gaacaatggc
      721 ctgttgaatc attctatcca taactgttgg aattcctagt tggcggatgc ctccgtttgg
      781 tttgggaatc tcaactctaa gaactggttg aggcttatat tttctctgtt ttatcagttc
      841 cttagtcagg cgccagtttt gtctgagata gccatccatc tcttcgatag taattccatc
      901 aatcccagct gagcctttat tagattttac ttgattgtag gcttcaagca tattgtcgcg
      961 tgataatatt ttatctagca attttgacat gtgcctattc ctttcttgtt ttggtgcctg
     1021 agggacatct tatat
//
LOCUS       DAVBCG010000211         1020 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVBCG010000211 DAVBCG010000000
VERSION     DAVBCG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.211"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..384
                     /locus_tag="VQ495_002037"
     rRNA            <1..384
                     /locus_tag="VQ495_002037"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            436..508
                     /locus_tag="VQ495_002038"
     tRNA            436..508
                     /locus_tag="VQ495_002038"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:469..471,aa:Ala,seq:tgc)
     gene            633..>1020
                     /locus_tag="VQ495_002039"
     rRNA            633..>1020
                     /locus_tag="VQ495_002039"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          300 a          208 c          290 g          222 t
ORIGIN      
        1 tgccggtaat aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc
       61 tgggctacac acgtgctaca atggctggta caacgagtcg caagccggtg acggcaagct
      121 aatctcttaa agccagtctc agttcggatt gtaggctgca actcgcctac atgaagtcgg
      181 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca
      241 ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac cgtaaggagc
      301 cagccgccta aggtgggata gatgattggg gtgaagtcgt aacaaggtag ccgtatcgga
      361 aggtgcggct ggatcacctc ctttctaagg ataaggaact gcgcattggt cttgtttagt
      421 cttgagaggt cttgtggggc cttagctcag ctgggagagc gcctgctttg cacgcaggag
      481 gtcagcggtt cgatcccgct aggctccatt ggtgagagat caccaagtaa tgcacattga
      541 aaattgaata tctatatcaa atagtaacaa gaaaataaac cgaaaacgct gtagtattaa
      601 taagagttta tgactgaaag gtcagaaaat aaggttaagt taataagggc gcacggtgga
      661 tgccttggca ctaggagccg acgaaggacg tgacaaacga cgatatgcct tgggtagctg
      721 taagtaagcg atgatccagg gatttccgaa tgggggaacc caacaggtaa tacctgttac
      781 ccacatctgt taaggatgtg aggaggaaga cgcagtgaac tgaaacatct aagtagctgc
      841 aggaagagaa agcaaaagcg attgccttag tagcggcgag cgaaacggca gaagggcaaa
      901 ccgaagagtt tactcttcgg ggttgtagga ctgcaatgtg gactcaaaga ttatagaaga
      961 atgatttggg aagatcagcc aaagagagta atagcctcgt atttaaaata gtctttgtac
//
LOCUS       DAVBCG010000212          953 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVBCG010000212 DAVBCG010000000
VERSION     DAVBCG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.212"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..249)
                     /locus_tag="VQ495_002040"
     CDS             complement(<1..249)
                     /locus_tag="VQ495_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2531945.1"
                     /translation="MVSKYLLLAVIFSDLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVA"
     gene            complement(239..895)
                     /locus_tag="VQ495_002041"
     CDS             complement(239..895)
                     /locus_tag="VQ495_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2531946.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
BASE COUNT          324 a          246 c          189 g          194 t
ORIGIN      
        1 ggctacccaa gctgttggaa agacggctaa gaagtctagc catttaattt gaggaaggct
       61 cccaacctta cctgtcacta cgcttgaaag aatcaaggca aagataatgg aaacgggcaa
      121 gaacttcaaa aaacgctcaa cgattgcagg caagccctta tacttgacta agatgaaggg
      181 aatcatacgg agaatccaag tcaccaagtc agagaaaata actgctaata aaagatactt
      241 actgaccatc taaaaccacc cccatgctac aaccaagtag cgtcgcaaac agaacagcta
      301 gtgactgaga caccactgtc aagagcaaaa agaaggacac cgcaacaact gctaggataa
      361 tgagcagatt gcggacagga atccgtcttt gcataatctg aaattgcgaa gcaaaaatac
      421 caataaacat cccaaccagg gcaaaatcca agccaaagat ttctggattt ggtagcaggc
      481 cacccagagc cgttccgact actgtcccca caaaccaagc cacatagctg ttaagattgt
      541 ttccgtgcat ccacatagga tttaccttgt ctgtatgggc caattcaccc atcaaaacgc
      601 cataggtctc atctgtcaag atactagaca taccgatatt gtaccaaaga ctggtatgac
      661 ggaaataagt cgatgcgtgt aaactcaaca aaaagagacg caagttgatt agaaaaaccg
      721 tcatagcaat agctgccaca ggagcttgaa ccacaatcag tgccaacatg gcaaactggg
      781 cactcccagc ataaacaaag agactcatca agcccatctc aacaggtgtc acatagggcg
      841 caccgatagt cccacaggcc aggccgatac tgacatagcc aagagccgtt ggcatggctg
      901 cctgcgcccc ctcctaaaat cctttttctt tcatctttct cctcatattg tct
//
LOCUS       DAVBCG010000213          948 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVBCG010000213 DAVBCG010000000
VERSION     DAVBCG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.213"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..337)
                     /locus_tag="VQ495_002042"
     CDS             complement(<1..337)
                     /locus_tag="VQ495_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002202361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531947.1"
                     /translation="MDSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEIANWH
                     IKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGIT
                     LYHHDQCRSG"
     gene            complement(345..650)
                     /locus_tag="VQ495_002043"
     CDS             complement(345..650)
                     /locus_tag="VQ495_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2531948.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
     gene            complement(637..831)
                     /locus_tag="VQ495_002044"
     CDS             complement(637..831)
                     /locus_tag="VQ495_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531949.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
BASE COUNT          259 a          182 c          188 g          319 t
ORIGIN      
        1 aaccacttcg acactgatca tggtggtaaa gcgtaatccc ttgtttctct gatttacctg
       61 acaaaaaagt ccaatagtaa gtcagctgac tatcactttc taagacccga taagaggttt
      121 catctgcatg aagaagaggt tgttctaaca acttttctcg taaaaggtta taaagggact
      181 ctaaatagta ttgactcgcc ttgatatgcc aattagcaat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattagg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaagctg agtcaatagt ctcgttataa aggggcttta
      361 ggaataggag ctttcacaat tttatcactc ggatttttat cactgcatgc ttggcatttg
      421 taagcgtgtt ggatatgatc tatgcgtttt aattgcgcag gaataaagac taattcttgt
      481 cgttgaaggg ttgctccaat ctcttttaga tctccctgac aatcagagca aatgctctct
      541 tctacttgat gatgaacttc ttctgaatca aattgggcaa gaagagcttg gcgtttccct
      601 ttagatttct tacgtttgta ggtaatttcc tctgtttcaa ctgggaacat ctccatcttt
      661 ctcagatgga gactcttcct caaaaaggtt gagttgtcca gaggggcaaa cacttttctc
      721 agaggatttt ccatagagct tttgagtcag ataagccact tgttcacgaa gaagggcaag
      781 tgcattggtg agactatcaa ttgtagcact ctgttgttga atgattttca atcttcttcc
      841 atgggcttcc cctccttgtt ttctaccttt attatactaa aaagaaagtc ctcatttcaa
      901 tagaaatcac gactttctga tggatttatt tttggagtga tagaaaag
//
LOCUS       DAVBCG010000214          938 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVBCG010000214 DAVBCG010000000
VERSION     DAVBCG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.214"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..763
                     /locus_tag="VQ495_002045"
     CDS             <1..763
                     /locus_tag="VQ495_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2531950.1"
                     /translation="CSGGAKKEGQAASKKEIIVATNSSPKPFNYEENGELTGYEIEVV
                     RAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAVNNISYTKERAEKYLYAAPTVKDP
                     NVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQLEDYNKQHSDNPTILNYTKANLQQ
                     IMGHLSDGQFDYKISDKITVETVIKNQGLDNLKVIDLPSDQQPYVYPLLAKGQDELKT
                     FVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK"
     gene            804..881
                     /locus_tag="VQ495_002046"
                     /pseudo
     CDS             804..881
                     /locus_tag="VQ495_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
BASE COUNT          320 a          186 c          179 g          253 t
ORIGIN      
        1 ttgctccggt ggtgctaaga aagaaggaca agcagctagc aagaaagaaa ttattgttgc
       61 aactaattct tcaccaaaac cattcaacta tgaagaaaat ggcgaattga ctggttacga
      121 gattgaagtc gttcgcgcta tctttaaaga ctctgataag tatgatgtca agtttgagaa
      181 gacagagtgg tcaggtgtct ttgcgggtct tgacgctgac cgttaccaaa tggctgttaa
      241 caacatcagc tacactaaag aacgtgcgga gaaatacctt tatgcagccc caactgttaa
      301 agaccctaac gttcttgtag tcaagaaaga cgattctagc atcaaatcgc ttgatgatgt
      361 tggtggaaaa tcaacagaag ttgttcaagg aacaacatct gctaaacagt tagaagacta
      421 caacaaacaa cactcagata atccaactat ccttaactat actaaagcaa acttacaaca
      481 aatcatgggc cacttgagcg atggtcagtt tgactacaag atttctgata aaatcactgt
      541 tgaaacagtc atcaagaacc aaggtttgga caacttgaaa gtcatcgatc ttccaagcga
      601 ccaacaacct tacgtttacc cacttcttgc taaaggtcaa gatgagttga aaacatttgt
      661 agacaaacgt atccaagaac tctacaaaga cggaactctt gaaaaattgt ctaaacaatt
      721 cttcggagac acttacctac cggcagaagc tgatattaaa taatttcttg aaatcatcca
      781 ttctgagtaa gatgggtgat ttttcagttc gctaccgcaa tctagtcgga acagttctct
      841 ttggagtggt cttgattgcc atcttgcgtt tggtctttta aattacccat aaaaaaaact
      901 tatcacagag atagatatca tataatggcg taaatgct
//
LOCUS       DAVBCG010000215          923 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVBCG010000215 DAVBCG010000000
VERSION     DAVBCG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.215"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..>923)
                     /locus_tag="VQ495_002047"
     CDS             complement(<1..>923)
                     /locus_tag="VQ495_002047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531951.1"
                     /translation="VDADSDAEVLADAEALVDAEAEALVLAEAEALVDAEAEALVLAE
                     AEALVDAEAEALVLAEAEALVDAEAEALVDAEAEALVEAEAEALVDAEADALVLAEAE
                     ALVLAEAEALVEADSEAEVLVDADSDAEVLAEAEALVDAEAEALVDAEAEALVEADSE
                     AEVLVLAEAEALVDADSDAEVLAEADALVDAEAEALVDADSDAEILAEAEALVLAEVD
                     ALVEADSDADVLALVDADVLALVDADVLADVLVLVDADVLAEAEALVLAEADALVLAE
                     VDADSDALVDAEADALVDAEADALVDAEAEAL"
BASE COUNT          231 a          316 c          202 g          174 t
ORIGIN      
        1 agtgcctcgg cttccgcatc aacgagtgcg tcagcctcag cgtcaaccag tgcgtcagct
       61 tccgcatcaa cgagtgcgtc tgaatcggca tcaacctcag caagtacaag tgcatcggct
      121 tcagcaagca ccagtgcttc ggcttcagca agcacgtcag catcaacaag cacaagtaca
      181 tcggcaagca cgtcagcatc gacaagtgca agcacatcgg cgtcaacaag tgcaagtacg
      241 tcagcatctg aatcggcctc aaccagtgcg tcgacttcag caagtacaag cgcctcagcc
      301 tcagcaagta tctcagcgtc tgaatcggca tcaaccagcg cctcagcctc agcatcaaca
      361 agtgcatcag cttcagcaag cacctcagcg tctgaatcgg catcaacgag tgcctcagct
      421 tcagcaagca ccagtacctc agcttctgaa tcggcctcaa ccagtgcctc agcttcagcg
      481 tcaaccagtg cctcagcttc tgcatcaacc agcgcctcgg cctcagcaag cacctcagca
      541 tctgaatccg catcaacgag tacctcagct tctgaatcgg cctcaacaag tgcctcggcc
      601 tcagcaagca caagcgcctc agcttcagca agtaccagtg cgtcagcttc cgcatcaacg
      661 agtgcttcgg cttcagcctc aacgagtgcc tcagcttcag cgtcaaccag tgcttcagcc
      721 tcagcatcaa cgagtgcttc ggcttcagca agtaccagtg cttcggcctc agcatcgaca
      781 agtgcctcag cttcagcaag tactagcgcc tcagcctcag catcaaccag tgcctcggct
      841 tcagcaagta ctagcgcctc agcttcagcg tcaaccagtg cctcagcatc agcaagcacc
      901 tcagcgtctg aatcggcatc aac
//
LOCUS       DAVBCG010000216          902 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVBCG010000216 DAVBCG010000000
VERSION     DAVBCG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.216"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            122..590
                     /locus_tag="VQ495_002048"
                     /pseudo
     CDS             122..590
                     /locus_tag="VQ495_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          331 a          135 c          166 g          270 t
ORIGIN      
        1 gagaagagga ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg
       61 gaaagtacac tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt
      121 tatgcaaatt ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga
      181 agttcattta gacgtaagca ttgaagataa tcagggaggt agtgggtata atttcagttc
      241 tgtttcaagt agctcacaaa cagctaaata tgaaggaact ggttataata acaattcatc
      301 attatatata acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa
      361 taatgttgat aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa
      421 tgctaaagat aatgctggaa actggacaag tgcttcgaat aaagacaagt gcttcgaata
      481 aaaaagaaat aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag
      541 tgaaaaatcc tgataatata aaagatacag aaaaaaatgc cattattgaa aaattgaaag
      601 aggcaaataa aaatcatcca gcaggagctc caacctttgc taaaggtgaa ggagagcatg
      661 caaatgatat tgtagcaact tattcagatg gtacaactta ttatgtaccg ttaaatgatg
      721 tgacaaaata tgcgaggtag tggctgtacc actcacttat tcacctcccc gtgatttgta
      781 gtagtgatag gctttctcac tattattata aaacaaaata aagatcacaa cactttttca
      841 ttctgtgttg tgccttgagt gaaacgaaag gaatgaatta taaatatgaa aagtatagtc
      901 ac
//
LOCUS       DAVBCG010000217          899 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVBCG010000217 DAVBCG010000000
VERSION     DAVBCG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.217"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(385..783)
                     /locus_tag="VQ495_002049"
     CDS             complement(385..783)
                     /locus_tag="VQ495_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2531952.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLQIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          281 a          177 c          122 g          319 t
ORIGIN      
        1 tttcattatt tccattttct ttcggatttc actctattct gaaaaacttg tgtatattta
       61 ctgaagctag caagtcttac ctgtaaattt aatgaaagca acacaaaatc ctagagggga
      121 atctcggatt aatgtcaatt taaaaagata gagagttttt agtttaaata aattgtttta
      181 aaatatcaac aacatcactt cttttcttaa cctgataagc cttgattcct aattttggag
      241 ctacgattat attgtcctca atatcgtcta gaaagacaca atttctaggt tataactggt
      301 atttatcgat agttactcat atacatcagt ccacctccat acttatgtgc gagcctctct
      361 ttgtattata cctccatact caccttacag attcttttgg taataatatc tttgcctaat
      421 gtagagatag tcttgcaaag aaaaaacttc cttgtagcca tgtttctgat aaaagtccgg
      481 tgcctggaac tgataagtat tgacaaaggc aaaacaacaa tttcgattct tagcttcact
      541 ttctgcctgt tgcaatagtt ttgaaccgat tccttgccct cgcagttcct cttttacaaa
      601 caaatactcg atttgtagcc aatttccaaa agtctctgct atcaaacccg caaggagatt
      661 gcccttttca tcttcgacat aaagattaag tggctcactt tcagcctctt ccctttttga
      721 acggttataa acacgaatca gattccctat ttcttgcgat ttatgtgatt ccttattttc
      781 caatctaaag tataatgaaa tgaaataaaa catgcgcaaa tcgattaagg aatttaatct
      841 aatttctaat aatgtcttag aaatcaaagt ttactatttt aacttcaatg cactataca
//
LOCUS       DAVBCG010000218          897 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVBCG010000218 DAVBCG010000000
VERSION     DAVBCG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.218"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            24..869
                     /locus_tag="VQ495_002050"
     CDS             24..869
                     /locus_tag="VQ495_002050"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2531953.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT          227 a          160 c          194 g          316 t
ORIGIN      
        1 aattataata tagggagtaa tctatgtatc ttattgaaat tttaaaatct atcttcttcg
       61 ggattgttga aggaattacg gaatggttgc cgatttccag tacaggtcac ttgattttag
      121 cagaggaatt catccaatac caaaatcaaa atgaagcctt tatgtccatg tttaatgtcg
      181 tgattcagct tggtgctatt ttagcagtta tggtgattta ttttaacaag ctcaatcctt
      241 ttaaaccgac taaggacaaa caggaagttc gtaagacttg gagactatgg ttgaaggtct
      301 tgattgctac tttaccttta cttggtgtct ttaaatttga tgattggttt gatacccact
      361 tccataacat ggtttcagtt gctctcatgt tgattatcta cggggttgcc ttcatctatt
      421 tggaaaagcg caataaagcg cgtgctatcg agccaagtgt aacagagttg gacaagcttc
      481 cttatacgac cgctttctat atcggactct tccaagttct tgctctttta ccagggacta
      541 gccgttcagg tgcaacgatt gtcggtggtt tgttaaatgg aaccagtcgt tcagttgtga
      601 cagaatttac cttctatctt gggattcctg ttatgtttgg agctagtgcc ttaaagattt
      661 tcaaatttgt gaaagccgga gaactcttga gctttgggca attgtttttg ctcttggtcg
      721 cgatgggagt agcttttgcg gtcagcatgg tggctattcg cttcttgacc agctatgtga
      781 aaaaacacga cttcaccctt tttggtaaat accgtatcgt gcttggtagt gttttgctac
      841 tttacagttt tgtccgttta tttgtataag aaaaaccttg aaggggcaac tcttcaa
//
LOCUS       DAVBCG010000219          871 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVBCG010000219 DAVBCG010000000
VERSION     DAVBCG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.219"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            222..>871
                     /locus_tag="VQ495_002051"
     CDS             222..>871
                     /locus_tag="VQ495_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HET2531954.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLL"
BASE COUNT          239 a          195 c          146 g          291 t
ORIGIN      
        1 cattttctcc tgcaattgaa ttcttgctcg gctttttgtt ttctatttct ttaaaacccc
       61 aattattcac ccctaatcta aaaaccatcc agaatccttg ccttagctta gatcctgtat
      121 ggtttctttt ttcacccaat gggtgttttt tactagacaa aaaagaattt cccctttatg
      181 gtataagtgt agaaaaaaac acaaaaagaa aggaaactca catgaacagt ttaccaaatc
      241 atcacttcca aaacaagtct ttttaccaac tatctttcga tggaggtcat ttaacccagt
      301 atggtggtct tatctttttt caggaacttt tttcccagtt gaaactaaaa gagcggattt
      361 ctaagtattt agtaacgaat gaccaacgcc gctactgtcg ttattcggat tcagatatcc
      421 ttgtccagtt cctctttcaa ctgttaacag gctatggaac ggactatgct tgtaaagaat
      481 tgtcagctga tgcctacttt ccaaaattgt tggaaggagg gcagcttgct tcacagccaa
      541 ccttatcccg ttttctttcc agaactgacg aggaaacagt ccatagtttg cgatgcctca
      601 accttgaatt ggtcgaattc tttttacagt ttcaccagct aaaccaactc attgtagata
      661 tcgattctac ccatttcaca acttatggca agcaagaagg tgttgcttat aacgcccact
      721 atcgtgctca tggctatcat cctctttatg ctttcgaggg gaagacaggt tattgtttca
      781 atgcccagct tcgtcctggt aatcgttatt gttctgaaga ggcagacagc tttatcacac
      841 ctgttttaga acggtttaat caacttctct t
//
LOCUS       DAVBCG010000220          834 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVBCG010000220 DAVBCG010000000
VERSION     DAVBCG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.220"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..>834)
                     /locus_tag="VQ495_002052"
     rRNA            complement(<1..>834)
                     /locus_tag="VQ495_002052"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          178 a          267 c          176 g          213 t
ORIGIN      
        1 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
       61 gcaccacctg tcacctctgt cccgaaggaa aactctatct ctagagcggt cagagggatg
      121 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      181 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      241 cttaatgcgt tagctacggc actaaacccc ggaaagggtc taacacctag cactcatcgt
      301 ttacagcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      361 cgtcagttac aagccagaga gccgctttcg ccaccggtgt tcctccatat atctacgcat
      421 ttcaccgcta cacatggaat tccactctcc cctcttgcac tcaagttaaa cagtttccaa
      481 agcctactat ggttaagcca cagcctttaa cttcagactt atctaaccgc ctgcgctcgc
      541 tttacgccca ataaatccgg acaacgctcg ggacctacgt attaccgcgg ctgctggcac
      601 gtagttagcc gtccctttct ggtaagatac cgtcacagtg tgaactttcc actctcacac
      661 tcgttcttct cttacaacag agctttacga tccgaaaacc ttcttcactc acgcggcgtt
      721 gctcggtcag acttccgtcc attgccgaag attccctact gctgcctccc gtaggagtct
      781 gggccgtgtc tcagtcccag tgtggccgat caccctctca ggtcggctat gtat
//
LOCUS       DAVBCG010000221          750 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVBCG010000221 DAVBCG010000000
VERSION     DAVBCG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.221"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(177..622)
                     /locus_tag="VQ495_002053"
                     /pseudo
     CDS             complement(177..622)
                     /locus_tag="VQ495_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          207 a          144 c          152 g          247 t
ORIGIN      
        1 ggttgtagat gaaactgacg aagtcagtaa ccatacctac ggcaaggcga cgttgacgcg
       61 gattgaagag attttcgaag agtataaatc ttcctaggat aaagcaaaga gcctgggaca
      121 aaatagttct cgaccacaaa aaagctagag atttccaatt gtggaacttt agctttttaa
      181 ttttgagtcg ttctcttatt gtattttagg aggttattgg ccataagtac caatcccatg
      241 tcaattctca cttgacgctt gcccctcaga ttacatctct tgtaacccaa acaagccttt
      301 atctgcccaa agacaggtga cattctttag ctttcaagtt ttgatagcct tcgttcatat
      361 acagtccctt ttgaggggct gattcaggtt cgtcggcgta gtaaaccttg atttcttgtt
      421 taaagtctgt ctgtgttttc tgatgtttgg tatggtgaaa acgataatac cagccatcag
      481 gatgtgtata gctatcctcc ttgtcattat agtaccaatt cgctaagttt ctagctgact
      541 gtttatagcc tctcttctgt tccttatcaa acatggcata tttaatcaga tggtttacct
      601 ccttttcatc taactaaagg agaagcaata tggctgaacg attttgggag aacttgtcca
      661 ttattttggc tgacagaaat atcagctgga ttgagttaac cagaaaaatg tttgcgggag
      721 agtttcacta tccaagtgaa ttgaatcgtt
//
LOCUS       DAVBCG010000222          718 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVBCG010000222 DAVBCG010000000
VERSION     DAVBCG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.222"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            298..447
                     /locus_tag="VQ495_002054"
     CDS             298..447
                     /locus_tag="VQ495_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HET2531955.1"
                     /translation="MGDQPVQDTETSSALILSHYLDEQDLSEKLKSDLQWFELENKLL
                     NLWEY"
     gene            435..>718
                     /locus_tag="VQ495_002055"
     CDS             435..>718
                     /locus_tag="VQ495_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531956.1"
                     /translation="MGVLGYYEAFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVA"
BASE COUNT          276 a           89 c          140 g          213 t
ORIGIN      
        1 aagagcttgg caacaggctc tttttgtatt gcgtttatta agaaaaacaa aaaacatcga
       61 tatacattta actaaaaatt attgtatgtt atctccctta attaggaatg ataagggaat
      121 aactagaaag atttgtgaat acaaactatt tctgatatac aaaatataca gtaataatga
      181 atgatgggag atgggatgaa agaatttcaa tttgagagaa agccgcgttt ttctttgagg
      241 ccatatgcaa taggagcttg ttaggtcttg ctaggaacga gtttattttt tgctggtatg
      301 ggtgatcagc ctgtacagga tacagaaacg agttcagcac taattttaag tcattatttg
      361 gatgagcagg atttatctga aaagctgaaa tctgatttgc aatggtttga attagaaaac
      421 aagcttttga acttatggga gtattaggtt actatgaagc atttgttcct tatgtttcaa
      481 atcaatacaa aaaccaagct gaagaagaag gcaaaccgct atctgataaa tatattttcg
      541 aaaaaatatt aggaaaaaca tatgcagcct tcaaaaaaga tcaaattaat gagcgtgtcg
      601 agaaattagg taagttaaaa cctattacaa taaattacaa cggaaaatca gaagtaattg
      661 atagtaaaga aaaattacaa gagcttatga ataaagccgt taaagacgaa gtggctca
//
LOCUS       DAVBCG010000223          715 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVBCG010000223 DAVBCG010000000
VERSION     DAVBCG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.223"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            143..676
                     /gene="queF"
                     /locus_tag="VQ495_002056"
     CDS             143..676
                     /gene="queF"
                     /locus_tag="VQ495_002056"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2531957.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT          219 a          170 c          132 g          194 t
ORIGIN      
        1 ttatttcatt ttactatata agtagaatat tacgatgttt gggatatgcc atcgctaaaa
       61 aaaaactcga tttggtattt tttagttccc taatgggagc tttttgcgtg ctctgaacat
      121 ttcccatttt ggaaggagta ctatgaaaca tcaatcagcc ttggtcgtct ttagtggcgg
      181 tcaagattca acaacctgcc ttttctggac catgcaacac tatgaaacag tcgaagctgt
      241 cacctttgcc tacggccaac atcatcacct cgaaattcaa attactagag aaatcgctaa
      301 ggaacagggc attcgtcacc atatcctcga tatgtctctg ctgggacaaa tcactgctca
      361 gccagacttt gcgacgattc atatttccta cattcctgac aagctctgtg tcgagtcaaa
      421 atccctcaaa ctatatctat ttagctaccg aaaccacgga gatttccacg aaaactgtat
      481 caacaccatc gggaaagact tggtcaactt gctagaccct cgctatttag aagtctgggg
      541 aaaattcact ccgcgcggtg gcatttcaat cgacccctac tataactacg gtaagcaagg
      601 aactaagtat gagggcttgg cagaacaacg cctcttccaa cacgaccttt atccagagaa
      661 aattgacaac cgctaaagtc atactcaatg aaaatcaaag agcaaactag gaagt
//
LOCUS       DAVBCG010000224          692 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVBCG010000224 DAVBCG010000000
VERSION     DAVBCG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.224"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VQ495_002057"
     tRNA            complement(74..147)
                     /locus_tag="VQ495_002057"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VQ495_002058"
     tRNA            complement(157..242)
                     /locus_tag="VQ495_002058"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VQ495_002059"
     tRNA            complement(250..321)
                     /locus_tag="VQ495_002059"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VQ495_002060"
     tRNA            complement(344..416)
                     /locus_tag="VQ495_002060"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VQ495_002061"
     tRNA            complement(435..516)
                     /locus_tag="VQ495_002061"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VQ495_002062"
     tRNA            complement(522..594)
                     /locus_tag="VQ495_002062"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VQ495_002063"
     tRNA            complement(619..691)
                     /locus_tag="VQ495_002063"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
BASE COUNT          179 a          205 c          149 g          159 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt accttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ct
//
LOCUS       DAVBCG010000225          689 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVBCG010000225 DAVBCG010000000
VERSION     DAVBCG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.225"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(262..573)
                     /locus_tag="VQ495_002064"
     CDS             complement(262..573)
                     /locus_tag="VQ495_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531958.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
BASE COUNT          223 a          128 c          112 g          226 t
ORIGIN      
        1 ccaccacggt tggcatcacg catactctag acgcaattct aatcttttag actttgcata
       61 ataactgtta aacagttgaa aattaaccca gttttaacaa gtagtcaaaa taccagataa
      121 aggaaaaaaa gtctttaaaa tcttaaaaac gcatagtatc aggtattgaa caatcttgat
      181 attatgcgtt ttattgtggg aagatttact tcattttctc ttggaattga gttttctcct
      241 acttcaaaac tagatgattt gctatttaaa atagcgttcc ttatgatttt ggcaacctgg
      301 attaaagcga gaaaaacaaa aggggcaact taagctttct ttatattgct tatatgttag
      361 tagttttaga caaacaccac ataaaatagg cttatcctgt atcaaagata agggatacgg
      421 ctcaaaaggg tgattttcta atgaatcatg acaccgataa caagcatagt actttttaca
      481 aacatagcat tgtaaagcaa taatatccag cttgctatgg taatgaatgc atcttgtttg
      541 cttatccact aagaaaccct gtgcctctat catttccttt tcctttctta agctcgaact
      601 gtgacgctgg ttccatcttc cttataaaga ttgatgagtc cttcttttaa tgcacgaacc
      661 atgtctccag cttgaacagg ttgaggcac
//
LOCUS       DAVBCG010000226          671 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVBCG010000226 DAVBCG010000000
VERSION     DAVBCG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.226"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>671
                     /locus_tag="VQ495_002065"
     CDS             <1..>671
                     /locus_tag="VQ495_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2531959.1"
                     /translation="HVESDGLVFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKR
                     IARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAV
                     RKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDRE
                     FYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIR
                     HPERLG"
BASE COUNT          210 a          148 c          137 g          176 t
ORIGIN      
        1 catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg aaccgccaga
       61 ggtgtagctg tccctcatgg taaccattac cactttatcc cttatgaaca aatgtctgaa
      121 ttggaaaaac gaattgctcg tattattccc cttcgttatc gttcaaacca ttgggtacca
      181 gattcaagac cagaacaacc aagtccacaa tcgactccgg aacctagtcc aagtctgcaa
      241 cctgcaccaa atcctcaacc agctccaagc aatccaattg atgagaaatt ggtcaaagaa
      301 gctgttcgaa aagtaggcga tggttatgtc tttgaggaga atggagtttc tcgttatatc
      361 ccagccaagg atctttcagc agaaacagca gcaggcattg atagcaaact ggccaagcag
      421 gaaagtttat cgcataagct aggagctaag aaaactgacc tcccatctag tgatagagaa
      481 ttttacaata agacttatga cttactagca agaattcacc aagatttact tgataataaa
      541 ggtcgacaag ttgattttga ggctttggat aacctgttgg aacgactcaa ggatgtctca
      601 agtgataaag tcaagttagt ggatgatatt cttgccttct tagctccgat tcgtcatcca
      661 gaacgtttag g
//
LOCUS       DAVBCG010000227          616 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVBCG010000227 DAVBCG010000000
VERSION     DAVBCG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.227"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(194..>616)
                     /locus_tag="VQ495_002066"
     CDS             complement(194..>616)
                     /locus_tag="VQ495_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2531960.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          158 a          121 c           93 g          244 t
ORIGIN      
        1 taaatgatga agttttttct caacttcttt tactttatta aatagttttc taaaccggtt
       61 atttaaccat ttaattaacc agtaaatagt ttactattta aaattacttt tgtcaagttt
      121 ctgttacgtt tttctaaaaa agagaagtaa cacgaaaggt tacttctcca taaaatatgc
      181 tccattcatt ttactactgt ataggagtcg gttgactttc ttttaacaaa gccaagagac
      241 tatctacttc tgctgaaata gtgttattat ctttcgttcc gagaagaaga ctacttttta
      301 gaccagtcaa tgtctccaca gcattttgtc taatactaga atctgttact ttttctagca
      361 aggcctccgc ctctgctatc ttagcgttaa taacagaatg ctctacttga ggaatttcag
      421 cctcatctgt agtatcttca gcttcttcct ctgtctcttc cgtatcagtg cttggtttat
      481 aaagattatc tgctgaagga tttaaaccag cgtgattctc tttttcatca gattcagggt
      541 gagttggctc acttacttca tcatgttcct tatcttcctc aggtttttct gtctgaggtt
      601 tctcctcgtt tggttt
//
LOCUS       DAVBCG010000228          592 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVBCG010000228 DAVBCG010000000
VERSION     DAVBCG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.228"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..175
                     /locus_tag="VQ495_002067"
     CDS             <1..175
                     /locus_tag="VQ495_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2531961.1"
                     /translation="EDKACNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRM
                     NLIAGIINHELGF"
     gene            378..>592
                     /locus_tag="VQ495_002068"
     CDS             378..>592
                     /locus_tag="VQ495_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HET2531962.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAA"
BASE COUNT          217 a           77 c          135 g          163 t
ORIGIN      
        1 tgaagataaa gcctgtaatc atgcgctatc taaggagaga agcaaggttg agaatatctt
       61 tgccaaagta aaaacgttta aaatgttttc aacaacctat cgaaatcatc gtaaacgctt
      121 cggattacga atgaatttga ttgctggtat tatcaatcat gaactaggat tctagttttg
      181 caggaagtct aatagtaaaa aagtgattag aaaacatctt ttttaaaaat agagatgatt
      241 ttgaaacaaa aaagctaatt caagacgttt cgatgccaat tcaagatttg gatgaaaaaa
      301 attaatagat actgttatac taaacttgtc aagtttgtaa caagacaaat attaaaaata
      361 aaaaagaggt attcgttatg aatacaaaaa agatgtcaca atttgaaatt atggatactg
      421 agatgcttgc ttgcgttgaa ggtggcggat gcaattgggg agattttgcc aaagcaggtg
      481 ttggaggagg agcagtacga ggtcttcagc taggaattaa aacaagaaca tggcaaggtg
      541 cagcaactgg tgctgcggga ggagctatac ttggaggtgt ggcctatgca gc
//
LOCUS       DAVBCG010000229          558 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVBCG010000229 DAVBCG010000000
VERSION     DAVBCG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.229"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT          178 a          113 c           74 g          193 t
ORIGIN      
        1 agataggact gacgaagtca gtcacatata tacggcaagg cgacgttgac gtggtttgaa
       61 tttgattttc gaagagtata aactcatctg ataacaacta tcttacccca atcactaaac
      121 aaaaactgga cttccttccc aaatgagcct atctccctcc caactgcact ttttggataa
      181 aaaaataggc agaacaagag tcctgcccac taaaaatatg ctattgaaag gaaattgatt
      241 ttttactgtc accctaggca agcttgaaac aaataaatca ttgatagtaa cacttacgac
      301 atcctattta ttacttcaat ttctaatgtc cctgctattt ctaagctacc tctggtatga
      361 tctattccta gtcagaatag ataacaccgt tctaaattac gattcatagt tactcctttt
      421 ttgactcgtt actataagac tcctctttac atgcgcaaaa taattttgat tcagattcat
      481 catttatagt tttgattatt ttttaacaga tttttctgtt ttcttctccg aagactttaa
      541 aaattttttt ctaattaa
//
LOCUS       DAVBCG010000230          542 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVBCG010000230 DAVBCG010000000
VERSION     DAVBCG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.230"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQ495_002069"
     CDS             84..491
                     /locus_tag="VQ495_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2531963.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
BASE COUNT          189 a          114 c           98 g          141 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tc
//
LOCUS       DAVBCG010000231          536 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVBCG010000231 DAVBCG010000000
VERSION     DAVBCG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.231"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(251..511)
                     /locus_tag="VQ495_002070"
     CDS             complement(251..511)
                     /locus_tag="VQ495_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531964.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT          178 a          103 c           82 g          173 t
ORIGIN      
        1 atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac aaatcaagtg
       61 actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa cgcataatat
      121 caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac ttcattttct
      181 cctgaaattg aagttttttc tagtttccag atatagacga attcaatata gccatctcgc
      241 tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca ttttgatcca
      301 atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct atcacaaagc
      361 ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt tcatctccta
      421 gcaaggaagc tgtcaaacgg gtaatgtcat tcaaatgctt tttaatggac ttacttaaac
      481 cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc ttaaaa
//
LOCUS       DAVBCG010000232          526 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVBCG010000232 DAVBCG010000000
VERSION     DAVBCG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.232"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(238..325)
                     /locus_tag="VQ495_002071"
     tRNA            complement(238..325)
                     /locus_tag="VQ495_002071"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Ser,seq:gct)
     gene            complement(332..405)
                     /locus_tag="VQ495_002072"
     tRNA            complement(332..405)
                     /locus_tag="VQ495_002072"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Ile,seq:gat)
     gene            complement(441..511)
                     /locus_tag="VQ495_002073"
     tRNA            complement(441..511)
                     /locus_tag="VQ495_002073"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Gly,seq:tcc)
BASE COUNT          176 a          119 c           92 g          139 t
ORIGIN      
        1 gcaatgcctt tggttttcga aactttgaaa actttaaaaa acggattttt atcgctttga
       61 acatcaaaaa agaaaggacg aaatttgtcc tttctcgagc ttagcttttc ttcaacccac
      121 tacagttgac aaagagccat aaaaataact tcagattagt aattcaatta agttttacgg
      181 atctgaagct ttattgtctc taaaaataaa aaagagagtt acaaactctc attaaaacgg
      241 agaataaggg attcgaaccc ttgcgccagt tacccgacct aacgatttag caaaccgtcc
      301 tcttcagcct cttgagtaat tctccaatta atgggcacga gtggactcga accaccgacc
      361 tcacgcttat caggcgtgcg ctctaaccac ctgagctacg cgcccaagtt aaaaaacttg
      421 gtaatttgaa caaagttcaa agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa
      481 ggctgtagtt ttaccactaa actacacccg cataaatact atcaat
//
LOCUS       DAVBCG010000233          524 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVBCG010000233 DAVBCG010000000
VERSION     DAVBCG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.233"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            56..355
                     /locus_tag="VQ495_002074"
     CDS             56..355
                     /locus_tag="VQ495_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2531965.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
BASE COUNT          200 a          100 c           80 g          144 t
ORIGIN      
        1 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
       61 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      121 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      181 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      241 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      301 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      361 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
      421 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
      481 caatgaagaa gcaaagatca aactaggcag ctagttacaa gtta
//
LOCUS       DAVBCG010000234          520 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVBCG010000234 DAVBCG010000000
VERSION     DAVBCG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.234"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            1..520
                     /locus_tag="VQ495_002075"
                     /pseudo
     CDS             1..520
                     /locus_tag="VQ495_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          178 a          102 c          111 g          129 t
ORIGIN      
        1 gaaaatcaaa tagagaaatt gcctctctac ttggaaaagc tcctcaaact atccacacta
       61 aaatcaagcg taggacagtc cgaaaatgtc ttggaaaagg gcgcttcaaa gaggtttatt
      121 ctgccgacta tgctcaacag tcttatgaaa ataatcgcaa gcactcggtc aagagatcaa
      181 gcgtgaccaa ggaactaaag gaaaagattc tccactatca taaccaaaaa tttttgcctg
      241 aaatgatggt tatggctaaa ggggttaacg tgggaatttc aaccatttac tattggattc
      301 atcatggaaa attggggtaa agcaagcagg atctgcttta ccctagaaaa ggaaaagtgc
      361 ttaagaaaca ggccagcacc aactttaaac ctgctggtca atccatcgaa cagcggcttg
      421 aagctatcaa ttttcgcttg gagaatgggc attatgagat tgatacggtt ctacttacga
      481 gagcgaaaaa ctactgcttg cttgtcttga cggatcgaaa
//
LOCUS       DAVBCG010000235          514 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVBCG010000235 DAVBCG010000000
VERSION     DAVBCG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.235"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT          167 a           85 c          133 g          129 t
ORIGIN      
        1 gggagaggaa gggatagcga aatcgtggct ctacgaacag gaacgtgata gtaaggcgta
       61 tatagcggat aaggaggctt caaactctaa agtccaaaaa ggtagtcgta acctatatgt
      121 gtaaatcacg agagtaattg aattcggact aaggtttgtg tgaaaaagat aaatctttct
      181 agagtctaaa gactctgcgt cagatttcct attttcactg taacctttta acgtcctcat
      241 atcttgtata aacgaggaaa gatgtacgac ttatcccgtg aggtttcatg agcgctgaaa
      301 gcgtagtaac aacgaatcat gagaagtcag ccgagcccat agtagtgagg aaacttccgt
      361 aatggaagtg gagcgaaggg gtgaatactc aaacagtctg gggagagact gtttgaggtc
      421 tgtcgctaga aagagaaaac gacagatcga agtaatccta cttcacttgt gtctgtaaaa
      481 tgagtggtct gatagaactg gactttgaca cgta
//
LOCUS       DAVBCG010000236          501 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVBCG010000236 DAVBCG010000000
VERSION     DAVBCG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.236"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>501
                     /locus_tag="VQ495_002076"
     CDS             <1..>501
                     /locus_tag="VQ495_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2531966.1"
                     /translation="FGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLNTKN
                     VTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHLRIN
                     SGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQN
                     EFADFVA"
BASE COUNT          190 a           76 c           84 g          151 t
ORIGIN      
        1 gtttggggaa aataaaatat ttgaaagcat tgataactta tttgatatta tagatggtga
       61 taggggcaaa aattatccta aatcagatga gttgtttagt gaggagtact gtttattttt
      121 aaatacaaag aatgttacta aaaacggatt ttcattcgat acaaagcaat ttatcactaa
      181 aacaaaggat aaattacttc gaaaaggcaa gcttgagcgt tatgatatag tcttgacaac
      241 aagaggtact gttggaaatg tagcgtacta cgatgaatta ataaaatata aacatttacg
      301 tataaattca ggtatggtaa tattacgtcc caagacacca aatctaaatc agaaatttat
      361 tatccatgtt ttaaggaata ataattatag tcgagtgata tcaggaagtg ctcagcctca
      421 gttaccaatt acaaaattaa aaaaaatact tctccccctc cccccactag ccctccaaaa
      481 tgagttcgca gactttgtag c
//
LOCUS       DAVBCG010000237          483 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVBCG010000237 DAVBCG010000000
VERSION     DAVBCG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.237"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..364)
                     /locus_tag="VQ495_002077"
     CDS             complement(<1..364)
                     /locus_tag="VQ495_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2531967.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHS"
BASE COUNT          137 a           92 c           97 g          157 t
ORIGIN      
        1 aactatgaaa agctaggtca tctgagaagt aattactatc gacaattgcg atagcgtata
       61 ctagtgcgat ttgtttgtag ctctggtggg tatcaccttc acgttggcga attttttcta
      121 ggttttgatt aacctgactg cacagataag cccataggcg attgatgaaa aaattctgat
      181 gatgaacctg aatttcgatg ataacttgga ttccattatc taactccgcc aagacgtcta
      241 tactagtata gaaatcttgc gctgagtagg gcatggaagg caagacgtga atgttacttc
      301 cctccaaaat cgtcacattt tttgctggca agtccagcat atcgcggata aattgacaaa
      361 tcattagttg ggctgatacc tggatgtctg agaatcctcc ttttcctcct tctattatag
      421 cacattttta aatctaggtt tgctagattc tatgcttcta tctatttatt cggaaaagaa
      481 agc
//
LOCUS       DAVBCG010000238          482 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVBCG010000238 DAVBCG010000000
VERSION     DAVBCG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.238"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            30..>482
                     /locus_tag="VQ495_002078"
     CDS             30..>482
                     /locus_tag="VQ495_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2531968.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVF"
BASE COUNT          178 a           62 c          116 g          126 t
ORIGIN      
        1 gttagactgt atctagaaag gggaaaatta tgattaaaga attgtatgaa gaagtccaag
       61 ggactgtgta taagtgtaga aatgaatatt accttcattt atgggaattg tcggattggg
      121 accaagaagg catgctctgc ttacatgaat tgattagtag agaagaagga ctggtagacg
      181 atattccacg tttaaggaaa tatttcaaaa ccaagtttcg aaatcgaatt ttagactata
      241 tccgtaagca ggaaagtcag aagcgtagat acgataaaga accctatgaa gaagtgggtg
      301 agatcagtca tcgtataagt gaggggggtc tctggctaga tgattattat ctctttcatg
      361 aaacactaag agattataga aacaaacaaa gtaaagagaa acaagaagaa ctagaacgcg
      421 tcttaagcaa tgaacgattt cgagggcgtc aaagagtatt aagagactta cgcattgtgt
      481 tt
//
LOCUS       DAVBCG010000239          442 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVBCG010000239 DAVBCG010000000
VERSION     DAVBCG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.239"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT          148 a           83 c           77 g          134 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga gcagcatttc ctagccaata ggacgctcca aatcacctat tctaaatcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtgtt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta ca
//
LOCUS       DAVBCG010000240          431 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVBCG010000240 DAVBCG010000000
VERSION     DAVBCG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.240"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(105..>431)
                     /locus_tag="VQ495_002079"
     CDS             complement(105..>431)
                     /locus_tag="VQ495_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2531969.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
BASE COUNT           82 a           92 c           75 g          182 t
ORIGIN      
        1 acattttcat gaaaaaaatg agaactagaa ctcacattct gctctcattt ttcgttttcc
       61 cgttctccta tcctgttttt aggagttaga aaatgctgct acctttactt actctccttt
      121 aataaagcca atagtttttc agcttctgcc ataatagtat tgttgtcctg ggtgccaaat
      181 agtaaattat tttttaatcc tgtgagagtc tctttggcat tggacttgat aattggatcc
      241 tggatttttc caagtaaatc ttcagcctct ctcatttttt cttcaacctt ttcagtctcg
      301 acctgaggtt cttctgattc ctctggtgat tcttcttctg gttcctctgt tggttttgga
      361 gactctggtt tctcgctttg cggtttctct tctcgagggg tttcttcctc aggtttttct
      421 gtctgaggtt t
//
LOCUS       DAVBCG010000241          410 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVBCG010000241 DAVBCG010000000
VERSION     DAVBCG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.241"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>410
                     /locus_tag="VQ495_002080"
     CDS             <1..>410
                     /locus_tag="VQ495_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
                     /protein_id="HET2531970.1"
                     /translation="GKKKRHTLKTQIMLDLTTHKVCQMAFSDGHTHDFTLFKESIGQS
                     LPETTLAFVDLGYLGILKFHENTFIPAKNSKNRRLSEDDKQLNKEMSAIRIEIEHFNA
                     KFKTFQIMSVPYRNRRKRFELRAELICAIINYEV"
BASE COUNT          138 a           78 c           76 g          118 t
ORIGIN      
        1 tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga cgacacataa
       61 agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct tcaaagaaag
      121 tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt atttaggcat
      181 cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc gccgcctgag
      241 tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa ttgaacattt
      301 taacgctaaa ttcaagacct tccaaatcat gtcagtccct tatcgtaacc gcagaaaacg
      361 tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
//
LOCUS       DAVBCG010000242          404 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVBCG010000242 DAVBCG010000000
VERSION     DAVBCG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.242"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            9..>404
                     /locus_tag="VQ495_002081"
     CDS             9..>404
                     /locus_tag="VQ495_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HET2531971.1"
                     /translation="MLYVGIDVAKNKHDVTALNVQGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALEDTGHYAFNLLNFLHEQGYKVYTYNPLLIKKFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHNNRQVELKIL"
BASE COUNT          134 a           92 c           60 g          118 t
ORIGIN      
        1 gtactcccat gctttatgtg ggcattgatg tcgctaaaaa taaacacgat gttacagcct
       61 tgaatgttca aggaaaaact gttcttaaac cactcacttt ttcaaataat aaagctggtt
      121 ttgaactctt agatctgtct cttcgacagc tcaaccaaga ctgtctcatc gctcttgaag
      181 atactggaca ttatgccttt aatctactga acttcctgca tgaacaagga tataaagttt
      241 acacctacaa tcctttgctc atcaagaaat ttgccaaatc attatcactt cgcaagacta
      301 aaacggataa gaaagacgct catggcatcg ctcttaaact tctttctgac cccaatcgtg
      361 aacaatttca acacaataat cggcaagtag aactaaaaat actg
//
LOCUS       DAVBCG010000243          386 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVBCG010000243 DAVBCG010000000
VERSION     DAVBCG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.243"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            146..280
                     /gene="rpmH"
                     /locus_tag="VQ495_002082"
     CDS             146..280
                     /gene="rpmH"
                     /locus_tag="VQ495_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2531972.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT          132 a           66 c           83 g          105 t
ORIGIN      
        1 gggcttgcat tcgaaaaagc tggaaacatt tgcctagagc tattgacaaa agtgaaagaa
       61 tcagatagaa tagtaaagta tgtttagtaa ctgatcactt tatagccggc taaacgaata
      121 caaaatctat gaggaggtat tcatcgtgaa acgtacttat caaccaagta aacttcgtcg
      181 tgcgcgcaaa cacggattcc gtaaccgtat gtcaactaaa aatggtcgtc gcgtattggc
      241 agctcgtcgt cgtaaaggac gcaaagtttt ggctgcataa tccaaacgaa ttctatcaaa
      301 aatcagtagg aactcgagtc tactgatttt tatttttgta aaaaagttca gtagatgcaa
      361 atggattcgg aagcgatgtt atagta
//
LOCUS       DAVBCG010000244          372 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVBCG010000244 DAVBCG010000000
VERSION     DAVBCG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.244"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            21..>372
                     /locus_tag="VQ495_002083"
     CDS             21..>372
                     /locus_tag="VQ495_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
                     /protein_id="HET2531973.1"
                     /translation="METVYDKAQKLNSKNFKLLIGVKKETFQLMLEHLNSAYQIQHRK
                     GGRPRSLPMEDQLIMTLRYLRYYPTQRLLAFDFGVGVATVNAIITWVEDTLRASGSFD
                     LDHLEAPSAAVAIDV"
BASE COUNT          106 a           84 c           80 g          102 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt ga
//
LOCUS       DAVBCG010000245          368 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVBCG010000245 DAVBCG010000000
VERSION     DAVBCG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.245"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(43..354)
                     /locus_tag="VQ495_002084"
     CDS             complement(43..354)
                     /locus_tag="VQ495_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531974.1"
                     /translation="MKVPNYRQEEDWARMGLPITRKEISNRHIKTSQYYLEPLYNLLR
                     ERLLTQPLLHADETSYRVLESDSQLTYYWTFLSGKAEKQGITLYHHVLIDLFISYFNP
                     L"
BASE COUNT          114 a           71 c           71 g          112 t
ORIGIN      
        1 ttggaagaca ttctagaaga taggatagat atttctcact atttataatg gattgaaata
       61 agatatgaac aaatcgatta ggacatgatg gtaaagcgta atcccttgtt tctcagcttt
      121 cccagacaaa aaagtccaat agtaagtcag ctgactatca ctctctagca ccctataaga
      181 agtttcatcc gcatgaagta agggctgagt caatagtctc tctcgcaaga ggttataaag
      241 gggctccaaa tagtattgac tcgtcttgat atgccgatta gagatttcct tacgtgtgat
      301 tggtaaaccc atcctagccc aatcttcttc ttggcgataa ttgggtacct tcagattaaa
      361 cttctgat
//
LOCUS       DAVBCG010000246          339 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVBCG010000246 DAVBCG010000000
VERSION     DAVBCG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.246"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT           90 a           81 c           69 g           99 t
ORIGIN      
        1 tagagcctcg atttcgctat cccttcctct gacgattctt atttcctgcg tcagacttaa
       61 aacggtctat ccccagaccg ttttaatccg ctacctcacg atagtcaggc ttgggaatcg
      121 ctattgggtt caccggtagc aggtgcccac ggaggactta cacctcagat gcacgacatg
      181 cccgtcgtac ttaaaatcag cctaaatgat tcaggctgat ttttctattg attcaaaaaa
      241 acggctcttt gtcaactgta gtgggttgaa gaaaagctaa gatcgagaaa ggacaaattt
      301 cgtcctttct tttttgatat tcagagcgat aaaaatccg
//
LOCUS       DAVBCG010000247          330 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVBCG010000247 DAVBCG010000000
VERSION     DAVBCG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.247"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..>330)
                     /locus_tag="VQ495_002085"
     CDS             complement(<1..>330)
                     /locus_tag="VQ495_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531975.1"
                     /translation="TVLSRLGDLSLPCPQDEDLTILPHSAYSETLYQAGSWSHKRRVC
                     QFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIKEMKEGFFGDKTDSS
                     TLIKNEVR"
BASE COUNT           87 a           73 c           79 g           91 t
ORIGIN      
        1 acgaacttcg tttttgatta aggttgaact atccgtttta tcgccaaaaa atccctcctt
       61 catctccttg atgaaattct cggcttgacc acgtccacga taaagctgaa actggtcttg
      121 gcttgttcca ctcgtcatat ttgtaacgag agaaataaca tcgtagaaca agtttccttc
      181 ttttcgttca gagaactggc agacacgacg cttgtgcgac caagatcctg cttgatagag
      241 agtttctgag taggcggagt ggggcaagat ggttaagtcc tcatcctgtg ggcaagggag
      301 ggaaaggtct ccaagacggc tcagaacagt
//
LOCUS       DAVBCG010000248          328 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVBCG010000248 DAVBCG010000000
VERSION     DAVBCG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.248"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT          114 a           54 c           58 g          102 t
ORIGIN      
        1 ttactatata atccttagaa aacatatcgg acaagcggtt gaattccgtt ccgtccatta
       61 gtagataaag gattactcct tcttgaaatg acagatgaac ttgtttctta taaaaatgtt
      121 gtctgaaact attagccttt ttccagaagt tttagaggaa gaaaacttta cgaggaaaaa
      181 ggagttactc aaataacaat tcaggattaa aaatagacag ttgaggagcg gaaggtataa
      241 attaagtttg ctactgtata atggatttat cactaatact cttcgaaaat ctcttcaaac
      301 cacgtcagct tcgccttgcc gtagatat
//
LOCUS       DAVBCG010000249          309 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVBCG010000249 DAVBCG010000000
VERSION     DAVBCG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.249"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..>309)
                     /locus_tag="VQ495_002086"
     CDS             complement(<1..>309)
                     /locus_tag="VQ495_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531976.1"
                     /translation="KSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDK
                     ILSYSEDLKHHYQIYQLLLFHFQNKDPEKFFGLIEDNLKQVHPLFQTVFKTFLKNKEK
                     "
BASE COUNT           91 a           44 c           64 g          110 t
ORIGIN      
        1 attttctctt tgttctttag aaaggtttta aagacagtct gaaaaagagg atgaacctgc
       61 ttcagattgt cctcaatgag tccgaaaaat ttctcagggt ctttgttctg aaagtgaaaa
      121 agtaagagtt gatagatctg atagtggtgt ttcaagtctt ctgaatagct taaaatcttg
      181 tcaagaattt ctttatttgt taagtgcatg cgaaaagtag ggcgataaaa acgtttatcg
      241 ctcaatttac gactatcctg ttggatgagt ttccagtaac gcttgatagc cttgtattca
      301 tgagatttt
//
LOCUS       DAVBCG010000250          295 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVBCG010000250 DAVBCG010000000
VERSION     DAVBCG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.250"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>295
                     /locus_tag="VQ495_002087"
     CDS             <1..>295
                     /locus_tag="VQ495_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HET2531977.1"
                     /translation="ESDGSRHWKALSTDFKIGVISAGEYYFSADLYATDLGTHIKFGF
                     YYHNSTGKLNFYAGRTKIEVTEKGRWTRLGIDLKVNDDIDLTKKVQFYIYGYNF"
BASE COUNT           96 a           55 c           59 g           85 t
ORIGIN      
        1 cgagagcgac ggctcccgac actggaaagc attgtcaact gattttaaaa tcggagtcat
       61 ctcagctgga gagtattact tttcagctga tttgtatgcg acagatttag gcactcatat
      121 caaatttgga ttttactatc acaactcgac tggcaaactc aatttttacg caggtcgaac
      181 aaaaattgaa gttactgaaa aaggccgttg gactaggcta ggaattgact taaaggtcaa
      241 tgatgacatt gacctgacaa aaaaagtcca attttacatt tacggctata atttt
//
LOCUS       DAVBCG010000251          292 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVBCG010000251 DAVBCG010000000
VERSION     DAVBCG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.251"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            51..>292
                     /locus_tag="VQ495_002088"
     rRNA            51..>292
                     /locus_tag="VQ495_002088"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           90 a           54 c           81 g           67 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cg
//
LOCUS       DAVBCG010000252          288 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVBCG010000252 DAVBCG010000000
VERSION     DAVBCG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.252"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>288
                     /locus_tag="VQ495_002089"
     CDS             <1..>288
                     /locus_tag="VQ495_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2531978.1"
                     /translation="NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEW
                     FDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT           93 a           59 c           59 g           77 t
ORIGIN      
        1 caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt atgccttaca atcttcaata
       61 tactgtagaa gtcaaaaacg gtagtttaat catacctcat tatgaccatt accataacat
      121 caaatttgag tggtttgacg aaggccttta tgaggcacct aaggggtata ctcttgagga
      181 tcttttggcg actgtcaagt actatgtcga acatccaaac gaacgtccgc attcagataa
      241 tggttttggt aacgctagcg accatgttca aagaaacaaa aatggtca
//
LOCUS       DAVBCG010000253          286 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVBCG010000253 DAVBCG010000000
VERSION     DAVBCG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.253"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT          101 a           53 c           55 g           77 t
ORIGIN      
        1 atgacaagcc gacgctgacg tggtttgaag aaatttttga agagtatcat tcaccatttc
       61 acaactaaat cgaagacttt ctgccgtaat gaaaacacct gaaacagctt ggtttcagct
      121 gtccggaaac tttaagactt aggttcaaag tttaggtatg gaacttcgaa ggaggtcgct
      181 accgtccgtc attacttagg aaagtcttaa aaaatctata aagtaaaaag agcccgacag
      241 ctctttttgt ttatactcaa tgaaaatcaa aaagcaaact aggaaa
//
LOCUS       DAVBCG010000254          277 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVBCG010000254 DAVBCG010000000
VERSION     DAVBCG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.254"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>277
                     /locus_tag="VQ495_002090"
     CDS             <1..>277
                     /locus_tag="VQ495_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2531979.1"
                     /translation="QRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          126 a           37 c           47 g           67 t
ORIGIN      
        1 aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aaggaga
//
LOCUS       DAVBCG010000255          276 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVBCG010000255 DAVBCG010000000
VERSION     DAVBCG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.255"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..196)
                     /locus_tag="VQ495_002091"
     CDS             complement(<1..196)
                     /locus_tag="VQ495_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl pyrophosphate isomerase"
                     /protein_id="HET2531980.1"
                     /translation="MLEILKEYLLLEEGDYIFTNNGSPLMITCFNYFLKNSFRKSEIK
                     KDDFVLTAHVFRYSHISLLAE"
BASE COUNT          107 a           49 c           26 g           94 t
ORIGIN      
        1 attcagcaag caaacttata tgcgaatatc taaaaacatg agcagtcaat acaaaatcat
       61 ctttcttaat ttcacttttc ctaaaactgt ttttcaaaaa gtaattgaaa caagttatca
      121 ttaagggaga accattattt gtaaatatat aatcaccttc ttctaaaaga agatattctt
      181 ttaaaatttc taacactcta tcactaatac caattactct atatgaagct aaacttttcg
      241 gactatccaa aaatacttcg ttattggacg attaca
//
LOCUS       DAVBCG010000256          272 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVBCG010000256 DAVBCG010000000
VERSION     DAVBCG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.256"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            1..272
                     /locus_tag="VQ495_002092"
                     /pseudo
     CDS             1..272
                     /locus_tag="VQ495_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT           88 a           57 c           58 g           69 t
ORIGIN      
        1 ctgcagaggc aggagaattt gttacaattg atgccttatt tcaggcttat aaaaaggagt
       61 taggtcgttc ctacacacgt gatgccttct atcaactgtt gaagcgccat ggttggcgaa
      121 atattacgcc acgtccagaa catcctaaga aagcagacgc tcaaaccatt gtcgcgtcta
      181 aaaataaaat ctcaattcaa gaagacaaga aagcgcttta aaaccagtag accttttcat
      241 aaggttcgct tgatgtacca agatgaggct gg
//
LOCUS       DAVBCG010000257          266 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVBCG010000257 DAVBCG010000000
VERSION     DAVBCG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.257"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..186
                     /locus_tag="VQ495_002093"
     CDS             <1..186
                     /locus_tag="VQ495_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531981.1"
                     /translation="IFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKLD
                     FAYQEVHSNNGLKKYEK"
BASE COUNT           99 a           33 c           44 g           90 t
ORIGIN      
        1 atttttacaa aacaaattcg tcattggatt cttagtgatc aagttatttc agggaaaaga
       61 aaacttttct tccgggaaga tactccaaaa gagattttag atttgtacga aaacattaaa
      121 tctaaacttg attttgctta ccaagaagtt cattctaata atgggttgaa aaaatatgaa
      181 aaatagaata attgatgttt ttgaagtagt aaatcgcctc cttgtaataa ctgttgaaaa
      241 tcctgatttc gaagacttga gagtta
//
LOCUS       DAVBCG010000258          263 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVBCG010000258 DAVBCG010000000
VERSION     DAVBCG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.258"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT          107 a           35 c           36 g           85 t
ORIGIN      
        1 aaaataaaca aaaagacata cttcatatct gcaagggcag atgttccagt caggtataaa
       61 gttatgtttg gcagaaatct cttcgatgac ttttcgaaaa taaaaaacaa taaagatgaa
      121 actctatcac tttgtctatt attttgtatg acagacaaat cgtcagaaga attctgtaaa
      181 ttttttattg aaaataattc atatataaca agtagacatc actatgaggc aataagatta
      241 tttgaaaaac attttatctt taa
//
LOCUS       DAVBCG010000259          254 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVBCG010000259 DAVBCG010000000
VERSION     DAVBCG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.259"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..>254)
                     /locus_tag="VQ495_002094"
     CDS             complement(<1..>254)
                     /locus_tag="VQ495_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531982.1"
                     /translation="HQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSF
                     IAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIIT"
BASE COUNT           83 a           47 c           54 g           70 t
ORIGIN      
        1 gtaatgattt tcacttgaca acgaacggct ctatcgtagc gaagaaagtg atttcggatg
       61 acagcttgtg ttctgccttc aagaacagtg ataatattaa gattatcaaa atcttgcgca
      121 atgaaactca tctttccctt agtgaaggca tactcatccc aagacataat ctttggaagc
      181 cgagaaaaat catgctcaaa gtgaaagtca ttgagcttgc gaatgacagt tgaagttgaa
      241 atggccagct gatg
//
LOCUS       DAVBCG010000260          248 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVBCG010000260 DAVBCG010000000
VERSION     DAVBCG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.260"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..>248)
                     /locus_tag="VQ495_002095"
     CDS             complement(<1..>248)
                     /locus_tag="VQ495_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531983.1"
                     /translation="TNALSAIGQPLLEMMIPVFQTLATIVKGVAELFNSLPDPVKDFV
                     VILGTVVTAVGVIAPIFLTLQALAEFLKISIGEMIIAA"
BASE COUNT           86 a           50 c           48 g           64 t
ORIGIN      
        1 aatgcggcaa ttatcatttc tccaatagat atttttaaaa actcagcaag ggcttgcaag
       61 gttaagaata tgggggctat gacccctaca gcagtcacaa ctgttcctaa aataacaaca
      121 aaatctttta ctggatcagg caaggaattg aacagctcgg ccacaccttt cacaatcgtt
      181 gccaaggttt gaaaaacagg aatcatcatt tctagaagag gttgaccaat agcagataat
      241 gcattggt
//
LOCUS       DAVBCG010000261          244 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVBCG010000261 DAVBCG010000000
VERSION     DAVBCG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.261"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT           69 a           32 c           58 g           85 t
ORIGIN      
        1 gattctctga agttatggat gtcgttccaa tatgtgtacg ttggaatgtt agtgcttata
       61 tggggggaac agaatcctct cttgattgaa gacaagttag ccattaggct ggtttgtctc
      121 tttgtcaact gtagtgggtt gatataatag tattagtgag tgggataaaa gtttcattta
      181 gtttattcag tacaaattta acgggtcaag atttattgaa ctgcacccca atagttagac
      241 agaa
//
LOCUS       DAVBCG010000262          229 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVBCG010000262 DAVBCG010000000
VERSION     DAVBCG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.262"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>229
                     /locus_tag="VQ495_002096"
     CDS             <1..>229
                     /locus_tag="VQ495_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2531984.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDH"
BASE COUNT           72 a           49 c           55 g           53 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatc
//
LOCUS       DAVBCG010000263          227 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVBCG010000263 DAVBCG010000000
VERSION     DAVBCG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.263"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..>227)
                     /locus_tag="VQ495_002097"
     CDS             complement(<1..>227)
                     /locus_tag="VQ495_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2531985.1"
                     /translation="FLKPLYNLLRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSG
                     KVENQAITLYHHDQSRSGSVVQEFLGDYSGYV"
BASE COUNT           69 a           50 c           49 g           59 t
ORIGIN      
        1 acatagccag aataatctcc taggaattct tgtactaccg aaccactccg actctgatca
       61 tgatggtaca gcgtgattgc ttgattctca actttcccag acaaaaaggt ccaatagtag
      121 gtcagatggc tatgactctc tagaacccga taagaagttt catccgcatg aagtaagggc
      181 tgagtcaata gtctctctcg caagaggtta taaaggggct ttaggaa
//
LOCUS       DAVBCG010000264          224 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVBCG010000264 DAVBCG010000000
VERSION     DAVBCG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.264"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            complement(<1..173)
                     /locus_tag="VQ495_002098"
     CDS             complement(<1..173)
                     /locus_tag="VQ495_002098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531986.1"
                     /translation="MLAEAEALVLAEADALVEADSDALVEAEADALVDAEAEALVDAE
                     AEALVDAEAEALVL"
BASE COUNT           52 a           75 c           53 g           44 t
ORIGIN      
        1 agtactagtg cctcagcctc agcatcaaca agcgcctcag cttcagcgtc aaccagtgcc
       61 tcggcttcag catcaacgag tgcgtcagct tcagcctcaa cgagtgcgtc tgagtcagcc
      121 tcaacgagtg cgtcggcttc agcaagtacc agtgcttcgg cctcagcaag cacaagtgcg
      181 tctgaatccg catcaacgtc tgcctcagcc tcagcatcga caag
//
LOCUS       DAVBCG010000265          210 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVBCG010000265 DAVBCG010000000
VERSION     DAVBCG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.265"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>210
                     /locus_tag="VQ495_002099"
     CDS             <1..>210
                     /locus_tag="VQ495_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2531987.1"
                     /translation="DAILGVTESAQEIALEKALQNADLDFDHKAGLLRQEISDDIELA
                     KARAEEVKRELSDTINQRFNSFDNGP"
BASE COUNT           69 a           42 c           48 g           51 t
ORIGIN      
        1 cgatgcaatt cttggagtga cagaatctgc acaagaaatc gcccttgaaa aggctcttca
       61 aaatgctgac ttagactttg atcataaggc tggattgctt agacaggaaa tttctgacga
      121 tattgaactg gccaaagcca gagctgaaga agtcaagaga gaactgtctg acactatcaa
      181 tcagcgattt aatagctttg acaacgggcc
//
LOCUS       DAVBCG010000266          208 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVBCG010000266 DAVBCG010000000
VERSION     DAVBCG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.267"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
     gene            <1..>208
                     /locus_tag="VQ495_002100"
     CDS             <1..>208
                     /locus_tag="VQ495_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET2531988.1"
                     /translation="HVETQGTIGVENELSQEQIDAADVVILAVDVKISGMERFEGKKI
                     IKVPTEVAVKSPNKLIAKAVEIVT"
BASE COUNT           68 a           26 c           55 g           59 t
ORIGIN      
        1 ttcatgttga gactcagggg acaatagggg tagaaaatga attgagtcaa gagcagattg
       61 atgcagcgga tgtagttatt ttagcagttg atgttaagat ttctggtatg gaacgctttg
      121 agggtaaaaa gattatcaag gttccaacag aagtggcagt caaatctccc aataaactga
      181 ttgctaaagc tgttgagatt gttacgaa
//
LOCUS       DAVBCG010000267          202 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVBCG010000267 DAVBCG010000000
VERSION     DAVBCG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.268"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT           79 a           21 c           27 g           75 t
ORIGIN      
        1 attctgctaa aaaagaggtt gaaaaagtat tagaagattc attgaaaaag ctagagcata
       61 aataaaacag tctcttttta atgctatcgt aaatactgta ttgtgtagtt attagtaaac
      121 ctatatattg tccccaaatt tatttagatt attctctagg attttacttg attttaaaga
      181 aaatatagtt taacatataa at
//
LOCUS       DAVBCG010000268          200 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain MN06303
            SAMEA1919820-rid20182883.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVBCG010000268 DAVBCG010000000
VERSION     DAVBCG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1919820
            Sequence Read Archive: ERR298105
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980270.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980270.1
            Genome Coverage       :: 44x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:42:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,100
            CDSs (total)                      :: 2,063
            Genes (coding)                    :: 1,961
            CDSs (with protein)               :: 1,961
            Genes (RNA)                       :: 37
            rRNAs                             :: 3, 3 (16S, 23S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 58 of 102
            Pseudo Genes (incomplete)         :: 42 of 102
            Pseudo Genes (internal stop)      :: 27 of 102
            Pseudo Genes (multiple problems)  :: 21 of 102
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1919820-rid20182883.denovo.269"
                     /strain="MN06303"
                     /db_xref="taxon:1313"
BASE COUNT           88 a           19 c           27 g           66 t
ORIGIN      
        1 ctattaaaac agaagatttt aatttctctt ctagatatgt aactgactat aaaaatcttg
       61 agaatgctga ttcatcaaaa gaaaaagtat ataaaaatat tgaaaaacta ttaccgtttt
      121 ataatagaga aacaattgtt aagtacggaa atttagtaga gacaagtagt aatctttata
      181 ataaagaatt attgtcagta
//
